GNL2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNL2
Entrez Gene ID	507369
Full Name	G protein nucleolar 2
General protein information	Preferred Names G protein nucleolar 2 Names nucleolar GTP-binding protein 2 guanine nucleotide binding protein-like 2 (nucleolar)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986789.1	XP_024842557.1	nucleolar GTP-binding protein 2 isoform X1
NM_001045967.2	NP_001039432.1	nucleolar GTP-binding protein 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gnl2	NP_001020907.1
Gallus gallus (chicken)	GNL2	XP_417761.3
Drosophila melanogaster (fruit fly)	ns2	NP_611232.1
Caenorhabditis elegans (roundworm)	ngp-1	NP_001021621.1
Saccharomyces cerevisiae (baker's yeast)	NOG2	NP_014451.1
Homo sapiens (human)	GNL2	NP_037417.1
Danio rerio (zebrafish)	gnl2	NP_998389.1
Schizosaccharomyces pombe (fission yeast)	SPAC6F6.03c	NP_593896.1
Neurospora crassa	NCU02546	XP_965677.1
Canis lupus familiaris (dog)	GNL2	XP_532556.2
Mus musculus (house mouse)	Gnl2	NP_663527.2
Danio rerio (zebrafish)	LOC100536859	XP_003200256.1
Eremothecium gossypii	AGOS_ABR120C	NP_983067.1
Magnaporthe oryzae (rice blast fungus)	MGG_01561	XP_003714536.1
Oryza sativa (rice)	Os03g0352400	NP_001050123.1
Pan troglodytes (chimpanzee)	GNL2	NP_001233327.1
	GNL2	XP_001111847.2
Bos taurus (cattle)	GNL2	NP_001039432.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011309	XP_309340.4
Kluyveromyces lactis	KLLA0C18843g	XP_453040.1
Arabidopsis thaliana (thale cress)	AT1G52980	NP_175706.1
Xenopus tropicalis (tropical clawed frog)	gnl2	NP_001001243.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa38886	NM_001045967.2 Latest version!	Bos taurus G protein nucleolar 2 (GNL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa232615	XM_024986789.1 Latest version!	Bos taurus G protein nucleolar 2 (GNL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNL2 ( NM_001045967.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039432.1 Bos taurus G protein nucleolar 2 (GNL2), mRNA.

Price & Availability↑

CloneID	OBa38886
Clone ID Related Accession (Same CDS sequence)	NM_001045967.2
Accession Version	NM_001045967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	nucleolar GTP-binding protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC110174.1. On Aug 30, 2012 this sequence version replaced NM_001045967.1. ##Evidence-Data-START## Transcript exon combination :: BC110174.1, BT025485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAAGC CCAAGTACAA AGGACGGAGC ACTATCAACC CCTCTAATGC CAGCACAAAT 
CCCGATCGAG TGCAGGGAGC AGGAGGTCAC AACATGAGGG ACCGGGCCAC AATTCGACGC 
CTGAATATGT ACAGACAGAA GGAGCGCAGG AACAGCCGTG GTAAAGTGAT TAAGCCTCTG 
CAGTATCAGT CAACAGTGGC TTCTGGCACA GTGGCAAGAG TGGAGCCAAA CATTAAGTGG 
TTTGGAAATA CACGTGTGAT TAAGCAGTCA TCATTACAAA AATTTCAAGA GGAAATGGAC 
ACAGTCTTGA AGGATCCATA CAAAGTTGTC ATGAAGCAAA GCAAGTTACC CATGTCTCTT 
CTCCATGATC GAATCCGGCC TCATAATGCA AAGGTGCACA TCCTTGATAC TGAAAGCTTC 
GAAACAACAT TTGGCCCCAA AGCGCAGAGG AAGCGGCCAA ATTTATTTGC AAGTGATATG 
CAGTCACTTA TAGAAAATGC AGAAACATCC ACTGAGAGCT ATGACCAGGG CAAGGATCGT 
GACTTGGTAG CTGAAGACAC TGGTGTGAGA AATGAAGCCC AAGAAGAGAT ATTTAAAAAG 
GGACAGTCTA AAAGAATATG GGGTGAGCTC TACAAGGTGA TAGATTCATC AGATGTTGTG 
GTTCAAGTTC TTGATGCTAG AGATCCAGTG GGTACACGTT CCCCTCACAT TGAGACTTAC 
CTGAAAAAAG AGAAACCCTG GAAACATCTC ATTTTTGTTC TTAACAAATG TGACCTTGTT 
CCAACCTGGG CAACGAAACG ATGGGTTGCC GTCCTCTCCC AGGATTACCC GACGCTTGCT 
TTCCACGCGA GTCTTACTAA CCCCTTTGGC AAAGGAGCGT TTATTCAGCT TCTGCGGCAG 
TTTGGAAAGT TGCACACTGA CAAGAAGCAG ATCAGCGTGG GGTTCATTGG CTATCCAAAC 
GTTGGCAAGA GCTCTGTGAT AAACACACTG CGCTCCAAGA AGGTCTGCAA CGTGGCCCCC 
ATCGCAGGTG AAACAAAGGT CTGGCAGTAC ATTACCTTGA TGCGTCGGAT CTTCCTCATC 
GACTGTCCGG GTGTGGTTTA CCCCTCCGAG GACTCAGAGA CGGACATTGT GCTGAAGGGA 
GTCGTTCAAG TTGAAAAAAT TAAGACTCCT GAAGACCACA TTGGTGCTGT ACTTGAACGA 
GCAAAACCAG AGTATATCAG CAAGACGTAC AAGATTAATT CTTGGGAGAA CGCTGAGGAC 
TTTCTGGAGA AGCTAGCTTT CCGGACAGGG AAGTTATTAA AGGGTGGAGA GCCCAACCTG 
CGGACTGTGG GGAAGATGGT CCTCAGTGAC TGGCAGAGGG GCCGGATTCC TTTCTTTGTC 
AAGCCGCCCA ATGCAGAACC CCCAGCAGCC CCCGAGCTTC CATCCTCCTC ATCTTTGGAA 
GTTGCCGCAG AAACAACCCA GAACAACCCA GGAGAGAAAG TCACGGAAAC AGTAGGTGAG 
GAGTCGGAAT CCATCCTCGA GAAGGAAAAA GAGGAGCCCC GGCACTGTGG CATCGATTCA 
GAAATGCAGC AGATTCTGGC GCGGGTCCGG CAGAACTTTG GCAAGATCAA CGTTGCACCT 
CAGTTTTCTG GGGATGACCT GGTTCCTCTG GAGATGTCCG ATATCGATGA CGAGCTGGAG 
AGCCTTTCTG CGGAGAATGA GGAGGAGGAG GAACAGGAGT ACCCAGGAGA CGATGAGGAC 
GAGTCTTATG CAGAGTCCCA GGAGGAACAT GCGGGACGCA CCACTAAGGC CGTTATTCAA 
GCCCTGGATG AAAAGATCGC CAAGTACCAG AGGTTTTTAA ACAAAGCCAA AGCTAAGAAG 
TTTTCAGCAA TCAGGATATC CAAGGGACTG AGTGAAATGG TTTTTGCAAA ATCCGAAGGA 
CGAAAAAAAG GAAAAGCTGA AGAAGATGTA GATGATACAG CACCTCCCAA AAAGGGAAAG 
AAGAGGAAGG CCCAGAGGGA AGAGGAGCAG CCAGACAAAA CCCGCAGGAC GCTTACGTCT 
AAGGAGAGGA GGCGAGAAGC ACGGCAGCAG CGATCTAGAA AAGTCGGCAT ACGCTACTAT 
GAAACACACA ATGTGAAGAA TAGGAACAGG AACAAAAAGA AGACCACCGA CTCAGAAGGG 
CAGAAACACA GACACAAGAA AGCCAAACAT AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039432.1
CDS	77..2275
Translation	MVKPKYKGRSTINPSNASTNPDRVQGAGGHNMRDRATIRRLNMYRQKERRNSRGKVIKPLQYQSTVASGTVARVEPNIKWFGNTRVIKQSSLQKFQEEMDTVLKDPYKVVMKQSKLPMSLLHDRIRPHNAKVHILDTESFETTFGPKAQRKRPNLFASDMQSLIENAETSTESYDQGKDRDLVAEDTGVRNEAQEEIFKKGQSKRIWGELYKVIDSSDVVVQVLDARDPVGTRSPHIETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQLLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRIFLIDCPGVVYPSEDSETDIVLKGVVQVEKIKTPEDHIGAVLERAKPEYISKTYKINSWENAEDFLEKLAFRTGKLLKGGEPNLRTVGKMVLSDWQRGRIPFFVKPPNAEPPAAPELPSSSSLEVAAETTQNNPGEKVTETVGEESESILEKEKEEPRHCGIDSEMQQILARVRQNFGKINVAPQFSGDDLVPLEMSDIDDELESLSAENEEEEEQEYPGDDEDESYAESQEEHAGRTTKAVIQALDEKIAKYQRFLNKAKAKKFSAIRISKGLSEMVFAKSEGRKKGKAEEDVDDTAPPKKGKKRKAQREEEQPDKTRRTLTSKERRREARQQRSRKVGIRYYETHNVKNRNRNKKKTTDSEGQKHRHKKAKHKQ

Target ORF information:

RefSeq Version	NM_001045967.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein nucleolar 2 (GNL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045967.2

ORF Insert Sequence:

ATGGTGAAGC CCAAGTACAA AGGACGGAGC ACTATCAACC CCTCTAATGC CAGCACAAAT 
CCCGATCGAG TGCAGGGAGC AGGAGGTCAC AACATGAGGG ACCGGGCCAC AATTCGACGC 
CTGAATATGT ACAGACAGAA GGAGCGCAGG AACAGCCGTG GTAAAGTGAT TAAGCCTCTG 
CAGTATCAGT CAACAGTGGC TTCTGGCACA GTGGCAAGAG TGGAGCCAAA CATTAAGTGG 
TTTGGAAATA CACGTGTGAT TAAGCAGTCA TCATTACAAA AATTTCAAGA GGAAATGGAC 
ACAGTCTTGA AGGATCCATA CAAAGTTGTC ATGAAGCAAA GCAAGTTACC CATGTCTCTT 
CTCCATGATC GAATCCGGCC TCATAATGCA AAGGTGCACA TCCTTGATAC TGAAAGCTTC 
GAAACAACAT TTGGCCCCAA AGCGCAGAGG AAGCGGCCAA ATTTATTTGC AAGTGATATG 
CAGTCACTTA TAGAAAATGC AGAAACATCC ACTGAGAGCT ATGACCAGGG CAAGGATCGT 
GACTTGGTAG CTGAAGACAC TGGTGTGAGA AATGAAGCCC AAGAAGAGAT ATTTAAAAAG 
GGACAGTCTA AAAGAATATG GGGTGAGCTC TACAAGGTGA TAGATTCATC AGATGTTGTG 
GTTCAAGTTC TTGATGCTAG AGATCCAGTG GGTACACGTT CCCCTCACAT TGAGACTTAC 
CTGAAAAAAG AGAAACCCTG GAAACATCTC ATTTTTGTTC TTAACAAATG TGACCTTGTT 
CCAACCTGGG CAACGAAACG ATGGGTTGCC GTCCTCTCCC AGGATTACCC GACGCTTGCT 
TTCCACGCGA GTCTTACTAA CCCCTTTGGC AAAGGAGCGT TTATTCAGCT TCTGCGGCAG 
TTTGGAAAGT TGCACACTGA CAAGAAGCAG ATCAGCGTGG GGTTCATTGG CTATCCAAAC 
GTTGGCAAGA GCTCTGTGAT AAACACACTG CGCTCCAAGA AGGTCTGCAA CGTGGCCCCC 
ATCGCAGGTG AAACAAAGGT CTGGCAGTAC ATTACCTTGA TGCGTCGGAT CTTCCTCATC 
GACTGTCCGG GTGTGGTTTA CCCCTCCGAG GACTCAGAGA CGGACATTGT GCTGAAGGGA 
GTCGTTCAAG TTGAAAAAAT TAAGACTCCT GAAGACCACA TTGGTGCTGT ACTTGAACGA 
GCAAAACCAG AGTATATCAG CAAGACGTAC AAGATTAATT CTTGGGAGAA CGCTGAGGAC 
TTTCTGGAGA AGCTAGCTTT CCGGACAGGG AAGTTATTAA AGGGTGGAGA GCCCAACCTG 
CGGACTGTGG GGAAGATGGT CCTCAGTGAC TGGCAGAGGG GCCGGATTCC TTTCTTTGTC 
AAGCCGCCCA ATGCAGAACC CCCAGCAGCC CCCGAGCTTC CATCCTCCTC ATCTTTGGAA 
GTTGCCGCAG AAACAACCCA GAACAACCCA GGAGAGAAAG TCACGGAAAC AGTAGGTGAG 
GAGTCGGAAT CCATCCTCGA GAAGGAAAAA GAGGAGCCCC GGCACTGTGG CATCGATTCA 
GAAATGCAGC AGATTCTGGC GCGGGTCCGG CAGAACTTTG GCAAGATCAA CGTTGCACCT 
CAGTTTTCTG GGGATGACCT GGTTCCTCTG GAGATGTCCG ATATCGATGA CGAGCTGGAG 
AGCCTTTCTG CGGAGAATGA GGAGGAGGAG GAACAGGAGT ACCCAGGAGA CGATGAGGAC 
GAGTCTTATG CAGAGTCCCA GGAGGAACAT GCGGGACGCA CCACTAAGGC CGTTATTCAA 
GCCCTGGATG AAAAGATCGC CAAGTACCAG AGGTTTTTAA ACAAAGCCAA AGCTAAGAAG 
TTTTCAGCAA TCAGGATATC CAAGGGACTG AGTGAAATGG TTTTTGCAAA ATCCGAAGGA 
CGAAAAAAAG GAAAAGCTGA AGAAGATGTA GATGATACAG CACCTCCCAA AAAGGGAAAG 
AAGAGGAAGG CCCAGAGGGA AGAGGAGCAG CCAGACAAAA CCCGCAGGAC GCTTACGTCT 
AAGGAGAGGA GGCGAGAAGC ACGGCAGCAG CGATCTAGAA AAGTCGGCAT ACGCTACTAT 
GAAACACACA ATGTGAAGAA TAGGAACAGG AACAAAAAGA AGACCACCGA CTCAGAAGGG 
CAGAAACACA GACACAAGAA AGCCAAACAT AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNL2 ( XM_024986789.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842557.1 Bos taurus G protein nucleolar 2 (GNL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232615
Clone ID Related Accession (Same CDS sequence)	XM_024986789.1
Accession Version	XM_024986789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nucleolar GTP-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCAC TTATAGAAAA TGCAGAAACA TCCACTGAGA GCTATGACCA GGGCAAGGAT 
CGTGACTTGG TAGCTGAAGA CACTGGTGTG AGAAATGAAG CCCAAGAAGA GATATTTAAA 
AAGGGACAGT CTAAAAGAAT ATGGGGTGAG CTCTACAAGG TGATAGATTC ATCAGATGTT 
GTGGTTCAAG TTCTTGATGC TAGAGATCCA GTGGGTACAC GTTCCCCTCA CATTGAGACT 
TACCTGAAAA AAGAGAAACC CTGGAAACAT CTCATTTTTG TTCTTAACAA ATGTGACCTT 
GTTCCAACCT GGGCAACGAA ACGATGGGTT GCCGTCCTCT CCCAGGATTA CCCGACGCTT 
GCTTTCCACG CGAGTCTTAC TAACCCCTTT GGCAAAGGAG CGTTTATTCA GCTTCTGCGG 
CAGTTTGGAA AGTTGCACAC TGACAAGAAG CAGATCAGCG TGGGGTTCAT TGGCTATCCA 
AACGTTGGCA AGAGCTCTGT GATAAACACA CTGCGCTCCA AGAAGGTCTG CAACGTGGCC 
CCCATCGCAG GTGAAACAAA GGTCTGGCAG TACATTACCT TGATGCGTCG GATCTTCCTC 
ATCGACTGTC CGGGTGTGGT TTACCCCTCC GAGGACTCAG AGACGGACAT TGTGCTGAAG 
GGAGTCGTTC AAGTTGAAAA AATTAAGACT CCTGAAGACC ACATTGGTGC TGTACTTGAA 
CGAGCAAAAC CAGAGTATAT CAGCAAGACG TACAAGATTA ATTCTTGGGA GAACGCTGAG 
GACTTTCTGG AGAAGCTAGC TTTCCGGACA GGGAAGTTAT TAAAGGGTGG AGAGCCCAAC 
CTGCGGACTG TGGGGAAGAT GGTCCTCAAT GACTGGCAGA GGGGCCGGAT TCCTTTCTTT 
GTCAAGCCGC CCAATGCAGA ACCCCCAGCA GCCCCCGAGC TTCCATCCTC CTCATCTTTG 
GAAGTTGCCG CAGAAACAAC CCAGAACAAC CCAGGAGAGA AAGTCACGGA AACAGTAGGT 
GAGGAGTCGG AATCCATCCT CGAGAAGGAA AAAGAGGAGC CCCGGCACTG TGGCATCGAT 
TCAGAAATGC AGCAGATTCT GGCGCGGGTC CGGCAGAACT TTGGCAAGAT CAACGTTGCA 
CCTCAGTTTT CTGGGGATGA CCTGGTTCCT CTGGAGATGT CCGATATCGA TGACGAGCTG 
GAGAGCCTTT CTGCGGAGAA TGAGGAGGAG GAGGAACAGG AGTACCCAGG AGACGATGAG 
GACGAGTCTT ATGCAGAGTC CCAGGAGGAA CATGCGGGAC GCACCACTAA GGCCGTTATT 
CAAGCCCTGG ATGAAAAGAT CGCCAAGTAC CAGAGGTTTT TAAACAAAGC CAAAGCTAAG 
AAGTTTTCAG CAATCAGGAT ATCCAAGGGA CTGAGTGAAA TGGTTTTTGC AAAATCCGAA 
GGACGAAAAA AAGGAAAAGC TGAAGAAGAT GTAGATGATA CAGCACCTCC CAAAAAGGGA 
AAGAAGAGGA AGGCCCAGAG GGAAGAGGAG CAGCCAGACA AAACCCGCAG GACGCTTACG 
TCTAAGGAGA GGAGGCGAGA AGCACGGCAG CAGCGATCTA GAAAAGTCGG CATACGCTAC 
TATGAAACAC ACAATGTGAA GAATAGGAAC AGGAACAAAA AGAAGACCAC CGACTCAGAA 
GGGCAGAAAC ACAGACACAA GAAAGCCAAA CATAAGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842557.1
CDS	189..1910
Translation	MQSLIENAETSTESYDQGKDRDLVAEDTGVRNEAQEEIFKKGQSKRIWGELYKVIDSSDVVVQVLDARDPVGTRSPHIETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQLLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRIFLIDCPGVVYPSEDSETDIVLKGVVQVEKIKTPEDHIGAVLERAKPEYISKTYKINSWENAEDFLEKLAFRTGKLLKGGEPNLRTVGKMVLNDWQRGRIPFFVKPPNAEPPAAPELPSSSSLEVAAETTQNNPGEKVTETVGEESESILEKEKEEPRHCGIDSEMQQILARVRQNFGKINVAPQFSGDDLVPLEMSDIDDELESLSAENEEEEEQEYPGDDEDESYAESQEEHAGRTTKAVIQALDEKIAKYQRFLNKAKAKKFSAIRISKGLSEMVFAKSEGRKKGKAEEDVDDTAPPKKGKKRKAQREEEQPDKTRRTLTSKERRREARQQRSRKVGIRYYETHNVKNRNRNKKKTTDSEGQKHRHKKAKHKQ

Target ORF information:

RefSeq Version	XM_024986789.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein nucleolar 2 (GNL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986789.1

ORF Insert Sequence:

ATGCAGTCAC TTATAGAAAA TGCAGAAACA TCCACTGAGA GCTATGACCA GGGCAAGGAT 
CGTGACTTGG TAGCTGAAGA CACTGGTGTG AGAAATGAAG CCCAAGAAGA GATATTTAAA 
AAGGGACAGT CTAAAAGAAT ATGGGGTGAG CTCTACAAGG TGATAGATTC ATCAGATGTT 
GTGGTTCAAG TTCTTGATGC TAGAGATCCA GTGGGTACAC GTTCCCCTCA CATTGAGACT 
TACCTGAAAA AAGAGAAACC CTGGAAACAT CTCATTTTTG TTCTTAACAA ATGTGACCTT 
GTTCCAACCT GGGCAACGAA ACGATGGGTT GCCGTCCTCT CCCAGGATTA CCCGACGCTT 
GCTTTCCACG CGAGTCTTAC TAACCCCTTT GGCAAAGGAG CGTTTATTCA GCTTCTGCGG 
CAGTTTGGAA AGTTGCACAC TGACAAGAAG CAGATCAGCG TGGGGTTCAT TGGCTATCCA 
AACGTTGGCA AGAGCTCTGT GATAAACACA CTGCGCTCCA AGAAGGTCTG CAACGTGGCC 
CCCATCGCAG GTGAAACAAA GGTCTGGCAG TACATTACCT TGATGCGTCG GATCTTCCTC 
ATCGACTGTC CGGGTGTGGT TTACCCCTCC GAGGACTCAG AGACGGACAT TGTGCTGAAG 
GGAGTCGTTC AAGTTGAAAA AATTAAGACT CCTGAAGACC ACATTGGTGC TGTACTTGAA 
CGAGCAAAAC CAGAGTATAT CAGCAAGACG TACAAGATTA ATTCTTGGGA GAACGCTGAG 
GACTTTCTGG AGAAGCTAGC TTTCCGGACA GGGAAGTTAT TAAAGGGTGG AGAGCCCAAC 
CTGCGGACTG TGGGGAAGAT GGTCCTCAAT GACTGGCAGA GGGGCCGGAT TCCTTTCTTT 
GTCAAGCCGC CCAATGCAGA ACCCCCAGCA GCCCCCGAGC TTCCATCCTC CTCATCTTTG 
GAAGTTGCCG CAGAAACAAC CCAGAACAAC CCAGGAGAGA AAGTCACGGA AACAGTAGGT 
GAGGAGTCGG AATCCATCCT CGAGAAGGAA AAAGAGGAGC CCCGGCACTG TGGCATCGAT 
TCAGAAATGC AGCAGATTCT GGCGCGGGTC CGGCAGAACT TTGGCAAGAT CAACGTTGCA 
CCTCAGTTTT CTGGGGATGA CCTGGTTCCT CTGGAGATGT CCGATATCGA TGACGAGCTG 
GAGAGCCTTT CTGCGGAGAA TGAGGAGGAG GAGGAACAGG AGTACCCAGG AGACGATGAG 
GACGAGTCTT ATGCAGAGTC CCAGGAGGAA CATGCGGGAC GCACCACTAA GGCCGTTATT 
CAAGCCCTGG ATGAAAAGAT CGCCAAGTAC CAGAGGTTTT TAAACAAAGC CAAAGCTAAG 
AAGTTTTCAG CAATCAGGAT ATCCAAGGGA CTGAGTGAAA TGGTTTTTGC AAAATCCGAA 
GGACGAAAAA AAGGAAAAGC TGAAGAAGAT GTAGATGATA CAGCACCTCC CAAAAAGGGA 
AAGAAGAGGA AGGCCCAGAG GGAAGAGGAG CAGCCAGACA AAACCCGCAG GACGCTTACG 
TCTAAGGAGA GGAGGCGAGA AGCACGGCAG CAGCGATCTA GAAAAGTCGG CATACGCTAC 
TATGAAACAC ACAATGTGAA GAATAGGAAC AGGAACAAAA AGAAGACCAC CGACTCAGAA 
GGGCAGAAAC ACAGACACAA GAAAGCCAAA CATAAGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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GNL2 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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