CPS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CPS1
Entrez Gene ID	510506
Full Name	carbamoyl-phosphate synthase 1
General protein information	Preferred Names carbamoyl-phosphate synthase 1 Names carbamoyl-phosphate synthase [ammonia], mitochondrial carbamoyl-phosphate synthase 1, mitochondrial carbamoyl-phosphate synthetase 1, mitochondrial
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015458817.1	XP_015314303.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
XM_025000619.1	XP_024856387.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
XM_010802252.3	XP_010800554.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
XM_025000627.1	XP_024856395.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
XM_010802252.2	XP_010800554.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
NM_001192258.1	NP_001179187.1	carbamoyl-phosphate synthase [ammonia], mitochondrial
XM_015458817.2	XP_015314303.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPS1	NP_001179187.1
Xenopus tropicalis (tropical clawed frog)	cps1	XP_004917905.1
Homo sapiens (human)	CPS1	NP_001116105.1
Rattus norvegicus (Norway rat)	Cps1	NP_058768.1
Pan troglodytes (chimpanzee)	CPS1	XP_001146604.2
Canis lupus familiaris (dog)	CPS1	XP_545627.2
Mus musculus (house mouse)	Cps1	NP_001074278.1
Danio rerio (zebrafish)	cps1	XP_683282.4
	CPS1	XP_001110375.1
Gallus gallus (chicken)	CPS1	NP_001039306.1
Arabidopsis thaliana (thale cress)	CARB	NP_564338.1
Oryza sativa (rice)	Os01g0570700	NP_001043373.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CPS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa38271	NM_001192258.1 Latest version!	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa38271	XM_025000627.1 Latest version!	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa38271	XM_015458817.2 Latest version!	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa38271	XM_010802252.3 Latest version!	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa38271	XM_025000619.1 Latest version!	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa38271	XM_010802252.2	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa38271	XM_015458817.1	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CPS1 ( NM_001192258.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179187.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), mRNA.

Price & Availability↑

CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	NM_001192258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jul 17, 2010 this sequence version replaced XM_587645.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.42606.1, SRR5571260.43926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145427, SAMN03145482 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179187.1
CDS	122..4624
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	NM_001192258.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192258.1

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPS1 ( XM_015458817.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314303.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	XM_015458817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314303.1
CDS	35..4537
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	XM_015458817.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458817.1

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPS1 ( XM_025000619.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856387.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA.

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CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	XM_025000619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856387.1
CDS	286..4788
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	XM_025000619.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000619.1

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPS1 ( XM_010802252.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800554.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

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CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	XM_010802252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800554.1
CDS	397..4899
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	XM_010802252.3
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802252.3

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPS1 ( XM_025000627.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856395.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA.

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CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	XM_025000627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856395.1
CDS	52..4554
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	XM_025000627.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000627.1

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPS1 ( XM_010802252.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800554.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	XM_010802252.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800554.1
CDS	105..4607
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	XM_010802252.2
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802252.2

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPS1 ( XM_015458817.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314303.1 Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa38271
Clone ID Related Accession (Same CDS sequence)	XM_015458817.1 , XM_025000619.1 , XM_010802252.3 , XM_025000627.1 , XM_010802252.2 , NM_001192258.1 , XM_015458817.2
Accession Version	XM_015458817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314303.1
CDS	228..4730
Translation	MTRILTACKVVKTLKTSFGLTNVTAHHQWKFARPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLAGYPEALTDPAYKGQILTMANPIVGNGGAPDTAALDELGLSKYLESDGIKVAGLLVLNYSDDYHHWLATKSLGQWLREEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEISTKDVKVYGKGNPMKVVAVDCGIKNSVIRLLVKRGAEVHVVPWNHDFTKMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNLITGLAAGAQVYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDSTLPAGWKPLFVNVNDQTNEGIMHESKPFFGVQFHPEVGPGPTDTEYLFDSFVSLIKKEKGTTITSVLPKPGLVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFRRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADNIGYPVMIRSAYALGGLGSGLCPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDANDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQLLRRVSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIDGFTSRLPMNKEWPSDIDLRRELADPSSIRIYAIAKALEDNMSLDEIMKLTSIDKWFLYKMRDILNMEKTLKGLNSESITEETLKKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDINFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVGFPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGIDFIDVATKVMIGEHIDEKPLPTLEHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKLPQKGILIGIQQSFRPQFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSPIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVKKARNVDSKSLFHYRQYRAGKEL

Target ORF information:

RefSeq Version	XM_015458817.2
Organism	Bos taurus(cattle)
Definition	Bos taurus carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458817.2

ORF Insert Sequence:

ATGACAAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGACAAG CTTTGGCTTA 
ACCAATGTGA CGGCACACCA TCAATGGAAA TTTGCAAGAC CTGGCATCAG ACTCCTTTCT 
GTCAAGGCAC AAACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC 
TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGCAGGGTAC 
CCGGAAGCTC TTACTGACCC TGCTTACAAG GGGCAGATTC TTACCATGGC CAATCCTATT 
GTTGGGAATG GTGGAGCCCC AGATACTGCT GCACTGGATG AACTGGGACT CAGCAAATAT 
TTGGAATCTG ATGGGATTAA GGTTGCAGGT TTGCTGGTGC TGAATTATAG TGATGACTAC 
CACCACTGGC TGGCTACCAA GAGTCTAGGA CAGTGGCTAC GGGAAGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GAGATAAGGG TACCATGCTT 
GGTAAGATTG AATTTGAAGG TCAGTCTGTG GATTTTGTGG ATCCAAATAA GCAGAATTTG 
ATTGCTGAGA TTTCAACCAA GGATGTCAAG GTATACGGCA AAGGAAATCC CATGAAAGTG 
GTAGCTGTAG ACTGTGGGAT AAAAAACAGT GTAATCCGCT TATTAGTAAA GCGAGGGGCA 
GAAGTGCATG TAGTGCCTTG GAATCATGAT TTCACCAAGA TGGAGTATGA TGGGCTTTTA 
ATTGCTGGAG GACCTGGTAA CCCAGCTCTT GCACAACCAC TAATTCAGAA TGTAAAAAAG 
ATCTTGGAGA GTGATCGCAA GGAACCATTG TTTGGAATCA GTACAGGAAA TTTAATAACA 
GGATTGGCTG CTGGTGCCCA AGTCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAA 
CCTGTTTTGA ATATCACAAA TAGACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC 
CTGGACAGCA CCCTTCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTTAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCC TTCTTTGGTG TGCAGTTCCA CCCAGAAGTT 
GGCCCAGGGC CAACAGACAC TGAGTATCTG TTTGATTCCT TTGTCTCACT GATAAAGAAG 
GAGAAAGGAA CCACCATTAC ATCGGTACTA CCAAAGCCAG GCCTAGTAGC ATCTCGAGTT 
GAGGTTTCCA AAGTCCTTAT CCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCGCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTC 
CTGATGAACC CAAATATTGC ATCGGTGCAA ACCAATGAAG TGGGCTTAAA GCAAGCAGAT 
ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA AGCGGAACGG 
CCAGATGGGT TAATTCTAGG CATGGGTGGC CAGACAGCTC TGAATTGTGG AGTGGAATTA 
TTCAGGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAATCT 
ATTATGGCCA CAGAAGATAG ACAGCTGTTC TCAGACAAAC TAAATGAAAT TAATGAAAAA 
ATTGCTCCAA GCTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACAACATT 
GGCTACCCGG TGATGATCCG TTCTGCTTAC GCCCTGGGCG GGTTAGGCTC AGGCCTCTGT 
CCTAATAGGG AGACTTTAAT GGACCTCAGC ACAAAGGCTT TTGCTATGAC CAACCAGATT 
CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAGATAGAAT ATGAAGTGGT CCGAGATGCT 
AATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCATACA 
GGTGACTCAG TTGTCGTGGC CCCTGCCCAG ACACTCTCCA ATGCGGAGTT TCAGTTGTTG 
AGACGTGTTT CAATCAATGT CGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAA 
TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGATTGTCC 
CGAAGCTCTG CCCTGGCCTC AAAAGCTACC GGTTACCCAC TGGCATTCAT CGCTGCAAAG 
ATTGCCCTGG GAATTCCACT TCCAGAAATC AAGAATGTTG TATCAGGGAA GACATCAGCC 
TGCTTTGAAC CTAGCCTGGA TTACATGGTT ACCAAGATTC CCCGCTGGGA TCTTGATCGT 
TTTCATGGAA CTTCTAGCCG AATTGGTAGT TCTATGAAAA GTGTAGGAGA GGTCATGGCT 
ATTGGTCGCA CCTTTGAGGA GAGTTTTCAG AAGGCATTAC GGATGTGCCA TCCTTCTATA 
GATGGTTTTA CTTCCCGTCT CCCAATGAAC AAAGAGTGGC CATCTGACAT CGATCTCAGA 
AGAGAGCTGG CTGACCCATC CAGCATCCGC ATCTATGCCA TTGCCAAGGC CTTGGAAGAC 
AACATGTCAC TTGATGAGAT TATGAAGCTC ACATCCATTG ACAAGTGGTT TCTGTATAAG 
ATGCGTGACA TTTTAAACAT GGAAAAGACA CTGAAAGGGC TCAACAGTGA GTCCATTACA 
GAAGAAACCC TGAAAAAGGC AAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGC 
CTCGGGCTGA CTGAGGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT TCATCCTTGG 
GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTCTATGTT 
ACCTACAATG GTCAGGAGCA TGATATCAAT TTTGATGATC ATGGAATGAT GGTGCTGGGC 
TGTGGTCCAT ATCACATTGG TAGCAGCGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACATTGC GTCAGCTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC TGAGACCGTG 
AGCACAGACT TTGATGAGTG TGACAAATTG TACTTTGAAG AGTTGTCTTT GGAAAGAATC 
CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG GGGCCAGATT 
CCAAACAACC TGGCAGTCCC CCTGTACAAG AATGGCGTCA AGATCATGGG CACGAGCCCT 
CTGCAGATTG ACAGGGCTGA GGACCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG 
GTGGCTCAGG CACCATGGAA AGCTGTTAAC ACTTTGAATG AGGCATTGGA ATTTGCGAAG 
TCTGTGGGCT TCCCCTGCTT GTTGAGACCT TCCTATGTTT TGAGCGGGTC TGCCATGAAT 
GTGGTATTCT CCGAAGACGA GATGAAAAAA TTCCTAGAGG AGGCCACTAG AGTGTCTCAG 
GAACACCCAG TGGTGCTGAC GAAATTTATT GAGGGGGCCC GGGAAGTGGA AATGGATGCT 
GTCGGCAAAG ATGGACGAGT CATCTCCCAT GCCATTTCTG AACACGTGGA GGATGCGGGC 
GTCCACTCGG GGGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCTAC CCGAAAGATC GCAAAGGCTT TTGCCATCTC TGGCCCATTC 
AACGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CCTGAGAGCT 
TCTCGATCCT TCCCCTTTGT TTCCAAGACA CTTGGAATCG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TTGGAGAGCA CATTGATGAG AAACCCCTTC CAACACTGGA GCATCCCATA 
ATTCCAGCCG ACTATGTTGC AATTAAGGCT CCAATGTTTT CCTGGCCCCG GTTGAGAGAT 
GCTGACCCCA TTCTACGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA 
GGTATTCATA CAGCTTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGCT ACCCCAGAAA 
GGCATCCTGA TTGGAATTCA GCAATCATTC CGTCCACAAT TCCTTGGTGT GGCTGAACAA 
TTACACAATG AAGGCTTCAA GCTCTTTGCC ACGGAAGCCA CATCAGACTG GCTCAATGCC 
AACAATGTAC CTGCTACCCC AGTGGCATGG CCATCTCAAG AAGGACAGAA TCCCAGCCTC 
TCACCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TGGTGATTAA CCTCCCCAAT 
AACAACACTA AATTTGTCCA CGATAATTAT GTGATTCGGA GGACAGCTGT TGACAGTGGA 
ATTGCTCTCC TCACTAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GAAGAAAGCT 
CGCAATGTGG ACTCCAAGAG TCTCTTCCAC TACAGGCAGT ACAGAGCTGG GAAAGAACTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CPS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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