COL18A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL18A1
Entrez Gene ID	508076
Full Name	collagen type XVIII alpha 1 chain
General protein information	Preferred Names collagen type XVIII alpha 1 chain Names collagen alpha-1(XVIII) chain alpha 1 type XVIII collagen collagen, type XVIII, alpha 1 procollagen, type XVIII, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024975043.1	XP_024830811.1	collagen alpha-1(XVIII) chain isoform X2
NM_001083388.2	NP_001076857.1	collagen alpha-1(XVIII) chain precursor
XM_024975114.1	XP_024830882.1	collagen alpha-1(XVIII) chain isoform X4
XM_024975014.1	XP_024830782.1	collagen alpha-1(XVIII) chain isoform X1
XM_024975076.1	XP_024830844.1	collagen alpha-1(XVIII) chain isoform X3
XM_024975155.1	XP_024830923.1	collagen alpha-1(XVIII) chain isoform X5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Type XVIII collagen is newly transcribed during bovine adipogenesis.
Inoue-Murayama M, Sugimoto Y, Niimi Y, Aso H
Differentiation; research in biological diversity65(5)281-5(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL18A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL18A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa218675	XM_024975155.1 Latest version!	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa218671	XM_024975014.1 Latest version!	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218672	XM_024975043.1 Latest version!	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218673	XM_024975076.1 Latest version!	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218674	XM_024975114.1 Latest version!	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa38439	NM_001083388.2 Latest version!	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL18A1 ( XM_024975155.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830923.1 Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa218675
Clone ID Related Accession (Same CDS sequence)	XM_024975155.1
Accession Version	XM_024975155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVIII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGA GGTGGCCTTG GCTGCAGCCG CGGCGGCGTC GCCTCCTGGA CTTGCTCGCC 
CACCTGGTCC TGCTGCTCGG GGTCAGCGCA GCGTCCGCCG AGCCAGACGA AGAGGTCGTG 
GAGGTAGGGC TCCAGCAGCT GCTCGGGGAG CCCCCGCCCT GGCAGGTGGC CATGGTGCAC 
GACCCCGACG TGGGGTCCGC CTATGAGTTC GGCGAGGACG GCAGCGGTGG AGGCCAGGCG 
GCCCGGAGAC TCCTGCCTGG CACGTTCTTC CAGGACTTCT CGCTGCTTGT GCGTGTGCGG 
CCGGCCTCGG CGCGTGCGGG CGTGCTCTTT GCGGTCACAG ACCCGGCGCA GGCAGTGGTC 
TCGGTGGGCG TGAAGCTGAC GGCGGCGCGT GGGGGGCGGC AGCACATGCA GCTGCTGTAC 
ACGGAGCCGG GAGCCACCCA CACACGCACA GCCGCCAGCT TCGAGCTGCC CGCGCTGGAT 
GGTCGCTGGA CGCGCCTGGC ACTCAGCGTG GATGGCGCCT ATGCCAGGCT CTTCGTGGAC 
TGTAAGGAGG TCCAGGGCCA GCCCCTGGCG CACTCGCTGC ACGACCTGCA GCTGGAGCCG 
GACGCCCGCC TCTTCGTGGC CCAGGCCGGT GGAGCAGACC CTGAAAAGTT CCAGGGTCTG 
ATCTCAGAGC TGAGGTTGCG CAGGGATCCT CAGGTGAGCC CCCGGCAGTG CCAGGATGAG 
GACGAGGACG ACGATGATGA TGGGGCGTCC GGAGACCTGG GCAGCGGGCT CGTGGAGACC 
CAGGAGCATC TCCGGGAGGA GTTGGGTGCG CCCCTGGGGC CCAGGCTCCC TGAGGCTCCT 
CCGGTCACTT CTCCACCCTT GGCCGGAGTC GGCAACGAGG AGGACTTGAG AACTGAGGAA 
GTAGAGGAGT CAACCACAGC ATCTTCATTA GGAGCTCAGA CCCTCCCCAG CTTGGGCACA 
GTTGGCACGT GGGATGAGAG TGTTTGGAGC CCTGGGAGCA GCGTGAAAGA GCGAGGCCTG 
AAGGGGCAGA AGGGAGAGCC TGGCGCTCAG GGCCCCCCTG GCCCCATCGG CCCCCAGGGT 
CCTGCAGGCC CAGCCATAGA CAGCCATGAG GCACAGCCTG TTTCTGGACC ACAGGGACCC 
CCTGGGCCCC CGGGACCCCC GGGGAAGGAC GGCACCCCTG GAAGGGACGG TGAGCCTGGT 
GATCCTGGTG AAGACGGGAA GCCCGGGGAC ACTGGGCCAC AGGGCTTCCC AGGGACCCCA 
GGAGACGTGG GCCCCAAGGG CGAGAAGGGG GATCCTGGAG TCGGACCGAG GGGACCCCCA 
GGACCCCAGG GGCCTCCGGG GCCCCCAGGG CCCTCCTTCA AACCCGACAG GCTGACCTTC 
ATCGACATGG AGGGATCCGG TTTTGGCGGC GACCTGGAGA GCCTCCGCGG CCCTCGCGGC 
TTCCCCGGCC CCCCAGGGCC CCCTGGCGTC CCAGGCCTGC CAGGAGAGCC AGGCCGCTTC 
GGGATGAACA GCTCGGACGT CCCTGGACCC GCAGGCCTGC CCGGCGTGCC TGGGAGGGAC 
GGGCCGCCTG GGCGTCCAGG GCCCCCGGGA CCCCCAGGTA CTCCAGGAAA AGACGGACAA 
CCAGGACTGA CTGGACAGAA AGGCAGCCTC GGTGAGGCAG GCGCCCCAGG ACCCAAGGGA 
AGCAAGGGAG ACCTTGGCCC CGTCGGCGCT CCTGGGGAGC ATGGCCTGGC AGGAGCCCCT 
GGACCCGCAG GCCCACCAGG GCCCCCTGGC CCCCCGGGGC CCCCGGGCCC AGGATTCGCC 
GCAGGGTTTG ACGACATGGA AGGCTCCGGG AGCCCCTTAT GGTCAACAGC CCGAGGTGCC 
GAGGGGCTGC AGGGACCGCC TGGCACGCCC GGAGTCAAGG GGGATCCTGG AGCTGTGGGG 
CCACCAGGGA GCAAGGGAGA AGTTGGAGCA GACGGAGCAC CGGGCCTCCC CGGCCTCCCG 
GGCAGAGAGG GTGCAGCTGG ACCCCAGGGA CCAAAAGGAG AAAAAGGCAC TCAGGGAGAG 
AAAGGAGACC CAGGGAAGGA TGGAGTGGGG CAGCCAGGAC TCCCGGGACC CCCCGGACCC 
CCAGGGCCTG TCGTCTACGT GTCGGAGCAG GATAGAGTGC TGGCGAGCGT GCCAGGCCCC 
GAGGGCCGTG CAGGCTTCGC TGGCTTCCCC GGACCAGCCG GGCCAAAGGG AGACCTGGGC 
GCCAAAGGCC AGCAGGGCAT CCCGGGACCC AAGGGTGAGA AGGGAGAGCC GGGCACATTC 
TTCGGCCCCG ATGGCAGAGC ACTGACCCCC GCCCAGAAAG GAGCCAAGGG TGAGCCTGGC 
TTCCGAGGAC CTCCGGGTCC GTATGGGCGG CCCGGGCACA AGGGAGAGAT CGGATTCCCT 
GGACGGCCGG GGCGTCCCGG GATGAACGGG CTGAAAGGGG AGAAGGGGGA GCCGGGGGAC 
GCCAGCGTGG GATTCGGCAT GAGGGGTCCA GCTGGCCCAC CTGGGCCTCC GGGGCCCCCA 
GGCCCTCCCG GGACTCCTGT CTACGACAGC AACGCATTTG TGGACTCTGG CCGCCCTGGA 
CCCCCAGGAT TGCCAGGGCA CCAGGGGCCT TCTGGACTCA AGGGTGACAA AGGAGACGTG 
GGCCCGCCCG GACCGCCAGG GCAGTTCCCG TTTGACCTCC TCCAGCTGGG TGCCGAGATG 
AAGGGGGAGA AGGGGGACCG AGGGGCCGCT GGGCAGAAAG GAGAAAGGGG TGAGCCCGGG 
GGCGGAGGAT TCTTCGGCTC TGGTGTGCCT GGCCCCCCCG GCCCGCCTGG CTACCCAGGG 
ATTCCGGGCC CCAAGGGAGA GAGCATCCGC GGCCAGCCCG GCCCTCCGGG ACCTCAGGGA 
CCCCCTGGCA TTGGCTACGA GGGACCCCAG GGGCCTCCTG GGCCCCCCGG ACCCCCAGGG 
CCCCCGGGAC CCCCTTCCTT TCCTGGCCCC TACAGGCAGA CCATCAGTGT TCCTGGCCCG 
CCAGGCCCAC CTGGGCCCCC AGGACCCCCT GGATCCATGG GCACTTCTTC AGGGGTGAGG 
GTCTGGGCCA CGTACCAGAT GCTGGTGGAC CAGGTGCCGC AGGTGCCGGA GGGCTGGCTC 
ATCTACGTGG CTGACAGGGA GGAGCTGTAC GTCCGGGTCC GGAATGGGTT CCGGAAGGTT 
CTGCTGGAGG CCCACGTGCC CCTCCCCCAT GGGACGGACA ACGAGGTGGC CGTCTTGCAG 
CCCCCGCTGG TGCAGCTGCA TGAGGGTAAT CCCTACCCCC GCCGGGAGGT TCCACACCCG 
ACAGCGCGGT CCTGGCGGGC GGACGACATC CTGGCCAGTC CCCCGCGCCT GCCGCACCCC 
CAGCCCTACC CCGGCGCCCC GCACCACGGC GCCTACGCAC ACCTCCGGCC GGTGCCCCCC 
ACGGCCTCGC CCTCCCACAC CCACCACGAC TTCCAGCCGG TGCTGCACCT GGTGGCGCTC 
AACAGCCCGC AGTCGGGCGG CCTGCGCGGC ATCCGCGGCG CCGACTTTCA GTGCTTCCAG 
CAGGCGCGCG CCGCGGGGCT GGCCGGCACC TTCCGCGCGT TCCTGTCCTC GCGGTTGCAG 
GACCTGTACA GCATCGTGCG CCGCGCCGAC CGTGCCACCC TGCCCGTCGT CAACCTCAGG 
GACGAGGTGC TGTTTCCTAG CTGGGAGGCC TTGTTCTCAG GCTCCGAGGG CCAGCTGAAG 
CCCGGCGCCC GCATCTTCTC CTTCGACGGC AGAGATGTCC TTCAGCATCC CACCTGGCCC 
CAGAAGAGCG TGTGGCACGG CTCAGACCCC AGCGGGCGCC GGCTGACCGA GAGCTACTGC 
GAGACGTGGC GGACGGACAG CAGGGCGGCC ACGGGCCAGG CCTCCTCGCT GCTGGCGGGC 
CGGCTGCTGG AGCAGAAAGC CGCCGGGTGC CACAACGCCT TCATTGTCCT CTGCATCGAG 
AACAGCTTCA TGACCTCCTC CTCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830923.1
CDS	120..4109
Translation	MAPRWPWLQPRRRRLLDLLAHLVLLLGVSAASAEPDEEVVEVGLQQLLGEPPPWQVAMVHDPDVGSAYEFGEDGSGGGQAARRLLPGTFFQDFSLLVRVRPASARAGVLFAVTDPAQAVVSVGVKLTAARGGRQHMQLLYTEPGATHTRTAASFELPALDGRWTRLALSVDGAYARLFVDCKEVQGQPLAHSLHDLQLEPDARLFVAQAGGADPEKFQGLISELRLRRDPQVSPRQCQDEDEDDDDDGASGDLGSGLVETQEHLREELGAPLGPRLPEAPPVTSPPLAGVGNEEDLRTEEVEESTTASSLGAQTLPSLGTVGTWDESVWSPGSSVKERGLKGQKGEPGAQGPPGPIGPQGPAGPAIDSHEAQPVSGPQGPPGPPGPPGKDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGEKGDPGVGPRGPPGPQGPPGPPGPSFKPDRLTFIDMEGSGFGGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGMNSSDVPGPAGLPGVPGRDGPPGRPGPPGPPGTPGKDGQPGLTGQKGSLGEAGAPGPKGSKGDLGPVGAPGEHGLAGAPGPAGPPGPPGPPGPPGPGFAAGFDDMEGSGSPLWSTARGAEGLQGPPGTPGVKGDPGAVGPPGSKGEVGADGAPGLPGLPGREGAAGPQGPKGEKGTQGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDRVLASVPGPEGRAGFAGFPGPAGPKGDLGAKGQQGIPGPKGEKGEPGTFFGPDGRALTPAQKGAKGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGMNGLKGEKGEPGDASVGFGMRGPAGPPGPPGPPGPPGTPVYDSNAFVDSGRPGPPGLPGHQGPSGLKGDKGDVGPPGPPGQFPFDLLQLGAEMKGEKGDRGAAGQKGERGEPGGGGFFGSGVPGPPGPPGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGPQGPPGPPGPPGPPGPPSFPGPYRQTISVPGPPGPPGPPGPPGSMGTSSGVRVWATYQMLVDQVPQVPEGWLIYVADREELYVRVRNGFRKVLLEAHVPLPHGTDNEVAVLQPPLVQLHEGNPYPRREVPHPTARSWRADDILASPPRLPHPQPYPGAPHHGAYAHLRPVPPTASPSHTHHDFQPVLHLVALNSPQSGGLRGIRGADFQCFQQARAAGLAGTFRAFLSSRLQDLYSIVRRADRATLPVVNLRDEVLFPSWEALFSGSEGQLKPGARIFSFDGRDVLQHPTWPQKSVWHGSDPSGRRLTESYCETWRTDSRAATGQASSLLAGRLLEQKAAGCHNAFIVLCIENSFMTSSSK

Target ORF information:

RefSeq Version	XM_024975155.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975155.1

ORF Insert Sequence:

ATGGCGCCGA GGTGGCCTTG GCTGCAGCCG CGGCGGCGTC GCCTCCTGGA CTTGCTCGCC 
CACCTGGTCC TGCTGCTCGG GGTCAGCGCA GCGTCCGCCG AGCCAGACGA AGAGGTCGTG 
GAGGTAGGGC TCCAGCAGCT GCTCGGGGAG CCCCCGCCCT GGCAGGTGGC CATGGTGCAC 
GACCCCGACG TGGGGTCCGC CTATGAGTTC GGCGAGGACG GCAGCGGTGG AGGCCAGGCG 
GCCCGGAGAC TCCTGCCTGG CACGTTCTTC CAGGACTTCT CGCTGCTTGT GCGTGTGCGG 
CCGGCCTCGG CGCGTGCGGG CGTGCTCTTT GCGGTCACAG ACCCGGCGCA GGCAGTGGTC 
TCGGTGGGCG TGAAGCTGAC GGCGGCGCGT GGGGGGCGGC AGCACATGCA GCTGCTGTAC 
ACGGAGCCGG GAGCCACCCA CACACGCACA GCCGCCAGCT TCGAGCTGCC CGCGCTGGAT 
GGTCGCTGGA CGCGCCTGGC ACTCAGCGTG GATGGCGCCT ATGCCAGGCT CTTCGTGGAC 
TGTAAGGAGG TCCAGGGCCA GCCCCTGGCG CACTCGCTGC ACGACCTGCA GCTGGAGCCG 
GACGCCCGCC TCTTCGTGGC CCAGGCCGGT GGAGCAGACC CTGAAAAGTT CCAGGGTCTG 
ATCTCAGAGC TGAGGTTGCG CAGGGATCCT CAGGTGAGCC CCCGGCAGTG CCAGGATGAG 
GACGAGGACG ACGATGATGA TGGGGCGTCC GGAGACCTGG GCAGCGGGCT CGTGGAGACC 
CAGGAGCATC TCCGGGAGGA GTTGGGTGCG CCCCTGGGGC CCAGGCTCCC TGAGGCTCCT 
CCGGTCACTT CTCCACCCTT GGCCGGAGTC GGCAACGAGG AGGACTTGAG AACTGAGGAA 
GTAGAGGAGT CAACCACAGC ATCTTCATTA GGAGCTCAGA CCCTCCCCAG CTTGGGCACA 
GTTGGCACGT GGGATGAGAG TGTTTGGAGC CCTGGGAGCA GCGTGAAAGA GCGAGGCCTG 
AAGGGGCAGA AGGGAGAGCC TGGCGCTCAG GGCCCCCCTG GCCCCATCGG CCCCCAGGGT 
CCTGCAGGCC CAGCCATAGA CAGCCATGAG GCACAGCCTG TTTCTGGACC ACAGGGACCC 
CCTGGGCCCC CGGGACCCCC GGGGAAGGAC GGCACCCCTG GAAGGGACGG TGAGCCTGGT 
GATCCTGGTG AAGACGGGAA GCCCGGGGAC ACTGGGCCAC AGGGCTTCCC AGGGACCCCA 
GGAGACGTGG GCCCCAAGGG CGAGAAGGGG GATCCTGGAG TCGGACCGAG GGGACCCCCA 
GGACCCCAGG GGCCTCCGGG GCCCCCAGGG CCCTCCTTCA AACCCGACAG GCTGACCTTC 
ATCGACATGG AGGGATCCGG TTTTGGCGGC GACCTGGAGA GCCTCCGCGG CCCTCGCGGC 
TTCCCCGGCC CCCCAGGGCC CCCTGGCGTC CCAGGCCTGC CAGGAGAGCC AGGCCGCTTC 
GGGATGAACA GCTCGGACGT CCCTGGACCC GCAGGCCTGC CCGGCGTGCC TGGGAGGGAC 
GGGCCGCCTG GGCGTCCAGG GCCCCCGGGA CCCCCAGGTA CTCCAGGAAA AGACGGACAA 
CCAGGACTGA CTGGACAGAA AGGCAGCCTC GGTGAGGCAG GCGCCCCAGG ACCCAAGGGA 
AGCAAGGGAG ACCTTGGCCC CGTCGGCGCT CCTGGGGAGC ATGGCCTGGC AGGAGCCCCT 
GGACCCGCAG GCCCACCAGG GCCCCCTGGC CCCCCGGGGC CCCCGGGCCC AGGATTCGCC 
GCAGGGTTTG ACGACATGGA AGGCTCCGGG AGCCCCTTAT GGTCAACAGC CCGAGGTGCC 
GAGGGGCTGC AGGGACCGCC TGGCACGCCC GGAGTCAAGG GGGATCCTGG AGCTGTGGGG 
CCACCAGGGA GCAAGGGAGA AGTTGGAGCA GACGGAGCAC CGGGCCTCCC CGGCCTCCCG 
GGCAGAGAGG GTGCAGCTGG ACCCCAGGGA CCAAAAGGAG AAAAAGGCAC TCAGGGAGAG 
AAAGGAGACC CAGGGAAGGA TGGAGTGGGG CAGCCAGGAC TCCCGGGACC CCCCGGACCC 
CCAGGGCCTG TCGTCTACGT GTCGGAGCAG GATAGAGTGC TGGCGAGCGT GCCAGGCCCC 
GAGGGCCGTG CAGGCTTCGC TGGCTTCCCC GGACCAGCCG GGCCAAAGGG AGACCTGGGC 
GCCAAAGGCC AGCAGGGCAT CCCGGGACCC AAGGGTGAGA AGGGAGAGCC GGGCACATTC 
TTCGGCCCCG ATGGCAGAGC ACTGACCCCC GCCCAGAAAG GAGCCAAGGG TGAGCCTGGC 
TTCCGAGGAC CTCCGGGTCC GTATGGGCGG CCCGGGCACA AGGGAGAGAT CGGATTCCCT 
GGACGGCCGG GGCGTCCCGG GATGAACGGG CTGAAAGGGG AGAAGGGGGA GCCGGGGGAC 
GCCAGCGTGG GATTCGGCAT GAGGGGTCCA GCTGGCCCAC CTGGGCCTCC GGGGCCCCCA 
GGCCCTCCCG GGACTCCTGT CTACGACAGC AACGCATTTG TGGACTCTGG CCGCCCTGGA 
CCCCCAGGAT TGCCAGGGCA CCAGGGGCCT TCTGGACTCA AGGGTGACAA AGGAGACGTG 
GGCCCGCCCG GACCGCCAGG GCAGTTCCCG TTTGACCTCC TCCAGCTGGG TGCCGAGATG 
AAGGGGGAGA AGGGGGACCG AGGGGCCGCT GGGCAGAAAG GAGAAAGGGG TGAGCCCGGG 
GGCGGAGGAT TCTTCGGCTC TGGTGTGCCT GGCCCCCCCG GCCCGCCTGG CTACCCAGGG 
ATTCCGGGCC CCAAGGGAGA GAGCATCCGC GGCCAGCCCG GCCCTCCGGG ACCTCAGGGA 
CCCCCTGGCA TTGGCTACGA GGGACCCCAG GGGCCTCCTG GGCCCCCCGG ACCCCCAGGG 
CCCCCGGGAC CCCCTTCCTT TCCTGGCCCC TACAGGCAGA CCATCAGTGT TCCTGGCCCG 
CCAGGCCCAC CTGGGCCCCC AGGACCCCCT GGATCCATGG GCACTTCTTC AGGGGTGAGG 
GTCTGGGCCA CGTACCAGAT GCTGGTGGAC CAGGTGCCGC AGGTGCCGGA GGGCTGGCTC 
ATCTACGTGG CTGACAGGGA GGAGCTGTAC GTCCGGGTCC GGAATGGGTT CCGGAAGGTT 
CTGCTGGAGG CCCACGTGCC CCTCCCCCAT GGGACGGACA ACGAGGTGGC CGTCTTGCAG 
CCCCCGCTGG TGCAGCTGCA TGAGGGTAAT CCCTACCCCC GCCGGGAGGT TCCACACCCG 
ACAGCGCGGT CCTGGCGGGC GGACGACATC CTGGCCAGTC CCCCGCGCCT GCCGCACCCC 
CAGCCCTACC CCGGCGCCCC GCACCACGGC GCCTACGCAC ACCTCCGGCC GGTGCCCCCC 
ACGGCCTCGC CCTCCCACAC CCACCACGAC TTCCAGCCGG TGCTGCACCT GGTGGCGCTC 
AACAGCCCGC AGTCGGGCGG CCTGCGCGGC ATCCGCGGCG CCGACTTTCA GTGCTTCCAG 
CAGGCGCGCG CCGCGGGGCT GGCCGGCACC TTCCGCGCGT TCCTGTCCTC GCGGTTGCAG 
GACCTGTACA GCATCGTGCG CCGCGCCGAC CGTGCCACCC TGCCCGTCGT CAACCTCAGG 
GACGAGGTGC TGTTTCCTAG CTGGGAGGCC TTGTTCTCAG GCTCCGAGGG CCAGCTGAAG 
CCCGGCGCCC GCATCTTCTC CTTCGACGGC AGAGATGTCC TTCAGCATCC CACCTGGCCC 
CAGAAGAGCG TGTGGCACGG CTCAGACCCC AGCGGGCGCC GGCTGACCGA GAGCTACTGC 
GAGACGTGGC GGACGGACAG CAGGGCGGCC ACGGGCCAGG CCTCCTCGCT GCTGGCGGGC 
CGGCTGCTGG AGCAGAAAGC CGCCGGGTGC CACAACGCCT TCATTGTCCT CTGCATCGAG 
AACAGCTTCA TGACCTCCTC CTCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( XM_024975014.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830782.1 Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa218671
Clone ID Related Accession (Same CDS sequence)	XM_024975014.1
Accession Version	XM_024975014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GGTAGATCTT CTCTTTCCTC CGTGCCGGCC CCGGCGCCTC CCCGGGGAAG CCAGCTGCCT 
CTCGGGCGGC CCCTGCAGCT GGACGGTGAA GGACTCTTGC TTGCAGCAGC CCGTCCCGGC 
CAGCAGCATG CTGACGACCG CAGGCCCATG CCGCCTCTGC CTCCCCTGGT GACGGGTTCC 
CTGGGCAAGC ACACTGCCCT CTGGGCTCTC TCCTCTGACC CCCCTGCCAA ACTGTCTCAT 
GTCGCAGTCT CAGCTCTAAC TGTGGACAGT GGTGCTTGGG CTCCCCACGT GGCTAATTCT 
GCGGGGCCTG GTCTTGCTAA CAAGTCCACA CTGCTCGGGG CTGCTGAGCT GGCTGCCACC 
GAGCAGCCTG TCCCCACCAC CGCTGGCCGC TGCCTGCCCC TGCTGCCCTC CCTGGCTCTC 
TGCGGCCACC TGGGCATCAG ACGCTCCTGG TGGCCCAACC ACCTCCACCA CACAAGTCGG 
GAGGAGGTGC AGGCCGCGGT CCCAGCCTGG GGGGCACTGC TCCGGACCCA CTGCCACCGC 
TTCCTGGCCT GGTTCTTCTG CCTGCTGCTG GCACCCCGCT GCGGGCCCGG CCTGCCGCCC 
GGCCTGCCAC CCTGTCGCCA GTTCTGCGAG GCCCTGGAGG ACGCATGCTG GAGCCACCTG 
GACGGGCGCG GGCTGCCACT CCCCTGCGCC TCGCTCCCGG CCCGCGAGGA CGGGCACTGC 
GTGTTCATAG GGCCACCGGC AGACGAAGAG GTCGTGGAGG TAGGGCTCCA GCAGCTGCTC 
GGGGAGCCCC CGCCCTGGCA GGTGGCCATG GTGCACGACC CCGACGTGGG GTCCGCCTAT 
GAGTTCGGCG AGGACGGCAG CGGTGGAGGC CAGGCGGCCC GGAGACTCCT GCCTGGCACG 
TTCTTCCAGG ACTTCTCGCT GCTTGTGCGT GTGCGGCCGG CCTCGGCGCG TGCGGGCGTG 
CTCTTTGCGG TCACAGACCC GGCGCAGGCA GTGGTCTCGG TGGGCGTGAA GCTGACGGCG 
GCGCGTGGGG GGCGGCAGCA CATGCAGCTG CTGTACACGG AGCCGGGAGC CACCCACACA 
CGCACAGCCG CCAGCTTCGA GCTGCCCGCG CTGGATGGTC GCTGGACGCG CCTGGCACTC 
AGCGTGGATG GCGCCTATGC CAGGCTCTTC GTGGACTGTA AGGAGGTCCA GGGCCAGCCC 
CTGGCGCACT CGCTGCACGA CCTGCAGCTG GAGCCGGACG CCCGCCTCTT CGTGGCCCAG 
GCCGGTGGAG CAGACCCTGA AAAGTTCCAG GGTCTGATCT CAGAGCTGAG GTTGCGCAGG 
GATCCTCAGG TGAGCCCCCG GCAGTGCCAG GATGAGGACG AGGACGACGA TGATGATGGG 
GCGTCCGGAG ACCTGGGCAG CGGGCTCGTG GAGACCCAGG AGCATCTCCG GGAGGAGTTG 
GGTGCGCCCC TGGGGCCCAG GCTCCCTGAG GCTCCTCCGG TCACTTCTCC ACCCTTGGCC 
GGAGTCGGCA ACGAGGAGGA CTTGAGAACT GAGGAAGTAG AGGAGTCAAC CACAGCATCT 
TCATTAGGAG CTCAGACCCT CCCCAGCTTG GGCACAGTTG GCACGTGGGA TGAGAGTGTT 
TGGAGCCCTG GGAGCAGCGT GAAAGAGCGA GGCCTGAAGG GGCAGAAGGG AGAGCCTGGC 
GCTCAGGGCC CCCCTGGCCC CATCGGCCCC CAGGGTCCTG CAGGCCCAGC CATAGACAGC 
CATGAGGCAC AGCCTGTTTC TGGACCACAG GGACCCCCTG GGCCCCCGGG ACCCCCGGGG 
AAGGACGGCA CCCCTGGAAG GGACGGTGAG CCTGGTGATC CTGGTGAAGA CGGGAAGCCC 
GGGGACACTG GGCCACAGGG CTTCCCAGGG ACCCCAGGAG ACGTGGGCCC CAAGGGCGAG 
AAGGGGGATC CTGGAGTCGG ACCGAGGGGA CCCCCAGGAC CCCAGGGGCC TCCGGGGCCC 
CCAGGGCCCT CCTTCAAACC CGACAGGCTG ACCTTCATCG ACATGGAGGG ATCCGGTTTT 
GGCGGCGACC TGGAGAGCCT CCGCGGCCCT CGCGGCTTCC CCGGCCCCCC AGGGCCCCCT 
GGCGTCCCAG GCCTGCCAGG AGAGCCAGGC CGCTTCGGGA TGAACAGCTC GGACGTCCCT 
GGACCCGCAG GCCTGCCCGG CGTGCCTGGG AGGGACGGGC CGCCTGGGCG TCCAGGGCCC 
CCGGGACCCC CAGGTACTCC AGGAAAAGAC GGACAACCAG GACTGACTGG ACAGAAAGGC 
AGCCTCGGTG AGGCAGGCGC CCCAGGACCC AAGGGAAGCA AGGGAGACCT TGGCCCCGTC 
GGCGCTCCTG GGGAGCATGG CCTGGCAGGA GCCCCTGGAC CCGCAGGCCC ACCAGGGCCC 
CCTGGCCCCC CGGGGCCCCC GGGCCCAGGA TTCGCCGCAG GGTTTGACGA CATGGAAGGC 
TCCGGGAGCC CCTTATGGTC AACAGCCCGA GGTGCCGAGG GGCTGCAGGG ACCGCCTGGC 
ACGCCCGGAG TCAAGGGGGA TCCTGGAGCT GTGGGGCCAC CAGGGAGCAA GGGAGAAGTT 
GGAGCAGACG GAGCACCGGG CCTCCCCGGC CTCCCGGGCA GAGAGGGTGC AGCTGGACCC 
CAGGGACCAA AAGGAGAAAA AGGCACTCAG GGAGAGAAAG GAGACCCAGG GAAGGATGGA 
GTGGGGCAGC CAGGACTCCC GGGACCCCCC GGACCCCCAG GGCCTGTCGT CTACGTGTCG 
GAGCAGGATA GAGTGCTGGC GAGCGTGCCA GGCCCCGAGG GCCGTGCAGG CTTCGCTGGC 
TTCCCCGGAC CAGCCGGGCC AAAGGGAGAC CTGGGCGCCA AAGGCCAGCA GGGCATCCCG 
GGACCCAAGG GTGAGAAGGG AGAGCCGGGC ACATTCTTCG GCCCCGATGG CAGAGCACTG 
ACCCCCGCCC AGAAAGGAGC CAAGGGTGAG CCTGGCTTCC GAGGACCTCC GGGTCCGTAT 
GGGCGGCCCG GGCACAAGGG AGAGATCGGA TTCCCTGGAC GGCCGGGGCG TCCCGGGATG 
AACGGGCTGA AAGGGGAGAA GGGGGAGCCG GGGGACGCCA GCGTGGGATT CGGCATGAGG 
GGTCCAGCTG GCCCACCTGG GCCTCCGGGG CCCCCAGGCC CTCCCGGGAC TCCTGTCTAC 
GACAGCAACG CATTTGTGGA CTCTGGCCGC CCTGGACCCC CAGGATTGCC AGGGCACCAG 
GGGCCTTCTG GACTCAAGGG TGACAAAGGA GACGTGGGCC CGCCCGGACC GCCAGGGCAG 
TTCCCGTTTG ACCTCCTCCA GCTGGGTGCC GAGATGAAGG GGGAGAAGGG GGACCGAGGG 
GCCGCTGGGC AGAAAGGAGA AAGGGGTGAG CCCGGGGGCG GAGGATTCTT CGGCTCTGGT 
GTGCCTGGCC CCCCCGGCCC GCCTGGCTAC CCAGGGATTC CGGGCCCCAA GGGAGAGAGC 
ATCCGCGGCC AGCCCGGCCC TCCGGGACCT CAGGGACCCC CTGGCATTGG CTACGAGGGA 
CCCCAGGGGC CTCCTGGGCC CCCCGGACCC CCAGGGCCCC CGGGACCCCC TTCCTTTCCT 
GGCCCCTACA GGCAGACCAT CAGTGTTCCT GGCCCGCCAG GCCCACCTGG GCCCCCAGGA 
CCCCCTGGAT CCATGGGCAC TTCTTCAGGG GGATCCACTG GGCTGGGCTT CCAGCAGGTG 
AGGGTCTGGG CCACGTACCA GATGCTGGTG GACCAGGTGC CGCAGGTGCC GGAGGGCTGG 
CTCATCTACG TGGCTGACAG GGAGGAGCTG TACGTCCGGG TCCGGAATGG GTTCCGGAAG 
GTTCTGCTGG AGGCCCACGT GCCCCTCCCC CATGGGACGG ACAACGAGGT GGCCGTCTTG 
CAGCCCCCGC TGGTGCAGCT GCATGAGGGT AATCCCTACC CCCGCCGGGA GGTTCCACAC 
CCGACAGCGC GGTCCTGGCG GGCGGACGAC ATCCTGGCCA GTCCCCCGCG CCTGCCGCAC 
CCCCAGCCCT ACCCCGGCGC CCCGCACCAC GGCGCCTACG CACACCTCCG GCCGGTGCCC 
CCCACGGCCT CGCCCTCCCA CACCCACCAC GACTTCCAGC CGGTGCTGCA CCTGGTGGCG 
CTCAACAGCC CGCAGTCGGG CGGCCTGCGC GGCATCCGCG GCGCCGACTT TCAGTGCTTC 
CAGCAGGCGC GCGCCGCGGG GCTGGCCGGC ACCTTCCGCG CGTTCCTGTC CTCGCGGTTG 
CAGGACCTGT ACAGCATCGT GCGCCGCGCC GACCGTGCCA CCCTGCCCGT CGTCAACCTC 
AGGGACGAGG TGCTGTTTCC TAGCTGGGAG GCCTTGTTCT CAGGCTCCGA GGGCCAGCTG 
AAGCCCGGCG CCCGCATCTT CTCCTTCGAC GGCAGAGATG TCCTTCAGCA TCCCACCTGG 
CCCCAGAAGA GCGTGTGGCA CGGCTCAGAC CCCAGCGGGC GCCGGCTGAC CGAGAGCTAC 
TGCGAGACGT GGCGGACGGA CAGCAGGGCG GCCACGGGCC AGGCCTCCTC GCTGCTGGCG 
GGCCGGCTGC TGGAGCAGAA AGCCGCCGGG TGCCACAACG CCTTCATTGT CCTCTGCATC 
GAGAACAGCT TCATGACCTC CTCCTCCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830782.1
CDS	37..5349
Translation	MAPDPSSGRGHHGLLLLACCLSAAQADLFSWIWSGNSAWPIAAPASKPPDSSPVWPTEDTTTHVAPRDAPTDWWKVSASPTAPLGRPEVGQGQTPTGPTASTVSLDPKEENIAGVGAKILNVAQGIRSFVQLWDNAKDTTPAESSAGAETPPPTDTMDDLSTPGPSITPQDSGTTLQLSTTALSSPDTQRTEAGTLPMPTQPPPSPGRPWFRESLVSPTPGRSSLSSVPAPAPPRGSQLPLGRPLQLDGEGLLLAAARPGQQHADDRRPMPPLPPLVTGSLGKHTALWALSSDPPAKLSHVAVSALTVDSGAWAPHVANSAGPGLANKSTLLGAAELAATEQPVPTTAGRCLPLLPSLALCGHLGIRRSWWPNHLHHTSREEVQAAVPAWGALLRTHCHRFLAWFFCLLLAPRCGPGLPPGLPPCRQFCEALEDACWSHLDGRGLPLPCASLPAREDGHCVFIGPPADEEVVEVGLQQLLGEPPPWQVAMVHDPDVGSAYEFGEDGSGGGQAARRLLPGTFFQDFSLLVRVRPASARAGVLFAVTDPAQAVVSVGVKLTAARGGRQHMQLLYTEPGATHTRTAASFELPALDGRWTRLALSVDGAYARLFVDCKEVQGQPLAHSLHDLQLEPDARLFVAQAGGADPEKFQGLISELRLRRDPQVSPRQCQDEDEDDDDDGASGDLGSGLVETQEHLREELGAPLGPRLPEAPPVTSPPLAGVGNEEDLRTEEVEESTTASSLGAQTLPSLGTVGTWDESVWSPGSSVKERGLKGQKGEPGAQGPPGPIGPQGPAGPAIDSHEAQPVSGPQGPPGPPGPPGKDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGEKGDPGVGPRGPPGPQGPPGPPGPSFKPDRLTFIDMEGSGFGGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGMNSSDVPGPAGLPGVPGRDGPPGRPGPPGPPGTPGKDGQPGLTGQKGSLGEAGAPGPKGSKGDLGPVGAPGEHGLAGAPGPAGPPGPPGPPGPPGPGFAAGFDDMEGSGSPLWSTARGAEGLQGPPGTPGVKGDPGAVGPPGSKGEVGADGAPGLPGLPGREGAAGPQGPKGEKGTQGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDRVLASVPGPEGRAGFAGFPGPAGPKGDLGAKGQQGIPGPKGEKGEPGTFFGPDGRALTPAQKGAKGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGMNGLKGEKGEPGDASVGFGMRGPAGPPGPPGPPGPPGTPVYDSNAFVDSGRPGPPGLPGHQGPSGLKGDKGDVGPPGPPGQFPFDLLQLGAEMKGEKGDRGAAGQKGERGEPGGGGFFGSGVPGPPGPPGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGPQGPPGPPGPPGPPGPPSFPGPYRQTISVPGPPGPPGPPGPPGSMGTSSGGSTGLGFQQVRVWATYQMLVDQVPQVPEGWLIYVADREELYVRVRNGFRKVLLEAHVPLPHGTDNEVAVLQPPLVQLHEGNPYPRREVPHPTARSWRADDILASPPRLPHPQPYPGAPHHGAYAHLRPVPPTASPSHTHHDFQPVLHLVALNSPQSGGLRGIRGADFQCFQQARAAGLAGTFRAFLSSRLQDLYSIVRRADRATLPVVNLRDEVLFPSWEALFSGSEGQLKPGARIFSFDGRDVLQHPTWPQKSVWHGSDPSGRRLTESYCETWRTDSRAATGQASSLLAGRLLEQKAAGCHNAFIVLCIENSFMTSSSK

Target ORF information:

RefSeq Version	XM_024975014.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975014.1

ORF Insert Sequence:

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GGTAGATCTT CTCTTTCCTC CGTGCCGGCC CCGGCGCCTC CCCGGGGAAG CCAGCTGCCT 
CTCGGGCGGC CCCTGCAGCT GGACGGTGAA GGACTCTTGC TTGCAGCAGC CCGTCCCGGC 
CAGCAGCATG CTGACGACCG CAGGCCCATG CCGCCTCTGC CTCCCCTGGT GACGGGTTCC 
CTGGGCAAGC ACACTGCCCT CTGGGCTCTC TCCTCTGACC CCCCTGCCAA ACTGTCTCAT 
GTCGCAGTCT CAGCTCTAAC TGTGGACAGT GGTGCTTGGG CTCCCCACGT GGCTAATTCT 
GCGGGGCCTG GTCTTGCTAA CAAGTCCACA CTGCTCGGGG CTGCTGAGCT GGCTGCCACC 
GAGCAGCCTG TCCCCACCAC CGCTGGCCGC TGCCTGCCCC TGCTGCCCTC CCTGGCTCTC 
TGCGGCCACC TGGGCATCAG ACGCTCCTGG TGGCCCAACC ACCTCCACCA CACAAGTCGG 
GAGGAGGTGC AGGCCGCGGT CCCAGCCTGG GGGGCACTGC TCCGGACCCA CTGCCACCGC 
TTCCTGGCCT GGTTCTTCTG CCTGCTGCTG GCACCCCGCT GCGGGCCCGG CCTGCCGCCC 
GGCCTGCCAC CCTGTCGCCA GTTCTGCGAG GCCCTGGAGG ACGCATGCTG GAGCCACCTG 
GACGGGCGCG GGCTGCCACT CCCCTGCGCC TCGCTCCCGG CCCGCGAGGA CGGGCACTGC 
GTGTTCATAG GGCCACCGGC AGACGAAGAG GTCGTGGAGG TAGGGCTCCA GCAGCTGCTC 
GGGGAGCCCC CGCCCTGGCA GGTGGCCATG GTGCACGACC CCGACGTGGG GTCCGCCTAT 
GAGTTCGGCG AGGACGGCAG CGGTGGAGGC CAGGCGGCCC GGAGACTCCT GCCTGGCACG 
TTCTTCCAGG ACTTCTCGCT GCTTGTGCGT GTGCGGCCGG CCTCGGCGCG TGCGGGCGTG 
CTCTTTGCGG TCACAGACCC GGCGCAGGCA GTGGTCTCGG TGGGCGTGAA GCTGACGGCG 
GCGCGTGGGG GGCGGCAGCA CATGCAGCTG CTGTACACGG AGCCGGGAGC CACCCACACA 
CGCACAGCCG CCAGCTTCGA GCTGCCCGCG CTGGATGGTC GCTGGACGCG CCTGGCACTC 
AGCGTGGATG GCGCCTATGC CAGGCTCTTC GTGGACTGTA AGGAGGTCCA GGGCCAGCCC 
CTGGCGCACT CGCTGCACGA CCTGCAGCTG GAGCCGGACG CCCGCCTCTT CGTGGCCCAG 
GCCGGTGGAG CAGACCCTGA AAAGTTCCAG GGTCTGATCT CAGAGCTGAG GTTGCGCAGG 
GATCCTCAGG TGAGCCCCCG GCAGTGCCAG GATGAGGACG AGGACGACGA TGATGATGGG 
GCGTCCGGAG ACCTGGGCAG CGGGCTCGTG GAGACCCAGG AGCATCTCCG GGAGGAGTTG 
GGTGCGCCCC TGGGGCCCAG GCTCCCTGAG GCTCCTCCGG TCACTTCTCC ACCCTTGGCC 
GGAGTCGGCA ACGAGGAGGA CTTGAGAACT GAGGAAGTAG AGGAGTCAAC CACAGCATCT 
TCATTAGGAG CTCAGACCCT CCCCAGCTTG GGCACAGTTG GCACGTGGGA TGAGAGTGTT 
TGGAGCCCTG GGAGCAGCGT GAAAGAGCGA GGCCTGAAGG GGCAGAAGGG AGAGCCTGGC 
GCTCAGGGCC CCCCTGGCCC CATCGGCCCC CAGGGTCCTG CAGGCCCAGC CATAGACAGC 
CATGAGGCAC AGCCTGTTTC TGGACCACAG GGACCCCCTG GGCCCCCGGG ACCCCCGGGG 
AAGGACGGCA CCCCTGGAAG GGACGGTGAG CCTGGTGATC CTGGTGAAGA CGGGAAGCCC 
GGGGACACTG GGCCACAGGG CTTCCCAGGG ACCCCAGGAG ACGTGGGCCC CAAGGGCGAG 
AAGGGGGATC CTGGAGTCGG ACCGAGGGGA CCCCCAGGAC CCCAGGGGCC TCCGGGGCCC 
CCAGGGCCCT CCTTCAAACC CGACAGGCTG ACCTTCATCG ACATGGAGGG ATCCGGTTTT 
GGCGGCGACC TGGAGAGCCT CCGCGGCCCT CGCGGCTTCC CCGGCCCCCC AGGGCCCCCT 
GGCGTCCCAG GCCTGCCAGG AGAGCCAGGC CGCTTCGGGA TGAACAGCTC GGACGTCCCT 
GGACCCGCAG GCCTGCCCGG CGTGCCTGGG AGGGACGGGC CGCCTGGGCG TCCAGGGCCC 
CCGGGACCCC CAGGTACTCC AGGAAAAGAC GGACAACCAG GACTGACTGG ACAGAAAGGC 
AGCCTCGGTG AGGCAGGCGC CCCAGGACCC AAGGGAAGCA AGGGAGACCT TGGCCCCGTC 
GGCGCTCCTG GGGAGCATGG CCTGGCAGGA GCCCCTGGAC CCGCAGGCCC ACCAGGGCCC 
CCTGGCCCCC CGGGGCCCCC GGGCCCAGGA TTCGCCGCAG GGTTTGACGA CATGGAAGGC 
TCCGGGAGCC CCTTATGGTC AACAGCCCGA GGTGCCGAGG GGCTGCAGGG ACCGCCTGGC 
ACGCCCGGAG TCAAGGGGGA TCCTGGAGCT GTGGGGCCAC CAGGGAGCAA GGGAGAAGTT 
GGAGCAGACG GAGCACCGGG CCTCCCCGGC CTCCCGGGCA GAGAGGGTGC AGCTGGACCC 
CAGGGACCAA AAGGAGAAAA AGGCACTCAG GGAGAGAAAG GAGACCCAGG GAAGGATGGA 
GTGGGGCAGC CAGGACTCCC GGGACCCCCC GGACCCCCAG GGCCTGTCGT CTACGTGTCG 
GAGCAGGATA GAGTGCTGGC GAGCGTGCCA GGCCCCGAGG GCCGTGCAGG CTTCGCTGGC 
TTCCCCGGAC CAGCCGGGCC AAAGGGAGAC CTGGGCGCCA AAGGCCAGCA GGGCATCCCG 
GGACCCAAGG GTGAGAAGGG AGAGCCGGGC ACATTCTTCG GCCCCGATGG CAGAGCACTG 
ACCCCCGCCC AGAAAGGAGC CAAGGGTGAG CCTGGCTTCC GAGGACCTCC GGGTCCGTAT 
GGGCGGCCCG GGCACAAGGG AGAGATCGGA TTCCCTGGAC GGCCGGGGCG TCCCGGGATG 
AACGGGCTGA AAGGGGAGAA GGGGGAGCCG GGGGACGCCA GCGTGGGATT CGGCATGAGG 
GGTCCAGCTG GCCCACCTGG GCCTCCGGGG CCCCCAGGCC CTCCCGGGAC TCCTGTCTAC 
GACAGCAACG CATTTGTGGA CTCTGGCCGC CCTGGACCCC CAGGATTGCC AGGGCACCAG 
GGGCCTTCTG GACTCAAGGG TGACAAAGGA GACGTGGGCC CGCCCGGACC GCCAGGGCAG 
TTCCCGTTTG ACCTCCTCCA GCTGGGTGCC GAGATGAAGG GGGAGAAGGG GGACCGAGGG 
GCCGCTGGGC AGAAAGGAGA AAGGGGTGAG CCCGGGGGCG GAGGATTCTT CGGCTCTGGT 
GTGCCTGGCC CCCCCGGCCC GCCTGGCTAC CCAGGGATTC CGGGCCCCAA GGGAGAGAGC 
ATCCGCGGCC AGCCCGGCCC TCCGGGACCT CAGGGACCCC CTGGCATTGG CTACGAGGGA 
CCCCAGGGGC CTCCTGGGCC CCCCGGACCC CCAGGGCCCC CGGGACCCCC TTCCTTTCCT 
GGCCCCTACA GGCAGACCAT CAGTGTTCCT GGCCCGCCAG GCCCACCTGG GCCCCCAGGA 
CCCCCTGGAT CCATGGGCAC TTCTTCAGGG GGATCCACTG GGCTGGGCTT CCAGCAGGTG 
AGGGTCTGGG CCACGTACCA GATGCTGGTG GACCAGGTGC CGCAGGTGCC GGAGGGCTGG 
CTCATCTACG TGGCTGACAG GGAGGAGCTG TACGTCCGGG TCCGGAATGG GTTCCGGAAG 
GTTCTGCTGG AGGCCCACGT GCCCCTCCCC CATGGGACGG ACAACGAGGT GGCCGTCTTG 
CAGCCCCCGC TGGTGCAGCT GCATGAGGGT AATCCCTACC CCCGCCGGGA GGTTCCACAC 
CCGACAGCGC GGTCCTGGCG GGCGGACGAC ATCCTGGCCA GTCCCCCGCG CCTGCCGCAC 
CCCCAGCCCT ACCCCGGCGC CCCGCACCAC GGCGCCTACG CACACCTCCG GCCGGTGCCC 
CCCACGGCCT CGCCCTCCCA CACCCACCAC GACTTCCAGC CGGTGCTGCA CCTGGTGGCG 
CTCAACAGCC CGCAGTCGGG CGGCCTGCGC GGCATCCGCG GCGCCGACTT TCAGTGCTTC 
CAGCAGGCGC GCGCCGCGGG GCTGGCCGGC ACCTTCCGCG CGTTCCTGTC CTCGCGGTTG 
CAGGACCTGT ACAGCATCGT GCGCCGCGCC GACCGTGCCA CCCTGCCCGT CGTCAACCTC 
AGGGACGAGG TGCTGTTTCC TAGCTGGGAG GCCTTGTTCT CAGGCTCCGA GGGCCAGCTG 
AAGCCCGGCG CCCGCATCTT CTCCTTCGAC GGCAGAGATG TCCTTCAGCA TCCCACCTGG 
CCCCAGAAGA GCGTGTGGCA CGGCTCAGAC CCCAGCGGGC GCCGGCTGAC CGAGAGCTAC 
TGCGAGACGT GGCGGACGGA CAGCAGGGCG GCCACGGGCC AGGCCTCCTC GCTGCTGGCG 
GGCCGGCTGC TGGAGCAGAA AGCCGCCGGG TGCCACAACG CCTTCATTGT CCTCTGCATC 
GAGAACAGCT TCATGACCTC CTCCTCCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( XM_024975043.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830811.1 Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X2, mRNA.

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CloneID	OBa218672
Clone ID Related Accession (Same CDS sequence)	XM_024975043.1
Accession Version	XM_024975043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GGTAGATCTT CTCTTTCCTC CGTGCCGGCC CCGGCGCCTC CCCGGGGAAG CCAGCTGCCT 
CTCGGGCGGC CCCTGCAGCT GGACGGTGAA GGACTCTTGC TTGCAGCAGC CCGTCCCGGC 
CAGCAGCATG CTGACGACCG CAGGCCCATG CCGCCTCTGC CTCCCCTGGT GACGGGTTCC 
CTGGGCAAGC ACACTGCCCT CTGGGCTCTC TCCTCTGACC CCCCTGCCAA ACTGTCTCAT 
GTCGCAGTCT CAGCTCTAAC TGTGGACAGT GGTGCTTGGG CTCCCCACGT GGCTAATTCT 
GCGGGGCCTG GTCTTGCTAA CAAGTCCACA CTGCTCGGGG CTGCTGAGCT GGCTGCCACC 
GAGCAGCCTG TCCCCACCAC CGCTGGCCGC TGCCTGCCCC TGCTGCCCTC CCTGGCTCTC 
TGCGGCCACC TGGGCATCAG ACGCTCCTGG TGGCCCAACC ACCTCCACCA CACAAGTCGG 
GAGGAGGTGC AGGCCGCGGT CCCAGCCTGG GGGGCACTGC TCCGGACCCA CTGCCACCGC 
TTCCTGGCCT GGTTCTTCTG CCTGCTGCTG GCACCCCGCT GCGGGCCCGG CCTGCCGCCC 
GGCCTGCCAC CCTGTCGCCA GTTCTGCGAG GCCCTGGAGG ACGCATGCTG GAGCCACCTG 
GACGGGCGCG GGCTGCCACT CCCCTGCGCC TCGCTCCCGG CCCGCGAGGA CGGGCACTGC 
GTGTTCATAG GGCCACCGGC AGACGAAGAG GTCGTGGAGG TAGGGCTCCA GCAGCTGCTC 
GGGGAGCCCC CGCCCTGGCA GGTGGCCATG GTGCACGACC CCGACGTGGG GTCCGCCTAT 
GAGTTCGGCG AGGACGGCAG CGGTGGAGGC CAGGCGGCCC GGAGACTCCT GCCTGGCACG 
TTCTTCCAGG ACTTCTCGCT GCTTGTGCGT GTGCGGCCGG CCTCGGCGCG TGCGGGCGTG 
CTCTTTGCGG TCACAGACCC GGCGCAGGCA GTGGTCTCGG TGGGCGTGAA GCTGACGGCG 
GCGCGTGGGG GGCGGCAGCA CATGCAGCTG CTGTACACGG AGCCGGGAGC CACCCACACA 
CGCACAGCCG CCAGCTTCGA GCTGCCCGCG CTGGATGGTC GCTGGACGCG CCTGGCACTC 
AGCGTGGATG GCGCCTATGC CAGGCTCTTC GTGGACTGTA AGGAGGTCCA GGGCCAGCCC 
CTGGCGCACT CGCTGCACGA CCTGCAGCTG GAGCCGGACG CCCGCCTCTT CGTGGCCCAG 
GCCGGTGGAG CAGACCCTGA AAAGTTCCAG GGTCTGATCT CAGAGCTGAG GTTGCGCAGG 
GATCCTCAGG TGAGCCCCCG GCAGTGCCAG GATGAGGACG AGGACGACGA TGATGATGGG 
GCGTCCGGAG ACCTGGGCAG CGGGCTCGTG GAGACCCAGG AGCATCTCCG GGAGGAGTTG 
GGTGCGCCCC TGGGGCCCAG GCTCCCTGAG GCTCCTCCGG TCACTTCTCC ACCCTTGGCC 
GGAGTCGGCA ACGAGGAGGA CTTGAGAACT GAGGAAGTAG AGGAGTCAAC CACAGCATCT 
TCATTAGGAG CTCAGACCCT CCCCAGCTTG GGCACAGTTG GCACGTGGGA TGAGAGTGTT 
TGGAGCCCTG GGAGCAGCGT GAAAGAGCGA GGCCTGAAGG GGCAGAAGGG AGAGCCTGGC 
GCTCAGGGCC CCCCTGGCCC CATCGGCCCC CAGGGTCCTG CAGGCCCAGC CATAGACAGC 
CATGAGGCAC AGCCTGTTTC TGGACCACAG GGACCCCCTG GGCCCCCGGG ACCCCCGGGG 
AAGGACGGCA CCCCTGGAAG GGACGGTGAG CCTGGTGATC CTGGTGAAGA CGGGAAGCCC 
GGGGACACTG GGCCACAGGG CTTCCCAGGG ACCCCAGGAG ACGTGGGCCC CAAGGGCGAG 
AAGGGGGATC CTGGAGTCGG ACCGAGGGGA CCCCCAGGAC CCCAGGGGCC TCCGGGGCCC 
CCAGGGCCCT CCTTCAAACC CGACAGGCTG ACCTTCATCG ACATGGAGGG ATCCGGTTTT 
GGCGGCGACC TGGAGAGCCT CCGCGGCCCT CGCGGCTTCC CCGGCCCCCC AGGGCCCCCT 
GGCGTCCCAG GCCTGCCAGG AGAGCCAGGC CGCTTCGGGA TGAACAGCTC GGACGTCCCT 
GGACCCGCAG GCCTGCCCGG CGTGCCTGGG AGGGACGGGC CGCCTGGGCG TCCAGGGCCC 
CCGGGACCCC CAGGTACTCC AGGAAAAGAC GGACAACCAG GACTGACTGG ACAGAAAGGC 
AGCCTCGGTG AGGCAGGCGC CCCAGGACCC AAGGGAAGCA AGGGAGACCT TGGCCCCGTC 
GGCGCTCCTG GGGAGCATGG CCTGGCAGGA GCCCCTGGAC CCGCAGGCCC ACCAGGGCCC 
CCTGGCCCCC CGGGGCCCCC GGGCCCAGGA TTCGCCGCAG GGTTTGACGA CATGGAAGGC 
TCCGGGAGCC CCTTATGGTC AACAGCCCGA GGTGCCGAGG GGCTGCAGGG ACCGCCTGGC 
ACGCCCGGAG TCAAGGGGGA TCCTGGAGCT GTGGGGCCAC CAGGGAGCAA GGGAGAAGTT 
GGAGCAGACG GAGCACCGGG CCTCCCCGGC CTCCCGGGCA GAGAGGGTGC AGCTGGACCC 
CAGGGACCAA AAGGAGAAAA AGGCACTCAG GGAGAGAAAG GAGACCCAGG GAAGGATGGA 
GTGGGGCAGC CAGGACTCCC GGGACCCCCC GGACCCCCAG GGCCTGTCGT CTACGTGTCG 
GAGCAGGATA GAGTGCTGGC GAGCGTGCCA GGCCCCGAGG GCCGTGCAGG CTTCGCTGGC 
TTCCCCGGAC CAGCCGGGCC AAAGGGAGAC CTGGGCGCCA AAGGCCAGCA GGGCATCCCG 
GGACCCAAGG GTGAGAAGGG AGAGCCGGGC ACATTCTTCG GCCCCGATGG CAGAGCACTG 
ACCCCCGCCC AGAAAGGAGC CAAGGGTGAG CCTGGCTTCC GAGGACCTCC GGGTCCGTAT 
GGGCGGCCCG GGCACAAGGG AGAGATCGGA TTCCCTGGAC GGCCGGGGCG TCCCGGGATG 
AACGGGCTGA AAGGGGAGAA GGGGGAGCCG GGGGACGCCA GCGTGGGATT CGGCATGAGG 
GGTCCAGCTG GCCCACCTGG GCCTCCGGGG CCCCCAGGCC CTCCCGGGAC TCCTGTCTAC 
GACAGCAACG CATTTGTGGA CTCTGGCCGC CCTGGACCCC CAGGATTGCC AGGGCACCAG 
GGGCCTTCTG GACTCAAGGG TGACAAAGGA GACGTGGGCC CGCCCGGACC GCCAGGGCAG 
TTCCCGTTTG ACCTCCTCCA GCTGGGTGCC GAGATGAAGG GGGAGAAGGG GGACCGAGGG 
GCCGCTGGGC AGAAAGGAGA AAGGGGTGAG CCCGGGGGCG GAGGATTCTT CGGCTCTGGT 
GTGCCTGGCC CCCCCGGCCC GCCTGGCTAC CCAGGGATTC CGGGCCCCAA GGGAGAGAGC 
ATCCGCGGCC AGCCCGGCCC TCCGGGACCT CAGGGACCCC CTGGCATTGG CTACGAGGGA 
CCCCAGGGGC CTCCTGGGCC CCCCGGACCC CCAGGGCCCC CGGGACCCCC TTCCTTTCCT 
GGCCCCTACA GGCAGACCAT CAGTGTTCCT GGCCCGCCAG GCCCACCTGG GCCCCCAGGA 
CCCCCTGGAT CCATGGGCAC TTCTTCAGGG CAGGTGAGGG TCTGGGCCAC GTACCAGATG 
CTGGTGGACC AGGTGCCGCA GGTGCCGGAG GGCTGGCTCA TCTACGTGGC TGACAGGGAG 
GAGCTGTACG TCCGGGTCCG GAATGGGTTC CGGAAGGTTC TGCTGGAGGC CCACGTGCCC 
CTCCCCCATG GGACGGACAA CGAGGTGGCC GTCTTGCAGC CCCCGCTGGT GCAGCTGCAT 
GAGGGTAATC CCTACCCCCG CCGGGAGGTT CCACACCCGA CAGCGCGGTC CTGGCGGGCG 
GACGACATCC TGGCCAGTCC CCCGCGCCTG CCGCACCCCC AGCCCTACCC CGGCGCCCCG 
CACCACGGCG CCTACGCACA CCTCCGGCCG GTGCCCCCCA CGGCCTCGCC CTCCCACACC 
CACCACGACT TCCAGCCGGT GCTGCACCTG GTGGCGCTCA ACAGCCCGCA GTCGGGCGGC 
CTGCGCGGCA TCCGCGGCGC CGACTTTCAG TGCTTCCAGC AGGCGCGCGC CGCGGGGCTG 
GCCGGCACCT TCCGCGCGTT CCTGTCCTCG CGGTTGCAGG ACCTGTACAG CATCGTGCGC 
CGCGCCGACC GTGCCACCCT GCCCGTCGTC AACCTCAGGG ACGAGGTGCT GTTTCCTAGC 
TGGGAGGCCT TGTTCTCAGG CTCCGAGGGC CAGCTGAAGC CCGGCGCCCG CATCTTCTCC 
TTCGACGGCA GAGATGTCCT TCAGCATCCC ACCTGGCCCC AGAAGAGCGT GTGGCACGGC 
TCAGACCCCA GCGGGCGCCG GCTGACCGAG AGCTACTGCG AGACGTGGCG GACGGACAGC 
AGGGCGGCCA CGGGCCAGGC CTCCTCGCTG CTGGCGGGCC GGCTGCTGGA GCAGAAAGCC 
GCCGGGTGCC ACAACGCCTT CATTGTCCTC TGCATCGAGA ACAGCTTCAT GACCTCCTCC 
TCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830811.1
CDS	37..5325
Translation	MAPDPSSGRGHHGLLLLACCLSAAQADLFSWIWSGNSAWPIAAPASKPPDSSPVWPTEDTTTHVAPRDAPTDWWKVSASPTAPLGRPEVGQGQTPTGPTASTVSLDPKEENIAGVGAKILNVAQGIRSFVQLWDNAKDTTPAESSAGAETPPPTDTMDDLSTPGPSITPQDSGTTLQLSTTALSSPDTQRTEAGTLPMPTQPPPSPGRPWFRESLVSPTPGRSSLSSVPAPAPPRGSQLPLGRPLQLDGEGLLLAAARPGQQHADDRRPMPPLPPLVTGSLGKHTALWALSSDPPAKLSHVAVSALTVDSGAWAPHVANSAGPGLANKSTLLGAAELAATEQPVPTTAGRCLPLLPSLALCGHLGIRRSWWPNHLHHTSREEVQAAVPAWGALLRTHCHRFLAWFFCLLLAPRCGPGLPPGLPPCRQFCEALEDACWSHLDGRGLPLPCASLPAREDGHCVFIGPPADEEVVEVGLQQLLGEPPPWQVAMVHDPDVGSAYEFGEDGSGGGQAARRLLPGTFFQDFSLLVRVRPASARAGVLFAVTDPAQAVVSVGVKLTAARGGRQHMQLLYTEPGATHTRTAASFELPALDGRWTRLALSVDGAYARLFVDCKEVQGQPLAHSLHDLQLEPDARLFVAQAGGADPEKFQGLISELRLRRDPQVSPRQCQDEDEDDDDDGASGDLGSGLVETQEHLREELGAPLGPRLPEAPPVTSPPLAGVGNEEDLRTEEVEESTTASSLGAQTLPSLGTVGTWDESVWSPGSSVKERGLKGQKGEPGAQGPPGPIGPQGPAGPAIDSHEAQPVSGPQGPPGPPGPPGKDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGEKGDPGVGPRGPPGPQGPPGPPGPSFKPDRLTFIDMEGSGFGGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGMNSSDVPGPAGLPGVPGRDGPPGRPGPPGPPGTPGKDGQPGLTGQKGSLGEAGAPGPKGSKGDLGPVGAPGEHGLAGAPGPAGPPGPPGPPGPPGPGFAAGFDDMEGSGSPLWSTARGAEGLQGPPGTPGVKGDPGAVGPPGSKGEVGADGAPGLPGLPGREGAAGPQGPKGEKGTQGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDRVLASVPGPEGRAGFAGFPGPAGPKGDLGAKGQQGIPGPKGEKGEPGTFFGPDGRALTPAQKGAKGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGMNGLKGEKGEPGDASVGFGMRGPAGPPGPPGPPGPPGTPVYDSNAFVDSGRPGPPGLPGHQGPSGLKGDKGDVGPPGPPGQFPFDLLQLGAEMKGEKGDRGAAGQKGERGEPGGGGFFGSGVPGPPGPPGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGPQGPPGPPGPPGPPGPPSFPGPYRQTISVPGPPGPPGPPGPPGSMGTSSGQVRVWATYQMLVDQVPQVPEGWLIYVADREELYVRVRNGFRKVLLEAHVPLPHGTDNEVAVLQPPLVQLHEGNPYPRREVPHPTARSWRADDILASPPRLPHPQPYPGAPHHGAYAHLRPVPPTASPSHTHHDFQPVLHLVALNSPQSGGLRGIRGADFQCFQQARAAGLAGTFRAFLSSRLQDLYSIVRRADRATLPVVNLRDEVLFPSWEALFSGSEGQLKPGARIFSFDGRDVLQHPTWPQKSVWHGSDPSGRRLTESYCETWRTDSRAATGQASSLLAGRLLEQKAAGCHNAFIVLCIENSFMTSSSK

Target ORF information:

RefSeq Version	XM_024975043.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975043.1

ORF Insert Sequence:

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GGTAGATCTT CTCTTTCCTC CGTGCCGGCC CCGGCGCCTC CCCGGGGAAG CCAGCTGCCT 
CTCGGGCGGC CCCTGCAGCT GGACGGTGAA GGACTCTTGC TTGCAGCAGC CCGTCCCGGC 
CAGCAGCATG CTGACGACCG CAGGCCCATG CCGCCTCTGC CTCCCCTGGT GACGGGTTCC 
CTGGGCAAGC ACACTGCCCT CTGGGCTCTC TCCTCTGACC CCCCTGCCAA ACTGTCTCAT 
GTCGCAGTCT CAGCTCTAAC TGTGGACAGT GGTGCTTGGG CTCCCCACGT GGCTAATTCT 
GCGGGGCCTG GTCTTGCTAA CAAGTCCACA CTGCTCGGGG CTGCTGAGCT GGCTGCCACC 
GAGCAGCCTG TCCCCACCAC CGCTGGCCGC TGCCTGCCCC TGCTGCCCTC CCTGGCTCTC 
TGCGGCCACC TGGGCATCAG ACGCTCCTGG TGGCCCAACC ACCTCCACCA CACAAGTCGG 
GAGGAGGTGC AGGCCGCGGT CCCAGCCTGG GGGGCACTGC TCCGGACCCA CTGCCACCGC 
TTCCTGGCCT GGTTCTTCTG CCTGCTGCTG GCACCCCGCT GCGGGCCCGG CCTGCCGCCC 
GGCCTGCCAC CCTGTCGCCA GTTCTGCGAG GCCCTGGAGG ACGCATGCTG GAGCCACCTG 
GACGGGCGCG GGCTGCCACT CCCCTGCGCC TCGCTCCCGG CCCGCGAGGA CGGGCACTGC 
GTGTTCATAG GGCCACCGGC AGACGAAGAG GTCGTGGAGG TAGGGCTCCA GCAGCTGCTC 
GGGGAGCCCC CGCCCTGGCA GGTGGCCATG GTGCACGACC CCGACGTGGG GTCCGCCTAT 
GAGTTCGGCG AGGACGGCAG CGGTGGAGGC CAGGCGGCCC GGAGACTCCT GCCTGGCACG 
TTCTTCCAGG ACTTCTCGCT GCTTGTGCGT GTGCGGCCGG CCTCGGCGCG TGCGGGCGTG 
CTCTTTGCGG TCACAGACCC GGCGCAGGCA GTGGTCTCGG TGGGCGTGAA GCTGACGGCG 
GCGCGTGGGG GGCGGCAGCA CATGCAGCTG CTGTACACGG AGCCGGGAGC CACCCACACA 
CGCACAGCCG CCAGCTTCGA GCTGCCCGCG CTGGATGGTC GCTGGACGCG CCTGGCACTC 
AGCGTGGATG GCGCCTATGC CAGGCTCTTC GTGGACTGTA AGGAGGTCCA GGGCCAGCCC 
CTGGCGCACT CGCTGCACGA CCTGCAGCTG GAGCCGGACG CCCGCCTCTT CGTGGCCCAG 
GCCGGTGGAG CAGACCCTGA AAAGTTCCAG GGTCTGATCT CAGAGCTGAG GTTGCGCAGG 
GATCCTCAGG TGAGCCCCCG GCAGTGCCAG GATGAGGACG AGGACGACGA TGATGATGGG 
GCGTCCGGAG ACCTGGGCAG CGGGCTCGTG GAGACCCAGG AGCATCTCCG GGAGGAGTTG 
GGTGCGCCCC TGGGGCCCAG GCTCCCTGAG GCTCCTCCGG TCACTTCTCC ACCCTTGGCC 
GGAGTCGGCA ACGAGGAGGA CTTGAGAACT GAGGAAGTAG AGGAGTCAAC CACAGCATCT 
TCATTAGGAG CTCAGACCCT CCCCAGCTTG GGCACAGTTG GCACGTGGGA TGAGAGTGTT 
TGGAGCCCTG GGAGCAGCGT GAAAGAGCGA GGCCTGAAGG GGCAGAAGGG AGAGCCTGGC 
GCTCAGGGCC CCCCTGGCCC CATCGGCCCC CAGGGTCCTG CAGGCCCAGC CATAGACAGC 
CATGAGGCAC AGCCTGTTTC TGGACCACAG GGACCCCCTG GGCCCCCGGG ACCCCCGGGG 
AAGGACGGCA CCCCTGGAAG GGACGGTGAG CCTGGTGATC CTGGTGAAGA CGGGAAGCCC 
GGGGACACTG GGCCACAGGG CTTCCCAGGG ACCCCAGGAG ACGTGGGCCC CAAGGGCGAG 
AAGGGGGATC CTGGAGTCGG ACCGAGGGGA CCCCCAGGAC CCCAGGGGCC TCCGGGGCCC 
CCAGGGCCCT CCTTCAAACC CGACAGGCTG ACCTTCATCG ACATGGAGGG ATCCGGTTTT 
GGCGGCGACC TGGAGAGCCT CCGCGGCCCT CGCGGCTTCC CCGGCCCCCC AGGGCCCCCT 
GGCGTCCCAG GCCTGCCAGG AGAGCCAGGC CGCTTCGGGA TGAACAGCTC GGACGTCCCT 
GGACCCGCAG GCCTGCCCGG CGTGCCTGGG AGGGACGGGC CGCCTGGGCG TCCAGGGCCC 
CCGGGACCCC CAGGTACTCC AGGAAAAGAC GGACAACCAG GACTGACTGG ACAGAAAGGC 
AGCCTCGGTG AGGCAGGCGC CCCAGGACCC AAGGGAAGCA AGGGAGACCT TGGCCCCGTC 
GGCGCTCCTG GGGAGCATGG CCTGGCAGGA GCCCCTGGAC CCGCAGGCCC ACCAGGGCCC 
CCTGGCCCCC CGGGGCCCCC GGGCCCAGGA TTCGCCGCAG GGTTTGACGA CATGGAAGGC 
TCCGGGAGCC CCTTATGGTC AACAGCCCGA GGTGCCGAGG GGCTGCAGGG ACCGCCTGGC 
ACGCCCGGAG TCAAGGGGGA TCCTGGAGCT GTGGGGCCAC CAGGGAGCAA GGGAGAAGTT 
GGAGCAGACG GAGCACCGGG CCTCCCCGGC CTCCCGGGCA GAGAGGGTGC AGCTGGACCC 
CAGGGACCAA AAGGAGAAAA AGGCACTCAG GGAGAGAAAG GAGACCCAGG GAAGGATGGA 
GTGGGGCAGC CAGGACTCCC GGGACCCCCC GGACCCCCAG GGCCTGTCGT CTACGTGTCG 
GAGCAGGATA GAGTGCTGGC GAGCGTGCCA GGCCCCGAGG GCCGTGCAGG CTTCGCTGGC 
TTCCCCGGAC CAGCCGGGCC AAAGGGAGAC CTGGGCGCCA AAGGCCAGCA GGGCATCCCG 
GGACCCAAGG GTGAGAAGGG AGAGCCGGGC ACATTCTTCG GCCCCGATGG CAGAGCACTG 
ACCCCCGCCC AGAAAGGAGC CAAGGGTGAG CCTGGCTTCC GAGGACCTCC GGGTCCGTAT 
GGGCGGCCCG GGCACAAGGG AGAGATCGGA TTCCCTGGAC GGCCGGGGCG TCCCGGGATG 
AACGGGCTGA AAGGGGAGAA GGGGGAGCCG GGGGACGCCA GCGTGGGATT CGGCATGAGG 
GGTCCAGCTG GCCCACCTGG GCCTCCGGGG CCCCCAGGCC CTCCCGGGAC TCCTGTCTAC 
GACAGCAACG CATTTGTGGA CTCTGGCCGC CCTGGACCCC CAGGATTGCC AGGGCACCAG 
GGGCCTTCTG GACTCAAGGG TGACAAAGGA GACGTGGGCC CGCCCGGACC GCCAGGGCAG 
TTCCCGTTTG ACCTCCTCCA GCTGGGTGCC GAGATGAAGG GGGAGAAGGG GGACCGAGGG 
GCCGCTGGGC AGAAAGGAGA AAGGGGTGAG CCCGGGGGCG GAGGATTCTT CGGCTCTGGT 
GTGCCTGGCC CCCCCGGCCC GCCTGGCTAC CCAGGGATTC CGGGCCCCAA GGGAGAGAGC 
ATCCGCGGCC AGCCCGGCCC TCCGGGACCT CAGGGACCCC CTGGCATTGG CTACGAGGGA 
CCCCAGGGGC CTCCTGGGCC CCCCGGACCC CCAGGGCCCC CGGGACCCCC TTCCTTTCCT 
GGCCCCTACA GGCAGACCAT CAGTGTTCCT GGCCCGCCAG GCCCACCTGG GCCCCCAGGA 
CCCCCTGGAT CCATGGGCAC TTCTTCAGGG CAGGTGAGGG TCTGGGCCAC GTACCAGATG 
CTGGTGGACC AGGTGCCGCA GGTGCCGGAG GGCTGGCTCA TCTACGTGGC TGACAGGGAG 
GAGCTGTACG TCCGGGTCCG GAATGGGTTC CGGAAGGTTC TGCTGGAGGC CCACGTGCCC 
CTCCCCCATG GGACGGACAA CGAGGTGGCC GTCTTGCAGC CCCCGCTGGT GCAGCTGCAT 
GAGGGTAATC CCTACCCCCG CCGGGAGGTT CCACACCCGA CAGCGCGGTC CTGGCGGGCG 
GACGACATCC TGGCCAGTCC CCCGCGCCTG CCGCACCCCC AGCCCTACCC CGGCGCCCCG 
CACCACGGCG CCTACGCACA CCTCCGGCCG GTGCCCCCCA CGGCCTCGCC CTCCCACACC 
CACCACGACT TCCAGCCGGT GCTGCACCTG GTGGCGCTCA ACAGCCCGCA GTCGGGCGGC 
CTGCGCGGCA TCCGCGGCGC CGACTTTCAG TGCTTCCAGC AGGCGCGCGC CGCGGGGCTG 
GCCGGCACCT TCCGCGCGTT CCTGTCCTCG CGGTTGCAGG ACCTGTACAG CATCGTGCGC 
CGCGCCGACC GTGCCACCCT GCCCGTCGTC AACCTCAGGG ACGAGGTGCT GTTTCCTAGC 
TGGGAGGCCT TGTTCTCAGG CTCCGAGGGC CAGCTGAAGC CCGGCGCCCG CATCTTCTCC 
TTCGACGGCA GAGATGTCCT TCAGCATCCC ACCTGGCCCC AGAAGAGCGT GTGGCACGGC 
TCAGACCCCA GCGGGCGCCG GCTGACCGAG AGCTACTGCG AGACGTGGCG GACGGACAGC 
AGGGCGGCCA CGGGCCAGGC CTCCTCGCTG CTGGCGGGCC GGCTGCTGGA GCAGAAAGCC 
GCCGGGTGCC ACAACGCCTT CATTGTCCTC TGCATCGAGA ACAGCTTCAT GACCTCCTCC 
TCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( XM_024975076.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830844.1 Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X3, mRNA.

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CloneID	OBa218673
Clone ID Related Accession (Same CDS sequence)	XM_024975076.1
Accession Version	XM_024975076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVIII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GGTAGATCTT CTCTTTCCTC CGTGCCGGCC CCGGCGCCTC CCCGGGGAAG CCAGCTGCCT 
CTCGGGCGGC CCCTGCAGCT GGACGGTGAA GGACTCTTGC TTGCAGCAGC CCGTCCCGGC 
CAGCAGCATG CTGACGACCG CAGGCCCATG CCGCCTCTGC CTCCCCTGGT GACGGGTTCC 
CTGGGCAAGC ACACTGCCCT CTGGGCTCTC TCCTCTGACC CCCCTGCCAA ACTGTCTCAT 
GTCGCAGTCT CAGCTCTAAC TGTGGACAGT GGTGCTTGGG CTCCCCACGT GGCTAATTCT 
GCGGGGCCTG GTCTTGCTAA CAAGTCCACA CTGCTCGGGG CTGCTGAGCT GGCTGCCACC 
GAGCAGCCTG TCCCCACCAC CGCTGGCCGC TGCCTGCCCC TGCTGCCCTC CCTGGCTCTC 
TGCGGCCACC TGGGCATCAG ACGCTCCTGG TGGCCCAACC ACCTCCACCA CACAAGTCGG 
GAGGAGGTGC AGGCCGCGGT CCCAGCCTGG GGGGCACTGC TCCGGACCCA CTGCCACCGC 
TTCCTGGCCT GGTTCTTCTG CCTGCTGCTG GCACCCCGCT GCGGGCCCGG CCTGCCGCCC 
GGCCTGCCAC CCTGTCGCCA GTTCTGCGAG GCCCTGGAGG ACGCATGCTG GAGCCACCTG 
GACGGGCGCG GGCTGCCACT CCCCTGCGCC TCGCTCCCGG CCCGCGAGGA CGGGCACTGC 
GTGTTCATAG GGCCACCGGC AGACGAAGAG GTCGTGGAGG TAGGGCTCCA GCAGCTGCTC 
GGGGAGCCCC CGCCCTGGCA GGTGGCCATG GTGCACGACC CCGACGTGGG GTCCGCCTAT 
GAGTTCGGCG AGGACGGCAG CGGTGGAGGC CAGGCGGCCC GGAGACTCCT GCCTGGCACG 
TTCTTCCAGG ACTTCTCGCT GCTTGTGCGT GTGCGGCCGG CCTCGGCGCG TGCGGGCGTG 
CTCTTTGCGG TCACAGACCC GGCGCAGGCA GTGGTCTCGG TGGGCGTGAA GCTGACGGCG 
GCGCGTGGGG GGCGGCAGCA CATGCAGCTG CTGTACACGG AGCCGGGAGC CACCCACACA 
CGCACAGCCG CCAGCTTCGA GCTGCCCGCG CTGGATGGTC GCTGGACGCG CCTGGCACTC 
AGCGTGGATG GCGCCTATGC CAGGCTCTTC GTGGACTGTA AGGAGGTCCA GGGCCAGCCC 
CTGGCGCACT CGCTGCACGA CCTGCAGCTG GAGCCGGACG CCCGCCTCTT CGTGGCCCAG 
GCCGGTGGAG CAGACCCTGA AAAGTTCCAG GGTCTGATCT CAGAGCTGAG GTTGCGCAGG 
GATCCTCAGG TGAGCCCCCG GCAGTGCCAG GATGAGGACG AGGACGACGA TGATGATGGG 
GCGTCCGGAG ACCTGGGCAG CGGGCTCGTG GAGACCCAGG AGCATCTCCG GGAGGAGTTG 
GGTGCGCCCC TGGGGCCCAG GCTCCCTGAG GCTCCTCCGG TCACTTCTCC ACCCTTGGCC 
GGAGTCGGCA ACGAGGAGGA CTTGAGAACT GAGGAAGTAG AGGAGTCAAC CACAGCATCT 
TCATTAGGAG CTCAGACCCT CCCCAGCTTG GGCACAGTTG GCACGTGGGA TGAGAGTGTT 
TGGAGCCCTG GGAGCAGCGT GAAAGAGCGA GGCCTGAAGG GGCAGAAGGG AGAGCCTGGC 
GCTCAGGGCC CCCCTGGCCC CATCGGCCCC CAGGGTCCTG CAGGCCCAGC CATAGACAGC 
CATGAGGCAC AGCCTGTTTC TGGACCACAG GGACCCCCTG GGCCCCCGGG ACCCCCGGGG 
AAGGACGGCA CCCCTGGAAG GGACGGTGAG CCTGGTGATC CTGGTGAAGA CGGGAAGCCC 
GGGGACACTG GGCCACAGGG CTTCCCAGGG ACCCCAGGAG ACGTGGGCCC CAAGGGCGAG 
AAGGGGGATC CTGGAGTCGG ACCGAGGGGA CCCCCAGGAC CCCAGGGGCC TCCGGGGCCC 
CCAGGGCCCT CCTTCAAACC CGACAGGCTG ACCTTCATCG ACATGGAGGG ATCCGGTTTT 
GGCGGCGACC TGGAGAGCCT CCGCGGCCCT CGCGGCTTCC CCGGCCCCCC AGGGCCCCCT 
GGCGTCCCAG GCCTGCCAGG AGAGCCAGGC CGCTTCGGGA TGAACAGCTC GGACGTCCCT 
GGACCCGCAG GCCTGCCCGG CGTGCCTGGG AGGGACGGGC CGCCTGGGCG TCCAGGGCCC 
CCGGGACCCC CAGGTACTCC AGGAAAAGAC GGACAACCAG GACTGACTGG ACAGAAAGGC 
AGCCTCGGTG AGGCAGGCGC CCCAGGACCC AAGGGAAGCA AGGGAGACCT TGGCCCCGTC 
GGCGCTCCTG GGGAGCATGG CCTGGCAGGA GCCCCTGGAC CCGCAGGCCC ACCAGGGCCC 
CCTGGCCCCC CGGGGCCCCC GGGCCCAGGA TTCGCCGCAG GGTTTGACGA CATGGAAGGC 
TCCGGGAGCC CCTTATGGTC AACAGCCCGA GGTGCCGAGG GGCTGCAGGG ACCGCCTGGC 
ACGCCCGGAG TCAAGGGGGA TCCTGGAGCT GTGGGGCCAC CAGGGAGCAA GGGAGAAGTT 
GGAGCAGACG GAGCACCGGG CCTCCCCGGC CTCCCGGGCA GAGAGGGTGC AGCTGGACCC 
CAGGGACCAA AAGGAGAAAA AGGCACTCAG GGAGAGAAAG GAGACCCAGG GAAGGATGGA 
GTGGGGCAGC CAGGACTCCC GGGACCCCCC GGACCCCCAG GGCCTGTCGT CTACGTGTCG 
GAGCAGGATA GAGTGCTGGC GAGCGTGCCA GGCCCCGAGG GCCGTGCAGG CTTCGCTGGC 
TTCCCCGGAC CAGCCGGGCC AAAGGGAGAC CTGGGCGCCA AAGGCCAGCA GGGCATCCCG 
GGACCCAAGG GTGAGAAGGG AGAGCCGGGC ACATTCTTCG GCCCCGATGG CAGAGCACTG 
ACCCCCGCCC AGAAAGGAGC CAAGGGTGAG CCTGGCTTCC GAGGACCTCC GGGTCCGTAT 
GGGCGGCCCG GGCACAAGGG AGAGATCGGA TTCCCTGGAC GGCCGGGGCG TCCCGGGATG 
AACGGGCTGA AAGGGGAGAA GGGGGAGCCG GGGGACGCCA GCGTGGGATT CGGCATGAGG 
GGTCCAGCTG GCCCACCTGG GCCTCCGGGG CCCCCAGGCC CTCCCGGGAC TCCTGTCTAC 
GACAGCAACG CATTTGTGGA CTCTGGCCGC CCTGGACCCC CAGGATTGCC AGGGCACCAG 
GGGCCTTCTG GACTCAAGGG TGACAAAGGA GACGTGGGCC CGCCCGGACC GCCAGGGCAG 
TTCCCGTTTG ACCTCCTCCA GCTGGGTGCC GAGATGAAGG GGGAGAAGGG GGACCGAGGG 
GCCGCTGGGC AGAAAGGAGA AAGGGGTGAG CCCGGGGGCG GAGGATTCTT CGGCTCTGGT 
GTGCCTGGCC CCCCCGGCCC GCCTGGCTAC CCAGGGATTC CGGGCCCCAA GGGAGAGAGC 
ATCCGCGGCC AGCCCGGCCC TCCGGGACCT CAGGGACCCC CTGGCATTGG CTACGAGGGA 
CCCCAGGGGC CTCCTGGGCC CCCCGGACCC CCAGGGCCCC CGGGACCCCC TTCCTTTCCT 
GGCCCCTACA GGCAGACCAT CAGTGTTCCT GGCCCGCCAG GCCCACCTGG GCCCCCAGGA 
CCCCCTGGAT CCATGGGCAC TTCTTCAGGG GTGAGGGTCT GGGCCACGTA CCAGATGCTG 
GTGGACCAGG TGCCGCAGGT GCCGGAGGGC TGGCTCATCT ACGTGGCTGA CAGGGAGGAG 
CTGTACGTCC GGGTCCGGAA TGGGTTCCGG AAGGTTCTGC TGGAGGCCCA CGTGCCCCTC 
CCCCATGGGA CGGACAACGA GGTGGCCGTC TTGCAGCCCC CGCTGGTGCA GCTGCATGAG 
GGTAATCCCT ACCCCCGCCG GGAGGTTCCA CACCCGACAG CGCGGTCCTG GCGGGCGGAC 
GACATCCTGG CCAGTCCCCC GCGCCTGCCG CACCCCCAGC CCTACCCCGG CGCCCCGCAC 
CACGGCGCCT ACGCACACCT CCGGCCGGTG CCCCCCACGG CCTCGCCCTC CCACACCCAC 
CACGACTTCC AGCCGGTGCT GCACCTGGTG GCGCTCAACA GCCCGCAGTC GGGCGGCCTG 
CGCGGCATCC GCGGCGCCGA CTTTCAGTGC TTCCAGCAGG CGCGCGCCGC GGGGCTGGCC 
GGCACCTTCC GCGCGTTCCT GTCCTCGCGG TTGCAGGACC TGTACAGCAT CGTGCGCCGC 
GCCGACCGTG CCACCCTGCC CGTCGTCAAC CTCAGGGACG AGGTGCTGTT TCCTAGCTGG 
GAGGCCTTGT TCTCAGGCTC CGAGGGCCAG CTGAAGCCCG GCGCCCGCAT CTTCTCCTTC 
GACGGCAGAG ATGTCCTTCA GCATCCCACC TGGCCCCAGA AGAGCGTGTG GCACGGCTCA 
GACCCCAGCG GGCGCCGGCT GACCGAGAGC TACTGCGAGA CGTGGCGGAC GGACAGCAGG 
GCGGCCACGG GCCAGGCCTC CTCGCTGCTG GCGGGCCGGC TGCTGGAGCA GAAAGCCGCC 
GGGTGCCACA ACGCCTTCAT TGTCCTCTGC ATCGAGAACA GCTTCATGAC CTCCTCCTCC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830844.1
CDS	37..5322
Translation	MAPDPSSGRGHHGLLLLACCLSAAQADLFSWIWSGNSAWPIAAPASKPPDSSPVWPTEDTTTHVAPRDAPTDWWKVSASPTAPLGRPEVGQGQTPTGPTASTVSLDPKEENIAGVGAKILNVAQGIRSFVQLWDNAKDTTPAESSAGAETPPPTDTMDDLSTPGPSITPQDSGTTLQLSTTALSSPDTQRTEAGTLPMPTQPPPSPGRPWFRESLVSPTPGRSSLSSVPAPAPPRGSQLPLGRPLQLDGEGLLLAAARPGQQHADDRRPMPPLPPLVTGSLGKHTALWALSSDPPAKLSHVAVSALTVDSGAWAPHVANSAGPGLANKSTLLGAAELAATEQPVPTTAGRCLPLLPSLALCGHLGIRRSWWPNHLHHTSREEVQAAVPAWGALLRTHCHRFLAWFFCLLLAPRCGPGLPPGLPPCRQFCEALEDACWSHLDGRGLPLPCASLPAREDGHCVFIGPPADEEVVEVGLQQLLGEPPPWQVAMVHDPDVGSAYEFGEDGSGGGQAARRLLPGTFFQDFSLLVRVRPASARAGVLFAVTDPAQAVVSVGVKLTAARGGRQHMQLLYTEPGATHTRTAASFELPALDGRWTRLALSVDGAYARLFVDCKEVQGQPLAHSLHDLQLEPDARLFVAQAGGADPEKFQGLISELRLRRDPQVSPRQCQDEDEDDDDDGASGDLGSGLVETQEHLREELGAPLGPRLPEAPPVTSPPLAGVGNEEDLRTEEVEESTTASSLGAQTLPSLGTVGTWDESVWSPGSSVKERGLKGQKGEPGAQGPPGPIGPQGPAGPAIDSHEAQPVSGPQGPPGPPGPPGKDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGEKGDPGVGPRGPPGPQGPPGPPGPSFKPDRLTFIDMEGSGFGGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGMNSSDVPGPAGLPGVPGRDGPPGRPGPPGPPGTPGKDGQPGLTGQKGSLGEAGAPGPKGSKGDLGPVGAPGEHGLAGAPGPAGPPGPPGPPGPPGPGFAAGFDDMEGSGSPLWSTARGAEGLQGPPGTPGVKGDPGAVGPPGSKGEVGADGAPGLPGLPGREGAAGPQGPKGEKGTQGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDRVLASVPGPEGRAGFAGFPGPAGPKGDLGAKGQQGIPGPKGEKGEPGTFFGPDGRALTPAQKGAKGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGMNGLKGEKGEPGDASVGFGMRGPAGPPGPPGPPGPPGTPVYDSNAFVDSGRPGPPGLPGHQGPSGLKGDKGDVGPPGPPGQFPFDLLQLGAEMKGEKGDRGAAGQKGERGEPGGGGFFGSGVPGPPGPPGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGPQGPPGPPGPPGPPGPPSFPGPYRQTISVPGPPGPPGPPGPPGSMGTSSGVRVWATYQMLVDQVPQVPEGWLIYVADREELYVRVRNGFRKVLLEAHVPLPHGTDNEVAVLQPPLVQLHEGNPYPRREVPHPTARSWRADDILASPPRLPHPQPYPGAPHHGAYAHLRPVPPTASPSHTHHDFQPVLHLVALNSPQSGGLRGIRGADFQCFQQARAAGLAGTFRAFLSSRLQDLYSIVRRADRATLPVVNLRDEVLFPSWEALFSGSEGQLKPGARIFSFDGRDVLQHPTWPQKSVWHGSDPSGRRLTESYCETWRTDSRAATGQASSLLAGRLLEQKAAGCHNAFIVLCIENSFMTSSSK

Target ORF information:

RefSeq Version	XM_024975076.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975076.1

ORF Insert Sequence:

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GGTAGATCTT CTCTTTCCTC CGTGCCGGCC CCGGCGCCTC CCCGGGGAAG CCAGCTGCCT 
CTCGGGCGGC CCCTGCAGCT GGACGGTGAA GGACTCTTGC TTGCAGCAGC CCGTCCCGGC 
CAGCAGCATG CTGACGACCG CAGGCCCATG CCGCCTCTGC CTCCCCTGGT GACGGGTTCC 
CTGGGCAAGC ACACTGCCCT CTGGGCTCTC TCCTCTGACC CCCCTGCCAA ACTGTCTCAT 
GTCGCAGTCT CAGCTCTAAC TGTGGACAGT GGTGCTTGGG CTCCCCACGT GGCTAATTCT 
GCGGGGCCTG GTCTTGCTAA CAAGTCCACA CTGCTCGGGG CTGCTGAGCT GGCTGCCACC 
GAGCAGCCTG TCCCCACCAC CGCTGGCCGC TGCCTGCCCC TGCTGCCCTC CCTGGCTCTC 
TGCGGCCACC TGGGCATCAG ACGCTCCTGG TGGCCCAACC ACCTCCACCA CACAAGTCGG 
GAGGAGGTGC AGGCCGCGGT CCCAGCCTGG GGGGCACTGC TCCGGACCCA CTGCCACCGC 
TTCCTGGCCT GGTTCTTCTG CCTGCTGCTG GCACCCCGCT GCGGGCCCGG CCTGCCGCCC 
GGCCTGCCAC CCTGTCGCCA GTTCTGCGAG GCCCTGGAGG ACGCATGCTG GAGCCACCTG 
GACGGGCGCG GGCTGCCACT CCCCTGCGCC TCGCTCCCGG CCCGCGAGGA CGGGCACTGC 
GTGTTCATAG GGCCACCGGC AGACGAAGAG GTCGTGGAGG TAGGGCTCCA GCAGCTGCTC 
GGGGAGCCCC CGCCCTGGCA GGTGGCCATG GTGCACGACC CCGACGTGGG GTCCGCCTAT 
GAGTTCGGCG AGGACGGCAG CGGTGGAGGC CAGGCGGCCC GGAGACTCCT GCCTGGCACG 
TTCTTCCAGG ACTTCTCGCT GCTTGTGCGT GTGCGGCCGG CCTCGGCGCG TGCGGGCGTG 
CTCTTTGCGG TCACAGACCC GGCGCAGGCA GTGGTCTCGG TGGGCGTGAA GCTGACGGCG 
GCGCGTGGGG GGCGGCAGCA CATGCAGCTG CTGTACACGG AGCCGGGAGC CACCCACACA 
CGCACAGCCG CCAGCTTCGA GCTGCCCGCG CTGGATGGTC GCTGGACGCG CCTGGCACTC 
AGCGTGGATG GCGCCTATGC CAGGCTCTTC GTGGACTGTA AGGAGGTCCA GGGCCAGCCC 
CTGGCGCACT CGCTGCACGA CCTGCAGCTG GAGCCGGACG CCCGCCTCTT CGTGGCCCAG 
GCCGGTGGAG CAGACCCTGA AAAGTTCCAG GGTCTGATCT CAGAGCTGAG GTTGCGCAGG 
GATCCTCAGG TGAGCCCCCG GCAGTGCCAG GATGAGGACG AGGACGACGA TGATGATGGG 
GCGTCCGGAG ACCTGGGCAG CGGGCTCGTG GAGACCCAGG AGCATCTCCG GGAGGAGTTG 
GGTGCGCCCC TGGGGCCCAG GCTCCCTGAG GCTCCTCCGG TCACTTCTCC ACCCTTGGCC 
GGAGTCGGCA ACGAGGAGGA CTTGAGAACT GAGGAAGTAG AGGAGTCAAC CACAGCATCT 
TCATTAGGAG CTCAGACCCT CCCCAGCTTG GGCACAGTTG GCACGTGGGA TGAGAGTGTT 
TGGAGCCCTG GGAGCAGCGT GAAAGAGCGA GGCCTGAAGG GGCAGAAGGG AGAGCCTGGC 
GCTCAGGGCC CCCCTGGCCC CATCGGCCCC CAGGGTCCTG CAGGCCCAGC CATAGACAGC 
CATGAGGCAC AGCCTGTTTC TGGACCACAG GGACCCCCTG GGCCCCCGGG ACCCCCGGGG 
AAGGACGGCA CCCCTGGAAG GGACGGTGAG CCTGGTGATC CTGGTGAAGA CGGGAAGCCC 
GGGGACACTG GGCCACAGGG CTTCCCAGGG ACCCCAGGAG ACGTGGGCCC CAAGGGCGAG 
AAGGGGGATC CTGGAGTCGG ACCGAGGGGA CCCCCAGGAC CCCAGGGGCC TCCGGGGCCC 
CCAGGGCCCT CCTTCAAACC CGACAGGCTG ACCTTCATCG ACATGGAGGG ATCCGGTTTT 
GGCGGCGACC TGGAGAGCCT CCGCGGCCCT CGCGGCTTCC CCGGCCCCCC AGGGCCCCCT 
GGCGTCCCAG GCCTGCCAGG AGAGCCAGGC CGCTTCGGGA TGAACAGCTC GGACGTCCCT 
GGACCCGCAG GCCTGCCCGG CGTGCCTGGG AGGGACGGGC CGCCTGGGCG TCCAGGGCCC 
CCGGGACCCC CAGGTACTCC AGGAAAAGAC GGACAACCAG GACTGACTGG ACAGAAAGGC 
AGCCTCGGTG AGGCAGGCGC CCCAGGACCC AAGGGAAGCA AGGGAGACCT TGGCCCCGTC 
GGCGCTCCTG GGGAGCATGG CCTGGCAGGA GCCCCTGGAC CCGCAGGCCC ACCAGGGCCC 
CCTGGCCCCC CGGGGCCCCC GGGCCCAGGA TTCGCCGCAG GGTTTGACGA CATGGAAGGC 
TCCGGGAGCC CCTTATGGTC AACAGCCCGA GGTGCCGAGG GGCTGCAGGG ACCGCCTGGC 
ACGCCCGGAG TCAAGGGGGA TCCTGGAGCT GTGGGGCCAC CAGGGAGCAA GGGAGAAGTT 
GGAGCAGACG GAGCACCGGG CCTCCCCGGC CTCCCGGGCA GAGAGGGTGC AGCTGGACCC 
CAGGGACCAA AAGGAGAAAA AGGCACTCAG GGAGAGAAAG GAGACCCAGG GAAGGATGGA 
GTGGGGCAGC CAGGACTCCC GGGACCCCCC GGACCCCCAG GGCCTGTCGT CTACGTGTCG 
GAGCAGGATA GAGTGCTGGC GAGCGTGCCA GGCCCCGAGG GCCGTGCAGG CTTCGCTGGC 
TTCCCCGGAC CAGCCGGGCC AAAGGGAGAC CTGGGCGCCA AAGGCCAGCA GGGCATCCCG 
GGACCCAAGG GTGAGAAGGG AGAGCCGGGC ACATTCTTCG GCCCCGATGG CAGAGCACTG 
ACCCCCGCCC AGAAAGGAGC CAAGGGTGAG CCTGGCTTCC GAGGACCTCC GGGTCCGTAT 
GGGCGGCCCG GGCACAAGGG AGAGATCGGA TTCCCTGGAC GGCCGGGGCG TCCCGGGATG 
AACGGGCTGA AAGGGGAGAA GGGGGAGCCG GGGGACGCCA GCGTGGGATT CGGCATGAGG 
GGTCCAGCTG GCCCACCTGG GCCTCCGGGG CCCCCAGGCC CTCCCGGGAC TCCTGTCTAC 
GACAGCAACG CATTTGTGGA CTCTGGCCGC CCTGGACCCC CAGGATTGCC AGGGCACCAG 
GGGCCTTCTG GACTCAAGGG TGACAAAGGA GACGTGGGCC CGCCCGGACC GCCAGGGCAG 
TTCCCGTTTG ACCTCCTCCA GCTGGGTGCC GAGATGAAGG GGGAGAAGGG GGACCGAGGG 
GCCGCTGGGC AGAAAGGAGA AAGGGGTGAG CCCGGGGGCG GAGGATTCTT CGGCTCTGGT 
GTGCCTGGCC CCCCCGGCCC GCCTGGCTAC CCAGGGATTC CGGGCCCCAA GGGAGAGAGC 
ATCCGCGGCC AGCCCGGCCC TCCGGGACCT CAGGGACCCC CTGGCATTGG CTACGAGGGA 
CCCCAGGGGC CTCCTGGGCC CCCCGGACCC CCAGGGCCCC CGGGACCCCC TTCCTTTCCT 
GGCCCCTACA GGCAGACCAT CAGTGTTCCT GGCCCGCCAG GCCCACCTGG GCCCCCAGGA 
CCCCCTGGAT CCATGGGCAC TTCTTCAGGG GTGAGGGTCT GGGCCACGTA CCAGATGCTG 
GTGGACCAGG TGCCGCAGGT GCCGGAGGGC TGGCTCATCT ACGTGGCTGA CAGGGAGGAG 
CTGTACGTCC GGGTCCGGAA TGGGTTCCGG AAGGTTCTGC TGGAGGCCCA CGTGCCCCTC 
CCCCATGGGA CGGACAACGA GGTGGCCGTC TTGCAGCCCC CGCTGGTGCA GCTGCATGAG 
GGTAATCCCT ACCCCCGCCG GGAGGTTCCA CACCCGACAG CGCGGTCCTG GCGGGCGGAC 
GACATCCTGG CCAGTCCCCC GCGCCTGCCG CACCCCCAGC CCTACCCCGG CGCCCCGCAC 
CACGGCGCCT ACGCACACCT CCGGCCGGTG CCCCCCACGG CCTCGCCCTC CCACACCCAC 
CACGACTTCC AGCCGGTGCT GCACCTGGTG GCGCTCAACA GCCCGCAGTC GGGCGGCCTG 
CGCGGCATCC GCGGCGCCGA CTTTCAGTGC TTCCAGCAGG CGCGCGCCGC GGGGCTGGCC 
GGCACCTTCC GCGCGTTCCT GTCCTCGCGG TTGCAGGACC TGTACAGCAT CGTGCGCCGC 
GCCGACCGTG CCACCCTGCC CGTCGTCAAC CTCAGGGACG AGGTGCTGTT TCCTAGCTGG 
GAGGCCTTGT TCTCAGGCTC CGAGGGCCAG CTGAAGCCCG GCGCCCGCAT CTTCTCCTTC 
GACGGCAGAG ATGTCCTTCA GCATCCCACC TGGCCCCAGA AGAGCGTGTG GCACGGCTCA 
GACCCCAGCG GGCGCCGGCT GACCGAGAGC TACTGCGAGA CGTGGCGGAC GGACAGCAGG 
GCGGCCACGG GCCAGGCCTC CTCGCTGCTG GCGGGCCGGC TGCTGGAGCA GAAAGCCGCC 
GGGTGCCACA ACGCCTTCAT TGTCCTCTGC ATCGAGAACA GCTTCATGAC CTCCTCCTCC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( XM_024975114.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830882.1 Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X4, mRNA.

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CloneID	OBa218674
Clone ID Related Accession (Same CDS sequence)	XM_024975114.1
Accession Version	XM_024975114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVIII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GACGAAGAGG TCGTGGAGGT AGGGCTCCAG CAGCTGCTCG GGGAGCCCCC GCCCTGGCAG 
GTGGCCATGG TGCACGACCC CGACGTGGGG TCCGCCTATG AGTTCGGCGA GGACGGCAGC 
GGTGGAGGCC AGGCGGCCCG GAGACTCCTG CCTGGCACGT TCTTCCAGGA CTTCTCGCTG 
CTTGTGCGTG TGCGGCCGGC CTCGGCGCGT GCGGGCGTGC TCTTTGCGGT CACAGACCCG 
GCGCAGGCAG TGGTCTCGGT GGGCGTGAAG CTGACGGCGG CGCGTGGGGG GCGGCAGCAC 
ATGCAGCTGC TGTACACGGA GCCGGGAGCC ACCCACACAC GCACAGCCGC CAGCTTCGAG 
CTGCCCGCGC TGGATGGTCG CTGGACGCGC CTGGCACTCA GCGTGGATGG CGCCTATGCC 
AGGCTCTTCG TGGACTGTAA GGAGGTCCAG GGCCAGCCCC TGGCGCACTC GCTGCACGAC 
CTGCAGCTGG AGCCGGACGC CCGCCTCTTC GTGGCCCAGG CCGGTGGAGC AGACCCTGAA 
AAGTTCCAGG GTCTGATCTC AGAGCTGAGG TTGCGCAGGG ATCCTCAGGT GAGCCCCCGG 
CAGTGCCAGG ATGAGGACGA GGACGACGAT GATGATGGGG CGTCCGGAGA CCTGGGCAGC 
GGGCTCGTGG AGACCCAGGA GCATCTCCGG GAGGAGTTGG GTGCGCCCCT GGGGCCCAGG 
CTCCCTGAGG CTCCTCCGGT CACTTCTCCA CCCTTGGCCG GAGTCGGCAA CGAGGAGGAC 
TTGAGAACTG AGGAAGTAGA GGAGTCAACC ACAGCATCTT CATTAGGAGC TCAGACCCTC 
CCCAGCTTGG GCACAGTTGG CACGTGGGAT GAGAGTGTTT GGAGCCCTGG GAGCAGCGTG 
AAAGAGCGAG GCCTGAAGGG GCAGAAGGGA GAGCCTGGCG CTCAGGGCCC CCCTGGCCCC 
ATCGGCCCCC AGGGTCCTGC AGGCCCAGCC ATAGACAGCC ATGAGGCACA GCCTGTTTCT 
GGACCACAGG GACCCCCTGG GCCCCCGGGA CCCCCGGGGA AGGACGGCAC CCCTGGAAGG 
GACGGTGAGC CTGGTGATCC TGGTGAAGAC GGGAAGCCCG GGGACACTGG GCCACAGGGC 
TTCCCAGGGA CCCCAGGAGA CGTGGGCCCC AAGGGCGAGA AGGGGGATCC TGGAGTCGGA 
CCGAGGGGAC CCCCAGGACC CCAGGGGCCT CCGGGGCCCC CAGGGCCCTC CTTCAAACCC 
GACAGGCTGA CCTTCATCGA CATGGAGGGA TCCGGTTTTG GCGGCGACCT GGAGAGCCTC 
CGCGGCCCTC GCGGCTTCCC CGGCCCCCCA GGGCCCCCTG GCGTCCCAGG CCTGCCAGGA 
GAGCCAGGCC GCTTCGGGAT GAACAGCTCG GACGTCCCTG GACCCGCAGG CCTGCCCGGC 
GTGCCTGGGA GGGACGGGCC GCCTGGGCGT CCAGGGCCCC CGGGACCCCC AGGTACTCCA 
GGAAAAGACG GACAACCAGG ACTGACTGGA CAGAAAGGCA GCCTCGGTGA GGCAGGCGCC 
CCAGGACCCA AGGGAAGCAA GGGAGACCTT GGCCCCGTCG GCGCTCCTGG GGAGCATGGC 
CTGGCAGGAG CCCCTGGACC CGCAGGCCCA CCAGGGCCCC CTGGCCCCCC GGGGCCCCCG 
GGCCCAGGAT TCGCCGCAGG GTTTGACGAC ATGGAAGGCT CCGGGAGCCC CTTATGGTCA 
ACAGCCCGAG GTGCCGAGGG GCTGCAGGGA CCGCCTGGCA CGCCCGGAGT CAAGGGGGAT 
CCTGGAGCTG TGGGGCCACC AGGGAGCAAG GGAGAAGTTG GAGCAGACGG AGCACCGGGC 
CTCCCCGGCC TCCCGGGCAG AGAGGGTGCA GCTGGACCCC AGGGACCAAA AGGAGAAAAA 
GGCACTCAGG GAGAGAAAGG AGACCCAGGG AAGGATGGAG TGGGGCAGCC AGGACTCCCG 
GGACCCCCCG GACCCCCAGG GCCTGTCGTC TACGTGTCGG AGCAGGATAG AGTGCTGGCG 
AGCGTGCCAG GCCCCGAGGG CCGTGCAGGC TTCGCTGGCT TCCCCGGACC AGCCGGGCCA 
AAGGGAGACC TGGGCGCCAA AGGCCAGCAG GGCATCCCGG GACCCAAGGG TGAGAAGGGA 
GAGCCGGGCA CATTCTTCGG CCCCGATGGC AGAGCACTGA CCCCCGCCCA GAAAGGAGCC 
AAGGGTGAGC CTGGCTTCCG AGGACCTCCG GGTCCGTATG GGCGGCCCGG GCACAAGGGA 
GAGATCGGAT TCCCTGGACG GCCGGGGCGT CCCGGGATGA ACGGGCTGAA AGGGGAGAAG 
GGGGAGCCGG GGGACGCCAG CGTGGGATTC GGCATGAGGG GTCCAGCTGG CCCACCTGGG 
CCTCCGGGGC CCCCAGGCCC TCCCGGGACT CCTGTCTACG ACAGCAACGC ATTTGTGGAC 
TCTGGCCGCC CTGGACCCCC AGGATTGCCA GGGCACCAGG GGCCTTCTGG ACTCAAGGGT 
GACAAAGGAG ACGTGGGCCC GCCCGGACCG CCAGGGCAGT TCCCGTTTGA CCTCCTCCAG 
CTGGGTGCCG AGATGAAGGG GGAGAAGGGG GACCGAGGGG CCGCTGGGCA GAAAGGAGAA 
AGGGGTGAGC CCGGGGGCGG AGGATTCTTC GGCTCTGGTG TGCCTGGCCC CCCCGGCCCG 
CCTGGCTACC CAGGGATTCC GGGCCCCAAG GGAGAGAGCA TCCGCGGCCA GCCCGGCCCT 
CCGGGACCTC AGGGACCCCC TGGCATTGGC TACGAGGGAC CCCAGGGGCC TCCTGGGCCC 
CCCGGACCCC CAGGGCCCCC GGGACCCCCT TCCTTTCCTG GCCCCTACAG GCAGACCATC 
AGTGTTCCTG GCCCGCCAGG CCCACCTGGG CCCCCAGGAC CCCCTGGATC CATGGGCACT 
TCTTCAGGGG GATCCACTGG GCTGGGCTTC CAGCAGGTGA GGGTCTGGGC CACGTACCAG 
ATGCTGGTGG ACCAGGTGCC GCAGGTGCCG GAGGGCTGGC TCATCTACGT GGCTGACAGG 
GAGGAGCTGT ACGTCCGGGT CCGGAATGGG TTCCGGAAGG TTCTGCTGGA GGCCCACGTG 
CCCCTCCCCC ATGGGACGGA CAACGAGGTG GCCGTCTTGC AGCCCCCGCT GGTGCAGCTG 
CATGAGGGTA ATCCCTACCC CCGCCGGGAG GTTCCACACC CGACAGCGCG GTCCTGGCGG 
GCGGACGACA TCCTGGCCAG TCCCCCGCGC CTGCCGCACC CCCAGCCCTA CCCCGGCGCC 
CCGCACCACG GCGCCTACGC ACACCTCCGG CCGGTGCCCC CCACGGCCTC GCCCTCCCAC 
ACCCACCACG ACTTCCAGCC GGTGCTGCAC CTGGTGGCGC TCAACAGCCC GCAGTCGGGC 
GGCCTGCGCG GCATCCGCGG CGCCGACTTT CAGTGCTTCC AGCAGGCGCG CGCCGCGGGG 
CTGGCCGGCA CCTTCCGCGC GTTCCTGTCC TCGCGGTTGC AGGACCTGTA CAGCATCGTG 
CGCCGCGCCG ACCGTGCCAC CCTGCCCGTC GTCAACCTCA GGGACGAGGT GCTGTTTCCT 
AGCTGGGAGG CCTTGTTCTC AGGCTCCGAG GGCCAGCTGA AGCCCGGCGC CCGCATCTTC 
TCCTTCGACG GCAGAGATGT CCTTCAGCAT CCCACCTGGC CCCAGAAGAG CGTGTGGCAC 
GGCTCAGACC CCAGCGGGCG CCGGCTGACC GAGAGCTACT GCGAGACGTG GCGGACGGAC 
AGCAGGGCGG CCACGGGCCA GGCCTCCTCG CTGCTGGCGG GCCGGCTGCT GGAGCAGAAA 
GCCGCCGGGT GCCACAACGC CTTCATTGTC CTCTGCATCG AGAACAGCTT CATGACCTCC 
TCCTCCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830882.1
CDS	45..4616
Translation	MAPDPSSGRGHHGLLLLACCLSAAQADLFSWIWSGNSAWPIAAPASKPPDSSPVWPTEDTTTHVAPRDAPTDWWKVSASPTAPLGRPEVGQGQTPTGPTASTVSLDPKEENIAGVGAKILNVAQGIRSFVQLWDNAKDTTPAESSAGAETPPPTDTMDDLSTPGPSITPQDSGTTLQLSTTALSSPDTQRTEAGTLPMPTQPPPSPGRPWFRESLVSPTPDEEVVEVGLQQLLGEPPPWQVAMVHDPDVGSAYEFGEDGSGGGQAARRLLPGTFFQDFSLLVRVRPASARAGVLFAVTDPAQAVVSVGVKLTAARGGRQHMQLLYTEPGATHTRTAASFELPALDGRWTRLALSVDGAYARLFVDCKEVQGQPLAHSLHDLQLEPDARLFVAQAGGADPEKFQGLISELRLRRDPQVSPRQCQDEDEDDDDDGASGDLGSGLVETQEHLREELGAPLGPRLPEAPPVTSPPLAGVGNEEDLRTEEVEESTTASSLGAQTLPSLGTVGTWDESVWSPGSSVKERGLKGQKGEPGAQGPPGPIGPQGPAGPAIDSHEAQPVSGPQGPPGPPGPPGKDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGEKGDPGVGPRGPPGPQGPPGPPGPSFKPDRLTFIDMEGSGFGGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGMNSSDVPGPAGLPGVPGRDGPPGRPGPPGPPGTPGKDGQPGLTGQKGSLGEAGAPGPKGSKGDLGPVGAPGEHGLAGAPGPAGPPGPPGPPGPPGPGFAAGFDDMEGSGSPLWSTARGAEGLQGPPGTPGVKGDPGAVGPPGSKGEVGADGAPGLPGLPGREGAAGPQGPKGEKGTQGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDRVLASVPGPEGRAGFAGFPGPAGPKGDLGAKGQQGIPGPKGEKGEPGTFFGPDGRALTPAQKGAKGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGMNGLKGEKGEPGDASVGFGMRGPAGPPGPPGPPGPPGTPVYDSNAFVDSGRPGPPGLPGHQGPSGLKGDKGDVGPPGPPGQFPFDLLQLGAEMKGEKGDRGAAGQKGERGEPGGGGFFGSGVPGPPGPPGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGPQGPPGPPGPPGPPGPPSFPGPYRQTISVPGPPGPPGPPGPPGSMGTSSGGSTGLGFQQVRVWATYQMLVDQVPQVPEGWLIYVADREELYVRVRNGFRKVLLEAHVPLPHGTDNEVAVLQPPLVQLHEGNPYPRREVPHPTARSWRADDILASPPRLPHPQPYPGAPHHGAYAHLRPVPPTASPSHTHHDFQPVLHLVALNSPQSGGLRGIRGADFQCFQQARAAGLAGTFRAFLSSRLQDLYSIVRRADRATLPVVNLRDEVLFPSWEALFSGSEGQLKPGARIFSFDGRDVLQHPTWPQKSVWHGSDPSGRRLTESYCETWRTDSRAATGQASSLLAGRLLEQKAAGCHNAFIVLCIENSFMTSSSK

Target ORF information:

RefSeq Version	XM_024975114.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975114.1

ORF Insert Sequence:

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GACGAAGAGG TCGTGGAGGT AGGGCTCCAG CAGCTGCTCG GGGAGCCCCC GCCCTGGCAG 
GTGGCCATGG TGCACGACCC CGACGTGGGG TCCGCCTATG AGTTCGGCGA GGACGGCAGC 
GGTGGAGGCC AGGCGGCCCG GAGACTCCTG CCTGGCACGT TCTTCCAGGA CTTCTCGCTG 
CTTGTGCGTG TGCGGCCGGC CTCGGCGCGT GCGGGCGTGC TCTTTGCGGT CACAGACCCG 
GCGCAGGCAG TGGTCTCGGT GGGCGTGAAG CTGACGGCGG CGCGTGGGGG GCGGCAGCAC 
ATGCAGCTGC TGTACACGGA GCCGGGAGCC ACCCACACAC GCACAGCCGC CAGCTTCGAG 
CTGCCCGCGC TGGATGGTCG CTGGACGCGC CTGGCACTCA GCGTGGATGG CGCCTATGCC 
AGGCTCTTCG TGGACTGTAA GGAGGTCCAG GGCCAGCCCC TGGCGCACTC GCTGCACGAC 
CTGCAGCTGG AGCCGGACGC CCGCCTCTTC GTGGCCCAGG CCGGTGGAGC AGACCCTGAA 
AAGTTCCAGG GTCTGATCTC AGAGCTGAGG TTGCGCAGGG ATCCTCAGGT GAGCCCCCGG 
CAGTGCCAGG ATGAGGACGA GGACGACGAT GATGATGGGG CGTCCGGAGA CCTGGGCAGC 
GGGCTCGTGG AGACCCAGGA GCATCTCCGG GAGGAGTTGG GTGCGCCCCT GGGGCCCAGG 
CTCCCTGAGG CTCCTCCGGT CACTTCTCCA CCCTTGGCCG GAGTCGGCAA CGAGGAGGAC 
TTGAGAACTG AGGAAGTAGA GGAGTCAACC ACAGCATCTT CATTAGGAGC TCAGACCCTC 
CCCAGCTTGG GCACAGTTGG CACGTGGGAT GAGAGTGTTT GGAGCCCTGG GAGCAGCGTG 
AAAGAGCGAG GCCTGAAGGG GCAGAAGGGA GAGCCTGGCG CTCAGGGCCC CCCTGGCCCC 
ATCGGCCCCC AGGGTCCTGC AGGCCCAGCC ATAGACAGCC ATGAGGCACA GCCTGTTTCT 
GGACCACAGG GACCCCCTGG GCCCCCGGGA CCCCCGGGGA AGGACGGCAC CCCTGGAAGG 
GACGGTGAGC CTGGTGATCC TGGTGAAGAC GGGAAGCCCG GGGACACTGG GCCACAGGGC 
TTCCCAGGGA CCCCAGGAGA CGTGGGCCCC AAGGGCGAGA AGGGGGATCC TGGAGTCGGA 
CCGAGGGGAC CCCCAGGACC CCAGGGGCCT CCGGGGCCCC CAGGGCCCTC CTTCAAACCC 
GACAGGCTGA CCTTCATCGA CATGGAGGGA TCCGGTTTTG GCGGCGACCT GGAGAGCCTC 
CGCGGCCCTC GCGGCTTCCC CGGCCCCCCA GGGCCCCCTG GCGTCCCAGG CCTGCCAGGA 
GAGCCAGGCC GCTTCGGGAT GAACAGCTCG GACGTCCCTG GACCCGCAGG CCTGCCCGGC 
GTGCCTGGGA GGGACGGGCC GCCTGGGCGT CCAGGGCCCC CGGGACCCCC AGGTACTCCA 
GGAAAAGACG GACAACCAGG ACTGACTGGA CAGAAAGGCA GCCTCGGTGA GGCAGGCGCC 
CCAGGACCCA AGGGAAGCAA GGGAGACCTT GGCCCCGTCG GCGCTCCTGG GGAGCATGGC 
CTGGCAGGAG CCCCTGGACC CGCAGGCCCA CCAGGGCCCC CTGGCCCCCC GGGGCCCCCG 
GGCCCAGGAT TCGCCGCAGG GTTTGACGAC ATGGAAGGCT CCGGGAGCCC CTTATGGTCA 
ACAGCCCGAG GTGCCGAGGG GCTGCAGGGA CCGCCTGGCA CGCCCGGAGT CAAGGGGGAT 
CCTGGAGCTG TGGGGCCACC AGGGAGCAAG GGAGAAGTTG GAGCAGACGG AGCACCGGGC 
CTCCCCGGCC TCCCGGGCAG AGAGGGTGCA GCTGGACCCC AGGGACCAAA AGGAGAAAAA 
GGCACTCAGG GAGAGAAAGG AGACCCAGGG AAGGATGGAG TGGGGCAGCC AGGACTCCCG 
GGACCCCCCG GACCCCCAGG GCCTGTCGTC TACGTGTCGG AGCAGGATAG AGTGCTGGCG 
AGCGTGCCAG GCCCCGAGGG CCGTGCAGGC TTCGCTGGCT TCCCCGGACC AGCCGGGCCA 
AAGGGAGACC TGGGCGCCAA AGGCCAGCAG GGCATCCCGG GACCCAAGGG TGAGAAGGGA 
GAGCCGGGCA CATTCTTCGG CCCCGATGGC AGAGCACTGA CCCCCGCCCA GAAAGGAGCC 
AAGGGTGAGC CTGGCTTCCG AGGACCTCCG GGTCCGTATG GGCGGCCCGG GCACAAGGGA 
GAGATCGGAT TCCCTGGACG GCCGGGGCGT CCCGGGATGA ACGGGCTGAA AGGGGAGAAG 
GGGGAGCCGG GGGACGCCAG CGTGGGATTC GGCATGAGGG GTCCAGCTGG CCCACCTGGG 
CCTCCGGGGC CCCCAGGCCC TCCCGGGACT CCTGTCTACG ACAGCAACGC ATTTGTGGAC 
TCTGGCCGCC CTGGACCCCC AGGATTGCCA GGGCACCAGG GGCCTTCTGG ACTCAAGGGT 
GACAAAGGAG ACGTGGGCCC GCCCGGACCG CCAGGGCAGT TCCCGTTTGA CCTCCTCCAG 
CTGGGTGCCG AGATGAAGGG GGAGAAGGGG GACCGAGGGG CCGCTGGGCA GAAAGGAGAA 
AGGGGTGAGC CCGGGGGCGG AGGATTCTTC GGCTCTGGTG TGCCTGGCCC CCCCGGCCCG 
CCTGGCTACC CAGGGATTCC GGGCCCCAAG GGAGAGAGCA TCCGCGGCCA GCCCGGCCCT 
CCGGGACCTC AGGGACCCCC TGGCATTGGC TACGAGGGAC CCCAGGGGCC TCCTGGGCCC 
CCCGGACCCC CAGGGCCCCC GGGACCCCCT TCCTTTCCTG GCCCCTACAG GCAGACCATC 
AGTGTTCCTG GCCCGCCAGG CCCACCTGGG CCCCCAGGAC CCCCTGGATC CATGGGCACT 
TCTTCAGGGG GATCCACTGG GCTGGGCTTC CAGCAGGTGA GGGTCTGGGC CACGTACCAG 
ATGCTGGTGG ACCAGGTGCC GCAGGTGCCG GAGGGCTGGC TCATCTACGT GGCTGACAGG 
GAGGAGCTGT ACGTCCGGGT CCGGAATGGG TTCCGGAAGG TTCTGCTGGA GGCCCACGTG 
CCCCTCCCCC ATGGGACGGA CAACGAGGTG GCCGTCTTGC AGCCCCCGCT GGTGCAGCTG 
CATGAGGGTA ATCCCTACCC CCGCCGGGAG GTTCCACACC CGACAGCGCG GTCCTGGCGG 
GCGGACGACA TCCTGGCCAG TCCCCCGCGC CTGCCGCACC CCCAGCCCTA CCCCGGCGCC 
CCGCACCACG GCGCCTACGC ACACCTCCGG CCGGTGCCCC CCACGGCCTC GCCCTCCCAC 
ACCCACCACG ACTTCCAGCC GGTGCTGCAC CTGGTGGCGC TCAACAGCCC GCAGTCGGGC 
GGCCTGCGCG GCATCCGCGG CGCCGACTTT CAGTGCTTCC AGCAGGCGCG CGCCGCGGGG 
CTGGCCGGCA CCTTCCGCGC GTTCCTGTCC TCGCGGTTGC AGGACCTGTA CAGCATCGTG 
CGCCGCGCCG ACCGTGCCAC CCTGCCCGTC GTCAACCTCA GGGACGAGGT GCTGTTTCCT 
AGCTGGGAGG CCTTGTTCTC AGGCTCCGAG GGCCAGCTGA AGCCCGGCGC CCGCATCTTC 
TCCTTCGACG GCAGAGATGT CCTTCAGCAT CCCACCTGGC CCCAGAAGAG CGTGTGGCAC 
GGCTCAGACC CCAGCGGGCG CCGGCTGACC GAGAGCTACT GCGAGACGTG GCGGACGGAC 
AGCAGGGCGG CCACGGGCCA GGCCTCCTCG CTGCTGGCGG GCCGGCTGCT GGAGCAGAAA 
GCCGCCGGGT GCCACAACGC CTTCATTGTC CTCTGCATCG AGAACAGCTT CATGACCTCC 
TCCTCCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL18A1 ( NM_001083388.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001076857.1 Bos taurus collagen type XVIII alpha 1 chain (COL18A1), mRNA.

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CloneID	OBa38439
Clone ID Related Accession (Same CDS sequence)	NM_001083388.2
Accession Version	NM_001083388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVIII) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123531.1. On Aug 31, 2012 this sequence version replaced NM_001083388.1. ##Evidence-Data-START## Transcript exon combination :: BC123531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GACGAAAAGG TCGTGGAGGT AGGGCTCCAG CAGCTGCTCG GGGAGCCCCC GCCCTGGCAG 
GTGGCCATGG TGCACGACCC CGACGTGGGG TCCGCCTATG AGTTCGGCGA GGACGGCAGC 
GGTGGAGGCC AGGCGGCCCG GAGACTCCTG CCTGGCACGT TCTTCCAGGA CTTCTCGCTG 
CTTGTGCGTG TGCGGCCGGC CTCGGCGCGT GCGGGCGTGC TCTTTGCGGT CACAGACCCG 
GCGCAGGCAG TGGTCTCGGT GGGCGTGAAG CTGACGGCGG CGCGTGGGGG GCGGCAGCAC 
ATGCAGCTGC TGTACACGGA GCCGGGAGCC ACCCACACAC GCACAGCCGC CAGCTTCGAG 
CTGCCCGCGC TGGATGGTCG CTGGACGCGC CTGGCACTCA GCGTGGATGG CGCCTATGCC 
AGGCTCTTCG TGGACTGTAA GGAGGTCCAG GGCCAGCCCC TGGCGCACTC GCTGCACGAC 
CTGCAGCTGG AGCCGGACGC CCGCCTCTTC GTGGCCCAGG CCGGTGGAGC AGACCCTGAA 
AAGTTCCAGG GTCTGATCTC AGAGCTGAGG TTGCGCAGGG ATCCTCAGGT GAGCCCCCGG 
CAGTGCCAGG ATGAGGACGA GGACGACGAT GATGATGGGG CGTCCGGAGA CCTGGGCAGC 
GGGCTCGTGG AGACCCAGGA GCATCTCCGG GAGGAGTTGG GTGCGCCCCT GGGGCCCAGG 
CTCCCTGAGG CTCCTCCGGT CACTTCTCCA CCCTTGGCCG GAGTCGGCAA CGAGGAGGAC 
TTGAGAACTG AGGAAGTAGA GGAGTCAACC ACAGCATCTT CATTAGGAGC TCAGACCCTC 
CCCAGCTTGG GCACAGTTGG CACGTGGGAT GAGAGTGTTT GGAGCCCTGG GAGCAGCGTG 
AAAGAGCGAG GCCTGAAGGG GCAGAAGGGA GAGCCTGGCG CTCAGGGCCC CCCTGGCCCC 
ATCGGCCCCC AGGGTCCTGC AGGCCCAGCC ATAGACAGCC ATGAGGCACA GCCTGTTTCT 
GGACCACAGG GACCCCCTGG GCCCCCGGGA CCCCCGGGGA AGGACGGCAC CCCTGGAAGG 
GACGGTGAGC CTGGTGATCC TGGTGAAGAC GGGAAGCCCG GGGACACTGG GCCACAGGGC 
TTCCCAGGGA CCCCAGGAGA CGTGGGCCCC AAGGGCGAGA AGGGGGATCC TGGAGTCGGA 
CCGAGGGGAC CCCCAGGACC CCAGGGGCCT CCGGGGCCCC CAGGGCCCTC CTTCAAACCC 
GACAGGCTGA CCTTCATCGA CATGGAGGGA TCCGGTTTTG GCGGCGACCT GGAGAGCCTC 
CGCGGCCCTC GCGGCTTCCC CGGCCCCCCA GGGCCCCCTG GCGTCCCAGG CCTGCCAGGA 
GAGCCAGGCC GCTTCGGGAT GAACAGCTCG GACGTCCCTG GACCCGCAGG CCTGCCCGGC 
GTGCCTGGGA GGGACGGGCC GCCTGGGCGT CCAGGGCCCC CGGGACCCCC AGGTACTCCA 
GGAAAAGACG GACAACCAGG ACTGACTGGA CAGAAAGGCA GCCTCGGTGA GGCAGGCGCC 
CCAGGACCCA AGGGAAGCAA GGGAGACCTT GGCCCCGTCG GCGCTCCTGG GGAGCATGGC 
CTGGCAGGAG CCCCTGGACC CGCAGGCCCA CCAGGGCCCC CTGGCCCCCC GGGGCCCCCG 
GGCCCAGGAT TCGCCGCAGG GTTTGACGAC ATGGAAGGCT CCGGGAGCCC CTTATGGTCA 
ACAGCCCGAG GTGCCGAGGG GCTGCAGGGA CCGCCTGGCA CGCCCGGAGT CAAGGGGGAT 
CCTGGAGCTG TGGGGCCACC AGGGAGCAAG GGAGAAGTTG GAGCAGACGG AGCACCGGGC 
CTCCCCGGCC TCCCGGGCAG AGAGGGTGCA GCTGGACCCC AGGGACCAAA AGGAGAAAAA 
GGCACTCAGG GAGAGAAAGG AGACCCAGGG AAGGATGGAG TGGGGCAGCC AGGACTCCCG 
GGACCCCCCG GACCCCCAGG GCCTGTCGTC TACGTGTCGG AGCAGGATAG AGTGCTGGCG 
AGCGTGCCAG GCCCCGAGGG CCGTGCAGGC TTCGCTGGCT TCCCCGGACC AGCCGGGCCA 
AAGGGAGACC TGGGCGCCAA AGGCCAGCAG GGCATCCCGG GACCCAAGGG TGAGAAGGGA 
GAGCCGGGCA CATTCTTCGG CCCCGATGGC AGAGCACTGA CCCCCGCCCA GAAAGGAGCC 
AAGGGTGAGC CTGGCTTCCG AGGACCTCCG GGTCCGTATG GGCGGCCCGG GCACAAGGGA 
GAGATCGGAT TCCCTGGACG GCCGGGGCGT CCCGGGATGA ACGGGCTGAA AGGGGAGAAG 
GGGGAGCCGG GGGACGCCAG CGTGGGATTC GGCATGAGGG GTCCAGCTGG CCCACCTGGG 
CCTCCGGGGC CCCCAGGCCC TCCCGGGACT CCTGTCTACG ACAGCAACGC ATTTGTGGAC 
TCTGGCCGCC CTGGACCCCC AGGATTGCCA GGGCACCAGG GGCCTTCTGG ACTCAAGGGT 
GACAAAGGAG ACGTGGGCCC GCCCGGACCG CCAGGGCAGT TCCCGTTTGA CCTCCTCCAG 
CTGGGTGCCG AGATGAAGGG GGAGAAGGGG GACCGAGGGG CCGCTGGGCA GAAAGGAGAA 
AGGGGTGAGC CCGGGGGCGG AGGATTCTTC GGCTCTGGTG TGCCTGGCCC CCCCGGCCCG 
CCTGGCTACC CAGGGATTCC GGGCCCCAAG GGAGAGAGCA TCCGCGGCCA GCCCGGCCCT 
CCGGGACCTC AGGGACCCCC TGGCATTGGC TACGAGGGAC CCCAGGGGCC TCCTGGGCCC 
CCCGGACCCC CAGGGCCCCC GGGACCCCCT TCCTTTCCTG GCCCCTACAG GCAGACCATC 
AGTGTTCCTG GCCCGCCAGG CCCACCTGGG CCCCCAGGAC CCCCTGGATC CATGGGCACT 
TCTTCAGGGG TGAGGGTCTG GGCCACGTAC CAGATGCTGG TGGACCAGGT GCCGCAGGTG 
CCGGAGGGCT GGCTCATCTA CGTGGCTGAC AGGGAGGAGC TGTACGTCCG GGTCCGGAAT 
GGGTTCCGGA AGGTTCTGCT GGAGGCCCAC GTGCCCCTCC CCCATGGGAC GGACAACGAG 
GTGGCCGTCT TGCAGCCCCC GCTGGTGCAG CTGCATGAGG GTAATCCCTA CCCCCGCCGG 
GAGGTTCCAC ACCCGACAGC GCGGTCCTGG CGGGCGGACG ACATCCTGGC CAGTCCCCCG 
CGCCTGCCGC ACCCCCAGCC CTACCCCGGC GCCCCGCACC ACGGCGCCTA CGCACACCTC 
CGGCCGGTGC CCCCCACGGC CTCGCCCTCC CACACCCACC ACGACTTCCA GCCGGTGCTG 
CACCTGGTGG CGCTCAACAG CCCGCAGTCG GGCGGCCTGC GCGGCATCCG CGGCGCCGAC 
TTTCAGTGCT TCCAGCAGGC GCGCGCCGCG GGGCTGGCCG GCACCTTCCG CGCGTTCCTG 
TCCTCGCGGT TGCAGGACCT GTACAGCATC GTGCGCCGCG CCGACCGTGC CACCCTGCCC 
GTCGTCAACC TCAGGGACGA GGTGCTGTTT CCTAGCTGGG AGGCCTTGTT CTCAGGCTCC 
GAGGGCCAGC TGAAGCCCGG CGCCCGCATC TTCTCCTTCG ACGGCAGAGA TGTCCTTCAG 
CATCCCACCT GGCCCCAGAA GAGCGTGTGG CACGGCTCAG ACCCCAGCGG GCGCCGGCTG 
ACCGAGAGCT ACTGCGAGAC GTGGCGGACG GACAGCAGGG CGGCCACGGG CCAGGCCTCC 
TCGCTGCTGG CGGGCCGGCT GCTGGAGCAG AAAGCCGCCG GGTGCCACAA CGCCTTCATT 
GTCCTCTGCA TCGAGAACAG CTTCATGACC TCCTCCTCCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076857.1
CDS	42..4586
Translation	MAPDPSSGRGHHGLLLLACCLSAAQADLFSWIWSGNSAWPIAAPASKPPDSSPVWPTEDTTTHVAPRDAPTDWWKVSASPTAPLGRPEVGQGQTPTGPTASTVSLDPKEENIAGVGAKILNVAQGIRSFVQLWDNAKDTTPAESSAGAETPPPTDTMDDLSTPGPSITPQDSGTTLQLSTTALSSPDTQRTEAGTLPMPTQPPPSPGRPWFRESLVSPTPDEKVVEVGLQQLLGEPPPWQVAMVHDPDVGSAYEFGEDGSGGGQAARRLLPGTFFQDFSLLVRVRPASARAGVLFAVTDPAQAVVSVGVKLTAARGGRQHMQLLYTEPGATHTRTAASFELPALDGRWTRLALSVDGAYARLFVDCKEVQGQPLAHSLHDLQLEPDARLFVAQAGGADPEKFQGLISELRLRRDPQVSPRQCQDEDEDDDDDGASGDLGSGLVETQEHLREELGAPLGPRLPEAPPVTSPPLAGVGNEEDLRTEEVEESTTASSLGAQTLPSLGTVGTWDESVWSPGSSVKERGLKGQKGEPGAQGPPGPIGPQGPAGPAIDSHEAQPVSGPQGPPGPPGPPGKDGTPGRDGEPGDPGEDGKPGDTGPQGFPGTPGDVGPKGEKGDPGVGPRGPPGPQGPPGPPGPSFKPDRLTFIDMEGSGFGGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGMNSSDVPGPAGLPGVPGRDGPPGRPGPPGPPGTPGKDGQPGLTGQKGSLGEAGAPGPKGSKGDLGPVGAPGEHGLAGAPGPAGPPGPPGPPGPPGPGFAAGFDDMEGSGSPLWSTARGAEGLQGPPGTPGVKGDPGAVGPPGSKGEVGADGAPGLPGLPGREGAAGPQGPKGEKGTQGEKGDPGKDGVGQPGLPGPPGPPGPVVYVSEQDRVLASVPGPEGRAGFAGFPGPAGPKGDLGAKGQQGIPGPKGEKGEPGTFFGPDGRALTPAQKGAKGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGMNGLKGEKGEPGDASVGFGMRGPAGPPGPPGPPGPPGTPVYDSNAFVDSGRPGPPGLPGHQGPSGLKGDKGDVGPPGPPGQFPFDLLQLGAEMKGEKGDRGAAGQKGERGEPGGGGFFGSGVPGPPGPPGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGPQGPPGPPGPPGPPGPPSFPGPYRQTISVPGPPGPPGPPGPPGSMGTSSGVRVWATYQMLVDQVPQVPEGWLIYVADREELYVRVRNGFRKVLLEAHVPLPHGTDNEVAVLQPPLVQLHEGNPYPRREVPHPTARSWRADDILASPPRLPHPQPYPGAPHHGAYAHLRPVPPTASPSHTHHDFQPVLHLVALNSPQSGGLRGIRGADFQCFQQARAAGLAGTFRAFLSSRLQDLYSIVRRADRATLPVVNLRDEVLFPSWEALFSGSEGQLKPGARIFSFDGRDVLQHPTWPQKSVWHGSDPSGRRLTESYCETWRTDSRAATGQASSLLAGRLLEQKAAGCHNAFIVLCIENSFMTSSSK

Target ORF information:

RefSeq Version	NM_001083388.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVIII alpha 1 chain (COL18A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083388.2

ORF Insert Sequence:

ATGGCTCCCG ACCCCAGCAG CGGCCGCGGC CACCACGGCC TCCTGCTGCT TGCCTGCTGC 
CTCTCGGCCG CCCAGGCTGA CCTGTTCTCC TGGATATGGT CTGGGAACAG TGCCTGGCCC 
ATAGCTGCGC CAGCCTCCAA GCCCCCGGAC AGCTCACCTG TGTGGCCCAC GGAGGATACC 
ACCACGCACG TGGCCCCCCG GGATGCTCCC ACCGACTGGT GGAAAGTCTC TGCCAGCCCC 
ACAGCCCCTC TGGGGAGGCC GGAGGTGGGG CAGGGCCAGA CCCCCACTGG CCCCACGGCC 
TCCACCGTGA GCCTGGACCC AAAGGAGGAA AACATCGCCG GCGTCGGAGC CAAGATCCTG 
AACGTGGCTC AGGGCATCCG GAGCTTTGTC CAGCTGTGGG ACAACGCTAA GGACACCACC 
CCTGCCGAGA GCTCTGCCGG GGCAGAGACG CCGCCCCCCA CGGACACCAT GGACGACCTC 
TCCACCCCTG GACCCTCCAT CACCCCCCAG GACAGTGGGA CCACCCTCCA GCTGAGCACC 
ACGGCCCTGA GCTCTCCAGA CACCCAGAGG ACTGAGGCCG GCACCCTGCC AATGCCCACC 
CAGCCGCCAC CCTCCCCCGG CAGACCCTGG TTCAGGGAGT CCTTGGTATC GCCGACCCCA 
GACGAAAAGG TCGTGGAGGT AGGGCTCCAG CAGCTGCTCG GGGAGCCCCC GCCCTGGCAG 
GTGGCCATGG TGCACGACCC CGACGTGGGG TCCGCCTATG AGTTCGGCGA GGACGGCAGC 
GGTGGAGGCC AGGCGGCCCG GAGACTCCTG CCTGGCACGT TCTTCCAGGA CTTCTCGCTG 
CTTGTGCGTG TGCGGCCGGC CTCGGCGCGT GCGGGCGTGC TCTTTGCGGT CACAGACCCG 
GCGCAGGCAG TGGTCTCGGT GGGCGTGAAG CTGACGGCGG CGCGTGGGGG GCGGCAGCAC 
ATGCAGCTGC TGTACACGGA GCCGGGAGCC ACCCACACAC GCACAGCCGC CAGCTTCGAG 
CTGCCCGCGC TGGATGGTCG CTGGACGCGC CTGGCACTCA GCGTGGATGG CGCCTATGCC 
AGGCTCTTCG TGGACTGTAA GGAGGTCCAG GGCCAGCCCC TGGCGCACTC GCTGCACGAC 
CTGCAGCTGG AGCCGGACGC CCGCCTCTTC GTGGCCCAGG CCGGTGGAGC AGACCCTGAA 
AAGTTCCAGG GTCTGATCTC AGAGCTGAGG TTGCGCAGGG ATCCTCAGGT GAGCCCCCGG 
CAGTGCCAGG ATGAGGACGA GGACGACGAT GATGATGGGG CGTCCGGAGA CCTGGGCAGC 
GGGCTCGTGG AGACCCAGGA GCATCTCCGG GAGGAGTTGG GTGCGCCCCT GGGGCCCAGG 
CTCCCTGAGG CTCCTCCGGT CACTTCTCCA CCCTTGGCCG GAGTCGGCAA CGAGGAGGAC 
TTGAGAACTG AGGAAGTAGA GGAGTCAACC ACAGCATCTT CATTAGGAGC TCAGACCCTC 
CCCAGCTTGG GCACAGTTGG CACGTGGGAT GAGAGTGTTT GGAGCCCTGG GAGCAGCGTG 
AAAGAGCGAG GCCTGAAGGG GCAGAAGGGA GAGCCTGGCG CTCAGGGCCC CCCTGGCCCC 
ATCGGCCCCC AGGGTCCTGC AGGCCCAGCC ATAGACAGCC ATGAGGCACA GCCTGTTTCT 
GGACCACAGG GACCCCCTGG GCCCCCGGGA CCCCCGGGGA AGGACGGCAC CCCTGGAAGG 
GACGGTGAGC CTGGTGATCC TGGTGAAGAC GGGAAGCCCG GGGACACTGG GCCACAGGGC 
TTCCCAGGGA CCCCAGGAGA CGTGGGCCCC AAGGGCGAGA AGGGGGATCC TGGAGTCGGA 
CCGAGGGGAC CCCCAGGACC CCAGGGGCCT CCGGGGCCCC CAGGGCCCTC CTTCAAACCC 
GACAGGCTGA CCTTCATCGA CATGGAGGGA TCCGGTTTTG GCGGCGACCT GGAGAGCCTC 
CGCGGCCCTC GCGGCTTCCC CGGCCCCCCA GGGCCCCCTG GCGTCCCAGG CCTGCCAGGA 
GAGCCAGGCC GCTTCGGGAT GAACAGCTCG GACGTCCCTG GACCCGCAGG CCTGCCCGGC 
GTGCCTGGGA GGGACGGGCC GCCTGGGCGT CCAGGGCCCC CGGGACCCCC AGGTACTCCA 
GGAAAAGACG GACAACCAGG ACTGACTGGA CAGAAAGGCA GCCTCGGTGA GGCAGGCGCC 
CCAGGACCCA AGGGAAGCAA GGGAGACCTT GGCCCCGTCG GCGCTCCTGG GGAGCATGGC 
CTGGCAGGAG CCCCTGGACC CGCAGGCCCA CCAGGGCCCC CTGGCCCCCC GGGGCCCCCG 
GGCCCAGGAT TCGCCGCAGG GTTTGACGAC ATGGAAGGCT CCGGGAGCCC CTTATGGTCA 
ACAGCCCGAG GTGCCGAGGG GCTGCAGGGA CCGCCTGGCA CGCCCGGAGT CAAGGGGGAT 
CCTGGAGCTG TGGGGCCACC AGGGAGCAAG GGAGAAGTTG GAGCAGACGG AGCACCGGGC 
CTCCCCGGCC TCCCGGGCAG AGAGGGTGCA GCTGGACCCC AGGGACCAAA AGGAGAAAAA 
GGCACTCAGG GAGAGAAAGG AGACCCAGGG AAGGATGGAG TGGGGCAGCC AGGACTCCCG 
GGACCCCCCG GACCCCCAGG GCCTGTCGTC TACGTGTCGG AGCAGGATAG AGTGCTGGCG 
AGCGTGCCAG GCCCCGAGGG CCGTGCAGGC TTCGCTGGCT TCCCCGGACC AGCCGGGCCA 
AAGGGAGACC TGGGCGCCAA AGGCCAGCAG GGCATCCCGG GACCCAAGGG TGAGAAGGGA 
GAGCCGGGCA CATTCTTCGG CCCCGATGGC AGAGCACTGA CCCCCGCCCA GAAAGGAGCC 
AAGGGTGAGC CTGGCTTCCG AGGACCTCCG GGTCCGTATG GGCGGCCCGG GCACAAGGGA 
GAGATCGGAT TCCCTGGACG GCCGGGGCGT CCCGGGATGA ACGGGCTGAA AGGGGAGAAG 
GGGGAGCCGG GGGACGCCAG CGTGGGATTC GGCATGAGGG GTCCAGCTGG CCCACCTGGG 
CCTCCGGGGC CCCCAGGCCC TCCCGGGACT CCTGTCTACG ACAGCAACGC ATTTGTGGAC 
TCTGGCCGCC CTGGACCCCC AGGATTGCCA GGGCACCAGG GGCCTTCTGG ACTCAAGGGT 
GACAAAGGAG ACGTGGGCCC GCCCGGACCG CCAGGGCAGT TCCCGTTTGA CCTCCTCCAG 
CTGGGTGCCG AGATGAAGGG GGAGAAGGGG GACCGAGGGG CCGCTGGGCA GAAAGGAGAA 
AGGGGTGAGC CCGGGGGCGG AGGATTCTTC GGCTCTGGTG TGCCTGGCCC CCCCGGCCCG 
CCTGGCTACC CAGGGATTCC GGGCCCCAAG GGAGAGAGCA TCCGCGGCCA GCCCGGCCCT 
CCGGGACCTC AGGGACCCCC TGGCATTGGC TACGAGGGAC CCCAGGGGCC TCCTGGGCCC 
CCCGGACCCC CAGGGCCCCC GGGACCCCCT TCCTTTCCTG GCCCCTACAG GCAGACCATC 
AGTGTTCCTG GCCCGCCAGG CCCACCTGGG CCCCCAGGAC CCCCTGGATC CATGGGCACT 
TCTTCAGGGG TGAGGGTCTG GGCCACGTAC CAGATGCTGG TGGACCAGGT GCCGCAGGTG 
CCGGAGGGCT GGCTCATCTA CGTGGCTGAC AGGGAGGAGC TGTACGTCCG GGTCCGGAAT 
GGGTTCCGGA AGGTTCTGCT GGAGGCCCAC GTGCCCCTCC CCCATGGGAC GGACAACGAG 
GTGGCCGTCT TGCAGCCCCC GCTGGTGCAG CTGCATGAGG GTAATCCCTA CCCCCGCCGG 
GAGGTTCCAC ACCCGACAGC GCGGTCCTGG CGGGCGGACG ACATCCTGGC CAGTCCCCCG 
CGCCTGCCGC ACCCCCAGCC CTACCCCGGC GCCCCGCACC ACGGCGCCTA CGCACACCTC 
CGGCCGGTGC CCCCCACGGC CTCGCCCTCC CACACCCACC ACGACTTCCA GCCGGTGCTG 
CACCTGGTGG CGCTCAACAG CCCGCAGTCG GGCGGCCTGC GCGGCATCCG CGGCGCCGAC 
TTTCAGTGCT TCCAGCAGGC GCGCGCCGCG GGGCTGGCCG GCACCTTCCG CGCGTTCCTG 
TCCTCGCGGT TGCAGGACCT GTACAGCATC GTGCGCCGCG CCGACCGTGC CACCCTGCCC 
GTCGTCAACC TCAGGGACGA GGTGCTGTTT CCTAGCTGGG AGGCCTTGTT CTCAGGCTCC 
GAGGGCCAGC TGAAGCCCGG CGCCCGCATC TTCTCCTTCG ACGGCAGAGA TGTCCTTCAG 
CATCCCACCT GGCCCCAGAA GAGCGTGTGG CACGGCTCAG ACCCCAGCGG GCGCCGGCTG 
ACCGAGAGCT ACTGCGAGAC GTGGCGGACG GACAGCAGGG CGGCCACGGG CCAGGCCTCC 
TCGCTGCTGG CGGGCCGGCT GCTGGAGCAG AAAGCCGCCG GGTGCCACAA CGCCTTCATT 
GTCCTCTGCA TCGAGAACAG CTTCATGACC TCCTCCTCCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Type XVIII collagen is newly transcribed during bovine adipogenesis.
Differentiation; research in biological diversity65(5)281-5(2000 May)
Inoue-Murayama M,Sugimoto Y,Niimi Y,Aso H

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COL18A1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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