CRYBG2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYBG2
Entrez Gene ID	507142
Full Name	crystallin beta-gamma domain containing 2
Synonyms	AIM1L
General protein information	Preferred Names crystallin beta-gamma domain containing 2 Names absent in melanoma 1-like protein absent in melanoma 1 like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015460082.1	XP_015315568.1	absent in melanoma 1-like protein isoform X1
XM_005203089.3	XP_005203146.1	absent in melanoma 1-like protein isoform X2
NM_001109791.1	NP_001103261.1	beta/gamma crystallin domain-containing protein 2
XM_005203089.4	XP_005203146.1	beta/gamma crystallin domain-containing protein 2 isoform X1
XM_015460083.1	XP_015315569.1	absent in melanoma 1-like protein isoform X3
XM_015460083.2	XP_015315569.1	beta/gamma crystallin domain-containing protein 2 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	AIM1L	NP_001034864.2
Bos taurus (cattle)	LOC507142	NP_001103261.1
Rattus norvegicus (Norway rat)	Aim1l	XP_216539.7
Pan troglodytes (chimpanzee)	AIM1L	XP_513230.3
Canis lupus familiaris (dog)	AIM1L	XP_854991.3
Mus musculus (house mouse)	Aim1l	NP_001156442.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CRYBG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYBG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa88050	XM_015460082.1 Latest version!	Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa88051	XM_015460083.2 Latest version!	Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa36922	NM_001109791.1 Latest version!	Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa16903	XM_005203089.4 Latest version!	Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa16903	XM_005203089.3	Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa88051	XM_015460083.1	Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYBG2 ( XM_015460082.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315568.1 Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X1, mRNA.

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CloneID	OBa88050
Clone ID Related Accession (Same CDS sequence)	XM_015460082.1
Accession Version	XM_015460082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	absent in melanoma 1-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGG AACTTTCCAG AGCTCTTCCT CTTTGTCCAG CTCTTGTGGC CCTGGCAGCC 
TCTCCGGAGG GCAACTCCGC TTGGTGTCAG AGCTCTGCTC AGCTTCTCCG GTGCCAAGGA 
GGGCAGCCAG GGCTGTCGTG GGGTACCCAG GGGGCAGGCC AGAGAGGCTG TCACTGGGTC 
TCTGCTTCTG CTTTCCCTCA GGCCCAAGCC CAGAGCCACT CAGAGTTGGA CCTTCGTGCA 
GGGAGCCTTG GCTCCTGGCT TTGGAAGCGA CGACACAGCT CAGTGGGGCC CGTGGAGCCT 
CTGGGGCGCC TCCAGAGGCC TGCTGTAGGC AGTGCCTCTG ACCTGGCCAA CCATGCCTCC 
ATGGGGCCGG AGAGCCTGGC ACCAGCGGTG ACCTCGGGAG AGCCTGCTGG AGCCAGGGGT 
CCCTCAGGCC CACCGCCGGT GCCTGGATGT TTGGAACAAG GACCTCCGGG CCACACCTGC 
CAGCAACCCC CACACTCAGC CCGGGGGTTT CCTCTGTGGC CCAAGGCACC CAGCAACCTG 
GAAGACGTGC TGCCCCACCC AGCGTCCTGC CCCAGGGCAG AGGGGGCAAA GGGCGAGCCT 
ACCACCTCAG GGCCGGCCCT GCCAGAGCCA ACCCTGCCAG AGCCAGCCCA GACCCTACGT 
GAGCGTCCCT CTGCCAGGAG GACCCGCTAC TACCGCATCA CTGTCAGCCT GCAGGGGCGT 
GAGCAAGCAC CTGGGGAGGA GACTGAAGAG CCCAAACCGG CCCAACCAGC TCCCCACCCC 
TGCGGCCCTG AGGAATCCAG GGGCTGGCAG GAGCCTCCTC AGGGGCCCCG CCCCATCACA 
GGGTGCCTAG CTGACCCGCC TCAGGAACCC CAGCTCCGAG CCCCACCACA TCAGGGGAGC 
ACGGTCCAGC AGCAGGAGCT CCGGGCCGGC CGGACGCCTG GGTCCCCGCA CAGACGCGAG 
CTGGACCACA GCGAGCCAAG GACACCTCAG GACGCCTTGC CAGGGCCCAA CATGGAGGAG 
GCCAGCGGGC CTCCGGCTGA GGCCAAGGCC CGAGTGGTGA GCACCACGCT GACATGGCGG 
CAGCGGCCCC CTGCCCAGGA CGAGGTCAAA CACCGTTTCC ACAAGGTGTC CCTGGTGTCA 
GGGGCCCAGT CGGAAGCCCC GTCCGAGGAG ACGTCTGAGT CCAGCCATCG TCGAGAGGAA 
GTCAATGGCT TTGCTGTCCG GGAGGAAGAG ACCGTGAACC ACCAGGGCCC CCGGGACGGG 
GCTGGCTCCA AGAGCTTCCA GAGCCACGGG CCCATCTTTT CCAAGAAGTA CACATTACCC 
CTCAAGGAGA AAAGGCCAGT GGGAAGACTG AAGGAGGCTG CGGAGCAGGG TGACGGCAGC 
CCCCAGCCCC CCAGGACCGA GCCGCCCAGC GCGGGAGTCA CGGCCAGGAC TGAGTTGTCG 
GTGCCAGCAC ACGGGCCCCG GGAGCCGAGC CCCCACCCGG GTGTGAGTTT CACCAGCGGG 
AGCTCCAGGA GCCTGGAGGA GCGGCGGGTG ACACGCACTG TGCGGACCAC CGTGATGGTG 
GGAGGGCACG TGGACCGCCG GGTGAACAGC ACCGTGATCA ACTCCGTGGG GCCCGCGCTC 
GTGGGCGAGG CCCTGCCAAG GGGCCGCAAT GCTGCCCGCG CGGTGCGGGC AGTGGTGGTG 
AACCCGCGGG CCGAGGGCTC CCCCAGTCGC AGTCAGGCCC TGGAGCTGCT AAGTAGCCTT 
GTGCCCACTG AGCGGACCGC CCCCACCAGC CGGCTTCCCA GGCCCCTGGC TGCTGTGCCA 
AGGAGTCCAG ATCTGGGCAG CACAGAAGGG TCAGCCCTGA GGCAGCTGCC TGAGACGAGG 
ACAGCAGAGC CGAAGGACAG ATCTGCCCCG GGCCCTGCGG GCTTCCTGGA GGAAGCTCAC 
CCCACTCCGA GCCAGGAGGT CCCTGCAGCC CACCCAGACC AGGAACAGGA CAGAACCCTG 
GGTGCCGGGG TCCATGAGGT CCCAAGGGTG CAGAGACCCG CCAGCCCCAC CCAGTCCCCT 
CTCCAGACCT CTCAGGGCCA CACCCCAGTA CCTTCCTCTC CCAGACTCCA GACCCGAACC 
CTCTCCCTGG ACCTAGTTCA ACCCCCAGAG CAACCTGAAG CACCTACTCA GGCTCGGGCC 
CAGCTCACTC TCAAGCCCCA GGGACCCCAG GCCCTGCCCT CCATCAAAAG AGAAGGGCTT 
TCAGATCCCC CTGCGGCCAC CGTCCTTCCT ATGGTGAAGA CTGAGGTTGT GATAGTCCCT 
GGACAATCTC TTGCTCCATC CTCTATGAGA TGGAAGGCCA TCACCAGCCC GGGAGGTCTT 
TCTGCTCCAT CCTCCCCAAA GAATAAGGTT GTTCAGGACT CTGAAAAAGT CCCTGTTGTC 
TCCCTTACCC AGAAAGAGAT AGTACAGGGC CCTGGAGTTC CTGCTGACTC ATCTCCCAAC 
CAAAAAGAGG TAGTGCAGGG TCGGAGTGCT CCTGCTGCCT CAGGCCCCAA ACCAACCAAC 
GTTGCCCAGG GCCCAGAAGT CAGCCTGATC AGCCAGAAAG AGGTCGTCTG GAGTACAGCA 
GGCAGCCCGG TCCCTCCCCT CCCCAAGGAA GAGGCTGCTC AAGGCCTGAC AATTCCTGCC 
GTCCCATCCC CCAAACAGGA AAAGGTTGTC CAGGGGTCTG AAGAAAGCAT CGTCTCATCT 
CCCACCCAAA AAGAAGTTGT CCAGGACCCT GATGCTTCTA ATGTCCCATC TTCCACCCAG 
AAAGTAGCTG TCCAGGCCCC AGCCACTCTC ATCGCCCCCT CCTCCTCAGT GGGCAAGGTA 
TCCTCTGGTC CAGGAGGTCC CCCAGCCCCG GTGATGATGG GAGCAGAGAC CAGCCAGGAG 
TCCCAGCTTG TCCCTGAAGC CACCAAGGAC AAGACATTCC TGGAGCCATC GAGGGAGGAG 
GAGGAGGTGG CCCTGACTGC TGACCTGGAG ATTTTCCTGG ATACCCTGCG GAGCATGGAG 
CCTCCTGAGA TCCTGCGCAC TCACCGGCTA CCTCGAGCCC CCCGCTCCTC CTACCTGGCC 
ATGTACGCCA CCCTGCCTGC CATCGAGGAG GACCAGCCTG GGCCATGTGT GCTGGGACCC 
AGCCCCCAGG AGGTGCCAGC CCTGGAAGTA AAGAAGGAGG AGAAGGCACA GGAGGAGGAG 
GAAGAAGAGC CAGAGAACCC CTACCTGAGC GACGATGAAA AGCTCCAGCG CAGGCAGGAG 
AGAGCCAGGC TAGGCCCCCC CAGGGACCCC TCCCCTGCCC GGCCCACCCA GGTCTCTTGC 
TCTCCTCTGG AGATGATGAA GAAGCATGTA GCAGGTGCCA AGGGCCCCCA CCCAGAACCT 
GGGCCAGAGT GGCAGGCAGG CAGCCGGCCC ACTTCCCGTC TTGGAGGCAG CCTCCTCTTT 
GGGGGTCTGG TGCCTGCCCT GAAGGAGGCC CCCACCTCGG AACCACTGGG CACAAAACTA 
TCCGCTCTGC AGCCCCACAG GGCACCGGGG CTCAAGAAGG GTCCAGGACA GCTGCCCCTG 
CTCTACAGTG AAAGGCCACC TCCAGAAAAG CCGGCACGTG CCCGGCCCCC GGAGGGGTGG 
AGCCCAGCAC TGAAGACCCA GGGCAAGCTG AACACGAGAC CTGGAAAGAT TATCCTCTTC 
TCAGAGCCTG GCTGCCAAGG CAGCCGCAGG GAGGTCTGGG AAGATGCTGA TGATGCTTCC 
GGCTGGGCCC GTGTGGCCTC CGTACGGGTG GTCCGAGGCT GCTGGGTGCT GCACGAACAG 
CCAGGATTCC AGGGCCAGAA GCTGGTCCTG CCTCAAGGGG ATATGGAACT TGGAGCCCCA 
GGATCAGCAT GGAGTAGCCA AGGCATCGGC TCCCTGAGGA GGGTCGTCCG GGATTACATT 
ACCCCAGAGA TCAGTCTGTA CTCTGAGGAG GGCCTCAAAG GGGAGCAAGT GAAGCTGACT 
GAGGCCTTGG AGGACCCCCA GGGCCTGGAG AGGCCCCTGC AAATGGCATC TGCCACTGTT 
TCTGCAGGAC TGTGGCTTCT ATACCCCAAA CCCTTCTTTG AAGATACTCC CTGTATTCTG 
GAGCCTGGAG AGTATCCCAC CTCAGAGGCC TGGGGAGCAT CAGACCCCAG CATGGGCTCC 
CTGAAGCCCA TGAGATTGGG CTGCCCAAGT GTGGAGAGGC CAGGGGAGCC CAAGGCTGTG 
GTGTATGAGG CCCCAGGGTT TCAGGGCCGG AGCTGGGAAG TGAACAGAGA CATCTACAAC 
CTGCAGCAGC CAGAGGATAG CCAGAGCCCC AACTTGGCGT CTGTGGGGTC CCTGCGAGTT 
CTCGGGGGCT GCTGGGTGGG CTACGAGAAG GAGGGTTTCC GGGGTCACCA GTATCTGCTG 
GAAGAGGGAG AATACACTGA TTGGTCACGT TGGGGAGGCT ATAACGAGGT GTTAACCTCC 
CTGCGGGTCA TCCGAACGGA CTTCGGGGAC CCGGCCGTGG TGCTGTTTGA GGCCATGGAC 
TTCGAGGGGC CGGGCGTGGA GGTGAGCGAG GCACTGCCAG ACGTGGAGCT GGCGGGACAC 
GGCCCCAGAA CGCAGGCGAT CCACGTTCTC AGCGGCGTGT GGGTGGCGTA TCAGGAGGTG 
GGCTTCTCCG GGGAGCAGTA CGTGCTGGAG AAGGGCGTGT ACCGCAACTG CGACGACTGG 
GGCTCGGGCA ACAGCGCCCT CGCCTCGCTG CAGCCCGTCC TGCAGGTCGG GGAGCACAAT 
CTGCACTTTA TCTCAAAGAT TCAGCTTTTC TCCGGCCCCG ACTTCCTGGG TGACCACATC 
TCTTTTGAAG ATGACCAGAG CTCTCTGCCC ACCTCCTTCC AGCCCCAGTC TTGCCGAGTC 
CACGGCGGCA GCTGGATCCT GTTTGATGAG AAGAATTTTG AGGGTGAGCA GTACATTCTG 
TCTGAGGGCG AGTTCCCTAC TCTTACAGCC ATGGGCTGCC TGGCCTCCAC CGTCCTGGGC 
TCTCTCCGGA AGGTGCCCCT GTACTTTTCC GAGCCATCCA TTTTCCTGTA TGGACTGGAG 
TGCTTCGAGG GCAAGGAGAT TGAGCTCAGC GGGGAGGTGC GGAGCCTGCA GGCCGAGGGC 
TTCAACAATC ATGTGCTGTC CGTGCGGATC AAGGGGGGCG TCTGGGTGCT ATGCGAGCAC 
AGCGACTTCC GGGGCCGCCA GTGGCTGGTG GGCAGCTGTG AGATCACCAA CTGGCTGACA 
TACAGCGGGA CCCAGCGGGT GGGCTCCCTC TACCCCATCA AGCAGCGCCG GGCTTATTTT 
CGCCTCTGGA ATGCGGCACT GGGGGGATTC CTGACTGTGC CAGACCACGT GGAAGACATG 
AAGGCGGGCC GCGTGGTGGT CTCTGAGCCC CAAGCCGGAG GCAGCAACAT CTGGTTCTAC 
GAGGATGGGC TACTGAAGAA CCAGGTGGCC CCCACTATGA GCCTGCAGGT GATTGGCCCC 
CCCAGCACAG GCTCCAAGGT GGTGCTGTGG GCTGAGAGCC GCCTGCCCCG CCAGACATGG 
AGCATCAGTG AGTTGGGCTA CATCTGCAGC CACATGTTTG AAGGTCGGAT CCTAGATGTG 
AAAGGTGGCC GGGGCTACGA CCGGGACCAC GCAGTGCTGT GGGAGCTGGC CAAGGACAGG 
GCGTCCCAGA TCTGGACTGT CCATGTGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315568.1
CDS	973..6645
Translation	MPKELSRALPLCPALVALAASPEGNSAWCQSSAQLLRCQGGQPGLSWGTQGAGQRGCHWVSASAFPQAQAQSHSELDLRAGSLGSWLWKRRHSSVGPVEPLGRLQRPAVGSASDLANHASMGPESLAPAVTSGEPAGARGPSGPPPVPGCLEQGPPGHTCQQPPHSARGFPLWPKAPSNLEDVLPHPASCPRAEGAKGEPTTSGPALPEPTLPEPAQTLRERPSARRTRYYRITVSLQGREQAPGEETEEPKPAQPAPHPCGPEESRGWQEPPQGPRPITGCLADPPQEPQLRAPPHQGSTVQQQELRAGRTPGSPHRRELDHSEPRTPQDALPGPNMEEASGPPAEAKARVVSTTLTWRQRPPAQDEVKHRFHKVSLVSGAQSEAPSEETSESSHRREEVNGFAVREEETVNHQGPRDGAGSKSFQSHGPIFSKKYTLPLKEKRPVGRLKEAAEQGDGSPQPPRTEPPSAGVTARTELSVPAHGPREPSPHPGVSFTSGSSRSLEERRVTRTVRTTVMVGGHVDRRVNSTVINSVGPALVGEALPRGRNAARAVRAVVVNPRAEGSPSRSQALELLSSLVPTERTAPTSRLPRPLAAVPRSPDLGSTEGSALRQLPETRTAEPKDRSAPGPAGFLEEAHPTPSQEVPAAHPDQEQDRTLGAGVHEVPRVQRPASPTQSPLQTSQGHTPVPSSPRLQTRTLSLDLVQPPEQPEAPTQARAQLTLKPQGPQALPSIKREGLSDPPAATVLPMVKTEVVIVPGQSLAPSSMRWKAITSPGGLSAPSSPKNKVVQDSEKVPVVSLTQKEIVQGPGVPADSSPNQKEVVQGRSAPAASGPKPTNVAQGPEVSLISQKEVVWSTAGSPVPPLPKEEAAQGLTIPAVPSPKQEKVVQGSEESIVSSPTQKEVVQDPDASNVPSSTQKVAVQAPATLIAPSSSVGKVSSGPGGPPAPVMMGAETSQESQLVPEATKDKTFLEPSREEEEVALTADLEIFLDTLRSMEPPEILRTHRLPRAPRSSYLAMYATLPAIEEDQPGPCVLGPSPQEVPALEVKKEEKAQEEEEEEPENPYLSDDEKLQRRQERARLGPPRDPSPARPTQVSCSPLEMMKKHVAGAKGPHPEPGPEWQAGSRPTSRLGGSLLFGGLVPALKEAPTSEPLGTKLSALQPHRAPGLKKGPGQLPLLYSERPPPEKPARARPPEGWSPALKTQGKLNTRPGKIILFSEPGCQGSRREVWEDADDASGWARVASVRVVRGCWVLHEQPGFQGQKLVLPQGDMELGAPGSAWSSQGIGSLRRVVRDYITPEISLYSEEGLKGEQVKLTEALEDPQGLERPLQMASATVSAGLWLLYPKPFFEDTPCILEPGEYPTSEAWGASDPSMGSLKPMRLGCPSVERPGEPKAVVYEAPGFQGRSWEVNRDIYNLQQPEDSQSPNLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYTDWSRWGGYNEVLTSLRVIRTDFGDPAVVLFEAMDFEGPGVEVSEALPDVELAGHGPRTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCDDWGSGNSALASLQPVLQVGEHNLHFISKIQLFSGPDFLGDHISFEDDQSSLPTSFQPQSCRVHGGSWILFDEKNFEGEQYILSEGEFPTLTAMGCLASTVLGSLRKVPLYFSEPSIFLYGLECFEGKEIELSGEVRSLQAEGFNNHVLSVRIKGGVWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRAYFRLWNAALGGFLTVPDHVEDMKAGRVVVSEPQAGGSNIWFYEDGLLKNQVAPTMSLQVIGPPSTGSKVVLWAESRLPRQTWSISELGYICSHMFEGRILDVKGGRGYDRDHAVLWELAKDRASQIWTVHVL

Target ORF information:

RefSeq Version	XM_015460082.1
Organism	Bos taurus(cattle)
Definition	Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460082.1

ORF Insert Sequence:

ATGCCGAAGG AACTTTCCAG AGCTCTTCCT CTTTGTCCAG CTCTTGTGGC CCTGGCAGCC 
TCTCCGGAGG GCAACTCCGC TTGGTGTCAG AGCTCTGCTC AGCTTCTCCG GTGCCAAGGA 
GGGCAGCCAG GGCTGTCGTG GGGTACCCAG GGGGCAGGCC AGAGAGGCTG TCACTGGGTC 
TCTGCTTCTG CTTTCCCTCA GGCCCAAGCC CAGAGCCACT CAGAGTTGGA CCTTCGTGCA 
GGGAGCCTTG GCTCCTGGCT TTGGAAGCGA CGACACAGCT CAGTGGGGCC CGTGGAGCCT 
CTGGGGCGCC TCCAGAGGCC TGCTGTAGGC AGTGCCTCTG ACCTGGCCAA CCATGCCTCC 
ATGGGGCCGG AGAGCCTGGC ACCAGCGGTG ACCTCGGGAG AGCCTGCTGG AGCCAGGGGT 
CCCTCAGGCC CACCGCCGGT GCCTGGATGT TTGGAACAAG GACCTCCGGG CCACACCTGC 
CAGCAACCCC CACACTCAGC CCGGGGGTTT CCTCTGTGGC CCAAGGCACC CAGCAACCTG 
GAAGACGTGC TGCCCCACCC AGCGTCCTGC CCCAGGGCAG AGGGGGCAAA GGGCGAGCCT 
ACCACCTCAG GGCCGGCCCT GCCAGAGCCA ACCCTGCCAG AGCCAGCCCA GACCCTACGT 
GAGCGTCCCT CTGCCAGGAG GACCCGCTAC TACCGCATCA CTGTCAGCCT GCAGGGGCGT 
GAGCAAGCAC CTGGGGAGGA GACTGAAGAG CCCAAACCGG CCCAACCAGC TCCCCACCCC 
TGCGGCCCTG AGGAATCCAG GGGCTGGCAG GAGCCTCCTC AGGGGCCCCG CCCCATCACA 
GGGTGCCTAG CTGACCCGCC TCAGGAACCC CAGCTCCGAG CCCCACCACA TCAGGGGAGC 
ACGGTCCAGC AGCAGGAGCT CCGGGCCGGC CGGACGCCTG GGTCCCCGCA CAGACGCGAG 
CTGGACCACA GCGAGCCAAG GACACCTCAG GACGCCTTGC CAGGGCCCAA CATGGAGGAG 
GCCAGCGGGC CTCCGGCTGA GGCCAAGGCC CGAGTGGTGA GCACCACGCT GACATGGCGG 
CAGCGGCCCC CTGCCCAGGA CGAGGTCAAA CACCGTTTCC ACAAGGTGTC CCTGGTGTCA 
GGGGCCCAGT CGGAAGCCCC GTCCGAGGAG ACGTCTGAGT CCAGCCATCG TCGAGAGGAA 
GTCAATGGCT TTGCTGTCCG GGAGGAAGAG ACCGTGAACC ACCAGGGCCC CCGGGACGGG 
GCTGGCTCCA AGAGCTTCCA GAGCCACGGG CCCATCTTTT CCAAGAAGTA CACATTACCC 
CTCAAGGAGA AAAGGCCAGT GGGAAGACTG AAGGAGGCTG CGGAGCAGGG TGACGGCAGC 
CCCCAGCCCC CCAGGACCGA GCCGCCCAGC GCGGGAGTCA CGGCCAGGAC TGAGTTGTCG 
GTGCCAGCAC ACGGGCCCCG GGAGCCGAGC CCCCACCCGG GTGTGAGTTT CACCAGCGGG 
AGCTCCAGGA GCCTGGAGGA GCGGCGGGTG ACACGCACTG TGCGGACCAC CGTGATGGTG 
GGAGGGCACG TGGACCGCCG GGTGAACAGC ACCGTGATCA ACTCCGTGGG GCCCGCGCTC 
GTGGGCGAGG CCCTGCCAAG GGGCCGCAAT GCTGCCCGCG CGGTGCGGGC AGTGGTGGTG 
AACCCGCGGG CCGAGGGCTC CCCCAGTCGC AGTCAGGCCC TGGAGCTGCT AAGTAGCCTT 
GTGCCCACTG AGCGGACCGC CCCCACCAGC CGGCTTCCCA GGCCCCTGGC TGCTGTGCCA 
AGGAGTCCAG ATCTGGGCAG CACAGAAGGG TCAGCCCTGA GGCAGCTGCC TGAGACGAGG 
ACAGCAGAGC CGAAGGACAG ATCTGCCCCG GGCCCTGCGG GCTTCCTGGA GGAAGCTCAC 
CCCACTCCGA GCCAGGAGGT CCCTGCAGCC CACCCAGACC AGGAACAGGA CAGAACCCTG 
GGTGCCGGGG TCCATGAGGT CCCAAGGGTG CAGAGACCCG CCAGCCCCAC CCAGTCCCCT 
CTCCAGACCT CTCAGGGCCA CACCCCAGTA CCTTCCTCTC CCAGACTCCA GACCCGAACC 
CTCTCCCTGG ACCTAGTTCA ACCCCCAGAG CAACCTGAAG CACCTACTCA GGCTCGGGCC 
CAGCTCACTC TCAAGCCCCA GGGACCCCAG GCCCTGCCCT CCATCAAAAG AGAAGGGCTT 
TCAGATCCCC CTGCGGCCAC CGTCCTTCCT ATGGTGAAGA CTGAGGTTGT GATAGTCCCT 
GGACAATCTC TTGCTCCATC CTCTATGAGA TGGAAGGCCA TCACCAGCCC GGGAGGTCTT 
TCTGCTCCAT CCTCCCCAAA GAATAAGGTT GTTCAGGACT CTGAAAAAGT CCCTGTTGTC 
TCCCTTACCC AGAAAGAGAT AGTACAGGGC CCTGGAGTTC CTGCTGACTC ATCTCCCAAC 
CAAAAAGAGG TAGTGCAGGG TCGGAGTGCT CCTGCTGCCT CAGGCCCCAA ACCAACCAAC 
GTTGCCCAGG GCCCAGAAGT CAGCCTGATC AGCCAGAAAG AGGTCGTCTG GAGTACAGCA 
GGCAGCCCGG TCCCTCCCCT CCCCAAGGAA GAGGCTGCTC AAGGCCTGAC AATTCCTGCC 
GTCCCATCCC CCAAACAGGA AAAGGTTGTC CAGGGGTCTG AAGAAAGCAT CGTCTCATCT 
CCCACCCAAA AAGAAGTTGT CCAGGACCCT GATGCTTCTA ATGTCCCATC TTCCACCCAG 
AAAGTAGCTG TCCAGGCCCC AGCCACTCTC ATCGCCCCCT CCTCCTCAGT GGGCAAGGTA 
TCCTCTGGTC CAGGAGGTCC CCCAGCCCCG GTGATGATGG GAGCAGAGAC CAGCCAGGAG 
TCCCAGCTTG TCCCTGAAGC CACCAAGGAC AAGACATTCC TGGAGCCATC GAGGGAGGAG 
GAGGAGGTGG CCCTGACTGC TGACCTGGAG ATTTTCCTGG ATACCCTGCG GAGCATGGAG 
CCTCCTGAGA TCCTGCGCAC TCACCGGCTA CCTCGAGCCC CCCGCTCCTC CTACCTGGCC 
ATGTACGCCA CCCTGCCTGC CATCGAGGAG GACCAGCCTG GGCCATGTGT GCTGGGACCC 
AGCCCCCAGG AGGTGCCAGC CCTGGAAGTA AAGAAGGAGG AGAAGGCACA GGAGGAGGAG 
GAAGAAGAGC CAGAGAACCC CTACCTGAGC GACGATGAAA AGCTCCAGCG CAGGCAGGAG 
AGAGCCAGGC TAGGCCCCCC CAGGGACCCC TCCCCTGCCC GGCCCACCCA GGTCTCTTGC 
TCTCCTCTGG AGATGATGAA GAAGCATGTA GCAGGTGCCA AGGGCCCCCA CCCAGAACCT 
GGGCCAGAGT GGCAGGCAGG CAGCCGGCCC ACTTCCCGTC TTGGAGGCAG CCTCCTCTTT 
GGGGGTCTGG TGCCTGCCCT GAAGGAGGCC CCCACCTCGG AACCACTGGG CACAAAACTA 
TCCGCTCTGC AGCCCCACAG GGCACCGGGG CTCAAGAAGG GTCCAGGACA GCTGCCCCTG 
CTCTACAGTG AAAGGCCACC TCCAGAAAAG CCGGCACGTG CCCGGCCCCC GGAGGGGTGG 
AGCCCAGCAC TGAAGACCCA GGGCAAGCTG AACACGAGAC CTGGAAAGAT TATCCTCTTC 
TCAGAGCCTG GCTGCCAAGG CAGCCGCAGG GAGGTCTGGG AAGATGCTGA TGATGCTTCC 
GGCTGGGCCC GTGTGGCCTC CGTACGGGTG GTCCGAGGCT GCTGGGTGCT GCACGAACAG 
CCAGGATTCC AGGGCCAGAA GCTGGTCCTG CCTCAAGGGG ATATGGAACT TGGAGCCCCA 
GGATCAGCAT GGAGTAGCCA AGGCATCGGC TCCCTGAGGA GGGTCGTCCG GGATTACATT 
ACCCCAGAGA TCAGTCTGTA CTCTGAGGAG GGCCTCAAAG GGGAGCAAGT GAAGCTGACT 
GAGGCCTTGG AGGACCCCCA GGGCCTGGAG AGGCCCCTGC AAATGGCATC TGCCACTGTT 
TCTGCAGGAC TGTGGCTTCT ATACCCCAAA CCCTTCTTTG AAGATACTCC CTGTATTCTG 
GAGCCTGGAG AGTATCCCAC CTCAGAGGCC TGGGGAGCAT CAGACCCCAG CATGGGCTCC 
CTGAAGCCCA TGAGATTGGG CTGCCCAAGT GTGGAGAGGC CAGGGGAGCC CAAGGCTGTG 
GTGTATGAGG CCCCAGGGTT TCAGGGCCGG AGCTGGGAAG TGAACAGAGA CATCTACAAC 
CTGCAGCAGC CAGAGGATAG CCAGAGCCCC AACTTGGCGT CTGTGGGGTC CCTGCGAGTT 
CTCGGGGGCT GCTGGGTGGG CTACGAGAAG GAGGGTTTCC GGGGTCACCA GTATCTGCTG 
GAAGAGGGAG AATACACTGA TTGGTCACGT TGGGGAGGCT ATAACGAGGT GTTAACCTCC 
CTGCGGGTCA TCCGAACGGA CTTCGGGGAC CCGGCCGTGG TGCTGTTTGA GGCCATGGAC 
TTCGAGGGGC CGGGCGTGGA GGTGAGCGAG GCACTGCCAG ACGTGGAGCT GGCGGGACAC 
GGCCCCAGAA CGCAGGCGAT CCACGTTCTC AGCGGCGTGT GGGTGGCGTA TCAGGAGGTG 
GGCTTCTCCG GGGAGCAGTA CGTGCTGGAG AAGGGCGTGT ACCGCAACTG CGACGACTGG 
GGCTCGGGCA ACAGCGCCCT CGCCTCGCTG CAGCCCGTCC TGCAGGTCGG GGAGCACAAT 
CTGCACTTTA TCTCAAAGAT TCAGCTTTTC TCCGGCCCCG ACTTCCTGGG TGACCACATC 
TCTTTTGAAG ATGACCAGAG CTCTCTGCCC ACCTCCTTCC AGCCCCAGTC TTGCCGAGTC 
CACGGCGGCA GCTGGATCCT GTTTGATGAG AAGAATTTTG AGGGTGAGCA GTACATTCTG 
TCTGAGGGCG AGTTCCCTAC TCTTACAGCC ATGGGCTGCC TGGCCTCCAC CGTCCTGGGC 
TCTCTCCGGA AGGTGCCCCT GTACTTTTCC GAGCCATCCA TTTTCCTGTA TGGACTGGAG 
TGCTTCGAGG GCAAGGAGAT TGAGCTCAGC GGGGAGGTGC GGAGCCTGCA GGCCGAGGGC 
TTCAACAATC ATGTGCTGTC CGTGCGGATC AAGGGGGGCG TCTGGGTGCT ATGCGAGCAC 
AGCGACTTCC GGGGCCGCCA GTGGCTGGTG GGCAGCTGTG AGATCACCAA CTGGCTGACA 
TACAGCGGGA CCCAGCGGGT GGGCTCCCTC TACCCCATCA AGCAGCGCCG GGCTTATTTT 
CGCCTCTGGA ATGCGGCACT GGGGGGATTC CTGACTGTGC CAGACCACGT GGAAGACATG 
AAGGCGGGCC GCGTGGTGGT CTCTGAGCCC CAAGCCGGAG GCAGCAACAT CTGGTTCTAC 
GAGGATGGGC TACTGAAGAA CCAGGTGGCC CCCACTATGA GCCTGCAGGT GATTGGCCCC 
CCCAGCACAG GCTCCAAGGT GGTGCTGTGG GCTGAGAGCC GCCTGCCCCG CCAGACATGG 
AGCATCAGTG AGTTGGGCTA CATCTGCAGC CACATGTTTG AAGGTCGGAT CCTAGATGTG 
AAAGGTGGCC GGGGCTACGA CCGGGACCAC GCAGTGCTGT GGGAGCTGGC CAAGGACAGG 
GCGTCCCAGA TCTGGACTGT CCATGTGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG2 ( XM_015460083.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315569.1 Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X3, mRNA.

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CloneID	OBa88051
Clone ID Related Accession (Same CDS sequence)	XM_015460083.1 , XM_015460083.2
Accession Version	XM_015460083.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	absent in melanoma 1-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGG AGAGCCTGGC ACCAGCGGTG ACCTCGGGAG AGCCTGCTGG AGCCAGGGGT 
CCCTCAGGCC CACCGCCGGT GCCTGGATGT TTGGAACAAG GACCTCCGGG CCACACCTGC 
CAGCAACCCC CACACTCAGC CCGGGGGTTT CCTCTGTGGC CCAAGGCACC CAGCAACCTG 
GAAGACGTGC TGCCCCACCC AGCGTCCTGC CCCAGGGCAG AGGGGGCAAA GGGCGAGCCT 
ACCACCTCAG GGCCGGCCCT GCCAGAGCCA ACCCTGCCAG AGCCAGCCCA GACCCTACGT 
GAGCGTCCCT CTGCCAGGAG GACCCGCTAC TACCGCATCA CTGTCAGCCT GCAGGGGCGT 
GAGCAAGCAC CTGGGGAGGA GACTGAAGAG CCCAAACCGG CCCAACCAGC TCCCCACCCC 
TGCGGCCCTG AGGAATCCAG GGGCTGGCAG GAGCCTCCTC AGGGGCCCCG CCCCATCACA 
GGGTGCCTAG CTGACCCGCC TCAGGAACCC CAGCTCCGAG CCCCACCACA TCAGGGGAGC 
ACGGTCCAGC AGCAGGAGCT CCGGGCCGGC CGGACGCCTG GGTCCCCGCA CAGACGCGAG 
CTGGACCACA GCGAGCCAAG GACACCTCAG GACGCCTTGC CAGGGCCCAA CATGGAGGAG 
GCCAGCGGGC CTCCGGCTGA GGCCAAGGCC CGAGTGGTGA GCACCACGCT GACATGGCGG 
CAGCGGCCCC CTGCCCAGGA CGAGGTCAAA CACCGTTTCC ACAAGGTGTC CCTGGTGTCA 
GGGGCCCAGT CGGAAGCCCC GTCCGAGGAG ACGTCTGAGT CCAGCCATCG TCGAGAGGAA 
GTCAATGGCT TTGCTGTCCG GGAGGAAGAG ACCGTGAACC ACCAGGGCCC CCGGGACGGG 
GCTGGCTCCA AGAGCTTCCA GAGCCACGGG CCCATCTTTT CCAAGAAGTA CACATTACCC 
CTCAAGGAGA AAAGGCCAGT GGGAAGACTG AAGGAGGCTG CGGAGCAGGG TGACGGCAGC 
CCCCAGCCCC CCAGGACCGA GCCGCCCAGC GCGGGAGTCA CGGCCAGGAC TGAGTTGTCG 
GTGCCAGCAC ACGGGCCCCG GGAGCCGAGC CCCCACCCGG GTGTGAGTTT CACCAGCGGG 
AGCTCCAGGA GCCTGGAGGA GCGGCGGGTG ACACGCACTG TGCGGACCAC CGTGATGGTG 
GGAGGGCACG TGGACCGCCG GGTGAACAGC ACCGTGATCA ACTCCGTGGG GCCCGCGCTC 
GTGGGCGAGG CCCTGCCAAG GGGCCGCAAT GCTGCCCGCG CGGTGCGGGC AGTGGTGGTG 
AACCCGCGGG CCGAGGGCTC CCCCAGTCGC AGTCAGGCCC TGGAGCTGCT AAGTAGCCTT 
GTGCCCACTG AGCGGACCGC CCCCACCAGC CGGCTTCCCA GGCCCCTGGC TGCTGTGCCA 
AGGAGTCCAG ATCTGGGCAG CACAGAAGGG TCAGCCCTGA GGCAGCTGCC TGAGACGAGG 
ACAGCAGAGC CGAAGGACAG ATCTGCCCCG GGCCCTGCGG GCTTCCTGGA GGAAGCTCAC 
CCCACTCCGA GCCAGGAGGT CCCTGCAGCC CACCCAGACC AGGAACAGGA CAGAACCCTG 
GGTGCCGGGG TCCATGAGGT CCCAAGGGTG CAGAGACCCG CCAGCCCCAC CCAGTCCCCT 
CTCCAGACCT CTCAGGGCCA CACCCCAGTA CCTTCCTCTC CCAGACTCCA GACCCGAACC 
CTCTCCCTGG ACCTAGTTCA ACCCCCAGAG CAACCTGAAG CACCTACTCA GGCTCGGGCC 
CAGCTCACTC TCAAGCCCCA GGGACCCCAG GCCCTGCCCT CCATCAAAAG AGAAGGGCTT 
TCAGATCCCC CTGCGGCCAC CGTCCTTCCT ATGGTGAAGA CTGAGGTTGT GATAGTCCCT 
GGACAATCTC TTGCTCCATC CTCTATGAGA TGGAAGGCCA TCACCAGCCC GGGAGGTCTT 
TCTGCTCCAT CCTCCCCAAA GAATAAGGTT GTTCAGGACT CTGAAAAAGT CCCTGTTGTC 
TCCCTTACCC AGAAAGAGAT AGTACAGGGC CCTGGAGTTC CTGCTGACTC ATCTCCCAAC 
CAAAAAGAGG TAGTGCAGGG TCGGAGTGCT CCTGCTGCCT CAGGCCCCAA ACCAACCAAC 
GTTGCCCAGG GCCCAGAAGT CAGCCTGATC AGCCAGAAAG AGGTCGTCTG GAGTACAGCA 
GGCAGCCCGG TCCCTCCCCT CCCCAAGGAA GAGGCTGCTC AAGGCCTGAC AATTCCTGCC 
GTCCCATCCC CCAAACAGGA AAAGGTTGTC CAGGGGTCTG AAGAAAGCAT CGTCTCATCT 
CCCACCCAAA AAGAAGTTGT CCAGGACCCT GATGCTTCTA ATGTCCCATC TTCCACCCAG 
AAAGTAGCTG TCCAGGCCCC AGCCACTCTC ATCGCCCCCT CCTCCTCAGT GGGCAAGGTA 
TCCTCTGGTC CAGGAGGTCC CCCAGCCCCG GTGATGATGG GAGCAGAGAC CAGCCAGGAG 
TCCCAGCTTG TCCCTGAAGC CACCAAGGAC AAGACATTCC TGGAGCCATC GAGGGAGGAG 
GAGGAGGTGG CCCTGACTGC TGACCTGGAG ATTTTCCTGG ATACCCTGCG GAGCATGGAG 
CCTCCTGAGA TCCTGCGCAC TCACCGGCTA CCTCGAGCCC CCCGCTCCTC CTACCTGGCC 
ATGTACGCCA CCCTGCCTGC CATCGAGGAG GACCAGCCTG GGCCATGTGT GCTGGGACCC 
AGCCCCCAGG AGGTGCCAGC CCTGGAAGTA AAGAAGGAGG AGAAGGCACA GGAGGAGGAG 
GAAGAAGAGC CAGAGAACCC CTACCTGAGC GACGATGAAA AGCTCCAGCG CAGGCAGGAG 
AGAGCCAGGC TAGGCCCCCC CAGGGACCCC TCCCCTGCCC GGCCCACCCA GGTCTCTTGC 
TCTCCTCTGG AGATGATGAA GAAGCATGTA GCAGGTGCCA AGGGCCCCCA CCCAGAACCT 
GGGCCAGAGT GGCAGGCAGG CAGCCGGCCC ACTTCCCGTC TTGGAGGCAG CCTCCTCTTT 
GGGGGTCTGG TGCCTGCCCT GAAGGAGGCC CCCACCTCGG AACCACTGGG CACAAAACTA 
TCCGCTCTGC AGCCCCACAG GGCACCGGGG CTCAAGAAGG GTCCAGGACA GCTGCCCCTG 
CTCTACAGTG AAAGGCCACC TCCAGAAAAG CCGGCACGTG CCCGGCCCCC GGAGGGGTGG 
AGCCCAGCAC TGAAGACCCA GGGCAAGCTG AACACGAGAC CTGGAAAGAT TATCCTCTTC 
TCAGAGCCTG GCTGCCAAGG CAGCCGCAGG GAGGTCTGGG AAGATGCTGA TGATGCTTCC 
GGCTGGGCCC GTGTGGCCTC CGTACGGGTG GTCCGAGGCT GCTGGGTGCT GCACGAACAG 
CCAGGATTCC AGGGCCAGAA GCTGGTCCTG CCTCAAGGGG ATATGGAACT TGGAGCCCCA 
GGATCAGCAT GGAGTAGCCA AGGCATCGGC TCCCTGAGGA GGGTCGTCCG GGATTACATT 
ACCCCAGAGA TCAGTCTGTA CTCTGAGGAG GGCCTCAAAG GGGAGCAAGT GAAGCTGACT 
GAGGCCTTGG AGGACCCCCA GGGCCTGGAG AGGCCCCTGC AAATGGCATC TGCCACTGTT 
TCTGCAGGAC TGTGGCTTCT ATACCCCAAA CCCTTCTTTG AAGATACTCC CTGTATTCTG 
GAGCCTGGAG AGTATCCCAC CTCAGAGGCC TGGGGAGCAT CAGACCCCAG CATGGGCTCC 
CTGAAGCCCA TGAGATTGGG CTGCCCAAGT GTGGAGAGGC CAGGGGAGCC CAAGGCTGTG 
GTGTATGAGG CCCCAGGGTT TCAGGGCCGG AGCTGGGAAG TGAACAGAGA CATCTACAAC 
CTGCAGCAGC CAGAGGATAG CCAGAGCCCC AACTTGGCGT CTGTGGGGTC CCTGCGAGTT 
CTCGGGGGCT GCTGGGTGGG CTACGAGAAG GAGGGTTTCC GGGGTCACCA GTATCTGCTG 
GAAGAGGGAG AATACACTGA TTGGTCACGT TGGGGAGGCT ATAACGAGGT GTTAACCTCC 
CTGCGGGTCA TCCGAACGGA CTTCGGGGAC CCGGCCGTGG TGCTGTTTGA GGCCATGGAC 
TTCGAGGGGC CGGGCGTGGA GGTGAGCGAG GCACTGCCAG ACGTGGAGCT GGCGGGACAC 
GGCCCCAGAA CGCAGGCGAT CCACGTTCTC AGCGGCGTGT GGGTGGCGTA TCAGGAGGTG 
GGCTTCTCCG GGGAGCAGTA CGTGCTGGAG AAGGGCGTGT ACCGCAACTG CGACGACTGG 
GGCTCGGGCA ACAGCGCCCT CGCCTCGCTG CAGCCCGTCC TGCAGGTCGG GGAGCACAAT 
CTGCACTTTA TCTCAAAGAT TCAGCTTTTC TCCGGCCCCG ACTTCCTGGG TGACCACATC 
TCTTTTGAAG ATGACCAGAG CTCTCTGCCC ACCTCCTTCC AGCCCCAGTC TTGCCGAGTC 
CACGGCGGCA GCTGGATCCT GTTTGATGAG AAGAATTTTG AGGGTGAGCA GTACATTCTG 
TCTGAGGGCG AGTTCCCTAC TCTTACAGCC ATGGGCTGCC TGGCCTCCAC CGTCCTGGGC 
TCTCTCCGGA AGGTGCCCCT GTACTTTTCC GAGCCATCCA TTTTCCTGTA TGGACTGGAG 
TGCTTCGAGG GCAAGGAGAT TGAGCTCAGC GGGGAGGTGC GGAGCCTGCA GGCCGAGGGC 
TTCAACAATC ATGTGCTGTC CGTGCGGATC AAGGGGGGCG TCTGGGTGCT ATGCGAGCAC 
AGCGACTTCC GGGGCCGCCA GTGGCTGGTG GGCAGCTGTG AGATCACCAA CTGGCTGACA 
TACAGCGGGA CCCAGCGGGT GGGCTCCCTC TACCCCATCA AGCAGCGCCG GGCTTATTTT 
CGCCTCTGGA ATGCGGCACT GGGGGGATTC CTGACTGTGC CAGACCACGT GGAAGACATG 
AAGGCGGGCC GCGTGGTGGT CTCTGAGCCC CAAGCCGGAG GCAGCAACAT CTGGTTCTAC 
GAGGATGGGC TACTGAAGAA CCAGGTGGCC CCCACTATGA GCCTGCAGGT GATTGGCCCC 
CCCAGCACAG GCTCCAAGGT GGTGCTGTGG GCTGAGAGCC GCCTGCCCCG CCAGACATGG 
AGCATCAGTG AGTTGGGCTA CATCTGCAGC CACATGTTTG AAGGTCGGAT CCTAGATGTG 
AAAGGTGGCC GGGGCTACGA CCGGGACCAC GCAGTGCTGT GGGAGCTGGC CAAGGACAGG 
GCGTCCCAGA TCTGGACTGT CCATGTGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315569.1
CDS	248..5560
Translation	MGPESLAPAVTSGEPAGARGPSGPPPVPGCLEQGPPGHTCQQPPHSARGFPLWPKAPSNLEDVLPHPASCPRAEGAKGEPTTSGPALPEPTLPEPAQTLRERPSARRTRYYRITVSLQGREQAPGEETEEPKPAQPAPHPCGPEESRGWQEPPQGPRPITGCLADPPQEPQLRAPPHQGSTVQQQELRAGRTPGSPHRRELDHSEPRTPQDALPGPNMEEASGPPAEAKARVVSTTLTWRQRPPAQDEVKHRFHKVSLVSGAQSEAPSEETSESSHRREEVNGFAVREEETVNHQGPRDGAGSKSFQSHGPIFSKKYTLPLKEKRPVGRLKEAAEQGDGSPQPPRTEPPSAGVTARTELSVPAHGPREPSPHPGVSFTSGSSRSLEERRVTRTVRTTVMVGGHVDRRVNSTVINSVGPALVGEALPRGRNAARAVRAVVVNPRAEGSPSRSQALELLSSLVPTERTAPTSRLPRPLAAVPRSPDLGSTEGSALRQLPETRTAEPKDRSAPGPAGFLEEAHPTPSQEVPAAHPDQEQDRTLGAGVHEVPRVQRPASPTQSPLQTSQGHTPVPSSPRLQTRTLSLDLVQPPEQPEAPTQARAQLTLKPQGPQALPSIKREGLSDPPAATVLPMVKTEVVIVPGQSLAPSSMRWKAITSPGGLSAPSSPKNKVVQDSEKVPVVSLTQKEIVQGPGVPADSSPNQKEVVQGRSAPAASGPKPTNVAQGPEVSLISQKEVVWSTAGSPVPPLPKEEAAQGLTIPAVPSPKQEKVVQGSEESIVSSPTQKEVVQDPDASNVPSSTQKVAVQAPATLIAPSSSVGKVSSGPGGPPAPVMMGAETSQESQLVPEATKDKTFLEPSREEEEVALTADLEIFLDTLRSMEPPEILRTHRLPRAPRSSYLAMYATLPAIEEDQPGPCVLGPSPQEVPALEVKKEEKAQEEEEEEPENPYLSDDEKLQRRQERARLGPPRDPSPARPTQVSCSPLEMMKKHVAGAKGPHPEPGPEWQAGSRPTSRLGGSLLFGGLVPALKEAPTSEPLGTKLSALQPHRAPGLKKGPGQLPLLYSERPPPEKPARARPPEGWSPALKTQGKLNTRPGKIILFSEPGCQGSRREVWEDADDASGWARVASVRVVRGCWVLHEQPGFQGQKLVLPQGDMELGAPGSAWSSQGIGSLRRVVRDYITPEISLYSEEGLKGEQVKLTEALEDPQGLERPLQMASATVSAGLWLLYPKPFFEDTPCILEPGEYPTSEAWGASDPSMGSLKPMRLGCPSVERPGEPKAVVYEAPGFQGRSWEVNRDIYNLQQPEDSQSPNLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYTDWSRWGGYNEVLTSLRVIRTDFGDPAVVLFEAMDFEGPGVEVSEALPDVELAGHGPRTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCDDWGSGNSALASLQPVLQVGEHNLHFISKIQLFSGPDFLGDHISFEDDQSSLPTSFQPQSCRVHGGSWILFDEKNFEGEQYILSEGEFPTLTAMGCLASTVLGSLRKVPLYFSEPSIFLYGLECFEGKEIELSGEVRSLQAEGFNNHVLSVRIKGGVWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRAYFRLWNAALGGFLTVPDHVEDMKAGRVVVSEPQAGGSNIWFYEDGLLKNQVAPTMSLQVIGPPSTGSKVVLWAESRLPRQTWSISELGYICSHMFEGRILDVKGGRGYDRDHAVLWELAKDRASQIWTVHVL

Target ORF information:

RefSeq Version	XM_015460083.1
Organism	Bos taurus(cattle)
Definition	Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460083.1

ORF Insert Sequence:

ATGGGGCCGG AGAGCCTGGC ACCAGCGGTG ACCTCGGGAG AGCCTGCTGG AGCCAGGGGT 
CCCTCAGGCC CACCGCCGGT GCCTGGATGT TTGGAACAAG GACCTCCGGG CCACACCTGC 
CAGCAACCCC CACACTCAGC CCGGGGGTTT CCTCTGTGGC CCAAGGCACC CAGCAACCTG 
GAAGACGTGC TGCCCCACCC AGCGTCCTGC CCCAGGGCAG AGGGGGCAAA GGGCGAGCCT 
ACCACCTCAG GGCCGGCCCT GCCAGAGCCA ACCCTGCCAG AGCCAGCCCA GACCCTACGT 
GAGCGTCCCT CTGCCAGGAG GACCCGCTAC TACCGCATCA CTGTCAGCCT GCAGGGGCGT 
GAGCAAGCAC CTGGGGAGGA GACTGAAGAG CCCAAACCGG CCCAACCAGC TCCCCACCCC 
TGCGGCCCTG AGGAATCCAG GGGCTGGCAG GAGCCTCCTC AGGGGCCCCG CCCCATCACA 
GGGTGCCTAG CTGACCCGCC TCAGGAACCC CAGCTCCGAG CCCCACCACA TCAGGGGAGC 
ACGGTCCAGC AGCAGGAGCT CCGGGCCGGC CGGACGCCTG GGTCCCCGCA CAGACGCGAG 
CTGGACCACA GCGAGCCAAG GACACCTCAG GACGCCTTGC CAGGGCCCAA CATGGAGGAG 
GCCAGCGGGC CTCCGGCTGA GGCCAAGGCC CGAGTGGTGA GCACCACGCT GACATGGCGG 
CAGCGGCCCC CTGCCCAGGA CGAGGTCAAA CACCGTTTCC ACAAGGTGTC CCTGGTGTCA 
GGGGCCCAGT CGGAAGCCCC GTCCGAGGAG ACGTCTGAGT CCAGCCATCG TCGAGAGGAA 
GTCAATGGCT TTGCTGTCCG GGAGGAAGAG ACCGTGAACC ACCAGGGCCC CCGGGACGGG 
GCTGGCTCCA AGAGCTTCCA GAGCCACGGG CCCATCTTTT CCAAGAAGTA CACATTACCC 
CTCAAGGAGA AAAGGCCAGT GGGAAGACTG AAGGAGGCTG CGGAGCAGGG TGACGGCAGC 
CCCCAGCCCC CCAGGACCGA GCCGCCCAGC GCGGGAGTCA CGGCCAGGAC TGAGTTGTCG 
GTGCCAGCAC ACGGGCCCCG GGAGCCGAGC CCCCACCCGG GTGTGAGTTT CACCAGCGGG 
AGCTCCAGGA GCCTGGAGGA GCGGCGGGTG ACACGCACTG TGCGGACCAC CGTGATGGTG 
GGAGGGCACG TGGACCGCCG GGTGAACAGC ACCGTGATCA ACTCCGTGGG GCCCGCGCTC 
GTGGGCGAGG CCCTGCCAAG GGGCCGCAAT GCTGCCCGCG CGGTGCGGGC AGTGGTGGTG 
AACCCGCGGG CCGAGGGCTC CCCCAGTCGC AGTCAGGCCC TGGAGCTGCT AAGTAGCCTT 
GTGCCCACTG AGCGGACCGC CCCCACCAGC CGGCTTCCCA GGCCCCTGGC TGCTGTGCCA 
AGGAGTCCAG ATCTGGGCAG CACAGAAGGG TCAGCCCTGA GGCAGCTGCC TGAGACGAGG 
ACAGCAGAGC CGAAGGACAG ATCTGCCCCG GGCCCTGCGG GCTTCCTGGA GGAAGCTCAC 
CCCACTCCGA GCCAGGAGGT CCCTGCAGCC CACCCAGACC AGGAACAGGA CAGAACCCTG 
GGTGCCGGGG TCCATGAGGT CCCAAGGGTG CAGAGACCCG CCAGCCCCAC CCAGTCCCCT 
CTCCAGACCT CTCAGGGCCA CACCCCAGTA CCTTCCTCTC CCAGACTCCA GACCCGAACC 
CTCTCCCTGG ACCTAGTTCA ACCCCCAGAG CAACCTGAAG CACCTACTCA GGCTCGGGCC 
CAGCTCACTC TCAAGCCCCA GGGACCCCAG GCCCTGCCCT CCATCAAAAG AGAAGGGCTT 
TCAGATCCCC CTGCGGCCAC CGTCCTTCCT ATGGTGAAGA CTGAGGTTGT GATAGTCCCT 
GGACAATCTC TTGCTCCATC CTCTATGAGA TGGAAGGCCA TCACCAGCCC GGGAGGTCTT 
TCTGCTCCAT CCTCCCCAAA GAATAAGGTT GTTCAGGACT CTGAAAAAGT CCCTGTTGTC 
TCCCTTACCC AGAAAGAGAT AGTACAGGGC CCTGGAGTTC CTGCTGACTC ATCTCCCAAC 
CAAAAAGAGG TAGTGCAGGG TCGGAGTGCT CCTGCTGCCT CAGGCCCCAA ACCAACCAAC 
GTTGCCCAGG GCCCAGAAGT CAGCCTGATC AGCCAGAAAG AGGTCGTCTG GAGTACAGCA 
GGCAGCCCGG TCCCTCCCCT CCCCAAGGAA GAGGCTGCTC AAGGCCTGAC AATTCCTGCC 
GTCCCATCCC CCAAACAGGA AAAGGTTGTC CAGGGGTCTG AAGAAAGCAT CGTCTCATCT 
CCCACCCAAA AAGAAGTTGT CCAGGACCCT GATGCTTCTA ATGTCCCATC TTCCACCCAG 
AAAGTAGCTG TCCAGGCCCC AGCCACTCTC ATCGCCCCCT CCTCCTCAGT GGGCAAGGTA 
TCCTCTGGTC CAGGAGGTCC CCCAGCCCCG GTGATGATGG GAGCAGAGAC CAGCCAGGAG 
TCCCAGCTTG TCCCTGAAGC CACCAAGGAC AAGACATTCC TGGAGCCATC GAGGGAGGAG 
GAGGAGGTGG CCCTGACTGC TGACCTGGAG ATTTTCCTGG ATACCCTGCG GAGCATGGAG 
CCTCCTGAGA TCCTGCGCAC TCACCGGCTA CCTCGAGCCC CCCGCTCCTC CTACCTGGCC 
ATGTACGCCA CCCTGCCTGC CATCGAGGAG GACCAGCCTG GGCCATGTGT GCTGGGACCC 
AGCCCCCAGG AGGTGCCAGC CCTGGAAGTA AAGAAGGAGG AGAAGGCACA GGAGGAGGAG 
GAAGAAGAGC CAGAGAACCC CTACCTGAGC GACGATGAAA AGCTCCAGCG CAGGCAGGAG 
AGAGCCAGGC TAGGCCCCCC CAGGGACCCC TCCCCTGCCC GGCCCACCCA GGTCTCTTGC 
TCTCCTCTGG AGATGATGAA GAAGCATGTA GCAGGTGCCA AGGGCCCCCA CCCAGAACCT 
GGGCCAGAGT GGCAGGCAGG CAGCCGGCCC ACTTCCCGTC TTGGAGGCAG CCTCCTCTTT 
GGGGGTCTGG TGCCTGCCCT GAAGGAGGCC CCCACCTCGG AACCACTGGG CACAAAACTA 
TCCGCTCTGC AGCCCCACAG GGCACCGGGG CTCAAGAAGG GTCCAGGACA GCTGCCCCTG 
CTCTACAGTG AAAGGCCACC TCCAGAAAAG CCGGCACGTG CCCGGCCCCC GGAGGGGTGG 
AGCCCAGCAC TGAAGACCCA GGGCAAGCTG AACACGAGAC CTGGAAAGAT TATCCTCTTC 
TCAGAGCCTG GCTGCCAAGG CAGCCGCAGG GAGGTCTGGG AAGATGCTGA TGATGCTTCC 
GGCTGGGCCC GTGTGGCCTC CGTACGGGTG GTCCGAGGCT GCTGGGTGCT GCACGAACAG 
CCAGGATTCC AGGGCCAGAA GCTGGTCCTG CCTCAAGGGG ATATGGAACT TGGAGCCCCA 
GGATCAGCAT GGAGTAGCCA AGGCATCGGC TCCCTGAGGA GGGTCGTCCG GGATTACATT 
ACCCCAGAGA TCAGTCTGTA CTCTGAGGAG GGCCTCAAAG GGGAGCAAGT GAAGCTGACT 
GAGGCCTTGG AGGACCCCCA GGGCCTGGAG AGGCCCCTGC AAATGGCATC TGCCACTGTT 
TCTGCAGGAC TGTGGCTTCT ATACCCCAAA CCCTTCTTTG AAGATACTCC CTGTATTCTG 
GAGCCTGGAG AGTATCCCAC CTCAGAGGCC TGGGGAGCAT CAGACCCCAG CATGGGCTCC 
CTGAAGCCCA TGAGATTGGG CTGCCCAAGT GTGGAGAGGC CAGGGGAGCC CAAGGCTGTG 
GTGTATGAGG CCCCAGGGTT TCAGGGCCGG AGCTGGGAAG TGAACAGAGA CATCTACAAC 
CTGCAGCAGC CAGAGGATAG CCAGAGCCCC AACTTGGCGT CTGTGGGGTC CCTGCGAGTT 
CTCGGGGGCT GCTGGGTGGG CTACGAGAAG GAGGGTTTCC GGGGTCACCA GTATCTGCTG 
GAAGAGGGAG AATACACTGA TTGGTCACGT TGGGGAGGCT ATAACGAGGT GTTAACCTCC 
CTGCGGGTCA TCCGAACGGA CTTCGGGGAC CCGGCCGTGG TGCTGTTTGA GGCCATGGAC 
TTCGAGGGGC CGGGCGTGGA GGTGAGCGAG GCACTGCCAG ACGTGGAGCT GGCGGGACAC 
GGCCCCAGAA CGCAGGCGAT CCACGTTCTC AGCGGCGTGT GGGTGGCGTA TCAGGAGGTG 
GGCTTCTCCG GGGAGCAGTA CGTGCTGGAG AAGGGCGTGT ACCGCAACTG CGACGACTGG 
GGCTCGGGCA ACAGCGCCCT CGCCTCGCTG CAGCCCGTCC TGCAGGTCGG GGAGCACAAT 
CTGCACTTTA TCTCAAAGAT TCAGCTTTTC TCCGGCCCCG ACTTCCTGGG TGACCACATC 
TCTTTTGAAG ATGACCAGAG CTCTCTGCCC ACCTCCTTCC AGCCCCAGTC TTGCCGAGTC 
CACGGCGGCA GCTGGATCCT GTTTGATGAG AAGAATTTTG AGGGTGAGCA GTACATTCTG 
TCTGAGGGCG AGTTCCCTAC TCTTACAGCC ATGGGCTGCC TGGCCTCCAC CGTCCTGGGC 
TCTCTCCGGA AGGTGCCCCT GTACTTTTCC GAGCCATCCA TTTTCCTGTA TGGACTGGAG 
TGCTTCGAGG GCAAGGAGAT TGAGCTCAGC GGGGAGGTGC GGAGCCTGCA GGCCGAGGGC 
TTCAACAATC ATGTGCTGTC CGTGCGGATC AAGGGGGGCG TCTGGGTGCT ATGCGAGCAC 
AGCGACTTCC GGGGCCGCCA GTGGCTGGTG GGCAGCTGTG AGATCACCAA CTGGCTGACA 
TACAGCGGGA CCCAGCGGGT GGGCTCCCTC TACCCCATCA AGCAGCGCCG GGCTTATTTT 
CGCCTCTGGA ATGCGGCACT GGGGGGATTC CTGACTGTGC CAGACCACGT GGAAGACATG 
AAGGCGGGCC GCGTGGTGGT CTCTGAGCCC CAAGCCGGAG GCAGCAACAT CTGGTTCTAC 
GAGGATGGGC TACTGAAGAA CCAGGTGGCC CCCACTATGA GCCTGCAGGT GATTGGCCCC 
CCCAGCACAG GCTCCAAGGT GGTGCTGTGG GCTGAGAGCC GCCTGCCCCG CCAGACATGG 
AGCATCAGTG AGTTGGGCTA CATCTGCAGC CACATGTTTG AAGGTCGGAT CCTAGATGTG 
AAAGGTGGCC GGGGCTACGA CCGGGACCAC GCAGTGCTGT GGGAGCTGGC CAAGGACAGG 
GCGTCCCAGA TCTGGACTGT CCATGTGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG2 ( XM_015460083.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315569.1 Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88051
Clone ID Related Accession (Same CDS sequence)	XM_015460083.1 , XM_015460083.2
Accession Version	XM_015460083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta/gamma crystallin domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGG AGAGCCTGGC ACCAGCGGTG ACCTCGGGAG AGCCTGCTGG AGCCAGGGGT 
CCCTCAGGCC CACCGCCGGT GCCTGGATGT TTGGAACAAG GACCTCCGGG CCACACCTGC 
CAGCAACCCC CACACTCAGC CCGGGGGTTT CCTCTGTGGC CCAAGGCACC CAGCAACCTG 
GAAGACGTGC TGCCCCACCC AGCGTCCTGC CCCAGGGCAG AGGGGGCAAA GGGCGAGCCT 
ACCACCTCAG GGCCGGCCCT GCCAGAGCCA ACCCTGCCAG AGCCAGCCCA GACCCTACGT 
GAGCGTCCCT CTGCCAGGAG GACCCGCTAC TACCGCATCA CTGTCAGCCT GCAGGGGCGT 
GAGCAAGCAC CTGGGGAGGA GACTGAAGAG CCCAAACCGG CCCAACCAGC TCCCCACCCC 
TGCGGCCCTG AGGAATCCAG GGGCTGGCAG GAGCCTCCTC AGGGGCCCCG CCCCATCACA 
GGGTGCCTAG CTGACCCGCC TCAGGAACCC CAGCTCCGAG CCCCACCACA TCAGGGGAGC 
ACGGTCCAGC AGCAGGAGCT CCGGGCCGGC CGGACGCCTG GGTCCCCGCA CAGACGCGAG 
CTGGACCACA GCGAGCCAAG GACACCTCAG GACGCCTTGC CAGGGCCCAA CATGGAGGAG 
GCCAGCGGGC CTCCGGCTGA GGCCAAGGCC CGAGTGGTGA GCACCACGCT GACATGGCGG 
CAGCGGCCCC CTGCCCAGGA CGAGGTCAAA CACCGTTTCC ACAAGGTGTC CCTGGTGTCA 
GGGGCCCAGT CGGAAGCCCC GTCCGAGGAG ACGTCTGAGT CCAGCCATCG TCGAGAGGAA 
GTCAATGGCT TTGCTGTCCG GGAGGAAGAG ACCGTGAACC ACCAGGGCCC CCGGGACGGG 
GCTGGCTCCA AGAGCTTCCA GAGCCACGGG CCCATCTTTT CCAAGAAGTA CACATTACCC 
CTCAAGGAGA AAAGGCCAGT GGGAAGACTG AAGGAGGCTG CGGAGCAGGG TGACGGCAGC 
CCCCAGCCCC CCAGGACCGA GCCGCCCAGC GCGGGAGTCA CGGCCAGGAC TGAGTTGTCG 
GTGCCAGCAC ACGGGCCCCG GGAGCCGAGC CCCCACCCGG GTGTGAGTTT CACCAGCGGG 
AGCTCCAGGA GCCTGGAGGA GCGGCGGGTG ACACGCACTG TGCGGACCAC CGTGATGGTG 
GGAGGGCACG TGGACCGCCG GGTGAACAGC ACCGTGATCA ACTCCGTGGG GCCCGCGCTC 
GTGGGCGAGG CCCTGCCAAG GGGCCGCAAT GCTGCCCGCG CGGTGCGGGC AGTGGTGGTG 
AACCCGCGGG CCGAGGGCTC CCCCAGTCGC AGTCAGGCCC TGGAGCTGCT AAGTAGCCTT 
GTGCCCACTG AGCGGACCGC CCCCACCAGC CGGCTTCCCA GGCCCCTGGC TGCTGTGCCA 
AGGAGTCCAG ATCTGGGCAG CACAGAAGGG TCAGCCCTGA GGCAGCTGCC TGAGACGAGG 
ACAGCAGAGC CGAAGGACAG ATCTGCCCCG GGCCCTGCGG GCTTCCTGGA GGAAGCTCAC 
CCCACTCCGA GCCAGGAGGT CCCTGCAGCC CACCCAGACC AGGAACAGGA CAGAACCCTG 
GGTGCCGGGG TCCATGAGGT CCCAAGGGTG CAGAGACCCG CCAGCCCCAC CCAGTCCCCT 
CTCCAGACCT CTCAGGGCCA CACCCCAGTA CCTTCCTCTC CCAGACTCCA GACCCGAACC 
CTCTCCCTGG ACCTAGTTCA ACCCCCAGAG CAACCTGAAG CACCTACTCA GGCTCGGGCC 
CAGCTCACTC TCAAGCCCCA GGGACCCCAG GCCCTGCCCT CCATCAAAAG AGAAGGGCTT 
TCAGATCCCC CTGCGGCCAC CGTCCTTCCT ATGGTGAAGA CTGAGGTTGT GATAGTCCCT 
GGACAATCTC TTGCTCCATC CTCTATGAGA TGGAAGGCCA TCACCAGCCC GGGAGGTCTT 
TCTGCTCCAT CCTCCCCAAA GAATAAGGTT GTTCAGGACT CTGAAAAAGT CCCTGTTGTC 
TCCCTTACCC AGAAAGAGAT AGTACAGGGC CCTGGAGTTC CTGCTGACTC ATCTCCCAAC 
CAAAAAGAGG TAGTGCAGGG TCGGAGTGCT CCTGCTGCCT CAGGCCCCAA ACCAACCAAC 
GTTGCCCAGG GCCCAGAAGT CAGCCTGATC AGCCAGAAAG AGGTCGTCTG GAGTACAGCA 
GGCAGCCCGG TCCCTCCCCT CCCCAAGGAA GAGGCTGCTC AAGGCCTGAC AATTCCTGCC 
GTCCCATCCC CCAAACAGGA AAAGGTTGTC CAGGGGTCTG AAGAAAGCAT CGTCTCATCT 
CCCACCCAAA AAGAAGTTGT CCAGGACCCT GATGCTTCTA ATGTCCCATC TTCCACCCAG 
AAAGTAGCTG TCCAGGCCCC AGCCACTCTC ATCGCCCCCT CCTCCTCAGT GGGCAAGGTA 
TCCTCTGGTC CAGGAGGTCC CCCAGCCCCG GTGATGATGG GAGCAGAGAC CAGCCAGGAG 
TCCCAGCTTG TCCCTGAAGC CACCAAGGAC AAGACATTCC TGGAGCCATC GAGGGAGGAG 
GAGGAGGTGG CCCTGACTGC TGACCTGGAG ATTTTCCTGG ATACCCTGCG GAGCATGGAG 
CCTCCTGAGA TCCTGCGCAC TCACCGGCTA CCTCGAGCCC CCCGCTCCTC CTACCTGGCC 
ATGTACGCCA CCCTGCCTGC CATCGAGGAG GACCAGCCTG GGCCATGTGT GCTGGGACCC 
AGCCCCCAGG AGGTGCCAGC CCTGGAAGTA AAGAAGGAGG AGAAGGCACA GGAGGAGGAG 
GAAGAAGAGC CAGAGAACCC CTACCTGAGC GACGATGAAA AGCTCCAGCG CAGGCAGGAG 
AGAGCCAGGC TAGGCCCCCC CAGGGACCCC TCCCCTGCCC GGCCCACCCA GGTCTCTTGC 
TCTCCTCTGG AGATGATGAA GAAGCATGTA GCAGGTGCCA AGGGCCCCCA CCCAGAACCT 
GGGCCAGAGT GGCAGGCAGG CAGCCGGCCC ACTTCCCGTC TTGGAGGCAG CCTCCTCTTT 
GGGGGTCTGG TGCCTGCCCT GAAGGAGGCC CCCACCTCGG AACCACTGGG CACAAAACTA 
TCCGCTCTGC AGCCCCACAG GGCACCGGGG CTCAAGAAGG GTCCAGGACA GCTGCCCCTG 
CTCTACAGTG AAAGGCCACC TCCAGAAAAG CCGGCACGTG CCCGGCCCCC GGAGGGGTGG 
AGCCCAGCAC TGAAGACCCA GGGCAAGCTG AACACGAGAC CTGGAAAGAT TATCCTCTTC 
TCAGAGCCTG GCTGCCAAGG CAGCCGCAGG GAGGTCTGGG AAGATGCTGA TGATGCTTCC 
GGCTGGGCCC GTGTGGCCTC CGTACGGGTG GTCCGAGGCT GCTGGGTGCT GCACGAACAG 
CCAGGATTCC AGGGCCAGAA GCTGGTCCTG CCTCAAGGGG ATATGGAACT TGGAGCCCCA 
GGATCAGCAT GGAGTAGCCA AGGCATCGGC TCCCTGAGGA GGGTCGTCCG GGATTACATT 
ACCCCAGAGA TCAGTCTGTA CTCTGAGGAG GGCCTCAAAG GGGAGCAAGT GAAGCTGACT 
GAGGCCTTGG AGGACCCCCA GGGCCTGGAG AGGCCCCTGC AAATGGCATC TGCCACTGTT 
TCTGCAGGAC TGTGGCTTCT ATACCCCAAA CCCTTCTTTG AAGATACTCC CTGTATTCTG 
GAGCCTGGAG AGTATCCCAC CTCAGAGGCC TGGGGAGCAT CAGACCCCAG CATGGGCTCC 
CTGAAGCCCA TGAGATTGGG CTGCCCAAGT GTGGAGAGGC CAGGGGAGCC CAAGGCTGTG 
GTGTATGAGG CCCCAGGGTT TCAGGGCCGG AGCTGGGAAG TGAACAGAGA CATCTACAAC 
CTGCAGCAGC CAGAGGATAG CCAGAGCCCC AACTTGGCGT CTGTGGGGTC CCTGCGAGTT 
CTCGGGGGCT GCTGGGTGGG CTACGAGAAG GAGGGTTTCC GGGGTCACCA GTATCTGCTG 
GAAGAGGGAG AATACACTGA TTGGTCACGT TGGGGAGGCT ATAACGAGGT GTTAACCTCC 
CTGCGGGTCA TCCGAACGGA CTTCGGGGAC CCGGCCGTGG TGCTGTTTGA GGCCATGGAC 
TTCGAGGGGC CGGGCGTGGA GGTGAGCGAG GCACTGCCAG ACGTGGAGCT GGCGGGACAC 
GGCCCCAGAA CGCAGGCGAT CCACGTTCTC AGCGGCGTGT GGGTGGCGTA TCAGGAGGTG 
GGCTTCTCCG GGGAGCAGTA CGTGCTGGAG AAGGGCGTGT ACCGCAACTG CGACGACTGG 
GGCTCGGGCA ACAGCGCCCT CGCCTCGCTG CAGCCCGTCC TGCAGGTCGG GGAGCACAAT 
CTGCACTTTA TCTCAAAGAT TCAGCTTTTC TCCGGCCCCG ACTTCCTGGG TGACCACATC 
TCTTTTGAAG ATGACCAGAG CTCTCTGCCC ACCTCCTTCC AGCCCCAGTC TTGCCGAGTC 
CACGGCGGCA GCTGGATCCT GTTTGATGAG AAGAATTTTG AGGGTGAGCA GTACATTCTG 
TCTGAGGGCG AGTTCCCTAC TCTTACAGCC ATGGGCTGCC TGGCCTCCAC CGTCCTGGGC 
TCTCTCCGGA AGGTGCCCCT GTACTTTTCC GAGCCATCCA TTTTCCTGTA TGGACTGGAG 
TGCTTCGAGG GCAAGGAGAT TGAGCTCAGC GGGGAGGTGC GGAGCCTGCA GGCCGAGGGC 
TTCAACAATC ATGTGCTGTC CGTGCGGATC AAGGGGGGCG TCTGGGTGCT ATGCGAGCAC 
AGCGACTTCC GGGGCCGCCA GTGGCTGGTG GGCAGCTGTG AGATCACCAA CTGGCTGACA 
TACAGCGGGA CCCAGCGGGT GGGCTCCCTC TACCCCATCA AGCAGCGCCG GGCTTATTTT 
CGCCTCTGGA ATGCGGCACT GGGGGGATTC CTGACTGTGC CAGACCACGT GGAAGACATG 
AAGGCGGGCC GCGTGGTGGT CTCTGAGCCC CAAGCCGGAG GCAGCAACAT CTGGTTCTAC 
GAGGATGGGC TACTGAAGAA CCAGGTGGCC CCCACTATGA GCCTGCAGGT GATTGGCCCC 
CCCAGCACAG GCTCCAAGGT GGTGCTGTGG GCTGAGAGCC GCCTGCCCCG CCAGACATGG 
AGCATCAGTG AGTTGGGCTA CATCTGCAGC CACATGTTTG AAGGTCGGAT CCTAGATGTG 
AAAGGTGGCC GGGGCTACGA CCGGGACCAC GCAGTGCTGT GGGAGCTGGC CAAGGACAGG 
GCGTCCCAGA TCTGGACTGT CCATGTGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315569.1
CDS	246..5558
Translation	MGPESLAPAVTSGEPAGARGPSGPPPVPGCLEQGPPGHTCQQPPHSARGFPLWPKAPSNLEDVLPHPASCPRAEGAKGEPTTSGPALPEPTLPEPAQTLRERPSARRTRYYRITVSLQGREQAPGEETEEPKPAQPAPHPCGPEESRGWQEPPQGPRPITGCLADPPQEPQLRAPPHQGSTVQQQELRAGRTPGSPHRRELDHSEPRTPQDALPGPNMEEASGPPAEAKARVVSTTLTWRQRPPAQDEVKHRFHKVSLVSGAQSEAPSEETSESSHRREEVNGFAVREEETVNHQGPRDGAGSKSFQSHGPIFSKKYTLPLKEKRPVGRLKEAAEQGDGSPQPPRTEPPSAGVTARTELSVPAHGPREPSPHPGVSFTSGSSRSLEERRVTRTVRTTVMVGGHVDRRVNSTVINSVGPALVGEALPRGRNAARAVRAVVVNPRAEGSPSRSQALELLSSLVPTERTAPTSRLPRPLAAVPRSPDLGSTEGSALRQLPETRTAEPKDRSAPGPAGFLEEAHPTPSQEVPAAHPDQEQDRTLGAGVHEVPRVQRPASPTQSPLQTSQGHTPVPSSPRLQTRTLSLDLVQPPEQPEAPTQARAQLTLKPQGPQALPSIKREGLSDPPAATVLPMVKTEVVIVPGQSLAPSSMRWKAITSPGGLSAPSSPKNKVVQDSEKVPVVSLTQKEIVQGPGVPADSSPNQKEVVQGRSAPAASGPKPTNVAQGPEVSLISQKEVVWSTAGSPVPPLPKEEAAQGLTIPAVPSPKQEKVVQGSEESIVSSPTQKEVVQDPDASNVPSSTQKVAVQAPATLIAPSSSVGKVSSGPGGPPAPVMMGAETSQESQLVPEATKDKTFLEPSREEEEVALTADLEIFLDTLRSMEPPEILRTHRLPRAPRSSYLAMYATLPAIEEDQPGPCVLGPSPQEVPALEVKKEEKAQEEEEEEPENPYLSDDEKLQRRQERARLGPPRDPSPARPTQVSCSPLEMMKKHVAGAKGPHPEPGPEWQAGSRPTSRLGGSLLFGGLVPALKEAPTSEPLGTKLSALQPHRAPGLKKGPGQLPLLYSERPPPEKPARARPPEGWSPALKTQGKLNTRPGKIILFSEPGCQGSRREVWEDADDASGWARVASVRVVRGCWVLHEQPGFQGQKLVLPQGDMELGAPGSAWSSQGIGSLRRVVRDYITPEISLYSEEGLKGEQVKLTEALEDPQGLERPLQMASATVSAGLWLLYPKPFFEDTPCILEPGEYPTSEAWGASDPSMGSLKPMRLGCPSVERPGEPKAVVYEAPGFQGRSWEVNRDIYNLQQPEDSQSPNLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYTDWSRWGGYNEVLTSLRVIRTDFGDPAVVLFEAMDFEGPGVEVSEALPDVELAGHGPRTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCDDWGSGNSALASLQPVLQVGEHNLHFISKIQLFSGPDFLGDHISFEDDQSSLPTSFQPQSCRVHGGSWILFDEKNFEGEQYILSEGEFPTLTAMGCLASTVLGSLRKVPLYFSEPSIFLYGLECFEGKEIELSGEVRSLQAEGFNNHVLSVRIKGGVWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRAYFRLWNAALGGFLTVPDHVEDMKAGRVVVSEPQAGGSNIWFYEDGLLKNQVAPTMSLQVIGPPSTGSKVVLWAESRLPRQTWSISELGYICSHMFEGRILDVKGGRGYDRDHAVLWELAKDRASQIWTVHVL

Target ORF information:

RefSeq Version	XM_015460083.2
Organism	Bos taurus(cattle)
Definition	Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460083.2

ORF Insert Sequence:

ATGGGGCCGG AGAGCCTGGC ACCAGCGGTG ACCTCGGGAG AGCCTGCTGG AGCCAGGGGT 
CCCTCAGGCC CACCGCCGGT GCCTGGATGT TTGGAACAAG GACCTCCGGG CCACACCTGC 
CAGCAACCCC CACACTCAGC CCGGGGGTTT CCTCTGTGGC CCAAGGCACC CAGCAACCTG 
GAAGACGTGC TGCCCCACCC AGCGTCCTGC CCCAGGGCAG AGGGGGCAAA GGGCGAGCCT 
ACCACCTCAG GGCCGGCCCT GCCAGAGCCA ACCCTGCCAG AGCCAGCCCA GACCCTACGT 
GAGCGTCCCT CTGCCAGGAG GACCCGCTAC TACCGCATCA CTGTCAGCCT GCAGGGGCGT 
GAGCAAGCAC CTGGGGAGGA GACTGAAGAG CCCAAACCGG CCCAACCAGC TCCCCACCCC 
TGCGGCCCTG AGGAATCCAG GGGCTGGCAG GAGCCTCCTC AGGGGCCCCG CCCCATCACA 
GGGTGCCTAG CTGACCCGCC TCAGGAACCC CAGCTCCGAG CCCCACCACA TCAGGGGAGC 
ACGGTCCAGC AGCAGGAGCT CCGGGCCGGC CGGACGCCTG GGTCCCCGCA CAGACGCGAG 
CTGGACCACA GCGAGCCAAG GACACCTCAG GACGCCTTGC CAGGGCCCAA CATGGAGGAG 
GCCAGCGGGC CTCCGGCTGA GGCCAAGGCC CGAGTGGTGA GCACCACGCT GACATGGCGG 
CAGCGGCCCC CTGCCCAGGA CGAGGTCAAA CACCGTTTCC ACAAGGTGTC CCTGGTGTCA 
GGGGCCCAGT CGGAAGCCCC GTCCGAGGAG ACGTCTGAGT CCAGCCATCG TCGAGAGGAA 
GTCAATGGCT TTGCTGTCCG GGAGGAAGAG ACCGTGAACC ACCAGGGCCC CCGGGACGGG 
GCTGGCTCCA AGAGCTTCCA GAGCCACGGG CCCATCTTTT CCAAGAAGTA CACATTACCC 
CTCAAGGAGA AAAGGCCAGT GGGAAGACTG AAGGAGGCTG CGGAGCAGGG TGACGGCAGC 
CCCCAGCCCC CCAGGACCGA GCCGCCCAGC GCGGGAGTCA CGGCCAGGAC TGAGTTGTCG 
GTGCCAGCAC ACGGGCCCCG GGAGCCGAGC CCCCACCCGG GTGTGAGTTT CACCAGCGGG 
AGCTCCAGGA GCCTGGAGGA GCGGCGGGTG ACACGCACTG TGCGGACCAC CGTGATGGTG 
GGAGGGCACG TGGACCGCCG GGTGAACAGC ACCGTGATCA ACTCCGTGGG GCCCGCGCTC 
GTGGGCGAGG CCCTGCCAAG GGGCCGCAAT GCTGCCCGCG CGGTGCGGGC AGTGGTGGTG 
AACCCGCGGG CCGAGGGCTC CCCCAGTCGC AGTCAGGCCC TGGAGCTGCT AAGTAGCCTT 
GTGCCCACTG AGCGGACCGC CCCCACCAGC CGGCTTCCCA GGCCCCTGGC TGCTGTGCCA 
AGGAGTCCAG ATCTGGGCAG CACAGAAGGG TCAGCCCTGA GGCAGCTGCC TGAGACGAGG 
ACAGCAGAGC CGAAGGACAG ATCTGCCCCG GGCCCTGCGG GCTTCCTGGA GGAAGCTCAC 
CCCACTCCGA GCCAGGAGGT CCCTGCAGCC CACCCAGACC AGGAACAGGA CAGAACCCTG 
GGTGCCGGGG TCCATGAGGT CCCAAGGGTG CAGAGACCCG CCAGCCCCAC CCAGTCCCCT 
CTCCAGACCT CTCAGGGCCA CACCCCAGTA CCTTCCTCTC CCAGACTCCA GACCCGAACC 
CTCTCCCTGG ACCTAGTTCA ACCCCCAGAG CAACCTGAAG CACCTACTCA GGCTCGGGCC 
CAGCTCACTC TCAAGCCCCA GGGACCCCAG GCCCTGCCCT CCATCAAAAG AGAAGGGCTT 
TCAGATCCCC CTGCGGCCAC CGTCCTTCCT ATGGTGAAGA CTGAGGTTGT GATAGTCCCT 
GGACAATCTC TTGCTCCATC CTCTATGAGA TGGAAGGCCA TCACCAGCCC GGGAGGTCTT 
TCTGCTCCAT CCTCCCCAAA GAATAAGGTT GTTCAGGACT CTGAAAAAGT CCCTGTTGTC 
TCCCTTACCC AGAAAGAGAT AGTACAGGGC CCTGGAGTTC CTGCTGACTC ATCTCCCAAC 
CAAAAAGAGG TAGTGCAGGG TCGGAGTGCT CCTGCTGCCT CAGGCCCCAA ACCAACCAAC 
GTTGCCCAGG GCCCAGAAGT CAGCCTGATC AGCCAGAAAG AGGTCGTCTG GAGTACAGCA 
GGCAGCCCGG TCCCTCCCCT CCCCAAGGAA GAGGCTGCTC AAGGCCTGAC AATTCCTGCC 
GTCCCATCCC CCAAACAGGA AAAGGTTGTC CAGGGGTCTG AAGAAAGCAT CGTCTCATCT 
CCCACCCAAA AAGAAGTTGT CCAGGACCCT GATGCTTCTA ATGTCCCATC TTCCACCCAG 
AAAGTAGCTG TCCAGGCCCC AGCCACTCTC ATCGCCCCCT CCTCCTCAGT GGGCAAGGTA 
TCCTCTGGTC CAGGAGGTCC CCCAGCCCCG GTGATGATGG GAGCAGAGAC CAGCCAGGAG 
TCCCAGCTTG TCCCTGAAGC CACCAAGGAC AAGACATTCC TGGAGCCATC GAGGGAGGAG 
GAGGAGGTGG CCCTGACTGC TGACCTGGAG ATTTTCCTGG ATACCCTGCG GAGCATGGAG 
CCTCCTGAGA TCCTGCGCAC TCACCGGCTA CCTCGAGCCC CCCGCTCCTC CTACCTGGCC 
ATGTACGCCA CCCTGCCTGC CATCGAGGAG GACCAGCCTG GGCCATGTGT GCTGGGACCC 
AGCCCCCAGG AGGTGCCAGC CCTGGAAGTA AAGAAGGAGG AGAAGGCACA GGAGGAGGAG 
GAAGAAGAGC CAGAGAACCC CTACCTGAGC GACGATGAAA AGCTCCAGCG CAGGCAGGAG 
AGAGCCAGGC TAGGCCCCCC CAGGGACCCC TCCCCTGCCC GGCCCACCCA GGTCTCTTGC 
TCTCCTCTGG AGATGATGAA GAAGCATGTA GCAGGTGCCA AGGGCCCCCA CCCAGAACCT 
GGGCCAGAGT GGCAGGCAGG CAGCCGGCCC ACTTCCCGTC TTGGAGGCAG CCTCCTCTTT 
GGGGGTCTGG TGCCTGCCCT GAAGGAGGCC CCCACCTCGG AACCACTGGG CACAAAACTA 
TCCGCTCTGC AGCCCCACAG GGCACCGGGG CTCAAGAAGG GTCCAGGACA GCTGCCCCTG 
CTCTACAGTG AAAGGCCACC TCCAGAAAAG CCGGCACGTG CCCGGCCCCC GGAGGGGTGG 
AGCCCAGCAC TGAAGACCCA GGGCAAGCTG AACACGAGAC CTGGAAAGAT TATCCTCTTC 
TCAGAGCCTG GCTGCCAAGG CAGCCGCAGG GAGGTCTGGG AAGATGCTGA TGATGCTTCC 
GGCTGGGCCC GTGTGGCCTC CGTACGGGTG GTCCGAGGCT GCTGGGTGCT GCACGAACAG 
CCAGGATTCC AGGGCCAGAA GCTGGTCCTG CCTCAAGGGG ATATGGAACT TGGAGCCCCA 
GGATCAGCAT GGAGTAGCCA AGGCATCGGC TCCCTGAGGA GGGTCGTCCG GGATTACATT 
ACCCCAGAGA TCAGTCTGTA CTCTGAGGAG GGCCTCAAAG GGGAGCAAGT GAAGCTGACT 
GAGGCCTTGG AGGACCCCCA GGGCCTGGAG AGGCCCCTGC AAATGGCATC TGCCACTGTT 
TCTGCAGGAC TGTGGCTTCT ATACCCCAAA CCCTTCTTTG AAGATACTCC CTGTATTCTG 
GAGCCTGGAG AGTATCCCAC CTCAGAGGCC TGGGGAGCAT CAGACCCCAG CATGGGCTCC 
CTGAAGCCCA TGAGATTGGG CTGCCCAAGT GTGGAGAGGC CAGGGGAGCC CAAGGCTGTG 
GTGTATGAGG CCCCAGGGTT TCAGGGCCGG AGCTGGGAAG TGAACAGAGA CATCTACAAC 
CTGCAGCAGC CAGAGGATAG CCAGAGCCCC AACTTGGCGT CTGTGGGGTC CCTGCGAGTT 
CTCGGGGGCT GCTGGGTGGG CTACGAGAAG GAGGGTTTCC GGGGTCACCA GTATCTGCTG 
GAAGAGGGAG AATACACTGA TTGGTCACGT TGGGGAGGCT ATAACGAGGT GTTAACCTCC 
CTGCGGGTCA TCCGAACGGA CTTCGGGGAC CCGGCCGTGG TGCTGTTTGA GGCCATGGAC 
TTCGAGGGGC CGGGCGTGGA GGTGAGCGAG GCACTGCCAG ACGTGGAGCT GGCGGGACAC 
GGCCCCAGAA CGCAGGCGAT CCACGTTCTC AGCGGCGTGT GGGTGGCGTA TCAGGAGGTG 
GGCTTCTCCG GGGAGCAGTA CGTGCTGGAG AAGGGCGTGT ACCGCAACTG CGACGACTGG 
GGCTCGGGCA ACAGCGCCCT CGCCTCGCTG CAGCCCGTCC TGCAGGTCGG GGAGCACAAT 
CTGCACTTTA TCTCAAAGAT TCAGCTTTTC TCCGGCCCCG ACTTCCTGGG TGACCACATC 
TCTTTTGAAG ATGACCAGAG CTCTCTGCCC ACCTCCTTCC AGCCCCAGTC TTGCCGAGTC 
CACGGCGGCA GCTGGATCCT GTTTGATGAG AAGAATTTTG AGGGTGAGCA GTACATTCTG 
TCTGAGGGCG AGTTCCCTAC TCTTACAGCC ATGGGCTGCC TGGCCTCCAC CGTCCTGGGC 
TCTCTCCGGA AGGTGCCCCT GTACTTTTCC GAGCCATCCA TTTTCCTGTA TGGACTGGAG 
TGCTTCGAGG GCAAGGAGAT TGAGCTCAGC GGGGAGGTGC GGAGCCTGCA GGCCGAGGGC 
TTCAACAATC ATGTGCTGTC CGTGCGGATC AAGGGGGGCG TCTGGGTGCT ATGCGAGCAC 
AGCGACTTCC GGGGCCGCCA GTGGCTGGTG GGCAGCTGTG AGATCACCAA CTGGCTGACA 
TACAGCGGGA CCCAGCGGGT GGGCTCCCTC TACCCCATCA AGCAGCGCCG GGCTTATTTT 
CGCCTCTGGA ATGCGGCACT GGGGGGATTC CTGACTGTGC CAGACCACGT GGAAGACATG 
AAGGCGGGCC GCGTGGTGGT CTCTGAGCCC CAAGCCGGAG GCAGCAACAT CTGGTTCTAC 
GAGGATGGGC TACTGAAGAA CCAGGTGGCC CCCACTATGA GCCTGCAGGT GATTGGCCCC 
CCCAGCACAG GCTCCAAGGT GGTGCTGTGG GCTGAGAGCC GCCTGCCCCG CCAGACATGG 
AGCATCAGTG AGTTGGGCTA CATCTGCAGC CACATGTTTG AAGGTCGGAT CCTAGATGTG 
AAAGGTGGCC GGGGCTACGA CCGGGACCAC GCAGTGCTGT GGGAGCTGGC CAAGGACAGG 
GCGTCCCAGA TCTGGACTGT CCATGTGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG2 ( NM_001109791.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001103261.1 Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), mRNA.

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CloneID	OBa36922
Clone ID Related Accession (Same CDS sequence)	NM_001109791.1
Accession Version	NM_001109791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	beta/gamma crystallin domain-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149807.1. On Oct 9, 2007 this sequence version replaced XM_583700.3. ##Evidence-Data-START## Transcript exon combination :: BC149807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGG CCAGCGGGCC TCCGGCTGAG GCCAAGGCCC GAGTGGTGAG CACCACGCTG 
ACATGGCGGC AGCGGCCCCC TGCCCAGGAC GAGGTCAAAC ACCGTTTCCA CAAGGTGTCC 
CTGGTGTCAG GGGCCCAGTC GGAAGCCCCG TCCGAGGAGA CGTCTGAGTC CAGCCATCGT 
CGAGAGGAAG TCAATGGCTT TGCTGTCCGG GAGGAAGAGA CCGTGAACCA CCAGGGCCCC 
CGGGACGGGG CTGGCTCCAA GAGCTTCCAG AGCCACGGGC CCATCTTTTC CAAGAAGTAC 
ACATTACCCC TCAAGGAGAA AAGGCCAGTG GGAAGACTGA AGGAGGCTGC GGAGCAGGGT 
GACGGCAGCC CCCAGCCCCC CAGGACCGAG CCGCCCAGCG CGGGAGTCAC GGCCAGGACT 
GAGTTGTCGG TGCCAGCACA CGGGCCCCGG GAGCCGAGCC CCCACCCGGG TGTGAGTTTC 
ACCAGCGGGA GCTCCAGGAG CCTGGAGGAG CGGCGGGTGA CACGCACTGT GCGGACCACC 
GTGATGGTGG GAGGGCACGT GGACCGCCGG GTGAACAGCA CCGTGATCAA CTCCGTGGGG 
CCCGCGCTCG TGGGCGAGGC CCTGCCAAGG GGCCGCAATG CTGCCCGCGC GGTGCGGGCA 
GTGGTGGTGA ACCCGCGGGC CGAGGGCTCC CCCAGTCGCA GTCAGGCCCT GGAGCTGCTA 
AGTAGCCTTG TGCCCACTGA GCGGACCGCC CCCACCAGCC GGCTTCCCAG GCCCCTGGCT 
GCTGTGCCAA GGAGTCCAGA TCTGGGCAGC ACAGAAGGGT CAGCCCTGAG GCAGCTGCCT 
GAGACGAGGA CAGCAGAGCC GAAGGACAGA TCTGCCCCGG GCCCTGCGGG CTTCCTGGAG 
GAAGCTCACC CCACTCCGAG CCAGGAGGTC CCTGCAGCCC ACCCAGACCA GGAACAGGAC 
AGAACCCTGG GTGCCGGGGT CCATGAGGTC CCAAGGGTGC AGAGACCCGC CAGCCCCACC 
CAGTCCCCTC TCCAGACCTC TCAGGGCCAC ACCCCAGTAC CTTCCTCTCC CAGACTCCAG 
ACCCGAACCC TCTCCCTGGA CCTAGTTCAA CCCCCAGAGC AACCTGAAGC ACCTACTCAG 
GCTCGGGCCC AGCTCACTCT CAAGCCCCAG GGACCCCAGG CCCTGCCCTC CATCAAAAGA 
GAAGGGCTTT CAGATCCCCC TGCGGCCACC GTCCTTCCTA TGGTGAAGAC TGAGGTTGTG 
ATAGTCCCTG GACAATCTCT TGCTCCATCC TCTATGAGAT GGAAGGCCAT CACCAGCCCG 
GGAGGTCTTT CTGCTCCATC CTCCCCAAAG AATAAGGTTG TTCAGGACTC TGAAAAAGTC 
CCTGTTGTCT CCCTTACCCA GAAAGAGATA GTACAGGGCC CTGGAGTTCC TGCTGACTCA 
TCTCCCAACC AAAAAGAGGT AGTGCAGGGT CGGAGTGCTC CTGCTGCCTC AGGCCCCAAA 
CCAACCAACG TTGCCCAGGG CCCAGAAGTC AGCCTGATCA GCCAGAAAGA GGTCGTCTGG 
AGTACAGCAG GCAGCCCGGT CCCTCCCCTC CCCAAGGAAG AGGCTGCTCA AGGCCTGACA 
ATTCCTGCCG TCCCATCCCC CAAACAGGAA AAGGTTGTCC AGGGGTCTGA AGAAAGCATC 
GTCTCATCTC CCACCCAAAA AGAAGTTGTC CAGGACCCTG ATGCTTCTAA TGTCCCATCT 
TCCACCCAGA AAGTAGCTGT CCAGGCCCCA GCCACTCTCA TCGCCCCCTC CTCCTCAGTG 
GGCAAGGTAT CCTCTGGTCC AGGAGGTCCC CCAGCCCCGG TGATGATGGG AGCAGAGACC 
AGCCAGGAGT CCCAGCTTGT CCCTGAAGCC ACCAAGGACA AGACATTCCT GGAGCCATCG 
AGGGAGGAGG AGGAGGTGGC CCTGACTGCT GACCTGGAGA TTTTCCTGGA TACCCTGCGG 
AGCATGGAGC CTCCTGAGAT CCTGCGCACT CACCGGCTAC CTCGAGCCCC CCGCTCCTCC 
TACCTGGCCA TGTACGCCAC CCTGCCTGCC ATCGAGGAGG ACCAGCCTGG GCCATGTGTG 
CTGGGACCCA GCCCCCAGGA GGTGCCAGCC CTGGAAGTAA AGAAGGAGGA GAAGGCACAG 
GAGGAGGAGG AAGAAGAGCC AGAGAACCCC TACCTGAGCG ACGATGAAAA GCTCCAGCGC 
AGGCAGGAGA GAGCCAGGCT AGGCCCCCCC AGGGACCCCT CCCCTGCCCG GCCCACCCAG 
GTCTCTTGCT CTCCTCTGGA GATGATGAAG AAGCATGTAG CAGGTGCCAA GGGCCCCCAC 
CCAGAACCTG GGCCAGAGTG GCAGGCAGGC AGCCGGCCCA CTTCCCGTCT TGGAGGCAGC 
CTCCTCTTTG GGGGTCTGGT GCCTGCCCTG AAGGAGGCCC CCACCTCGGA ACCACTGGGC 
ACAAAACTAT CCGCTCTGCA GCCCCACAGG GCACCGGGGC TCAAGAAGGG TCCAGGACAG 
CTGCCCCTGC TCTACAGTGA AAGGCCACCT CCAGAAAAGC CGGCACGTGC CCGGCCCCCG 
GAGGGGTGGA GCCCAGCACT GAAGACCCAG GGCAAGCTGA ACACGAGACC TGGAAAGATT 
ATCCTCTTCT CAGAGCCTGG CTGCCAAGGC AGCCGCAGGG AGGTCTGGGA AGATGCTGAT 
GATGCTTCCG GCTGGGCCCG TGTGGCCTCC GTACGGGTGG TCCGAGGCTG CTGGGTGCTG 
CACGAACAGC CAGGATTCCA GGGCCAGAAG CTGGTCCTGC CTCAAGGGGA TATGGAACTT 
GGAGCCCCAG GATCAGCATG GAGTAGCCAA GGCATCGGCT CCCTGAGGAG GGTCGTCCGG 
GATTACATTA CCCCAGAGAT CAGTCTGTAC TCTGAGGAGG GCCTCAAAGG GGAGCAAGTG 
AAGCTGACTG AGGCCTTGGA GGACCCCCAG GGCCTGGAGA GGCCCCTGCA AATGGCATCT 
GCCACTGTTT CTGCAGGACT GTGGCTTCTA TACCCCAAAC CCTTCTTTGA AGATACTCCC 
TGTATTCTGG AGCCTGGAGA GTATCCCACC TCAGAGGCCT GGGGAGCATC AGACCCCAGC 
ATGGGCTCCC TGAAGCCCAT GAGATTGGGC TGCCCAAGTG TGGAGAGGCC AGGGGAGCCC 
AAGGCTGTGG TGTATGAGGC CCCAGGGTTT CAGGGCCGGA GCTGGGAAGT GAACAGAGAC 
ATCTACAACC TGCAGCAGCC AGAGGATAGC CAGAGCCCCA ACTTGGCGTC TGTGGGGTCC 
CTGCGAGTTC TCGGGGGCTG CTGGGTGGGC TACGAGAAGG AGGGTTTCCG GGGTCACCAG 
TATCTGCTGG AAGAGGGAGA ATACACTGAT TGGTCACGTT GGGGAGGCTA TAACGAGGTG 
TTAACCTCCC TGCGGGTCAT CCGAACGGAC TTCGGGGACC CGGCCGTGGT GCTGTTTGAG 
GCCATGGACT TCGAGGGGCC GGGCGTGGAG GTGAGCGAGG CACTGCCAGA CGTGGAGCTG 
GCGGGACACG GCCCCAGAAC GCAGGCGATC CACGTTCTCA GCGGCGTGTG GGTGGCGTAT 
CAGGAGGTGG GCTTCTCCGG GGAGCAGTAC GTGCTGGAGA AGGGCGTGTA CCGCAACTGC 
GACGACTGGG GCTCGGGCAA CAGCGCCCTC GCCTCGCTGC AGCCCGTCCT GCAGGTCGGG 
GAGCACAATC TGCACTTTAT CTCAAAGATT CAGCTTTTCT CCGGCCCCGA CTTCCTGGGT 
GACCACATCT CTTTTGAAGA TGACCAGAGC TCTCTGCCCA CCTCCTTCCA GCCCCAGTCT 
TGCCGAGTCC ACGGCGGCAG CTGGATCCTG TTTGATGAGA AGAATTTTGA GGGTGAGCAG 
TACATTCTGT CTGAGGGCGA GTTCCCTACT CTTACAGCCA TGGGCTGCCT GGCCTCCACC 
GTCCTGGGCT CTCTCCGGAA GGTGCCCCTG TACTTTTCCG AGCCATCCAT TTTCCTGTAT 
GGACTGGAGT GCTTCGAGGG CAAGGAGATT GAGCTCAGCG GGGAGGTGCG GAGCCTGCAG 
GCCGAGGGCT TCAACAATCA TGTGCTGTCC GTGCGGATCA AGGGGGGCGT CTGGGTGCTA 
TGCGAGCACA GCGACTTCCG GGGCCGCCAG TGGCTGGTGG GCAGCTGTGA GATCACCAAC 
TGGCTGACAT ACAGCGGGAC CCAGCGGGTG GGCTCCCTCT ACCCCATCAA GCAGCGCCGG 
GCTTATTTTC GCCTCTGGAA TGCGGCACTG GGGGGATTCC TGACTGTGCC AGACCACGTG 
GAAGACATGA AGGCGGGCCG CGTGGTGGTC TCTGAGCCCC AAGCCGGAGG CAGCAACATC 
TGGTTCTACG AGGATGGGCT ACTGAAGAAC CAGGTGGCCC CCACTATGAG CCTGCAGGTG 
ATTGGCCCCC CCAGCACAGG CTCCAAGGTG GTGCTGTGGG CTGAGAGCCG CCTGCCCCGC 
CAGACATGGA GCATCAGTGA GTTGGGCTAC ATCTGCAGCC ACATGTTTGA AGGTCGGATC 
CTAGATGTGA AAGGTGGCCG GGGCTACGAC CGGGACCACG CAGTGCTGTG GGAGCTGGCC 
AAGGACAGGG CGTCCCAGAT CTGGACTGTC CATGTGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103261.1
CDS	111..4772
Translation	MEEASGPPAEAKARVVSTTLTWRQRPPAQDEVKHRFHKVSLVSGAQSEAPSEETSESSHRREEVNGFAVREEETVNHQGPRDGAGSKSFQSHGPIFSKKYTLPLKEKRPVGRLKEAAEQGDGSPQPPRTEPPSAGVTARTELSVPAHGPREPSPHPGVSFTSGSSRSLEERRVTRTVRTTVMVGGHVDRRVNSTVINSVGPALVGEALPRGRNAARAVRAVVVNPRAEGSPSRSQALELLSSLVPTERTAPTSRLPRPLAAVPRSPDLGSTEGSALRQLPETRTAEPKDRSAPGPAGFLEEAHPTPSQEVPAAHPDQEQDRTLGAGVHEVPRVQRPASPTQSPLQTSQGHTPVPSSPRLQTRTLSLDLVQPPEQPEAPTQARAQLTLKPQGPQALPSIKREGLSDPPAATVLPMVKTEVVIVPGQSLAPSSMRWKAITSPGGLSAPSSPKNKVVQDSEKVPVVSLTQKEIVQGPGVPADSSPNQKEVVQGRSAPAASGPKPTNVAQGPEVSLISQKEVVWSTAGSPVPPLPKEEAAQGLTIPAVPSPKQEKVVQGSEESIVSSPTQKEVVQDPDASNVPSSTQKVAVQAPATLIAPSSSVGKVSSGPGGPPAPVMMGAETSQESQLVPEATKDKTFLEPSREEEEVALTADLEIFLDTLRSMEPPEILRTHRLPRAPRSSYLAMYATLPAIEEDQPGPCVLGPSPQEVPALEVKKEEKAQEEEEEEPENPYLSDDEKLQRRQERARLGPPRDPSPARPTQVSCSPLEMMKKHVAGAKGPHPEPGPEWQAGSRPTSRLGGSLLFGGLVPALKEAPTSEPLGTKLSALQPHRAPGLKKGPGQLPLLYSERPPPEKPARARPPEGWSPALKTQGKLNTRPGKIILFSEPGCQGSRREVWEDADDASGWARVASVRVVRGCWVLHEQPGFQGQKLVLPQGDMELGAPGSAWSSQGIGSLRRVVRDYITPEISLYSEEGLKGEQVKLTEALEDPQGLERPLQMASATVSAGLWLLYPKPFFEDTPCILEPGEYPTSEAWGASDPSMGSLKPMRLGCPSVERPGEPKAVVYEAPGFQGRSWEVNRDIYNLQQPEDSQSPNLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYTDWSRWGGYNEVLTSLRVIRTDFGDPAVVLFEAMDFEGPGVEVSEALPDVELAGHGPRTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCDDWGSGNSALASLQPVLQVGEHNLHFISKIQLFSGPDFLGDHISFEDDQSSLPTSFQPQSCRVHGGSWILFDEKNFEGEQYILSEGEFPTLTAMGCLASTVLGSLRKVPLYFSEPSIFLYGLECFEGKEIELSGEVRSLQAEGFNNHVLSVRIKGGVWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRAYFRLWNAALGGFLTVPDHVEDMKAGRVVVSEPQAGGSNIWFYEDGLLKNQVAPTMSLQVIGPPSTGSKVVLWAESRLPRQTWSISELGYICSHMFEGRILDVKGGRGYDRDHAVLWELAKDRASQIWTVHVL

Target ORF information:

RefSeq Version	NM_001109791.1
Organism	Bos taurus(cattle)
Definition	Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109791.1

ORF Insert Sequence:

ATGGAGGAGG CCAGCGGGCC TCCGGCTGAG GCCAAGGCCC GAGTGGTGAG CACCACGCTG 
ACATGGCGGC AGCGGCCCCC TGCCCAGGAC GAGGTCAAAC ACCGTTTCCA CAAGGTGTCC 
CTGGTGTCAG GGGCCCAGTC GGAAGCCCCG TCCGAGGAGA CGTCTGAGTC CAGCCATCGT 
CGAGAGGAAG TCAATGGCTT TGCTGTCCGG GAGGAAGAGA CCGTGAACCA CCAGGGCCCC 
CGGGACGGGG CTGGCTCCAA GAGCTTCCAG AGCCACGGGC CCATCTTTTC CAAGAAGTAC 
ACATTACCCC TCAAGGAGAA AAGGCCAGTG GGAAGACTGA AGGAGGCTGC GGAGCAGGGT 
GACGGCAGCC CCCAGCCCCC CAGGACCGAG CCGCCCAGCG CGGGAGTCAC GGCCAGGACT 
GAGTTGTCGG TGCCAGCACA CGGGCCCCGG GAGCCGAGCC CCCACCCGGG TGTGAGTTTC 
ACCAGCGGGA GCTCCAGGAG CCTGGAGGAG CGGCGGGTGA CACGCACTGT GCGGACCACC 
GTGATGGTGG GAGGGCACGT GGACCGCCGG GTGAACAGCA CCGTGATCAA CTCCGTGGGG 
CCCGCGCTCG TGGGCGAGGC CCTGCCAAGG GGCCGCAATG CTGCCCGCGC GGTGCGGGCA 
GTGGTGGTGA ACCCGCGGGC CGAGGGCTCC CCCAGTCGCA GTCAGGCCCT GGAGCTGCTA 
AGTAGCCTTG TGCCCACTGA GCGGACCGCC CCCACCAGCC GGCTTCCCAG GCCCCTGGCT 
GCTGTGCCAA GGAGTCCAGA TCTGGGCAGC ACAGAAGGGT CAGCCCTGAG GCAGCTGCCT 
GAGACGAGGA CAGCAGAGCC GAAGGACAGA TCTGCCCCGG GCCCTGCGGG CTTCCTGGAG 
GAAGCTCACC CCACTCCGAG CCAGGAGGTC CCTGCAGCCC ACCCAGACCA GGAACAGGAC 
AGAACCCTGG GTGCCGGGGT CCATGAGGTC CCAAGGGTGC AGAGACCCGC CAGCCCCACC 
CAGTCCCCTC TCCAGACCTC TCAGGGCCAC ACCCCAGTAC CTTCCTCTCC CAGACTCCAG 
ACCCGAACCC TCTCCCTGGA CCTAGTTCAA CCCCCAGAGC AACCTGAAGC ACCTACTCAG 
GCTCGGGCCC AGCTCACTCT CAAGCCCCAG GGACCCCAGG CCCTGCCCTC CATCAAAAGA 
GAAGGGCTTT CAGATCCCCC TGCGGCCACC GTCCTTCCTA TGGTGAAGAC TGAGGTTGTG 
ATAGTCCCTG GACAATCTCT TGCTCCATCC TCTATGAGAT GGAAGGCCAT CACCAGCCCG 
GGAGGTCTTT CTGCTCCATC CTCCCCAAAG AATAAGGTTG TTCAGGACTC TGAAAAAGTC 
CCTGTTGTCT CCCTTACCCA GAAAGAGATA GTACAGGGCC CTGGAGTTCC TGCTGACTCA 
TCTCCCAACC AAAAAGAGGT AGTGCAGGGT CGGAGTGCTC CTGCTGCCTC AGGCCCCAAA 
CCAACCAACG TTGCCCAGGG CCCAGAAGTC AGCCTGATCA GCCAGAAAGA GGTCGTCTGG 
AGTACAGCAG GCAGCCCGGT CCCTCCCCTC CCCAAGGAAG AGGCTGCTCA AGGCCTGACA 
ATTCCTGCCG TCCCATCCCC CAAACAGGAA AAGGTTGTCC AGGGGTCTGA AGAAAGCATC 
GTCTCATCTC CCACCCAAAA AGAAGTTGTC CAGGACCCTG ATGCTTCTAA TGTCCCATCT 
TCCACCCAGA AAGTAGCTGT CCAGGCCCCA GCCACTCTCA TCGCCCCCTC CTCCTCAGTG 
GGCAAGGTAT CCTCTGGTCC AGGAGGTCCC CCAGCCCCGG TGATGATGGG AGCAGAGACC 
AGCCAGGAGT CCCAGCTTGT CCCTGAAGCC ACCAAGGACA AGACATTCCT GGAGCCATCG 
AGGGAGGAGG AGGAGGTGGC CCTGACTGCT GACCTGGAGA TTTTCCTGGA TACCCTGCGG 
AGCATGGAGC CTCCTGAGAT CCTGCGCACT CACCGGCTAC CTCGAGCCCC CCGCTCCTCC 
TACCTGGCCA TGTACGCCAC CCTGCCTGCC ATCGAGGAGG ACCAGCCTGG GCCATGTGTG 
CTGGGACCCA GCCCCCAGGA GGTGCCAGCC CTGGAAGTAA AGAAGGAGGA GAAGGCACAG 
GAGGAGGAGG AAGAAGAGCC AGAGAACCCC TACCTGAGCG ACGATGAAAA GCTCCAGCGC 
AGGCAGGAGA GAGCCAGGCT AGGCCCCCCC AGGGACCCCT CCCCTGCCCG GCCCACCCAG 
GTCTCTTGCT CTCCTCTGGA GATGATGAAG AAGCATGTAG CAGGTGCCAA GGGCCCCCAC 
CCAGAACCTG GGCCAGAGTG GCAGGCAGGC AGCCGGCCCA CTTCCCGTCT TGGAGGCAGC 
CTCCTCTTTG GGGGTCTGGT GCCTGCCCTG AAGGAGGCCC CCACCTCGGA ACCACTGGGC 
ACAAAACTAT CCGCTCTGCA GCCCCACAGG GCACCGGGGC TCAAGAAGGG TCCAGGACAG 
CTGCCCCTGC TCTACAGTGA AAGGCCACCT CCAGAAAAGC CGGCACGTGC CCGGCCCCCG 
GAGGGGTGGA GCCCAGCACT GAAGACCCAG GGCAAGCTGA ACACGAGACC TGGAAAGATT 
ATCCTCTTCT CAGAGCCTGG CTGCCAAGGC AGCCGCAGGG AGGTCTGGGA AGATGCTGAT 
GATGCTTCCG GCTGGGCCCG TGTGGCCTCC GTACGGGTGG TCCGAGGCTG CTGGGTGCTG 
CACGAACAGC CAGGATTCCA GGGCCAGAAG CTGGTCCTGC CTCAAGGGGA TATGGAACTT 
GGAGCCCCAG GATCAGCATG GAGTAGCCAA GGCATCGGCT CCCTGAGGAG GGTCGTCCGG 
GATTACATTA CCCCAGAGAT CAGTCTGTAC TCTGAGGAGG GCCTCAAAGG GGAGCAAGTG 
AAGCTGACTG AGGCCTTGGA GGACCCCCAG GGCCTGGAGA GGCCCCTGCA AATGGCATCT 
GCCACTGTTT CTGCAGGACT GTGGCTTCTA TACCCCAAAC CCTTCTTTGA AGATACTCCC 
TGTATTCTGG AGCCTGGAGA GTATCCCACC TCAGAGGCCT GGGGAGCATC AGACCCCAGC 
ATGGGCTCCC TGAAGCCCAT GAGATTGGGC TGCCCAAGTG TGGAGAGGCC AGGGGAGCCC 
AAGGCTGTGG TGTATGAGGC CCCAGGGTTT CAGGGCCGGA GCTGGGAAGT GAACAGAGAC 
ATCTACAACC TGCAGCAGCC AGAGGATAGC CAGAGCCCCA ACTTGGCGTC TGTGGGGTCC 
CTGCGAGTTC TCGGGGGCTG CTGGGTGGGC TACGAGAAGG AGGGTTTCCG GGGTCACCAG 
TATCTGCTGG AAGAGGGAGA ATACACTGAT TGGTCACGTT GGGGAGGCTA TAACGAGGTG 
TTAACCTCCC TGCGGGTCAT CCGAACGGAC TTCGGGGACC CGGCCGTGGT GCTGTTTGAG 
GCCATGGACT TCGAGGGGCC GGGCGTGGAG GTGAGCGAGG CACTGCCAGA CGTGGAGCTG 
GCGGGACACG GCCCCAGAAC GCAGGCGATC CACGTTCTCA GCGGCGTGTG GGTGGCGTAT 
CAGGAGGTGG GCTTCTCCGG GGAGCAGTAC GTGCTGGAGA AGGGCGTGTA CCGCAACTGC 
GACGACTGGG GCTCGGGCAA CAGCGCCCTC GCCTCGCTGC AGCCCGTCCT GCAGGTCGGG 
GAGCACAATC TGCACTTTAT CTCAAAGATT CAGCTTTTCT CCGGCCCCGA CTTCCTGGGT 
GACCACATCT CTTTTGAAGA TGACCAGAGC TCTCTGCCCA CCTCCTTCCA GCCCCAGTCT 
TGCCGAGTCC ACGGCGGCAG CTGGATCCTG TTTGATGAGA AGAATTTTGA GGGTGAGCAG 
TACATTCTGT CTGAGGGCGA GTTCCCTACT CTTACAGCCA TGGGCTGCCT GGCCTCCACC 
GTCCTGGGCT CTCTCCGGAA GGTGCCCCTG TACTTTTCCG AGCCATCCAT TTTCCTGTAT 
GGACTGGAGT GCTTCGAGGG CAAGGAGATT GAGCTCAGCG GGGAGGTGCG GAGCCTGCAG 
GCCGAGGGCT TCAACAATCA TGTGCTGTCC GTGCGGATCA AGGGGGGCGT CTGGGTGCTA 
TGCGAGCACA GCGACTTCCG GGGCCGCCAG TGGCTGGTGG GCAGCTGTGA GATCACCAAC 
TGGCTGACAT ACAGCGGGAC CCAGCGGGTG GGCTCCCTCT ACCCCATCAA GCAGCGCCGG 
GCTTATTTTC GCCTCTGGAA TGCGGCACTG GGGGGATTCC TGACTGTGCC AGACCACGTG 
GAAGACATGA AGGCGGGCCG CGTGGTGGTC TCTGAGCCCC AAGCCGGAGG CAGCAACATC 
TGGTTCTACG AGGATGGGCT ACTGAAGAAC CAGGTGGCCC CCACTATGAG CCTGCAGGTG 
ATTGGCCCCC CCAGCACAGG CTCCAAGGTG GTGCTGTGGG CTGAGAGCCG CCTGCCCCGC 
CAGACATGGA GCATCAGTGA GTTGGGCTAC ATCTGCAGCC ACATGTTTGA AGGTCGGATC 
CTAGATGTGA AAGGTGGCCG GGGCTACGAC CGGGACCACG CAGTGCTGTG GGAGCTGGCC 
AAGGACAGGG CGTCCCAGAT CTGGACTGTC CATGTGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG2 ( XM_005203089.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203146.1 Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X2, mRNA.

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CloneID	OBa16903
Clone ID Related Accession (Same CDS sequence)	XM_005203089.3 , XM_005203089.4
Accession Version	XM_005203089.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	absent in melanoma 1-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGC GGCGCGGGGG CCTAGGGGCC CGCCTGGCTC GCCGCCTGGC CGGGCTGGGG 
GTCCGGAGGG AGCGCAAGGG GAAGCGGACC CCGGAGGAGG CCAGATCCCG GGATAGGCCC 
TGGGGTGGCC AGAGCTGCCC TGACCTGGCC CAGGGCCAGG GCAGCACCAG CAAAGTCAGC 
CTCAAGGCCC AAGCCCAGAG CCACTCAGAG TTGGACCTTC GTGCAGGGAG CCTTGGCTCC 
TGGCTTTGGA AGCGACGACA CAGCTCAGTG GGGCCCGTGG AGCCTCTGGG GCGCCTCCAG 
AGGCCTGCTG TAGGCAGTGC CTCTGACCTG GCCAACCATG CCTCCATGGG GCCGGAGAGC 
CTGGCACCAG CGGTGACCTC GGGAGAGCCT GCTGGAGCCA GGGGTCCCTC AGGCCCACCG 
CCGGTGCCTG GATGTTTGGA ACAAGGACCT CCGGGCCACA CCTGCCAGCA ACCCCCACAC 
TCAGCCCGGG GGTTTCCTCT GTGGCCCAAG GCACCCAGCA ACCTGGAAGA CGTGCTGCCC 
CACCCAGCGT CCTGCCCCAG GGCAGAGGGG GCAAAGGGCG AGCCTACCAC CTCAGGGCCG 
GCCCTGCCAG AGCCAACCCT GCCAGAGCCA GCCCAGACCC TACGTGAGCG TCCCTCTGCC 
AGGAGGACCC GCTACTACCG CATCACTGTC AGCCTGCAGG GGCGTGAGCA AGCACCTGGG 
GAGGAGACTG AAGAGCCCAA ACCGGCCCAA CCAGCTCCCC ACCCCTGCGG CCCTGAGGAA 
TCCAGGGGCT GGCAGGAGCC TCCTCAGGGG CCCCGCCCCA TCACAGGGTG CCTAGCTGAC 
CCGCCTCAGG AACCCCAGCT CCGAGCCCCA CCACATCAGG GGAGCACGGT CCAGCAGCAG 
GAGCTCCGGG CCGGCCGGAC GCCTGGGTCC CCGCACAGAC GCGAGCTGGA CCACAGCGAG 
CCAAGGACAC CTCAGGACGC CTTGCCAGGG CCCAACATGG AGGAGGCCAG CGGGCCTCCG 
GCTGAGGCCA AGGCCCGAGT GGTGAGCACC ACGCTGACAT GGCGGCAGCG GCCCCCTGCC 
CAGGACGAGG TCAAACACCG TTTCCACAAG GTGTCCCTGG TGTCAGGGGC CCAGTCGGAA 
GCCCCGTCCG AGGAGACGTC TGAGTCCAGC CATCGTCGAG AGGAAGTCAA TGGCTTTGCT 
GTCCGGGAGG AAGAGACCGT GAACCACCAG GGCCCCCGGG ACGGGGCTGG CTCCAAGAGC 
TTCCAGAGCC ACGGGCCCAT CTTTTCCAAG AAGTACACAT TACCCCTCAA GGAGAAAAGG 
CCAGTGGGAA GACTGAAGGA GGCTGCGGAG CAGGGTGACG GCAGCCCCCA GCCCCCCAGG 
ACCGAGCCGC CCAGCGCGGG AGTCACGGCC AGGACTGAGT TGTCGGTGCC AGCACACGGG 
CCCCGGGAGC CGAGCCCCCA CCCGGGTGTG AGTTTCACCA GCGGGAGCTC CAGGAGCCTG 
GAGGAGCGGC GGGTGACACG CACTGTGCGG ACCACCGTGA TGGTGGGAGG GCACGTGGAC 
CGCCGGGTGA ACAGCACCGT GATCAACTCC GTGGGGCCCG CGCTCGTGGG CGAGGCCCTG 
CCAAGGGGCC GCAATGCTGC CCGCGCGGTG CGGGCAGTGG TGGTGAACCC GCGGGCCGAG 
GGCTCCCCCA GTCGCAGTCA GGCCCTGGAG CTGCTAAGTA GCCTTGTGCC CACTGAGCGG 
ACCGCCCCCA CCAGCCGGCT TCCCAGGCCC CTGGCTGCTG TGCCAAGGAG TCCAGATCTG 
GGCAGCACAG AAGGGTCAGC CCTGAGGCAG CTGCCTGAGA CGAGGACAGC AGAGCCGAAG 
GACAGATCTG CCCCGGGCCC TGCGGGCTTC CTGGAGGAAG CTCACCCCAC TCCGAGCCAG 
GAGGTCCCTG CAGCCCACCC AGACCAGGAA CAGGACAGAA CCCTGGGTGC CGGGGTCCAT 
GAGGTCCCAA GGGTGCAGAG ACCCGCCAGC CCCACCCAGT CCCCTCTCCA GACCTCTCAG 
GGCCACACCC CAGTACCTTC CTCTCCCAGA CTCCAGACCC GAACCCTCTC CCTGGACCTA 
GTTCAACCCC CAGAGCAACC TGAAGCACCT ACTCAGGCTC GGGCCCAGCT CACTCTCAAG 
CCCCAGGGAC CCCAGGCCCT GCCCTCCATC AAAAGAGAAG GGCTTTCAGA TCCCCCTGCG 
GCCACCGTCC TTCCTATGGT GAAGACTGAG GTTGTGATAG TCCCTGGACA ATCTCTTGCT 
CCATCCTCTA TGAGATGGAA GGCCATCACC AGCCCGGGAG GTCTTTCTGC TCCATCCTCC 
CCAAAGAATA AGGTTGTTCA GGACTCTGAA AAAGTCCCTG TTGTCTCCCT TACCCAGAAA 
GAGATAGTAC AGGGCCCTGG AGTTCCTGCT GACTCATCTC CCAACCAAAA AGAGGTAGTG 
CAGGGTCGGA GTGCTCCTGC TGCCTCAGGC CCCAAACCAA CCAACGTTGC CCAGGGCCCA 
GAAGTCAGCC TGATCAGCCA GAAAGAGGTC GTCTGGAGTA CAGCAGGCAG CCCGGTCCCT 
CCCCTCCCCA AGGAAGAGGC TGCTCAAGGC CTGACAATTC CTGCCGTCCC ATCCCCCAAA 
CAGGAAAAGG TTGTCCAGGG GTCTGAAGAA AGCATCGTCT CATCTCCCAC CCAAAAAGAA 
GTTGTCCAGG ACCCTGATGC TTCTAATGTC CCATCTTCCA CCCAGAAAGT AGCTGTCCAG 
GCCCCAGCCA CTCTCATCGC CCCCTCCTCC TCAGTGGGCA AGGTATCCTC TGGTCCAGGA 
GGTCCCCCAG CCCCGGTGAT GATGGGAGCA GAGACCAGCC AGGAGTCCCA GCTTGTCCCT 
GAAGCCACCA AGGACAAGAC ATTCCTGGAG CCATCGAGGG AGGAGGAGGA GGTGGCCCTG 
ACTGCTGACC TGGAGATTTT CCTGGATACC CTGCGGAGCA TGGAGCCTCC TGAGATCCTG 
CGCACTCACC GGCTACCTCG AGCCCCCCGC TCCTCCTACC TGGCCATGTA CGCCACCCTG 
CCTGCCATCG AGGAGGACCA GCCTGGGCCA TGTGTGCTGG GACCCAGCCC CCAGGAGGTG 
CCAGCCCTGG AAGTAAAGAA GGAGGAGAAG GCACAGGAGG AGGAGGAAGA AGAGCCAGAG 
AACCCCTACC TGAGCGACGA TGAAAAGCTC CAGCGCAGGC AGGAGAGAGC CAGGCTAGGC 
CCCCCCAGGG ACCCCTCCCC TGCCCGGCCC ACCCAGGTCT CTTGCTCTCC TCTGGAGATG 
ATGAAGAAGC ATGTAGCAGG TGCCAAGGGC CCCCACCCAG AACCTGGGCC AGAGTGGCAG 
GCAGGCAGCC GGCCCACTTC CCGTCTTGGA GGCAGCCTCC TCTTTGGGGG TCTGGTGCCT 
GCCCTGAAGG AGGCCCCCAC CTCGGAACCA CTGGGCACAA AACTATCCGC TCTGCAGCCC 
CACAGGGCAC CGGGGCTCAA GAAGGGTCCA GGACAGCTGC CCCTGCTCTA CAGTGAAAGG 
CCACCTCCAG AAAAGCCGGC ACGTGCCCGG CCCCCGGAGG GGTGGAGCCC AGCACTGAAG 
ACCCAGGGCA AGCTGAACAC GAGACCTGGA AAGATTATCC TCTTCTCAGA GCCTGGCTGC 
CAAGGCAGCC GCAGGGAGGT CTGGGAAGAT GCTGATGATG CTTCCGGCTG GGCCCGTGTG 
GCCTCCGTAC GGGTGGTCCG AGGCTGCTGG GTGCTGCACG AACAGCCAGG ATTCCAGGGC 
CAGAAGCTGG TCCTGCCTCA AGGGGATATG GAACTTGGAG CCCCAGGATC AGCATGGAGT 
AGCCAAGGCA TCGGCTCCCT GAGGAGGGTC GTCCGGGATT ACATTACCCC AGAGATCAGT 
CTGTACTCTG AGGAGGGCCT CAAAGGGGAG CAAGTGAAGC TGACTGAGGC CTTGGAGGAC 
CCCCAGGGCC TGGAGAGGCC CCTGCAAATG GCATCTGCCA CTGTTTCTGC AGGACTGTGG 
CTTCTATACC CCAAACCCTT CTTTGAAGAT ACTCCCTGTA TTCTGGAGCC TGGAGAGTAT 
CCCACCTCAG AGGCCTGGGG AGCATCAGAC CCCAGCATGG GCTCCCTGAA GCCCATGAGA 
TTGGGCTGCC CAAGTGTGGA GAGGCCAGGG GAGCCCAAGG CTGTGGTGTA TGAGGCCCCA 
GGGTTTCAGG GCCGGAGCTG GGAAGTGAAC AGAGACATCT ACAACCTGCA GCAGCCAGAG 
GATAGCCAGA GCCCCAACTT GGCGTCTGTG GGGTCCCTGC GAGTTCTCGG GGGCTGCTGG 
GTGGGCTACG AGAAGGAGGG TTTCCGGGGT CACCAGTATC TGCTGGAAGA GGGAGAATAC 
ACTGATTGGT CACGTTGGGG AGGCTATAAC GAGGTGTTAA CCTCCCTGCG GGTCATCCGA 
ACGGACTTCG GGGACCCGGC CGTGGTGCTG TTTGAGGCCA TGGACTTCGA GGGGCCGGGC 
GTGGAGGTGA GCGAGGCACT GCCAGACGTG GAGCTGGCGG GACACGGCCC CAGAACGCAG 
GCGATCCACG TTCTCAGCGG CGTGTGGGTG GCGTATCAGG AGGTGGGCTT CTCCGGGGAG 
CAGTACGTGC TGGAGAAGGG CGTGTACCGC AACTGCGACG ACTGGGGCTC GGGCAACAGC 
GCCCTCGCCT CGCTGCAGCC CGTCCTGCAG GTCGGGGAGC ACAATCTGCA CTTTATCTCA 
AAGATTCAGC TTTTCTCCGG CCCCGACTTC CTGGGTGACC ACATCTCTTT TGAAGATGAC 
CAGAGCTCTC TGCCCACCTC CTTCCAGCCC CAGTCTTGCC GAGTCCACGG CGGCAGCTGG 
ATCCTGTTTG ATGAGAAGAA TTTTGAGGGT GAGCAGTACA TTCTGTCTGA GGGCGAGTTC 
CCTACTCTTA CAGCCATGGG CTGCCTGGCC TCCACCGTCC TGGGCTCTCT CCGGAAGGTG 
CCCCTGTACT TTTCCGAGCC ATCCATTTTC CTGTATGGAC TGGAGTGCTT CGAGGGCAAG 
GAGATTGAGC TCAGCGGGGA GGTGCGGAGC CTGCAGGCCG AGGGCTTCAA CAATCATGTG 
CTGTCCGTGC GGATCAAGGG GGGCGTCTGG GTGCTATGCG AGCACAGCGA CTTCCGGGGC 
CGCCAGTGGC TGGTGGGCAG CTGTGAGATC ACCAACTGGC TGACATACAG CGGGACCCAG 
CGGGTGGGCT CCCTCTACCC CATCAAGCAG CGCCGGGCTT ATTTTCGCCT CTGGAATGCG 
GCACTGGGGG GATTCCTGAC TGTGCCAGAC CACGTGGAAG ACATGAAGGC GGGCCGCGTG 
GTGGTCTCTG AGCCCCAAGC CGGAGGCAGC AACATCTGGT TCTACGAGGA TGGGCTACTG 
AAGAACCAGG TGGCCCCCAC TATGAGCCTG CAGGTGATTG GCCCCCCCAG CACAGGCTCC 
AAGGTGGTGC TGTGGGCTGA GAGCCGCCTG CCCCGCCAGA CATGGAGCAT CAGTGAGTTG 
GGCTACATCT GCAGCCACAT GTTTGAAGGT CGGATCCTAG ATGTGAAAGG TGGCCGGGGC 
TACGACCGGG ACCACGCAGT GCTGTGGGAG CTGGCCAAGG ACAGGGCGTC CCAGATCTGG 
ACTGTCCATG TGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203146.1
CDS	840..6497
Translation	MAERRGGLGARLARRLAGLGVRRERKGKRTPEEARSRDRPWGGQSCPDLAQGQGSTSKVSLKAQAQSHSELDLRAGSLGSWLWKRRHSSVGPVEPLGRLQRPAVGSASDLANHASMGPESLAPAVTSGEPAGARGPSGPPPVPGCLEQGPPGHTCQQPPHSARGFPLWPKAPSNLEDVLPHPASCPRAEGAKGEPTTSGPALPEPTLPEPAQTLRERPSARRTRYYRITVSLQGREQAPGEETEEPKPAQPAPHPCGPEESRGWQEPPQGPRPITGCLADPPQEPQLRAPPHQGSTVQQQELRAGRTPGSPHRRELDHSEPRTPQDALPGPNMEEASGPPAEAKARVVSTTLTWRQRPPAQDEVKHRFHKVSLVSGAQSEAPSEETSESSHRREEVNGFAVREEETVNHQGPRDGAGSKSFQSHGPIFSKKYTLPLKEKRPVGRLKEAAEQGDGSPQPPRTEPPSAGVTARTELSVPAHGPREPSPHPGVSFTSGSSRSLEERRVTRTVRTTVMVGGHVDRRVNSTVINSVGPALVGEALPRGRNAARAVRAVVVNPRAEGSPSRSQALELLSSLVPTERTAPTSRLPRPLAAVPRSPDLGSTEGSALRQLPETRTAEPKDRSAPGPAGFLEEAHPTPSQEVPAAHPDQEQDRTLGAGVHEVPRVQRPASPTQSPLQTSQGHTPVPSSPRLQTRTLSLDLVQPPEQPEAPTQARAQLTLKPQGPQALPSIKREGLSDPPAATVLPMVKTEVVIVPGQSLAPSSMRWKAITSPGGLSAPSSPKNKVVQDSEKVPVVSLTQKEIVQGPGVPADSSPNQKEVVQGRSAPAASGPKPTNVAQGPEVSLISQKEVVWSTAGSPVPPLPKEEAAQGLTIPAVPSPKQEKVVQGSEESIVSSPTQKEVVQDPDASNVPSSTQKVAVQAPATLIAPSSSVGKVSSGPGGPPAPVMMGAETSQESQLVPEATKDKTFLEPSREEEEVALTADLEIFLDTLRSMEPPEILRTHRLPRAPRSSYLAMYATLPAIEEDQPGPCVLGPSPQEVPALEVKKEEKAQEEEEEEPENPYLSDDEKLQRRQERARLGPPRDPSPARPTQVSCSPLEMMKKHVAGAKGPHPEPGPEWQAGSRPTSRLGGSLLFGGLVPALKEAPTSEPLGTKLSALQPHRAPGLKKGPGQLPLLYSERPPPEKPARARPPEGWSPALKTQGKLNTRPGKIILFSEPGCQGSRREVWEDADDASGWARVASVRVVRGCWVLHEQPGFQGQKLVLPQGDMELGAPGSAWSSQGIGSLRRVVRDYITPEISLYSEEGLKGEQVKLTEALEDPQGLERPLQMASATVSAGLWLLYPKPFFEDTPCILEPGEYPTSEAWGASDPSMGSLKPMRLGCPSVERPGEPKAVVYEAPGFQGRSWEVNRDIYNLQQPEDSQSPNLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYTDWSRWGGYNEVLTSLRVIRTDFGDPAVVLFEAMDFEGPGVEVSEALPDVELAGHGPRTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCDDWGSGNSALASLQPVLQVGEHNLHFISKIQLFSGPDFLGDHISFEDDQSSLPTSFQPQSCRVHGGSWILFDEKNFEGEQYILSEGEFPTLTAMGCLASTVLGSLRKVPLYFSEPSIFLYGLECFEGKEIELSGEVRSLQAEGFNNHVLSVRIKGGVWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRAYFRLWNAALGGFLTVPDHVEDMKAGRVVVSEPQAGGSNIWFYEDGLLKNQVAPTMSLQVIGPPSTGSKVVLWAESRLPRQTWSISELGYICSHMFEGRILDVKGGRGYDRDHAVLWELAKDRASQIWTVHVL

Target ORF information:

RefSeq Version	XM_005203089.3
Organism	Bos taurus(cattle)
Definition	Bos taurus absent in melanoma 1-like (AIM1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203089.3

ORF Insert Sequence:

ATGGCCGAGC GGCGCGGGGG CCTAGGGGCC CGCCTGGCTC GCCGCCTGGC CGGGCTGGGG 
GTCCGGAGGG AGCGCAAGGG GAAGCGGACC CCGGAGGAGG CCAGATCCCG GGATAGGCCC 
TGGGGTGGCC AGAGCTGCCC TGACCTGGCC CAGGGCCAGG GCAGCACCAG CAAAGTCAGC 
CTCAAGGCCC AAGCCCAGAG CCACTCAGAG TTGGACCTTC GTGCAGGGAG CCTTGGCTCC 
TGGCTTTGGA AGCGACGACA CAGCTCAGTG GGGCCCGTGG AGCCTCTGGG GCGCCTCCAG 
AGGCCTGCTG TAGGCAGTGC CTCTGACCTG GCCAACCATG CCTCCATGGG GCCGGAGAGC 
CTGGCACCAG CGGTGACCTC GGGAGAGCCT GCTGGAGCCA GGGGTCCCTC AGGCCCACCG 
CCGGTGCCTG GATGTTTGGA ACAAGGACCT CCGGGCCACA CCTGCCAGCA ACCCCCACAC 
TCAGCCCGGG GGTTTCCTCT GTGGCCCAAG GCACCCAGCA ACCTGGAAGA CGTGCTGCCC 
CACCCAGCGT CCTGCCCCAG GGCAGAGGGG GCAAAGGGCG AGCCTACCAC CTCAGGGCCG 
GCCCTGCCAG AGCCAACCCT GCCAGAGCCA GCCCAGACCC TACGTGAGCG TCCCTCTGCC 
AGGAGGACCC GCTACTACCG CATCACTGTC AGCCTGCAGG GGCGTGAGCA AGCACCTGGG 
GAGGAGACTG AAGAGCCCAA ACCGGCCCAA CCAGCTCCCC ACCCCTGCGG CCCTGAGGAA 
TCCAGGGGCT GGCAGGAGCC TCCTCAGGGG CCCCGCCCCA TCACAGGGTG CCTAGCTGAC 
CCGCCTCAGG AACCCCAGCT CCGAGCCCCA CCACATCAGG GGAGCACGGT CCAGCAGCAG 
GAGCTCCGGG CCGGCCGGAC GCCTGGGTCC CCGCACAGAC GCGAGCTGGA CCACAGCGAG 
CCAAGGACAC CTCAGGACGC CTTGCCAGGG CCCAACATGG AGGAGGCCAG CGGGCCTCCG 
GCTGAGGCCA AGGCCCGAGT GGTGAGCACC ACGCTGACAT GGCGGCAGCG GCCCCCTGCC 
CAGGACGAGG TCAAACACCG TTTCCACAAG GTGTCCCTGG TGTCAGGGGC CCAGTCGGAA 
GCCCCGTCCG AGGAGACGTC TGAGTCCAGC CATCGTCGAG AGGAAGTCAA TGGCTTTGCT 
GTCCGGGAGG AAGAGACCGT GAACCACCAG GGCCCCCGGG ACGGGGCTGG CTCCAAGAGC 
TTCCAGAGCC ACGGGCCCAT CTTTTCCAAG AAGTACACAT TACCCCTCAA GGAGAAAAGG 
CCAGTGGGAA GACTGAAGGA GGCTGCGGAG CAGGGTGACG GCAGCCCCCA GCCCCCCAGG 
ACCGAGCCGC CCAGCGCGGG AGTCACGGCC AGGACTGAGT TGTCGGTGCC AGCACACGGG 
CCCCGGGAGC CGAGCCCCCA CCCGGGTGTG AGTTTCACCA GCGGGAGCTC CAGGAGCCTG 
GAGGAGCGGC GGGTGACACG CACTGTGCGG ACCACCGTGA TGGTGGGAGG GCACGTGGAC 
CGCCGGGTGA ACAGCACCGT GATCAACTCC GTGGGGCCCG CGCTCGTGGG CGAGGCCCTG 
CCAAGGGGCC GCAATGCTGC CCGCGCGGTG CGGGCAGTGG TGGTGAACCC GCGGGCCGAG 
GGCTCCCCCA GTCGCAGTCA GGCCCTGGAG CTGCTAAGTA GCCTTGTGCC CACTGAGCGG 
ACCGCCCCCA CCAGCCGGCT TCCCAGGCCC CTGGCTGCTG TGCCAAGGAG TCCAGATCTG 
GGCAGCACAG AAGGGTCAGC CCTGAGGCAG CTGCCTGAGA CGAGGACAGC AGAGCCGAAG 
GACAGATCTG CCCCGGGCCC TGCGGGCTTC CTGGAGGAAG CTCACCCCAC TCCGAGCCAG 
GAGGTCCCTG CAGCCCACCC AGACCAGGAA CAGGACAGAA CCCTGGGTGC CGGGGTCCAT 
GAGGTCCCAA GGGTGCAGAG ACCCGCCAGC CCCACCCAGT CCCCTCTCCA GACCTCTCAG 
GGCCACACCC CAGTACCTTC CTCTCCCAGA CTCCAGACCC GAACCCTCTC CCTGGACCTA 
GTTCAACCCC CAGAGCAACC TGAAGCACCT ACTCAGGCTC GGGCCCAGCT CACTCTCAAG 
CCCCAGGGAC CCCAGGCCCT GCCCTCCATC AAAAGAGAAG GGCTTTCAGA TCCCCCTGCG 
GCCACCGTCC TTCCTATGGT GAAGACTGAG GTTGTGATAG TCCCTGGACA ATCTCTTGCT 
CCATCCTCTA TGAGATGGAA GGCCATCACC AGCCCGGGAG GTCTTTCTGC TCCATCCTCC 
CCAAAGAATA AGGTTGTTCA GGACTCTGAA AAAGTCCCTG TTGTCTCCCT TACCCAGAAA 
GAGATAGTAC AGGGCCCTGG AGTTCCTGCT GACTCATCTC CCAACCAAAA AGAGGTAGTG 
CAGGGTCGGA GTGCTCCTGC TGCCTCAGGC CCCAAACCAA CCAACGTTGC CCAGGGCCCA 
GAAGTCAGCC TGATCAGCCA GAAAGAGGTC GTCTGGAGTA CAGCAGGCAG CCCGGTCCCT 
CCCCTCCCCA AGGAAGAGGC TGCTCAAGGC CTGACAATTC CTGCCGTCCC ATCCCCCAAA 
CAGGAAAAGG TTGTCCAGGG GTCTGAAGAA AGCATCGTCT CATCTCCCAC CCAAAAAGAA 
GTTGTCCAGG ACCCTGATGC TTCTAATGTC CCATCTTCCA CCCAGAAAGT AGCTGTCCAG 
GCCCCAGCCA CTCTCATCGC CCCCTCCTCC TCAGTGGGCA AGGTATCCTC TGGTCCAGGA 
GGTCCCCCAG CCCCGGTGAT GATGGGAGCA GAGACCAGCC AGGAGTCCCA GCTTGTCCCT 
GAAGCCACCA AGGACAAGAC ATTCCTGGAG CCATCGAGGG AGGAGGAGGA GGTGGCCCTG 
ACTGCTGACC TGGAGATTTT CCTGGATACC CTGCGGAGCA TGGAGCCTCC TGAGATCCTG 
CGCACTCACC GGCTACCTCG AGCCCCCCGC TCCTCCTACC TGGCCATGTA CGCCACCCTG 
CCTGCCATCG AGGAGGACCA GCCTGGGCCA TGTGTGCTGG GACCCAGCCC CCAGGAGGTG 
CCAGCCCTGG AAGTAAAGAA GGAGGAGAAG GCACAGGAGG AGGAGGAAGA AGAGCCAGAG 
AACCCCTACC TGAGCGACGA TGAAAAGCTC CAGCGCAGGC AGGAGAGAGC CAGGCTAGGC 
CCCCCCAGGG ACCCCTCCCC TGCCCGGCCC ACCCAGGTCT CTTGCTCTCC TCTGGAGATG 
ATGAAGAAGC ATGTAGCAGG TGCCAAGGGC CCCCACCCAG AACCTGGGCC AGAGTGGCAG 
GCAGGCAGCC GGCCCACTTC CCGTCTTGGA GGCAGCCTCC TCTTTGGGGG TCTGGTGCCT 
GCCCTGAAGG AGGCCCCCAC CTCGGAACCA CTGGGCACAA AACTATCCGC TCTGCAGCCC 
CACAGGGCAC CGGGGCTCAA GAAGGGTCCA GGACAGCTGC CCCTGCTCTA CAGTGAAAGG 
CCACCTCCAG AAAAGCCGGC ACGTGCCCGG CCCCCGGAGG GGTGGAGCCC AGCACTGAAG 
ACCCAGGGCA AGCTGAACAC GAGACCTGGA AAGATTATCC TCTTCTCAGA GCCTGGCTGC 
CAAGGCAGCC GCAGGGAGGT CTGGGAAGAT GCTGATGATG CTTCCGGCTG GGCCCGTGTG 
GCCTCCGTAC GGGTGGTCCG AGGCTGCTGG GTGCTGCACG AACAGCCAGG ATTCCAGGGC 
CAGAAGCTGG TCCTGCCTCA AGGGGATATG GAACTTGGAG CCCCAGGATC AGCATGGAGT 
AGCCAAGGCA TCGGCTCCCT GAGGAGGGTC GTCCGGGATT ACATTACCCC AGAGATCAGT 
CTGTACTCTG AGGAGGGCCT CAAAGGGGAG CAAGTGAAGC TGACTGAGGC CTTGGAGGAC 
CCCCAGGGCC TGGAGAGGCC CCTGCAAATG GCATCTGCCA CTGTTTCTGC AGGACTGTGG 
CTTCTATACC CCAAACCCTT CTTTGAAGAT ACTCCCTGTA TTCTGGAGCC TGGAGAGTAT 
CCCACCTCAG AGGCCTGGGG AGCATCAGAC CCCAGCATGG GCTCCCTGAA GCCCATGAGA 
TTGGGCTGCC CAAGTGTGGA GAGGCCAGGG GAGCCCAAGG CTGTGGTGTA TGAGGCCCCA 
GGGTTTCAGG GCCGGAGCTG GGAAGTGAAC AGAGACATCT ACAACCTGCA GCAGCCAGAG 
GATAGCCAGA GCCCCAACTT GGCGTCTGTG GGGTCCCTGC GAGTTCTCGG GGGCTGCTGG 
GTGGGCTACG AGAAGGAGGG TTTCCGGGGT CACCAGTATC TGCTGGAAGA GGGAGAATAC 
ACTGATTGGT CACGTTGGGG AGGCTATAAC GAGGTGTTAA CCTCCCTGCG GGTCATCCGA 
ACGGACTTCG GGGACCCGGC CGTGGTGCTG TTTGAGGCCA TGGACTTCGA GGGGCCGGGC 
GTGGAGGTGA GCGAGGCACT GCCAGACGTG GAGCTGGCGG GACACGGCCC CAGAACGCAG 
GCGATCCACG TTCTCAGCGG CGTGTGGGTG GCGTATCAGG AGGTGGGCTT CTCCGGGGAG 
CAGTACGTGC TGGAGAAGGG CGTGTACCGC AACTGCGACG ACTGGGGCTC GGGCAACAGC 
GCCCTCGCCT CGCTGCAGCC CGTCCTGCAG GTCGGGGAGC ACAATCTGCA CTTTATCTCA 
AAGATTCAGC TTTTCTCCGG CCCCGACTTC CTGGGTGACC ACATCTCTTT TGAAGATGAC 
CAGAGCTCTC TGCCCACCTC CTTCCAGCCC CAGTCTTGCC GAGTCCACGG CGGCAGCTGG 
ATCCTGTTTG ATGAGAAGAA TTTTGAGGGT GAGCAGTACA TTCTGTCTGA GGGCGAGTTC 
CCTACTCTTA CAGCCATGGG CTGCCTGGCC TCCACCGTCC TGGGCTCTCT CCGGAAGGTG 
CCCCTGTACT TTTCCGAGCC ATCCATTTTC CTGTATGGAC TGGAGTGCTT CGAGGGCAAG 
GAGATTGAGC TCAGCGGGGA GGTGCGGAGC CTGCAGGCCG AGGGCTTCAA CAATCATGTG 
CTGTCCGTGC GGATCAAGGG GGGCGTCTGG GTGCTATGCG AGCACAGCGA CTTCCGGGGC 
CGCCAGTGGC TGGTGGGCAG CTGTGAGATC ACCAACTGGC TGACATACAG CGGGACCCAG 
CGGGTGGGCT CCCTCTACCC CATCAAGCAG CGCCGGGCTT ATTTTCGCCT CTGGAATGCG 
GCACTGGGGG GATTCCTGAC TGTGCCAGAC CACGTGGAAG ACATGAAGGC GGGCCGCGTG 
GTGGTCTCTG AGCCCCAAGC CGGAGGCAGC AACATCTGGT TCTACGAGGA TGGGCTACTG 
AAGAACCAGG TGGCCCCCAC TATGAGCCTG CAGGTGATTG GCCCCCCCAG CACAGGCTCC 
AAGGTGGTGC TGTGGGCTGA GAGCCGCCTG CCCCGCCAGA CATGGAGCAT CAGTGAGTTG 
GGCTACATCT GCAGCCACAT GTTTGAAGGT CGGATCCTAG ATGTGAAAGG TGGCCGGGGC 
TACGACCGGG ACCACGCAGT GCTGTGGGAG CTGGCCAAGG ACAGGGCGTC CCAGATCTGG 
ACTGTCCATG TGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG2 ( XM_005203089.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203146.1 Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), transcript variant X1, mRNA.

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CloneID	OBa16903
Clone ID Related Accession (Same CDS sequence)	XM_005203089.3 , XM_005203089.4
Accession Version	XM_005203089.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta/gamma crystallin domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203089.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGC GGCGCGGGGG CCTAGGGGCC CGCCTGGCTC GCCGCCTGGC CGGGCTGGGG 
GTCCGGAGGG AGCGCAAGGG GAAGCGGACC CCGGAGGAGG CCAGATCCCG GGATAGGCCC 
TGGGGTGGCC AGAGCTGCCC TGACCTGGCC CAGGGCCAGG GCAGCACCAG CAAAGTCAGC 
CTCAAGGCCC AAGCCCAGAG CCACTCAGAG TTGGACCTTC GTGCAGGGAG CCTTGGCTCC 
TGGCTTTGGA AGCGACGACA CAGCTCAGTG GGGCCCGTGG AGCCTCTGGG GCGCCTCCAG 
AGGCCTGCTG TAGGCAGTGC CTCTGACCTG GCCAACCATG CCTCCATGGG GCCGGAGAGC 
CTGGCACCAG CGGTGACCTC GGGAGAGCCT GCTGGAGCCA GGGGTCCCTC AGGCCCACCG 
CCGGTGCCTG GATGTTTGGA ACAAGGACCT CCGGGCCACA CCTGCCAGCA ACCCCCACAC 
TCAGCCCGGG GGTTTCCTCT GTGGCCCAAG GCACCCAGCA ACCTGGAAGA CGTGCTGCCC 
CACCCAGCGT CCTGCCCCAG GGCAGAGGGG GCAAAGGGCG AGCCTACCAC CTCAGGGCCG 
GCCCTGCCAG AGCCAACCCT GCCAGAGCCA GCCCAGACCC TACGTGAGCG TCCCTCTGCC 
AGGAGGACCC GCTACTACCG CATCACTGTC AGCCTGCAGG GGCGTGAGCA AGCACCTGGG 
GAGGAGACTG AAGAGCCCAA ACCGGCCCAA CCAGCTCCCC ACCCCTGCGG CCCTGAGGAA 
TCCAGGGGCT GGCAGGAGCC TCCTCAGGGG CCCCGCCCCA TCACAGGGTG CCTAGCTGAC 
CCGCCTCAGG AACCCCAGCT CCGAGCCCCA CCACATCAGG GGAGCACGGT CCAGCAGCAG 
GAGCTCCGGG CCGGCCGGAC GCCTGGGTCC CCGCACAGAC GCGAGCTGGA CCACAGCGAG 
CCAAGGACAC CTCAGGACGC CTTGCCAGGG CCCAACATGG AGGAGGCCAG CGGGCCTCCG 
GCTGAGGCCA AGGCCCGAGT GGTGAGCACC ACGCTGACAT GGCGGCAGCG GCCCCCTGCC 
CAGGACGAGG TCAAACACCG TTTCCACAAG GTGTCCCTGG TGTCAGGGGC CCAGTCGGAA 
GCCCCGTCCG AGGAGACGTC TGAGTCCAGC CATCGTCGAG AGGAAGTCAA TGGCTTTGCT 
GTCCGGGAGG AAGAGACCGT GAACCACCAG GGCCCCCGGG ACGGGGCTGG CTCCAAGAGC 
TTCCAGAGCC ACGGGCCCAT CTTTTCCAAG AAGTACACAT TACCCCTCAA GGAGAAAAGG 
CCAGTGGGAA GACTGAAGGA GGCTGCGGAG CAGGGTGACG GCAGCCCCCA GCCCCCCAGG 
ACCGAGCCGC CCAGCGCGGG AGTCACGGCC AGGACTGAGT TGTCGGTGCC AGCACACGGG 
CCCCGGGAGC CGAGCCCCCA CCCGGGTGTG AGTTTCACCA GCGGGAGCTC CAGGAGCCTG 
GAGGAGCGGC GGGTGACACG CACTGTGCGG ACCACCGTGA TGGTGGGAGG GCACGTGGAC 
CGCCGGGTGA ACAGCACCGT GATCAACTCC GTGGGGCCCG CGCTCGTGGG CGAGGCCCTG 
CCAAGGGGCC GCAATGCTGC CCGCGCGGTG CGGGCAGTGG TGGTGAACCC GCGGGCCGAG 
GGCTCCCCCA GTCGCAGTCA GGCCCTGGAG CTGCTAAGTA GCCTTGTGCC CACTGAGCGG 
ACCGCCCCCA CCAGCCGGCT TCCCAGGCCC CTGGCTGCTG TGCCAAGGAG TCCAGATCTG 
GGCAGCACAG AAGGGTCAGC CCTGAGGCAG CTGCCTGAGA CGAGGACAGC AGAGCCGAAG 
GACAGATCTG CCCCGGGCCC TGCGGGCTTC CTGGAGGAAG CTCACCCCAC TCCGAGCCAG 
GAGGTCCCTG CAGCCCACCC AGACCAGGAA CAGGACAGAA CCCTGGGTGC CGGGGTCCAT 
GAGGTCCCAA GGGTGCAGAG ACCCGCCAGC CCCACCCAGT CCCCTCTCCA GACCTCTCAG 
GGCCACACCC CAGTACCTTC CTCTCCCAGA CTCCAGACCC GAACCCTCTC CCTGGACCTA 
GTTCAACCCC CAGAGCAACC TGAAGCACCT ACTCAGGCTC GGGCCCAGCT CACTCTCAAG 
CCCCAGGGAC CCCAGGCCCT GCCCTCCATC AAAAGAGAAG GGCTTTCAGA TCCCCCTGCG 
GCCACCGTCC TTCCTATGGT GAAGACTGAG GTTGTGATAG TCCCTGGACA ATCTCTTGCT 
CCATCCTCTA TGAGATGGAA GGCCATCACC AGCCCGGGAG GTCTTTCTGC TCCATCCTCC 
CCAAAGAATA AGGTTGTTCA GGACTCTGAA AAAGTCCCTG TTGTCTCCCT TACCCAGAAA 
GAGATAGTAC AGGGCCCTGG AGTTCCTGCT GACTCATCTC CCAACCAAAA AGAGGTAGTG 
CAGGGTCGGA GTGCTCCTGC TGCCTCAGGC CCCAAACCAA CCAACGTTGC CCAGGGCCCA 
GAAGTCAGCC TGATCAGCCA GAAAGAGGTC GTCTGGAGTA CAGCAGGCAG CCCGGTCCCT 
CCCCTCCCCA AGGAAGAGGC TGCTCAAGGC CTGACAATTC CTGCCGTCCC ATCCCCCAAA 
CAGGAAAAGG TTGTCCAGGG GTCTGAAGAA AGCATCGTCT CATCTCCCAC CCAAAAAGAA 
GTTGTCCAGG ACCCTGATGC TTCTAATGTC CCATCTTCCA CCCAGAAAGT AGCTGTCCAG 
GCCCCAGCCA CTCTCATCGC CCCCTCCTCC TCAGTGGGCA AGGTATCCTC TGGTCCAGGA 
GGTCCCCCAG CCCCGGTGAT GATGGGAGCA GAGACCAGCC AGGAGTCCCA GCTTGTCCCT 
GAAGCCACCA AGGACAAGAC ATTCCTGGAG CCATCGAGGG AGGAGGAGGA GGTGGCCCTG 
ACTGCTGACC TGGAGATTTT CCTGGATACC CTGCGGAGCA TGGAGCCTCC TGAGATCCTG 
CGCACTCACC GGCTACCTCG AGCCCCCCGC TCCTCCTACC TGGCCATGTA CGCCACCCTG 
CCTGCCATCG AGGAGGACCA GCCTGGGCCA TGTGTGCTGG GACCCAGCCC CCAGGAGGTG 
CCAGCCCTGG AAGTAAAGAA GGAGGAGAAG GCACAGGAGG AGGAGGAAGA AGAGCCAGAG 
AACCCCTACC TGAGCGACGA TGAAAAGCTC CAGCGCAGGC AGGAGAGAGC CAGGCTAGGC 
CCCCCCAGGG ACCCCTCCCC TGCCCGGCCC ACCCAGGTCT CTTGCTCTCC TCTGGAGATG 
ATGAAGAAGC ATGTAGCAGG TGCCAAGGGC CCCCACCCAG AACCTGGGCC AGAGTGGCAG 
GCAGGCAGCC GGCCCACTTC CCGTCTTGGA GGCAGCCTCC TCTTTGGGGG TCTGGTGCCT 
GCCCTGAAGG AGGCCCCCAC CTCGGAACCA CTGGGCACAA AACTATCCGC TCTGCAGCCC 
CACAGGGCAC CGGGGCTCAA GAAGGGTCCA GGACAGCTGC CCCTGCTCTA CAGTGAAAGG 
CCACCTCCAG AAAAGCCGGC ACGTGCCCGG CCCCCGGAGG GGTGGAGCCC AGCACTGAAG 
ACCCAGGGCA AGCTGAACAC GAGACCTGGA AAGATTATCC TCTTCTCAGA GCCTGGCTGC 
CAAGGCAGCC GCAGGGAGGT CTGGGAAGAT GCTGATGATG CTTCCGGCTG GGCCCGTGTG 
GCCTCCGTAC GGGTGGTCCG AGGCTGCTGG GTGCTGCACG AACAGCCAGG ATTCCAGGGC 
CAGAAGCTGG TCCTGCCTCA AGGGGATATG GAACTTGGAG CCCCAGGATC AGCATGGAGT 
AGCCAAGGCA TCGGCTCCCT GAGGAGGGTC GTCCGGGATT ACATTACCCC AGAGATCAGT 
CTGTACTCTG AGGAGGGCCT CAAAGGGGAG CAAGTGAAGC TGACTGAGGC CTTGGAGGAC 
CCCCAGGGCC TGGAGAGGCC CCTGCAAATG GCATCTGCCA CTGTTTCTGC AGGACTGTGG 
CTTCTATACC CCAAACCCTT CTTTGAAGAT ACTCCCTGTA TTCTGGAGCC TGGAGAGTAT 
CCCACCTCAG AGGCCTGGGG AGCATCAGAC CCCAGCATGG GCTCCCTGAA GCCCATGAGA 
TTGGGCTGCC CAAGTGTGGA GAGGCCAGGG GAGCCCAAGG CTGTGGTGTA TGAGGCCCCA 
GGGTTTCAGG GCCGGAGCTG GGAAGTGAAC AGAGACATCT ACAACCTGCA GCAGCCAGAG 
GATAGCCAGA GCCCCAACTT GGCGTCTGTG GGGTCCCTGC GAGTTCTCGG GGGCTGCTGG 
GTGGGCTACG AGAAGGAGGG TTTCCGGGGT CACCAGTATC TGCTGGAAGA GGGAGAATAC 
ACTGATTGGT CACGTTGGGG AGGCTATAAC GAGGTGTTAA CCTCCCTGCG GGTCATCCGA 
ACGGACTTCG GGGACCCGGC CGTGGTGCTG TTTGAGGCCA TGGACTTCGA GGGGCCGGGC 
GTGGAGGTGA GCGAGGCACT GCCAGACGTG GAGCTGGCGG GACACGGCCC CAGAACGCAG 
GCGATCCACG TTCTCAGCGG CGTGTGGGTG GCGTATCAGG AGGTGGGCTT CTCCGGGGAG 
CAGTACGTGC TGGAGAAGGG CGTGTACCGC AACTGCGACG ACTGGGGCTC GGGCAACAGC 
GCCCTCGCCT CGCTGCAGCC CGTCCTGCAG GTCGGGGAGC ACAATCTGCA CTTTATCTCA 
AAGATTCAGC TTTTCTCCGG CCCCGACTTC CTGGGTGACC ACATCTCTTT TGAAGATGAC 
CAGAGCTCTC TGCCCACCTC CTTCCAGCCC CAGTCTTGCC GAGTCCACGG CGGCAGCTGG 
ATCCTGTTTG ATGAGAAGAA TTTTGAGGGT GAGCAGTACA TTCTGTCTGA GGGCGAGTTC 
CCTACTCTTA CAGCCATGGG CTGCCTGGCC TCCACCGTCC TGGGCTCTCT CCGGAAGGTG 
CCCCTGTACT TTTCCGAGCC ATCCATTTTC CTGTATGGAC TGGAGTGCTT CGAGGGCAAG 
GAGATTGAGC TCAGCGGGGA GGTGCGGAGC CTGCAGGCCG AGGGCTTCAA CAATCATGTG 
CTGTCCGTGC GGATCAAGGG GGGCGTCTGG GTGCTATGCG AGCACAGCGA CTTCCGGGGC 
CGCCAGTGGC TGGTGGGCAG CTGTGAGATC ACCAACTGGC TGACATACAG CGGGACCCAG 
CGGGTGGGCT CCCTCTACCC CATCAAGCAG CGCCGGGCTT ATTTTCGCCT CTGGAATGCG 
GCACTGGGGG GATTCCTGAC TGTGCCAGAC CACGTGGAAG ACATGAAGGC GGGCCGCGTG 
GTGGTCTCTG AGCCCCAAGC CGGAGGCAGC AACATCTGGT TCTACGAGGA TGGGCTACTG 
AAGAACCAGG TGGCCCCCAC TATGAGCCTG CAGGTGATTG GCCCCCCCAG CACAGGCTCC 
AAGGTGGTGC TGTGGGCTGA GAGCCGCCTG CCCCGCCAGA CATGGAGCAT CAGTGAGTTG 
GGCTACATCT GCAGCCACAT GTTTGAAGGT CGGATCCTAG ATGTGAAAGG TGGCCGGGGC 
TACGACCGGG ACCACGCAGT GCTGTGGGAG CTGGCCAAGG ACAGGGCGTC CCAGATCTGG 
ACTGTCCATG TGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203146.1
CDS	1122..6779
Translation	MAERRGGLGARLARRLAGLGVRRERKGKRTPEEARSRDRPWGGQSCPDLAQGQGSTSKVSLKAQAQSHSELDLRAGSLGSWLWKRRHSSVGPVEPLGRLQRPAVGSASDLANHASMGPESLAPAVTSGEPAGARGPSGPPPVPGCLEQGPPGHTCQQPPHSARGFPLWPKAPSNLEDVLPHPASCPRAEGAKGEPTTSGPALPEPTLPEPAQTLRERPSARRTRYYRITVSLQGREQAPGEETEEPKPAQPAPHPCGPEESRGWQEPPQGPRPITGCLADPPQEPQLRAPPHQGSTVQQQELRAGRTPGSPHRRELDHSEPRTPQDALPGPNMEEASGPPAEAKARVVSTTLTWRQRPPAQDEVKHRFHKVSLVSGAQSEAPSEETSESSHRREEVNGFAVREEETVNHQGPRDGAGSKSFQSHGPIFSKKYTLPLKEKRPVGRLKEAAEQGDGSPQPPRTEPPSAGVTARTELSVPAHGPREPSPHPGVSFTSGSSRSLEERRVTRTVRTTVMVGGHVDRRVNSTVINSVGPALVGEALPRGRNAARAVRAVVVNPRAEGSPSRSQALELLSSLVPTERTAPTSRLPRPLAAVPRSPDLGSTEGSALRQLPETRTAEPKDRSAPGPAGFLEEAHPTPSQEVPAAHPDQEQDRTLGAGVHEVPRVQRPASPTQSPLQTSQGHTPVPSSPRLQTRTLSLDLVQPPEQPEAPTQARAQLTLKPQGPQALPSIKREGLSDPPAATVLPMVKTEVVIVPGQSLAPSSMRWKAITSPGGLSAPSSPKNKVVQDSEKVPVVSLTQKEIVQGPGVPADSSPNQKEVVQGRSAPAASGPKPTNVAQGPEVSLISQKEVVWSTAGSPVPPLPKEEAAQGLTIPAVPSPKQEKVVQGSEESIVSSPTQKEVVQDPDASNVPSSTQKVAVQAPATLIAPSSSVGKVSSGPGGPPAPVMMGAETSQESQLVPEATKDKTFLEPSREEEEVALTADLEIFLDTLRSMEPPEILRTHRLPRAPRSSYLAMYATLPAIEEDQPGPCVLGPSPQEVPALEVKKEEKAQEEEEEEPENPYLSDDEKLQRRQERARLGPPRDPSPARPTQVSCSPLEMMKKHVAGAKGPHPEPGPEWQAGSRPTSRLGGSLLFGGLVPALKEAPTSEPLGTKLSALQPHRAPGLKKGPGQLPLLYSERPPPEKPARARPPEGWSPALKTQGKLNTRPGKIILFSEPGCQGSRREVWEDADDASGWARVASVRVVRGCWVLHEQPGFQGQKLVLPQGDMELGAPGSAWSSQGIGSLRRVVRDYITPEISLYSEEGLKGEQVKLTEALEDPQGLERPLQMASATVSAGLWLLYPKPFFEDTPCILEPGEYPTSEAWGASDPSMGSLKPMRLGCPSVERPGEPKAVVYEAPGFQGRSWEVNRDIYNLQQPEDSQSPNLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYTDWSRWGGYNEVLTSLRVIRTDFGDPAVVLFEAMDFEGPGVEVSEALPDVELAGHGPRTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCDDWGSGNSALASLQPVLQVGEHNLHFISKIQLFSGPDFLGDHISFEDDQSSLPTSFQPQSCRVHGGSWILFDEKNFEGEQYILSEGEFPTLTAMGCLASTVLGSLRKVPLYFSEPSIFLYGLECFEGKEIELSGEVRSLQAEGFNNHVLSVRIKGGVWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRAYFRLWNAALGGFLTVPDHVEDMKAGRVVVSEPQAGGSNIWFYEDGLLKNQVAPTMSLQVIGPPSTGSKVVLWAESRLPRQTWSISELGYICSHMFEGRILDVKGGRGYDRDHAVLWELAKDRASQIWTVHVL

Target ORF information:

RefSeq Version	XM_005203089.4
Organism	Bos taurus(cattle)
Definition	Bos taurus crystallin beta-gamma domain containing 2 (CRYBG2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203089.4

ORF Insert Sequence:

ATGGCCGAGC GGCGCGGGGG CCTAGGGGCC CGCCTGGCTC GCCGCCTGGC CGGGCTGGGG 
GTCCGGAGGG AGCGCAAGGG GAAGCGGACC CCGGAGGAGG CCAGATCCCG GGATAGGCCC 
TGGGGTGGCC AGAGCTGCCC TGACCTGGCC CAGGGCCAGG GCAGCACCAG CAAAGTCAGC 
CTCAAGGCCC AAGCCCAGAG CCACTCAGAG TTGGACCTTC GTGCAGGGAG CCTTGGCTCC 
TGGCTTTGGA AGCGACGACA CAGCTCAGTG GGGCCCGTGG AGCCTCTGGG GCGCCTCCAG 
AGGCCTGCTG TAGGCAGTGC CTCTGACCTG GCCAACCATG CCTCCATGGG GCCGGAGAGC 
CTGGCACCAG CGGTGACCTC GGGAGAGCCT GCTGGAGCCA GGGGTCCCTC AGGCCCACCG 
CCGGTGCCTG GATGTTTGGA ACAAGGACCT CCGGGCCACA CCTGCCAGCA ACCCCCACAC 
TCAGCCCGGG GGTTTCCTCT GTGGCCCAAG GCACCCAGCA ACCTGGAAGA CGTGCTGCCC 
CACCCAGCGT CCTGCCCCAG GGCAGAGGGG GCAAAGGGCG AGCCTACCAC CTCAGGGCCG 
GCCCTGCCAG AGCCAACCCT GCCAGAGCCA GCCCAGACCC TACGTGAGCG TCCCTCTGCC 
AGGAGGACCC GCTACTACCG CATCACTGTC AGCCTGCAGG GGCGTGAGCA AGCACCTGGG 
GAGGAGACTG AAGAGCCCAA ACCGGCCCAA CCAGCTCCCC ACCCCTGCGG CCCTGAGGAA 
TCCAGGGGCT GGCAGGAGCC TCCTCAGGGG CCCCGCCCCA TCACAGGGTG CCTAGCTGAC 
CCGCCTCAGG AACCCCAGCT CCGAGCCCCA CCACATCAGG GGAGCACGGT CCAGCAGCAG 
GAGCTCCGGG CCGGCCGGAC GCCTGGGTCC CCGCACAGAC GCGAGCTGGA CCACAGCGAG 
CCAAGGACAC CTCAGGACGC CTTGCCAGGG CCCAACATGG AGGAGGCCAG CGGGCCTCCG 
GCTGAGGCCA AGGCCCGAGT GGTGAGCACC ACGCTGACAT GGCGGCAGCG GCCCCCTGCC 
CAGGACGAGG TCAAACACCG TTTCCACAAG GTGTCCCTGG TGTCAGGGGC CCAGTCGGAA 
GCCCCGTCCG AGGAGACGTC TGAGTCCAGC CATCGTCGAG AGGAAGTCAA TGGCTTTGCT 
GTCCGGGAGG AAGAGACCGT GAACCACCAG GGCCCCCGGG ACGGGGCTGG CTCCAAGAGC 
TTCCAGAGCC ACGGGCCCAT CTTTTCCAAG AAGTACACAT TACCCCTCAA GGAGAAAAGG 
CCAGTGGGAA GACTGAAGGA GGCTGCGGAG CAGGGTGACG GCAGCCCCCA GCCCCCCAGG 
ACCGAGCCGC CCAGCGCGGG AGTCACGGCC AGGACTGAGT TGTCGGTGCC AGCACACGGG 
CCCCGGGAGC CGAGCCCCCA CCCGGGTGTG AGTTTCACCA GCGGGAGCTC CAGGAGCCTG 
GAGGAGCGGC GGGTGACACG CACTGTGCGG ACCACCGTGA TGGTGGGAGG GCACGTGGAC 
CGCCGGGTGA ACAGCACCGT GATCAACTCC GTGGGGCCCG CGCTCGTGGG CGAGGCCCTG 
CCAAGGGGCC GCAATGCTGC CCGCGCGGTG CGGGCAGTGG TGGTGAACCC GCGGGCCGAG 
GGCTCCCCCA GTCGCAGTCA GGCCCTGGAG CTGCTAAGTA GCCTTGTGCC CACTGAGCGG 
ACCGCCCCCA CCAGCCGGCT TCCCAGGCCC CTGGCTGCTG TGCCAAGGAG TCCAGATCTG 
GGCAGCACAG AAGGGTCAGC CCTGAGGCAG CTGCCTGAGA CGAGGACAGC AGAGCCGAAG 
GACAGATCTG CCCCGGGCCC TGCGGGCTTC CTGGAGGAAG CTCACCCCAC TCCGAGCCAG 
GAGGTCCCTG CAGCCCACCC AGACCAGGAA CAGGACAGAA CCCTGGGTGC CGGGGTCCAT 
GAGGTCCCAA GGGTGCAGAG ACCCGCCAGC CCCACCCAGT CCCCTCTCCA GACCTCTCAG 
GGCCACACCC CAGTACCTTC CTCTCCCAGA CTCCAGACCC GAACCCTCTC CCTGGACCTA 
GTTCAACCCC CAGAGCAACC TGAAGCACCT ACTCAGGCTC GGGCCCAGCT CACTCTCAAG 
CCCCAGGGAC CCCAGGCCCT GCCCTCCATC AAAAGAGAAG GGCTTTCAGA TCCCCCTGCG 
GCCACCGTCC TTCCTATGGT GAAGACTGAG GTTGTGATAG TCCCTGGACA ATCTCTTGCT 
CCATCCTCTA TGAGATGGAA GGCCATCACC AGCCCGGGAG GTCTTTCTGC TCCATCCTCC 
CCAAAGAATA AGGTTGTTCA GGACTCTGAA AAAGTCCCTG TTGTCTCCCT TACCCAGAAA 
GAGATAGTAC AGGGCCCTGG AGTTCCTGCT GACTCATCTC CCAACCAAAA AGAGGTAGTG 
CAGGGTCGGA GTGCTCCTGC TGCCTCAGGC CCCAAACCAA CCAACGTTGC CCAGGGCCCA 
GAAGTCAGCC TGATCAGCCA GAAAGAGGTC GTCTGGAGTA CAGCAGGCAG CCCGGTCCCT 
CCCCTCCCCA AGGAAGAGGC TGCTCAAGGC CTGACAATTC CTGCCGTCCC ATCCCCCAAA 
CAGGAAAAGG TTGTCCAGGG GTCTGAAGAA AGCATCGTCT CATCTCCCAC CCAAAAAGAA 
GTTGTCCAGG ACCCTGATGC TTCTAATGTC CCATCTTCCA CCCAGAAAGT AGCTGTCCAG 
GCCCCAGCCA CTCTCATCGC CCCCTCCTCC TCAGTGGGCA AGGTATCCTC TGGTCCAGGA 
GGTCCCCCAG CCCCGGTGAT GATGGGAGCA GAGACCAGCC AGGAGTCCCA GCTTGTCCCT 
GAAGCCACCA AGGACAAGAC ATTCCTGGAG CCATCGAGGG AGGAGGAGGA GGTGGCCCTG 
ACTGCTGACC TGGAGATTTT CCTGGATACC CTGCGGAGCA TGGAGCCTCC TGAGATCCTG 
CGCACTCACC GGCTACCTCG AGCCCCCCGC TCCTCCTACC TGGCCATGTA CGCCACCCTG 
CCTGCCATCG AGGAGGACCA GCCTGGGCCA TGTGTGCTGG GACCCAGCCC CCAGGAGGTG 
CCAGCCCTGG AAGTAAAGAA GGAGGAGAAG GCACAGGAGG AGGAGGAAGA AGAGCCAGAG 
AACCCCTACC TGAGCGACGA TGAAAAGCTC CAGCGCAGGC AGGAGAGAGC CAGGCTAGGC 
CCCCCCAGGG ACCCCTCCCC TGCCCGGCCC ACCCAGGTCT CTTGCTCTCC TCTGGAGATG 
ATGAAGAAGC ATGTAGCAGG TGCCAAGGGC CCCCACCCAG AACCTGGGCC AGAGTGGCAG 
GCAGGCAGCC GGCCCACTTC CCGTCTTGGA GGCAGCCTCC TCTTTGGGGG TCTGGTGCCT 
GCCCTGAAGG AGGCCCCCAC CTCGGAACCA CTGGGCACAA AACTATCCGC TCTGCAGCCC 
CACAGGGCAC CGGGGCTCAA GAAGGGTCCA GGACAGCTGC CCCTGCTCTA CAGTGAAAGG 
CCACCTCCAG AAAAGCCGGC ACGTGCCCGG CCCCCGGAGG GGTGGAGCCC AGCACTGAAG 
ACCCAGGGCA AGCTGAACAC GAGACCTGGA AAGATTATCC TCTTCTCAGA GCCTGGCTGC 
CAAGGCAGCC GCAGGGAGGT CTGGGAAGAT GCTGATGATG CTTCCGGCTG GGCCCGTGTG 
GCCTCCGTAC GGGTGGTCCG AGGCTGCTGG GTGCTGCACG AACAGCCAGG ATTCCAGGGC 
CAGAAGCTGG TCCTGCCTCA AGGGGATATG GAACTTGGAG CCCCAGGATC AGCATGGAGT 
AGCCAAGGCA TCGGCTCCCT GAGGAGGGTC GTCCGGGATT ACATTACCCC AGAGATCAGT 
CTGTACTCTG AGGAGGGCCT CAAAGGGGAG CAAGTGAAGC TGACTGAGGC CTTGGAGGAC 
CCCCAGGGCC TGGAGAGGCC CCTGCAAATG GCATCTGCCA CTGTTTCTGC AGGACTGTGG 
CTTCTATACC CCAAACCCTT CTTTGAAGAT ACTCCCTGTA TTCTGGAGCC TGGAGAGTAT 
CCCACCTCAG AGGCCTGGGG AGCATCAGAC CCCAGCATGG GCTCCCTGAA GCCCATGAGA 
TTGGGCTGCC CAAGTGTGGA GAGGCCAGGG GAGCCCAAGG CTGTGGTGTA TGAGGCCCCA 
GGGTTTCAGG GCCGGAGCTG GGAAGTGAAC AGAGACATCT ACAACCTGCA GCAGCCAGAG 
GATAGCCAGA GCCCCAACTT GGCGTCTGTG GGGTCCCTGC GAGTTCTCGG GGGCTGCTGG 
GTGGGCTACG AGAAGGAGGG TTTCCGGGGT CACCAGTATC TGCTGGAAGA GGGAGAATAC 
ACTGATTGGT CACGTTGGGG AGGCTATAAC GAGGTGTTAA CCTCCCTGCG GGTCATCCGA 
ACGGACTTCG GGGACCCGGC CGTGGTGCTG TTTGAGGCCA TGGACTTCGA GGGGCCGGGC 
GTGGAGGTGA GCGAGGCACT GCCAGACGTG GAGCTGGCGG GACACGGCCC CAGAACGCAG 
GCGATCCACG TTCTCAGCGG CGTGTGGGTG GCGTATCAGG AGGTGGGCTT CTCCGGGGAG 
CAGTACGTGC TGGAGAAGGG CGTGTACCGC AACTGCGACG ACTGGGGCTC GGGCAACAGC 
GCCCTCGCCT CGCTGCAGCC CGTCCTGCAG GTCGGGGAGC ACAATCTGCA CTTTATCTCA 
AAGATTCAGC TTTTCTCCGG CCCCGACTTC CTGGGTGACC ACATCTCTTT TGAAGATGAC 
CAGAGCTCTC TGCCCACCTC CTTCCAGCCC CAGTCTTGCC GAGTCCACGG CGGCAGCTGG 
ATCCTGTTTG ATGAGAAGAA TTTTGAGGGT GAGCAGTACA TTCTGTCTGA GGGCGAGTTC 
CCTACTCTTA CAGCCATGGG CTGCCTGGCC TCCACCGTCC TGGGCTCTCT CCGGAAGGTG 
CCCCTGTACT TTTCCGAGCC ATCCATTTTC CTGTATGGAC TGGAGTGCTT CGAGGGCAAG 
GAGATTGAGC TCAGCGGGGA GGTGCGGAGC CTGCAGGCCG AGGGCTTCAA CAATCATGTG 
CTGTCCGTGC GGATCAAGGG GGGCGTCTGG GTGCTATGCG AGCACAGCGA CTTCCGGGGC 
CGCCAGTGGC TGGTGGGCAG CTGTGAGATC ACCAACTGGC TGACATACAG CGGGACCCAG 
CGGGTGGGCT CCCTCTACCC CATCAAGCAG CGCCGGGCTT ATTTTCGCCT CTGGAATGCG 
GCACTGGGGG GATTCCTGAC TGTGCCAGAC CACGTGGAAG ACATGAAGGC GGGCCGCGTG 
GTGGTCTCTG AGCCCCAAGC CGGAGGCAGC AACATCTGGT TCTACGAGGA TGGGCTACTG 
AAGAACCAGG TGGCCCCCAC TATGAGCCTG CAGGTGATTG GCCCCCCCAG CACAGGCTCC 
AAGGTGGTGC TGTGGGCTGA GAGCCGCCTG CCCCGCCAGA CATGGAGCAT CAGTGAGTTG 
GGCTACATCT GCAGCCACAT GTTTGAAGGT CGGATCCTAG ATGTGAAAGG TGGCCGGGGC 
TACGACCGGG ACCACGCAGT GCTGTGGGAG CTGGCCAAGG ACAGGGCGTC CCAGATCTGG 
ACTGTCCATG TGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CRYBG2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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