PIK3R4 cDNA ORF clone, Bos taurus(cattle)

The following PIK3R4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIK3R4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa46829 XM_025001065.1
Latest version!
Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa16190 XM_005202130.3
Latest version!
Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa39347 NM_001100314.2
Latest version!
Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa39347 XM_025000996.1
Latest version!
Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa16190 XM_005202130.2 Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa46829
    Clone ID Related Accession (Same CDS sequence) XM_025001065.1
    Accession Version XM_025001065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoinositide 3-kinase regulatory subunit 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGCCC CCTTTCTGTG TCATCCCAAC CTATGGATAC GCTATGGTGC TGTGGGATTT
    ATCACTGTGG TAGCTCACCA AATAAGCACA GCTGATGTCT ACTGCAAACT GATGCCTTAC
    CTTGACCCAT ATATTACTCA GCCAATAATA CAGATTGAAA GAAAACTTGT TCTACTCAGT
    GTTTTAAAAG AGCCAGTAAG TCGTTCTATA TTTGATTATG CTTTGAGGTC TAAAGATATC
    ACTAGCTTGT TCAGACATCT TCACATGCGT CAGAAGAAGC GAAACGGGTC TCTTCCTGAC
    TGCCCTCCAC CCGAGGATCC TGCCATAGCA CAGCTGCTGA AGAAGTTGCT TTCACAGGGA
    ATGACAGAGG AAGAGGAAGA CAAGCTTTTG GCTCTGAAAG ACTTCATGAT GAAATCTAAC
    AAGGCAAAGG CCAATATAGT GGATCAGAGT CATCTCCATG ATAGTAGTCA GAAAGGTGTA
    ATTGATTTGG CAGCCTTAGG TATAACTGGG AGACAAGTTG ATCTTGTTAA AACCAAACAG
    GAACCAGATG ACAAGCGAGC TAGAAAACAT GTTAAACAAG ACTCAAATGT CAATGAAGAG
    TGGAAAAGCA TGTTTGGCTC TCTGGAGCCA CCAAGCATGC AGCAGGCCCT GCCTAAAGGG
    AGTGATCAGG AGGTGGTGCC GGCTGGGAAG CCGCCTCGTT CCGAGTCCTC CGCTGCTGTC
    TGCGTCCCTT TGTCGACTTC TCCACAGATT CCAGAAGTGA CAAATGTCCA AAATAGAAAA
    CCAACAATAC AGGTTTTAAG TAGTACAATT TTACCGTCCA CTTACCAGAT CCGGATCACA
    ACGTGTAAAA CTGAGCTTCA GCAGCTCATA CAACAGAAAC GGGAGCAGTG CAATGCCGAG
    AGGATAGCTA AGCAGATGAT GGAAAACGCT GAGTGGGAGA GTAAACCCCC CCCACCGGGG
    TGGCGGCCTA AGGGGCTACT TGTGGCACAT CTTCACGAAC ATAAGTCTGC TGTGAATCGG
    ATTAGAGTCT CTGACGAGCA TTCACTGTTT GCCACGTGTT CAAATGATGG CACAGTGAAA
    ATCTGGAACA GTCAGAAGAT GGAGGGGAAG ACCACCACCA CCAGGTCTAT TCTCACCTAT
    AGCCGAGTCG GGGGCTGTGT CAAGACGCTC ACATTCTGCC AGGGTTCCCA CTACTTAGCC
    GTAGCGTCCG ATAATGGTGC CGTCCAGCTT CTCGCGATTG AGGCCTCCAA GCTGCCCAAG
    TCTCCCAAGA TTCATCCTCT GCAAAGCAGA GTCCTAGACC AGAAGGAGGA CGGCTGCGTG
    GTGGACCTGC ATCACTTCAG CTCCGGGGCG CAGTCCGTGC TGGCCTACGC CACTGTGAAT
    GGTGCTCTGG TGGGCTGGGA CCTGCGCTCC TCCAGCAACG CCTGGACGCT GAGGCACGAT
    CTCAAGGCAG GCCTCATCAC CTCCTTTGCC GTGGACATCC ACCAGTGCTG GCTCTGCATC
    GGCACGAGCA GCGGCACCAT GGCCTGCTGG GACATGCGCT TCCAGCTGCC CATCTCCAGT
    CACTGCCACC CGTCACGGGC CCGCGTCCGG CGCCTCTCCA TGCACCCGCT CTACCAGTCC
    TGGGTGATCG CAGCTGTCCA GGGCAACAAC GAGGTGTCCA TGTGGGACAT GGAGACAGGC
    GACCGAAGGT TCACGCTCTG GGCCAGCAGT GCTCCCCCGC TGTCTGAGTT ACAGCCTTCT
    TCTCACAGTG TCCACGGGCT CTACTGTAGT CCTGCGGATG GGAACCCCAT CCTGCTAACG
    GCCGGCTCAG ACATGAAAAT AAGGTTTTGG GACCTGGCCT ACCCAGAGAG GTCCTACATC
    GTCGCGGGGA GCGCGGGCTC CCCGTCCGTG TCCTACTACA GGAAGGTCAT TGAAGGCACT
    GAAGTTGTCC AGGAGATTCA GAACAAGCAG AAGGTGGGAC CGAGTGACGA CGGCCCACGG
    AGGGGCCCAG AGTCCCTGCC TGTGGGCCAC CACGATATCA TCACGGACGT CGCCACCTTC
    CAGACCACGC AGGGCTTCAT CGTGACCGCC TCTAGGGACG GCATTGTTAA GGTTTGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856833.1
    CDS496..4398
    Translation

    Target ORF information:

    RefSeq Version XM_025001065.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGCCC CCTTTCTGTG TCATCCCAAC CTATGGATAC GCTATGGTGC TGTGGGATTT
    ATCACTGTGG TAGCTCACCA AATAAGCACA GCTGATGTCT ACTGCAAACT GATGCCTTAC
    CTTGACCCAT ATATTACTCA GCCAATAATA CAGATTGAAA GAAAACTTGT TCTACTCAGT
    GTTTTAAAAG AGCCAGTAAG TCGTTCTATA TTTGATTATG CTTTGAGGTC TAAAGATATC
    ACTAGCTTGT TCAGACATCT TCACATGCGT CAGAAGAAGC GAAACGGGTC TCTTCCTGAC
    TGCCCTCCAC CCGAGGATCC TGCCATAGCA CAGCTGCTGA AGAAGTTGCT TTCACAGGGA
    ATGACAGAGG AAGAGGAAGA CAAGCTTTTG GCTCTGAAAG ACTTCATGAT GAAATCTAAC
    AAGGCAAAGG CCAATATAGT GGATCAGAGT CATCTCCATG ATAGTAGTCA GAAAGGTGTA
    ATTGATTTGG CAGCCTTAGG TATAACTGGG AGACAAGTTG ATCTTGTTAA AACCAAACAG
    GAACCAGATG ACAAGCGAGC TAGAAAACAT GTTAAACAAG ACTCAAATGT CAATGAAGAG
    TGGAAAAGCA TGTTTGGCTC TCTGGAGCCA CCAAGCATGC AGCAGGCCCT GCCTAAAGGG
    AGTGATCAGG AGGTGGTGCC GGCTGGGAAG CCGCCTCGTT CCGAGTCCTC CGCTGCTGTC
    TGCGTCCCTT TGTCGACTTC TCCACAGATT CCAGAAGTGA CAAATGTCCA AAATAGAAAA
    CCAACAATAC AGGTTTTAAG TAGTACAATT TTACCGTCCA CTTACCAGAT CCGGATCACA
    ACGTGTAAAA CTGAGCTTCA GCAGCTCATA CAACAGAAAC GGGAGCAGTG CAATGCCGAG
    AGGATAGCTA AGCAGATGAT GGAAAACGCT GAGTGGGAGA GTAAACCCCC CCCACCGGGG
    TGGCGGCCTA AGGGGCTACT TGTGGCACAT CTTCACGAAC ATAAGTCTGC TGTGAATCGG
    ATTAGAGTCT CTGACGAGCA TTCACTGTTT GCCACGTGTT CAAATGATGG CACAGTGAAA
    ATCTGGAACA GTCAGAAGAT GGAGGGGAAG ACCACCACCA CCAGGTCTAT TCTCACCTAT
    AGCCGAGTCG GGGGCTGTGT CAAGACGCTC ACATTCTGCC AGGGTTCCCA CTACTTAGCC
    GTAGCGTCCG ATAATGGTGC CGTCCAGCTT CTCGCGATTG AGGCCTCCAA GCTGCCCAAG
    TCTCCCAAGA TTCATCCTCT GCAAAGCAGA GTCCTAGACC AGAAGGAGGA CGGCTGCGTG
    GTGGACCTGC ATCACTTCAG CTCCGGGGCG CAGTCCGTGC TGGCCTACGC CACTGTGAAT
    GGTGCTCTGG TGGGCTGGGA CCTGCGCTCC TCCAGCAACG CCTGGACGCT GAGGCACGAT
    CTCAAGGCAG GCCTCATCAC CTCCTTTGCC GTGGACATCC ACCAGTGCTG GCTCTGCATC
    GGCACGAGCA GCGGCACCAT GGCCTGCTGG GACATGCGCT TCCAGCTGCC CATCTCCAGT
    CACTGCCACC CGTCACGGGC CCGCGTCCGG CGCCTCTCCA TGCACCCGCT CTACCAGTCC
    TGGGTGATCG CAGCTGTCCA GGGCAACAAC GAGGTGTCCA TGTGGGACAT GGAGACAGGC
    GACCGAAGGT TCACGCTCTG GGCCAGCAGT GCTCCCCCGC TGTCTGAGTT ACAGCCTTCT
    TCTCACAGTG TCCACGGGCT CTACTGTAGT CCTGCGGATG GGAACCCCAT CCTGCTAACG
    GCCGGCTCAG ACATGAAAAT AAGGTTTTGG GACCTGGCCT ACCCAGAGAG GTCCTACATC
    GTCGCGGGGA GCGCGGGCTC CCCGTCCGTG TCCTACTACA GGAAGGTCAT TGAAGGCACT
    GAAGTTGTCC AGGAGATTCA GAACAAGCAG AAGGTGGGAC CGAGTGACGA CGGCCCACGG
    AGGGGCCCAG AGTCCCTGCC TGTGGGCCAC CACGATATCA TCACGGACGT CGCCACCTTC
    CAGACCACGC AGGGCTTCAT CGTGACCGCC TCTAGGGACG GCATTGTTAA GGTTTGGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16190
    Clone ID Related Accession (Same CDS sequence) XM_005202130.2 , XM_005202130.3
    Accession Version XM_005202130.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phosphoinositide 3-kinase regulatory subunit 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103827.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGCTGTC
    CAGGGCAACA ACGAGGTGTC CATGTGGGAC ATGGAGACAG GCGACCGAAG GTTCACGCTC
    TGGGCCAGCA GTGCTCCCCC GCTGTCTGAG TTACAGCCTT CTTCTCACAG TGTCCACGGG
    CTCTACTGTA GTCCTGCGGA TGGGAACCCC ATCCTGCTAA CGGCCGGCTC AGACATGAAA
    ATAAGGTTTT GGGACCTGGC CTACCCAGAG AGGTCCTACA TCGTCGCGGG GAGCGCGGGC
    TCCCCGTCCG TGTCCTACTA CAGGAAGGTC ATTGAAGGCA CTGAAGTTGT CCAGGAGATT
    CAGAACAAGC AGAAGGTGGG ACCGAGTGAC GACGGCCCAC GGAGGGGCCC AGAGTCCCTG
    CCTGTGGGCC ACCACGATAT CATCACGGAC GTCGCCACCT TCCAGACCAC GCAGGGCTTC
    ATCGTGACCG CCTCTAGGGA CGGCATTGTT AAGGTTTGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202187.1
    CDS501..4445
    Translation

    Target ORF information:

    RefSeq Version XM_005202130.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGCTGTC
    CAGGGCAACA ACGAGGTGTC CATGTGGGAC ATGGAGACAG GCGACCGAAG GTTCACGCTC
    TGGGCCAGCA GTGCTCCCCC GCTGTCTGAG TTACAGCCTT CTTCTCACAG TGTCCACGGG
    CTCTACTGTA GTCCTGCGGA TGGGAACCCC ATCCTGCTAA CGGCCGGCTC AGACATGAAA
    ATAAGGTTTT GGGACCTGGC CTACCCAGAG AGGTCCTACA TCGTCGCGGG GAGCGCGGGC
    TCCCCGTCCG TGTCCTACTA CAGGAAGGTC ATTGAAGGCA CTGAAGTTGT CCAGGAGATT
    CAGAACAAGC AGAAGGTGGG ACCGAGTGAC GACGGCCCAC GGAGGGGCCC AGAGTCCCTG
    CCTGTGGGCC ACCACGATAT CATCACGGAC GTCGCCACCT TCCAGACCAC GCAGGGCTTC
    ATCGTGACCG CCTCTAGGGA CGGCATTGTT AAGGTTTGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16190
    Clone ID Related Accession (Same CDS sequence) XM_005202130.2 , XM_005202130.3
    Accession Version XM_005202130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoinositide 3-kinase regulatory subunit 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGCTGTC
    CAGGGCAACA ACGAGGTGTC CATGTGGGAC ATGGAGACAG GCGACCGAAG GTTCACGCTC
    TGGGCCAGCA GTGCTCCCCC GCTGTCTGAG TTACAGCCTT CTTCTCACAG TGTCCACGGG
    CTCTACTGTA GTCCTGCGGA TGGGAACCCC ATCCTGCTAA CGGCCGGCTC AGACATGAAA
    ATAAGGTTTT GGGACCTGGC CTACCCAGAG AGGTCCTACA TCGTCGCGGG GAGCGCGGGC
    TCCCCGTCCG TGTCCTACTA CAGGAAGGTC ATTGAAGGCA CTGAAGTTGT CCAGGAGATT
    CAGAACAAGC AGAAGGTGGG ACCGAGTGAC GACGGCCCAC GGAGGGGCCC AGAGTCCCTG
    CCTGTGGGCC ACCACGATAT CATCACGGAC GTCGCCACCT TCCAGACCAC GCAGGGCTTC
    ATCGTGACCG CCTCTAGGGA CGGCATTGTT AAGGTTTGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202187.1
    CDS487..4431
    Translation

    Target ORF information:

    RefSeq Version XM_005202130.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGCTGTC
    CAGGGCAACA ACGAGGTGTC CATGTGGGAC ATGGAGACAG GCGACCGAAG GTTCACGCTC
    TGGGCCAGCA GTGCTCCCCC GCTGTCTGAG TTACAGCCTT CTTCTCACAG TGTCCACGGG
    CTCTACTGTA GTCCTGCGGA TGGGAACCCC ATCCTGCTAA CGGCCGGCTC AGACATGAAA
    ATAAGGTTTT GGGACCTGGC CTACCCAGAG AGGTCCTACA TCGTCGCGGG GAGCGCGGGC
    TCCCCGTCCG TGTCCTACTA CAGGAAGGTC ATTGAAGGCA CTGAAGTTGT CCAGGAGATT
    CAGAACAAGC AGAAGGTGGG ACCGAGTGAC GACGGCCCAC GGAGGGGCCC AGAGTCCCTG
    CCTGTGGGCC ACCACGATAT CATCACGGAC GTCGCCACCT TCCAGACCAC GCAGGGCTTC
    ATCGTGACCG CCTCTAGGGA CGGCATTGTT AAGGTTTGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39347
    Clone ID Related Accession (Same CDS sequence) NM_001100314.2 , XM_025000996.1
    Accession Version NM_001100314.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product phosphoinositide 3-kinase regulatory subunit 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC148105.1. On Aug 31, 2012 this sequence version replaced NM_001100314.1. ##Evidence-Data-START## Transcript exon combination :: BC148105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGGCACG
    AGCAGCGGCA CCATGGCCTG CTGGGACATG CGCTTCCAGC TGCCCATCTC CAGTCACTGC
    CACCCGTCAC GGGCCCGCGT CCGGCGCCTC TCCATGCACC CGCTCTACCA GTCCTGGGTG
    ATCGCAGCTG TCCAGGGCAA CAACGAGGTG TCCATGTGGG ACATGGAGAC AGGCGACCGA
    AGGTTCACGC TCTGGGCCAG CAGTGCTCCC CCGCTGTCTG AGTTACAGCC TTCTTCTCAC
    AGTGTCCACG GGCTCTACTG TAGTCCTGCG GATGGGAACC CCATCCTGCT AACGGCCGGC
    TCAGACATGA AAATAAGGTT TTGGGACCTG GCCTACCCAG AGAGGTCCTA CATCGTCGCG
    GGGAGCGCGG GCTCCCCGTC CGTGTCCTAC TACAGGAAGG TCATTGAAGG CACTGAAGTT
    GTCCAGGAGA TTCAGAACAA GCAGAAGGTG GGACCGAGTG ACGACGGCCC ACGGAGGGGC
    CCAGAGTCCC TGCCTGTGGG CCACCACGAT ATCATCACGG ACGTCGCCAC CTTCCAGACC
    ACGCAGGGCT TCATCGTGAC CGCCTCTAGG GACGGCATTG TTAAGGTTTG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093784.1
    CDS501..4577
    Translation

    Target ORF information:

    RefSeq Version NM_001100314.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100314.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGGCACG
    AGCAGCGGCA CCATGGCCTG CTGGGACATG CGCTTCCAGC TGCCCATCTC CAGTCACTGC
    CACCCGTCAC GGGCCCGCGT CCGGCGCCTC TCCATGCACC CGCTCTACCA GTCCTGGGTG
    ATCGCAGCTG TCCAGGGCAA CAACGAGGTG TCCATGTGGG ACATGGAGAC AGGCGACCGA
    AGGTTCACGC TCTGGGCCAG CAGTGCTCCC CCGCTGTCTG AGTTACAGCC TTCTTCTCAC
    AGTGTCCACG GGCTCTACTG TAGTCCTGCG GATGGGAACC CCATCCTGCT AACGGCCGGC
    TCAGACATGA AAATAAGGTT TTGGGACCTG GCCTACCCAG AGAGGTCCTA CATCGTCGCG
    GGGAGCGCGG GCTCCCCGTC CGTGTCCTAC TACAGGAAGG TCATTGAAGG CACTGAAGTT
    GTCCAGGAGA TTCAGAACAA GCAGAAGGTG GGACCGAGTG ACGACGGCCC ACGGAGGGGC
    CCAGAGTCCC TGCCTGTGGG CCACCACGAT ATCATCACGG ACGTCGCCAC CTTCCAGACC
    ACGCAGGGCT TCATCGTGAC CGCCTCTAGG GACGGCATTG TTAAGGTTTG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39347
    Clone ID Related Accession (Same CDS sequence) NM_001100314.2 , XM_025000996.1
    Accession Version XM_025000996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phosphoinositide 3-kinase regulatory subunit 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGGCACG
    AGCAGCGGCA CCATGGCCTG CTGGGACATG CGCTTCCAGC TGCCCATCTC CAGTCACTGC
    CACCCGTCAC GGGCCCGCGT CCGGCGCCTC TCCATGCACC CGCTCTACCA GTCCTGGGTG
    ATCGCAGCTG TCCAGGGCAA CAACGAGGTG TCCATGTGGG ACATGGAGAC AGGCGACCGA
    AGGTTCACGC TCTGGGCCAG CAGTGCTCCC CCGCTGTCTG AGTTACAGCC TTCTTCTCAC
    AGTGTCCACG GGCTCTACTG TAGTCCTGCG GATGGGAACC CCATCCTGCT AACGGCCGGC
    TCAGACATGA AAATAAGGTT TTGGGACCTG GCCTACCCAG AGAGGTCCTA CATCGTCGCG
    GGGAGCGCGG GCTCCCCGTC CGTGTCCTAC TACAGGAAGG TCATTGAAGG CACTGAAGTT
    GTCCAGGAGA TTCAGAACAA GCAGAAGGTG GGACCGAGTG ACGACGGCCC ACGGAGGGGC
    CCAGAGTCCC TGCCTGTGGG CCACCACGAT ATCATCACGG ACGTCGCCAC CTTCCAGACC
    ACGCAGGGCT TCATCGTGAC CGCCTCTAGG GACGGCATTG TTAAGGTTTG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856764.1
    CDS478..4554
    Translation

    Target ORF information:

    RefSeq Version XM_025000996.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGAAACC AGCTCGCTGG CATTGCTCCC TCCCAGATCC TTTCTGTGGA GAGTTATTTC 
    TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGGA GTACTCGGTT CTTTAAAGTT
    GCTCGAGCGA AGCACCGAGA AGGCCTGGTA GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
    ACATTGCCTC TCACCAGCTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTTCATTCT
    GCCCAGAACT GTCTGCCTTT CCAGAAAGCT GCCGAAAAAG CCTCTGAGAA GGCAGCCATG
    CTCTTCAGGC AGTACGTGCG AGACAACCTC TACGACCGAA TCAGCACCCG TCCGTTCCTA
    AATAACATCG AGAAGCGCTG GGTCGCTTTC CAGATCCTCA CAGCTGTGGA CCAAGCGCAC
    AAATCTGGCG TCCGTCATGG GGACATCAAG ACGGAGAATG TGATGGTCAC CAGTTGGAAC
    TGGGTTCTTC TAACGGATTT TGCCAGTTTC AAGCCCACTT ACCTTCCAGA GGACAACCCG
    GCAGATTTCA ACTATTTTTT TGACACTTCA CGGAGGAGAA CTTGTTACAT TGCTCCCGAA
    CGTTTTGTCG ATGGTGGATT GTTTGCCACA GAGTTAGAAT ATATGAGAGA TCCTTCAACT
    CCACTTGTAG ACTTAAACAG CAATCAGAGA ACAAGAGGAG AATTGAAGCG AGCAATGGAT
    ATCTTTTCAG CAGGTTGTGT GATCGCTGAG CTTTTTACAG AAGGCGTGCC TTTGTTTGAT
    CTCTCTCAAC TTTTGGCCTA TAGAAATGGG CAGTTTTTCC CTGAGCAGGT GCTGAGTAAA
    ATTGAAGATC GCAGTATCAG AGAACTGGTA ACTCAGATGA TTCACCGGGA ACCAGATAAA
    CGTTTAGAGG CAGAAGATTA CTTAAAGCAG CAACGTGGCA ATGCCTTTCC TGAAATATTC
    TACACTTTTC TTCAGCCCTA TATGGCCCAG TTTGCCAAAG AAACATTTCT TTCTGCAGAT
    GAGCGTATTC TGGTTATAAG GAAGGATTTG GACAACATCA TTCACAATCT CTGTGGACAC
    GATCTGCTGG AGAAAGCGGA TGGAGAGCCC AAGGAGAATG GGCTGGTTAT CTTGGTGTCA
    GTTATAACGT CCTGCCTGCA GACCCTGAAA TACTGCGACT CCAAGCTTGC CGCTTTGGAA
    CTTATTCTCC ATTTGGCCCC AAGATTAAGT GTAGAAATTC TTTTGGATCG TATTACTCCT
    TATCTTTTAC ATTTCAGCAA TGACTCTGTT CCCAGAGTAA GGGCCGAAGC CTTGCGGACG
    TTGACTAAAG TTCTTGCTCT GGTGAAGGAG GTTCCTCGCA ACGATGTCAA CATTTACCCA
    GAGTACATCC TGCCAGGCAT AGCCCACTTG GCCCAGGATG ACGCCACGAT CGTCAGGCTA
    GCCTACGCTG AAAACATAGC TCTGCTGGCA GAAACAGCTT TGAGATTCCT GGAATTAGTA
    CAGTTGAAAA ATCTCAATAT GGAAAATGAC CCAAATAGTG AAGAAATAGA TGAAGTTACA
    CATCCCAATG GGAATTATGA CACAGAGCTC CAAGCCTTAC ATGAAATGGT CCAGCAGAAA
    GTTGTCACTT TGCTAAGTGA CCCTGAAAAT ATCGTGAAAC AAACCTTAAT GGAAAATGGG
    ATAACACGGC TGTGCGTATT CTTTGGACGT CAGAAAGCTA ATGATGTTTT GCTGTCCCAC
    ATGATCACTT TCCTGAATGA TAAAAATGAT TGGCATCTTC GTGGAGCTTT TTTTGATAGT
    ATAGTTGGCG TCGCTGCCTA TGTTGGCTGG CAGAGCTCCT CAATTCTCAA ACCCCTGCTG
    CAACAAGGTC TTAGTGATGC TGAGGAATTT GTTATTGTGA AAGCTCTTAA CGCCCTTACC
    TGTATGTGTC AATTAGGGCT GCTGCAAAAA CCCCATGTCT ACGAGTTTGC CAGTGATATT
    GCCCCCTTTC TGTGTCATCC CAACCTATGG ATACGCTATG GTGCTGTGGG ATTTATCACT
    GTGGTAGCTC ACCAAATAAG CACAGCTGAT GTCTACTGCA AACTGATGCC TTACCTTGAC
    CCATATATTA CTCAGCCAAT AATACAGATT GAAAGAAAAC TTGTTCTACT CAGTGTTTTA
    AAAGAGCCAG TAAGTCGTTC TATATTTGAT TATGCTTTGA GGTCTAAAGA TATCACTAGC
    TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGAAACG GGTCTCTTCC TGACTGCCCT
    CCACCCGAGG ATCCTGCCAT AGCACAGCTG CTGAAGAAGT TGCTTTCACA GGGAATGACA
    GAGGAAGAGG AAGACAAGCT TTTGGCTCTG AAAGACTTCA TGATGAAATC TAACAAGGCA
    AAGGCCAATA TAGTGGATCA GAGTCATCTC CATGATAGTA GTCAGAAAGG TGTAATTGAT
    TTGGCAGCCT TAGGTATAAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAGGAACCA
    GATGACAAGC GAGCTAGAAA ACATGTTAAA CAAGACTCAA ATGTCAATGA AGAGTGGAAA
    AGCATGTTTG GCTCTCTGGA GCCACCAAGC ATGCAGCAGG CCCTGCCTAA AGGGAGTGAT
    CAGGAGGTGG TGCCGGCTGG GAAGCCGCCT CGTTCCGAGT CCTCCGCTGC TGTCTGCGTC
    CCTTTGTCGA CTTCTCCACA GATTCCAGAA GTGACAAATG TCCAAAATAG AAAACCAACA
    ATACAGGTTT TAAGTAGTAC AATTTTACCG TCCACTTACC AGATCCGGAT CACAACGTGT
    AAAACTGAGC TTCAGCAGCT CATACAACAG AAACGGGAGC AGTGCAATGC CGAGAGGATA
    GCTAAGCAGA TGATGGAAAA CGCTGAGTGG GAGAGTAAAC CCCCCCCACC GGGGTGGCGG
    CCTAAGGGGC TACTTGTGGC ACATCTTCAC GAACATAAGT CTGCTGTGAA TCGGATTAGA
    GTCTCTGACG AGCATTCACT GTTTGCCACG TGTTCAAATG ATGGCACAGT GAAAATCTGG
    AACAGTCAGA AGATGGAGGG GAAGACCACC ACCACCAGGT CTATTCTCAC CTATAGCCGA
    GTCGGGGGCT GTGTCAAGAC GCTCACATTC TGCCAGGGTT CCCACTACTT AGCCGTAGCG
    TCCGATAATG GTGCCGTCCA GCTTCTCGCG ATTGAGGCCT CCAAGCTGCC CAAGTCTCCC
    AAGATTCATC CTCTGCAAAG CAGAGTCCTA GACCAGAAGG AGGACGGCTG CGTGGTGGAC
    CTGCATCACT TCAGCTCCGG GGCGCAGTCC GTGCTGGCCT ACGCCACTGT GAATGGTGCT
    CTGGTGGGCT GGGACCTGCG CTCCTCCAGC AACGCCTGGA CGCTGAGGCA CGATCTCAAG
    GCAGGCCTCA TCACCTCCTT TGCCGTGGAC ATCCACCAGT GCTGGCTCTG CATCGGCACG
    AGCAGCGGCA CCATGGCCTG CTGGGACATG CGCTTCCAGC TGCCCATCTC CAGTCACTGC
    CACCCGTCAC GGGCCCGCGT CCGGCGCCTC TCCATGCACC CGCTCTACCA GTCCTGGGTG
    ATCGCAGCTG TCCAGGGCAA CAACGAGGTG TCCATGTGGG ACATGGAGAC AGGCGACCGA
    AGGTTCACGC TCTGGGCCAG CAGTGCTCCC CCGCTGTCTG AGTTACAGCC TTCTTCTCAC
    AGTGTCCACG GGCTCTACTG TAGTCCTGCG GATGGGAACC CCATCCTGCT AACGGCCGGC
    TCAGACATGA AAATAAGGTT TTGGGACCTG GCCTACCCAG AGAGGTCCTA CATCGTCGCG
    GGGAGCGCGG GCTCCCCGTC CGTGTCCTAC TACAGGAAGG TCATTGAAGG CACTGAAGTT
    GTCCAGGAGA TTCAGAACAA GCAGAAGGTG GGACCGAGTG ACGACGGCCC ACGGAGGGGC
    CCAGAGTCCC TGCCTGTGGG CCACCACGAT ATCATCACGG ACGTCGCCAC CTTCCAGACC
    ACGCAGGGCT TCATCGTGAC CGCCTCTAGG GACGGCATTG TTAAGGTTTG GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.