SPG11 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPG11
Entrez Gene ID	507819
Full Name	SPG11, spatacsin vesicle trafficking associated
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names spatacsin spastic paraplegia 11 (autosomal recessive)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002691092.5	XP_002691138.2	spatacsin isoform X2
XM_864766.6	XP_869859.3	spatacsin isoform X1
XM_015465259.1	XP_015320745.1	spatacsin isoform X2
XM_010809702.2	XP_010808004.1	spatacsin isoform X2
XM_024998172.1	XP_024853940.1	spatacsin isoform X1
XM_002691092.4	XP_002691138.2	spatacsin isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Gene Ontology

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220006	XM_024998172.1 Latest version!	Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa06715	XM_002691092.5 Latest version!	Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa06715	XM_864766.6 Latest version!	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa46952	XM_010809702.2 Latest version!	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa46952	XM_015465259.1 Latest version!	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa06715	XM_002691092.4	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPG11 ( XM_024998172.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853940.1 Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

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CloneID	OBa220006
Clone ID Related Accession (Same CDS sequence)	XM_024998172.1
Accession Version	XM_024998172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTTTTGGT TTCTGTCAGA ATCCGAGACA GCGCCAAAGG AACAGAAATC 
AGAGAGGATC GCTGCCATGC TCCAGGCTGT CGCACCCATT CACCCCAGGT GCGCCTCCTC 
ACTGGCATCG GAAGGTACAA TGAGATGACA TACATATTTG ATTTGCTGCA TAAAAAGCAC 
TACTTTGAAG TGCTCATGAG GAAGAAGCTG GACCCTAGTG GCACCCTGAA GACGGCCCTG 
CTGGACTACA TCAAGCGCTG CCGCCCTGGG GACAGCGAGA AGCACAACAT GATCGCGCTG 
TGCTTCAGCA TGTGCCGCGA GATCGGCGAA AACCACGAGG CAGCTGCCTG CATCCAACTG 
AAACTGATCG AGTCTCAGCC CTGGGAGGAC TGTGTCAAGG ATGGGCAGCG ACTGAAGCAG 
CTGCTGCTGA AGGCCCTGAC TCTGATGCTG GACGCGGCAG AGAGTTATGC CAAGGACTCC 
TGCGTGCGCC AGGCCCTGCA CTGTCACCGG CTCACCAAGT TGATAACGCT GCAGATTCAC 
TTTCTGAACA CTGGCCAAAA CACGATGCTC ATCAACCTGA GCCGCCACAG GCTGATGGAC 
TGTATCATGG CGCTGCCTCG GTTCTACCAG GCTTCCATTG TGGCAGAGGC CTATGACTTT 
GTTCCTGACT GGGCTGAAAT TCTCTATCAA CAAGTGATTC TTAAAGGAGA CTTTAATTAC 
TTGGAAGAAT TTAAGCAGCA AAGGTTATTA AGACCCAGTG TATTTGAAGA GATTTCAATA 
AAATGTAAAC ATCATCAGCC TACAGAATTT GTTCTGAAAA ACCTGAAGAA GTTACTCACA 
TATTGTGAAG ACATCTACTT GTATTACAAG CTGGCATACG AACACAAACT TTACGATGTT 
GTAAATGTGC TTCTGAAGGA CCCTCAGACC GGCTGCTGTC TAAAGGACAT GCTGGCCGGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853940.1
CDS	38..7420
Translation	MAAEQGALPVLLVPVPAEAVGRLGSRAQLHREQAALGSLTAAGSLHVLPLAPGGRGGGDCRLEGCFRQFVWEDSHHSGTPNNKPRLLALTENYELLICEFNLEHGRCDATILYSYSEETLQKLVEDQNISISLLSLRILSFHNNTSLLFINKCVVLHVMFPERDAEIRVLSCCTLPLPAQAVDRIIDTQLCRGILFILSSLGWIYIFDIVDGKQVAHVDLALAQEDMCNEQQQESAKSSSFTSLKVSQDLDVVVVVGFSNTAVALNLTLYFRQYPGHLLCEKTLQDSPIEGPKGIDEDDPVNSDYNMKLTKFSFRIDRSWKAQLSSLNETIKSSKLGGSCCAPWFQDISHLESPESGNRSTSVSDWAFIPQDVTCSPCNFPQKGPARTSDPGRVWKRMHIREQEEPLELTCVSVTGFTALFTWAGESTGWTIVLWDLETQSTQCFSLGKKCIPVDSGGVQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSSVPDQFDHFASHLYLKHVEELTPALDLLCSAIRENDSETQSKHFSEQLLNLTLSFLNKQIKELFIHIEELDEHLQKGVDILTSYIDELRTFMIKFPWKLTDAIDEYDVNENVPKVKESNIWEKLSVEEVIANAILNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNMEEASELLKNMGFNVKDQLLKICFYTADKSVRDFLVEIFKEKNYFSEKEKRTVDFVHQVEKFYSGHLQENIQIQSLPRYWIKEQDFSKHKSVLDSFLKYDHKDKLNKQDHRIVLNWAQWWNQPTQESILLPWINPEGYNSYSPEGLWRYLTARHDWPSIILWIGEFQTQSSYASLEQNKWPPLTVDVVDQNTCCNNYMRNEILDKLARNGIFLTSELEDFELFLVRLNRVGGVMQDALAVQNYRSTEGWGFHSHFILYCLEHSLQYLLYVYLDHYKLSPPNCPFLGKKELHEAHPWFEFLVQCRQVSSNLTDPRLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNGENENSLKKVDPQLLKMALTPYPKLKAALFPQYTAPSVLPPDITLYHLIQSLLPFDPSRLFGWQSANTLAIGDAASQLPHFSSPDLVNKYAVVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGHEAYVLGLSCFHISSIGAACVCFLELLGLDSLKLRVDMKVANIILNYKCGNEDAPYNSIRESLTEKLSKLALGERATIEELLVLLEEGIWSNIQQQQIQRLSSESSSQWALVVQFCRLHNIKPSTSYLRECARANDWLQFIIHSQLHNHHPEEVKYLLQYFSPVLQDHLRLAFENLPSVSNSRMDSDQVCNRSSQELQRHKEEMTDFFQILLQCSEVPDSWCWLLGEAVRQKAPILSVLASCLQDASALSCLCVWIIASVEPHVAAEAVGPLQDSAEGHVWGLEDLSVIWRTLLTRQKIRTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLVEFQQSLETLETAATRVPAAIPAPWLKEHVCFLLKLMLQQCSTQYELGRLLQLFVGIEHLFSDGPDVKKLCVLSQILKDTPIAINRAIITSYSLENFQRECRSVLEKLETDGHFALARRVAELAELPVDDLVIEEITQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSVSSRAASSFFSAQALWAGECPAAGGPSSAEERHLLLTLAGHWLAREDPVPLEDLEGLEKQIWLCRVAQHTLRGSQEEGGPRAPQQASAGGELSFDGLASEFSFSNLAVLNTSKYLELTGFPSRDICDNELNWKEQASLNCLIGRLLDDGCVHEASRACRYFRFYHQDVALVLHCRALASGEASVDDLHPEILALLRSTELPEEEEEAGIPLRKVQSTSSLDSWSLVMLPPADEVVTNLETLTSKCLRGKNYCRQVLCLYELAKELGCSYADVAAQDGEAVLRAILASQQPNRCKRAQAFISTQGLRPDTVAELVAEEVTRELLTPSEGTGHRQVFAPAEESQTFLQLTALCQDRTLVGMKLLEKISSVPHGELSCTTELLILAHHCFTLTCHMEGIMRVLQAARLLTDNHLAPSEEYGLVVLVSVRIRDSAKGTEIREDRCHAPGCRTHSPQVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDCVKDGQRLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRPSVFEEISIKCKHHQPTEFVLKNLKKLLTYCEDIYLYYKLAYEHKLYDVVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_024998172.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998172.1

ORF Insert Sequence:

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTTTTGGT TTCTGTCAGA ATCCGAGACA GCGCCAAAGG AACAGAAATC 
AGAGAGGATC GCTGCCATGC TCCAGGCTGT CGCACCCATT CACCCCAGGT GCGCCTCCTC 
ACTGGCATCG GAAGGTACAA TGAGATGACA TACATATTTG ATTTGCTGCA TAAAAAGCAC 
TACTTTGAAG TGCTCATGAG GAAGAAGCTG GACCCTAGTG GCACCCTGAA GACGGCCCTG 
CTGGACTACA TCAAGCGCTG CCGCCCTGGG GACAGCGAGA AGCACAACAT GATCGCGCTG 
TGCTTCAGCA TGTGCCGCGA GATCGGCGAA AACCACGAGG CAGCTGCCTG CATCCAACTG 
AAACTGATCG AGTCTCAGCC CTGGGAGGAC TGTGTCAAGG ATGGGCAGCG ACTGAAGCAG 
CTGCTGCTGA AGGCCCTGAC TCTGATGCTG GACGCGGCAG AGAGTTATGC CAAGGACTCC 
TGCGTGCGCC AGGCCCTGCA CTGTCACCGG CTCACCAAGT TGATAACGCT GCAGATTCAC 
TTTCTGAACA CTGGCCAAAA CACGATGCTC ATCAACCTGA GCCGCCACAG GCTGATGGAC 
TGTATCATGG CGCTGCCTCG GTTCTACCAG GCTTCCATTG TGGCAGAGGC CTATGACTTT 
GTTCCTGACT GGGCTGAAAT TCTCTATCAA CAAGTGATTC TTAAAGGAGA CTTTAATTAC 
TTGGAAGAAT TTAAGCAGCA AAGGTTATTA AGACCCAGTG TATTTGAAGA GATTTCAATA 
AAATGTAAAC ATCATCAGCC TACAGAATTT GTTCTGAAAA ACCTGAAGAA GTTACTCACA 
TATTGTGAAG ACATCTACTT GTATTACAAG CTGGCATACG AACACAAACT TTACGATGTT 
GTAAATGTGC TTCTGAAGGA CCCTCAGACC GGCTGCTGTC TAAAGGACAT GCTGGCCGGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_002691092.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691138.2 Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

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CloneID	OBa06715
Clone ID Related Accession (Same CDS sequence)	XM_002691092.5 , XM_864766.6 , XM_002691092.4
Accession Version	XM_002691092.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691092.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT 
AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC 
GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC 
GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC 
AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG 
GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC 
AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC 
ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC 
AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG 
AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA 
TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC 
TGTCTAAAGG ACATGCTGGC CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691138.2
CDS	19..7305
Translation	MAAEQGALPVLLVPVPAEAVGRLGSRAQLHREQAALGSLTAAGSLHVLPLAPGGRGGGDCRLEGCFRQFVWEDSHHSGTPNNKPRLLALTENYELLICEFNLEHGRCDATILYSYSEETLQKLVEDQNISISLLSLRILSFHNNTSLLFINKCVVLHVMFPERDAEIRVLSCCTLPLPAQAVDRIIDTQLCRGILFILSSLGWIYIFDIVDGKQVAHVDLALAQEDMCNEQQQESAKSSSFTSLKVSQDLDVVVVVGFSNTAVALNLTLYFRQYPGHLLCEKTLQDSPIEGPKGIDEDDPVNSDYNMKLTKFSFRIDRSWKAQLSSLNETIKSSKLGGSCCAPWFQDISHLESPESGNRSTSVSDWAFIPQDVTCSPCNFPQKGPARTSDPGRVWKRMHIREQEEPLELTCVSVTGFTALFTWAGESTGWTIVLWDLETQSTQCFSLGKKCIPVDSGGVQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSSVPDQFDHFASHLYLKHVEELTPALDLLCSAIRENDSETQSKHFSEQLLNLTLSFLNKQIKELFIHIEELDEHLQKGVDILTSYIDELRTFMIKFPWKLTDAIDEYDVNENVPKVKESNIWEKLSVEEVIANAILNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNMEEASELLKNMGFNVKDQLLKICFYTADKSVRDFLVEIFKEKNYFSEKEKRTVDFVHQVEKFYSGHLQENIQIQSLPRYWIKEQDFSKHKSVLDSFLKYDHKDKLNKQDHRIVLNWAQWWNQPTQESILLPWINPEGYNSYSPEGLWRYLTARHDWPSIILWIGEFQTQSSYASLEQNKWPPLTVDVVDQNTCCNNYMRNEILDKLARNGIFLTSELEDFELFLVRLNRVGGVMQDALAVQNYRSTEGWGFHSHFILYCLEHSLQYLLYVYLDHYKLSPPNCPFLGKKELHEAHPWFEFLVQCRQVSSNLTDPRLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNGENENSLKKVDPQLLKMALTPYPKLKAALFPQYTAPSVLPPDITLYHLIQSLLPFDPSRLFGWQSANTLAIGDAASQLPHFSSPDLVNKYAVVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGHEAYVLGLSCFHISSIGAACVCFLELLGLDSLKLRVDMKVANIILNYKCGNEDAPYNSIRESLTEKLSKLALGERATIEELLVLLEEGIWSNIQQQQIQRLSSESSSQWALVVQFCRLHNIKPSTSYLRECARANDWLQFIIHSQLHNHHPEEVKYLLQYFSPVLQDHLRLAFENLPSVSNSRMDSDQVCNRSSQELQRHKEEMTDFFQILLQCSEVPDSWCWLLGEAVRQKAPILSVLASCLQDASALSCLCVWIIASVEPHVAAEAVGPLQDSAEGHVWGLEDLSVIWRTLLTRQKIRTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLVEFQQSLETLETAATRVPAAIPAPWLKEHVCFLLKLMLQQCSTQYELGRLLQLFVGIEHLFSDGPDVKKLCVLSQILKDTPIAINRAIITSYSLENFQRECRSVLEKLETDGHFALARRVAELAELPVDDLVIEEITQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSVSSRAASSFFSAQALWAGECPAAGGPSSAEERHLLLTLAGHWLAREDPVPLEDLEGLEKQIWLCRVAQHTLRGSQEEGGPRAPQQASAGGELSFDGLASEFSFSNLAVLNTSKYLELTGFPSRDICDNELNWKEQASLNCLIGRLLDDGCVHEASRACRYFRFYHQDVALVLHCRALASGEASVDDLHPEILALLRSTELPEEEEEAGIPLRKVQSTSSLDSWSLVMLPPADEVVTNLETLTSKCLRGKNYCRQVLCLYELAKELGCSYADVAAQDGEAVLRAILASQQPNRCKRAQAFISTQGLRPDTVAELVAEEVTRELLTPSEGTGHRQVFAPAEESQTFLQLTALCQDRTLVGMKLLEKISSVPHGELSCTTELLILAHHCFTLTCHMEGIMRVLQAARLLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDCVKDGQRLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRPSVFEEISIKCKHHQPTEFVLKNLKKLLTYCEDIYLYYKLAYEHKLYDVVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_002691092.5
Organism	Bos taurus(cattle)
Definition	Bos taurus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691092.5

ORF Insert Sequence:

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT 
AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC 
GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC 
GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC 
AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG 
GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC 
AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC 
ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC 
AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG 
AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA 
TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC 
TGTCTAAAGG ACATGCTGGC CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_864766.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_869859.3 Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

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CloneID	OBa06715
Clone ID Related Accession (Same CDS sequence)	XM_002691092.5 , XM_864766.6 , XM_002691092.4
Accession Version	XM_864766.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_864766.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT 
AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC 
GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC 
GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC 
AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG 
GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC 
AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC 
ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC 
AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG 
AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA 
TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC 
TGTCTAAAGG ACATGCTGGC CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_869859.3
CDS	49..7335
Translation	MAAEQGALPVLLVPVPAEAVGRLGSRAQLHREQAALGSLTAAGSLHVLPLAPGGRGGGDCRLEGCFRQFVWEDSHHSGTPNNKPRLLALTENYELLICEFNLEHGRCDATILYSYSEETLQKLVEDQNISISLLSLRILSFHNNTSLLFINKCVVLHVMFPERDAEIRVLSCCTLPLPAQAVDRIIDTQLCRGILFILSSLGWIYIFDIVDGKQVAHVDLALAQEDMCNEQQQESAKSSSFTSLKVSQDLDVVVVVGFSNTAVALNLTLYFRQYPGHLLCEKTLQDSPIEGPKGIDEDDPVNSDYNMKLTKFSFRIDRSWKAQLSSLNETIKSSKLGGSCCAPWFQDISHLESPESGNRSTSVSDWAFIPQDVTCSPCNFPQKGPARTSDPGRVWKRMHIREQEEPLELTCVSVTGFTALFTWAGESTGWTIVLWDLETQSTQCFSLGKKCIPVDSGGVQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSSVPDQFDHFASHLYLKHVEELTPALDLLCSAIRENDSETQSKHFSEQLLNLTLSFLNKQIKELFIHIEELDEHLQKGVDILTSYIDELRTFMIKFPWKLTDAIDEYDVNENVPKVKESNIWEKLSVEEVIANAILNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNMEEASELLKNMGFNVKDQLLKICFYTADKSVRDFLVEIFKEKNYFSEKEKRTVDFVHQVEKFYSGHLQENIQIQSLPRYWIKEQDFSKHKSVLDSFLKYDHKDKLNKQDHRIVLNWAQWWNQPTQESILLPWINPEGYNSYSPEGLWRYLTARHDWPSIILWIGEFQTQSSYASLEQNKWPPLTVDVVDQNTCCNNYMRNEILDKLARNGIFLTSELEDFELFLVRLNRVGGVMQDALAVQNYRSTEGWGFHSHFILYCLEHSLQYLLYVYLDHYKLSPPNCPFLGKKELHEAHPWFEFLVQCRQVSSNLTDPRLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNGENENSLKKVDPQLLKMALTPYPKLKAALFPQYTAPSVLPPDITLYHLIQSLLPFDPSRLFGWQSANTLAIGDAASQLPHFSSPDLVNKYAVVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGHEAYVLGLSCFHISSIGAACVCFLELLGLDSLKLRVDMKVANIILNYKCGNEDAPYNSIRESLTEKLSKLALGERATIEELLVLLEEGIWSNIQQQQIQRLSSESSSQWALVVQFCRLHNIKPSTSYLRECARANDWLQFIIHSQLHNHHPEEVKYLLQYFSPVLQDHLRLAFENLPSVSNSRMDSDQVCNRSSQELQRHKEEMTDFFQILLQCSEVPDSWCWLLGEAVRQKAPILSVLASCLQDASALSCLCVWIIASVEPHVAAEAVGPLQDSAEGHVWGLEDLSVIWRTLLTRQKIRTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLVEFQQSLETLETAATRVPAAIPAPWLKEHVCFLLKLMLQQCSTQYELGRLLQLFVGIEHLFSDGPDVKKLCVLSQILKDTPIAINRAIITSYSLENFQRECRSVLEKLETDGHFALARRVAELAELPVDDLVIEEITQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSVSSRAASSFFSAQALWAGECPAAGGPSSAEERHLLLTLAGHWLAREDPVPLEDLEGLEKQIWLCRVAQHTLRGSQEEGGPRAPQQASAGGELSFDGLASEFSFSNLAVLNTSKYLELTGFPSRDICDNELNWKEQASLNCLIGRLLDDGCVHEASRACRYFRFYHQDVALVLHCRALASGEASVDDLHPEILALLRSTELPEEEEEAGIPLRKVQSTSSLDSWSLVMLPPADEVVTNLETLTSKCLRGKNYCRQVLCLYELAKELGCSYADVAAQDGEAVLRAILASQQPNRCKRAQAFISTQGLRPDTVAELVAEEVTRELLTPSEGTGHRQVFAPAEESQTFLQLTALCQDRTLVGMKLLEKISSVPHGELSCTTELLILAHHCFTLTCHMEGIMRVLQAARLLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDCVKDGQRLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRPSVFEEISIKCKHHQPTEFVLKNLKKLLTYCEDIYLYYKLAYEHKLYDVVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_864766.6
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_864766.6

ORF Insert Sequence:

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT 
AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC 
GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC 
GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC 
AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG 
GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC 
AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC 
ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC 
AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG 
AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA 
TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC 
TGTCTAAAGG ACATGCTGGC CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_002691092.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691138.2 Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

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CloneID	OBa06715
Clone ID Related Accession (Same CDS sequence)	XM_002691092.5 , XM_864766.6 , XM_002691092.4
Accession Version	XM_002691092.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104285.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT 
AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC 
GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC 
GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC 
AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG 
GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC 
AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC 
ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC 
AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG 
AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA 
TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC 
TGTCTAAAGG ACATGCTGGC CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691138.2
CDS	49..7335
Translation	MAAEQGALPVLLVPVPAEAVGRLGSRAQLHREQAALGSLTAAGSLHVLPLAPGGRGGGDCRLEGCFRQFVWEDSHHSGTPNNKPRLLALTENYELLICEFNLEHGRCDATILYSYSEETLQKLVEDQNISISLLSLRILSFHNNTSLLFINKCVVLHVMFPERDAEIRVLSCCTLPLPAQAVDRIIDTQLCRGILFILSSLGWIYIFDIVDGKQVAHVDLALAQEDMCNEQQQESAKSSSFTSLKVSQDLDVVVVVGFSNTAVALNLTLYFRQYPGHLLCEKTLQDSPIEGPKGIDEDDPVNSDYNMKLTKFSFRIDRSWKAQLSSLNETIKSSKLGGSCCAPWFQDISHLESPESGNRSTSVSDWAFIPQDVTCSPCNFPQKGPARTSDPGRVWKRMHIREQEEPLELTCVSVTGFTALFTWAGESTGWTIVLWDLETQSTQCFSLGKKCIPVDSGGVQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSSVPDQFDHFASHLYLKHVEELTPALDLLCSAIRENDSETQSKHFSEQLLNLTLSFLNKQIKELFIHIEELDEHLQKGVDILTSYIDELRTFMIKFPWKLTDAIDEYDVNENVPKVKESNIWEKLSVEEVIANAILNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNMEEASELLKNMGFNVKDQLLKICFYTADKSVRDFLVEIFKEKNYFSEKEKRTVDFVHQVEKFYSGHLQENIQIQSLPRYWIKEQDFSKHKSVLDSFLKYDHKDKLNKQDHRIVLNWAQWWNQPTQESILLPWINPEGYNSYSPEGLWRYLTARHDWPSIILWIGEFQTQSSYASLEQNKWPPLTVDVVDQNTCCNNYMRNEILDKLARNGIFLTSELEDFELFLVRLNRVGGVMQDALAVQNYRSTEGWGFHSHFILYCLEHSLQYLLYVYLDHYKLSPPNCPFLGKKELHEAHPWFEFLVQCRQVSSNLTDPRLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNGENENSLKKVDPQLLKMALTPYPKLKAALFPQYTAPSVLPPDITLYHLIQSLLPFDPSRLFGWQSANTLAIGDAASQLPHFSSPDLVNKYAVVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGHEAYVLGLSCFHISSIGAACVCFLELLGLDSLKLRVDMKVANIILNYKCGNEDAPYNSIRESLTEKLSKLALGERATIEELLVLLEEGIWSNIQQQQIQRLSSESSSQWALVVQFCRLHNIKPSTSYLRECARANDWLQFIIHSQLHNHHPEEVKYLLQYFSPVLQDHLRLAFENLPSVSNSRMDSDQVCNRSSQELQRHKEEMTDFFQILLQCSEVPDSWCWLLGEAVRQKAPILSVLASCLQDASALSCLCVWIIASVEPHVAAEAVGPLQDSAEGHVWGLEDLSVIWRTLLTRQKIRTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLVEFQQSLETLETAATRVPAAIPAPWLKEHVCFLLKLMLQQCSTQYELGRLLQLFVGIEHLFSDGPDVKKLCVLSQILKDTPIAINRAIITSYSLENFQRECRSVLEKLETDGHFALARRVAELAELPVDDLVIEEITQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSVSSRAASSFFSAQALWAGECPAAGGPSSAEERHLLLTLAGHWLAREDPVPLEDLEGLEKQIWLCRVAQHTLRGSQEEGGPRAPQQASAGGELSFDGLASEFSFSNLAVLNTSKYLELTGFPSRDICDNELNWKEQASLNCLIGRLLDDGCVHEASRACRYFRFYHQDVALVLHCRALASGEASVDDLHPEILALLRSTELPEEEEEAGIPLRKVQSTSSLDSWSLVMLPPADEVVTNLETLTSKCLRGKNYCRQVLCLYELAKELGCSYADVAAQDGEAVLRAILASQQPNRCKRAQAFISTQGLRPDTVAELVAEEVTRELLTPSEGTGHRQVFAPAEESQTFLQLTALCQDRTLVGMKLLEKISSVPHGELSCTTELLILAHHCFTLTCHMEGIMRVLQAARLLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDCVKDGQRLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRPSVFEEISIKCKHHQPTEFVLKNLKKLLTYCEDIYLYYKLAYEHKLYDVVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_002691092.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691092.4

ORF Insert Sequence:

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTCA TGAGGAAGAA GCTGGACCCT 
AGTGGCACCC TGAAGACGGC CCTGCTGGAC TACATCAAGC GCTGCCGCCC TGGGGACAGC 
GAGAAGCACA ACATGATCGC GCTGTGCTTC AGCATGTGCC GCGAGATCGG CGAAAACCAC 
GAGGCAGCTG CCTGCATCCA ACTGAAACTG ATCGAGTCTC AGCCCTGGGA GGACTGTGTC 
AAGGATGGGC AGCGACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGACGCG 
GCAGAGAGTT ATGCCAAGGA CTCCTGCGTG CGCCAGGCCC TGCACTGTCA CCGGCTCACC 
AAGTTGATAA CGCTGCAGAT TCACTTTCTG AACACTGGCC AAAACACGAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGGCGCTGC CTCGGTTCTA CCAGGCTTCC 
ATTGTGGCAG AGGCCTATGA CTTTGTTCCT GACTGGGCTG AAATTCTCTA TCAACAAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAATTTAAGC AGCAAAGGTT ATTAAGACCC 
AGTGTATTTG AAGAGATTTC AATAAAATGT AAACATCATC AGCCTACAGA ATTTGTTCTG 
AAAAACCTGA AGAAGTTACT CACATATTGT GAAGACATCT ACTTGTATTA CAAGCTGGCA 
TACGAACACA AACTTTACGA TGTTGTAAAT GTGCTTCTGA AGGACCCTCA GACCGGCTGC 
TGTCTAAAGG ACATGCTGGC CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_015465259.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320745.1 Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

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CloneID	OBa46952
Clone ID Related Accession (Same CDS sequence)	XM_015465259.1 , XM_010809702.2
Accession Version	XM_015465259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320745.1
CDS	51..6521
Translation	MAAEQGALPVLLVPVPAEAVGRLGSRAQLHREQAALGSLTAAGSLHVLPLAPGGRGGGDCRLEGCFRQFVWEDSHHSGTPNNKPRLLALTENYELLICEFNLEHGRCDATILYSYSEETLQKLVEDQNISISLLSLRILSFHNNTSLLFINKCVVLHVMFPERDAEIRVLSCCTLPLPAQAVDRIIDTQLCRGILFILSSLGWIYIFDIVDGKQVAHVDLALAQEDMCNEQQQESAKSSSFTSLKVSQDLDVVVVVGFSNTAVALNLTLYFRQYPGHLLCEKTLQDSPIEGPKGIDEDDPVNSDYNMKLTKFSFRIDRSWKAQLSSLNETIKSSKLGGSCCAPWFQDISHLESPESGNRSTSVSDWAFIPQDVTCSPCNFPQKGPARTSDPGRVWKRMHIREQEEPLELTCVSVTGFTALFTWAGESTGWTIVLWDLETQSTQCFSLGKKCIPVDSGGVQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSSVPDQFDHFASHLYLKHVEELTPALDLLCSAIRENDSETQSKHFSEQLLNLTLSFLNKQIKELFIHIEELDEHLQKGVDILTSYIDELRTFMIKFPWKLTDAIDEYDVNENVPKVKESNIWEKLSVEEVIANAILNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNMEEASELLKNMGFNVKDQLLKICFYTADKSVRDFLVEIFKEKNYFSEKEKRTVDFVHQVEKFYSGHLQENIQIQSLPRYWIKEQDFSKHKSVLDSFLKYDHKDKLNKQDHRIVLNWAQWWNQPTQESILLPWINPEGYNSYSPEGLWRYLTARHDWPSIILWIGEFQTQSSYASLEQNKWPPLTVDVVDQNTCCNNYMRNEILDKLARNGIFLTSELEDFELFLVRLNRVGGVMQDALAVQNYRSTEGWGFHSHFILYCLEHSLQYLLYVYLDHYKLSPPNCPFLGKKELHEAHPWFEFLVQCRQVSSNLTDPRLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNGENENSLKKVDPQLLKMALTPYPKLKAALFPQYTAPSVLPPDITLYHLIQSLLPFDPSRLFGWQSANTLAIGDAASQLPHFSSPDLVNKYAVVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGHEAYVLGLSCFHISSIGAACVCFLELLGLDSLKLRVDMKVANIILNYKCGNEDAPYNSIRESLTEKLSKLALGERATIEELLVLLEEGIWSNIQQQQIQRLSSESSSQWALVVQFCRLHNIKPSTSYLRECARANDWLQFIIHSQLHNHHPEEVKYLLQYFSPVLQDHLRLAFENLPSVSNSRMDSDQVCNRSSQELQRHKEEMTDFFQILLQCSEVPDSWCWLLGEAVRQKAPILSVLASCLQDASALSCLCVWIIASVEPHVAAEAVGPLQDSAEGHVWGLEDLSVIWRTLLTRQKIRTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLVEFQQSLETLETAATRVPAAIPAPWLKEHVCFLLKLMLQQCSTQYELGRLLQLFVGIEHLFSDGPDVKKLCVLSQILKDTPIAINRAIITSYSLENFQRECRSVLEKLETDGHFALARRVAELAELPVDDLVIEEITQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSVSSRAASSFFSAQALWAGECPAAGGPSSAEERHLLLTLAGHWLAREDPVPLEDLEGLEKQIWLCRVAQHTLRGSQEEGGPRAPQQASAGGELSFDGLASEFSFSNLAVLNTSKYLELTGFPSRDICDNELNWKEQASLNCLIGRLLDDGCVHEASRACRYFRFYHQDVALVLHCRALASGEASVDDLHPEILALLRSTELPEEEEEAGIPLRKVQSTSSLDSWSLVMLPPADEVVTNLETLTSKCLRGKNYCRQVLCLYELAKELGCSYADVAAQDGEAVLRAILASQQPNRCKRAQAFISTQGLRPDTVAELVAEEVTRELLTPSEGTGHRQVFAPAEESQTFLQLTALCQDRTLVGMKLLEKISSVPHGELSCTTELLILAHHCFTLTCHMEGIMRVLQAARLLTDNHLAPSEEYGLVVRLLTGIGRVAP

Target ORF information:

RefSeq Version	XM_015465259.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465259.1

ORF Insert Sequence:

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_010809702.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808004.1 Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

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CloneID	OBa46952
Clone ID Related Accession (Same CDS sequence)	XM_015465259.1 , XM_010809702.2
Accession Version	XM_010809702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808004.1
CDS	51..6521
Translation	MAAEQGALPVLLVPVPAEAVGRLGSRAQLHREQAALGSLTAAGSLHVLPLAPGGRGGGDCRLEGCFRQFVWEDSHHSGTPNNKPRLLALTENYELLICEFNLEHGRCDATILYSYSEETLQKLVEDQNISISLLSLRILSFHNNTSLLFINKCVVLHVMFPERDAEIRVLSCCTLPLPAQAVDRIIDTQLCRGILFILSSLGWIYIFDIVDGKQVAHVDLALAQEDMCNEQQQESAKSSSFTSLKVSQDLDVVVVVGFSNTAVALNLTLYFRQYPGHLLCEKTLQDSPIEGPKGIDEDDPVNSDYNMKLTKFSFRIDRSWKAQLSSLNETIKSSKLGGSCCAPWFQDISHLESPESGNRSTSVSDWAFIPQDVTCSPCNFPQKGPARTSDPGRVWKRMHIREQEEPLELTCVSVTGFTALFTWAGESTGWTIVLWDLETQSTQCFSLGKKCIPVDSGGVQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSSVPDQFDHFASHLYLKHVEELTPALDLLCSAIRENDSETQSKHFSEQLLNLTLSFLNKQIKELFIHIEELDEHLQKGVDILTSYIDELRTFMIKFPWKLTDAIDEYDVNENVPKVKESNIWEKLSVEEVIANAILNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNMEEASELLKNMGFNVKDQLLKICFYTADKSVRDFLVEIFKEKNYFSEKEKRTVDFVHQVEKFYSGHLQENIQIQSLPRYWIKEQDFSKHKSVLDSFLKYDHKDKLNKQDHRIVLNWAQWWNQPTQESILLPWINPEGYNSYSPEGLWRYLTARHDWPSIILWIGEFQTQSSYASLEQNKWPPLTVDVVDQNTCCNNYMRNEILDKLARNGIFLTSELEDFELFLVRLNRVGGVMQDALAVQNYRSTEGWGFHSHFILYCLEHSLQYLLYVYLDHYKLSPPNCPFLGKKELHEAHPWFEFLVQCRQVSSNLTDPRLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNGENENSLKKVDPQLLKMALTPYPKLKAALFPQYTAPSVLPPDITLYHLIQSLLPFDPSRLFGWQSANTLAIGDAASQLPHFSSPDLVNKYAVVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGHEAYVLGLSCFHISSIGAACVCFLELLGLDSLKLRVDMKVANIILNYKCGNEDAPYNSIRESLTEKLSKLALGERATIEELLVLLEEGIWSNIQQQQIQRLSSESSSQWALVVQFCRLHNIKPSTSYLRECARANDWLQFIIHSQLHNHHPEEVKYLLQYFSPVLQDHLRLAFENLPSVSNSRMDSDQVCNRSSQELQRHKEEMTDFFQILLQCSEVPDSWCWLLGEAVRQKAPILSVLASCLQDASALSCLCVWIIASVEPHVAAEAVGPLQDSAEGHVWGLEDLSVIWRTLLTRQKIRTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLVEFQQSLETLETAATRVPAAIPAPWLKEHVCFLLKLMLQQCSTQYELGRLLQLFVGIEHLFSDGPDVKKLCVLSQILKDTPIAINRAIITSYSLENFQRECRSVLEKLETDGHFALARRVAELAELPVDDLVIEEITQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSVSSRAASSFFSAQALWAGECPAAGGPSSAEERHLLLTLAGHWLAREDPVPLEDLEGLEKQIWLCRVAQHTLRGSQEEGGPRAPQQASAGGELSFDGLASEFSFSNLAVLNTSKYLELTGFPSRDICDNELNWKEQASLNCLIGRLLDDGCVHEASRACRYFRFYHQDVALVLHCRALASGEASVDDLHPEILALLRSTELPEEEEEAGIPLRKVQSTSSLDSWSLVMLPPADEVVTNLETLTSKCLRGKNYCRQVLCLYELAKELGCSYADVAAQDGEAVLRAILASQQPNRCKRAQAFISTQGLRPDTVAELVAEEVTRELLTPSEGTGHRQVFAPAEESQTFLQLTALCQDRTLVGMKLLEKISSVPHGELSCTTELLILAHHCFTLTCHMEGIMRVLQAARLLTDNHLAPSEEYGLVVRLLTGIGRVAP

Target ORF information:

RefSeq Version	XM_010809702.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809702.2

ORF Insert Sequence:

ATGGCTGCGG AGCAGGGCGC GCTGCCCGTT TTGTTGGTGC CGGTCCCCGC CGAGGCCGTA 
GGGCGACTGG GGTCTCGGGC GCAGCTGCAC AGAGAGCAGG CGGCCCTGGG GAGTCTGACG 
GCCGCAGGCA GCCTTCACGT ACTTCCCTTG GCGCCGGGCG GCCGAGGCGG GGGCGACTGC 
CGCCTGGAAG GCTGTTTTCG GCAGTTTGTG TGGGAGGATT CTCATCACAG CGGGACACCA 
AACAACAAGC CCAGACTGCT TGCTCTTACT GAAAATTATG AACTGCTCAT CTGTGAATTT 
AATTTGGAAC ATGGAAGATG TGATGCAACC ATTTTATATA GCTATAGTGA AGAGACATTG 
CAGAAGCTTG TTGAAGATCA AAATATCAGT ATTTCCTTAT TATCTTTGAG GATTCTGTCA 
TTTCACAACA ACACATCATT ATTGTTCATC AACAAATGTG TTGTCCTACA TGTTATGTTT 
CCTGAAAGAG ATGCTGAGAT TAGGGTACTC AGCTGTTGCA CATTACCCTT ACCTGCGCAG 
GCAGTGGACA GGATTATTGA CACGCAGCTC TGCAGAGGAA TTCTCTTTAT TTTGAGTAGT 
TTAGGCTGGA TCTACATTTT TGACATTGTG GATGGTAAAC AAGTAGCTCA TGTGGATTTA 
GCACTTGCTC AAGAAGATAT GTGTAATGAG CAGCAACAGG AGTCAGCCAA GAGTTCTTCA 
TTTACTTCAC TGAAAGTGTC TCAGGACCTG GATGTTGTAG TGGTCGTGGG CTTCTCCAAT 
ACTGCAGTTG CTCTTAACTT GACTTTGTAC TTCAGGCAAT ATCCAGGACA TCTGTTATGT 
GAAAAAACTC TACAAGACAG CCCTATTGAA GGTCCCAAGG GGATAGATGA AGATGATCCT 
GTTAATTCTG ACTACAACAT GAAACTGACC AAGTTTTCCT TCCGAATTGA TAGGTCTTGG 
AAAGCCCAGC TGTCATCATT GAATGAAACA ATAAAGAGCT CCAAGCTGGG AGGTTCCTGC 
TGTGCTCCGT GGTTCCAGGA TATTTCGCAT CTGGAGTCCC CTGAATCTGG TAACCGCAGT 
ACCAGTGTGT CAGACTGGGC CTTCATTCCA CAGGATGTGA CATGTAGCCC ATGTAATTTT 
CCACAGAAAG GCCCTGCCAG GACCAGTGAT CCAGGAAGAG TGTGGAAAAG GATGCACATC 
AGGGAACAGG AGGAGCCCCT TGAGCTTACT TGTGTGTCTG TGACAGGCTT CACTGCACTG 
TTTACTTGGG CAGGGGAAAG CACAGGCTGG ACCATTGTCC TGTGGGACTT GGAAACCCAG 
AGCACGCAGT GTTTTTCACT GGGCAAAAAA TGTATTCCTG TAGACAGTGG TGGAGTCCAG 
CAGCTGTGCC TCGTCTTGAC AGAAAGTGGA CTCTCTCTGA TTTTGTTTGG TTTGACTCAA 
GAGGAATTTT TAAATAGGCT GATGATCCAC GGAAGTGCCA GTACTGTGGA CTCCCTTTGT 
CATCTCAATG GTTGGGGGCG GTGTTCCATC CCCATACATG CACTAGAGGC AGGAATAGAA 
AATCGTCAGC TGGACACAGT AGATTTCTTT TTAAAGAGCA AAGAAAATCT TTTGACTCCA 
TCCTCAAAAT CTTCTGTACC TGATCAGTTT GATCACTTTG CATCCCATTT ATATTTAAAA 
CATGTGGAAG AGCTGACACC AGCATTGGAT TTACTTTGCT CAGCAATTAG AGAAAATGAT 
TCTGAGACCC AAAGCAAACA CTTTTCAGAA CAGTTACTTA ACCTTACACT GTCTTTTCTT 
AACAAACAAA TAAAGGAGCT TTTTATTCAC ATTGAAGAGC TAGATGAACA TCTACAGAAA 
GGAGTGGACA TTTTGACCAG CTACATTGAT GAACTTCGAA CCTTCATGAT AAAGTTTCCC 
TGGAAGCTAA CAGATGCTAT AGATGAATAT GATGTAAATG AAAATGTCCC CAAAGTAAAG 
GAGAGCAATA TATGGGAGAA ACTCAGTGTT GAGGAAGTTA TTGCCAATGC CATTTTAAAC 
AACAAAATAC CAGAGGCACA AACTTTCTTC AGGATTAACA GTCATTCTGC TCAAAGACTT 
GAGGAGTTGA TTAGAATAGG CCTAGATTTG GTCTTTGACA GTTTGAAAAA GAACAACATG 
GAGGAAGCCT CTGAGCTTTT GAAGAATATG GGCTTTAATG TAAAAGACCA GTTGCTCAAG 
ATATGCTTCT ATACAGCTGA TAAAAGCGTA CGGGACTTTC TGGTTGAAAT TTTTAAAGAA 
AAAAATTATT TTTCTGAAAA AGAGAAAAGG ACTGTAGACT TTGTGCATCA AGTTGAGAAG 
TTTTATTCAG GACATTTACA AGAAAATATA CAGATCCAGT CTTTACCCAG GTATTGGATA 
AAGGAACAAG ATTTTTCCAA GCACAAGTCT GTTTTGGACT CATTCCTAAA ATATGATCAT 
AAAGATAAAC TTAACAAGCA AGATCATAGG ATTGTGTTAA ATTGGGCTCA GTGGTGGAAT 
CAGCCAACAC AAGAATCCAT CCTTCTCCCC TGGATAAATC CAGAAGGATA TAACTCGTAT 
TCTCCTGAAG GCCTCTGGAG ATACCTCACA GCACGCCATG ATTGGCCCAG CATTATCCTG 
TGGATTGGAG AATTTCAGAC CCAGAGTAGT TATGCTTCAC TTGAGCAGAA TAAATGGCCA 
CCTCTGACTG TTGATGTTGT TGATCAGAAT ACTTGTTGCA ACAACTACAT GAGAAATGAA 
ATTTTAGATA AGCTGGCCAG GAATGGAATT TTTCTTACAT CTGAGTTAGA AGACTTTGAA 
CTCTTCCTTG TCAGACTAAA CCGTGTTGGG GGTGTCATGC AGGATGCCCT CGCTGTTCAG 
AACTATAGGT CCACAGAAGG ATGGGGTTTC CACTCGCACT TCATTCTCTA TTGTTTGGAA 
CACAGTCTAC AGTATCTTCT TTATGTCTAT CTTGACCATT ACAAACTTAG TCCTCCAAAT 
TGTCCTTTTT TGGGAAAAAA AGAATTACAT GAAGCTCACC CTTGGTTTGA ATTTTTAGTT 
CAGTGTCGAC AAGTTTCCAG TAATTTAACA GATCCCAGAC TGATCTTCCA GGCTAGCCTT 
GCAAATGCTC AGATACTGAT TCCCAGCAAC CAGGCCAGTG TCAGCAGTAT GCTATTGGAA 
GGACATACCC TCCTAGCCCT TGCTACCACA ATGTATGCCC CTGGGGGCGT CAGCCAGGTT 
GTTCAGAATG GAGAAAATGA GAACTCTCTG AAGAAAGTAG ATCCCCAGCT ACTGAAGATG 
GCATTAACTC CTTACCCAAA GCTTAAAGCT GCTCTGTTCC CACAGTACAC CGCCCCTAGT 
GTCCTGCCTC CCGACATTAC ACTCTACCAC CTCATTCAGT CATTGTTGCC CTTTGATCCT 
AGCAGATTGT TTGGCTGGCA GTCTGCCAAC ACATTGGCTA TAGGAGATGC AGCGAGTCAG 
CTCCCACATT TCTCTAGCCC TGACCTGGTT AATAAATACG CTGTAGTAGA ACGTCTGAAT 
TTTGCTTATT ATTTACATCA TGGGCGGCCA TCATTTGCAT TTGGTACTTT TCTGGTTCAG 
GAATTAACCA AGAGCAAGAC CCCCAAGCAG CTGATCCAGC AAGTCGGCCA TGAAGCCTAT 
GTTTTAGGGC TCTCCTGCTT CCACATATCT TCGATAGGAG CCGCCTGTGT ATGCTTCTTA 
GAATTGCTTG GTCTCGACAG CCTCAAGCTC AGAGTTGATA TGAAAGTGGC CAATATAATT 
TTGAACTACA AGTGTGGAAA TGAAGATGCT CCGTACAACT CTATCAGAGA GTCTCTAACT 
GAAAAACTGT CTAAACTTGC TCTGGGTGAA AGGGCAACCA TCGAAGAGTT ACTTGTTCTC 
TTAGAAGAAG GCATATGGAG CAACATTCAG CAGCAGCAAA TACAGAGGTT ATCCAGTGAA 
TCCAGCAGCC AGTGGGCATT AGTGGTACAG TTCTGCAGGC TCCACAATAT AAAACCGAGC 
ACATCTTACC TTAGAGAATG TGCCAGAGCA AATGACTGGT TACAGTTTAT TATTCATAGC 
CAGCTCCATA ATCACCATCC AGAGGAGGTG AAATATCTTC TCCAGTATTT CAGCCCTGTT 
CTTCAGGACC ACTTAAGACT GGCTTTTGAG AACTTGCCCT CAGTGTCCAA CTCCAGAATG 
GACAGTGACC AAGTCTGCAA CAGGTCCTCA CAGGAACTCC AGAGACACAA AGAAGAGATG 
ACAGATTTCT TTCAGATTCT GCTTCAGTGT TCAGAGGTGC CAGACTCCTG GTGCTGGCTC 
CTGGGAGAAG CAGTGAGACA AAAGGCCCCA ATCCTCAGTG TCCTGGCCTC ATGTCTCCAG 
GATGCCAGTG CTCTCTCTTG TCTCTGTGTC TGGATCATCG CCTCTGTGGA GCCCCACGTT 
GCAGCTGAAG CAGTGGGACC CCTTCAGGAC TCAGCGGAGG GCCACGTCTG GGGCCTGGAG 
GACCTCTCCG TCATCTGGAG GACATTGCTA ACAAGGCAGA AGATCAGGAC TCTCATCAGA 
GGTTTCCAGC TTTTCTTTAA GGATTCCCCA CTCCTGTTGA TGATGGAGAT GTATGAACTC 
TGTATGTTCT TCAAGAATTA TAAGGAGGCT GAAGCTAAAC TTGTGGAGTT CCAGCAGAGC 
CTTGAAACTC TTGAGACAGC AGCCACAAGG GTCCCCGCTG CCATCCCTGC GCCATGGCTG 
AAGGAACACG TGTGTTTTCT TTTGAAGCTC ATGCTGCAGC AGTGTAGCAC CCAGTATGAG 
CTGGGGAGGC TCTTACAGCT GTTTGTTGGA ATAGAGCATC TCTTCTCTGA TGGTCCAGAT 
GTGAAGAAGC TGTGTGTCCT TAGTCAGATT TTGAAGGATA CACCCATAGC CATTAATCGT 
GCAATCATTA CTAGCTACAG CCTTGAGAAT TTTCAGCGTG AATGTAGGTC AGTTTTGGAA 
AAACTGGAGA CAGACGGACA CTTTGCTTTG GCCAGGAGGG TAGCAGAACT AGCTGAGTTA 
CCTGTGGACG ACTTGGTAAT TGAAGAGATA ACACAGGAAA TGCAGACCTT AAAGCACATT 
GACCAGTGGT CCCTGAAACA AGCACGAATT GACTTCTGGA AAAAATGTCA TGAGAATTTT 
AAGAAAAATT CCGTTTCCAG CAGAGCAGCT TCTTCTTTTT TCTCAGCCCA GGCCCTGTGG 
GCAGGTGAGT GCCCCGCTGC AGGGGGTCCC AGCAGCGCCG AGGAGCGCCA CCTGCTGCTC 
ACGCTGGCAG GGCACTGGCT CGCCCGGGAG GACCCTGTGC CTTTGGAGGA CCTGGAGGGC 
CTGGAGAAGC AGATCTGGCT CTGCCGTGTC GCCCAGCACA CCCTCAGGGG GAGCCAGGAG 
GAAGGAGGGC CCAGAGCGCC TCAGCAGGCC TCAGCTGGAG GTGAACTTTC CTTTGATGGC 
TTAGCCAGTG AGTTTTCATT CTCTAACTTG GCTGTTCTGA ACACATCAAA ATACTTAGAA 
CTTACTGGCT TTCCATCCAG AGATATTTGT GACAATGAAC TGAACTGGAA GGAGCAGGCA 
TCCCTTAACT GTTTGATCGG GCGCCTGCTG GATGACGGCT GTGTGCACGA AGCAAGCCGA 
GCGTGCCGGT ATTTTCGTTT CTATCACCAA GACGTCGCCT TGGTGTTGCA CTGCAGAGCT 
CTGGCTTCAG GGGAGGCTAG TGTGGACGAC CTGCACCCGG AGATCCTCGC GCTCTTACGA 
AGTACTGAGC TTCCTGAGGA AGAAGAGGAA GCTGGTATCC CCCTAAGGAA AGTCCAGAGC 
ACTTCCAGTC TGGACAGTTG GTCGTTAGTG ATGCTGCCCC CCGCGGACGA AGTGGTGACG 
AACCTGGAAA CTCTGACAAG CAAGTGCCTC CGCGGGAAGA ACTACTGCCG GCAGGTCCTC 
TGTCTCTATG AGCTCGCCAA GGAGCTGGGC TGTTCCTACG CAGACGTCGC TGCCCAGGAT 
GGTGAGGCCG TGCTCCGGGC CATCTTGGCT TCTCAGCAGC CTAATCGATG CAAGCGAGCT 
CAAGCCTTCA TCAGCACCCA GGGCCTCAGG CCAGACACAG TGGCTGAACT TGTGGCTGAA 
GAGGTGACCC GGGAGCTGCT GACACCGTCG GAGGGAACAG GACACAGGCA GGTGTTCGCC 
CCAGCAGAGG AGAGCCAGAC GTTTCTTCAG CTGACTGCTT TGTGTCAAGA CCGCACCTTG 
GTGGGCATGA AGCTGTTGGA GAAGATTTCC TCTGTTCCGC ACGGGGAGCT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC TCATCACTGC TTCACACTGA CATGCCACAT GGAGGGCATC 
ATGCGTGTCC TGCAGGCCGC CCGGCTGCTC ACCGACAACC ACTTGGCCCC CAGTGAGGAG 
TACGGGCTGG TGGTGCGCCT CCTCACTGGC ATCGGAAGAG TGGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SPG11 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報