SFTPB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SFTPB
Entrez Gene ID	507398
Full Name	surfactant protein B
General protein information	Preferred Names surfactant protein B Names pulmonary surfactant-associated protein B 6 kDa protein SP-B pulmonary surfactant-associated proteolipid SPL(Phe) surfactant, pulmonary-associated protein B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473479.1	XP_015328965.1	pulmonary surfactant-associated protein B isoform X2
XM_024998422.1	XP_024854190.1	pulmonary surfactant-associated protein B isoform X1
XM_005212760.2	XP_005212817.1	pulmonary surfactant-associated protein B isoform X1
XM_015473479.2	XP_015328965.1	pulmonary surfactant-associated protein B isoform X1
XM_005212760.3	XP_005212817.1	pulmonary surfactant-associated protein B isoform X2
NM_001075311.2	NP_001068779.1	pulmonary surfactant-associated protein B precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SFTPB	XP_005630540.1
	SFTPB	XP_001089841.1
Pan troglodytes (chimpanzee)	SFTPB	XP_001168379.2
Mus musculus (house mouse)	Sftpb	NP_001269000.1
Homo sapiens (human)	SFTPB	NP_000533.3
Xenopus tropicalis (tropical clawed frog)	sftpb	XP_002932589.2
Bos taurus (cattle)	SFTPB	NP_001068779.1
Rattus norvegicus (Norway rat)	Sftpb	NP_620197.1

Gene Ontology
Bibliography

Related articles in PubMed

Orientation and depth of surfactant protein B C-terminal helix in lung surfactant bilayers.
Bertani P, Vidovic V, Yang TC, Rendell J, Gordon LM, Waring AJ, Bechinger B, Booth V
Biochimica et biophysica acta1818(5)1165-72(2012 May)

Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.
Hite RD, Grier BL, Waite BM, Veldhuizen RA, Possmayer F, Yao LJ, Seeds MC
American journal of physiology. Lung cellular and molecular physiology302(2)L257-65(2012 Jan)

Hydrophobic surfactant proteins induce a phosphatidylethanolamine to form cubic phases.
Chavarha M, Khoojinian H, Schulwitz LE Jr, Biswas SC, Rananavare SB, Hall SB
Biophysical journal98(8)1549-57(2010 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Modification of tryptophan and methionine residues is implicated in the oxidative inactivation of surfactant protein B.
Manzanares D, Rodriguez-Capote K, Liu S, Haines T, Ramos Y, Zhao L, Doherty-Kirby A, Lajoie G, Possmayer F
Biochemistry46(18)5604-15(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Measurements of the depth of SP-B(CTERM) indicated the peptide center positions ~8A more deeply than the phosphate headgroups, a topology that may allow the peptide to promote functional lipid structures without causing micellization upon compression.
Title: Orientation and depth of surfactant protein B C-terminal helix in lung surfactant bilayers.

results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface)
Title: Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.

The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases.
Title: Hydrophobic surfactant proteins induce a phosphatidylethanolamine to form cubic phases.

Reactive oxygen species inactivation of lipid extract surfactant B (SP-B) biophysical activity appears to involve modification of a maximum of three amino acids: Trp9, Met29, and Met65.
Title: Modification of tryptophan and methionine residues is implicated in the oxidative inactivation of surfactant protein B.

The following SFTPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFTPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa24032	NM_001075311.2 Latest version!	Bos taurus surfactant protein B (SFTPB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa24032	XM_005212760.3 Latest version!	Bos taurus surfactant protein B (SFTPB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa91888	XM_015473479.2 Latest version!	Bos taurus surfactant protein B (SFTPB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa91888	XM_024998422.1 Latest version!	Bos taurus surfactant protein B (SFTPB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OBa91888	XM_015473479.1	Bos taurus surfactant protein B (SFTPB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa24032	XM_005212760.2	Bos taurus surfactant protein B (SFTPB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SFTPB ( NM_001075311.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068779.1 Bos taurus surfactant protein B (SFTPB), mRNA.

Price & Availability↑

CloneID	OBa24032
Clone ID Related Accession (Same CDS sequence)	XM_005212760.2 , XM_005212760.3 , NM_001075311.2
Accession Version	NM_001075311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	pulmonary surfactant-associated protein B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC118395.1. On Aug 30, 2012 this sequence version replaced NM_001075311.1. ##Evidence-Data-START## Transcript exon combination :: BC118395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145444, SAMN03145455 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGGGTGTTC TGGCCATGAC TGTGGCCCAG 
GTGTGCCACG TGGTCCCCCT GCTGGTGGGC GGCATCTGCC AGTGCCTGGT TGAGCGCTAC 
TCGGTCATCC TCCTGGACAC GCTGCTAGGC CGCATGCTGC CCCAGCTGGT CTGCGGCCTC 
GTCCTCCGGT GCTCCAGTGA GGACAGCGCT GGCCCAGCCC TCCCTGCCCT GGGGTCCGTG 
CCTGGAGAAT GGCTGCCACA AGACTCTGAC TGCCAGCTCT GCATGTTTGT GACCACCCAG 
GCAGGGAACA GCAGTGAGCA GGCCACGCCA CAGGCAATGC GCCAGGCCTG CCTGGGCACC 
TGGCTGGACA GGCAAAAGTG TGAGCGGTTC GTGGAGGAGA ACGCGCCCCG GCTGCAGACT 
CTGGTGTCCA GTGGCTGGGA TGCCCACATG GCCTGCCAGG CCCTGGGGAC ATGTGCGGCT 
CCGTTCAGTC CTCTCCAGTG TGTCCACAGC CCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068779.1
CDS	21..1142
Translation	MAKSHLLPWLLLLPILCGPGTAAAITYSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVEADDLCQECENISRLLTKMAKEAIFQDSVRKFLEQECDVLPLKLLAPLCRHLLDTYFPLIIEHFQSHMNPKFICQHVGLCKPRHPEPGKGPEPWGPLLDKLALPLLPGVPQAKPGPQTQDLSEQLFPIPIPYCWLCRTLIKRIQAVIPKGVLAMTVAQVCHVVPLLVGGICQCLVERYSVILLDTLLGRMLPQLVCGLVLRCSSEDSAGPALPALGSVPGEWLPQDSDCQLCMFVTTQAGNSSEQATPQAMRQACLGTWLDRQKCERFVEENAPRLQTLVSSGWDAHMACQALGTCAAPFSPLQCVHSPHF

Target ORF information:

RefSeq Version	NM_001075311.2
Organism	Bos taurus(cattle)
Definition	Bos taurus surfactant protein B (SFTPB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075311.2

ORF Insert Sequence:

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGGGTGTTC TGGCCATGAC TGTGGCCCAG 
GTGTGCCACG TGGTCCCCCT GCTGGTGGGC GGCATCTGCC AGTGCCTGGT TGAGCGCTAC 
TCGGTCATCC TCCTGGACAC GCTGCTAGGC CGCATGCTGC CCCAGCTGGT CTGCGGCCTC 
GTCCTCCGGT GCTCCAGTGA GGACAGCGCT GGCCCAGCCC TCCCTGCCCT GGGGTCCGTG 
CCTGGAGAAT GGCTGCCACA AGACTCTGAC TGCCAGCTCT GCATGTTTGT GACCACCCAG 
GCAGGGAACA GCAGTGAGCA GGCCACGCCA CAGGCAATGC GCCAGGCCTG CCTGGGCACC 
TGGCTGGACA GGCAAAAGTG TGAGCGGTTC GTGGAGGAGA ACGCGCCCCG GCTGCAGACT 
CTGGTGTCCA GTGGCTGGGA TGCCCACATG GCCTGCCAGG CCCTGGGGAC ATGTGCGGCT 
CCGTTCAGTC CTCTCCAGTG TGTCCACAGC CCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFTPB ( XM_005212760.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212817.1 Bos taurus surfactant protein B (SFTPB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa24032
Clone ID Related Accession (Same CDS sequence)	XM_005212760.2 , XM_005212760.3 , NM_001075311.2
Accession Version	XM_005212760.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pulmonary surfactant-associated protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104294.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005212760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGGGTGTTC TGGCCATGAC TGTGGCCCAG 
GTGTGCCACG TGGTCCCCCT GCTGGTGGGC GGCATCTGCC AGTGCCTGGT TGAGCGCTAC 
TCGGTCATCC TCCTGGACAC GCTGCTAGGC CGCATGCTGC CCCAGCTGGT CTGCGGCCTC 
GTCCTCCGGT GCTCCAGTGA GGACAGCGCT GGCCCAGCCC TCCCTGCCCT GGGGTCCGTG 
CCTGGAGAAT GGCTGCCACA AGACTCTGAC TGCCAGCTCT GCATGTTTGT GACCACCCAG 
GCAGGGAACA GCAGTGAGCA GGCCACGCCA CAGGCAATGC GCCAGGCCTG CCTGGGCACC 
TGGCTGGACA GGCAAAAGTG TGAGCGGTTC GTGGAGGAGA ACGCGCCCCG GCTGCAGACT 
CTGGTGTCCA GTGGCTGGGA TGCCCACATG GCCTGCCAGG CCCTGGGGAC ATGTGCGGCT 
CCGTTCAGTC CTCTCCAGTG TGTCCACAGC CCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212817.1
CDS	27..1148
Translation	MAKSHLLPWLLLLPILCGPGTAAAITYSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVEADDLCQECENISRLLTKMAKEAIFQDSVRKFLEQECDVLPLKLLAPLCRHLLDTYFPLIIEHFQSHMNPKFICQHVGLCKPRHPEPGKGPEPWGPLLDKLALPLLPGVPQAKPGPQTQDLSEQLFPIPIPYCWLCRTLIKRIQAVIPKGVLAMTVAQVCHVVPLLVGGICQCLVERYSVILLDTLLGRMLPQLVCGLVLRCSSEDSAGPALPALGSVPGEWLPQDSDCQLCMFVTTQAGNSSEQATPQAMRQACLGTWLDRQKCERFVEENAPRLQTLVSSGWDAHMACQALGTCAAPFSPLQCVHSPHF

Target ORF information:

RefSeq Version	XM_005212760.2
Organism	Bos taurus(cattle)
Definition	Bos taurus surfactant protein B (SFTPB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212760.2

ORF Insert Sequence:

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGGGTGTTC TGGCCATGAC TGTGGCCCAG 
GTGTGCCACG TGGTCCCCCT GCTGGTGGGC GGCATCTGCC AGTGCCTGGT TGAGCGCTAC 
TCGGTCATCC TCCTGGACAC GCTGCTAGGC CGCATGCTGC CCCAGCTGGT CTGCGGCCTC 
GTCCTCCGGT GCTCCAGTGA GGACAGCGCT GGCCCAGCCC TCCCTGCCCT GGGGTCCGTG 
CCTGGAGAAT GGCTGCCACA AGACTCTGAC TGCCAGCTCT GCATGTTTGT GACCACCCAG 
GCAGGGAACA GCAGTGAGCA GGCCACGCCA CAGGCAATGC GCCAGGCCTG CCTGGGCACC 
TGGCTGGACA GGCAAAAGTG TGAGCGGTTC GTGGAGGAGA ACGCGCCCCG GCTGCAGACT 
CTGGTGTCCA GTGGCTGGGA TGCCCACATG GCCTGCCAGG CCCTGGGGAC ATGTGCGGCT 
CCGTTCAGTC CTCTCCAGTG TGTCCACAGC CCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFTPB ( XM_005212760.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212817.1 Bos taurus surfactant protein B (SFTPB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa24032
Clone ID Related Accession (Same CDS sequence)	XM_005212760.2 , XM_005212760.3 , NM_001075311.2
Accession Version	XM_005212760.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pulmonary surfactant-associated protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212760.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGGGTGTTC TGGCCATGAC TGTGGCCCAG 
GTGTGCCACG TGGTCCCCCT GCTGGTGGGC GGCATCTGCC AGTGCCTGGT TGAGCGCTAC 
TCGGTCATCC TCCTGGACAC GCTGCTAGGC CGCATGCTGC CCCAGCTGGT CTGCGGCCTC 
GTCCTCCGGT GCTCCAGTGA GGACAGCGCT GGCCCAGCCC TCCCTGCCCT GGGGTCCGTG 
CCTGGAGAAT GGCTGCCACA AGACTCTGAC TGCCAGCTCT GCATGTTTGT GACCACCCAG 
GCAGGGAACA GCAGTGAGCA GGCCACGCCA CAGGCAATGC GCCAGGCCTG CCTGGGCACC 
TGGCTGGACA GGCAAAAGTG TGAGCGGTTC GTGGAGGAGA ACGCGCCCCG GCTGCAGACT 
CTGGTGTCCA GTGGCTGGGA TGCCCACATG GCCTGCCAGG CCCTGGGGAC ATGTGCGGCT 
CCGTTCAGTC CTCTCCAGTG TGTCCACAGC CCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212817.1
CDS	25..1146
Translation	MAKSHLLPWLLLLPILCGPGTAAAITYSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVEADDLCQECENISRLLTKMAKEAIFQDSVRKFLEQECDVLPLKLLAPLCRHLLDTYFPLIIEHFQSHMNPKFICQHVGLCKPRHPEPGKGPEPWGPLLDKLALPLLPGVPQAKPGPQTQDLSEQLFPIPIPYCWLCRTLIKRIQAVIPKGVLAMTVAQVCHVVPLLVGGICQCLVERYSVILLDTLLGRMLPQLVCGLVLRCSSEDSAGPALPALGSVPGEWLPQDSDCQLCMFVTTQAGNSSEQATPQAMRQACLGTWLDRQKCERFVEENAPRLQTLVSSGWDAHMACQALGTCAAPFSPLQCVHSPHF

Target ORF information:

RefSeq Version	XM_005212760.3
Organism	Bos taurus(cattle)
Definition	Bos taurus surfactant protein B (SFTPB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212760.3

ORF Insert Sequence:

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGGGTGTTC TGGCCATGAC TGTGGCCCAG 
GTGTGCCACG TGGTCCCCCT GCTGGTGGGC GGCATCTGCC AGTGCCTGGT TGAGCGCTAC 
TCGGTCATCC TCCTGGACAC GCTGCTAGGC CGCATGCTGC CCCAGCTGGT CTGCGGCCTC 
GTCCTCCGGT GCTCCAGTGA GGACAGCGCT GGCCCAGCCC TCCCTGCCCT GGGGTCCGTG 
CCTGGAGAAT GGCTGCCACA AGACTCTGAC TGCCAGCTCT GCATGTTTGT GACCACCCAG 
GCAGGGAACA GCAGTGAGCA GGCCACGCCA CAGGCAATGC GCCAGGCCTG CCTGGGCACC 
TGGCTGGACA GGCAAAAGTG TGAGCGGTTC GTGGAGGAGA ACGCGCCCCG GCTGCAGACT 
CTGGTGTCCA GTGGCTGGGA TGCCCACATG GCCTGCCAGG CCCTGGGGAC ATGTGCGGCT 
CCGTTCAGTC CTCTCCAGTG TGTCCACAGC CCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFTPB ( XM_015473479.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328965.1 Bos taurus surfactant protein B (SFTPB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa91888
Clone ID Related Accession (Same CDS sequence)	XM_015473479.1 , XM_024998422.1 , XM_015473479.2
Accession Version	XM_015473479.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pulmonary surfactant-associated protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104294.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGTGCCTGG TTGAGCGCTA CTCGGTCATC 
CTCCTGGACA CGCTGCTAGG CCGCATGCTG CCCCAGCTGG TCTGCGGCCT CGTCCTCCGG 
TGCTCCAGTG AGGACAGCGC TGGCCCAGCC CTCCCTGCCC TGGGGTCCGT GCCTGGAGAA 
TGGCTGCCAC AAGACTCTGA CTGCCAGCTC TGCATGTTTG TGACCACCCA GGCAGGGAAC 
AGCAGTGAGC AGGCCACGCC ACAGGCAATG CGCCAGGCCT GCCTGGGCAC CTGGCTGGAC 
AGGCAAAAGT GTGAGCGGTT CGTGGAGGAG AACGCGCCCC GGCTGCAGAC TCTGGTGTCC 
AGTGGCTGGG ATGCCCACAT GGCCTGCCAG GCCCTGGGGA CATGTGCGGC TCCGTTCAGT 
CCTCTCCAGT GTGTCCACAG CCCCCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328965.1
CDS	27..1079
Translation	MAKSHLLPWLLLLPILCGPGTAAAITYSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVEADDLCQECENISRLLTKMAKEAIFQDSVRKFLEQECDVLPLKLLAPLCRHLLDTYFPLIIEHFQSHMNPKFICQHVGLCKPRHPEPGKGPEPWGPLLDKLALPLLPGVPQAKPGPQTQDLSEQLFPIPIPYCWLCRTLIKRIQAVIPKCLVERYSVILLDTLLGRMLPQLVCGLVLRCSSEDSAGPALPALGSVPGEWLPQDSDCQLCMFVTTQAGNSSEQATPQAMRQACLGTWLDRQKCERFVEENAPRLQTLVSSGWDAHMACQALGTCAAPFSPLQCVHSPHF

Target ORF information:

RefSeq Version	XM_015473479.1
Organism	Bos taurus(cattle)
Definition	Bos taurus surfactant protein B (SFTPB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473479.1

ORF Insert Sequence:

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGTGCCTGG TTGAGCGCTA CTCGGTCATC 
CTCCTGGACA CGCTGCTAGG CCGCATGCTG CCCCAGCTGG TCTGCGGCCT CGTCCTCCGG 
TGCTCCAGTG AGGACAGCGC TGGCCCAGCC CTCCCTGCCC TGGGGTCCGT GCCTGGAGAA 
TGGCTGCCAC AAGACTCTGA CTGCCAGCTC TGCATGTTTG TGACCACCCA GGCAGGGAAC 
AGCAGTGAGC AGGCCACGCC ACAGGCAATG CGCCAGGCCT GCCTGGGCAC CTGGCTGGAC 
AGGCAAAAGT GTGAGCGGTT CGTGGAGGAG AACGCGCCCC GGCTGCAGAC TCTGGTGTCC 
AGTGGCTGGG ATGCCCACAT GGCCTGCCAG GCCCTGGGGA CATGTGCGGC TCCGTTCAGT 
CCTCTCCAGT GTGTCCACAG CCCCCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFTPB ( XM_024998422.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854190.1 Bos taurus surfactant protein B (SFTPB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa91888
Clone ID Related Accession (Same CDS sequence)	XM_015473479.1 , XM_024998422.1 , XM_015473479.2
Accession Version	XM_024998422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pulmonary surfactant-associated protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGTGCCTGG TTGAGCGCTA CTCGGTCATC 
CTCCTGGACA CGCTGCTAGG CCGCATGCTG CCCCAGCTGG TCTGCGGCCT CGTCCTCCGG 
TGCTCCAGTG AGGACAGCGC TGGCCCAGCC CTCCCTGCCC TGGGGTCCGT GCCTGGAGAA 
TGGCTGCCAC AAGACTCTGA CTGCCAGCTC TGCATGTTTG TGACCACCCA GGCAGGGAAC 
AGCAGTGAGC AGGCCACGCC ACAGGCAATG CGCCAGGCCT GCCTGGGCAC CTGGCTGGAC 
AGGCAAAAGT GTGAGCGGTT CGTGGAGGAG AACGCGCCCC GGCTGCAGAC TCTGGTGTCC 
AGTGGCTGGG ATGCCCACAT GGCCTGCCAG GCCCTGGGGA CATGTGCGGC TCCGTTCAGT 
CCTCTCCAGT GTGTCCACAG CCCCCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854190.1
CDS	22..1074
Translation	MAKSHLLPWLLLLPILCGPGTAAAITYSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVEADDLCQECENISRLLTKMAKEAIFQDSVRKFLEQECDVLPLKLLAPLCRHLLDTYFPLIIEHFQSHMNPKFICQHVGLCKPRHPEPGKGPEPWGPLLDKLALPLLPGVPQAKPGPQTQDLSEQLFPIPIPYCWLCRTLIKRIQAVIPKCLVERYSVILLDTLLGRMLPQLVCGLVLRCSSEDSAGPALPALGSVPGEWLPQDSDCQLCMFVTTQAGNSSEQATPQAMRQACLGTWLDRQKCERFVEENAPRLQTLVSSGWDAHMACQALGTCAAPFSPLQCVHSPHF

Target ORF information:

RefSeq Version	XM_024998422.1
Organism	Bos taurus(cattle)
Definition	Bos taurus surfactant protein B (SFTPB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998422.1

ORF Insert Sequence:

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGTGCCTGG TTGAGCGCTA CTCGGTCATC 
CTCCTGGACA CGCTGCTAGG CCGCATGCTG CCCCAGCTGG TCTGCGGCCT CGTCCTCCGG 
TGCTCCAGTG AGGACAGCGC TGGCCCAGCC CTCCCTGCCC TGGGGTCCGT GCCTGGAGAA 
TGGCTGCCAC AAGACTCTGA CTGCCAGCTC TGCATGTTTG TGACCACCCA GGCAGGGAAC 
AGCAGTGAGC AGGCCACGCC ACAGGCAATG CGCCAGGCCT GCCTGGGCAC CTGGCTGGAC 
AGGCAAAAGT GTGAGCGGTT CGTGGAGGAG AACGCGCCCC GGCTGCAGAC TCTGGTGTCC 
AGTGGCTGGG ATGCCCACAT GGCCTGCCAG GCCCTGGGGA CATGTGCGGC TCCGTTCAGT 
CCTCTCCAGT GTGTCCACAG CCCCCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFTPB ( XM_015473479.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328965.1 Bos taurus surfactant protein B (SFTPB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91888
Clone ID Related Accession (Same CDS sequence)	XM_015473479.1 , XM_024998422.1 , XM_015473479.2
Accession Version	XM_015473479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pulmonary surfactant-associated protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGTGCCTGG TTGAGCGCTA CTCGGTCATC 
CTCCTGGACA CGCTGCTAGG CCGCATGCTG CCCCAGCTGG TCTGCGGCCT CGTCCTCCGG 
TGCTCCAGTG AGGACAGCGC TGGCCCAGCC CTCCCTGCCC TGGGGTCCGT GCCTGGAGAA 
TGGCTGCCAC AAGACTCTGA CTGCCAGCTC TGCATGTTTG TGACCACCCA GGCAGGGAAC 
AGCAGTGAGC AGGCCACGCC ACAGGCAATG CGCCAGGCCT GCCTGGGCAC CTGGCTGGAC 
AGGCAAAAGT GTGAGCGGTT CGTGGAGGAG AACGCGCCCC GGCTGCAGAC TCTGGTGTCC 
AGTGGCTGGG ATGCCCACAT GGCCTGCCAG GCCCTGGGGA CATGTGCGGC TCCGTTCAGT 
CCTCTCCAGT GTGTCCACAG CCCCCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328965.1
CDS	22..1074
Translation	MAKSHLLPWLLLLPILCGPGTAAAITYSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVEADDLCQECENISRLLTKMAKEAIFQDSVRKFLEQECDVLPLKLLAPLCRHLLDTYFPLIIEHFQSHMNPKFICQHVGLCKPRHPEPGKGPEPWGPLLDKLALPLLPGVPQAKPGPQTQDLSEQLFPIPIPYCWLCRTLIKRIQAVIPKCLVERYSVILLDTLLGRMLPQLVCGLVLRCSSEDSAGPALPALGSVPGEWLPQDSDCQLCMFVTTQAGNSSEQATPQAMRQACLGTWLDRQKCERFVEENAPRLQTLVSSGWDAHMACQALGTCAAPFSPLQCVHSPHF

Target ORF information:

RefSeq Version	XM_015473479.2
Organism	Bos taurus(cattle)
Definition	Bos taurus surfactant protein B (SFTPB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473479.2

ORF Insert Sequence:

ATGGCCAAGT CACACCTGCT TCCATGGCTT CTGCTGCTGC CCATACTCTG TGGTCCGGGC 
ACTGCTGCTG CGATCACCTA TTCCCTGGCC TGTGCCCAGG GCCCCGAGTT CTGGTGTCAA 
AGTCTGGAGC AAGCATTGCA GTGCAGAGCC CTAGGGCACT GCCTGCAGGA AGTCTGGGGA 
CATGTGGAAG CCGATGACCT GTGCCAGGAA TGTGAGAACA TCTCCCGCCT CCTCACCAAG 
ATGGCCAAGG AGGCCATTTT CCAGGACTCA GTGCGCAAAT TTCTGGAGCA GGAGTGCGAT 
GTCCTTCCGC TGAAACTGTT GGCGCCCCTG TGTCGCCACC TGCTGGACAC CTATTTCCCT 
CTGATCATTG AGCACTTCCA GAGCCATATG AACCCGAAGT TCATCTGTCA GCACGTGGGC 
CTATGCAAGC CCAGGCACCC AGAGCCAGGG AAGGGGCCAG AGCCATGGGG CCCTCTGCTG 
GACAAGCTGG CCCTCCCCCT GCTGCCAGGG GTCCCCCAGG CCAAGCCTGG GCCTCAGACA 
CAGGACCTCT CTGAGCAGCT GTTCCCCATT CCCATCCCCT ACTGCTGGCT CTGCCGGACT 
CTGATCAAAC GGATCCAGGC TGTGATTCCC AAGTGCCTGG TTGAGCGCTA CTCGGTCATC 
CTCCTGGACA CGCTGCTAGG CCGCATGCTG CCCCAGCTGG TCTGCGGCCT CGTCCTCCGG 
TGCTCCAGTG AGGACAGCGC TGGCCCAGCC CTCCCTGCCC TGGGGTCCGT GCCTGGAGAA 
TGGCTGCCAC AAGACTCTGA CTGCCAGCTC TGCATGTTTG TGACCACCCA GGCAGGGAAC 
AGCAGTGAGC AGGCCACGCC ACAGGCAATG CGCCAGGCCT GCCTGGGCAC CTGGCTGGAC 
AGGCAAAAGT GTGAGCGGTT CGTGGAGGAG AACGCGCCCC GGCTGCAGAC TCTGGTGTCC 
AGTGGCTGGG ATGCCCACAT GGCCTGCCAG GCCCTGGGGA CATGTGCGGC TCCGTTCAGT 
CCTCTCCAGT GTGTCCACAG CCCCCACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Orientation and depth of surfactant protein B C-terminal helix in lung surfactant bilayers.
Biochimica et biophysica acta1818(5)1165-72(2012 May)
Bertani P,Vidovic V,Yang TC,Rendell J,Gordon LM,Waring AJ,Bechinger B,Booth V

Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.
American journal of physiology. Lung cellular and molecular physiology302(2)L257-65(2012 Jan)
Hite RD,Grier BL,Waite BM,Veldhuizen RA,Possmayer F,Yao LJ,Seeds MC

Hydrophobic surfactant proteins induce a phosphatidylethanolamine to form cubic phases.
Biophysical journal98(8)1549-57(2010 Apr)
Chavarha M,Khoojinian H,Schulwitz LE Jr,Biswas SC,Rananavare SB,Hall SB

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Modification of tryptophan and methionine residues is implicated in the oxidative inactivation of surfactant protein B.
Biochemistry46(18)5604-15(2007 May)
Manzanares D,Rodriguez-Capote K,Liu S,Haines T,Ramos Y,Zhao L,Doherty-Kirby A,Lajoie G,Possmayer F

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SFTPB cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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