ANKHD1 cDNA ORF clone, Bos taurus(cattle)

The following ANKHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236729 XM_024995219.1
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa236725 XM_024995216.1
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa236726 XM_024995217.1
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa236727 XM_005209497.4
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa236728 XM_024995218.1
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa21575 XM_005209497.3
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa21575 XM_015464301.1
Latest version!
Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236729
    Clone ID Related Accession (Same CDS sequence) XM_024995219.1
    Accession Version XM_024995219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and KH domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGAC AAGCAGGAGG ACATGAAGAC
    TATTTTGGAG GGCATAGATC CGGCCAAGCA TCAGGTGAGG GTGGCCTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850987.1
    CDS154..1884
    Translation

    Target ORF information:

    RefSeq Version XM_024995219.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGAC AAGCAGGAGG ACATGAAGAC
    TATTTTGGAG GGCATAGATC CGGCCAAGCA TCAGGTGAGG GTGGCCTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236725
    Clone ID Related Accession (Same CDS sequence) XM_024995216.1
    Accession Version XM_024995216.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and KH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAC AAGCAGGAGG ACATGAAGAC TATTTTGGAG
    GGCATAGATC CGGCCAAGCA TCAGGAGCAT GAATCTGAAG GTGGAAGAAC ACCTTTAATG
    AAAGCTGCAA GAGCTGGTCA TTTGTGTACT GTGCAGTTTC TTATTAGCAA AGGTGCTAAT
    GTGAATAGGG CTACAGCCAA TAATGATCAT ACAGTAGTGT CACTGGCATG TGCAGGAGGT
    CACCTGGCAG TGGTGGAGCT ACTCTTGGCT CATGGGGCTG ACCCTACTCA TCGTCTCAAG
    GATGGCTCAA CAATGCTCAT TGAAGCTGCA AAAGGTGGTC ATACTAATGT TGTTTCTTAT
    CTTTTGGATT ATCCAAACAA TGTTCTGTCA GTTCCTACCA CAGATGTGTC TCAGCTTACC
    CCACCTTCTC AAGATCAATC TCAGGTTCCA CGTGTGCCAA TGCATACACT TGCCATGGTT
    GTACCTCCTC AGGAACCTGA CAGAACTTTA CAGGAGAACT CTCCTGCTGT TTTAGGAGTA
    CAAAAAGGTA CATCCAAGCA GAAGTCCAGT TCCCTGCAGG TAGCAGATCA GGACGTACTG
    CCACCTTTTC ACCCATACCA GCCTTTGGAG TGCATAGTAG AGGAGACTGA AGGAAAACTG
    AATGAACTGG GACAAAGAAT TAGTGCTATT GAAAAAGCAC AGCTTAAATC ATTGGAGTTA
    ATTCAAGGTG AACCTCTGAA CAAAGATAAG ATAGAAGAAC TTAAAAAGAA CAGAGAAGAG
    CAAGTCCAGA AGAAGAAGAA AATACTGAAA GAACTGCAGA AAGTGGAAAG GCAGTTGCAG
    ATGAAAACAC AGCAGCAATT TACCAAAGAA TATCTGGAAA CCAAAGGTCA GAAAGACACA
    GTATCTCTAC ACCAGCAGTG CTCTCATAGA GGAGTCTTCC CAGAAGGGGA AGGAGATGGT
    AGTCTCCCAG AGGATCACTT TTCAGAGTTA CCTCAGGTTG ACACAATCTT ATTTAAAGAT
    AATGATGTTG ATGATGAGCA ACAGTCTCCA CCATCGGCAG AACAAATTGA TTTTGTCCCA
    GTCCAGCCTT TATCATCTCC ACAGTGTAAC TTTTCCAGTG AATTAGGTTC TAATGGGACA
    AATTCTCTTG AACTTCAGAA AGTATCAGGT AATCATCAGA TTATAGGACA GCCTCAGATT
    GCTATTACTG GACATGATCA GGGGCTGTTA GTGCAAGAAC CAGATGGACT AATGGTTGCA
    ACTCCAGCCC AGACGCTTAC CGACACTCTT GATGACCTGA TAGCAGCTGT GAGTAGCAGA
    GTGCCCACTG GCTCAAACAG CTCTTCTCAA AACGTAGAAT GTCTTACACC TGAACCCTGT
    TCTCAGTCTC CAAGCAATGT GGCTTCTCAG TCTATACCCC CTGTGTATCC TTCAGTTGAC
    ATTGATGCAC ATACTGAGAG CAATCATGAC ACTGCATTAA CCCTAGCTTG TGCAGGTGGC
    CATGAAGAAC TTGTATCTGT ACTCATAGCA CGAGATGCTA AAATTGAACA CAGAGACAAA
    AAAGGTTTCA CACCACTGAT CCTGGCAGCA ACAGCAGGAC ATGTTGGAGT TGTTGAAATC
    CTTTTGGATA AAGGTGGAGA CATAGAAGCA CAATCTGAAC GAACTAAGGA TACTCCTCTT
    TCATTAGCAT GTTCTGGTGG ACGTCAGGAA GTGGTAGACT TGCTGTTGGC TCGGGGTGCA
    AATAAAGAAC ATAGGAACGT GTCTGATTAT ACACCGCTGA GTCTAGCTGC ATCTGGAGGA
    TATGTTAATA TCATTAAGAT TCTGCTTAAT GCTGGGGCAG AAATTAATTC AAGGACTGGG
    AGTAAACTAG GTATTTCTCC CCTGATGTTG GCTGCAATGA ATGGACATGT TCCTGCAGTG
    AAACTGCTGC TTGATATGGG TTCAGATATT AATGCCCAAA TAGAGACCAA TCGGAACACA
    GCTCTCACCT TGGCCTGTTT CCAAGGCCGA GCGGAAGTAG TGAGCTTGCT TCTGGACCGA
    AAAGCCAATG TTGAACATAG GGCAAAGACT GGTCTTACCC CCTTGATGGA AGCTGCTTCT
    GGGGGATATG CAGAGGTTGG AAGAGTTCTC CTAGATAAAG GAGCAGATGT CAATGCCCCC
    CCTGTGCCTT CCTCAAGAGA CACTGCTTTA ACAATAGCAG CGGACAAAGG TCACTACAAA
    TTTTGTGAGC TCTTGATTAA TAGGGGAGCC CATATTGATG TTCGTAACAA GAAGGGAAAC
    ACACCACTTT GGTTGGCATC CAATGGTGGT CATTTTGAAG TGGTGCAGTT GCTAGTGCGA
    TCAGGTGCTG ATGTGGATGG AGCTGATAAC CGGAAAATCA CACCTCTTAT GTCAGCATTT
    CGAAAGGGTC ATGTAAAAGT TGTTCAGTAT TTGGTTAAGG AAGTCAATCA GTTCCCTTCT
    GATATAGAAT GCATGAGATA CATAGCAACA ATCACTGATA AGGAACTGTT GAAAAAATGT
    CACCAATGTG TTGAGACAAT TGTGAAGGCT AAAGACCAAC AGGCTGCAGA AGCAAATAAG
    AATGCAAGTA TTCTTTTAAA GGAACTTGAT CTGGAAAAGT CAAGAGAGGA GAGCAGAAAG
    CAAGCTCTTG CAGCTAAAAG AGAGAAAAGA AAAGAAAAGA GAAAGAAGAA AAAAGAGGAG
    CAGAAAAGAA AGCAGGAAGA AGACGAGGAA AACAAACCTA AGGAGAATTC AGAACTACCG
    GAAGATGAAG ATGAAGAGGA GAATGAGGAA GATGTGGAGC AAGAAGTTCC CATAGAACCT
    CCTAGTGCGA CTACCACCAC CACAATTGGA ATCTCTGCAA CATCTGCAAC ATTCACAAAT
    GTGTTTGGGA AAAAAAGGGC CAATGTTGTG ACAACTCCTA GCACCAACCG CAAAAATAAG
    AAGAACAAAA CCAAAGAGAC TCCTCCCACT GCACATTTAA TATTACCAGA ACAACATATA
    TCCTTAGCAC AGCAAAAGGC AGATAAGAAT AAAATAAACG GAGAAAGCAA AGGTGGTGGT
    GCAGGTGGGA ATAGTGATTC AGATAACTTG GACAGCACAG ATTGCAACAG TGAGAGTAGC
    AGTGGTGGTA AAAGCCAAGA ATTAAATTTC ACTATGGATG TGAATTCTTC TAGCGATAGG
    AGATACCCCC TACTGCTACT CCATTCCCAG GAGGAAAAGA CAAGTACCGC TGCTTCCAAA
    ACTCCAACAC GACTTGAAGG CGAAGTGAAT CCTAATTCCT TATCAACAAC CTACAAGTCA
    GTGTCATTGC CATTAAGTTC TCCAAACATA AAGCTGAATC TGACTAGCCC TAAAAGGGGG
    CAAAAAAGAG AGGAAGGATG GAAAGAAGTT GTCCGAAGGT CAAAAAAATT ATCTGTTCCA
    GCCTCAGTGG TTTCCAGAAT AATGGGAAGA GGAGGATGCA ACATCACTGC AATTCAAGAT
    GTTACTGGTG CCCACATTGA TGTGGATAAA CAAAAAGATA AGAATGGCGA GAGAATGATC
    ACAATTAGGG GTGGTACAGA ATCAACAAGA TCTGCAGTTC AACTTATTAA TGCACTTATT
    CAAGATCCTG CTAAGGAACT GGAAGACTTG ATTCCTAAAA ACCATATCAG AACACCTGCC
    AGCATCAAAG CCATTCATGC AAACTTCTCA TCTGGAGTAG GCACCACAGC AGCTTCCAGT
    AAAAATGCAT TTCCTTTGGG TGCTCCAAGT CTGGTAACTT CACAGGCAAC AACATTATCT
    ACGTTCCAGC CCACTAATAA ACTTAATAAG AACGTTCCAG CAAACGTACG TTCTTCTTTC
    CCAGTTTCTC TGCCCTTAGC TTATCCCCAC CCGCATTTTG CCCTGTTGGC TGCTCAAACC
    ATGCAACAGA TTCGGCATCC TCGCTTACCC ATGGCCCAGT TTGGAGGAAC CTTCTCACCC
    TCTCCTAACA CTTGGGGACC ATTCCCAGTG AGACCTGTGA ATCCTGGCAA CACAAACAGC
    TCTCCAAAGC ATAATAATAC AGGCCGTCTA CCTAACCAGA ACGGAACTGT TCTACCGTCA
    GAGTCTACTG GACTAGCTAC TGCCAGTTGC CCTATCACTG TCTCTTCTGT AGTTGCTGCC
    AGTCAGCAAC TGTGTGTGAC TAATACCCGG ACTCCTTCAT CAGTCAGAAA GCAGCTGTTT
    GCCTGTGTGC CTAAGACAAG CCCTCCAGCA ACAGTGATTA CTTCTATGAC AAGCACTTGT
    AGTTCCCTGC CTTCAGTCTC CTCTGCACCT ATCACTAGTG GGCAAGTTCC CACCACATTT
    CTGCCTGCAA GTACTTCTCA AGCACAGCTT TCTTCACAAA AGATGGAGTC TTTCTCTGCC
    ATGCCCGCCC CCAAAGAGAA ACTGTCCACA CAGGACCAGT CCACGGCAAA CCTGTGTCCC
    CCCTCTTCAA CTGCCAACAG CTGCAGTAGC TCTGCCAGCA ACACCCCAGG AGCTCCAGAA
    ACTCACCCAT CTGGTAGTCC CACTCCTCTG TCCAATAACA CACAGGAGGA AGCACAGCCA
    TCCAGTGTGT CTGATTTAAG TCCTATGTCA ATGCCTTTTG CATCTAATTC AGAACCTGCT
    CCATTGACTT TAGCATCACC TAGATTGGTT GCTGCTGATA ATCAGGATAC CAGTAATTTA
    CCTCAGTTAG CTGTACCAGC ACCTCGAGTT TCTCATCGAT TGCAACCCAG AGGTTCTTTC
    TACTCAGTGG TACCAAATGC AACTATACAC CAGGATCCCC AGTCCATTTT TGTTACAAAT
    CCAGTTCCTT TAACACCACC TCAAGGCCCA CCAGCTACAG TGCAGCTTTC TTCAGCCATG
    AACATTATGA ATGGTTCTCA GATGCACATT AACCCAGCAA ATAAATCTTT GCCACCTACG
    TTTGGCCCGG CCACGCTTTT CAATCACTTC AGTAGTCTCT TTGATAGCAG TCAGGTGCCA
    GCTAACCAGG GTTGGGGAGA TGGTCCTCTG TCCTCACGAG TTGCTGCAGA TGCCTCTTTC
    ACTGTTCAGT CAGCATTTCT GGGTAGCTCT GTACTTGGAC ACTTGGAGAA CGTGCACCCT
    GACAATTCCA AGGCACCTGG CTTCAGATCA CCTTCCCAGC GAGTTTCTAC TAGTCCAGTT
    GGATTACCGT CCATTGACCC ATCAGGCAGC TCCCCATCTT CCTCTTCTGC TCCTCTGACA
    AGTTTTTCCA GCATACCAGG AACTCGGGTT TTCCTGCAAG GGCCAGCTCC TGTTGGGACC
    CCTAGTTTCA ACAGACAACA TTTTTCTCCC CACCCTTGGA CGAGCGCCTC AAACTCATGT
    GACTCTCCTA TTCCATCTGT TTCTTCGGGA TCATCTTCAC CTCTTTCAGC CACTTCTGCC
    CCACCAACAT TGGGCCAACC AAAAGGAGGC AGTGCCAGTC AGGATCGAAA AATCCCTCCC
    CCCATTGGAA CAGAGAGACT AGCCCGAATT CGACAAGGAG GGTCTGTCGC ACAAGCCCCT
    GTGGGAACCG GTTTTGTCGC TCCTGTTGGA CACAGTGGAA TCTGGTCATT TGGTGTCAAT
    GCCATGTCAG AAGGGTTATC AGGTTGGTCA CAGTCTGTGA TAGGGAACCA TCCAATGCAT
    CAACAATTGT CAGACCCAAG CACATTCTCT CAGCATCAGC CAATGGAGAG AGATGATTCT
    GGAATGGTAG CCCCCTCTAA CATTTTTCAT CAGCCTATGG CAAGCAGTTT TGTGGATTTT
    TCTAAAGGTC TACCAATTTC CATGTATGGA GGCACCATAA TACCCTCCCA TCCTCAGCTT
    GCTGATGTTC CAGGAGGCCC TCTGTTTAAT GGACTTCACA ACCCAGATCC TTCTTGGAAC
    CCTATGATAA AAGTTATTCA AAATTCAACT GAATGCACTG ATGCCCAGCA GATTTGGCCT
    GGCACGTGGG CACCTCATAT TGGAAACATG CATCTCAAAT ATGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850984.1
    CDS148..7878
    Translation

    Target ORF information:

    RefSeq Version XM_024995216.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995216.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAC AAGCAGGAGG ACATGAAGAC TATTTTGGAG
    GGCATAGATC CGGCCAAGCA TCAGGAGCAT GAATCTGAAG GTGGAAGAAC ACCTTTAATG
    AAAGCTGCAA GAGCTGGTCA TTTGTGTACT GTGCAGTTTC TTATTAGCAA AGGTGCTAAT
    GTGAATAGGG CTACAGCCAA TAATGATCAT ACAGTAGTGT CACTGGCATG TGCAGGAGGT
    CACCTGGCAG TGGTGGAGCT ACTCTTGGCT CATGGGGCTG ACCCTACTCA TCGTCTCAAG
    GATGGCTCAA CAATGCTCAT TGAAGCTGCA AAAGGTGGTC ATACTAATGT TGTTTCTTAT
    CTTTTGGATT ATCCAAACAA TGTTCTGTCA GTTCCTACCA CAGATGTGTC TCAGCTTACC
    CCACCTTCTC AAGATCAATC TCAGGTTCCA CGTGTGCCAA TGCATACACT TGCCATGGTT
    GTACCTCCTC AGGAACCTGA CAGAACTTTA CAGGAGAACT CTCCTGCTGT TTTAGGAGTA
    CAAAAAGGTA CATCCAAGCA GAAGTCCAGT TCCCTGCAGG TAGCAGATCA GGACGTACTG
    CCACCTTTTC ACCCATACCA GCCTTTGGAG TGCATAGTAG AGGAGACTGA AGGAAAACTG
    AATGAACTGG GACAAAGAAT TAGTGCTATT GAAAAAGCAC AGCTTAAATC ATTGGAGTTA
    ATTCAAGGTG AACCTCTGAA CAAAGATAAG ATAGAAGAAC TTAAAAAGAA CAGAGAAGAG
    CAAGTCCAGA AGAAGAAGAA AATACTGAAA GAACTGCAGA AAGTGGAAAG GCAGTTGCAG
    ATGAAAACAC AGCAGCAATT TACCAAAGAA TATCTGGAAA CCAAAGGTCA GAAAGACACA
    GTATCTCTAC ACCAGCAGTG CTCTCATAGA GGAGTCTTCC CAGAAGGGGA AGGAGATGGT
    AGTCTCCCAG AGGATCACTT TTCAGAGTTA CCTCAGGTTG ACACAATCTT ATTTAAAGAT
    AATGATGTTG ATGATGAGCA ACAGTCTCCA CCATCGGCAG AACAAATTGA TTTTGTCCCA
    GTCCAGCCTT TATCATCTCC ACAGTGTAAC TTTTCCAGTG AATTAGGTTC TAATGGGACA
    AATTCTCTTG AACTTCAGAA AGTATCAGGT AATCATCAGA TTATAGGACA GCCTCAGATT
    GCTATTACTG GACATGATCA GGGGCTGTTA GTGCAAGAAC CAGATGGACT AATGGTTGCA
    ACTCCAGCCC AGACGCTTAC CGACACTCTT GATGACCTGA TAGCAGCTGT GAGTAGCAGA
    GTGCCCACTG GCTCAAACAG CTCTTCTCAA AACGTAGAAT GTCTTACACC TGAACCCTGT
    TCTCAGTCTC CAAGCAATGT GGCTTCTCAG TCTATACCCC CTGTGTATCC TTCAGTTGAC
    ATTGATGCAC ATACTGAGAG CAATCATGAC ACTGCATTAA CCCTAGCTTG TGCAGGTGGC
    CATGAAGAAC TTGTATCTGT ACTCATAGCA CGAGATGCTA AAATTGAACA CAGAGACAAA
    AAAGGTTTCA CACCACTGAT CCTGGCAGCA ACAGCAGGAC ATGTTGGAGT TGTTGAAATC
    CTTTTGGATA AAGGTGGAGA CATAGAAGCA CAATCTGAAC GAACTAAGGA TACTCCTCTT
    TCATTAGCAT GTTCTGGTGG ACGTCAGGAA GTGGTAGACT TGCTGTTGGC TCGGGGTGCA
    AATAAAGAAC ATAGGAACGT GTCTGATTAT ACACCGCTGA GTCTAGCTGC ATCTGGAGGA
    TATGTTAATA TCATTAAGAT TCTGCTTAAT GCTGGGGCAG AAATTAATTC AAGGACTGGG
    AGTAAACTAG GTATTTCTCC CCTGATGTTG GCTGCAATGA ATGGACATGT TCCTGCAGTG
    AAACTGCTGC TTGATATGGG TTCAGATATT AATGCCCAAA TAGAGACCAA TCGGAACACA
    GCTCTCACCT TGGCCTGTTT CCAAGGCCGA GCGGAAGTAG TGAGCTTGCT TCTGGACCGA
    AAAGCCAATG TTGAACATAG GGCAAAGACT GGTCTTACCC CCTTGATGGA AGCTGCTTCT
    GGGGGATATG CAGAGGTTGG AAGAGTTCTC CTAGATAAAG GAGCAGATGT CAATGCCCCC
    CCTGTGCCTT CCTCAAGAGA CACTGCTTTA ACAATAGCAG CGGACAAAGG TCACTACAAA
    TTTTGTGAGC TCTTGATTAA TAGGGGAGCC CATATTGATG TTCGTAACAA GAAGGGAAAC
    ACACCACTTT GGTTGGCATC CAATGGTGGT CATTTTGAAG TGGTGCAGTT GCTAGTGCGA
    TCAGGTGCTG ATGTGGATGG AGCTGATAAC CGGAAAATCA CACCTCTTAT GTCAGCATTT
    CGAAAGGGTC ATGTAAAAGT TGTTCAGTAT TTGGTTAAGG AAGTCAATCA GTTCCCTTCT
    GATATAGAAT GCATGAGATA CATAGCAACA ATCACTGATA AGGAACTGTT GAAAAAATGT
    CACCAATGTG TTGAGACAAT TGTGAAGGCT AAAGACCAAC AGGCTGCAGA AGCAAATAAG
    AATGCAAGTA TTCTTTTAAA GGAACTTGAT CTGGAAAAGT CAAGAGAGGA GAGCAGAAAG
    CAAGCTCTTG CAGCTAAAAG AGAGAAAAGA AAAGAAAAGA GAAAGAAGAA AAAAGAGGAG
    CAGAAAAGAA AGCAGGAAGA AGACGAGGAA AACAAACCTA AGGAGAATTC AGAACTACCG
    GAAGATGAAG ATGAAGAGGA GAATGAGGAA GATGTGGAGC AAGAAGTTCC CATAGAACCT
    CCTAGTGCGA CTACCACCAC CACAATTGGA ATCTCTGCAA CATCTGCAAC ATTCACAAAT
    GTGTTTGGGA AAAAAAGGGC CAATGTTGTG ACAACTCCTA GCACCAACCG CAAAAATAAG
    AAGAACAAAA CCAAAGAGAC TCCTCCCACT GCACATTTAA TATTACCAGA ACAACATATA
    TCCTTAGCAC AGCAAAAGGC AGATAAGAAT AAAATAAACG GAGAAAGCAA AGGTGGTGGT
    GCAGGTGGGA ATAGTGATTC AGATAACTTG GACAGCACAG ATTGCAACAG TGAGAGTAGC
    AGTGGTGGTA AAAGCCAAGA ATTAAATTTC ACTATGGATG TGAATTCTTC TAGCGATAGG
    AGATACCCCC TACTGCTACT CCATTCCCAG GAGGAAAAGA CAAGTACCGC TGCTTCCAAA
    ACTCCAACAC GACTTGAAGG CGAAGTGAAT CCTAATTCCT TATCAACAAC CTACAAGTCA
    GTGTCATTGC CATTAAGTTC TCCAAACATA AAGCTGAATC TGACTAGCCC TAAAAGGGGG
    CAAAAAAGAG AGGAAGGATG GAAAGAAGTT GTCCGAAGGT CAAAAAAATT ATCTGTTCCA
    GCCTCAGTGG TTTCCAGAAT AATGGGAAGA GGAGGATGCA ACATCACTGC AATTCAAGAT
    GTTACTGGTG CCCACATTGA TGTGGATAAA CAAAAAGATA AGAATGGCGA GAGAATGATC
    ACAATTAGGG GTGGTACAGA ATCAACAAGA TCTGCAGTTC AACTTATTAA TGCACTTATT
    CAAGATCCTG CTAAGGAACT GGAAGACTTG ATTCCTAAAA ACCATATCAG AACACCTGCC
    AGCATCAAAG CCATTCATGC AAACTTCTCA TCTGGAGTAG GCACCACAGC AGCTTCCAGT
    AAAAATGCAT TTCCTTTGGG TGCTCCAAGT CTGGTAACTT CACAGGCAAC AACATTATCT
    ACGTTCCAGC CCACTAATAA ACTTAATAAG AACGTTCCAG CAAACGTACG TTCTTCTTTC
    CCAGTTTCTC TGCCCTTAGC TTATCCCCAC CCGCATTTTG CCCTGTTGGC TGCTCAAACC
    ATGCAACAGA TTCGGCATCC TCGCTTACCC ATGGCCCAGT TTGGAGGAAC CTTCTCACCC
    TCTCCTAACA CTTGGGGACC ATTCCCAGTG AGACCTGTGA ATCCTGGCAA CACAAACAGC
    TCTCCAAAGC ATAATAATAC AGGCCGTCTA CCTAACCAGA ACGGAACTGT TCTACCGTCA
    GAGTCTACTG GACTAGCTAC TGCCAGTTGC CCTATCACTG TCTCTTCTGT AGTTGCTGCC
    AGTCAGCAAC TGTGTGTGAC TAATACCCGG ACTCCTTCAT CAGTCAGAAA GCAGCTGTTT
    GCCTGTGTGC CTAAGACAAG CCCTCCAGCA ACAGTGATTA CTTCTATGAC AAGCACTTGT
    AGTTCCCTGC CTTCAGTCTC CTCTGCACCT ATCACTAGTG GGCAAGTTCC CACCACATTT
    CTGCCTGCAA GTACTTCTCA AGCACAGCTT TCTTCACAAA AGATGGAGTC TTTCTCTGCC
    ATGCCCGCCC CCAAAGAGAA ACTGTCCACA CAGGACCAGT CCACGGCAAA CCTGTGTCCC
    CCCTCTTCAA CTGCCAACAG CTGCAGTAGC TCTGCCAGCA ACACCCCAGG AGCTCCAGAA
    ACTCACCCAT CTGGTAGTCC CACTCCTCTG TCCAATAACA CACAGGAGGA AGCACAGCCA
    TCCAGTGTGT CTGATTTAAG TCCTATGTCA ATGCCTTTTG CATCTAATTC AGAACCTGCT
    CCATTGACTT TAGCATCACC TAGATTGGTT GCTGCTGATA ATCAGGATAC CAGTAATTTA
    CCTCAGTTAG CTGTACCAGC ACCTCGAGTT TCTCATCGAT TGCAACCCAG AGGTTCTTTC
    TACTCAGTGG TACCAAATGC AACTATACAC CAGGATCCCC AGTCCATTTT TGTTACAAAT
    CCAGTTCCTT TAACACCACC TCAAGGCCCA CCAGCTACAG TGCAGCTTTC TTCAGCCATG
    AACATTATGA ATGGTTCTCA GATGCACATT AACCCAGCAA ATAAATCTTT GCCACCTACG
    TTTGGCCCGG CCACGCTTTT CAATCACTTC AGTAGTCTCT TTGATAGCAG TCAGGTGCCA
    GCTAACCAGG GTTGGGGAGA TGGTCCTCTG TCCTCACGAG TTGCTGCAGA TGCCTCTTTC
    ACTGTTCAGT CAGCATTTCT GGGTAGCTCT GTACTTGGAC ACTTGGAGAA CGTGCACCCT
    GACAATTCCA AGGCACCTGG CTTCAGATCA CCTTCCCAGC GAGTTTCTAC TAGTCCAGTT
    GGATTACCGT CCATTGACCC ATCAGGCAGC TCCCCATCTT CCTCTTCTGC TCCTCTGACA
    AGTTTTTCCA GCATACCAGG AACTCGGGTT TTCCTGCAAG GGCCAGCTCC TGTTGGGACC
    CCTAGTTTCA ACAGACAACA TTTTTCTCCC CACCCTTGGA CGAGCGCCTC AAACTCATGT
    GACTCTCCTA TTCCATCTGT TTCTTCGGGA TCATCTTCAC CTCTTTCAGC CACTTCTGCC
    CCACCAACAT TGGGCCAACC AAAAGGAGGC AGTGCCAGTC AGGATCGAAA AATCCCTCCC
    CCCATTGGAA CAGAGAGACT AGCCCGAATT CGACAAGGAG GGTCTGTCGC ACAAGCCCCT
    GTGGGAACCG GTTTTGTCGC TCCTGTTGGA CACAGTGGAA TCTGGTCATT TGGTGTCAAT
    GCCATGTCAG AAGGGTTATC AGGTTGGTCA CAGTCTGTGA TAGGGAACCA TCCAATGCAT
    CAACAATTGT CAGACCCAAG CACATTCTCT CAGCATCAGC CAATGGAGAG AGATGATTCT
    GGAATGGTAG CCCCCTCTAA CATTTTTCAT CAGCCTATGG CAAGCAGTTT TGTGGATTTT
    TCTAAAGGTC TACCAATTTC CATGTATGGA GGCACCATAA TACCCTCCCA TCCTCAGCTT
    GCTGATGTTC CAGGAGGCCC TCTGTTTAAT GGACTTCACA ACCCAGATCC TTCTTGGAAC
    CCTATGATAA AAGTTATTCA AAATTCAACT GAATGCACTG ATGCCCAGCA GATTTGGCCT
    GGCACGTGGG CACCTCATAT TGGAAACATG CATCTCAAAT ATGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236726
    Clone ID Related Accession (Same CDS sequence) XM_024995217.1
    Accession Version XM_024995217.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and KH domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAC AAGCAGGAGG ACATGAAGAC TATTTTGGAG
    GGCATAGATC CGGCCAAGCA TCAGGAGCAT GAATCTGAAG GTGGAAGAAC ACCTTTAATG
    AAAGCTGCAA GAGCTGGTCA TTTGTGTACT GTGCAGTTTC TTATTAGCAA AGGTGCTAAT
    GTGAATAGGG CTACAGCCAA TAATGATCAT ACAGTAGTGT CACTGGCATG TGCAGGAGGT
    CACCTGGCAG TGGTGGAGCT ACTCTTGGCT CATGGGGCTG ACCCTACTCA TCGTCTCAAG
    GATGGCTCAA CAATGCTCAT TGAAGCTGCA AAAGGTGGTC ATACTAATGT TGTTTCTTAT
    CTTTTGGATT ATCCAAACAA TGTTCTGTCA GTTCCTACCA CAGATGTGTC TCAGCTTACC
    CCACCTTCTC AAGATCAATC TCAGGTTCCA CGTGTGCCAA TGCATACACT TGCCATGGTT
    GTACCTCCTC AGGAACCTGA CAGAACTTTA CAGGAGAACT CTCCTGCTGT TTTAGGAGTA
    CAAAAAGGTA CATCCAAGCA GAAGTCCAGT TCCCTGCAGG TAGCAGATCA GGACGTACTG
    CCACCTTTTC ACCCATACCA GCCTTTGGAG TGCATAGTAG AGGAGACTGA AGGAAAACTG
    AATGAACTGG GACAAAGAAT TAGTGCTATT GAAAAAGCAC AGCTTAAATC ATTGGAGTTA
    ATTCAAGGTG AACCTCTGAA CAAAGATAAG ATAGAAGAAC TTAAAAAGAA CAGAGAAGAG
    CAAGTCCAGA AGAAGAAGAA AATACTGAAA GAACTGCAGA AAGTGGAAAG GCAGTTGCAG
    ATGAAAACAC AGCAGCAATT TACCAAAGAA TATCTGGAAA CCAAAGGTCA GAAAGACACA
    GTATCTCTAC ACCAGCAGTG CTCTCATAGA GGAGTCTTCC CAGAAGGGGA AGGAGATGGT
    AGTCTCCCAG AGGATCACTT TTCAGAGTTA CCTCAGGTTG ACACAATCTT ATTTAAAGAT
    AATGATGTTG ATGATGAGCA ACAGTCTCCA CCATCGGCAG AACAAATTGA TTTTGTCCCA
    GTCCAGCCTT TATCATCTCC ACAGTGTAAC TTTTCCAGTG AATTAGGTTC TAATGGGACA
    AATTCTCTTG AACTTCAGAA AGTATCAGGT AATCATCAGA TTATAGGACA GCCTCAGATT
    GCTATTACTG GACATGATCA GGGGCTGTTA GTGCAAGAAC CAGATGGACT AATGGTTGCA
    ACTCCAGCCC AGACGCTTAC CGACACTCTT GATGACCTGA TAGCAGCTGT GAGTAGCAGA
    GTGCCCACTG GCTCAAACAG CTCTTCTCAA AACGTAGAAT GTCTTACACC TGAACCCTGT
    TCTCAGTCTC CAAGCAATGT GGCTTCTCAG TCTATACCCC CTGTGTATCC TTCAGTTGAC
    ATTGATGCAC ATACTGAGAG CAATCATGAC ACTGCATTAA CCCTAGCTTG TGCAGGTGGC
    CATGAAGAAC TTGTATCTGT ACTCATAGCA CGAGATGCTA AAATTGAACA CAGAGACAAA
    AAAGGTTTCA CACCACTGAT CCTGGCAGCA ACAGCAGGAC ATGTTGGAGT TGTTGAAATC
    CTTTTGGATA AAGGTGGAGA CATAGAAGCA CAATCTGAAC GAACTAAGGA TACTCCTCTT
    TCATTAGCAT GTTCTGGTGG ACGTCAGGAA GTGGTAGACT TGCTGTTGGC TCGGGGTGCA
    AATAAAGAAC ATAGGAACGT GTCTGATTAT ACACCGCTGA GTCTAGCTGC ATCTGGAGGA
    TATGTTAATA TCATTAAGAT TCTGCTTAAT GCTGGGGCAG AAATTAATTC AAGGACTGGG
    AGTAAACTAG GTATTTCTCC CCTGATGTTG GCTGCAATGA ATGGACATGT TCCTGCAGTG
    AAACTGCTGC TTGATATGGG TTCAGATATT AATGCCCAAA TAGAGACCAA TCGGAACACA
    GCTCTCACCT TGGCCTGTTT CCAAGGCCGA GCGGAAGTAG TGAGCTTGCT TCTGGACCGA
    AAAGCCAATG TTGAACATAG GGCAAAGACT GGTCTTACCC CCTTGATGGA AGCTGCTTCT
    GGGGGATATG CAGAGGTTGG AAGAGTTCTC CTAGATAAAG GAGCAGATGT CAATGCCCCC
    CCTGTGCCTT CCTCAAGAGA CACTGCTTTA ACAATAGCAG CGGACAAAGG TCACTACAAA
    TTTTGTGAGC TCTTGATTAA TAGGGGAGCC CATATTGATG TTCGTAACAA GAAGGGAAAC
    ACACCACTTT GGTTGGCATC CAATGGTGGT CATTTTGAAG TGGTGCAGTT GCTAGTGCGA
    TCAGGTGCTG ATGTGGATGG AGCTGATAAC CGGAAAATCA CACCTCTTAT GTCAGCATTT
    CGAAAGGGTC ATGTAAAAGT TGTTCAGTAT TTGGTTAAGG AAGTCAATCA GTTCCCTTCT
    GATATAGAAT GCATGAGATA CATAGCAACA ATCACTGATA AGGAACTGTT GAAAAAATGT
    CACCAATGTG TTGAGACAAT TGTGAAGGCT AAAGACCAAC AGGCTGCAGA AGCAAATAAG
    AATGCAAGTA TTCTTTTAAA GGAACTTGAT CTGGAAAAGT CAAGAGAGGA GAGCAGAAAG
    CAAGCTCTTG CAGCTAAAAG AGAGAAAAGA AAAGAAAAGA GAAAGAAGAA AAAAGAGGAG
    CAGAAAAGAA AGCAGGAAGA AGACGAGGAA AACAAACCTA AGGAGAATTC AGAACTACCG
    GAAGATGAAG ATGAAGAGGA GAATGAGGAA GATGTGGAGC AAGAAGTTCC CATAGAACCT
    CCTAGTGCGA CTACCACCAC CACAATTGGA ATCTCTGCAA CATCTGCAAC ATTCACAAAT
    GTGTTTGGGA AAAAAAGGGC CAATGTTGTG ACAACTCCTA GCACCAACCG CAAAAATAAG
    AAGAACAAAA CCAAAGAGAC TCCTCCCACT GCACATTTAA TATTACCAGA ACAACATATA
    TCCTTAGCAC AGCAAAAGGC AGATAAGAAT AAAATAAACG GAGAAAGCAA AGGTGGTGGT
    GCAGGTGGGA ATAGTGATTC AGATAACTTG GACAGCACAG ATTGCAACAG TGAGAGTAGC
    AGTGGTGGTA AAAGCCAAGA ATTAAATTTC ACTATGGATG TGAATTCTTC TAGCGATAGG
    AGATACCCCC TACTGCTACT CCATTCCCAG GAGGAAAAGA CAAGTACCGC TGCTTCCAAA
    ACTCCAACAC GACTTGAAGG CGAAGTGAAT CCTAATTCCT TATCAACAAC CTACAAGTCA
    GTGTCATTGC CATTAAGTTC TCCAAACATA AAGCTGAATC TGACTAGCCC TAAAAGGGGG
    CAAAAAAGAG AGGAAGGATG GAAAGAAGTT GTCCGAAGGT CAAAAAAATT ATCTGTTCCA
    GCCTCAGTGG TTTCCAGAAT AATGGGAAGA GGAGGATGCA ACATCACTGC AATTCAAGAT
    GTTACTGGTG CCCACATTGA TGTGGATAAA CAAAAAGATA AGAATGGCGA GAGAATGATC
    ACAATTAGGG GTGGTACAGA ATCAACAAGA TCTGCAGTTC AACTTATTAA TGCACTTATT
    CAAGATCCTG CTAAGGAACT GGAAGACTTG ATTCCTAAAA ACCATATCAG AACACCTGCC
    AGCATCAAAG CCATTCATGC AAACTTCTCA TCTGGAGTAG GCACCACAGC AGCTTCCAGT
    AAAAATGCAT TTCCTTTGGG TGCTCCAAGT CTGGTAACTT CACAGGCAAC AACATTATCT
    ACGTTCCAGC CCACTAATAA ACTTAATAAG AACGTTCCAG CAAACGTACG TTCTTCTTTC
    CCAGTTTCTC TGCCCTTAGC TTATCCCCAC CCGCATTTTG CCCTGTTGGC TGCTCAAACC
    ATGCAACAGA TTCGGCATCC TCGCTTACCC ATGGCCCAGT TTGGAGGAAC CTTCTCACCC
    TCTCCTAACA CTTGGGGACC ATTCCCAGTG AGACCTGTGA ATCCTGGCAA CACAAACAGC
    TCTCCAAAGC ATAATAATAC AGGCCGTCTA CCTAACCAGA ACGGAACTGT TCTACCGTCA
    GAGTCTACTG GACTAGCTAC TGCCAGTTGC CCTATCACTG TCTCTTCTGT AGTTGCTGCC
    AGTCAGCAAC TGTGTGTGAC TAATACCCGG ACTCCTTCAT CAGTCAGAAA GCAGCTGTTT
    GCCTGTGTGC CTAAGACAAG CCCTCCAGCA ACAGTGATTA CTTCTATGAC AAGCACTTGT
    AGTTCCCTGC CTTCAGTCTC CTCTGCACCT ATCACTAGTG GGCAAGTTCC CACCACATTT
    CTGCCTGCAA GTACTTCTCA AGCACAGCTT TCTTCACAAA AGATGGAGTC TTTCTCTGCC
    ATGCCCGCCC CCAAAGAGAA ACTGTCCACA CAGGACCAGT CCACGGCAAA CCTGTGTCCC
    CCCTCTTCAA CTGCCAACAG CTGCAGTAGC TCTGCCAGCA ACACCCCAGG AGCTCCAGAA
    ACTCACCCAT CTGGTAGTCC CACTCCTCTG TCCAATAACA CACAGGAGGA AGCACAGCCA
    TCCAGTGTGT CTGATTTAAG TCCTATGTCA ATGCCTTTTG CATCTAATTC AGAACCTGCT
    CCATTGACTT TAGCATCACC TAGATTGGTT GCTGCTGATA ATCAGGATAC CAGTAATTTA
    CCTCAGTTAG CTGTACCAGC ACCTCGAGTT TCTCATCGAT TGCAACCCAG AGGTTCTTTC
    TACTCAGTGG TACCAAATGC AACTATACAC CAGGATCCCC AGTCCATTTT TGTTACAAAT
    CCAGTTCCTT TAACACCACC TCAAGGCCCA CCAGCTACAG TGCAGCTTTC TTCAGCCATG
    AACATTATGA ATGGTTCTCA GATGCACATT AACCCAGCAA ATAAATCTTT GCCACCTACG
    TTTGGCCCGG CCACGCTTTT CAATCACTTC AGTAGTCTCT TTGATAGCAG TCAGGTGCCA
    GCTAACCAGG GTTGGGGAGA TGGTCCTCTG TCCTCACGAG TTGCTGCAGA TGCCTCTTTC
    ACTGTTCAGT CAGCATTTCT GGGTAGCTCT GTACTTGGAC ACTTGGAGAA CGTGCACCCT
    GACAATTCCA AGGCACCTGG CTTCAGATCA CCTTCCCAGC GAGTTTCTAC TAGTCCAGTT
    GGATTACCGT CCATTGACCC ATCAGGCAGC TCCCCATCTT CCTCTTCTGC TCCTCTGACA
    AGTTTTTCCA GCATACCAGG AACTCGGGTT TTCCTGCAAG GGCCAGCTCC TGTTGGGACC
    CCTAGTTTCA ACAGACAACA TTTTTCTCCC CACCCTTGGA CGAGCGCCTC AAACTCATCC
    ACTTCTGCCC CACCAACATT GGGCCAACCA AAAGGAGGCA GTGCCAGTCA GGATCGAAAA
    ATCCCTCCCC CCATTGGAAC AGAGAGACTA GCCCGAATTC GACAAGGAGG GTCTGTCGCA
    CAAGCCCCTG TGGGAACCGG TTTTGTCGCT CCTGTTGGAC ACAGTGGAAT CTGGTCATTT
    GGTGTCAATG CCATGTCAGA AGGGTTATCA GGTTGGTCAC AGTCTGTGAT AGGGAACCAT
    CCAATGCATC AACAATTGTC AGACCCAAGC ACATTCTCTC AGCATCAGCC AATGGAGAGA
    GATGATTCTG GAATGGTAGC CCCCTCTAAC ATTTTTCATC AGCCTATGGC AAGCAGTTTT
    GTGGATTTTT CTAAAGGTCT ACCAATTTCC ATGTATGGAG GCACCATAAT ACCCTCCCAT
    CCTCAGCTTG CTGATGTTCC AGGAGGCCCT CTGTTTAATG GACTTCACAA CCCAGATCCT
    TCTTGGAACC CTATGATAAA AGTTATTCAA AATTCAACTG AATGCACTGA TGCCCAGCAG
    ATTTGGCCTG GCACGTGGGC ACCTCATATT GGAAACATGC ATCTCAAATA TGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850985.1
    CDS148..7827
    Translation

    Target ORF information:

    RefSeq Version XM_024995217.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAC AAGCAGGAGG ACATGAAGAC TATTTTGGAG
    GGCATAGATC CGGCCAAGCA TCAGGAGCAT GAATCTGAAG GTGGAAGAAC ACCTTTAATG
    AAAGCTGCAA GAGCTGGTCA TTTGTGTACT GTGCAGTTTC TTATTAGCAA AGGTGCTAAT
    GTGAATAGGG CTACAGCCAA TAATGATCAT ACAGTAGTGT CACTGGCATG TGCAGGAGGT
    CACCTGGCAG TGGTGGAGCT ACTCTTGGCT CATGGGGCTG ACCCTACTCA TCGTCTCAAG
    GATGGCTCAA CAATGCTCAT TGAAGCTGCA AAAGGTGGTC ATACTAATGT TGTTTCTTAT
    CTTTTGGATT ATCCAAACAA TGTTCTGTCA GTTCCTACCA CAGATGTGTC TCAGCTTACC
    CCACCTTCTC AAGATCAATC TCAGGTTCCA CGTGTGCCAA TGCATACACT TGCCATGGTT
    GTACCTCCTC AGGAACCTGA CAGAACTTTA CAGGAGAACT CTCCTGCTGT TTTAGGAGTA
    CAAAAAGGTA CATCCAAGCA GAAGTCCAGT TCCCTGCAGG TAGCAGATCA GGACGTACTG
    CCACCTTTTC ACCCATACCA GCCTTTGGAG TGCATAGTAG AGGAGACTGA AGGAAAACTG
    AATGAACTGG GACAAAGAAT TAGTGCTATT GAAAAAGCAC AGCTTAAATC ATTGGAGTTA
    ATTCAAGGTG AACCTCTGAA CAAAGATAAG ATAGAAGAAC TTAAAAAGAA CAGAGAAGAG
    CAAGTCCAGA AGAAGAAGAA AATACTGAAA GAACTGCAGA AAGTGGAAAG GCAGTTGCAG
    ATGAAAACAC AGCAGCAATT TACCAAAGAA TATCTGGAAA CCAAAGGTCA GAAAGACACA
    GTATCTCTAC ACCAGCAGTG CTCTCATAGA GGAGTCTTCC CAGAAGGGGA AGGAGATGGT
    AGTCTCCCAG AGGATCACTT TTCAGAGTTA CCTCAGGTTG ACACAATCTT ATTTAAAGAT
    AATGATGTTG ATGATGAGCA ACAGTCTCCA CCATCGGCAG AACAAATTGA TTTTGTCCCA
    GTCCAGCCTT TATCATCTCC ACAGTGTAAC TTTTCCAGTG AATTAGGTTC TAATGGGACA
    AATTCTCTTG AACTTCAGAA AGTATCAGGT AATCATCAGA TTATAGGACA GCCTCAGATT
    GCTATTACTG GACATGATCA GGGGCTGTTA GTGCAAGAAC CAGATGGACT AATGGTTGCA
    ACTCCAGCCC AGACGCTTAC CGACACTCTT GATGACCTGA TAGCAGCTGT GAGTAGCAGA
    GTGCCCACTG GCTCAAACAG CTCTTCTCAA AACGTAGAAT GTCTTACACC TGAACCCTGT
    TCTCAGTCTC CAAGCAATGT GGCTTCTCAG TCTATACCCC CTGTGTATCC TTCAGTTGAC
    ATTGATGCAC ATACTGAGAG CAATCATGAC ACTGCATTAA CCCTAGCTTG TGCAGGTGGC
    CATGAAGAAC TTGTATCTGT ACTCATAGCA CGAGATGCTA AAATTGAACA CAGAGACAAA
    AAAGGTTTCA CACCACTGAT CCTGGCAGCA ACAGCAGGAC ATGTTGGAGT TGTTGAAATC
    CTTTTGGATA AAGGTGGAGA CATAGAAGCA CAATCTGAAC GAACTAAGGA TACTCCTCTT
    TCATTAGCAT GTTCTGGTGG ACGTCAGGAA GTGGTAGACT TGCTGTTGGC TCGGGGTGCA
    AATAAAGAAC ATAGGAACGT GTCTGATTAT ACACCGCTGA GTCTAGCTGC ATCTGGAGGA
    TATGTTAATA TCATTAAGAT TCTGCTTAAT GCTGGGGCAG AAATTAATTC AAGGACTGGG
    AGTAAACTAG GTATTTCTCC CCTGATGTTG GCTGCAATGA ATGGACATGT TCCTGCAGTG
    AAACTGCTGC TTGATATGGG TTCAGATATT AATGCCCAAA TAGAGACCAA TCGGAACACA
    GCTCTCACCT TGGCCTGTTT CCAAGGCCGA GCGGAAGTAG TGAGCTTGCT TCTGGACCGA
    AAAGCCAATG TTGAACATAG GGCAAAGACT GGTCTTACCC CCTTGATGGA AGCTGCTTCT
    GGGGGATATG CAGAGGTTGG AAGAGTTCTC CTAGATAAAG GAGCAGATGT CAATGCCCCC
    CCTGTGCCTT CCTCAAGAGA CACTGCTTTA ACAATAGCAG CGGACAAAGG TCACTACAAA
    TTTTGTGAGC TCTTGATTAA TAGGGGAGCC CATATTGATG TTCGTAACAA GAAGGGAAAC
    ACACCACTTT GGTTGGCATC CAATGGTGGT CATTTTGAAG TGGTGCAGTT GCTAGTGCGA
    TCAGGTGCTG ATGTGGATGG AGCTGATAAC CGGAAAATCA CACCTCTTAT GTCAGCATTT
    CGAAAGGGTC ATGTAAAAGT TGTTCAGTAT TTGGTTAAGG AAGTCAATCA GTTCCCTTCT
    GATATAGAAT GCATGAGATA CATAGCAACA ATCACTGATA AGGAACTGTT GAAAAAATGT
    CACCAATGTG TTGAGACAAT TGTGAAGGCT AAAGACCAAC AGGCTGCAGA AGCAAATAAG
    AATGCAAGTA TTCTTTTAAA GGAACTTGAT CTGGAAAAGT CAAGAGAGGA GAGCAGAAAG
    CAAGCTCTTG CAGCTAAAAG AGAGAAAAGA AAAGAAAAGA GAAAGAAGAA AAAAGAGGAG
    CAGAAAAGAA AGCAGGAAGA AGACGAGGAA AACAAACCTA AGGAGAATTC AGAACTACCG
    GAAGATGAAG ATGAAGAGGA GAATGAGGAA GATGTGGAGC AAGAAGTTCC CATAGAACCT
    CCTAGTGCGA CTACCACCAC CACAATTGGA ATCTCTGCAA CATCTGCAAC ATTCACAAAT
    GTGTTTGGGA AAAAAAGGGC CAATGTTGTG ACAACTCCTA GCACCAACCG CAAAAATAAG
    AAGAACAAAA CCAAAGAGAC TCCTCCCACT GCACATTTAA TATTACCAGA ACAACATATA
    TCCTTAGCAC AGCAAAAGGC AGATAAGAAT AAAATAAACG GAGAAAGCAA AGGTGGTGGT
    GCAGGTGGGA ATAGTGATTC AGATAACTTG GACAGCACAG ATTGCAACAG TGAGAGTAGC
    AGTGGTGGTA AAAGCCAAGA ATTAAATTTC ACTATGGATG TGAATTCTTC TAGCGATAGG
    AGATACCCCC TACTGCTACT CCATTCCCAG GAGGAAAAGA CAAGTACCGC TGCTTCCAAA
    ACTCCAACAC GACTTGAAGG CGAAGTGAAT CCTAATTCCT TATCAACAAC CTACAAGTCA
    GTGTCATTGC CATTAAGTTC TCCAAACATA AAGCTGAATC TGACTAGCCC TAAAAGGGGG
    CAAAAAAGAG AGGAAGGATG GAAAGAAGTT GTCCGAAGGT CAAAAAAATT ATCTGTTCCA
    GCCTCAGTGG TTTCCAGAAT AATGGGAAGA GGAGGATGCA ACATCACTGC AATTCAAGAT
    GTTACTGGTG CCCACATTGA TGTGGATAAA CAAAAAGATA AGAATGGCGA GAGAATGATC
    ACAATTAGGG GTGGTACAGA ATCAACAAGA TCTGCAGTTC AACTTATTAA TGCACTTATT
    CAAGATCCTG CTAAGGAACT GGAAGACTTG ATTCCTAAAA ACCATATCAG AACACCTGCC
    AGCATCAAAG CCATTCATGC AAACTTCTCA TCTGGAGTAG GCACCACAGC AGCTTCCAGT
    AAAAATGCAT TTCCTTTGGG TGCTCCAAGT CTGGTAACTT CACAGGCAAC AACATTATCT
    ACGTTCCAGC CCACTAATAA ACTTAATAAG AACGTTCCAG CAAACGTACG TTCTTCTTTC
    CCAGTTTCTC TGCCCTTAGC TTATCCCCAC CCGCATTTTG CCCTGTTGGC TGCTCAAACC
    ATGCAACAGA TTCGGCATCC TCGCTTACCC ATGGCCCAGT TTGGAGGAAC CTTCTCACCC
    TCTCCTAACA CTTGGGGACC ATTCCCAGTG AGACCTGTGA ATCCTGGCAA CACAAACAGC
    TCTCCAAAGC ATAATAATAC AGGCCGTCTA CCTAACCAGA ACGGAACTGT TCTACCGTCA
    GAGTCTACTG GACTAGCTAC TGCCAGTTGC CCTATCACTG TCTCTTCTGT AGTTGCTGCC
    AGTCAGCAAC TGTGTGTGAC TAATACCCGG ACTCCTTCAT CAGTCAGAAA GCAGCTGTTT
    GCCTGTGTGC CTAAGACAAG CCCTCCAGCA ACAGTGATTA CTTCTATGAC AAGCACTTGT
    AGTTCCCTGC CTTCAGTCTC CTCTGCACCT ATCACTAGTG GGCAAGTTCC CACCACATTT
    CTGCCTGCAA GTACTTCTCA AGCACAGCTT TCTTCACAAA AGATGGAGTC TTTCTCTGCC
    ATGCCCGCCC CCAAAGAGAA ACTGTCCACA CAGGACCAGT CCACGGCAAA CCTGTGTCCC
    CCCTCTTCAA CTGCCAACAG CTGCAGTAGC TCTGCCAGCA ACACCCCAGG AGCTCCAGAA
    ACTCACCCAT CTGGTAGTCC CACTCCTCTG TCCAATAACA CACAGGAGGA AGCACAGCCA
    TCCAGTGTGT CTGATTTAAG TCCTATGTCA ATGCCTTTTG CATCTAATTC AGAACCTGCT
    CCATTGACTT TAGCATCACC TAGATTGGTT GCTGCTGATA ATCAGGATAC CAGTAATTTA
    CCTCAGTTAG CTGTACCAGC ACCTCGAGTT TCTCATCGAT TGCAACCCAG AGGTTCTTTC
    TACTCAGTGG TACCAAATGC AACTATACAC CAGGATCCCC AGTCCATTTT TGTTACAAAT
    CCAGTTCCTT TAACACCACC TCAAGGCCCA CCAGCTACAG TGCAGCTTTC TTCAGCCATG
    AACATTATGA ATGGTTCTCA GATGCACATT AACCCAGCAA ATAAATCTTT GCCACCTACG
    TTTGGCCCGG CCACGCTTTT CAATCACTTC AGTAGTCTCT TTGATAGCAG TCAGGTGCCA
    GCTAACCAGG GTTGGGGAGA TGGTCCTCTG TCCTCACGAG TTGCTGCAGA TGCCTCTTTC
    ACTGTTCAGT CAGCATTTCT GGGTAGCTCT GTACTTGGAC ACTTGGAGAA CGTGCACCCT
    GACAATTCCA AGGCACCTGG CTTCAGATCA CCTTCCCAGC GAGTTTCTAC TAGTCCAGTT
    GGATTACCGT CCATTGACCC ATCAGGCAGC TCCCCATCTT CCTCTTCTGC TCCTCTGACA
    AGTTTTTCCA GCATACCAGG AACTCGGGTT TTCCTGCAAG GGCCAGCTCC TGTTGGGACC
    CCTAGTTTCA ACAGACAACA TTTTTCTCCC CACCCTTGGA CGAGCGCCTC AAACTCATCC
    ACTTCTGCCC CACCAACATT GGGCCAACCA AAAGGAGGCA GTGCCAGTCA GGATCGAAAA
    ATCCCTCCCC CCATTGGAAC AGAGAGACTA GCCCGAATTC GACAAGGAGG GTCTGTCGCA
    CAAGCCCCTG TGGGAACCGG TTTTGTCGCT CCTGTTGGAC ACAGTGGAAT CTGGTCATTT
    GGTGTCAATG CCATGTCAGA AGGGTTATCA GGTTGGTCAC AGTCTGTGAT AGGGAACCAT
    CCAATGCATC AACAATTGTC AGACCCAAGC ACATTCTCTC AGCATCAGCC AATGGAGAGA
    GATGATTCTG GAATGGTAGC CCCCTCTAAC ATTTTTCATC AGCCTATGGC AAGCAGTTTT
    GTGGATTTTT CTAAAGGTCT ACCAATTTCC ATGTATGGAG GCACCATAAT ACCCTCCCAT
    CCTCAGCTTG CTGATGTTCC AGGAGGCCCT CTGTTTAATG GACTTCACAA CCCAGATCCT
    TCTTGGAACC CTATGATAAA AGTTATTCAA AATTCAACTG AATGCACTGA TGCCCAGCAG
    ATTTGGCCTG GCACGTGGGC ACCTCATATT GGAAACATGC ATCTCAAATA TGTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236727
    Clone ID Related Accession (Same CDS sequence) XM_005209497.4
    Accession Version XM_005209497.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and KH domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TCCACTTCTG CCCCACCAAC ATTGGGCCAA CCAAAAGGAG GCAGTGCCAG TCAGGATCGA
    AAAATCCCTC CCCCCATTGG AACAGAGAGA CTAGCCCGAA TTCGACAAGG AGGGTCTGTC
    GCACAAGCCC CTGTGGGAAC CGGTTTTGTC GCTCCTGTTG GACACAGTGG AATCTGGTCA
    TTTGGTGTCA ATGCCATGTC AGAAGGGTTA TCAGGTTGGT CACAGTCTGT GATAGGGAAC
    CATCCAATGC ATCAACAATT GTCAGACCCA AGCACATTCT CTCAGCATCA GCCAATGGAG
    AGAGATGATT CTGGAATGGT AGCCCCCTCT AACATTTTTC ATCAGCCTAT GGCAAGCAGT
    TTTGTGGATT TTTCTAAAGG TCTACCAATT TCCATGTATG GAGGCACCAT AATACCCTCC
    CATCCTCAGC TTGCTGATGT TCCAGGAGGC CCTCTGTTTA ATGGACTTCA CAACCCAGAT
    CCTTCTTGGA ACCCTATGAT AAAAGTTATT CAAAATTCAA CTGAATGCAC TGATGCCCAG
    CAGATTTGGC CTGGCACGTG GGCACCTCAT ATTGGAAACA TGCATCTCAA ATATGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209554.1
    CDS158..7780
    Translation

    Target ORF information:

    RefSeq Version XM_005209497.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209497.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TCCACTTCTG CCCCACCAAC ATTGGGCCAA CCAAAAGGAG GCAGTGCCAG TCAGGATCGA
    AAAATCCCTC CCCCCATTGG AACAGAGAGA CTAGCCCGAA TTCGACAAGG AGGGTCTGTC
    GCACAAGCCC CTGTGGGAAC CGGTTTTGTC GCTCCTGTTG GACACAGTGG AATCTGGTCA
    TTTGGTGTCA ATGCCATGTC AGAAGGGTTA TCAGGTTGGT CACAGTCTGT GATAGGGAAC
    CATCCAATGC ATCAACAATT GTCAGACCCA AGCACATTCT CTCAGCATCA GCCAATGGAG
    AGAGATGATT CTGGAATGGT AGCCCCCTCT AACATTTTTC ATCAGCCTAT GGCAAGCAGT
    TTTGTGGATT TTTCTAAAGG TCTACCAATT TCCATGTATG GAGGCACCAT AATACCCTCC
    CATCCTCAGC TTGCTGATGT TCCAGGAGGC CCTCTGTTTA ATGGACTTCA CAACCCAGAT
    CCTTCTTGGA ACCCTATGAT AAAAGTTATT CAAAATTCAA CTGAATGCAC TGATGCCCAG
    CAGATTTGGC CTGGCACGTG GGCACCTCAT ATTGGAAACA TGCATCTCAA ATATGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236728
    Clone ID Related Accession (Same CDS sequence) XM_024995218.1
    Accession Version XM_024995218.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and KH domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TGTGACTCTC CTATTCCATC TGTTTCTTCG GGATCATCTT CACCTCTTTC AGCCACTTCT
    GCCCCACCAA CATTGGGCCA ACCAAAAGGA GGCAGTGCCA GTCAGGATCG AAAAATCCCT
    CCCCCCATTG GAACAGAGAG ACTAGCCCGA ATTCGACAAG GAGGGTCTGT CGCACAAGCC
    CCTGTGGGAA CCGGTTTTGT CGCTCCTGTT GGACACAGTG GAATCTGGTC ATTTGGTGTC
    AATGCCATGT CAGAAGGGTT ATCAGGTTGG TCACAGTCTG TGATAGGGAA CCATCCAATG
    CATCAACAAT TGTCAGACCC AAGCACATTC TCTCAGCATC AGCCAATGGA GAGAGATGAT
    TCTGGAATGG TAGCCCCCTC TAACATTTTT CATCAGCCTA TGGCAAGCAG TTTTGTGGAT
    TTTTCTAAAG GTCTACCAAT TTCCATGTAT GGAGGCACCA TAATACCCTC CCATCCTCAG
    CTTGCTGATG TTCCAGGAGG CCCTCTGTTT AATGGACTTC ACAACCCAGA TCCTTCTTGG
    AACCCTATGA TAAAAGTTAT TCAAAATTCA ACTGAATGCA CTGATGCCCA GCAGATTTGG
    CCTGGCACGT GGGCACCTCA TATTGGAAAC ATGCATCTCA AATATGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850986.1
    CDS158..7831
    Translation

    Target ORF information:

    RefSeq Version XM_024995218.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCCGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TGTGACTCTC CTATTCCATC TGTTTCTTCG GGATCATCTT CACCTCTTTC AGCCACTTCT
    GCCCCACCAA CATTGGGCCA ACCAAAAGGA GGCAGTGCCA GTCAGGATCG AAAAATCCCT
    CCCCCCATTG GAACAGAGAG ACTAGCCCGA ATTCGACAAG GAGGGTCTGT CGCACAAGCC
    CCTGTGGGAA CCGGTTTTGT CGCTCCTGTT GGACACAGTG GAATCTGGTC ATTTGGTGTC
    AATGCCATGT CAGAAGGGTT ATCAGGTTGG TCACAGTCTG TGATAGGGAA CCATCCAATG
    CATCAACAAT TGTCAGACCC AAGCACATTC TCTCAGCATC AGCCAATGGA GAGAGATGAT
    TCTGGAATGG TAGCCCCCTC TAACATTTTT CATCAGCCTA TGGCAAGCAG TTTTGTGGAT
    TTTTCTAAAG GTCTACCAAT TTCCATGTAT GGAGGCACCA TAATACCCTC CCATCCTCAG
    CTTGCTGATG TTCCAGGAGG CCCTCTGTTT AATGGACTTC ACAACCCAGA TCCTTCTTGG
    AACCCTATGA TAAAAGTTAT TCAAAATTCA ACTGAATGCA CTGATGCCCA GCAGATTTGG
    CCTGGCACGT GGGCACCTCA TATTGGAAAC ATGCATCTCA AATATGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21575
    Clone ID Related Accession (Same CDS sequence) XM_005209497.3 , XM_015464301.1
    Accession Version XM_005209497.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: ankyrin repeat and KH domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104035.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCAGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TCCACTTCTG CCCCACCAAC ATTGGGCCAA CCAAAAGGAG GCAGTGCCAG TCAGGATCGA
    AAAATCCCTC CCCCCATTGG AACAGAGAGA CTAGCCCGAA TTCGACAAGG AGGGTCTGTC
    GCACAAGCCC CTGTGGGAAC CGGTTTTGTC GCTCCTGTTG GACACAGTGG AATCTGGTCA
    TTTGGTGTCA ATGCCATGTC AGAAGGGTTA TCAGGTTGGT CACAGTCTGT GATAGGGAAC
    CATCCAATGC ATCAACAATT GTCAGACCCA AGCACATTCT CTCAGCATCA GCCAATGGAG
    AGAGATGATT CTGGAATGGT AGCCCCCTCT AACATTTTTC ATCAGCCTAT GGCAAGCAGT
    TTTGTGGATT TTTCTAAAGG TCTACCAATT TCCATGTATG GAGGCACCAT AATACCCTCC
    CATCCTCAGC TTGCTGATGT TCCAGGAGGC CCTCTGTTTA ATGGACTTCA CAACCCAGAT
    CCTTCTTGGA ACCCTATGAT AAAAGTTATT CAAAATTCAA CTGAATGCAC TGATGCCCAG
    CAGATTTGGC CTGGCACGTG GGCACCTCAT ATTGGAAACA TGCATCTCAA ATATGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209554.1
    CDS1..7623
    Translation

    Target ORF information:

    RefSeq Version XM_005209497.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCAGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TCCACTTCTG CCCCACCAAC ATTGGGCCAA CCAAAAGGAG GCAGTGCCAG TCAGGATCGA
    AAAATCCCTC CCCCCATTGG AACAGAGAGA CTAGCCCGAA TTCGACAAGG AGGGTCTGTC
    GCACAAGCCC CTGTGGGAAC CGGTTTTGTC GCTCCTGTTG GACACAGTGG AATCTGGTCA
    TTTGGTGTCA ATGCCATGTC AGAAGGGTTA TCAGGTTGGT CACAGTCTGT GATAGGGAAC
    CATCCAATGC ATCAACAATT GTCAGACCCA AGCACATTCT CTCAGCATCA GCCAATGGAG
    AGAGATGATT CTGGAATGGT AGCCCCCTCT AACATTTTTC ATCAGCCTAT GGCAAGCAGT
    TTTGTGGATT TTTCTAAAGG TCTACCAATT TCCATGTATG GAGGCACCAT AATACCCTCC
    CATCCTCAGC TTGCTGATGT TCCAGGAGGC CCTCTGTTTA ATGGACTTCA CAACCCAGAT
    CCTTCTTGGA ACCCTATGAT AAAAGTTATT CAAAATTCAA CTGAATGCAC TGATGCCCAG
    CAGATTTGGC CTGGCACGTG GGCACCTCAT ATTGGAAACA TGCATCTCAA ATATGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21575
    Clone ID Related Accession (Same CDS sequence) XM_005209497.3 , XM_015464301.1
    Accession Version XM_015464301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: ankyrin repeat and KH domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCAGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TCCACTTCTG CCCCACCAAC ATTGGGCCAA CCAAAAGGAG GCAGTGCCAG TCAGGATCGA
    AAAATCCCTC CCCCCATTGG AACAGAGAGA CTAGCCCGAA TTCGACAAGG AGGGTCTGTC
    GCACAAGCCC CTGTGGGAAC CGGTTTTGTC GCTCCTGTTG GACACAGTGG AATCTGGTCA
    TTTGGTGTCA ATGCCATGTC AGAAGGGTTA TCAGGTTGGT CACAGTCTGT GATAGGGAAC
    CATCCAATGC ATCAACAATT GTCAGACCCA AGCACATTCT CTCAGCATCA GCCAATGGAG
    AGAGATGATT CTGGAATGGT AGCCCCCTCT AACATTTTTC ATCAGCCTAT GGCAAGCAGT
    TTTGTGGATT TTTCTAAAGG TCTACCAATT TCCATGTATG GAGGCACCAT AATACCCTCC
    CATCCTCAGC TTGCTGATGT TCCAGGAGGC CCTCTGTTTA ATGGACTTCA CAACCCAGAT
    CCTTCTTGGA ACCCTATGAT AAAAGTTATT CAAAATTCAA CTGAATGCAC TGATGCCCAG
    CAGATTTGGC CTGGCACGTG GGCACCTCAT ATTGGAAACA TGCATCTCAA ATATGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319787.1
    CDS1..7623
    Translation

    Target ORF information:

    RefSeq Version XM_015464301.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and KH domain containing 1 (ANKHD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGCTGACCG ACGGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACTTGGA CTCGGTGGCT 
    CCGCGATCCG CCCCAGCAGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGGCTGGGA
    ATCCGCACTG TGAGACTCTT CGGGGAGGCC GGGCCGGCTC CGGGAGTCGG TGGAGGCAGC
    AGCGGTGCAG GCGGAGGGGA CGCGGCGCTG GATTTCAAGT TGGCGGCTGC TGTCCTGAGG
    ACCGGGGGTG GAGGTGGTGC CTCTGGTAGC GACGAGGACG AGGTGTCCGA GGTTGAATCA
    TTTATTTTGG ACCAAGAAGA TTTGGATAAC CCAGTGCTTA AAACAACATC AGAGATATTC
    TTATCAAGCA CTGCAGAAGG AGCAGACTTA CGCACTGTGG ATCCAGAGAC ACAGGCACGA
    CTAGAAGCCT TGCTGGAAGC AGCAGGAATT GGCAAATTGT CAACTGCTGA TGGTAAAGCC
    TTTGCAGATC CTGAGGTACT CCGAAGACTG ACATCCTCAG TAAGTTGTGC ACTGGATGAA
    GCTGCCGCTG CACTGACGCG GATGAGGGCG GAAAACAGTC ACAGTGCAGG ACAAGTAGAC
    ACTCGCAGTC TGGCAGAAGC TTGTTCTGAT GGGGATGTGA ATGCTGTTCG TAAACTGCTA
    GATGAAGGCA GAAGTGTAAA TGAACATACT GAAGAAGGAG AAAGCCTGCT GTGTTTGGCT
    TGTTCAGCTG GGTATTATGA ATTAGCACAA GTATTGCTTG CTATGCATGC TAATGTTGAA
    GACAGAGGGA ATAAAGGAGA TATAACTCCC CTAATGGCAG CATCTAGTGG AGGTTATTTA
    GATATTGTGA AATTGTTGCT TCTTCATGAT GCAGATGTCA ACTCACAGTC TGCAACAGGC
    AACACTGCAT TAACTTATGC CTGTGCTGGA GGATTTGTTG ATATTGTGAA AGTGCTACTT
    AATGAAGGCG CAAATATAGA AGATCATAAT GAAAATGGAC ATACCCCCTT AATGGAAGCA
    GCCAGTGCAG GTCATGTGGA AGTTGCAAGA GTTCTTCTAG ATCATGGGGC AGGCATCAAC
    ACTCATTCTA ATGAATTCAA AGAAAGTGCT CTTACACTTG CATGCTACAA AGGCCATTTG
    GACATGGTTC GCTTTCTGCT TGATGCTGGT GCAGATCAAG AGCACAAAAC AGATGAGATG
    CACACTGCCT TAATGGAGGC TTGCATGGAT GGACATGTAG AGGTAGCACG TTTGCTTTTG
    GATAGTGGTG CTCAAGTGAA TATGCCTGCG GATTCATTCG AGTCGCCGTT AACTCTGGCT
    GCCTGTGGAG GACATGTTGA ATTGGCAGCT CTGCTTATTG AAAGGGGAGC AAATCTTGAA
    GAAGTTAATG ATGAAGGATA TACTCCTTTG ATGGAAGCTG CTCGGGAGGG ACATGAAGAG
    ATGGTGGCAC TACTCTTAGC GCAAGGAGCA AATATAAATG CTCAGACAGA AGAAACCCAA
    GAAACTGCTC TGACGTTGGC TTGCTGTGGA GGTTTTTCTG AAGTTGCAGA CTTCCTGATT
    AAGGCGGGGG CTGACATAGA ACTCGGCTGT TCCACACCTC TGATGGAGGC TTCTCAGGAG
    GGACACCTGG AGTTGGTTAA ATACTTACTG GCTGCTGGTG CTAATGTTCA TGCTACAACA
    GCAACAGGAG ACACAGCTTT AACCTACGCT TGTGAAAATG GACACACGGA TGTTGCAGAT
    GTTTTACTTC AAGCAGGGGC CGATTTAGAG CATGAATCTG AAGGTGGAAG AACACCTTTA
    ATGAAAGCTG CAAGAGCTGG TCATTTGTGT ACTGTGCAGT TTCTTATTAG CAAAGGTGCT
    AATGTGAATA GGGCTACAGC CAATAATGAT CATACAGTAG TGTCACTGGC ATGTGCAGGA
    GGTCACCTGG CAGTGGTGGA GCTACTCTTG GCTCATGGGG CTGACCCTAC TCATCGTCTC
    AAGGATGGCT CAACAATGCT CATTGAAGCT GCAAAAGGTG GTCATACTAA TGTTGTTTCT
    TATCTTTTGG ATTATCCAAA CAATGTTCTG TCAGTTCCTA CCACAGATGT GTCTCAGCTT
    ACCCCACCTT CTCAAGATCA ATCTCAGGTT CCACGTGTGC CAATGCATAC ACTTGCCATG
    GTTGTACCTC CTCAGGAACC TGACAGAACT TTACAGGAGA ACTCTCCTGC TGTTTTAGGA
    GTACAAAAAG GTACATCCAA GCAGAAGTCC AGTTCCCTGC AGGTAGCAGA TCAGGACGTA
    CTGCCACCTT TTCACCCATA CCAGCCTTTG GAGTGCATAG TAGAGGAGAC TGAAGGAAAA
    CTGAATGAAC TGGGACAAAG AATTAGTGCT ATTGAAAAAG CACAGCTTAA ATCATTGGAG
    TTAATTCAAG GTGAACCTCT GAACAAAGAT AAGATAGAAG AACTTAAAAA GAACAGAGAA
    GAGCAAGTCC AGAAGAAGAA GAAAATACTG AAAGAACTGC AGAAAGTGGA AAGGCAGTTG
    CAGATGAAAA CACAGCAGCA ATTTACCAAA GAATATCTGG AAACCAAAGG TCAGAAAGAC
    ACAGTATCTC TACACCAGCA GTGCTCTCAT AGAGGAGTCT TCCCAGAAGG GGAAGGAGAT
    GGTAGTCTCC CAGAGGATCA CTTTTCAGAG TTACCTCAGG TTGACACAAT CTTATTTAAA
    GATAATGATG TTGATGATGA GCAACAGTCT CCACCATCGG CAGAACAAAT TGATTTTGTC
    CCAGTCCAGC CTTTATCATC TCCACAGTGT AACTTTTCCA GTGAATTAGG TTCTAATGGG
    ACAAATTCTC TTGAACTTCA GAAAGTATCA GGTAATCATC AGATTATAGG ACAGCCTCAG
    ATTGCTATTA CTGGACATGA TCAGGGGCTG TTAGTGCAAG AACCAGATGG ACTAATGGTT
    GCAACTCCAG CCCAGACGCT TACCGACACT CTTGATGACC TGATAGCAGC TGTGAGTAGC
    AGAGTGCCCA CTGGCTCAAA CAGCTCTTCT CAAAACGTAG AATGTCTTAC ACCTGAACCC
    TGTTCTCAGT CTCCAAGCAA TGTGGCTTCT CAGTCTATAC CCCCTGTGTA TCCTTCAGTT
    GACATTGATG CACATACTGA GAGCAATCAT GACACTGCAT TAACCCTAGC TTGTGCAGGT
    GGCCATGAAG AACTTGTATC TGTACTCATA GCACGAGATG CTAAAATTGA ACACAGAGAC
    AAAAAAGGTT TCACACCACT GATCCTGGCA GCAACAGCAG GACATGTTGG AGTTGTTGAA
    ATCCTTTTGG ATAAAGGTGG AGACATAGAA GCACAATCTG AACGAACTAA GGATACTCCT
    CTTTCATTAG CATGTTCTGG TGGACGTCAG GAAGTGGTAG ACTTGCTGTT GGCTCGGGGT
    GCAAATAAAG AACATAGGAA CGTGTCTGAT TATACACCGC TGAGTCTAGC TGCATCTGGA
    GGATATGTTA ATATCATTAA GATTCTGCTT AATGCTGGGG CAGAAATTAA TTCAAGGACT
    GGGAGTAAAC TAGGTATTTC TCCCCTGATG TTGGCTGCAA TGAATGGACA TGTTCCTGCA
    GTGAAACTGC TGCTTGATAT GGGTTCAGAT ATTAATGCCC AAATAGAGAC CAATCGGAAC
    ACAGCTCTCA CCTTGGCCTG TTTCCAAGGC CGAGCGGAAG TAGTGAGCTT GCTTCTGGAC
    CGAAAAGCCA ATGTTGAACA TAGGGCAAAG ACTGGTCTTA CCCCCTTGAT GGAAGCTGCT
    TCTGGGGGAT ATGCAGAGGT TGGAAGAGTT CTCCTAGATA AAGGAGCAGA TGTCAATGCC
    CCCCCTGTGC CTTCCTCAAG AGACACTGCT TTAACAATAG CAGCGGACAA AGGTCACTAC
    AAATTTTGTG AGCTCTTGAT TAATAGGGGA GCCCATATTG ATGTTCGTAA CAAGAAGGGA
    AACACACCAC TTTGGTTGGC ATCCAATGGT GGTCATTTTG AAGTGGTGCA GTTGCTAGTG
    CGATCAGGTG CTGATGTGGA TGGAGCTGAT AACCGGAAAA TCACACCTCT TATGTCAGCA
    TTTCGAAAGG GTCATGTAAA AGTTGTTCAG TATTTGGTTA AGGAAGTCAA TCAGTTCCCT
    TCTGATATAG AATGCATGAG ATACATAGCA ACAATCACTG ATAAGGAACT GTTGAAAAAA
    TGTCACCAAT GTGTTGAGAC AATTGTGAAG GCTAAAGACC AACAGGCTGC AGAAGCAAAT
    AAGAATGCAA GTATTCTTTT AAAGGAACTT GATCTGGAAA AGTCAAGAGA GGAGAGCAGA
    AAGCAAGCTC TTGCAGCTAA AAGAGAGAAA AGAAAAGAAA AGAGAAAGAA GAAAAAAGAG
    GAGCAGAAAA GAAAGCAGGA AGAAGACGAG GAAAACAAAC CTAAGGAGAA TTCAGAACTA
    CCGGAAGATG AAGATGAAGA GGAGAATGAG GAAGATGTGG AGCAAGAAGT TCCCATAGAA
    CCTCCTAGTG CGACTACCAC CACCACAATT GGAATCTCTG CAACATCTGC AACATTCACA
    AATGTGTTTG GGAAAAAAAG GGCCAATGTT GTGACAACTC CTAGCACCAA CCGCAAAAAT
    AAGAAGAACA AAACCAAAGA GACTCCTCCC ACTGCACATT TAATATTACC AGAACAACAT
    ATATCCTTAG CACAGCAAAA GGCAGATAAG AATAAAATAA ACGGAGAAAG CAAAGGTGGT
    GGTGCAGGTG GGAATAGTGA TTCAGATAAC TTGGACAGCA CAGATTGCAA CAGTGAGAGT
    AGCAGTGGTG GTAAAAGCCA AGAATTAAAT TTCACTATGG ATGTGAATTC TTCTAGCGAT
    AGGAGATACC CCCTACTGCT ACTCCATTCC CAGGAGGAAA AGACAAGTAC CGCTGCTTCC
    AAAACTCCAA CACGACTTGA AGGCGAAGTG AATCCTAATT CCTTATCAAC AACCTACAAG
    TCAGTGTCAT TGCCATTAAG TTCTCCAAAC ATAAAGCTGA ATCTGACTAG CCCTAAAAGG
    GGGCAAAAAA GAGAGGAAGG ATGGAAAGAA GTTGTCCGAA GGTCAAAAAA ATTATCTGTT
    CCAGCCTCAG TGGTTTCCAG AATAATGGGA AGAGGAGGAT GCAACATCAC TGCAATTCAA
    GATGTTACTG GTGCCCACAT TGATGTGGAT AAACAAAAAG ATAAGAATGG CGAGAGAATG
    ATCACAATTA GGGGTGGTAC AGAATCAACA AGATCTGCAG TTCAACTTAT TAATGCACTT
    ATTCAAGATC CTGCTAAGGA ACTGGAAGAC TTGATTCCTA AAAACCATAT CAGAACACCT
    GCCAGCATCA AAGCCATTCA TGCAAACTTC TCATCTGGAG TAGGCACCAC AGCAGCTTCC
    AGTAAAAATG CATTTCCTTT GGGTGCTCCA AGTCTGGTAA CTTCACAGGC AACAACATTA
    TCTACGTTCC AGCCCACTAA TAAACTTAAT AAGAACGTTC CAGCAAACGT ACGTTCTTCT
    TTCCCAGTTT CTCTGCCCTT AGCTTATCCC CACCCGCATT TTGCCCTGTT GGCTGCTCAA
    ACCATGCAAC AGATTCGGCA TCCTCGCTTA CCCATGGCCC AGTTTGGAGG AACCTTCTCA
    CCCTCTCCTA ACACTTGGGG ACCATTCCCA GTGAGACCTG TGAATCCTGG CAACACAAAC
    AGCTCTCCAA AGCATAATAA TACAGGCCGT CTACCTAACC AGAACGGAAC TGTTCTACCG
    TCAGAGTCTA CTGGACTAGC TACTGCCAGT TGCCCTATCA CTGTCTCTTC TGTAGTTGCT
    GCCAGTCAGC AACTGTGTGT GACTAATACC CGGACTCCTT CATCAGTCAG AAAGCAGCTG
    TTTGCCTGTG TGCCTAAGAC AAGCCCTCCA GCAACAGTGA TTACTTCTAT GACAAGCACT
    TGTAGTTCCC TGCCTTCAGT CTCCTCTGCA CCTATCACTA GTGGGCAAGT TCCCACCACA
    TTTCTGCCTG CAAGTACTTC TCAAGCACAG CTTTCTTCAC AAAAGATGGA GTCTTTCTCT
    GCCATGCCCG CCCCCAAAGA GAAACTGTCC ACACAGGACC AGTCCACGGC AAACCTGTGT
    CCCCCCTCTT CAACTGCCAA CAGCTGCAGT AGCTCTGCCA GCAACACCCC AGGAGCTCCA
    GAAACTCACC CATCTGGTAG TCCCACTCCT CTGTCCAATA ACACACAGGA GGAAGCACAG
    CCATCCAGTG TGTCTGATTT AAGTCCTATG TCAATGCCTT TTGCATCTAA TTCAGAACCT
    GCTCCATTGA CTTTAGCATC ACCTAGATTG GTTGCTGCTG ATAATCAGGA TACCAGTAAT
    TTACCTCAGT TAGCTGTACC AGCACCTCGA GTTTCTCATC GATTGCAACC CAGAGGTTCT
    TTCTACTCAG TGGTACCAAA TGCAACTATA CACCAGGATC CCCAGTCCAT TTTTGTTACA
    AATCCAGTTC CTTTAACACC ACCTCAAGGC CCACCAGCTA CAGTGCAGCT TTCTTCAGCC
    ATGAACATTA TGAATGGTTC TCAGATGCAC ATTAACCCAG CAAATAAATC TTTGCCACCT
    ACGTTTGGCC CGGCCACGCT TTTCAATCAC TTCAGTAGTC TCTTTGATAG CAGTCAGGTG
    CCAGCTAACC AGGGTTGGGG AGATGGTCCT CTGTCCTCAC GAGTTGCTGC AGATGCCTCT
    TTCACTGTTC AGTCAGCATT TCTGGGTAGC TCTGTACTTG GACACTTGGA GAACGTGCAC
    CCTGACAATT CCAAGGCACC TGGCTTCAGA TCACCTTCCC AGCGAGTTTC TACTAGTCCA
    GTTGGATTAC CGTCCATTGA CCCATCAGGC AGCTCCCCAT CTTCCTCTTC TGCTCCTCTG
    ACAAGTTTTT CCAGCATACC AGGAACTCGG GTTTTCCTGC AAGGGCCAGC TCCTGTTGGG
    ACCCCTAGTT TCAACAGACA ACATTTTTCT CCCCACCCTT GGACGAGCGC CTCAAACTCA
    TCCACTTCTG CCCCACCAAC ATTGGGCCAA CCAAAAGGAG GCAGTGCCAG TCAGGATCGA
    AAAATCCCTC CCCCCATTGG AACAGAGAGA CTAGCCCGAA TTCGACAAGG AGGGTCTGTC
    GCACAAGCCC CTGTGGGAAC CGGTTTTGTC GCTCCTGTTG GACACAGTGG AATCTGGTCA
    TTTGGTGTCA ATGCCATGTC AGAAGGGTTA TCAGGTTGGT CACAGTCTGT GATAGGGAAC
    CATCCAATGC ATCAACAATT GTCAGACCCA AGCACATTCT CTCAGCATCA GCCAATGGAG
    AGAGATGATT CTGGAATGGT AGCCCCCTCT AACATTTTTC ATCAGCCTAT GGCAAGCAGT
    TTTGTGGATT TTTCTAAAGG TCTACCAATT TCCATGTATG GAGGCACCAT AATACCCTCC
    CATCCTCAGC TTGCTGATGT TCCAGGAGGC CCTCTGTTTA ATGGACTTCA CAACCCAGAT
    CCTTCTTGGA ACCCTATGAT AAAAGTTATT CAAAATTCAA CTGAATGCAC TGATGCCCAG
    CAGATTTGGC CTGGCACGTG GGCACCTCAT ATTGGAAACA TGCATCTCAA ATATGTCAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.