PDE4DIP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE4DIP
Entrez Gene ID	508547
Full Name	myomegalin
Synonyms	CMYA2,PDE4DIP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015462608.1	XP_015318094.1	myomegalin isoform X6
XM_005197743.3	XP_005197800.1	myomegalin isoform X1
XM_015462611.1	XP_015318097.1	myomegalin isoform X14
XM_005197753.3	XP_005197810.1	myomegalin isoform X19
XM_005204049.3	XP_005204106.1	myomegalin isoform X1
XM_005204052.3	XP_005204109.1	myomegalin isoform X4
XM_010803125.2	XP_010801427.1	myomegalin isoform X8
XM_015463433.1	XP_015318919.1	myomegalin isoform X14
XM_010803129.2	XP_010801431.1	myomegalin isoform X18
XM_024989993.1	XP_024845761.1	myomegalin isoform X11
XM_010803125.3	XP_010801427.1	myomegalin isoform X12
XM_015463430.1	XP_015318916.1	myomegalin isoform X17
XM_005204051.3	XP_005204108.1	myomegalin isoform X2
XM_005204058.3	XP_005204115.1	myomegalin isoform X11
XM_010803126.2	XP_010801428.1	myomegalin isoform X13
XM_005197744.3	XP_005197801.1	myomegalin isoform X3
XM_005197748.3	XP_005197805.1	myomegalin isoform X7
XM_005204052.4	XP_005204109.1	myomegalin isoform X4
XM_024989990.1	XP_024845758.1	myomegalin isoform X5
XM_005204053.2	XP_005204110.1	myomegalin isoform X8
XM_015463432.2	XP_015318918.1	myomegalin isoform X19
XM_005204061.4	XP_005204118.1	myomegalin isoform X23
XM_024989999.1	XP_024845767.1	myomegalin isoform X27
XM_010803130.1	XP_010801432.1	myomegalin isoform X29
XM_010826853.2	XP_010825155.1	myomegalin isoform X11
XM_010826855.2	XP_010825157.1	myomegalin isoform X16
XM_005197745.3	XP_005197802.1	myomegalin isoform X2
XM_005197752.3	XP_005197809.1	myomegalin isoform X17
XM_010803127.2	XP_010801429.1	myomegalin isoform X15
XM_010803128.2	XP_010801430.1	myomegalin isoform X16
XM_005204049.4	XP_005204106.1	myomegalin isoform X1
XM_015463419.1	XP_015318905.1	myomegalin isoform X9
XM_024989994.1	XP_024845762.1	myomegalin isoform X14
XM_010803126.3	XP_010801428.1	myomegalin isoform X20
XM_015463433.2	XP_015318919.1	myomegalin isoform X21
XM_005204062.4	XP_005204119.1	myomegalin isoform X25
XM_015462609.1	XP_015318095.1	myomegalin isoform X10
XM_010826851.1	XP_010825153.1	myomegalin isoform X5
XM_005197749.3	XP_005197806.1	myomegalin isoform X9
XM_015462610.1	XP_015318096.1	myomegalin isoform X12
XM_010826854.2	XP_010825156.1	myomegalin isoform X13
XM_005197750.3	XP_005197807.1	myomegalin isoform X15
XM_010826856.2	XP_010825158.1	myomegalin isoform X18
XM_005204055.3	XP_005204112.1	myomegalin isoform X7
XM_005204056.3	XP_005204113.1	myomegalin isoform X9
XM_015463432.1	XP_015318918.1	myomegalin isoform X12
XM_005204062.3	XP_005204119.1	myomegalin isoform X19
XM_024989995.1	XP_024845763.1	myomegalin isoform X15
XM_005204058.4	XP_005204115.1	myomegalin isoform X18
XM_024989998.1	XP_024845766.1	myomegalin isoform X26
XM_005204050.3	XP_005204107.1	myomegalin isoform X3
XM_005204061.3	XP_005204118.1	myomegalin isoform X17
XM_005197746.3	XP_005197803.1	myomegalin isoform X4
XM_010826852.2	XP_010825154.1	myomegalin isoform X8
XM_010826857.1	XP_010825159.1	myomegalin isoform X20
XM_005204051.4	XP_005204108.1	myomegalin isoform X2
XM_005204050.4	XP_005204107.1	myomegalin isoform X3
XM_024989991.1	XP_024845759.1	myomegalin isoform X6
XM_024989992.1	XP_024845760.1	myomegalin isoform X7
XM_005204055.4	XP_005204112.1	myomegalin isoform X10
XM_005204056.4	XP_005204113.1	myomegalin isoform X13
XM_024989996.1	XP_024845764.1	myomegalin isoform X16
XM_024989997.1	XP_024845765.1	myomegalin isoform X22
XM_010803129.3	XP_010801431.1	myomegalin isoform X24
XM_024990000.1	XP_024845768.1	myomegalin isoform X28

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pde4dip	NP_001034465.2
Gallus gallus (chicken)	PDE4DIP	XP_423459.4
Danio rerio (zebrafish)	si:ch211-242b18.1	XP_005160855.1
Rattus norvegicus (Norway rat)	Pde4dip	NP_071777.1
	PDE4DIP	XP_002801779.1
Bos taurus (cattle)	PDE4DIP	XP_005204110.1
Homo sapiens (human)	PDE4DIP	NP_001185763.3
Canis lupus familiaris (dog)	PDE4DIP	XP_005630746.1

Gene Ontology

The following PDE4DIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE4DIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa03358	XM_005204062.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa03358	XM_005197753.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47202	XM_010803129.3 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa47202	XM_010826856.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa233199	XM_024989990.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233200	XM_024989991.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233201	XM_024989992.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233202	XM_024989993.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233203	XM_024989994.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233204	XM_024989995.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233205	XM_024989996.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233206	XM_024989997.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233207	XM_024989998.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233208	XM_024989999.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa233209	XM_024990000.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa89536	XM_015462610.1 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa89536	XM_015463432.2 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa89537	XM_015463433.2 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa89537	XM_015462611.1 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa03348	XM_005204049.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03348	XM_005197743.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03349	XM_005204050.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03349	XM_005197744.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03350	XM_005204051.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03350	XM_005197745.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03351	XM_005204052.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03351	XM_005197746.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03353	XM_005197748.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03353	XM_005204055.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03354	XM_005204056.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03354	XM_005197749.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03355	XM_010803127.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03355	XM_005197750.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03357	XM_005204061.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03357	XM_005197752.3 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa17544	XM_010826853.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa17544	XM_005204058.4 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47199	XM_010803125.3 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47199	XM_010826852.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47200	XM_010826854.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47200	XM_010803126.3 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47201	XM_010803128.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47201	XM_010826855.2 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa17542	XM_005204053.2 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa17542	XM_010826851.1 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa47203	XM_010826857.1 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa47203	XM_010803130.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa89534	XM_015463419.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa89534	XM_015462608.1 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa89535	XM_015462609.1 Latest version!	Bos taurus myomegalin (LOC508547), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa89535	XM_015463430.1 Latest version!	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
View Old Accession Versions >>
OBa47199	XM_010803125.2	Bos taurus myomegalin (LOC508547), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03351	XM_005204052.3	Bos taurus myomegalin (LOC508547), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03348	XM_005204049.3	Bos taurus myomegalin (LOC508547), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47200	XM_010803126.2	Bos taurus myomegalin (LOC508547), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa89537	XM_015463433.1	Bos taurus myomegalin (LOC508547), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa89536	XM_015463432.1	Bos taurus myomegalin (LOC508547), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa03358	XM_005204062.3	Bos taurus myomegalin (LOC508547), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa17544	XM_005204058.3	Bos taurus myomegalin (LOC508547), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03357	XM_005204061.3	Bos taurus myomegalin (LOC508547), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03350	XM_005204051.3	Bos taurus myomegalin (LOC508547), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03349	XM_005204050.3	Bos taurus myomegalin (LOC508547), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03353	XM_005204055.3	Bos taurus myomegalin (LOC508547), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa03354	XM_005204056.3	Bos taurus myomegalin (LOC508547), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa47202	XM_010803129.2	Bos taurus myomegalin (LOC508547), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE4DIP ( XM_005197753.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197810.1 Bos taurus myomegalin (LOC508547), transcript variant X21, mRNA.

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CloneID	OBa03358
Clone ID Related Accession (Same CDS sequence)	XM_005197753.3 , XM_005204062.4 , XM_005204062.3
Accession Version	XM_005197753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197810.1
CDS	448..3846
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQL

Target ORF information:

RefSeq Version	XM_005197753.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197753.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204062.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204119.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X28, mRNA.

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CloneID	OBa03358
Clone ID Related Accession (Same CDS sequence)	XM_005197753.3 , XM_005204062.4 , XM_005204062.3
Accession Version	XM_005204062.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204062.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204119.1
CDS	718..4116
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQL

Target ORF information:

RefSeq Version	XM_005204062.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204062.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204062.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204119.1 Bos taurus myomegalin (LOC508547), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa03358
Clone ID Related Accession (Same CDS sequence)	XM_005197753.3 , XM_005204062.4 , XM_005204062.3
Accession Version	XM_005204062.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204119.1
CDS	448..3846
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQL

Target ORF information:

RefSeq Version	XM_005204062.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204062.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803129.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801431.1 Bos taurus myomegalin (LOC508547), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa47202
Clone ID Related Accession (Same CDS sequence)	XM_010803129.2 , XM_010826856.2 , XM_010803129.3
Accession Version	XM_010803129.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAATGTCTTC CCTATCCCTT GGCATGTGTT 
CAGTTGCTTC AGTCGTGTCC GACTCTTTGC AACCCCATGG ACTGTAGCCT TCCAGGTTCC 
TCTGTCTATG GGATTCTCCA GGCAAGAATA CTGGAGTGGG TTGCCATTTC CTTCTGCAAT 
CACTTGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801431.1
CDS	452..4003
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQCLPYPLACVQLLQSCPTLCNPMDCSLPGSSVYGILQARILEWVAISFCNHLA

Target ORF information:

RefSeq Version	XM_010803129.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803129.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAATGTCTTC CCTATCCCTT GGCATGTGTT 
CAGTTGCTTC AGTCGTGTCC GACTCTTTGC AACCCCATGG ACTGTAGCCT TCCAGGTTCC 
TCTGTCTATG GGATTCTCCA GGCAAGAATA CTGGAGTGGG TTGCCATTTC CTTCTGCAAT 
CACTTGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826856.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825158.1 Bos taurus myomegalin (LOC508547), transcript variant X20, mRNA.

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CloneID	OBa47202
Clone ID Related Accession (Same CDS sequence)	XM_010803129.2 , XM_010826856.2 , XM_010803129.3
Accession Version	XM_010826856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAATGTCTTC CCTATCCCTT GGCATGTGTT 
CAGTTGCTTC AGTCGTGTCC GACTCTTTGC AACCCCATGG ACTGTAGCCT TCCAGGTTCC 
TCTGTCTATG GGATTCTCCA GGCAAGAATA CTGGAGTGGG TTGCCATTTC CTTCTGCAAT 
CACTTGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825158.1
CDS	452..4003
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQCLPYPLACVQLLQSCPTLCNPMDCSLPGSSVYGILQARILEWVAISFCNHLA

Target ORF information:

RefSeq Version	XM_010826856.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826856.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAATGTCTTC CCTATCCCTT GGCATGTGTT 
CAGTTGCTTC AGTCGTGTCC GACTCTTTGC AACCCCATGG ACTGTAGCCT TCCAGGTTCC 
TCTGTCTATG GGATTCTCCA GGCAAGAATA CTGGAGTGGG TTGCCATTTC CTTCTGCAAT 
CACTTGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803129.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801431.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OBa47202
Clone ID Related Accession (Same CDS sequence)	XM_010803129.2 , XM_010826856.2 , XM_010803129.3
Accession Version	XM_010803129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAATGTCTTC CCTATCCCTT GGCATGTGTT 
CAGTTGCTTC AGTCGTGTCC GACTCTTTGC AACCCCATGG ACTGTAGCCT TCCAGGTTCC 
TCTGTCTATG GGATTCTCCA GGCAAGAATA CTGGAGTGGG TTGCCATTTC CTTCTGCAAT 
CACTTGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801431.1
CDS	719..4270
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQCLPYPLACVQLLQSCPTLCNPMDCSLPGSSVYGILQARILEWVAISFCNHLA

Target ORF information:

RefSeq Version	XM_010803129.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803129.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAATGTCTTC CCTATCCCTT GGCATGTGTT 
CAGTTGCTTC AGTCGTGTCC GACTCTTTGC AACCCCATGG ACTGTAGCCT TCCAGGTTCC 
TCTGTCTATG GGATTCTCCA GGCAAGAATA CTGGAGTGGG TTGCCATTTC CTTCTGCAAT 
CACTTGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989990.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845758.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X5, mRNA.

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CloneID	OBa233199
Clone ID Related Accession (Same CDS sequence)	XM_024989990.1
Accession Version	XM_024989990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845758.1
CDS	714..8396
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989990.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989990.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989991.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845759.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X6, mRNA.

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CloneID	OBa233200
Clone ID Related Accession (Same CDS sequence)	XM_024989991.1
Accession Version	XM_024989991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845759.1
CDS	714..8396
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989991.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989991.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989992.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845760.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X7, mRNA.

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CloneID	OBa233201
Clone ID Related Accession (Same CDS sequence)	XM_024989992.1
Accession Version	XM_024989992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845760.1
CDS	714..8393
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989992.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989992.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989993.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845761.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X11, mRNA.

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CloneID	OBa233202
Clone ID Related Accession (Same CDS sequence)	XM_024989993.1
Accession Version	XM_024989993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GGTGAAATCC CTAAGGGCCC TGCCATGCAC TCCAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845761.1
CDS	714..8276
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_024989993.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989993.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GGTGAAATCC CTAAGGGCCC TGCCATGCAC TCCAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989994.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845762.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X16, mRNA.

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CloneID	OBa233203
Clone ID Related Accession (Same CDS sequence)	XM_024989994.1
Accession Version	XM_024989994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGGTGAA ATCCCTAAGG 
GCCCTGCCAT GCACTCCAGT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845762.1
CDS	714..8240
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_024989994.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989994.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGGTGAA ATCCCTAAGG 
GCCCTGCCAT GCACTCCAGT CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989995.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845763.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X17, mRNA.

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CloneID	OBa233204
Clone ID Related Accession (Same CDS sequence)	XM_024989995.1
Accession Version	XM_024989995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845763.1
CDS	714..8213
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989995.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989995.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989996.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845764.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X19, mRNA.

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CloneID	OBa233205
Clone ID Related Accession (Same CDS sequence)	XM_024989996.1
Accession Version	XM_024989996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGTGT CACCAGTGTT 
CATCCTGCTT CCCCAGCCAC ACTGCCAACC AACCACACAG AAGCCAGATC TTCCCACTAC 
CTCAACCCTC CCCAGCCCCT CTGTCCTCCA AGGGGCACCA CGGACCTGGG CAGAATCCTG 
GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG TGGGACGTGA TGAGGCCTCA GAAAGGGAGC 
ATATCCGGGG ACCTATCCTC AGGCTCCTCC ATGTACCAGC TTAACTCCAA GCCCACAGGG 
GCTGACCTGC TAGAGGAGCA TCTTGGTGAA ATCCGGAACC TACGCCAGCG CCTGGAGGAG 
TCCATCTGCA TTAATGACCG CCTGCGGGAG CAGCTGGAAA ACAGGCTCAG CTCCACTGCC 
CGCGAAAGTG GCTCAACCTC TAACTTCTAC AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC 
TCCAATGAGA ACAGAGTCCT TAGGGAAGAA AACCACAGGC TTCAGGCTCA GCTCAGTCAT 
GTTTCCAGAG AGCACTCCCA GGAAACAGAA TGCCTGAGGG AGGCTCTGCT GACCTCTCGA 
TCCCGGCTTC AAGAGCTGGA AATGGAGCTG GAGCACCAAA AGGTAGACCG GCAGCAGTTA 
CTGGAAGACC TGAAGGAGAA GCAGCAGGAG ATCTTGCATT TCCAGGAGGA ACGACTGTCT 
CTCCAGGAGA AAGACTCCAG GCTGCAGCGT AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA 
GAGAAACAGC AGCTCTTCCA GTCCCTCCAG TCAGAGCTAC AGATCTATGA GGCACTTTAT 
GGTGACTCCA AGAAGACGCT GAAAGCTTAT ACCTGGGATG CCTGTCACCA AGTCCCTTTG 
AGCAGTGACT TGAGCCACCT GGTGGCAGAG ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG 
AGCATTCAGG TGAACAACTG TCTGCGATTG CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC 
AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT AACCAGAAAT TCCCCACCAA CACTGACCCT 
GGAAACAAGC AGCCATTCTT CCAAGATTCG GTTGCCTCCC CTCCAGTTCG GGATGTGGGC 
ATGAATTCTC CAGTTCTCGT CTTCCCCAGC TCTTCTTCTG CGGCTCCTGG CCCAGAGACC 
ACAACCTTCA GTAGGACGAA CGAGCTGGGT TTGGATGCTT CTCCAGTAAT GAAGAACCCT 
CGCAAGCTGG AGGGCGACGC TACTGATGGC TCCTTTGCCA ATAAGCATGG CCGCCACGTC 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGAGAGCAGA TTGGGGAGGG CAGACTGCTG 
GTCAAGAAGA TAGCATCACT GGTGAGACCA ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA 
GGCATAGAGG TGATGGGCAG CGAAGGCATC CAGGAGCTGA GGAGCAGCGC CACCGCCCTG 
CACCACCGGC TGGAGGAGTC GGCCTCCCTC CTCACCATGT TCTGGCGAGC AGCCTTGCCA 
AGCTCCCCCG GCCCTGTGCT TGTGGACAAA GTGGGAGAGT CTATGAAAAA GGAGCTTCTG 
GAACTGAGAA CCAAACTATC CAAACAGGAA AGTCTCCTTC AGAGTACATC TGAGCGTCTG 
AAGACCGCCA ACCAGCAGAA GGAGAGCATG GAGCAGTTCA TCTTCAGCCA GTTGACCAGG 
ACACACGACG TTTTGAAGAA GGCAAGGACT AATTTGGAGA AGACCTCTTA CAAGACTGCT 
TCTGTAAAGC CTTATTCCTG TCCCAGCAAA GGTGAAATCC CTAAGGGCCC TGCCATGCAC 
TCCAGTCCTG TGACCCTTGC CTTCCAGGAG ACATGCAAGA AGCGAAGCCA CCAGAGGTCC 
TTAAAACAGC AGGAAGGATG GGCCTGTGCC CCTTCTTCAC AGCTCCCTGT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845764.1
CDS	714..8210
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989996.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989996.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGTGT CACCAGTGTT 
CATCCTGCTT CCCCAGCCAC ACTGCCAACC AACCACACAG AAGCCAGATC TTCCCACTAC 
CTCAACCCTC CCCAGCCCCT CTGTCCTCCA AGGGGCACCA CGGACCTGGG CAGAATCCTG 
GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG TGGGACGTGA TGAGGCCTCA GAAAGGGAGC 
ATATCCGGGG ACCTATCCTC AGGCTCCTCC ATGTACCAGC TTAACTCCAA GCCCACAGGG 
GCTGACCTGC TAGAGGAGCA TCTTGGTGAA ATCCGGAACC TACGCCAGCG CCTGGAGGAG 
TCCATCTGCA TTAATGACCG CCTGCGGGAG CAGCTGGAAA ACAGGCTCAG CTCCACTGCC 
CGCGAAAGTG GCTCAACCTC TAACTTCTAC AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC 
TCCAATGAGA ACAGAGTCCT TAGGGAAGAA AACCACAGGC TTCAGGCTCA GCTCAGTCAT 
GTTTCCAGAG AGCACTCCCA GGAAACAGAA TGCCTGAGGG AGGCTCTGCT GACCTCTCGA 
TCCCGGCTTC AAGAGCTGGA AATGGAGCTG GAGCACCAAA AGGTAGACCG GCAGCAGTTA 
CTGGAAGACC TGAAGGAGAA GCAGCAGGAG ATCTTGCATT TCCAGGAGGA ACGACTGTCT 
CTCCAGGAGA AAGACTCCAG GCTGCAGCGT AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA 
GAGAAACAGC AGCTCTTCCA GTCCCTCCAG TCAGAGCTAC AGATCTATGA GGCACTTTAT 
GGTGACTCCA AGAAGACGCT GAAAGCTTAT ACCTGGGATG CCTGTCACCA AGTCCCTTTG 
AGCAGTGACT TGAGCCACCT GGTGGCAGAG ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG 
AGCATTCAGG TGAACAACTG TCTGCGATTG CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC 
AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT AACCAGAAAT TCCCCACCAA CACTGACCCT 
GGAAACAAGC AGCCATTCTT CCAAGATTCG GTTGCCTCCC CTCCAGTTCG GGATGTGGGC 
ATGAATTCTC CAGTTCTCGT CTTCCCCAGC TCTTCTTCTG CGGCTCCTGG CCCAGAGACC 
ACAACCTTCA GTAGGACGAA CGAGCTGGGT TTGGATGCTT CTCCAGTAAT GAAGAACCCT 
CGCAAGCTGG AGGGCGACGC TACTGATGGC TCCTTTGCCA ATAAGCATGG CCGCCACGTC 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGAGAGCAGA TTGGGGAGGG CAGACTGCTG 
GTCAAGAAGA TAGCATCACT GGTGAGACCA ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA 
GGCATAGAGG TGATGGGCAG CGAAGGCATC CAGGAGCTGA GGAGCAGCGC CACCGCCCTG 
CACCACCGGC TGGAGGAGTC GGCCTCCCTC CTCACCATGT TCTGGCGAGC AGCCTTGCCA 
AGCTCCCCCG GCCCTGTGCT TGTGGACAAA GTGGGAGAGT CTATGAAAAA GGAGCTTCTG 
GAACTGAGAA CCAAACTATC CAAACAGGAA AGTCTCCTTC AGAGTACATC TGAGCGTCTG 
AAGACCGCCA ACCAGCAGAA GGAGAGCATG GAGCAGTTCA TCTTCAGCCA GTTGACCAGG 
ACACACGACG TTTTGAAGAA GGCAAGGACT AATTTGGAGA AGACCTCTTA CAAGACTGCT 
TCTGTAAAGC CTTATTCCTG TCCCAGCAAA GGTGAAATCC CTAAGGGCCC TGCCATGCAC 
TCCAGTCCTG TGACCCTTGC CTTCCAGGAG ACATGCAAGA AGCGAAGCCA CCAGAGGTCC 
TTAAAACAGC AGGAAGGATG GGCCTGTGCC CCTTCTTCAC AGCTCCCTGT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989997.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845765.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X25, mRNA.

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CloneID	OBa233206
Clone ID Related Accession (Same CDS sequence)	XM_024989997.1
Accession Version	XM_024989997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAG TGGATCGTCG 
GTGAAGCAGG ACAGCAAGCA GGTGCAGGTG GACCTCCAGG ACCTGGGCTA TGAGACCTGT 
GGCCGAAGCG AGAACGAGGC TGAGCGGGAG GAGAGCACCA GCCCTGAGTG TGAGGAGCAC 
GACAGCCTCA GGGAAACAGT CTTGATGGAG GGGCTGTGCT CCAAGCAGGG ACACCTGGAC 
TCCACAATGA CCAGCCCCCC TGGGAAGAAG CCCCTGGAGA GCCAACCAGG GAAGCAGGAG 
GAGCTCCGGG CGTATGGAAA GTCAGAAGAC ATCTCTGTCC TACGTAAGGA CATCAAAGAT 
CTGAAGGCCC AGCTGCAGAA CGCCAACAAG GTCATTCAGA ACCTGAAGAG CCGGGTTCGG 
TCCCTCTCAG TTACAAGCGA TTATTCATCT AGTCTGGAGA GACCCCGAAA GCTGAAGGCC 
GTTGGTGCCC TAGAAGGAAC TTCACCGCAC AGTGTCACTG ATGAGGACGA GGGGTGGCTG 
TCTGATGGCA CTGGGGCTTT CTACCCTCCA GGGCTTCAGG CTAAAAAGGA TCTGGAGAGT 
CTGATCCAGA GGGTGTCCCA GCTAGAAGCC CAGCTCCCAA AAACTGGAAC GGAAGGGAAG 
CTGGCCGAGG AGCTGAGATC TGCTTCGTGG CCTGGGAAAT ATGATTCCCT GATTCAGGAC 
CAGGCCCGAG AACTGTCCTA CCTACGTCAG AAAATACGGG AAGGGAGAGG TATCTGTTAT 
CTTCTCACCC AGCACTCGAA AGACACAGTA AAGTCTTTCG AGGACCTCCT AAGGAGCAAT 
GACATTGACT ACTACCTGGG ACAGAGCTTC CGGGAGCACC TCGCCCAGGG CAGCCAGCTG 
ACAGAAAGGC TCACCAGCAA ACTCAGCACC AAGGATCATA AAAGTGAGAA AGATCAAGCT 
GGACTCGAGC CACTGGCTCT GAGGCTTAGC AGGGAGCTGC AGGAGAAGGA GAAAGTGATC 
GAAGTCCTGC AGGCCAAGCT GGACGCCCGC TCCCTCACAC CGTGCAGCAG CCATGCCTTG 
TCAGACTCTC AGCGCTCTCC CAGCAGCTCC TCCTTCTTGT CTGACGAGCT GGAAGCCTGC 
TCTGACATGG ACATCGCCAG CGAGTACACA CACTATGAAG AGAAGAAGGC TTCACCTGGT 
CACTCAGATT CCATCCATCA TTCGAGTCAT TCTGCTGTAC TGTCTTCCAA ACCATCAGCA 
ACCAGTTCAT CTCAGGAGGT TAAGGCCGAA CCCAGCAGCA ACCCCGTCAG CTTCCCAGCT 
CCCCAGAATC CCCCCAAGGA GGCCAGCCAG GCCCATCCAG GCCTTCATTT CCAATCCATA 
CCCACGCTGG TTAGCCTTCC CCAGGCACCA CTGCCCTCAG CTCCACCCAG CTTCCTGCCT 
TTCAGCTCTG CTGGCCCTCC CCTCCCTGGC TGCTGTGAGA CACCCATGGT CTCCTTGGCA 
GAGGCTCAGC AGGAGCTGCA GATGCTGCGG AAGCAGTTAG GAGAAAGTGT CACCAGTGTT 
CATCCTGCTT CCCCAGCCAC ACTGCCAACC AACCACACAG AAGCCAGATC TTCCCACTAC 
CTCAACCCTC CCCAGCCCCT CTGTCCTCCA AGGGGCACCA CGGACCTGGG CAGAATCCTG 
GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG TGGGACGTGA TGAGGCCTCA GAAAGGGAGC 
ATATCCGGGG ACCTATCCTC AGGCTCCTCC ATGTACCAGC TTAACTCCAA GCCCACAGGG 
GCTGACCTGC TAGAGGAGCA TCTTGGTGAA ATCCGGAACC TACGCCAGCG CCTGGAGGAG 
TCCATCTGCA TTAATGACCG CCTGCGGGAG CAGCTGGAAA ACAGGCTCAG CTCCACTGCC 
CGCGAAAGTG GCTCAACCTC TAACTTCTAC AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC 
TCCAATGAGA ACAGAGTCCT TAGGGAAGAA AACCACAGGC TTCAGGCTCA GCTCAGTCAT 
GTTTCCAGAG AGCACTCCCA GGAAACAGAA TGCCTGAGGG AGGCTCTGCT GACCTCTCGA 
TCCCGGCTTC AAGAGCTGGA AATGGAGCTG GAGCACCAAA AGGTAGACCG GCAGCAGTTA 
CTGGAAGACC TGAAGGAGAA GCAGCAGGAG ATCTTGCATT TCCAGGAGGA ACGACTGTCT 
CTCCAGGAGA AAGACTCCAG GCTGCAGCGT AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA 
GAGAAACAGC AGCTCTTCCA GTCCCTCCAG TCAGAGCTAC AGATCTATGA GGCACTTTAT 
GGTGACTCCA AGAAGACGCT GAAAGCTTAT ACCTGGGATG CCTGTCACCA AGTCCCTTTG 
AGCAGTGACT TGAGCCACCT GGTGGCAGAG ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG 
AGCATTCAGG TGAACAACTG TCTGCGATTG CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC 
AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT AACCAGAAAT TCCCCACCAA CACTGACCCT 
GGAAACAAGC AGCCATTCTT CCAAGATTCG GTTGCCTCCC CTCCAGTTCG GGATGTGGGC 
ATGAATTCTC CAGTTCTCGT CTTCCCCAGC TCTTCTTCTG CGGCTCCTGG CCCAGAGACC 
ACAACCTTCA GTAGGACGAA CGAGCTGGGT TTGGATGCTT CTCCAGTAAT GAAGAACCCT 
CGCAAGCTGG AGGGCGACGC TACTGATGGC TCCTTTGCCA ATAAGCATGG CCGCCACGTC 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGAGAGCAGA TTGGGGAGGG CAGACTGCTG 
GTCAAGAAGA TAGCATCACT GGTGAGACCA ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA 
GGCATAGAGC CACAGGGATG CCCATGGCTA CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA 
GGCATCCAGG AGCTGAGGAG CAGCGCCACC GCCCTGCACC ACCGGCTGGA GGAGTCGGCC 
TCCCTCCTCA CCATGTTCTG GCGAGCAGCC TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG 
GACAAAGTGG GAGAGTCTAT GAAAAAGGAG CTTCTGGAAC TGAGAACCAA ACTATCCAAA 
CAGGAAAGTC TCCTTCAGAG TACATCTGAG CGTCTGAAGA CCGCCAACCA GCAGAAGGAG 
AGCATGGAGC AGTTCATCTT CAGCCAGTTG ACCAGGACAC ACGACGTTTT GAAGAAGGCA 
AGGACTAATT TGGAGAAGAC CTCTTACAAG ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC 
AGCAAAGGTG AAATCCCTAA GGGCCCTGCC ATGCACTCCA GTCCTGTGAC CCTTGCCTTC 
CAGGAGACAT GCAAGAAGCG AAGCCACCAG AGGTCCTTAA AACAGCAGGA AGGATGGGCC 
TGTGCCCCTT CTTCACAGCT CCCTGTCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845765.1
CDS	220..7452
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989997.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989997.1

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAG TGGATCGTCG 
GTGAAGCAGG ACAGCAAGCA GGTGCAGGTG GACCTCCAGG ACCTGGGCTA TGAGACCTGT 
GGCCGAAGCG AGAACGAGGC TGAGCGGGAG GAGAGCACCA GCCCTGAGTG TGAGGAGCAC 
GACAGCCTCA GGGAAACAGT CTTGATGGAG GGGCTGTGCT CCAAGCAGGG ACACCTGGAC 
TCCACAATGA CCAGCCCCCC TGGGAAGAAG CCCCTGGAGA GCCAACCAGG GAAGCAGGAG 
GAGCTCCGGG CGTATGGAAA GTCAGAAGAC ATCTCTGTCC TACGTAAGGA CATCAAAGAT 
CTGAAGGCCC AGCTGCAGAA CGCCAACAAG GTCATTCAGA ACCTGAAGAG CCGGGTTCGG 
TCCCTCTCAG TTACAAGCGA TTATTCATCT AGTCTGGAGA GACCCCGAAA GCTGAAGGCC 
GTTGGTGCCC TAGAAGGAAC TTCACCGCAC AGTGTCACTG ATGAGGACGA GGGGTGGCTG 
TCTGATGGCA CTGGGGCTTT CTACCCTCCA GGGCTTCAGG CTAAAAAGGA TCTGGAGAGT 
CTGATCCAGA GGGTGTCCCA GCTAGAAGCC CAGCTCCCAA AAACTGGAAC GGAAGGGAAG 
CTGGCCGAGG AGCTGAGATC TGCTTCGTGG CCTGGGAAAT ATGATTCCCT GATTCAGGAC 
CAGGCCCGAG AACTGTCCTA CCTACGTCAG AAAATACGGG AAGGGAGAGG TATCTGTTAT 
CTTCTCACCC AGCACTCGAA AGACACAGTA AAGTCTTTCG AGGACCTCCT AAGGAGCAAT 
GACATTGACT ACTACCTGGG ACAGAGCTTC CGGGAGCACC TCGCCCAGGG CAGCCAGCTG 
ACAGAAAGGC TCACCAGCAA ACTCAGCACC AAGGATCATA AAAGTGAGAA AGATCAAGCT 
GGACTCGAGC CACTGGCTCT GAGGCTTAGC AGGGAGCTGC AGGAGAAGGA GAAAGTGATC 
GAAGTCCTGC AGGCCAAGCT GGACGCCCGC TCCCTCACAC CGTGCAGCAG CCATGCCTTG 
TCAGACTCTC AGCGCTCTCC CAGCAGCTCC TCCTTCTTGT CTGACGAGCT GGAAGCCTGC 
TCTGACATGG ACATCGCCAG CGAGTACACA CACTATGAAG AGAAGAAGGC TTCACCTGGT 
CACTCAGATT CCATCCATCA TTCGAGTCAT TCTGCTGTAC TGTCTTCCAA ACCATCAGCA 
ACCAGTTCAT CTCAGGAGGT TAAGGCCGAA CCCAGCAGCA ACCCCGTCAG CTTCCCAGCT 
CCCCAGAATC CCCCCAAGGA GGCCAGCCAG GCCCATCCAG GCCTTCATTT CCAATCCATA 
CCCACGCTGG TTAGCCTTCC CCAGGCACCA CTGCCCTCAG CTCCACCCAG CTTCCTGCCT 
TTCAGCTCTG CTGGCCCTCC CCTCCCTGGC TGCTGTGAGA CACCCATGGT CTCCTTGGCA 
GAGGCTCAGC AGGAGCTGCA GATGCTGCGG AAGCAGTTAG GAGAAAGTGT CACCAGTGTT 
CATCCTGCTT CCCCAGCCAC ACTGCCAACC AACCACACAG AAGCCAGATC TTCCCACTAC 
CTCAACCCTC CCCAGCCCCT CTGTCCTCCA AGGGGCACCA CGGACCTGGG CAGAATCCTG 
GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG TGGGACGTGA TGAGGCCTCA GAAAGGGAGC 
ATATCCGGGG ACCTATCCTC AGGCTCCTCC ATGTACCAGC TTAACTCCAA GCCCACAGGG 
GCTGACCTGC TAGAGGAGCA TCTTGGTGAA ATCCGGAACC TACGCCAGCG CCTGGAGGAG 
TCCATCTGCA TTAATGACCG CCTGCGGGAG CAGCTGGAAA ACAGGCTCAG CTCCACTGCC 
CGCGAAAGTG GCTCAACCTC TAACTTCTAC AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC 
TCCAATGAGA ACAGAGTCCT TAGGGAAGAA AACCACAGGC TTCAGGCTCA GCTCAGTCAT 
GTTTCCAGAG AGCACTCCCA GGAAACAGAA TGCCTGAGGG AGGCTCTGCT GACCTCTCGA 
TCCCGGCTTC AAGAGCTGGA AATGGAGCTG GAGCACCAAA AGGTAGACCG GCAGCAGTTA 
CTGGAAGACC TGAAGGAGAA GCAGCAGGAG ATCTTGCATT TCCAGGAGGA ACGACTGTCT 
CTCCAGGAGA AAGACTCCAG GCTGCAGCGT AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA 
GAGAAACAGC AGCTCTTCCA GTCCCTCCAG TCAGAGCTAC AGATCTATGA GGCACTTTAT 
GGTGACTCCA AGAAGACGCT GAAAGCTTAT ACCTGGGATG CCTGTCACCA AGTCCCTTTG 
AGCAGTGACT TGAGCCACCT GGTGGCAGAG ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG 
AGCATTCAGG TGAACAACTG TCTGCGATTG CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC 
AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT AACCAGAAAT TCCCCACCAA CACTGACCCT 
GGAAACAAGC AGCCATTCTT CCAAGATTCG GTTGCCTCCC CTCCAGTTCG GGATGTGGGC 
ATGAATTCTC CAGTTCTCGT CTTCCCCAGC TCTTCTTCTG CGGCTCCTGG CCCAGAGACC 
ACAACCTTCA GTAGGACGAA CGAGCTGGGT TTGGATGCTT CTCCAGTAAT GAAGAACCCT 
CGCAAGCTGG AGGGCGACGC TACTGATGGC TCCTTTGCCA ATAAGCATGG CCGCCACGTC 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGAGAGCAGA TTGGGGAGGG CAGACTGCTG 
GTCAAGAAGA TAGCATCACT GGTGAGACCA ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA 
GGCATAGAGC CACAGGGATG CCCATGGCTA CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA 
GGCATCCAGG AGCTGAGGAG CAGCGCCACC GCCCTGCACC ACCGGCTGGA GGAGTCGGCC 
TCCCTCCTCA CCATGTTCTG GCGAGCAGCC TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG 
GACAAAGTGG GAGAGTCTAT GAAAAAGGAG CTTCTGGAAC TGAGAACCAA ACTATCCAAA 
CAGGAAAGTC TCCTTCAGAG TACATCTGAG CGTCTGAAGA CCGCCAACCA GCAGAAGGAG 
AGCATGGAGC AGTTCATCTT CAGCCAGTTG ACCAGGACAC ACGACGTTTT GAAGAAGGCA 
AGGACTAATT TGGAGAAGAC CTCTTACAAG ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC 
AGCAAAGGTG AAATCCCTAA GGGCCCTGCC ATGCACTCCA GTCCTGTGAC CCTTGCCTTC 
CAGGAGACAT GCAAGAAGCG AAGCCACCAG AGGTCCTTAA AACAGCAGGA AGGATGGGCC 
TGTGCCCCTT CTTCACAGCT CCCTGTCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989998.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845766.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X29, mRNA.

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CloneID	OBa233207
Clone ID Related Accession (Same CDS sequence)	XM_024989998.1
Accession Version	XM_024989998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGC CTTCATTTCC AATCCATACC CACGCTGGTT 
AGCCTTCCCC AGGCACCACT GCCCTCAGCT CCACCCAGCT TCCTGCCTTT CAGCTCTGCT 
GGCCCTCCCC TCCCTGGCTG CTGTGAGACA CCCATGGTCT CCTTGGCAGA GGCTCAGCAG 
GAGCTGCAGA TGCTGCGGAA GCAGTTAGGA GAAAGTGTCA CCAGTGTTCA TCCTGCTTCC 
CCAGCCACAC TGCCAACCAA CCACACAGAA GCCAGATCTT CCCACTACCT CAACCCTCCC 
CAGCCCCTCT GTCCTCCAAG GGGCACCACG GACCTGGGCA GAATCCTGGA GCCTGGGTAC 
CTGGGTAGCA GTGGCCAGTG GGACGTGATG AGGCCTCAGA AAGGGAGCAT ATCCGGGGAC 
CTATCCTCAG GCTCCTCCAT GTACCAGCTT AACTCCAAGC CCACAGGGGC TGACCTGCTA 
GAGGAGCATC TTGGTGAAAT CCGGAACCTA CGCCAGCGCC TGGAGGAGTC CATCTGCATT 
AATGACCGCC TGCGGGAGCA GCTGGAAAAC AGGCTCAGCT CCACTGCCCG CGAAAGTGGC 
TCAACCTCTA ACTTCTACAG TCCAGGCCTG GAGTCCACAC CTCAGCTCTC CAATGAGAAC 
AGAGTCCTTA GGGAAGAAAA CCACAGGCTT CAGGCTCAGC TCAGTCATGT TTCCAGAGAG 
CACTCCCAGG AAACAGAATG CCTGAGGGAG GCTCTGCTGA CCTCTCGATC CCGGCTTCAA 
GAGCTGGAAA TGGAGCTGGA GCACCAAAAG GTAGACCGGC AGCAGTTACT GGAAGACCTG 
AAGGAGAAGC AGCAGGAGAT CTTGCATTTC CAGGAGGAAC GACTGTCTCT CCAGGAGAAA 
GACTCCAGGC TGCAGCGTAA GCTGGCCCTC CTGCAGCAAC AGTGCGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CACTTTATGG TGACTCCAAG 
AAGACGCTGA AAGCTTATAC CTGGGATGCC TGTCACCAAG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAGAG CATTCAGGTG 
AACAACTGTC TGCGATTGCA GCTCGAACAG CAGTTGGATG GTGGTGCCAG CAAAGCTGGC 
CTCAGCTCCT CCTCCGTTAA CCAGAAATTC CCCACCAACA CTGACCCTGG AAACAAGCAG 
CCATTCTTCC AAGATTCGGT TGCCTCCCCT CCAGTTCGGG ATGTGGGCAT GAATTCTCCA 
GTTCTCGTCT TCCCCAGCTC TTCTTCTGCG GCTCCTGGCC CAGAGACCAC AACCTTCAGT 
AGGACGAACG AGCTGGGTTT GGATGCTTCT CCAGTAATGA AGAACCCTCG CAAGCTGGAG 
GGCGACGCTA CTGATGGCTC CTTTGCCAAT AAGCATGGCC GCCACGTCAT TGGCCACATT 
GATGACTATA GTGCCCTAAG AGAGCAGATT GGGGAGGGCA GACTGCTGGT CAAGAAGATA 
GCATCACTGG TGAGACCAAC CTGCAGCTTC TCTGGTCTTG AAGCTCCAGG CATAGAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845766.1
CDS	16..7620
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024989998.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989998.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGC CTTCATTTCC AATCCATACC CACGCTGGTT 
AGCCTTCCCC AGGCACCACT GCCCTCAGCT CCACCCAGCT TCCTGCCTTT CAGCTCTGCT 
GGCCCTCCCC TCCCTGGCTG CTGTGAGACA CCCATGGTCT CCTTGGCAGA GGCTCAGCAG 
GAGCTGCAGA TGCTGCGGAA GCAGTTAGGA GAAAGTGTCA CCAGTGTTCA TCCTGCTTCC 
CCAGCCACAC TGCCAACCAA CCACACAGAA GCCAGATCTT CCCACTACCT CAACCCTCCC 
CAGCCCCTCT GTCCTCCAAG GGGCACCACG GACCTGGGCA GAATCCTGGA GCCTGGGTAC 
CTGGGTAGCA GTGGCCAGTG GGACGTGATG AGGCCTCAGA AAGGGAGCAT ATCCGGGGAC 
CTATCCTCAG GCTCCTCCAT GTACCAGCTT AACTCCAAGC CCACAGGGGC TGACCTGCTA 
GAGGAGCATC TTGGTGAAAT CCGGAACCTA CGCCAGCGCC TGGAGGAGTC CATCTGCATT 
AATGACCGCC TGCGGGAGCA GCTGGAAAAC AGGCTCAGCT CCACTGCCCG CGAAAGTGGC 
TCAACCTCTA ACTTCTACAG TCCAGGCCTG GAGTCCACAC CTCAGCTCTC CAATGAGAAC 
AGAGTCCTTA GGGAAGAAAA CCACAGGCTT CAGGCTCAGC TCAGTCATGT TTCCAGAGAG 
CACTCCCAGG AAACAGAATG CCTGAGGGAG GCTCTGCTGA CCTCTCGATC CCGGCTTCAA 
GAGCTGGAAA TGGAGCTGGA GCACCAAAAG GTAGACCGGC AGCAGTTACT GGAAGACCTG 
AAGGAGAAGC AGCAGGAGAT CTTGCATTTC CAGGAGGAAC GACTGTCTCT CCAGGAGAAA 
GACTCCAGGC TGCAGCGTAA GCTGGCCCTC CTGCAGCAAC AGTGCGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CACTTTATGG TGACTCCAAG 
AAGACGCTGA AAGCTTATAC CTGGGATGCC TGTCACCAAG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAGAG CATTCAGGTG 
AACAACTGTC TGCGATTGCA GCTCGAACAG CAGTTGGATG GTGGTGCCAG CAAAGCTGGC 
CTCAGCTCCT CCTCCGTTAA CCAGAAATTC CCCACCAACA CTGACCCTGG AAACAAGCAG 
CCATTCTTCC AAGATTCGGT TGCCTCCCCT CCAGTTCGGG ATGTGGGCAT GAATTCTCCA 
GTTCTCGTCT TCCCCAGCTC TTCTTCTGCG GCTCCTGGCC CAGAGACCAC AACCTTCAGT 
AGGACGAACG AGCTGGGTTT GGATGCTTCT CCAGTAATGA AGAACCCTCG CAAGCTGGAG 
GGCGACGCTA CTGATGGCTC CTTTGCCAAT AAGCATGGCC GCCACGTCAT TGGCCACATT 
GATGACTATA GTGCCCTAAG AGAGCAGATT GGGGAGGGCA GACTGCTGGT CAAGAAGATA 
GCATCACTGG TGAGACCAAC CTGCAGCTTC TCTGGTCTTG AAGCTCCAGG CATAGAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024989999.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845767.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X30, mRNA.

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CloneID	OBa233208
Clone ID Related Accession (Same CDS sequence)	XM_024989999.1
Accession Version	XM_024989999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAG TGGATCGTCG 
GTGAAGCAGG ACAGCAAGCA GGTGCAGGTG GACCTCCAGG ACCTGGGCTA TGAGACCTGT 
GGCCGAAGCG AGAACGAGGC TGAGCGGGAG GAGAGCACCA GCCCTGAGTG TGAGGAGCAC 
GACAGCCTCA GGGAAACAGT CTTGATGGAG GGGCTGTGCT CCAAGCAGGG ACACCTGGAC 
TCCACAATGA CCAGCCCCCC TGGGAAGAAG CCCCTGGAGA GCCAACCAGG GAAGCAGGAG 
GAGCTCCGGG CGTATGGAAA GTCAGAAGAC ATCTCTGTCC TACGTAAGGA CATCAAAGAT 
CTGAAGGCCC AGCTGCAGAA CGCCAACAAG GTCATTCAGA ACCTGAAGAG CCGGGTTCGG 
TCCCTCTCAG TTACAAGCGA TTATTCATCT AGTCTGGAGA GACCCCGAAA GCTGAAGGCC 
GTTGGTGCCC TAGAAGGAAC TTCACCGCAC AGTGTCACTG ATGAGGACGA GGGGTGGCTG 
TCTGATGGCA CTGGGGCTTT CTACCCTCCA GGGCTTCAGG CTAAAAAGGA TCTGGAGAGT 
CTGATCCAGA GGGTGTCCCA GCTAGAAGCC CAGCTCCCAA AAACTGGAAC GGAAGGGAAG 
CTGGCCGAGG AGCTGAGATC TGCTTCGTGG CCTGGGAAAT ATGATTCCCT GATTCAGGAC 
CAGGCCCGAG AACTGTCCTA CCTACGTCAG AAAATACGGG AAGGGAGAGG TATCTGTTAT 
CTTCTCACCC AGCACTCGAA AGACACAGTA AAGTCTTTCG AGGACCTCCT AAGGAGCAAT 
GACATTGACT ACTACCTGGG ACAGAGCTTC CGGGAGCACC TCGCCCAGGG CAGCCAGCTG 
ACAGAAAGGC TCACCAGCAA ACTCAGCACC AAGGATCATA AAAGTGAGAA AGATCAAGCT 
GGACTCGAGC CACTGGCTCT GAGGCTTAGC AGGGAGCTGC AGGAGAAGGA GAAAGTGATC 
GAAGTCCTGC AGGCCAAGCT GGACGCCCGC TCCCTCACAC CGTGCAGCAG CCATGCCTTG 
TCAGACTCTC AGCGCTCTCC CAGCAGCTCC TCCTTCTTGT CTGACGAGCT GGAAGCCTGC 
TCTGACATGG ACATCGCCAG CGAGTACACA CACTATGAAG AGAAGAAGGC TTCACCTGGT 
CACTCAGATT CCATCCATCA TTCGAGTCAT TCTGCTGTAC TGTCTTCCAA ACCATCAGCA 
ACCAGTTCAT CTCAGGAGGT TAAGGCCGAA CCCAGCAGCA ACCCCGTCAG CTTCCCAGCT 
CCCCAGAATC CCCCCAAGGA GGCCAGCCAG GCCCATCCAG GCCTTCATTT CCAATCCATA 
CCCACGCTGG TTAGCCTTCC CCAGGCACCA CTGCCCTCAG CTCCACCCAG CTTCCTGCCT 
TTCAGCTCTG CTGGCCCTCC CCTCCCTGGC TGCTGTGAGA CACCCATGGT CTCCTTGGCA 
GAGGCTCAGC AGGAGCTGCA GATGCTGCGG AAGCAGTTAG GAGAAAGTGT CACCAGTGTT 
CATCCTGCTT CCCCAGCCAC ACTGCCAACC AACCACACAG AAGCCAGATC TTCCCACTAC 
CTCAACCCTC CCCAGCCCCT CTGTCCTCCA AGGGGCACCA CGGACCTGGG CAGAATCCTG 
GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG TGGGACGTGA TGAGGCCTCA GAAAGGGAGC 
ATATCCGGGG ACCTATCCTC AGGCTCCTCC ATGTACCAGC TTAACTCCAA GCCCACAGGG 
GCTGACCTGC TAGAGGAGCA TCTTGGTGAA ATCCGGAACC TACGCCAGCG CCTGGAGGAG 
TCCATCTGCA TTAATGACCG CCTGCGGGAG CAGCTGGAAA ACAGGCTCAG CTCCACTGCC 
CGCGAAAGTG GCTCAACCTC TAACTTCTAC AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC 
TCCAATGAGA ACAGAGTCCT TAGGGAAGAA AACCACAGGC TTCAGGCTCA GCTCAGTCAT 
GTTTCCAGAG AGCACTCCCA GGAAACAGAA TGCCTGAGGG AGGCTCTGCT GACCTCTCGA 
TCCCGGCTTC AAGAGCTGGA AATGGAGCTG GAGCACCAAA AGGTAGACCG GCAGCAGTTA 
CTGGAAGACC TGAAGGAGAA GCAGCAGGAG ATCTTGCATT TCCAGGAGGA ACGACTGTCT 
CTCCAGGAGA AAGACTCCAG GCTGCAGCGT AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA 
GAGAAACAGC AGCTCTTCCA GTCCCTCCAG TCAGAGCTAC AGATCTATGA GGCACTTTAT 
GGTGACTCCA AGAAGACGCT GAAAGCTTAT ACCTGGGATG CCTGTCACCA AGTCCCTTTG 
AGCAGTGACT TGAGCCACCT GGTGGCAGAG ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG 
AGCATTCAGG TGAACAACTG TCTGCGATTG CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC 
AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT AACCAGAAAT TCCCCACCAA CACTGACCCT 
GGAAACAAGC AGCCATTCTT CCAAGATTCG GTTGCCTCCC CTCCAGTTCG GGATGTGGGC 
ATGAATTCTC CAGTTCTCGT CTTCCCCAGC TCTTCTTCTG CGGCTCCTGG CCCAGAGACC 
ACAACCTTCA GTAGGACGAA CGAGCTGGGT TTGGATGCTT CTCCAGTAAT GAAGAACCCT 
CGCAAGCTGG AGGGCGACGC TACTGATGGC TCCTTTGCCA ATAAGCATGG CCGCCACGTC 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGAGAGCAGA TTGGGGAGGG CAGACTGCTG 
GTCAAGAAGA TAGCATCACT GGTGAGACCA ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA 
GGCATAGAGG TGATGGGCAG CGAAGGCATC CAGGAGCTGA GGAGCAGCGC CACCGCCCTG 
CACCACCGGC TGGAGGAGTC GGCCTCCCTC CTCACCATGT TCTGGCGAGC AGCCTTGCCA 
AGCTCCCCCG GCCCTGTGCT TGTGGACAAA GTGGGAGAGT CTATGAAAAA GGAGCTTCTG 
GAACTGAGAA CCAAACTATC CAAACAGGAA AGTCTCCTTC AGAGTACATC TGAGCGTCTG 
AAGACCGCCA ACCAGCAGAA GGAGAGCATG GAGCAGTTCA TCTTCAGCCA GTTGACCAGG 
ACACACGACG TTTTGAAGAA GGCAAGGACT AATTTGGAGG TGAAATCCCT AAGGGCCCTG 
CCATGCACTC CAGTCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845767.1
CDS	220..7260
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_024989999.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989999.1

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAG TGGATCGTCG 
GTGAAGCAGG ACAGCAAGCA GGTGCAGGTG GACCTCCAGG ACCTGGGCTA TGAGACCTGT 
GGCCGAAGCG AGAACGAGGC TGAGCGGGAG GAGAGCACCA GCCCTGAGTG TGAGGAGCAC 
GACAGCCTCA GGGAAACAGT CTTGATGGAG GGGCTGTGCT CCAAGCAGGG ACACCTGGAC 
TCCACAATGA CCAGCCCCCC TGGGAAGAAG CCCCTGGAGA GCCAACCAGG GAAGCAGGAG 
GAGCTCCGGG CGTATGGAAA GTCAGAAGAC ATCTCTGTCC TACGTAAGGA CATCAAAGAT 
CTGAAGGCCC AGCTGCAGAA CGCCAACAAG GTCATTCAGA ACCTGAAGAG CCGGGTTCGG 
TCCCTCTCAG TTACAAGCGA TTATTCATCT AGTCTGGAGA GACCCCGAAA GCTGAAGGCC 
GTTGGTGCCC TAGAAGGAAC TTCACCGCAC AGTGTCACTG ATGAGGACGA GGGGTGGCTG 
TCTGATGGCA CTGGGGCTTT CTACCCTCCA GGGCTTCAGG CTAAAAAGGA TCTGGAGAGT 
CTGATCCAGA GGGTGTCCCA GCTAGAAGCC CAGCTCCCAA AAACTGGAAC GGAAGGGAAG 
CTGGCCGAGG AGCTGAGATC TGCTTCGTGG CCTGGGAAAT ATGATTCCCT GATTCAGGAC 
CAGGCCCGAG AACTGTCCTA CCTACGTCAG AAAATACGGG AAGGGAGAGG TATCTGTTAT 
CTTCTCACCC AGCACTCGAA AGACACAGTA AAGTCTTTCG AGGACCTCCT AAGGAGCAAT 
GACATTGACT ACTACCTGGG ACAGAGCTTC CGGGAGCACC TCGCCCAGGG CAGCCAGCTG 
ACAGAAAGGC TCACCAGCAA ACTCAGCACC AAGGATCATA AAAGTGAGAA AGATCAAGCT 
GGACTCGAGC CACTGGCTCT GAGGCTTAGC AGGGAGCTGC AGGAGAAGGA GAAAGTGATC 
GAAGTCCTGC AGGCCAAGCT GGACGCCCGC TCCCTCACAC CGTGCAGCAG CCATGCCTTG 
TCAGACTCTC AGCGCTCTCC CAGCAGCTCC TCCTTCTTGT CTGACGAGCT GGAAGCCTGC 
TCTGACATGG ACATCGCCAG CGAGTACACA CACTATGAAG AGAAGAAGGC TTCACCTGGT 
CACTCAGATT CCATCCATCA TTCGAGTCAT TCTGCTGTAC TGTCTTCCAA ACCATCAGCA 
ACCAGTTCAT CTCAGGAGGT TAAGGCCGAA CCCAGCAGCA ACCCCGTCAG CTTCCCAGCT 
CCCCAGAATC CCCCCAAGGA GGCCAGCCAG GCCCATCCAG GCCTTCATTT CCAATCCATA 
CCCACGCTGG TTAGCCTTCC CCAGGCACCA CTGCCCTCAG CTCCACCCAG CTTCCTGCCT 
TTCAGCTCTG CTGGCCCTCC CCTCCCTGGC TGCTGTGAGA CACCCATGGT CTCCTTGGCA 
GAGGCTCAGC AGGAGCTGCA GATGCTGCGG AAGCAGTTAG GAGAAAGTGT CACCAGTGTT 
CATCCTGCTT CCCCAGCCAC ACTGCCAACC AACCACACAG AAGCCAGATC TTCCCACTAC 
CTCAACCCTC CCCAGCCCCT CTGTCCTCCA AGGGGCACCA CGGACCTGGG CAGAATCCTG 
GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG TGGGACGTGA TGAGGCCTCA GAAAGGGAGC 
ATATCCGGGG ACCTATCCTC AGGCTCCTCC ATGTACCAGC TTAACTCCAA GCCCACAGGG 
GCTGACCTGC TAGAGGAGCA TCTTGGTGAA ATCCGGAACC TACGCCAGCG CCTGGAGGAG 
TCCATCTGCA TTAATGACCG CCTGCGGGAG CAGCTGGAAA ACAGGCTCAG CTCCACTGCC 
CGCGAAAGTG GCTCAACCTC TAACTTCTAC AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC 
TCCAATGAGA ACAGAGTCCT TAGGGAAGAA AACCACAGGC TTCAGGCTCA GCTCAGTCAT 
GTTTCCAGAG AGCACTCCCA GGAAACAGAA TGCCTGAGGG AGGCTCTGCT GACCTCTCGA 
TCCCGGCTTC AAGAGCTGGA AATGGAGCTG GAGCACCAAA AGGTAGACCG GCAGCAGTTA 
CTGGAAGACC TGAAGGAGAA GCAGCAGGAG ATCTTGCATT TCCAGGAGGA ACGACTGTCT 
CTCCAGGAGA AAGACTCCAG GCTGCAGCGT AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA 
GAGAAACAGC AGCTCTTCCA GTCCCTCCAG TCAGAGCTAC AGATCTATGA GGCACTTTAT 
GGTGACTCCA AGAAGACGCT GAAAGCTTAT ACCTGGGATG CCTGTCACCA AGTCCCTTTG 
AGCAGTGACT TGAGCCACCT GGTGGCAGAG ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG 
AGCATTCAGG TGAACAACTG TCTGCGATTG CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC 
AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT AACCAGAAAT TCCCCACCAA CACTGACCCT 
GGAAACAAGC AGCCATTCTT CCAAGATTCG GTTGCCTCCC CTCCAGTTCG GGATGTGGGC 
ATGAATTCTC CAGTTCTCGT CTTCCCCAGC TCTTCTTCTG CGGCTCCTGG CCCAGAGACC 
ACAACCTTCA GTAGGACGAA CGAGCTGGGT TTGGATGCTT CTCCAGTAAT GAAGAACCCT 
CGCAAGCTGG AGGGCGACGC TACTGATGGC TCCTTTGCCA ATAAGCATGG CCGCCACGTC 
ATTGGCCACA TTGATGACTA TAGTGCCCTA AGAGAGCAGA TTGGGGAGGG CAGACTGCTG 
GTCAAGAAGA TAGCATCACT GGTGAGACCA ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA 
GGCATAGAGG TGATGGGCAG CGAAGGCATC CAGGAGCTGA GGAGCAGCGC CACCGCCCTG 
CACCACCGGC TGGAGGAGTC GGCCTCCCTC CTCACCATGT TCTGGCGAGC AGCCTTGCCA 
AGCTCCCCCG GCCCTGTGCT TGTGGACAAA GTGGGAGAGT CTATGAAAAA GGAGCTTCTG 
GAACTGAGAA CCAAACTATC CAAACAGGAA AGTCTCCTTC AGAGTACATC TGAGCGTCTG 
AAGACCGCCA ACCAGCAGAA GGAGAGCATG GAGCAGTTCA TCTTCAGCCA GTTGACCAGG 
ACACACGACG TTTTGAAGAA GGCAAGGACT AATTTGGAGG TGAAATCCCT AAGGGCCCTG 
CCATGCACTC CAGTCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_024990000.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845768.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X31, mRNA.

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CloneID	OBa233209
Clone ID Related Accession (Same CDS sequence)	XM_024990000.1
Accession Version	XM_024990000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGTGTC ACCAGTGTTC ATCCTGCTTC CCCAGCCACA 
CTGCCAACCA ACCACACAGA AGCCAGATCT TCCCACTACC TCAACCCTCC CCAGCCCCTC 
TGTCCTCCAA GGGGCACCAC GGACCTGGGC AGAATCCTGG AGCCTGGGTA CCTGGGTAGC 
AGTGGCCAGT GGGACGTGAT GAGGCCTCAG AAAGGGAGCA TATCCGGGGA CCTATCCTCA 
GGCTCCTCCA TGTACCAGCT TAACTCCAAG CCCACAGGGG CTGACCTGCT AGAGGAGCAT 
CTTGGTGAAA TCCGGAACCT ACGCCAGCGC CTGGAGGAGT CCATCTGCAT TAATGACCGC 
CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC TCCACTGCCC GCGAAAGTGG CTCAACCTCT 
AACTTCTACA GTCCAGGCCT GGAGTCCACA CCTCAGCTCT CCAATGAGAA CAGAGTCCTT 
AGGGAAGAAA ACCACAGGCT TCAGGCTCAG CTCAGTCATG TTTCCAGAGA GCACTCCCAG 
GAAACAGAAT GCCTGAGGGA GGCTCTGCTG ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA 
ATGGAGCTGG AGCACCAAAA GGTAGACCGG CAGCAGTTAC TGGAAGACCT GAAGGAGAAG 
CAGCAGGAGA TCTTGCATTT CCAGGAGGAA CGACTGTCTC TCCAGGAGAA AGACTCCAGG 
CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA CAGTGCGAAG AGAAACAGCA GCTCTTCCAG 
TCCCTCCAGT CAGAGCTACA GATCTATGAG GCACTTTATG GTGACTCCAA GAAGACGCTG 
AAAGCTTATA CCTGGGATGC CTGTCACCAA GTCCCTTTGA GCAGTGACTT GAGCCACCTG 
GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG CTGGAGCAGA GCATTCAGGT GAACAACTGT 
CTGCGATTGC AGCTCGAACA GCAGTTGGAT GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC 
TCCTCCGTTA ACCAGAAATT CCCCACCAAC ACTGACCCTG GAAACAAGCA GCCATTCTTC 
CAAGATTCGG TTGCCTCCCC TCCAGTTCGG GATGTGGGCA TGAATTCTCC AGTTCTCGTC 
TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC CCAGAGACCA CAACCTTCAG TAGGACGAAC 
GAGCTGGGTT TGGATGCTTC TCCAGTAATG AAGAACCCTC GCAAGCTGGA GGGCGACGCT 
ACTGATGGCT CCTTTGCCAA TAAGCATGGC CGCCACGTCA TTGGCCACAT TGATGACTAT 
AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC AGACTGCTGG TCAAGAAGAT AGCATCACTG 
GTGAGACCAA CCTGCAGCTT CTCTGGTCTT GAAGCTCCAG GCATAGAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845768.1
CDS	16..7431
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_024990000.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990000.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGTGTC ACCAGTGTTC ATCCTGCTTC CCCAGCCACA 
CTGCCAACCA ACCACACAGA AGCCAGATCT TCCCACTACC TCAACCCTCC CCAGCCCCTC 
TGTCCTCCAA GGGGCACCAC GGACCTGGGC AGAATCCTGG AGCCTGGGTA CCTGGGTAGC 
AGTGGCCAGT GGGACGTGAT GAGGCCTCAG AAAGGGAGCA TATCCGGGGA CCTATCCTCA 
GGCTCCTCCA TGTACCAGCT TAACTCCAAG CCCACAGGGG CTGACCTGCT AGAGGAGCAT 
CTTGGTGAAA TCCGGAACCT ACGCCAGCGC CTGGAGGAGT CCATCTGCAT TAATGACCGC 
CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC TCCACTGCCC GCGAAAGTGG CTCAACCTCT 
AACTTCTACA GTCCAGGCCT GGAGTCCACA CCTCAGCTCT CCAATGAGAA CAGAGTCCTT 
AGGGAAGAAA ACCACAGGCT TCAGGCTCAG CTCAGTCATG TTTCCAGAGA GCACTCCCAG 
GAAACAGAAT GCCTGAGGGA GGCTCTGCTG ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA 
ATGGAGCTGG AGCACCAAAA GGTAGACCGG CAGCAGTTAC TGGAAGACCT GAAGGAGAAG 
CAGCAGGAGA TCTTGCATTT CCAGGAGGAA CGACTGTCTC TCCAGGAGAA AGACTCCAGG 
CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA CAGTGCGAAG AGAAACAGCA GCTCTTCCAG 
TCCCTCCAGT CAGAGCTACA GATCTATGAG GCACTTTATG GTGACTCCAA GAAGACGCTG 
AAAGCTTATA CCTGGGATGC CTGTCACCAA GTCCCTTTGA GCAGTGACTT GAGCCACCTG 
GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG CTGGAGCAGA GCATTCAGGT GAACAACTGT 
CTGCGATTGC AGCTCGAACA GCAGTTGGAT GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC 
TCCTCCGTTA ACCAGAAATT CCCCACCAAC ACTGACCCTG GAAACAAGCA GCCATTCTTC 
CAAGATTCGG TTGCCTCCCC TCCAGTTCGG GATGTGGGCA TGAATTCTCC AGTTCTCGTC 
TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC CCAGAGACCA CAACCTTCAG TAGGACGAAC 
GAGCTGGGTT TGGATGCTTC TCCAGTAATG AAGAACCCTC GCAAGCTGGA GGGCGACGCT 
ACTGATGGCT CCTTTGCCAA TAAGCATGGC CGCCACGTCA TTGGCCACAT TGATGACTAT 
AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC AGACTGCTGG TCAAGAAGAT AGCATCACTG 
GTGAGACCAA CCTGCAGCTT CTCTGGTCTT GAAGCTCCAG GCATAGAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015463432.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318918.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X22, mRNA.

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CloneID	OBa89536
Clone ID Related Accession (Same CDS sequence)	XM_015463432.2 , XM_015462610.1 , XM_015463432.1
Accession Version	XM_015463432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015463432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGGTGT CACCAGTGTT CATCCTGCTT CCCCAGCCAC ACTGCCAACC 
AACCACACAG AAGCCAGATC TTCCCACTAC CTCAACCCTC CCCAGCCCCT CTGTCCTCCA 
AGGGGCACCA CGGACCTGGG CAGAATCCTG GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG 
TGGGACGTGA TGAGGCCTCA GAAAGGGAGC ATATCCGGGG ACCTATCCTC AGGCTCCTCC 
ATGTACCAGC TTAACTCCAA GCCCACAGGG GCTGACCTGC TAGAGGAGCA TCTTGGTGAA 
ATCCGGAACC TACGCCAGCG CCTGGAGGAG TCCATCTGCA TTAATGACCG CCTGCGGGAG 
CAGCTGGAAA ACAGGCTCAG CTCCACTGCC CGCGAAAGTG GCTCAACCTC TAACTTCTAC 
AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC TCCAATGAGA ACAGAGTCCT TAGGGAAGAA 
AACCACAGGC TTCAGGCTCA GCTCAGTCAT GTTTCCAGAG AGCACTCCCA GGAAACAGAA 
TGCCTGAGGG AGGCTCTGCT GACCTCTCGA TCCCGGCTTC AAGAGCTGGA AATGGAGCTG 
GAGCACCAAA AGGTAGACCG GCAGCAGTTA CTGGAAGACC TGAAGGAGAA GCAGCAGGAG 
ATCTTGCATT TCCAGGAGGA ACGACTGTCT CTCCAGGAGA AAGACTCCAG GCTGCAGCGT 
AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA GAGAAACAGC AGCTCTTCCA GTCCCTCCAG 
TCAGAGCTAC AGATCTATGA GGCACTTTAT GGTGACTCCA AGAAGACGCT GAAAGCTTAT 
ACCTGGGATG CCTGTCACCA AGTCCCTTTG AGCAGTGACT TGAGCCACCT GGTGGCAGAG 
ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG AGCATTCAGG TGAACAACTG TCTGCGATTG 
CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT 
AACCAGAAAT TCCCCACCAA CACTGACCCT GGAAACAAGC AGCCATTCTT CCAAGATTCG 
GTTGCCTCCC CTCCAGTTCG GGATGTGGGC ATGAATTCTC CAGTTCTCGT CTTCCCCAGC 
TCTTCTTCTG CGGCTCCTGG CCCAGAGACC ACAACCTTCA GTAGGACGAA CGAGCTGGGT 
TTGGATGCTT CTCCAGTAAT GAAGAACCCT CGCAAGCTGG AGGGCGACGC TACTGATGGC 
TCCTTTGCCA ATAAGCATGG CCGCCACGTC ATTGGCCACA TTGATGACTA TAGTGCCCTA 
AGAGAGCAGA TTGGGGAGGG CAGACTGCTG GTCAAGAAGA TAGCATCACT GGTGAGACCA 
ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA GGCATAGAGC CACAGGGATG CCCATGGCTA 
CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318918.1
CDS	714..8096
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015463432.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463432.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGGTGT CACCAGTGTT CATCCTGCTT CCCCAGCCAC ACTGCCAACC 
AACCACACAG AAGCCAGATC TTCCCACTAC CTCAACCCTC CCCAGCCCCT CTGTCCTCCA 
AGGGGCACCA CGGACCTGGG CAGAATCCTG GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG 
TGGGACGTGA TGAGGCCTCA GAAAGGGAGC ATATCCGGGG ACCTATCCTC AGGCTCCTCC 
ATGTACCAGC TTAACTCCAA GCCCACAGGG GCTGACCTGC TAGAGGAGCA TCTTGGTGAA 
ATCCGGAACC TACGCCAGCG CCTGGAGGAG TCCATCTGCA TTAATGACCG CCTGCGGGAG 
CAGCTGGAAA ACAGGCTCAG CTCCACTGCC CGCGAAAGTG GCTCAACCTC TAACTTCTAC 
AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC TCCAATGAGA ACAGAGTCCT TAGGGAAGAA 
AACCACAGGC TTCAGGCTCA GCTCAGTCAT GTTTCCAGAG AGCACTCCCA GGAAACAGAA 
TGCCTGAGGG AGGCTCTGCT GACCTCTCGA TCCCGGCTTC AAGAGCTGGA AATGGAGCTG 
GAGCACCAAA AGGTAGACCG GCAGCAGTTA CTGGAAGACC TGAAGGAGAA GCAGCAGGAG 
ATCTTGCATT TCCAGGAGGA ACGACTGTCT CTCCAGGAGA AAGACTCCAG GCTGCAGCGT 
AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA GAGAAACAGC AGCTCTTCCA GTCCCTCCAG 
TCAGAGCTAC AGATCTATGA GGCACTTTAT GGTGACTCCA AGAAGACGCT GAAAGCTTAT 
ACCTGGGATG CCTGTCACCA AGTCCCTTTG AGCAGTGACT TGAGCCACCT GGTGGCAGAG 
ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG AGCATTCAGG TGAACAACTG TCTGCGATTG 
CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT 
AACCAGAAAT TCCCCACCAA CACTGACCCT GGAAACAAGC AGCCATTCTT CCAAGATTCG 
GTTGCCTCCC CTCCAGTTCG GGATGTGGGC ATGAATTCTC CAGTTCTCGT CTTCCCCAGC 
TCTTCTTCTG CGGCTCCTGG CCCAGAGACC ACAACCTTCA GTAGGACGAA CGAGCTGGGT 
TTGGATGCTT CTCCAGTAAT GAAGAACCCT CGCAAGCTGG AGGGCGACGC TACTGATGGC 
TCCTTTGCCA ATAAGCATGG CCGCCACGTC ATTGGCCACA TTGATGACTA TAGTGCCCTA 
AGAGAGCAGA TTGGGGAGGG CAGACTGCTG GTCAAGAAGA TAGCATCACT GGTGAGACCA 
ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA GGCATAGAGC CACAGGGATG CCCATGGCTA 
CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015462610.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318096.1 Bos taurus myomegalin (LOC508547), transcript variant X14, mRNA.

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CloneID	OBa89536
Clone ID Related Accession (Same CDS sequence)	XM_015463432.2 , XM_015462610.1 , XM_015463432.1
Accession Version	XM_015462610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGGTGT CACCAGTGTT CATCCTGCTT CCCCAGCCAC ACTGCCAACC 
AACCACACAG AAGCCAGATC TTCCCACTAC CTCAACCCTC CCCAGCCCCT CTGTCCTCCA 
AGGGGCACCA CGGACCTGGG CAGAATCCTG GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG 
TGGGACGTGA TGAGGCCTCA GAAAGGGAGC ATATCCGGGG ACCTATCCTC AGGCTCCTCC 
ATGTACCAGC TTAACTCCAA GCCCACAGGG GCTGACCTGC TAGAGGAGCA TCTTGGTGAA 
ATCCGGAACC TACGCCAGCG CCTGGAGGAG TCCATCTGCA TTAATGACCG CCTGCGGGAG 
CAGCTGGAAA ACAGGCTCAG CTCCACTGCC CGCGAAAGTG GCTCAACCTC TAACTTCTAC 
AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC TCCAATGAGA ACAGAGTCCT TAGGGAAGAA 
AACCACAGGC TTCAGGCTCA GCTCAGTCAT GTTTCCAGAG AGCACTCCCA GGAAACAGAA 
TGCCTGAGGG AGGCTCTGCT GACCTCTCGA TCCCGGCTTC AAGAGCTGGA AATGGAGCTG 
GAGCACCAAA AGGTAGACCG GCAGCAGTTA CTGGAAGACC TGAAGGAGAA GCAGCAGGAG 
ATCTTGCATT TCCAGGAGGA ACGACTGTCT CTCCAGGAGA AAGACTCCAG GCTGCAGCGT 
AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA GAGAAACAGC AGCTCTTCCA GTCCCTCCAG 
TCAGAGCTAC AGATCTATGA GGCACTTTAT GGTGACTCCA AGAAGACGCT GAAAGCTTAT 
ACCTGGGATG CCTGTCACCA AGTCCCTTTG AGCAGTGACT TGAGCCACCT GGTGGCAGAG 
ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG AGCATTCAGG TGAACAACTG TCTGCGATTG 
CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT 
AACCAGAAAT TCCCCACCAA CACTGACCCT GGAAACAAGC AGCCATTCTT CCAAGATTCG 
GTTGCCTCCC CTCCAGTTCG GGATGTGGGC ATGAATTCTC CAGTTCTCGT CTTCCCCAGC 
TCTTCTTCTG CGGCTCCTGG CCCAGAGACC ACAACCTTCA GTAGGACGAA CGAGCTGGGT 
TTGGATGCTT CTCCAGTAAT GAAGAACCCT CGCAAGCTGG AGGGCGACGC TACTGATGGC 
TCCTTTGCCA ATAAGCATGG CCGCCACGTC ATTGGCCACA TTGATGACTA TAGTGCCCTA 
AGAGAGCAGA TTGGGGAGGG CAGACTGCTG GTCAAGAAGA TAGCATCACT GGTGAGACCA 
ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA GGCATAGAGC CACAGGGATG CCCATGGCTA 
CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318096.1
CDS	433..7815
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015462610.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462610.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGGTGT CACCAGTGTT CATCCTGCTT CCCCAGCCAC ACTGCCAACC 
AACCACACAG AAGCCAGATC TTCCCACTAC CTCAACCCTC CCCAGCCCCT CTGTCCTCCA 
AGGGGCACCA CGGACCTGGG CAGAATCCTG GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG 
TGGGACGTGA TGAGGCCTCA GAAAGGGAGC ATATCCGGGG ACCTATCCTC AGGCTCCTCC 
ATGTACCAGC TTAACTCCAA GCCCACAGGG GCTGACCTGC TAGAGGAGCA TCTTGGTGAA 
ATCCGGAACC TACGCCAGCG CCTGGAGGAG TCCATCTGCA TTAATGACCG CCTGCGGGAG 
CAGCTGGAAA ACAGGCTCAG CTCCACTGCC CGCGAAAGTG GCTCAACCTC TAACTTCTAC 
AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC TCCAATGAGA ACAGAGTCCT TAGGGAAGAA 
AACCACAGGC TTCAGGCTCA GCTCAGTCAT GTTTCCAGAG AGCACTCCCA GGAAACAGAA 
TGCCTGAGGG AGGCTCTGCT GACCTCTCGA TCCCGGCTTC AAGAGCTGGA AATGGAGCTG 
GAGCACCAAA AGGTAGACCG GCAGCAGTTA CTGGAAGACC TGAAGGAGAA GCAGCAGGAG 
ATCTTGCATT TCCAGGAGGA ACGACTGTCT CTCCAGGAGA AAGACTCCAG GCTGCAGCGT 
AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA GAGAAACAGC AGCTCTTCCA GTCCCTCCAG 
TCAGAGCTAC AGATCTATGA GGCACTTTAT GGTGACTCCA AGAAGACGCT GAAAGCTTAT 
ACCTGGGATG CCTGTCACCA AGTCCCTTTG AGCAGTGACT TGAGCCACCT GGTGGCAGAG 
ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG AGCATTCAGG TGAACAACTG TCTGCGATTG 
CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT 
AACCAGAAAT TCCCCACCAA CACTGACCCT GGAAACAAGC AGCCATTCTT CCAAGATTCG 
GTTGCCTCCC CTCCAGTTCG GGATGTGGGC ATGAATTCTC CAGTTCTCGT CTTCCCCAGC 
TCTTCTTCTG CGGCTCCTGG CCCAGAGACC ACAACCTTCA GTAGGACGAA CGAGCTGGGT 
TTGGATGCTT CTCCAGTAAT GAAGAACCCT CGCAAGCTGG AGGGCGACGC TACTGATGGC 
TCCTTTGCCA ATAAGCATGG CCGCCACGTC ATTGGCCACA TTGATGACTA TAGTGCCCTA 
AGAGAGCAGA TTGGGGAGGG CAGACTGCTG GTCAAGAAGA TAGCATCACT GGTGAGACCA 
ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA GGCATAGAGC CACAGGGATG CCCATGGCTA 
CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015463432.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318918.1 Bos taurus myomegalin (LOC508547), transcript variant X14, mRNA.

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CloneID	OBa89536
Clone ID Related Accession (Same CDS sequence)	XM_015463432.2 , XM_015462610.1 , XM_015463432.1
Accession Version	XM_015463432.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGGTGT CACCAGTGTT CATCCTGCTT CCCCAGCCAC ACTGCCAACC 
AACCACACAG AAGCCAGATC TTCCCACTAC CTCAACCCTC CCCAGCCCCT CTGTCCTCCA 
AGGGGCACCA CGGACCTGGG CAGAATCCTG GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG 
TGGGACGTGA TGAGGCCTCA GAAAGGGAGC ATATCCGGGG ACCTATCCTC AGGCTCCTCC 
ATGTACCAGC TTAACTCCAA GCCCACAGGG GCTGACCTGC TAGAGGAGCA TCTTGGTGAA 
ATCCGGAACC TACGCCAGCG CCTGGAGGAG TCCATCTGCA TTAATGACCG CCTGCGGGAG 
CAGCTGGAAA ACAGGCTCAG CTCCACTGCC CGCGAAAGTG GCTCAACCTC TAACTTCTAC 
AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC TCCAATGAGA ACAGAGTCCT TAGGGAAGAA 
AACCACAGGC TTCAGGCTCA GCTCAGTCAT GTTTCCAGAG AGCACTCCCA GGAAACAGAA 
TGCCTGAGGG AGGCTCTGCT GACCTCTCGA TCCCGGCTTC AAGAGCTGGA AATGGAGCTG 
GAGCACCAAA AGGTAGACCG GCAGCAGTTA CTGGAAGACC TGAAGGAGAA GCAGCAGGAG 
ATCTTGCATT TCCAGGAGGA ACGACTGTCT CTCCAGGAGA AAGACTCCAG GCTGCAGCGT 
AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA GAGAAACAGC AGCTCTTCCA GTCCCTCCAG 
TCAGAGCTAC AGATCTATGA GGCACTTTAT GGTGACTCCA AGAAGACGCT GAAAGCTTAT 
ACCTGGGATG CCTGTCACCA AGTCCCTTTG AGCAGTGACT TGAGCCACCT GGTGGCAGAG 
ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG AGCATTCAGG TGAACAACTG TCTGCGATTG 
CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT 
AACCAGAAAT TCCCCACCAA CACTGACCCT GGAAACAAGC AGCCATTCTT CCAAGATTCG 
GTTGCCTCCC CTCCAGTTCG GGATGTGGGC ATGAATTCTC CAGTTCTCGT CTTCCCCAGC 
TCTTCTTCTG CGGCTCCTGG CCCAGAGACC ACAACCTTCA GTAGGACGAA CGAGCTGGGT 
TTGGATGCTT CTCCAGTAAT GAAGAACCCT CGCAAGCTGG AGGGCGACGC TACTGATGGC 
TCCTTTGCCA ATAAGCATGG CCGCCACGTC ATTGGCCACA TTGATGACTA TAGTGCCCTA 
AGAGAGCAGA TTGGGGAGGG CAGACTGCTG GTCAAGAAGA TAGCATCACT GGTGAGACCA 
ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA GGCATAGAGC CACAGGGATG CCCATGGCTA 
CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318918.1
CDS	433..7815
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015463432.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463432.1

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGGTGT CACCAGTGTT CATCCTGCTT CCCCAGCCAC ACTGCCAACC 
AACCACACAG AAGCCAGATC TTCCCACTAC CTCAACCCTC CCCAGCCCCT CTGTCCTCCA 
AGGGGCACCA CGGACCTGGG CAGAATCCTG GAGCCTGGGT ACCTGGGTAG CAGTGGCCAG 
TGGGACGTGA TGAGGCCTCA GAAAGGGAGC ATATCCGGGG ACCTATCCTC AGGCTCCTCC 
ATGTACCAGC TTAACTCCAA GCCCACAGGG GCTGACCTGC TAGAGGAGCA TCTTGGTGAA 
ATCCGGAACC TACGCCAGCG CCTGGAGGAG TCCATCTGCA TTAATGACCG CCTGCGGGAG 
CAGCTGGAAA ACAGGCTCAG CTCCACTGCC CGCGAAAGTG GCTCAACCTC TAACTTCTAC 
AGTCCAGGCC TGGAGTCCAC ACCTCAGCTC TCCAATGAGA ACAGAGTCCT TAGGGAAGAA 
AACCACAGGC TTCAGGCTCA GCTCAGTCAT GTTTCCAGAG AGCACTCCCA GGAAACAGAA 
TGCCTGAGGG AGGCTCTGCT GACCTCTCGA TCCCGGCTTC AAGAGCTGGA AATGGAGCTG 
GAGCACCAAA AGGTAGACCG GCAGCAGTTA CTGGAAGACC TGAAGGAGAA GCAGCAGGAG 
ATCTTGCATT TCCAGGAGGA ACGACTGTCT CTCCAGGAGA AAGACTCCAG GCTGCAGCGT 
AAGCTGGCCC TCCTGCAGCA ACAGTGCGAA GAGAAACAGC AGCTCTTCCA GTCCCTCCAG 
TCAGAGCTAC AGATCTATGA GGCACTTTAT GGTGACTCCA AGAAGACGCT GAAAGCTTAT 
ACCTGGGATG CCTGTCACCA AGTCCCTTTG AGCAGTGACT TGAGCCACCT GGTGGCAGAG 
ATCCGAGCTT TGCGAGGGCA GCTGGAGCAG AGCATTCAGG TGAACAACTG TCTGCGATTG 
CAGCTCGAAC AGCAGTTGGA TGGTGGTGCC AGCAAAGCTG GCCTCAGCTC CTCCTCCGTT 
AACCAGAAAT TCCCCACCAA CACTGACCCT GGAAACAAGC AGCCATTCTT CCAAGATTCG 
GTTGCCTCCC CTCCAGTTCG GGATGTGGGC ATGAATTCTC CAGTTCTCGT CTTCCCCAGC 
TCTTCTTCTG CGGCTCCTGG CCCAGAGACC ACAACCTTCA GTAGGACGAA CGAGCTGGGT 
TTGGATGCTT CTCCAGTAAT GAAGAACCCT CGCAAGCTGG AGGGCGACGC TACTGATGGC 
TCCTTTGCCA ATAAGCATGG CCGCCACGTC ATTGGCCACA TTGATGACTA TAGTGCCCTA 
AGAGAGCAGA TTGGGGAGGG CAGACTGCTG GTCAAGAAGA TAGCATCACT GGTGAGACCA 
ACCTGCAGCT TCTCTGGTCT TGAAGCTCCA GGCATAGAGC CACAGGGATG CCCATGGCTA 
CTGCCCCTTT CCCAGGTGAT GGGCAGCGAA GGCATCCAGG AGCTGAGGAG CAGCGCCACC 
GCCCTGCACC ACCGGCTGGA GGAGTCGGCC TCCCTCCTCA CCATGTTCTG GCGAGCAGCC 
TTGCCAAGCT CCCCCGGCCC TGTGCTTGTG GACAAAGTGG GAGAGTCTAT GAAAAAGGAG 
CTTCTGGAAC TGAGAACCAA ACTATCCAAA CAGGAAAGTC TCCTTCAGAG TACATCTGAG 
CGTCTGAAGA CCGCCAACCA GCAGAAGGAG AGCATGGAGC AGTTCATCTT CAGCCAGTTG 
ACCAGGACAC ACGACGTTTT GAAGAAGGCA AGGACTAATT TGGAGAAGAC CTCTTACAAG 
ACTGCTTCTG TAAAGCCTTA TTCCTGTCCC AGCAAAGGTG AAATCCCTAA GGGCCCTGCC 
ATGCACTCCA GTCCTGTGAC CCTTGCCTTC CAGGAGACAT GCAAGAAGCG AAGCCACCAG 
AGGTCCTTAA AACAGCAGGA AGGATGGGCC TGTGCCCCTT CTTCACAGCT CCCTGTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015462611.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318097.1 Bos taurus myomegalin (LOC508547), transcript variant X16, mRNA.

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CloneID	OBa89537
Clone ID Related Accession (Same CDS sequence)	XM_015462611.1 , XM_015463433.1 , XM_015463433.2
Accession Version	XM_015462611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC ACCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAC TTCCTGGAGG AGCGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGATGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGAG AACTCCAGGA CAAGAGACAG CATCCGGATA AAACATGCGC TGATGCAGAG 
AATATCAACA GCCATAATGA AGCTGAGCTC CGGCGCCAGT TTGAGGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAAGA GGAGTCCAGA 
CTAGCAAAAA ATGAAGCCGC ACGGATGGCG GCTCTGGTGG AAGCAGAGAA AGAGTGTAAC 
CTGGAGCTCT CAGAGAAACT GAAGGGAGTC ACCAAGGAGA GGGAAGATGT ACCAGGAGAC 
CGGGTCAAAT CTGACCAGTA CACTGAGGCC CTGGCCCAGA GGGACAAAAA AATTGAAGAA 
CTGAGTCAAA GCCTGGCTGC ACAGGAGAAA CTTGTAGAGC AGCTGTCTCA AGAGAAACAA 
CAACTGCTAC ATCTGTTGGA GGAGCCAGCT GACATGGAAG TGCAGCCCAT GACTGAAGAC 
TTACTCAAAC AACAGAAGTT GAATTCAAAT GAGACCGGTA CAACTCAGCA GTCTGTATCT 
GATTCCCATC TGGCAGAACT CCAGGAAAAA ATCCAGCAAA CAGAGACCAC CAACAAGATG 
CTTCAAGAAA AACTGAATGA AATGAGCTAT GAACTGAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG ATACAATTCA AAACCTCAAG GAAACTCTGA AAAGCAGGGA AAGTGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AACGACACAA TGGCTAAGCT TCGAGAAATG 
CTGCACCAGA GTCAGCTTAA ACAGCTTCAT AACTCAGAGG GTACTTCCCC AGCTCAGCAA 
CAGGTGGCTC TGCTTGATCT TCAAAGTGCT TTATTCTGCA GCCAGCTTGA AATACAGAAG 
CTCCAGAGGG CAGTACGCCA AAAAGAAAGG CAGCTGGCTG ATGCTAGACG ATGTGTGCGG 
TTTGTAGAGG CTGCAGCACA GGAGAGAGAA CAGCAGAAAG AGGCTTCTTG GAAACATAAC 
CAAGAATTGC AAAAAGCCCT ACAGCAGCTA CAAGGAGAAC TTCAGAGTAA GAGCCAACAG 
CTTCATACCC TGGAGGCTGA AAAATATAAT GAGATTCGCA CCCAAGAGCA GCACATTCAG 
CACCTCCACT ACAGTCTGAG TCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTACCGAA ATAACTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
AGGCAAAGAA TACAAGATAG AGATGTTGCC CTAGAGCGGG CTATAGATGA AAAGTTCTCC 
AGCCTAGAAG AAAAGGAAAA AGAACTGCGT CAGCTTCACC TTGCCATGAG AGAGCGAGAT 
CACGACTTAG AGAGGCTGCG CGGTATCCTC TCCGCCAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGTCCAA AGGCCTGGAA GTGGAACAGT TGTCTGCCAC CTGTCAGAAC 
CTCCAGTGGC TGAAAGAAGA AATGGAAACC AAATTTAGCC GTTGGCAGAA GGAGCAAGAA 
AGCATCATTC AGCAGCTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTT 
ACCGCAACAT TGCTCTGCAA ACTTGGACCA GGGCAGACTG AGATAGTTGA GGAGCTGTGC 
CAGCGTCTAC AGCAGAAGGA AAGGATGCTG CAGGACCTTC TGAGTGATCG GAACAAGCAA 
GCAGTGGAAT ATGAAATGGA GATTCAGGGC CTGCTTCAGT CCATGAGCAC CAGAGAGCAG 
GAGAGCCACG CTGCTGCAGA AAAGATGGTA CAAGCTCTAA TGGAAAGAAA TTCAGAATTA 
CAGGCCCTGC GCCAATATTT AGGAGGGAAA GATTTCATGA TGTCCCAAGC ACCCCTCTCT 
AACCGACCAG CTGAAGTTAC CTCCATCAGC CCCCATCTTG GAGAGCAAAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAG AGATGATAGC ACTTCATTGA CTGCCAAAGG GGATGCTAGC 
GTACCCAGGT CCTCGTTAGG GGAGGTGGAT ACAGTTGCGG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAACTTGA GCTCATGGCT AAAAAAGAGA GAGAAAGTCG GATGGAACTG 
TCTGCACTCC AGTCCATGGT GGCTGTGCAG GAGCAAGAGC TGCAGGTTCA GGCTGCTGAC 
ATGGAGTCCC TGACCAGGAC CATACAGATT AAGGAAGACC TCATAAAGGA CCTGCAGATG 
CAGCTGGTTG ATCCTGAAGA CATCCCAGCT ATGGAACGCC TGACCCAGGA AGTCTTGCTT 
CTTCGGGAAA AAGTGGCTTC AGTAGAATCA CAAGGACAGG AAACGTCTGG AAACCGAAGA 
CAACAATTGC TGCTGATGCT CGAAGGACTG GTAGATGAGC GGAGTCGGCT CAATGAGGCC 
TTACAGGCAG AGAGACAGCT CTATAGCAGT CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGCAATCGG 
CTAGAAGAAG TTCTCGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCCGCC 
ATTGGAGGTA CAGCTGCAGG GGACGACACG GAGGATGCGA GCACGGAGTT TACCGACAGT 
ATCGAGGAGG AGGCTGCACA CAACAGCCAC CAGCAACTCA TCAAGGTGGC GTTGGAGAAA 
AGCCGGGCAG CTGTGGAGAC CCAGAATGTG TCTCCTGCTC CTCCCACCCT GACAGGAGGG 
GACGGTAACA GGGGTCTTCA GGAGGAAATG CTCCACCTGA GGGCTGAAAT TCACCGGCAC 
TTAGAGGAGA AGAGGAAAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT TGAGGAAGAC 
AAGGAGAAGG GCGAGGCGAT GGTGGAGACT GTGGTTGCCA AAGGGGTTCT GAATGAGAAT 
AGCCTTCAGG CTGAATTCAG AAAGGTCCAG GGAAAACTGA AGAATGCCCG CAATATCATC 
AGCCTCCTCA AAGAACAGCT GGTGTTGAGC AGCAAGGAAG GGACTAGTAA GCTTAATCCT 
GAGCTCCTTG TGCACCTGGC CAAGGAGATC AACAGAATGA ACACAGAGCT GGTTTACTCT 
CCTGGAAAGC ACCAATGCCT AGAAGAGGAG GGTGTGACCA CAAGGCCGTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TGCCCTCACA GTGGATGCTC ACCAACTGGA TAACCAGCCC 
CAGACCCATG ACCCTGGGCC GCAGTCAGAA TTTAGCTTCT CAGGATCCAC GAAGCACCTG 
CGCTCCCAGT TGGCACAATG CAAACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCAGAAGCCA CTGTCTTTGC CCAGGCCAAC CAGCTGGAGA AATACAGAGT CATGTTTACA 
GGTGGATCGT CGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGACCTGGGC 
TATGAGACCT GTGGCCGAAG CGAGAACGAG GCTGAGCGGG AGGAGAGCAC CAGCCCTGAG 
TGTGAGGAGC ACGACAGCCT CAGGGAAACA GTCTTGATGG AGGGGCTGTG CTCCAAGCAG 
GGACACCTGG ACTCCACAAT GACCAGCCCC CCTGGGAAGA AGCCCCTGGA GAGCCAACCA 
GGGAAGCAGG AGGAGCTCCG GGCGTATGGA AAGTCAGAAG ACATCTCTGT CCTACGTAAG 
GACATCAAAG ATCTGAAGGC CCAGCTGCAG AACGCCAACA AGGTCATTCA GAACCTGAAG 
AGCCGGGTTC GGTCCCTCTC AGTTACAAGC GATTATTCAT CTAGTCTGGA GAGACCCCGA 
AAGCTGAAGG CCGTTGGTGC CCTAGAAGGA ACTTCACCGC ACAGTGTCAC TGATGAGGAC 
GAGGGGTGGC TGTCTGATGG CACTGGGGCT TTCTACCCTC CAGGGCTTCA GGCTAAAAAG 
GATCTGGAGA GTCTGATCCA GAGGGTGTCC CAGCTAGAAG CCCAGCTCCC AAAAACTGGA 
ACGGAAGGGA AGCTGGCCGA GGAGCTGAGA TCTGCTTCGT GGCCTGGGAA ATATGATTCC 
CTGATTCAGG ACCAGGCCCG AGAACTGTCC TACCTACGTC AGAAAATACG GGAAGGGAGA 
GGTATCTGTT ATCTTCTCAC CCAGCACTCG AAAGACACAG TAAAGTCTTT CGAGGACCTC 
CTAAGGAGCA ATGACATTGA CTACTACCTG GGACAGAGCT TCCGGGAGCA CCTCGCCCAG 
GGCAGCCAGC TGACAGAAAG GCTCACCAGC AAACTCAGCA CCAAGGATCA TAAAAGTGAG 
AAAGATCAAG CTGGACTCGA GCCACTGGCT CTGAGGCTTA GCAGGGAGCT GCAGGAGAAG 
GAGAAAGTGA TCGAAGTCCT GCAGGCCAAG CTGGACGCCC GCTCCCTCAC ACCGTGCAGC 
AGCCATGCCT TGTCAGACTC TCAGCGCTCT CCCAGCAGCT CCTCCTTCTT GTCTGACGAG 
CTGGAAGCCT GCTCTGACAT GGACATCGCC AGCGAGTACA CACACTATGA AGAGAAGAAG 
GCTTCACCTG GTCACTCAGA TTCCATCCAT CATTCGAGTC ATTCTGCTGT ACTGTCTTCC 
AAACCATCAG CAACCAGTTC ATCTCAGGAG GTTAAGGCCG AACCCAGCAG CAACCCCGTC 
AGCTTCCCAG CTCCCCAGAA TCCCCCCAAG GAGGCCAGCC AGGCCCATCC AGGCCTTCAT 
TTCCAATCCA TACCCACGCT GGTTAGCCTT CCCCAGGCAC CACTGCCCTC AGCTCCACCC 
AGCTTCCTGC CTTTCAGCTC TGCTGGCCCT CCCCTCCCTG GCTGCTGTGA GACACCCATG 
GTCTCCTTGG CAGAGGCTCA GCAGGAGCTG CAGATGCTGC GGAAGCAGTT AGGAGAAAGT 
GTCACCAGTG TTCATCCTGC TTCCCCAGCC ACACTGCCAA CCAACCACAC AGAAGCCAGA 
TCTTCCCACT ACCTCAACCC TCCCCAGCCC CTCTGTCCTC CAAGGGGCAC CACGGACCTG 
GGCAGAATCC TGGAGCCTGG GTACCTGGGT AGCAGTGGCC AGTGGGACGT GATGAGGCCT 
CAGAAAGGGA GCATATCCGG GGACCTATCC TCAGGCTCCT CCATGTACCA GCTTAACTCC 
AAGCCCACAG GGGCTGACCT GCTAGAGGAG CATCTTGGTG AAATCCGGAA CCTACGCCAG 
CGCCTGGAGG AGTCCATCTG CATTAATGAC CGCCTGCGGG AGCAGCTGGA AAACAGGCTC 
AGCTCCACTG CCCGCGAAAG TGGCTCAACC TCTAACTTCT ACAGTCCAGG CCTGGAGTCC 
ACACCTCAGC TCTCCAATGA GAACAGAGTC CTTAGGGAAG AAAACCACAG GCTTCAGGCT 
CAGCTCAGTC ATGTTTCCAG AGAGCACTCC CAGGAAACAG AATGCCTGAG GGAGGCTCTG 
CTGACCTCTC GATCCCGGCT TCAAGAGCTG GAAATGGAGC TGGAGCACCA AAAGGTAGAC 
CGGCAGCAGT TACTGGAAGA CCTGAAGGAG AAGCAGCAGG AGATCTTGCA TTTCCAGGAG 
GAACGACTGT CTCTCCAGGA GAAAGACTCC AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG 
CAACAGTGCG AAGAGAAACA GCAGCTCTTC CAGTCCCTCC AGTCAGAGCT ACAGATCTAT 
GAGGCACTTT ATGGTGACTC CAAGAAGACG CTGAAAGCTT ATACCTGGGA TGCCTGTCAC 
CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC CTGGTGGCAG AGATCCGAGC TTTGCGAGGG 
CAGCTGGAGC AGAGCATTCA GGTGAACAAC TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG 
GATGGTGGTG CCAGCAAAGC TGGCCTCAGC TCCTCCTCCG TTAACCAGAA ATTCCCCACC 
AACACTGACC CTGGAAACAA GCAGCCATTC TTCCAAGATT CGGTTGCCTC CCCTCCAGTT 
CGGGATGTGG GCATGAATTC TCCAGTTCTC GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT 
GGCCCAGAGA CCACAACCTT CAGTAGGACG AACGAGCTGG GTTTGGATGC TTCTCCAGTA 
ATGAAGAACC CTCGCAAGCT GGAGGGCGAC GCTACTGATG GCTCCTTTGC CAATAAGCAT 
GGCCGCCACG TCATTGGCCA CATTGATGAC TATAGTGCCC TAAGAGAGCA GATTGGGGAG 
GGCAGACTGC TGGTCAAGAA GATAGCATCA CTGGTGAGAC CAACCTGCAG CTTCTCTGGT 
CTTGAAGCTC CAGGCATAGA GCCACAGGGA TGCCCATGGC TACTGCCCCT TTCCCAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318097.1
CDS	323..7567
Translation	MMGRWYPEQVMQQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015462611.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462611.1

ORF Insert Sequence:

ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC ACCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAC TTCCTGGAGG AGCGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGATGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGAG AACTCCAGGA CAAGAGACAG CATCCGGATA AAACATGCGC TGATGCAGAG 
AATATCAACA GCCATAATGA AGCTGAGCTC CGGCGCCAGT TTGAGGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAAGA GGAGTCCAGA 
CTAGCAAAAA ATGAAGCCGC ACGGATGGCG GCTCTGGTGG AAGCAGAGAA AGAGTGTAAC 
CTGGAGCTCT CAGAGAAACT GAAGGGAGTC ACCAAGGAGA GGGAAGATGT ACCAGGAGAC 
CGGGTCAAAT CTGACCAGTA CACTGAGGCC CTGGCCCAGA GGGACAAAAA AATTGAAGAA 
CTGAGTCAAA GCCTGGCTGC ACAGGAGAAA CTTGTAGAGC AGCTGTCTCA AGAGAAACAA 
CAACTGCTAC ATCTGTTGGA GGAGCCAGCT GACATGGAAG TGCAGCCCAT GACTGAAGAC 
TTACTCAAAC AACAGAAGTT GAATTCAAAT GAGACCGGTA CAACTCAGCA GTCTGTATCT 
GATTCCCATC TGGCAGAACT CCAGGAAAAA ATCCAGCAAA CAGAGACCAC CAACAAGATG 
CTTCAAGAAA AACTGAATGA AATGAGCTAT GAACTGAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG ATACAATTCA AAACCTCAAG GAAACTCTGA AAAGCAGGGA AAGTGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AACGACACAA TGGCTAAGCT TCGAGAAATG 
CTGCACCAGA GTCAGCTTAA ACAGCTTCAT AACTCAGAGG GTACTTCCCC AGCTCAGCAA 
CAGGTGGCTC TGCTTGATCT TCAAAGTGCT TTATTCTGCA GCCAGCTTGA AATACAGAAG 
CTCCAGAGGG CAGTACGCCA AAAAGAAAGG CAGCTGGCTG ATGCTAGACG ATGTGTGCGG 
TTTGTAGAGG CTGCAGCACA GGAGAGAGAA CAGCAGAAAG AGGCTTCTTG GAAACATAAC 
CAAGAATTGC AAAAAGCCCT ACAGCAGCTA CAAGGAGAAC TTCAGAGTAA GAGCCAACAG 
CTTCATACCC TGGAGGCTGA AAAATATAAT GAGATTCGCA CCCAAGAGCA GCACATTCAG 
CACCTCCACT ACAGTCTGAG TCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTACCGAA ATAACTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
AGGCAAAGAA TACAAGATAG AGATGTTGCC CTAGAGCGGG CTATAGATGA AAAGTTCTCC 
AGCCTAGAAG AAAAGGAAAA AGAACTGCGT CAGCTTCACC TTGCCATGAG AGAGCGAGAT 
CACGACTTAG AGAGGCTGCG CGGTATCCTC TCCGCCAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGTCCAA AGGCCTGGAA GTGGAACAGT TGTCTGCCAC CTGTCAGAAC 
CTCCAGTGGC TGAAAGAAGA AATGGAAACC AAATTTAGCC GTTGGCAGAA GGAGCAAGAA 
AGCATCATTC AGCAGCTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTT 
ACCGCAACAT TGCTCTGCAA ACTTGGACCA GGGCAGACTG AGATAGTTGA GGAGCTGTGC 
CAGCGTCTAC AGCAGAAGGA AAGGATGCTG CAGGACCTTC TGAGTGATCG GAACAAGCAA 
GCAGTGGAAT ATGAAATGGA GATTCAGGGC CTGCTTCAGT CCATGAGCAC CAGAGAGCAG 
GAGAGCCACG CTGCTGCAGA AAAGATGGTA CAAGCTCTAA TGGAAAGAAA TTCAGAATTA 
CAGGCCCTGC GCCAATATTT AGGAGGGAAA GATTTCATGA TGTCCCAAGC ACCCCTCTCT 
AACCGACCAG CTGAAGTTAC CTCCATCAGC CCCCATCTTG GAGAGCAAAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAG AGATGATAGC ACTTCATTGA CTGCCAAAGG GGATGCTAGC 
GTACCCAGGT CCTCGTTAGG GGAGGTGGAT ACAGTTGCGG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAACTTGA GCTCATGGCT AAAAAAGAGA GAGAAAGTCG GATGGAACTG 
TCTGCACTCC AGTCCATGGT GGCTGTGCAG GAGCAAGAGC TGCAGGTTCA GGCTGCTGAC 
ATGGAGTCCC TGACCAGGAC CATACAGATT AAGGAAGACC TCATAAAGGA CCTGCAGATG 
CAGCTGGTTG ATCCTGAAGA CATCCCAGCT ATGGAACGCC TGACCCAGGA AGTCTTGCTT 
CTTCGGGAAA AAGTGGCTTC AGTAGAATCA CAAGGACAGG AAACGTCTGG AAACCGAAGA 
CAACAATTGC TGCTGATGCT CGAAGGACTG GTAGATGAGC GGAGTCGGCT CAATGAGGCC 
TTACAGGCAG AGAGACAGCT CTATAGCAGT CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGCAATCGG 
CTAGAAGAAG TTCTCGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCCGCC 
ATTGGAGGTA CAGCTGCAGG GGACGACACG GAGGATGCGA GCACGGAGTT TACCGACAGT 
ATCGAGGAGG AGGCTGCACA CAACAGCCAC CAGCAACTCA TCAAGGTGGC GTTGGAGAAA 
AGCCGGGCAG CTGTGGAGAC CCAGAATGTG TCTCCTGCTC CTCCCACCCT GACAGGAGGG 
GACGGTAACA GGGGTCTTCA GGAGGAAATG CTCCACCTGA GGGCTGAAAT TCACCGGCAC 
TTAGAGGAGA AGAGGAAAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT TGAGGAAGAC 
AAGGAGAAGG GCGAGGCGAT GGTGGAGACT GTGGTTGCCA AAGGGGTTCT GAATGAGAAT 
AGCCTTCAGG CTGAATTCAG AAAGGTCCAG GGAAAACTGA AGAATGCCCG CAATATCATC 
AGCCTCCTCA AAGAACAGCT GGTGTTGAGC AGCAAGGAAG GGACTAGTAA GCTTAATCCT 
GAGCTCCTTG TGCACCTGGC CAAGGAGATC AACAGAATGA ACACAGAGCT GGTTTACTCT 
CCTGGAAAGC ACCAATGCCT AGAAGAGGAG GGTGTGACCA CAAGGCCGTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TGCCCTCACA GTGGATGCTC ACCAACTGGA TAACCAGCCC 
CAGACCCATG ACCCTGGGCC GCAGTCAGAA TTTAGCTTCT CAGGATCCAC GAAGCACCTG 
CGCTCCCAGT TGGCACAATG CAAACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCAGAAGCCA CTGTCTTTGC CCAGGCCAAC CAGCTGGAGA AATACAGAGT CATGTTTACA 
GGTGGATCGT CGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGACCTGGGC 
TATGAGACCT GTGGCCGAAG CGAGAACGAG GCTGAGCGGG AGGAGAGCAC CAGCCCTGAG 
TGTGAGGAGC ACGACAGCCT CAGGGAAACA GTCTTGATGG AGGGGCTGTG CTCCAAGCAG 
GGACACCTGG ACTCCACAAT GACCAGCCCC CCTGGGAAGA AGCCCCTGGA GAGCCAACCA 
GGGAAGCAGG AGGAGCTCCG GGCGTATGGA AAGTCAGAAG ACATCTCTGT CCTACGTAAG 
GACATCAAAG ATCTGAAGGC CCAGCTGCAG AACGCCAACA AGGTCATTCA GAACCTGAAG 
AGCCGGGTTC GGTCCCTCTC AGTTACAAGC GATTATTCAT CTAGTCTGGA GAGACCCCGA 
AAGCTGAAGG CCGTTGGTGC CCTAGAAGGA ACTTCACCGC ACAGTGTCAC TGATGAGGAC 
GAGGGGTGGC TGTCTGATGG CACTGGGGCT TTCTACCCTC CAGGGCTTCA GGCTAAAAAG 
GATCTGGAGA GTCTGATCCA GAGGGTGTCC CAGCTAGAAG CCCAGCTCCC AAAAACTGGA 
ACGGAAGGGA AGCTGGCCGA GGAGCTGAGA TCTGCTTCGT GGCCTGGGAA ATATGATTCC 
CTGATTCAGG ACCAGGCCCG AGAACTGTCC TACCTACGTC AGAAAATACG GGAAGGGAGA 
GGTATCTGTT ATCTTCTCAC CCAGCACTCG AAAGACACAG TAAAGTCTTT CGAGGACCTC 
CTAAGGAGCA ATGACATTGA CTACTACCTG GGACAGAGCT TCCGGGAGCA CCTCGCCCAG 
GGCAGCCAGC TGACAGAAAG GCTCACCAGC AAACTCAGCA CCAAGGATCA TAAAAGTGAG 
AAAGATCAAG CTGGACTCGA GCCACTGGCT CTGAGGCTTA GCAGGGAGCT GCAGGAGAAG 
GAGAAAGTGA TCGAAGTCCT GCAGGCCAAG CTGGACGCCC GCTCCCTCAC ACCGTGCAGC 
AGCCATGCCT TGTCAGACTC TCAGCGCTCT CCCAGCAGCT CCTCCTTCTT GTCTGACGAG 
CTGGAAGCCT GCTCTGACAT GGACATCGCC AGCGAGTACA CACACTATGA AGAGAAGAAG 
GCTTCACCTG GTCACTCAGA TTCCATCCAT CATTCGAGTC ATTCTGCTGT ACTGTCTTCC 
AAACCATCAG CAACCAGTTC ATCTCAGGAG GTTAAGGCCG AACCCAGCAG CAACCCCGTC 
AGCTTCCCAG CTCCCCAGAA TCCCCCCAAG GAGGCCAGCC AGGCCCATCC AGGCCTTCAT 
TTCCAATCCA TACCCACGCT GGTTAGCCTT CCCCAGGCAC CACTGCCCTC AGCTCCACCC 
AGCTTCCTGC CTTTCAGCTC TGCTGGCCCT CCCCTCCCTG GCTGCTGTGA GACACCCATG 
GTCTCCTTGG CAGAGGCTCA GCAGGAGCTG CAGATGCTGC GGAAGCAGTT AGGAGAAAGT 
GTCACCAGTG TTCATCCTGC TTCCCCAGCC ACACTGCCAA CCAACCACAC AGAAGCCAGA 
TCTTCCCACT ACCTCAACCC TCCCCAGCCC CTCTGTCCTC CAAGGGGCAC CACGGACCTG 
GGCAGAATCC TGGAGCCTGG GTACCTGGGT AGCAGTGGCC AGTGGGACGT GATGAGGCCT 
CAGAAAGGGA GCATATCCGG GGACCTATCC TCAGGCTCCT CCATGTACCA GCTTAACTCC 
AAGCCCACAG GGGCTGACCT GCTAGAGGAG CATCTTGGTG AAATCCGGAA CCTACGCCAG 
CGCCTGGAGG AGTCCATCTG CATTAATGAC CGCCTGCGGG AGCAGCTGGA AAACAGGCTC 
AGCTCCACTG CCCGCGAAAG TGGCTCAACC TCTAACTTCT ACAGTCCAGG CCTGGAGTCC 
ACACCTCAGC TCTCCAATGA GAACAGAGTC CTTAGGGAAG AAAACCACAG GCTTCAGGCT 
CAGCTCAGTC ATGTTTCCAG AGAGCACTCC CAGGAAACAG AATGCCTGAG GGAGGCTCTG 
CTGACCTCTC GATCCCGGCT TCAAGAGCTG GAAATGGAGC TGGAGCACCA AAAGGTAGAC 
CGGCAGCAGT TACTGGAAGA CCTGAAGGAG AAGCAGCAGG AGATCTTGCA TTTCCAGGAG 
GAACGACTGT CTCTCCAGGA GAAAGACTCC AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG 
CAACAGTGCG AAGAGAAACA GCAGCTCTTC CAGTCCCTCC AGTCAGAGCT ACAGATCTAT 
GAGGCACTTT ATGGTGACTC CAAGAAGACG CTGAAAGCTT ATACCTGGGA TGCCTGTCAC 
CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC CTGGTGGCAG AGATCCGAGC TTTGCGAGGG 
CAGCTGGAGC AGAGCATTCA GGTGAACAAC TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG 
GATGGTGGTG CCAGCAAAGC TGGCCTCAGC TCCTCCTCCG TTAACCAGAA ATTCCCCACC 
AACACTGACC CTGGAAACAA GCAGCCATTC TTCCAAGATT CGGTTGCCTC CCCTCCAGTT 
CGGGATGTGG GCATGAATTC TCCAGTTCTC GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT 
GGCCCAGAGA CCACAACCTT CAGTAGGACG AACGAGCTGG GTTTGGATGC TTCTCCAGTA 
ATGAAGAACC CTCGCAAGCT GGAGGGCGAC GCTACTGATG GCTCCTTTGC CAATAAGCAT 
GGCCGCCACG TCATTGGCCA CATTGATGAC TATAGTGCCC TAAGAGAGCA GATTGGGGAG 
GGCAGACTGC TGGTCAAGAA GATAGCATCA CTGGTGAGAC CAACCTGCAG CTTCTCTGGT 
CTTGAAGCTC CAGGCATAGA GCCACAGGGA TGCCCATGGC TACTGCCCCT TTCCCAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015463433.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318919.1 Bos taurus myomegalin (LOC508547), transcript variant X16, mRNA.

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CloneID	OBa89537
Clone ID Related Accession (Same CDS sequence)	XM_015462611.1 , XM_015463433.1 , XM_015463433.2
Accession Version	XM_015463433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC ACCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAC TTCCTGGAGG AGCGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGATGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGAG AACTCCAGGA CAAGAGACAG CATCCGGATA AAACATGCGC TGATGCAGAG 
AATATCAACA GCCATAATGA AGCTGAGCTC CGGCGCCAGT TTGAGGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAAGA GGAGTCCAGA 
CTAGCAAAAA ATGAAGCCGC ACGGATGGCG GCTCTGGTGG AAGCAGAGAA AGAGTGTAAC 
CTGGAGCTCT CAGAGAAACT GAAGGGAGTC ACCAAGGAGA GGGAAGATGT ACCAGGAGAC 
CGGGTCAAAT CTGACCAGTA CACTGAGGCC CTGGCCCAGA GGGACAAAAA AATTGAAGAA 
CTGAGTCAAA GCCTGGCTGC ACAGGAGAAA CTTGTAGAGC AGCTGTCTCA AGAGAAACAA 
CAACTGCTAC ATCTGTTGGA GGAGCCAGCT GACATGGAAG TGCAGCCCAT GACTGAAGAC 
TTACTCAAAC AACAGAAGTT GAATTCAAAT GAGACCGGTA CAACTCAGCA GTCTGTATCT 
GATTCCCATC TGGCAGAACT CCAGGAAAAA ATCCAGCAAA CAGAGACCAC CAACAAGATG 
CTTCAAGAAA AACTGAATGA AATGAGCTAT GAACTGAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG ATACAATTCA AAACCTCAAG GAAACTCTGA AAAGCAGGGA AAGTGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AACGACACAA TGGCTAAGCT TCGAGAAATG 
CTGCACCAGA GTCAGCTTAA ACAGCTTCAT AACTCAGAGG GTACTTCCCC AGCTCAGCAA 
CAGGTGGCTC TGCTTGATCT TCAAAGTGCT TTATTCTGCA GCCAGCTTGA AATACAGAAG 
CTCCAGAGGG CAGTACGCCA AAAAGAAAGG CAGCTGGCTG ATGCTAGACG ATGTGTGCGG 
TTTGTAGAGG CTGCAGCACA GGAGAGAGAA CAGCAGAAAG AGGCTTCTTG GAAACATAAC 
CAAGAATTGC AAAAAGCCCT ACAGCAGCTA CAAGGAGAAC TTCAGAGTAA GAGCCAACAG 
CTTCATACCC TGGAGGCTGA AAAATATAAT GAGATTCGCA CCCAAGAGCA GCACATTCAG 
CACCTCCACT ACAGTCTGAG TCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTACCGAA ATAACTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
AGGCAAAGAA TACAAGATAG AGATGTTGCC CTAGAGCGGG CTATAGATGA AAAGTTCTCC 
AGCCTAGAAG AAAAGGAAAA AGAACTGCGT CAGCTTCACC TTGCCATGAG AGAGCGAGAT 
CACGACTTAG AGAGGCTGCG CGGTATCCTC TCCGCCAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGTCCAA AGGCCTGGAA GTGGAACAGT TGTCTGCCAC CTGTCAGAAC 
CTCCAGTGGC TGAAAGAAGA AATGGAAACC AAATTTAGCC GTTGGCAGAA GGAGCAAGAA 
AGCATCATTC AGCAGCTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTT 
ACCGCAACAT TGCTCTGCAA ACTTGGACCA GGGCAGACTG AGATAGTTGA GGAGCTGTGC 
CAGCGTCTAC AGCAGAAGGA AAGGATGCTG CAGGACCTTC TGAGTGATCG GAACAAGCAA 
GCAGTGGAAT ATGAAATGGA GATTCAGGGC CTGCTTCAGT CCATGAGCAC CAGAGAGCAG 
GAGAGCCACG CTGCTGCAGA AAAGATGGTA CAAGCTCTAA TGGAAAGAAA TTCAGAATTA 
CAGGCCCTGC GCCAATATTT AGGAGGGAAA GATTTCATGA TGTCCCAAGC ACCCCTCTCT 
AACCGACCAG CTGAAGTTAC CTCCATCAGC CCCCATCTTG GAGAGCAAAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAG AGATGATAGC ACTTCATTGA CTGCCAAAGG GGATGCTAGC 
GTACCCAGGT CCTCGTTAGG GGAGGTGGAT ACAGTTGCGG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAACTTGA GCTCATGGCT AAAAAAGAGA GAGAAAGTCG GATGGAACTG 
TCTGCACTCC AGTCCATGGT GGCTGTGCAG GAGCAAGAGC TGCAGGTTCA GGCTGCTGAC 
ATGGAGTCCC TGACCAGGAC CATACAGATT AAGGAAGACC TCATAAAGGA CCTGCAGATG 
CAGCTGGTTG ATCCTGAAGA CATCCCAGCT ATGGAACGCC TGACCCAGGA AGTCTTGCTT 
CTTCGGGAAA AAGTGGCTTC AGTAGAATCA CAAGGACAGG AAACGTCTGG AAACCGAAGA 
CAACAATTGC TGCTGATGCT CGAAGGACTG GTAGATGAGC GGAGTCGGCT CAATGAGGCC 
TTACAGGCAG AGAGACAGCT CTATAGCAGT CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGCAATCGG 
CTAGAAGAAG TTCTCGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCCGCC 
ATTGGAGGTA CAGCTGCAGG GGACGACACG GAGGATGCGA GCACGGAGTT TACCGACAGT 
ATCGAGGAGG AGGCTGCACA CAACAGCCAC CAGCAACTCA TCAAGGTGGC GTTGGAGAAA 
AGCCGGGCAG CTGTGGAGAC CCAGAATGTG TCTCCTGCTC CTCCCACCCT GACAGGAGGG 
GACGGTAACA GGGGTCTTCA GGAGGAAATG CTCCACCTGA GGGCTGAAAT TCACCGGCAC 
TTAGAGGAGA AGAGGAAAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT TGAGGAAGAC 
AAGGAGAAGG GCGAGGCGAT GGTGGAGACT GTGGTTGCCA AAGGGGTTCT GAATGAGAAT 
AGCCTTCAGG CTGAATTCAG AAAGGTCCAG GGAAAACTGA AGAATGCCCG CAATATCATC 
AGCCTCCTCA AAGAACAGCT GGTGTTGAGC AGCAAGGAAG GGACTAGTAA GCTTAATCCT 
GAGCTCCTTG TGCACCTGGC CAAGGAGATC AACAGAATGA ACACAGAGCT GGTTTACTCT 
CCTGGAAAGC ACCAATGCCT AGAAGAGGAG GGTGTGACCA CAAGGCCGTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TGCCCTCACA GTGGATGCTC ACCAACTGGA TAACCAGCCC 
CAGACCCATG ACCCTGGGCC GCAGTCAGAA TTTAGCTTCT CAGGATCCAC GAAGCACCTG 
CGCTCCCAGT TGGCACAATG CAAACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCAGAAGCCA CTGTCTTTGC CCAGGCCAAC CAGCTGGAGA AATACAGAGT CATGTTTACA 
GGTGGATCGT CGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGACCTGGGC 
TATGAGACCT GTGGCCGAAG CGAGAACGAG GCTGAGCGGG AGGAGAGCAC CAGCCCTGAG 
TGTGAGGAGC ACGACAGCCT CAGGGAAACA GTCTTGATGG AGGGGCTGTG CTCCAAGCAG 
GGACACCTGG ACTCCACAAT GACCAGCCCC CCTGGGAAGA AGCCCCTGGA GAGCCAACCA 
GGGAAGCAGG AGGAGCTCCG GGCGTATGGA AAGTCAGAAG ACATCTCTGT CCTACGTAAG 
GACATCAAAG ATCTGAAGGC CCAGCTGCAG AACGCCAACA AGGTCATTCA GAACCTGAAG 
AGCCGGGTTC GGTCCCTCTC AGTTACAAGC GATTATTCAT CTAGTCTGGA GAGACCCCGA 
AAGCTGAAGG CCGTTGGTGC CCTAGAAGGA ACTTCACCGC ACAGTGTCAC TGATGAGGAC 
GAGGGGTGGC TGTCTGATGG CACTGGGGCT TTCTACCCTC CAGGGCTTCA GGCTAAAAAG 
GATCTGGAGA GTCTGATCCA GAGGGTGTCC CAGCTAGAAG CCCAGCTCCC AAAAACTGGA 
ACGGAAGGGA AGCTGGCCGA GGAGCTGAGA TCTGCTTCGT GGCCTGGGAA ATATGATTCC 
CTGATTCAGG ACCAGGCCCG AGAACTGTCC TACCTACGTC AGAAAATACG GGAAGGGAGA 
GGTATCTGTT ATCTTCTCAC CCAGCACTCG AAAGACACAG TAAAGTCTTT CGAGGACCTC 
CTAAGGAGCA ATGACATTGA CTACTACCTG GGACAGAGCT TCCGGGAGCA CCTCGCCCAG 
GGCAGCCAGC TGACAGAAAG GCTCACCAGC AAACTCAGCA CCAAGGATCA TAAAAGTGAG 
AAAGATCAAG CTGGACTCGA GCCACTGGCT CTGAGGCTTA GCAGGGAGCT GCAGGAGAAG 
GAGAAAGTGA TCGAAGTCCT GCAGGCCAAG CTGGACGCCC GCTCCCTCAC ACCGTGCAGC 
AGCCATGCCT TGTCAGACTC TCAGCGCTCT CCCAGCAGCT CCTCCTTCTT GTCTGACGAG 
CTGGAAGCCT GCTCTGACAT GGACATCGCC AGCGAGTACA CACACTATGA AGAGAAGAAG 
GCTTCACCTG GTCACTCAGA TTCCATCCAT CATTCGAGTC ATTCTGCTGT ACTGTCTTCC 
AAACCATCAG CAACCAGTTC ATCTCAGGAG GTTAAGGCCG AACCCAGCAG CAACCCCGTC 
AGCTTCCCAG CTCCCCAGAA TCCCCCCAAG GAGGCCAGCC AGGCCCATCC AGGCCTTCAT 
TTCCAATCCA TACCCACGCT GGTTAGCCTT CCCCAGGCAC CACTGCCCTC AGCTCCACCC 
AGCTTCCTGC CTTTCAGCTC TGCTGGCCCT CCCCTCCCTG GCTGCTGTGA GACACCCATG 
GTCTCCTTGG CAGAGGCTCA GCAGGAGCTG CAGATGCTGC GGAAGCAGTT AGGAGAAAGT 
GTCACCAGTG TTCATCCTGC TTCCCCAGCC ACACTGCCAA CCAACCACAC AGAAGCCAGA 
TCTTCCCACT ACCTCAACCC TCCCCAGCCC CTCTGTCCTC CAAGGGGCAC CACGGACCTG 
GGCAGAATCC TGGAGCCTGG GTACCTGGGT AGCAGTGGCC AGTGGGACGT GATGAGGCCT 
CAGAAAGGGA GCATATCCGG GGACCTATCC TCAGGCTCCT CCATGTACCA GCTTAACTCC 
AAGCCCACAG GGGCTGACCT GCTAGAGGAG CATCTTGGTG AAATCCGGAA CCTACGCCAG 
CGCCTGGAGG AGTCCATCTG CATTAATGAC CGCCTGCGGG AGCAGCTGGA AAACAGGCTC 
AGCTCCACTG CCCGCGAAAG TGGCTCAACC TCTAACTTCT ACAGTCCAGG CCTGGAGTCC 
ACACCTCAGC TCTCCAATGA GAACAGAGTC CTTAGGGAAG AAAACCACAG GCTTCAGGCT 
CAGCTCAGTC ATGTTTCCAG AGAGCACTCC CAGGAAACAG AATGCCTGAG GGAGGCTCTG 
CTGACCTCTC GATCCCGGCT TCAAGAGCTG GAAATGGAGC TGGAGCACCA AAAGGTAGAC 
CGGCAGCAGT TACTGGAAGA CCTGAAGGAG AAGCAGCAGG AGATCTTGCA TTTCCAGGAG 
GAACGACTGT CTCTCCAGGA GAAAGACTCC AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG 
CAACAGTGCG AAGAGAAACA GCAGCTCTTC CAGTCCCTCC AGTCAGAGCT ACAGATCTAT 
GAGGCACTTT ATGGTGACTC CAAGAAGACG CTGAAAGCTT ATACCTGGGA TGCCTGTCAC 
CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC CTGGTGGCAG AGATCCGAGC TTTGCGAGGG 
CAGCTGGAGC AGAGCATTCA GGTGAACAAC TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG 
GATGGTGGTG CCAGCAAAGC TGGCCTCAGC TCCTCCTCCG TTAACCAGAA ATTCCCCACC 
AACACTGACC CTGGAAACAA GCAGCCATTC TTCCAAGATT CGGTTGCCTC CCCTCCAGTT 
CGGGATGTGG GCATGAATTC TCCAGTTCTC GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT 
GGCCCAGAGA CCACAACCTT CAGTAGGACG AACGAGCTGG GTTTGGATGC TTCTCCAGTA 
ATGAAGAACC CTCGCAAGCT GGAGGGCGAC GCTACTGATG GCTCCTTTGC CAATAAGCAT 
GGCCGCCACG TCATTGGCCA CATTGATGAC TATAGTGCCC TAAGAGAGCA GATTGGGGAG 
GGCAGACTGC TGGTCAAGAA GATAGCATCA CTGGTGAGAC CAACCTGCAG CTTCTCTGGT 
CTTGAAGCTC CAGGCATAGA GCCACAGGGA TGCCCATGGC TACTGCCCCT TTCCCAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318919.1
CDS	323..7567
Translation	MMGRWYPEQVMQQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015463433.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463433.1

ORF Insert Sequence:

ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC ACCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAC TTCCTGGAGG AGCGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGATGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGAG AACTCCAGGA CAAGAGACAG CATCCGGATA AAACATGCGC TGATGCAGAG 
AATATCAACA GCCATAATGA AGCTGAGCTC CGGCGCCAGT TTGAGGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAAGA GGAGTCCAGA 
CTAGCAAAAA ATGAAGCCGC ACGGATGGCG GCTCTGGTGG AAGCAGAGAA AGAGTGTAAC 
CTGGAGCTCT CAGAGAAACT GAAGGGAGTC ACCAAGGAGA GGGAAGATGT ACCAGGAGAC 
CGGGTCAAAT CTGACCAGTA CACTGAGGCC CTGGCCCAGA GGGACAAAAA AATTGAAGAA 
CTGAGTCAAA GCCTGGCTGC ACAGGAGAAA CTTGTAGAGC AGCTGTCTCA AGAGAAACAA 
CAACTGCTAC ATCTGTTGGA GGAGCCAGCT GACATGGAAG TGCAGCCCAT GACTGAAGAC 
TTACTCAAAC AACAGAAGTT GAATTCAAAT GAGACCGGTA CAACTCAGCA GTCTGTATCT 
GATTCCCATC TGGCAGAACT CCAGGAAAAA ATCCAGCAAA CAGAGACCAC CAACAAGATG 
CTTCAAGAAA AACTGAATGA AATGAGCTAT GAACTGAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG ATACAATTCA AAACCTCAAG GAAACTCTGA AAAGCAGGGA AAGTGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AACGACACAA TGGCTAAGCT TCGAGAAATG 
CTGCACCAGA GTCAGCTTAA ACAGCTTCAT AACTCAGAGG GTACTTCCCC AGCTCAGCAA 
CAGGTGGCTC TGCTTGATCT TCAAAGTGCT TTATTCTGCA GCCAGCTTGA AATACAGAAG 
CTCCAGAGGG CAGTACGCCA AAAAGAAAGG CAGCTGGCTG ATGCTAGACG ATGTGTGCGG 
TTTGTAGAGG CTGCAGCACA GGAGAGAGAA CAGCAGAAAG AGGCTTCTTG GAAACATAAC 
CAAGAATTGC AAAAAGCCCT ACAGCAGCTA CAAGGAGAAC TTCAGAGTAA GAGCCAACAG 
CTTCATACCC TGGAGGCTGA AAAATATAAT GAGATTCGCA CCCAAGAGCA GCACATTCAG 
CACCTCCACT ACAGTCTGAG TCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTACCGAA ATAACTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
AGGCAAAGAA TACAAGATAG AGATGTTGCC CTAGAGCGGG CTATAGATGA AAAGTTCTCC 
AGCCTAGAAG AAAAGGAAAA AGAACTGCGT CAGCTTCACC TTGCCATGAG AGAGCGAGAT 
CACGACTTAG AGAGGCTGCG CGGTATCCTC TCCGCCAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGTCCAA AGGCCTGGAA GTGGAACAGT TGTCTGCCAC CTGTCAGAAC 
CTCCAGTGGC TGAAAGAAGA AATGGAAACC AAATTTAGCC GTTGGCAGAA GGAGCAAGAA 
AGCATCATTC AGCAGCTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTT 
ACCGCAACAT TGCTCTGCAA ACTTGGACCA GGGCAGACTG AGATAGTTGA GGAGCTGTGC 
CAGCGTCTAC AGCAGAAGGA AAGGATGCTG CAGGACCTTC TGAGTGATCG GAACAAGCAA 
GCAGTGGAAT ATGAAATGGA GATTCAGGGC CTGCTTCAGT CCATGAGCAC CAGAGAGCAG 
GAGAGCCACG CTGCTGCAGA AAAGATGGTA CAAGCTCTAA TGGAAAGAAA TTCAGAATTA 
CAGGCCCTGC GCCAATATTT AGGAGGGAAA GATTTCATGA TGTCCCAAGC ACCCCTCTCT 
AACCGACCAG CTGAAGTTAC CTCCATCAGC CCCCATCTTG GAGAGCAAAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAG AGATGATAGC ACTTCATTGA CTGCCAAAGG GGATGCTAGC 
GTACCCAGGT CCTCGTTAGG GGAGGTGGAT ACAGTTGCGG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAACTTGA GCTCATGGCT AAAAAAGAGA GAGAAAGTCG GATGGAACTG 
TCTGCACTCC AGTCCATGGT GGCTGTGCAG GAGCAAGAGC TGCAGGTTCA GGCTGCTGAC 
ATGGAGTCCC TGACCAGGAC CATACAGATT AAGGAAGACC TCATAAAGGA CCTGCAGATG 
CAGCTGGTTG ATCCTGAAGA CATCCCAGCT ATGGAACGCC TGACCCAGGA AGTCTTGCTT 
CTTCGGGAAA AAGTGGCTTC AGTAGAATCA CAAGGACAGG AAACGTCTGG AAACCGAAGA 
CAACAATTGC TGCTGATGCT CGAAGGACTG GTAGATGAGC GGAGTCGGCT CAATGAGGCC 
TTACAGGCAG AGAGACAGCT CTATAGCAGT CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGCAATCGG 
CTAGAAGAAG TTCTCGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCCGCC 
ATTGGAGGTA CAGCTGCAGG GGACGACACG GAGGATGCGA GCACGGAGTT TACCGACAGT 
ATCGAGGAGG AGGCTGCACA CAACAGCCAC CAGCAACTCA TCAAGGTGGC GTTGGAGAAA 
AGCCGGGCAG CTGTGGAGAC CCAGAATGTG TCTCCTGCTC CTCCCACCCT GACAGGAGGG 
GACGGTAACA GGGGTCTTCA GGAGGAAATG CTCCACCTGA GGGCTGAAAT TCACCGGCAC 
TTAGAGGAGA AGAGGAAAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT TGAGGAAGAC 
AAGGAGAAGG GCGAGGCGAT GGTGGAGACT GTGGTTGCCA AAGGGGTTCT GAATGAGAAT 
AGCCTTCAGG CTGAATTCAG AAAGGTCCAG GGAAAACTGA AGAATGCCCG CAATATCATC 
AGCCTCCTCA AAGAACAGCT GGTGTTGAGC AGCAAGGAAG GGACTAGTAA GCTTAATCCT 
GAGCTCCTTG TGCACCTGGC CAAGGAGATC AACAGAATGA ACACAGAGCT GGTTTACTCT 
CCTGGAAAGC ACCAATGCCT AGAAGAGGAG GGTGTGACCA CAAGGCCGTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TGCCCTCACA GTGGATGCTC ACCAACTGGA TAACCAGCCC 
CAGACCCATG ACCCTGGGCC GCAGTCAGAA TTTAGCTTCT CAGGATCCAC GAAGCACCTG 
CGCTCCCAGT TGGCACAATG CAAACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCAGAAGCCA CTGTCTTTGC CCAGGCCAAC CAGCTGGAGA AATACAGAGT CATGTTTACA 
GGTGGATCGT CGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGACCTGGGC 
TATGAGACCT GTGGCCGAAG CGAGAACGAG GCTGAGCGGG AGGAGAGCAC CAGCCCTGAG 
TGTGAGGAGC ACGACAGCCT CAGGGAAACA GTCTTGATGG AGGGGCTGTG CTCCAAGCAG 
GGACACCTGG ACTCCACAAT GACCAGCCCC CCTGGGAAGA AGCCCCTGGA GAGCCAACCA 
GGGAAGCAGG AGGAGCTCCG GGCGTATGGA AAGTCAGAAG ACATCTCTGT CCTACGTAAG 
GACATCAAAG ATCTGAAGGC CCAGCTGCAG AACGCCAACA AGGTCATTCA GAACCTGAAG 
AGCCGGGTTC GGTCCCTCTC AGTTACAAGC GATTATTCAT CTAGTCTGGA GAGACCCCGA 
AAGCTGAAGG CCGTTGGTGC CCTAGAAGGA ACTTCACCGC ACAGTGTCAC TGATGAGGAC 
GAGGGGTGGC TGTCTGATGG CACTGGGGCT TTCTACCCTC CAGGGCTTCA GGCTAAAAAG 
GATCTGGAGA GTCTGATCCA GAGGGTGTCC CAGCTAGAAG CCCAGCTCCC AAAAACTGGA 
ACGGAAGGGA AGCTGGCCGA GGAGCTGAGA TCTGCTTCGT GGCCTGGGAA ATATGATTCC 
CTGATTCAGG ACCAGGCCCG AGAACTGTCC TACCTACGTC AGAAAATACG GGAAGGGAGA 
GGTATCTGTT ATCTTCTCAC CCAGCACTCG AAAGACACAG TAAAGTCTTT CGAGGACCTC 
CTAAGGAGCA ATGACATTGA CTACTACCTG GGACAGAGCT TCCGGGAGCA CCTCGCCCAG 
GGCAGCCAGC TGACAGAAAG GCTCACCAGC AAACTCAGCA CCAAGGATCA TAAAAGTGAG 
AAAGATCAAG CTGGACTCGA GCCACTGGCT CTGAGGCTTA GCAGGGAGCT GCAGGAGAAG 
GAGAAAGTGA TCGAAGTCCT GCAGGCCAAG CTGGACGCCC GCTCCCTCAC ACCGTGCAGC 
AGCCATGCCT TGTCAGACTC TCAGCGCTCT CCCAGCAGCT CCTCCTTCTT GTCTGACGAG 
CTGGAAGCCT GCTCTGACAT GGACATCGCC AGCGAGTACA CACACTATGA AGAGAAGAAG 
GCTTCACCTG GTCACTCAGA TTCCATCCAT CATTCGAGTC ATTCTGCTGT ACTGTCTTCC 
AAACCATCAG CAACCAGTTC ATCTCAGGAG GTTAAGGCCG AACCCAGCAG CAACCCCGTC 
AGCTTCCCAG CTCCCCAGAA TCCCCCCAAG GAGGCCAGCC AGGCCCATCC AGGCCTTCAT 
TTCCAATCCA TACCCACGCT GGTTAGCCTT CCCCAGGCAC CACTGCCCTC AGCTCCACCC 
AGCTTCCTGC CTTTCAGCTC TGCTGGCCCT CCCCTCCCTG GCTGCTGTGA GACACCCATG 
GTCTCCTTGG CAGAGGCTCA GCAGGAGCTG CAGATGCTGC GGAAGCAGTT AGGAGAAAGT 
GTCACCAGTG TTCATCCTGC TTCCCCAGCC ACACTGCCAA CCAACCACAC AGAAGCCAGA 
TCTTCCCACT ACCTCAACCC TCCCCAGCCC CTCTGTCCTC CAAGGGGCAC CACGGACCTG 
GGCAGAATCC TGGAGCCTGG GTACCTGGGT AGCAGTGGCC AGTGGGACGT GATGAGGCCT 
CAGAAAGGGA GCATATCCGG GGACCTATCC TCAGGCTCCT CCATGTACCA GCTTAACTCC 
AAGCCCACAG GGGCTGACCT GCTAGAGGAG CATCTTGGTG AAATCCGGAA CCTACGCCAG 
CGCCTGGAGG AGTCCATCTG CATTAATGAC CGCCTGCGGG AGCAGCTGGA AAACAGGCTC 
AGCTCCACTG CCCGCGAAAG TGGCTCAACC TCTAACTTCT ACAGTCCAGG CCTGGAGTCC 
ACACCTCAGC TCTCCAATGA GAACAGAGTC CTTAGGGAAG AAAACCACAG GCTTCAGGCT 
CAGCTCAGTC ATGTTTCCAG AGAGCACTCC CAGGAAACAG AATGCCTGAG GGAGGCTCTG 
CTGACCTCTC GATCCCGGCT TCAAGAGCTG GAAATGGAGC TGGAGCACCA AAAGGTAGAC 
CGGCAGCAGT TACTGGAAGA CCTGAAGGAG AAGCAGCAGG AGATCTTGCA TTTCCAGGAG 
GAACGACTGT CTCTCCAGGA GAAAGACTCC AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG 
CAACAGTGCG AAGAGAAACA GCAGCTCTTC CAGTCCCTCC AGTCAGAGCT ACAGATCTAT 
GAGGCACTTT ATGGTGACTC CAAGAAGACG CTGAAAGCTT ATACCTGGGA TGCCTGTCAC 
CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC CTGGTGGCAG AGATCCGAGC TTTGCGAGGG 
CAGCTGGAGC AGAGCATTCA GGTGAACAAC TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG 
GATGGTGGTG CCAGCAAAGC TGGCCTCAGC TCCTCCTCCG TTAACCAGAA ATTCCCCACC 
AACACTGACC CTGGAAACAA GCAGCCATTC TTCCAAGATT CGGTTGCCTC CCCTCCAGTT 
CGGGATGTGG GCATGAATTC TCCAGTTCTC GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT 
GGCCCAGAGA CCACAACCTT CAGTAGGACG AACGAGCTGG GTTTGGATGC TTCTCCAGTA 
ATGAAGAACC CTCGCAAGCT GGAGGGCGAC GCTACTGATG GCTCCTTTGC CAATAAGCAT 
GGCCGCCACG TCATTGGCCA CATTGATGAC TATAGTGCCC TAAGAGAGCA GATTGGGGAG 
GGCAGACTGC TGGTCAAGAA GATAGCATCA CTGGTGAGAC CAACCTGCAG CTTCTCTGGT 
CTTGAAGCTC CAGGCATAGA GCCACAGGGA TGCCCATGGC TACTGCCCCT TTCCCAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015463433.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318919.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X24, mRNA.

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CloneID	OBa89537
Clone ID Related Accession (Same CDS sequence)	XM_015462611.1 , XM_015463433.1 , XM_015463433.2
Accession Version	XM_015463433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015463433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC ACCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAC TTCCTGGAGG AGCGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGATGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGAG AACTCCAGGA CAAGAGACAG CATCCGGATA AAACATGCGC TGATGCAGAG 
AATATCAACA GCCATAATGA AGCTGAGCTC CGGCGCCAGT TTGAGGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAAGA GGAGTCCAGA 
CTAGCAAAAA ATGAAGCCGC ACGGATGGCG GCTCTGGTGG AAGCAGAGAA AGAGTGTAAC 
CTGGAGCTCT CAGAGAAACT GAAGGGAGTC ACCAAGGAGA GGGAAGATGT ACCAGGAGAC 
CGGGTCAAAT CTGACCAGTA CACTGAGGCC CTGGCCCAGA GGGACAAAAA AATTGAAGAA 
CTGAGTCAAA GCCTGGCTGC ACAGGAGAAA CTTGTAGAGC AGCTGTCTCA AGAGAAACAA 
CAACTGCTAC ATCTGTTGGA GGAGCCAGCT GACATGGAAG TGCAGCCCAT GACTGAAGAC 
TTACTCAAAC AACAGAAGTT GAATTCAAAT GAGACCGGTA CAACTCAGCA GTCTGTATCT 
GATTCCCATC TGGCAGAACT CCAGGAAAAA ATCCAGCAAA CAGAGACCAC CAACAAGATG 
CTTCAAGAAA AACTGAATGA AATGAGCTAT GAACTGAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG ATACAATTCA AAACCTCAAG GAAACTCTGA AAAGCAGGGA AAGTGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AACGACACAA TGGCTAAGCT TCGAGAAATG 
CTGCACCAGA GTCAGCTTAA ACAGCTTCAT AACTCAGAGG GTACTTCCCC AGCTCAGCAA 
CAGGTGGCTC TGCTTGATCT TCAAAGTGCT TTATTCTGCA GCCAGCTTGA AATACAGAAG 
CTCCAGAGGG CAGTACGCCA AAAAGAAAGG CAGCTGGCTG ATGCTAGACG ATGTGTGCGG 
TTTGTAGAGG CTGCAGCACA GGAGAGAGAA CAGCAGAAAG AGGCTTCTTG GAAACATAAC 
CAAGAATTGC AAAAAGCCCT ACAGCAGCTA CAAGGAGAAC TTCAGAGTAA GAGCCAACAG 
CTTCATACCC TGGAGGCTGA AAAATATAAT GAGATTCGCA CCCAAGAGCA GCACATTCAG 
CACCTCCACT ACAGTCTGAG TCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTACCGAA ATAACTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
AGGCAAAGAA TACAAGATAG AGATGTTGCC CTAGAGCGGG CTATAGATGA AAAGTTCTCC 
AGCCTAGAAG AAAAGGAAAA AGAACTGCGT CAGCTTCACC TTGCCATGAG AGAGCGAGAT 
CACGACTTAG AGAGGCTGCG CGGTATCCTC TCCGCCAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGTCCAA AGGCCTGGAA GTGGAACAGT TGTCTGCCAC CTGTCAGAAC 
CTCCAGTGGC TGAAAGAAGA AATGGAAACC AAATTTAGCC GTTGGCAGAA GGAGCAAGAA 
AGCATCATTC AGCAGCTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTT 
ACCGCAACAT TGCTCTGCAA ACTTGGACCA GGGCAGACTG AGATAGTTGA GGAGCTGTGC 
CAGCGTCTAC AGCAGAAGGA AAGGATGCTG CAGGACCTTC TGAGTGATCG GAACAAGCAA 
GCAGTGGAAT ATGAAATGGA GATTCAGGGC CTGCTTCAGT CCATGAGCAC CAGAGAGCAG 
GAGAGCCACG CTGCTGCAGA AAAGATGGTA CAAGCTCTAA TGGAAAGAAA TTCAGAATTA 
CAGGCCCTGC GCCAATATTT AGGAGGGAAA GATTTCATGA TGTCCCAAGC ACCCCTCTCT 
AACCGACCAG CTGAAGTTAC CTCCATCAGC CCCCATCTTG GAGAGCAAAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAG AGATGATAGC ACTTCATTGA CTGCCAAAGG GGATGCTAGC 
GTACCCAGGT CCTCGTTAGG GGAGGTGGAT ACAGTTGCGG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAACTTGA GCTCATGGCT AAAAAAGAGA GAGAAAGTCG GATGGAACTG 
TCTGCACTCC AGTCCATGGT GGCTGTGCAG GAGCAAGAGC TGCAGGTTCA GGCTGCTGAC 
ATGGAGTCCC TGACCAGGAC CATACAGATT AAGGAAGACC TCATAAAGGA CCTGCAGATG 
CAGCTGGTTG ATCCTGAAGA CATCCCAGCT ATGGAACGCC TGACCCAGGA AGTCTTGCTT 
CTTCGGGAAA AAGTGGCTTC AGTAGAATCA CAAGGACAGG AAACGTCTGG AAACCGAAGA 
CAACAATTGC TGCTGATGCT CGAAGGACTG GTAGATGAGC GGAGTCGGCT CAATGAGGCC 
TTACAGGCAG AGAGACAGCT CTATAGCAGT CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGCAATCGG 
CTAGAAGAAG TTCTCGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCCGCC 
ATTGGAGGTA CAGCTGCAGG GGACGACACG GAGGATGCGA GCACGGAGTT TACCGACAGT 
ATCGAGGAGG AGGCTGCACA CAACAGCCAC CAGCAACTCA TCAAGGTGGC GTTGGAGAAA 
AGCCGGGCAG CTGTGGAGAC CCAGAATGTG TCTCCTGCTC CTCCCACCCT GACAGGAGGG 
GACGGTAACA GGGGTCTTCA GGAGGAAATG CTCCACCTGA GGGCTGAAAT TCACCGGCAC 
TTAGAGGAGA AGAGGAAAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT TGAGGAAGAC 
AAGGAGAAGG GCGAGGCGAT GGTGGAGACT GTGGTTGCCA AAGGGGTTCT GAATGAGAAT 
AGCCTTCAGG CTGAATTCAG AAAGGTCCAG GGAAAACTGA AGAATGCCCG CAATATCATC 
AGCCTCCTCA AAGAACAGCT GGTGTTGAGC AGCAAGGAAG GGACTAGTAA GCTTAATCCT 
GAGCTCCTTG TGCACCTGGC CAAGGAGATC AACAGAATGA ACACAGAGCT GGTTTACTCT 
CCTGGAAAGC ACCAATGCCT AGAAGAGGAG GGTGTGACCA CAAGGCCGTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TGCCCTCACA GTGGATGCTC ACCAACTGGA TAACCAGCCC 
CAGACCCATG ACCCTGGGCC GCAGTCAGAA TTTAGCTTCT CAGGATCCAC GAAGCACCTG 
CGCTCCCAGT TGGCACAATG CAAACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCAGAAGCCA CTGTCTTTGC CCAGGCCAAC CAGCTGGAGA AATACAGAGT CATGTTTACA 
GGTGGATCGT CGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGACCTGGGC 
TATGAGACCT GTGGCCGAAG CGAGAACGAG GCTGAGCGGG AGGAGAGCAC CAGCCCTGAG 
TGTGAGGAGC ACGACAGCCT CAGGGAAACA GTCTTGATGG AGGGGCTGTG CTCCAAGCAG 
GGACACCTGG ACTCCACAAT GACCAGCCCC CCTGGGAAGA AGCCCCTGGA GAGCCAACCA 
GGGAAGCAGG AGGAGCTCCG GGCGTATGGA AAGTCAGAAG ACATCTCTGT CCTACGTAAG 
GACATCAAAG ATCTGAAGGC CCAGCTGCAG AACGCCAACA AGGTCATTCA GAACCTGAAG 
AGCCGGGTTC GGTCCCTCTC AGTTACAAGC GATTATTCAT CTAGTCTGGA GAGACCCCGA 
AAGCTGAAGG CCGTTGGTGC CCTAGAAGGA ACTTCACCGC ACAGTGTCAC TGATGAGGAC 
GAGGGGTGGC TGTCTGATGG CACTGGGGCT TTCTACCCTC CAGGGCTTCA GGCTAAAAAG 
GATCTGGAGA GTCTGATCCA GAGGGTGTCC CAGCTAGAAG CCCAGCTCCC AAAAACTGGA 
ACGGAAGGGA AGCTGGCCGA GGAGCTGAGA TCTGCTTCGT GGCCTGGGAA ATATGATTCC 
CTGATTCAGG ACCAGGCCCG AGAACTGTCC TACCTACGTC AGAAAATACG GGAAGGGAGA 
GGTATCTGTT ATCTTCTCAC CCAGCACTCG AAAGACACAG TAAAGTCTTT CGAGGACCTC 
CTAAGGAGCA ATGACATTGA CTACTACCTG GGACAGAGCT TCCGGGAGCA CCTCGCCCAG 
GGCAGCCAGC TGACAGAAAG GCTCACCAGC AAACTCAGCA CCAAGGATCA TAAAAGTGAG 
AAAGATCAAG CTGGACTCGA GCCACTGGCT CTGAGGCTTA GCAGGGAGCT GCAGGAGAAG 
GAGAAAGTGA TCGAAGTCCT GCAGGCCAAG CTGGACGCCC GCTCCCTCAC ACCGTGCAGC 
AGCCATGCCT TGTCAGACTC TCAGCGCTCT CCCAGCAGCT CCTCCTTCTT GTCTGACGAG 
CTGGAAGCCT GCTCTGACAT GGACATCGCC AGCGAGTACA CACACTATGA AGAGAAGAAG 
GCTTCACCTG GTCACTCAGA TTCCATCCAT CATTCGAGTC ATTCTGCTGT ACTGTCTTCC 
AAACCATCAG CAACCAGTTC ATCTCAGGAG GTTAAGGCCG AACCCAGCAG CAACCCCGTC 
AGCTTCCCAG CTCCCCAGAA TCCCCCCAAG GAGGCCAGCC AGGCCCATCC AGGCCTTCAT 
TTCCAATCCA TACCCACGCT GGTTAGCCTT CCCCAGGCAC CACTGCCCTC AGCTCCACCC 
AGCTTCCTGC CTTTCAGCTC TGCTGGCCCT CCCCTCCCTG GCTGCTGTGA GACACCCATG 
GTCTCCTTGG CAGAGGCTCA GCAGGAGCTG CAGATGCTGC GGAAGCAGTT AGGAGAAAGT 
GTCACCAGTG TTCATCCTGC TTCCCCAGCC ACACTGCCAA CCAACCACAC AGAAGCCAGA 
TCTTCCCACT ACCTCAACCC TCCCCAGCCC CTCTGTCCTC CAAGGGGCAC CACGGACCTG 
GGCAGAATCC TGGAGCCTGG GTACCTGGGT AGCAGTGGCC AGTGGGACGT GATGAGGCCT 
CAGAAAGGGA GCATATCCGG GGACCTATCC TCAGGCTCCT CCATGTACCA GCTTAACTCC 
AAGCCCACAG GGGCTGACCT GCTAGAGGAG CATCTTGGTG AAATCCGGAA CCTACGCCAG 
CGCCTGGAGG AGTCCATCTG CATTAATGAC CGCCTGCGGG AGCAGCTGGA AAACAGGCTC 
AGCTCCACTG CCCGCGAAAG TGGCTCAACC TCTAACTTCT ACAGTCCAGG CCTGGAGTCC 
ACACCTCAGC TCTCCAATGA GAACAGAGTC CTTAGGGAAG AAAACCACAG GCTTCAGGCT 
CAGCTCAGTC ATGTTTCCAG AGAGCACTCC CAGGAAACAG AATGCCTGAG GGAGGCTCTG 
CTGACCTCTC GATCCCGGCT TCAAGAGCTG GAAATGGAGC TGGAGCACCA AAAGGTAGAC 
CGGCAGCAGT TACTGGAAGA CCTGAAGGAG AAGCAGCAGG AGATCTTGCA TTTCCAGGAG 
GAACGACTGT CTCTCCAGGA GAAAGACTCC AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG 
CAACAGTGCG AAGAGAAACA GCAGCTCTTC CAGTCCCTCC AGTCAGAGCT ACAGATCTAT 
GAGGCACTTT ATGGTGACTC CAAGAAGACG CTGAAAGCTT ATACCTGGGA TGCCTGTCAC 
CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC CTGGTGGCAG AGATCCGAGC TTTGCGAGGG 
CAGCTGGAGC AGAGCATTCA GGTGAACAAC TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG 
GATGGTGGTG CCAGCAAAGC TGGCCTCAGC TCCTCCTCCG TTAACCAGAA ATTCCCCACC 
AACACTGACC CTGGAAACAA GCAGCCATTC TTCCAAGATT CGGTTGCCTC CCCTCCAGTT 
CGGGATGTGG GCATGAATTC TCCAGTTCTC GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT 
GGCCCAGAGA CCACAACCTT CAGTAGGACG AACGAGCTGG GTTTGGATGC TTCTCCAGTA 
ATGAAGAACC CTCGCAAGCT GGAGGGCGAC GCTACTGATG GCTCCTTTGC CAATAAGCAT 
GGCCGCCACG TCATTGGCCA CATTGATGAC TATAGTGCCC TAAGAGAGCA GATTGGGGAG 
GGCAGACTGC TGGTCAAGAA GATAGCATCA CTGGTGAGAC CAACCTGCAG CTTCTCTGGT 
CTTGAAGCTC CAGGCATAGA GCCACAGGGA TGCCCATGGC TACTGCCCCT TTCCCAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318919.1
CDS	345..7589
Translation	MMGRWYPEQVMQQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015463433.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463433.2

ORF Insert Sequence:

ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC ACCTCAATGA CCTGAAGAAG 
GAGAACTTCA GCCTCAAGCT GCGCATCTAC TTCCTGGAGG AGCGCATGCA GCAGAAGTAT 
GAGGCCAGCC GGGATGACAT CTACAAGCGG AACATCGAGC TGAAGGTTGA AGTAGAGAGC 
CTGAAACGAG AACTCCAGGA CAAGAGACAG CATCCGGATA AAACATGCGC TGATGCAGAG 
AATATCAACA GCCATAATGA AGCTGAGCTC CGGCGCCAGT TTGAGGAGAG ACAGCAGGAG 
ACGGAGCATG TTTACGAGCT CCTGGAGAAT AAGATCCAGC TTCTGCAAGA GGAGTCCAGA 
CTAGCAAAAA ATGAAGCCGC ACGGATGGCG GCTCTGGTGG AAGCAGAGAA AGAGTGTAAC 
CTGGAGCTCT CAGAGAAACT GAAGGGAGTC ACCAAGGAGA GGGAAGATGT ACCAGGAGAC 
CGGGTCAAAT CTGACCAGTA CACTGAGGCC CTGGCCCAGA GGGACAAAAA AATTGAAGAA 
CTGAGTCAAA GCCTGGCTGC ACAGGAGAAA CTTGTAGAGC AGCTGTCTCA AGAGAAACAA 
CAACTGCTAC ATCTGTTGGA GGAGCCAGCT GACATGGAAG TGCAGCCCAT GACTGAAGAC 
TTACTCAAAC AACAGAAGTT GAATTCAAAT GAGACCGGTA CAACTCAGCA GTCTGTATCT 
GATTCCCATC TGGCAGAACT CCAGGAAAAA ATCCAGCAAA CAGAGACCAC CAACAAGATG 
CTTCAAGAAA AACTGAATGA AATGAGCTAT GAACTGAAGT CTGCTCAGGA GTCATCTCAG 
AAGCAAGATG ATACAATTCA AAACCTCAAG GAAACTCTGA AAAGCAGGGA AAGTGAGACC 
GAGGAGTTGT ACCAGGTGAT TGAAGGTCAA AACGACACAA TGGCTAAGCT TCGAGAAATG 
CTGCACCAGA GTCAGCTTAA ACAGCTTCAT AACTCAGAGG GTACTTCCCC AGCTCAGCAA 
CAGGTGGCTC TGCTTGATCT TCAAAGTGCT TTATTCTGCA GCCAGCTTGA AATACAGAAG 
CTCCAGAGGG CAGTACGCCA AAAAGAAAGG CAGCTGGCTG ATGCTAGACG ATGTGTGCGG 
TTTGTAGAGG CTGCAGCACA GGAGAGAGAA CAGCAGAAAG AGGCTTCTTG GAAACATAAC 
CAAGAATTGC AAAAAGCCCT ACAGCAGCTA CAAGGAGAAC TTCAGAGTAA GAGCCAACAG 
CTTCATACCC TGGAGGCTGA AAAATATAAT GAGATTCGCA CCCAAGAGCA GCACATTCAG 
CACCTCCACT ACAGTCTGAG TCACAAAGAG CAGCTGCTTC AGGAATTTCG GGAGCTCCTG 
CAGTACCGAA ATAACTCAGA CAAAACCCTT GAAGCAAATG AAATGTTGCT TGAGAAACTT 
AGGCAAAGAA TACAAGATAG AGATGTTGCC CTAGAGCGGG CTATAGATGA AAAGTTCTCC 
AGCCTAGAAG AAAAGGAAAA AGAACTGCGT CAGCTTCACC TTGCCATGAG AGAGCGAGAT 
CACGACTTAG AGAGGCTGCG CGGTATCCTC TCCGCCAATG AAGCTACCAT GCAGAGTATG 
GAGAGTCTCC TGAGGTCCAA AGGCCTGGAA GTGGAACAGT TGTCTGCCAC CTGTCAGAAC 
CTCCAGTGGC TGAAAGAAGA AATGGAAACC AAATTTAGCC GTTGGCAGAA GGAGCAAGAA 
AGCATCATTC AGCAGCTACA GACATCTCTG CATGACAGGA ACAAAGAAGT GGAGGATCTT 
ACCGCAACAT TGCTCTGCAA ACTTGGACCA GGGCAGACTG AGATAGTTGA GGAGCTGTGC 
CAGCGTCTAC AGCAGAAGGA AAGGATGCTG CAGGACCTTC TGAGTGATCG GAACAAGCAA 
GCAGTGGAAT ATGAAATGGA GATTCAGGGC CTGCTTCAGT CCATGAGCAC CAGAGAGCAG 
GAGAGCCACG CTGCTGCAGA AAAGATGGTA CAAGCTCTAA TGGAAAGAAA TTCAGAATTA 
CAGGCCCTGC GCCAATATTT AGGAGGGAAA GATTTCATGA TGTCCCAAGC ACCCCTCTCT 
AACCGACCAG CTGAAGTTAC CTCCATCAGC CCCCATCTTG GAGAGCAAAC TGATCAAGGT 
TCAGTGCATA TACCCTCCAG AGATGATAGC ACTTCATTGA CTGCCAAAGG GGATGCTAGC 
GTACCCAGGT CCTCGTTAGG GGAGGTGGAT ACAGTTGCGG GGCTGGAAAA AGAACTGAGT 
AATGCCAAAG AGGAACTTGA GCTCATGGCT AAAAAAGAGA GAGAAAGTCG GATGGAACTG 
TCTGCACTCC AGTCCATGGT GGCTGTGCAG GAGCAAGAGC TGCAGGTTCA GGCTGCTGAC 
ATGGAGTCCC TGACCAGGAC CATACAGATT AAGGAAGACC TCATAAAGGA CCTGCAGATG 
CAGCTGGTTG ATCCTGAAGA CATCCCAGCT ATGGAACGCC TGACCCAGGA AGTCTTGCTT 
CTTCGGGAAA AAGTGGCTTC AGTAGAATCA CAAGGACAGG AAACGTCTGG AAACCGAAGA 
CAACAATTGC TGCTGATGCT CGAAGGACTG GTAGATGAGC GGAGTCGGCT CAATGAGGCC 
TTACAGGCAG AGAGACAGCT CTATAGCAGT CTGGTGAAGT TCCATGCCCA TCCAGAGAGC 
TCTGAGAGAG ACCGAACTCT GCAGGTGGAA CTAGAAGGGG CCCAGGTGAT ACGCAATCGG 
CTAGAAGAAG TTCTCGGAAG AAGCCTGGAG CGCTTAAGCA GGCTGGAGAC CCTGGCCGCC 
ATTGGAGGTA CAGCTGCAGG GGACGACACG GAGGATGCGA GCACGGAGTT TACCGACAGT 
ATCGAGGAGG AGGCTGCACA CAACAGCCAC CAGCAACTCA TCAAGGTGGC GTTGGAGAAA 
AGCCGGGCAG CTGTGGAGAC CCAGAATGTG TCTCCTGCTC CTCCCACCCT GACAGGAGGG 
GACGGTAACA GGGGTCTTCA GGAGGAAATG CTCCACCTGA GGGCTGAAAT TCACCGGCAC 
TTAGAGGAGA AGAGGAAAGC TGAGGGGGAA CTGAAGGAGC TAAAGGCTCA AATTGAGGAA 
GCAGGATTCT CCTCTGTGGC CCACATCAGG AACACCATGC TGAGCCTTTG CCTTGAGAAT 
GCAGAGCTGA AAGAGCAGAT GGGAGAAGCA ATGTCTGATG GATGGGAGAT TGAGGAAGAC 
AAGGAGAAGG GCGAGGCGAT GGTGGAGACT GTGGTTGCCA AAGGGGTTCT GAATGAGAAT 
AGCCTTCAGG CTGAATTCAG AAAGGTCCAG GGAAAACTGA AGAATGCCCG CAATATCATC 
AGCCTCCTCA AAGAACAGCT GGTGTTGAGC AGCAAGGAAG GGACTAGTAA GCTTAATCCT 
GAGCTCCTTG TGCACCTGGC CAAGGAGATC AACAGAATGA ACACAGAGCT GGTTTACTCT 
CCTGGAAAGC ACCAATGCCT AGAAGAGGAG GGTGTGACCA CAAGGCCGTG CCCCAGACCC 
CAGAGCCTTG ACCTTGGGGC TGCCCTCACA GTGGATGCTC ACCAACTGGA TAACCAGCCC 
CAGACCCATG ACCCTGGGCC GCAGTCAGAA TTTAGCTTCT CAGGATCCAC GAAGCACCTG 
CGCTCCCAGT TGGCACAATG CAAACAACGC TATCAGGATC TCCAGGAGAA GCTGCTGATA 
TCAGAAGCCA CTGTCTTTGC CCAGGCCAAC CAGCTGGAGA AATACAGAGT CATGTTTACA 
GGTGGATCGT CGGTGAAGCA GGACAGCAAG CAGGTGCAGG TGGACCTCCA GGACCTGGGC 
TATGAGACCT GTGGCCGAAG CGAGAACGAG GCTGAGCGGG AGGAGAGCAC CAGCCCTGAG 
TGTGAGGAGC ACGACAGCCT CAGGGAAACA GTCTTGATGG AGGGGCTGTG CTCCAAGCAG 
GGACACCTGG ACTCCACAAT GACCAGCCCC CCTGGGAAGA AGCCCCTGGA GAGCCAACCA 
GGGAAGCAGG AGGAGCTCCG GGCGTATGGA AAGTCAGAAG ACATCTCTGT CCTACGTAAG 
GACATCAAAG ATCTGAAGGC CCAGCTGCAG AACGCCAACA AGGTCATTCA GAACCTGAAG 
AGCCGGGTTC GGTCCCTCTC AGTTACAAGC GATTATTCAT CTAGTCTGGA GAGACCCCGA 
AAGCTGAAGG CCGTTGGTGC CCTAGAAGGA ACTTCACCGC ACAGTGTCAC TGATGAGGAC 
GAGGGGTGGC TGTCTGATGG CACTGGGGCT TTCTACCCTC CAGGGCTTCA GGCTAAAAAG 
GATCTGGAGA GTCTGATCCA GAGGGTGTCC CAGCTAGAAG CCCAGCTCCC AAAAACTGGA 
ACGGAAGGGA AGCTGGCCGA GGAGCTGAGA TCTGCTTCGT GGCCTGGGAA ATATGATTCC 
CTGATTCAGG ACCAGGCCCG AGAACTGTCC TACCTACGTC AGAAAATACG GGAAGGGAGA 
GGTATCTGTT ATCTTCTCAC CCAGCACTCG AAAGACACAG TAAAGTCTTT CGAGGACCTC 
CTAAGGAGCA ATGACATTGA CTACTACCTG GGACAGAGCT TCCGGGAGCA CCTCGCCCAG 
GGCAGCCAGC TGACAGAAAG GCTCACCAGC AAACTCAGCA CCAAGGATCA TAAAAGTGAG 
AAAGATCAAG CTGGACTCGA GCCACTGGCT CTGAGGCTTA GCAGGGAGCT GCAGGAGAAG 
GAGAAAGTGA TCGAAGTCCT GCAGGCCAAG CTGGACGCCC GCTCCCTCAC ACCGTGCAGC 
AGCCATGCCT TGTCAGACTC TCAGCGCTCT CCCAGCAGCT CCTCCTTCTT GTCTGACGAG 
CTGGAAGCCT GCTCTGACAT GGACATCGCC AGCGAGTACA CACACTATGA AGAGAAGAAG 
GCTTCACCTG GTCACTCAGA TTCCATCCAT CATTCGAGTC ATTCTGCTGT ACTGTCTTCC 
AAACCATCAG CAACCAGTTC ATCTCAGGAG GTTAAGGCCG AACCCAGCAG CAACCCCGTC 
AGCTTCCCAG CTCCCCAGAA TCCCCCCAAG GAGGCCAGCC AGGCCCATCC AGGCCTTCAT 
TTCCAATCCA TACCCACGCT GGTTAGCCTT CCCCAGGCAC CACTGCCCTC AGCTCCACCC 
AGCTTCCTGC CTTTCAGCTC TGCTGGCCCT CCCCTCCCTG GCTGCTGTGA GACACCCATG 
GTCTCCTTGG CAGAGGCTCA GCAGGAGCTG CAGATGCTGC GGAAGCAGTT AGGAGAAAGT 
GTCACCAGTG TTCATCCTGC TTCCCCAGCC ACACTGCCAA CCAACCACAC AGAAGCCAGA 
TCTTCCCACT ACCTCAACCC TCCCCAGCCC CTCTGTCCTC CAAGGGGCAC CACGGACCTG 
GGCAGAATCC TGGAGCCTGG GTACCTGGGT AGCAGTGGCC AGTGGGACGT GATGAGGCCT 
CAGAAAGGGA GCATATCCGG GGACCTATCC TCAGGCTCCT CCATGTACCA GCTTAACTCC 
AAGCCCACAG GGGCTGACCT GCTAGAGGAG CATCTTGGTG AAATCCGGAA CCTACGCCAG 
CGCCTGGAGG AGTCCATCTG CATTAATGAC CGCCTGCGGG AGCAGCTGGA AAACAGGCTC 
AGCTCCACTG CCCGCGAAAG TGGCTCAACC TCTAACTTCT ACAGTCCAGG CCTGGAGTCC 
ACACCTCAGC TCTCCAATGA GAACAGAGTC CTTAGGGAAG AAAACCACAG GCTTCAGGCT 
CAGCTCAGTC ATGTTTCCAG AGAGCACTCC CAGGAAACAG AATGCCTGAG GGAGGCTCTG 
CTGACCTCTC GATCCCGGCT TCAAGAGCTG GAAATGGAGC TGGAGCACCA AAAGGTAGAC 
CGGCAGCAGT TACTGGAAGA CCTGAAGGAG AAGCAGCAGG AGATCTTGCA TTTCCAGGAG 
GAACGACTGT CTCTCCAGGA GAAAGACTCC AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG 
CAACAGTGCG AAGAGAAACA GCAGCTCTTC CAGTCCCTCC AGTCAGAGCT ACAGATCTAT 
GAGGCACTTT ATGGTGACTC CAAGAAGACG CTGAAAGCTT ATACCTGGGA TGCCTGTCAC 
CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC CTGGTGGCAG AGATCCGAGC TTTGCGAGGG 
CAGCTGGAGC AGAGCATTCA GGTGAACAAC TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG 
GATGGTGGTG CCAGCAAAGC TGGCCTCAGC TCCTCCTCCG TTAACCAGAA ATTCCCCACC 
AACACTGACC CTGGAAACAA GCAGCCATTC TTCCAAGATT CGGTTGCCTC CCCTCCAGTT 
CGGGATGTGG GCATGAATTC TCCAGTTCTC GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT 
GGCCCAGAGA CCACAACCTT CAGTAGGACG AACGAGCTGG GTTTGGATGC TTCTCCAGTA 
ATGAAGAACC CTCGCAAGCT GGAGGGCGAC GCTACTGATG GCTCCTTTGC CAATAAGCAT 
GGCCGCCACG TCATTGGCCA CATTGATGAC TATAGTGCCC TAAGAGAGCA GATTGGGGAG 
GGCAGACTGC TGGTCAAGAA GATAGCATCA CTGGTGAGAC CAACCTGCAG CTTCTCTGGT 
CTTGAAGCTC CAGGCATAGA GCCACAGGGA TGCCCATGGC TACTGCCCCT TTCCCAGGTG 
ATGGGCAGCG AAGGCATCCA GGAGCTGAGG AGCAGCGCCA CCGCCCTGCA CCACCGGCTG 
GAGGAGTCGG CCTCCCTCCT CACCATGTTC TGGCGAGCAG CCTTGCCAAG CTCCCCCGGC 
CCTGTGCTTG TGGACAAAGT GGGAGAGTCT ATGAAAAAGG AGCTTCTGGA ACTGAGAACC 
AAACTATCCA AACAGGAAAG TCTCCTTCAG AGTACATCTG AGCGTCTGAA GACCGCCAAC 
CAGCAGAAGG AGAGCATGGA GCAGTTCATC TTCAGCCAGT TGACCAGGAC ACACGACGTT 
TTGAAGAAGG CAAGGACTAA TTTGGAGAAG ACCTCTTACA AGACTGCTTC TGTAAAGCCT 
TATTCCTGTC CCAGCAAAGG TGAAATCCCT AAGGGCCCTG CCATGCACTC CAGTCCTGTG 
ACCCTTGCCT TCCAGGAGAC ATGCAAGAAG CGAAGCCACC AGAGGTCCTT AAAACAGCAG 
GAAGGATGGG CCTGTGCCCC TTCTTCACAG CTCCCTGTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197743.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197800.1 Bos taurus myomegalin (LOC508547), transcript variant X1, mRNA.

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CloneID	OBa03348
Clone ID Related Accession (Same CDS sequence)	XM_005197743.3 , XM_005204049.3 , XM_005204049.4
Accession Version	XM_005197743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197743.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGAAGACC TCTTACAAGA CTGCTTCTGT AAAGCCTTAT 
TCCTGTCCCA GCAAAGGTGA AATCCCTAAG GGCCCTGCCA TGCACTCCAG TCCTGTGACC 
CTTGCCTTCC AGGAGACATG CAAGAAGCGA AGCCACCAGA GGTCCTTAAA ACAGCAGGAA 
GGATGGGCCT GTGCCCCTTC TTCACAGCTC CCTGTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197800.1
CDS	432..8153
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005197743.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197743.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGAAGACC TCTTACAAGA CTGCTTCTGT AAAGCCTTAT 
TCCTGTCCCA GCAAAGGTGA AATCCCTAAG GGCCCTGCCA TGCACTCCAG TCCTGTGACC 
CTTGCCTTCC AGGAGACATG CAAGAAGCGA AGCCACCAGA GGTCCTTAAA ACAGCAGGAA 
GGATGGGCCT GTGCCCCTTC TTCACAGCTC CCTGTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204049.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204106.1 Bos taurus myomegalin (LOC508547), transcript variant X1, mRNA.

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CloneID	OBa03348
Clone ID Related Accession (Same CDS sequence)	XM_005197743.3 , XM_005204049.3 , XM_005204049.4
Accession Version	XM_005204049.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGAAGACC TCTTACAAGA CTGCTTCTGT AAAGCCTTAT 
TCCTGTCCCA GCAAAGGTGA AATCCCTAAG GGCCCTGCCA TGCACTCCAG TCCTGTGACC 
CTTGCCTTCC AGGAGACATG CAAGAAGCGA AGCCACCAGA GGTCCTTAAA ACAGCAGGAA 
GGATGGGCCT GTGCCCCTTC TTCACAGCTC CCTGTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204106.1
CDS	432..8153
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204049.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204049.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGAAGACC TCTTACAAGA CTGCTTCTGT AAAGCCTTAT 
TCCTGTCCCA GCAAAGGTGA AATCCCTAAG GGCCCTGCCA TGCACTCCAG TCCTGTGACC 
CTTGCCTTCC AGGAGACATG CAAGAAGCGA AGCCACCAGA GGTCCTTAAA ACAGCAGGAA 
GGATGGGCCT GTGCCCCTTC TTCACAGCTC CCTGTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204049.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204106.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X1, mRNA.

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CloneID	OBa03348
Clone ID Related Accession (Same CDS sequence)	XM_005197743.3 , XM_005204049.3 , XM_005204049.4
Accession Version	XM_005204049.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204049.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGAAGACC TCTTACAAGA CTGCTTCTGT AAAGCCTTAT 
TCCTGTCCCA GCAAAGGTGA AATCCCTAAG GGCCCTGCCA TGCACTCCAG TCCTGTGACC 
CTTGCCTTCC AGGAGACATG CAAGAAGCGA AGCCACCAGA GGTCCTTAAA ACAGCAGGAA 
GGATGGGCCT GTGCCCCTTC TTCACAGCTC CCTGTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204106.1
CDS	714..8435
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204049.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204049.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGAAGACC TCTTACAAGA CTGCTTCTGT AAAGCCTTAT 
TCCTGTCCCA GCAAAGGTGA AATCCCTAAG GGCCCTGCCA TGCACTCCAG TCCTGTGACC 
CTTGCCTTCC AGGAGACATG CAAGAAGCGA AGCCACCAGA GGTCCTTAAA ACAGCAGGAA 
GGATGGGCCT GTGCCCCTTC TTCACAGCTC CCTGTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197744.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197801.1 Bos taurus myomegalin (LOC508547), transcript variant X3, mRNA.

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CloneID	OBa03349
Clone ID Related Accession (Same CDS sequence)	XM_005197744.3 , XM_005204050.3 , XM_005204050.4
Accession Version	XM_005197744.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197801.1
CDS	433..8151
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005197744.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197744.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204050.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204107.1 Bos taurus myomegalin (LOC508547), transcript variant X3, mRNA.

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CloneID	OBa03349
Clone ID Related Accession (Same CDS sequence)	XM_005197744.3 , XM_005204050.3 , XM_005204050.4
Accession Version	XM_005204050.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204107.1
CDS	433..8151
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204050.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204050.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204050.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204107.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X3, mRNA.

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CloneID	OBa03349
Clone ID Related Accession (Same CDS sequence)	XM_005197744.3 , XM_005204050.3 , XM_005204050.4
Accession Version	XM_005204050.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204050.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204107.1
CDS	714..8432
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204050.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204050.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204051.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204108.1 Bos taurus myomegalin (LOC508547), transcript variant X2, mRNA.

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CloneID	OBa03350
Clone ID Related Accession (Same CDS sequence)	XM_005204051.3 , XM_005197745.3 , XM_005204051.4
Accession Version	XM_005204051.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204108.1
CDS	432..8150
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204051.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204051.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197745.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197802.1 Bos taurus myomegalin (LOC508547), transcript variant X2, mRNA.

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CloneID	OBa03350
Clone ID Related Accession (Same CDS sequence)	XM_005204051.3 , XM_005197745.3 , XM_005204051.4
Accession Version	XM_005197745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197802.1
CDS	432..8150
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005197745.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197745.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204051.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204108.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X2, mRNA.

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CloneID	OBa03350
Clone ID Related Accession (Same CDS sequence)	XM_005204051.3 , XM_005197745.3 , XM_005204051.4
Accession Version	XM_005204051.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204051.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204108.1
CDS	714..8432
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204051.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204051.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTAGTGGA 
TCGTCGGTGA AGCAGGACAG CAAGCAGGTG CAGGTGGACC TCCAGGACCT GGGCTATGAG 
ACCTGTGGCC GAAGCGAGAA CGAGGCTGAG CGGGAGGAGA GCACCAGCCC TGAGTGTGAG 
GAGCACGACA GCCTCAGGGA AACAGTCTTG ATGGAGGGGC TGTGCTCCAA GCAGGGACAC 
CTGGACTCCA CAATGACCAG CCCCCCTGGG AAGAAGCCCC TGGAGAGCCA ACCAGGGAAG 
CAGGAGGAGC TCCGGGCGTA TGGAAAGTCA GAAGACATCT CTGTCCTACG TAAGGACATC 
AAAGATCTGA AGGCCCAGCT GCAGAACGCC AACAAGGTCA TTCAGAACCT GAAGAGCCGG 
GTTCGGTCCC TCTCAGTTAC AAGCGATTAT TCATCTAGTC TGGAGAGACC CCGAAAGCTG 
AAGGCCGTTG GTGCCCTAGA AGGAACTTCA CCGCACAGTG TCACTGATGA GGACGAGGGG 
TGGCTGTCTG ATGGCACTGG GGCTTTCTAC CCTCCAGGGC TTCAGGCTAA AAAGGATCTG 
GAGAGTCTGA TCCAGAGGGT GTCCCAGCTA GAAGCCCAGC TCCCAAAAAC TGGAACGGAA 
GGGAAGCTGG CCGAGGAGCT GAGATCTGCT TCGTGGCCTG GGAAATATGA TTCCCTGATT 
CAGGACCAGG CCCGAGAACT GTCCTACCTA CGTCAGAAAA TACGGGAAGG GAGAGGTATC 
TGTTATCTTC TCACCCAGCA CTCGAAAGAC ACAGTAAAGT CTTTCGAGGA CCTCCTAAGG 
AGCAATGACA TTGACTACTA CCTGGGACAG AGCTTCCGGG AGCACCTCGC CCAGGGCAGC 
CAGCTGACAG AAAGGCTCAC CAGCAAACTC AGCACCAAGG ATCATAAAAG TGAGAAAGAT 
CAAGCTGGAC TCGAGCCACT GGCTCTGAGG CTTAGCAGGG AGCTGCAGGA GAAGGAGAAA 
GTGATCGAAG TCCTGCAGGC CAAGCTGGAC GCCCGCTCCC TCACACCGTG CAGCAGCCAT 
GCCTTGTCAG ACTCTCAGCG CTCTCCCAGC AGCTCCTCCT TCTTGTCTGA CGAGCTGGAA 
GCCTGCTCTG ACATGGACAT CGCCAGCGAG TACACACACT ATGAAGAGAA GAAGGCTTCA 
CCTGGTCACT CAGATTCCAT CCATCATTCG AGTCATTCTG CTGTACTGTC TTCCAAACCA 
TCAGCAACCA GTTCATCTCA GGAGGTTAAG GCCGAACCCA GCAGCAACCC CGTCAGCTTC 
CCAGCTCCCC AGAATCCCCC CAAGGAGGCC AGCCAGGCCC ATCCAGGCCT TCATTTCCAA 
TCCATACCCA CGCTGGTTAG CCTTCCCCAG GCACCACTGC CCTCAGCTCC ACCCAGCTTC 
CTGCCTTTCA GCTCTGCTGG CCCTCCCCTC CCTGGCTGCT GTGAGACACC CATGGTCTCC 
TTGGCAGAGG CTCAGCAGGA GCTGCAGATG CTGCGGAAGC AGTTAGGAGA AAGTGTCACC 
AGTGTTCATC CTGCTTCCCC AGCCACACTG CCAACCAACC ACACAGAAGC CAGATCTTCC 
CACTACCTCA ACCCTCCCCA GCCCCTCTGT CCTCCAAGGG GCACCACGGA CCTGGGCAGA 
ATCCTGGAGC CTGGGTACCT GGGTAGCAGT GGCCAGTGGG ACGTGATGAG GCCTCAGAAA 
GGGAGCATAT CCGGGGACCT ATCCTCAGGC TCCTCCATGT ACCAGCTTAA CTCCAAGCCC 
ACAGGGGCTG ACCTGCTAGA GGAGCATCTT GGTGAAATCC GGAACCTACG CCAGCGCCTG 
GAGGAGTCCA TCTGCATTAA TGACCGCCTG CGGGAGCAGC TGGAAAACAG GCTCAGCTCC 
ACTGCCCGCG AAAGTGGCTC AACCTCTAAC TTCTACAGTC CAGGCCTGGA GTCCACACCT 
CAGCTCTCCA ATGAGAACAG AGTCCTTAGG GAAGAAAACC ACAGGCTTCA GGCTCAGCTC 
AGTCATGTTT CCAGAGAGCA CTCCCAGGAA ACAGAATGCC TGAGGGAGGC TCTGCTGACC 
TCTCGATCCC GGCTTCAAGA GCTGGAAATG GAGCTGGAGC ACCAAAAGGT AGACCGGCAG 
CAGTTACTGG AAGACCTGAA GGAGAAGCAG CAGGAGATCT TGCATTTCCA GGAGGAACGA 
CTGTCTCTCC AGGAGAAAGA CTCCAGGCTG CAGCGTAAGC TGGCCCTCCT GCAGCAACAG 
TGCGAAGAGA AACAGCAGCT CTTCCAGTCC CTCCAGTCAG AGCTACAGAT CTATGAGGCA 
CTTTATGGTG ACTCCAAGAA GACGCTGAAA GCTTATACCT GGGATGCCTG TCACCAAGTC 
CCTTTGAGCA GTGACTTGAG CCACCTGGTG GCAGAGATCC GAGCTTTGCG AGGGCAGCTG 
GAGCAGAGCA TTCAGGTGAA CAACTGTCTG CGATTGCAGC TCGAACAGCA GTTGGATGGT 
GGTGCCAGCA AAGCTGGCCT CAGCTCCTCC TCCGTTAACC AGAAATTCCC CACCAACACT 
GACCCTGGAA ACAAGCAGCC ATTCTTCCAA GATTCGGTTG CCTCCCCTCC AGTTCGGGAT 
GTGGGCATGA ATTCTCCAGT TCTCGTCTTC CCCAGCTCTT CTTCTGCGGC TCCTGGCCCA 
GAGACCACAA CCTTCAGTAG GACGAACGAG CTGGGTTTGG ATGCTTCTCC AGTAATGAAG 
AACCCTCGCA AGCTGGAGGG CGACGCTACT GATGGCTCCT TTGCCAATAA GCATGGCCGC 
CACGTCATTG GCCACATTGA TGACTATAGT GCCCTAAGAG AGCAGATTGG GGAGGGCAGA 
CTGCTGGTCA AGAAGATAGC ATCACTGGTG AGACCAACCT GCAGCTTCTC TGGTCTTGAA 
GCTCCAGGCA TAGAGCCACA GGGATGCCCA TGGCTACTGC CCCTTTCCCA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GAAGACCTCT TACAAGACTG CTTCTGTAAA GCCTTATTCC 
TGTCCCAGCA AAGGTGAAAT CCCTAAGGGC CCTGCCATGC ACTCCAGTCC TGTGACCCTT 
GCCTTCCAGG AGACATGCAA GAAGCGAAGC CACCAGAGGT CCTTAAAACA GCAGGAAGGA 
TGGGCCTGTG CCCCTTCTTC ACAGCTCCCT GTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204052.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204109.1 Bos taurus myomegalin (LOC508547), transcript variant X4, mRNA.

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CloneID	OBa03351
Clone ID Related Accession (Same CDS sequence)	XM_005204052.3 , XM_005204052.4 , XM_005197746.3
Accession Version	XM_005204052.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGAA GACCTCTTAC 
AAGACTGCTT CTGTAAAGCC TTATTCCTGT CCCAGCAAAG GTGAAATCCC TAAGGGCCCT 
GCCATGCACT CCAGTCCTGT GACCCTTGCC TTCCAGGAGA CATGCAAGAA GCGAAGCCAC 
CAGAGGTCCT TAAAACAGCA GGAAGGATGG GCCTGTGCCC CTTCTTCACA GCTCCCTGTC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204109.1
CDS	432..8117
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204052.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204052.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGAA GACCTCTTAC 
AAGACTGCTT CTGTAAAGCC TTATTCCTGT CCCAGCAAAG GTGAAATCCC TAAGGGCCCT 
GCCATGCACT CCAGTCCTGT GACCCTTGCC TTCCAGGAGA CATGCAAGAA GCGAAGCCAC 
CAGAGGTCCT TAAAACAGCA GGAAGGATGG GCCTGTGCCC CTTCTTCACA GCTCCCTGTC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204052.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204109.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X4, mRNA.

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CloneID	OBa03351
Clone ID Related Accession (Same CDS sequence)	XM_005204052.3 , XM_005204052.4 , XM_005197746.3
Accession Version	XM_005204052.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204052.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGAA GACCTCTTAC 
AAGACTGCTT CTGTAAAGCC TTATTCCTGT CCCAGCAAAG GTGAAATCCC TAAGGGCCCT 
GCCATGCACT CCAGTCCTGT GACCCTTGCC TTCCAGGAGA CATGCAAGAA GCGAAGCCAC 
CAGAGGTCCT TAAAACAGCA GGAAGGATGG GCCTGTGCCC CTTCTTCACA GCTCCCTGTC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204109.1
CDS	713..8398
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204052.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204052.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGAA GACCTCTTAC 
AAGACTGCTT CTGTAAAGCC TTATTCCTGT CCCAGCAAAG GTGAAATCCC TAAGGGCCCT 
GCCATGCACT CCAGTCCTGT GACCCTTGCC TTCCAGGAGA CATGCAAGAA GCGAAGCCAC 
CAGAGGTCCT TAAAACAGCA GGAAGGATGG GCCTGTGCCC CTTCTTCACA GCTCCCTGTC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197746.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197803.1 Bos taurus myomegalin (LOC508547), transcript variant X4, mRNA.

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CloneID	OBa03351
Clone ID Related Accession (Same CDS sequence)	XM_005204052.3 , XM_005204052.4 , XM_005197746.3
Accession Version	XM_005197746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGAA GACCTCTTAC 
AAGACTGCTT CTGTAAAGCC TTATTCCTGT CCCAGCAAAG GTGAAATCCC TAAGGGCCCT 
GCCATGCACT CCAGTCCTGT GACCCTTGCC TTCCAGGAGA CATGCAAGAA GCGAAGCCAC 
CAGAGGTCCT TAAAACAGCA GGAAGGATGG GCCTGTGCCC CTTCTTCACA GCTCCCTGTC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197803.1
CDS	432..8117
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005197746.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197746.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGAA GACCTCTTAC 
AAGACTGCTT CTGTAAAGCC TTATTCCTGT CCCAGCAAAG GTGAAATCCC TAAGGGCCCT 
GCCATGCACT CCAGTCCTGT GACCCTTGCC TTCCAGGAGA CATGCAAGAA GCGAAGCCAC 
CAGAGGTCCT TAAAACAGCA GGAAGGATGG GCCTGTGCCC CTTCTTCACA GCTCCCTGTC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197748.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197805.1 Bos taurus myomegalin (LOC508547), transcript variant X7, mRNA.

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CloneID	OBa03353
Clone ID Related Accession (Same CDS sequence)	XM_005197748.3 , XM_005204055.3 , XM_005204055.4
Accession Version	XM_005197748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197748.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197805.1
CDS	433..7998
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005197748.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197748.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204055.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204112.1 Bos taurus myomegalin (LOC508547), transcript variant X7, mRNA.

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CloneID	OBa03353
Clone ID Related Accession (Same CDS sequence)	XM_005197748.3 , XM_005204055.3 , XM_005204055.4
Accession Version	XM_005204055.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204112.1
CDS	433..7998
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005204055.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204055.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204055.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204112.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X10, mRNA.

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CloneID	OBa03353
Clone ID Related Accession (Same CDS sequence)	XM_005197748.3 , XM_005204055.3 , XM_005204055.4
Accession Version	XM_005204055.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204055.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204112.1
CDS	714..8279
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005204055.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204055.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGCC ACAGGGATGC CCATGGCTAC TGCCCCTTTC CCAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197749.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197806.1 Bos taurus myomegalin (LOC508547), transcript variant X10, mRNA.

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CloneID	OBa03354
Clone ID Related Accession (Same CDS sequence)	XM_005197749.3 , XM_005204056.3 , XM_005204056.4
Accession Version	XM_005197749.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGGT GAAATCCCTA 
AGGGCCCTGC CATGCACTCC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197806.1
CDS	433..7962
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005197749.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197749.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGGT GAAATCCCTA 
AGGGCCCTGC CATGCACTCC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204056.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204113.1 Bos taurus myomegalin (LOC508547), transcript variant X10, mRNA.

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CloneID	OBa03354
Clone ID Related Accession (Same CDS sequence)	XM_005197749.3 , XM_005204056.3 , XM_005204056.4
Accession Version	XM_005204056.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGGT GAAATCCCTA 
AGGGCCCTGC CATGCACTCC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204113.1
CDS	433..7962
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005204056.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204056.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGGT GAAATCCCTA 
AGGGCCCTGC CATGCACTCC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204056.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204113.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X15, mRNA.

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CloneID	OBa03354
Clone ID Related Accession (Same CDS sequence)	XM_005197749.3 , XM_005204056.3 , XM_005204056.4
Accession Version	XM_005204056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204056.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGGT GAAATCCCTA 
AGGGCCCTGC CATGCACTCC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204113.1
CDS	714..8243
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005204056.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204056.4

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG CCTTCATTTC 
CAATCCATAC CCACGCTGGT TAGCCTTCCC CAGGCACCAC TGCCCTCAGC TCCACCCAGC 
TTCCTGCCTT TCAGCTCTGC TGGCCCTCCC CTCCCTGGCT GCTGTGAGAC ACCCATGGTC 
TCCTTGGCAG AGGCTCAGCA GGAGCTGCAG ATGCTGCGGA AGCAGTTAGG AGAAAGTGTC 
ACCAGTGTTC ATCCTGCTTC CCCAGCCACA CTGCCAACCA ACCACACAGA AGCCAGATCT 
TCCCACTACC TCAACCCTCC CCAGCCCCTC TGTCCTCCAA GGGGCACCAC GGACCTGGGC 
AGAATCCTGG AGCCTGGGTA CCTGGGTAGC AGTGGCCAGT GGGACGTGAT GAGGCCTCAG 
AAAGGGAGCA TATCCGGGGA CCTATCCTCA GGCTCCTCCA TGTACCAGCT TAACTCCAAG 
CCCACAGGGG CTGACCTGCT AGAGGAGCAT CTTGGTGAAA TCCGGAACCT ACGCCAGCGC 
CTGGAGGAGT CCATCTGCAT TAATGACCGC CTGCGGGAGC AGCTGGAAAA CAGGCTCAGC 
TCCACTGCCC GCGAAAGTGG CTCAACCTCT AACTTCTACA GTCCAGGCCT GGAGTCCACA 
CCTCAGCTCT CCAATGAGAA CAGAGTCCTT AGGGAAGAAA ACCACAGGCT TCAGGCTCAG 
CTCAGTCATG TTTCCAGAGA GCACTCCCAG GAAACAGAAT GCCTGAGGGA GGCTCTGCTG 
ACCTCTCGAT CCCGGCTTCA AGAGCTGGAA ATGGAGCTGG AGCACCAAAA GGTAGACCGG 
CAGCAGTTAC TGGAAGACCT GAAGGAGAAG CAGCAGGAGA TCTTGCATTT CCAGGAGGAA 
CGACTGTCTC TCCAGGAGAA AGACTCCAGG CTGCAGCGTA AGCTGGCCCT CCTGCAGCAA 
CAGTGCGAAG AGAAACAGCA GCTCTTCCAG TCCCTCCAGT CAGAGCTACA GATCTATGAG 
GCACTTTATG GTGACTCCAA GAAGACGCTG AAAGCTTATA CCTGGGATGC CTGTCACCAA 
GTCCCTTTGA GCAGTGACTT GAGCCACCTG GTGGCAGAGA TCCGAGCTTT GCGAGGGCAG 
CTGGAGCAGA GCATTCAGGT GAACAACTGT CTGCGATTGC AGCTCGAACA GCAGTTGGAT 
GGTGGTGCCA GCAAAGCTGG CCTCAGCTCC TCCTCCGTTA ACCAGAAATT CCCCACCAAC 
ACTGACCCTG GAAACAAGCA GCCATTCTTC CAAGATTCGG TTGCCTCCCC TCCAGTTCGG 
GATGTGGGCA TGAATTCTCC AGTTCTCGTC TTCCCCAGCT CTTCTTCTGC GGCTCCTGGC 
CCAGAGACCA CAACCTTCAG TAGGACGAAC GAGCTGGGTT TGGATGCTTC TCCAGTAATG 
AAGAACCCTC GCAAGCTGGA GGGCGACGCT ACTGATGGCT CCTTTGCCAA TAAGCATGGC 
CGCCACGTCA TTGGCCACAT TGATGACTAT AGTGCCCTAA GAGAGCAGAT TGGGGAGGGC 
AGACTGCTGG TCAAGAAGAT AGCATCACTG GTGAGACCAA CCTGCAGCTT CTCTGGTCTT 
GAAGCTCCAG GCATAGAGGT GATGGGCAGC GAAGGCATCC AGGAGCTGAG GAGCAGCGCC 
ACCGCCCTGC ACCACCGGCT GGAGGAGTCG GCCTCCCTCC TCACCATGTT CTGGCGAGCA 
GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT GTGGACAAAG TGGGAGAGTC TATGAAAAAG 
GAGCTTCTGG AACTGAGAAC CAAACTATCC AAACAGGAAA GTCTCCTTCA GAGTACATCT 
GAGCGTCTGA AGACCGCCAA CCAGCAGAAG GAGAGCATGG AGCAGTTCAT CTTCAGCCAG 
TTGACCAGGA CACACGACGT TTTGAAGAAG GCAAGGACTA ATTTGGAGGT GAAATCCCTA 
AGGGCCCTGC CATGCACTCC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803127.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801429.1 Bos taurus myomegalin (LOC508547), transcript variant X17, mRNA.

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CloneID	OBa03355
Clone ID Related Accession (Same CDS sequence)	XM_010803127.2 , XM_005197750.3
Accession Version	XM_010803127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801429.1
CDS	171..7406
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010803127.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803127.2

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197750.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197807.1 Bos taurus myomegalin (LOC508547), transcript variant X17, mRNA.

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CloneID	OBa03355
Clone ID Related Accession (Same CDS sequence)	XM_010803127.2 , XM_005197750.3
Accession Version	XM_005197750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197807.1
CDS	171..7406
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005197750.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197750.3

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204061.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204118.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X26, mRNA.

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CloneID	OBa03357
Clone ID Related Accession (Same CDS sequence)	XM_005204061.4 , XM_005197752.3 , XM_005204061.3
Accession Version	XM_005204061.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204061.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204118.1
CDS	220..7263
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005204061.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204061.4

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005197752.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197809.1 Bos taurus myomegalin (LOC508547), transcript variant X19, mRNA.

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CloneID	OBa03357
Clone ID Related Accession (Same CDS sequence)	XM_005204061.4 , XM_005197752.3 , XM_005204061.3
Accession Version	XM_005197752.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197809.1
CDS	171..7214
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005197752.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197752.3

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204061.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204118.1 Bos taurus myomegalin (LOC508547), transcript variant X19, mRNA.

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CloneID	OBa03357
Clone ID Related Accession (Same CDS sequence)	XM_005204061.4 , XM_005197752.3 , XM_005204061.3
Accession Version	XM_005204061.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204118.1
CDS	171..7214
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_005204061.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204061.3

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204058.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204115.1 Bos taurus myomegalin (LOC508547), transcript variant X13, mRNA.

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CloneID	OBa17544
Clone ID Related Accession (Same CDS sequence)	XM_005204058.3 , XM_010826853.2 , XM_005204058.4
Accession Version	XM_005204058.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CGTGGACACG AGATTATTTT GCAGAAGATG ATGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACCAAAGATC GAGGCCCTCC TCTGCAGTCA 
CAGACTTGGA GAAGTGGTGA CAAGATCCCC TTGGGGCATT CCTTGAGAGC ATTTGAAAGA 
CCTCTTCAGG TGCAAACCCA GGCCCTTAGA GACTTTGAGA AGCACCTCAA TGACCTGAAG 
AAGGAGAACT TCAGCCTCAA GCTGCGCATC TACTTCCTGG AGGAGCGCAT GCAGCAGAAG 
TATGAGGCCA GCCGGGATGA CATCTACAAG CGGAACATCG AGCTGAAGGT TGAAGTAGAG 
AGCCTGAAAC GAGAACTCCA GGACAAGAGA CAGCATCCGG ATAAAACATG CGCTGATGCA 
GAGAATATCA ACAGCCATAA TGAAGCTGAG CTCCGGCGCC AGTTTGAGGA GAGACAGCAG 
GAGACGGAGC ATGTTTACGA GCTCCTGGAG AATAAGATCC AGCTTCTGCA AGAGGAGTCC 
AGACTAGCAA AAAATGAAGC CGCACGGATG GCGGCTCTGG TGGAAGCAGA GAAAGAGTGT 
AACCTGGAGC TCTCAGAGAA ACTGAAGGGA GTCACCAAGG AGAGGGAAGA TGTACCAGGA 
GACCGGGTCA AATCTGACCA GTACACTGAG GCCCTGGCCC AGAGGGACAA AAAAATTGAA 
GAACTGAGTC AAAGCCTGGC TGCACAGGAG AAACTTGTAG AGCAGCTGTC TCAAGAGAAA 
CAACAACTGC TACATCTGTT GGAGGAGCCA GCTGACATGG AAGTGCAGCC CATGACTGAA 
GACTTACTCA AACAACAGAA GTTGAATTCA AATGAGACCG GTACAACTCA GCAGTCTGTA 
TCTGATTCCC ATCTGGCAGA ACTCCAGGAA AAAATCCAGC AAACAGAGAC CACCAACAAG 
ATGCTTCAAG AAAAACTGAA TGAAATGAGC TATGAACTGA AGTCTGCTCA GGAGTCATCT 
CAGAAGCAAG ATGATACAAT TCAAAACCTC AAGGAAACTC TGAAAAGCAG GGAAAGTGAG 
ACCGAGGAGT TGTACCAGGT GATTGAAGGT CAAAACGACA CAATGGCTAA GCTTCGAGAA 
ATGCTGCACC AGAGTCAGCT TAAACAGCTT CATAACTCAG AGGGTACTTC CCCAGCTCAG 
CAACAGGTGG CTCTGCTTGA TCTTCAAAGT GCTTTATTCT GCAGCCAGCT TGAAATACAG 
AAGCTCCAGA GGGCAGTACG CCAAAAAGAA AGGCAGCTGG CTGATGCTAG ACGATGTGTG 
CGGTTTGTAG AGGCTGCAGC ACAGGAGAGA GAACAGCAGA AAGAGGCTTC TTGGAAACAT 
AACCAAGAAT TGCAAAAAGC CCTACAGCAG CTACAAGGAG AACTTCAGAG TAAGAGCCAA 
CAGCTTCATA CCCTGGAGGC TGAAAAATAT AATGAGATTC GCACCCAAGA GCAGCACATT 
CAGCACCTCC ACTACAGTCT GAGTCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTACC GAAATAACTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTAGGCAAA GAATACAAGA TAGAGATGTT GCCCTAGAGC GGGCTATAGA TGAAAAGTTC 
TCCAGCCTAG AAGAAAAGGA AAAAGAACTG CGTCAGCTTC ACCTTGCCAT GAGAGAGCGA 
GATCACGACT TAGAGAGGCT GCGCGGTATC CTCTCCGCCA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGTC CAAAGGCCTG GAAGTGGAAC AGTTGTCTGC CACCTGTCAG 
AACCTCCAGT GGCTGAAAGA AGAAATGGAA ACCAAATTTA GCCGTTGGCA GAAGGAGCAA 
GAAAGCATCA TTCAGCAGCT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTTACCGCAA CATTGCTCTG CAAACTTGGA CCAGGGCAGA CTGAGATAGT TGAGGAGCTG 
TGCCAGCGTC TACAGCAGAA GGAAAGGATG CTGCAGGACC TTCTGAGTGA TCGGAACAAG 
CAAGCAGTGG AATATGAAAT GGAGATTCAG GGCCTGCTTC AGTCCATGAG CACCAGAGAG 
CAGGAGAGCC ACGCTGCTGC AGAAAAGATG GTACAAGCTC TAATGGAAAG AAATTCAGAA 
TTACAGGCCC TGCGCCAATA TTTAGGAGGG AAAGATTTCA TGATGTCCCA AGCACCCCTC 
TCTAACCGAC CAGCTGAAGT TACCTCCATC AGCCCCCATC TTGGAGAGCA AACTGATCAA 
GGTTCAGTGC ATATACCCTC CAGAGATGAT AGCACTTCAT TGACTGCCAA AGGGGATGCT 
AGCGTACCCA GGTCCTCGTT AGGGGAGGTG GATACAGTTG CGGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAACT TGAGCTCATG GCTAAAAAAG AGAGAGAAAG TCGGATGGAA 
CTGTCTGCAC TCCAGTCCAT GGTGGCTGTG CAGGAGCAAG AGCTGCAGGT TCAGGCTGCT 
GACATGGAGT CCCTGACCAG GACCATACAG ATTAAGGAAG ACCTCATAAA GGACCTGCAG 
ATGCAGCTGG TTGATCCTGA AGACATCCCA GCTATGGAAC GCCTGACCCA GGAAGTCTTG 
CTTCTTCGGG AAAAAGTGGC TTCAGTAGAA TCACAAGGAC AGGAAACGTC TGGAAACCGA 
AGACAACAAT TGCTGCTGAT GCTCGAAGGA CTGGTAGATG AGCGGAGTCG GCTCAATGAG 
GCCTTACAGG CAGAGAGACA GCTCTATAGC AGTCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGCAAT 
CGGCTAGAAG AAGTTCTCGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCC 
GCCATTGGAG GTACAGCTGC AGGGGACGAC ACGGAGGATG CGAGCACGGA GTTTACCGAC 
AGTATCGAGG AGGAGGCTGC ACACAACAGC CACCAGCAAC TCATCAAGGT GGCGTTGGAG 
AAAAGCCGGG CAGCTGTGGA GACCCAGAAT GTGTCTCCTG CTCCTCCCAC CCTGACAGGA 
GGGGACGGTA ACAGGGGTCT TCAGGAGGAA ATGCTCCACC TGAGGGCTGA AATTCACCGG 
CACTTAGAGG AGAAGAGGAA AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATTGAGGAA 
GACAAGGAGA AGGGCGAGGC GATGGTGGAG ACTGTGGTTG CCAAAGGGGT TCTGAATGAG 
AATAGCCTTC AGGCTGAATT CAGAAAGGTC CAGGGAAAAC TGAAGAATGC CCGCAATATC 
ATCAGCCTCC TCAAAGAACA GCTGGTGTTG AGCAGCAAGG AAGGGACTAG TAAGCTTAAT 
CCTGAGCTCC TTGTGCACCT GGCCAAGGAG ATCAACAGAA TGAACACAGA GCTGGTTTAC 
TCTCCTGGAA AGCACCAATG CCTAGAAGAG GAGGGTGTGA CCACAAGGCC GTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTGCCCTC ACAGTGGATG CTCACCAACT GGATAACCAG 
CCCCAGACCC ATGACCCTGG GCCGCAGTCA GAATTTAGCT TCTCAGGATC CACGAAGCAC 
CTGCGCTCCC AGTTGGCACA ATGCAAACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCAGAAG CCACTGTCTT TGCCCAGGCC AACCAGCTGG AGAAATACAG AGTCATGTTT 
ACAGGTGGAT CGTCGGTGAA GCAGGACAGC AAGCAGGTGC AGGTGGACCT CCAGGACCTG 
GGCTATGAGA CCTGTGGCCG AAGCGAGAAC GAGGCTGAGC GGGAGGAGAG CACCAGCCCT 
GAGTGTGAGG AGCACGACAG CCTCAGGGAA ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG 
CAGGGACACC TGGACTCCAC AATGACCAGC CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA 
CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT GGAAAGTCAG AAGACATCTC TGTCCTACGT 
AAGGACATCA AAGATCTGAA GGCCCAGCTG CAGAACGCCA ACAAGGTCAT TCAGAACCTG 
AAGAGCCGGG TTCGGTCCCT CTCAGTTACA AGCGATTATT CATCTAGTCT GGAGAGACCC 
CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA GGAACTTCAC CGCACAGTGT CACTGATGAG 
GACGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTACC CTCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGGGTG TCCCAGCTAG AAGCCCAGCT CCCAAAAACT 
GGAACGGAAG GGAAGCTGGC CGAGGAGCTG AGATCTGCTT CGTGGCCTGG GAAATATGAT 
TCCCTGATTC AGGACCAGGC CCGAGAACTG TCCTACCTAC GTCAGAAAAT ACGGGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAC TCGAAAGACA CAGTAAAGTC TTTCGAGGAC 
CTCCTAAGGA GCAATGACAT TGACTACTAC CTGGGACAGA GCTTCCGGGA GCACCTCGCC 
CAGGGCAGCC AGCTGACAGA AAGGCTCACC AGCAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT CGAGCCACTG GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATCGAAGT CCTGCAGGCC AAGCTGGACG CCCGCTCCCT CACACCGTGC 
AGCAGCCATG CCTTGTCAGA CTCTCAGCGC TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC 
GAGCTGGAAG CCTGCTCTGA CATGGACATC GCCAGCGAGT ACACACACTA TGAAGAGAAG 
AAGGCTTCAC CTGGTCACTC AGATTCCATC CATCATTCGA GTCATTCTGC TGTACTGTCT 
TCCAAACCAT CAGCAACCAG TTCATCTCAG GAGGTTAAGG CCGAACCCAG CAGCAACCCC 
GTCAGCTTCC CAGCTCCCCA GAATCCCCCC AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTCCAAT CCATACCCAC GCTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC CCTCCCCTCC CTGGCTGCTG TGAGACACCC 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCGGAAGCA GTTAGGAGAA 
AGTGTCACCA GTGTTCATCC TGCTTCCCCA GCCACACTGC CAACCAACCA CACAGAAGCC 
AGATCTTCCC ACTACCTCAA CCCTCCCCAG CCCCTCTGTC CTCCAAGGGG CACCACGGAC 
CTGGGCAGAA TCCTGGAGCC TGGGTACCTG GGTAGCAGTG GCCAGTGGGA CGTGATGAGG 
CCTCAGAAAG GGAGCATATC CGGGGACCTA TCCTCAGGCT CCTCCATGTA CCAGCTTAAC 
TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG GAGCATCTTG GTGAAATCCG GAACCTACGC 
CAGCGCCTGG AGGAGTCCAT CTGCATTAAT GACCGCCTGC GGGAGCAGCT GGAAAACAGG 
CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA ACCTCTAACT TCTACAGTCC AGGCCTGGAG 
TCCACACCTC AGCTCTCCAA TGAGAACAGA GTCCTTAGGG AAGAAAACCA CAGGCTTCAG 
GCTCAGCTCA GTCATGTTTC CAGAGAGCAC TCCCAGGAAA CAGAATGCCT GAGGGAGGCT 
CTGCTGACCT CTCGATCCCG GCTTCAAGAG CTGGAAATGG AGCTGGAGCA CCAAAAGGTA 
GACCGGCAGC AGTTACTGGA AGACCTGAAG GAGAAGCAGC AGGAGATCTT GCATTTCCAG 
GAGGAACGAC TGTCTCTCCA GGAGAAAGAC TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG 
CAGCAACAGT GCGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCAC TTTATGGTGA CTCCAAGAAG ACGCTGAAAG CTTATACCTG GGATGCCTGT 
CACCAAGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC AACTGTCTGC GATTGCAGCT CGAACAGCAG 
TTGGATGGTG GTGCCAGCAA AGCTGGCCTC AGCTCCTCCT CCGTTAACCA GAAATTCCCC 
ACCAACACTG ACCCTGGAAA CAAGCAGCCA TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA 
GTTCGGGATG TGGGCATGAA TTCTCCAGTT CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT 
CCTGGCCCAG AGACCACAAC CTTCAGTAGG ACGAACGAGC TGGGTTTGGA TGCTTCTCCA 
GTAATGAAGA ACCCTCGCAA GCTGGAGGGC GACGCTACTG ATGGCTCCTT TGCCAATAAG 
CATGGCCGCC ACGTCATTGG CCACATTGAT GACTATAGTG CCCTAAGAGA GCAGATTGGG 
GAGGGCAGAC TGCTGGTCAA GAAGATAGCA TCACTGGTGA GACCAACCTG CAGCTTCTCT 
GGTCTTGAAG CTCCAGGCAT AGAGCCACAG GGATGCCCAT GGCTACTGCC CCTTTCCCAG 
GTGATGGGCA GCGAAGGCAT CCAGGAGCTG AGGAGCAGCG CCACCGCCCT GCACCACCGG 
CTGGAGGAGT CGGCCTCCCT CCTCACCATG TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC 
GGCCCTGTGC TTGTGGACAA AGTGGGAGAG TCTATGAAAA AGGAGCTTCT GGAACTGAGA 
ACCAAACTAT CCAAACAGGA AAGTCTCCTT CAGAGTACAT CTGAGCGTCT GAAGACCGCC 
AACCAGCAGA AGGAGAGCAT GGAGCAGTTC ATCTTCAGCC AGTTGACCAG GACACACGAC 
GTTTTGAAGA AGGCAAGGAC TAATTTGGAG AAGACCTCTT ACAAGACTGC TTCTGTAAAG 
CCTTATTCCT GTCCCAGCAA AGGTGAAATC CCTAAGGGCC CTGCCATGCA CTCCAGTCCT 
GTGACCCTTG CCTTCCAGGA GACATGCAAG AAGCGAAGCC ACCAGAGGTC CTTAAAACAG 
CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204115.1
CDS	287..7714
Translation	MEQAWTRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204058.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204058.3

ORF Insert Sequence:

ATGGAGCAGG CGTGGACACG AGATTATTTT GCAGAAGATG ATGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACCAAAGATC GAGGCCCTCC TCTGCAGTCA 
CAGACTTGGA GAAGTGGTGA CAAGATCCCC TTGGGGCATT CCTTGAGAGC ATTTGAAAGA 
CCTCTTCAGG TGCAAACCCA GGCCCTTAGA GACTTTGAGA AGCACCTCAA TGACCTGAAG 
AAGGAGAACT TCAGCCTCAA GCTGCGCATC TACTTCCTGG AGGAGCGCAT GCAGCAGAAG 
TATGAGGCCA GCCGGGATGA CATCTACAAG CGGAACATCG AGCTGAAGGT TGAAGTAGAG 
AGCCTGAAAC GAGAACTCCA GGACAAGAGA CAGCATCCGG ATAAAACATG CGCTGATGCA 
GAGAATATCA ACAGCCATAA TGAAGCTGAG CTCCGGCGCC AGTTTGAGGA GAGACAGCAG 
GAGACGGAGC ATGTTTACGA GCTCCTGGAG AATAAGATCC AGCTTCTGCA AGAGGAGTCC 
AGACTAGCAA AAAATGAAGC CGCACGGATG GCGGCTCTGG TGGAAGCAGA GAAAGAGTGT 
AACCTGGAGC TCTCAGAGAA ACTGAAGGGA GTCACCAAGG AGAGGGAAGA TGTACCAGGA 
GACCGGGTCA AATCTGACCA GTACACTGAG GCCCTGGCCC AGAGGGACAA AAAAATTGAA 
GAACTGAGTC AAAGCCTGGC TGCACAGGAG AAACTTGTAG AGCAGCTGTC TCAAGAGAAA 
CAACAACTGC TACATCTGTT GGAGGAGCCA GCTGACATGG AAGTGCAGCC CATGACTGAA 
GACTTACTCA AACAACAGAA GTTGAATTCA AATGAGACCG GTACAACTCA GCAGTCTGTA 
TCTGATTCCC ATCTGGCAGA ACTCCAGGAA AAAATCCAGC AAACAGAGAC CACCAACAAG 
ATGCTTCAAG AAAAACTGAA TGAAATGAGC TATGAACTGA AGTCTGCTCA GGAGTCATCT 
CAGAAGCAAG ATGATACAAT TCAAAACCTC AAGGAAACTC TGAAAAGCAG GGAAAGTGAG 
ACCGAGGAGT TGTACCAGGT GATTGAAGGT CAAAACGACA CAATGGCTAA GCTTCGAGAA 
ATGCTGCACC AGAGTCAGCT TAAACAGCTT CATAACTCAG AGGGTACTTC CCCAGCTCAG 
CAACAGGTGG CTCTGCTTGA TCTTCAAAGT GCTTTATTCT GCAGCCAGCT TGAAATACAG 
AAGCTCCAGA GGGCAGTACG CCAAAAAGAA AGGCAGCTGG CTGATGCTAG ACGATGTGTG 
CGGTTTGTAG AGGCTGCAGC ACAGGAGAGA GAACAGCAGA AAGAGGCTTC TTGGAAACAT 
AACCAAGAAT TGCAAAAAGC CCTACAGCAG CTACAAGGAG AACTTCAGAG TAAGAGCCAA 
CAGCTTCATA CCCTGGAGGC TGAAAAATAT AATGAGATTC GCACCCAAGA GCAGCACATT 
CAGCACCTCC ACTACAGTCT GAGTCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTACC GAAATAACTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTAGGCAAA GAATACAAGA TAGAGATGTT GCCCTAGAGC GGGCTATAGA TGAAAAGTTC 
TCCAGCCTAG AAGAAAAGGA AAAAGAACTG CGTCAGCTTC ACCTTGCCAT GAGAGAGCGA 
GATCACGACT TAGAGAGGCT GCGCGGTATC CTCTCCGCCA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGTC CAAAGGCCTG GAAGTGGAAC AGTTGTCTGC CACCTGTCAG 
AACCTCCAGT GGCTGAAAGA AGAAATGGAA ACCAAATTTA GCCGTTGGCA GAAGGAGCAA 
GAAAGCATCA TTCAGCAGCT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTTACCGCAA CATTGCTCTG CAAACTTGGA CCAGGGCAGA CTGAGATAGT TGAGGAGCTG 
TGCCAGCGTC TACAGCAGAA GGAAAGGATG CTGCAGGACC TTCTGAGTGA TCGGAACAAG 
CAAGCAGTGG AATATGAAAT GGAGATTCAG GGCCTGCTTC AGTCCATGAG CACCAGAGAG 
CAGGAGAGCC ACGCTGCTGC AGAAAAGATG GTACAAGCTC TAATGGAAAG AAATTCAGAA 
TTACAGGCCC TGCGCCAATA TTTAGGAGGG AAAGATTTCA TGATGTCCCA AGCACCCCTC 
TCTAACCGAC CAGCTGAAGT TACCTCCATC AGCCCCCATC TTGGAGAGCA AACTGATCAA 
GGTTCAGTGC ATATACCCTC CAGAGATGAT AGCACTTCAT TGACTGCCAA AGGGGATGCT 
AGCGTACCCA GGTCCTCGTT AGGGGAGGTG GATACAGTTG CGGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAACT TGAGCTCATG GCTAAAAAAG AGAGAGAAAG TCGGATGGAA 
CTGTCTGCAC TCCAGTCCAT GGTGGCTGTG CAGGAGCAAG AGCTGCAGGT TCAGGCTGCT 
GACATGGAGT CCCTGACCAG GACCATACAG ATTAAGGAAG ACCTCATAAA GGACCTGCAG 
ATGCAGCTGG TTGATCCTGA AGACATCCCA GCTATGGAAC GCCTGACCCA GGAAGTCTTG 
CTTCTTCGGG AAAAAGTGGC TTCAGTAGAA TCACAAGGAC AGGAAACGTC TGGAAACCGA 
AGACAACAAT TGCTGCTGAT GCTCGAAGGA CTGGTAGATG AGCGGAGTCG GCTCAATGAG 
GCCTTACAGG CAGAGAGACA GCTCTATAGC AGTCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGCAAT 
CGGCTAGAAG AAGTTCTCGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCC 
GCCATTGGAG GTACAGCTGC AGGGGACGAC ACGGAGGATG CGAGCACGGA GTTTACCGAC 
AGTATCGAGG AGGAGGCTGC ACACAACAGC CACCAGCAAC TCATCAAGGT GGCGTTGGAG 
AAAAGCCGGG CAGCTGTGGA GACCCAGAAT GTGTCTCCTG CTCCTCCCAC CCTGACAGGA 
GGGGACGGTA ACAGGGGTCT TCAGGAGGAA ATGCTCCACC TGAGGGCTGA AATTCACCGG 
CACTTAGAGG AGAAGAGGAA AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATTGAGGAA 
GACAAGGAGA AGGGCGAGGC GATGGTGGAG ACTGTGGTTG CCAAAGGGGT TCTGAATGAG 
AATAGCCTTC AGGCTGAATT CAGAAAGGTC CAGGGAAAAC TGAAGAATGC CCGCAATATC 
ATCAGCCTCC TCAAAGAACA GCTGGTGTTG AGCAGCAAGG AAGGGACTAG TAAGCTTAAT 
CCTGAGCTCC TTGTGCACCT GGCCAAGGAG ATCAACAGAA TGAACACAGA GCTGGTTTAC 
TCTCCTGGAA AGCACCAATG CCTAGAAGAG GAGGGTGTGA CCACAAGGCC GTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTGCCCTC ACAGTGGATG CTCACCAACT GGATAACCAG 
CCCCAGACCC ATGACCCTGG GCCGCAGTCA GAATTTAGCT TCTCAGGATC CACGAAGCAC 
CTGCGCTCCC AGTTGGCACA ATGCAAACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCAGAAG CCACTGTCTT TGCCCAGGCC AACCAGCTGG AGAAATACAG AGTCATGTTT 
ACAGGTGGAT CGTCGGTGAA GCAGGACAGC AAGCAGGTGC AGGTGGACCT CCAGGACCTG 
GGCTATGAGA CCTGTGGCCG AAGCGAGAAC GAGGCTGAGC GGGAGGAGAG CACCAGCCCT 
GAGTGTGAGG AGCACGACAG CCTCAGGGAA ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG 
CAGGGACACC TGGACTCCAC AATGACCAGC CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA 
CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT GGAAAGTCAG AAGACATCTC TGTCCTACGT 
AAGGACATCA AAGATCTGAA GGCCCAGCTG CAGAACGCCA ACAAGGTCAT TCAGAACCTG 
AAGAGCCGGG TTCGGTCCCT CTCAGTTACA AGCGATTATT CATCTAGTCT GGAGAGACCC 
CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA GGAACTTCAC CGCACAGTGT CACTGATGAG 
GACGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTACC CTCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGGGTG TCCCAGCTAG AAGCCCAGCT CCCAAAAACT 
GGAACGGAAG GGAAGCTGGC CGAGGAGCTG AGATCTGCTT CGTGGCCTGG GAAATATGAT 
TCCCTGATTC AGGACCAGGC CCGAGAACTG TCCTACCTAC GTCAGAAAAT ACGGGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAC TCGAAAGACA CAGTAAAGTC TTTCGAGGAC 
CTCCTAAGGA GCAATGACAT TGACTACTAC CTGGGACAGA GCTTCCGGGA GCACCTCGCC 
CAGGGCAGCC AGCTGACAGA AAGGCTCACC AGCAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT CGAGCCACTG GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATCGAAGT CCTGCAGGCC AAGCTGGACG CCCGCTCCCT CACACCGTGC 
AGCAGCCATG CCTTGTCAGA CTCTCAGCGC TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC 
GAGCTGGAAG CCTGCTCTGA CATGGACATC GCCAGCGAGT ACACACACTA TGAAGAGAAG 
AAGGCTTCAC CTGGTCACTC AGATTCCATC CATCATTCGA GTCATTCTGC TGTACTGTCT 
TCCAAACCAT CAGCAACCAG TTCATCTCAG GAGGTTAAGG CCGAACCCAG CAGCAACCCC 
GTCAGCTTCC CAGCTCCCCA GAATCCCCCC AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTCCAAT CCATACCCAC GCTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC CCTCCCCTCC CTGGCTGCTG TGAGACACCC 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCGGAAGCA GTTAGGAGAA 
AGTGTCACCA GTGTTCATCC TGCTTCCCCA GCCACACTGC CAACCAACCA CACAGAAGCC 
AGATCTTCCC ACTACCTCAA CCCTCCCCAG CCCCTCTGTC CTCCAAGGGG CACCACGGAC 
CTGGGCAGAA TCCTGGAGCC TGGGTACCTG GGTAGCAGTG GCCAGTGGGA CGTGATGAGG 
CCTCAGAAAG GGAGCATATC CGGGGACCTA TCCTCAGGCT CCTCCATGTA CCAGCTTAAC 
TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG GAGCATCTTG GTGAAATCCG GAACCTACGC 
CAGCGCCTGG AGGAGTCCAT CTGCATTAAT GACCGCCTGC GGGAGCAGCT GGAAAACAGG 
CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA ACCTCTAACT TCTACAGTCC AGGCCTGGAG 
TCCACACCTC AGCTCTCCAA TGAGAACAGA GTCCTTAGGG AAGAAAACCA CAGGCTTCAG 
GCTCAGCTCA GTCATGTTTC CAGAGAGCAC TCCCAGGAAA CAGAATGCCT GAGGGAGGCT 
CTGCTGACCT CTCGATCCCG GCTTCAAGAG CTGGAAATGG AGCTGGAGCA CCAAAAGGTA 
GACCGGCAGC AGTTACTGGA AGACCTGAAG GAGAAGCAGC AGGAGATCTT GCATTTCCAG 
GAGGAACGAC TGTCTCTCCA GGAGAAAGAC TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG 
CAGCAACAGT GCGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCAC TTTATGGTGA CTCCAAGAAG ACGCTGAAAG CTTATACCTG GGATGCCTGT 
CACCAAGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC AACTGTCTGC GATTGCAGCT CGAACAGCAG 
TTGGATGGTG GTGCCAGCAA AGCTGGCCTC AGCTCCTCCT CCGTTAACCA GAAATTCCCC 
ACCAACACTG ACCCTGGAAA CAAGCAGCCA TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA 
GTTCGGGATG TGGGCATGAA TTCTCCAGTT CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT 
CCTGGCCCAG AGACCACAAC CTTCAGTAGG ACGAACGAGC TGGGTTTGGA TGCTTCTCCA 
GTAATGAAGA ACCCTCGCAA GCTGGAGGGC GACGCTACTG ATGGCTCCTT TGCCAATAAG 
CATGGCCGCC ACGTCATTGG CCACATTGAT GACTATAGTG CCCTAAGAGA GCAGATTGGG 
GAGGGCAGAC TGCTGGTCAA GAAGATAGCA TCACTGGTGA GACCAACCTG CAGCTTCTCT 
GGTCTTGAAG CTCCAGGCAT AGAGCCACAG GGATGCCCAT GGCTACTGCC CCTTTCCCAG 
GTGATGGGCA GCGAAGGCAT CCAGGAGCTG AGGAGCAGCG CCACCGCCCT GCACCACCGG 
CTGGAGGAGT CGGCCTCCCT CCTCACCATG TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC 
GGCCCTGTGC TTGTGGACAA AGTGGGAGAG TCTATGAAAA AGGAGCTTCT GGAACTGAGA 
ACCAAACTAT CCAAACAGGA AAGTCTCCTT CAGAGTACAT CTGAGCGTCT GAAGACCGCC 
AACCAGCAGA AGGAGAGCAT GGAGCAGTTC ATCTTCAGCC AGTTGACCAG GACACACGAC 
GTTTTGAAGA AGGCAAGGAC TAATTTGGAG AAGACCTCTT ACAAGACTGC TTCTGTAAAG 
CCTTATTCCT GTCCCAGCAA AGGTGAAATC CCTAAGGGCC CTGCCATGCA CTCCAGTCCT 
GTGACCCTTG CCTTCCAGGA GACATGCAAG AAGCGAAGCC ACCAGAGGTC CTTAAAACAG 
CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826853.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825155.1 Bos taurus myomegalin (LOC508547), transcript variant X13, mRNA.

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CloneID	OBa17544
Clone ID Related Accession (Same CDS sequence)	XM_005204058.3 , XM_010826853.2 , XM_005204058.4
Accession Version	XM_010826853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CGTGGACACG AGATTATTTT GCAGAAGATG ATGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACCAAAGATC GAGGCCCTCC TCTGCAGTCA 
CAGACTTGGA GAAGTGGTGA CAAGATCCCC TTGGGGCATT CCTTGAGAGC ATTTGAAAGA 
CCTCTTCAGG TGCAAACCCA GGCCCTTAGA GACTTTGAGA AGCACCTCAA TGACCTGAAG 
AAGGAGAACT TCAGCCTCAA GCTGCGCATC TACTTCCTGG AGGAGCGCAT GCAGCAGAAG 
TATGAGGCCA GCCGGGATGA CATCTACAAG CGGAACATCG AGCTGAAGGT TGAAGTAGAG 
AGCCTGAAAC GAGAACTCCA GGACAAGAGA CAGCATCCGG ATAAAACATG CGCTGATGCA 
GAGAATATCA ACAGCCATAA TGAAGCTGAG CTCCGGCGCC AGTTTGAGGA GAGACAGCAG 
GAGACGGAGC ATGTTTACGA GCTCCTGGAG AATAAGATCC AGCTTCTGCA AGAGGAGTCC 
AGACTAGCAA AAAATGAAGC CGCACGGATG GCGGCTCTGG TGGAAGCAGA GAAAGAGTGT 
AACCTGGAGC TCTCAGAGAA ACTGAAGGGA GTCACCAAGG AGAGGGAAGA TGTACCAGGA 
GACCGGGTCA AATCTGACCA GTACACTGAG GCCCTGGCCC AGAGGGACAA AAAAATTGAA 
GAACTGAGTC AAAGCCTGGC TGCACAGGAG AAACTTGTAG AGCAGCTGTC TCAAGAGAAA 
CAACAACTGC TACATCTGTT GGAGGAGCCA GCTGACATGG AAGTGCAGCC CATGACTGAA 
GACTTACTCA AACAACAGAA GTTGAATTCA AATGAGACCG GTACAACTCA GCAGTCTGTA 
TCTGATTCCC ATCTGGCAGA ACTCCAGGAA AAAATCCAGC AAACAGAGAC CACCAACAAG 
ATGCTTCAAG AAAAACTGAA TGAAATGAGC TATGAACTGA AGTCTGCTCA GGAGTCATCT 
CAGAAGCAAG ATGATACAAT TCAAAACCTC AAGGAAACTC TGAAAAGCAG GGAAAGTGAG 
ACCGAGGAGT TGTACCAGGT GATTGAAGGT CAAAACGACA CAATGGCTAA GCTTCGAGAA 
ATGCTGCACC AGAGTCAGCT TAAACAGCTT CATAACTCAG AGGGTACTTC CCCAGCTCAG 
CAACAGGTGG CTCTGCTTGA TCTTCAAAGT GCTTTATTCT GCAGCCAGCT TGAAATACAG 
AAGCTCCAGA GGGCAGTACG CCAAAAAGAA AGGCAGCTGG CTGATGCTAG ACGATGTGTG 
CGGTTTGTAG AGGCTGCAGC ACAGGAGAGA GAACAGCAGA AAGAGGCTTC TTGGAAACAT 
AACCAAGAAT TGCAAAAAGC CCTACAGCAG CTACAAGGAG AACTTCAGAG TAAGAGCCAA 
CAGCTTCATA CCCTGGAGGC TGAAAAATAT AATGAGATTC GCACCCAAGA GCAGCACATT 
CAGCACCTCC ACTACAGTCT GAGTCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTACC GAAATAACTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTAGGCAAA GAATACAAGA TAGAGATGTT GCCCTAGAGC GGGCTATAGA TGAAAAGTTC 
TCCAGCCTAG AAGAAAAGGA AAAAGAACTG CGTCAGCTTC ACCTTGCCAT GAGAGAGCGA 
GATCACGACT TAGAGAGGCT GCGCGGTATC CTCTCCGCCA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGTC CAAAGGCCTG GAAGTGGAAC AGTTGTCTGC CACCTGTCAG 
AACCTCCAGT GGCTGAAAGA AGAAATGGAA ACCAAATTTA GCCGTTGGCA GAAGGAGCAA 
GAAAGCATCA TTCAGCAGCT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTTACCGCAA CATTGCTCTG CAAACTTGGA CCAGGGCAGA CTGAGATAGT TGAGGAGCTG 
TGCCAGCGTC TACAGCAGAA GGAAAGGATG CTGCAGGACC TTCTGAGTGA TCGGAACAAG 
CAAGCAGTGG AATATGAAAT GGAGATTCAG GGCCTGCTTC AGTCCATGAG CACCAGAGAG 
CAGGAGAGCC ACGCTGCTGC AGAAAAGATG GTACAAGCTC TAATGGAAAG AAATTCAGAA 
TTACAGGCCC TGCGCCAATA TTTAGGAGGG AAAGATTTCA TGATGTCCCA AGCACCCCTC 
TCTAACCGAC CAGCTGAAGT TACCTCCATC AGCCCCCATC TTGGAGAGCA AACTGATCAA 
GGTTCAGTGC ATATACCCTC CAGAGATGAT AGCACTTCAT TGACTGCCAA AGGGGATGCT 
AGCGTACCCA GGTCCTCGTT AGGGGAGGTG GATACAGTTG CGGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAACT TGAGCTCATG GCTAAAAAAG AGAGAGAAAG TCGGATGGAA 
CTGTCTGCAC TCCAGTCCAT GGTGGCTGTG CAGGAGCAAG AGCTGCAGGT TCAGGCTGCT 
GACATGGAGT CCCTGACCAG GACCATACAG ATTAAGGAAG ACCTCATAAA GGACCTGCAG 
ATGCAGCTGG TTGATCCTGA AGACATCCCA GCTATGGAAC GCCTGACCCA GGAAGTCTTG 
CTTCTTCGGG AAAAAGTGGC TTCAGTAGAA TCACAAGGAC AGGAAACGTC TGGAAACCGA 
AGACAACAAT TGCTGCTGAT GCTCGAAGGA CTGGTAGATG AGCGGAGTCG GCTCAATGAG 
GCCTTACAGG CAGAGAGACA GCTCTATAGC AGTCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGCAAT 
CGGCTAGAAG AAGTTCTCGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCC 
GCCATTGGAG GTACAGCTGC AGGGGACGAC ACGGAGGATG CGAGCACGGA GTTTACCGAC 
AGTATCGAGG AGGAGGCTGC ACACAACAGC CACCAGCAAC TCATCAAGGT GGCGTTGGAG 
AAAAGCCGGG CAGCTGTGGA GACCCAGAAT GTGTCTCCTG CTCCTCCCAC CCTGACAGGA 
GGGGACGGTA ACAGGGGTCT TCAGGAGGAA ATGCTCCACC TGAGGGCTGA AATTCACCGG 
CACTTAGAGG AGAAGAGGAA AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATTGAGGAA 
GACAAGGAGA AGGGCGAGGC GATGGTGGAG ACTGTGGTTG CCAAAGGGGT TCTGAATGAG 
AATAGCCTTC AGGCTGAATT CAGAAAGGTC CAGGGAAAAC TGAAGAATGC CCGCAATATC 
ATCAGCCTCC TCAAAGAACA GCTGGTGTTG AGCAGCAAGG AAGGGACTAG TAAGCTTAAT 
CCTGAGCTCC TTGTGCACCT GGCCAAGGAG ATCAACAGAA TGAACACAGA GCTGGTTTAC 
TCTCCTGGAA AGCACCAATG CCTAGAAGAG GAGGGTGTGA CCACAAGGCC GTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTGCCCTC ACAGTGGATG CTCACCAACT GGATAACCAG 
CCCCAGACCC ATGACCCTGG GCCGCAGTCA GAATTTAGCT TCTCAGGATC CACGAAGCAC 
CTGCGCTCCC AGTTGGCACA ATGCAAACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCAGAAG CCACTGTCTT TGCCCAGGCC AACCAGCTGG AGAAATACAG AGTCATGTTT 
ACAGGTGGAT CGTCGGTGAA GCAGGACAGC AAGCAGGTGC AGGTGGACCT CCAGGACCTG 
GGCTATGAGA CCTGTGGCCG AAGCGAGAAC GAGGCTGAGC GGGAGGAGAG CACCAGCCCT 
GAGTGTGAGG AGCACGACAG CCTCAGGGAA ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG 
CAGGGACACC TGGACTCCAC AATGACCAGC CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA 
CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT GGAAAGTCAG AAGACATCTC TGTCCTACGT 
AAGGACATCA AAGATCTGAA GGCCCAGCTG CAGAACGCCA ACAAGGTCAT TCAGAACCTG 
AAGAGCCGGG TTCGGTCCCT CTCAGTTACA AGCGATTATT CATCTAGTCT GGAGAGACCC 
CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA GGAACTTCAC CGCACAGTGT CACTGATGAG 
GACGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTACC CTCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGGGTG TCCCAGCTAG AAGCCCAGCT CCCAAAAACT 
GGAACGGAAG GGAAGCTGGC CGAGGAGCTG AGATCTGCTT CGTGGCCTGG GAAATATGAT 
TCCCTGATTC AGGACCAGGC CCGAGAACTG TCCTACCTAC GTCAGAAAAT ACGGGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAC TCGAAAGACA CAGTAAAGTC TTTCGAGGAC 
CTCCTAAGGA GCAATGACAT TGACTACTAC CTGGGACAGA GCTTCCGGGA GCACCTCGCC 
CAGGGCAGCC AGCTGACAGA AAGGCTCACC AGCAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT CGAGCCACTG GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATCGAAGT CCTGCAGGCC AAGCTGGACG CCCGCTCCCT CACACCGTGC 
AGCAGCCATG CCTTGTCAGA CTCTCAGCGC TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC 
GAGCTGGAAG CCTGCTCTGA CATGGACATC GCCAGCGAGT ACACACACTA TGAAGAGAAG 
AAGGCTTCAC CTGGTCACTC AGATTCCATC CATCATTCGA GTCATTCTGC TGTACTGTCT 
TCCAAACCAT CAGCAACCAG TTCATCTCAG GAGGTTAAGG CCGAACCCAG CAGCAACCCC 
GTCAGCTTCC CAGCTCCCCA GAATCCCCCC AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTCCAAT CCATACCCAC GCTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC CCTCCCCTCC CTGGCTGCTG TGAGACACCC 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCGGAAGCA GTTAGGAGAA 
AGTGTCACCA GTGTTCATCC TGCTTCCCCA GCCACACTGC CAACCAACCA CACAGAAGCC 
AGATCTTCCC ACTACCTCAA CCCTCCCCAG CCCCTCTGTC CTCCAAGGGG CACCACGGAC 
CTGGGCAGAA TCCTGGAGCC TGGGTACCTG GGTAGCAGTG GCCAGTGGGA CGTGATGAGG 
CCTCAGAAAG GGAGCATATC CGGGGACCTA TCCTCAGGCT CCTCCATGTA CCAGCTTAAC 
TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG GAGCATCTTG GTGAAATCCG GAACCTACGC 
CAGCGCCTGG AGGAGTCCAT CTGCATTAAT GACCGCCTGC GGGAGCAGCT GGAAAACAGG 
CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA ACCTCTAACT TCTACAGTCC AGGCCTGGAG 
TCCACACCTC AGCTCTCCAA TGAGAACAGA GTCCTTAGGG AAGAAAACCA CAGGCTTCAG 
GCTCAGCTCA GTCATGTTTC CAGAGAGCAC TCCCAGGAAA CAGAATGCCT GAGGGAGGCT 
CTGCTGACCT CTCGATCCCG GCTTCAAGAG CTGGAAATGG AGCTGGAGCA CCAAAAGGTA 
GACCGGCAGC AGTTACTGGA AGACCTGAAG GAGAAGCAGC AGGAGATCTT GCATTTCCAG 
GAGGAACGAC TGTCTCTCCA GGAGAAAGAC TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG 
CAGCAACAGT GCGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCAC TTTATGGTGA CTCCAAGAAG ACGCTGAAAG CTTATACCTG GGATGCCTGT 
CACCAAGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC AACTGTCTGC GATTGCAGCT CGAACAGCAG 
TTGGATGGTG GTGCCAGCAA AGCTGGCCTC AGCTCCTCCT CCGTTAACCA GAAATTCCCC 
ACCAACACTG ACCCTGGAAA CAAGCAGCCA TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA 
GTTCGGGATG TGGGCATGAA TTCTCCAGTT CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT 
CCTGGCCCAG AGACCACAAC CTTCAGTAGG ACGAACGAGC TGGGTTTGGA TGCTTCTCCA 
GTAATGAAGA ACCCTCGCAA GCTGGAGGGC GACGCTACTG ATGGCTCCTT TGCCAATAAG 
CATGGCCGCC ACGTCATTGG CCACATTGAT GACTATAGTG CCCTAAGAGA GCAGATTGGG 
GAGGGCAGAC TGCTGGTCAA GAAGATAGCA TCACTGGTGA GACCAACCTG CAGCTTCTCT 
GGTCTTGAAG CTCCAGGCAT AGAGCCACAG GGATGCCCAT GGCTACTGCC CCTTTCCCAG 
GTGATGGGCA GCGAAGGCAT CCAGGAGCTG AGGAGCAGCG CCACCGCCCT GCACCACCGG 
CTGGAGGAGT CGGCCTCCCT CCTCACCATG TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC 
GGCCCTGTGC TTGTGGACAA AGTGGGAGAG TCTATGAAAA AGGAGCTTCT GGAACTGAGA 
ACCAAACTAT CCAAACAGGA AAGTCTCCTT CAGAGTACAT CTGAGCGTCT GAAGACCGCC 
AACCAGCAGA AGGAGAGCAT GGAGCAGTTC ATCTTCAGCC AGTTGACCAG GACACACGAC 
GTTTTGAAGA AGGCAAGGAC TAATTTGGAG AAGACCTCTT ACAAGACTGC TTCTGTAAAG 
CCTTATTCCT GTCCCAGCAA AGGTGAAATC CCTAAGGGCC CTGCCATGCA CTCCAGTCCT 
GTGACCCTTG CCTTCCAGGA GACATGCAAG AAGCGAAGCC ACCAGAGGTC CTTAAAACAG 
CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825155.1
CDS	287..7714
Translation	MEQAWTRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010826853.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826853.2

ORF Insert Sequence:

ATGGAGCAGG CGTGGACACG AGATTATTTT GCAGAAGATG ATGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACCAAAGATC GAGGCCCTCC TCTGCAGTCA 
CAGACTTGGA GAAGTGGTGA CAAGATCCCC TTGGGGCATT CCTTGAGAGC ATTTGAAAGA 
CCTCTTCAGG TGCAAACCCA GGCCCTTAGA GACTTTGAGA AGCACCTCAA TGACCTGAAG 
AAGGAGAACT TCAGCCTCAA GCTGCGCATC TACTTCCTGG AGGAGCGCAT GCAGCAGAAG 
TATGAGGCCA GCCGGGATGA CATCTACAAG CGGAACATCG AGCTGAAGGT TGAAGTAGAG 
AGCCTGAAAC GAGAACTCCA GGACAAGAGA CAGCATCCGG ATAAAACATG CGCTGATGCA 
GAGAATATCA ACAGCCATAA TGAAGCTGAG CTCCGGCGCC AGTTTGAGGA GAGACAGCAG 
GAGACGGAGC ATGTTTACGA GCTCCTGGAG AATAAGATCC AGCTTCTGCA AGAGGAGTCC 
AGACTAGCAA AAAATGAAGC CGCACGGATG GCGGCTCTGG TGGAAGCAGA GAAAGAGTGT 
AACCTGGAGC TCTCAGAGAA ACTGAAGGGA GTCACCAAGG AGAGGGAAGA TGTACCAGGA 
GACCGGGTCA AATCTGACCA GTACACTGAG GCCCTGGCCC AGAGGGACAA AAAAATTGAA 
GAACTGAGTC AAAGCCTGGC TGCACAGGAG AAACTTGTAG AGCAGCTGTC TCAAGAGAAA 
CAACAACTGC TACATCTGTT GGAGGAGCCA GCTGACATGG AAGTGCAGCC CATGACTGAA 
GACTTACTCA AACAACAGAA GTTGAATTCA AATGAGACCG GTACAACTCA GCAGTCTGTA 
TCTGATTCCC ATCTGGCAGA ACTCCAGGAA AAAATCCAGC AAACAGAGAC CACCAACAAG 
ATGCTTCAAG AAAAACTGAA TGAAATGAGC TATGAACTGA AGTCTGCTCA GGAGTCATCT 
CAGAAGCAAG ATGATACAAT TCAAAACCTC AAGGAAACTC TGAAAAGCAG GGAAAGTGAG 
ACCGAGGAGT TGTACCAGGT GATTGAAGGT CAAAACGACA CAATGGCTAA GCTTCGAGAA 
ATGCTGCACC AGAGTCAGCT TAAACAGCTT CATAACTCAG AGGGTACTTC CCCAGCTCAG 
CAACAGGTGG CTCTGCTTGA TCTTCAAAGT GCTTTATTCT GCAGCCAGCT TGAAATACAG 
AAGCTCCAGA GGGCAGTACG CCAAAAAGAA AGGCAGCTGG CTGATGCTAG ACGATGTGTG 
CGGTTTGTAG AGGCTGCAGC ACAGGAGAGA GAACAGCAGA AAGAGGCTTC TTGGAAACAT 
AACCAAGAAT TGCAAAAAGC CCTACAGCAG CTACAAGGAG AACTTCAGAG TAAGAGCCAA 
CAGCTTCATA CCCTGGAGGC TGAAAAATAT AATGAGATTC GCACCCAAGA GCAGCACATT 
CAGCACCTCC ACTACAGTCT GAGTCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTACC GAAATAACTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTAGGCAAA GAATACAAGA TAGAGATGTT GCCCTAGAGC GGGCTATAGA TGAAAAGTTC 
TCCAGCCTAG AAGAAAAGGA AAAAGAACTG CGTCAGCTTC ACCTTGCCAT GAGAGAGCGA 
GATCACGACT TAGAGAGGCT GCGCGGTATC CTCTCCGCCA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGTC CAAAGGCCTG GAAGTGGAAC AGTTGTCTGC CACCTGTCAG 
AACCTCCAGT GGCTGAAAGA AGAAATGGAA ACCAAATTTA GCCGTTGGCA GAAGGAGCAA 
GAAAGCATCA TTCAGCAGCT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTTACCGCAA CATTGCTCTG CAAACTTGGA CCAGGGCAGA CTGAGATAGT TGAGGAGCTG 
TGCCAGCGTC TACAGCAGAA GGAAAGGATG CTGCAGGACC TTCTGAGTGA TCGGAACAAG 
CAAGCAGTGG AATATGAAAT GGAGATTCAG GGCCTGCTTC AGTCCATGAG CACCAGAGAG 
CAGGAGAGCC ACGCTGCTGC AGAAAAGATG GTACAAGCTC TAATGGAAAG AAATTCAGAA 
TTACAGGCCC TGCGCCAATA TTTAGGAGGG AAAGATTTCA TGATGTCCCA AGCACCCCTC 
TCTAACCGAC CAGCTGAAGT TACCTCCATC AGCCCCCATC TTGGAGAGCA AACTGATCAA 
GGTTCAGTGC ATATACCCTC CAGAGATGAT AGCACTTCAT TGACTGCCAA AGGGGATGCT 
AGCGTACCCA GGTCCTCGTT AGGGGAGGTG GATACAGTTG CGGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAACT TGAGCTCATG GCTAAAAAAG AGAGAGAAAG TCGGATGGAA 
CTGTCTGCAC TCCAGTCCAT GGTGGCTGTG CAGGAGCAAG AGCTGCAGGT TCAGGCTGCT 
GACATGGAGT CCCTGACCAG GACCATACAG ATTAAGGAAG ACCTCATAAA GGACCTGCAG 
ATGCAGCTGG TTGATCCTGA AGACATCCCA GCTATGGAAC GCCTGACCCA GGAAGTCTTG 
CTTCTTCGGG AAAAAGTGGC TTCAGTAGAA TCACAAGGAC AGGAAACGTC TGGAAACCGA 
AGACAACAAT TGCTGCTGAT GCTCGAAGGA CTGGTAGATG AGCGGAGTCG GCTCAATGAG 
GCCTTACAGG CAGAGAGACA GCTCTATAGC AGTCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGCAAT 
CGGCTAGAAG AAGTTCTCGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCC 
GCCATTGGAG GTACAGCTGC AGGGGACGAC ACGGAGGATG CGAGCACGGA GTTTACCGAC 
AGTATCGAGG AGGAGGCTGC ACACAACAGC CACCAGCAAC TCATCAAGGT GGCGTTGGAG 
AAAAGCCGGG CAGCTGTGGA GACCCAGAAT GTGTCTCCTG CTCCTCCCAC CCTGACAGGA 
GGGGACGGTA ACAGGGGTCT TCAGGAGGAA ATGCTCCACC TGAGGGCTGA AATTCACCGG 
CACTTAGAGG AGAAGAGGAA AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATTGAGGAA 
GACAAGGAGA AGGGCGAGGC GATGGTGGAG ACTGTGGTTG CCAAAGGGGT TCTGAATGAG 
AATAGCCTTC AGGCTGAATT CAGAAAGGTC CAGGGAAAAC TGAAGAATGC CCGCAATATC 
ATCAGCCTCC TCAAAGAACA GCTGGTGTTG AGCAGCAAGG AAGGGACTAG TAAGCTTAAT 
CCTGAGCTCC TTGTGCACCT GGCCAAGGAG ATCAACAGAA TGAACACAGA GCTGGTTTAC 
TCTCCTGGAA AGCACCAATG CCTAGAAGAG GAGGGTGTGA CCACAAGGCC GTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTGCCCTC ACAGTGGATG CTCACCAACT GGATAACCAG 
CCCCAGACCC ATGACCCTGG GCCGCAGTCA GAATTTAGCT TCTCAGGATC CACGAAGCAC 
CTGCGCTCCC AGTTGGCACA ATGCAAACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCAGAAG CCACTGTCTT TGCCCAGGCC AACCAGCTGG AGAAATACAG AGTCATGTTT 
ACAGGTGGAT CGTCGGTGAA GCAGGACAGC AAGCAGGTGC AGGTGGACCT CCAGGACCTG 
GGCTATGAGA CCTGTGGCCG AAGCGAGAAC GAGGCTGAGC GGGAGGAGAG CACCAGCCCT 
GAGTGTGAGG AGCACGACAG CCTCAGGGAA ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG 
CAGGGACACC TGGACTCCAC AATGACCAGC CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA 
CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT GGAAAGTCAG AAGACATCTC TGTCCTACGT 
AAGGACATCA AAGATCTGAA GGCCCAGCTG CAGAACGCCA ACAAGGTCAT TCAGAACCTG 
AAGAGCCGGG TTCGGTCCCT CTCAGTTACA AGCGATTATT CATCTAGTCT GGAGAGACCC 
CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA GGAACTTCAC CGCACAGTGT CACTGATGAG 
GACGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTACC CTCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGGGTG TCCCAGCTAG AAGCCCAGCT CCCAAAAACT 
GGAACGGAAG GGAAGCTGGC CGAGGAGCTG AGATCTGCTT CGTGGCCTGG GAAATATGAT 
TCCCTGATTC AGGACCAGGC CCGAGAACTG TCCTACCTAC GTCAGAAAAT ACGGGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAC TCGAAAGACA CAGTAAAGTC TTTCGAGGAC 
CTCCTAAGGA GCAATGACAT TGACTACTAC CTGGGACAGA GCTTCCGGGA GCACCTCGCC 
CAGGGCAGCC AGCTGACAGA AAGGCTCACC AGCAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT CGAGCCACTG GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATCGAAGT CCTGCAGGCC AAGCTGGACG CCCGCTCCCT CACACCGTGC 
AGCAGCCATG CCTTGTCAGA CTCTCAGCGC TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC 
GAGCTGGAAG CCTGCTCTGA CATGGACATC GCCAGCGAGT ACACACACTA TGAAGAGAAG 
AAGGCTTCAC CTGGTCACTC AGATTCCATC CATCATTCGA GTCATTCTGC TGTACTGTCT 
TCCAAACCAT CAGCAACCAG TTCATCTCAG GAGGTTAAGG CCGAACCCAG CAGCAACCCC 
GTCAGCTTCC CAGCTCCCCA GAATCCCCCC AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTCCAAT CCATACCCAC GCTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC CCTCCCCTCC CTGGCTGCTG TGAGACACCC 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCGGAAGCA GTTAGGAGAA 
AGTGTCACCA GTGTTCATCC TGCTTCCCCA GCCACACTGC CAACCAACCA CACAGAAGCC 
AGATCTTCCC ACTACCTCAA CCCTCCCCAG CCCCTCTGTC CTCCAAGGGG CACCACGGAC 
CTGGGCAGAA TCCTGGAGCC TGGGTACCTG GGTAGCAGTG GCCAGTGGGA CGTGATGAGG 
CCTCAGAAAG GGAGCATATC CGGGGACCTA TCCTCAGGCT CCTCCATGTA CCAGCTTAAC 
TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG GAGCATCTTG GTGAAATCCG GAACCTACGC 
CAGCGCCTGG AGGAGTCCAT CTGCATTAAT GACCGCCTGC GGGAGCAGCT GGAAAACAGG 
CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA ACCTCTAACT TCTACAGTCC AGGCCTGGAG 
TCCACACCTC AGCTCTCCAA TGAGAACAGA GTCCTTAGGG AAGAAAACCA CAGGCTTCAG 
GCTCAGCTCA GTCATGTTTC CAGAGAGCAC TCCCAGGAAA CAGAATGCCT GAGGGAGGCT 
CTGCTGACCT CTCGATCCCG GCTTCAAGAG CTGGAAATGG AGCTGGAGCA CCAAAAGGTA 
GACCGGCAGC AGTTACTGGA AGACCTGAAG GAGAAGCAGC AGGAGATCTT GCATTTCCAG 
GAGGAACGAC TGTCTCTCCA GGAGAAAGAC TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG 
CAGCAACAGT GCGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCAC TTTATGGTGA CTCCAAGAAG ACGCTGAAAG CTTATACCTG GGATGCCTGT 
CACCAAGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC AACTGTCTGC GATTGCAGCT CGAACAGCAG 
TTGGATGGTG GTGCCAGCAA AGCTGGCCTC AGCTCCTCCT CCGTTAACCA GAAATTCCCC 
ACCAACACTG ACCCTGGAAA CAAGCAGCCA TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA 
GTTCGGGATG TGGGCATGAA TTCTCCAGTT CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT 
CCTGGCCCAG AGACCACAAC CTTCAGTAGG ACGAACGAGC TGGGTTTGGA TGCTTCTCCA 
GTAATGAAGA ACCCTCGCAA GCTGGAGGGC GACGCTACTG ATGGCTCCTT TGCCAATAAG 
CATGGCCGCC ACGTCATTGG CCACATTGAT GACTATAGTG CCCTAAGAGA GCAGATTGGG 
GAGGGCAGAC TGCTGGTCAA GAAGATAGCA TCACTGGTGA GACCAACCTG CAGCTTCTCT 
GGTCTTGAAG CTCCAGGCAT AGAGCCACAG GGATGCCCAT GGCTACTGCC CCTTTCCCAG 
GTGATGGGCA GCGAAGGCAT CCAGGAGCTG AGGAGCAGCG CCACCGCCCT GCACCACCGG 
CTGGAGGAGT CGGCCTCCCT CCTCACCATG TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC 
GGCCCTGTGC TTGTGGACAA AGTGGGAGAG TCTATGAAAA AGGAGCTTCT GGAACTGAGA 
ACCAAACTAT CCAAACAGGA AAGTCTCCTT CAGAGTACAT CTGAGCGTCT GAAGACCGCC 
AACCAGCAGA AGGAGAGCAT GGAGCAGTTC ATCTTCAGCC AGTTGACCAG GACACACGAC 
GTTTTGAAGA AGGCAAGGAC TAATTTGGAG AAGACCTCTT ACAAGACTGC TTCTGTAAAG 
CCTTATTCCT GTCCCAGCAA AGGTGAAATC CCTAAGGGCC CTGCCATGCA CTCCAGTCCT 
GTGACCCTTG CCTTCCAGGA GACATGCAAG AAGCGAAGCC ACCAGAGGTC CTTAAAACAG 
CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204058.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204115.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X21, mRNA.

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CloneID	OBa17544
Clone ID Related Accession (Same CDS sequence)	XM_005204058.3 , XM_010826853.2 , XM_005204058.4
Accession Version	XM_005204058.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204058.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CGTGGACACG AGATTATTTT GCAGAAGATG ATGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACCAAAGATC GAGGCCCTCC TCTGCAGTCA 
CAGACTTGGA GAAGTGGTGA CAAGATCCCC TTGGGGCATT CCTTGAGAGC ATTTGAAAGA 
CCTCTTCAGG TGCAAACCCA GGCCCTTAGA GACTTTGAGA AGCACCTCAA TGACCTGAAG 
AAGGAGAACT TCAGCCTCAA GCTGCGCATC TACTTCCTGG AGGAGCGCAT GCAGCAGAAG 
TATGAGGCCA GCCGGGATGA CATCTACAAG CGGAACATCG AGCTGAAGGT TGAAGTAGAG 
AGCCTGAAAC GAGAACTCCA GGACAAGAGA CAGCATCCGG ATAAAACATG CGCTGATGCA 
GAGAATATCA ACAGCCATAA TGAAGCTGAG CTCCGGCGCC AGTTTGAGGA GAGACAGCAG 
GAGACGGAGC ATGTTTACGA GCTCCTGGAG AATAAGATCC AGCTTCTGCA AGAGGAGTCC 
AGACTAGCAA AAAATGAAGC CGCACGGATG GCGGCTCTGG TGGAAGCAGA GAAAGAGTGT 
AACCTGGAGC TCTCAGAGAA ACTGAAGGGA GTCACCAAGG AGAGGGAAGA TGTACCAGGA 
GACCGGGTCA AATCTGACCA GTACACTGAG GCCCTGGCCC AGAGGGACAA AAAAATTGAA 
GAACTGAGTC AAAGCCTGGC TGCACAGGAG AAACTTGTAG AGCAGCTGTC TCAAGAGAAA 
CAACAACTGC TACATCTGTT GGAGGAGCCA GCTGACATGG AAGTGCAGCC CATGACTGAA 
GACTTACTCA AACAACAGAA GTTGAATTCA AATGAGACCG GTACAACTCA GCAGTCTGTA 
TCTGATTCCC ATCTGGCAGA ACTCCAGGAA AAAATCCAGC AAACAGAGAC CACCAACAAG 
ATGCTTCAAG AAAAACTGAA TGAAATGAGC TATGAACTGA AGTCTGCTCA GGAGTCATCT 
CAGAAGCAAG ATGATACAAT TCAAAACCTC AAGGAAACTC TGAAAAGCAG GGAAAGTGAG 
ACCGAGGAGT TGTACCAGGT GATTGAAGGT CAAAACGACA CAATGGCTAA GCTTCGAGAA 
ATGCTGCACC AGAGTCAGCT TAAACAGCTT CATAACTCAG AGGGTACTTC CCCAGCTCAG 
CAACAGGTGG CTCTGCTTGA TCTTCAAAGT GCTTTATTCT GCAGCCAGCT TGAAATACAG 
AAGCTCCAGA GGGCAGTACG CCAAAAAGAA AGGCAGCTGG CTGATGCTAG ACGATGTGTG 
CGGTTTGTAG AGGCTGCAGC ACAGGAGAGA GAACAGCAGA AAGAGGCTTC TTGGAAACAT 
AACCAAGAAT TGCAAAAAGC CCTACAGCAG CTACAAGGAG AACTTCAGAG TAAGAGCCAA 
CAGCTTCATA CCCTGGAGGC TGAAAAATAT AATGAGATTC GCACCCAAGA GCAGCACATT 
CAGCACCTCC ACTACAGTCT GAGTCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTACC GAAATAACTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTAGGCAAA GAATACAAGA TAGAGATGTT GCCCTAGAGC GGGCTATAGA TGAAAAGTTC 
TCCAGCCTAG AAGAAAAGGA AAAAGAACTG CGTCAGCTTC ACCTTGCCAT GAGAGAGCGA 
GATCACGACT TAGAGAGGCT GCGCGGTATC CTCTCCGCCA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGTC CAAAGGCCTG GAAGTGGAAC AGTTGTCTGC CACCTGTCAG 
AACCTCCAGT GGCTGAAAGA AGAAATGGAA ACCAAATTTA GCCGTTGGCA GAAGGAGCAA 
GAAAGCATCA TTCAGCAGCT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTTACCGCAA CATTGCTCTG CAAACTTGGA CCAGGGCAGA CTGAGATAGT TGAGGAGCTG 
TGCCAGCGTC TACAGCAGAA GGAAAGGATG CTGCAGGACC TTCTGAGTGA TCGGAACAAG 
CAAGCAGTGG AATATGAAAT GGAGATTCAG GGCCTGCTTC AGTCCATGAG CACCAGAGAG 
CAGGAGAGCC ACGCTGCTGC AGAAAAGATG GTACAAGCTC TAATGGAAAG AAATTCAGAA 
TTACAGGCCC TGCGCCAATA TTTAGGAGGG AAAGATTTCA TGATGTCCCA AGCACCCCTC 
TCTAACCGAC CAGCTGAAGT TACCTCCATC AGCCCCCATC TTGGAGAGCA AACTGATCAA 
GGTTCAGTGC ATATACCCTC CAGAGATGAT AGCACTTCAT TGACTGCCAA AGGGGATGCT 
AGCGTACCCA GGTCCTCGTT AGGGGAGGTG GATACAGTTG CGGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAACT TGAGCTCATG GCTAAAAAAG AGAGAGAAAG TCGGATGGAA 
CTGTCTGCAC TCCAGTCCAT GGTGGCTGTG CAGGAGCAAG AGCTGCAGGT TCAGGCTGCT 
GACATGGAGT CCCTGACCAG GACCATACAG ATTAAGGAAG ACCTCATAAA GGACCTGCAG 
ATGCAGCTGG TTGATCCTGA AGACATCCCA GCTATGGAAC GCCTGACCCA GGAAGTCTTG 
CTTCTTCGGG AAAAAGTGGC TTCAGTAGAA TCACAAGGAC AGGAAACGTC TGGAAACCGA 
AGACAACAAT TGCTGCTGAT GCTCGAAGGA CTGGTAGATG AGCGGAGTCG GCTCAATGAG 
GCCTTACAGG CAGAGAGACA GCTCTATAGC AGTCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGCAAT 
CGGCTAGAAG AAGTTCTCGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCC 
GCCATTGGAG GTACAGCTGC AGGGGACGAC ACGGAGGATG CGAGCACGGA GTTTACCGAC 
AGTATCGAGG AGGAGGCTGC ACACAACAGC CACCAGCAAC TCATCAAGGT GGCGTTGGAG 
AAAAGCCGGG CAGCTGTGGA GACCCAGAAT GTGTCTCCTG CTCCTCCCAC CCTGACAGGA 
GGGGACGGTA ACAGGGGTCT TCAGGAGGAA ATGCTCCACC TGAGGGCTGA AATTCACCGG 
CACTTAGAGG AGAAGAGGAA AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATTGAGGAA 
GACAAGGAGA AGGGCGAGGC GATGGTGGAG ACTGTGGTTG CCAAAGGGGT TCTGAATGAG 
AATAGCCTTC AGGCTGAATT CAGAAAGGTC CAGGGAAAAC TGAAGAATGC CCGCAATATC 
ATCAGCCTCC TCAAAGAACA GCTGGTGTTG AGCAGCAAGG AAGGGACTAG TAAGCTTAAT 
CCTGAGCTCC TTGTGCACCT GGCCAAGGAG ATCAACAGAA TGAACACAGA GCTGGTTTAC 
TCTCCTGGAA AGCACCAATG CCTAGAAGAG GAGGGTGTGA CCACAAGGCC GTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTGCCCTC ACAGTGGATG CTCACCAACT GGATAACCAG 
CCCCAGACCC ATGACCCTGG GCCGCAGTCA GAATTTAGCT TCTCAGGATC CACGAAGCAC 
CTGCGCTCCC AGTTGGCACA ATGCAAACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCAGAAG CCACTGTCTT TGCCCAGGCC AACCAGCTGG AGAAATACAG AGTCATGTTT 
ACAGGTGGAT CGTCGGTGAA GCAGGACAGC AAGCAGGTGC AGGTGGACCT CCAGGACCTG 
GGCTATGAGA CCTGTGGCCG AAGCGAGAAC GAGGCTGAGC GGGAGGAGAG CACCAGCCCT 
GAGTGTGAGG AGCACGACAG CCTCAGGGAA ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG 
CAGGGACACC TGGACTCCAC AATGACCAGC CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA 
CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT GGAAAGTCAG AAGACATCTC TGTCCTACGT 
AAGGACATCA AAGATCTGAA GGCCCAGCTG CAGAACGCCA ACAAGGTCAT TCAGAACCTG 
AAGAGCCGGG TTCGGTCCCT CTCAGTTACA AGCGATTATT CATCTAGTCT GGAGAGACCC 
CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA GGAACTTCAC CGCACAGTGT CACTGATGAG 
GACGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTACC CTCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGGGTG TCCCAGCTAG AAGCCCAGCT CCCAAAAACT 
GGAACGGAAG GGAAGCTGGC CGAGGAGCTG AGATCTGCTT CGTGGCCTGG GAAATATGAT 
TCCCTGATTC AGGACCAGGC CCGAGAACTG TCCTACCTAC GTCAGAAAAT ACGGGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAC TCGAAAGACA CAGTAAAGTC TTTCGAGGAC 
CTCCTAAGGA GCAATGACAT TGACTACTAC CTGGGACAGA GCTTCCGGGA GCACCTCGCC 
CAGGGCAGCC AGCTGACAGA AAGGCTCACC AGCAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT CGAGCCACTG GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATCGAAGT CCTGCAGGCC AAGCTGGACG CCCGCTCCCT CACACCGTGC 
AGCAGCCATG CCTTGTCAGA CTCTCAGCGC TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC 
GAGCTGGAAG CCTGCTCTGA CATGGACATC GCCAGCGAGT ACACACACTA TGAAGAGAAG 
AAGGCTTCAC CTGGTCACTC AGATTCCATC CATCATTCGA GTCATTCTGC TGTACTGTCT 
TCCAAACCAT CAGCAACCAG TTCATCTCAG GAGGTTAAGG CCGAACCCAG CAGCAACCCC 
GTCAGCTTCC CAGCTCCCCA GAATCCCCCC AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTCCAAT CCATACCCAC GCTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC CCTCCCCTCC CTGGCTGCTG TGAGACACCC 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCGGAAGCA GTTAGGAGAA 
AGTGTCACCA GTGTTCATCC TGCTTCCCCA GCCACACTGC CAACCAACCA CACAGAAGCC 
AGATCTTCCC ACTACCTCAA CCCTCCCCAG CCCCTCTGTC CTCCAAGGGG CACCACGGAC 
CTGGGCAGAA TCCTGGAGCC TGGGTACCTG GGTAGCAGTG GCCAGTGGGA CGTGATGAGG 
CCTCAGAAAG GGAGCATATC CGGGGACCTA TCCTCAGGCT CCTCCATGTA CCAGCTTAAC 
TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG GAGCATCTTG GTGAAATCCG GAACCTACGC 
CAGCGCCTGG AGGAGTCCAT CTGCATTAAT GACCGCCTGC GGGAGCAGCT GGAAAACAGG 
CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA ACCTCTAACT TCTACAGTCC AGGCCTGGAG 
TCCACACCTC AGCTCTCCAA TGAGAACAGA GTCCTTAGGG AAGAAAACCA CAGGCTTCAG 
GCTCAGCTCA GTCATGTTTC CAGAGAGCAC TCCCAGGAAA CAGAATGCCT GAGGGAGGCT 
CTGCTGACCT CTCGATCCCG GCTTCAAGAG CTGGAAATGG AGCTGGAGCA CCAAAAGGTA 
GACCGGCAGC AGTTACTGGA AGACCTGAAG GAGAAGCAGC AGGAGATCTT GCATTTCCAG 
GAGGAACGAC TGTCTCTCCA GGAGAAAGAC TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG 
CAGCAACAGT GCGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCAC TTTATGGTGA CTCCAAGAAG ACGCTGAAAG CTTATACCTG GGATGCCTGT 
CACCAAGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC AACTGTCTGC GATTGCAGCT CGAACAGCAG 
TTGGATGGTG GTGCCAGCAA AGCTGGCCTC AGCTCCTCCT CCGTTAACCA GAAATTCCCC 
ACCAACACTG ACCCTGGAAA CAAGCAGCCA TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA 
GTTCGGGATG TGGGCATGAA TTCTCCAGTT CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT 
CCTGGCCCAG AGACCACAAC CTTCAGTAGG ACGAACGAGC TGGGTTTGGA TGCTTCTCCA 
GTAATGAAGA ACCCTCGCAA GCTGGAGGGC GACGCTACTG ATGGCTCCTT TGCCAATAAG 
CATGGCCGCC ACGTCATTGG CCACATTGAT GACTATAGTG CCCTAAGAGA GCAGATTGGG 
GAGGGCAGAC TGCTGGTCAA GAAGATAGCA TCACTGGTGA GACCAACCTG CAGCTTCTCT 
GGTCTTGAAG CTCCAGGCAT AGAGCCACAG GGATGCCCAT GGCTACTGCC CCTTTCCCAG 
GTGATGGGCA GCGAAGGCAT CCAGGAGCTG AGGAGCAGCG CCACCGCCCT GCACCACCGG 
CTGGAGGAGT CGGCCTCCCT CCTCACCATG TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC 
GGCCCTGTGC TTGTGGACAA AGTGGGAGAG TCTATGAAAA AGGAGCTTCT GGAACTGAGA 
ACCAAACTAT CCAAACAGGA AAGTCTCCTT CAGAGTACAT CTGAGCGTCT GAAGACCGCC 
AACCAGCAGA AGGAGAGCAT GGAGCAGTTC ATCTTCAGCC AGTTGACCAG GACACACGAC 
GTTTTGAAGA AGGCAAGGAC TAATTTGGAG AAGACCTCTT ACAAGACTGC TTCTGTAAAG 
CCTTATTCCT GTCCCAGCAA AGGTGAAATC CCTAAGGGCC CTGCCATGCA CTCCAGTCCT 
GTGACCCTTG CCTTCCAGGA GACATGCAAG AAGCGAAGCC ACCAGAGGTC CTTAAAACAG 
CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204115.1
CDS	309..7736
Translation	MEQAWTRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204058.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204058.4

ORF Insert Sequence:

ATGGAGCAGG CGTGGACACG AGATTATTTT GCAGAAGATG ATGGGGAGAT GGTACCCAGA 
ACAAGTCATG CAGCAGCTTT TCTTAGTGAC ACCAAAGATC GAGGCCCTCC TCTGCAGTCA 
CAGACTTGGA GAAGTGGTGA CAAGATCCCC TTGGGGCATT CCTTGAGAGC ATTTGAAAGA 
CCTCTTCAGG TGCAAACCCA GGCCCTTAGA GACTTTGAGA AGCACCTCAA TGACCTGAAG 
AAGGAGAACT TCAGCCTCAA GCTGCGCATC TACTTCCTGG AGGAGCGCAT GCAGCAGAAG 
TATGAGGCCA GCCGGGATGA CATCTACAAG CGGAACATCG AGCTGAAGGT TGAAGTAGAG 
AGCCTGAAAC GAGAACTCCA GGACAAGAGA CAGCATCCGG ATAAAACATG CGCTGATGCA 
GAGAATATCA ACAGCCATAA TGAAGCTGAG CTCCGGCGCC AGTTTGAGGA GAGACAGCAG 
GAGACGGAGC ATGTTTACGA GCTCCTGGAG AATAAGATCC AGCTTCTGCA AGAGGAGTCC 
AGACTAGCAA AAAATGAAGC CGCACGGATG GCGGCTCTGG TGGAAGCAGA GAAAGAGTGT 
AACCTGGAGC TCTCAGAGAA ACTGAAGGGA GTCACCAAGG AGAGGGAAGA TGTACCAGGA 
GACCGGGTCA AATCTGACCA GTACACTGAG GCCCTGGCCC AGAGGGACAA AAAAATTGAA 
GAACTGAGTC AAAGCCTGGC TGCACAGGAG AAACTTGTAG AGCAGCTGTC TCAAGAGAAA 
CAACAACTGC TACATCTGTT GGAGGAGCCA GCTGACATGG AAGTGCAGCC CATGACTGAA 
GACTTACTCA AACAACAGAA GTTGAATTCA AATGAGACCG GTACAACTCA GCAGTCTGTA 
TCTGATTCCC ATCTGGCAGA ACTCCAGGAA AAAATCCAGC AAACAGAGAC CACCAACAAG 
ATGCTTCAAG AAAAACTGAA TGAAATGAGC TATGAACTGA AGTCTGCTCA GGAGTCATCT 
CAGAAGCAAG ATGATACAAT TCAAAACCTC AAGGAAACTC TGAAAAGCAG GGAAAGTGAG 
ACCGAGGAGT TGTACCAGGT GATTGAAGGT CAAAACGACA CAATGGCTAA GCTTCGAGAA 
ATGCTGCACC AGAGTCAGCT TAAACAGCTT CATAACTCAG AGGGTACTTC CCCAGCTCAG 
CAACAGGTGG CTCTGCTTGA TCTTCAAAGT GCTTTATTCT GCAGCCAGCT TGAAATACAG 
AAGCTCCAGA GGGCAGTACG CCAAAAAGAA AGGCAGCTGG CTGATGCTAG ACGATGTGTG 
CGGTTTGTAG AGGCTGCAGC ACAGGAGAGA GAACAGCAGA AAGAGGCTTC TTGGAAACAT 
AACCAAGAAT TGCAAAAAGC CCTACAGCAG CTACAAGGAG AACTTCAGAG TAAGAGCCAA 
CAGCTTCATA CCCTGGAGGC TGAAAAATAT AATGAGATTC GCACCCAAGA GCAGCACATT 
CAGCACCTCC ACTACAGTCT GAGTCACAAA GAGCAGCTGC TTCAGGAATT TCGGGAGCTC 
CTGCAGTACC GAAATAACTC AGACAAAACC CTTGAAGCAA ATGAAATGTT GCTTGAGAAA 
CTTAGGCAAA GAATACAAGA TAGAGATGTT GCCCTAGAGC GGGCTATAGA TGAAAAGTTC 
TCCAGCCTAG AAGAAAAGGA AAAAGAACTG CGTCAGCTTC ACCTTGCCAT GAGAGAGCGA 
GATCACGACT TAGAGAGGCT GCGCGGTATC CTCTCCGCCA ATGAAGCTAC CATGCAGAGT 
ATGGAGAGTC TCCTGAGGTC CAAAGGCCTG GAAGTGGAAC AGTTGTCTGC CACCTGTCAG 
AACCTCCAGT GGCTGAAAGA AGAAATGGAA ACCAAATTTA GCCGTTGGCA GAAGGAGCAA 
GAAAGCATCA TTCAGCAGCT ACAGACATCT CTGCATGACA GGAACAAAGA AGTGGAGGAT 
CTTACCGCAA CATTGCTCTG CAAACTTGGA CCAGGGCAGA CTGAGATAGT TGAGGAGCTG 
TGCCAGCGTC TACAGCAGAA GGAAAGGATG CTGCAGGACC TTCTGAGTGA TCGGAACAAG 
CAAGCAGTGG AATATGAAAT GGAGATTCAG GGCCTGCTTC AGTCCATGAG CACCAGAGAG 
CAGGAGAGCC ACGCTGCTGC AGAAAAGATG GTACAAGCTC TAATGGAAAG AAATTCAGAA 
TTACAGGCCC TGCGCCAATA TTTAGGAGGG AAAGATTTCA TGATGTCCCA AGCACCCCTC 
TCTAACCGAC CAGCTGAAGT TACCTCCATC AGCCCCCATC TTGGAGAGCA AACTGATCAA 
GGTTCAGTGC ATATACCCTC CAGAGATGAT AGCACTTCAT TGACTGCCAA AGGGGATGCT 
AGCGTACCCA GGTCCTCGTT AGGGGAGGTG GATACAGTTG CGGGGCTGGA AAAAGAACTG 
AGTAATGCCA AAGAGGAACT TGAGCTCATG GCTAAAAAAG AGAGAGAAAG TCGGATGGAA 
CTGTCTGCAC TCCAGTCCAT GGTGGCTGTG CAGGAGCAAG AGCTGCAGGT TCAGGCTGCT 
GACATGGAGT CCCTGACCAG GACCATACAG ATTAAGGAAG ACCTCATAAA GGACCTGCAG 
ATGCAGCTGG TTGATCCTGA AGACATCCCA GCTATGGAAC GCCTGACCCA GGAAGTCTTG 
CTTCTTCGGG AAAAAGTGGC TTCAGTAGAA TCACAAGGAC AGGAAACGTC TGGAAACCGA 
AGACAACAAT TGCTGCTGAT GCTCGAAGGA CTGGTAGATG AGCGGAGTCG GCTCAATGAG 
GCCTTACAGG CAGAGAGACA GCTCTATAGC AGTCTGGTGA AGTTCCATGC CCATCCAGAG 
AGCTCTGAGA GAGACCGAAC TCTGCAGGTG GAACTAGAAG GGGCCCAGGT GATACGCAAT 
CGGCTAGAAG AAGTTCTCGG AAGAAGCCTG GAGCGCTTAA GCAGGCTGGA GACCCTGGCC 
GCCATTGGAG GTACAGCTGC AGGGGACGAC ACGGAGGATG CGAGCACGGA GTTTACCGAC 
AGTATCGAGG AGGAGGCTGC ACACAACAGC CACCAGCAAC TCATCAAGGT GGCGTTGGAG 
AAAAGCCGGG CAGCTGTGGA GACCCAGAAT GTGTCTCCTG CTCCTCCCAC CCTGACAGGA 
GGGGACGGTA ACAGGGGTCT TCAGGAGGAA ATGCTCCACC TGAGGGCTGA AATTCACCGG 
CACTTAGAGG AGAAGAGGAA AGCTGAGGGG GAACTGAAGG AGCTAAAGGC TCAAATTGAG 
GAAGCAGGAT TCTCCTCTGT GGCCCACATC AGGAACACCA TGCTGAGCCT TTGCCTTGAG 
AATGCAGAGC TGAAAGAGCA GATGGGAGAA GCAATGTCTG ATGGATGGGA GATTGAGGAA 
GACAAGGAGA AGGGCGAGGC GATGGTGGAG ACTGTGGTTG CCAAAGGGGT TCTGAATGAG 
AATAGCCTTC AGGCTGAATT CAGAAAGGTC CAGGGAAAAC TGAAGAATGC CCGCAATATC 
ATCAGCCTCC TCAAAGAACA GCTGGTGTTG AGCAGCAAGG AAGGGACTAG TAAGCTTAAT 
CCTGAGCTCC TTGTGCACCT GGCCAAGGAG ATCAACAGAA TGAACACAGA GCTGGTTTAC 
TCTCCTGGAA AGCACCAATG CCTAGAAGAG GAGGGTGTGA CCACAAGGCC GTGCCCCAGA 
CCCCAGAGCC TTGACCTTGG GGCTGCCCTC ACAGTGGATG CTCACCAACT GGATAACCAG 
CCCCAGACCC ATGACCCTGG GCCGCAGTCA GAATTTAGCT TCTCAGGATC CACGAAGCAC 
CTGCGCTCCC AGTTGGCACA ATGCAAACAA CGCTATCAGG ATCTCCAGGA GAAGCTGCTG 
ATATCAGAAG CCACTGTCTT TGCCCAGGCC AACCAGCTGG AGAAATACAG AGTCATGTTT 
ACAGGTGGAT CGTCGGTGAA GCAGGACAGC AAGCAGGTGC AGGTGGACCT CCAGGACCTG 
GGCTATGAGA CCTGTGGCCG AAGCGAGAAC GAGGCTGAGC GGGAGGAGAG CACCAGCCCT 
GAGTGTGAGG AGCACGACAG CCTCAGGGAA ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG 
CAGGGACACC TGGACTCCAC AATGACCAGC CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA 
CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT GGAAAGTCAG AAGACATCTC TGTCCTACGT 
AAGGACATCA AAGATCTGAA GGCCCAGCTG CAGAACGCCA ACAAGGTCAT TCAGAACCTG 
AAGAGCCGGG TTCGGTCCCT CTCAGTTACA AGCGATTATT CATCTAGTCT GGAGAGACCC 
CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA GGAACTTCAC CGCACAGTGT CACTGATGAG 
GACGAGGGGT GGCTGTCTGA TGGCACTGGG GCTTTCTACC CTCCAGGGCT TCAGGCTAAA 
AAGGATCTGG AGAGTCTGAT CCAGAGGGTG TCCCAGCTAG AAGCCCAGCT CCCAAAAACT 
GGAACGGAAG GGAAGCTGGC CGAGGAGCTG AGATCTGCTT CGTGGCCTGG GAAATATGAT 
TCCCTGATTC AGGACCAGGC CCGAGAACTG TCCTACCTAC GTCAGAAAAT ACGGGAAGGG 
AGAGGTATCT GTTATCTTCT CACCCAGCAC TCGAAAGACA CAGTAAAGTC TTTCGAGGAC 
CTCCTAAGGA GCAATGACAT TGACTACTAC CTGGGACAGA GCTTCCGGGA GCACCTCGCC 
CAGGGCAGCC AGCTGACAGA AAGGCTCACC AGCAAACTCA GCACCAAGGA TCATAAAAGT 
GAGAAAGATC AAGCTGGACT CGAGCCACTG GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG 
AAGGAGAAAG TGATCGAAGT CCTGCAGGCC AAGCTGGACG CCCGCTCCCT CACACCGTGC 
AGCAGCCATG CCTTGTCAGA CTCTCAGCGC TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC 
GAGCTGGAAG CCTGCTCTGA CATGGACATC GCCAGCGAGT ACACACACTA TGAAGAGAAG 
AAGGCTTCAC CTGGTCACTC AGATTCCATC CATCATTCGA GTCATTCTGC TGTACTGTCT 
TCCAAACCAT CAGCAACCAG TTCATCTCAG GAGGTTAAGG CCGAACCCAG CAGCAACCCC 
GTCAGCTTCC CAGCTCCCCA GAATCCCCCC AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT 
CATTTCCAAT CCATACCCAC GCTGGTTAGC CTTCCCCAGG CACCACTGCC CTCAGCTCCA 
CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC CCTCCCCTCC CTGGCTGCTG TGAGACACCC 
ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG CTGCAGATGC TGCGGAAGCA GTTAGGAGAA 
AGTGTCACCA GTGTTCATCC TGCTTCCCCA GCCACACTGC CAACCAACCA CACAGAAGCC 
AGATCTTCCC ACTACCTCAA CCCTCCCCAG CCCCTCTGTC CTCCAAGGGG CACCACGGAC 
CTGGGCAGAA TCCTGGAGCC TGGGTACCTG GGTAGCAGTG GCCAGTGGGA CGTGATGAGG 
CCTCAGAAAG GGAGCATATC CGGGGACCTA TCCTCAGGCT CCTCCATGTA CCAGCTTAAC 
TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG GAGCATCTTG GTGAAATCCG GAACCTACGC 
CAGCGCCTGG AGGAGTCCAT CTGCATTAAT GACCGCCTGC GGGAGCAGCT GGAAAACAGG 
CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA ACCTCTAACT TCTACAGTCC AGGCCTGGAG 
TCCACACCTC AGCTCTCCAA TGAGAACAGA GTCCTTAGGG AAGAAAACCA CAGGCTTCAG 
GCTCAGCTCA GTCATGTTTC CAGAGAGCAC TCCCAGGAAA CAGAATGCCT GAGGGAGGCT 
CTGCTGACCT CTCGATCCCG GCTTCAAGAG CTGGAAATGG AGCTGGAGCA CCAAAAGGTA 
GACCGGCAGC AGTTACTGGA AGACCTGAAG GAGAAGCAGC AGGAGATCTT GCATTTCCAG 
GAGGAACGAC TGTCTCTCCA GGAGAAAGAC TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG 
CAGCAACAGT GCGAAGAGAA ACAGCAGCTC TTCCAGTCCC TCCAGTCAGA GCTACAGATC 
TATGAGGCAC TTTATGGTGA CTCCAAGAAG ACGCTGAAAG CTTATACCTG GGATGCCTGT 
CACCAAGTCC CTTTGAGCAG TGACTTGAGC CACCTGGTGG CAGAGATCCG AGCTTTGCGA 
GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC AACTGTCTGC GATTGCAGCT CGAACAGCAG 
TTGGATGGTG GTGCCAGCAA AGCTGGCCTC AGCTCCTCCT CCGTTAACCA GAAATTCCCC 
ACCAACACTG ACCCTGGAAA CAAGCAGCCA TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA 
GTTCGGGATG TGGGCATGAA TTCTCCAGTT CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT 
CCTGGCCCAG AGACCACAAC CTTCAGTAGG ACGAACGAGC TGGGTTTGGA TGCTTCTCCA 
GTAATGAAGA ACCCTCGCAA GCTGGAGGGC GACGCTACTG ATGGCTCCTT TGCCAATAAG 
CATGGCCGCC ACGTCATTGG CCACATTGAT GACTATAGTG CCCTAAGAGA GCAGATTGGG 
GAGGGCAGAC TGCTGGTCAA GAAGATAGCA TCACTGGTGA GACCAACCTG CAGCTTCTCT 
GGTCTTGAAG CTCCAGGCAT AGAGCCACAG GGATGCCCAT GGCTACTGCC CCTTTCCCAG 
GTGATGGGCA GCGAAGGCAT CCAGGAGCTG AGGAGCAGCG CCACCGCCCT GCACCACCGG 
CTGGAGGAGT CGGCCTCCCT CCTCACCATG TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC 
GGCCCTGTGC TTGTGGACAA AGTGGGAGAG TCTATGAAAA AGGAGCTTCT GGAACTGAGA 
ACCAAACTAT CCAAACAGGA AAGTCTCCTT CAGAGTACAT CTGAGCGTCT GAAGACCGCC 
AACCAGCAGA AGGAGAGCAT GGAGCAGTTC ATCTTCAGCC AGTTGACCAG GACACACGAC 
GTTTTGAAGA AGGCAAGGAC TAATTTGGAG AAGACCTCTT ACAAGACTGC TTCTGTAAAG 
CCTTATTCCT GTCCCAGCAA AGGTGAAATC CCTAAGGGCC CTGCCATGCA CTCCAGTCCT 
GTGACCCTTG CCTTCCAGGA GACATGCAAG AAGCGAAGCC ACCAGAGGTC CTTAAAACAG 
CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803125.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801427.1 Bos taurus myomegalin (LOC508547), transcript variant X8, mRNA.

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CloneID	OBa47199
Clone ID Related Accession (Same CDS sequence)	XM_010803125.2 , XM_010803125.3 , XM_010826852.2
Accession Version	XM_010803125.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801427.1
CDS	433..7968
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010803125.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803125.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803125.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801427.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X12, mRNA.

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CloneID	OBa47199
Clone ID Related Accession (Same CDS sequence)	XM_010803125.2 , XM_010803125.3 , XM_010826852.2
Accession Version	XM_010803125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801427.1
CDS	714..8249
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010803125.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803125.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826852.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825154.1 Bos taurus myomegalin (LOC508547), transcript variant X8, mRNA.

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CloneID	OBa47199
Clone ID Related Accession (Same CDS sequence)	XM_010803125.2 , XM_010803125.3 , XM_010826852.2
Accession Version	XM_010826852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825154.1
CDS	433..7968
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010826852.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826852.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGCCACAGGG ATGCCCATGG CTACTGCCCC TTTCCCAGGT GATGGGCAGC 
GAAGGCATCC AGGAGCTGAG GAGCAGCGCC ACCGCCCTGC ACCACCGGCT GGAGGAGTCG 
GCCTCCCTCC TCACCATGTT CTGGCGAGCA GCCTTGCCAA GCTCCCCCGG CCCTGTGCTT 
GTGGACAAAG TGGGAGAGTC TATGAAAAAG GAGCTTCTGG AACTGAGAAC CAAACTATCC 
AAACAGGAAA GTCTCCTTCA GAGTACATCT GAGCGTCTGA AGACCGCCAA CCAGCAGAAG 
GAGAGCATGG AGCAGTTCAT CTTCAGCCAG TTGACCAGGA CACACGACGT TTTGAAGAAG 
GCAAGGACTA ATTTGGAGAA GACCTCTTAC AAGACTGCTT CTGTAAAGCC TTATTCCTGT 
CCCAGCAAAG GTGAAATCCC TAAGGGCCCT GCCATGCACT CCAGTCCTGT GACCCTTGCC 
TTCCAGGAGA CATGCAAGAA GCGAAGCCAC CAGAGGTCCT TAAAACAGCA GGAAGGATGG 
GCCTGTGCCC CTTCTTCACA GCTCCCTGTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803126.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801428.1 Bos taurus myomegalin (LOC508547), transcript variant X15, mRNA.

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CloneID	OBa47200
Clone ID Related Accession (Same CDS sequence)	XM_010803126.2 , XM_010803126.3 , XM_010826854.2
Accession Version	XM_010803126.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801428.1
CDS	434..7777
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_010803126.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803126.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803126.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801428.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X23, mRNA.

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CloneID	OBa47200
Clone ID Related Accession (Same CDS sequence)	XM_010803126.2 , XM_010803126.3 , XM_010826854.2
Accession Version	XM_010803126.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801428.1
CDS	714..8057
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_010803126.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803126.3

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826854.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825156.1 Bos taurus myomegalin (LOC508547), transcript variant X15, mRNA.

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CloneID	OBa47200
Clone ID Related Accession (Same CDS sequence)	XM_010803126.2 , XM_010803126.3 , XM_010826854.2
Accession Version	XM_010826854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825156.1
CDS	434..7777
Translation	MKEICRICARELCGNQRRWIFHTASKLNLQVLLSHVLGKDVSRDGKAEFACSKCAFMLDRIYRFDTVIARIEALSIERLQKLLLEKDRLKFCIASMYRKNNDDPGAETKAGNGTVDISGLPDVRYSALLQEDFAYSGFECWVENEDQIQEHSCHASEGPGNRPRRCRGCAALRVADSDYEAICKVPRKVAKSISCGPSTRWSTSICTEEPALSEVGPPDLPNAKVPPDGESMEEGTPGSSVESLDASIQASPPQPKDEEPERSAKELAKCDCCSDEQAPQHTCNHKLELALSMIKGLDYKPIQSPRGSKLPIPVKSNPSGTRPGHIMTDGVSSGFLNRSLKPLYKTPVSYPLEISDLQELWDDLCEDYLPLRVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_010826854.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826854.2

ORF Insert Sequence:

ATGAAGGAGA TTTGCAGGAT CTGTGCCCGG GAGCTGTGCG GAAACCAGCG GCGCTGGATC 
TTCCACACGG CGTCCAAGCT CAACCTCCAG GTTCTGTTGT CGCATGTCCT GGGCAAGGAT 
GTCTCCCGCG ATGGCAAAGC TGAGTTTGCT TGCAGCAAGT GCGCGTTCAT GCTCGATCGG 
ATCTATAGAT TCGACACCGT CATTGCCCGG ATCGAAGCCC TTTCTATTGA GCGCTTGCAG 
AAGCTGCTGC TGGAGAAGGA CCGCCTCAAG TTCTGCATTG CTAGCATGTA TCGGAAGAAT 
AACGATGACC CTGGCGCGGA GACCAAGGCG GGGAATGGAA CGGTTGACAT TTCCGGCTTA 
CCCGATGTCA GATACTCTGC ACTGCTCCAA GAAGACTTTG CCTATTCAGG GTTCGAGTGT 
TGGGTAGAAA ATGAGGATCA GATTCAGGAG CACAGCTGTC ATGCTTCGGA AGGCCCCGGA 
AATCGACCCA GGAGATGCCG AGGCTGTGCA GCTTTGCGGG TGGCTGATTC TGACTATGAA 
GCCATTTGTA AGGTACCTCG AAAGGTGGCG AAAAGTATCT CGTGTGGCCC CTCTACCAGA 
TGGTCCACCA GCATTTGCAC TGAAGAACCA GCGTTGTCCG AGGTTGGGCC ACCGGATTTA 
CCAAATGCGA AGGTACCGCC AGATGGAGAA AGCATGGAGG AAGGGACCCC GGGATCCTCT 
GTGGAATCTT TGGATGCAAG CATCCAGGCT AGTCCTCCAC AACCAAAGGA TGAGGAACCT 
GAGAGAAGTG CAAAGGAACT TGCAAAGTGT GACTGCTGTT CAGATGAGCA GGCTCCCCAG 
CATACGTGTA ACCATAAGCT GGAGCTAGCT CTTAGCATGA TTAAAGGTCT TGATTATAAG 
CCCATCCAGA GCCCCCGAGG GAGCAAGCTT CCTATTCCAG TGAAATCCAA TCCATCTGGA 
ACCAGGCCTG GCCATATCAT GACAGATGGA GTTAGTTCTG GTTTCCTTAA CAGGTCTTTG 
AAACCCCTTT ACAAGACACC TGTGAGTTAT CCCTTGGAGA TTTCAGACCT GCAGGAGCTG 
TGGGATGATC TCTGTGAAGA TTATTTGCCA CTTCGGGTCC AGCCCATGAC TGAAGACTTA 
CTCAAACAAC AGAAGTTGAA TTCAAATGAG ACCGGTACAA CTCAGCAGTC TGTATCTGAT 
TCCCATCTGG CAGAACTCCA GGAAAAAATC CAGCAAACAG AGACCACCAA CAAGATGCTT 
CAAGAAAAAC TGAATGAAAT GAGCTATGAA CTGAAGTCTG CTCAGGAGTC ATCTCAGAAG 
CAAGATGATA CAATTCAAAA CCTCAAGGAA ACTCTGAAAA GCAGGGAAAG TGAGACCGAG 
GAGTTGTACC AGGTGATTGA AGGTCAAAAC GACACAATGG CTAAGCTTCG AGAAATGCTG 
CACCAGAGTC AGCTTAAACA GCTTCATAAC TCAGAGGGTA CTTCCCCAGC TCAGCAACAG 
GTGGCTCTGC TTGATCTTCA AAGTGCTTTA TTCTGCAGCC AGCTTGAAAT ACAGAAGCTC 
CAGAGGGCAG TACGCCAAAA AGAAAGGCAG CTGGCTGATG CTAGACGATG TGTGCGGTTT 
GTAGAGGCTG CAGCACAGGA GAGAGAACAG CAGAAAGAGG CTTCTTGGAA ACATAACCAA 
GAATTGCAAA AAGCCCTACA GCAGCTACAA GGAGAACTTC AGAGTAAGAG CCAACAGCTT 
CATACCCTGG AGGCTGAAAA ATATAATGAG ATTCGCACCC AAGAGCAGCA CATTCAGCAC 
CTCCACTACA GTCTGAGTCA CAAAGAGCAG CTGCTTCAGG AATTTCGGGA GCTCCTGCAG 
TACCGAAATA ACTCAGACAA AACCCTTGAA GCAAATGAAA TGTTGCTTGA GAAACTTAGG 
CAAAGAATAC AAGATAGAGA TGTTGCCCTA GAGCGGGCTA TAGATGAAAA GTTCTCCAGC 
CTAGAAGAAA AGGAAAAAGA ACTGCGTCAG CTTCACCTTG CCATGAGAGA GCGAGATCAC 
GACTTAGAGA GGCTGCGCGG TATCCTCTCC GCCAATGAAG CTACCATGCA GAGTATGGAG 
AGTCTCCTGA GGTCCAAAGG CCTGGAAGTG GAACAGTTGT CTGCCACCTG TCAGAACCTC 
CAGTGGCTGA AAGAAGAAAT GGAAACCAAA TTTAGCCGTT GGCAGAAGGA GCAAGAAAGC 
ATCATTCAGC AGCTACAGAC ATCTCTGCAT GACAGGAACA AAGAAGTGGA GGATCTTACC 
GCAACATTGC TCTGCAAACT TGGACCAGGG CAGACTGAGA TAGTTGAGGA GCTGTGCCAG 
CGTCTACAGC AGAAGGAAAG GATGCTGCAG GACCTTCTGA GTGATCGGAA CAAGCAAGCA 
GTGGAATATG AAATGGAGAT TCAGGGCCTG CTTCAGTCCA TGAGCACCAG AGAGCAGGAG 
AGCCACGCTG CTGCAGAAAA GATGGTACAA GCTCTAATGG AAAGAAATTC AGAATTACAG 
GCCCTGCGCC AATATTTAGG AGGGAAAGAT TTCATGATGT CCCAAGCACC CCTCTCTAAC 
CGACCAGCTG AAGTTACCTC CATCAGCCCC CATCTTGGAG AGCAAACTGA TCAAGGTTCA 
GTGCATATAC CCTCCAGAGA TGATAGCACT TCATTGACTG CCAAAGGGGA TGCTAGCGTA 
CCCAGGTCCT CGTTAGGGGA GGTGGATACA GTTGCGGGGC TGGAAAAAGA ACTGAGTAAT 
GCCAAAGAGG AACTTGAGCT CATGGCTAAA AAAGAGAGAG AAAGTCGGAT GGAACTGTCT 
GCACTCCAGT CCATGGTGGC TGTGCAGGAG CAAGAGCTGC AGGTTCAGGC TGCTGACATG 
GAGTCCCTGA CCAGGACCAT ACAGATTAAG GAAGACCTCA TAAAGGACCT GCAGATGCAG 
CTGGTTGATC CTGAAGACAT CCCAGCTATG GAACGCCTGA CCCAGGAAGT CTTGCTTCTT 
CGGGAAAAAG TGGCTTCAGT AGAATCACAA GGACAGGAAA CGTCTGGAAA CCGAAGACAA 
CAATTGCTGC TGATGCTCGA AGGACTGGTA GATGAGCGGA GTCGGCTCAA TGAGGCCTTA 
CAGGCAGAGA GACAGCTCTA TAGCAGTCTG GTGAAGTTCC ATGCCCATCC AGAGAGCTCT 
GAGAGAGACC GAACTCTGCA GGTGGAACTA GAAGGGGCCC AGGTGATACG CAATCGGCTA 
GAAGAAGTTC TCGGAAGAAG CCTGGAGCGC TTAAGCAGGC TGGAGACCCT GGCCGCCATT 
GGAGGTACAG CTGCAGGGGA CGACACGGAG GATGCGAGCA CGGAGTTTAC CGACAGTATC 
GAGGAGGAGG CTGCACACAA CAGCCACCAG CAACTCATCA AGGTGGCGTT GGAGAAAAGC 
CGGGCAGCTG TGGAGACCCA GAATGTGTCT CCTGCTCCTC CCACCCTGAC AGGAGGGGAC 
GGTAACAGGG GTCTTCAGGA GGAAATGCTC CACCTGAGGG CTGAAATTCA CCGGCACTTA 
GAGGAGAAGA GGAAAGCTGA GGGGGAACTG AAGGAGCTAA AGGCTCAAAT TGAGGAAGCA 
GGATTCTCCT CTGTGGCCCA CATCAGGAAC ACCATGCTGA GCCTTTGCCT TGAGAATGCA 
GAGCTGAAAG AGCAGATGGG AGAAGCAATG TCTGATGGAT GGGAGATTGA GGAAGACAAG 
GAGAAGGGCG AGGCGATGGT GGAGACTGTG GTTGCCAAAG GGGTTCTGAA TGAGAATAGC 
CTTCAGGCTG AATTCAGAAA GGTCCAGGGA AAACTGAAGA ATGCCCGCAA TATCATCAGC 
CTCCTCAAAG AACAGCTGGT GTTGAGCAGC AAGGAAGGGA CTAGTAAGCT TAATCCTGAG 
CTCCTTGTGC ACCTGGCCAA GGAGATCAAC AGAATGAACA CAGAGCTGGT TTACTCTCCT 
GGAAAGCACC AATGCCTAGA AGAGGAGGGT GTGACCACAA GGCCGTGCCC CAGACCCCAG 
AGCCTTGACC TTGGGGCTGC CCTCACAGTG GATGCTCACC AACTGGATAA CCAGCCCCAG 
ACCCATGACC CTGGGCCGCA GTCAGAATTT AGCTTCTCAG GATCCACGAA GCACCTGCGC 
TCCCAGTTGG CACAATGCAA ACAACGCTAT CAGGATCTCC AGGAGAAGCT GCTGATATCA 
GAAGCCACTG TCTTTGCCCA GGCCAACCAG CTGGAGAAAT ACAGAGTCAT GTTTACAGGT 
GGATCGTCGG TGAAGCAGGA CAGCAAGCAG GTGCAGGTGG ACCTCCAGGA CCTGGGCTAT 
GAGACCTGTG GCCGAAGCGA GAACGAGGCT GAGCGGGAGG AGAGCACCAG CCCTGAGTGT 
GAGGAGCACG ACAGCCTCAG GGAAACAGTC TTGATGGAGG GGCTGTGCTC CAAGCAGGGA 
CACCTGGACT CCACAATGAC CAGCCCCCCT GGGAAGAAGC CCCTGGAGAG CCAACCAGGG 
AAGCAGGAGG AGCTCCGGGC GTATGGAAAG TCAGAAGACA TCTCTGTCCT ACGTAAGGAC 
ATCAAAGATC TGAAGGCCCA GCTGCAGAAC GCCAACAAGG TCATTCAGAA CCTGAAGAGC 
CGGGTTCGGT CCCTCTCAGT TACAAGCGAT TATTCATCTA GTCTGGAGAG ACCCCGAAAG 
CTGAAGGCCG TTGGTGCCCT AGAAGGAACT TCACCGCACA GTGTCACTGA TGAGGACGAG 
GGGTGGCTGT CTGATGGCAC TGGGGCTTTC TACCCTCCAG GGCTTCAGGC TAAAAAGGAT 
CTGGAGAGTC TGATCCAGAG GGTGTCCCAG CTAGAAGCCC AGCTCCCAAA AACTGGAACG 
GAAGGGAAGC TGGCCGAGGA GCTGAGATCT GCTTCGTGGC CTGGGAAATA TGATTCCCTG 
ATTCAGGACC AGGCCCGAGA ACTGTCCTAC CTACGTCAGA AAATACGGGA AGGGAGAGGT 
ATCTGTTATC TTCTCACCCA GCACTCGAAA GACACAGTAA AGTCTTTCGA GGACCTCCTA 
AGGAGCAATG ACATTGACTA CTACCTGGGA CAGAGCTTCC GGGAGCACCT CGCCCAGGGC 
AGCCAGCTGA CAGAAAGGCT CACCAGCAAA CTCAGCACCA AGGATCATAA AAGTGAGAAA 
GATCAAGCTG GACTCGAGCC ACTGGCTCTG AGGCTTAGCA GGGAGCTGCA GGAGAAGGAG 
AAAGTGATCG AAGTCCTGCA GGCCAAGCTG GACGCCCGCT CCCTCACACC GTGCAGCAGC 
CATGCCTTGT CAGACTCTCA GCGCTCTCCC AGCAGCTCCT CCTTCTTGTC TGACGAGCTG 
GAAGCCTGCT CTGACATGGA CATCGCCAGC GAGTACACAC ACTATGAAGA GAAGAAGGCT 
TCACCTGGTC ACTCAGATTC CATCCATCAT TCGAGTCATT CTGCTGTACT GTCTTCCAAA 
CCATCAGCAA CCAGTTCATC TCAGGAGGTT AAGGCCGAAC CCAGCAGCAA CCCCGTCAGC 
TTCCCAGCTC CCCAGAATCC CCCCAAGGAG GCCAGCCAGG CCCATCCAGG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGGTGAAATC CCTAAGGGCC 
CTGCCATGCA CTCCAGTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826855.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825157.1 Bos taurus myomegalin (LOC508547), transcript variant X18, mRNA.

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CloneID	OBa47201
Clone ID Related Accession (Same CDS sequence)	XM_010826855.2 , XM_010803128.2
Accession Version	XM_010826855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825157.1
CDS	171..7370
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010826855.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826855.2

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803128.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801430.1 Bos taurus myomegalin (LOC508547), transcript variant X18, mRNA.

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CloneID	OBa47201
Clone ID Related Accession (Same CDS sequence)	XM_010826855.2 , XM_010803128.2
Accession Version	XM_010803128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801430.1
CDS	171..7370
Translation	MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010803128.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803128.2

ORF Insert Sequence:

ATGTCTAATG GATATCGCAC TCTGTCCCAG CACCTCAATG ACCTGAAGAA GGAGAACTTC 
AGCCTCAAGC TGCGCATCTA CTTCCTGGAG GAGCGCATGC AGCAGAAGTA TGAGGCCAGC 
CGGGATGACA TCTACAAGCG GAACATCGAG CTGAAGGTTG AAGTAGAGAG CCTGAAACGA 
GAACTCCAGG ACAAGAGACA GCATCCGGAT AAAACATGCG CTGATGCAGA GAATATCAAC 
AGCCATAATG AAGCTGAGCT CCGGCGCCAG TTTGAGGAGA GACAGCAGGA GACGGAGCAT 
GTTTACGAGC TCCTGGAGAA TAAGATCCAG CTTCTGCAAG AGGAGTCCAG ACTAGCAAAA 
AATGAAGCCG CACGGATGGC GGCTCTGGTG GAAGCAGAGA AAGAGTGTAA CCTGGAGCTC 
TCAGAGAAAC TGAAGGGAGT CACCAAGGAG AGGGAAGATG TACCAGGAGA CCGGGTCAAA 
TCTGACCAGT ACACTGAGGC CCTGGCCCAG AGGGACAAAA AAATTGAAGA ACTGAGTCAA 
AGCCTGGCTG CACAGGAGAA ACTTGTAGAG CAGCTGTCTC AAGAGAAACA ACAACTGCTA 
CATCTGTTGG AGGAGCCAGC TGACATGGAA GTGCAGCCCA TGACTGAAGA CTTACTCAAA 
CAACAGAAGT TGAATTCAAA TGAGACCGGT ACAACTCAGC AGTCTGTATC TGATTCCCAT 
CTGGCAGAAC TCCAGGAAAA AATCCAGCAA ACAGAGACCA CCAACAAGAT GCTTCAAGAA 
AAACTGAATG AAATGAGCTA TGAACTGAAG TCTGCTCAGG AGTCATCTCA GAAGCAAGAT 
GATACAATTC AAAACCTCAA GGAAACTCTG AAAAGCAGGG AAAGTGAGAC CGAGGAGTTG 
TACCAGGTGA TTGAAGGTCA AAACGACACA ATGGCTAAGC TTCGAGAAAT GCTGCACCAG 
AGTCAGCTTA AACAGCTTCA TAACTCAGAG GGTACTTCCC CAGCTCAGCA ACAGGTGGCT 
CTGCTTGATC TTCAAAGTGC TTTATTCTGC AGCCAGCTTG AAATACAGAA GCTCCAGAGG 
GCAGTACGCC AAAAAGAAAG GCAGCTGGCT GATGCTAGAC GATGTGTGCG GTTTGTAGAG 
GCTGCAGCAC AGGAGAGAGA ACAGCAGAAA GAGGCTTCTT GGAAACATAA CCAAGAATTG 
CAAAAAGCCC TACAGCAGCT ACAAGGAGAA CTTCAGAGTA AGAGCCAACA GCTTCATACC 
CTGGAGGCTG AAAAATATAA TGAGATTCGC ACCCAAGAGC AGCACATTCA GCACCTCCAC 
TACAGTCTGA GTCACAAAGA GCAGCTGCTT CAGGAATTTC GGGAGCTCCT GCAGTACCGA 
AATAACTCAG ACAAAACCCT TGAAGCAAAT GAAATGTTGC TTGAGAAACT TAGGCAAAGA 
ATACAAGATA GAGATGTTGC CCTAGAGCGG GCTATAGATG AAAAGTTCTC CAGCCTAGAA 
GAAAAGGAAA AAGAACTGCG TCAGCTTCAC CTTGCCATGA GAGAGCGAGA TCACGACTTA 
GAGAGGCTGC GCGGTATCCT CTCCGCCAAT GAAGCTACCA TGCAGAGTAT GGAGAGTCTC 
CTGAGGTCCA AAGGCCTGGA AGTGGAACAG TTGTCTGCCA CCTGTCAGAA CCTCCAGTGG 
CTGAAAGAAG AAATGGAAAC CAAATTTAGC CGTTGGCAGA AGGAGCAAGA AAGCATCATT 
CAGCAGCTAC AGACATCTCT GCATGACAGG AACAAAGAAG TGGAGGATCT TACCGCAACA 
TTGCTCTGCA AACTTGGACC AGGGCAGACT GAGATAGTTG AGGAGCTGTG CCAGCGTCTA 
CAGCAGAAGG AAAGGATGCT GCAGGACCTT CTGAGTGATC GGAACAAGCA AGCAGTGGAA 
TATGAAATGG AGATTCAGGG CCTGCTTCAG TCCATGAGCA CCAGAGAGCA GGAGAGCCAC 
GCTGCTGCAG AAAAGATGGT ACAAGCTCTA ATGGAAAGAA ATTCAGAATT ACAGGCCCTG 
CGCCAATATT TAGGAGGGAA AGATTTCATG ATGTCCCAAG CACCCCTCTC TAACCGACCA 
GCTGAAGTTA CCTCCATCAG CCCCCATCTT GGAGAGCAAA CTGATCAAGG TTCAGTGCAT 
ATACCCTCCA GAGATGATAG CACTTCATTG ACTGCCAAAG GGGATGCTAG CGTACCCAGG 
TCCTCGTTAG GGGAGGTGGA TACAGTTGCG GGGCTGGAAA AAGAACTGAG TAATGCCAAA 
GAGGAACTTG AGCTCATGGC TAAAAAAGAG AGAGAAAGTC GGATGGAACT GTCTGCACTC 
CAGTCCATGG TGGCTGTGCA GGAGCAAGAG CTGCAGGTTC AGGCTGCTGA CATGGAGTCC 
CTGACCAGGA CCATACAGAT TAAGGAAGAC CTCATAAAGG ACCTGCAGAT GCAGCTGGTT 
GATCCTGAAG ACATCCCAGC TATGGAACGC CTGACCCAGG AAGTCTTGCT TCTTCGGGAA 
AAAGTGGCTT CAGTAGAATC ACAAGGACAG GAAACGTCTG GAAACCGAAG ACAACAATTG 
CTGCTGATGC TCGAAGGACT GGTAGATGAG CGGAGTCGGC TCAATGAGGC CTTACAGGCA 
GAGAGACAGC TCTATAGCAG TCTGGTGAAG TTCCATGCCC ATCCAGAGAG CTCTGAGAGA 
GACCGAACTC TGCAGGTGGA ACTAGAAGGG GCCCAGGTGA TACGCAATCG GCTAGAAGAA 
GTTCTCGGAA GAAGCCTGGA GCGCTTAAGC AGGCTGGAGA CCCTGGCCGC CATTGGAGGT 
ACAGCTGCAG GGGACGACAC GGAGGATGCG AGCACGGAGT TTACCGACAG TATCGAGGAG 
GAGGCTGCAC ACAACAGCCA CCAGCAACTC ATCAAGGTGG CGTTGGAGAA AAGCCGGGCA 
GCTGTGGAGA CCCAGAATGT GTCTCCTGCT CCTCCCACCC TGACAGGAGG GGACGGTAAC 
AGGGGTCTTC AGGAGGAAAT GCTCCACCTG AGGGCTGAAA TTCACCGGCA CTTAGAGGAG 
AAGAGGAAAG CTGAGGGGGA ACTGAAGGAG CTAAAGGCTC AAATTGAGGA AGCAGGATTC 
TCCTCTGTGG CCCACATCAG GAACACCATG CTGAGCCTTT GCCTTGAGAA TGCAGAGCTG 
AAAGAGCAGA TGGGAGAAGC AATGTCTGAT GGATGGGAGA TTGAGGAAGA CAAGGAGAAG 
GGCGAGGCGA TGGTGGAGAC TGTGGTTGCC AAAGGGGTTC TGAATGAGAA TAGCCTTCAG 
GCTGAATTCA GAAAGGTCCA GGGAAAACTG AAGAATGCCC GCAATATCAT CAGCCTCCTC 
AAAGAACAGC TGGTGTTGAG CAGCAAGGAA GGGACTAGTA AGCTTAATCC TGAGCTCCTT 
GTGCACCTGG CCAAGGAGAT CAACAGAATG AACACAGAGC TGGTTTACTC TCCTGGAAAG 
CACCAATGCC TAGAAGAGGA GGGTGTGACC ACAAGGCCGT GCCCCAGACC CCAGAGCCTT 
GACCTTGGGG CTGCCCTCAC AGTGGATGCT CACCAACTGG ATAACCAGCC CCAGACCCAT 
GACCCTGGGC CGCAGTCAGA ATTTAGCTTC TCAGGATCCA CGAAGCACCT GCGCTCCCAG 
TTGGCACAAT GCAAACAACG CTATCAGGAT CTCCAGGAGA AGCTGCTGAT ATCAGAAGCC 
ACTGTCTTTG CCCAGGCCAA CCAGCTGGAG AAATACAGAG TCATGTTTAC AGGTGGATCG 
TCGGTGAAGC AGGACAGCAA GCAGGTGCAG GTGGACCTCC AGGACCTGGG CTATGAGACC 
TGTGGCCGAA GCGAGAACGA GGCTGAGCGG GAGGAGAGCA CCAGCCCTGA GTGTGAGGAG 
CACGACAGCC TCAGGGAAAC AGTCTTGATG GAGGGGCTGT GCTCCAAGCA GGGACACCTG 
GACTCCACAA TGACCAGCCC CCCTGGGAAG AAGCCCCTGG AGAGCCAACC AGGGAAGCAG 
GAGGAGCTCC GGGCGTATGG AAAGTCAGAA GACATCTCTG TCCTACGTAA GGACATCAAA 
GATCTGAAGG CCCAGCTGCA GAACGCCAAC AAGGTCATTC AGAACCTGAA GAGCCGGGTT 
CGGTCCCTCT CAGTTACAAG CGATTATTCA TCTAGTCTGG AGAGACCCCG AAAGCTGAAG 
GCCGTTGGTG CCCTAGAAGG AACTTCACCG CACAGTGTCA CTGATGAGGA CGAGGGGTGG 
CTGTCTGATG GCACTGGGGC TTTCTACCCT CCAGGGCTTC AGGCTAAAAA GGATCTGGAG 
AGTCTGATCC AGAGGGTGTC CCAGCTAGAA GCCCAGCTCC CAAAAACTGG AACGGAAGGG 
AAGCTGGCCG AGGAGCTGAG ATCTGCTTCG TGGCCTGGGA AATATGATTC CCTGATTCAG 
GACCAGGCCC GAGAACTGTC CTACCTACGT CAGAAAATAC GGGAAGGGAG AGGTATCTGT 
TATCTTCTCA CCCAGCACTC GAAAGACACA GTAAAGTCTT TCGAGGACCT CCTAAGGAGC 
AATGACATTG ACTACTACCT GGGACAGAGC TTCCGGGAGC ACCTCGCCCA GGGCAGCCAG 
CTGACAGAAA GGCTCACCAG CAAACTCAGC ACCAAGGATC ATAAAAGTGA GAAAGATCAA 
GCTGGACTCG AGCCACTGGC TCTGAGGCTT AGCAGGGAGC TGCAGGAGAA GGAGAAAGTG 
ATCGAAGTCC TGCAGGCCAA GCTGGACGCC CGCTCCCTCA CACCGTGCAG CAGCCATGCC 
TTGTCAGACT CTCAGCGCTC TCCCAGCAGC TCCTCCTTCT TGTCTGACGA GCTGGAAGCC 
TGCTCTGACA TGGACATCGC CAGCGAGTAC ACACACTATG AAGAGAAGAA GGCTTCACCT 
GGTCACTCAG ATTCCATCCA TCATTCGAGT CATTCTGCTG TACTGTCTTC CAAACCATCA 
GCAACCAGTT CATCTCAGGA GGTTAAGGCC GAACCCAGCA GCAACCCCGT CAGCTTCCCA 
GCTCCCCAGA ATCCCCCCAA GGAGGCCAGC CAGGCCCATC CAGGCCTTCA TTTCCAATCC 
ATACCCACGC TGGTTAGCCT TCCCCAGGCA CCACTGCCCT CAGCTCCACC CAGCTTCCTG 
CCTTTCAGCT CTGCTGGCCC TCCCCTCCCT GGCTGCTGTG AGACACCCAT GGTCTCCTTG 
GCAGAGGCTC AGCAGGAGCT GCAGATGCTG CGGAAGCAGT TAGGAGAAAG TGTCACCAGT 
GTTCATCCTG CTTCCCCAGC CACACTGCCA ACCAACCACA CAGAAGCCAG ATCTTCCCAC 
TACCTCAACC CTCCCCAGCC CCTCTGTCCT CCAAGGGGCA CCACGGACCT GGGCAGAATC 
CTGGAGCCTG GGTACCTGGG TAGCAGTGGC CAGTGGGACG TGATGAGGCC TCAGAAAGGG 
AGCATATCCG GGGACCTATC CTCAGGCTCC TCCATGTACC AGCTTAACTC CAAGCCCACA 
GGGGCTGACC TGCTAGAGGA GCATCTTGGT GAAATCCGGA ACCTACGCCA GCGCCTGGAG 
GAGTCCATCT GCATTAATGA CCGCCTGCGG GAGCAGCTGG AAAACAGGCT CAGCTCCACT 
GCCCGCGAAA GTGGCTCAAC CTCTAACTTC TACAGTCCAG GCCTGGAGTC CACACCTCAG 
CTCTCCAATG AGAACAGAGT CCTTAGGGAA GAAAACCACA GGCTTCAGGC TCAGCTCAGT 
CATGTTTCCA GAGAGCACTC CCAGGAAACA GAATGCCTGA GGGAGGCTCT GCTGACCTCT 
CGATCCCGGC TTCAAGAGCT GGAAATGGAG CTGGAGCACC AAAAGGTAGA CCGGCAGCAG 
TTACTGGAAG ACCTGAAGGA GAAGCAGCAG GAGATCTTGC ATTTCCAGGA GGAACGACTG 
TCTCTCCAGG AGAAAGACTC CAGGCTGCAG CGTAAGCTGG CCCTCCTGCA GCAACAGTGC 
GAAGAGAAAC AGCAGCTCTT CCAGTCCCTC CAGTCAGAGC TACAGATCTA TGAGGCACTT 
TATGGTGACT CCAAGAAGAC GCTGAAAGCT TATACCTGGG ATGCCTGTCA CCAAGTCCCT 
TTGAGCAGTG ACTTGAGCCA CCTGGTGGCA GAGATCCGAG CTTTGCGAGG GCAGCTGGAG 
CAGAGCATTC AGGTGAACAA CTGTCTGCGA TTGCAGCTCG AACAGCAGTT GGATGGTGGT 
GCCAGCAAAG CTGGCCTCAG CTCCTCCTCC GTTAACCAGA AATTCCCCAC CAACACTGAC 
CCTGGAAACA AGCAGCCATT CTTCCAAGAT TCGGTTGCCT CCCCTCCAGT TCGGGATGTG 
GGCATGAATT CTCCAGTTCT CGTCTTCCCC AGCTCTTCTT CTGCGGCTCC TGGCCCAGAG 
ACCACAACCT TCAGTAGGAC GAACGAGCTG GGTTTGGATG CTTCTCCAGT AATGAAGAAC 
CCTCGCAAGC TGGAGGGCGA CGCTACTGAT GGCTCCTTTG CCAATAAGCA TGGCCGCCAC 
GTCATTGGCC ACATTGATGA CTATAGTGCC CTAAGAGAGC AGATTGGGGA GGGCAGACTG 
CTGGTCAAGA AGATAGCATC ACTGGTGAGA CCAACCTGCA GCTTCTCTGG TCTTGAAGCT 
CCAGGCATAG AGGTGATGGG CAGCGAAGGC ATCCAGGAGC TGAGGAGCAG CGCCACCGCC 
CTGCACCACC GGCTGGAGGA GTCGGCCTCC CTCCTCACCA TGTTCTGGCG AGCAGCCTTG 
CCAAGCTCCC CCGGCCCTGT GCTTGTGGAC AAAGTGGGAG AGTCTATGAA AAAGGAGCTT 
CTGGAACTGA GAACCAAACT ATCCAAACAG GAAAGTCTCC TTCAGAGTAC ATCTGAGCGT 
CTGAAGACCG CCAACCAGCA GAAGGAGAGC ATGGAGCAGT TCATCTTCAG CCAGTTGACC 
AGGACACACG ACGTTTTGAA GAAGGCAAGG ACTAATTTGG AGAAGACCTC TTACAAGACT 
GCTTCTGTAA AGCCTTATTC CTGTCCCAGC AAAGGTGAAA TCCCTAAGGG CCCTGCCATG 
CACTCCAGTC CTGTGACCCT TGCCTTCCAG GAGACATGCA AGAAGCGAAG CCACCAGAGG 
TCCTTAAAAC AGCAGGAAGG ATGGGCCTGT GCCCCTTCTT CACAGCTCCC TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_005204053.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204110.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X8, mRNA.

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CloneID	OBa17542
Clone ID Related Accession (Same CDS sequence)	XM_005204053.2 , XM_010826851.1
Accession Version	XM_005204053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005204053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGC CTTCATTTCC AATCCATACC CACGCTGGTT 
AGCCTTCCCC AGGCACCACT GCCCTCAGCT CCACCCAGCT TCCTGCCTTT CAGCTCTGCT 
GGCCCTCCCC TCCCTGGCTG CTGTGAGACA CCCATGGTCT CCTTGGCAGA GGCTCAGCAG 
GAGCTGCAGA TGCTGCGGAA GCAGTTAGGA GAAAGTGTCA CCAGTGTTCA TCCTGCTTCC 
CCAGCCACAC TGCCAACCAA CCACACAGAA GCCAGATCTT CCCACTACCT CAACCCTCCC 
CAGCCCCTCT GTCCTCCAAG GGGCACCACG GACCTGGGCA GAATCCTGGA GCCTGGGTAC 
CTGGGTAGCA GTGGCCAGTG GGACGTGATG AGGCCTCAGA AAGGGAGCAT ATCCGGGGAC 
CTATCCTCAG GCTCCTCCAT GTACCAGCTT AACTCCAAGC CCACAGGGGC TGACCTGCTA 
GAGGAGCATC TTGGTGAAAT CCGGAACCTA CGCCAGCGCC TGGAGGAGTC CATCTGCATT 
AATGACCGCC TGCGGGAGCA GCTGGAAAAC AGGCTCAGCT CCACTGCCCG CGAAAGTGGC 
TCAACCTCTA ACTTCTACAG TCCAGGCCTG GAGTCCACAC CTCAGCTCTC CAATGAGAAC 
AGAGTCCTTA GGGAAGAAAA CCACAGGCTT CAGGCTCAGC TCAGTCATGT TTCCAGAGAG 
CACTCCCAGG AAACAGAATG CCTGAGGGAG GCTCTGCTGA CCTCTCGATC CCGGCTTCAA 
GAGCTGGAAA TGGAGCTGGA GCACCAAAAG GTAGACCGGC AGCAGTTACT GGAAGACCTG 
AAGGAGAAGC AGCAGGAGAT CTTGCATTTC CAGGAGGAAC GACTGTCTCT CCAGGAGAAA 
GACTCCAGGC TGCAGCGTAA GCTGGCCCTC CTGCAGCAAC AGTGCGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CACTTTATGG TGACTCCAAG 
AAGACGCTGA AAGCTTATAC CTGGGATGCC TGTCACCAAG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAGAG CATTCAGGTG 
AACAACTGTC TGCGATTGCA GCTCGAACAG CAGTTGGATG GTGGTGCCAG CAAAGCTGGC 
CTCAGCTCCT CCTCCGTTAA CCAGAAATTC CCCACCAACA CTGACCCTGG AAACAAGCAG 
CCATTCTTCC AAGATTCGGT TGCCTCCCCT CCAGTTCGGG ATGTGGGCAT GAATTCTCCA 
GTTCTCGTCT TCCCCAGCTC TTCTTCTGCG GCTCCTGGCC CAGAGACCAC AACCTTCAGT 
AGGACGAACG AGCTGGGTTT GGATGCTTCT CCAGTAATGA AGAACCCTCG CAAGCTGGAG 
GGCGACGCTA CTGATGGCTC CTTTGCCAAT AAGCATGGCC GCCACGTCAT TGGCCACATT 
GATGACTATA GTGCCCTAAG AGAGCAGATT GGGGAGGGCA GACTGCTGGT CAAGAAGATA 
GCATCACTGG TGAGACCAAC CTGCAGCTTC TCTGGTCTTG AAGCTCCAGG CATAGAGCCA 
CAGGGATGCC CATGGCTACT GCCCCTTTCC CAGGTGATGG GCAGCGAAGG CATCCAGGAG 
CTGAGGAGCA GCGCCACCGC CCTGCACCAC CGGCTGGAGG AGTCGGCCTC CCTCCTCACC 
ATGTTCTGGC GAGCAGCCTT GCCAAGCTCC CCCGGCCCTG TGCTTGTGGA CAAAGTGGGA 
GAGTCTATGA AAAAGGAGCT TCTGGAACTG AGAACCAAAC TATCCAAACA GGAAAGTCTC 
CTTCAGAGTA CATCTGAGCG TCTGAAGACC GCCAACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCAGTTGAC CAGGACACAC GACGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGAAGACCT CTTACAAGAC TGCTTCTGTA AAGCCTTATT CCTGTCCCAG CAAAGGTGAA 
ATCCCTAAGG GCCCTGCCAT GCACTCCAGT CCTGTGACCC TTGCCTTCCA GGAGACATGC 
AAGAAGCGAA GCCACCAGAG GTCCTTAAAA CAGCAGGAAG GATGGGCCTG TGCCCCTTCT 
TCACAGCTCC CTGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204110.1
CDS	16..7656
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_005204053.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204053.2

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGC CTTCATTTCC AATCCATACC CACGCTGGTT 
AGCCTTCCCC AGGCACCACT GCCCTCAGCT CCACCCAGCT TCCTGCCTTT CAGCTCTGCT 
GGCCCTCCCC TCCCTGGCTG CTGTGAGACA CCCATGGTCT CCTTGGCAGA GGCTCAGCAG 
GAGCTGCAGA TGCTGCGGAA GCAGTTAGGA GAAAGTGTCA CCAGTGTTCA TCCTGCTTCC 
CCAGCCACAC TGCCAACCAA CCACACAGAA GCCAGATCTT CCCACTACCT CAACCCTCCC 
CAGCCCCTCT GTCCTCCAAG GGGCACCACG GACCTGGGCA GAATCCTGGA GCCTGGGTAC 
CTGGGTAGCA GTGGCCAGTG GGACGTGATG AGGCCTCAGA AAGGGAGCAT ATCCGGGGAC 
CTATCCTCAG GCTCCTCCAT GTACCAGCTT AACTCCAAGC CCACAGGGGC TGACCTGCTA 
GAGGAGCATC TTGGTGAAAT CCGGAACCTA CGCCAGCGCC TGGAGGAGTC CATCTGCATT 
AATGACCGCC TGCGGGAGCA GCTGGAAAAC AGGCTCAGCT CCACTGCCCG CGAAAGTGGC 
TCAACCTCTA ACTTCTACAG TCCAGGCCTG GAGTCCACAC CTCAGCTCTC CAATGAGAAC 
AGAGTCCTTA GGGAAGAAAA CCACAGGCTT CAGGCTCAGC TCAGTCATGT TTCCAGAGAG 
CACTCCCAGG AAACAGAATG CCTGAGGGAG GCTCTGCTGA CCTCTCGATC CCGGCTTCAA 
GAGCTGGAAA TGGAGCTGGA GCACCAAAAG GTAGACCGGC AGCAGTTACT GGAAGACCTG 
AAGGAGAAGC AGCAGGAGAT CTTGCATTTC CAGGAGGAAC GACTGTCTCT CCAGGAGAAA 
GACTCCAGGC TGCAGCGTAA GCTGGCCCTC CTGCAGCAAC AGTGCGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CACTTTATGG TGACTCCAAG 
AAGACGCTGA AAGCTTATAC CTGGGATGCC TGTCACCAAG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAGAG CATTCAGGTG 
AACAACTGTC TGCGATTGCA GCTCGAACAG CAGTTGGATG GTGGTGCCAG CAAAGCTGGC 
CTCAGCTCCT CCTCCGTTAA CCAGAAATTC CCCACCAACA CTGACCCTGG AAACAAGCAG 
CCATTCTTCC AAGATTCGGT TGCCTCCCCT CCAGTTCGGG ATGTGGGCAT GAATTCTCCA 
GTTCTCGTCT TCCCCAGCTC TTCTTCTGCG GCTCCTGGCC CAGAGACCAC AACCTTCAGT 
AGGACGAACG AGCTGGGTTT GGATGCTTCT CCAGTAATGA AGAACCCTCG CAAGCTGGAG 
GGCGACGCTA CTGATGGCTC CTTTGCCAAT AAGCATGGCC GCCACGTCAT TGGCCACATT 
GATGACTATA GTGCCCTAAG AGAGCAGATT GGGGAGGGCA GACTGCTGGT CAAGAAGATA 
GCATCACTGG TGAGACCAAC CTGCAGCTTC TCTGGTCTTG AAGCTCCAGG CATAGAGCCA 
CAGGGATGCC CATGGCTACT GCCCCTTTCC CAGGTGATGG GCAGCGAAGG CATCCAGGAG 
CTGAGGAGCA GCGCCACCGC CCTGCACCAC CGGCTGGAGG AGTCGGCCTC CCTCCTCACC 
ATGTTCTGGC GAGCAGCCTT GCCAAGCTCC CCCGGCCCTG TGCTTGTGGA CAAAGTGGGA 
GAGTCTATGA AAAAGGAGCT TCTGGAACTG AGAACCAAAC TATCCAAACA GGAAAGTCTC 
CTTCAGAGTA CATCTGAGCG TCTGAAGACC GCCAACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCAGTTGAC CAGGACACAC GACGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGAAGACCT CTTACAAGAC TGCTTCTGTA AAGCCTTATT CCTGTCCCAG CAAAGGTGAA 
ATCCCTAAGG GCCCTGCCAT GCACTCCAGT CCTGTGACCC TTGCCTTCCA GGAGACATGC 
AAGAAGCGAA GCCACCAGAG GTCCTTAAAA CAGCAGGAAG GATGGGCCTG TGCCCCTTCT 
TCACAGCTCC CTGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826851.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825153.1 Bos taurus myomegalin (LOC508547), transcript variant X5, mRNA.

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CloneID	OBa17542
Clone ID Related Accession (Same CDS sequence)	XM_005204053.2 , XM_010826851.1
Accession Version	XM_010826851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGC CTTCATTTCC AATCCATACC CACGCTGGTT 
AGCCTTCCCC AGGCACCACT GCCCTCAGCT CCACCCAGCT TCCTGCCTTT CAGCTCTGCT 
GGCCCTCCCC TCCCTGGCTG CTGTGAGACA CCCATGGTCT CCTTGGCAGA GGCTCAGCAG 
GAGCTGCAGA TGCTGCGGAA GCAGTTAGGA GAAAGTGTCA CCAGTGTTCA TCCTGCTTCC 
CCAGCCACAC TGCCAACCAA CCACACAGAA GCCAGATCTT CCCACTACCT CAACCCTCCC 
CAGCCCCTCT GTCCTCCAAG GGGCACCACG GACCTGGGCA GAATCCTGGA GCCTGGGTAC 
CTGGGTAGCA GTGGCCAGTG GGACGTGATG AGGCCTCAGA AAGGGAGCAT ATCCGGGGAC 
CTATCCTCAG GCTCCTCCAT GTACCAGCTT AACTCCAAGC CCACAGGGGC TGACCTGCTA 
GAGGAGCATC TTGGTGAAAT CCGGAACCTA CGCCAGCGCC TGGAGGAGTC CATCTGCATT 
AATGACCGCC TGCGGGAGCA GCTGGAAAAC AGGCTCAGCT CCACTGCCCG CGAAAGTGGC 
TCAACCTCTA ACTTCTACAG TCCAGGCCTG GAGTCCACAC CTCAGCTCTC CAATGAGAAC 
AGAGTCCTTA GGGAAGAAAA CCACAGGCTT CAGGCTCAGC TCAGTCATGT TTCCAGAGAG 
CACTCCCAGG AAACAGAATG CCTGAGGGAG GCTCTGCTGA CCTCTCGATC CCGGCTTCAA 
GAGCTGGAAA TGGAGCTGGA GCACCAAAAG GTAGACCGGC AGCAGTTACT GGAAGACCTG 
AAGGAGAAGC AGCAGGAGAT CTTGCATTTC CAGGAGGAAC GACTGTCTCT CCAGGAGAAA 
GACTCCAGGC TGCAGCGTAA GCTGGCCCTC CTGCAGCAAC AGTGCGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CACTTTATGG TGACTCCAAG 
AAGACGCTGA AAGCTTATAC CTGGGATGCC TGTCACCAAG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAGAG CATTCAGGTG 
AACAACTGTC TGCGATTGCA GCTCGAACAG CAGTTGGATG GTGGTGCCAG CAAAGCTGGC 
CTCAGCTCCT CCTCCGTTAA CCAGAAATTC CCCACCAACA CTGACCCTGG AAACAAGCAG 
CCATTCTTCC AAGATTCGGT TGCCTCCCCT CCAGTTCGGG ATGTGGGCAT GAATTCTCCA 
GTTCTCGTCT TCCCCAGCTC TTCTTCTGCG GCTCCTGGCC CAGAGACCAC AACCTTCAGT 
AGGACGAACG AGCTGGGTTT GGATGCTTCT CCAGTAATGA AGAACCCTCG CAAGCTGGAG 
GGCGACGCTA CTGATGGCTC CTTTGCCAAT AAGCATGGCC GCCACGTCAT TGGCCACATT 
GATGACTATA GTGCCCTAAG AGAGCAGATT GGGGAGGGCA GACTGCTGGT CAAGAAGATA 
GCATCACTGG TGAGACCAAC CTGCAGCTTC TCTGGTCTTG AAGCTCCAGG CATAGAGCCA 
CAGGGATGCC CATGGCTACT GCCCCTTTCC CAGGTGATGG GCAGCGAAGG CATCCAGGAG 
CTGAGGAGCA GCGCCACCGC CCTGCACCAC CGGCTGGAGG AGTCGGCCTC CCTCCTCACC 
ATGTTCTGGC GAGCAGCCTT GCCAAGCTCC CCCGGCCCTG TGCTTGTGGA CAAAGTGGGA 
GAGTCTATGA AAAAGGAGCT TCTGGAACTG AGAACCAAAC TATCCAAACA GGAAAGTCTC 
CTTCAGAGTA CATCTGAGCG TCTGAAGACC GCCAACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCAGTTGAC CAGGACACAC GACGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGAAGACCT CTTACAAGAC TGCTTCTGTA AAGCCTTATT CCTGTCCCAG CAAAGGTGAA 
ATCCCTAAGG GCCCTGCCAT GCACTCCAGT CCTGTGACCC TTGCCTTCCA GGAGACATGC 
AAGAAGCGAA GCCACCAGAG GTCCTTAAAA CAGCAGGAAG GATGGGCCTG TGCCCCTTCT 
TCACAGCTCC CTGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825153.1
CDS	16..7656
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_010826851.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826851.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGC CTTCATTTCC AATCCATACC CACGCTGGTT 
AGCCTTCCCC AGGCACCACT GCCCTCAGCT CCACCCAGCT TCCTGCCTTT CAGCTCTGCT 
GGCCCTCCCC TCCCTGGCTG CTGTGAGACA CCCATGGTCT CCTTGGCAGA GGCTCAGCAG 
GAGCTGCAGA TGCTGCGGAA GCAGTTAGGA GAAAGTGTCA CCAGTGTTCA TCCTGCTTCC 
CCAGCCACAC TGCCAACCAA CCACACAGAA GCCAGATCTT CCCACTACCT CAACCCTCCC 
CAGCCCCTCT GTCCTCCAAG GGGCACCACG GACCTGGGCA GAATCCTGGA GCCTGGGTAC 
CTGGGTAGCA GTGGCCAGTG GGACGTGATG AGGCCTCAGA AAGGGAGCAT ATCCGGGGAC 
CTATCCTCAG GCTCCTCCAT GTACCAGCTT AACTCCAAGC CCACAGGGGC TGACCTGCTA 
GAGGAGCATC TTGGTGAAAT CCGGAACCTA CGCCAGCGCC TGGAGGAGTC CATCTGCATT 
AATGACCGCC TGCGGGAGCA GCTGGAAAAC AGGCTCAGCT CCACTGCCCG CGAAAGTGGC 
TCAACCTCTA ACTTCTACAG TCCAGGCCTG GAGTCCACAC CTCAGCTCTC CAATGAGAAC 
AGAGTCCTTA GGGAAGAAAA CCACAGGCTT CAGGCTCAGC TCAGTCATGT TTCCAGAGAG 
CACTCCCAGG AAACAGAATG CCTGAGGGAG GCTCTGCTGA CCTCTCGATC CCGGCTTCAA 
GAGCTGGAAA TGGAGCTGGA GCACCAAAAG GTAGACCGGC AGCAGTTACT GGAAGACCTG 
AAGGAGAAGC AGCAGGAGAT CTTGCATTTC CAGGAGGAAC GACTGTCTCT CCAGGAGAAA 
GACTCCAGGC TGCAGCGTAA GCTGGCCCTC CTGCAGCAAC AGTGCGAAGA GAAACAGCAG 
CTCTTCCAGT CCCTCCAGTC AGAGCTACAG ATCTATGAGG CACTTTATGG TGACTCCAAG 
AAGACGCTGA AAGCTTATAC CTGGGATGCC TGTCACCAAG TCCCTTTGAG CAGTGACTTG 
AGCCACCTGG TGGCAGAGAT CCGAGCTTTG CGAGGGCAGC TGGAGCAGAG CATTCAGGTG 
AACAACTGTC TGCGATTGCA GCTCGAACAG CAGTTGGATG GTGGTGCCAG CAAAGCTGGC 
CTCAGCTCCT CCTCCGTTAA CCAGAAATTC CCCACCAACA CTGACCCTGG AAACAAGCAG 
CCATTCTTCC AAGATTCGGT TGCCTCCCCT CCAGTTCGGG ATGTGGGCAT GAATTCTCCA 
GTTCTCGTCT TCCCCAGCTC TTCTTCTGCG GCTCCTGGCC CAGAGACCAC AACCTTCAGT 
AGGACGAACG AGCTGGGTTT GGATGCTTCT CCAGTAATGA AGAACCCTCG CAAGCTGGAG 
GGCGACGCTA CTGATGGCTC CTTTGCCAAT AAGCATGGCC GCCACGTCAT TGGCCACATT 
GATGACTATA GTGCCCTAAG AGAGCAGATT GGGGAGGGCA GACTGCTGGT CAAGAAGATA 
GCATCACTGG TGAGACCAAC CTGCAGCTTC TCTGGTCTTG AAGCTCCAGG CATAGAGCCA 
CAGGGATGCC CATGGCTACT GCCCCTTTCC CAGGTGATGG GCAGCGAAGG CATCCAGGAG 
CTGAGGAGCA GCGCCACCGC CCTGCACCAC CGGCTGGAGG AGTCGGCCTC CCTCCTCACC 
ATGTTCTGGC GAGCAGCCTT GCCAAGCTCC CCCGGCCCTG TGCTTGTGGA CAAAGTGGGA 
GAGTCTATGA AAAAGGAGCT TCTGGAACTG AGAACCAAAC TATCCAAACA GGAAAGTCTC 
CTTCAGAGTA CATCTGAGCG TCTGAAGACC GCCAACCAGC AGAAGGAGAG CATGGAGCAG 
TTCATCTTCA GCCAGTTGAC CAGGACACAC GACGTTTTGA AGAAGGCAAG GACTAATTTG 
GAGAAGACCT CTTACAAGAC TGCTTCTGTA AAGCCTTATT CCTGTCCCAG CAAAGGTGAA 
ATCCCTAAGG GCCCTGCCAT GCACTCCAGT CCTGTGACCC TTGCCTTCCA GGAGACATGC 
AAGAAGCGAA GCCACCAGAG GTCCTTAAAA CAGCAGGAAG GATGGGCCTG TGCCCCTTCT 
TCACAGCTCC CTGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010803130.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801432.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X32, mRNA.

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CloneID	OBa47203
Clone ID Related Accession (Same CDS sequence)	XM_010803130.1 , XM_010826857.1
Accession Version	XM_010803130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGT GTCACCAGTG TTCATCCTGC TTCCCCAGCC 
ACACTGCCAA CCAACCACAC AGAAGCCAGA TCTTCCCACT ACCTCAACCC TCCCCAGCCC 
CTCTGTCCTC CAAGGGGCAC CACGGACCTG GGCAGAATCC TGGAGCCTGG GTACCTGGGT 
AGCAGTGGCC AGTGGGACGT GATGAGGCCT CAGAAAGGGA GCATATCCGG GGACCTATCC 
TCAGGCTCCT CCATGTACCA GCTTAACTCC AAGCCCACAG GGGCTGACCT GCTAGAGGAG 
CATCTTGGTG AAATCCGGAA CCTACGCCAG CGCCTGGAGG AGTCCATCTG CATTAATGAC 
CGCCTGCGGG AGCAGCTGGA AAACAGGCTC AGCTCCACTG CCCGCGAAAG TGGCTCAACC 
TCTAACTTCT ACAGTCCAGG CCTGGAGTCC ACACCTCAGC TCTCCAATGA GAACAGAGTC 
CTTAGGGAAG AAAACCACAG GCTTCAGGCT CAGCTCAGTC ATGTTTCCAG AGAGCACTCC 
CAGGAAACAG AATGCCTGAG GGAGGCTCTG CTGACCTCTC GATCCCGGCT TCAAGAGCTG 
GAAATGGAGC TGGAGCACCA AAAGGTAGAC CGGCAGCAGT TACTGGAAGA CCTGAAGGAG 
AAGCAGCAGG AGATCTTGCA TTTCCAGGAG GAACGACTGT CTCTCCAGGA GAAAGACTCC 
AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG CAACAGTGCG AAGAGAAACA GCAGCTCTTC 
CAGTCCCTCC AGTCAGAGCT ACAGATCTAT GAGGCACTTT ATGGTGACTC CAAGAAGACG 
CTGAAAGCTT ATACCTGGGA TGCCTGTCAC CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC 
CTGGTGGCAG AGATCCGAGC TTTGCGAGGG CAGCTGGAGC AGAGCATTCA GGTGAACAAC 
TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG GATGGTGGTG CCAGCAAAGC TGGCCTCAGC 
TCCTCCTCCG TTAACCAGAA ATTCCCCACC AACACTGACC CTGGAAACAA GCAGCCATTC 
TTCCAAGATT CGGTTGCCTC CCCTCCAGTT CGGGATGTGG GCATGAATTC TCCAGTTCTC 
GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT GGCCCAGAGA CCACAACCTT CAGTAGGACG 
AACGAGCTGG GTTTGGATGC TTCTCCAGTA ATGAAGAACC CTCGCAAGCT GGAGGGCGAC 
GCTACTGATG GCTCCTTTGC CAATAAGCAT GGCCGCCACG TCATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGAGAGCA GATTGGGGAG GGCAGACTGC TGGTCAAGAA GATAGCATCA 
CTGGTGAGAC CAACCTGCAG CTTCTCTGGT CTTGAAGCTC CAGGCATAGA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GGTGAAATCC CTAAGGGCCC TGCCATGCAC TCCAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801432.1
CDS	16..7278
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_010803130.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803130.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGT GTCACCAGTG TTCATCCTGC TTCCCCAGCC 
ACACTGCCAA CCAACCACAC AGAAGCCAGA TCTTCCCACT ACCTCAACCC TCCCCAGCCC 
CTCTGTCCTC CAAGGGGCAC CACGGACCTG GGCAGAATCC TGGAGCCTGG GTACCTGGGT 
AGCAGTGGCC AGTGGGACGT GATGAGGCCT CAGAAAGGGA GCATATCCGG GGACCTATCC 
TCAGGCTCCT CCATGTACCA GCTTAACTCC AAGCCCACAG GGGCTGACCT GCTAGAGGAG 
CATCTTGGTG AAATCCGGAA CCTACGCCAG CGCCTGGAGG AGTCCATCTG CATTAATGAC 
CGCCTGCGGG AGCAGCTGGA AAACAGGCTC AGCTCCACTG CCCGCGAAAG TGGCTCAACC 
TCTAACTTCT ACAGTCCAGG CCTGGAGTCC ACACCTCAGC TCTCCAATGA GAACAGAGTC 
CTTAGGGAAG AAAACCACAG GCTTCAGGCT CAGCTCAGTC ATGTTTCCAG AGAGCACTCC 
CAGGAAACAG AATGCCTGAG GGAGGCTCTG CTGACCTCTC GATCCCGGCT TCAAGAGCTG 
GAAATGGAGC TGGAGCACCA AAAGGTAGAC CGGCAGCAGT TACTGGAAGA CCTGAAGGAG 
AAGCAGCAGG AGATCTTGCA TTTCCAGGAG GAACGACTGT CTCTCCAGGA GAAAGACTCC 
AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG CAACAGTGCG AAGAGAAACA GCAGCTCTTC 
CAGTCCCTCC AGTCAGAGCT ACAGATCTAT GAGGCACTTT ATGGTGACTC CAAGAAGACG 
CTGAAAGCTT ATACCTGGGA TGCCTGTCAC CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC 
CTGGTGGCAG AGATCCGAGC TTTGCGAGGG CAGCTGGAGC AGAGCATTCA GGTGAACAAC 
TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG GATGGTGGTG CCAGCAAAGC TGGCCTCAGC 
TCCTCCTCCG TTAACCAGAA ATTCCCCACC AACACTGACC CTGGAAACAA GCAGCCATTC 
TTCCAAGATT CGGTTGCCTC CCCTCCAGTT CGGGATGTGG GCATGAATTC TCCAGTTCTC 
GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT GGCCCAGAGA CCACAACCTT CAGTAGGACG 
AACGAGCTGG GTTTGGATGC TTCTCCAGTA ATGAAGAACC CTCGCAAGCT GGAGGGCGAC 
GCTACTGATG GCTCCTTTGC CAATAAGCAT GGCCGCCACG TCATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGAGAGCA GATTGGGGAG GGCAGACTGC TGGTCAAGAA GATAGCATCA 
CTGGTGAGAC CAACCTGCAG CTTCTCTGGT CTTGAAGCTC CAGGCATAGA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GGTGAAATCC CTAAGGGCCC TGCCATGCAC TCCAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_010826857.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825159.1 Bos taurus myomegalin (LOC508547), transcript variant X22, mRNA.

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CloneID	OBa47203
Clone ID Related Accession (Same CDS sequence)	XM_010803130.1 , XM_010826857.1
Accession Version	XM_010826857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGT GTCACCAGTG TTCATCCTGC TTCCCCAGCC 
ACACTGCCAA CCAACCACAC AGAAGCCAGA TCTTCCCACT ACCTCAACCC TCCCCAGCCC 
CTCTGTCCTC CAAGGGGCAC CACGGACCTG GGCAGAATCC TGGAGCCTGG GTACCTGGGT 
AGCAGTGGCC AGTGGGACGT GATGAGGCCT CAGAAAGGGA GCATATCCGG GGACCTATCC 
TCAGGCTCCT CCATGTACCA GCTTAACTCC AAGCCCACAG GGGCTGACCT GCTAGAGGAG 
CATCTTGGTG AAATCCGGAA CCTACGCCAG CGCCTGGAGG AGTCCATCTG CATTAATGAC 
CGCCTGCGGG AGCAGCTGGA AAACAGGCTC AGCTCCACTG CCCGCGAAAG TGGCTCAACC 
TCTAACTTCT ACAGTCCAGG CCTGGAGTCC ACACCTCAGC TCTCCAATGA GAACAGAGTC 
CTTAGGGAAG AAAACCACAG GCTTCAGGCT CAGCTCAGTC ATGTTTCCAG AGAGCACTCC 
CAGGAAACAG AATGCCTGAG GGAGGCTCTG CTGACCTCTC GATCCCGGCT TCAAGAGCTG 
GAAATGGAGC TGGAGCACCA AAAGGTAGAC CGGCAGCAGT TACTGGAAGA CCTGAAGGAG 
AAGCAGCAGG AGATCTTGCA TTTCCAGGAG GAACGACTGT CTCTCCAGGA GAAAGACTCC 
AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG CAACAGTGCG AAGAGAAACA GCAGCTCTTC 
CAGTCCCTCC AGTCAGAGCT ACAGATCTAT GAGGCACTTT ATGGTGACTC CAAGAAGACG 
CTGAAAGCTT ATACCTGGGA TGCCTGTCAC CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC 
CTGGTGGCAG AGATCCGAGC TTTGCGAGGG CAGCTGGAGC AGAGCATTCA GGTGAACAAC 
TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG GATGGTGGTG CCAGCAAAGC TGGCCTCAGC 
TCCTCCTCCG TTAACCAGAA ATTCCCCACC AACACTGACC CTGGAAACAA GCAGCCATTC 
TTCCAAGATT CGGTTGCCTC CCCTCCAGTT CGGGATGTGG GCATGAATTC TCCAGTTCTC 
GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT GGCCCAGAGA CCACAACCTT CAGTAGGACG 
AACGAGCTGG GTTTGGATGC TTCTCCAGTA ATGAAGAACC CTCGCAAGCT GGAGGGCGAC 
GCTACTGATG GCTCCTTTGC CAATAAGCAT GGCCGCCACG TCATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGAGAGCA GATTGGGGAG GGCAGACTGC TGGTCAAGAA GATAGCATCA 
CTGGTGAGAC CAACCTGCAG CTTCTCTGGT CTTGAAGCTC CAGGCATAGA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GGTGAAATCC CTAAGGGCCC TGCCATGCAC TCCAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825159.1
CDS	16..7278
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFTGGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_010826857.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826857.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTACAGGTG GATCGTCGGT GAAGCAGGAC 
AGCAAGCAGG TGCAGGTGGA CCTCCAGGAC CTGGGCTATG AGACCTGTGG CCGAAGCGAG 
AACGAGGCTG AGCGGGAGGA GAGCACCAGC CCTGAGTGTG AGGAGCACGA CAGCCTCAGG 
GAAACAGTCT TGATGGAGGG GCTGTGCTCC AAGCAGGGAC ACCTGGACTC CACAATGACC 
AGCCCCCCTG GGAAGAAGCC CCTGGAGAGC CAACCAGGGA AGCAGGAGGA GCTCCGGGCG 
TATGGAAAGT CAGAAGACAT CTCTGTCCTA CGTAAGGACA TCAAAGATCT GAAGGCCCAG 
CTGCAGAACG CCAACAAGGT CATTCAGAAC CTGAAGAGCC GGGTTCGGTC CCTCTCAGTT 
ACAAGCGATT ATTCATCTAG TCTGGAGAGA CCCCGAAAGC TGAAGGCCGT TGGTGCCCTA 
GAAGGAACTT CACCGCACAG TGTCACTGAT GAGGACGAGG GGTGGCTGTC TGATGGCACT 
GGGGCTTTCT ACCCTCCAGG GCTTCAGGCT AAAAAGGATC TGGAGAGTCT GATCCAGAGG 
GTGTCCCAGC TAGAAGCCCA GCTCCCAAAA ACTGGAACGG AAGGGAAGCT GGCCGAGGAG 
CTGAGATCTG CTTCGTGGCC TGGGAAATAT GATTCCCTGA TTCAGGACCA GGCCCGAGAA 
CTGTCCTACC TACGTCAGAA AATACGGGAA GGGAGAGGTA TCTGTTATCT TCTCACCCAG 
CACTCGAAAG ACACAGTAAA GTCTTTCGAG GACCTCCTAA GGAGCAATGA CATTGACTAC 
TACCTGGGAC AGAGCTTCCG GGAGCACCTC GCCCAGGGCA GCCAGCTGAC AGAAAGGCTC 
ACCAGCAAAC TCAGCACCAA GGATCATAAA AGTGAGAAAG ATCAAGCTGG ACTCGAGCCA 
CTGGCTCTGA GGCTTAGCAG GGAGCTGCAG GAGAAGGAGA AAGTGATCGA AGTCCTGCAG 
GCCAAGCTGG ACGCCCGCTC CCTCACACCG TGCAGCAGCC ATGCCTTGTC AGACTCTCAG 
CGCTCTCCCA GCAGCTCCTC CTTCTTGTCT GACGAGCTGG AAGCCTGCTC TGACATGGAC 
ATCGCCAGCG AGTACACACA CTATGAAGAG AAGAAGGCTT CACCTGGTCA CTCAGATTCC 
ATCCATCATT CGAGTCATTC TGCTGTACTG TCTTCCAAAC CATCAGCAAC CAGTTCATCT 
CAGGAGGTTA AGGCCGAACC CAGCAGCAAC CCCGTCAGCT TCCCAGCTCC CCAGAATCCC 
CCCAAGGAGG CCAGCCAGGC CCATCCAGGT GTCACCAGTG TTCATCCTGC TTCCCCAGCC 
ACACTGCCAA CCAACCACAC AGAAGCCAGA TCTTCCCACT ACCTCAACCC TCCCCAGCCC 
CTCTGTCCTC CAAGGGGCAC CACGGACCTG GGCAGAATCC TGGAGCCTGG GTACCTGGGT 
AGCAGTGGCC AGTGGGACGT GATGAGGCCT CAGAAAGGGA GCATATCCGG GGACCTATCC 
TCAGGCTCCT CCATGTACCA GCTTAACTCC AAGCCCACAG GGGCTGACCT GCTAGAGGAG 
CATCTTGGTG AAATCCGGAA CCTACGCCAG CGCCTGGAGG AGTCCATCTG CATTAATGAC 
CGCCTGCGGG AGCAGCTGGA AAACAGGCTC AGCTCCACTG CCCGCGAAAG TGGCTCAACC 
TCTAACTTCT ACAGTCCAGG CCTGGAGTCC ACACCTCAGC TCTCCAATGA GAACAGAGTC 
CTTAGGGAAG AAAACCACAG GCTTCAGGCT CAGCTCAGTC ATGTTTCCAG AGAGCACTCC 
CAGGAAACAG AATGCCTGAG GGAGGCTCTG CTGACCTCTC GATCCCGGCT TCAAGAGCTG 
GAAATGGAGC TGGAGCACCA AAAGGTAGAC CGGCAGCAGT TACTGGAAGA CCTGAAGGAG 
AAGCAGCAGG AGATCTTGCA TTTCCAGGAG GAACGACTGT CTCTCCAGGA GAAAGACTCC 
AGGCTGCAGC GTAAGCTGGC CCTCCTGCAG CAACAGTGCG AAGAGAAACA GCAGCTCTTC 
CAGTCCCTCC AGTCAGAGCT ACAGATCTAT GAGGCACTTT ATGGTGACTC CAAGAAGACG 
CTGAAAGCTT ATACCTGGGA TGCCTGTCAC CAAGTCCCTT TGAGCAGTGA CTTGAGCCAC 
CTGGTGGCAG AGATCCGAGC TTTGCGAGGG CAGCTGGAGC AGAGCATTCA GGTGAACAAC 
TGTCTGCGAT TGCAGCTCGA ACAGCAGTTG GATGGTGGTG CCAGCAAAGC TGGCCTCAGC 
TCCTCCTCCG TTAACCAGAA ATTCCCCACC AACACTGACC CTGGAAACAA GCAGCCATTC 
TTCCAAGATT CGGTTGCCTC CCCTCCAGTT CGGGATGTGG GCATGAATTC TCCAGTTCTC 
GTCTTCCCCA GCTCTTCTTC TGCGGCTCCT GGCCCAGAGA CCACAACCTT CAGTAGGACG 
AACGAGCTGG GTTTGGATGC TTCTCCAGTA ATGAAGAACC CTCGCAAGCT GGAGGGCGAC 
GCTACTGATG GCTCCTTTGC CAATAAGCAT GGCCGCCACG TCATTGGCCA CATTGATGAC 
TATAGTGCCC TAAGAGAGCA GATTGGGGAG GGCAGACTGC TGGTCAAGAA GATAGCATCA 
CTGGTGAGAC CAACCTGCAG CTTCTCTGGT CTTGAAGCTC CAGGCATAGA GGTGATGGGC 
AGCGAAGGCA TCCAGGAGCT GAGGAGCAGC GCCACCGCCC TGCACCACCG GCTGGAGGAG 
TCGGCCTCCC TCCTCACCAT GTTCTGGCGA GCAGCCTTGC CAAGCTCCCC CGGCCCTGTG 
CTTGTGGACA AAGTGGGAGA GTCTATGAAA AAGGAGCTTC TGGAACTGAG AACCAAACTA 
TCCAAACAGG AAAGTCTCCT TCAGAGTACA TCTGAGCGTC TGAAGACCGC CAACCAGCAG 
AAGGAGAGCA TGGAGCAGTT CATCTTCAGC CAGTTGACCA GGACACACGA CGTTTTGAAG 
AAGGCAAGGA CTAATTTGGA GGTGAAATCC CTAAGGGCCC TGCCATGCAC TCCAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015462608.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318094.1 Bos taurus myomegalin (LOC508547), transcript variant X6, mRNA.

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CloneID	OBa89534
Clone ID Related Accession (Same CDS sequence)	XM_015462608.1 , XM_015463419.1
Accession Version	XM_015462608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGCCACAG 
GGATGCCCAT GGCTACTGCC CCTTTCCCAG GTGATGGGCA GCGAAGGCAT CCAGGAGCTG 
AGGAGCAGCG CCACCGCCCT GCACCACCGG CTGGAGGAGT CGGCCTCCCT CCTCACCATG 
TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC GGCCCTGTGC TTGTGGACAA AGTGGGAGAG 
TCTATGAAAA AGGAGCTTCT GGAACTGAGA ACCAAACTAT CCAAACAGGA AAGTCTCCTT 
CAGAGTACAT CTGAGCGTCT GAAGACCGCC AACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC AGTTGACCAG GACACACGAC GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
AAGACCTCTT ACAAGACTGC TTCTGTAAAG CCTTATTCCT GTCCCAGCAA AGGTGAAATC 
CCTAAGGGCC CTGCCATGCA CTCCAGTCCT GTGACCCTTG CCTTCCAGGA GACATGCAAG 
AAGCGAAGCC ACCAGAGGTC CTTAAAACAG CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA 
CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318094.1
CDS	16..7653
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015462608.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462608.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGCCACAG 
GGATGCCCAT GGCTACTGCC CCTTTCCCAG GTGATGGGCA GCGAAGGCAT CCAGGAGCTG 
AGGAGCAGCG CCACCGCCCT GCACCACCGG CTGGAGGAGT CGGCCTCCCT CCTCACCATG 
TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC GGCCCTGTGC TTGTGGACAA AGTGGGAGAG 
TCTATGAAAA AGGAGCTTCT GGAACTGAGA ACCAAACTAT CCAAACAGGA AAGTCTCCTT 
CAGAGTACAT CTGAGCGTCT GAAGACCGCC AACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC AGTTGACCAG GACACACGAC GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
AAGACCTCTT ACAAGACTGC TTCTGTAAAG CCTTATTCCT GTCCCAGCAA AGGTGAAATC 
CCTAAGGGCC CTGCCATGCA CTCCAGTCCT GTGACCCTTG CCTTCCAGGA GACATGCAAG 
AAGCGAAGCC ACCAGAGGTC CTTAAAACAG CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA 
CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015463419.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318905.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X9, mRNA.

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CloneID	OBa89534
Clone ID Related Accession (Same CDS sequence)	XM_015462608.1 , XM_015463419.1
Accession Version	XM_015463419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGCCACAG 
GGATGCCCAT GGCTACTGCC CCTTTCCCAG GTGATGGGCA GCGAAGGCAT CCAGGAGCTG 
AGGAGCAGCG CCACCGCCCT GCACCACCGG CTGGAGGAGT CGGCCTCCCT CCTCACCATG 
TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC GGCCCTGTGC TTGTGGACAA AGTGGGAGAG 
TCTATGAAAA AGGAGCTTCT GGAACTGAGA ACCAAACTAT CCAAACAGGA AAGTCTCCTT 
CAGAGTACAT CTGAGCGTCT GAAGACCGCC AACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC AGTTGACCAG GACACACGAC GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
AAGACCTCTT ACAAGACTGC TTCTGTAAAG CCTTATTCCT GTCCCAGCAA AGGTGAAATC 
CCTAAGGGCC CTGCCATGCA CTCCAGTCCT GTGACCCTTG CCTTCCAGGA GACATGCAAG 
AAGCGAAGCC ACCAGAGGTC CTTAAAACAG CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA 
CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318905.1
CDS	16..7653
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEPQGCPWLLPLSQVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEKTSYKTASVKPYSCPSKGEIPKGPAMHSSPVTLAFQETCKKRSHQRSLKQQEGWACAPSSQLPVC

Target ORF information:

RefSeq Version	XM_015463419.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463419.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGCCACAG 
GGATGCCCAT GGCTACTGCC CCTTTCCCAG GTGATGGGCA GCGAAGGCAT CCAGGAGCTG 
AGGAGCAGCG CCACCGCCCT GCACCACCGG CTGGAGGAGT CGGCCTCCCT CCTCACCATG 
TTCTGGCGAG CAGCCTTGCC AAGCTCCCCC GGCCCTGTGC TTGTGGACAA AGTGGGAGAG 
TCTATGAAAA AGGAGCTTCT GGAACTGAGA ACCAAACTAT CCAAACAGGA AAGTCTCCTT 
CAGAGTACAT CTGAGCGTCT GAAGACCGCC AACCAGCAGA AGGAGAGCAT GGAGCAGTTC 
ATCTTCAGCC AGTTGACCAG GACACACGAC GTTTTGAAGA AGGCAAGGAC TAATTTGGAG 
AAGACCTCTT ACAAGACTGC TTCTGTAAAG CCTTATTCCT GTCCCAGCAA AGGTGAAATC 
CCTAAGGGCC CTGCCATGCA CTCCAGTCCT GTGACCCTTG CCTTCCAGGA GACATGCAAG 
AAGCGAAGCC ACCAGAGGTC CTTAAAACAG CAGGAAGGAT GGGCCTGTGC CCCTTCTTCA 
CAGCTCCCTG TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015463430.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318916.1 Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X20, mRNA.

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CloneID	OBa89535
Clone ID Related Accession (Same CDS sequence)	XM_015463430.1 , XM_015462609.1
Accession Version	XM_015463430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myomegalin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318916.1
CDS	16..7461
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_015463430.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463430.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE4DIP ( XM_015462609.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318095.1 Bos taurus myomegalin (LOC508547), transcript variant X12, mRNA.

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CloneID	OBa89535
Clone ID Related Accession (Same CDS sequence)	XM_015463430.1 , XM_015462609.1
Accession Version	XM_015462609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myomegalin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318095.1
CDS	16..7461
Translation	MKRIDSGSCCRRRCDCGCCCRAVRRAHHAPYWSRDAARIPPSSRQSPGRERRHPEPAGSWAAAAEEEEAAAAAAPWMRDYFAEDDGEMVPRTSHAAAFLSDTKDRGPPLQSQTWRSGDKIPLGHSLRAFERPLQVQTQALRDFEKHLNDLKKENFSLKLRIYFLEERMQQKYEASRDDIYKRNIELKVEVESLKRELQDKRQHPDKTCADAENINSHNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLELSEKLKGVTKEREDVPGDRVKSDQYTEALAQRDKKIEELSQSLAAQEKLVEQLSQEKQQLLHLLEEPADMEVQPMTEDLLKQQKLNSNETGTTQQSVSDSHLAELQEKIQQTETTNKMLQEKLNEMSYELKSAQESSQKQDDTIQNLKETLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLKQLHNSEGTSPAQQQVALLDLQSALFCSQLEIQKLQRAVRQKERQLADARRCVRFVEAAAQEREQQKEASWKHNQELQKALQQLQGELQSKSQQLHTLEAEKYNEIRTQEQHIQHLHYSLSHKEQLLQEFRELLQYRNNSDKTLEANEMLLEKLRQRIQDRDVALERAIDEKFSSLEEKEKELRQLHLAMRERDHDLERLRGILSANEATMQSMESLLRSKGLEVEQLSATCQNLQWLKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLTATLLCKLGPGQTEIVEELCQRLQQKERMLQDLLSDRNKQAVEYEMEIQGLLQSMSTREQESHAAAEKMVQALMERNSELQALRQYLGGKDFMMSQAPLSNRPAEVTSISPHLGEQTDQGSVHIPSRDDSTSLTAKGDASVPRSSLGEVDTVAGLEKELSNAKEELELMAKKERESRMELSALQSMVAVQEQELQVQAADMESLTRTIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREKVASVESQGQETSGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGAQVIRNRLEEVLGRSLERLSRLETLAAIGGTAAGDDTEDASTEFTDSIEEEAAHNSHQQLIKVALEKSRAAVETQNVSPAPPTLTGGDGNRGLQEEMLHLRAEIHRHLEEKRKAEGELKELKAQIEEAGFSSVAHIRNTMLSLCLENAELKEQMGEAMSDGWEIEEDKEKGEAMVETVVAKGVLNENSLQAEFRKVQGKLKNARNIISLLKEQLVLSSKEGTSKLNPELLVHLAKEINRMNTELVYSPGKHQCLEEEGVTTRPCPRPQSLDLGAALTVDAHQLDNQPQTHDPGPQSEFSFSGSTKHLRSQLAQCKQRYQDLQEKLLISEATVFAQANQLEKYRVMFSGSSVKQDSKQVQVDLQDLGYETCGRSENEAEREESTSPECEEHDSLRETVLMEGLCSKQGHLDSTMTSPPGKKPLESQPGKQEELRAYGKSEDISVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPRKLKAVGALEGTSPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGTEGKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHSKDTVKSFEDLLRSNDIDYYLGQSFREHLAQGSQLTERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPCSSHALSDSQRSPSSSSFLSDELEACSDMDIASEYTHYEEKKASPGHSDSIHHSSHSAVLSSKPSATSSSQEVKAEPSSNPVSFPAPQNPPKEASQAHPGLHFQSIPTLVSLPQAPLPSAPPSFLPFSSAGPPLPGCCETPMVSLAEAQQELQMLRKQLGESVTSVHPASPATLPTNHTEARSSHYLNPPQPLCPPRGTTDLGRILEPGYLGSSGQWDVMRPQKGSISGDLSSGSSMYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLENRLSSTARESGSTSNFYSPGLESTPQLSNENRVLREENHRLQAQLSHVSREHSQETECLREALLTSRSRLQELEMELEHQKVDRQQLLEDLKEKQQEILHFQEERLSLQEKDSRLQRKLALLQQQCEEKQQLFQSLQSELQIYEALYGDSKKTLKAYTWDACHQVPLSSDLSHLVAEIRALRGQLEQSIQVNNCLRLQLEQQLDGGASKAGLSSSSVNQKFPTNTDPGNKQPFFQDSVASPPVRDVGMNSPVLVFPSSSSAAPGPETTTFSRTNELGLDASPVMKNPRKLEGDATDGSFANKHGRHVIGHIDDYSALREQIGEGRLLVKKIASLVRPTCSFSGLEAPGIEVMGSEGIQELRSSATALHHRLEESASLLTMFWRAALPSSPGPVLVDKVGESMKKELLELRTKLSKQESLLQSTSERLKTANQQKESMEQFIFSQLTRTHDVLKKARTNLEVKSLRALPCTPVL

Target ORF information:

RefSeq Version	XM_015462609.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myomegalin (LOC508547), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462609.1

ORF Insert Sequence:

ATGAAGAGGA TTGACAGCGG GTCCTGCTGC CGCCGCCGCT GCGACTGCGG CTGCTGCTGC 
CGCGCGGTCC GCCGGGCGCA CCACGCGCCC TACTGGTCCA GGGACGCGGC GCGAATCCCG 
CCGTCCTCGC GGCAGTCTCC CGGCCGGGAA CGACGGCATC CCGAGCCGGC CGGGAGCTGG 
GCAGCAGCGG CCGAAGAGGA AGAGGCAGCC GCGGCAGCCG CACCCTGGAT GAGAGATTAT 
TTTGCAGAAG ATGATGGGGA GATGGTACCC AGAACAAGTC ATGCAGCAGC TTTTCTTAGT 
GACACCAAAG ATCGAGGCCC TCCTCTGCAG TCACAGACTT GGAGAAGTGG TGACAAGATC 
CCCTTGGGGC ATTCCTTGAG AGCATTTGAA AGACCTCTTC AGGTGCAAAC CCAGGCCCTT 
AGAGACTTTG AGAAGCACCT CAATGACCTG AAGAAGGAGA ACTTCAGCCT CAAGCTGCGC 
ATCTACTTCC TGGAGGAGCG CATGCAGCAG AAGTATGAGG CCAGCCGGGA TGACATCTAC 
AAGCGGAACA TCGAGCTGAA GGTTGAAGTA GAGAGCCTGA AACGAGAACT CCAGGACAAG 
AGACAGCATC CGGATAAAAC ATGCGCTGAT GCAGAGAATA TCAACAGCCA TAATGAAGCT 
GAGCTCCGGC GCCAGTTTGA GGAGAGACAG CAGGAGACGG AGCATGTTTA CGAGCTCCTG 
GAGAATAAGA TCCAGCTTCT GCAAGAGGAG TCCAGACTAG CAAAAAATGA AGCCGCACGG 
ATGGCGGCTC TGGTGGAAGC AGAGAAAGAG TGTAACCTGG AGCTCTCAGA GAAACTGAAG 
GGAGTCACCA AGGAGAGGGA AGATGTACCA GGAGACCGGG TCAAATCTGA CCAGTACACT 
GAGGCCCTGG CCCAGAGGGA CAAAAAAATT GAAGAACTGA GTCAAAGCCT GGCTGCACAG 
GAGAAACTTG TAGAGCAGCT GTCTCAAGAG AAACAACAAC TGCTACATCT GTTGGAGGAG 
CCAGCTGACA TGGAAGTGCA GCCCATGACT GAAGACTTAC TCAAACAACA GAAGTTGAAT 
TCAAATGAGA CCGGTACAAC TCAGCAGTCT GTATCTGATT CCCATCTGGC AGAACTCCAG 
GAAAAAATCC AGCAAACAGA GACCACCAAC AAGATGCTTC AAGAAAAACT GAATGAAATG 
AGCTATGAAC TGAAGTCTGC TCAGGAGTCA TCTCAGAAGC AAGATGATAC AATTCAAAAC 
CTCAAGGAAA CTCTGAAAAG CAGGGAAAGT GAGACCGAGG AGTTGTACCA GGTGATTGAA 
GGTCAAAACG ACACAATGGC TAAGCTTCGA GAAATGCTGC ACCAGAGTCA GCTTAAACAG 
CTTCATAACT CAGAGGGTAC TTCCCCAGCT CAGCAACAGG TGGCTCTGCT TGATCTTCAA 
AGTGCTTTAT TCTGCAGCCA GCTTGAAATA CAGAAGCTCC AGAGGGCAGT ACGCCAAAAA 
GAAAGGCAGC TGGCTGATGC TAGACGATGT GTGCGGTTTG TAGAGGCTGC AGCACAGGAG 
AGAGAACAGC AGAAAGAGGC TTCTTGGAAA CATAACCAAG AATTGCAAAA AGCCCTACAG 
CAGCTACAAG GAGAACTTCA GAGTAAGAGC CAACAGCTTC ATACCCTGGA GGCTGAAAAA 
TATAATGAGA TTCGCACCCA AGAGCAGCAC ATTCAGCACC TCCACTACAG TCTGAGTCAC 
AAAGAGCAGC TGCTTCAGGA ATTTCGGGAG CTCCTGCAGT ACCGAAATAA CTCAGACAAA 
ACCCTTGAAG CAAATGAAAT GTTGCTTGAG AAACTTAGGC AAAGAATACA AGATAGAGAT 
GTTGCCCTAG AGCGGGCTAT AGATGAAAAG TTCTCCAGCC TAGAAGAAAA GGAAAAAGAA 
CTGCGTCAGC TTCACCTTGC CATGAGAGAG CGAGATCACG ACTTAGAGAG GCTGCGCGGT 
ATCCTCTCCG CCAATGAAGC TACCATGCAG AGTATGGAGA GTCTCCTGAG GTCCAAAGGC 
CTGGAAGTGG AACAGTTGTC TGCCACCTGT CAGAACCTCC AGTGGCTGAA AGAAGAAATG 
GAAACCAAAT TTAGCCGTTG GCAGAAGGAG CAAGAAAGCA TCATTCAGCA GCTACAGACA 
TCTCTGCATG ACAGGAACAA AGAAGTGGAG GATCTTACCG CAACATTGCT CTGCAAACTT 
GGACCAGGGC AGACTGAGAT AGTTGAGGAG CTGTGCCAGC GTCTACAGCA GAAGGAAAGG 
ATGCTGCAGG ACCTTCTGAG TGATCGGAAC AAGCAAGCAG TGGAATATGA AATGGAGATT 
CAGGGCCTGC TTCAGTCCAT GAGCACCAGA GAGCAGGAGA GCCACGCTGC TGCAGAAAAG 
ATGGTACAAG CTCTAATGGA AAGAAATTCA GAATTACAGG CCCTGCGCCA ATATTTAGGA 
GGGAAAGATT TCATGATGTC CCAAGCACCC CTCTCTAACC GACCAGCTGA AGTTACCTCC 
ATCAGCCCCC ATCTTGGAGA GCAAACTGAT CAAGGTTCAG TGCATATACC CTCCAGAGAT 
GATAGCACTT CATTGACTGC CAAAGGGGAT GCTAGCGTAC CCAGGTCCTC GTTAGGGGAG 
GTGGATACAG TTGCGGGGCT GGAAAAAGAA CTGAGTAATG CCAAAGAGGA ACTTGAGCTC 
ATGGCTAAAA AAGAGAGAGA AAGTCGGATG GAACTGTCTG CACTCCAGTC CATGGTGGCT 
GTGCAGGAGC AAGAGCTGCA GGTTCAGGCT GCTGACATGG AGTCCCTGAC CAGGACCATA 
CAGATTAAGG AAGACCTCAT AAAGGACCTG CAGATGCAGC TGGTTGATCC TGAAGACATC 
CCAGCTATGG AACGCCTGAC CCAGGAAGTC TTGCTTCTTC GGGAAAAAGT GGCTTCAGTA 
GAATCACAAG GACAGGAAAC GTCTGGAAAC CGAAGACAAC AATTGCTGCT GATGCTCGAA 
GGACTGGTAG ATGAGCGGAG TCGGCTCAAT GAGGCCTTAC AGGCAGAGAG ACAGCTCTAT 
AGCAGTCTGG TGAAGTTCCA TGCCCATCCA GAGAGCTCTG AGAGAGACCG AACTCTGCAG 
GTGGAACTAG AAGGGGCCCA GGTGATACGC AATCGGCTAG AAGAAGTTCT CGGAAGAAGC 
CTGGAGCGCT TAAGCAGGCT GGAGACCCTG GCCGCCATTG GAGGTACAGC TGCAGGGGAC 
GACACGGAGG ATGCGAGCAC GGAGTTTACC GACAGTATCG AGGAGGAGGC TGCACACAAC 
AGCCACCAGC AACTCATCAA GGTGGCGTTG GAGAAAAGCC GGGCAGCTGT GGAGACCCAG 
AATGTGTCTC CTGCTCCTCC CACCCTGACA GGAGGGGACG GTAACAGGGG TCTTCAGGAG 
GAAATGCTCC ACCTGAGGGC TGAAATTCAC CGGCACTTAG AGGAGAAGAG GAAAGCTGAG 
GGGGAACTGA AGGAGCTAAA GGCTCAAATT GAGGAAGCAG GATTCTCCTC TGTGGCCCAC 
ATCAGGAACA CCATGCTGAG CCTTTGCCTT GAGAATGCAG AGCTGAAAGA GCAGATGGGA 
GAAGCAATGT CTGATGGATG GGAGATTGAG GAAGACAAGG AGAAGGGCGA GGCGATGGTG 
GAGACTGTGG TTGCCAAAGG GGTTCTGAAT GAGAATAGCC TTCAGGCTGA ATTCAGAAAG 
GTCCAGGGAA AACTGAAGAA TGCCCGCAAT ATCATCAGCC TCCTCAAAGA ACAGCTGGTG 
TTGAGCAGCA AGGAAGGGAC TAGTAAGCTT AATCCTGAGC TCCTTGTGCA CCTGGCCAAG 
GAGATCAACA GAATGAACAC AGAGCTGGTT TACTCTCCTG GAAAGCACCA ATGCCTAGAA 
GAGGAGGGTG TGACCACAAG GCCGTGCCCC AGACCCCAGA GCCTTGACCT TGGGGCTGCC 
CTCACAGTGG ATGCTCACCA ACTGGATAAC CAGCCCCAGA CCCATGACCC TGGGCCGCAG 
TCAGAATTTA GCTTCTCAGG ATCCACGAAG CACCTGCGCT CCCAGTTGGC ACAATGCAAA 
CAACGCTATC AGGATCTCCA GGAGAAGCTG CTGATATCAG AAGCCACTGT CTTTGCCCAG 
GCCAACCAGC TGGAGAAATA CAGAGTCATG TTTAGTGGAT CGTCGGTGAA GCAGGACAGC 
AAGCAGGTGC AGGTGGACCT CCAGGACCTG GGCTATGAGA CCTGTGGCCG AAGCGAGAAC 
GAGGCTGAGC GGGAGGAGAG CACCAGCCCT GAGTGTGAGG AGCACGACAG CCTCAGGGAA 
ACAGTCTTGA TGGAGGGGCT GTGCTCCAAG CAGGGACACC TGGACTCCAC AATGACCAGC 
CCCCCTGGGA AGAAGCCCCT GGAGAGCCAA CCAGGGAAGC AGGAGGAGCT CCGGGCGTAT 
GGAAAGTCAG AAGACATCTC TGTCCTACGT AAGGACATCA AAGATCTGAA GGCCCAGCTG 
CAGAACGCCA ACAAGGTCAT TCAGAACCTG AAGAGCCGGG TTCGGTCCCT CTCAGTTACA 
AGCGATTATT CATCTAGTCT GGAGAGACCC CGAAAGCTGA AGGCCGTTGG TGCCCTAGAA 
GGAACTTCAC CGCACAGTGT CACTGATGAG GACGAGGGGT GGCTGTCTGA TGGCACTGGG 
GCTTTCTACC CTCCAGGGCT TCAGGCTAAA AAGGATCTGG AGAGTCTGAT CCAGAGGGTG 
TCCCAGCTAG AAGCCCAGCT CCCAAAAACT GGAACGGAAG GGAAGCTGGC CGAGGAGCTG 
AGATCTGCTT CGTGGCCTGG GAAATATGAT TCCCTGATTC AGGACCAGGC CCGAGAACTG 
TCCTACCTAC GTCAGAAAAT ACGGGAAGGG AGAGGTATCT GTTATCTTCT CACCCAGCAC 
TCGAAAGACA CAGTAAAGTC TTTCGAGGAC CTCCTAAGGA GCAATGACAT TGACTACTAC 
CTGGGACAGA GCTTCCGGGA GCACCTCGCC CAGGGCAGCC AGCTGACAGA AAGGCTCACC 
AGCAAACTCA GCACCAAGGA TCATAAAAGT GAGAAAGATC AAGCTGGACT CGAGCCACTG 
GCTCTGAGGC TTAGCAGGGA GCTGCAGGAG AAGGAGAAAG TGATCGAAGT CCTGCAGGCC 
AAGCTGGACG CCCGCTCCCT CACACCGTGC AGCAGCCATG CCTTGTCAGA CTCTCAGCGC 
TCTCCCAGCA GCTCCTCCTT CTTGTCTGAC GAGCTGGAAG CCTGCTCTGA CATGGACATC 
GCCAGCGAGT ACACACACTA TGAAGAGAAG AAGGCTTCAC CTGGTCACTC AGATTCCATC 
CATCATTCGA GTCATTCTGC TGTACTGTCT TCCAAACCAT CAGCAACCAG TTCATCTCAG 
GAGGTTAAGG CCGAACCCAG CAGCAACCCC GTCAGCTTCC CAGCTCCCCA GAATCCCCCC 
AAGGAGGCCA GCCAGGCCCA TCCAGGCCTT CATTTCCAAT CCATACCCAC GCTGGTTAGC 
CTTCCCCAGG CACCACTGCC CTCAGCTCCA CCCAGCTTCC TGCCTTTCAG CTCTGCTGGC 
CCTCCCCTCC CTGGCTGCTG TGAGACACCC ATGGTCTCCT TGGCAGAGGC TCAGCAGGAG 
CTGCAGATGC TGCGGAAGCA GTTAGGAGAA AGTGTCACCA GTGTTCATCC TGCTTCCCCA 
GCCACACTGC CAACCAACCA CACAGAAGCC AGATCTTCCC ACTACCTCAA CCCTCCCCAG 
CCCCTCTGTC CTCCAAGGGG CACCACGGAC CTGGGCAGAA TCCTGGAGCC TGGGTACCTG 
GGTAGCAGTG GCCAGTGGGA CGTGATGAGG CCTCAGAAAG GGAGCATATC CGGGGACCTA 
TCCTCAGGCT CCTCCATGTA CCAGCTTAAC TCCAAGCCCA CAGGGGCTGA CCTGCTAGAG 
GAGCATCTTG GTGAAATCCG GAACCTACGC CAGCGCCTGG AGGAGTCCAT CTGCATTAAT 
GACCGCCTGC GGGAGCAGCT GGAAAACAGG CTCAGCTCCA CTGCCCGCGA AAGTGGCTCA 
ACCTCTAACT TCTACAGTCC AGGCCTGGAG TCCACACCTC AGCTCTCCAA TGAGAACAGA 
GTCCTTAGGG AAGAAAACCA CAGGCTTCAG GCTCAGCTCA GTCATGTTTC CAGAGAGCAC 
TCCCAGGAAA CAGAATGCCT GAGGGAGGCT CTGCTGACCT CTCGATCCCG GCTTCAAGAG 
CTGGAAATGG AGCTGGAGCA CCAAAAGGTA GACCGGCAGC AGTTACTGGA AGACCTGAAG 
GAGAAGCAGC AGGAGATCTT GCATTTCCAG GAGGAACGAC TGTCTCTCCA GGAGAAAGAC 
TCCAGGCTGC AGCGTAAGCT GGCCCTCCTG CAGCAACAGT GCGAAGAGAA ACAGCAGCTC 
TTCCAGTCCC TCCAGTCAGA GCTACAGATC TATGAGGCAC TTTATGGTGA CTCCAAGAAG 
ACGCTGAAAG CTTATACCTG GGATGCCTGT CACCAAGTCC CTTTGAGCAG TGACTTGAGC 
CACCTGGTGG CAGAGATCCG AGCTTTGCGA GGGCAGCTGG AGCAGAGCAT TCAGGTGAAC 
AACTGTCTGC GATTGCAGCT CGAACAGCAG TTGGATGGTG GTGCCAGCAA AGCTGGCCTC 
AGCTCCTCCT CCGTTAACCA GAAATTCCCC ACCAACACTG ACCCTGGAAA CAAGCAGCCA 
TTCTTCCAAG ATTCGGTTGC CTCCCCTCCA GTTCGGGATG TGGGCATGAA TTCTCCAGTT 
CTCGTCTTCC CCAGCTCTTC TTCTGCGGCT CCTGGCCCAG AGACCACAAC CTTCAGTAGG 
ACGAACGAGC TGGGTTTGGA TGCTTCTCCA GTAATGAAGA ACCCTCGCAA GCTGGAGGGC 
GACGCTACTG ATGGCTCCTT TGCCAATAAG CATGGCCGCC ACGTCATTGG CCACATTGAT 
GACTATAGTG CCCTAAGAGA GCAGATTGGG GAGGGCAGAC TGCTGGTCAA GAAGATAGCA 
TCACTGGTGA GACCAACCTG CAGCTTCTCT GGTCTTGAAG CTCCAGGCAT AGAGGTGATG 
GGCAGCGAAG GCATCCAGGA GCTGAGGAGC AGCGCCACCG CCCTGCACCA CCGGCTGGAG 
GAGTCGGCCT CCCTCCTCAC CATGTTCTGG CGAGCAGCCT TGCCAAGCTC CCCCGGCCCT 
GTGCTTGTGG ACAAAGTGGG AGAGTCTATG AAAAAGGAGC TTCTGGAACT GAGAACCAAA 
CTATCCAAAC AGGAAAGTCT CCTTCAGAGT ACATCTGAGC GTCTGAAGAC CGCCAACCAG 
CAGAAGGAGA GCATGGAGCA GTTCATCTTC AGCCAGTTGA CCAGGACACA CGACGTTTTG 
AAGAAGGCAA GGACTAATTT GGAGGTGAAA TCCCTAAGGG CCCTGCCATG CACTCCAGTC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PDE4DIP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: