NIN gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_003582709.4	XP_003582757.1	ninein isoform X4
XM_015473159.1	XP_015328645.1	ninein isoform X2
XM_003586563.4	XP_003586611.1	ninein isoform X4
XM_015473161.1	XP_015328647.1	ninein isoform X6
XM_024997984.1	XP_024853752.1	ninein isoform X3
XM_024997985.1	XP_024853753.1	ninein isoform X4
XM_015473157.1	XP_015328643.1	ninein isoform X1
XM_015465060.1	XP_015320546.1	ninein isoform X1
XM_024997982.1	XP_024853750.1	ninein isoform X1
XM_015473156.1	XP_015328642.1	ninein isoform X1
XM_015473158.1	XP_015328644.1	ninein isoform X1
XM_005211736.3	XP_005211793.1	ninein isoform X3
XM_015473160.1	XP_015328646.1	ninein isoform X5
XM_015473162.1	XP_015328648.1	ninein isoform X7
XM_015465065.1	XP_015320551.1	ninein isoform X6
XM_002700603.5	XP_002700649.1	ninein isoform X8
XM_024997986.1	XP_024853754.1	ninein isoform X5
XM_002690835.4	XP_002690881.1	ninein isoform X8
XM_015465062.1	XP_015320548.1	ninein isoform X1
XM_005193648.3	XP_005193705.1	ninein isoform X3
XM_024997983.1	XP_024853751.1	ninein isoform X2
XM_024997987.1	XP_024853755.1	ninein isoform X6
XM_024997988.1	XP_024853756.1	ninein isoform X7
XM_015465061.1	XP_015320547.1	ninein isoform X1
XM_015465063.1	XP_015320549.1	ninein isoform X2
XM_015465064.1	XP_015320550.1	ninein isoform X5
XM_015465066.1	XP_015320552.1	ninein isoform X7
XM_005211736.4	XP_005211793.1	ninein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219840	XM_024997988.1 Latest version!	Bos taurus ninein (NIN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa06510	XM_024997985.1 Latest version!	Bos taurus ninein (NIN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa219839	XM_024997987.1 Latest version!	Bos taurus ninein (NIN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa47656	XM_024997984.1 Latest version!	Bos taurus ninein (NIN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91690	XM_015473157.1 Latest version!	Bos taurus ninein (NIN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91690	XM_015473158.1 Latest version!	Bos taurus ninein (NIN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91690	XM_015465060.1 Latest version!	Bos taurus ninein (NIN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91690	XM_015465061.1 Latest version!	Bos taurus ninein (NIN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91690	XM_015473156.1 Latest version!	Bos taurus ninein (NIN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91690	XM_015465062.1 Latest version!	Bos taurus ninein (NIN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91691	XM_015473159.1 Latest version!	Bos taurus ninein (NIN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91691	XM_015465063.1 Latest version!	Bos taurus ninein (NIN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91692	XM_015473160.1 Latest version!	Bos taurus ninein (NIN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91692	XM_015465064.1 Latest version!	Bos taurus ninein (NIN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91693	XM_015465065.1 Latest version!	Bos taurus ninein (NIN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91693	XM_015473161.1 Latest version!	Bos taurus ninein (NIN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91694	XM_015465066.1 Latest version!	Bos taurus ninein (NIN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa91694	XM_015473162.1 Latest version!	Bos taurus ninein (NIN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa06508	XM_024997983.1 Latest version!	Bos taurus ninein (NIN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06508	XM_005211736.4 Latest version!	Bos taurus ninein (NIN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06508	XM_005193648.3 Latest version!	Bos taurus ninein (NIN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06509	XM_003582709.4 Latest version!	Bos taurus ninein (NIN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06509	XM_003586563.4 Latest version!	Bos taurus ninein (NIN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06509	XM_024997982.1 Latest version!	Bos taurus ninein (NIN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06511	XM_024997986.1 Latest version!	Bos taurus ninein (NIN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06511	XM_002700603.5 Latest version!	Bos taurus ninein (NIN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa06511	XM_002690835.4 Latest version!	Bos taurus ninein (NIN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa06508	XM_005211736.3	Bos taurus ninein (NIN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

NIN ( XM_024997988.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853756.1 Bos taurus ninein (NIN), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa219840
Clone ID Related Accession (Same CDS sequence)	XM_024997988.1
Accession Version	XM_024997988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA ACTCTGTGGT TGGAACTAGC AGAGAAGGGT GCAGTTCTCT GCCTGAGATA 
GTGTGTGAAG GTAGAACATT CTCTTTCAAT GAATTTCATA GCATGGAGAC ATCTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853756.1
CDS	22..6021
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMENSVVGTSREGCSSLPEIVCEGRTFSFNEFHSMETSH

Target ORF information:

RefSeq Version	XM_024997988.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997988.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA ACTCTGTGGT TGGAACTAGC AGAGAAGGGT GCAGTTCTCT GCCTGAGATA 
GTGTGTGAAG GTAGAACATT CTCTTTCAAT GAATTTCATA GCATGGAGAC ATCTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_024997985.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853753.1 Bos taurus ninein (NIN), transcript variant X5, mRNA.

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CloneID	OBa06510
Clone ID Related Accession (Same CDS sequence)	XM_024997985.1
Accession Version	XM_024997985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGC CTTGCTACCG GAGCAGCGAG CAGTGCATGC AGATTCCTAC 
AGAAGGATTG GGCACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853753.1
CDS	22..6222
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_024997985.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997985.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGC CTTGCTACCG GAGCAGCGAG CAGTGCATGC AGATTCCTAC 
AGAAGGATTG GGCACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_024997987.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853755.1 Bos taurus ninein (NIN), transcript variant X7, mRNA.

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CloneID	OBa219839
Clone ID Related Accession (Same CDS sequence)	XM_024997987.1
Accession Version	XM_024997987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGAAC 
TCTGTGGTTG GAACTAGCAG AGAAGGGTGC AGTTCTCTGC CTGAGATAGT GTGTGAAGGT 
AGAACATTCT CTTTCAATGA ATTTCATAGC ATGGAGACAT CTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853755.1
CDS	22..6069
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMENSVVGTSREGCSSLPEIVCEGRTFSFNEFHSMETSH

Target ORF information:

RefSeq Version	XM_024997987.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997987.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGAAC 
TCTGTGGTTG GAACTAGCAG AGAAGGGTGC AGTTCTCTGC CTGAGATAGT GTGTGAAGGT 
AGAACATTCT CTTTCAATGA ATTTCATAGC ATGGAGACAT CTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_024997984.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853752.1 Bos taurus ninein (NIN), transcript variant X4, mRNA.

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CloneID	OBa47656
Clone ID Related Accession (Same CDS sequence)	XM_024997984.1
Accession Version	XM_024997984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AAGTGAGCTT CCCAGCTCTG CAAGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853752.1
CDS	22..6303
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQVSFPALQEY

Target ORF information:

RefSeq Version	XM_024997984.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997984.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AAGTGAGCTT CCCAGCTCTG CAAGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473157.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328643.1 Bos taurus ninein (NIN), transcript variant X2, mRNA.

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CloneID	OBa91690
Clone ID Related Accession (Same CDS sequence)	XM_015473157.1 , XM_015465060.1 , XM_015473156.1 , XM_015473158.1 , XM_015465062.1 , XM_015465061.1
Accession Version	XM_015473157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328643.1
CDS	301..6852
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015473157.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473157.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465060.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320546.1 Bos taurus ninein (NIN), transcript variant X1, mRNA.

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CloneID	OBa91690
Clone ID Related Accession (Same CDS sequence)	XM_015473157.1 , XM_015465060.1 , XM_015473156.1 , XM_015473158.1 , XM_015465062.1 , XM_015465061.1
Accession Version	XM_015465060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320546.1
CDS	217..6768
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015465060.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465060.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473156.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328642.1 Bos taurus ninein (NIN), transcript variant X1, mRNA.

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CloneID	OBa91690
Clone ID Related Accession (Same CDS sequence)	XM_015473157.1 , XM_015465060.1 , XM_015473156.1 , XM_015473158.1 , XM_015465062.1 , XM_015465061.1
Accession Version	XM_015473156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328642.1
CDS	217..6768
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015473156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473156.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473158.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328644.1 Bos taurus ninein (NIN), transcript variant X3, mRNA.

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CloneID	OBa91690
Clone ID Related Accession (Same CDS sequence)	XM_015473157.1 , XM_015465060.1 , XM_015473156.1 , XM_015473158.1 , XM_015465062.1 , XM_015465061.1
Accession Version	XM_015473158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328644.1
CDS	773..7324
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015473158.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473158.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465062.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320548.1 Bos taurus ninein (NIN), transcript variant X3, mRNA.

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CloneID	OBa91690
Clone ID Related Accession (Same CDS sequence)	XM_015473157.1 , XM_015465060.1 , XM_015473156.1 , XM_015473158.1 , XM_015465062.1 , XM_015465061.1
Accession Version	XM_015465062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320548.1
CDS	773..7324
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015465062.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465062.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465061.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320547.1 Bos taurus ninein (NIN), transcript variant X2, mRNA.

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CloneID	OBa91690
Clone ID Related Accession (Same CDS sequence)	XM_015473157.1 , XM_015465060.1 , XM_015473156.1 , XM_015473158.1 , XM_015465062.1 , XM_015465061.1
Accession Version	XM_015465061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320547.1
CDS	301..6852
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015465061.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465061.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AACTCTGCAA GAATACTAAA 
GCAGATGCAA TAGTGAAGGA TTTGTATGTG GAAAATGCCC AGTTGTTGAA AGCTCTGGAA 
ATGACTGAAC AGCGACAAAA AACAGCAGAG AAGAAAAATT ACCTCCTAGA GGAGAAGATT 
GCCAGCCTCA GTAATATAGT CAGGAATCTG ACACCAGCAC CACTGACTTC TACACCTCCC 
TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473159.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328645.1 Bos taurus ninein (NIN), transcript variant X4, mRNA.

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CloneID	OBa91691
Clone ID Related Accession (Same CDS sequence)	XM_015473159.1 , XM_015465063.1
Accession Version	XM_015473159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GGAAACCAGG AATACCTGGT AACTGTCATG GAGGAACGAA TGTTGGAAGT TGAACAGAAA 
TTGAAACTGG TGAAAAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAATACTA AAGCAGATGC AATAGTGAAG GATTTGTATG TGGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTA 
GAGGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGACACCAGC ACCACTGACT 
TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328645.1
CDS	216..6719
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015473159.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473159.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GGAAACCAGG AATACCTGGT AACTGTCATG GAGGAACGAA TGTTGGAAGT TGAACAGAAA 
TTGAAACTGG TGAAAAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAATACTA AAGCAGATGC AATAGTGAAG GATTTGTATG TGGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTA 
GAGGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGACACCAGC ACCACTGACT 
TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465063.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320549.1 Bos taurus ninein (NIN), transcript variant X4, mRNA.

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CloneID	OBa91691
Clone ID Related Accession (Same CDS sequence)	XM_015473159.1 , XM_015465063.1
Accession Version	XM_015465063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GGAAACCAGG AATACCTGGT AACTGTCATG GAGGAACGAA TGTTGGAAGT TGAACAGAAA 
TTGAAACTGG TGAAAAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAATACTA AAGCAGATGC AATAGTGAAG GATTTGTATG TGGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTA 
GAGGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGACACCAGC ACCACTGACT 
TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320549.1
CDS	216..6719
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_015465063.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465063.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GGAAACCAGG AATACCTGGT AACTGTCATG GAGGAACGAA TGTTGGAAGT TGAACAGAAA 
TTGAAACTGG TGAAAAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAATACTA AAGCAGATGC AATAGTGAAG GATTTGTATG TGGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTA 
GAGGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGACACCAGC ACCACTGACT 
TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473160.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328646.1 Bos taurus ninein (NIN), transcript variant X7, mRNA.

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CloneID	OBa91692
Clone ID Related Accession (Same CDS sequence)	XM_015473160.1 , XM_015465064.1
Accession Version	XM_015473160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AAGTGAGCTT CCCAGCTCTG 
CAAGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328646.1
CDS	217..6588
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQVSFPALQEY

Target ORF information:

RefSeq Version	XM_015473160.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473160.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AAGTGAGCTT CCCAGCTCTG 
CAAGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465064.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320550.1 Bos taurus ninein (NIN), transcript variant X7, mRNA.

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CloneID	OBa91692
Clone ID Related Accession (Same CDS sequence)	XM_015473160.1 , XM_015465064.1
Accession Version	XM_015465064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AAGTGAGCTT CCCAGCTCTG 
CAAGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320550.1
CDS	217..6588
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQVSFPALQEY

Target ORF information:

RefSeq Version	XM_015465064.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465064.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGG AAACCAGGAA 
TACCTGGTAA CTGTCATGGA GGAACGAATG TTGGAAGTTG AACAGAAATT GAAACTGGTG 
AAAAGGCTTC TTCAGGAGAA AGTGAATCAG CTCAAAGAAC AAGTGAGCTT CCCAGCTCTG 
CAAGAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473161.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328647.1 Bos taurus ninein (NIN), transcript variant X8, mRNA.

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CloneID	OBa91693
Clone ID Related Accession (Same CDS sequence)	XM_015473161.1 , XM_015465065.1
Accession Version	XM_015473161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGC CTTGCTACCG 
GAGCAGCGAG CAGTGCATGC AGATTCCTAC AGAAGGATTG GGCACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328647.1
CDS	218..6508
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_015473161.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473161.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGC CTTGCTACCG 
GAGCAGCGAG CAGTGCATGC AGATTCCTAC AGAAGGATTG GGCACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465065.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320551.1 Bos taurus ninein (NIN), transcript variant X8, mRNA.

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CloneID	OBa91693
Clone ID Related Accession (Same CDS sequence)	XM_015473161.1 , XM_015465065.1
Accession Version	XM_015465065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGC CTTGCTACCG 
GAGCAGCGAG CAGTGCATGC AGATTCCTAC AGAAGGATTG GGCACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320551.1
CDS	218..6508
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_015465065.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465065.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGCA 
CACCTTGGAG ATATTCCGGG TTCAGTTCCA GACAACCACA GTAAAGTTAG TCAGATGAAA 
TCCCTTGAAC GAGAATTAGA AACAATTCAT TTGGAAAATG AAGGCCTGAA AAAGAAACAA 
GTAAAACTGG ATGAACAGCT AATGGAGATG CAGCACCTGA GGTCCACTGT GATGCTTAGC 
CCATCCCCTC ATGCTTGGGA ACTGCAACTG CTCCAGCAGC AAACCTGTCC AGTGGTTCCC 
AGGGAGCAGT TTCTGCAGCT TCAACGTCAG CTGCTGCAGG CAGAAAGGAT AAACCAGTGC 
CTGCAGGAGG AACTTGAAAA TAGGACTTCT GAAACCAACA CACCACAGGC CTTGCTACCG 
GAGCAGCGAG CAGTGCATGC AGATTCCTAC AGAAGGATTG GGCACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015473162.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328648.1 Bos taurus ninein (NIN), transcript variant X9, mRNA.

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CloneID	OBa91694
Clone ID Related Accession (Same CDS sequence)	XM_015473162.1 , XM_015465066.1
Accession Version	XM_015473162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT GCAGATTCCT ACAGAAGGAT TGGGCACCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328648.1
CDS	217..6459
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_015473162.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473162.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT GCAGATTCCT ACAGAAGGAT TGGGCACCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_015465066.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320552.1 Bos taurus ninein (NIN), transcript variant X9, mRNA.

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CloneID	OBa91694
Clone ID Related Accession (Same CDS sequence)	XM_015473162.1 , XM_015465066.1
Accession Version	XM_015465066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT GCAGATTCCT ACAGAAGGAT TGGGCACCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320552.1
CDS	217..6459
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPASVGVSCQQAARVSGPLLNAHKLTHSLLIKDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_015465066.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465066.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGCATCT GTGGGTGTCA GCTGCCAACA AGCAGCTCGG 
GTGTCTGGGC CACTCCTGAA CGCTCACAAG CTGACACACT CTCTTCTGAT AAAAGACTGC 
ACTCTCGAAG CTCAGCCCAA ATATGTTAGA GGTGGGAAGC GTTATGGACG CAGATCTTTA 
CCTGAATTTC AAGAATCCGT GGAGGAATTT GCAGAGGTGA CTGTCATTGA GCCACTTGGT 
GAAGAAGCAC AGCCTTCACA CATCCCAGCC AGTGAGCGCA GCGAGCACTG GAAGACGCAG 
CGCAGCGAGG AGTATGAAGC AGAAGGCCAG TTAAGATTTT GGAACCCAGA TGATTTGAAA 
GCCTCACAGA CTGGGTCTTT GGCTCCCCAA GACTGGATAG AAGAGAAACT GCAGGAGGTT 
TGTGAAGATT TGGGGATCAC TCGTGATGGT CACCTAAACC GAAAGAAGCT GGTCTCCATC 
TGCGAGCAGT ATGGTTTGCA GAATGTCGAT GGAGAGATGC TTGAAGAAGT CTTCCATAAT 
CTTGATCCTG ATGGTATGAT GAGTGTAGAA GATTTTTTCT ATGGCTTGTT CAAAAATGGA 
AAACCTCTTA CACCATCAGC ATCTACCCCA TATAGGCAAT TGAAAAGGCA CATCTCCATG 
CAGTCTTTTG ATGAGAGTGG ACGACGGACC ACAGCCCCAT CTGCAATGAT GAGTACCATT 
GGTTTTCGGG TCTTCTCCTG CCTGGATGAT GGGATGGGCT ATGCATCAGT GGAGAGAATA 
CTTGACACCT GGCAGGAGGA AGGCATTGAA AACAGCCAGG AGATCCTGAA GGCCTTGGAT 
TTTAGCCTTG ATGGAAATGT CAACTTGACA GAGTTGACAC TGGCTCTTGA AAACGAACTT 
TTGGTCACCA AGAACAGCAT TCACCAGGCA GCTCTGGCCA GTTTTAAGGC TGAGATTCGG 
CATTTGCTGG AGCGAGTGGA TCAGATGCTC AGAGAAAAAG AGAAGCTACG GTCTGATCTG 
GACAAAGCTG AGAAGCTGAA GTCTCTAATG GCCTCAGAGG TGGACGATCA CCATGCAGCC 
ATAGAGCGGC GGAACGAGTA CAACCTCAGG AAACTGGATG AAGAGTACAA GGAGCGAATA 
GCAGCCTTAA AGAATGAGTT TCGAAAGGAG AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG 
CAGCGTTTGG AACTTGAGCA AGAAATCGAA AAGGCAAAAA CAGAAGAGAA CTACGTCCGG 
GACCGCCTCG CACTCTCTTT AAAGGAAAAC AGTCGTCTAG AAAATGAGCT TTTGGAGAAT 
GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG ACAAACAAAC TTCAGCGAAA TTTGGAAAAT 
GTTTTGGCAG AAAAGTTTGG TGACCTTGAT CCCAGCAGTG CTGAATTCTT CCTGCAAGAA 
GAAAGGCTGA CGCAGATGAG AAATGAATAT GAGCAGCAGT GCCGGCTATT ACAAGACCAA 
GTAGACGAAC TCCAATCTGA ACTGCAAGAA TATCGTACAC AAGGCAAAGT ATCCAGGCTT 
CCCTTGCAGA ACTCACTGTC AGAAGAACTT GATATTAACA GTGGTTGCAT AGAGCCTGAC 
CAGGGGCTTG GTTCTGAAGA ATGCAATCCA CTGAATATGA GCATCGAGGC AGAGCTGGTC 
ATCGAACAGA TGAAAGAACA ACATCACAGG GAGTTGTGTC ACCTAAGACT GGAGCTTGAA 
GATAAAGTGA ACTATTATGA AAAGCAGCTA GATGAAACCA AAGTTGCCTT TGAAAAGGAA 
CAGGAGAACA TGAAGCTCAA GTGTGAGAAT GAAGTACACA TCTTAGAAGA ACAAATAAGT 
GACCTTAAAA ATAAAATTGC AGAACTTCAG GGCCAGACAG AGGTACTCAA GGAGGCACAG 
CACGTGGCTA TCTGCAGACA TGAGGAGGAG AAAAAACAGC TGCAAATGAA GTGGGATGAG 
GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG CTGGAGCATG AGATGGCACT CAGGGCTAGG 
CTGGAGCAGG TGGAGGAAAG CTTTAACAGA GAGAGGGAAA GACTCGTCCA AAATGGCGCC 
TGGACAGAGG AGAAGGTGAG GGGCTTGACT CAGAAACTAG AACAAGTTCA CCAGGAGCAG 
CTGAAAAGCC TGGTGGAGAA ACACATTCTT GAGAAAGAGG AATTGCAAAA AGAGCTCTTG 
GAAAAACACC AAAGGGAACT ACATGAGGGA AGGGAAAAAA TGGAAACTGA GTGTAATAGA 
AGAACCTCTC AGATAGAAGC CCAGTTTCAG GCTGATTGTC AGAAAGTCAC TGAGAGATAC 
GAACACGCCC TGCGGAGTCT GGAGGTGCAC TACCGCCACC AGCTGAAGGA GCTCCTGGAT 
CAACAATGTG AGGAGAGGTC CCAGTGGGAG TTTGAGAAGG ACGAGCTCAC CCAGGAATGT 
GCAGAAGCCC AGGAGCAGCT TAAAGAGACT CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC 
CTCACCCAGG AGAGAGAGAT GCTGGAGAAA ACATACAAGG AGCATTTGAA TAGTATGGTT 
GTTGAGAGAG AGCAGCTACT ACAGGACCTG GAAGATCTGA GAAAAGTGTC TGAAAGTCAG 
CAAAGTCTCC TGTCCAACCA GATACTTGAG CTGAAGAGCA GTCATGAAAG AGAGCTGAAG 
GACCGTGAGC AGATCCTGTG CCAGGCAGGC ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT 
GAACAGCTGC AAAGGGAACA TGACCAGGAG AGGCAGGAAA TGATGTCCAA GCTTCTGGTC 
ATGGAGAGTG TCCACAGAGC AACCTGTGAG AAGGCAGATC GAGAGAGGGC TGAGATGAGC 
ACAGAAATCT CCAGGCTCCA GAATAAAATC AAGGAGATGG AGCAGGTAGT GTCTCCTCCC 
TCCAGGCTTC AGAATAGCTG TCAAGTGATA GGAGGGGAGG AGGCAGAAGA AAATGGTGCC 
ATGTCCCTGC TTCAGCAAGG AGAACAGCTG TTGGAAGAGA ATGGAGATGT TCTCTTAAGC 
CTGCAAAGAG CCCATGAACG AGCAGTGAAG GAAAATGTGA AAATGGCAAC TGAAATTTCT 
AGGTTGCAGC AGAGGCTGCA AAAATTAGAA CCAGAGTCAG TAATGTCTTC TTGTTTAGAT 
GAACCAGCTA CTGGGCTCTT TGGAAATTCA GTGGAACAAA CAGAGCCATT TTTACAGCCA 
AACCGAATCA AACAAGCAGA AGGTGGAACC ACACAGCGTA TTCTCAGTGA CCTACAAGGC 
GATGAGGTCC GTGACCTCGA AAGTACAGGG ACGAGCTCTG GGCAGAGACA GGTCAGAGTG 
GAGGAGTCTG AAGCATCAAT AGAGAGTTTT TCCGAGCTTG AAAATAGTGA GGAGACCAGA 
ACTGAAACCT GGGACCTGAA GAATCAGGTC GGCCAGCTTC GGGAACAGTT AATGATCTTA 
CGTGCAGACT GCGATCGAGC GTCTGAAAAG AAACAGGACC TACTGTTTGA TGTTTCTGTG 
CTAAAAAAGA AACTGAAGAT GCTTGAAAGA ATTCCTGAGG CTTCCCCCAA GTATAAAGTG 
TTATACGAAG ATGCCAGTAG AGAAAATGAA TGTCTTCAGG AAGAGCTGAG GATGATGGAG 
ACGCGCTATG ATGAAGCACT AGAAAGTAAC AAAGAGCTCA CTTCAGAAAT GTTCAGATTA 
CAGGATGAGC TAAAGAAGGT TGAAGAAATG ACTGACACAT TCCTTAGCCT GGAAAAGAGT 
TACAAGGAGA TCAAAAGAGA AAATGAGGAA CTGCATGTTC TGGTTTTGAG ACTTCAAGGC 
AAGATTGAGA AGCTACGAGA AAGAGCAGTG CTGCAGTGTG ACTGCTTCTC CTTATGGGAA 
GCCCATTTAG ATAACCTGGA AGTCGGACCT GATGAAAAGG TCTTTGAACT GAACCAAACA 
CTGGATGAGC GTGTACCAAA TGTTATGAGC GTGCACCATA TTATAGAAGA ACATTTGTAT 
CAAGAAAACC AGTACCTAGA ACAGGAGAAT ACACAGCTCT TGGAGAAAGT AAAAGCACAT 
GAAATTGCCT GGCTACACGG AACACTCCAG ACACATCGAG ACAAGCTTGG AGCACAGAAC 
CAAGTTGTAC TGGAGGAAAA CACTGCTCTC CTCGGCCTTC AAGACAAGCA TTTTCAGCAC 
CAGGCCACCA TCGCAGAGTT AGAACGGGAG AAAAAAAAGC TGCAGGAGCT GACTAGAAAG 
TTGAGGGAAA GAGTCACTGC TTTAGTCAAG CAAAAGGATG TACCTTCTCA AGGAGAAAAG 
GAGGAAGAGC TGAAGGCAAT GATGCATGAC CTGCAAATCA CATGCAGTGA GATGCAGCAG 
AAAGTTGAGC TTCTGAGATA TGAATCTGAG AAGCTTCAAG AGGAAAATTC TATTTTGAGA 
AATGAAATTA CTACTTTAAA TGAAGAAGAC AGCATTTCTA ACCTGAAATT AGGGAAATTA 
AGTGGATCTC AGGAAGAAAT GTGGCAAAAA ATAGAAACTG TAAAACAGGA AAAAGCTGCA 
GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA CAGATTTCAG AATTAAAAAC CAAAAACCAG 
CAGTTGGATT TGGAAAATAT GAAGCTTAGC CAAAAGAACT CTCAGAATCA GAAAGAGCTG 
CAAGAACTTA ATCAACGTCT GGCAGAAATG CTAAGCCAGA AGGACAAAGA GCCAGGGCAC 
AGTGCATGTG AGGAATGGGA ACAAGAAAAG TCTAATCTCA AAGAAGAACT GGAACACTGT 
AAATTGCAGT CTTCCACTTT AGTGTCTTCT TTGGAGGCAG AACTTTCTGA AGTTAAAATA 
CAGACTCACA TCGTGGAACA GGAAAACCTC CTTCTCAAAG ATGAATTGGA GAAAGTAAAA 
CAACTCTGCA GATGTCCTGA TCTCTCTGAC TTCCAGCAAA AAATTTCTAG TATTCTAAGC 
CACAATGAAA AACTGCTGAA AGAAAAGGAA GCTCTAAGTG AAGAATTAAA TATCTGTGTT 
GATAAGTTGG CAAAATCAAG TCTTTTAGAG CACAGAATTG CTACTATGAA GCAAGAACAG 
AAGTCTTGGG AACACCAGAG TGAAAGCTTA AAGTCACAAC TAGTGGCTTC ACAGGAAAAG 
GTTCAGAATT TAGAAGACAC TCTCCAGAAT GTAAACCTAC AAATGTCCCG AATTAAATCT 
GATCTACGAG TGACTCAGCA GGAAAAGGAA GCTTTAAAGC AAGAAGTGAT GTCTCTACAC 
AAGCAACTTC AGAATGCCAG TGACAAGAAC TGGGCCCCAG AAATAGGCAT CCATCCATCA 
GGATTCCATA ACCAACAGCA AAGGCTGTCC TGGGACAAGC TGGATCACCT GATAAATGAG 
GAACAACAGC TACTTTGGCA AGAGAATGAG AGAGTCCAAA CCATGGTACA GAACACCAAA 
CCGGAACTCA TACACTCCCG GGAGAAGGTC CGTCAGCTGG AATCCAACCT TCCTTCTCCT 
AAGCACCAAA AACATCTAAA TTCATCAGGT ACTGTGAAGC CCACAGAGCA GGAGAAGTTG 
AGCTTAAAGA GAGAATGTGA ACAGTTTCAA AAGGAGCGAT CTCCTACTAA TAGGAAGGTT 
AGTCAGATGA AATCCCTTGA ACGAGAATTA GAAACAATTC ATTTGGAAAA TGAAGGCCTG 
AAAAAGAAAC AAGTAAAACT GGATGAACAG CTAATGGAGA TGCAGCACCT GAGGTCCACT 
GTGATGCTTA GCCCATCCCC TCATGCTTGG GAACTGCAAC TGCTCCAGCA GCAAACCTGT 
CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG CTTCAACGTC AGCTGCTGCA GGCAGAAAGG 
ATAAACCAGT GCCTGCAGGA GGAACTTGAA AATAGGACTT CTGAAACCAA CACACCACAG 
GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT GCAGATTCCT ACAGAAGGAT TGGGCACCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_005211736.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211793.1 Bos taurus ninein (NIN), transcript variant X5, mRNA.

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CloneID	OBa06508
Clone ID Related Accession (Same CDS sequence)	XM_005211736.3 , XM_005193648.3 , XM_024997983.1 , XM_005211736.4
Accession Version	XM_005211736.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211736.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211793.1
CDS	216..6677
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_005211736.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211736.3

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_005193648.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005193705.1 Bos taurus ninein (NIN), transcript variant X5, mRNA.

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CloneID	OBa06508
Clone ID Related Accession (Same CDS sequence)	XM_005211736.3 , XM_005193648.3 , XM_024997983.1 , XM_005211736.4
Accession Version	XM_005193648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005193705.1
CDS	216..6677
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_005193648.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005193648.3

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_024997983.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853751.1 Bos taurus ninein (NIN), transcript variant X3, mRNA.

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CloneID	OBa06508
Clone ID Related Accession (Same CDS sequence)	XM_005211736.3 , XM_005193648.3 , XM_024997983.1 , XM_005211736.4
Accession Version	XM_024997983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853751.1
CDS	263..6724
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_024997983.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997983.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_005211736.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211793.1 Bos taurus ninein (NIN), transcript variant X2, mRNA.

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CloneID	OBa06508
Clone ID Related Accession (Same CDS sequence)	XM_005211736.3 , XM_005193648.3 , XM_024997983.1 , XM_005211736.4
Accession Version	XM_005211736.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211736.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211793.1
CDS	247..6708
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKAHLGDIPGSVPDNHSKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_005211736.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211736.4

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGCA CACCTTGGAG ATATTCCGGG TTCAGTTCCA 
GACAACCACA GTAAAGTTAG TCAGATGAAA TCCCTTGAAC GAGAATTAGA AACAATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAACAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGT GATGCTTAGC CCATCCCCTC ATGCTTGGGA ACTGCAACTG 
CTCCAGCAGC AAACCTGTCC AGTGGTTCCC AGGGAGCAGT TTCTGCAGCT TCAACGTCAG 
CTGCTGCAGG CAGAAAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA TAGGACTTCT 
GAAACCAACA CACCACAGGG AAACCAGGAA TACCTGGTAA CTGTCATGGA GGAACGAATG 
TTGGAAGTTG AACAGAAATT GAAACTGGTG AAAAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAATACTAAA GCAGATGCAA TAGTGAAGGA TTTGTATGTG 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTAGA GGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
ACACCAGCAC CACTGACTTC TACACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_003582709.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582757.1 Bos taurus ninein (NIN), transcript variant X6, mRNA.

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CloneID	OBa06509
Clone ID Related Accession (Same CDS sequence)	XM_003582709.4 , XM_003586563.4 , XM_024997982.1
Accession Version	XM_003582709.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582709.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GGAAACCAGG AATACCTGGT AACTGTCATG 
GAGGAACGAA TGTTGGAAGT TGAACAGAAA TTGAAACTGG TGAAAAGGCT TCTTCAGGAG 
AAAGTGAATC AGCTCAAAGA ACAACTCTGC AAGAATACTA AAGCAGATGC AATAGTGAAG 
GATTTGTATG TGGAAAATGC CCAGTTGTTG AAAGCTCTGG AAATGACTGA ACAGCGACAA 
AAAACAGCAG AGAAGAAAAA TTACCTCCTA GAGGAGAAGA TTGCCAGCCT CAGTAATATA 
GTCAGGAATC TGACACCAGC ACCACTGACT TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582757.1
CDS	215..6628
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_003582709.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582709.4

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GGAAACCAGG AATACCTGGT AACTGTCATG 
GAGGAACGAA TGTTGGAAGT TGAACAGAAA TTGAAACTGG TGAAAAGGCT TCTTCAGGAG 
AAAGTGAATC AGCTCAAAGA ACAACTCTGC AAGAATACTA AAGCAGATGC AATAGTGAAG 
GATTTGTATG TGGAAAATGC CCAGTTGTTG AAAGCTCTGG AAATGACTGA ACAGCGACAA 
AAAACAGCAG AGAAGAAAAA TTACCTCCTA GAGGAGAAGA TTGCCAGCCT CAGTAATATA 
GTCAGGAATC TGACACCAGC ACCACTGACT TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_003586563.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586611.1 Bos taurus ninein (NIN), transcript variant X6, mRNA.

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CloneID	OBa06509
Clone ID Related Accession (Same CDS sequence)	XM_003582709.4 , XM_003586563.4 , XM_024997982.1
Accession Version	XM_003586563.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586563.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GGAAACCAGG AATACCTGGT AACTGTCATG 
GAGGAACGAA TGTTGGAAGT TGAACAGAAA TTGAAACTGG TGAAAAGGCT TCTTCAGGAG 
AAAGTGAATC AGCTCAAAGA ACAACTCTGC AAGAATACTA AAGCAGATGC AATAGTGAAG 
GATTTGTATG TGGAAAATGC CCAGTTGTTG AAAGCTCTGG AAATGACTGA ACAGCGACAA 
AAAACAGCAG AGAAGAAAAA TTACCTCCTA GAGGAGAAGA TTGCCAGCCT CAGTAATATA 
GTCAGGAATC TGACACCAGC ACCACTGACT TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586611.1
CDS	215..6628
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_003586563.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586563.4

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GGAAACCAGG AATACCTGGT AACTGTCATG 
GAGGAACGAA TGTTGGAAGT TGAACAGAAA TTGAAACTGG TGAAAAGGCT TCTTCAGGAG 
AAAGTGAATC AGCTCAAAGA ACAACTCTGC AAGAATACTA AAGCAGATGC AATAGTGAAG 
GATTTGTATG TGGAAAATGC CCAGTTGTTG AAAGCTCTGG AAATGACTGA ACAGCGACAA 
AAAACAGCAG AGAAGAAAAA TTACCTCCTA GAGGAGAAGA TTGCCAGCCT CAGTAATATA 
GTCAGGAATC TGACACCAGC ACCACTGACT TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_024997982.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853750.1 Bos taurus ninein (NIN), transcript variant X1, mRNA.

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CloneID	OBa06509
Clone ID Related Accession (Same CDS sequence)	XM_003582709.4 , XM_003586563.4 , XM_024997982.1
Accession Version	XM_024997982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GGAAACCAGG AATACCTGGT AACTGTCATG 
GAGGAACGAA TGTTGGAAGT TGAACAGAAA TTGAAACTGG TGAAAAGGCT TCTTCAGGAG 
AAAGTGAATC AGCTCAAAGA ACAACTCTGC AAGAATACTA AAGCAGATGC AATAGTGAAG 
GATTTGTATG TGGAAAATGC CCAGTTGTTG AAAGCTCTGG AAATGACTGA ACAGCGACAA 
AAAACAGCAG AGAAGAAAAA TTACCTCCTA GAGGAGAAGA TTGCCAGCCT CAGTAATATA 
GTCAGGAATC TGACACCAGC ACCACTGACT TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853750.1
CDS	22..6435
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQGNQEYLVTVMEERMLEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAIVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLTPAPLTSTPPLRS

Target ORF information:

RefSeq Version	XM_024997982.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997982.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GGAAACCAGG AATACCTGGT AACTGTCATG 
GAGGAACGAA TGTTGGAAGT TGAACAGAAA TTGAAACTGG TGAAAAGGCT TCTTCAGGAG 
AAAGTGAATC AGCTCAAAGA ACAACTCTGC AAGAATACTA AAGCAGATGC AATAGTGAAG 
GATTTGTATG TGGAAAATGC CCAGTTGTTG AAAGCTCTGG AAATGACTGA ACAGCGACAA 
AAAACAGCAG AGAAGAAAAA TTACCTCCTA GAGGAGAAGA TTGCCAGCCT CAGTAATATA 
GTCAGGAATC TGACACCAGC ACCACTGACT TCTACACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_002700603.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700649.1 Bos taurus ninein (NIN), transcript variant X10, mRNA.

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CloneID	OBa06511
Clone ID Related Accession (Same CDS sequence)	XM_002700603.5 , XM_024997986.1 , XM_002690835.4
Accession Version	XM_002700603.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645878.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700603.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT 
GCAGATTCCT ACAGAAGGAT TGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700649.1
CDS	216..6368
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_002700603.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700603.5

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT 
GCAGATTCCT ACAGAAGGAT TGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_024997986.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853754.1 Bos taurus ninein (NIN), transcript variant X6, mRNA.

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CloneID	OBa06511
Clone ID Related Accession (Same CDS sequence)	XM_002700603.5 , XM_024997986.1 , XM_002690835.4
Accession Version	XM_024997986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ninein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT 
GCAGATTCCT ACAGAAGGAT TGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853754.1
CDS	22..6174
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_024997986.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997986.1

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT 
GCAGATTCCT ACAGAAGGAT TGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_002690835.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690881.1 Bos taurus ninein (NIN), transcript variant X10, mRNA.

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CloneID	OBa06511
Clone ID Related Accession (Same CDS sequence)	XM_002700603.5 , XM_024997986.1 , XM_002690835.4
Accession Version	XM_002690835.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ninein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT 
GCAGATTCCT ACAGAAGGAT TGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690881.1
CDS	216..6368
Translation	MDEAEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCTLEAQPKYVRGGKRYGRRSLPEFQESVEEFAEVTVIEPLGEEAQPSHIPASERSEHWKTQRSEEYEAEGQLRFWNPDDLKASQTGSLAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNVDGEMLEEVFHNLDPDGMMSVEDFFYGLFKNGKPLTPSASTPYRQLKRHISMQSFDESGRRTTAPSAMMSTIGFRVFSCLDDGMGYASVERILDTWQEEGIENSQEILKALDFSLDGNVNLTELTLALENELLVTKNSIHQAALASFKAEIRHLLERVDQMLREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNEFRKEREQILQQVGKQRLELEQEIEKAKTEENYVRDRLALSLKENSRLENELLENAEKLAEYESLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRLLQDQVDELQSELQEYRTQGKVSRLPLQNSLSEELDINSGCIEPDQGLGSEECNPLNMSIEAELVIEQMKEQHHRELCHLRLELEDKVNYYEKQLDETKVAFEKEQENMKLKCENEVHILEEQISDLKNKIAELQGQTEVLKEAQHVAICRHEEEKKQLQMKWDEEKAHVQEELRLEHEMALRARLEQVEESFNRERERLVQNGAWTEEKVRGLTQKLEQVHQEQLKSLVEKHILEKEELQKELLEKHQRELHEGREKMETECNRRTSQIEAQFQADCQKVTERYEHALRSLEVHYRHQLKELLDQQCEERSQWEFEKDELTQECAEAQEQLKETLQREKATSLVLTQEREMLEKTYKEHLNSMVVEREQLLQDLEDLRKVSESQQSLLSNQILELKSSHERELKDREQILCQAGTSEQLVSQRLEQLQREHDQERQEMMSKLLVMESVHRATCEKADRERAEMSTEISRLQNKIKEMEQVVSPPSRLQNSCQVIGGEEAEENGAMSLLQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQRLQKLEPESVMSSCLDEPATGLFGNSVEQTEPFLQPNRIKQAEGGTTQRILSDLQGDEVRDLESTGTSSGQRQVRVEESEASIESFSELENSEETRTETWDLKNQVGQLREQLMILRADCDRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKVLYEDASRENECLQEELRMMETRYDEALESNKELTSEMFRLQDELKKVEEMTDTFLSLEKSYKEIKRENEELHVLVLRLQGKIEKLRERAVLQCDCFSLWEAHLDNLEVGPDEKVFELNQTLDERVPNVMSVHHIIEEHLYQENQYLEQENTQLLEKVKAHEIAWLHGTLQTHRDKLGAQNQVVLEENTALLGLQDKHFQHQATIAELEREKKKLQELTRKLRERVTALVKQKDVPSQGEKEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLSGSQEEMWQKIETVKQEKAAVQKMVENLKKQISELKTKNQQLDLENMKLSQKNSQNQKELQELNQRLAEMLSQKDKEPGHSACEEWEQEKSNLKEELEHCKLQSSTLVSSLEAELSEVKIQTHIVEQENLLLKDELEKVKQLCRCPDLSDFQQKISSILSHNEKLLKEKEALSEELNICVDKLAKSSLLEHRIATMKQEQKSWEHQSESLKSQLVASQEKVQNLEDTLQNVNLQMSRIKSDLRVTQQEKEALKQEVMSLHKQLQNASDKNWAPEIGIHPSGFHNQQQRLSWDKLDHLINEEQQLLWQENERVQTMVQNTKPELIHSREKVRQLESNLPSPKHQKHLNSSGTVKPTEQEKLSLKRECEQFQKERSPTNRKVSQMKSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTVMLSPSPHAWELQLLQQQTCPVVPREQFLQLQRQLLQAERINQCLQEELENRTSETNTPQALLPEQRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_002690835.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ninein (NIN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690835.4

ORF Insert Sequence:

ATGGATGAGG CAGAGCAGGA CCAGCATGAG GCCCGACTCA AGGAGCTGTT TGACAGTTTT 
GACACGACGG GCACCGGGTC CCTGGGACAG GAGGAACTCA CCGACCTCTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC AGTGCTGCAG GAGACGCTGC TTCAGGACAA CCTCCTGGGC 
CGGGTGCATT TTGACCAGTT TAAAGAAGCA TTAATACTTA TCTTGTCTAG AACGCTATCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC ACTCTCGAAG CTCAGCCCAA ATATGTTAGA 
GGTGGGAAGC GTTATGGACG CAGATCTTTA CCTGAATTTC AAGAATCCGT GGAGGAATTT 
GCAGAGGTGA CTGTCATTGA GCCACTTGGT GAAGAAGCAC AGCCTTCACA CATCCCAGCC 
AGTGAGCGCA GCGAGCACTG GAAGACGCAG CGCAGCGAGG AGTATGAAGC AGAAGGCCAG 
TTAAGATTTT GGAACCCAGA TGATTTGAAA GCCTCACAGA CTGGGTCTTT GGCTCCCCAA 
GACTGGATAG AAGAGAAACT GCAGGAGGTT TGTGAAGATT TGGGGATCAC TCGTGATGGT 
CACCTAAACC GAAAGAAGCT GGTCTCCATC TGCGAGCAGT ATGGTTTGCA GAATGTCGAT 
GGAGAGATGC TTGAAGAAGT CTTCCATAAT CTTGATCCTG ATGGTATGAT GAGTGTAGAA 
GATTTTTTCT ATGGCTTGTT CAAAAATGGA AAACCTCTTA CACCATCAGC ATCTACCCCA 
TATAGGCAAT TGAAAAGGCA CATCTCCATG CAGTCTTTTG ATGAGAGTGG ACGACGGACC 
ACAGCCCCAT CTGCAATGAT GAGTACCATT GGTTTTCGGG TCTTCTCCTG CCTGGATGAT 
GGGATGGGCT ATGCATCAGT GGAGAGAATA CTTGACACCT GGCAGGAGGA AGGCATTGAA 
AACAGCCAGG AGATCCTGAA GGCCTTGGAT TTTAGCCTTG ATGGAAATGT CAACTTGACA 
GAGTTGACAC TGGCTCTTGA AAACGAACTT TTGGTCACCA AGAACAGCAT TCACCAGGCA 
GCTCTGGCCA GTTTTAAGGC TGAGATTCGG CATTTGCTGG AGCGAGTGGA TCAGATGCTC 
AGAGAAAAAG AGAAGCTACG GTCTGATCTG GACAAAGCTG AGAAGCTGAA GTCTCTAATG 
GCCTCAGAGG TGGACGATCA CCATGCAGCC ATAGAGCGGC GGAACGAGTA CAACCTCAGG 
AAACTGGATG AAGAGTACAA GGAGCGAATA GCAGCCTTAA AGAATGAGTT TCGAAAGGAG 
AGAGAGCAGA TCCTGCAGCA GGTGGGCAAG CAGCGTTTGG AACTTGAGCA AGAAATCGAA 
AAGGCAAAAA CAGAAGAGAA CTACGTCCGG GACCGCCTCG CACTCTCTTT AAAGGAAAAC 
AGTCGTCTAG AAAATGAGCT TTTGGAGAAT GCAGAGAAGT TGGCAGAGTA TGAGAGTCTG 
ACAAACAAAC TTCAGCGAAA TTTGGAAAAT GTTTTGGCAG AAAAGTTTGG TGACCTTGAT 
CCCAGCAGTG CTGAATTCTT CCTGCAAGAA GAAAGGCTGA CGCAGATGAG AAATGAATAT 
GAGCAGCAGT GCCGGCTATT ACAAGACCAA GTAGACGAAC TCCAATCTGA ACTGCAAGAA 
TATCGTACAC AAGGCAAAGT ATCCAGGCTT CCCTTGCAGA ACTCACTGTC AGAAGAACTT 
GATATTAACA GTGGTTGCAT AGAGCCTGAC CAGGGGCTTG GTTCTGAAGA ATGCAATCCA 
CTGAATATGA GCATCGAGGC AGAGCTGGTC ATCGAACAGA TGAAAGAACA ACATCACAGG 
GAGTTGTGTC ACCTAAGACT GGAGCTTGAA GATAAAGTGA ACTATTATGA AAAGCAGCTA 
GATGAAACCA AAGTTGCCTT TGAAAAGGAA CAGGAGAACA TGAAGCTCAA GTGTGAGAAT 
GAAGTACACA TCTTAGAAGA ACAAATAAGT GACCTTAAAA ATAAAATTGC AGAACTTCAG 
GGCCAGACAG AGGTACTCAA GGAGGCACAG CACGTGGCTA TCTGCAGACA TGAGGAGGAG 
AAAAAACAGC TGCAAATGAA GTGGGATGAG GAGAAGGCTC ACGTGCAGGA GGAGCTGCGG 
CTGGAGCATG AGATGGCACT CAGGGCTAGG CTGGAGCAGG TGGAGGAAAG CTTTAACAGA 
GAGAGGGAAA GACTCGTCCA AAATGGCGCC TGGACAGAGG AGAAGGTGAG GGGCTTGACT 
CAGAAACTAG AACAAGTTCA CCAGGAGCAG CTGAAAAGCC TGGTGGAGAA ACACATTCTT 
GAGAAAGAGG AATTGCAAAA AGAGCTCTTG GAAAAACACC AAAGGGAACT ACATGAGGGA 
AGGGAAAAAA TGGAAACTGA GTGTAATAGA AGAACCTCTC AGATAGAAGC CCAGTTTCAG 
GCTGATTGTC AGAAAGTCAC TGAGAGATAC GAACACGCCC TGCGGAGTCT GGAGGTGCAC 
TACCGCCACC AGCTGAAGGA GCTCCTGGAT CAACAATGTG AGGAGAGGTC CCAGTGGGAG 
TTTGAGAAGG ACGAGCTCAC CCAGGAATGT GCAGAAGCCC AGGAGCAGCT TAAAGAGACT 
CTCCAGAGGG AGAAAGCAAC CTCTTTGGTC CTCACCCAGG AGAGAGAGAT GCTGGAGAAA 
ACATACAAGG AGCATTTGAA TAGTATGGTT GTTGAGAGAG AGCAGCTACT ACAGGACCTG 
GAAGATCTGA GAAAAGTGTC TGAAAGTCAG CAAAGTCTCC TGTCCAACCA GATACTTGAG 
CTGAAGAGCA GTCATGAAAG AGAGCTGAAG GACCGTGAGC AGATCCTGTG CCAGGCAGGC 
ACTTCAGAGC AGCTGGTCAG CCAGAGGCTT GAACAGCTGC AAAGGGAACA TGACCAGGAG 
AGGCAGGAAA TGATGTCCAA GCTTCTGGTC ATGGAGAGTG TCCACAGAGC AACCTGTGAG 
AAGGCAGATC GAGAGAGGGC TGAGATGAGC ACAGAAATCT CCAGGCTCCA GAATAAAATC 
AAGGAGATGG AGCAGGTAGT GTCTCCTCCC TCCAGGCTTC AGAATAGCTG TCAAGTGATA 
GGAGGGGAGG AGGCAGAAGA AAATGGTGCC ATGTCCCTGC TTCAGCAAGG AGAACAGCTG 
TTGGAAGAGA ATGGAGATGT TCTCTTAAGC CTGCAAAGAG CCCATGAACG AGCAGTGAAG 
GAAAATGTGA AAATGGCAAC TGAAATTTCT AGGTTGCAGC AGAGGCTGCA AAAATTAGAA 
CCAGAGTCAG TAATGTCTTC TTGTTTAGAT GAACCAGCTA CTGGGCTCTT TGGAAATTCA 
GTGGAACAAA CAGAGCCATT TTTACAGCCA AACCGAATCA AACAAGCAGA AGGTGGAACC 
ACACAGCGTA TTCTCAGTGA CCTACAAGGC GATGAGGTCC GTGACCTCGA AAGTACAGGG 
ACGAGCTCTG GGCAGAGACA GGTCAGAGTG GAGGAGTCTG AAGCATCAAT AGAGAGTTTT 
TCCGAGCTTG AAAATAGTGA GGAGACCAGA ACTGAAACCT GGGACCTGAA GAATCAGGTC 
GGCCAGCTTC GGGAACAGTT AATGATCTTA CGTGCAGACT GCGATCGAGC GTCTGAAAAG 
AAACAGGACC TACTGTTTGA TGTTTCTGTG CTAAAAAAGA AACTGAAGAT GCTTGAAAGA 
ATTCCTGAGG CTTCCCCCAA GTATAAAGTG TTATACGAAG ATGCCAGTAG AGAAAATGAA 
TGTCTTCAGG AAGAGCTGAG GATGATGGAG ACGCGCTATG ATGAAGCACT AGAAAGTAAC 
AAAGAGCTCA CTTCAGAAAT GTTCAGATTA CAGGATGAGC TAAAGAAGGT TGAAGAAATG 
ACTGACACAT TCCTTAGCCT GGAAAAGAGT TACAAGGAGA TCAAAAGAGA AAATGAGGAA 
CTGCATGTTC TGGTTTTGAG ACTTCAAGGC AAGATTGAGA AGCTACGAGA AAGAGCAGTG 
CTGCAGTGTG ACTGCTTCTC CTTATGGGAA GCCCATTTAG ATAACCTGGA AGTCGGACCT 
GATGAAAAGG TCTTTGAACT GAACCAAACA CTGGATGAGC GTGTACCAAA TGTTATGAGC 
GTGCACCATA TTATAGAAGA ACATTTGTAT CAAGAAAACC AGTACCTAGA ACAGGAGAAT 
ACACAGCTCT TGGAGAAAGT AAAAGCACAT GAAATTGCCT GGCTACACGG AACACTCCAG 
ACACATCGAG ACAAGCTTGG AGCACAGAAC CAAGTTGTAC TGGAGGAAAA CACTGCTCTC 
CTCGGCCTTC AAGACAAGCA TTTTCAGCAC CAGGCCACCA TCGCAGAGTT AGAACGGGAG 
AAAAAAAAGC TGCAGGAGCT GACTAGAAAG TTGAGGGAAA GAGTCACTGC TTTAGTCAAG 
CAAAAGGATG TACCTTCTCA AGGAGAAAAG GAGGAAGAGC TGAAGGCAAT GATGCATGAC 
CTGCAAATCA CATGCAGTGA GATGCAGCAG AAAGTTGAGC TTCTGAGATA TGAATCTGAG 
AAGCTTCAAG AGGAAAATTC TATTTTGAGA AATGAAATTA CTACTTTAAA TGAAGAAGAC 
AGCATTTCTA ACCTGAAATT AGGGAAATTA AGTGGATCTC AGGAAGAAAT GTGGCAAAAA 
ATAGAAACTG TAAAACAGGA AAAAGCTGCA GTTCAGAAGA TGGTTGAAAA TTTAAAGAAA 
CAGATTTCAG AATTAAAAAC CAAAAACCAG CAGTTGGATT TGGAAAATAT GAAGCTTAGC 
CAAAAGAACT CTCAGAATCA GAAAGAGCTG CAAGAACTTA ATCAACGTCT GGCAGAAATG 
CTAAGCCAGA AGGACAAAGA GCCAGGGCAC AGTGCATGTG AGGAATGGGA ACAAGAAAAG 
TCTAATCTCA AAGAAGAACT GGAACACTGT AAATTGCAGT CTTCCACTTT AGTGTCTTCT 
TTGGAGGCAG AACTTTCTGA AGTTAAAATA CAGACTCACA TCGTGGAACA GGAAAACCTC 
CTTCTCAAAG ATGAATTGGA GAAAGTAAAA CAACTCTGCA GATGTCCTGA TCTCTCTGAC 
TTCCAGCAAA AAATTTCTAG TATTCTAAGC CACAATGAAA AACTGCTGAA AGAAAAGGAA 
GCTCTAAGTG AAGAATTAAA TATCTGTGTT GATAAGTTGG CAAAATCAAG TCTTTTAGAG 
CACAGAATTG CTACTATGAA GCAAGAACAG AAGTCTTGGG AACACCAGAG TGAAAGCTTA 
AAGTCACAAC TAGTGGCTTC ACAGGAAAAG GTTCAGAATT TAGAAGACAC TCTCCAGAAT 
GTAAACCTAC AAATGTCCCG AATTAAATCT GATCTACGAG TGACTCAGCA GGAAAAGGAA 
GCTTTAAAGC AAGAAGTGAT GTCTCTACAC AAGCAACTTC AGAATGCCAG TGACAAGAAC 
TGGGCCCCAG AAATAGGCAT CCATCCATCA GGATTCCATA ACCAACAGCA AAGGCTGTCC 
TGGGACAAGC TGGATCACCT GATAAATGAG GAACAACAGC TACTTTGGCA AGAGAATGAG 
AGAGTCCAAA CCATGGTACA GAACACCAAA CCGGAACTCA TACACTCCCG GGAGAAGGTC 
CGTCAGCTGG AATCCAACCT TCCTTCTCCT AAGCACCAAA AACATCTAAA TTCATCAGGT 
ACTGTGAAGC CCACAGAGCA GGAGAAGTTG AGCTTAAAGA GAGAATGTGA ACAGTTTCAA 
AAGGAGCGAT CTCCTACTAA TAGGAAGGTT AGTCAGATGA AATCCCTTGA ACGAGAATTA 
GAAACAATTC ATTTGGAAAA TGAAGGCCTG AAAAAGAAAC AAGTAAAACT GGATGAACAG 
CTAATGGAGA TGCAGCACCT GAGGTCCACT GTGATGCTTA GCCCATCCCC TCATGCTTGG 
GAACTGCAAC TGCTCCAGCA GCAAACCTGT CCAGTGGTTC CCAGGGAGCA GTTTCTGCAG 
CTTCAACGTC AGCTGCTGCA GGCAGAAAGG ATAAACCAGT GCCTGCAGGA GGAACTTGAA 
AATAGGACTT CTGAAACCAA CACACCACAG GCCTTGCTAC CGGAGCAGCG AGCAGTGCAT 
GCAGATTCCT ACAGAAGGAT TGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NIN
Entrez Gene ID	510366
Full Name	ninein
General protein information	Preferred Names ninein Names ninein ninein (GSK3B interacting protein)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	NIN	NP_065972.3
	NIN	XP_002805084.1
Gallus gallus (chicken)	NIN	XP_004936560.1
Rattus norvegicus (Norway rat)	Nin	NP_001100207.1
Rattus norvegicus (Norway rat)	LOC100911256	XP_006240209.1
Xenopus tropicalis (tropical clawed frog)	nin	XP_002936637.2
Pan troglodytes (chimpanzee)	NIN	XP_003314365.1
Canis lupus familiaris (dog)	NIN	XP_537442.2
Bos taurus (cattle)	NIN	XP_005211794.1
Mus musculus (house mouse)	Nin	NP_001074922.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NIN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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