UBE3C cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE3C
Entrez Gene ID	508513
Full Name	ubiquitin protein ligase E3C
General protein information	Preferred Names ubiquitin protein ligase E3C Names ubiquitin-protein ligase E3C
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015470754.1	XP_015326240.1	ubiquitin-protein ligase E3C isoform X2
NM_001192188.1	NP_001179117.1	ubiquitin-protein ligase E3C
XM_024990564.1	XP_024846332.1	ubiquitin-protein ligase E3C isoform X2
NM_001192188.3	NP_001179117.2	ubiquitin-protein ligase E3C
XM_005205800.1	XP_005205857.1	ubiquitin-protein ligase E3C isoform X1
XM_005205800.2	XP_005205857.1	ubiquitin-protein ligase E3C isoform X1
XM_015470754.2	XP_015326240.1	ubiquitin-protein ligase E3C isoform X3
NM_001192188.2	NP_001179117.2	ubiquitin-protein ligase E3C

Pathways from BioSystems

Homologies

	UBE3C	XP_002803585.1
Canis lupus familiaris (dog)	UBE3C	XP_539937.2
Pan troglodytes (chimpanzee)	UBE3C	XP_528010.3
Drosophila melanogaster (fruit fly)	CG3356	NP_611896.1
Gallus gallus (chicken)	UBE3C	NP_001026138.1
Bos taurus (cattle)	UBE3C	NP_001179117.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011474	XP_317832.4
Homo sapiens (human)	UBE3C	NP_055486.2
Mus musculus (house mouse)	Ube3c	NP_598668.1
Rattus norvegicus (Norway rat)	Ube3c	XP_006235923.1
Danio rerio (zebrafish)	ube3c	NP_956767.1
Xenopus tropicalis (tropical clawed frog)	ube3c	NP_001072518.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UBE3C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa216389	NM_001192188.2 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa239230	NM_001192188.3 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa233591	XM_005205800.2 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa233592	XM_024990564.1 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa233593	XM_015470754.2 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa90651	XM_015470754.1 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa38696	NM_001192188.1 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa18831	XM_005205800.1 Latest version!	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE3C ( NM_001192188.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179117.2 Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.

Price & Availability↑

CloneID	OBa216389
Clone ID Related Accession (Same CDS sequence)	NM_001192188.2
Accession Version	NM_001192188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02012154.1. On Jun 30, 2017 this sequence version replaced NM_001192188.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CTGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGGAG CTGTACCCCG CGTTCTGCAT CCACAACGGG 
GGCTCCGACC TGGAGCGGCT GCCCACAGCC AGCACCTGCA TGAACCTGCT GAAGCTCCCC 
GAGTTCCAGG ACGAGGCGCT GCTGCGGAGC AAGCTCCTCT ACGCCATCGA GTGTGCCGCG 
GGCTTCGAGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179117.2
CDS	1..3258
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDVGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFQDEALLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	NM_001192188.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192188.2

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CTGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGGAG CTGTACCCCG CGTTCTGCAT CCACAACGGG 
GGCTCCGACC TGGAGCGGCT GCCCACAGCC AGCACCTGCA TGAACCTGCT GAAGCTCCCC 
GAGTTCCAGG ACGAGGCGCT GCTGCGGAGC AAGCTCCTCT ACGCCATCGA GTGTGCCGCG 
GGCTTCGAGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( NM_001192188.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179117.2 Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.

Price & Availability↑

CloneID	OBa239230
Clone ID Related Accession (Same CDS sequence)	NM_001192188.3
Accession Version	NM_001192188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000004.1. On Dec 20, 2018 this sequence version replaced NM_001192188.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGVD01093260.1, GGUZ01082605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGGAG CTGTACCCCG CGTTCTGCAT CCACAACGGG 
GGCTCCGACC TGGAGCGGCT GCCCACAGCC AGCACCTGCA TGAACCTGCT GAAGCTCCCC 
GAGTTCCAGG ACGAGGCGCT GCTGCGGAGC AAGCTCCTCT ACGCCATCGA GTGTGCCGCG 
GGCTTCGAGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179117.2
CDS	1..3258
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDVGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFQDEALLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	NM_001192188.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192188.3

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGGAG CTGTACCCCG CGTTCTGCAT CCACAACGGG 
GGCTCCGACC TGGAGCGGCT GCCCACAGCC AGCACCTGCA TGAACCTGCT GAAGCTCCCC 
GAGTTCCAGG ACGAGGCGCT GCTGCGGAGC AAGCTCCTCT ACGCCATCGA GTGTGCCGCG 
GGCTTCGAGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_005205800.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205857.1 Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233591
Clone ID Related Accession (Same CDS sequence)	XM_005205800.2
Accession Version	XM_005205800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGGT CTGGAGTCTC CGCCGCTGTC CGTGTCTGAG 
GAGAGGCAGC TCGCCATCCT GACCCAGCTG CCCTTTGTGG TTCCGTTTGA GGAGCGGGTA 
AAGATCTTTC AGAGATTGAT TTATGCAGAT AAGCAAGAAG TTCAAGGAGA TGGTCCATTT 
CTGGATGGAA TTAATGTCAC AATAAGAAGG AATTATATTT ATGAAGATGC TTATGACAAA 
CTTTCCCCTG AAAATGAGCC TGACTTGAAA AAGCGGATCC GTGTGCACTT ACTGAATGCG 
CACGGCCTGG ACGAAGCCGG CATTGACGGT GGTGGTATTT TCAGAGAGTT TCTAAACGAA 
CTACTGAAGT CAGGGTTTAA CCCCAACCAG GGGTTCTTTA AGACCACTAA CGAAGGGCTT 
CTGTACCCCA ACCCAGCCGC TCAGATGCTT GTGGGCGATG CCTTTGCCAG ACATTACTAC 
TTCCTGGGCA GGATGCTCGG AAAGGCCCTC TATGAAAACA TGCTGGTGGA GCTGCCCTTC 
GCCGGCTTCT TCCTGTCCAA GCTGCTGGGG ACCAGCGCGG ACGTGGACAT CCACCACCTG 
GCGTCCTTAG ACCCCGAGGT GTACAGGAAC CTGCTCTTCC TCAAGAGCTA TGAGGGCGAC 
GTGGAGGAGC TGGGGCTGAA CTTCACGGTG GTGAACAACG ACCTCGGAGA GGCCCAGGTA 
GTTGAACTGA AATCTGGTGG GAAGGACATC CCGGTGACCA GTGCCAATCG CATTGCGTAC 
ATCCACCTGG TGGCCGACTA CCGGCTGAAC CGGCAGATCC GCCCGCACTG CCTGGCGTTC 
CGGCAGGGCC TGGCCAACGT GGTGAGCCTG GAGTGGCTGC GGATGTTCGA CCAGCAGGAG 
ATCCAGGTAT TGATTTCTGG TGCACAAGTT CCCATAAGTC TGGAGGACCT AAAATCCTTT 
ACAAACTATT CAGGAGGCTA CTCTGCCGAG CACCCCGTGA TCAAGGTCTT CTGGAGAGTC 
GTGGAGGGCT TCACGGACGA GGAGAAGCGC AAACTGCTCA AGTTTGTGAC CAGCTGCTCC 
CGGCCCCCCC TCTTGGGGTT TAAGGAGCTG TACCCCGCGT TCTGCATCCA CAACGGGGGC 
TCCGACCTGG AGCGGCTGCC CACAGCCAGC ACCTGCATGA ACCTGCTGAA GCTCCCCGAG 
TTCCAGGACG AGGCGCTGCT GCGGAGCAAG CTCCTCTACG CCATCGAGTG TGCCGCGGGC 
TTCGAGCTGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205857.1
CDS	316..3570
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFQDEALLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_005205800.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205800.2

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGGT CTGGAGTCTC CGCCGCTGTC CGTGTCTGAG 
GAGAGGCAGC TCGCCATCCT GACCCAGCTG CCCTTTGTGG TTCCGTTTGA GGAGCGGGTA 
AAGATCTTTC AGAGATTGAT TTATGCAGAT AAGCAAGAAG TTCAAGGAGA TGGTCCATTT 
CTGGATGGAA TTAATGTCAC AATAAGAAGG AATTATATTT ATGAAGATGC TTATGACAAA 
CTTTCCCCTG AAAATGAGCC TGACTTGAAA AAGCGGATCC GTGTGCACTT ACTGAATGCG 
CACGGCCTGG ACGAAGCCGG CATTGACGGT GGTGGTATTT TCAGAGAGTT TCTAAACGAA 
CTACTGAAGT CAGGGTTTAA CCCCAACCAG GGGTTCTTTA AGACCACTAA CGAAGGGCTT 
CTGTACCCCA ACCCAGCCGC TCAGATGCTT GTGGGCGATG CCTTTGCCAG ACATTACTAC 
TTCCTGGGCA GGATGCTCGG AAAGGCCCTC TATGAAAACA TGCTGGTGGA GCTGCCCTTC 
GCCGGCTTCT TCCTGTCCAA GCTGCTGGGG ACCAGCGCGG ACGTGGACAT CCACCACCTG 
GCGTCCTTAG ACCCCGAGGT GTACAGGAAC CTGCTCTTCC TCAAGAGCTA TGAGGGCGAC 
GTGGAGGAGC TGGGGCTGAA CTTCACGGTG GTGAACAACG ACCTCGGAGA GGCCCAGGTA 
GTTGAACTGA AATCTGGTGG GAAGGACATC CCGGTGACCA GTGCCAATCG CATTGCGTAC 
ATCCACCTGG TGGCCGACTA CCGGCTGAAC CGGCAGATCC GCCCGCACTG CCTGGCGTTC 
CGGCAGGGCC TGGCCAACGT GGTGAGCCTG GAGTGGCTGC GGATGTTCGA CCAGCAGGAG 
ATCCAGGTAT TGATTTCTGG TGCACAAGTT CCCATAAGTC TGGAGGACCT AAAATCCTTT 
ACAAACTATT CAGGAGGCTA CTCTGCCGAG CACCCCGTGA TCAAGGTCTT CTGGAGAGTC 
GTGGAGGGCT TCACGGACGA GGAGAAGCGC AAACTGCTCA AGTTTGTGAC CAGCTGCTCC 
CGGCCCCCCC TCTTGGGGTT TAAGGAGCTG TACCCCGCGT TCTGCATCCA CAACGGGGGC 
TCCGACCTGG AGCGGCTGCC CACAGCCAGC ACCTGCATGA ACCTGCTGAA GCTCCCCGAG 
TTCCAGGACG AGGCGCTGCT GCGGAGCAAG CTCCTCTACG CCATCGAGTG TGCCGCGGGC 
TTCGAGCTGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_024990564.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846332.1 Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa233592
Clone ID Related Accession (Same CDS sequence)	XM_024990564.1
Accession Version	XM_024990564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGACT CCGATCTGGG GAAAGCGCTG TGAGGCATGT 
GCTCCGGTTG GCTCAGGGTG CCTGCTGGCG GGGCGGCGGA CTCAGACTCG AAAGCAGAGG 
ATGCATCCTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846332.1
CDS	317..3511
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDVGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKTPIWGKRCEACAPVGSGCLLAGRRTQTRKQRMHPG

Target ORF information:

RefSeq Version	XM_024990564.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990564.1

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGACT CCGATCTGGG GAAAGCGCTG TGAGGCATGT 
GCTCCGGTTG GCTCAGGGTG CCTGCTGGCG GGGCGGCGGA CTCAGACTCG AAAGCAGAGG 
ATGCATCCTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_015470754.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326240.1 Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.

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CloneID	OBa233593
Clone ID Related Accession (Same CDS sequence)	XM_015470754.2
Accession Version	XM_015470754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326240.1
CDS	316..3408
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDVGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKS

Target ORF information:

RefSeq Version	XM_015470754.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470754.2

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GCTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_015470754.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326240.1 Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa90651
Clone ID Related Accession (Same CDS sequence)	XM_015470754.1
Accession Version	XM_015470754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CTGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326240.1
CDS	256..3348
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDVGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKS

Target ORF information:

RefSeq Version	XM_015470754.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470754.1

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CTGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( NM_001192188.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179117.1 Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.

Price & Availability↑

CloneID	OBa38696
Clone ID Related Accession (Same CDS sequence)	NM_001192188.1
Accession Version	NM_001192188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	ubiquitin-protein ligase E3C
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03097865.1, AAFC03117431.1 and AAFC03096037.1. On Jul 17, 2010 this sequence version replaced XM_585301.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACTCGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGGAG CTGTACCCCG CGTTCTGCAT CCACAACGGG 
GGCTCCGACC TGGAGCGGCT GCCCACAGCC AGCACCTGCA TGAACCTGCT GAAGCTCCCC 
GAGTTCCAGG ACGAGGCGCT GCTGCGGAGC AAGCTCCTCT ACGCCATCGA GTGTGCCGCG 
GGCTTCGAGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179117.1
CDS	1..3258
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDSKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDVGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFQDEALLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	NM_001192188.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192188.1

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CCGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACTCGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGTA GGTCTGGAGT CTCCGCCGCT GTCCGTGTCT 
GAGGAGAGGC AGCTCGCCAT CCTGACCCAG CTGCCCTTTG TGGTTCCGTT TGAGGAGCGG 
GTAAAGATCT TTCAGAGATT GATTTATGCA GATAAGCAAG AAGTTCAAGG AGATGGTCCA 
TTTCTGGATG GAATTAATGT CACAATAAGA AGGAATTATA TTTATGAAGA TGCTTATGAC 
AAACTTTCCC CTGAAAATGA GCCTGACTTG AAAAAGCGGA TCCGTGTGCA CTTACTGAAT 
GCGCACGGCC TGGACGAAGC CGGCATTGAC GGTGGTGGTA TTTTCAGAGA GTTTCTAAAC 
GAACTACTGA AGTCAGGGTT TAACCCCAAC CAGGGGTTCT TTAAGACCAC TAACGAAGGG 
CTTCTGTACC CCAACCCAGC CGCTCAGATG CTTGTGGGCG ATGCCTTTGC CAGACATTAC 
TACTTCCTGG GCAGGATGCT CGGAAAGGCC CTCTATGAAA ACATGCTGGT GGAGCTGCCC 
TTCGCCGGCT TCTTCCTGTC CAAGCTGCTG GGGACCAGCG CGGACGTGGA CATCCACCAC 
CTGGCGTCCT TAGACCCCGA GGTGTACAGG AACCTGCTCT TCCTCAAGAG CTATGAGGGC 
GACGTGGAGG AGCTGGGGCT GAACTTCACG GTGGTGAACA ACGACCTCGG AGAGGCCCAG 
GTAGTTGAAC TGAAATCTGG TGGGAAGGAC ATCCCGGTGA CCAGTGCCAA TCGCATTGCG 
TACATCCACC TGGTGGCCGA CTACCGGCTG AACCGGCAGA TCCGCCCGCA CTGCCTGGCG 
TTCCGGCAGG GCCTGGCCAA CGTGGTGAGC CTGGAGTGGC TGCGGATGTT CGACCAGCAG 
GAGATCCAGG TATTGATTTC TGGTGCACAA GTTCCCATAA GTCTGGAGGA CCTAAAATCC 
TTTACAAACT ATTCAGGAGG CTACTCTGCC GAGCACCCCG TGATCAAGGT CTTCTGGAGA 
GTCGTGGAGG GCTTCACGGA CGAGGAGAAG CGCAAACTGC TCAAGTTTGT GACCAGCTGC 
TCCCGGCCCC CCCTCTTGGG GTTTAAGGAG CTGTACCCCG CGTTCTGCAT CCACAACGGG 
GGCTCCGACC TGGAGCGGCT GCCCACAGCC AGCACCTGCA TGAACCTGCT GAAGCTCCCC 
GAGTTCCAGG ACGAGGCGCT GCTGCGGAGC AAGCTCCTCT ACGCCATCGA GTGTGCCGCG 
GGCTTCGAGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_005205800.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205857.1 Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

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CloneID	OBa18831
Clone ID Related Accession (Same CDS sequence)	XM_005205800.1
Accession Version	XM_005205800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin-protein ligase E3C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CTGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGGT CTGGAGTCTC CGCCGCTGTC CGTGTCTGAG 
GAGAGGCAGC TCGCCATCCT GACCCAGCTG CCCTTTGTGG TTCCGTTTGA GGAGCGGGTA 
AAGATCTTTC AGAGATTGAT TTATGCAGAT AAGCAAGAAG TTCAAGGAGA TGGTCCATTT 
CTGGATGGAA TTAATGTCAC AATAAGAAGG AATTATATTT ATGAAGATGC TTATGACAAA 
CTTTCCCCTG AAAATGAGCC TGACTTGAAA AAGCGGATCC GTGTGCACTT ACTGAATGCG 
CACGGCCTGG ACGAAGCCGG CATTGACGGT GGTGGTATTT TCAGAGAGTT TCTAAACGAA 
CTACTGAAGT CAGGGTTTAA CCCCAACCAG GGGTTCTTTA AGACCACTAA CGAAGGGCTT 
CTGTACCCCA ACCCAGCCGC TCAGATGCTT GTGGGCGATG CCTTTGCCAG ACATTACTAC 
TTCCTGGGCA GGATGCTCGG AAAGGCCCTC TATGAAAACA TGCTGGTGGA GCTGCCCTTC 
GCCGGCTTCT TCCTGTCCAA GCTGCTGGGG ACCAGCGCGG ACGTGGACAT CCACCACCTG 
GCGTCCTTAG ACCCCGAGGT GTACAGGAAC CTGCTCTTCC TCAAGAGCTA TGAGGGCGAC 
GTGGAGGAGC TGGGGCTGAA CTTCACGGTG GTGAACAACG ACCTCGGAGA GGCCCAGGTA 
GTTGAACTGA AATCTGGTGG GAAGGACATC CCGGTGACCA GTGCCAATCG CATTGCGTAC 
ATCCACCTGG TGGCCGACTA CCGGCTGAAC CGGCAGATCC GCCCGCACTG CCTGGCGTTC 
CGGCAGGGCC TGGCCAACGT GGTGAGCCTG GAGTGGCTGC GGATGTTCGA CCAGCAGGAG 
ATCCAGGTAT TGATTTCTGG TGCACAAGTT CCCATAAGTC TGGAGGACCT AAAATCCTTT 
ACAAACTATT CAGGAGGCTA CTCTGCCGAG CACCCCGTGA TCAAGGTCTT CTGGAGAGTC 
GTGGAGGGCT TCACGGACGA GGAGAAGCGC AAACTGCTCA AGTTTGTGAC CAGCTGCTCC 
CGGCCCCCCC TCTTGGGGTT TAAGGAGCTG TACCCCGCGT TCTGCATCCA CAACGGGGGC 
TCCGACCTGG AGCGGCTGCC CACAGCCAGC ACCTGCATGA ACCTGCTGAA GCTCCCCGAG 
TTCCAGGACG AGGCGCTGCT GCGGAGCAAG CTCCTCTACG CCATCGAGTG TGCCGCGGGC 
TTCGAGCTGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205857.1
CDS	256..3510
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQCSIQRSAFDRCASLSQSGGAFPLASGPNLTLLVRQLLFFYRQSEDSKRLIWMYQNLIKHSALFVQQLEGSERLTCLFQIKRLMSLCCRLLQSCNDDSLNVALPMRMLEVFSSEDTYLPVLQDASYVVSVIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTKEVLAAPFTEQIFHFVIPALADARTVFPYEPFLTALLSLEGRRPATSGGAPWLFYFVLTVGDNYLGALSEDGLLAHLRVLQALLSQLPVSPARASGQDAASDSDEEEGEEADRPASTPEDGRLSVLYIAEECLKKLDTKQQTNTLLSLVWRDSASEEAFTLMAAICHTLMVQHRVLVPRARLLYSLAFNARFLRHLWFLISSMTTRMITGSMVPLLQVISRGSPMSFEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELVVLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRNAWRFRRMGRIGPLRSTLDGLESPPLSVSEERQLAILTQLPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDAFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKSGGKDIPVTSANRIAYIHLVADYRLNRQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSAEHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFQDEALLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_005205800.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205800.1

ORF Insert Sequence:

ATGTTTAGTT TTGAGGGGGA CTTCAAAACC AGGCCCAAGG TGTCCCTGGG AGGCGCGAGC 
CGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG AAAGAGGGAG 
GAAGAAAGGC GAAGATTGAA AAATGCAATA ATTATCCAGT CATTTATTCG AGGCTATAGA 
GACAGAAAAC AGCAATGTTC TATCCAGAGG AGCGCATTCG ACCGCTGTGC CAGCCTGTCC 
CAGTCCGGTG GCGCTTTTCC CCTTGCCAGT GGTCCCAACC TTACCCTCCT GGTGCGGCAG 
CTCCTGTTTT TTTACAGACA GAGTGAAGAC TCGAAACGCT TGATATGGAT GTATCAGAAT 
CTGATTAAAC ACAGCGCTCT GTTTGTCCAG CAGTTGGAAG GATCTGAAAG ACTCACATGC 
TTGTTTCAAA TAAAGAGGCT GATGAGCCTC TGTTGCAGGT TGCTGCAGAG CTGTAATGAT 
GACAGTCTGA ATGTTGCGCT TCCCATGAGG ATGCTGGAGG TGTTCTCCTC TGAGGACACT 
TACTTGCCTG TTTTACAAGA TGCTAGCTAC GTGGTGTCCG TCATTGAACA AATTTTGCAC 
TACATGATTC AGAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AATATTCTGA TTTATCTCGA GTTCCTATAG CAAAAATTTT GCTAGAGAAT 
GTTTTAAAAC CATTGCACTT TACGTACAAC TCCTGTCCAG AAGGTGCAAG GCAACAGGTT 
TTTACAGCCT TCACGAAGGA GGTCCTGGCG GCCCCCTTCA CGGAGCAGAT CTTCCACTTC 
GTCATCCCTG CCCTGGCGGA CGCGCGGACC GTCTTCCCCT ACGAGCCCTT CCTGACTGCA 
CTGCTGTCCC TCGAGGGCAG ACGGCCCGCC ACCAGTGGGG GCGCACCATG GCTCTTCTAC 
TTTGTTTTAA CTGTTGGTGA CAATTATTTG GGCGCGCTCT CTGAGGACGG GCTGCTGGCG 
CACCTGCGAG TCCTCCAGGC CCTCCTGTCC CAGCTGCCCG TCTCGCCCGC CCGCGCCAGC 
GGCCAGGATG CAGCCAGCGA CTCGGATGAA GAGGAGGGGG AGGAGGCCGA CAGGCCGGCC 
AGCACTCCGG AGGATGGGCG GCTGTCTGTG CTGTACATCG CCGAGGAGTG TCTGAAGAAG 
CTGGACACGA AGCAGCAGAC CAACACGCTG CTGAGCCTGG TGTGGAGGGA CTCGGCCAGC 
GAGGAGGCCT TCACGCTGAT GGCGGCCATC TGCCACACGC TCATGGTGCA GCACCGCGTC 
TTGGTGCCAC GGGCCAGGCT TCTCTACAGT TTAGCCTTCA ATGCCAGGTT TCTGAGACAT 
CTCTGGTTTC TCATATCTTC AATGACAACG CGGATGATCA CAGGGTCTAT GGTACCATTG 
CTTCAGGTGA TATCAAGGGG CTCGCCTATG TCTTTTGAAG ATTCTAGTAG AATCATCCCA 
CTCTTCTACC TGTTTAGCTC CTTGTTCAGT CACTCACTGA TTTCCATACA TGATAACGAG 
TTCTTCGGAG ACCCCATAGA AGTTGTAGGT CAAAGACAAT CGTCAATGAT GCCTTTTACT 
TTAGAAGAAC TGGTAGTGTT GTCTCGATGC CTTCGCGACG CGTGCTTAGG GATCATCAAA 
CTGGCATATC CAGAAACAAA GCCAGAAGTT CGAGAAGAAT ATATCACCGC CTTTCAAAGT 
ATTGGAGTAA CTACCAGTTC TGAAATGCAG CAGTGTATAC AGATGGAGCA GAAACGATGG 
ATTCAGTTAT TTAAGGTCAT CACCAACCTG GTGAAGATGC TGAAGTCCAG GGACACGCGG 
AGGAACTTCT GCCCCCCCAA TCACTGGCTG TCAGAGCAGG AGGATATTAA AGCAGATAAG 
GTCACCCAGC TCTACGTCCC GGCGTCCAGG AACGCGTGGC GGTTCCGGCG GATGGGGAGG 
ATTGGGCCCC TGCGGTCCAC GTTGGACGGT CTGGAGTCTC CGCCGCTGTC CGTGTCTGAG 
GAGAGGCAGC TCGCCATCCT GACCCAGCTG CCCTTTGTGG TTCCGTTTGA GGAGCGGGTA 
AAGATCTTTC AGAGATTGAT TTATGCAGAT AAGCAAGAAG TTCAAGGAGA TGGTCCATTT 
CTGGATGGAA TTAATGTCAC AATAAGAAGG AATTATATTT ATGAAGATGC TTATGACAAA 
CTTTCCCCTG AAAATGAGCC TGACTTGAAA AAGCGGATCC GTGTGCACTT ACTGAATGCG 
CACGGCCTGG ACGAAGCCGG CATTGACGGT GGTGGTATTT TCAGAGAGTT TCTAAACGAA 
CTACTGAAGT CAGGGTTTAA CCCCAACCAG GGGTTCTTTA AGACCACTAA CGAAGGGCTT 
CTGTACCCCA ACCCAGCCGC TCAGATGCTT GTGGGCGATG CCTTTGCCAG ACATTACTAC 
TTCCTGGGCA GGATGCTCGG AAAGGCCCTC TATGAAAACA TGCTGGTGGA GCTGCCCTTC 
GCCGGCTTCT TCCTGTCCAA GCTGCTGGGG ACCAGCGCGG ACGTGGACAT CCACCACCTG 
GCGTCCTTAG ACCCCGAGGT GTACAGGAAC CTGCTCTTCC TCAAGAGCTA TGAGGGCGAC 
GTGGAGGAGC TGGGGCTGAA CTTCACGGTG GTGAACAACG ACCTCGGAGA GGCCCAGGTA 
GTTGAACTGA AATCTGGTGG GAAGGACATC CCGGTGACCA GTGCCAATCG CATTGCGTAC 
ATCCACCTGG TGGCCGACTA CCGGCTGAAC CGGCAGATCC GCCCGCACTG CCTGGCGTTC 
CGGCAGGGCC TGGCCAACGT GGTGAGCCTG GAGTGGCTGC GGATGTTCGA CCAGCAGGAG 
ATCCAGGTAT TGATTTCTGG TGCACAAGTT CCCATAAGTC TGGAGGACCT AAAATCCTTT 
ACAAACTATT CAGGAGGCTA CTCTGCCGAG CACCCCGTGA TCAAGGTCTT CTGGAGAGTC 
GTGGAGGGCT TCACGGACGA GGAGAAGCGC AAACTGCTCA AGTTTGTGAC CAGCTGCTCC 
CGGCCCCCCC TCTTGGGGTT TAAGGAGCTG TACCCCGCGT TCTGCATCCA CAACGGGGGC 
TCCGACCTGG AGCGGCTGCC CACAGCCAGC ACCTGCATGA ACCTGCTGAA GCTCCCCGAG 
TTCCAGGACG AGGCGCTGCT GCGGAGCAAG CTCCTCTACG CCATCGAGTG TGCCGCGGGC 
TTCGAGCTGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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UBE3C cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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