SEC31B cDNA ORF clone, Bos taurus(cattle)

The following SEC31B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC31B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa230799 XM_024985556.1
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa88686 XM_015460649.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88687 XM_015460650.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88688 XM_015460651.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88689 XM_015460652.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88690 XM_015460653.1
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88691 XM_024985555.1
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88691 XM_015460654.1
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88692 XM_015460655.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88693 XM_015460656.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa33529 NM_001192161.2
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46979 XM_015460648.1
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46979 XM_010819848.3
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46981 XM_010819850.3
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46990 XM_010819860.3
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46991 XM_010819861.3
Latest version!
Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa88686 XM_015460649.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88689 XM_015460652.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa33529 NM_001192161.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46981 XM_010819850.2 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa46990 XM_010819860.2 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa88692 XM_015460655.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88693 XM_015460656.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa46979 XM_010819848.2 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa88688 XM_015460651.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa46991 XM_010819861.2 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa88687 XM_015460650.1 Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa230799
    Clone ID Related Accession (Same CDS sequence) XM_024985556.1
    Accession Version XM_024985556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGGACCTG ATGGAGAAAG TGATGGTCCT TAACAGGAGC
    TTGGAGCTAC TGCGGGGTCC TGATGGGGTG AGCCCAGGCC CTGCCACAAC CTACAGAATC
    ACTCAGTATG CCAACCTCCT GGCAGCCCAG GGCAGCCTGG CCACTGCCAT GAGCTACCTA
    CCCAGTGACT GTGCTCAGCT ACCAGTTCAG CAGCTGAGAG ATCGACTTTT TCATGCCCAG
    GGTTCTGGTG TCTTGGGCCA ACAGTCTCCC CCTTTCCCTT TTCCCCGGGT TGTCGTGGGA
    GCTACCCCCT ACTCTCAAGA GACATCCTCT CCCAGACTGA AATTCCAGTC TTCTCACCAG
    GTTCCAATTC TACCTCCAAG GCCAAGGATT TTTACACCTC AGTCATCACT AATGATGCCC
    TTGACACCTT CCCATCCTAG CCCTTATCAG GGCTCCAGAA TGCAGAATAC AAGTGACTAC
    AGGCCATCTG GGGTCCAGGA AGCACAGCCT TTGCCTCTGG GCCCTGGGGT AAGGCCTGCT
    TTATCTCAGC CTCAGCTGTT AGGAGGGCAA AGGGCACAAG CTCCTAACCC CGTGGGATTC
    CCTGGAACAT GGCCTCTCCT GGCTCCACCC ATGGCACCCC CAAACATTAT GCAGCCTGGC
    TCTGTCCCCC TGCCTAGGGC TCCTCCAGTG CACCCTCTGT TTCCTGTGAG AGCACCAGGC
    TTCAGCCCTA TGAGCTCCCA GCCACCAGCC CCTCCTGTCA GCTTCCCTGC GGCCCACCCT
    CCAGGAGGGC CAGGTACTCC ATGCGCTGGT GTCCTCCCGA CCACTGGCAT CTTGATTCCT
    CACCCAGGAC CTCAGGATTC CTTGAAAAAT GCTCCAGTGC CCAAGGGAAA CCTCCAGAGG
    AAAAAGTTGC CAGAGACATT TATGCCCCCA GCACCAATTA CTGCTCCAGT TATGTGCCTC
    ACCCCTGAGC CTCGAGGGGT CCTTTCCTCA CAACCTGCTG TCCCCAGTCA ATGTCAGCAG
    CCACTGGACA AGATCAACAG GAAGGAGCTT CCCCCTGAAC ACCAGTCTTT GAAGATCAGC
    TTTGAGGCAC TTCTGCAGCG CTGCTCCCTA TCTGCCACTG ATTTAAAGAC AAAACGGAAG
    CTAGATGAAG CTGCGCAACG TCTAGAATGT CTGTATGACA AGCTCTGCGA GGGGACAGTA
    AGTACACTCA GTGGTTCTCA TCTGCCTCCT GCTTTTCCAC CCTCACCCCT GGCCAAAGGG
    CACGCAGTGT CCCTTCCCAT AAGCCTCACT GACCCTGGGT TCCCTACAGA ATATAGACAT
    GTTGCCTGGA CTGGGCTCCA GAACTCCAGA AGGGAGTCAT CGCTTCTCCC TTCCATGCCT
    CCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841324.1
    CDS126..3074
    Translation

    Target ORF information:

    RefSeq Version XM_024985556.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGGACCTG ATGGAGAAAG TGATGGTCCT TAACAGGAGC
    TTGGAGCTAC TGCGGGGTCC TGATGGGGTG AGCCCAGGCC CTGCCACAAC CTACAGAATC
    ACTCAGTATG CCAACCTCCT GGCAGCCCAG GGCAGCCTGG CCACTGCCAT GAGCTACCTA
    CCCAGTGACT GTGCTCAGCT ACCAGTTCAG CAGCTGAGAG ATCGACTTTT TCATGCCCAG
    GGTTCTGGTG TCTTGGGCCA ACAGTCTCCC CCTTTCCCTT TTCCCCGGGT TGTCGTGGGA
    GCTACCCCCT ACTCTCAAGA GACATCCTCT CCCAGACTGA AATTCCAGTC TTCTCACCAG
    GTTCCAATTC TACCTCCAAG GCCAAGGATT TTTACACCTC AGTCATCACT AATGATGCCC
    TTGACACCTT CCCATCCTAG CCCTTATCAG GGCTCCAGAA TGCAGAATAC AAGTGACTAC
    AGGCCATCTG GGGTCCAGGA AGCACAGCCT TTGCCTCTGG GCCCTGGGGT AAGGCCTGCT
    TTATCTCAGC CTCAGCTGTT AGGAGGGCAA AGGGCACAAG CTCCTAACCC CGTGGGATTC
    CCTGGAACAT GGCCTCTCCT GGCTCCACCC ATGGCACCCC CAAACATTAT GCAGCCTGGC
    TCTGTCCCCC TGCCTAGGGC TCCTCCAGTG CACCCTCTGT TTCCTGTGAG AGCACCAGGC
    TTCAGCCCTA TGAGCTCCCA GCCACCAGCC CCTCCTGTCA GCTTCCCTGC GGCCCACCCT
    CCAGGAGGGC CAGGTACTCC ATGCGCTGGT GTCCTCCCGA CCACTGGCAT CTTGATTCCT
    CACCCAGGAC CTCAGGATTC CTTGAAAAAT GCTCCAGTGC CCAAGGGAAA CCTCCAGAGG
    AAAAAGTTGC CAGAGACATT TATGCCCCCA GCACCAATTA CTGCTCCAGT TATGTGCCTC
    ACCCCTGAGC CTCGAGGGGT CCTTTCCTCA CAACCTGCTG TCCCCAGTCA ATGTCAGCAG
    CCACTGGACA AGATCAACAG GAAGGAGCTT CCCCCTGAAC ACCAGTCTTT GAAGATCAGC
    TTTGAGGCAC TTCTGCAGCG CTGCTCCCTA TCTGCCACTG ATTTAAAGAC AAAACGGAAG
    CTAGATGAAG CTGCGCAACG TCTAGAATGT CTGTATGACA AGCTCTGCGA GGGGACAGTA
    AGTACACTCA GTGGTTCTCA TCTGCCTCCT GCTTTTCCAC CCTCACCCCT GGCCAAAGGG
    CACGCAGTGT CCCTTCCCAT AAGCCTCACT GACCCTGGGT TCCCTACAGA ATATAGACAT
    GTTGCCTGGA CTGGGCTCCA GAACTCCAGA AGGGAGTCAT CGCTTCTCCC TTCCATGCCT
    CCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88686
    Clone ID Related Accession (Same CDS sequence) XM_015460649.1 , XM_015460649.2
    Accession Version XM_015460649.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316135.1
    CDS103..3558
    Translation

    Target ORF information:

    RefSeq Version XM_015460649.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88686
    Clone ID Related Accession (Same CDS sequence) XM_015460649.1 , XM_015460649.2
    Accession Version XM_015460649.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316135.1
    CDS126..3581
    Translation

    Target ORF information:

    RefSeq Version XM_015460649.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88687
    Clone ID Related Accession (Same CDS sequence) XM_015460650.1 , XM_015460650.2
    Accession Version XM_015460650.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGCA ATGTCAGCAG CCACTGGACA AGATCAACAG GAAGGAGCTT
    CCCCCTGAAC ACCAGTCTTT GAAGATCAGC TTTGAGGCAC TTCTGCAGCG CTGCTCCCTA
    TCTGCCACTG ATTTAAAGAC AAAACGGAAG CTAGATGAAG CTGCGCAACG TCTAGAATGT
    CTGTATGACA AGCTCTGCGA GGGGACAGTA AGTACACTCA GTGGTTCTCA TCTGCCTCCT
    GCTTTTCCAC CCTCACCCCT GGCCAAAGGG CACGCAGTGT CCCTTCCCAT AAGCCTCACT
    GACCCTGGGT TCCCTACAGA ATATAGACAT GTTGCCTGGA CTGGGCTCCA GAACTCCAGA
    AGGGAGTCAT CGCTTCTCCC TTCCATGCCT CCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316136.1
    CDS103..3501
    Translation

    Target ORF information:

    RefSeq Version XM_015460650.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGCA ATGTCAGCAG CCACTGGACA AGATCAACAG GAAGGAGCTT
    CCCCCTGAAC ACCAGTCTTT GAAGATCAGC TTTGAGGCAC TTCTGCAGCG CTGCTCCCTA
    TCTGCCACTG ATTTAAAGAC AAAACGGAAG CTAGATGAAG CTGCGCAACG TCTAGAATGT
    CTGTATGACA AGCTCTGCGA GGGGACAGTA AGTACACTCA GTGGTTCTCA TCTGCCTCCT
    GCTTTTCCAC CCTCACCCCT GGCCAAAGGG CACGCAGTGT CCCTTCCCAT AAGCCTCACT
    GACCCTGGGT TCCCTACAGA ATATAGACAT GTTGCCTGGA CTGGGCTCCA GAACTCCAGA
    AGGGAGTCAT CGCTTCTCCC TTCCATGCCT CCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88687
    Clone ID Related Accession (Same CDS sequence) XM_015460650.1 , XM_015460650.2
    Accession Version XM_015460650.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGCA ATGTCAGCAG CCACTGGACA AGATCAACAG GAAGGAGCTT
    CCCCCTGAAC ACCAGTCTTT GAAGATCAGC TTTGAGGCAC TTCTGCAGCG CTGCTCCCTA
    TCTGCCACTG ATTTAAAGAC AAAACGGAAG CTAGATGAAG CTGCGCAACG TCTAGAATGT
    CTGTATGACA AGCTCTGCGA GGGGACAGTA AGTACACTCA GTGGTTCTCA TCTGCCTCCT
    GCTTTTCCAC CCTCACCCCT GGCCAAAGGG CACGCAGTGT CCCTTCCCAT AAGCCTCACT
    GACCCTGGGT TCCCTACAGA ATATAGACAT GTTGCCTGGA CTGGGCTCCA GAACTCCAGA
    AGGGAGTCAT CGCTTCTCCC TTCCATGCCT CCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316136.1
    CDS126..3524
    Translation

    Target ORF information:

    RefSeq Version XM_015460650.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460650.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGCA ATGTCAGCAG CCACTGGACA AGATCAACAG GAAGGAGCTT
    CCCCCTGAAC ACCAGTCTTT GAAGATCAGC TTTGAGGCAC TTCTGCAGCG CTGCTCCCTA
    TCTGCCACTG ATTTAAAGAC AAAACGGAAG CTAGATGAAG CTGCGCAACG TCTAGAATGT
    CTGTATGACA AGCTCTGCGA GGGGACAGTA AGTACACTCA GTGGTTCTCA TCTGCCTCCT
    GCTTTTCCAC CCTCACCCCT GGCCAAAGGG CACGCAGTGT CCCTTCCCAT AAGCCTCACT
    GACCCTGGGT TCCCTACAGA ATATAGACAT GTTGCCTGGA CTGGGCTCCA GAACTCCAGA
    AGGGAGTCAT CGCTTCTCCC TTCCATGCCT CCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88688
    Clone ID Related Accession (Same CDS sequence) XM_015460651.2 , XM_015460651.1
    Accession Version XM_015460651.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAT CTGCAGATTG CCTCTATGGG AGCCAACATA AATATTTCTC CTCCAGGAAT
    GCGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316137.1
    CDS126..3494
    Translation

    Target ORF information:

    RefSeq Version XM_015460651.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460651.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAT CTGCAGATTG CCTCTATGGG AGCCAACATA AATATTTCTC CTCCAGGAAT
    GCGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88688
    Clone ID Related Accession (Same CDS sequence) XM_015460651.2 , XM_015460651.1
    Accession Version XM_015460651.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAT CTGCAGATTG CCTCTATGGG AGCCAACATA AATATTTCTC CTCCAGGAAT
    GCGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316137.1
    CDS103..3471
    Translation

    Target ORF information:

    RefSeq Version XM_015460651.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAT CTGCAGATTG CCTCTATGGG AGCCAACATA AATATTTCTC CTCCAGGAAT
    GCGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88689
    Clone ID Related Accession (Same CDS sequence) XM_015460652.1 , XM_015460652.2
    Accession Version XM_015460652.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGTTTGGG GGCAAGCTGG TGACCTTTGG CCTCCCCAGC
    ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC CTCCGCCTAG TCTTTATCAG TCAGGTCACC
    ACAGAACCTG AACTCCTGGC ACAGTCAGCT GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT
    CTCCTGAATT ACTGTCAGAA CAAGATCCAC CAAGCATCAC TGCAAAGTGA AAAGATGCTC
    TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG GACGCCAGGA TGAAGTTCCT GAAGCTATTA
    GGATACAGTA AAGATGAACT TCAGAAGAAG GTGGCCACAT GGTTGAGAAG TGATTTGAGG
    CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT GACCTCAACA GTAACAGACA ACAGGCCTTC
    TCCATACAGA CCTCCAAACA TACCACCTGG GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT
    GAGCTGATCC CTCAAAACAT GACTCTGTGG GAGATCCCCA TCACAGAAGA CACGGAGGGA
    CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG
    GAAGAGCGCT TTGCTGATGC CATCATCCTG GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG
    CAAGCACAGG AGCGCTACTT GGCCAAGAAG AAAACCGGAA TCGCCCCGCT GCTAGCCTGC
    ATTATACAGA AGAATTGGAA AGACATGGTG CATGCCTGTA GCCTGAAGAA CTGGAGAGAG
    GCTCTGGCCT TGCTGCTGAC ATACTCAGAG CCAGAGAAAT TCCCCGAGCT CTGTGACAGG
    CTGGGAACTC GTATGGAGCA GGAGAGTGGC TGGGCACTCA CCTCTGAAGC CAGACTCTGT
    TATGTGTGCT CAGGGAGTGT GGAGCGGCTG GTGGAGTGCT GGGCAAAATG CCAGCCGGCT
    TCATCCCCCA TGGCTTTACA GGACCTGATG GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG
    GAGCTACTGC GGGGTCCTGA TGGGGTGAGC CCAGGCCCTG CCACAACCTA CAGAATCACT
    CAGTATGCCA ACCTCCTGGC AGCCCAGGGC AGCCTGGCCA CTGCCATGAG CTACCTACCC
    AGTGACTGTG CTCAGCTACC AGTTCAGCAG CTGAGAGATC GACTTTTTCA TGCCCAGGGT
    TCTGGTGTCT TGGGCCAACA GTCTCCCCCT TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT
    ACCCCCTACT CTCAAGAGAC ATCCTCTCCC AGACTGAAAT TCCAGTCTTC TCACCAGGTT
    CCAATTCTAC CTCCAAGGCC AAGGATTTTT ACACCTCAGT CATCACTAAT GATGCCCTTG
    ACACCTTCCC ATCCTAGCCC TTATCAGGGC TCCAGAATGC AGAATACAAG TGACTACAGG
    CCATCTGGGG TCCAGGAAGC ACAGCCTTTG CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA
    TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG GCACAAGCTC CTAACCCCGT GGGATTCCCT
    GGAACATGGC CTCTCCTGGC TCCACCCATG GCACCCCCAA ACATTATGCA GCCTGGCTCT
    GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC
    AGCCCTATGA GCTCCCAGCC ACCAGCCCCT CCTGTCAGCT TCCCTGCGGC CCACCCTCCA
    GGAGGGCCAG GTACTCCATG CGCTGGTGTC CTCCCGACCA CTGGCATCTT GATTCCTCAC
    CCAGGACCTC AGGATTCCTT GAAAAATGCT CCAGTGCCCA AGGGAAACCT CCAGAGGAAA
    AAGTTGCCAG AGACATTTAT GCCCCCAGCA CCAATTACTG CTCCAGTTAT GTGCCTCACC
    CCTGAGCCTC GAGGGGTCCT TTCCTCACAA CCTGCTGTCC CCAGTCAATG TCAGCAGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACAGTAAGT
    ACACTCAGTG GTTCTCATCT GCCTCCTGCT TTTCCACCCT CACCCCTGGC CAAAGGGCAC
    GCAGTGTCCC TTCCCATAAG CCTCACTGAC CCTGGGTTCC CTACAGAATA TAGACATGTT
    GCCTGGACTG GGCTCCAGAA CTCCAGAAGG GAGTCATCGC TTCTCCCTTC CATGCCTCCA
    GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316138.1
    CDS103..3468
    Translation

    Target ORF information:

    RefSeq Version XM_015460652.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGTTTGGG GGCAAGCTGG TGACCTTTGG CCTCCCCAGC
    ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC CTCCGCCTAG TCTTTATCAG TCAGGTCACC
    ACAGAACCTG AACTCCTGGC ACAGTCAGCT GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT
    CTCCTGAATT ACTGTCAGAA CAAGATCCAC CAAGCATCAC TGCAAAGTGA AAAGATGCTC
    TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG GACGCCAGGA TGAAGTTCCT GAAGCTATTA
    GGATACAGTA AAGATGAACT TCAGAAGAAG GTGGCCACAT GGTTGAGAAG TGATTTGAGG
    CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT GACCTCAACA GTAACAGACA ACAGGCCTTC
    TCCATACAGA CCTCCAAACA TACCACCTGG GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT
    GAGCTGATCC CTCAAAACAT GACTCTGTGG GAGATCCCCA TCACAGAAGA CACGGAGGGA
    CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG
    GAAGAGCGCT TTGCTGATGC CATCATCCTG GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG
    CAAGCACAGG AGCGCTACTT GGCCAAGAAG AAAACCGGAA TCGCCCCGCT GCTAGCCTGC
    ATTATACAGA AGAATTGGAA AGACATGGTG CATGCCTGTA GCCTGAAGAA CTGGAGAGAG
    GCTCTGGCCT TGCTGCTGAC ATACTCAGAG CCAGAGAAAT TCCCCGAGCT CTGTGACAGG
    CTGGGAACTC GTATGGAGCA GGAGAGTGGC TGGGCACTCA CCTCTGAAGC CAGACTCTGT
    TATGTGTGCT CAGGGAGTGT GGAGCGGCTG GTGGAGTGCT GGGCAAAATG CCAGCCGGCT
    TCATCCCCCA TGGCTTTACA GGACCTGATG GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG
    GAGCTACTGC GGGGTCCTGA TGGGGTGAGC CCAGGCCCTG CCACAACCTA CAGAATCACT
    CAGTATGCCA ACCTCCTGGC AGCCCAGGGC AGCCTGGCCA CTGCCATGAG CTACCTACCC
    AGTGACTGTG CTCAGCTACC AGTTCAGCAG CTGAGAGATC GACTTTTTCA TGCCCAGGGT
    TCTGGTGTCT TGGGCCAACA GTCTCCCCCT TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT
    ACCCCCTACT CTCAAGAGAC ATCCTCTCCC AGACTGAAAT TCCAGTCTTC TCACCAGGTT
    CCAATTCTAC CTCCAAGGCC AAGGATTTTT ACACCTCAGT CATCACTAAT GATGCCCTTG
    ACACCTTCCC ATCCTAGCCC TTATCAGGGC TCCAGAATGC AGAATACAAG TGACTACAGG
    CCATCTGGGG TCCAGGAAGC ACAGCCTTTG CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA
    TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG GCACAAGCTC CTAACCCCGT GGGATTCCCT
    GGAACATGGC CTCTCCTGGC TCCACCCATG GCACCCCCAA ACATTATGCA GCCTGGCTCT
    GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC
    AGCCCTATGA GCTCCCAGCC ACCAGCCCCT CCTGTCAGCT TCCCTGCGGC CCACCCTCCA
    GGAGGGCCAG GTACTCCATG CGCTGGTGTC CTCCCGACCA CTGGCATCTT GATTCCTCAC
    CCAGGACCTC AGGATTCCTT GAAAAATGCT CCAGTGCCCA AGGGAAACCT CCAGAGGAAA
    AAGTTGCCAG AGACATTTAT GCCCCCAGCA CCAATTACTG CTCCAGTTAT GTGCCTCACC
    CCTGAGCCTC GAGGGGTCCT TTCCTCACAA CCTGCTGTCC CCAGTCAATG TCAGCAGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACAGTAAGT
    ACACTCAGTG GTTCTCATCT GCCTCCTGCT TTTCCACCCT CACCCCTGGC CAAAGGGCAC
    GCAGTGTCCC TTCCCATAAG CCTCACTGAC CCTGGGTTCC CTACAGAATA TAGACATGTT
    GCCTGGACTG GGCTCCAGAA CTCCAGAAGG GAGTCATCGC TTCTCCCTTC CATGCCTCCA
    GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88689
    Clone ID Related Accession (Same CDS sequence) XM_015460652.1 , XM_015460652.2
    Accession Version XM_015460652.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGTTTGGG GGCAAGCTGG TGACCTTTGG CCTCCCCAGC
    ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC CTCCGCCTAG TCTTTATCAG TCAGGTCACC
    ACAGAACCTG AACTCCTGGC ACAGTCAGCT GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT
    CTCCTGAATT ACTGTCAGAA CAAGATCCAC CAAGCATCAC TGCAAAGTGA AAAGATGCTC
    TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG GACGCCAGGA TGAAGTTCCT GAAGCTATTA
    GGATACAGTA AAGATGAACT TCAGAAGAAG GTGGCCACAT GGTTGAGAAG TGATTTGAGG
    CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT GACCTCAACA GTAACAGACA ACAGGCCTTC
    TCCATACAGA CCTCCAAACA TACCACCTGG GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT
    GAGCTGATCC CTCAAAACAT GACTCTGTGG GAGATCCCCA TCACAGAAGA CACGGAGGGA
    CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG
    GAAGAGCGCT TTGCTGATGC CATCATCCTG GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG
    CAAGCACAGG AGCGCTACTT GGCCAAGAAG AAAACCGGAA TCGCCCCGCT GCTAGCCTGC
    ATTATACAGA AGAATTGGAA AGACATGGTG CATGCCTGTA GCCTGAAGAA CTGGAGAGAG
    GCTCTGGCCT TGCTGCTGAC ATACTCAGAG CCAGAGAAAT TCCCCGAGCT CTGTGACAGG
    CTGGGAACTC GTATGGAGCA GGAGAGTGGC TGGGCACTCA CCTCTGAAGC CAGACTCTGT
    TATGTGTGCT CAGGGAGTGT GGAGCGGCTG GTGGAGTGCT GGGCAAAATG CCAGCCGGCT
    TCATCCCCCA TGGCTTTACA GGACCTGATG GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG
    GAGCTACTGC GGGGTCCTGA TGGGGTGAGC CCAGGCCCTG CCACAACCTA CAGAATCACT
    CAGTATGCCA ACCTCCTGGC AGCCCAGGGC AGCCTGGCCA CTGCCATGAG CTACCTACCC
    AGTGACTGTG CTCAGCTACC AGTTCAGCAG CTGAGAGATC GACTTTTTCA TGCCCAGGGT
    TCTGGTGTCT TGGGCCAACA GTCTCCCCCT TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT
    ACCCCCTACT CTCAAGAGAC ATCCTCTCCC AGACTGAAAT TCCAGTCTTC TCACCAGGTT
    CCAATTCTAC CTCCAAGGCC AAGGATTTTT ACACCTCAGT CATCACTAAT GATGCCCTTG
    ACACCTTCCC ATCCTAGCCC TTATCAGGGC TCCAGAATGC AGAATACAAG TGACTACAGG
    CCATCTGGGG TCCAGGAAGC ACAGCCTTTG CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA
    TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG GCACAAGCTC CTAACCCCGT GGGATTCCCT
    GGAACATGGC CTCTCCTGGC TCCACCCATG GCACCCCCAA ACATTATGCA GCCTGGCTCT
    GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC
    AGCCCTATGA GCTCCCAGCC ACCAGCCCCT CCTGTCAGCT TCCCTGCGGC CCACCCTCCA
    GGAGGGCCAG GTACTCCATG CGCTGGTGTC CTCCCGACCA CTGGCATCTT GATTCCTCAC
    CCAGGACCTC AGGATTCCTT GAAAAATGCT CCAGTGCCCA AGGGAAACCT CCAGAGGAAA
    AAGTTGCCAG AGACATTTAT GCCCCCAGCA CCAATTACTG CTCCAGTTAT GTGCCTCACC
    CCTGAGCCTC GAGGGGTCCT TTCCTCACAA CCTGCTGTCC CCAGTCAATG TCAGCAGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACAGTAAGT
    ACACTCAGTG GTTCTCATCT GCCTCCTGCT TTTCCACCCT CACCCCTGGC CAAAGGGCAC
    GCAGTGTCCC TTCCCATAAG CCTCACTGAC CCTGGGTTCC CTACAGAATA TAGACATGTT
    GCCTGGACTG GGCTCCAGAA CTCCAGAAGG GAGTCATCGC TTCTCCCTTC CATGCCTCCA
    GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316138.1
    CDS126..3491
    Translation

    Target ORF information:

    RefSeq Version XM_015460652.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460652.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGTTTGGG GGCAAGCTGG TGACCTTTGG CCTCCCCAGC
    ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC CTCCGCCTAG TCTTTATCAG TCAGGTCACC
    ACAGAACCTG AACTCCTGGC ACAGTCAGCT GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT
    CTCCTGAATT ACTGTCAGAA CAAGATCCAC CAAGCATCAC TGCAAAGTGA AAAGATGCTC
    TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG GACGCCAGGA TGAAGTTCCT GAAGCTATTA
    GGATACAGTA AAGATGAACT TCAGAAGAAG GTGGCCACAT GGTTGAGAAG TGATTTGAGG
    CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT GACCTCAACA GTAACAGACA ACAGGCCTTC
    TCCATACAGA CCTCCAAACA TACCACCTGG GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT
    GAGCTGATCC CTCAAAACAT GACTCTGTGG GAGATCCCCA TCACAGAAGA CACGGAGGGA
    CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG
    GAAGAGCGCT TTGCTGATGC CATCATCCTG GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG
    CAAGCACAGG AGCGCTACTT GGCCAAGAAG AAAACCGGAA TCGCCCCGCT GCTAGCCTGC
    ATTATACAGA AGAATTGGAA AGACATGGTG CATGCCTGTA GCCTGAAGAA CTGGAGAGAG
    GCTCTGGCCT TGCTGCTGAC ATACTCAGAG CCAGAGAAAT TCCCCGAGCT CTGTGACAGG
    CTGGGAACTC GTATGGAGCA GGAGAGTGGC TGGGCACTCA CCTCTGAAGC CAGACTCTGT
    TATGTGTGCT CAGGGAGTGT GGAGCGGCTG GTGGAGTGCT GGGCAAAATG CCAGCCGGCT
    TCATCCCCCA TGGCTTTACA GGACCTGATG GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG
    GAGCTACTGC GGGGTCCTGA TGGGGTGAGC CCAGGCCCTG CCACAACCTA CAGAATCACT
    CAGTATGCCA ACCTCCTGGC AGCCCAGGGC AGCCTGGCCA CTGCCATGAG CTACCTACCC
    AGTGACTGTG CTCAGCTACC AGTTCAGCAG CTGAGAGATC GACTTTTTCA TGCCCAGGGT
    TCTGGTGTCT TGGGCCAACA GTCTCCCCCT TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT
    ACCCCCTACT CTCAAGAGAC ATCCTCTCCC AGACTGAAAT TCCAGTCTTC TCACCAGGTT
    CCAATTCTAC CTCCAAGGCC AAGGATTTTT ACACCTCAGT CATCACTAAT GATGCCCTTG
    ACACCTTCCC ATCCTAGCCC TTATCAGGGC TCCAGAATGC AGAATACAAG TGACTACAGG
    CCATCTGGGG TCCAGGAAGC ACAGCCTTTG CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA
    TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG GCACAAGCTC CTAACCCCGT GGGATTCCCT
    GGAACATGGC CTCTCCTGGC TCCACCCATG GCACCCCCAA ACATTATGCA GCCTGGCTCT
    GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC
    AGCCCTATGA GCTCCCAGCC ACCAGCCCCT CCTGTCAGCT TCCCTGCGGC CCACCCTCCA
    GGAGGGCCAG GTACTCCATG CGCTGGTGTC CTCCCGACCA CTGGCATCTT GATTCCTCAC
    CCAGGACCTC AGGATTCCTT GAAAAATGCT CCAGTGCCCA AGGGAAACCT CCAGAGGAAA
    AAGTTGCCAG AGACATTTAT GCCCCCAGCA CCAATTACTG CTCCAGTTAT GTGCCTCACC
    CCTGAGCCTC GAGGGGTCCT TTCCTCACAA CCTGCTGTCC CCAGTCAATG TCAGCAGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACAGTAAGT
    ACACTCAGTG GTTCTCATCT GCCTCCTGCT TTTCCACCCT CACCCCTGGC CAAAGGGCAC
    GCAGTGTCCC TTCCCATAAG CCTCACTGAC CCTGGGTTCC CTACAGAATA TAGACATGTT
    GCCTGGACTG GGCTCCAGAA CTCCAGAAGG GAGTCATCGC TTCTCCCTTC CATGCCTCCA
    GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88690
    Clone ID Related Accession (Same CDS sequence) XM_015460653.1
    Accession Version XM_015460653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGCTGCTA GCCTGCATTA TACAGAAGAA TTGGAAAGAC
    ATGGTGCATG CCTGTAGCCT GAAGAACTGG AGAGAGGCTC TGGCCTTGCT GCTGACATAC
    TCAGAGCCAG AGAAATTCCC CGAGCTCTGT GACAGGCTGG GAACTCGTAT GGAGCAGGAG
    AGTGGCTGGG CACTCACCTC TGAAGCCAGA CTCTGTTATG TGTGCTCAGG GAGTGTGGAG
    CGGCTGGTGG AGTGCTGGGC AAAATGCCAG CCGGCTTCAT CCCCCATGGC TTTACAGGAC
    CTGATGGAGA AAGTGATGGT CCTTAACAGG AGCTTGGAGC TACTGCGGGG TCCTGATGGG
    GTGAGCCCAG GCCCTGCCAC AACCTACAGA ATCACTCAGT ATGCCAACCT CCTGGCAGCC
    CAGGGCAGCC TGGCCACTGC CATGAGCTAC CTACCCAGTG ACTGTGCTCA GCTACCAGTT
    CAGCAGCTGA GAGATCGACT TTTTCATGCC CAGGGTTCTG GTGTCTTGGG CCAACAGTCT
    CCCCCTTTCC CTTTTCCCCG GGTTGTCGTG GGAGCTACCC CCTACTCTCA AGAGACATCC
    TCTCCCAGAC TGAAATTCCA GTCTTCTCAC CAGGTTCCAA TTCTACCTCC AAGGCCAAGG
    ATTTTTACAC CTCAGTCATC ACTAATGATG CCCTTGACAC CTTCCCATCC TAGCCCTTAT
    CAGGGCTCCA GAATGCAGAA TACAAGTGAC TACAGGCCAT CTGGGGTCCA GGAAGCACAG
    CCTTTGCCTC TGGGCCCTGG GGTAAGGCCT GCTTTATCTC AGCCTCAGCT GTTAGGAGGG
    CAAAGGGCAC AAGCTCCTAA CCCCGTGGGA TTCCCTGGAA CATGGCCTCT CCTGGCTCCA
    CCCATGGCAC CCCCAAACAT TATGCAGCCT GGCTCTGTCC CCCTGCCTAG GGCTCCTCCA
    GTGCACCCTC TGTTTCCTGT GAGAGCACCA GGCTTCAGCC CTATGAGCTC CCAGCCACCA
    GCCCCTCCTG TCAGCTTCCC TGCGGCCCAC CCTCCAGGAG GGCCAGGTAC TCCATGCGCT
    GGTGTCCTCC CGACCACTGG CATCTTGATT CCTCACCCAG GACCTCAGGA TTCCTTGAAA
    AATGCTCCAG TGCCCAAGGG AAACCTCCAG AGGAAAAAGT TGCCAGAGAC ATTTATGCCC
    CCAGCACCAA TTACTGCTCC AGTTATGTGC CTCACCCCTG AGCCTCGAGG GGTCCTTTCC
    TCACAACCTG CTGTCCCCAG TCAATGTCAG CAGCCACTGG ACAAGATCAA CAGGAAGGAG
    CTTCCCCCTG AACACCAGTC TTTGAAGATC AGCTTTGAGG CACTTCTGCA GCGCTGCTCC
    CTATCTGCCA CTGATTTAAA GACAAAACGG AAGCTAGATG AAGCTGCGCA ACGTCTAGAA
    TGTCTGTATG ACAAGCTCTG CGAGGGGACA GTAAGTACAC TCAGTGGTTC TCATCTGCCT
    CCTGCTTTTC CACCCTCACC CCTGGCCAAA GGGCACGCAG TGTCCCTTCC CATAAGCCTC
    ACTGACCCTG GGTTCCCTAC AGAATATAGA CATGTTGCCT GGACTGGGCT CCAGAACTCC
    AGAAGGGAGT CATCGCTTCT CCCTTCCATG CCTCCAGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316139.1
    CDS103..3324
    Translation

    Target ORF information:

    RefSeq Version XM_015460653.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGCTGCTA GCCTGCATTA TACAGAAGAA TTGGAAAGAC
    ATGGTGCATG CCTGTAGCCT GAAGAACTGG AGAGAGGCTC TGGCCTTGCT GCTGACATAC
    TCAGAGCCAG AGAAATTCCC CGAGCTCTGT GACAGGCTGG GAACTCGTAT GGAGCAGGAG
    AGTGGCTGGG CACTCACCTC TGAAGCCAGA CTCTGTTATG TGTGCTCAGG GAGTGTGGAG
    CGGCTGGTGG AGTGCTGGGC AAAATGCCAG CCGGCTTCAT CCCCCATGGC TTTACAGGAC
    CTGATGGAGA AAGTGATGGT CCTTAACAGG AGCTTGGAGC TACTGCGGGG TCCTGATGGG
    GTGAGCCCAG GCCCTGCCAC AACCTACAGA ATCACTCAGT ATGCCAACCT CCTGGCAGCC
    CAGGGCAGCC TGGCCACTGC CATGAGCTAC CTACCCAGTG ACTGTGCTCA GCTACCAGTT
    CAGCAGCTGA GAGATCGACT TTTTCATGCC CAGGGTTCTG GTGTCTTGGG CCAACAGTCT
    CCCCCTTTCC CTTTTCCCCG GGTTGTCGTG GGAGCTACCC CCTACTCTCA AGAGACATCC
    TCTCCCAGAC TGAAATTCCA GTCTTCTCAC CAGGTTCCAA TTCTACCTCC AAGGCCAAGG
    ATTTTTACAC CTCAGTCATC ACTAATGATG CCCTTGACAC CTTCCCATCC TAGCCCTTAT
    CAGGGCTCCA GAATGCAGAA TACAAGTGAC TACAGGCCAT CTGGGGTCCA GGAAGCACAG
    CCTTTGCCTC TGGGCCCTGG GGTAAGGCCT GCTTTATCTC AGCCTCAGCT GTTAGGAGGG
    CAAAGGGCAC AAGCTCCTAA CCCCGTGGGA TTCCCTGGAA CATGGCCTCT CCTGGCTCCA
    CCCATGGCAC CCCCAAACAT TATGCAGCCT GGCTCTGTCC CCCTGCCTAG GGCTCCTCCA
    GTGCACCCTC TGTTTCCTGT GAGAGCACCA GGCTTCAGCC CTATGAGCTC CCAGCCACCA
    GCCCCTCCTG TCAGCTTCCC TGCGGCCCAC CCTCCAGGAG GGCCAGGTAC TCCATGCGCT
    GGTGTCCTCC CGACCACTGG CATCTTGATT CCTCACCCAG GACCTCAGGA TTCCTTGAAA
    AATGCTCCAG TGCCCAAGGG AAACCTCCAG AGGAAAAAGT TGCCAGAGAC ATTTATGCCC
    CCAGCACCAA TTACTGCTCC AGTTATGTGC CTCACCCCTG AGCCTCGAGG GGTCCTTTCC
    TCACAACCTG CTGTCCCCAG TCAATGTCAG CAGCCACTGG ACAAGATCAA CAGGAAGGAG
    CTTCCCCCTG AACACCAGTC TTTGAAGATC AGCTTTGAGG CACTTCTGCA GCGCTGCTCC
    CTATCTGCCA CTGATTTAAA GACAAAACGG AAGCTAGATG AAGCTGCGCA ACGTCTAGAA
    TGTCTGTATG ACAAGCTCTG CGAGGGGACA GTAAGTACAC TCAGTGGTTC TCATCTGCCT
    CCTGCTTTTC CACCCTCACC CCTGGCCAAA GGGCACGCAG TGTCCCTTCC CATAAGCCTC
    ACTGACCCTG GGTTCCCTAC AGAATATAGA CATGTTGCCT GGACTGGGCT CCAGAACTCC
    AGAAGGGAGT CATCGCTTCT CCCTTCCATG CCTCCAGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88691
    Clone ID Related Accession (Same CDS sequence) XM_024985555.1 , XM_015460654.1
    Accession Version XM_024985555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTTACTC TGTACAATGT GACCCACGTC TTGTCTTCGG GAAAGGAGCC TGTGATTGCC 
    CAGAGACAGA AGCACTCTGG GGCTGTCAGA GCCCTTGACT TTAATCCTTT CCAGGGCAAC
    CTCCTGGCCT CAGGGGCCAA TGATTCTGAA ATCTTCATTT GGGATTTGAA TAACCTGAGT
    GTACCAATGA CCCCAGGATC CAAGTCACAG CAGCCCCTGG AAGATATCAA GGCTCTCTCT
    TGGAACCGTC AAGTTCAACA CATTCTGTCT TCTGCTCACC CCAGCGGCAA GGCAGTCGTG
    TGGGATCTCA GGAAGAATGA ACCTATCATC AAAGTCAGTG ATCACAGCAA CCGGATGCAC
    TGCTCAGGCC TGGCCTGGCA CCCAGACATC GCCACCCAGT TGGTGCTTTG TTCAGAAGAT
    GATCGTCTTC CAGTAATTCA GCTGTGGGAC TTGCGCTTTG CCTCCTCACC CCTAAAGGTG
    CTGGAGAGTC ACAGCAGGGG GATCCTGTCA GTGTCATGGA GTCAGGCTGA TGCTGAGCTG
    CTGCTCAGTA GTGCCAAGGA CAGCCAGATC TTGTGCTGGA ACCTGGGGAG CAGTGAGGTG
    GTGTATAAAC TACCCATGCT GAGCAGCTGG TGCTTTGATG TGCAATGGTG CCCTCGGGAC
    CCTCTGGTGT TCTCTGCTGC TTCCTTTGAT GGCTGGATCA ATTTATACTC TGTGATGGGT
    AGGAGCTGGG AAGTCCAGCA GATGAGACAG GCTGACAAGA TCTCATCTTC CTTCAGCAAA
    GGCCAGCCTC TCCCACCATT GCAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841323.1
    CDS267..3482
    Translation

    Target ORF information:

    RefSeq Version XM_024985555.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTTACTC TGTACAATGT GACCCACGTC TTGTCTTCGG GAAAGGAGCC TGTGATTGCC 
    CAGAGACAGA AGCACTCTGG GGCTGTCAGA GCCCTTGACT TTAATCCTTT CCAGGGCAAC
    CTCCTGGCCT CAGGGGCCAA TGATTCTGAA ATCTTCATTT GGGATTTGAA TAACCTGAGT
    GTACCAATGA CCCCAGGATC CAAGTCACAG CAGCCCCTGG AAGATATCAA GGCTCTCTCT
    TGGAACCGTC AAGTTCAACA CATTCTGTCT TCTGCTCACC CCAGCGGCAA GGCAGTCGTG
    TGGGATCTCA GGAAGAATGA ACCTATCATC AAAGTCAGTG ATCACAGCAA CCGGATGCAC
    TGCTCAGGCC TGGCCTGGCA CCCAGACATC GCCACCCAGT TGGTGCTTTG TTCAGAAGAT
    GATCGTCTTC CAGTAATTCA GCTGTGGGAC TTGCGCTTTG CCTCCTCACC CCTAAAGGTG
    CTGGAGAGTC ACAGCAGGGG GATCCTGTCA GTGTCATGGA GTCAGGCTGA TGCTGAGCTG
    CTGCTCAGTA GTGCCAAGGA CAGCCAGATC TTGTGCTGGA ACCTGGGGAG CAGTGAGGTG
    GTGTATAAAC TACCCATGCT GAGCAGCTGG TGCTTTGATG TGCAATGGTG CCCTCGGGAC
    CCTCTGGTGT TCTCTGCTGC TTCCTTTGAT GGCTGGATCA ATTTATACTC TGTGATGGGT
    AGGAGCTGGG AAGTCCAGCA GATGAGACAG GCTGACAAGA TCTCATCTTC CTTCAGCAAA
    GGCCAGCCTC TCCCACCATT GCAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88691
    Clone ID Related Accession (Same CDS sequence) XM_024985555.1 , XM_015460654.1
    Accession Version XM_015460654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTTACTC TGTACAATGT GACCCACGTC TTGTCTTCGG GAAAGGAGCC TGTGATTGCC 
    CAGAGACAGA AGCACTCTGG GGCTGTCAGA GCCCTTGACT TTAATCCTTT CCAGGGCAAC
    CTCCTGGCCT CAGGGGCCAA TGATTCTGAA ATCTTCATTT GGGATTTGAA TAACCTGAGT
    GTACCAATGA CCCCAGGATC CAAGTCACAG CAGCCCCTGG AAGATATCAA GGCTCTCTCT
    TGGAACCGTC AAGTTCAACA CATTCTGTCT TCTGCTCACC CCAGCGGCAA GGCAGTCGTG
    TGGGATCTCA GGAAGAATGA ACCTATCATC AAAGTCAGTG ATCACAGCAA CCGGATGCAC
    TGCTCAGGCC TGGCCTGGCA CCCAGACATC GCCACCCAGT TGGTGCTTTG TTCAGAAGAT
    GATCGTCTTC CAGTAATTCA GCTGTGGGAC TTGCGCTTTG CCTCCTCACC CCTAAAGGTG
    CTGGAGAGTC ACAGCAGGGG GATCCTGTCA GTGTCATGGA GTCAGGCTGA TGCTGAGCTG
    CTGCTCAGTA GTGCCAAGGA CAGCCAGATC TTGTGCTGGA ACCTGGGGAG CAGTGAGGTG
    GTGTATAAAC TACCCATGCT GAGCAGCTGG TGCTTTGATG TGCAATGGTG CCCTCGGGAC
    CCTCTGGTGT TCTCTGCTGC TTCCTTTGAT GGCTGGATCA ATTTATACTC TGTGATGGGT
    AGGAGCTGGG AAGTCCAGCA GATGAGACAG GCTGACAAGA TCTCATCTTC CTTCAGCAAA
    GGCCAGCCTC TCCCACCATT GCAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316140.1
    CDS170..3385
    Translation

    Target ORF information:

    RefSeq Version XM_015460654.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTTACTC TGTACAATGT GACCCACGTC TTGTCTTCGG GAAAGGAGCC TGTGATTGCC 
    CAGAGACAGA AGCACTCTGG GGCTGTCAGA GCCCTTGACT TTAATCCTTT CCAGGGCAAC
    CTCCTGGCCT CAGGGGCCAA TGATTCTGAA ATCTTCATTT GGGATTTGAA TAACCTGAGT
    GTACCAATGA CCCCAGGATC CAAGTCACAG CAGCCCCTGG AAGATATCAA GGCTCTCTCT
    TGGAACCGTC AAGTTCAACA CATTCTGTCT TCTGCTCACC CCAGCGGCAA GGCAGTCGTG
    TGGGATCTCA GGAAGAATGA ACCTATCATC AAAGTCAGTG ATCACAGCAA CCGGATGCAC
    TGCTCAGGCC TGGCCTGGCA CCCAGACATC GCCACCCAGT TGGTGCTTTG TTCAGAAGAT
    GATCGTCTTC CAGTAATTCA GCTGTGGGAC TTGCGCTTTG CCTCCTCACC CCTAAAGGTG
    CTGGAGAGTC ACAGCAGGGG GATCCTGTCA GTGTCATGGA GTCAGGCTGA TGCTGAGCTG
    CTGCTCAGTA GTGCCAAGGA CAGCCAGATC TTGTGCTGGA ACCTGGGGAG CAGTGAGGTG
    GTGTATAAAC TACCCATGCT GAGCAGCTGG TGCTTTGATG TGCAATGGTG CCCTCGGGAC
    CCTCTGGTGT TCTCTGCTGC TTCCTTTGAT GGCTGGATCA ATTTATACTC TGTGATGGGT
    AGGAGCTGGG AAGTCCAGCA GATGAGACAG GCTGACAAGA TCTCATCTTC CTTCAGCAAA
    GGCCAGCCTC TCCCACCATT GCAGGTACCA GAGCAAGTGG CCCAAGCATC ACTGATACCT
    CCCCTGAAAA AACCCCCCAA ATGGATGAGG AGGCCAGCAG GTGTTTCATT TGCTTTTGGG
    GGCAAGCTGG TGACCTTTGG CCTCCCCAGC ACCCCTGTCC ATCAGGTGCC ACAGCCTTGC
    CTCCGCCTAG TCTTTATCAG TCAGGTCACC ACAGAACCTG AACTCCTGGC ACAGTCAGCT
    GAGCTGCAGG AGGCCCTCGG ATCAGGAAAT CTCCTGAATT ACTGTCAGAA CAAGATCCAC
    CAAGCATCAC TGCAAAGTGA AAAGATGCTC TGGCAGTTTC TGAAGGTGAC TTTAGAGAAG
    GACGCCAGGA TGAAGTTCCT GAAGCTATTA GGATACAGTA AAGATGAACT TCAGAAGAAG
    GTGGCCACAT GGTTGAGAAG TGATTTGAGG CTGGGTGAGA TCCCTCAGTC CAAGGGAGAT
    GACCTCAACA GTAACAGACA ACAGGCCTTC TCCATACAGA CCTCCAAACA TACCACCTGG
    GAAGCCTCGG TCTCCTCAGA CTTCTTTGAT GAGCTGATCC CTCAAAACAT GACTCTGTGG
    GAGATCCCCA TCACAGAAGA CACGGAGGGA CTCCTGAGCC GGGCTCTCCT GCTTGGAGAA
    CTGGGCCCTG CCGTAGAGCT GTGTCTGAAG GAAGAGCGCT TTGCTGATGC CATCATCCTG
    GCCCAGGCTG GAGGCTCAGA TCTGCTGAAG CAAGCACAGG AGCGCTACTT GGCCAAGAAG
    AAAACCGGAA TCGCCCCGCT GCTAGCCTGC ATTATACAGA AGAATTGGAA AGACATGGTG
    CATGCCTGTA GCCTGAAGAA CTGGAGAGAG GCTCTGGCCT TGCTGCTGAC ATACTCAGAG
    CCAGAGAAAT TCCCCGAGCT CTGTGACAGG CTGGGAACTC GTATGGAGCA GGAGAGTGGC
    TGGGCACTCA CCTCTGAAGC CAGACTCTGT TATGTGTGCT CAGGGAGTGT GGAGCGGCTG
    GTGGAGTGCT GGGCAAAATG CCAGCCGGCT TCATCCCCCA TGGCTTTACA GGACCTGATG
    GAGAAAGTGA TGGTCCTTAA CAGGAGCTTG GAGCTACTGC GGGGTCCTGA TGGGGTGAGC
    CCAGGCCCTG CCACAACCTA CAGAATCACT CAGTATGCCA ACCTCCTGGC AGCCCAGGGC
    AGCCTGGCCA CTGCCATGAG CTACCTACCC AGTGACTGTG CTCAGCTACC AGTTCAGCAG
    CTGAGAGATC GACTTTTTCA TGCCCAGGGT TCTGGTGTCT TGGGCCAACA GTCTCCCCCT
    TTCCCTTTTC CCCGGGTTGT CGTGGGAGCT ACCCCCTACT CTCAAGAGAC ATCCTCTCCC
    AGACTGAAAT TCCAGTCTTC TCACCAGGTT CCAATTCTAC CTCCAAGGCC AAGGATTTTT
    ACACCTCAGT CATCACTAAT GATGCCCTTG ACACCTTCCC ATCCTAGCCC TTATCAGGGC
    TCCAGAATGC AGAATACAAG TGACTACAGG CCATCTGGGG TCCAGGAAGC ACAGCCTTTG
    CCTCTGGGCC CTGGGGTAAG GCCTGCTTTA TCTCAGCCTC AGCTGTTAGG AGGGCAAAGG
    GCACAAGCTC CTAACCCCGT GGGATTCCCT GGAACATGGC CTCTCCTGGC TCCACCCATG
    GCACCCCCAA ACATTATGCA GCCTGGCTCT GTCCCCCTGC CTAGGGCTCC TCCAGTGCAC
    CCTCTGTTTC CTGTGAGAGC ACCAGGCTTC AGCCCTATGA GCTCCCAGCC ACCAGCCCCT
    CCTGTCAGCT TCCCTGCGGC CCACCCTCCA GGAGGGCCAG GTACTCCATG CGCTGGTGTC
    CTCCCGACCA CTGGCATCTT GATTCCTCAC CCAGGACCTC AGGATTCCTT GAAAAATGCT
    CCAGTGCCCA AGGGAAACCT CCAGAGGAAA AAGTTGCCAG AGACATTTAT GCCCCCAGCA
    CCAATTACTG CTCCAGTTAT GTGCCTCACC CCTGAGCCTC GAGGGGTCCT TTCCTCACAA
    CCTGCTGTCC CCAGTCAATG TCAGCAGCCA CTGGACAAGA TCAACAGGAA GGAGCTTCCC
    CCTGAACACC AGTCTTTGAA GATCAGCTTT GAGGCACTTC TGCAGCGCTG CTCCCTATCT
    GCCACTGATT TAAAGACAAA ACGGAAGCTA GATGAAGCTG CGCAACGTCT AGAATGTCTG
    TATGACAAGC TCTGCGAGGG GACAGTAAGT ACACTCAGTG GTTCTCATCT GCCTCCTGCT
    TTTCCACCCT CACCCCTGGC CAAAGGGCAC GCAGTGTCCC TTCCCATAAG CCTCACTGAC
    CCTGGGTTCC CTACAGAATA TAGACATGTT GCCTGGACTG GGCTCCAGAA CTCCAGAAGG
    GAGTCATCGC TTCTCCCTTC CATGCCTCCA GGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88692
    Clone ID Related Accession (Same CDS sequence) XM_015460655.1 , XM_015460655.2
    Accession Version XM_015460655.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGT TGCCAGAGAC ATTTATGCCC CCAGCACCAA TTACTGCTCC AGTTATGTGC
    CTCACCCCTG AGCCTCGAGG GGTCCTTTCC TCACAACCTG CTGTCCCCAG TCAATGTCAG
    CAGCCACTGG ACAAGATCAA CAGGAAGGAG CTTCCCCCTG AACACCAGTC TTTGAAGATC
    AGCTTTGAGG CACTTCTGCA GCGCTGCTCC CTATCTGCCA CTGATTTAAA GACAAAACGG
    AAGCTAGATG AAGCTGCGCA ACGTCTAGAA TGTCTGTATG ACAAGCTCTG CGAGGGGACA
    GTAAGTACAC TCAGTGGTTC TCATCTGCCT CCTGCTTTTC CACCCTCACC CCTGGCCAAA
    GGGCACGCAG TGTCCCTTCC CATAAGCCTC ACTGACCCTG GGTTCCCTAC AGAATATAGA
    CATGTTGCCT GGACTGGGCT CCAGAACTCC AGAAGGGAGT CATCGCTTCT CCCTTCCATG
    CCTCCAGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316141.1
    CDS103..3294
    Translation

    Target ORF information:

    RefSeq Version XM_015460655.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGT TGCCAGAGAC ATTTATGCCC CCAGCACCAA TTACTGCTCC AGTTATGTGC
    CTCACCCCTG AGCCTCGAGG GGTCCTTTCC TCACAACCTG CTGTCCCCAG TCAATGTCAG
    CAGCCACTGG ACAAGATCAA CAGGAAGGAG CTTCCCCCTG AACACCAGTC TTTGAAGATC
    AGCTTTGAGG CACTTCTGCA GCGCTGCTCC CTATCTGCCA CTGATTTAAA GACAAAACGG
    AAGCTAGATG AAGCTGCGCA ACGTCTAGAA TGTCTGTATG ACAAGCTCTG CGAGGGGACA
    GTAAGTACAC TCAGTGGTTC TCATCTGCCT CCTGCTTTTC CACCCTCACC CCTGGCCAAA
    GGGCACGCAG TGTCCCTTCC CATAAGCCTC ACTGACCCTG GGTTCCCTAC AGAATATAGA
    CATGTTGCCT GGACTGGGCT CCAGAACTCC AGAAGGGAGT CATCGCTTCT CCCTTCCATG
    CCTCCAGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88692
    Clone ID Related Accession (Same CDS sequence) XM_015460655.1 , XM_015460655.2
    Accession Version XM_015460655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGT TGCCAGAGAC ATTTATGCCC CCAGCACCAA TTACTGCTCC AGTTATGTGC
    CTCACCCCTG AGCCTCGAGG GGTCCTTTCC TCACAACCTG CTGTCCCCAG TCAATGTCAG
    CAGCCACTGG ACAAGATCAA CAGGAAGGAG CTTCCCCCTG AACACCAGTC TTTGAAGATC
    AGCTTTGAGG CACTTCTGCA GCGCTGCTCC CTATCTGCCA CTGATTTAAA GACAAAACGG
    AAGCTAGATG AAGCTGCGCA ACGTCTAGAA TGTCTGTATG ACAAGCTCTG CGAGGGGACA
    GTAAGTACAC TCAGTGGTTC TCATCTGCCT CCTGCTTTTC CACCCTCACC CCTGGCCAAA
    GGGCACGCAG TGTCCCTTCC CATAAGCCTC ACTGACCCTG GGTTCCCTAC AGAATATAGA
    CATGTTGCCT GGACTGGGCT CCAGAACTCC AGAAGGGAGT CATCGCTTCT CCCTTCCATG
    CCTCCAGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316141.1
    CDS126..3317
    Translation

    Target ORF information:

    RefSeq Version XM_015460655.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGT TGCCAGAGAC ATTTATGCCC CCAGCACCAA TTACTGCTCC AGTTATGTGC
    CTCACCCCTG AGCCTCGAGG GGTCCTTTCC TCACAACCTG CTGTCCCCAG TCAATGTCAG
    CAGCCACTGG ACAAGATCAA CAGGAAGGAG CTTCCCCCTG AACACCAGTC TTTGAAGATC
    AGCTTTGAGG CACTTCTGCA GCGCTGCTCC CTATCTGCCA CTGATTTAAA GACAAAACGG
    AAGCTAGATG AAGCTGCGCA ACGTCTAGAA TGTCTGTATG ACAAGCTCTG CGAGGGGACA
    GTAAGTACAC TCAGTGGTTC TCATCTGCCT CCTGCTTTTC CACCCTCACC CCTGGCCAAA
    GGGCACGCAG TGTCCCTTCC CATAAGCCTC ACTGACCCTG GGTTCCCTAC AGAATATAGA
    CATGTTGCCT GGACTGGGCT CCAGAACTCC AGAAGGGAGT CATCGCTTCT CCCTTCCATG
    CCTCCAGGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88693
    Clone ID Related Accession (Same CDS sequence) XM_015460656.1 , XM_015460656.2
    Accession Version XM_015460656.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGC AATGTCAGCA GCCACTGGAC AAGATCAACA GGAAGGAGCT TCCCCCTGAA
    CACCAGTCTT TGAAGATCAG CTTTGAGGCA CTTCTGCAGC GCTGCTCCCT ATCTGCCACT
    GATTTAAAGA CAAAACGGAA GCTAGATGAA GCTGCGCAAC GTCTAGAATG TCTGTATGAC
    AAGCTCTGCG AGGGGACAGT AAGTACACTC AGTGGTTCTC ATCTGCCTCC TGCTTTTCCA
    CCCTCACCCC TGGCCAAAGG GCACGCAGTG TCCCTTCCCA TAAGCCTCAC TGACCCTGGG
    TTCCCTACAG AATATAGACA TGTTGCCTGG ACTGGGCTCC AGAACTCCAG AAGGGAGTCA
    TCGCTTCTCC CTTCCATGCC TCCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316142.1
    CDS103..3192
    Translation

    Target ORF information:

    RefSeq Version XM_015460656.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGC AATGTCAGCA GCCACTGGAC AAGATCAACA GGAAGGAGCT TCCCCCTGAA
    CACCAGTCTT TGAAGATCAG CTTTGAGGCA CTTCTGCAGC GCTGCTCCCT ATCTGCCACT
    GATTTAAAGA CAAAACGGAA GCTAGATGAA GCTGCGCAAC GTCTAGAATG TCTGTATGAC
    AAGCTCTGCG AGGGGACAGT AAGTACACTC AGTGGTTCTC ATCTGCCTCC TGCTTTTCCA
    CCCTCACCCC TGGCCAAAGG GCACGCAGTG TCCCTTCCCA TAAGCCTCAC TGACCCTGGG
    TTCCCTACAG AATATAGACA TGTTGCCTGG ACTGGGCTCC AGAACTCCAG AAGGGAGTCA
    TCGCTTCTCC CTTCCATGCC TCCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88693
    Clone ID Related Accession (Same CDS sequence) XM_015460656.1 , XM_015460656.2
    Accession Version XM_015460656.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGC AATGTCAGCA GCCACTGGAC AAGATCAACA GGAAGGAGCT TCCCCCTGAA
    CACCAGTCTT TGAAGATCAG CTTTGAGGCA CTTCTGCAGC GCTGCTCCCT ATCTGCCACT
    GATTTAAAGA CAAAACGGAA GCTAGATGAA GCTGCGCAAC GTCTAGAATG TCTGTATGAC
    AAGCTCTGCG AGGGGACAGT AAGTACACTC AGTGGTTCTC ATCTGCCTCC TGCTTTTCCA
    CCCTCACCCC TGGCCAAAGG GCACGCAGTG TCCCTTCCCA TAAGCCTCAC TGACCCTGGG
    TTCCCTACAG AATATAGACA TGTTGCCTGG ACTGGGCTCC AGAACTCCAG AAGGGAGTCA
    TCGCTTCTCC CTTCCATGCC TCCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316142.1
    CDS126..3215
    Translation

    Target ORF information:

    RefSeq Version XM_015460656.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460656.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG GACCTCAGGA TTCCTTGAAA AATGCTCCAG TGCCCAAGGG AAACCTCCAG
    AGGAAAAAGC AATGTCAGCA GCCACTGGAC AAGATCAACA GGAAGGAGCT TCCCCCTGAA
    CACCAGTCTT TGAAGATCAG CTTTGAGGCA CTTCTGCAGC GCTGCTCCCT ATCTGCCACT
    GATTTAAAGA CAAAACGGAA GCTAGATGAA GCTGCGCAAC GTCTAGAATG TCTGTATGAC
    AAGCTCTGCG AGGGGACAGT AAGTACACTC AGTGGTTCTC ATCTGCCTCC TGCTTTTCCA
    CCCTCACCCC TGGCCAAAGG GCACGCAGTG TCCCTTCCCA TAAGCCTCAC TGACCCTGGG
    TTCCCTACAG AATATAGACA TGTTGCCTGG ACTGGGCTCC AGAACTCCAG AAGGGAGTCA
    TCGCTTCTCC CTTCCATGCC TCCAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33529
    Clone ID Related Accession (Same CDS sequence) NM_001192161.1 , NM_001192161.2
    Accession Version NM_001192161.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02058970.1. On Jul 17, 2010 this sequence version replaced XM_584571.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403652, SAMN02403653 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    GTGAGTCATG CTCCCCCCGG AGCCCCACGA GAACTCAGCC GGCAGATGAC AGGAATGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACACTCTCG
    CCTCCTGTGC TGGCCGGGCT CCATGAGATT GCCCGATGTG TGGACGCAGG AAGCCCTGAG
    CAGGGCCTTG CGATGCATGC CCAGGTGGTG GGCTGCAGCA GCTTCAGTGA GGTCTCCAGC
    TTCATGCCTG TCCTGAAGTC TGTCCTCACC ATTGCTCATA AGCTGCGCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179090.1
    CDS90..3623
    Translation

    Target ORF information:

    RefSeq Version NM_001192161.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    GTGAGTCATG CTCCCCCCGG AGCCCCACGA GAACTCAGCC GGCAGATGAC AGGAATGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACACTCTCG
    CCTCCTGTGC TGGCCGGGCT CCATGAGATT GCCCGATGTG TGGACGCAGG AAGCCCTGAG
    CAGGGCCTTG CGATGCATGC CCAGGTGGTG GGCTGCAGCA GCTTCAGTGA GGTCTCCAGC
    TTCATGCCTG TCCTGAAGTC TGTCCTCACC ATTGCTCATA AGCTGCGCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33529
    Clone ID Related Accession (Same CDS sequence) NM_001192161.1 , NM_001192161.2
    Accession Version NM_001192161.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000026.1. On May 29, 2018 this sequence version replaced NM_001192161.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    GTGAGTCATG CTCCCCCCGG AGCCCCACGA GAACTCAGCC GGCAGATGAC AGGAATGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACACTCTCG
    CCTCCTGTGC TGGCCGGGCT CCATGAGATT GCCCGATGTG TGGACGCAGG AAGCCCTGAG
    CAGGGCCTTG CGATGCATGC CCAGGTGGTG GGCTGCAGCA GCTTCAGTGA GGTCTCCAGC
    TTCATGCCTG TCCTGAAGTC TGTCCTCACC ATTGCTCATA AGCTGCGCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179090.1
    CDS90..3623
    Translation

    Target ORF information:

    RefSeq Version NM_001192161.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192161.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    GTGAGTCATG CTCCCCCCGG AGCCCCACGA GAACTCAGCC GGCAGATGAC AGGAATGCCA
    CTGGACAAGA TCAACAGGAA GGAGCTTCCC CCTGAACACC AGTCTTTGAA GATCAGCTTT
    GAGGCACTTC TGCAGCGCTG CTCCCTATCT GCCACTGATT TAAAGACAAA ACGGAAGCTA
    GATGAAGCTG CGCAACGTCT AGAATGTCTG TATGACAAGC TCTGCGAGGG GACACTCTCG
    CCTCCTGTGC TGGCCGGGCT CCATGAGATT GCCCGATGTG TGGACGCAGG AAGCCCTGAG
    CAGGGCCTTG CGATGCATGC CCAGGTGGTG GGCTGCAGCA GCTTCAGTGA GGTCTCCAGC
    TTCATGCCTG TCCTGAAGTC TGTCCTCACC ATTGCTCATA AGCTGCGCGT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46979
    Clone ID Related Accession (Same CDS sequence) XM_015460648.1 , XM_010819848.3 , XM_010819848.2
    Accession Version XM_015460648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAG TAAGTACACT CAGTGGTTCT CATCTGCCTC CTGCTTTTCC ACCCTCACCC
    CTGGCCAAAG GGCACGCAGT GTCCCTTCCC ATAAGCCTCA CTGACCCTGG GTTCCCTACA
    GAATATAGAC ATGTTGCCTG GACTGGGCTC CAGAACTCCA GAAGGGAGTC ATCGCTTCTC
    CCTTCCATGC CTCCAGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316134.1
    CDS103..3603
    Translation

    Target ORF information:

    RefSeq Version XM_015460648.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAG TAAGTACACT CAGTGGTTCT CATCTGCCTC CTGCTTTTCC ACCCTCACCC
    CTGGCCAAAG GGCACGCAGT GTCCCTTCCC ATAAGCCTCA CTGACCCTGG GTTCCCTACA
    GAATATAGAC ATGTTGCCTG GACTGGGCTC CAGAACTCCA GAAGGGAGTC ATCGCTTCTC
    CCTTCCATGC CTCCAGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46979
    Clone ID Related Accession (Same CDS sequence) XM_015460648.1 , XM_010819848.3 , XM_010819848.2
    Accession Version XM_010819848.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAG TAAGTACACT CAGTGGTTCT CATCTGCCTC CTGCTTTTCC ACCCTCACCC
    CTGGCCAAAG GGCACGCAGT GTCCCTTCCC ATAAGCCTCA CTGACCCTGG GTTCCCTACA
    GAATATAGAC ATGTTGCCTG GACTGGGCTC CAGAACTCCA GAAGGGAGTC ATCGCTTCTC
    CCTTCCATGC CTCCAGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818150.1
    CDS126..3626
    Translation

    Target ORF information:

    RefSeq Version XM_010819848.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819848.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAG TAAGTACACT CAGTGGTTCT CATCTGCCTC CTGCTTTTCC ACCCTCACCC
    CTGGCCAAAG GGCACGCAGT GTCCCTTCCC ATAAGCCTCA CTGACCCTGG GTTCCCTACA
    GAATATAGAC ATGTTGCCTG GACTGGGCTC CAGAACTCCA GAAGGGAGTC ATCGCTTCTC
    CCTTCCATGC CTCCAGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46979
    Clone ID Related Accession (Same CDS sequence) XM_015460648.1 , XM_010819848.3 , XM_010819848.2
    Accession Version XM_010819848.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAG TAAGTACACT CAGTGGTTCT CATCTGCCTC CTGCTTTTCC ACCCTCACCC
    CTGGCCAAAG GGCACGCAGT GTCCCTTCCC ATAAGCCTCA CTGACCCTGG GTTCCCTACA
    GAATATAGAC ATGTTGCCTG GACTGGGCTC CAGAACTCCA GAAGGGAGTC ATCGCTTCTC
    CCTTCCATGC CTCCAGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818150.1
    CDS103..3603
    Translation

    Target ORF information:

    RefSeq Version XM_010819848.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAG TAAGTACACT CAGTGGTTCT CATCTGCCTC CTGCTTTTCC ACCCTCACCC
    CTGGCCAAAG GGCACGCAGT GTCCCTTCCC ATAAGCCTCA CTGACCCTGG GTTCCCTACA
    GAATATAGAC ATGTTGCCTG GACTGGGCTC CAGAACTCCA GAAGGGAGTC ATCGCTTCTC
    CCTTCCATGC CTCCAGGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46981
    Clone ID Related Accession (Same CDS sequence) XM_010819850.2 , XM_010819850.3
    Accession Version XM_010819850.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACAGTAA GTACACTCAG TGGTTCTCAT CTGCCTCCTG CTTTTCCACC CTCACCCCTG
    GCCAAAGGGC ACGCAGTGTC CCTTCCCATA AGCCTCACTG ACCCTGGGTT CCCTACAGAA
    TATAGACATG TTGCCTGGAC TGGGCTCCAG AACTCCAGAA GGGAGTCATC GCTTCTCCCT
    TCCATGCCTC CAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818152.1
    CDS103..3600
    Translation

    Target ORF information:

    RefSeq Version XM_010819850.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACAGTAA GTACACTCAG TGGTTCTCAT CTGCCTCCTG CTTTTCCACC CTCACCCCTG
    GCCAAAGGGC ACGCAGTGTC CCTTCCCATA AGCCTCACTG ACCCTGGGTT CCCTACAGAA
    TATAGACATG TTGCCTGGAC TGGGCTCCAG AACTCCAGAA GGGAGTCATC GCTTCTCCCT
    TCCATGCCTC CAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46981
    Clone ID Related Accession (Same CDS sequence) XM_010819850.2 , XM_010819850.3
    Accession Version XM_010819850.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACAGTAA GTACACTCAG TGGTTCTCAT CTGCCTCCTG CTTTTCCACC CTCACCCCTG
    GCCAAAGGGC ACGCAGTGTC CCTTCCCATA AGCCTCACTG ACCCTGGGTT CCCTACAGAA
    TATAGACATG TTGCCTGGAC TGGGCTCCAG AACTCCAGAA GGGAGTCATC GCTTCTCCCT
    TCCATGCCTC CAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818152.1
    CDS126..3623
    Translation

    Target ORF information:

    RefSeq Version XM_010819850.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819850.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACAGTAA GTACACTCAG TGGTTCTCAT CTGCCTCCTG CTTTTCCACC CTCACCCCTG
    GCCAAAGGGC ACGCAGTGTC CCTTCCCATA AGCCTCACTG ACCCTGGGTT CCCTACAGAA
    TATAGACATG TTGCCTGGAC TGGGCTCCAG AACTCCAGAA GGGAGTCATC GCTTCTCCCT
    TCCATGCCTC CAGGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46990
    Clone ID Related Accession (Same CDS sequence) XM_010819860.2 , XM_010819860.3
    Accession Version XM_010819860.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAC TCTCGCCTCC TGTGCTGGCC GGGCTCCATG AGATTGCCCG ATGTGTGGAC
    GCAGGAAGCC CTGAGCAGGG CCTTGCGATG CATGCCCAGG TGGTGGGCTG CAGCAGCTTC
    AGTGAGGTCT CCAGCTTCAT GCCTGTCCTG AAGTCTGTCC TCACCATTGC TCATAAGCTG
    CGCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818162.1
    CDS103..3591
    Translation

    Target ORF information:

    RefSeq Version XM_010819860.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAC TCTCGCCTCC TGTGCTGGCC GGGCTCCATG AGATTGCCCG ATGTGTGGAC
    GCAGGAAGCC CTGAGCAGGG CCTTGCGATG CATGCCCAGG TGGTGGGCTG CAGCAGCTTC
    AGTGAGGTCT CCAGCTTCAT GCCTGTCCTG AAGTCTGTCC TCACCATTGC TCATAAGCTG
    CGCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46990
    Clone ID Related Accession (Same CDS sequence) XM_010819860.2 , XM_010819860.3
    Accession Version XM_010819860.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAC TCTCGCCTCC TGTGCTGGCC GGGCTCCATG AGATTGCCCG ATGTGTGGAC
    GCAGGAAGCC CTGAGCAGGG CCTTGCGATG CATGCCCAGG TGGTGGGCTG CAGCAGCTTC
    AGTGAGGTCT CCAGCTTCAT GCCTGTCCTG AAGTCTGTCC TCACCATTGC TCATAAGCTG
    CGCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818162.1
    CDS126..3614
    Translation

    Target ORF information:

    RefSeq Version XM_010819860.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819860.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCAGCC CCTGGAAGAT ATCAAGGCTC TCTCTTGGAA CCGTCAAGTT
    CAACACATTC TGTCTTCTGC TCACCCCAGC GGCAAGGCAG TCGTGTGGGA TCTCAGGAAG
    AATGAACCTA TCATCAAAGT CAGTGATCAC AGCAACCGGA TGCACTGCTC AGGCCTGGCC
    TGGCACCCAG ACATCGCCAC CCAGTTGGTG CTTTGTTCAG AAGATGATCG TCTTCCAGTA
    ATTCAGCTGT GGGACTTGCG CTTTGCCTCC TCACCCCTAA AGGTGCTGGA GAGTCACAGC
    AGGGGGATCC TGTCAGTGTC ATGGAGTCAG GCTGATGCTG AGCTGCTGCT CAGTAGTGCC
    AAGGACAGCC AGATCTTGTG CTGGAACCTG GGGAGCAGTG AGGTGGTGTA TAAACTACCC
    ATGCTGAGCA GCTGGTGCTT TGATGTGCAA TGGTGCCCTC GGGACCCTCT GGTGTTCTCT
    GCTGCTTCCT TTGATGGCTG GATCAATTTA TACTCTGTGA TGGGTAGGAG CTGGGAAGTC
    CAGCAGATGA GACAGGCTGA CAAGATCTCA TCTTCCTTCA GCAAAGGCCA GCCTCTCCCA
    CCATTGCAGG TACCAGAGCA AGTGGCCCAA GCATCACTGA TACCTCCCCT GAAAAAACCC
    CCCAAATGGA TGAGGAGGCC AGCAGGTGTT TCATTTGCTT TTGGGGGCAA GCTGGTGACC
    TTTGGCCTCC CCAGCACCCC TGTCCATCAG GTGCCACAGC CTTGCCTCCG CCTAGTCTTT
    ATCAGTCAGG TCACCACAGA ACCTGAACTC CTGGCACAGT CAGCTGAGCT GCAGGAGGCC
    CTCGGATCAG GAAATCTCCT GAATTACTGT CAGAACAAGA TCCACCAAGC ATCACTGCAA
    AGTGAAAAGA TGCTCTGGCA GTTTCTGAAG GTGACTTTAG AGAAGGACGC CAGGATGAAG
    TTCCTGAAGC TATTAGGATA CAGTAAAGAT GAACTTCAGA AGAAGGTGGC CACATGGTTG
    AGAAGTGATT TGAGGCTGGG TGAGATCCCT CAGTCCAAGG GAGATGACCT CAACAGTAAC
    AGACAACAGG CCTTCTCCAT ACAGACCTCC AAACATACCA CCTGGGAAGC CTCGGTCTCC
    TCAGACTTCT TTGATGAGCT GATCCCTCAA AACATGACTC TGTGGGAGAT CCCCATCACA
    GAAGACACGG AGGGACTCCT GAGCCGGGCT CTCCTGCTTG GAGAACTGGG CCCTGCCGTA
    GAGCTGTGTC TGAAGGAAGA GCGCTTTGCT GATGCCATCA TCCTGGCCCA GGCTGGAGGC
    TCAGATCTGC TGAAGCAAGC ACAGGAGCGC TACTTGGCCA AGAAGAAAAC CGGAATCGCC
    CCGCTGCTAG CCTGCATTAT ACAGAAGAAT TGGAAAGACA TGGTGCATGC CTGTAGCCTG
    AAGAACTGGA GAGAGGCTCT GGCCTTGCTG CTGACATACT CAGAGCCAGA GAAATTCCCC
    GAGCTCTGTG ACAGGCTGGG AACTCGTATG GAGCAGGAGA GTGGCTGGGC ACTCACCTCT
    GAAGCCAGAC TCTGTTATGT GTGCTCAGGG AGTGTGGAGC GGCTGGTGGA GTGCTGGGCA
    AAATGCCAGC CGGCTTCATC CCCCATGGCT TTACAGGACC TGATGGAGAA AGTGATGGTC
    CTTAACAGGA GCTTGGAGCT ACTGCGGGGT CCTGATGGGG TGAGCCCAGG CCCTGCCACA
    ACCTACAGAA TCACTCAGTA TGCCAACCTC CTGGCAGCCC AGGGCAGCCT GGCCACTGCC
    ATGAGCTACC TACCCAGTGA CTGTGCTCAG CTACCAGTTC AGCAGCTGAG AGATCGACTT
    TTTCATGCCC AGGGTTCTGG TGTCTTGGGC CAACAGTCTC CCCCTTTCCC TTTTCCCCGG
    GTTGTCGTGG GAGCTACCCC CTACTCTCAA GAGACATCCT CTCCCAGACT GAAATTCCAG
    TCTTCTCACC AGGTTCCAAT TCTACCTCCA AGGCCAAGGA TTTTTACACC TCAGTCATCA
    CTAATGATGC CCTTGACACC TTCCCATCCT AGCCCTTATC AGGGCTCCAG AATGCAGAAT
    ACAAGTGACT ACAGGCCATC TGGGGTCCAG GAAGCACAGC CTTTGCCTCT GGGCCCTGGG
    GTAAGGCCTG CTTTATCTCA GCCTCAGCTG TTAGGAGGGC AAAGGGCACA AGCTCCTAAC
    CCCGTGGGAT TCCCTGGAAC ATGGCCTCTC CTGGCTCCAC CCATGGCACC CCCAAACATT
    ATGCAGCCTG GCTCTGTCCC CCTGCCTAGG GCTCCTCCAG TGCACCCTCT GTTTCCTGTG
    AGAGCACCAG GCTTCAGCCC TATGAGCTCC CAGCCACCAG CCCCTCCTGT CAGCTTCCCT
    GCGGCCCACC CTCCAGGAGG GCCAGGTACT CCATGCGCTG GTGTCCTCCC GACCACTGGC
    ATCTTGATTC CTCACCCAGG ACCTCAGGAT TCCTTGAAAA ATGCTCCAGT GCCCAAGGGA
    AACCTCCAGA GGAAAAAGTT GCCAGAGACA TTTATGCCCC CAGCACCAAT TACTGCTCCA
    GTTATGTGCC TCACCCCTGA GCCTCGAGGG GTCCTTTCCT CACAACCTGC TGTCCCCAGT
    CAATGTCAGC AGCCACTGGA CAAGATCAAC AGGAAGGAGC TTCCCCCTGA ACACCAGTCT
    TTGAAGATCA GCTTTGAGGC ACTTCTGCAG CGCTGCTCCC TATCTGCCAC TGATTTAAAG
    ACAAAACGGA AGCTAGATGA AGCTGCGCAA CGTCTAGAAT GTCTGTATGA CAAGCTCTGC
    GAGGGGACAC TCTCGCCTCC TGTGCTGGCC GGGCTCCATG AGATTGCCCG ATGTGTGGAC
    GCAGGAAGCC CTGAGCAGGG CCTTGCGATG CATGCCCAGG TGGTGGGCTG CAGCAGCTTC
    AGTGAGGTCT CCAGCTTCAT GCCTGTCCTG AAGTCTGTCC TCACCATTGC TCATAAGCTG
    CGCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46991
    Clone ID Related Accession (Same CDS sequence) XM_010819861.3 , XM_010819861.2
    Accession Version XM_010819861.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACACTCT CGCCTCCTGT GCTGGCCGGG CTCCATGAGA TTGCCCGATG TGTGGACGCA
    GGAAGCCCTG AGCAGGGCCT TGCGATGCAT GCCCAGGTGG TGGGCTGCAG CAGCTTCAGT
    GAGGTCTCCA GCTTCATGCC TGTCCTGAAG TCTGTCCTCA CCATTGCTCA TAAGCTGCGC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818163.1
    CDS126..3611
    Translation

    Target ORF information:

    RefSeq Version XM_010819861.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819861.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACACTCT CGCCTCCTGT GCTGGCCGGG CTCCATGAGA TTGCCCGATG TGTGGACGCA
    GGAAGCCCTG AGCAGGGCCT TGCGATGCAT GCCCAGGTGG TGGGCTGCAG CAGCTTCAGT
    GAGGTCTCCA GCTTCATGCC TGTCCTGAAG TCTGTCCTCA CCATTGCTCA TAAGCTGCGC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46991
    Clone ID Related Accession (Same CDS sequence) XM_010819861.3 , XM_010819861.2
    Accession Version XM_010819861.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein transport protein Sec31B isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACACTCT CGCCTCCTGT GCTGGCCGGG CTCCATGAGA TTGCCCGATG TGTGGACGCA
    GGAAGCCCTG AGCAGGGCCT TGCGATGCAT GCCCAGGTGG TGGGCTGCAG CAGCTTCAGT
    GAGGTCTCCA GCTTCATGCC TGTCCTGAAG TCTGTCCTCA CCATTGCTCA TAAGCTGCGC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818163.1
    CDS103..3588
    Translation

    Target ORF information:

    RefSeq Version XM_010819861.2
    Organism Bos taurus(cattle)
    Definition Bos taurus SEC31 homolog B, COPII coat complex component (SEC31B), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819861.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGGAGCTTGA GCGACAAGCT GTCCAAGTGT GGAGCCCAGC CAGCCAGTAC 
    CCTGTGTATC TGGCCACAGG AACATCTGCC CAGCAGCTAG ATGCCTCCTT TAGTACAAAT
    GGCACATTGG AAATCTTTGA GGTTGATTTC AGGGACCCCT CTCTGGACTT GAAACGCAAG
    GGAGTCCTTT CTGCCTCCAG CAGGTTTCAC AAGCTGATCT GGGGGAGCTT TGGCAGTGGG
    CTTCTGGAAG CCTCCGGGGT CATTGCAGGC GGCGGGGACA ATGGCATGCT TACTCTGTAC
    AATGTGACCC ACGTCTTGTC TTCGGGAAAG GAGCCTGTGA TTGCCCAGAG ACAGAAGCAC
    TCTGGGGCTG TCAGAGCCCT TGACTTTAAT CCTTTCCAGG GCAACCTCCT GGCCTCAGGG
    GCCAATGATT CTGAAATCTT CATTTGGGAT TTGAATAACC TGAGTGTACC AATGACCCCA
    GGATCCAAGT CACAGCCCCT GGAAGATATC AAGGCTCTCT CTTGGAACCG TCAAGTTCAA
    CACATTCTGT CTTCTGCTCA CCCCAGCGGC AAGGCAGTCG TGTGGGATCT CAGGAAGAAT
    GAACCTATCA TCAAAGTCAG TGATCACAGC AACCGGATGC ACTGCTCAGG CCTGGCCTGG
    CACCCAGACA TCGCCACCCA GTTGGTGCTT TGTTCAGAAG ATGATCGTCT TCCAGTAATT
    CAGCTGTGGG ACTTGCGCTT TGCCTCCTCA CCCCTAAAGG TGCTGGAGAG TCACAGCAGG
    GGGATCCTGT CAGTGTCATG GAGTCAGGCT GATGCTGAGC TGCTGCTCAG TAGTGCCAAG
    GACAGCCAGA TCTTGTGCTG GAACCTGGGG AGCAGTGAGG TGGTGTATAA ACTACCCATG
    CTGAGCAGCT GGTGCTTTGA TGTGCAATGG TGCCCTCGGG ACCCTCTGGT GTTCTCTGCT
    GCTTCCTTTG ATGGCTGGAT CAATTTATAC TCTGTGATGG GTAGGAGCTG GGAAGTCCAG
    CAGATGAGAC AGGCTGACAA GATCTCATCT TCCTTCAGCA AAGGCCAGCC TCTCCCACCA
    TTGCAGGTAC CAGAGCAAGT GGCCCAAGCA TCACTGATAC CTCCCCTGAA AAAACCCCCC
    AAATGGATGA GGAGGCCAGC AGGTGTTTCA TTTGCTTTTG GGGGCAAGCT GGTGACCTTT
    GGCCTCCCCA GCACCCCTGT CCATCAGGTG CCACAGCCTT GCCTCCGCCT AGTCTTTATC
    AGTCAGGTCA CCACAGAACC TGAACTCCTG GCACAGTCAG CTGAGCTGCA GGAGGCCCTC
    GGATCAGGAA ATCTCCTGAA TTACTGTCAG AACAAGATCC ACCAAGCATC ACTGCAAAGT
    GAAAAGATGC TCTGGCAGTT TCTGAAGGTG ACTTTAGAGA AGGACGCCAG GATGAAGTTC
    CTGAAGCTAT TAGGATACAG TAAAGATGAA CTTCAGAAGA AGGTGGCCAC ATGGTTGAGA
    AGTGATTTGA GGCTGGGTGA GATCCCTCAG TCCAAGGGAG ATGACCTCAA CAGTAACAGA
    CAACAGGCCT TCTCCATACA GACCTCCAAA CATACCACCT GGGAAGCCTC GGTCTCCTCA
    GACTTCTTTG ATGAGCTGAT CCCTCAAAAC ATGACTCTGT GGGAGATCCC CATCACAGAA
    GACACGGAGG GACTCCTGAG CCGGGCTCTC CTGCTTGGAG AACTGGGCCC TGCCGTAGAG
    CTGTGTCTGA AGGAAGAGCG CTTTGCTGAT GCCATCATCC TGGCCCAGGC TGGAGGCTCA
    GATCTGCTGA AGCAAGCACA GGAGCGCTAC TTGGCCAAGA AGAAAACCGG AATCGCCCCG
    CTGCTAGCCT GCATTATACA GAAGAATTGG AAAGACATGG TGCATGCCTG TAGCCTGAAG
    AACTGGAGAG AGGCTCTGGC CTTGCTGCTG ACATACTCAG AGCCAGAGAA ATTCCCCGAG
    CTCTGTGACA GGCTGGGAAC TCGTATGGAG CAGGAGAGTG GCTGGGCACT CACCTCTGAA
    GCCAGACTCT GTTATGTGTG CTCAGGGAGT GTGGAGCGGC TGGTGGAGTG CTGGGCAAAA
    TGCCAGCCGG CTTCATCCCC CATGGCTTTA CAGGACCTGA TGGAGAAAGT GATGGTCCTT
    AACAGGAGCT TGGAGCTACT GCGGGGTCCT GATGGGGTGA GCCCAGGCCC TGCCACAACC
    TACAGAATCA CTCAGTATGC CAACCTCCTG GCAGCCCAGG GCAGCCTGGC CACTGCCATG
    AGCTACCTAC CCAGTGACTG TGCTCAGCTA CCAGTTCAGC AGCTGAGAGA TCGACTTTTT
    CATGCCCAGG GTTCTGGTGT CTTGGGCCAA CAGTCTCCCC CTTTCCCTTT TCCCCGGGTT
    GTCGTGGGAG CTACCCCCTA CTCTCAAGAG ACATCCTCTC CCAGACTGAA ATTCCAGTCT
    TCTCACCAGG TTCCAATTCT ACCTCCAAGG CCAAGGATTT TTACACCTCA GTCATCACTA
    ATGATGCCCT TGACACCTTC CCATCCTAGC CCTTATCAGG GCTCCAGAAT GCAGAATACA
    AGTGACTACA GGCCATCTGG GGTCCAGGAA GCACAGCCTT TGCCTCTGGG CCCTGGGGTA
    AGGCCTGCTT TATCTCAGCC TCAGCTGTTA GGAGGGCAAA GGGCACAAGC TCCTAACCCC
    GTGGGATTCC CTGGAACATG GCCTCTCCTG GCTCCACCCA TGGCACCCCC AAACATTATG
    CAGCCTGGCT CTGTCCCCCT GCCTAGGGCT CCTCCAGTGC ACCCTCTGTT TCCTGTGAGA
    GCACCAGGCT TCAGCCCTAT GAGCTCCCAG CCACCAGCCC CTCCTGTCAG CTTCCCTGCG
    GCCCACCCTC CAGGAGGGCC AGGTACTCCA TGCGCTGGTG TCCTCCCGAC CACTGGCATC
    TTGATTCCTC ACCCAGGACC TCAGGATTCC TTGAAAAATG CTCCAGTGCC CAAGGGAAAC
    CTCCAGAGGA AAAAGTTGCC AGAGACATTT ATGCCCCCAG CACCAATTAC TGCTCCAGTT
    ATGTGCCTCA CCCCTGAGCC TCGAGGGGTC CTTTCCTCAC AACCTGCTGT CCCCAGTCAA
    TGTCAGCAGC CACTGGACAA GATCAACAGG AAGGAGCTTC CCCCTGAACA CCAGTCTTTG
    AAGATCAGCT TTGAGGCACT TCTGCAGCGC TGCTCCCTAT CTGCCACTGA TTTAAAGACA
    AAACGGAAGC TAGATGAAGC TGCGCAACGT CTAGAATGTC TGTATGACAA GCTCTGCGAG
    GGGACACTCT CGCCTCCTGT GCTGGCCGGG CTCCATGAGA TTGCCCGATG TGTGGACGCA
    GGAAGCCCTG AGCAGGGCCT TGCGATGCAT GCCCAGGTGG TGGGCTGCAG CAGCTTCAGT
    GAGGTCTCCA GCTTCATGCC TGTCCTGAAG TCTGTCCTCA CCATTGCTCA TAAGCTGCGC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.