SPG11 cDNA ORF clone, Bos taurus(cattle)

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220006 XM_024998172.1
Latest version!
Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa06715 XM_002691092.5
Latest version!
Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa06715 XM_864766.6
Latest version!
Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa46952 XM_010809702.2
Latest version!
Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa46952 XM_015465259.1
Latest version!
Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa06715 XM_002691092.4 Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220006
    Clone ID Related Accession (Same CDS sequence) XM_024998172.1
    Accession Version XM_024998172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTTTTGGT TTCTGTCAGA ATCCGAGACA GCGCCAAAGG AACAGAAATC
    AGAGAGGATC GCTGCCATGC TCCAGGCTGT CGCACCCATT CACCCCAGGT GCGCCTCCTC
    ACTGGCATCG GAAGGTACAA TGAGATGACA TACATATTTG ATTTGCTGCA TAAAAAGCAC
    TACTTTGAAG TGCTCATGAG GAAGAAGCTG GACCCTAGTG GCACCCTGAA GACGGCCCTG
    CTGGACTACA TCAAGCGCTG CCGCCCTGGG GACAGCGAGA AGCACAACAT GATCGCGCTG
    TGCTTCAGCA TGTGCCGCGA GATCGGCGAA AACCACGAGG CAGCTGCCTG CATCCAACTG
    AAACTGATCG AGTCTCAGCC CTGGGAGGAC TGTGTCAAGG ATGGGCAGCG ACTGAAGCAG
    CTGCTGCTGA AGGCCCTGAC TCTGATGCTG GACGCGGCAG AGAGTTATGC CAAGGACTCC
    TGCGTGCGCC AGGCCCTGCA CTGTCACCGG CTCACCAAGT TGATAACGCT GCAGATTCAC
    TTTCTGAACA CTGGCCAAAA CACGATGCTC ATCAACCTGA GCCGCCACAG GCTGATGGAC
    TGTATCATGG CGCTGCCTCG GTTCTACCAG GCTTCCATTG TGGCAGAGGC CTATGACTTT
    GTTCCTGACT GGGCTGAAAT TCTCTATCAA CAAGTGATTC TTAAAGGAGA CTTTAATTAC
    TTGGAAGAAT TTAAGCAGCA AAGGTTATTA AGACCCAGTG TATTTGAAGA GATTTCAATA
    AAATGTAAAC ATCATCAGCC TACAGAATTT GTTCTGAAAA ACCTGAAGAA GTTACTCACA
    TATTGTGAAG ACATCTACTT GTATTACAAG CTGGCATACG AACACAAACT TTACGATGTT
    GTAAATGTGC TTCTGAAGGA CCCTCAGACC GGCTGCTGTC TAAAGGACAT GCTGGCCGGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853940.1
    CDS38..7420
    Translation

    Target ORF information:

    RefSeq Version XM_024998172.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTTTTGGT TTCTGTCAGA ATCCGAGACA GCGCCAAAGG AACAGAAATC
    AGAGAGGATC GCTGCCATGC TCCAGGCTGT CGCACCCATT CACCCCAGGT GCGCCTCCTC
    ACTGGCATCG GAAGGTACAA TGAGATGACA TACATATTTG ATTTGCTGCA TAAAAAGCAC
    TACTTTGAAG TGCTCATGAG GAAGAAGCTG GACCCTAGTG GCACCCTGAA GACGGCCCTG
    CTGGACTACA TCAAGCGCTG CCGCCCTGGG GACAGCGAGA AGCACAACAT GATCGCGCTG
    TGCTTCAGCA TGTGCCGCGA GATCGGCGAA AACCACGAGG CAGCTGCCTG CATCCAACTG
    AAACTGATCG AGTCTCAGCC CTGGGAGGAC TGTGTCAAGG ATGGGCAGCG ACTGAAGCAG
    CTGCTGCTGA AGGCCCTGAC TCTGATGCTG GACGCGGCAG AGAGTTATGC CAAGGACTCC
    TGCGTGCGCC AGGCCCTGCA CTGTCACCGG CTCACCAAGT TGATAACGCT GCAGATTCAC
    TTTCTGAACA CTGGCCAAAA CACGATGCTC ATCAACCTGA GCCGCCACAG GCTGATGGAC
    TGTATCATGG CGCTGCCTCG GTTCTACCAG GCTTCCATTG TGGCAGAGGC CTATGACTTT
    GTTCCTGACT GGGCTGAAAT TCTCTATCAA CAAGTGATTC TTAAAGGAGA CTTTAATTAC
    TTGGAAGAAT TTAAGCAGCA AAGGTTATTA AGACCCAGTG TATTTGAAGA GATTTCAATA
    AAATGTAAAC ATCATCAGCC TACAGAATTT GTTCTGAAAA ACCTGAAGAA GTTACTCACA
    TATTGTGAAG ACATCTACTT GTATTACAAG CTGGCATACG AACACAAACT TTACGATGTT
    GTAAATGTGC TTCTGAAGGA CCCTCAGACC GGCTGCTGTC TAAAGGACAT GCTGGCCGGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06715
    Clone ID Related Accession (Same CDS sequence) XM_002691092.5 , XM_864766.6 , XM_002691092.4
    Accession Version XM_002691092.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691092.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA
    TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT
    AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC
    GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC
    GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC
    AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG
    GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC
    AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC
    CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC
    ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG
    ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC
    AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG
    AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA
    TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC
    TGTCTAAAGG ACATGCTGGC CGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691138.2
    CDS19..7305
    Translation

    Target ORF information:

    RefSeq Version XM_002691092.5
    Organism Bos taurus(cattle)
    Definition Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691092.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA
    TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT
    AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC
    GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC
    GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC
    AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG
    GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC
    AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC
    CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC
    ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG
    ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC
    AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG
    AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA
    TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC
    TGTCTAAAGG ACATGCTGGC CGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06715
    Clone ID Related Accession (Same CDS sequence) XM_002691092.5 , XM_864766.6 , XM_002691092.4
    Accession Version XM_864766.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_864766.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA
    TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT
    AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC
    GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC
    GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC
    AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG
    GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC
    AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC
    CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC
    ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG
    ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC
    AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG
    AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA
    TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC
    TGTCTAAAGG ACATGCTGGC CGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_869859.3
    CDS49..7335
    Translation

    Target ORF information:

    RefSeq Version XM_864766.6
    Organism Bos taurus(cattle)
    Definition Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_864766.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA
    TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT
    AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC
    GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC
    GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC
    AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG
    GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC
    AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC
    CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC
    ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG
    ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC
    AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG
    AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA
    TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC
    TGTCTAAAGG ACATGCTGGC CGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06715
    Clone ID Related Accession (Same CDS sequence) XM_002691092.5 , XM_864766.6 , XM_002691092.4
    Accession Version XM_002691092.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spatacsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104285.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA
    TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT
    AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC
    GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC
    GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC
    AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG
    GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC
    AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC
    CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC
    ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG
    ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC
    AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG
    AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA
    TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC
    TGTCTAAAGG ACATGCTGGC CGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691138.2
    CDS49..7335
    Translation

    Target ORF information:

    RefSeq Version XM_002691092.4
    Organism Bos taurus(cattle)
    Definition Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691092.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA
    TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT
    AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC
    GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC
    GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC
    AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG
    GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC
    AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC
    CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC
    ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG
    ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC
    AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG
    AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA
    TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC
    TGTCTAAAGG ACATGCTGGC CGGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46952
    Clone ID Related Accession (Same CDS sequence) XM_015465259.1 , XM_010809702.2
    Accession Version XM_015465259.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320745.1
    CDS51..6521
    Translation

    Target ORF information:

    RefSeq Version XM_015465259.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465259.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46952
    Clone ID Related Accession (Same CDS sequence) XM_015465259.1 , XM_010809702.2
    Accession Version XM_010809702.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spatacsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808004.1
    CDS51..6521
    Translation

    Target ORF information:

    RefSeq Version XM_010809702.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809702.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
    GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG
    GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC
    CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA
    AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT
    AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG
    CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA
    TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT
    CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG
    GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT
    TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA
    GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA
    TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT
    ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT
    GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT
    GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG
    AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC
    TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT
    ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT
    CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC
    AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG
    TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG
    AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG
    CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA
    GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT
    CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA
    AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA
    TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA
    CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT
    TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT
    AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA
    GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC
    TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG
    GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC
    AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT
    GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG
    GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG
    ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA
    AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG
    TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA
    AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT
    AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT
    CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT
    TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG
    TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA
    CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA
    ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA
    CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG
    AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA
    CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT
    TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT
    CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT
    GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA
    GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT
    GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG
    GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT
    GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT
    AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG
    CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT
    TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG
    GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT
    GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA
    GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT
    TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT
    GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC
    TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA
    TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC
    ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC
    CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT
    CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG
    GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG
    ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC
    CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG
    GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT
    GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG
    GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA
    GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC
    TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC
    CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG
    AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG
    CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT
    GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT
    GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA
    AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA
    CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT
    GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT
    AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG
    GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC
    ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC
    CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG
    GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC
    TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA
    CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA
    TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA
    GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT
    CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA
    AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC
    ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG
    AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC
    TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT
    GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT
    CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA
    GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC
    CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG
    GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC
    ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC
    ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG
    TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.