EZH2 cDNA ORF clone, Bos taurus(cattle)

The following EZH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EZH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa47359 XM_010804543.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa18837 NM_001193024.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47356 XM_015470756.2
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47357 XM_015470757.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47358 XM_015470758.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa233603 XM_024990578.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233604 XM_024990579.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233605 XM_024990580.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233606 XM_024990581.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233607 XM_024990582.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233608 XM_024990583.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233609 XM_015470758.2
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa233610 XM_024990586.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa233610 XM_024990585.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa233611 XM_024990587.1
Latest version!
Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa47356 XM_015470756.1 Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa47359
    Clone ID Related Accession (Same CDS sequence) XM_010804543.1
    Accession Version XM_010804543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAGTTATT CTTTTCATGC AACACCCAAC
    ACTTACAAGC GGAAGAACAC AGAGACGGCC CTCGACAACA AGCCTTGTGG CCCCCACTGT
    TACCAGCACT TGGAGGGAGC GAAGGAGTTC GCTGCGGCTC TCACCGCCGA GCGGATCAAG
    ACCCCCCCGA AGCGCCCGGG GGGCCGCCGG AGAGGCCGGC TTCCCAACAA CAGCAGCAGG
    CCCAGCACCC CCACCATCAA CGTGCTGGAG TCGAAGGACA CGGACAGCGA CCGGGAGGCG
    GGGGCCGAGA CTGGCGGGGA GAGCAACGAC AAGGACGAGG AGGAGAAGAA GGACGAGACC
    TCAAGCTCCT CGGAAGCGAA TTCTCGGTGT CAAACGCCAA TAAAGATGAA GCCCAACACG
    GAGCCCCCCG AGAATGTGGA GTGGAGCGGC GCTGAGGCCT CCATGTTCAG GGTCCTCATC
    GGCACCTACT ATGACAATTT CTGTGCCATC GCCAGGCTCA TCGGGACCAA AACATGTCGA
    CAGGTGTATG AGTTTCGAGT CAAAGAATCC AGTGTCATCG CGCCGGCCCC TGCCGAGGAC
    GTGGACACTC CTCCCCGAAA GAAGAAGAGG AAACACCGGT TGTGGGCCGC ACACTGCAGA
    AAGATCCAGC TGAAGAAGGA CGGCTCCTCC AACCACGTTT ACAACTATCA ACCCTGTGAC
    CACCCGCGGC AGCCTTGCGA CAGCTCGTGC CCTTGTGTGA TAGCACAGAA TTTTTGTGAA
    AAATTTTGTC AGTGTAGTTC AGAGTGTCAG AACCGCTTTC CTGGCTGCCG CTGCAAAGCC
    CAATGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GCGAGTGCGA CCCTGACCTC
    TGTCTCACCT GCGGCGCGGC CGACCACTGG GACAGCAAGA ACGTGTCCTG CAAGAACTGC
    AGCATCCAGC GGGGCTCCAA GAAGCATTTA CTGTTGGCAC CGTCTGATGT CGCAGGCTGG
    GGGATCTTCA TCAAGGATCC TGTACAGAAA AATGAATTCA TCTCGGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC AGACAGAAGA GGGAAAGTGT ATGACAAGTA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA CGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAGATT
    CGTTTTGCAA ATCACTCAGT AAATCCCAAC TGCTATGCAA AAGTGATGAT GGTCAACGGC
    GATCACAGGA TAGGGATTTT TGCTAAGCGA GCCATCCAGA CTGGTGAAGA GCTGTTTTTC
    GATTACAGAT ACAGCCAGGC CGACGCCCTG AAGTACGTGG GCATCGAAAG AGAAATGGAG
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802845.1
    CDS439..2367
    Translation

    Target ORF information:

    RefSeq Version XM_010804543.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAGTTATT CTTTTCATGC AACACCCAAC
    ACTTACAAGC GGAAGAACAC AGAGACGGCC CTCGACAACA AGCCTTGTGG CCCCCACTGT
    TACCAGCACT TGGAGGGAGC GAAGGAGTTC GCTGCGGCTC TCACCGCCGA GCGGATCAAG
    ACCCCCCCGA AGCGCCCGGG GGGCCGCCGG AGAGGCCGGC TTCCCAACAA CAGCAGCAGG
    CCCAGCACCC CCACCATCAA CGTGCTGGAG TCGAAGGACA CGGACAGCGA CCGGGAGGCG
    GGGGCCGAGA CTGGCGGGGA GAGCAACGAC AAGGACGAGG AGGAGAAGAA GGACGAGACC
    TCAAGCTCCT CGGAAGCGAA TTCTCGGTGT CAAACGCCAA TAAAGATGAA GCCCAACACG
    GAGCCCCCCG AGAATGTGGA GTGGAGCGGC GCTGAGGCCT CCATGTTCAG GGTCCTCATC
    GGCACCTACT ATGACAATTT CTGTGCCATC GCCAGGCTCA TCGGGACCAA AACATGTCGA
    CAGGTGTATG AGTTTCGAGT CAAAGAATCC AGTGTCATCG CGCCGGCCCC TGCCGAGGAC
    GTGGACACTC CTCCCCGAAA GAAGAAGAGG AAACACCGGT TGTGGGCCGC ACACTGCAGA
    AAGATCCAGC TGAAGAAGGA CGGCTCCTCC AACCACGTTT ACAACTATCA ACCCTGTGAC
    CACCCGCGGC AGCCTTGCGA CAGCTCGTGC CCTTGTGTGA TAGCACAGAA TTTTTGTGAA
    AAATTTTGTC AGTGTAGTTC AGAGTGTCAG AACCGCTTTC CTGGCTGCCG CTGCAAAGCC
    CAATGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GCGAGTGCGA CCCTGACCTC
    TGTCTCACCT GCGGCGCGGC CGACCACTGG GACAGCAAGA ACGTGTCCTG CAAGAACTGC
    AGCATCCAGC GGGGCTCCAA GAAGCATTTA CTGTTGGCAC CGTCTGATGT CGCAGGCTGG
    GGGATCTTCA TCAAGGATCC TGTACAGAAA AATGAATTCA TCTCGGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC AGACAGAAGA GGGAAAGTGT ATGACAAGTA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA CGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAGATT
    CGTTTTGCAA ATCACTCAGT AAATCCCAAC TGCTATGCAA AAGTGATGAT GGTCAACGGC
    GATCACAGGA TAGGGATTTT TGCTAAGCGA GCCATCCAGA CTGGTGAAGA GCTGTTTTTC
    GATTACAGAT ACAGCCAGGC CGACGCCCTG AAGTACGTGG GCATCGAAAG AGAAATGGAG
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18837
    Clone ID Related Accession (Same CDS sequence) NM_001193024.1
    Accession Version NM_001193024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000004.1. On Jul 17, 2010 this sequence version replaced XM_865659.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AGTTATTCTT TTCATGCAAC ACCCAACACT TACAAGCGGA AGAACACAGA GACGGCCCTC
    GACAACAAGC CTTGTGGCCC CCACTGTTAC CAGCACTTGG AGGGAGCGAA GGAGTTCGCT
    GCGGCTCTCA CCGCCGAGCG GATCAAGACC CCCCCGAAGC GCCCGGGGGG CCGCCGGAGA
    GGCCGGCTTC CCAACAACAG CAGCAGGCCC AGCACCCCCA CCATCAACGT GCTGGAGTCG
    AAGGACACGG ACAGCGACCG GGAGGCGGGG GCCGAGACTG GCGGGGAGAG CAACGACAAG
    GACGAGGAGG AGAAGAAGGA CGAGACCTCA AGCTCCTCGG AAGCGAATTC TCGGTGTCAA
    ACGCCAATAA AGATGAAGCC CAACACGGAG CCCCCCGAGA ATGTGGAGTG GAGCGGCGCT
    GAGGCCTCCA TGTTCAGGGT CCTCATCGGC ACCTACTATG ACAATTTCTG TGCCATCGCC
    AGGCTCATCG GGACCAAAAC ATGTCGACAG GTGTATGAGT TTCGAGTCAA AGAATCCAGT
    GTCATCGCGC CGGCCCCTGC CGAGGACGTG GACACTCCTC CCCGAAAGAA GAAGAGGAAA
    CACCGGTTGT GGGCCGCACA CTGCAGAAAG ATCCAGCTGA AGAAGGACGG CTCCTCCAAC
    CACGTTTACA ACTATCAACC CTGTGACCAC CCGCGGCAGC CTTGCGACAG CTCGTGCCCT
    TGTGTGATAG CACAGAATTT TTGTGAAAAA TTTTGTCAGT GTAGTTCAGA GTGTCAGAAC
    CGCTTTCCTG GCTGCCGCTG CAAAGCCCAA TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGCG AGTGCGACCC TGACCTCTGT CTCACCTGCG GCGCGGCCGA CCACTGGGAC
    AGCAAGAACG TGTCCTGCAA GAACTGCAGC ATCCAGCGGG GCTCCAAGAA GCATTTACTG
    TTGGCACCGT CTGATGTCGC AGGCTGGGGG ATCTTCATCA AGGATCCTGT ACAGAAAAAT
    GAATTCATCT CGGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCAGA CAGAAGAGGG
    AAAGTGTATG ACAAGTACAT GTGCAGCTTT CTGTTCAACT TGAACAACGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAGATTCGT TTTGCAAATC ACTCAGTAAA TCCCAACTGC
    TATGCAAAAG TGATGATGGT CAACGGCGAT CACAGGATAG GGATTTTTGC TAAGCGAGCC
    ATCCAGACTG GTGAAGAGCT GTTTTTCGAT TACAGATACA GCCAGGCCGA CGCCCTGAAG
    TACGTGGGCA TCGAAAGAGA AATGGAGATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179953.1
    CDS2..2257
    Translation

    Target ORF information:

    RefSeq Version NM_001193024.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AGTTATTCTT TTCATGCAAC ACCCAACACT TACAAGCGGA AGAACACAGA GACGGCCCTC
    GACAACAAGC CTTGTGGCCC CCACTGTTAC CAGCACTTGG AGGGAGCGAA GGAGTTCGCT
    GCGGCTCTCA CCGCCGAGCG GATCAAGACC CCCCCGAAGC GCCCGGGGGG CCGCCGGAGA
    GGCCGGCTTC CCAACAACAG CAGCAGGCCC AGCACCCCCA CCATCAACGT GCTGGAGTCG
    AAGGACACGG ACAGCGACCG GGAGGCGGGG GCCGAGACTG GCGGGGAGAG CAACGACAAG
    GACGAGGAGG AGAAGAAGGA CGAGACCTCA AGCTCCTCGG AAGCGAATTC TCGGTGTCAA
    ACGCCAATAA AGATGAAGCC CAACACGGAG CCCCCCGAGA ATGTGGAGTG GAGCGGCGCT
    GAGGCCTCCA TGTTCAGGGT CCTCATCGGC ACCTACTATG ACAATTTCTG TGCCATCGCC
    AGGCTCATCG GGACCAAAAC ATGTCGACAG GTGTATGAGT TTCGAGTCAA AGAATCCAGT
    GTCATCGCGC CGGCCCCTGC CGAGGACGTG GACACTCCTC CCCGAAAGAA GAAGAGGAAA
    CACCGGTTGT GGGCCGCACA CTGCAGAAAG ATCCAGCTGA AGAAGGACGG CTCCTCCAAC
    CACGTTTACA ACTATCAACC CTGTGACCAC CCGCGGCAGC CTTGCGACAG CTCGTGCCCT
    TGTGTGATAG CACAGAATTT TTGTGAAAAA TTTTGTCAGT GTAGTTCAGA GTGTCAGAAC
    CGCTTTCCTG GCTGCCGCTG CAAAGCCCAA TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGCG AGTGCGACCC TGACCTCTGT CTCACCTGCG GCGCGGCCGA CCACTGGGAC
    AGCAAGAACG TGTCCTGCAA GAACTGCAGC ATCCAGCGGG GCTCCAAGAA GCATTTACTG
    TTGGCACCGT CTGATGTCGC AGGCTGGGGG ATCTTCATCA AGGATCCTGT ACAGAAAAAT
    GAATTCATCT CGGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCAGA CAGAAGAGGG
    AAAGTGTATG ACAAGTACAT GTGCAGCTTT CTGTTCAACT TGAACAACGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAGATTCGT TTTGCAAATC ACTCAGTAAA TCCCAACTGC
    TATGCAAAAG TGATGATGGT CAACGGCGAT CACAGGATAG GGATTTTTGC TAAGCGAGCC
    ATCCAGACTG GTGAAGAGCT GTTTTTCGAT TACAGATACA GCCAGGCCGA CGCCCTGAAG
    TACGTGGGCA TCGAAAGAGA AATGGAGATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47356
    Clone ID Related Accession (Same CDS sequence) XM_015470756.1 , XM_015470756.2
    Accession Version XM_015470756.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTACAA GCGGAAGAAC ACAGAGACGG CCCTCGACAA CAAGCCTTGT
    GGCCCCCACT GTTACCAGCA CTTGGAGGGA GCGAAGGAGT TCGCTGCGGC TCTCACCGCC
    GAGCGGATCA AGACCCCCCC GAAGCGCCCG GGGGGCCGCC GGAGAGGCCG GCTTCCCAAC
    AACAGCAGCA GGCCCAGCAC CCCCACCATC AACGTGCTGG AGTCGAAGGA CACGGACAGC
    GACCGGGAGG CGGGGGCCGA GACTGGCGGG GAGAGCAACG ACAAGGACGA GGAGGAGAAG
    AAGGACGAGA CCTCAAGCTC CTCGGAAGCG AATTCTCGGT GTCAAACGCC AATAAAGATG
    AAGCCCAACA CGGAGCCCCC CGAGAATGTG GAGTGGAGCG GCGCTGAGGC CTCCATGTTC
    AGGGTCCTCA TCGGCACCTA CTATGACAAT TTCTGTGCCA TCGCCAGGCT CATCGGGACC
    AAAACATGTC GACAGGTGTA TGAGTTTCGA GTCAAAGAAT CCAGTGTCAT CGCGCCGGCC
    CCTGCCGAGG ACGTGGACAC TCCTCCCCGA AAGAAGAAGA GGAAACACCG GTTGTGGGCC
    GCACACTGCA GAAAGATCCA GCTGAAGAAG GACGGCTCCT CCAACCACGT TTACAACTAT
    CAACCCTGTG ACCACCCGCG GCAGCCTTGC GACAGCTCGT GCCCTTGTGT GATAGCACAG
    AATTTTTGTG AAAAATTTTG TCAGTGTAGT TCAGAGTGTC AGAACCGCTT TCCTGGCTGC
    CGCTGCAAAG CCCAATGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGCGAGTGC
    GACCCTGACC TCTGTCTCAC CTGCGGCGCG GCCGACCACT GGGACAGCAA GAACGTGTCC
    TGCAAGAACT GCAGCATCCA GCGGGGCTCC AAGAAGCATT TACTGTTGGC ACCGTCTGAT
    GTCGCAGGCT GGGGGATCTT CATCAAGGAT CCTGTACAGA AAAATGAATT CATCTCGGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCAGACAGAA GAGGGAAAGT GTATGACAAG
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AACGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAGA TTCGTTTTGC AAATCACTCA GTAAATCCCA ACTGCTATGC AAAAGTGATG
    ATGGTCAACG GCGATCACAG GATAGGGATT TTTGCTAAGC GAGCCATCCA GACTGGTGAA
    GAGCTGTTTT TCGATTACAG ATACAGCCAG GCCGACGCCC TGAAGTACGT GGGCATCGAA
    AGAGAAATGG AGATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326242.1
    CDS8..2248
    Translation

    Target ORF information:

    RefSeq Version XM_015470756.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTACAA GCGGAAGAAC ACAGAGACGG CCCTCGACAA CAAGCCTTGT
    GGCCCCCACT GTTACCAGCA CTTGGAGGGA GCGAAGGAGT TCGCTGCGGC TCTCACCGCC
    GAGCGGATCA AGACCCCCCC GAAGCGCCCG GGGGGCCGCC GGAGAGGCCG GCTTCCCAAC
    AACAGCAGCA GGCCCAGCAC CCCCACCATC AACGTGCTGG AGTCGAAGGA CACGGACAGC
    GACCGGGAGG CGGGGGCCGA GACTGGCGGG GAGAGCAACG ACAAGGACGA GGAGGAGAAG
    AAGGACGAGA CCTCAAGCTC CTCGGAAGCG AATTCTCGGT GTCAAACGCC AATAAAGATG
    AAGCCCAACA CGGAGCCCCC CGAGAATGTG GAGTGGAGCG GCGCTGAGGC CTCCATGTTC
    AGGGTCCTCA TCGGCACCTA CTATGACAAT TTCTGTGCCA TCGCCAGGCT CATCGGGACC
    AAAACATGTC GACAGGTGTA TGAGTTTCGA GTCAAAGAAT CCAGTGTCAT CGCGCCGGCC
    CCTGCCGAGG ACGTGGACAC TCCTCCCCGA AAGAAGAAGA GGAAACACCG GTTGTGGGCC
    GCACACTGCA GAAAGATCCA GCTGAAGAAG GACGGCTCCT CCAACCACGT TTACAACTAT
    CAACCCTGTG ACCACCCGCG GCAGCCTTGC GACAGCTCGT GCCCTTGTGT GATAGCACAG
    AATTTTTGTG AAAAATTTTG TCAGTGTAGT TCAGAGTGTC AGAACCGCTT TCCTGGCTGC
    CGCTGCAAAG CCCAATGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGCGAGTGC
    GACCCTGACC TCTGTCTCAC CTGCGGCGCG GCCGACCACT GGGACAGCAA GAACGTGTCC
    TGCAAGAACT GCAGCATCCA GCGGGGCTCC AAGAAGCATT TACTGTTGGC ACCGTCTGAT
    GTCGCAGGCT GGGGGATCTT CATCAAGGAT CCTGTACAGA AAAATGAATT CATCTCGGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCAGACAGAA GAGGGAAAGT GTATGACAAG
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AACGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAGA TTCGTTTTGC AAATCACTCA GTAAATCCCA ACTGCTATGC AAAAGTGATG
    ATGGTCAACG GCGATCACAG GATAGGGATT TTTGCTAAGC GAGCCATCCA GACTGGTGAA
    GAGCTGTTTT TCGATTACAG ATACAGCCAG GCCGACGCCC TGAAGTACGT GGGCATCGAA
    AGAGAAATGG AGATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47356
    Clone ID Related Accession (Same CDS sequence) XM_015470756.1 , XM_015470756.2
    Accession Version XM_015470756.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTACAA GCGGAAGAAC ACAGAGACGG CCCTCGACAA CAAGCCTTGT
    GGCCCCCACT GTTACCAGCA CTTGGAGGGA GCGAAGGAGT TCGCTGCGGC TCTCACCGCC
    GAGCGGATCA AGACCCCCCC GAAGCGCCCG GGGGGCCGCC GGAGAGGCCG GCTTCCCAAC
    AACAGCAGCA GGCCCAGCAC CCCCACCATC AACGTGCTGG AGTCGAAGGA CACGGACAGC
    GACCGGGAGG CGGGGGCCGA GACTGGCGGG GAGAGCAACG ACAAGGACGA GGAGGAGAAG
    AAGGACGAGA CCTCAAGCTC CTCGGAAGCG AATTCTCGGT GTCAAACGCC AATAAAGATG
    AAGCCCAACA CGGAGCCCCC CGAGAATGTG GAGTGGAGCG GCGCTGAGGC CTCCATGTTC
    AGGGTCCTCA TCGGCACCTA CTATGACAAT TTCTGTGCCA TCGCCAGGCT CATCGGGACC
    AAAACATGTC GACAGGTGTA TGAGTTTCGA GTCAAAGAAT CCAGTGTCAT CGCGCCGGCC
    CCTGCCGAGG ACGTGGACAC TCCTCCCCGA AAGAAGAAGA GGAAACACCG GTTGTGGGCC
    GCACACTGCA GAAAGATCCA GCTGAAGAAG GACGGCTCCT CCAACCACGT TTACAACTAT
    CAACCCTGTG ACCACCCGCG GCAGCCTTGC GACAGCTCGT GCCCTTGTGT GATAGCACAG
    AATTTTTGTG AAAAATTTTG TCAGTGTAGT TCAGAGTGTC AGAACCGCTT TCCTGGCTGC
    CGCTGCAAAG CCCAATGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGCGAGTGC
    GACCCTGACC TCTGTCTCAC CTGCGGCGCG GCCGACCACT GGGACAGCAA GAACGTGTCC
    TGCAAGAACT GCAGCATCCA GCGGGGCTCC AAGAAGCATT TACTGTTGGC ACCGTCTGAT
    GTCGCAGGCT GGGGGATCTT CATCAAGGAT CCTGTACAGA AAAATGAATT CATCTCGGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCAGACAGAA GAGGGAAAGT GTATGACAAG
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AACGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAGA TTCGTTTTGC AAATCACTCA GTAAATCCCA ACTGCTATGC AAAAGTGATG
    ATGGTCAACG GCGATCACAG GATAGGGATT TTTGCTAAGC GAGCCATCCA GACTGGTGAA
    GAGCTGTTTT TCGATTACAG ATACAGCCAG GCCGACGCCC TGAAGTACGT GGGCATCGAA
    AGAGAAATGG AGATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326242.1
    CDS95..2335
    Translation

    Target ORF information:

    RefSeq Version XM_015470756.2
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTACAA GCGGAAGAAC ACAGAGACGG CCCTCGACAA CAAGCCTTGT
    GGCCCCCACT GTTACCAGCA CTTGGAGGGA GCGAAGGAGT TCGCTGCGGC TCTCACCGCC
    GAGCGGATCA AGACCCCCCC GAAGCGCCCG GGGGGCCGCC GGAGAGGCCG GCTTCCCAAC
    AACAGCAGCA GGCCCAGCAC CCCCACCATC AACGTGCTGG AGTCGAAGGA CACGGACAGC
    GACCGGGAGG CGGGGGCCGA GACTGGCGGG GAGAGCAACG ACAAGGACGA GGAGGAGAAG
    AAGGACGAGA CCTCAAGCTC CTCGGAAGCG AATTCTCGGT GTCAAACGCC AATAAAGATG
    AAGCCCAACA CGGAGCCCCC CGAGAATGTG GAGTGGAGCG GCGCTGAGGC CTCCATGTTC
    AGGGTCCTCA TCGGCACCTA CTATGACAAT TTCTGTGCCA TCGCCAGGCT CATCGGGACC
    AAAACATGTC GACAGGTGTA TGAGTTTCGA GTCAAAGAAT CCAGTGTCAT CGCGCCGGCC
    CCTGCCGAGG ACGTGGACAC TCCTCCCCGA AAGAAGAAGA GGAAACACCG GTTGTGGGCC
    GCACACTGCA GAAAGATCCA GCTGAAGAAG GACGGCTCCT CCAACCACGT TTACAACTAT
    CAACCCTGTG ACCACCCGCG GCAGCCTTGC GACAGCTCGT GCCCTTGTGT GATAGCACAG
    AATTTTTGTG AAAAATTTTG TCAGTGTAGT TCAGAGTGTC AGAACCGCTT TCCTGGCTGC
    CGCTGCAAAG CCCAATGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGCGAGTGC
    GACCCTGACC TCTGTCTCAC CTGCGGCGCG GCCGACCACT GGGACAGCAA GAACGTGTCC
    TGCAAGAACT GCAGCATCCA GCGGGGCTCC AAGAAGCATT TACTGTTGGC ACCGTCTGAT
    GTCGCAGGCT GGGGGATCTT CATCAAGGAT CCTGTACAGA AAAATGAATT CATCTCGGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCAGACAGAA GAGGGAAAGT GTATGACAAG
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AACGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAGA TTCGTTTTGC AAATCACTCA GTAAATCCCA ACTGCTATGC AAAAGTGATG
    ATGGTCAACG GCGATCACAG GATAGGGATT TTTGCTAAGC GAGCCATCCA GACTGGTGAA
    GAGCTGTTTT TCGATTACAG ATACAGCCAG GCCGACGCCC TGAAGTACGT GGGCATCGAA
    AGAGAAATGG AGATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47357
    Clone ID Related Accession (Same CDS sequence) XM_015470757.1
    Accession Version XM_015470757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AGTTATTCTT TTCATGCAAC ACCCAACACT TACAAGCGGA AGAACACAGA GACGGCCCTC
    GACAACAAGC CTTGTGGCCC CCACTGTTAC CAGCACTTGG AGGGAGCGAA GGAGTTCGCT
    GCGGCTCTCA CCGCCGAGCG GATCAAGACC CCCCCGAAGC GCCCGGGGGG CCGCCGGAGA
    GGCCGGCTTC CCAACAACAG CAGCAGGCCC AGCACCCCCA CCATCAACGT GCTGGAGTCG
    AAGGACACGG ACAGCGACCG GGAGGCGGGG GCCGAGACTG GCGGGGAGAG CAACGACAAG
    GACGAGGAGG AGAAGAAGGA CGAGACCTCA AGCTCCTCGG AAGCGAATTC TCGGTGTCAA
    ACGCCAATAA AGATGAAGCC CAACACGGAG CCCCCCGAGA ATGTGGAGTG GAGCGGCGCT
    GAGGCCTCCA TGTTCAGGGT CCTCATCGGC ACCTACTATG ACAATTTCTG TGCCATCGCC
    AGGCTCATCG GGACCAAAAC ATGTCGACAG GTGTATGAGT TTCGAGTCAA AGAATCCAGT
    GTCATCGCGC CGGCCCCTGC CGAGGACGTG GACACTCCTC CCCGAAAGAA GAAGAGGAAA
    CACCGGTTGT GGGCCGCACA CTGCAGAAAG ATCCAGCTGA AGAAGGGTCA GAACCGCTTT
    CCTGGCTGCC GCTGCAAAGC CCAATGCAAC ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC
    CGCGAGTGCG ACCCTGACCT CTGTCTCACC TGCGGCGCGG CCGACCACTG GGACAGCAAG
    AACGTGTCCT GCAAGAACTG CAGCATCCAG CGGGGCTCCA AGAAGCATTT ACTGTTGGCA
    CCGTCTGATG TCGCAGGCTG GGGGATCTTC ATCAAGGATC CTGTACAGAA AAATGAATTC
    ATCTCGGAAT ACTGTGGAGA GATTATTTCT CAAGATGAAG CAGACAGAAG AGGGAAAGTG
    TATGACAAGT ACATGTGCAG CTTTCTGTTC AACTTGAACA ACGATTTTGT GGTGGATGCA
    ACCCGCAAGG GTAACAAGAT TCGTTTTGCA AATCACTCAG TAAATCCCAA CTGCTATGCA
    AAAGTGATGA TGGTCAACGG CGATCACAGG ATAGGGATTT TTGCTAAGCG AGCCATCCAG
    ACTGGTGAAG AGCTGTTTTT CGATTACAGA TACAGCCAGG CCGACGCCCT GAAGTACGTG
    GGCATCGAAA GAGAAATGGA GATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326243.1
    CDS8..2137
    Translation

    Target ORF information:

    RefSeq Version XM_015470757.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AGTTATTCTT TTCATGCAAC ACCCAACACT TACAAGCGGA AGAACACAGA GACGGCCCTC
    GACAACAAGC CTTGTGGCCC CCACTGTTAC CAGCACTTGG AGGGAGCGAA GGAGTTCGCT
    GCGGCTCTCA CCGCCGAGCG GATCAAGACC CCCCCGAAGC GCCCGGGGGG CCGCCGGAGA
    GGCCGGCTTC CCAACAACAG CAGCAGGCCC AGCACCCCCA CCATCAACGT GCTGGAGTCG
    AAGGACACGG ACAGCGACCG GGAGGCGGGG GCCGAGACTG GCGGGGAGAG CAACGACAAG
    GACGAGGAGG AGAAGAAGGA CGAGACCTCA AGCTCCTCGG AAGCGAATTC TCGGTGTCAA
    ACGCCAATAA AGATGAAGCC CAACACGGAG CCCCCCGAGA ATGTGGAGTG GAGCGGCGCT
    GAGGCCTCCA TGTTCAGGGT CCTCATCGGC ACCTACTATG ACAATTTCTG TGCCATCGCC
    AGGCTCATCG GGACCAAAAC ATGTCGACAG GTGTATGAGT TTCGAGTCAA AGAATCCAGT
    GTCATCGCGC CGGCCCCTGC CGAGGACGTG GACACTCCTC CCCGAAAGAA GAAGAGGAAA
    CACCGGTTGT GGGCCGCACA CTGCAGAAAG ATCCAGCTGA AGAAGGGTCA GAACCGCTTT
    CCTGGCTGCC GCTGCAAAGC CCAATGCAAC ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC
    CGCGAGTGCG ACCCTGACCT CTGTCTCACC TGCGGCGCGG CCGACCACTG GGACAGCAAG
    AACGTGTCCT GCAAGAACTG CAGCATCCAG CGGGGCTCCA AGAAGCATTT ACTGTTGGCA
    CCGTCTGATG TCGCAGGCTG GGGGATCTTC ATCAAGGATC CTGTACAGAA AAATGAATTC
    ATCTCGGAAT ACTGTGGAGA GATTATTTCT CAAGATGAAG CAGACAGAAG AGGGAAAGTG
    TATGACAAGT ACATGTGCAG CTTTCTGTTC AACTTGAACA ACGATTTTGT GGTGGATGCA
    ACCCGCAAGG GTAACAAGAT TCGTTTTGCA AATCACTCAG TAAATCCCAA CTGCTATGCA
    AAAGTGATGA TGGTCAACGG CGATCACAGG ATAGGGATTT TTGCTAAGCG AGCCATCCAG
    ACTGGTGAAG AGCTGTTTTT CGATTACAGA TACAGCCAGG CCGACGCCCT GAAGTACGTG
    GGCATCGAAA GAGAAATGGA GATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47358
    Clone ID Related Accession (Same CDS sequence) XM_015470758.1
    Accession Version XM_015470758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTACAA GCGGAAGAAC ACAGAGACGG CCCTCGACAA CAAGCCTTGT
    GGCCCCCACT GTTACCAGCA CTTGGAGGGA GCGAAGGAGT TCGCTGCGGC TCTCACCGCC
    GAGCGGATCA AGACCCCCCC GAAGCGCCCG GGGGGCCGCC GGAGAGGCCG GCTTCCCAAC
    AACAGCAGCA GGCCCAGCAC CCCCACCATC AACGTGCTGG AGTCGAAGGA CACGGACAGC
    GACCGGGAGG CGGGGGCCGA GACTGGCGGG GAGAGCAACG ACAAGGACGA GGAGGAGAAG
    AAGGACGAGA CCTCAAGCTC CTCGGAAGCG AATTCTCGGT GTCAAACGCC AATAAAGATG
    AAGCCCAACA CGGAGCCCCC CGAGAATGTG GAGTGGAGCG GCGCTGAGGC CTCCATGTTC
    AGGGTCCTCA TCGGCACCTA CTATGACAAT TTCTGTGCCA TCGCCAGGCT CATCGGGACC
    AAAACATGTC GACAGGTGTA TGAGTTTCGA GTCAAAGAAT CCAGTGTCAT CGCGCCGGCC
    CCTGCCGAGG ACGTGGACAC TCCTCCCCGA AAGAAGAAGA GGAAACACCG GTTGTGGGCC
    GCACACTGCA GAAAGATCCA GCTGAAGAAG GGTCAGAACC GCTTTCCTGG CTGCCGCTGC
    AAAGCCCAAT GCAACACCAA GCAGTGCCCG TGCTACCTGG CTGTCCGCGA GTGCGACCCT
    GACCTCTGTC TCACCTGCGG CGCGGCCGAC CACTGGGACA GCAAGAACGT GTCCTGCAAG
    AACTGCAGCA TCCAGCGGGG CTCCAAGAAG CATTTACTGT TGGCACCGTC TGATGTCGCA
    GGCTGGGGGA TCTTCATCAA GGATCCTGTA CAGAAAAATG AATTCATCTC GGAATACTGT
    GGAGAGATTA TTTCTCAAGA TGAAGCAGAC AGAAGAGGGA AAGTGTATGA CAAGTACATG
    TGCAGCTTTC TGTTCAACTT GAACAACGAT TTTGTGGTGG ATGCAACCCG CAAGGGTAAC
    AAGATTCGTT TTGCAAATCA CTCAGTAAAT CCCAACTGCT ATGCAAAAGT GATGATGGTC
    AACGGCGATC ACAGGATAGG GATTTTTGCT AAGCGAGCCA TCCAGACTGG TGAAGAGCTG
    TTTTTCGATT ACAGATACAG CCAGGCCGAC GCCCTGAAGT ACGTGGGCAT CGAAAGAGAA
    ATGGAGATCC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326244.1
    CDS8..2122
    Translation

    Target ORF information:

    RefSeq Version XM_015470758.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGATAAA GAAAGCCGTC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCTAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAGCTA
    AAGGAAAAGT ACAAAGAACT CACGGAGCAG CAGCTCCCAG GTGCGCTTCC TCCTGAGTGC
    ACCCCCAACA TAGATGGACC AAACGCCAAG TCTGTCCAGA GGGAGCAGAG CTTGCATTCG
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTACAA GCGGAAGAAC ACAGAGACGG CCCTCGACAA CAAGCCTTGT
    GGCCCCCACT GTTACCAGCA CTTGGAGGGA GCGAAGGAGT TCGCTGCGGC TCTCACCGCC
    GAGCGGATCA AGACCCCCCC GAAGCGCCCG GGGGGCCGCC GGAGAGGCCG GCTTCCCAAC
    AACAGCAGCA GGCCCAGCAC CCCCACCATC AACGTGCTGG AGTCGAAGGA CACGGACAGC
    GACCGGGAGG CGGGGGCCGA GACTGGCGGG GAGAGCAACG ACAAGGACGA GGAGGAGAAG
    AAGGACGAGA CCTCAAGCTC CTCGGAAGCG AATTCTCGGT GTCAAACGCC AATAAAGATG
    AAGCCCAACA CGGAGCCCCC CGAGAATGTG GAGTGGAGCG GCGCTGAGGC CTCCATGTTC
    AGGGTCCTCA TCGGCACCTA CTATGACAAT TTCTGTGCCA TCGCCAGGCT CATCGGGACC
    AAAACATGTC GACAGGTGTA TGAGTTTCGA GTCAAAGAAT CCAGTGTCAT CGCGCCGGCC
    CCTGCCGAGG ACGTGGACAC TCCTCCCCGA AAGAAGAAGA GGAAACACCG GTTGTGGGCC
    GCACACTGCA GAAAGATCCA GCTGAAGAAG GGTCAGAACC GCTTTCCTGG CTGCCGCTGC
    AAAGCCCAAT GCAACACCAA GCAGTGCCCG TGCTACCTGG CTGTCCGCGA GTGCGACCCT
    GACCTCTGTC TCACCTGCGG CGCGGCCGAC CACTGGGACA GCAAGAACGT GTCCTGCAAG
    AACTGCAGCA TCCAGCGGGG CTCCAAGAAG CATTTACTGT TGGCACCGTC TGATGTCGCA
    GGCTGGGGGA TCTTCATCAA GGATCCTGTA CAGAAAAATG AATTCATCTC GGAATACTGT
    GGAGAGATTA TTTCTCAAGA TGAAGCAGAC AGAAGAGGGA AAGTGTATGA CAAGTACATG
    TGCAGCTTTC TGTTCAACTT GAACAACGAT TTTGTGGTGG ATGCAACCCG CAAGGGTAAC
    AAGATTCGTT TTGCAAATCA CTCAGTAAAT CCCAACTGCT ATGCAAAAGT GATGATGGTC
    AACGGCGATC ACAGGATAGG GATTTTTGCT AAGCGAGCCA TCCAGACTGG TGAAGAGCTG
    TTTTTCGATT ACAGATACAG CCAGGCCGAC GCCCTGAAGT ACGTGGGCAT CGAAAGAGAA
    ATGGAGATCC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233603
    Clone ID Related Accession (Same CDS sequence) XM_024990578.1
    Accession Version XM_024990578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGACG GCTCCTCCAA CCACGTTTAC
    AACTATCAAC CCTGTGACCA CCCGCGGCAG CCTTGCGACA GCTCGTGCCC TTGTGTGATA
    GCACAGAATT TTTGTGAAAA ATTTTGTCAG TGTAGTTCAG AGTGTCAGAA CCGCTTTCCT
    GGCTGCCGCT GCAAAGCCCA ATGCAACACC AAGCAGTGCC CGTGCTACCT GGCTGTCCGC
    GAGTGCGACC CTGACCTCTG TCTCACCTGC GGCGCGGCCG ACCACTGGGA CAGCAAGAAC
    GTGTCCTGCA AGAACTGCAG CATCCAGCGG GGCTCCAAGA AGCATTTACT GTTGGCACCG
    TCTGATGTCG CAGGCTGGGG GATCTTCATC AAGGATCCTG TACAGAAAAA TGAATTCATC
    TCGGAATACT GTGGAGAGAT TATTTCTCAA GATGAAGCAG ACAGAAGAGG GAAAGTGTAT
    GACAAGTACA TGTGCAGCTT TCTGTTCAAC TTGAACAACG ATTTTGTGGT GGATGCAACC
    CGCAAGGGTA ACAAGATTCG TTTTGCAAAT CACTCAGTAA ATCCCAACTG CTATGCAAAA
    GTGATGATGG TCAACGGCGA TCACAGGATA GGGATTTTTG CTAAGCGAGC CATCCAGACT
    GGTGAAGAGC TGTTTTTCGA TTACAGATAC AGCCAGGCCG ACGCCCTGAA GTACGTGGGC
    ATCGAAAGAG AAATGGAGAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846346.1
    CDS131..2437
    Translation

    Target ORF information:

    RefSeq Version XM_024990578.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGACG GCTCCTCCAA CCACGTTTAC
    AACTATCAAC CCTGTGACCA CCCGCGGCAG CCTTGCGACA GCTCGTGCCC TTGTGTGATA
    GCACAGAATT TTTGTGAAAA ATTTTGTCAG TGTAGTTCAG AGTGTCAGAA CCGCTTTCCT
    GGCTGCCGCT GCAAAGCCCA ATGCAACACC AAGCAGTGCC CGTGCTACCT GGCTGTCCGC
    GAGTGCGACC CTGACCTCTG TCTCACCTGC GGCGCGGCCG ACCACTGGGA CAGCAAGAAC
    GTGTCCTGCA AGAACTGCAG CATCCAGCGG GGCTCCAAGA AGCATTTACT GTTGGCACCG
    TCTGATGTCG CAGGCTGGGG GATCTTCATC AAGGATCCTG TACAGAAAAA TGAATTCATC
    TCGGAATACT GTGGAGAGAT TATTTCTCAA GATGAAGCAG ACAGAAGAGG GAAAGTGTAT
    GACAAGTACA TGTGCAGCTT TCTGTTCAAC TTGAACAACG ATTTTGTGGT GGATGCAACC
    CGCAAGGGTA ACAAGATTCG TTTTGCAAAT CACTCAGTAA ATCCCAACTG CTATGCAAAA
    GTGATGATGG TCAACGGCGA TCACAGGATA GGGATTTTTG CTAAGCGAGC CATCCAGACT
    GGTGAAGAGC TGTTTTTCGA TTACAGATAC AGCCAGGCCG ACGCCCTGAA GTACGTGGGC
    ATCGAAAGAG AAATGGAGAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233604
    Clone ID Related Accession (Same CDS sequence) XM_024990579.1
    Accession Version XM_024990579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCCTTTTCA TGCAACACCC
    AACACTTACA AGCGGAAGAA CACAGAGACG GCCCTCGACA ACAAGCCTTG TGGCCCCCAC
    TGTTACCAGC ACTTGGAGGG AGCGAAGGAG TTCGCTGCGG CTCTCACCGC CGAGCGGATC
    AAGACCCCCC CGAAGCGCCC GGGGGGCCGC CGGAGAGGCC GGCTTCCCAA CAACAGCAGC
    AGGCCCAGCA CCCCCACCAT CAACGTGCTG GAGTCGAAGG ACACGGACAG CGACCGGGAG
    GCGGGGGCCG AGACTGGCGG GGAGAGCAAC GACAAGGACG AGGAGGAGAA GAAGGACGAG
    ACCTCAAGCT CCTCGGAAGC GAATTCTCGG TGTCAAACGC CAATAAAGAT GAAGCCCAAC
    ACGGAGCCCC CCGAGAATGT GGAGTGGAGC GGCGCTGAGG CCTCCATGTT CAGGGTCCTC
    ATCGGCACCT ACTATGACAA TTTCTGTGCC ATCGCCAGGC TCATCGGGAC CAAAACATGT
    CGACAGGTGT ATGAGTTTCG AGTCAAAGAA TCCAGTGTCA TCGCGCCGGC CCCTGCCGAG
    GACGTGGACA CTCCTCCCCG AAAGAAGAAG AGGAAACACC GGTTGTGGGC CGCACACTGC
    AGAAAGATCC AGCTGAAGAA GGACGGCTCC TCCAACCACG TTTACAACTA TCAACCCTGT
    GACCACCCGC GGCAGCCTTG CGACAGCTCG TGCCCTTGTG TGATAGCACA GAATTTTTGT
    GAAAAATTTT GTCAGTGTAG TTCAGAGTGT CAGAACCGCT TTCCTGGCTG CCGCTGCAAA
    GCCCAATGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGCGAGTG CGACCCTGAC
    CTCTGTCTCA CCTGCGGCGC GGCCGACCAC TGGGACAGCA AGAACGTGTC CTGCAAGAAC
    TGCAGCATCC AGCGGGGCTC CAAGAAGCAT TTACTGTTGG CACCGTCTGA TGTCGCAGGC
    TGGGGGATCT TCATCAAGGA TCCTGTACAG AAAAATGAAT TCATCTCGGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCAGACAGA AGAGGGAAAG TGTATGACAA GTACATGTGC
    AGCTTTCTGT TCAACTTGAA CAACGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAG
    ATTCGTTTTG CAAATCACTC AGTAAATCCC AACTGCTATG CAAAAGTGAT GATGGTCAAC
    GGCGATCACA GGATAGGGAT TTTTGCTAAG CGAGCCATCC AGACTGGTGA AGAGCTGTTT
    TTCGATTACA GATACAGCCA GGCCGACGCC CTGAAGTACG TGGGCATCGA AAGAGAAATG
    GAGATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846347.1
    CDS129..2420
    Translation

    Target ORF information:

    RefSeq Version XM_024990579.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCCTTTTCA TGCAACACCC
    AACACTTACA AGCGGAAGAA CACAGAGACG GCCCTCGACA ACAAGCCTTG TGGCCCCCAC
    TGTTACCAGC ACTTGGAGGG AGCGAAGGAG TTCGCTGCGG CTCTCACCGC CGAGCGGATC
    AAGACCCCCC CGAAGCGCCC GGGGGGCCGC CGGAGAGGCC GGCTTCCCAA CAACAGCAGC
    AGGCCCAGCA CCCCCACCAT CAACGTGCTG GAGTCGAAGG ACACGGACAG CGACCGGGAG
    GCGGGGGCCG AGACTGGCGG GGAGAGCAAC GACAAGGACG AGGAGGAGAA GAAGGACGAG
    ACCTCAAGCT CCTCGGAAGC GAATTCTCGG TGTCAAACGC CAATAAAGAT GAAGCCCAAC
    ACGGAGCCCC CCGAGAATGT GGAGTGGAGC GGCGCTGAGG CCTCCATGTT CAGGGTCCTC
    ATCGGCACCT ACTATGACAA TTTCTGTGCC ATCGCCAGGC TCATCGGGAC CAAAACATGT
    CGACAGGTGT ATGAGTTTCG AGTCAAAGAA TCCAGTGTCA TCGCGCCGGC CCCTGCCGAG
    GACGTGGACA CTCCTCCCCG AAAGAAGAAG AGGAAACACC GGTTGTGGGC CGCACACTGC
    AGAAAGATCC AGCTGAAGAA GGACGGCTCC TCCAACCACG TTTACAACTA TCAACCCTGT
    GACCACCCGC GGCAGCCTTG CGACAGCTCG TGCCCTTGTG TGATAGCACA GAATTTTTGT
    GAAAAATTTT GTCAGTGTAG TTCAGAGTGT CAGAACCGCT TTCCTGGCTG CCGCTGCAAA
    GCCCAATGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGCGAGTG CGACCCTGAC
    CTCTGTCTCA CCTGCGGCGC GGCCGACCAC TGGGACAGCA AGAACGTGTC CTGCAAGAAC
    TGCAGCATCC AGCGGGGCTC CAAGAAGCAT TTACTGTTGG CACCGTCTGA TGTCGCAGGC
    TGGGGGATCT TCATCAAGGA TCCTGTACAG AAAAATGAAT TCATCTCGGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCAGACAGA AGAGGGAAAG TGTATGACAA GTACATGTGC
    AGCTTTCTGT TCAACTTGAA CAACGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAG
    ATTCGTTTTG CAAATCACTC AGTAAATCCC AACTGCTATG CAAAAGTGAT GATGGTCAAC
    GGCGATCACA GGATAGGGAT TTTTGCTAAG CGAGCCATCC AGACTGGTGA AGAGCTGTTT
    TTCGATTACA GATACAGCCA GGCCGACGCC CTGAAGTACG TGGGCATCGA AAGAGAAATG
    GAGATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233605
    Clone ID Related Accession (Same CDS sequence) XM_024990580.1
    Accession Version XM_024990580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAGTTATT CTTTTCATGC AACACCCAAC
    ACTTACAAGC GGAAGAACAC AGAGACGGCC CTCGACAACA AGCCTTGTGG CCCCCACTGT
    TACCAGCACT TGGAGGGAGC GAAGGAGTTC GCTGCGGCTC TCACCGCCGA GCGGATCAAG
    ACCCCCCCGA AGCGCCCGGG GGGCCGCCGG AGAGGCCGGC TTCCCAACAA CAGCAGCAGG
    CCCAGCACCC CCACCATCAA CGTGCTGGAG TCGAAGGACA CGGACAGCGA CCGGGAGGCG
    GGGGCCGAGA CTGGCGGGGA GAGCAACGAC AAGGACGAGG AGGAGAAGAA GGACGAGACC
    TCAAGCTCCT CGGAAGCGAA TTCTCGGTGT CAAACGCCAA TAAAGATGAA GCCCAACACG
    GAGCCCCCCG AGAATGTGGA GTGGAGCGGC GCTGAGGCCT CCATGTTCAG GGTCCTCATC
    GGCACCTACT ATGACAATTT CTGTGCCATC GCCAGGCTCA TCGGGACCAA AACATGTCGA
    CAGGTGTATG AGTTTCGAGT CAAAGAATCC AGTGTCATCG CGCCGGCCCC TGCCGAGGAC
    GTGGACACTC CTCCCCGAAA GAAGAAGAGG AAACACCGGT TGTGGGCCGC ACACTGCAGA
    AAGATCCAGC TGAAGAAGGA CGGCTCCTCC AACCACGTTT ACAACTATCA ACCCTGTGAC
    CACCCGCGGC AGCCTTGCGA CAGCTCGTGC CCTTGTGTGA TAGCACAGAA TTTTTGTGAA
    AAATTTTGTC AGTGTAGTTC AGAGTGTCAG AACCGCTTTC CTGGCTGCCG CTGCAAAGCC
    CAATGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GCGAGTGCGA CCCTGACCTC
    TGTCTCACCT GCGGCGCGGC CGACCACTGG GACAGCAAGA ACGTGTCCTG CAAGAACTGC
    AGCATCCAGC GGGGCTCCAA GAAGCATTTA CTGTTGGCAC CGTCTGATGT CGCAGGCTGG
    GGGATCTTCA TCAAGGATCC TGTACAGAAA AATGAATTCA TCTCGGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC AGACAGAAGA GGGAAAGTGT ATGACAAGTA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA CGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAGATT
    CGTTTTGCAA ATCACTCAGT AAATCCCAAC TGCTATGCAA AAGTGATGAT GGTCAACGGC
    GATCACAGGA TAGGGATTTT TGCTAAGCGA GCCATCCAGA CTGGTGAAGA GCTGTTTTTC
    GATTACAGAT ACAGCCAGGC CGACGCCCTG AAGTACGTGG GCATCGAAAG AGAAATGGAG
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846348.1
    CDS128..2416
    Translation

    Target ORF information:

    RefSeq Version XM_024990580.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAGTTATT CTTTTCATGC AACACCCAAC
    ACTTACAAGC GGAAGAACAC AGAGACGGCC CTCGACAACA AGCCTTGTGG CCCCCACTGT
    TACCAGCACT TGGAGGGAGC GAAGGAGTTC GCTGCGGCTC TCACCGCCGA GCGGATCAAG
    ACCCCCCCGA AGCGCCCGGG GGGCCGCCGG AGAGGCCGGC TTCCCAACAA CAGCAGCAGG
    CCCAGCACCC CCACCATCAA CGTGCTGGAG TCGAAGGACA CGGACAGCGA CCGGGAGGCG
    GGGGCCGAGA CTGGCGGGGA GAGCAACGAC AAGGACGAGG AGGAGAAGAA GGACGAGACC
    TCAAGCTCCT CGGAAGCGAA TTCTCGGTGT CAAACGCCAA TAAAGATGAA GCCCAACACG
    GAGCCCCCCG AGAATGTGGA GTGGAGCGGC GCTGAGGCCT CCATGTTCAG GGTCCTCATC
    GGCACCTACT ATGACAATTT CTGTGCCATC GCCAGGCTCA TCGGGACCAA AACATGTCGA
    CAGGTGTATG AGTTTCGAGT CAAAGAATCC AGTGTCATCG CGCCGGCCCC TGCCGAGGAC
    GTGGACACTC CTCCCCGAAA GAAGAAGAGG AAACACCGGT TGTGGGCCGC ACACTGCAGA
    AAGATCCAGC TGAAGAAGGA CGGCTCCTCC AACCACGTTT ACAACTATCA ACCCTGTGAC
    CACCCGCGGC AGCCTTGCGA CAGCTCGTGC CCTTGTGTGA TAGCACAGAA TTTTTGTGAA
    AAATTTTGTC AGTGTAGTTC AGAGTGTCAG AACCGCTTTC CTGGCTGCCG CTGCAAAGCC
    CAATGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GCGAGTGCGA CCCTGACCTC
    TGTCTCACCT GCGGCGCGGC CGACCACTGG GACAGCAAGA ACGTGTCCTG CAAGAACTGC
    AGCATCCAGC GGGGCTCCAA GAAGCATTTA CTGTTGGCAC CGTCTGATGT CGCAGGCTGG
    GGGATCTTCA TCAAGGATCC TGTACAGAAA AATGAATTCA TCTCGGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC AGACAGAAGA GGGAAAGTGT ATGACAAGTA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA CGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAGATT
    CGTTTTGCAA ATCACTCAGT AAATCCCAAC TGCTATGCAA AAGTGATGAT GGTCAACGGC
    GATCACAGGA TAGGGATTTT TGCTAAGCGA GCCATCCAGA CTGGTGAAGA GCTGTTTTTC
    GATTACAGAT ACAGCCAGGC CGACGCCCTG AAGTACGTGG GCATCGAAAG AGAAATGGAG
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233606
    Clone ID Related Accession (Same CDS sequence) XM_024990581.1
    Accession Version XM_024990581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGACGGCT CCTCCAACCA CGTTTACAAC TATCAACCCT GTGACCACCC GCGGCAGCCT
    TGCGACAGCT CGTGCCCTTG TGTGATAGCA CAGAATTTTT GTGAAAAATT TTGTCAGTGT
    AGTTCAGAGT GTCAGAACCG CTTTCCTGGC TGCCGCTGCA AAGCCCAATG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGCGAG TGCGACCCTG ACCTCTGTCT CACCTGCGGC
    GCGGCCGACC ACTGGGACAG CAAGAACGTG TCCTGCAAGA ACTGCAGCAT CCAGCGGGGC
    TCCAAGAAGC ATTTACTGTT GGCACCGTCT GATGTCGCAG GCTGGGGGAT CTTCATCAAG
    GATCCTGTAC AGAAAAATGA ATTCATCTCG GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCAGACA GAAGAGGGAA AGTGTATGAC AAGTACATGT GCAGCTTTCT GTTCAACTTG
    AACAACGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AGATTCGTTT TGCAAATCAC
    TCAGTAAATC CCAACTGCTA TGCAAAAGTG ATGATGGTCA ACGGCGATCA CAGGATAGGG
    ATTTTTGCTA AGCGAGCCAT CCAGACTGGT GAAGAGCTGT TTTTCGATTA CAGATACAGC
    CAGGCCGACG CCCTGAAGTA CGTGGGCATC GAAAGAGAAA TGGAGATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846349.1
    CDS126..2399
    Translation

    Target ORF information:

    RefSeq Version XM_024990581.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGACGGCT CCTCCAACCA CGTTTACAAC TATCAACCCT GTGACCACCC GCGGCAGCCT
    TGCGACAGCT CGTGCCCTTG TGTGATAGCA CAGAATTTTT GTGAAAAATT TTGTCAGTGT
    AGTTCAGAGT GTCAGAACCG CTTTCCTGGC TGCCGCTGCA AAGCCCAATG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGCGAG TGCGACCCTG ACCTCTGTCT CACCTGCGGC
    GCGGCCGACC ACTGGGACAG CAAGAACGTG TCCTGCAAGA ACTGCAGCAT CCAGCGGGGC
    TCCAAGAAGC ATTTACTGTT GGCACCGTCT GATGTCGCAG GCTGGGGGAT CTTCATCAAG
    GATCCTGTAC AGAAAAATGA ATTCATCTCG GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCAGACA GAAGAGGGAA AGTGTATGAC AAGTACATGT GCAGCTTTCT GTTCAACTTG
    AACAACGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AGATTCGTTT TGCAAATCAC
    TCAGTAAATC CCAACTGCTA TGCAAAAGTG ATGATGGTCA ACGGCGATCA CAGGATAGGG
    ATTTTTGCTA AGCGAGCCAT CCAGACTGGT GAAGAGCTGT TTTTCGATTA CAGATACAGC
    CAGGCCGACG CCCTGAAGTA CGTGGGCATC GAAAGAGAAA TGGAGATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233607
    Clone ID Related Accession (Same CDS sequence) XM_024990582.1
    Accession Version XM_024990582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGGTACC TCTGTCTTGT TAGATAAAGA AAGCCGTCCA
    CCTCGGAAAT TTCCTTCTGA TAAAATTTTT GAAGCTATTT CCTCAATGTT TCCAGATAAG
    GGCACAGCAG AAGAGCTAAA GGAAAAGTAC AAAGAACTCA CGGAGCAGCA GCTCCCAGGT
    GCGCTTCCTC CTGAGTGCAC CCCCAACATA GATGGACCAA ACGCCAAGTC TGTCCAGAGG
    GAGCAGAGCT TGCATTCGTT TCATACGCTT TTCTGTAGGC GATGTTTTAA ATATGACTGC
    TTCCTACATC GTAAGTGCAG TTATTCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGACGGCT CCTCCAACCA CGTTTACAAC TATCAACCCT GTGACCACCC GCGGCAGCCT
    TGCGACAGCT CGTGCCCTTG TGTGATAGCA CAGAATTTTT GTGAAAAATT TTGTCAGTGT
    AGTTCAGAGT GTCAGAACCG CTTTCCTGGC TGCCGCTGCA AAGCCCAATG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGCGAG TGCGACCCTG ACCTCTGTCT CACCTGCGGC
    GCGGCCGACC ACTGGGACAG CAAGAACGTG TCCTGCAAGA ACTGCAGCAT CCAGCGGGGC
    TCCAAGAAGC ATTTACTGTT GGCACCGTCT GATGTCGCAG GCTGGGGGAT CTTCATCAAG
    GATCCTGTAC AGAAAAATGA ATTCATCTCG GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCAGACA GAAGAGGGAA AGTGTATGAC AAGTACATGT GCAGCTTTCT GTTCAACTTG
    AACAACGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AGATTCGTTT TGCAAATCAC
    TCAGTAAATC CCAACTGCTA TGCAAAAGTG ATGATGGTCA ACGGCGATCA CAGGATAGGG
    ATTTTTGCTA AGCGAGCCAT CCAGACTGGT GAAGAGCTGT TTTTCGATTA CAGATACAGC
    CAGGCCGACG CCCTGAAGTA CGTGGGCATC GAAAGAGAAA TGGAGATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846350.1
    CDS104..2377
    Translation

    Target ORF information:

    RefSeq Version XM_024990582.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA GTCTGAGAAG GGACCAGTGT GTTGGCGGAA GCGCGTAAAG 
    TCAGAGTACA TGCGCCTGCG GCAGCTCAAG AGATTCAGGC GCGCTGACGA AGTCAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACAG AAATCTTAAA CCAAGAGTGG
    AAACAGCGAA GAATCCAGCC TGTGCATATC CTGACTTCTG TGAGCTCATT GCGCGGGACC
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCGG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCTCTACA GCAGAATTTC
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCATACA TGGGGGATGA AGTTTTAGAC
    CAGGATGGGA CGTTCATTGA AGAACTAATA AAAAATTATG ATGGAAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGC TGGTGAATGC TCTTGGTCAG
    TACAATGATG ATGATGACGA TGATGACGGA GATGATCCTG ATGAAAGAGA AGAAAAACAG
    AAAGATCTAG AGGAGAGTCG AGAAGGTACC TCTGTCTTGT TAGATAAAGA AAGCCGTCCA
    CCTCGGAAAT TTCCTTCTGA TAAAATTTTT GAAGCTATTT CCTCAATGTT TCCAGATAAG
    GGCACAGCAG AAGAGCTAAA GGAAAAGTAC AAAGAACTCA CGGAGCAGCA GCTCCCAGGT
    GCGCTTCCTC CTGAGTGCAC CCCCAACATA GATGGACCAA ACGCCAAGTC TGTCCAGAGG
    GAGCAGAGCT TGCATTCGTT TCATACGCTT TTCTGTAGGC GATGTTTTAA ATATGACTGC
    TTCCTACATC GTAAGTGCAG TTATTCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGACGGCT CCTCCAACCA CGTTTACAAC TATCAACCCT GTGACCACCC GCGGCAGCCT
    TGCGACAGCT CGTGCCCTTG TGTGATAGCA CAGAATTTTT GTGAAAAATT TTGTCAGTGT
    AGTTCAGAGT GTCAGAACCG CTTTCCTGGC TGCCGCTGCA AAGCCCAATG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGCGAG TGCGACCCTG ACCTCTGTCT CACCTGCGGC
    GCGGCCGACC ACTGGGACAG CAAGAACGTG TCCTGCAAGA ACTGCAGCAT CCAGCGGGGC
    TCCAAGAAGC ATTTACTGTT GGCACCGTCT GATGTCGCAG GCTGGGGGAT CTTCATCAAG
    GATCCTGTAC AGAAAAATGA ATTCATCTCG GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCAGACA GAAGAGGGAA AGTGTATGAC AAGTACATGT GCAGCTTTCT GTTCAACTTG
    AACAACGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AGATTCGTTT TGCAAATCAC
    TCAGTAAATC CCAACTGCTA TGCAAAAGTG ATGATGGTCA ACGGCGATCA CAGGATAGGG
    ATTTTTGCTA AGCGAGCCAT CCAGACTGGT GAAGAGCTGT TTTTCGATTA CAGATACAGC
    CAGGCCGACG CCCTGAAGTA CGTGGGCATC GAAAGAGAAA TGGAGATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233608
    Clone ID Related Accession (Same CDS sequence) XM_024990583.1
    Accession Version XM_024990583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGGTC AGAACCGCTT TCCTGGCTGC
    CGCTGCAAAG CCCAATGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGCGAGTGC
    GACCCTGACC TCTGTCTCAC CTGCGGCGCG GCCGACCACT GGGACAGCAA GAACGTGTCC
    TGCAAGAACT GCAGCATCCA GCGGGGCTCC AAGAAGCATT TACTGTTGGC ACCGTCTGAT
    GTCGCAGGCT GGGGGATCTT CATCAAGGAT CCTGTACAGA AAAATGAATT CATCTCGGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCAGACAGAA GAGGGAAAGT GTATGACAAG
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AACGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAGA TTCGTTTTGC AAATCACTCA GTAAATCCCA ACTGCTATGC AAAAGTGATG
    ATGGTCAACG GCGATCACAG GATAGGGATT TTTGCTAAGC GAGCCATCCA GACTGGTGAA
    GAGCTGTTTT TCGATTACAG ATACAGCCAG GCCGACGCCC TGAAGTACGT GGGCATCGAA
    AGAGAAATGG AGATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846351.1
    CDS132..2312
    Translation

    Target ORF information:

    RefSeq Version XM_024990583.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGGTC AGAACCGCTT TCCTGGCTGC
    CGCTGCAAAG CCCAATGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGCGAGTGC
    GACCCTGACC TCTGTCTCAC CTGCGGCGCG GCCGACCACT GGGACAGCAA GAACGTGTCC
    TGCAAGAACT GCAGCATCCA GCGGGGCTCC AAGAAGCATT TACTGTTGGC ACCGTCTGAT
    GTCGCAGGCT GGGGGATCTT CATCAAGGAT CCTGTACAGA AAAATGAATT CATCTCGGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCAGACAGAA GAGGGAAAGT GTATGACAAG
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AACGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAGA TTCGTTTTGC AAATCACTCA GTAAATCCCA ACTGCTATGC AAAAGTGATG
    ATGGTCAACG GCGATCACAG GATAGGGATT TTTGCTAAGC GAGCCATCCA GACTGGTGAA
    GAGCTGTTTT TCGATTACAG ATACAGCCAG GCCGACGCCC TGAAGTACGT GGGCATCGAA
    AGAGAAATGG AGATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233609
    Clone ID Related Accession (Same CDS sequence) XM_015470758.2
    Accession Version XM_015470758.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGGTCAGA ACCGCTTTCC TGGCTGCCGC TGCAAAGCCC AATGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG CGAGTGCGAC CCTGACCTCT GTCTCACCTG CGGCGCGGCC
    GACCACTGGG ACAGCAAGAA CGTGTCCTGC AAGAACTGCA GCATCCAGCG GGGCTCCAAG
    AAGCATTTAC TGTTGGCACC GTCTGATGTC GCAGGCTGGG GGATCTTCAT CAAGGATCCT
    GTACAGAAAA ATGAATTCAT CTCGGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCA
    GACAGAAGAG GGAAAGTGTA TGACAAGTAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAC
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAGATTC GTTTTGCAAA TCACTCAGTA
    AATCCCAACT GCTATGCAAA AGTGATGATG GTCAACGGCG ATCACAGGAT AGGGATTTTT
    GCTAAGCGAG CCATCCAGAC TGGTGAAGAG CTGTTTTTCG ATTACAGATA CAGCCAGGCC
    GACGCCCTGA AGTACGTGGG CATCGAAAGA GAAATGGAGA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326244.2
    CDS127..2274
    Translation

    Target ORF information:

    RefSeq Version XM_015470758.2
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470758.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAACCCCA GTGCTGAAAT CTCCAGAATA ATCATGGGCC AGACTGGGAA GAAGTCTGAG 
    AAGGGACCAG TGTGTTGGCG GAAGCGCGTA AAGTCAGAGT ACATGCGCCT GCGGCAGCTC
    AAGAGATTCA GGCGCGCTGA CGAAGTCAAG AGTATGTTTA GTTCCAATCG TCAGAAAATT
    TTGGAAAGAA CAGAAATCTT AAACCAAGAG TGGAAACAGC GAAGAATCCA GCCTGTGCAT
    ATCCTGACTT CTGTGAGCTC ATTGCGCGGG ACCAGGGAGT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CGGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGGTCAGA ACCGCTTTCC TGGCTGCCGC TGCAAAGCCC AATGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG CGAGTGCGAC CCTGACCTCT GTCTCACCTG CGGCGCGGCC
    GACCACTGGG ACAGCAAGAA CGTGTCCTGC AAGAACTGCA GCATCCAGCG GGGCTCCAAG
    AAGCATTTAC TGTTGGCACC GTCTGATGTC GCAGGCTGGG GGATCTTCAT CAAGGATCCT
    GTACAGAAAA ATGAATTCAT CTCGGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCA
    GACAGAAGAG GGAAAGTGTA TGACAAGTAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAC
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAGATTC GTTTTGCAAA TCACTCAGTA
    AATCCCAACT GCTATGCAAA AGTGATGATG GTCAACGGCG ATCACAGGAT AGGGATTTTT
    GCTAAGCGAG CCATCCAGAC TGGTGAAGAG CTGTTTTTCG ATTACAGATA CAGCCAGGCC
    GACGCCCTGA AGTACGTGGG CATCGAAAGA GAAATGGAGA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233610
    Clone ID Related Accession (Same CDS sequence) XM_024990586.1 , XM_024990585.1
    Accession Version XM_024990586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGACG GCTCCTCCAA CCACGTTTAC
    AACTATCAAC CCTGTGACCA CCCGCGGCAG CCTTGCGACA GCTCGTGCCC TTGTGTGATA
    GCACAGAATT TTTGTGAAAA ATTTTGTCAG TGTAGTTCAG AGTGTCAGAA CCGCTTTCCT
    GGCTGCCGCT GCAAAGCCCA ATGCAACACC AAGCAGTGCC CGTGCTACCT GGCTGTCCGC
    GAGTGCGACC CTGACCTCTG TCTCACCTGC GGCGCGGCCG ACCACTGGGA CAGCAAGAAC
    GTGTCCTGCA AGAACTGCAG CATCCAGCGG GGCTCCAAGA AGCATTTACT GTTGGCACCG
    TCTGATGTCG CAGGCTGGGG GATCTTCATC AAGGATCCTG TACAGAAAAA TGAATTCATC
    TCGGAATACT GTGGAGAGAT TATTTCTCAA GATGAAGCAG ACAGAAGAGG GAAAGTGTAT
    GACAAGTACA TGTGCAGCTT TCTGTTCAAC TTGAACAACG ATTTTGTGGT GGATGCAACC
    CGCAAGGGTA ACAAGATTCG TTTTGCAAAT CACTCAGTAA ATCCCAACTG CTATGCAAAA
    GTGATGATGG TCAACGGCGA TCACAGGATA GGGATTTTTG CTAAGCGAGC CATCCAGACT
    GGTGAAGAGC TGTTTTTCGA TTACAGATAC AGCCAGGCCG ACGCCCTGAA GTACGTGGGC
    ATCGAAAGAG AAATGGAGAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846354.1
    CDS353..2299
    Translation

    Target ORF information:

    RefSeq Version XM_024990586.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGACG GCTCCTCCAA CCACGTTTAC
    AACTATCAAC CCTGTGACCA CCCGCGGCAG CCTTGCGACA GCTCGTGCCC TTGTGTGATA
    GCACAGAATT TTTGTGAAAA ATTTTGTCAG TGTAGTTCAG AGTGTCAGAA CCGCTTTCCT
    GGCTGCCGCT GCAAAGCCCA ATGCAACACC AAGCAGTGCC CGTGCTACCT GGCTGTCCGC
    GAGTGCGACC CTGACCTCTG TCTCACCTGC GGCGCGGCCG ACCACTGGGA CAGCAAGAAC
    GTGTCCTGCA AGAACTGCAG CATCCAGCGG GGCTCCAAGA AGCATTTACT GTTGGCACCG
    TCTGATGTCG CAGGCTGGGG GATCTTCATC AAGGATCCTG TACAGAAAAA TGAATTCATC
    TCGGAATACT GTGGAGAGAT TATTTCTCAA GATGAAGCAG ACAGAAGAGG GAAAGTGTAT
    GACAAGTACA TGTGCAGCTT TCTGTTCAAC TTGAACAACG ATTTTGTGGT GGATGCAACC
    CGCAAGGGTA ACAAGATTCG TTTTGCAAAT CACTCAGTAA ATCCCAACTG CTATGCAAAA
    GTGATGATGG TCAACGGCGA TCACAGGATA GGGATTTTTG CTAAGCGAGC CATCCAGACT
    GGTGAAGAGC TGTTTTTCGA TTACAGATAC AGCCAGGCCG ACGCCCTGAA GTACGTGGGC
    ATCGAAAGAG AAATGGAGAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233610
    Clone ID Related Accession (Same CDS sequence) XM_024990586.1 , XM_024990585.1
    Accession Version XM_024990585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGACG GCTCCTCCAA CCACGTTTAC
    AACTATCAAC CCTGTGACCA CCCGCGGCAG CCTTGCGACA GCTCGTGCCC TTGTGTGATA
    GCACAGAATT TTTGTGAAAA ATTTTGTCAG TGTAGTTCAG AGTGTCAGAA CCGCTTTCCT
    GGCTGCCGCT GCAAAGCCCA ATGCAACACC AAGCAGTGCC CGTGCTACCT GGCTGTCCGC
    GAGTGCGACC CTGACCTCTG TCTCACCTGC GGCGCGGCCG ACCACTGGGA CAGCAAGAAC
    GTGTCCTGCA AGAACTGCAG CATCCAGCGG GGCTCCAAGA AGCATTTACT GTTGGCACCG
    TCTGATGTCG CAGGCTGGGG GATCTTCATC AAGGATCCTG TACAGAAAAA TGAATTCATC
    TCGGAATACT GTGGAGAGAT TATTTCTCAA GATGAAGCAG ACAGAAGAGG GAAAGTGTAT
    GACAAGTACA TGTGCAGCTT TCTGTTCAAC TTGAACAACG ATTTTGTGGT GGATGCAACC
    CGCAAGGGTA ACAAGATTCG TTTTGCAAAT CACTCAGTAA ATCCCAACTG CTATGCAAAA
    GTGATGATGG TCAACGGCGA TCACAGGATA GGGATTTTTG CTAAGCGAGC CATCCAGACT
    GGTGAAGAGC TGTTTTTCGA TTACAGATAC AGCCAGGCCG ACGCCCTGAA GTACGTGGGC
    ATCGAAAGAG AAATGGAGAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846353.1
    CDS439..2385
    Translation

    Target ORF information:

    RefSeq Version XM_024990585.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGGT
    ACCTCTGTCT TGTTAGATAA AGAAAGCCGT CCACCTCGGA AATTTCCTTC TGATAAAATT
    TTTGAAGCTA TTTCCTCAAT GTTTCCAGAT AAGGGCACAG CAGAAGAGCT AAAGGAAAAG
    TACAAAGAAC TCACGGAGCA GCAGCTCCCA GGTGCGCTTC CTCCTGAGTG CACCCCCAAC
    ATAGATGGAC CAAACGCCAA GTCTGTCCAG AGGGAGCAGA GCTTGCATTC GTTTCATACG
    CTTTTCTGTA GGCGATGTTT TAAATATGAC TGCTTCCTAC ATCGTAAGTG CAGTTATTCT
    TTTCATGCAA CACCCAACAC TTACAAGCGG AAGAACACAG AGACGGCCCT CGACAACAAG
    CCTTGTGGCC CCCACTGTTA CCAGCACTTG GAGGGAGCGA AGGAGTTCGC TGCGGCTCTC
    ACCGCCGAGC GGATCAAGAC CCCCCCGAAG CGCCCGGGGG GCCGCCGGAG AGGCCGGCTT
    CCCAACAACA GCAGCAGGCC CAGCACCCCC ACCATCAACG TGCTGGAGTC GAAGGACACG
    GACAGCGACC GGGAGGCGGG GGCCGAGACT GGCGGGGAGA GCAACGACAA GGACGAGGAG
    GAGAAGAAGG ACGAGACCTC AAGCTCCTCG GAAGCGAATT CTCGGTGTCA AACGCCAATA
    AAGATGAAGC CCAACACGGA GCCCCCCGAG AATGTGGAGT GGAGCGGCGC TGAGGCCTCC
    ATGTTCAGGG TCCTCATCGG CACCTACTAT GACAATTTCT GTGCCATCGC CAGGCTCATC
    GGGACCAAAA CATGTCGACA GGTGTATGAG TTTCGAGTCA AAGAATCCAG TGTCATCGCG
    CCGGCCCCTG CCGAGGACGT GGACACTCCT CCCCGAAAGA AGAAGAGGAA ACACCGGTTG
    TGGGCCGCAC ACTGCAGAAA GATCCAGCTG AAGAAGGACG GCTCCTCCAA CCACGTTTAC
    AACTATCAAC CCTGTGACCA CCCGCGGCAG CCTTGCGACA GCTCGTGCCC TTGTGTGATA
    GCACAGAATT TTTGTGAAAA ATTTTGTCAG TGTAGTTCAG AGTGTCAGAA CCGCTTTCCT
    GGCTGCCGCT GCAAAGCCCA ATGCAACACC AAGCAGTGCC CGTGCTACCT GGCTGTCCGC
    GAGTGCGACC CTGACCTCTG TCTCACCTGC GGCGCGGCCG ACCACTGGGA CAGCAAGAAC
    GTGTCCTGCA AGAACTGCAG CATCCAGCGG GGCTCCAAGA AGCATTTACT GTTGGCACCG
    TCTGATGTCG CAGGCTGGGG GATCTTCATC AAGGATCCTG TACAGAAAAA TGAATTCATC
    TCGGAATACT GTGGAGAGAT TATTTCTCAA GATGAAGCAG ACAGAAGAGG GAAAGTGTAT
    GACAAGTACA TGTGCAGCTT TCTGTTCAAC TTGAACAACG ATTTTGTGGT GGATGCAACC
    CGCAAGGGTA ACAAGATTCG TTTTGCAAAT CACTCAGTAA ATCCCAACTG CTATGCAAAA
    GTGATGATGG TCAACGGCGA TCACAGGATA GGGATTTTTG CTAAGCGAGC CATCCAGACT
    GGTGAAGAGC TGTTTTTCGA TTACAGATAC AGCCAGGCCG ACGCCCTGAA GTACGTGGGC
    ATCGAAAGAG AAATGGAGAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233611
    Clone ID Related Accession (Same CDS sequence) XM_024990587.1
    Accession Version XM_024990587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase EZH2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGACGGCT CCTCCAACCA CGTTTACAAC TATCAACCCT GTGACCACCC GCGGCAGCCT
    TGCGACAGCT CGTGCCCTTG TGTGATAGCA CAGAATTTTT GTGAAAAATT TTGTCAGTGT
    AGTTCAGAGT GTCAGAACCG CTTTCCTGGC TGCCGCTGCA AAGCCCAATG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGCGAG TGCGACCCTG ACCTCTGTCT CACCTGCGGC
    GCGGCCGACC ACTGGGACAG CAAGAACGTG TCCTGCAAGA ACTGCAGCAT CCAGCGGGGC
    TCCAAGAAGC ATTTACTGTT GGCACCGTCT GATGTCGCAG GCTGGGGGAT CTTCATCAAG
    GATCCTGTAC AGAAAAATGA ATTCATCTCG GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCAGACA GAAGAGGGAA AGTGTATGAC AAGTACATGT GCAGCTTTCT GTTCAACTTG
    AACAACGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AGATTCGTTT TGCAAATCAC
    TCAGTAAATC CCAACTGCTA TGCAAAAGTG ATGATGGTCA ACGGCGATCA CAGGATAGGG
    ATTTTTGCTA AGCGAGCCAT CCAGACTGGT GAAGAGCTGT TTTTCGATTA CAGATACAGC
    CAGGCCGACG CCCTGAAGTA CGTGGGCATC GAAAGAGAAA TGGAGATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846355.1
    CDS568..2481
    Translation

    Target ORF information:

    RefSeq Version XM_024990587.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTATTCTT GGTCTCCTCT ACAGCAGAAT TTCATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCAT ACATGGGGGA TGAAGTTTTA GACCAGGATG GGACGTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGAAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGCTGGTGAA TGCTCTTGGT CAGTACAATG ATGATGATGA CGATGATGAC
    GGAGATGATC CTGATGAAAG AGAAGAAAAA CAGAAAGATC TAGAGGAGAG TCGAGAAGAT
    AAAGAAAGCC GTCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC TATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAG CTAAAGGAAA AGTACAAAGA ACTCACGGAG
    CAGCAGCTCC CAGGTGCGCT TCCTCCTGAG TGCACCCCCA ACATAGATGG ACCAAACGCC
    AAGTCTGTCC AGAGGGAGCA GAGCTTGCAT TCGTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA CAAGCGGAAG
    AACACAGAGA CGGCCCTCGA CAACAAGCCT TGTGGCCCCC ACTGTTACCA GCACTTGGAG
    GGAGCGAAGG AGTTCGCTGC GGCTCTCACC GCCGAGCGGA TCAAGACCCC CCCGAAGCGC
    CCGGGGGGCC GCCGGAGAGG CCGGCTTCCC AACAACAGCA GCAGGCCCAG CACCCCCACC
    ATCAACGTGC TGGAGTCGAA GGACACGGAC AGCGACCGGG AGGCGGGGGC CGAGACTGGC
    GGGGAGAGCA ACGACAAGGA CGAGGAGGAG AAGAAGGACG AGACCTCAAG CTCCTCGGAA
    GCGAATTCTC GGTGTCAAAC GCCAATAAAG ATGAAGCCCA ACACGGAGCC CCCCGAGAAT
    GTGGAGTGGA GCGGCGCTGA GGCCTCCATG TTCAGGGTCC TCATCGGCAC CTACTATGAC
    AATTTCTGTG CCATCGCCAG GCTCATCGGG ACCAAAACAT GTCGACAGGT GTATGAGTTT
    CGAGTCAAAG AATCCAGTGT CATCGCGCCG GCCCCTGCCG AGGACGTGGA CACTCCTCCC
    CGAAAGAAGA AGAGGAAACA CCGGTTGTGG GCCGCACACT GCAGAAAGAT CCAGCTGAAG
    AAGGACGGCT CCTCCAACCA CGTTTACAAC TATCAACCCT GTGACCACCC GCGGCAGCCT
    TGCGACAGCT CGTGCCCTTG TGTGATAGCA CAGAATTTTT GTGAAAAATT TTGTCAGTGT
    AGTTCAGAGT GTCAGAACCG CTTTCCTGGC TGCCGCTGCA AAGCCCAATG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGCGAG TGCGACCCTG ACCTCTGTCT CACCTGCGGC
    GCGGCCGACC ACTGGGACAG CAAGAACGTG TCCTGCAAGA ACTGCAGCAT CCAGCGGGGC
    TCCAAGAAGC ATTTACTGTT GGCACCGTCT GATGTCGCAG GCTGGGGGAT CTTCATCAAG
    GATCCTGTAC AGAAAAATGA ATTCATCTCG GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCAGACA GAAGAGGGAA AGTGTATGAC AAGTACATGT GCAGCTTTCT GTTCAACTTG
    AACAACGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AGATTCGTTT TGCAAATCAC
    TCAGTAAATC CCAACTGCTA TGCAAAAGTG ATGATGGTCA ACGGCGATCA CAGGATAGGG
    ATTTTTGCTA AGCGAGCCAT CCAGACTGGT GAAGAGCTGT TTTTCGATTA CAGATACAGC
    CAGGCCGACG CCCTGAAGTA CGTGGGCATC GAAAGAGAAA TGGAGATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.