RALGAPA2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RALGAPA2
Entrez Gene ID	507082
Full Name	Ral GTPase activating protein catalytic alpha subunit 2
General protein information	Preferred Names Ral GTPase activating protein catalytic alpha subunit 2 Names ral GTPase-activating protein subunit alpha-2 akt substrate AS250
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015465971.1	XP_015321457.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_015465974.1	XP_015321460.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_010822475.2	XP_010820777.2	ral GTPase-activating protein subunit alpha-2 isoform X13
XM_015474044.1	XP_015329530.1	ral GTPase-activating protein subunit alpha-2 isoform X2
XM_015474050.1	XP_015329536.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474056.1	XP_015329542.1	ral GTPase-activating protein subunit alpha-2 isoform X4
XM_015465970.1	XP_015321456.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_015465979.1	XP_015321465.1	ral GTPase-activating protein subunit alpha-2 isoform X12
XM_015465978.1	XP_015321464.1	ral GTPase-activating protein subunit alpha-2 isoform X12
XM_015465980.1	XP_015321466.1	ral GTPase-activating protein subunit alpha-2 isoform X12
XM_010822479.2	XP_010820781.2	ral GTPase-activating protein subunit alpha-2 isoform X14
XM_015474060.1	XP_015329546.1	ral GTPase-activating protein subunit alpha-2 isoform X5
XM_025001042.1	XP_024856810.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_025001044.1	XP_024856812.1	ral GTPase-activating protein subunit alpha-2 isoform X5
XM_015465972.1	XP_015321458.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_015474046.1	XP_015329532.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474047.1	XP_015329533.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474052.1	XP_015329538.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474055.1	XP_015329541.1	ral GTPase-activating protein subunit alpha-2 isoform X4
XM_015474061.1	XP_015329547.1	ral GTPase-activating protein subunit alpha-2 isoform X5
XM_015474062.1	XP_015329548.1	ral GTPase-activating protein subunit alpha-2 isoform X6
XM_025001040.1	XP_024856808.1	ral GTPase-activating protein subunit alpha-2 isoform X1
XM_015465975.1	XP_015321461.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_010822472.2	XP_010820774.2	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_010822474.2	XP_010820776.2	ral GTPase-activating protein subunit alpha-2 isoform X13
XM_015474043.1	XP_015329529.1	ral GTPase-activating protein subunit alpha-2 isoform X1
XM_015474048.1	XP_015329534.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474054.1	XP_015329540.1	ral GTPase-activating protein subunit alpha-2 isoform X4
XM_015474058.1	XP_015329544.1	ral GTPase-activating protein subunit alpha-2 isoform X5
XM_015474063.1	XP_015329549.1	ral GTPase-activating protein subunit alpha-2 isoform X7
XM_025001045.1	XP_024856813.1	ral GTPase-activating protein subunit alpha-2 isoform X6
XM_015474051.1	XP_015329537.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474053.1	XP_015329539.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474057.1	XP_015329543.1	ral GTPase-activating protein subunit alpha-2 isoform X4
XM_015474059.1	XP_015329545.1	ral GTPase-activating protein subunit alpha-2 isoform X5
XM_015474064.1	XP_015329550.1	ral GTPase-activating protein subunit alpha-2 isoform X8
XM_015474045.1	XP_015329531.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_015474049.1	XP_015329535.1	ral GTPase-activating protein subunit alpha-2 isoform X3
XM_010822477.2	XP_010820779.2	ral GTPase-activating protein subunit alpha-2 isoform X13
XM_015465968.1	XP_015321454.1	ral GTPase-activating protein subunit alpha-2 isoform X9
XM_015465969.1	XP_015321455.1	ral GTPase-activating protein subunit alpha-2 isoform X10
XM_015465973.1	XP_015321459.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_010822473.2	XP_010820775.2	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_015465976.1	XP_015321462.1	ral GTPase-activating protein subunit alpha-2 isoform X11
XM_015465977.1	XP_015321463.1	ral GTPase-activating protein subunit alpha-2 isoform X12
XM_010822476.2	XP_010820778.2	ral GTPase-activating protein subunit alpha-2 isoform X13
XM_025001041.1	XP_024856809.1	ral GTPase-activating protein subunit alpha-2 isoform X2
XM_025001043.1	XP_024856811.1	ral GTPase-activating protein subunit alpha-2 isoform X4

Pathways from BioSystems

Homologies

	RALGAPA2	XP_001092526.2
Bos taurus (cattle)	RALGAPA2	XP_005214436.1
Rattus norvegicus (Norway rat)	Ralgapa2	XP_006235240.1
Homo sapiens (human)	RALGAPA2	NP_065076.2
Xenopus tropicalis (tropical clawed frog)	ralgapa2	NP_001120298.1
Gallus gallus (chicken)	RALGAPA2	XP_004940197.1
Canis lupus familiaris (dog)	RALGAPA2	XP_005634770.1
Danio rerio (zebrafish)	ralgapa2	XP_005158962.1
Pan troglodytes (chimpanzee)	RALGAPA2	XP_001144701.3
Mus musculus (house mouse)	Ralgapa2	NP_001028520.2

Gene Ontology

The following RALGAPA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221909	XM_025001040.1 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221910	XM_025001041.1 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221911	XM_025001042.1 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221912	XM_025001043.1 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221913	XM_025001044.1 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221914	XM_025001045.1 Latest version!	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa92268	XM_015474043.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92269	XM_015474044.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474052.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474050.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474051.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474049.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474053.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474045.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474047.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474048.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92270	XM_015474046.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92271	XM_015474056.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92271	XM_015474055.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92271	XM_015474054.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92271	XM_015474057.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92272	XM_015474060.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92272	XM_015474058.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92272	XM_015474059.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92272	XM_015474061.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92273	XM_015474062.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92274	XM_015474063.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92275	XM_015474064.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94787	XM_015465968.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94788	XM_015465969.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465972.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_010822472.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465973.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465971.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465970.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_010822473.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465976.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465975.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94789	XM_015465974.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94790	XM_015465979.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94790	XM_015465978.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94790	XM_015465980.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94790	XM_015465977.1 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94791	XM_010822477.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94791	XM_010822474.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94791	XM_010822476.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94791	XM_010822475.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa94792	XM_010822479.2 Latest version!	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RALGAPA2 ( XM_025001040.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856808.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X1, mRNA.

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CloneID	OBa221909
Clone ID Related Accession (Same CDS sequence)	XM_025001040.1
Accession Version	XM_025001040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC 
AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT 
CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT 
GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC 
ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG 
GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT 
ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT 
CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA 
ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC 
ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT 
GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC 
ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA 
GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC 
ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC 
GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC 
ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT 
GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA 
TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC 
AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG 
GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT 
GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT 
GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT 
AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG 
AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT 
AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC 
TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA 
TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG 
ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA 
GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG 
CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC 
GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGCTAAGCGA 
AGCCAGTCTA TCAGCAACTG TGCCCACCTC TCTGAGGCTT TGCCCGCTAC TAAAAGCGTC 
CCACTCCTCC TCCACACCGT GAGCGCTCTC TTCCCTGGTC TCTCTTACTC TTCTTGCTCC 
CACAGGCTGT CAGAAGTGGA AGAATACCTG CAGCCAGAAA ACACACCTGC CACCGAATCT 
GGGCCCCTGG GGCAGCAGCC GATGGTTCGG AGCGGCAGCA GCCCTGAGGT CACCGAGCCG 
CTGTGTCCTG AGCCCCCCCC AGGCCAAAAG CTAGAAAATG CACAGAACGT GAGTTCTTCA 
GAGCCCAGAC TTGCTCAGGA GAGCAAAGGA CATGAGAAGA AAGAGCATGA AGGAATAACA 
ATCTTAGTTC GAAGAAGCAG CAGCCCCATT GAACTGGATT TGAAAGATGA CTCACAGCAG 
ATGCAAGGAA AACGTAGGGA GAGACAGAAG AGTGACAGTG TGAGCAGTGA CCCCACTCTG 
GGCTGCAGCA CTGAGGCAGA GCTGTCCCTG AGCCACTGGC AGACCTGCGA GGAAGACCCA 
GATCTGAGCC CGCCTGCAGA TACTGTGACC GACGCTGACG CCCGCCACTG GTTACAGCTC 
CGGCCCACGG ATGCTTCAAA CCTAACAGAC AGCAGCGAAT GCCTTGCTGA TGACTGCAGT 
ATAATTGCTG GGGGGACCCT CACTGGTTGG CACCCAGACT CAGCCGCTGT GTTGTGGCGA 
AGAATCTTGG GAATCCTTGG AGATGTGAAT AATATCCAGT CACCCAAAAT CCATGCCAGG 
GTGTTTGGCT ATCTCTATGA ACTCTGGTAC AAGCTAGCAA AGATACGGGA TAACCTGGCA 
ATAAGCCTGG ATAACCAGTC TTCTCCATCT CCTCCTGCCT TGATCCCACC GCTCAGAATG 
TTTGCATCAT GGCTATTTAA GGCGACGACG ATGCCTGACG ATTATAAGGA AGGCAAACTG 
CAAGCCTATA AGCTGATCTG CTCCATGATG ACCCGGCGCC AGGACGTCCT GCCCAACTCA 
GACTTCCTGG TGCATTTTTA CCTCGTCATG CACCTGGGAC TGACCAGCGA GGACCAGGAT 
ATCCTGAATA CCATCATAAG GCACTGCCCG CCCCGCTTCT TCTCCCTGGG CTTGCCTGGC 
TTCTCCATGC TGGTGGGGGA CTTCATCATG GCTGCTGGCA GGGTCCTCAG TGCAGACACG 
CTGGCGGCAC CTCGTTCGGA AGCCCTCACC ATTCTCGGGT CTCTGGTCTG CTTTCCGAAC 
ACCTACCGCG AGATTCCCCT ACGGCAGCCG GTACCTGAAG TTGCTGAAGT CGCCATGGGA 
GCAGAAGATG TCAAGCACTG CCTCATAAGT ATCTTACTGA AGAATGCCAC AGAGGAACCG 
AGTGAGTGTG CAAGGTGCAT TGCCATCTGC TCCCTCGGGA TCTGGATCTG TGAGGAGCTG 
GCACAAGGCA CTGGCCACCC TCAGCTGCAA GAGGCCGTCA ACGTCATTGG TGTCACGTTG 
AAGTTTCCTA ACAAAATTGT GGCCCAGGTA GCCTGTGACG TGCTTCAGCT GCTAGTCTCC 
TACTGGGAAA AGCTTCAGAT GTTTGAAACC TCCTTGCCTC GGAAAATTGC AGAAATCCTT 
GTGGCCACAA TTGCTTTTCT GTTACCAAAT GCAGAGTACT CCTCGGTGGA GACAGACAAG 
AAGTTCCTCG TGTCCTTGTT GCTCTGCCTC CTGGACTGGT GCATGGCGCT GCCGGCAGGA 
ACCCTCCTCC ACCCCGTGTC CACGGCGGCC CTGGACGAAC AGGCCCCGGC CCGGGCCCCC 
TTACTGGATT ACATCTACAG GGTTCTGCAC TGCTGCATCT GTGGCTCAAG CACGTACAGC 
CAGCAGAGCC GCTACACCTT GACCCTGGCG GACCTGTCGT CCTCGGATTA CGACCCCTTC 
CTGCCGCTGG CGAACGTGAA GAATTCTGAG CCGGTCCAAT TTCATTCATC AGCAGAACTG 
GGCAACCTGC TCACTGTTGA AGAGGCAGAG AGGAGGAGAA GGAGCCTGGG GCTGATCCCG 
CTGACAGCGC GCATGGTGAT GGCGCACCTG GTCAACCACC TGGGCCACTT CCCGCTCTGC 
GGGGGCCCCG CCGTGCTGCA CAGCCTGGTG GGCGAGCACC ACGACAACGT GCACGCCGAC 
GCCGCCGAGC TCTCCCCAGA CGTGTTCAGG AGCCCCAGCC TGCAGCTGTT TGTGTTCAAC 
GACAGCACCC TCATCTCCTA CCTGCAGACG CCTGCGACCG GTCCTGCCAG TGGGTCGCCA 
GCGGGCGCCC CCTCGGACGT GAGAGTGATC GTGAGGGACA TCTCAGGGAA GTACTCGTGG 
GACGGGAAGG TGCTCTACGG ACCCCTGGAA GGCCACTTAG CCCCCAAGGG CAGAAGGCCC 
TCGTTCCTCA TCTCGAGCTG TCAACCCCCA ACCCCTGGAC CTCAGAGGGC TCCTTCTCAG 
ACTGAGGAGG GAGAGGATGT CCTCGACAAA TTACTTGAAA ACCTTGGCCA CACGAGTCCT 
GAATGCCTTT TGCCATCCCA GCTCCGTCTG AATGAGCCTT CCCCACCCCC TTGTGGCATG 
AATTGGGACC AGGAGAAGGA GATCACCAAG GTCCTGCTGC GCCAGAGCAC GCAAGAAGAC 
GAGTATGTCC AGAGGTGCAA CTCGGATGTG GCCATGAGGG TCGCCAGCCA GGAGCCGCCG 
TCCCCAGTGG AGCCCCGGGG TGCCTTCTAC TTCTGTAGGC TGCTGCTCGA TGACTTGGGA 
ATGAATTCTT GGGACAGAAG GAAGAACTTC CATCTGTTGA AGAAAAATTC AAAATTATTG 
AGAGAGCTAA AAAATCTGGA CTCCCGGCAG TGCCGTGAAA CGCACAAAAT CGCAGTGTTT 
TACATTGCCG AAGGTCAAGA AGACAAGTGT TCGATCCTTT CCAATGAAAG GGGAAGCCAA 
GCGTATGAAG ACTTTGTTGC TGGACTTGGA TGGGAGGTGG ACCTGTCAAC CCACTGTGGG 
TTCATGGGCG GGCTTCAGCG CAATGGCAGC ACGGGCCAGA CCGCCCCCTA CTACGCTACC 
TCGACCGTGG AAGTCATCTT CCACGTCTCA ACTCGGATGC CGTCAGACTC AGACGACTCG 
CTCACCAAAA AGCTCCGTCA CTTGGGGAAT GACGAAGTCC ACATCGTCTG GTCCGAGCAC 
TCCAGGGACT ACCGCAGCGG GATCATCCCC ACCGCCTTCG GAGACGTTTC AATCATCATC 
TACCCCATGA AGAATCACAT GTTCTTTATC GCGATAACCA AGAAACCCGA GGTTCCATTT 
TTTGGGCCTC TGTTTGATGG AGCAATCGTG AGTGGGAAGC TACTGCCAAG CCTTATCTGT 
GCCACCTGCA TCAACGCCAG CCGGGCCGTC AAGTGCCTCA TCCCCCTCTA CCAGAGCTTC 
TACGAGGAGA GAGCTCTCTA TCTGGAAGCA ATAATTCAGA ACCACCGTGA AGTCATGACA 
TTTGAGGATT TCACAGCCCA GGTCTTCTGT CCATCTCCCA GCTACTCGGT CAGCGGAACT 
GTGTCAGCTC TGGAAGAGGG AAGGGATGGA TGTGATGAAC ATCTGCTCCA CCTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856808.1
CDS	117..5936
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIMAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADAAELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRRPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_025001040.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001040.1

ORF Insert Sequence:

ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC 
AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT 
CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT 
GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC 
ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG 
GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT 
ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT 
CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA 
ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC 
ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT 
GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC 
ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA 
GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC 
ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC 
GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC 
ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT 
GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA 
TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC 
AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG 
GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT 
GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT 
GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT 
AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG 
AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT 
AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC 
TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA 
TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG 
ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA 
GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG 
CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC 
GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGCTAAGCGA 
AGCCAGTCTA TCAGCAACTG TGCCCACCTC TCTGAGGCTT TGCCCGCTAC TAAAAGCGTC 
CCACTCCTCC TCCACACCGT GAGCGCTCTC TTCCCTGGTC TCTCTTACTC TTCTTGCTCC 
CACAGGCTGT CAGAAGTGGA AGAATACCTG CAGCCAGAAA ACACACCTGC CACCGAATCT 
GGGCCCCTGG GGCAGCAGCC GATGGTTCGG AGCGGCAGCA GCCCTGAGGT CACCGAGCCG 
CTGTGTCCTG AGCCCCCCCC AGGCCAAAAG CTAGAAAATG CACAGAACGT GAGTTCTTCA 
GAGCCCAGAC TTGCTCAGGA GAGCAAAGGA CATGAGAAGA AAGAGCATGA AGGAATAACA 
ATCTTAGTTC GAAGAAGCAG CAGCCCCATT GAACTGGATT TGAAAGATGA CTCACAGCAG 
ATGCAAGGAA AACGTAGGGA GAGACAGAAG AGTGACAGTG TGAGCAGTGA CCCCACTCTG 
GGCTGCAGCA CTGAGGCAGA GCTGTCCCTG AGCCACTGGC AGACCTGCGA GGAAGACCCA 
GATCTGAGCC CGCCTGCAGA TACTGTGACC GACGCTGACG CCCGCCACTG GTTACAGCTC 
CGGCCCACGG ATGCTTCAAA CCTAACAGAC AGCAGCGAAT GCCTTGCTGA TGACTGCAGT 
ATAATTGCTG GGGGGACCCT CACTGGTTGG CACCCAGACT CAGCCGCTGT GTTGTGGCGA 
AGAATCTTGG GAATCCTTGG AGATGTGAAT AATATCCAGT CACCCAAAAT CCATGCCAGG 
GTGTTTGGCT ATCTCTATGA ACTCTGGTAC AAGCTAGCAA AGATACGGGA TAACCTGGCA 
ATAAGCCTGG ATAACCAGTC TTCTCCATCT CCTCCTGCCT TGATCCCACC GCTCAGAATG 
TTTGCATCAT GGCTATTTAA GGCGACGACG ATGCCTGACG ATTATAAGGA AGGCAAACTG 
CAAGCCTATA AGCTGATCTG CTCCATGATG ACCCGGCGCC AGGACGTCCT GCCCAACTCA 
GACTTCCTGG TGCATTTTTA CCTCGTCATG CACCTGGGAC TGACCAGCGA GGACCAGGAT 
ATCCTGAATA CCATCATAAG GCACTGCCCG CCCCGCTTCT TCTCCCTGGG CTTGCCTGGC 
TTCTCCATGC TGGTGGGGGA CTTCATCATG GCTGCTGGCA GGGTCCTCAG TGCAGACACG 
CTGGCGGCAC CTCGTTCGGA AGCCCTCACC ATTCTCGGGT CTCTGGTCTG CTTTCCGAAC 
ACCTACCGCG AGATTCCCCT ACGGCAGCCG GTACCTGAAG TTGCTGAAGT CGCCATGGGA 
GCAGAAGATG TCAAGCACTG CCTCATAAGT ATCTTACTGA AGAATGCCAC AGAGGAACCG 
AGTGAGTGTG CAAGGTGCAT TGCCATCTGC TCCCTCGGGA TCTGGATCTG TGAGGAGCTG 
GCACAAGGCA CTGGCCACCC TCAGCTGCAA GAGGCCGTCA ACGTCATTGG TGTCACGTTG 
AAGTTTCCTA ACAAAATTGT GGCCCAGGTA GCCTGTGACG TGCTTCAGCT GCTAGTCTCC 
TACTGGGAAA AGCTTCAGAT GTTTGAAACC TCCTTGCCTC GGAAAATTGC AGAAATCCTT 
GTGGCCACAA TTGCTTTTCT GTTACCAAAT GCAGAGTACT CCTCGGTGGA GACAGACAAG 
AAGTTCCTCG TGTCCTTGTT GCTCTGCCTC CTGGACTGGT GCATGGCGCT GCCGGCAGGA 
ACCCTCCTCC ACCCCGTGTC CACGGCGGCC CTGGACGAAC AGGCCCCGGC CCGGGCCCCC 
TTACTGGATT ACATCTACAG GGTTCTGCAC TGCTGCATCT GTGGCTCAAG CACGTACAGC 
CAGCAGAGCC GCTACACCTT GACCCTGGCG GACCTGTCGT CCTCGGATTA CGACCCCTTC 
CTGCCGCTGG CGAACGTGAA GAATTCTGAG CCGGTCCAAT TTCATTCATC AGCAGAACTG 
GGCAACCTGC TCACTGTTGA AGAGGCAGAG AGGAGGAGAA GGAGCCTGGG GCTGATCCCG 
CTGACAGCGC GCATGGTGAT GGCGCACCTG GTCAACCACC TGGGCCACTT CCCGCTCTGC 
GGGGGCCCCG CCGTGCTGCA CAGCCTGGTG GGCGAGCACC ACGACAACGT GCACGCCGAC 
GCCGCCGAGC TCTCCCCAGA CGTGTTCAGG AGCCCCAGCC TGCAGCTGTT TGTGTTCAAC 
GACAGCACCC TCATCTCCTA CCTGCAGACG CCTGCGACCG GTCCTGCCAG TGGGTCGCCA 
GCGGGCGCCC CCTCGGACGT GAGAGTGATC GTGAGGGACA TCTCAGGGAA GTACTCGTGG 
GACGGGAAGG TGCTCTACGG ACCCCTGGAA GGCCACTTAG CCCCCAAGGG CAGAAGGCCC 
TCGTTCCTCA TCTCGAGCTG TCAACCCCCA ACCCCTGGAC CTCAGAGGGC TCCTTCTCAG 
ACTGAGGAGG GAGAGGATGT CCTCGACAAA TTACTTGAAA ACCTTGGCCA CACGAGTCCT 
GAATGCCTTT TGCCATCCCA GCTCCGTCTG AATGAGCCTT CCCCACCCCC TTGTGGCATG 
AATTGGGACC AGGAGAAGGA GATCACCAAG GTCCTGCTGC GCCAGAGCAC GCAAGAAGAC 
GAGTATGTCC AGAGGTGCAA CTCGGATGTG GCCATGAGGG TCGCCAGCCA GGAGCCGCCG 
TCCCCAGTGG AGCCCCGGGG TGCCTTCTAC TTCTGTAGGC TGCTGCTCGA TGACTTGGGA 
ATGAATTCTT GGGACAGAAG GAAGAACTTC CATCTGTTGA AGAAAAATTC AAAATTATTG 
AGAGAGCTAA AAAATCTGGA CTCCCGGCAG TGCCGTGAAA CGCACAAAAT CGCAGTGTTT 
TACATTGCCG AAGGTCAAGA AGACAAGTGT TCGATCCTTT CCAATGAAAG GGGAAGCCAA 
GCGTATGAAG ACTTTGTTGC TGGACTTGGA TGGGAGGTGG ACCTGTCAAC CCACTGTGGG 
TTCATGGGCG GGCTTCAGCG CAATGGCAGC ACGGGCCAGA CCGCCCCCTA CTACGCTACC 
TCGACCGTGG AAGTCATCTT CCACGTCTCA ACTCGGATGC CGTCAGACTC AGACGACTCG 
CTCACCAAAA AGCTCCGTCA CTTGGGGAAT GACGAAGTCC ACATCGTCTG GTCCGAGCAC 
TCCAGGGACT ACCGCAGCGG GATCATCCCC ACCGCCTTCG GAGACGTTTC AATCATCATC 
TACCCCATGA AGAATCACAT GTTCTTTATC GCGATAACCA AGAAACCCGA GGTTCCATTT 
TTTGGGCCTC TGTTTGATGG AGCAATCGTG AGTGGGAAGC TACTGCCAAG CCTTATCTGT 
GCCACCTGCA TCAACGCCAG CCGGGCCGTC AAGTGCCTCA TCCCCCTCTA CCAGAGCTTC 
TACGAGGAGA GAGCTCTCTA TCTGGAAGCA ATAATTCAGA ACCACCGTGA AGTCATGACA 
TTTGAGGATT TCACAGCCCA GGTCTTCTGT CCATCTCCCA GCTACTCGGT CAGCGGAACT 
GTGTCAGCTC TGGAAGAGGG AAGGGATGGA TGTGATGAAC ATCTGCTCCA CCTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_025001041.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856809.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X2, mRNA.

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CloneID	OBa221910
Clone ID Related Accession (Same CDS sequence)	XM_025001041.1
Accession Version	XM_025001041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA 
TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA 
TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT 
AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG 
TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG 
AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT 
CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC 
CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC 
ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG 
CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG 
CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG 
GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT 
CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA 
CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC 
CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA 
AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT 
CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG 
GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC 
GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC 
ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT 
TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG 
ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA 
GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC 
AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA 
GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG 
GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG 
CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA 
ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA 
ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC 
TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG 
TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG 
GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG 
ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA 
AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG 
TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC 
AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG 
AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG 
CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG 
TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG 
CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC 
TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG 
CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC 
TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT 
CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG 
CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA 
ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA 
ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG 
TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA 
AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT 
GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA 
GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC 
TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC 
CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC 
TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG 
GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC 
TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA 
GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT 
GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA 
CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG 
TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC 
TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG 
GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG 
TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC 
CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA 
CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG 
CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG 
CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC 
AACCTGCTCA CTGTTGAAGA GGCAGAGAGG AGGAGAAGGA GCCTGGGGCT GATCCCGCTG 
ACAGCGCGCA TGGTGATGGC GCACCTGGTC AACCACCTGG GCCACTTCCC GCTCTGCGGG 
GGCCCCGCCG TGCTGCACAG CCTGGTGGGC GAGCACCACG ACAACGTGCA CGCCGACGCC 
GCCGAGCTCT CCCCAGACGT GTTCAGGAGC CCCAGCCTGC AGCTGTTTGT GTTCAACGAC 
AGCACCCTCA TCTCCTACCT GCAGACGCCT GCGACCGGTC CTGCCAGTGG GTCGCCAGCG 
GGCGCCCCCT CGGACGTGAG AGTGATCGTG AGGGACATCT CAGGGAAGTA CTCGTGGGAC 
GGGAAGGTGC TCTACGGACC CCTGGAAGGC CACTTAGCCC CCAAGGGCAG AAGGCCCTCG 
TTCCTCATCT CGAGCTGTCA ACCCCCAACC CCTGGACCTC AGAGGGCTCC TTCTCAGACT 
GAGGAGGGAG AGGATGTCCT CGACAAATTA CTTGAAAACC TTGGCCACAC GAGTCCTGAA 
TGCCTTTTGC CATCCCAGCT CCGTCTGAAT GAGCCTTCCC CACCCCCTTG TGGCATGAAT 
TGGGACCAGG AGAAGGAGAT CACCAAGGTC CTGCTGCGCC AGAGCACGCA AGAAGACGAG 
TATGTCCAGA GGTGCAACTC GGATGTGGCC ATGAGGGTCG CCAGCCAGGA GCCGCCGTCC 
CCAGTGGAGC CCCGGGGTGC CTTCTACTTC TGTAGGCTGC TGCTCGATGA CTTGGGAATG 
AATTCTTGGG ACAGAAGGAA GAACTTCCAT CTGTTGAAGA AAAATTCAAA ATTATTGAGA 
GAGCTAAAAA ATCTGGACTC CCGGCAGTGC CGTGAAACGC ACAAAATCGC AGTGTTTTAC 
ATTGCCGAAG GTCAAGAAGA CAAGTGTTCG ATCCTTTCCA ATGAAAGGGG AAGCCAAGCG 
TATGAAGACT TTGTTGCTGG ACTTGGATGG GAGGTGGACC TGTCAACCCA CTGTGGGTTC 
ATGGGCGGGC TTCAGCGCAA TGGCAGCACG GGCCAGACCG CCCCCTACTA CGCTACCTCG 
ACCGTGGAAG TCATCTTCCA CGTCTCAACT CGGATGCCGT CAGACTCAGA CGACTCGCTC 
ACCAAAAAGC TCCGTCACTT GGGGAATGAC GAAGTCCACA TCGTCTGGTC CGAGCACTCC 
AGGGACTACC GCAGCGGGAT CATCCCCACC GCCTTCGGAG ACGTTTCAAT CATCATCTAC 
CCCATGAAGA ATCACATGTT CTTTATCGCG ATAACCAAGA AACCCGAGGT TCCATTTTTT 
GGGCCTCTGT TTGATGGAGC AATCGTGAGT GGGAAGCTAC TGCCAAGCCT TATCTGTGCC 
ACCTGCATCA ACGCCAGCCG GGCCGTCAAG TGCCTCATCC CCCTCTACCA GAGCTTCTAC 
GAGGAGAGAG CTCTCTATCT GGAAGCAATA ATTCAGAACC ACCGTGAAGT CATGACATTT 
GAGGATTTCA CAGCCCAGGT CTTCTGTCCA TCTCCCAGCT ACTCGGTCAG CGGAACTGTG 
TCAGCTCTGG AAGAGGGAAG GGATGGATGT GATGAACATC TGCTCCACCT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856809.1
CDS	43..5739
Translation	MDQSQGTPVLPTKATLDQPVASGLPDMEAVSAKMSQVQPRSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIMAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADAAELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRRPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_025001041.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001041.1

ORF Insert Sequence:

ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA 
TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA 
TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT 
AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG 
TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG 
AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT 
CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC 
CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC 
ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG 
CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG 
CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG 
GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT 
CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA 
CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC 
CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA 
AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT 
CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG 
GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC 
GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC 
ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT 
TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG 
ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA 
GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC 
AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA 
GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG 
GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG 
CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA 
ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA 
ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC 
TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG 
TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG 
GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG 
ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA 
AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG 
TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC 
AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG 
AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG 
CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG 
TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG 
CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC 
TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG 
CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC 
TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT 
CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG 
CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA 
ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA 
ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG 
TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA 
AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT 
GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA 
GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC 
TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC 
CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC 
TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG 
GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC 
TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA 
GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT 
GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA 
CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG 
TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC 
TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG 
GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG 
TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC 
CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA 
CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG 
CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG 
CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC 
AACCTGCTCA CTGTTGAAGA GGCAGAGAGG AGGAGAAGGA GCCTGGGGCT GATCCCGCTG 
ACAGCGCGCA TGGTGATGGC GCACCTGGTC AACCACCTGG GCCACTTCCC GCTCTGCGGG 
GGCCCCGCCG TGCTGCACAG CCTGGTGGGC GAGCACCACG ACAACGTGCA CGCCGACGCC 
GCCGAGCTCT CCCCAGACGT GTTCAGGAGC CCCAGCCTGC AGCTGTTTGT GTTCAACGAC 
AGCACCCTCA TCTCCTACCT GCAGACGCCT GCGACCGGTC CTGCCAGTGG GTCGCCAGCG 
GGCGCCCCCT CGGACGTGAG AGTGATCGTG AGGGACATCT CAGGGAAGTA CTCGTGGGAC 
GGGAAGGTGC TCTACGGACC CCTGGAAGGC CACTTAGCCC CCAAGGGCAG AAGGCCCTCG 
TTCCTCATCT CGAGCTGTCA ACCCCCAACC CCTGGACCTC AGAGGGCTCC TTCTCAGACT 
GAGGAGGGAG AGGATGTCCT CGACAAATTA CTTGAAAACC TTGGCCACAC GAGTCCTGAA 
TGCCTTTTGC CATCCCAGCT CCGTCTGAAT GAGCCTTCCC CACCCCCTTG TGGCATGAAT 
TGGGACCAGG AGAAGGAGAT CACCAAGGTC CTGCTGCGCC AGAGCACGCA AGAAGACGAG 
TATGTCCAGA GGTGCAACTC GGATGTGGCC ATGAGGGTCG CCAGCCAGGA GCCGCCGTCC 
CCAGTGGAGC CCCGGGGTGC CTTCTACTTC TGTAGGCTGC TGCTCGATGA CTTGGGAATG 
AATTCTTGGG ACAGAAGGAA GAACTTCCAT CTGTTGAAGA AAAATTCAAA ATTATTGAGA 
GAGCTAAAAA ATCTGGACTC CCGGCAGTGC CGTGAAACGC ACAAAATCGC AGTGTTTTAC 
ATTGCCGAAG GTCAAGAAGA CAAGTGTTCG ATCCTTTCCA ATGAAAGGGG AAGCCAAGCG 
TATGAAGACT TTGTTGCTGG ACTTGGATGG GAGGTGGACC TGTCAACCCA CTGTGGGTTC 
ATGGGCGGGC TTCAGCGCAA TGGCAGCACG GGCCAGACCG CCCCCTACTA CGCTACCTCG 
ACCGTGGAAG TCATCTTCCA CGTCTCAACT CGGATGCCGT CAGACTCAGA CGACTCGCTC 
ACCAAAAAGC TCCGTCACTT GGGGAATGAC GAAGTCCACA TCGTCTGGTC CGAGCACTCC 
AGGGACTACC GCAGCGGGAT CATCCCCACC GCCTTCGGAG ACGTTTCAAT CATCATCTAC 
CCCATGAAGA ATCACATGTT CTTTATCGCG ATAACCAAGA AACCCGAGGT TCCATTTTTT 
GGGCCTCTGT TTGATGGAGC AATCGTGAGT GGGAAGCTAC TGCCAAGCCT TATCTGTGCC 
ACCTGCATCA ACGCCAGCCG GGCCGTCAAG TGCCTCATCC CCCTCTACCA GAGCTTCTAC 
GAGGAGAGAG CTCTCTATCT GGAAGCAATA ATTCAGAACC ACCGTGAAGT CATGACATTT 
GAGGATTTCA CAGCCCAGGT CTTCTGTCCA TCTCCCAGCT ACTCGGTCAG CGGAACTGTG 
TCAGCTCTGG AAGAGGGAAG GGATGGATGT GATGAACATC TGCTCCACCT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_025001042.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856810.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X3, mRNA.

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CloneID	OBa221911
Clone ID Related Accession (Same CDS sequence)	XM_025001042.1
Accession Version	XM_025001042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA 
TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA 
TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT 
AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG 
TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG 
AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT 
CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC 
CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC 
ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG 
CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG 
CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG 
GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT 
CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA 
CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC 
CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA 
AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT 
CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG 
GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC 
GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC 
ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT 
TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG 
ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA 
GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC 
AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA 
GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG 
GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG 
CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA 
ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA 
ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC 
TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG 
TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG 
GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG 
ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA 
AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG 
TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC 
AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG 
AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG 
CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG 
TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG 
CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC 
TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG 
CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC 
TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT 
CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG 
CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA 
ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA 
ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG 
TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA 
AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT 
GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA 
GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC 
TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC 
CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC 
TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG 
GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC 
TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA 
GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT 
GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA 
CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG 
TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC 
TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG 
GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG 
TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC 
CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA 
CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG 
CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG 
CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC 
AACCTGCTCA CTGTTGAAGA GGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCGCC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG GCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGTGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856810.1
CDS	43..5721
Translation	MDQSQGTPVLPTKATLDQPVASGLPDMEAVSAKMSQVQPRSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIMAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADAAELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRRPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_025001042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001042.1

ORF Insert Sequence:

ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA 
TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA 
TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT 
AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG 
TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG 
AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT 
CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC 
CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC 
ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG 
CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG 
CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG 
GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT 
CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA 
CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC 
CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA 
AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT 
CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG 
GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC 
GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC 
ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT 
TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG 
ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA 
GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC 
AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA 
GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG 
GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG 
CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA 
ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA 
ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC 
TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG 
TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG 
GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG 
ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA 
AAGAAACAGC GAGGCAAAGG CTGTGTTCCA GATTCTCAGA AAGGAAGCAC CGTGGGGAGG 
TCCTTTTCTC TGAGCTGGCG GAGCCACCCC GACGTGGCCG TGGCCGAGCC CATGAGATTC 
AGGAGCGCCA CCACGTCCGG GGCGCCAGGG GTCGAGAAAG CCAGAAACGT TGTCCGCCAG 
AAGGCAACCG AAGTGGAAGA ATACCTGCAG CCAGAAAACA CACCTGCCAC CGAATCTGGG 
CCCCTGGGGC AGCAGCCGAT GGTTCGGAGC GGCAGCAGCC CTGAGGTCAC CGAGCCGCTG 
TGTCCTGAGC CCCCCCCAGG CCAAAAGCTA GAAAATGCAC AGAACGTGAG TTCTTCAGAG 
CCCAGACTTG CTCAGGAGAG CAAAGGACAT GAGAAGAAAG AGCATGAAGG AATAACAATC 
TTAGTTCGAA GAAGCAGCAG CCCCATTGAA CTGGATTTGA AAGATGACTC ACAGCAGATG 
CAAGGAAAAC GTAGGGAGAG ACAGAAGAGT GACAGTGTGA GCAGTGACCC CACTCTGGGC 
TGCAGCACTG AGGCAGAGCT GTCCCTGAGC CACTGGCAGA CCTGCGAGGA AGACCCAGAT 
CTGAGCCCGC CTGCAGATAC TGTGACCGAC GCTGACGCCC GCCACTGGTT ACAGCTCCGG 
CCCACGGATG CTTCAAACCT AACAGACAGC AGCGAATGCC TTGCTGATGA CTGCAGTATA 
ATTGCTGGGG GGACCCTCAC TGGTTGGCAC CCAGACTCAG CCGCTGTGTT GTGGCGAAGA 
ATCTTGGGAA TCCTTGGAGA TGTGAATAAT ATCCAGTCAC CCAAAATCCA TGCCAGGGTG 
TTTGGCTATC TCTATGAACT CTGGTACAAG CTAGCAAAGA TACGGGATAA CCTGGCAATA 
AGCCTGGATA ACCAGTCTTC TCCATCTCCT CCTGCCTTGA TCCCACCGCT CAGAATGTTT 
GCATCATGGC TATTTAAGGC GACGACGATG CCTGACGATT ATAAGGAAGG CAAACTGCAA 
GCCTATAAGC TGATCTGCTC CATGATGACC CGGCGCCAGG ACGTCCTGCC CAACTCAGAC 
TTCCTGGTGC ATTTTTACCT CGTCATGCAC CTGGGACTGA CCAGCGAGGA CCAGGATATC 
CTGAATACCA TCATAAGGCA CTGCCCGCCC CGCTTCTTCT CCCTGGGCTT GCCTGGCTTC 
TCCATGCTGG TGGGGGACTT CATCATGGCT GCTGGCAGGG TCCTCAGTGC AGACACGCTG 
GCGGCACCTC GTTCGGAAGC CCTCACCATT CTCGGGTCTC TGGTCTGCTT TCCGAACACC 
TACCGCGAGA TTCCCCTACG GCAGCCGGTA CCTGAAGTTG CTGAAGTCGC CATGGGAGCA 
GAAGATGTCA AGCACTGCCT CATAAGTATC TTACTGAAGA ATGCCACAGA GGAACCGAGT 
GAGTGTGCAA GGTGCATTGC CATCTGCTCC CTCGGGATCT GGATCTGTGA GGAGCTGGCA 
CAAGGCACTG GCCACCCTCA GCTGCAAGAG GCCGTCAACG TCATTGGTGT CACGTTGAAG 
TTTCCTAACA AAATTGTGGC CCAGGTAGCC TGTGACGTGC TTCAGCTGCT AGTCTCCTAC 
TGGGAAAAGC TTCAGATGTT TGAAACCTCC TTGCCTCGGA AAATTGCAGA AATCCTTGTG 
GCCACAATTG CTTTTCTGTT ACCAAATGCA GAGTACTCCT CGGTGGAGAC AGACAAGAAG 
TTCCTCGTGT CCTTGTTGCT CTGCCTCCTG GACTGGTGCA TGGCGCTGCC GGCAGGAACC 
CTCCTCCACC CCGTGTCCAC GGCGGCCCTG GACGAACAGG CCCCGGCCCG GGCCCCCTTA 
CTGGATTACA TCTACAGGGT TCTGCACTGC TGCATCTGTG GCTCAAGCAC GTACAGCCAG 
CAGAGCCGCT ACACCTTGAC CCTGGCGGAC CTGTCGTCCT CGGATTACGA CCCCTTCCTG 
CCGCTGGCGA ACGTGAAGAA TTCTGAGCCG GTCCAATTTC ATTCATCAGC AGAACTGGGC 
AACCTGCTCA CTGTTGAAGA GGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCGCC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG GCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGTGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_025001043.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856811.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X4, mRNA.

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CloneID	OBa221912
Clone ID Related Accession (Same CDS sequence)	XM_025001043.1
Accession Version	XM_025001043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC 
AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT 
CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT 
GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC 
ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG 
GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT 
ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT 
CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA 
ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC 
ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT 
GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC 
ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA 
GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC 
ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC 
GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC 
ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT 
GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA 
TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC 
AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG 
GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT 
GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT 
GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT 
AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG 
AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT 
AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC 
TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA 
TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG 
ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA 
GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG 
CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC 
GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA 
GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG 
ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA 
GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG 
AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC 
AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG 
AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG 
CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT 
ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC 
CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC 
ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA 
GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA 
CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT 
TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG 
GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC 
TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC 
CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG 
CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC 
TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA 
GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA 
CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC 
CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT 
GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT 
CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG 
GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG 
TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG 
TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG 
CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC 
ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG 
GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG 
ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG 
AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA 
GAGGCAGAGA GGAGGAGAAG GAGCCTGGGG CTGATCCCGC TGACAGCGCG CATGGTGATG 
GCGCACCTGG TCAACCACCT GGGCCACTTC CCGCTCTGCG GGGGCCCCGC CGTGCTGCAC 
AGCCTGGTGG GCGAGCACCA CGACAACGTG CACGCCGACG CCGCCGAGCT CTCCCCAGAC 
GTGTTCAGGA GCCCCAGCCT GCAGCTGTTT GTGTTCAACG ACAGCACCCT CATCTCCTAC 
CTGCAGACGC CTGCGACCGG TCCTGCCAGT GGGTCGCCAG CGGGCGCCCC CTCGGACGTG 
AGAGTGATCG TGAGGGACAT CTCAGGGAAG TACTCGTGGG ACGGGAAGGT GCTCTACGGA 
CCCCTGGAAG GCCACTTAGC CCCCAAGGGC AGAAGGCCCT CGTTCCTCAT CTCGAGCTGT 
CAACCCCCAA CCCCTGGACC TCAGAGGGCT CCTTCTCAGA CTGAGGAGGG AGAGGATGTC 
CTCGACAAAT TACTTGAAAA CCTTGGCCAC ACGAGTCCTG AATGCCTTTT GCCATCCCAG 
CTCCGTCTGA ATGAGCCTTC CCCACCCCCT TGTGGCATGA ATTGGGACCA GGAGAAGGAG 
ATCACCAAGG TCCTGCTGCG CCAGAGCACG CAAGAAGACG AGTATGTCCA GAGGTGCAAC 
TCGGATGTGG CCATGAGGGT CGCCAGCCAG GAGCCGCCGT CCCCAGTGGA GCCCCGGGGT 
GCCTTCTACT TCTGTAGGCT GCTGCTCGAT GACTTGGGAA TGAATTCTTG GGACAGAAGG 
AAGAACTTCC ATCTGTTGAA GAAAAATTCA AAATTATTGA GAGAGCTAAA AAATCTGGAC 
TCCCGGCAGT GCCGTGAAAC GCACAAAATC GCAGTGTTTT ACATTGCCGA AGGTCAAGAA 
GACAAGTGTT CGATCCTTTC CAATGAAAGG GGAAGCCAAG CGTATGAAGA CTTTGTTGCT 
GGACTTGGAT GGGAGGTGGA CCTGTCAACC CACTGTGGGT TCATGGGCGG GCTTCAGCGC 
AATGGCAGCA CGGGCCAGAC CGCCCCCTAC TACGCTACCT CGACCGTGGA AGTCATCTTC 
CACGTCTCAA CTCGGATGCC GTCAGACTCA GACGACTCGC TCACCAAAAA GCTCCGTCAC 
TTGGGGAATG ACGAAGTCCA CATCGTCTGG TCCGAGCACT CCAGGGACTA CCGCAGCGGG 
ATCATCCCCA CCGCCTTCGG AGACGTTTCA ATCATCATCT ACCCCATGAA GAATCACATG 
TTCTTTATCG CGATAACCAA GAAACCCGAG GTTCCATTTT TTGGGCCTCT GTTTGATGGA 
GCAATCGTGA GTGGGAAGCT ACTGCCAAGC CTTATCTGTG CCACCTGCAT CAACGCCAGC 
CGGGCCGTCA AGTGCCTCAT CCCCCTCTAC CAGAGCTTCT ACGAGGAGAG AGCTCTCTAT 
CTGGAAGCAA TAATTCAGAA CCACCGTGAA GTCATGACAT TTGAGGATTT CACAGCCCAG 
GTCTTCTGTC CATCTCCCAG CTACTCGGTC AGCGGAACTG TGTCAGCTCT GGAAGAGGGA 
AGGGATGGAT GTGATGAACA TCTGCTCCAC CTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856811.1
CDS	116..5794
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIMAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADAAELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRRPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_025001043.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001043.1

ORF Insert Sequence:

ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC 
AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT 
CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT 
GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC 
ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG 
GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT 
ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT 
CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA 
ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC 
ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT 
GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC 
ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA 
GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC 
ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC 
GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC 
ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT 
GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA 
TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC 
AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG 
GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT 
GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT 
GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT 
AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG 
AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT 
AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC 
TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA 
TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG 
ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA 
GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG 
CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC 
GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA 
GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG 
ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA 
GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG 
AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC 
AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG 
AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG 
CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT 
ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC 
CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC 
ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA 
GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA 
CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT 
TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG 
GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC 
TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC 
CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG 
CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC 
TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA 
GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA 
CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC 
CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT 
GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT 
CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG 
GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG 
TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG 
TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG 
CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC 
ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG 
GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG 
ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG 
AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA 
GAGGCAGAGA GGAGGAGAAG GAGCCTGGGG CTGATCCCGC TGACAGCGCG CATGGTGATG 
GCGCACCTGG TCAACCACCT GGGCCACTTC CCGCTCTGCG GGGGCCCCGC CGTGCTGCAC 
AGCCTGGTGG GCGAGCACCA CGACAACGTG CACGCCGACG CCGCCGAGCT CTCCCCAGAC 
GTGTTCAGGA GCCCCAGCCT GCAGCTGTTT GTGTTCAACG ACAGCACCCT CATCTCCTAC 
CTGCAGACGC CTGCGACCGG TCCTGCCAGT GGGTCGCCAG CGGGCGCCCC CTCGGACGTG 
AGAGTGATCG TGAGGGACAT CTCAGGGAAG TACTCGTGGG ACGGGAAGGT GCTCTACGGA 
CCCCTGGAAG GCCACTTAGC CCCCAAGGGC AGAAGGCCCT CGTTCCTCAT CTCGAGCTGT 
CAACCCCCAA CCCCTGGACC TCAGAGGGCT CCTTCTCAGA CTGAGGAGGG AGAGGATGTC 
CTCGACAAAT TACTTGAAAA CCTTGGCCAC ACGAGTCCTG AATGCCTTTT GCCATCCCAG 
CTCCGTCTGA ATGAGCCTTC CCCACCCCCT TGTGGCATGA ATTGGGACCA GGAGAAGGAG 
ATCACCAAGG TCCTGCTGCG CCAGAGCACG CAAGAAGACG AGTATGTCCA GAGGTGCAAC 
TCGGATGTGG CCATGAGGGT CGCCAGCCAG GAGCCGCCGT CCCCAGTGGA GCCCCGGGGT 
GCCTTCTACT TCTGTAGGCT GCTGCTCGAT GACTTGGGAA TGAATTCTTG GGACAGAAGG 
AAGAACTTCC ATCTGTTGAA GAAAAATTCA AAATTATTGA GAGAGCTAAA AAATCTGGAC 
TCCCGGCAGT GCCGTGAAAC GCACAAAATC GCAGTGTTTT ACATTGCCGA AGGTCAAGAA 
GACAAGTGTT CGATCCTTTC CAATGAAAGG GGAAGCCAAG CGTATGAAGA CTTTGTTGCT 
GGACTTGGAT GGGAGGTGGA CCTGTCAACC CACTGTGGGT TCATGGGCGG GCTTCAGCGC 
AATGGCAGCA CGGGCCAGAC CGCCCCCTAC TACGCTACCT CGACCGTGGA AGTCATCTTC 
CACGTCTCAA CTCGGATGCC GTCAGACTCA GACGACTCGC TCACCAAAAA GCTCCGTCAC 
TTGGGGAATG ACGAAGTCCA CATCGTCTGG TCCGAGCACT CCAGGGACTA CCGCAGCGGG 
ATCATCCCCA CCGCCTTCGG AGACGTTTCA ATCATCATCT ACCCCATGAA GAATCACATG 
TTCTTTATCG CGATAACCAA GAAACCCGAG GTTCCATTTT TTGGGCCTCT GTTTGATGGA 
GCAATCGTGA GTGGGAAGCT ACTGCCAAGC CTTATCTGTG CCACCTGCAT CAACGCCAGC 
CGGGCCGTCA AGTGCCTCAT CCCCCTCTAC CAGAGCTTCT ACGAGGAGAG AGCTCTCTAT 
CTGGAAGCAA TAATTCAGAA CCACCGTGAA GTCATGACAT TTGAGGATTT CACAGCCCAG 
GTCTTCTGTC CATCTCCCAG CTACTCGGTC AGCGGAACTG TGTCAGCTCT GGAAGAGGGA 
AGGGATGGAT GTGATGAACA TCTGCTCCAC CTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_025001044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856812.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X5, mRNA.

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CloneID	OBa221913
Clone ID Related Accession (Same CDS sequence)	XM_025001044.1
Accession Version	XM_025001044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC 
AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT 
CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT 
GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC 
ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG 
GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT 
ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT 
CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA 
ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC 
ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT 
GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC 
ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA 
GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC 
ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC 
GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC 
ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT 
GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA 
TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC 
AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG 
GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT 
GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT 
GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT 
AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG 
AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT 
AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC 
TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA 
TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG 
ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA 
GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG 
CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC 
GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA 
GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG 
ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA 
GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG 
AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC 
AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG 
AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG 
CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT 
ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC 
CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC 
ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA 
GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA 
CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT 
TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG 
GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC 
TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC 
CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG 
CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC 
TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA 
GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA 
CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC 
CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT 
GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT 
CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG 
GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG 
TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG 
TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG 
CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC 
ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG 
GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG 
ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG 
AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA 
GAGGAGAGGA GGAGAAGGAG CCTGGGGCTG ATCCCGCTGA CAGCGCGCAT GGTGATGGCG 
CACCTGGTCA ACCACCTGGG CCACTTCCCG CTCTGCGGGG GCCCCGCCGT GCTGCACAGC 
CTGGTGGGCG AGCACCACGA CAACGTGCAC GCCGACGCCG CCGAGCTCTC CCCAGACGTG 
TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG TTCAACGACA GCACCCTCAT CTCCTACCTG 
CAGACGCCTG CGACCGGTCC TGCCAGTGGG TCGCCAGCGG GCGCCCCCTC GGACGTGAGA 
GTGATCGTGA GGGACATCTC AGGGAAGTAC TCGTGGGACG GGAAGGTGCT CTACGGACCC 
CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA AGGCCCTCGT TCCTCATCTC GAGCTGTCAA 
CCCCCAACCC CTGGACCTCA GAGGGCTCCT TCTCAGACTG AGGAGGGAGA GGATGTCCTC 
GACAAATTAC TTGAAAACCT TGGCCACACG AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC 
CGTCTGAATG AGCCTTCCCC ACCCCCTTGT GGCATGAATT GGGACCAGGA GAAGGAGATC 
ACCAAGGTCC TGCTGCGCCA GAGCACGCAA GAAGACGAGT ATGTCCAGAG GTGCAACTCG 
GATGTGGCCA TGAGGGTCGC CAGCCAGGAG CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC 
TTCTACTTCT GTAGGCTGCT GCTCGATGAC TTGGGAATGA ATTCTTGGGA CAGAAGGAAG 
AACTTCCATC TGTTGAAGAA AAATTCAAAA TTATTGAGAG AGCTAAAAAA TCTGGACTCC 
CGGCAGTGCC GTGAAACGCA CAAAATCGCA GTGTTTTACA TTGCCGAAGG TCAAGAAGAC 
AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA AGCCAAGCGT ATGAAGACTT TGTTGCTGGA 
CTTGGATGGG AGGTGGACCT GTCAACCCAC TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT 
GGCAGCACGG GCCAGACCGC CCCCTACTAC GCTACCTCGA CCGTGGAAGT CATCTTCCAC 
GTCTCAACTC GGATGCCGTC AGACTCAGAC GACTCGCTCA CCAAAAAGCT CCGTCACTTG 
GGGAATGACG AAGTCCACAT CGTCTGGTCC GAGCACTCCA GGGACTACCG CAGCGGGATC 
ATCCCCACCG CCTTCGGAGA CGTTTCAATC ATCATCTACC CCATGAAGAA TCACATGTTC 
TTTATCGCGA TAACCAAGAA ACCCGAGGTT CCATTTTTTG GGCCTCTGTT TGATGGAGCA 
ATCGTGAGTG GGAAGCTACT GCCAAGCCTT ATCTGTGCCA CCTGCATCAA CGCCAGCCGG 
GCCGTCAAGT GCCTCATCCC CCTCTACCAG AGCTTCTACG AGGAGAGAGC TCTCTATCTG 
GAAGCAATAA TTCAGAACCA CCGTGAAGTC ATGACATTTG AGGATTTCAC AGCCCAGGTC 
TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC GGAACTGTGT CAGCTCTGGA AGAGGGAAGG 
GATGGATGTG ATGAACATCT GCTCCACCTG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856812.1
CDS	1..5676
Translation	MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIMAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADAAELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRRPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_025001044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001044.1

ORF Insert Sequence:

ATGTTCTCCC GGAGGAGCCA CGGGGATGTG AAGAAGTCCA CCCAGAAGGT GCTGGACCCC 
AAGAAGGACG TGCTGACCCG CCTGAAGCAC CTGCGGGCGC TGCTGGACAA TGTGGATGCC 
AGTGATCTTA AGCAGTTTTT TGAGACCAAC TATTCTCAGA TATATTTCAT CTTCTATGAA 
AATTTTATAA CACTGGAAAA TAGTTTGAAA TTAAAAGGGA ATAATAAGTC ACAAAGGGAG 
GAGCTGGACT CCATTCTCTT TCTTTTTGAA AAAATATTGC AGTTTCTACC TGAACGAATT 
TTCTTTCGAT GGCATTACCA GAGTATAGGC TCAACTTTAA AGAAGCTTCT ACACACTGGA 
AATTCTATTA AGATAAGATG TGAAGGAATC CGGCTGTTTC TTCTGTGGCT TCAAGCACTT 
CAGACGAACT GTGCAGAAGA GCAGGTGCTG ATTTTTGCTA GCCTGGTGCC AGGATTCCCT 
GCCATCATGT CGTCCAGGGG GCCCTGTACA CTGGACACGC TCATCAACCC TGGCCCCAAC 
ATGGCTGATG CGAAGATCCA TCCGGAAGAA ATCACTCCAC TGCTGCCAGC CGTATCAGGG 
GAGAAGATCG CCGAGGACCA AACGTGCTTT TTTCTTCAAG TGCTGTTGAA ATACATGGTT 
ACTCAGGCCG CAAGCTTGGA GTGGAAGCAT AAGGAGAATC AGGAGACTGG GTTTAAATTT 
CTGTTTACGT TGTTTCGAAA GTATTACCTT CCTCATCTGT TTCCCTCGTT TACCAAGTCA 
ACCAACATCT ACAAGCCTGT GCTGGACATC CCCCACCTGA GACCAAAGCC TGTGTATGTC 
ACTACCACTC GAGACAATGA GAGCATTTAC AGCACAAAGA TCCCATACAT GGCAGCTCGT 
GTCGTCTTCA TTAAGTGGAT GGTGACTTTC TTTTTGGAAA AAAAGTATCT AACTGCAACA 
CAGAACACTA AGAATGGAGT TGATGTATTG CCTAAAATCA TTCAGACCGT CGGGGGAGGC 
ACTGCAGTGC CCGAGCGGGT GCCGGAGCCA GACGCTGGCG GGGCGGCAGA GCAGGACCGA 
GGCCTCACGG ATGGGAGCAC CCTGTGCGAG CGCCGGCTCA GCGGCTGCAG CCTGTGCGGC 
ATTGAGGAGG AGCACCGTGC GGTTTACGAC ATGGTCCAGC GCATCCTGCT GTCCACCCGC 
GGCTATGTCA ACTTCGTGAA CGAGGTGTTC CGCCAGGCAT TTTTGTTGCC TTCGTGCGAC 
ATAGCCGTAA CCAGAAAAGT AGTTCAAGTG TACAGAAAGT GGATCCTGCA GGATAAACCT 
GTGTTCATGG AGGAACCAGA CATAAAAGAA GCTGCCCAAG AAGATGCTGA AAAATTAGGA 
TTTTCAGAGA CTGATAACAA GCAGACCTCT TCTGAAGGTT CCAGTCACAA GCGGTCTTCC 
AGTTGGGGAC GGTCTTACTC CTTCACCAGT GCCATGAGCA GAGGGTGTGT GATGGAAGAG 
GAGAATAAGA ACGTGAAGGC TGGGGCCCAG GCAATGCTGC AGGTATTTCT GACAAATGCT 
GCAAACGTCT TTTTGCTGGA GCCTTGTGTG GAGGTTCCCA TGCTCTTGAA GGAGCAAGTT 
GATGCCTGCA AAGCTGTTCT GATTATCTTT AGGCGCATGA TAATGGAGCT GACAATGAAT 
AAAACGACCT GGGAACAGAT GTTGCAAATA CTGCTCAGGA TAACAGAAGC TGTCATGCAG 
AAGCCAAAGG ATAAACAAAT CAAGGACTTG TTTGCCCAGA GCTTGGCAGG GTTACTGTTT 
AGGACGCTCA TAGTGGCCTG GATCCGAGCA AACCTCTGTG TGTACGTCTC CCGGGAGCTC 
TGGGACGACT TTCTCGGGGT GCTCTCCTCC CTCACGGACT GGGAGGAGCT GATCCACGAA 
TGGGCCAGCA TCATGGACTC CTTGACGGCT GTGCTGGCCA GGACCGTGTA TGGAGTTGAG 
ATGACCAATC TACCTCTGGA CAAGTTAAGT GAACAGAAGG AAAAGAAACA GCGAGGCAAA 
GGCTGTGTTC CAGATTCTCA GAAAGGAAGC ACCGTGGGGA GGTCCTTTTC TCTGAGCTGG 
CGGAGCCACC CCGACGTGGC CGTGGCCGAG CCCATGAGAT TCAGGAGCGC CACCACGTCC 
GGGGCGCCAG GGGTCGAGAA AGCCAGAAAC GTTGTCCGCC AGAAGGCAAC CGAAGTGGAA 
GAATACCTGC AGCCAGAAAA CACACCTGCC ACCGAATCTG GGCCCCTGGG GCAGCAGCCG 
ATGGTTCGGA GCGGCAGCAG CCCTGAGGTC ACCGAGCCGC TGTGTCCTGA GCCCCCCCCA 
GGCCAAAAGC TAGAAAATGC ACAGAACGTG AGTTCTTCAG AGCCCAGACT TGCTCAGGAG 
AGCAAAGGAC ATGAGAAGAA AGAGCATGAA GGAATAACAA TCTTAGTTCG AAGAAGCAGC 
AGCCCCATTG AACTGGATTT GAAAGATGAC TCACAGCAGA TGCAAGGAAA ACGTAGGGAG 
AGACAGAAGA GTGACAGTGT GAGCAGTGAC CCCACTCTGG GCTGCAGCAC TGAGGCAGAG 
CTGTCCCTGA GCCACTGGCA GACCTGCGAG GAAGACCCAG ATCTGAGCCC GCCTGCAGAT 
ACTGTGACCG ACGCTGACGC CCGCCACTGG TTACAGCTCC GGCCCACGGA TGCTTCAAAC 
CTAACAGACA GCAGCGAATG CCTTGCTGAT GACTGCAGTA TAATTGCTGG GGGGACCCTC 
ACTGGTTGGC ACCCAGACTC AGCCGCTGTG TTGTGGCGAA GAATCTTGGG AATCCTTGGA 
GATGTGAATA ATATCCAGTC ACCCAAAATC CATGCCAGGG TGTTTGGCTA TCTCTATGAA 
CTCTGGTACA AGCTAGCAAA GATACGGGAT AACCTGGCAA TAAGCCTGGA TAACCAGTCT 
TCTCCATCTC CTCCTGCCTT GATCCCACCG CTCAGAATGT TTGCATCATG GCTATTTAAG 
GCGACGACGA TGCCTGACGA TTATAAGGAA GGCAAACTGC AAGCCTATAA GCTGATCTGC 
TCCATGATGA CCCGGCGCCA GGACGTCCTG CCCAACTCAG ACTTCCTGGT GCATTTTTAC 
CTCGTCATGC ACCTGGGACT GACCAGCGAG GACCAGGATA TCCTGAATAC CATCATAAGG 
CACTGCCCGC CCCGCTTCTT CTCCCTGGGC TTGCCTGGCT TCTCCATGCT GGTGGGGGAC 
TTCATCATGG CTGCTGGCAG GGTCCTCAGT GCAGACACGC TGGCGGCACC TCGTTCGGAA 
GCCCTCACCA TTCTCGGGTC TCTGGTCTGC TTTCCGAACA CCTACCGCGA GATTCCCCTA 
CGGCAGCCGG TACCTGAAGT TGCTGAAGTC GCCATGGGAG CAGAAGATGT CAAGCACTGC 
CTCATAAGTA TCTTACTGAA GAATGCCACA GAGGAACCGA GTGAGTGTGC AAGGTGCATT 
GCCATCTGCT CCCTCGGGAT CTGGATCTGT GAGGAGCTGG CACAAGGCAC TGGCCACCCT 
CAGCTGCAAG AGGCCGTCAA CGTCATTGGT GTCACGTTGA AGTTTCCTAA CAAAATTGTG 
GCCCAGGTAG CCTGTGACGT GCTTCAGCTG CTAGTCTCCT ACTGGGAAAA GCTTCAGATG 
TTTGAAACCT CCTTGCCTCG GAAAATTGCA GAAATCCTTG TGGCCACAAT TGCTTTTCTG 
TTACCAAATG CAGAGTACTC CTCGGTGGAG ACAGACAAGA AGTTCCTCGT GTCCTTGTTG 
CTCTGCCTCC TGGACTGGTG CATGGCGCTG CCGGCAGGAA CCCTCCTCCA CCCCGTGTCC 
ACGGCGGCCC TGGACGAACA GGCCCCGGCC CGGGCCCCCT TACTGGATTA CATCTACAGG 
GTTCTGCACT GCTGCATCTG TGGCTCAAGC ACGTACAGCC AGCAGAGCCG CTACACCTTG 
ACCCTGGCGG ACCTGTCGTC CTCGGATTAC GACCCCTTCC TGCCGCTGGC GAACGTGAAG 
AATTCTGAGC CGGTCCAATT TCATTCATCA GCAGAACTGG GCAACCTGCT CACTGTTGAA 
GAGGAGAGGA GGAGAAGGAG CCTGGGGCTG ATCCCGCTGA CAGCGCGCAT GGTGATGGCG 
CACCTGGTCA ACCACCTGGG CCACTTCCCG CTCTGCGGGG GCCCCGCCGT GCTGCACAGC 
CTGGTGGGCG AGCACCACGA CAACGTGCAC GCCGACGCCG CCGAGCTCTC CCCAGACGTG 
TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG TTCAACGACA GCACCCTCAT CTCCTACCTG 
CAGACGCCTG CGACCGGTCC TGCCAGTGGG TCGCCAGCGG GCGCCCCCTC GGACGTGAGA 
GTGATCGTGA GGGACATCTC AGGGAAGTAC TCGTGGGACG GGAAGGTGCT CTACGGACCC 
CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA AGGCCCTCGT TCCTCATCTC GAGCTGTCAA 
CCCCCAACCC CTGGACCTCA GAGGGCTCCT TCTCAGACTG AGGAGGGAGA GGATGTCCTC 
GACAAATTAC TTGAAAACCT TGGCCACACG AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC 
CGTCTGAATG AGCCTTCCCC ACCCCCTTGT GGCATGAATT GGGACCAGGA GAAGGAGATC 
ACCAAGGTCC TGCTGCGCCA GAGCACGCAA GAAGACGAGT ATGTCCAGAG GTGCAACTCG 
GATGTGGCCA TGAGGGTCGC CAGCCAGGAG CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC 
TTCTACTTCT GTAGGCTGCT GCTCGATGAC TTGGGAATGA ATTCTTGGGA CAGAAGGAAG 
AACTTCCATC TGTTGAAGAA AAATTCAAAA TTATTGAGAG AGCTAAAAAA TCTGGACTCC 
CGGCAGTGCC GTGAAACGCA CAAAATCGCA GTGTTTTACA TTGCCGAAGG TCAAGAAGAC 
AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA AGCCAAGCGT ATGAAGACTT TGTTGCTGGA 
CTTGGATGGG AGGTGGACCT GTCAACCCAC TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT 
GGCAGCACGG GCCAGACCGC CCCCTACTAC GCTACCTCGA CCGTGGAAGT CATCTTCCAC 
GTCTCAACTC GGATGCCGTC AGACTCAGAC GACTCGCTCA CCAAAAAGCT CCGTCACTTG 
GGGAATGACG AAGTCCACAT CGTCTGGTCC GAGCACTCCA GGGACTACCG CAGCGGGATC 
ATCCCCACCG CCTTCGGAGA CGTTTCAATC ATCATCTACC CCATGAAGAA TCACATGTTC 
TTTATCGCGA TAACCAAGAA ACCCGAGGTT CCATTTTTTG GGCCTCTGTT TGATGGAGCA 
ATCGTGAGTG GGAAGCTACT GCCAAGCCTT ATCTGTGCCA CCTGCATCAA CGCCAGCCGG 
GCCGTCAAGT GCCTCATCCC CCTCTACCAG AGCTTCTACG AGGAGAGAGC TCTCTATCTG 
GAAGCAATAA TTCAGAACCA CCGTGAAGTC ATGACATTTG AGGATTTCAC AGCCCAGGTC 
TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC GGAACTGTGT CAGCTCTGGA AGAGGGAAGG 
GATGGATGTG ATGAACATCT GCTCCACCTG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_025001045.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856813.1 Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X6, mRNA.

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CloneID	OBa221914
Clone ID Related Accession (Same CDS sequence)	XM_025001045.1
Accession Version	XM_025001045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA 
TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA 
TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT 
AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG 
TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG 
AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT 
CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC 
CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC 
ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG 
CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG 
CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG 
GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT 
CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA 
CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC 
CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA 
AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT 
CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG 
GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC 
GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC 
ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT 
TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG 
ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA 
GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC 
AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA 
GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG 
GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG 
CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA 
ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA 
ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC 
TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG 
TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG 
GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG 
ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA 
AAGAAACAGC GAGGCAAAGA AGTGGAAGAA TACCTGCAGC CAGAAAACAC ACCTGCCACC 
GAATCTGGGC CCCTGGGGCA GCAGCCGATG GTTCGGAGCG GCAGCAGCCC TGAGGTCACC 
GAGCCGCTGT GTCCTGAGCC CCCCCCAGGC CAAAAGCTAG AAAATGCACA GAACGTGAGT 
TCTTCAGAGC CCAGACTTGC TCAGGAGAGC AAAGGACATG AGAAGAAAGA GCATGAAGGA 
ATAACAATCT TAGTTCGAAG AAGCAGCAGC CCCATTGAAC TGGATTTGAA AGATGACTCA 
CAGCAGATGC AAGGAAAACG TAGGGAGAGA CAGAAGAGTG ACAGTGTGAG CAGTGACCCC 
ACTCTGGGCT GCAGCACTGA GGCAGAGCTG TCCCTGAGCC ACTGGCAGAC CTGCGAGGAA 
GACCCAGATC TGAGCCCGCC TGCAGATACT GTGACCGACG CTGACGCCCG CCACTGGTTA 
CAGCTCCGGC CCACGGATGC TTCAAACCTA ACAGACAGCA GCGAATGCCT TGCTGATGAC 
TGCAGTATAA TTGCTGGGGG GACCCTCACT GGTTGGCACC CAGACTCAGC CGCTGTGTTG 
TGGCGAAGAA TCTTGGGAAT CCTTGGAGAT GTGAATAATA TCCAGTCACC CAAAATCCAT 
GCCAGGGTGT TTGGCTATCT CTATGAACTC TGGTACAAGC TAGCAAAGAT ACGGGATAAC 
CTGGCAATAA GCCTGGATAA CCAGTCTTCT CCATCTCCTC CTGCCTTGAT CCCACCGCTC 
AGAATGTTTG CATCATGGCT ATTTAAGGCG ACGACGATGC CTGACGATTA TAAGGAAGGC 
AAACTGCAAG CCTATAAGCT GATCTGCTCC ATGATGACCC GGCGCCAGGA CGTCCTGCCC 
AACTCAGACT TCCTGGTGCA TTTTTACCTC GTCATGCACC TGGGACTGAC CAGCGAGGAC 
CAGGATATCC TGAATACCAT CATAAGGCAC TGCCCGCCCC GCTTCTTCTC CCTGGGCTTG 
CCTGGCTTCT CCATGCTGGT GGGGGACTTC ATCATGGCTG CTGGCAGGGT CCTCAGTGCA 
GACACGCTGG CGGCACCTCG TTCGGAAGCC CTCACCATTC TCGGGTCTCT GGTCTGCTTT 
CCGAACACCT ACCGCGAGAT TCCCCTACGG CAGCCGGTAC CTGAAGTTGC TGAAGTCGCC 
ATGGGAGCAG AAGATGTCAA GCACTGCCTC ATAAGTATCT TACTGAAGAA TGCCACAGAG 
GAACCGAGTG AGTGTGCAAG GTGCATTGCC ATCTGCTCCC TCGGGATCTG GATCTGTGAG 
GAGCTGGCAC AAGGCACTGG CCACCCTCAG CTGCAAGAGG CCGTCAACGT CATTGGTGTC 
ACGTTGAAGT TTCCTAACAA AATTGTGGCC CAGGTAGCCT GTGACGTGCT TCAGCTGCTA 
GTCTCCTACT GGGAAAAGCT TCAGATGTTT GAAACCTCCT TGCCTCGGAA AATTGCAGAA 
ATCCTTGTGG CCACAATTGC TTTTCTGTTA CCAAATGCAG AGTACTCCTC GGTGGAGACA 
GACAAGAAGT TCCTCGTGTC CTTGTTGCTC TGCCTCCTGG ACTGGTGCAT GGCGCTGCCG 
GCAGGAACCC TCCTCCACCC CGTGTCCACG GCGGCCCTGG ACGAACAGGC CCCGGCCCGG 
GCCCCCTTAC TGGATTACAT CTACAGGGTT CTGCACTGCT GCATCTGTGG CTCAAGCACG 
TACAGCCAGC AGAGCCGCTA CACCTTGACC CTGGCGGACC TGTCGTCCTC GGATTACGAC 
CCCTTCCTGC CGCTGGCGAA CGTGAAGAAT TCTGAGCCGG TCCAATTTCA TTCATCAGCA 
GAACTGGGCA ACCTGCTCAC TGTTGAAGAG GCAGAGAGGA GGAGAAGGAG CCTGGGGCTG 
ATCCCGCTGA CAGCGCGCAT GGTGATGGCG CACCTGGTCA ACCACCTGGG CCACTTCCCG 
CTCTGCGGGG GCCCCGCCGT GCTGCACAGC CTGGTGGGCG AGCACCACGA CAACGTGCAC 
GCCGACGCCG CCGAGCTCTC CCCAGACGTG TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG 
TTCAACGACA GCACCCTCAT CTCCTACCTG CAGACGCCTG CGACCGGTCC TGCCAGTGGG 
TCGCCAGCGG GCGCCCCCTC GGACGTGAGA GTGATCGTGA GGGACATCTC AGGGAAGTAC 
TCGTGGGACG GGAAGGTGCT CTACGGACCC CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA 
AGGCCCTCGT TCCTCATCTC GAGCTGTCAA CCCCCAACCC CTGGACCTCA GAGGGCTCCT 
TCTCAGACTG AGGAGGGAGA GGATGTCCTC GACAAATTAC TTGAAAACCT TGGCCACACG 
AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC CGTCTGAATG AGCCTTCCCC ACCCCCTTGT 
GGCATGAATT GGGACCAGGA GAAGGAGATC ACCAAGGTCC TGCTGCGCCA GAGCACGCAA 
GAAGACGAGT ATGTCCAGAG GTGCAACTCG GATGTGGCCA TGAGGGTCGC CAGCCAGGAG 
CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC TTCTACTTCT GTAGGCTGCT GCTCGATGAC 
TTGGGAATGA ATTCTTGGGA CAGAAGGAAG AACTTCCATC TGTTGAAGAA AAATTCAAAA 
TTATTGAGAG AGCTAAAAAA TCTGGACTCC CGGCAGTGCC GTGAAACGCA CAAAATCGCA 
GTGTTTTACA TTGCCGAAGG TCAAGAAGAC AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA 
AGCCAAGCGT ATGAAGACTT TGTTGCTGGA CTTGGATGGG AGGTGGACCT GTCAACCCAC 
TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT GGCAGCACGG GCCAGACCGC CCCCTACTAC 
GCTACCTCGA CCGTGGAAGT CATCTTCCAC GTCTCAACTC GGATGCCGTC AGACTCAGAC 
GACTCGCTCA CCAAAAAGCT CCGTCACTTG GGGAATGACG AAGTCCACAT CGTCTGGTCC 
GAGCACTCCA GGGACTACCG CAGCGGGATC ATCCCCACCG CCTTCGGAGA CGTTTCAATC 
ATCATCTACC CCATGAAGAA TCACATGTTC TTTATCGCGA TAACCAAGAA ACCCGAGGTT 
CCATTTTTTG GGCCTCTGTT TGATGGAGCA ATCGTGAGTG GGAAGCTACT GCCAAGCCTT 
ATCTGTGCCA CCTGCATCAA CGCCAGCCGG GCCGTCAAGT GCCTCATCCC CCTCTACCAG 
AGCTTCTACG AGGAGAGAGC TCTCTATCTG GAAGCAATAA TTCAGAACCA CCGTGAAGTC 
ATGACATTTG AGGATTTCAC AGCCCAGGTC TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC 
GGAACTGTGT CAGCTCTGGA AGAGGGAAGG GATGGATGTG ATGAACATCT GCTCCACCTG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856813.1
CDS	43..5568
Translation	MDQSQGTPVLPTKATLDQPVASGLPDMEAVSAKMSQVQPRSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIMAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADAAELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRRPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_025001045.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001045.1

ORF Insert Sequence:

ATGGACCAGA GCCAGGGTAC CCCAGTCCTC CCAACCAAGG CCACTCTGGA TCAACCAGTG 
GCTAGTGGAC TGCCAGATAT GGAAGCAGTC TCAGCCAAGA TGAGCCAAGT TCAACCCAGA 
TCAGACAATG TGGATGCCAG TGATCTTAAG CAGTTTTTTG AGACCAACTA TTCTCAGATA 
TATTTCATCT TCTATGAAAA TTTTATAACA CTGGAAAATA GTTTGAAATT AAAAGGGAAT 
AATAAGTCAC AAAGGGAGGA GCTGGACTCC ATTCTCTTTC TTTTTGAAAA AATATTGCAG 
TTTCTACCTG AACGAATTTT CTTTCGATGG CATTACCAGA GTATAGGCTC AACTTTAAAG 
AAGCTTCTAC ACACTGGAAA TTCTATTAAG ATAAGATGTG AAGGAATCCG GCTGTTTCTT 
CTGTGGCTTC AAGCACTTCA GACGAACTGT GCAGAAGAGC AGGTGCTGAT TTTTGCTAGC 
CTGGTGCCAG GATTCCCTGC CATCATGTCG TCCAGGGGGC CCTGTACACT GGACACGCTC 
ATCAACCCTG GCCCCAACAT GGCTGATGCG AAGATCCATC CGGAAGAAAT CACTCCACTG 
CTGCCAGCCG TATCAGGGGA GAAGATCGCC GAGGACCAAA CGTGCTTTTT TCTTCAAGTG 
CTGTTGAAAT ACATGGTTAC TCAGGCCGCA AGCTTGGAGT GGAAGCATAA GGAGAATCAG 
GAGACTGGGT TTAAATTTCT GTTTACGTTG TTTCGAAAGT ATTACCTTCC TCATCTGTTT 
CCCTCGTTTA CCAAGTCAAC CAACATCTAC AAGCCTGTGC TGGACATCCC CCACCTGAGA 
CCAAAGCCTG TGTATGTCAC TACCACTCGA GACAATGAGA GCATTTACAG CACAAAGATC 
CCATACATGG CAGCTCGTGT CGTCTTCATT AAGTGGATGG TGACTTTCTT TTTGGAAAAA 
AAGTATCTAA CTGCAACACA GAACACTAAG AATGGAGTTG ATGTATTGCC TAAAATCATT 
CAGACCGTCG GGGGAGGCAC TGCAGTGCCC GAGCGGGTGC CGGAGCCAGA CGCTGGCGGG 
GCGGCAGAGC AGGACCGAGG CCTCACGGAT GGGAGCACCC TGTGCGAGCG CCGGCTCAGC 
GGCTGCAGCC TGTGCGGCAT TGAGGAGGAG CACCGTGCGG TTTACGACAT GGTCCAGCGC 
ATCCTGCTGT CCACCCGCGG CTATGTCAAC TTCGTGAACG AGGTGTTCCG CCAGGCATTT 
TTGTTGCCTT CGTGCGACAT AGCCGTAACC AGAAAAGTAG TTCAAGTGTA CAGAAAGTGG 
ATCCTGCAGG ATAAACCTGT GTTCATGGAG GAACCAGACA TAAAAGAAGC TGCCCAAGAA 
GATGCTGAAA AATTAGGATT TTCAGAGACT GATAACAAGC AGACCTCTTC TGAAGGTTCC 
AGTCACAAGC GGTCTTCCAG TTGGGGACGG TCTTACTCCT TCACCAGTGC CATGAGCAGA 
GGGTGTGTGA TGGAAGAGGA GAATAAGAAC GTGAAGGCTG GGGCCCAGGC AATGCTGCAG 
GTATTTCTGA CAAATGCTGC AAACGTCTTT TTGCTGGAGC CTTGTGTGGA GGTTCCCATG 
CTCTTGAAGG AGCAAGTTGA TGCCTGCAAA GCTGTTCTGA TTATCTTTAG GCGCATGATA 
ATGGAGCTGA CAATGAATAA AACGACCTGG GAACAGATGT TGCAAATACT GCTCAGGATA 
ACAGAAGCTG TCATGCAGAA GCCAAAGGAT AAACAAATCA AGGACTTGTT TGCCCAGAGC 
TTGGCAGGGT TACTGTTTAG GACGCTCATA GTGGCCTGGA TCCGAGCAAA CCTCTGTGTG 
TACGTCTCCC GGGAGCTCTG GGACGACTTT CTCGGGGTGC TCTCCTCCCT CACGGACTGG 
GAGGAGCTGA TCCACGAATG GGCCAGCATC ATGGACTCCT TGACGGCTGT GCTGGCCAGG 
ACCGTGTATG GAGTTGAGAT GACCAATCTA CCTCTGGACA AGTTAAGTGA ACAGAAGGAA 
AAGAAACAGC GAGGCAAAGA AGTGGAAGAA TACCTGCAGC CAGAAAACAC ACCTGCCACC 
GAATCTGGGC CCCTGGGGCA GCAGCCGATG GTTCGGAGCG GCAGCAGCCC TGAGGTCACC 
GAGCCGCTGT GTCCTGAGCC CCCCCCAGGC CAAAAGCTAG AAAATGCACA GAACGTGAGT 
TCTTCAGAGC CCAGACTTGC TCAGGAGAGC AAAGGACATG AGAAGAAAGA GCATGAAGGA 
ATAACAATCT TAGTTCGAAG AAGCAGCAGC CCCATTGAAC TGGATTTGAA AGATGACTCA 
CAGCAGATGC AAGGAAAACG TAGGGAGAGA CAGAAGAGTG ACAGTGTGAG CAGTGACCCC 
ACTCTGGGCT GCAGCACTGA GGCAGAGCTG TCCCTGAGCC ACTGGCAGAC CTGCGAGGAA 
GACCCAGATC TGAGCCCGCC TGCAGATACT GTGACCGACG CTGACGCCCG CCACTGGTTA 
CAGCTCCGGC CCACGGATGC TTCAAACCTA ACAGACAGCA GCGAATGCCT TGCTGATGAC 
TGCAGTATAA TTGCTGGGGG GACCCTCACT GGTTGGCACC CAGACTCAGC CGCTGTGTTG 
TGGCGAAGAA TCTTGGGAAT CCTTGGAGAT GTGAATAATA TCCAGTCACC CAAAATCCAT 
GCCAGGGTGT TTGGCTATCT CTATGAACTC TGGTACAAGC TAGCAAAGAT ACGGGATAAC 
CTGGCAATAA GCCTGGATAA CCAGTCTTCT CCATCTCCTC CTGCCTTGAT CCCACCGCTC 
AGAATGTTTG CATCATGGCT ATTTAAGGCG ACGACGATGC CTGACGATTA TAAGGAAGGC 
AAACTGCAAG CCTATAAGCT GATCTGCTCC ATGATGACCC GGCGCCAGGA CGTCCTGCCC 
AACTCAGACT TCCTGGTGCA TTTTTACCTC GTCATGCACC TGGGACTGAC CAGCGAGGAC 
CAGGATATCC TGAATACCAT CATAAGGCAC TGCCCGCCCC GCTTCTTCTC CCTGGGCTTG 
CCTGGCTTCT CCATGCTGGT GGGGGACTTC ATCATGGCTG CTGGCAGGGT CCTCAGTGCA 
GACACGCTGG CGGCACCTCG TTCGGAAGCC CTCACCATTC TCGGGTCTCT GGTCTGCTTT 
CCGAACACCT ACCGCGAGAT TCCCCTACGG CAGCCGGTAC CTGAAGTTGC TGAAGTCGCC 
ATGGGAGCAG AAGATGTCAA GCACTGCCTC ATAAGTATCT TACTGAAGAA TGCCACAGAG 
GAACCGAGTG AGTGTGCAAG GTGCATTGCC ATCTGCTCCC TCGGGATCTG GATCTGTGAG 
GAGCTGGCAC AAGGCACTGG CCACCCTCAG CTGCAAGAGG CCGTCAACGT CATTGGTGTC 
ACGTTGAAGT TTCCTAACAA AATTGTGGCC CAGGTAGCCT GTGACGTGCT TCAGCTGCTA 
GTCTCCTACT GGGAAAAGCT TCAGATGTTT GAAACCTCCT TGCCTCGGAA AATTGCAGAA 
ATCCTTGTGG CCACAATTGC TTTTCTGTTA CCAAATGCAG AGTACTCCTC GGTGGAGACA 
GACAAGAAGT TCCTCGTGTC CTTGTTGCTC TGCCTCCTGG ACTGGTGCAT GGCGCTGCCG 
GCAGGAACCC TCCTCCACCC CGTGTCCACG GCGGCCCTGG ACGAACAGGC CCCGGCCCGG 
GCCCCCTTAC TGGATTACAT CTACAGGGTT CTGCACTGCT GCATCTGTGG CTCAAGCACG 
TACAGCCAGC AGAGCCGCTA CACCTTGACC CTGGCGGACC TGTCGTCCTC GGATTACGAC 
CCCTTCCTGC CGCTGGCGAA CGTGAAGAAT TCTGAGCCGG TCCAATTTCA TTCATCAGCA 
GAACTGGGCA ACCTGCTCAC TGTTGAAGAG GCAGAGAGGA GGAGAAGGAG CCTGGGGCTG 
ATCCCGCTGA CAGCGCGCAT GGTGATGGCG CACCTGGTCA ACCACCTGGG CCACTTCCCG 
CTCTGCGGGG GCCCCGCCGT GCTGCACAGC CTGGTGGGCG AGCACCACGA CAACGTGCAC 
GCCGACGCCG CCGAGCTCTC CCCAGACGTG TTCAGGAGCC CCAGCCTGCA GCTGTTTGTG 
TTCAACGACA GCACCCTCAT CTCCTACCTG CAGACGCCTG CGACCGGTCC TGCCAGTGGG 
TCGCCAGCGG GCGCCCCCTC GGACGTGAGA GTGATCGTGA GGGACATCTC AGGGAAGTAC 
TCGTGGGACG GGAAGGTGCT CTACGGACCC CTGGAAGGCC ACTTAGCCCC CAAGGGCAGA 
AGGCCCTCGT TCCTCATCTC GAGCTGTCAA CCCCCAACCC CTGGACCTCA GAGGGCTCCT 
TCTCAGACTG AGGAGGGAGA GGATGTCCTC GACAAATTAC TTGAAAACCT TGGCCACACG 
AGTCCTGAAT GCCTTTTGCC ATCCCAGCTC CGTCTGAATG AGCCTTCCCC ACCCCCTTGT 
GGCATGAATT GGGACCAGGA GAAGGAGATC ACCAAGGTCC TGCTGCGCCA GAGCACGCAA 
GAAGACGAGT ATGTCCAGAG GTGCAACTCG GATGTGGCCA TGAGGGTCGC CAGCCAGGAG 
CCGCCGTCCC CAGTGGAGCC CCGGGGTGCC TTCTACTTCT GTAGGCTGCT GCTCGATGAC 
TTGGGAATGA ATTCTTGGGA CAGAAGGAAG AACTTCCATC TGTTGAAGAA AAATTCAAAA 
TTATTGAGAG AGCTAAAAAA TCTGGACTCC CGGCAGTGCC GTGAAACGCA CAAAATCGCA 
GTGTTTTACA TTGCCGAAGG TCAAGAAGAC AAGTGTTCGA TCCTTTCCAA TGAAAGGGGA 
AGCCAAGCGT ATGAAGACTT TGTTGCTGGA CTTGGATGGG AGGTGGACCT GTCAACCCAC 
TGTGGGTTCA TGGGCGGGCT TCAGCGCAAT GGCAGCACGG GCCAGACCGC CCCCTACTAC 
GCTACCTCGA CCGTGGAAGT CATCTTCCAC GTCTCAACTC GGATGCCGTC AGACTCAGAC 
GACTCGCTCA CCAAAAAGCT CCGTCACTTG GGGAATGACG AAGTCCACAT CGTCTGGTCC 
GAGCACTCCA GGGACTACCG CAGCGGGATC ATCCCCACCG CCTTCGGAGA CGTTTCAATC 
ATCATCTACC CCATGAAGAA TCACATGTTC TTTATCGCGA TAACCAAGAA ACCCGAGGTT 
CCATTTTTTG GGCCTCTGTT TGATGGAGCA ATCGTGAGTG GGAAGCTACT GCCAAGCCTT 
ATCTGTGCCA CCTGCATCAA CGCCAGCCGG GCCGTCAAGT GCCTCATCCC CCTCTACCAG 
AGCTTCTACG AGGAGAGAGC TCTCTATCTG GAAGCAATAA TTCAGAACCA CCGTGAAGTC 
ATGACATTTG AGGATTTCAC AGCCCAGGTC TTCTGTCCAT CTCCCAGCTA CTCGGTCAGC 
GGAACTGTGT CAGCTCTGGA AGAGGGAAGG GATGGATGTG ATGAACATCT GCTCCACCTG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474043.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329529.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.

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CloneID	OBa92268
Clone ID Related Accession (Same CDS sequence)	XM_015474043.1
Accession Version	XM_015474043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGTGTC AGCTCTGGAA 
GAGGGAAGAG ATGGATGTGA TGAACATCTG CTCCACCTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329529.1
CDS	16..5760
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015474043.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474043.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGTGTC AGCTCTGGAA 
GAGGGAAGAG ATGGATGTGA TGAACATCTG CTCCACCTGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329530.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.

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CloneID	OBa92269
Clone ID Related Accession (Same CDS sequence)	XM_015474044.1
Accession Version	XM_015474044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG AGAGGAGGAG AAGGAGCCTG GGGCTGATCC CGCTGACAGC GCGCATGGTG 
ATGGCGCACC TGGTCAACCA CCTGGGCCAC TTCCCGCTCT GCGGGGGCCC CGCCGTGCTG 
CACAGCCTGG TGGGCGAGCA CCACGACAAC GTGCACGCCG ACGCCACCGA GCTCTCCCCA 
GACGTGTTCA GGAGCCCCAG CCTGCAGCTG TTTGTGTTCA ACGACAGCAC CCTCATCTCC 
TACCTGCAGA CGCCTGCGAC CGGTCCTGCC AGTGGGTCGC CAGCGGGCGC CCCCTCGGAC 
GTGAGAGTGA TCGTGAGGGA CATCTCAGGG AAGTACTCGT GGGACGGGAA GGTGCTCTAC 
GGACCCCTGG AAGGCCACTT AGCCCCCAAG GGCAGAAGCC CCTCGTTCCT CATCTCGAGC 
TGTCAACCCC CAACCCCTGG ACCTCAGAGG GCTCCTTCTC AGACTGAGGA GGGAGAGGAT 
GTCCTCGACA AATTACTTGA AAACCTTGGC CACACGAGTC CTGAATGCCT TTTGCCATCC 
CAGCTCCGTC TGAATGAGCC TTCCCCACCC CCTTGTGGCA TGAATTGGGA CCAGGAGAAG 
GAGATCACCA AGGTCCTGCT GCGCCAGAGC ACGCAAGAAG ACGAGTATGT CCAGAGGTGC 
AACTCGGATG TGGCCATGAG GGTCGCCAGC CAGGAGCCGC CGTCCCCAGT GGAGCCCCGG 
GGCGCCTTCT ACTTCTGTAG GCTGCTGCTC GATGACTTGG GAATGAATTC TTGGGACAGA 
AGGAAGAACT TCCATCTGTT GAAGAAAAAT TCAAAATTAT TGAGAGAGCT AAAAAATCTG 
GACTCCCGGC AGTGCCGTGA AACGCACAAA ATCGCAGTGT TTTACATTGC CGAAGGTCAA 
GAAGACAAGT GTTCGATCCT TTCCAATGAA AGGGGAAGCC AAGCGTATGA AGACTTTGTT 
GCTGGACTTG GATGGGAGGT GGACCTGTCA ACCCACTGTG GGTTCATGGG CGGGCTTCAG 
CGCAATGGCA GCACGGGCCA GACCGCCCCC TACTACGCTA CCTCGACCGT GGAAGTCATC 
TTCCACGTCT CAACTCGGAT GCCGTCAGAC TCAGACGACT CGCTCACCAA AAAGCTCCGT 
CACTTGGGGA ATGACGAAGT CCACATCGTC TGGTCCGAGC ACTCCAGGGA CTACCGCAGC 
GGGATCATCC CCACCGCCTT CGGAGACGTT TCAATCATCA TCTACCCCAT GAAGAATCAC 
ATGTTCTTTA TCGCGATAAC CAAGAAACCC GAGGTTCCAT TTTTTGGGCC TCTGTTTGAT 
GGAGCAATCG TGAGTGGGAA GCTACTGCCA AGCCTTATCT GTGCCACCTG CATCAACGCC 
AGCCGGGCCG TCAAGTGCCT CATCCCCCTC TACCAGAGCT TCTACGAGGA GAGAGCTCTC 
TATCTGGAAG CAATAATTCA GAACCACCGT GAAGTCATGA CATTTGAGGA TTTCACAGCC 
CAGGTCTTCT GTCCATCTCC CAGCTACTCG GTCAGCGGAA CTGTGTCAGC TCTGGAAGAG 
GGAAGAGATG GATGTGATGA ACATCTGCTC CACCTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329530.1
CDS	16..5757
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015474044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474044.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG AGAGGAGGAG AAGGAGCCTG GGGCTGATCC CGCTGACAGC GCGCATGGTG 
ATGGCGCACC TGGTCAACCA CCTGGGCCAC TTCCCGCTCT GCGGGGGCCC CGCCGTGCTG 
CACAGCCTGG TGGGCGAGCA CCACGACAAC GTGCACGCCG ACGCCACCGA GCTCTCCCCA 
GACGTGTTCA GGAGCCCCAG CCTGCAGCTG TTTGTGTTCA ACGACAGCAC CCTCATCTCC 
TACCTGCAGA CGCCTGCGAC CGGTCCTGCC AGTGGGTCGC CAGCGGGCGC CCCCTCGGAC 
GTGAGAGTGA TCGTGAGGGA CATCTCAGGG AAGTACTCGT GGGACGGGAA GGTGCTCTAC 
GGACCCCTGG AAGGCCACTT AGCCCCCAAG GGCAGAAGCC CCTCGTTCCT CATCTCGAGC 
TGTCAACCCC CAACCCCTGG ACCTCAGAGG GCTCCTTCTC AGACTGAGGA GGGAGAGGAT 
GTCCTCGACA AATTACTTGA AAACCTTGGC CACACGAGTC CTGAATGCCT TTTGCCATCC 
CAGCTCCGTC TGAATGAGCC TTCCCCACCC CCTTGTGGCA TGAATTGGGA CCAGGAGAAG 
GAGATCACCA AGGTCCTGCT GCGCCAGAGC ACGCAAGAAG ACGAGTATGT CCAGAGGTGC 
AACTCGGATG TGGCCATGAG GGTCGCCAGC CAGGAGCCGC CGTCCCCAGT GGAGCCCCGG 
GGCGCCTTCT ACTTCTGTAG GCTGCTGCTC GATGACTTGG GAATGAATTC TTGGGACAGA 
AGGAAGAACT TCCATCTGTT GAAGAAAAAT TCAAAATTAT TGAGAGAGCT AAAAAATCTG 
GACTCCCGGC AGTGCCGTGA AACGCACAAA ATCGCAGTGT TTTACATTGC CGAAGGTCAA 
GAAGACAAGT GTTCGATCCT TTCCAATGAA AGGGGAAGCC AAGCGTATGA AGACTTTGTT 
GCTGGACTTG GATGGGAGGT GGACCTGTCA ACCCACTGTG GGTTCATGGG CGGGCTTCAG 
CGCAATGGCA GCACGGGCCA GACCGCCCCC TACTACGCTA CCTCGACCGT GGAAGTCATC 
TTCCACGTCT CAACTCGGAT GCCGTCAGAC TCAGACGACT CGCTCACCAA AAAGCTCCGT 
CACTTGGGGA ATGACGAAGT CCACATCGTC TGGTCCGAGC ACTCCAGGGA CTACCGCAGC 
GGGATCATCC CCACCGCCTT CGGAGACGTT TCAATCATCA TCTACCCCAT GAAGAATCAC 
ATGTTCTTTA TCGCGATAAC CAAGAAACCC GAGGTTCCAT TTTTTGGGCC TCTGTTTGAT 
GGAGCAATCG TGAGTGGGAA GCTACTGCCA AGCCTTATCT GTGCCACCTG CATCAACGCC 
AGCCGGGCCG TCAAGTGCCT CATCCCCCTC TACCAGAGCT TCTACGAGGA GAGAGCTCTC 
TATCTGGAAG CAATAATTCA GAACCACCGT GAAGTCATGA CATTTGAGGA TTTCACAGCC 
CAGGTCTTCT GTCCATCTCC CAGCTACTCG GTCAGCGGAA CTGTGTCAGC TCTGGAAGAG 
GGAAGAGATG GATGTGATGA ACATCTGCTC CACCTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474050.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329536.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329536.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474050.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474050.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474046.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329532.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329532.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474046.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474046.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474047.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329533.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329533.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474047.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474047.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474052.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329538.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329538.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474052.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474052.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474048.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329534.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329534.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474048.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474048.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474051.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329537.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329537.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474051.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474051.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474053.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329539.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329539.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474053.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474053.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474045.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329531.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329531.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474045.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474045.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474049.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329535.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.

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CloneID	OBa92270
Clone ID Related Accession (Same CDS sequence)	XM_015474050.1 , XM_015474046.1 , XM_015474047.1 , XM_015474052.1 , XM_015474048.1 , XM_015474051.1 , XM_015474053.1 , XM_015474045.1 , XM_015474049.1
Accession Version	XM_015474049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329535.1
CDS	16..5745
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015474049.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474049.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGGACA AGTACAGTCA 
AACCCAGACG AGCCAGGCAG CTTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474056.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329542.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.

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CloneID	OBa92271
Clone ID Related Accession (Same CDS sequence)	XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
Accession Version	XM_015474056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329542.1
CDS	16..5712
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015474056.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474056.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474055.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329541.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.

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CloneID	OBa92271
Clone ID Related Accession (Same CDS sequence)	XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
Accession Version	XM_015474055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329541.1
CDS	16..5712
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015474055.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474055.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474054.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329540.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.

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CloneID	OBa92271
Clone ID Related Accession (Same CDS sequence)	XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
Accession Version	XM_015474054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329540.1
CDS	16..5712
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015474054.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474054.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474057.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329543.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.

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CloneID	OBa92271
Clone ID Related Accession (Same CDS sequence)	XM_015474056.1 , XM_015474055.1 , XM_015474054.1 , XM_015474057.1
Accession Version	XM_015474057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329543.1
CDS	16..5712
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015474057.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474057.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGAACT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474060.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329546.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.

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CloneID	OBa92272
Clone ID Related Accession (Same CDS sequence)	XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
Accession Version	XM_015474060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329546.1
CDS	16..5706
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_015474060.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474060.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474061.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329547.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.

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CloneID	OBa92272
Clone ID Related Accession (Same CDS sequence)	XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
Accession Version	XM_015474061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329547.1
CDS	16..5706
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_015474061.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474061.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474058.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329544.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.

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CloneID	OBa92272
Clone ID Related Accession (Same CDS sequence)	XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
Accession Version	XM_015474058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329544.1
CDS	16..5706
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_015474058.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474058.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474059.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329545.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.

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CloneID	OBa92272
Clone ID Related Accession (Same CDS sequence)	XM_015474060.1 , XM_015474061.1 , XM_015474058.1 , XM_015474059.1
Accession Version	XM_015474059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329545.1
CDS	16..5706
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_015474059.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474059.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGCTA AGCGAAGCCA GTCTATCAGC 
AACTGTGCCC ACCTCTCTGA GGCTTTGCCC GCTACTAAAA GCGTCCCACT CCTCCTCCAC 
ACCGTGAGCG CTCTCTTCCC TGGTCTCTCT TACTCTTCTT GCTCCCACAG GCTGTCAGAA 
GTGGAAGAAT ACCTGCAGCC AGAAAACACA CCTGCCACCG AATCTGGGCC CCTGGGGCAG 
CAGCCGATGG TTCGGAGCGG CAGCAGCCCT GAGGTCACCG AGCCGCTGTG TCCTGAGCCC 
CCCCCAGGCC AAAAGCTAGA AAATGCACAG AACGTGAGTT CTTCAGAGCC CAGACTTGCT 
CAGGAGAGCA AAGGACATGA GAAGAAAGAG CATGAAGGAA TAACAATCTT AGTTCGAAGA 
AGCAGCAGCC CCATTGAACT GGATTTGAAA GATGACTCAC AGCAGATGCA AGGAAAACGT 
AGGGAGAGAC AGAAGAGTGA CAGTGTGAGC AGTGACCCCA CTCTGGGCTG CAGCACTGAG 
GCAGAGCTGT CCCTGAGCCA CTGGCAGACC TGCGAGGAAG ACCCAGATCT GAGCCCGCCT 
GCAGATACTG TGACCGACGC TGACGCCCGC CACTGGTTAC AGCTCCGGCC CACGGATGCT 
TCAAACCTAA CAGACAGCAG CGAATGCCTT GCTGATGACT GCAGTATAAT TGCTGGGGGG 
ACCCTCACTG GTTGGCACCC AGACTCAGCC GCTGTGTTGT GGCGAAGAAT CTTGGGAATC 
CTTGGAGATG TGAATAATAT CCAGTCACCC AAAATCCATG CCAGGGTGTT TGGCTATCTC 
TATGAACTCT GGTACAAGCT AGCAAAGATA CGGGATAACC TGGCAATAAG CCTGGATAAC 
CAGTCTTCTC CATCTCCTCC TGCCTTGATC CCACCGCTCA GAATGTTTGC ATCATGGCTA 
TTTAAGGCGA CGACGATGCC TGACGATTAT AAGGAAGGCA AACTGCAAGC CTATAAGCTG 
ATCTGCTCCA TGATGACCCG GCGCCAGGAC GTCCTGCCCA ACTCAGACTT CCTGGTGCAT 
TTTTACCTCG TCATGCACCT GGGACTGACC AGCGAGGACC AGGATATCCT GAATACCATC 
ATAAGGCACT GCCCGCCCCG CTTCTTCTCC CTGGGCTTGC CTGGCTTCTC CATGCTGGTG 
GGGGACTTCA TCGTGGCTGC TGGCAGGGTC CTCAGTGCAG ACACGCTGGC GGCACCTCGT 
TCGGAAGCCC TCACCATTCT CGGGTCTCTG GTCTGCTTTC CGAACACCTA CCGCGAGATT 
CCCCTACGGC AGCCGGTACC TGAAGTTGCT GAAGTCGCCA TGGGAGCAGA AGATGTCAAG 
CACTGCCTCA TAAGTATCTT ACTGAAGAAT GCCACAGAGG AACCGAGTGA GTGTGCAAGG 
TGCATTGCCA TCTGCTCCCT CGGGATCTGG ATCTGTGAGG AGCTGGCACA AGGCACTGGC 
CACCCTCAGC TGCAAGAGGC CGTCAACGTC ATTGGTGTCA CGTTGAAGTT TCCTAACAAA 
ATTGTGGCCC AGGTAGCCTG TGACGTGCTT CAGCTGCTAG TCTCCTACTG GGAAAAGCTT 
CAGATGTTTG AAACCTCCTT GCCTCGGAAA ATTGCAGAAA TCCTTGTGGC CACAATTGCT 
TTTCTGTTAC CAAATGCAGA GTACTCCTCG GTGGAGACAG ACAAGAAGTT CCTCGTGTCC 
TTGTTGCTCT GCCTCCTGGA CTGGTGCATG GCGCTGCCGG CAGGAACCCT CCTCCACCCC 
GTGTCCACGG CGGCCCTGGA CGAACAGGCC CCGGCCCGGG CCCCCTTACT GGATTACATC 
TACAGGGTTC TGCACTGCTG CATCTGTGGC TCAAGCACGT ACAGCCAGCA GAGCCGCTAC 
ACCTTGACCC TGGCGGACCT GTCGTCCTCG GATTACGACC CCTTCCTGCC GCTGGCGAAC 
GTGAAGAATT CTGAGCCGGT CCAATTTCAT TCATCAGCAG AACTGGGCAA CCTGCTCACT 
GTTGAAGAGG CAGAGAGGAG GAGAAGGAGC CTGGGGCTGA TCCCGCTGAC AGCGCGCATG 
GTGATGGCGC ACCTGGTCAA CCACCTGGGC CACTTCCCGC TCTGCGGGGG CCCCGCCGTG 
CTGCACAGCC TGGTGGGCGA GCACCACGAC AACGTGCACG CCGACGCCAC CGAGCTCTCC 
CCAGACGTGT TCAGGAGCCC CAGCCTGCAG CTGTTTGTGT TCAACGACAG CACCCTCATC 
TCCTACCTGC AGACGCCTGC GACCGGTCCT GCCAGTGGGT CGCCAGCGGG CGCCCCCTCG 
GACGTGAGAG TGATCGTGAG GGACATCTCA GGGAAGTACT CGTGGGACGG GAAGGTGCTC 
TACGGACCCC TGGAAGGCCA CTTAGCCCCC AAGGGCAGAA GCCCCTCGTT CCTCATCTCG 
AGCTGTCAAC CCCCAACCCC TGGACCTCAG AGGGCTCCTT CTCAGACTGA GGAGGGAGAG 
GATGTCCTCG ACAAATTACT TGAAAACCTT GGCCACACGA GTCCTGAATG CCTTTTGCCA 
TCCCAGCTCC GTCTGAATGA GCCTTCCCCA CCCCCTTGTG GCATGAATTG GGACCAGGAG 
AAGGAGATCA CCAAGGTCCT GCTGCGCCAG AGCACGCAAG AAGACGAGTA TGTCCAGAGG 
TGCAACTCGG ATGTGGCCAT GAGGGTCGCC AGCCAGGAGC CGCCGTCCCC AGTGGAGCCC 
CGGGGCGCCT TCTACTTCTG TAGGCTGCTG CTCGATGACT TGGGAATGAA TTCTTGGGAC 
AGAAGGAAGA ACTTCCATCT GTTGAAGAAA AATTCAAAAT TATTGAGAGA GCTAAAAAAT 
CTGGACTCCC GGCAGTGCCG TGAAACGCAC AAAATCGCAG TGTTTTACAT TGCCGAAGGT 
CAAGAAGACA AGTGTTCGAT CCTTTCCAAT GAAAGGGGAA GCCAAGCGTA TGAAGACTTT 
GTTGCTGGAC TTGGATGGGA GGTGGACCTG TCAACCCACT GTGGGTTCAT GGGCGGGCTT 
CAGCGCAATG GCAGCACGGG CCAGACCGCC CCCTACTACG CTACCTCGAC CGTGGAAGTC 
ATCTTCCACG TCTCAACTCG GATGCCGTCA GACTCAGACG ACTCGCTCAC CAAAAAGCTC 
CGTCACTTGG GGAATGACGA AGTCCACATC GTCTGGTCCG AGCACTCCAG GGACTACCGC 
AGCGGGATCA TCCCCACCGC CTTCGGAGAC GTTTCAATCA TCATCTACCC CATGAAGAAT 
CACATGTTCT TTATCGCGAT AACCAAGAAA CCCGAGGTTC CATTTTTTGG GCCTCTGTTT 
GATGGAGCAA TCGTGAGTGG GAAGCTACTG CCAAGCCTTA TCTGTGCCAC CTGCATCAAC 
GCCAGCCGGG CCGTCAAGTG CCTCATCCCC CTCTACCAGA GCTTCTACGA GGAGAGAGCT 
CTCTATCTGG AAGCAATAAT TCAGAACCAC CGTGAAGTCA TGACATTTGA GGATTTCACA 
GCCCAGGTCT TCTGTCCATC TCCCAGCTAC TCGGTCAGCG GAACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474062.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329548.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X20, mRNA.

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CloneID	OBa92273
Clone ID Related Accession (Same CDS sequence)	XM_015474062.1
Accession Version	XM_015474062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA 
GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC 
AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA 
AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG 
AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG 
GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC 
AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC 
TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT 
GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC 
GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA 
GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC 
CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA 
GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT 
GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT 
GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG 
CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG 
GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC 
TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT 
GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC 
GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT 
GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA 
CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC 
GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC 
GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT 
GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG 
CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG 
TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC 
TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC 
GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC 
ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG 
TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC 
CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGC AGAGAGGAGG 
AGAAGGAGCC TGGGGCTGAT CCCGCTGACA GCGCGCATGG TGATGGCGCA CCTGGTCAAC 
CACCTGGGCC ACTTCCCGCT CTGCGGGGGC CCCGCCGTGC TGCACAGCCT GGTGGGCGAG 
CACCACGACA ACGTGCACGC CGACGCCACC GAGCTCTCCC CAGACGTGTT CAGGAGCCCC 
AGCCTGCAGC TGTTTGTGTT CAACGACAGC ACCCTCATCT CCTACCTGCA GACGCCTGCG 
ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC GCCCCCTCGG ACGTGAGAGT GATCGTGAGG 
GACATCTCAG GGAAGTACTC GTGGGACGGG AAGGTGCTCT ACGGACCCCT GGAAGGCCAC 
TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC CTCATCTCGA GCTGTCAACC CCCAACCCCT 
GGACCTCAGA GGGCTCCTTC TCAGACTGAG GAGGGAGAGG ATGTCCTCGA CAAATTACTT 
GAAAACCTTG GCCACACGAG TCCTGAATGC CTTTTGCCAT CCCAGCTCCG TCTGAATGAG 
CCTTCCCCAC CCCCTTGTGG CATGAATTGG GACCAGGAGA AGGAGATCAC CAAGGTCCTG 
CTGCGCCAGA GCACGCAAGA AGACGAGTAT GTCCAGAGGT GCAACTCGGA TGTGGCCATG 
AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA GTGGAGCCCC GGGGCGCCTT CTACTTCTGT 
AGGCTGCTGC TCGATGACTT GGGAATGAAT TCTTGGGACA GAAGGAAGAA CTTCCATCTG 
TTGAAGAAAA ATTCAAAATT ATTGAGAGAG CTAAAAAATC TGGACTCCCG GCAGTGCCGT 
GAAACGCACA AAATCGCAGT GTTTTACATT GCCGAAGGTC AAGAAGACAA GTGTTCGATC 
CTTTCCAATG AAAGGGGAAG CCAAGCGTAT GAAGACTTTG TTGCTGGACT TGGATGGGAG 
GTGGACCTGT CAACCCACTG TGGGTTCATG GGCGGGCTTC AGCGCAATGG CAGCACGGGC 
CAGACCGCCC CCTACTACGC TACCTCGACC GTGGAAGTCA TCTTCCACGT CTCAACTCGG 
ATGCCGTCAG ACTCAGACGA CTCGCTCACC AAAAAGCTCC GTCACTTGGG GAATGACGAA 
GTCCACATCG TCTGGTCCGA GCACTCCAGG GACTACCGCA GCGGGATCAT CCCCACCGCC 
TTCGGAGACG TTTCAATCAT CATCTACCCC ATGAAGAATC ACATGTTCTT TATCGCGATA 
ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG 
AAGCTACTGC CAAGCCTTAT CTGTGCCACC TGCATCAACG CCAGCCGGGC CGTCAAGTGC 
CTCATCCCCC TCTACCAGAG CTTCTACGAG GAGAGAGCTC TCTATCTGGA AGCAATAATT 
CAGAACCACC GTGAAGTCAT GACATTTGAG GATTTCACAG CCCAGGTCTT CTGTCCATCT 
CCCAGCTACT CGGTCAGCGG AACTGTGTCA GCTCTGGAAG AGGGAAGAGA TGGATGTGAT 
GAACATCTGC TCCACCTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329548.1
CDS	16..5619
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015474062.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474062.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA 
GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC 
AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA 
AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG 
AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG 
GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC 
AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC 
TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT 
GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC 
GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA 
GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC 
CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA 
GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT 
GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT 
GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG 
CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG 
GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC 
TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT 
GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC 
GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT 
GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA 
CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC 
GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC 
GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT 
GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG 
CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG 
TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC 
TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC 
GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC 
ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG 
TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC 
CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGC AGAGAGGAGG 
AGAAGGAGCC TGGGGCTGAT CCCGCTGACA GCGCGCATGG TGATGGCGCA CCTGGTCAAC 
CACCTGGGCC ACTTCCCGCT CTGCGGGGGC CCCGCCGTGC TGCACAGCCT GGTGGGCGAG 
CACCACGACA ACGTGCACGC CGACGCCACC GAGCTCTCCC CAGACGTGTT CAGGAGCCCC 
AGCCTGCAGC TGTTTGTGTT CAACGACAGC ACCCTCATCT CCTACCTGCA GACGCCTGCG 
ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC GCCCCCTCGG ACGTGAGAGT GATCGTGAGG 
GACATCTCAG GGAAGTACTC GTGGGACGGG AAGGTGCTCT ACGGACCCCT GGAAGGCCAC 
TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC CTCATCTCGA GCTGTCAACC CCCAACCCCT 
GGACCTCAGA GGGCTCCTTC TCAGACTGAG GAGGGAGAGG ATGTCCTCGA CAAATTACTT 
GAAAACCTTG GCCACACGAG TCCTGAATGC CTTTTGCCAT CCCAGCTCCG TCTGAATGAG 
CCTTCCCCAC CCCCTTGTGG CATGAATTGG GACCAGGAGA AGGAGATCAC CAAGGTCCTG 
CTGCGCCAGA GCACGCAAGA AGACGAGTAT GTCCAGAGGT GCAACTCGGA TGTGGCCATG 
AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA GTGGAGCCCC GGGGCGCCTT CTACTTCTGT 
AGGCTGCTGC TCGATGACTT GGGAATGAAT TCTTGGGACA GAAGGAAGAA CTTCCATCTG 
TTGAAGAAAA ATTCAAAATT ATTGAGAGAG CTAAAAAATC TGGACTCCCG GCAGTGCCGT 
GAAACGCACA AAATCGCAGT GTTTTACATT GCCGAAGGTC AAGAAGACAA GTGTTCGATC 
CTTTCCAATG AAAGGGGAAG CCAAGCGTAT GAAGACTTTG TTGCTGGACT TGGATGGGAG 
GTGGACCTGT CAACCCACTG TGGGTTCATG GGCGGGCTTC AGCGCAATGG CAGCACGGGC 
CAGACCGCCC CCTACTACGC TACCTCGACC GTGGAAGTCA TCTTCCACGT CTCAACTCGG 
ATGCCGTCAG ACTCAGACGA CTCGCTCACC AAAAAGCTCC GTCACTTGGG GAATGACGAA 
GTCCACATCG TCTGGTCCGA GCACTCCAGG GACTACCGCA GCGGGATCAT CCCCACCGCC 
TTCGGAGACG TTTCAATCAT CATCTACCCC ATGAAGAATC ACATGTTCTT TATCGCGATA 
ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG 
AAGCTACTGC CAAGCCTTAT CTGTGCCACC TGCATCAACG CCAGCCGGGC CGTCAAGTGC 
CTCATCCCCC TCTACCAGAG CTTCTACGAG GAGAGAGCTC TCTATCTGGA AGCAATAATT 
CAGAACCACC GTGAAGTCAT GACATTTGAG GATTTCACAG CCCAGGTCTT CTGTCCATCT 
CCCAGCTACT CGGTCAGCGG AACTGTGTCA GCTCTGGAAG AGGGAAGAGA TGGATGTGAT 
GAACATCTGC TCCACCTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474063.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329549.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X21, mRNA.

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CloneID	OBa92274
Clone ID Related Accession (Same CDS sequence)	XM_015474063.1
Accession Version	XM_015474063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA 
GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC 
AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA 
AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG 
AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG 
GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC 
AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC 
TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT 
GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC 
GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA 
GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC 
CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA 
GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT 
GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT 
GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG 
CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG 
GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC 
TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT 
GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC 
GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT 
GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA 
CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC 
GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC 
GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT 
GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG 
CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG 
TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC 
TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC 
GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC 
ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG 
TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC 
CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGA GAGGAGGAGA 
AGGAGCCTGG GGCTGATCCC GCTGACAGCG CGCATGGTGA TGGCGCACCT GGTCAACCAC 
CTGGGCCACT TCCCGCTCTG CGGGGGCCCC GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC 
CACGACAACG TGCACGCCGA CGCCACCGAG CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC 
CTGCAGCTGT TTGTGTTCAA CGACAGCACC CTCATCTCCT ACCTGCAGAC GCCTGCGACC 
GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC CCCTCGGACG TGAGAGTGAT CGTGAGGGAC 
ATCTCAGGGA AGTACTCGTG GGACGGGAAG GTGCTCTACG GACCCCTGGA AGGCCACTTA 
GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC ATCTCGAGCT GTCAACCCCC AACCCCTGGA 
CCTCAGAGGG CTCCTTCTCA GACTGAGGAG GGAGAGGATG TCCTCGACAA ATTACTTGAA 
AACCTTGGCC ACACGAGTCC TGAATGCCTT TTGCCATCCC AGCTCCGTCT GAATGAGCCT 
TCCCCACCCC CTTGTGGCAT GAATTGGGAC CAGGAGAAGG AGATCACCAA GGTCCTGCTG 
CGCCAGAGCA CGCAAGAAGA CGAGTATGTC CAGAGGTGCA ACTCGGATGT GGCCATGAGG 
GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG 
CTGCTGCTCG ATGACTTGGG AATGAATTCT TGGGACAGAA GGAAGAACTT CCATCTGTTG 
AAGAAAAATT CAAAATTATT GAGAGAGCTA AAAAATCTGG ACTCCCGGCA GTGCCGTGAA 
ACGCACAAAA TCGCAGTGTT TTACATTGCC GAAGGTCAAG AAGACAAGTG TTCGATCCTT 
TCCAATGAAA GGGGAAGCCA AGCGTATGAA GACTTTGTTG CTGGACTTGG ATGGGAGGTG 
GACCTGTCAA CCCACTGTGG GTTCATGGGC GGGCTTCAGC GCAATGGCAG CACGGGCCAG 
ACCGCCCCCT ACTACGCTAC CTCGACCGTG GAAGTCATCT TCCACGTCTC AACTCGGATG 
CCGTCAGACT CAGACGACTC GCTCACCAAA AAGCTCCGTC ACTTGGGGAA TGACGAAGTC 
CACATCGTCT GGTCCGAGCA CTCCAGGGAC TACCGCAGCG GGATCATCCC CACCGCCTTC 
GGAGACGTTT CAATCATCAT CTACCCCATG AAGAATCACA TGTTCTTTAT CGCGATAACC 
AAGAAACCCG AGGTTCCATT TTTTGGGCCT CTGTTTGATG GAGCAATCGT GAGTGGGAAG 
CTACTGCCAA GCCTTATCTG TGCCACCTGC ATCAACGCCA GCCGGGCCGT CAAGTGCCTC 
ATCCCCCTCT ACCAGAGCTT CTACGAGGAG AGAGCTCTCT ATCTGGAAGC AATAATTCAG 
AACCACCGTG AAGTCATGAC ATTTGAGGAT TTCACAGCCC AGGTCTTCTG TCCATCTCCC 
AGCTACTCGG TCAGCGGAAC TGTGTCAGCT CTGGAAGAGG GAAGAGATGG ATGTGATGAA 
CATCTGCTCC ACCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329549.1
CDS	16..5616
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015474063.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474063.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGGCTG TGTTCCAGAT 
TCTCAGAAAG GAAGCACCGT GGGGAGGTCC TTTTCTCTGA GCTGGCGGAG CCACCCCGAC 
GTGGCCGTGG CCGAGCCCAT GAGATTCAGG AGCGCCACCA CGTCCGGGGC GCCAGGGGTC 
GAGAAAGCCA GAAACGTTGT CCGCCAGAAG GCAACCGAAG TGGAAGAATA CCTGCAGCCA 
GAAAACACAC CTGCCACCGA ATCTGGGCCC CTGGGGCAGC AGCCGATGGT TCGGAGCGGC 
AGCAGCCCTG AGGTCACCGA GCCGCTGTGT CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA 
AATGCACAGA ACGTGAGTTC TTCAGAGCCC AGACTTGCTC AGGAGAGCAA AGGACATGAG 
AAGAAAGAGC ATGAAGGAAT AACAATCTTA GTTCGAAGAA GCAGCAGCCC CATTGAACTG 
GATTTGAAAG ATGACTCACA GCAGATGCAA GGAAAACGTA GGGAGAGACA GAAGAGTGAC 
AGTGTGAGCA GTGACCCCAC TCTGGGCTGC AGCACTGAGG CAGAGCTGTC CCTGAGCCAC 
TGGCAGACCT GCGAGGAAGA CCCAGATCTG AGCCCGCCTG CAGATACTGT GACCGACGCT 
GACGCCCGCC ACTGGTTACA GCTCCGGCCC ACGGATGCTT CAAACCTAAC AGACAGCAGC 
GAATGCCTTG CTGATGACTG CAGTATAATT GCTGGGGGGA CCCTCACTGG TTGGCACCCA 
GACTCAGCCG CTGTGTTGTG GCGAAGAATC TTGGGAATCC TTGGAGATGT GAATAATATC 
CAGTCACCCA AAATCCATGC CAGGGTGTTT GGCTATCTCT ATGAACTCTG GTACAAGCTA 
GCAAAGATAC GGGATAACCT GGCAATAAGC CTGGATAACC AGTCTTCTCC ATCTCCTCCT 
GCCTTGATCC CACCGCTCAG AATGTTTGCA TCATGGCTAT TTAAGGCGAC GACGATGCCT 
GACGATTATA AGGAAGGCAA ACTGCAAGCC TATAAGCTGA TCTGCTCCAT GATGACCCGG 
CGCCAGGACG TCCTGCCCAA CTCAGACTTC CTGGTGCATT TTTACCTCGT CATGCACCTG 
GGACTGACCA GCGAGGACCA GGATATCCTG AATACCATCA TAAGGCACTG CCCGCCCCGC 
TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC ATGCTGGTGG GGGACTTCAT CGTGGCTGCT 
GGCAGGGTCC TCAGTGCAGA CACGCTGGCG GCACCTCGTT CGGAAGCCCT CACCATTCTC 
GGGTCTCTGG TCTGCTTTCC GAACACCTAC CGCGAGATTC CCCTACGGCA GCCGGTACCT 
GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA GATGTCAAGC ACTGCCTCAT AAGTATCTTA 
CTGAAGAATG CCACAGAGGA ACCGAGTGAG TGTGCAAGGT GCATTGCCAT CTGCTCCCTC 
GGGATCTGGA TCTGTGAGGA GCTGGCACAA GGCACTGGCC ACCCTCAGCT GCAAGAGGCC 
GTCAACGTCA TTGGTGTCAC GTTGAAGTTT CCTAACAAAA TTGTGGCCCA GGTAGCCTGT 
GACGTGCTTC AGCTGCTAGT CTCCTACTGG GAAAAGCTTC AGATGTTTGA AACCTCCTTG 
CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC ACAATTGCTT TTCTGTTACC AAATGCAGAG 
TACTCCTCGG TGGAGACAGA CAAGAAGTTC CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC 
TGGTGCATGG CGCTGCCGGC AGGAACCCTC CTCCACCCCG TGTCCACGGC GGCCCTGGAC 
GAACAGGCCC CGGCCCGGGC CCCCTTACTG GATTACATCT ACAGGGTTCT GCACTGCTGC 
ATCTGTGGCT CAAGCACGTA CAGCCAGCAG AGCCGCTACA CCTTGACCCT GGCGGACCTG 
TCGTCCTCGG ATTACGACCC CTTCCTGCCG CTGGCGAACG TGAAGAATTC TGAGCCGGTC 
CAATTTCATT CATCAGCAGA ACTGGGCAAC CTGCTCACTG TTGAAGAGGA GAGGAGGAGA 
AGGAGCCTGG GGCTGATCCC GCTGACAGCG CGCATGGTGA TGGCGCACCT GGTCAACCAC 
CTGGGCCACT TCCCGCTCTG CGGGGGCCCC GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC 
CACGACAACG TGCACGCCGA CGCCACCGAG CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC 
CTGCAGCTGT TTGTGTTCAA CGACAGCACC CTCATCTCCT ACCTGCAGAC GCCTGCGACC 
GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC CCCTCGGACG TGAGAGTGAT CGTGAGGGAC 
ATCTCAGGGA AGTACTCGTG GGACGGGAAG GTGCTCTACG GACCCCTGGA AGGCCACTTA 
GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC ATCTCGAGCT GTCAACCCCC AACCCCTGGA 
CCTCAGAGGG CTCCTTCTCA GACTGAGGAG GGAGAGGATG TCCTCGACAA ATTACTTGAA 
AACCTTGGCC ACACGAGTCC TGAATGCCTT TTGCCATCCC AGCTCCGTCT GAATGAGCCT 
TCCCCACCCC CTTGTGGCAT GAATTGGGAC CAGGAGAAGG AGATCACCAA GGTCCTGCTG 
CGCCAGAGCA CGCAAGAAGA CGAGTATGTC CAGAGGTGCA ACTCGGATGT GGCCATGAGG 
GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG 
CTGCTGCTCG ATGACTTGGG AATGAATTCT TGGGACAGAA GGAAGAACTT CCATCTGTTG 
AAGAAAAATT CAAAATTATT GAGAGAGCTA AAAAATCTGG ACTCCCGGCA GTGCCGTGAA 
ACGCACAAAA TCGCAGTGTT TTACATTGCC GAAGGTCAAG AAGACAAGTG TTCGATCCTT 
TCCAATGAAA GGGGAAGCCA AGCGTATGAA GACTTTGTTG CTGGACTTGG ATGGGAGGTG 
GACCTGTCAA CCCACTGTGG GTTCATGGGC GGGCTTCAGC GCAATGGCAG CACGGGCCAG 
ACCGCCCCCT ACTACGCTAC CTCGACCGTG GAAGTCATCT TCCACGTCTC AACTCGGATG 
CCGTCAGACT CAGACGACTC GCTCACCAAA AAGCTCCGTC ACTTGGGGAA TGACGAAGTC 
CACATCGTCT GGTCCGAGCA CTCCAGGGAC TACCGCAGCG GGATCATCCC CACCGCCTTC 
GGAGACGTTT CAATCATCAT CTACCCCATG AAGAATCACA TGTTCTTTAT CGCGATAACC 
AAGAAACCCG AGGTTCCATT TTTTGGGCCT CTGTTTGATG GAGCAATCGT GAGTGGGAAG 
CTACTGCCAA GCCTTATCTG TGCCACCTGC ATCAACGCCA GCCGGGCCGT CAAGTGCCTC 
ATCCCCCTCT ACCAGAGCTT CTACGAGGAG AGAGCTCTCT ATCTGGAAGC AATAATTCAG 
AACCACCGTG AAGTCATGAC ATTTGAGGAT TTCACAGCCC AGGTCTTCTG TCCATCTCCC 
AGCTACTCGG TCAGCGGAAC TGTGTCAGCT CTGGAAGAGG GAAGAGATGG ATGTGATGAA 
CATCTGCTCC ACCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015474064.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329550.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.

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CloneID	OBa92275
Clone ID Related Accession (Same CDS sequence)	XM_015474064.1
Accession Version	XM_015474064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGAAGT GGAAGAATAC 
CTGCAGCCAG AAAACACACC TGCCACCGAA TCTGGGCCCC TGGGGCAGCA GCCGATGGTT 
CGGAGCGGCA GCAGCCCTGA GGTCACCGAG CCGCTGTGTC CTGAGCCCCC CCCAGGCCAA 
AAGCTAGAAA ATGCACAGAA CGTGAGTTCT TCAGAGCCCA GACTTGCTCA GGAGAGCAAA 
GGACATGAGA AGAAAGAGCA TGAAGGAATA ACAATCTTAG TTCGAAGAAG CAGCAGCCCC 
ATTGAACTGG ATTTGAAAGA TGACTCACAG CAGATGCAAG GAAAACGTAG GGAGAGACAG 
AAGAGTGACA GTGTGAGCAG TGACCCCACT CTGGGCTGCA GCACTGAGGC AGAGCTGTCC 
CTGAGCCACT GGCAGACCTG CGAGGAAGAC CCAGATCTGA GCCCGCCTGC AGATACTGTG 
ACCGACGCTG ACGCCCGCCA CTGGTTACAG CTCCGGCCCA CGGATGCTTC AAACCTAACA 
GACAGCAGCG AATGCCTTGC TGATGACTGC AGTATAATTG CTGGGGGGAC CCTCACTGGT 
TGGCACCCAG ACTCAGCCGC TGTGTTGTGG CGAAGAATCT TGGGAATCCT TGGAGATGTG 
AATAATATCC AGTCACCCAA AATCCATGCC AGGGTGTTTG GCTATCTCTA TGAACTCTGG 
TACAAGCTAG CAAAGATACG GGATAACCTG GCAATAAGCC TGGATAACCA GTCTTCTCCA 
TCTCCTCCTG CCTTGATCCC ACCGCTCAGA ATGTTTGCAT CATGGCTATT TAAGGCGACG 
ACGATGCCTG ACGATTATAA GGAAGGCAAA CTGCAAGCCT ATAAGCTGAT CTGCTCCATG 
ATGACCCGGC GCCAGGACGT CCTGCCCAAC TCAGACTTCC TGGTGCATTT TTACCTCGTC 
ATGCACCTGG GACTGACCAG CGAGGACCAG GATATCCTGA ATACCATCAT AAGGCACTGC 
CCGCCCCGCT TCTTCTCCCT GGGCTTGCCT GGCTTCTCCA TGCTGGTGGG GGACTTCATC 
GTGGCTGCTG GCAGGGTCCT CAGTGCAGAC ACGCTGGCGG CACCTCGTTC GGAAGCCCTC 
ACCATTCTCG GGTCTCTGGT CTGCTTTCCG AACACCTACC GCGAGATTCC CCTACGGCAG 
CCGGTACCTG AAGTTGCTGA AGTCGCCATG GGAGCAGAAG ATGTCAAGCA CTGCCTCATA 
AGTATCTTAC TGAAGAATGC CACAGAGGAA CCGAGTGAGT GTGCAAGGTG CATTGCCATC 
TGCTCCCTCG GGATCTGGAT CTGTGAGGAG CTGGCACAAG GCACTGGCCA CCCTCAGCTG 
CAAGAGGCCG TCAACGTCAT TGGTGTCACG TTGAAGTTTC CTAACAAAAT TGTGGCCCAG 
GTAGCCTGTG ACGTGCTTCA GCTGCTAGTC TCCTACTGGG AAAAGCTTCA GATGTTTGAA 
ACCTCCTTGC CTCGGAAAAT TGCAGAAATC CTTGTGGCCA CAATTGCTTT TCTGTTACCA 
AATGCAGAGT ACTCCTCGGT GGAGACAGAC AAGAAGTTCC TCGTGTCCTT GTTGCTCTGC 
CTCCTGGACT GGTGCATGGC GCTGCCGGCA GGAACCCTCC TCCACCCCGT GTCCACGGCG 
GCCCTGGACG AACAGGCCCC GGCCCGGGCC CCCTTACTGG ATTACATCTA CAGGGTTCTG 
CACTGCTGCA TCTGTGGCTC AAGCACGTAC AGCCAGCAGA GCCGCTACAC CTTGACCCTG 
GCGGACCTGT CGTCCTCGGA TTACGACCCC TTCCTGCCGC TGGCGAACGT GAAGAATTCT 
GAGCCGGTCC AATTTCATTC ATCAGCAGAA CTGGGCAACC TGCTCACTGT TGAAGAGGCA 
GAGAGGAGGA GAAGGAGCCT GGGGCTGATC CCGCTGACAG CGCGCATGGT GATGGCGCAC 
CTGGTCAACC ACCTGGGCCA CTTCCCGCTC TGCGGGGGCC CCGCCGTGCT GCACAGCCTG 
GTGGGCGAGC ACCACGACAA CGTGCACGCC GACGCCACCG AGCTCTCCCC AGACGTGTTC 
AGGAGCCCCA GCCTGCAGCT GTTTGTGTTC AACGACAGCA CCCTCATCTC CTACCTGCAG 
ACGCCTGCGA CCGGTCCTGC CAGTGGGTCG CCAGCGGGCG CCCCCTCGGA CGTGAGAGTG 
ATCGTGAGGG ACATCTCAGG GAAGTACTCG TGGGACGGGA AGGTGCTCTA CGGACCCCTG 
GAAGGCCACT TAGCCCCCAA GGGCAGAAGC CCCTCGTTCC TCATCTCGAG CTGTCAACCC 
CCAACCCCTG GACCTCAGAG GGCTCCTTCT CAGACTGAGG AGGGAGAGGA TGTCCTCGAC 
AAATTACTTG AAAACCTTGG CCACACGAGT CCTGAATGCC TTTTGCCATC CCAGCTCCGT 
CTGAATGAGC CTTCCCCACC CCCTTGTGGC ATGAATTGGG ACCAGGAGAA GGAGATCACC 
AAGGTCCTGC TGCGCCAGAG CACGCAAGAA GACGAGTATG TCCAGAGGTG CAACTCGGAT 
GTGGCCATGA GGGTCGCCAG CCAGGAGCCG CCGTCCCCAG TGGAGCCCCG GGGCGCCTTC 
TACTTCTGTA GGCTGCTGCT CGATGACTTG GGAATGAATT CTTGGGACAG AAGGAAGAAC 
TTCCATCTGT TGAAGAAAAA TTCAAAATTA TTGAGAGAGC TAAAAAATCT GGACTCCCGG 
CAGTGCCGTG AAACGCACAA AATCGCAGTG TTTTACATTG CCGAAGGTCA AGAAGACAAG 
TGTTCGATCC TTTCCAATGA AAGGGGAAGC CAAGCGTATG AAGACTTTGT TGCTGGACTT 
GGATGGGAGG TGGACCTGTC AACCCACTGT GGGTTCATGG GCGGGCTTCA GCGCAATGGC 
AGCACGGGCC AGACCGCCCC CTACTACGCT ACCTCGACCG TGGAAGTCAT CTTCCACGTC 
TCAACTCGGA TGCCGTCAGA CTCAGACGAC TCGCTCACCA AAAAGCTCCG TCACTTGGGG 
AATGACGAAG TCCACATCGT CTGGTCCGAG CACTCCAGGG ACTACCGCAG CGGGATCATC 
CCCACCGCCT TCGGAGACGT TTCAATCATC ATCTACCCCA TGAAGAATCA CATGTTCTTT 
ATCGCGATAA CCAAGAAACC CGAGGTTCCA TTTTTTGGGC CTCTGTTTGA TGGAGCAATC 
GTGAGTGGGA AGCTACTGCC AAGCCTTATC TGTGCCACCT GCATCAACGC CAGCCGGGCC 
GTCAAGTGCC TCATCCCCCT CTACCAGAGC TTCTACGAGG AGAGAGCTCT CTATCTGGAA 
GCAATAATTC AGAACCACCG TGAAGTCATG ACATTTGAGG ATTTCACAGC CCAGGTCTTC 
TGTCCATCTC CCAGCTACTC GGTCAGCGGA ACTGTGTCAG CTCTGGAAGA GGGAAGAGAT 
GGATGTGATG AACATCTGCT CCACCTGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329550.1
CDS	16..5448
Translation	MVVNGNFFFSDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFASLVPGFPAIMSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015474064.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474064.1

ORF Insert Sequence:

ATGGTTGTAA ATGGGAACTT TTTCTTTTCA GACAATGTGG ATGCCAGTGA TCTTAAGCAG 
TTTTTTGAGA CCAACTATTC TCAGATATAT TTCATCTTCT ATGAAAATTT TATAACACTG 
GAAAATAGTT TGAAATTAAA AGGGAATAAT AAGTCACAAA GGGAGGAGCT GGACTCCATT 
CTCTTTCTTT TTGAAAAAAT ATTGCAGTTT CTACCTGAAC GAATTTTCTT TCGATGGCAT 
TACCAGAGTA TAGGCTCAAC TTTAAAGAAG CTTCTACACA CTGGAAATTC TATTAAGATA 
AGATGTGAAG GAATCCGGCT GTTTCTTCTG TGGCTTCAAG CACTTCAGAC GAACTGTGCA 
GAAGAGCAGG TGCTGATTTT TGCTAGCCTG GTGCCAGGAT TCCCTGCCAT CATGTCGTCC 
AGGGGGCCCT GTACACTGGA CACGCTCATC AACCCTGGCC CCAACATGGC TGATGCGAAG 
ATCCATCCGG AAGAAATCAC TCCACTGCTG CCAGCCGTAT CAGGGGAGAA GATCGCCGAG 
GACCAAACGT GCTTTTTTCT TCAAGTGCTG TTGAAATACA TGGTTACTCA GGCCGCAAGC 
TTGGAGTGGA AGCATAAGGA GAATCAGGAG ACTGGGTTTA AATTTCTGTT TACGTTGTTT 
CGAAAGTATT ACCTTCCTCA TCTGTTTCCC TCGTTTACCA AGTCAACCAA CATCTACAAG 
CCTGTGCTGG ACATCCCCCA CCTGAGACCA AAGCCTGTGT ATGTCACTAC CACTCGAGAC 
AATGAGAGCA TTTACAGCAC AAAGATCCCA TACATGGCAG CTCGTGTCGT CTTCATTAAG 
TGGATGGTGA CTTTCTTTTT GGAAAAAAAG TATCTAACTG CAACACAGAA CACTAAGAAT 
GGAGTTGATG TATTGCCTAA AATCATTCAG ACCGTCGGGG GAGGCACTGC AGTGCCCGAG 
CGGGTGCCGG AGCCAGACGC TGGCGGGGCG GCAGAGCAGG ACCGAGGCCT CACGGATGGG 
AGCACCCTGT GCGAGCGCCG GCTCAGCGGC TGCAGCCTGT GCGGCATTGA GGAGGAGCAC 
CGTGCGGTTT ACGACATGGT CCAGCGCATC CTGCTGTCCA CCCGCGGCTA TGTCAACTTC 
GTGAACGAGG TGTTCCGCCA GGCATTTTTG TTGCCTTCGT GCGACATAGC CGTAACCAGA 
AAAGTAGTTC AAGTGTACAG AAAGTGGATC CTGCAGGATA AACCTGTGTT CATGGAGGAA 
CCAGACATAA AAGAAGCTGC CCAAGAAGAT GCTGAAAAAT TAGGATTTTC AGAGACTGAT 
AACAAGCAGA CCTCTTCTGA AGGTTCCAGT CACAAGCGGT CTTCCAGTTG GGGACGGTCT 
TACTCCTTCA CCAGTGCCAT GAGCAGAGGG TGTGTGATGG AAGAGGAGAA TAAGAACGTG 
AAGGCTGGGG CCCAGGCAAT GCTGCAGGTA TTTCTGACAA ATGCTGCAAA CGTCTTTTTG 
CTGGAGCCTT GTGTGGAGGT TCCCATGCTC TTGAAGGAGC AAGTTGATGC CTGCAAAGCT 
GTTCTGATTA TCTTTAGGCG CATGATAATG GAGCTGACAA TGAATAAAAC GACCTGGGAA 
CAGATGTTGC AAATACTGCT CAGGATAACA GAAGCTGTCA TGCAGAAGCC AAAGGATAAA 
CAAATCAAGG ACTTGTTTGC CCAGAGCTTG GCAGGGTTAC TGTTTAGGAC GCTCATAGTG 
GCCTGGATCC GAGCAAACCT CTGTGTGTAC GTCTCCCGGG AGCTCTGGGA CGACTTTCTC 
GGGGTGCTCT CCTCCCTCAC GGACTGGGAG GAGCTGATCC ACGAATGGGC CAGCATCATG 
GACTCCTTGA CGGCTGTGCT GGCCAGGACC GTGTATGGAG TTGAGATGAC CAATCTACCT 
CTGGACAAGT TAAGTGAACA GAAGGAAAAG AAACAGCGAG GCAAAGAAGT GGAAGAATAC 
CTGCAGCCAG AAAACACACC TGCCACCGAA TCTGGGCCCC TGGGGCAGCA GCCGATGGTT 
CGGAGCGGCA GCAGCCCTGA GGTCACCGAG CCGCTGTGTC CTGAGCCCCC CCCAGGCCAA 
AAGCTAGAAA ATGCACAGAA CGTGAGTTCT TCAGAGCCCA GACTTGCTCA GGAGAGCAAA 
GGACATGAGA AGAAAGAGCA TGAAGGAATA ACAATCTTAG TTCGAAGAAG CAGCAGCCCC 
ATTGAACTGG ATTTGAAAGA TGACTCACAG CAGATGCAAG GAAAACGTAG GGAGAGACAG 
AAGAGTGACA GTGTGAGCAG TGACCCCACT CTGGGCTGCA GCACTGAGGC AGAGCTGTCC 
CTGAGCCACT GGCAGACCTG CGAGGAAGAC CCAGATCTGA GCCCGCCTGC AGATACTGTG 
ACCGACGCTG ACGCCCGCCA CTGGTTACAG CTCCGGCCCA CGGATGCTTC AAACCTAACA 
GACAGCAGCG AATGCCTTGC TGATGACTGC AGTATAATTG CTGGGGGGAC CCTCACTGGT 
TGGCACCCAG ACTCAGCCGC TGTGTTGTGG CGAAGAATCT TGGGAATCCT TGGAGATGTG 
AATAATATCC AGTCACCCAA AATCCATGCC AGGGTGTTTG GCTATCTCTA TGAACTCTGG 
TACAAGCTAG CAAAGATACG GGATAACCTG GCAATAAGCC TGGATAACCA GTCTTCTCCA 
TCTCCTCCTG CCTTGATCCC ACCGCTCAGA ATGTTTGCAT CATGGCTATT TAAGGCGACG 
ACGATGCCTG ACGATTATAA GGAAGGCAAA CTGCAAGCCT ATAAGCTGAT CTGCTCCATG 
ATGACCCGGC GCCAGGACGT CCTGCCCAAC TCAGACTTCC TGGTGCATTT TTACCTCGTC 
ATGCACCTGG GACTGACCAG CGAGGACCAG GATATCCTGA ATACCATCAT AAGGCACTGC 
CCGCCCCGCT TCTTCTCCCT GGGCTTGCCT GGCTTCTCCA TGCTGGTGGG GGACTTCATC 
GTGGCTGCTG GCAGGGTCCT CAGTGCAGAC ACGCTGGCGG CACCTCGTTC GGAAGCCCTC 
ACCATTCTCG GGTCTCTGGT CTGCTTTCCG AACACCTACC GCGAGATTCC CCTACGGCAG 
CCGGTACCTG AAGTTGCTGA AGTCGCCATG GGAGCAGAAG ATGTCAAGCA CTGCCTCATA 
AGTATCTTAC TGAAGAATGC CACAGAGGAA CCGAGTGAGT GTGCAAGGTG CATTGCCATC 
TGCTCCCTCG GGATCTGGAT CTGTGAGGAG CTGGCACAAG GCACTGGCCA CCCTCAGCTG 
CAAGAGGCCG TCAACGTCAT TGGTGTCACG TTGAAGTTTC CTAACAAAAT TGTGGCCCAG 
GTAGCCTGTG ACGTGCTTCA GCTGCTAGTC TCCTACTGGG AAAAGCTTCA GATGTTTGAA 
ACCTCCTTGC CTCGGAAAAT TGCAGAAATC CTTGTGGCCA CAATTGCTTT TCTGTTACCA 
AATGCAGAGT ACTCCTCGGT GGAGACAGAC AAGAAGTTCC TCGTGTCCTT GTTGCTCTGC 
CTCCTGGACT GGTGCATGGC GCTGCCGGCA GGAACCCTCC TCCACCCCGT GTCCACGGCG 
GCCCTGGACG AACAGGCCCC GGCCCGGGCC CCCTTACTGG ATTACATCTA CAGGGTTCTG 
CACTGCTGCA TCTGTGGCTC AAGCACGTAC AGCCAGCAGA GCCGCTACAC CTTGACCCTG 
GCGGACCTGT CGTCCTCGGA TTACGACCCC TTCCTGCCGC TGGCGAACGT GAAGAATTCT 
GAGCCGGTCC AATTTCATTC ATCAGCAGAA CTGGGCAACC TGCTCACTGT TGAAGAGGCA 
GAGAGGAGGA GAAGGAGCCT GGGGCTGATC CCGCTGACAG CGCGCATGGT GATGGCGCAC 
CTGGTCAACC ACCTGGGCCA CTTCCCGCTC TGCGGGGGCC CCGCCGTGCT GCACAGCCTG 
GTGGGCGAGC ACCACGACAA CGTGCACGCC GACGCCACCG AGCTCTCCCC AGACGTGTTC 
AGGAGCCCCA GCCTGCAGCT GTTTGTGTTC AACGACAGCA CCCTCATCTC CTACCTGCAG 
ACGCCTGCGA CCGGTCCTGC CAGTGGGTCG CCAGCGGGCG CCCCCTCGGA CGTGAGAGTG 
ATCGTGAGGG ACATCTCAGG GAAGTACTCG TGGGACGGGA AGGTGCTCTA CGGACCCCTG 
GAAGGCCACT TAGCCCCCAA GGGCAGAAGC CCCTCGTTCC TCATCTCGAG CTGTCAACCC 
CCAACCCCTG GACCTCAGAG GGCTCCTTCT CAGACTGAGG AGGGAGAGGA TGTCCTCGAC 
AAATTACTTG AAAACCTTGG CCACACGAGT CCTGAATGCC TTTTGCCATC CCAGCTCCGT 
CTGAATGAGC CTTCCCCACC CCCTTGTGGC ATGAATTGGG ACCAGGAGAA GGAGATCACC 
AAGGTCCTGC TGCGCCAGAG CACGCAAGAA GACGAGTATG TCCAGAGGTG CAACTCGGAT 
GTGGCCATGA GGGTCGCCAG CCAGGAGCCG CCGTCCCCAG TGGAGCCCCG GGGCGCCTTC 
TACTTCTGTA GGCTGCTGCT CGATGACTTG GGAATGAATT CTTGGGACAG AAGGAAGAAC 
TTCCATCTGT TGAAGAAAAA TTCAAAATTA TTGAGAGAGC TAAAAAATCT GGACTCCCGG 
CAGTGCCGTG AAACGCACAA AATCGCAGTG TTTTACATTG CCGAAGGTCA AGAAGACAAG 
TGTTCGATCC TTTCCAATGA AAGGGGAAGC CAAGCGTATG AAGACTTTGT TGCTGGACTT 
GGATGGGAGG TGGACCTGTC AACCCACTGT GGGTTCATGG GCGGGCTTCA GCGCAATGGC 
AGCACGGGCC AGACCGCCCC CTACTACGCT ACCTCGACCG TGGAAGTCAT CTTCCACGTC 
TCAACTCGGA TGCCGTCAGA CTCAGACGAC TCGCTCACCA AAAAGCTCCG TCACTTGGGG 
AATGACGAAG TCCACATCGT CTGGTCCGAG CACTCCAGGG ACTACCGCAG CGGGATCATC 
CCCACCGCCT TCGGAGACGT TTCAATCATC ATCTACCCCA TGAAGAATCA CATGTTCTTT 
ATCGCGATAA CCAAGAAACC CGAGGTTCCA TTTTTTGGGC CTCTGTTTGA TGGAGCAATC 
GTGAGTGGGA AGCTACTGCC AAGCCTTATC TGTGCCACCT GCATCAACGC CAGCCGGGCC 
GTCAAGTGCC TCATCCCCCT CTACCAGAGC TTCTACGAGG AGAGAGCTCT CTATCTGGAA 
GCAATAATTC AGAACCACCG TGAAGTCATG ACATTTGAGG ATTTCACAGC CCAGGTCTTC 
TGTCCATCTC CCAGCTACTC GGTCAGCGGA ACTGTGTCAG CTCTGGAAGA GGGAAGAGAT 
GGATGTGATG AACATCTGCT CCACCTGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465968.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321454.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.

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CloneID	OBa94787
Clone ID Related Accession (Same CDS sequence)	XM_015465968.1
Accession Version	XM_015465968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGTGTCA 
GCTCTGGAAG AGGGAAGAGA TGGATGTGAT GAACATCTGC TCCACCTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321454.1
CDS	375..5708
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015465968.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465968.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGTGTCA 
GCTCTGGAAG AGGGAAGAGA TGGATGTGAT GAACATCTGC TCCACCTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465969.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321455.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.

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CloneID	OBa94788
Clone ID Related Accession (Same CDS sequence)	XM_015465969.1
Accession Version	XM_015465969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGA GAGGAGGAGA AGGAGCCTGG GGCTGATCCC GCTGACAGCG 
CGCATGGTGA TGGCGCACCT GGTCAACCAC CTGGGCCACT TCCCGCTCTG CGGGGGCCCC 
GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC CACGACAACG TGCACGCCGA CGCCACCGAG 
CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC CTGCAGCTGT TTGTGTTCAA CGACAGCACC 
CTCATCTCCT ACCTGCAGAC GCCTGCGACC GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC 
CCCTCGGACG TGAGAGTGAT CGTGAGGGAC ATCTCAGGGA AGTACTCGTG GGACGGGAAG 
GTGCTCTACG GACCCCTGGA AGGCCACTTA GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC 
ATCTCGAGCT GTCAACCCCC AACCCCTGGA CCTCAGAGGG CTCCTTCTCA GACTGAGGAG 
GGAGAGGATG TCCTCGACAA ATTACTTGAA AACCTTGGCC ACACGAGTCC TGAATGCCTT 
TTGCCATCCC AGCTCCGTCT GAATGAGCCT TCCCCACCCC CTTGTGGCAT GAATTGGGAC 
CAGGAGAAGG AGATCACCAA GGTCCTGCTG CGCCAGAGCA CGCAAGAAGA CGAGTATGTC 
CAGAGGTGCA ACTCGGATGT GGCCATGAGG GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG 
GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG CTGCTGCTCG ATGACTTGGG AATGAATTCT 
TGGGACAGAA GGAAGAACTT CCATCTGTTG AAGAAAAATT CAAAATTATT GAGAGAGCTA 
AAAAATCTGG ACTCCCGGCA GTGCCGTGAA ACGCACAAAA TCGCAGTGTT TTACATTGCC 
GAAGGTCAAG AAGACAAGTG TTCGATCCTT TCCAATGAAA GGGGAAGCCA AGCGTATGAA 
GACTTTGTTG CTGGACTTGG ATGGGAGGTG GACCTGTCAA CCCACTGTGG GTTCATGGGC 
GGGCTTCAGC GCAATGGCAG CACGGGCCAG ACCGCCCCCT ACTACGCTAC CTCGACCGTG 
GAAGTCATCT TCCACGTCTC AACTCGGATG CCGTCAGACT CAGACGACTC GCTCACCAAA 
AAGCTCCGTC ACTTGGGGAA TGACGAAGTC CACATCGTCT GGTCCGAGCA CTCCAGGGAC 
TACCGCAGCG GGATCATCCC CACCGCCTTC GGAGACGTTT CAATCATCAT CTACCCCATG 
AAGAATCACA TGTTCTTTAT CGCGATAACC AAGAAACCCG AGGTTCCATT TTTTGGGCCT 
CTGTTTGATG GAGCAATCGT GAGTGGGAAG CTACTGCCAA GCCTTATCTG TGCCACCTGC 
ATCAACGCCA GCCGGGCCGT CAAGTGCCTC ATCCCCCTCT ACCAGAGCTT CTACGAGGAG 
AGAGCTCTCT ATCTGGAAGC AATAATTCAG AACCACCGTG AAGTCATGAC ATTTGAGGAT 
TTCACAGCCC AGGTCTTCTG TCCATCTCCC AGCTACTCGG TCAGCGGAAC TGTGTCAGCT 
CTGGAAGAGG GAAGAGATGG ATGTGATGAA CATCTGCTCC ACCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321455.1
CDS	375..5705
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_015465969.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465969.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGA GAGGAGGAGA AGGAGCCTGG GGCTGATCCC GCTGACAGCG 
CGCATGGTGA TGGCGCACCT GGTCAACCAC CTGGGCCACT TCCCGCTCTG CGGGGGCCCC 
GCCGTGCTGC ACAGCCTGGT GGGCGAGCAC CACGACAACG TGCACGCCGA CGCCACCGAG 
CTCTCCCCAG ACGTGTTCAG GAGCCCCAGC CTGCAGCTGT TTGTGTTCAA CGACAGCACC 
CTCATCTCCT ACCTGCAGAC GCCTGCGACC GGTCCTGCCA GTGGGTCGCC AGCGGGCGCC 
CCCTCGGACG TGAGAGTGAT CGTGAGGGAC ATCTCAGGGA AGTACTCGTG GGACGGGAAG 
GTGCTCTACG GACCCCTGGA AGGCCACTTA GCCCCCAAGG GCAGAAGCCC CTCGTTCCTC 
ATCTCGAGCT GTCAACCCCC AACCCCTGGA CCTCAGAGGG CTCCTTCTCA GACTGAGGAG 
GGAGAGGATG TCCTCGACAA ATTACTTGAA AACCTTGGCC ACACGAGTCC TGAATGCCTT 
TTGCCATCCC AGCTCCGTCT GAATGAGCCT TCCCCACCCC CTTGTGGCAT GAATTGGGAC 
CAGGAGAAGG AGATCACCAA GGTCCTGCTG CGCCAGAGCA CGCAAGAAGA CGAGTATGTC 
CAGAGGTGCA ACTCGGATGT GGCCATGAGG GTCGCCAGCC AGGAGCCGCC GTCCCCAGTG 
GAGCCCCGGG GCGCCTTCTA CTTCTGTAGG CTGCTGCTCG ATGACTTGGG AATGAATTCT 
TGGGACAGAA GGAAGAACTT CCATCTGTTG AAGAAAAATT CAAAATTATT GAGAGAGCTA 
AAAAATCTGG ACTCCCGGCA GTGCCGTGAA ACGCACAAAA TCGCAGTGTT TTACATTGCC 
GAAGGTCAAG AAGACAAGTG TTCGATCCTT TCCAATGAAA GGGGAAGCCA AGCGTATGAA 
GACTTTGTTG CTGGACTTGG ATGGGAGGTG GACCTGTCAA CCCACTGTGG GTTCATGGGC 
GGGCTTCAGC GCAATGGCAG CACGGGCCAG ACCGCCCCCT ACTACGCTAC CTCGACCGTG 
GAAGTCATCT TCCACGTCTC AACTCGGATG CCGTCAGACT CAGACGACTC GCTCACCAAA 
AAGCTCCGTC ACTTGGGGAA TGACGAAGTC CACATCGTCT GGTCCGAGCA CTCCAGGGAC 
TACCGCAGCG GGATCATCCC CACCGCCTTC GGAGACGTTT CAATCATCAT CTACCCCATG 
AAGAATCACA TGTTCTTTAT CGCGATAACC AAGAAACCCG AGGTTCCATT TTTTGGGCCT 
CTGTTTGATG GAGCAATCGT GAGTGGGAAG CTACTGCCAA GCCTTATCTG TGCCACCTGC 
ATCAACGCCA GCCGGGCCGT CAAGTGCCTC ATCCCCCTCT ACCAGAGCTT CTACGAGGAG 
AGAGCTCTCT ATCTGGAAGC AATAATTCAG AACCACCGTG AAGTCATGAC ATTTGAGGAT 
TTCACAGCCC AGGTCTTCTG TCCATCTCCC AGCTACTCGG TCAGCGGAAC TGTGTCAGCT 
CTGGAAGAGG GAAGAGATGG ATGTGATGAA CATCTGCTCC ACCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465971.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321457.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321457.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465971.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465971.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465974.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321460.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321460.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465974.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465974.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465970.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321456.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321456.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465970.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465970.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465972.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321458.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321458.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465972.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465972.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465975.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321461.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321461.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465975.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465975.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822472.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820774.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_010822472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820774.2
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_010822472.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822472.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465973.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321459.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321459.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465973.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465973.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822473.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820775.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_010822473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820775.2
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_010822473.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822473.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465976.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321462.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.

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CloneID	OBa94789
Clone ID Related Accession (Same CDS sequence)	XM_015465971.1 , XM_015465974.1 , XM_015465970.1 , XM_015465972.1 , XM_015465975.1 , XM_010822472.2 , XM_015465973.1 , XM_010822473.2 , XM_015465976.1
Accession Version	XM_015465976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321462.1
CDS	375..5693
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTGQVQSNPDEPGSLN

Target ORF information:

RefSeq Version	XM_015465976.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465976.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGGACAA 
GTACAGTCAA ACCCAGACGA GCCAGGCAGC TTAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465979.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321465.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.

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CloneID	OBa94790
Clone ID Related Accession (Same CDS sequence)	XM_015465979.1 , XM_015465978.1 , XM_015465980.1 , XM_015465977.1
Accession Version	XM_015465979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321465.1
CDS	375..5660
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015465979.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465979.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465978.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321464.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.

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CloneID	OBa94790
Clone ID Related Accession (Same CDS sequence)	XM_015465979.1 , XM_015465978.1 , XM_015465980.1 , XM_015465977.1
Accession Version	XM_015465978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321464.1
CDS	375..5660
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015465978.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465978.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465980.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321466.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.

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CloneID	OBa94790
Clone ID Related Accession (Same CDS sequence)	XM_015465979.1 , XM_015465978.1 , XM_015465980.1 , XM_015465977.1
Accession Version	XM_015465980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321466.1
CDS	375..5660
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015465980.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465980.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_015465977.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321463.1 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.

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CloneID	OBa94790
Clone ID Related Accession (Same CDS sequence)	XM_015465979.1 , XM_015465978.1 , XM_015465980.1 , XM_015465977.1
Accession Version	XM_015465977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321463.1
CDS	375..5660
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTELL

Target ORF information:

RefSeq Version	XM_015465977.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465977.1

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGAACTG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822475.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820777.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.

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CloneID	OBa94791
Clone ID Related Accession (Same CDS sequence)	XM_010822475.2 , XM_010822474.2 , XM_010822477.2 , XM_010822476.2
Accession Version	XM_010822475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820777.2
CDS	375..5654
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_010822475.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822475.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822474.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820776.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.

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CloneID	OBa94791
Clone ID Related Accession (Same CDS sequence)	XM_010822475.2 , XM_010822474.2 , XM_010822477.2 , XM_010822476.2
Accession Version	XM_010822474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820776.2
CDS	375..5654
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_010822474.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822474.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822477.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820779.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.

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CloneID	OBa94791
Clone ID Related Accession (Same CDS sequence)	XM_010822475.2 , XM_010822474.2 , XM_010822477.2 , XM_010822476.2
Accession Version	XM_010822477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820779.2
CDS	375..5654
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_010822477.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822477.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822476.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820778.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.

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CloneID	OBa94791
Clone ID Related Accession (Same CDS sequence)	XM_010822475.2 , XM_010822474.2 , XM_010822477.2 , XM_010822476.2
Accession Version	XM_010822476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820778.2
CDS	375..5654
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVPDSQKGSTVGRSFSLSWRSHPDVAVAEPMRFRSATTSGAPGVEKARNVVRQKATAKRSQSISNCAHLSEALPATKSVPLLLHTVSALFPGLSYSSCSHRLSEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTD

Target ORF information:

RefSeq Version	XM_010822476.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822476.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGGCTGT 
GTTCCAGATT CTCAGAAAGG AAGCACCGTG GGGAGGTCCT TTTCTCTGAG CTGGCGGAGC 
CACCCCGACG TGGCCGTGGC CGAGCCCATG AGATTCAGGA GCGCCACCAC GTCCGGGGCG 
CCAGGGGTCG AGAAAGCCAG AAACGTTGTC CGCCAGAAGG CAACCGCTAA GCGAAGCCAG 
TCTATCAGCA ACTGTGCCCA CCTCTCTGAG GCTTTGCCCG CTACTAAAAG CGTCCCACTC 
CTCCTCCACA CCGTGAGCGC TCTCTTCCCT GGTCTCTCTT ACTCTTCTTG CTCCCACAGG 
CTGTCAGAAG TGGAAGAATA CCTGCAGCCA GAAAACACAC CTGCCACCGA ATCTGGGCCC 
CTGGGGCAGC AGCCGATGGT TCGGAGCGGC AGCAGCCCTG AGGTCACCGA GCCGCTGTGT 
CCTGAGCCCC CCCCAGGCCA AAAGCTAGAA AATGCACAGA ACGTGAGTTC TTCAGAGCCC 
AGACTTGCTC AGGAGAGCAA AGGACATGAG AAGAAAGAGC ATGAAGGAAT AACAATCTTA 
GTTCGAAGAA GCAGCAGCCC CATTGAACTG GATTTGAAAG ATGACTCACA GCAGATGCAA 
GGAAAACGTA GGGAGAGACA GAAGAGTGAC AGTGTGAGCA GTGACCCCAC TCTGGGCTGC 
AGCACTGAGG CAGAGCTGTC CCTGAGCCAC TGGCAGACCT GCGAGGAAGA CCCAGATCTG 
AGCCCGCCTG CAGATACTGT GACCGACGCT GACGCCCGCC ACTGGTTACA GCTCCGGCCC 
ACGGATGCTT CAAACCTAAC AGACAGCAGC GAATGCCTTG CTGATGACTG CAGTATAATT 
GCTGGGGGGA CCCTCACTGG TTGGCACCCA GACTCAGCCG CTGTGTTGTG GCGAAGAATC 
TTGGGAATCC TTGGAGATGT GAATAATATC CAGTCACCCA AAATCCATGC CAGGGTGTTT 
GGCTATCTCT ATGAACTCTG GTACAAGCTA GCAAAGATAC GGGATAACCT GGCAATAAGC 
CTGGATAACC AGTCTTCTCC ATCTCCTCCT GCCTTGATCC CACCGCTCAG AATGTTTGCA 
TCATGGCTAT TTAAGGCGAC GACGATGCCT GACGATTATA AGGAAGGCAA ACTGCAAGCC 
TATAAGCTGA TCTGCTCCAT GATGACCCGG CGCCAGGACG TCCTGCCCAA CTCAGACTTC 
CTGGTGCATT TTTACCTCGT CATGCACCTG GGACTGACCA GCGAGGACCA GGATATCCTG 
AATACCATCA TAAGGCACTG CCCGCCCCGC TTCTTCTCCC TGGGCTTGCC TGGCTTCTCC 
ATGCTGGTGG GGGACTTCAT CGTGGCTGCT GGCAGGGTCC TCAGTGCAGA CACGCTGGCG 
GCACCTCGTT CGGAAGCCCT CACCATTCTC GGGTCTCTGG TCTGCTTTCC GAACACCTAC 
CGCGAGATTC CCCTACGGCA GCCGGTACCT GAAGTTGCTG AAGTCGCCAT GGGAGCAGAA 
GATGTCAAGC ACTGCCTCAT AAGTATCTTA CTGAAGAATG CCACAGAGGA ACCGAGTGAG 
TGTGCAAGGT GCATTGCCAT CTGCTCCCTC GGGATCTGGA TCTGTGAGGA GCTGGCACAA 
GGCACTGGCC ACCCTCAGCT GCAAGAGGCC GTCAACGTCA TTGGTGTCAC GTTGAAGTTT 
CCTAACAAAA TTGTGGCCCA GGTAGCCTGT GACGTGCTTC AGCTGCTAGT CTCCTACTGG 
GAAAAGCTTC AGATGTTTGA AACCTCCTTG CCTCGGAAAA TTGCAGAAAT CCTTGTGGCC 
ACAATTGCTT TTCTGTTACC AAATGCAGAG TACTCCTCGG TGGAGACAGA CAAGAAGTTC 
CTCGTGTCCT TGTTGCTCTG CCTCCTGGAC TGGTGCATGG CGCTGCCGGC AGGAACCCTC 
CTCCACCCCG TGTCCACGGC GGCCCTGGAC GAACAGGCCC CGGCCCGGGC CCCCTTACTG 
GATTACATCT ACAGGGTTCT GCACTGCTGC ATCTGTGGCT CAAGCACGTA CAGCCAGCAG 
AGCCGCTACA CCTTGACCCT GGCGGACCTG TCGTCCTCGG ATTACGACCC CTTCCTGCCG 
CTGGCGAACG TGAAGAATTC TGAGCCGGTC CAATTTCATT CATCAGCAGA ACTGGGCAAC 
CTGCTCACTG TTGAAGAGGC AGAGAGGAGG AGAAGGAGCC TGGGGCTGAT CCCGCTGACA 
GCGCGCATGG TGATGGCGCA CCTGGTCAAC CACCTGGGCC ACTTCCCGCT CTGCGGGGGC 
CCCGCCGTGC TGCACAGCCT GGTGGGCGAG CACCACGACA ACGTGCACGC CGACGCCACC 
GAGCTCTCCC CAGACGTGTT CAGGAGCCCC AGCCTGCAGC TGTTTGTGTT CAACGACAGC 
ACCCTCATCT CCTACCTGCA GACGCCTGCG ACCGGTCCTG CCAGTGGGTC GCCAGCGGGC 
GCCCCCTCGG ACGTGAGAGT GATCGTGAGG GACATCTCAG GGAAGTACTC GTGGGACGGG 
AAGGTGCTCT ACGGACCCCT GGAAGGCCAC TTAGCCCCCA AGGGCAGAAG CCCCTCGTTC 
CTCATCTCGA GCTGTCAACC CCCAACCCCT GGACCTCAGA GGGCTCCTTC TCAGACTGAG 
GAGGGAGAGG ATGTCCTCGA CAAATTACTT GAAAACCTTG GCCACACGAG TCCTGAATGC 
CTTTTGCCAT CCCAGCTCCG TCTGAATGAG CCTTCCCCAC CCCCTTGTGG CATGAATTGG 
GACCAGGAGA AGGAGATCAC CAAGGTCCTG CTGCGCCAGA GCACGCAAGA AGACGAGTAT 
GTCCAGAGGT GCAACTCGGA TGTGGCCATG AGGGTCGCCA GCCAGGAGCC GCCGTCCCCA 
GTGGAGCCCC GGGGCGCCTT CTACTTCTGT AGGCTGCTGC TCGATGACTT GGGAATGAAT 
TCTTGGGACA GAAGGAAGAA CTTCCATCTG TTGAAGAAAA ATTCAAAATT ATTGAGAGAG 
CTAAAAAATC TGGACTCCCG GCAGTGCCGT GAAACGCACA AAATCGCAGT GTTTTACATT 
GCCGAAGGTC AAGAAGACAA GTGTTCGATC CTTTCCAATG AAAGGGGAAG CCAAGCGTAT 
GAAGACTTTG TTGCTGGACT TGGATGGGAG GTGGACCTGT CAACCCACTG TGGGTTCATG 
GGCGGGCTTC AGCGCAATGG CAGCACGGGC CAGACCGCCC CCTACTACGC TACCTCGACC 
GTGGAAGTCA TCTTCCACGT CTCAACTCGG ATGCCGTCAG ACTCAGACGA CTCGCTCACC 
AAAAAGCTCC GTCACTTGGG GAATGACGAA GTCCACATCG TCTGGTCCGA GCACTCCAGG 
GACTACCGCA GCGGGATCAT CCCCACCGCC TTCGGAGACG TTTCAATCAT CATCTACCCC 
ATGAAGAATC ACATGTTCTT TATCGCGATA ACCAAGAAAC CCGAGGTTCC ATTTTTTGGG 
CCTCTGTTTG ATGGAGCAAT CGTGAGTGGG AAGCTACTGC CAAGCCTTAT CTGTGCCACC 
TGCATCAACG CCAGCCGGGC CGTCAAGTGC CTCATCCCCC TCTACCAGAG CTTCTACGAG 
GAGAGAGCTC TCTATCTGGA AGCAATAATT CAGAACCACC GTGAAGTCAT GACATTTGAG 
GATTTCACAG CCCAGGTCTT CTGTCCATCT CCCAGCTACT CGGTCAGCGG AACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RALGAPA2 ( XM_010822479.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820781.2 Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.

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CloneID	OBa94792
Clone ID Related Accession (Same CDS sequence)	XM_010822479.2
Accession Version	XM_010822479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ral GTPase-activating protein subunit alpha-2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646131.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGAAGTG 
GAAGAATACC TGCAGCCAGA AAACACACCT GCCACCGAAT CTGGGCCCCT GGGGCAGCAG 
CCGATGGTTC GGAGCGGCAG CAGCCCTGAG GTCACCGAGC CGCTGTGTCC TGAGCCCCCC 
CCAGGCCAAA AGCTAGAAAA TGCACAGAAC GTGAGTTCTT CAGAGCCCAG ACTTGCTCAG 
GAGAGCAAAG GACATGAGAA GAAAGAGCAT GAAGGAATAA CAATCTTAGT TCGAAGAAGC 
AGCAGCCCCA TTGAACTGGA TTTGAAAGAT GACTCACAGC AGATGCAAGG AAAACGTAGG 
GAGAGACAGA AGAGTGACAG TGTGAGCAGT GACCCCACTC TGGGCTGCAG CACTGAGGCA 
GAGCTGTCCC TGAGCCACTG GCAGACCTGC GAGGAAGACC CAGATCTGAG CCCGCCTGCA 
GATACTGTGA CCGACGCTGA CGCCCGCCAC TGGTTACAGC TCCGGCCCAC GGATGCTTCA 
AACCTAACAG ACAGCAGCGA ATGCCTTGCT GATGACTGCA GTATAATTGC TGGGGGGACC 
CTCACTGGTT GGCACCCAGA CTCAGCCGCT GTGTTGTGGC GAAGAATCTT GGGAATCCTT 
GGAGATGTGA ATAATATCCA GTCACCCAAA ATCCATGCCA GGGTGTTTGG CTATCTCTAT 
GAACTCTGGT ACAAGCTAGC AAAGATACGG GATAACCTGG CAATAAGCCT GGATAACCAG 
TCTTCTCCAT CTCCTCCTGC CTTGATCCCA CCGCTCAGAA TGTTTGCATC ATGGCTATTT 
AAGGCGACGA CGATGCCTGA CGATTATAAG GAAGGCAAAC TGCAAGCCTA TAAGCTGATC 
TGCTCCATGA TGACCCGGCG CCAGGACGTC CTGCCCAACT CAGACTTCCT GGTGCATTTT 
TACCTCGTCA TGCACCTGGG ACTGACCAGC GAGGACCAGG ATATCCTGAA TACCATCATA 
AGGCACTGCC CGCCCCGCTT CTTCTCCCTG GGCTTGCCTG GCTTCTCCAT GCTGGTGGGG 
GACTTCATCG TGGCTGCTGG CAGGGTCCTC AGTGCAGACA CGCTGGCGGC ACCTCGTTCG 
GAAGCCCTCA CCATTCTCGG GTCTCTGGTC TGCTTTCCGA ACACCTACCG CGAGATTCCC 
CTACGGCAGC CGGTACCTGA AGTTGCTGAA GTCGCCATGG GAGCAGAAGA TGTCAAGCAC 
TGCCTCATAA GTATCTTACT GAAGAATGCC ACAGAGGAAC CGAGTGAGTG TGCAAGGTGC 
ATTGCCATCT GCTCCCTCGG GATCTGGATC TGTGAGGAGC TGGCACAAGG CACTGGCCAC 
CCTCAGCTGC AAGAGGCCGT CAACGTCATT GGTGTCACGT TGAAGTTTCC TAACAAAATT 
GTGGCCCAGG TAGCCTGTGA CGTGCTTCAG CTGCTAGTCT CCTACTGGGA AAAGCTTCAG 
ATGTTTGAAA CCTCCTTGCC TCGGAAAATT GCAGAAATCC TTGTGGCCAC AATTGCTTTT 
CTGTTACCAA ATGCAGAGTA CTCCTCGGTG GAGACAGACA AGAAGTTCCT CGTGTCCTTG 
TTGCTCTGCC TCCTGGACTG GTGCATGGCG CTGCCGGCAG GAACCCTCCT CCACCCCGTG 
TCCACGGCGG CCCTGGACGA ACAGGCCCCG GCCCGGGCCC CCTTACTGGA TTACATCTAC 
AGGGTTCTGC ACTGCTGCAT CTGTGGCTCA AGCACGTACA GCCAGCAGAG CCGCTACACC 
TTGACCCTGG CGGACCTGTC GTCCTCGGAT TACGACCCCT TCCTGCCGCT GGCGAACGTG 
AAGAATTCTG AGCCGGTCCA ATTTCATTCA TCAGCAGAAC TGGGCAACCT GCTCACTGTT 
GAAGAGGCAG AGAGGAGGAG AAGGAGCCTG GGGCTGATCC CGCTGACAGC GCGCATGGTG 
ATGGCGCACC TGGTCAACCA CCTGGGCCAC TTCCCGCTCT GCGGGGGCCC CGCCGTGCTG 
CACAGCCTGG TGGGCGAGCA CCACGACAAC GTGCACGCCG ACGCCACCGA GCTCTCCCCA 
GACGTGTTCA GGAGCCCCAG CCTGCAGCTG TTTGTGTTCA ACGACAGCAC CCTCATCTCC 
TACCTGCAGA CGCCTGCGAC CGGTCCTGCC AGTGGGTCGC CAGCGGGCGC CCCCTCGGAC 
GTGAGAGTGA TCGTGAGGGA CATCTCAGGG AAGTACTCGT GGGACGGGAA GGTGCTCTAC 
GGACCCCTGG AAGGCCACTT AGCCCCCAAG GGCAGAAGCC CCTCGTTCCT CATCTCGAGC 
TGTCAACCCC CAACCCCTGG ACCTCAGAGG GCTCCTTCTC AGACTGAGGA GGGAGAGGAT 
GTCCTCGACA AATTACTTGA AAACCTTGGC CACACGAGTC CTGAATGCCT TTTGCCATCC 
CAGCTCCGTC TGAATGAGCC TTCCCCACCC CCTTGTGGCA TGAATTGGGA CCAGGAGAAG 
GAGATCACCA AGGTCCTGCT GCGCCAGAGC ACGCAAGAAG ACGAGTATGT CCAGAGGTGC 
AACTCGGATG TGGCCATGAG GGTCGCCAGC CAGGAGCCGC CGTCCCCAGT GGAGCCCCGG 
GGCGCCTTCT ACTTCTGTAG GCTGCTGCTC GATGACTTGG GAATGAATTC TTGGGACAGA 
AGGAAGAACT TCCATCTGTT GAAGAAAAAT TCAAAATTAT TGAGAGAGCT AAAAAATCTG 
GACTCCCGGC AGTGCCGTGA AACGCACAAA ATCGCAGTGT TTTACATTGC CGAAGGTCAA 
GAAGACAAGT GTTCGATCCT TTCCAATGAA AGGGGAAGCC AAGCGTATGA AGACTTTGTT 
GCTGGACTTG GATGGGAGGT GGACCTGTCA ACCCACTGTG GGTTCATGGG CGGGCTTCAG 
CGCAATGGCA GCACGGGCCA GACCGCCCCC TACTACGCTA CCTCGACCGT GGAAGTCATC 
TTCCACGTCT CAACTCGGAT GCCGTCAGAC TCAGACGACT CGCTCACCAA AAAGCTCCGT 
CACTTGGGGA ATGACGAAGT CCACATCGTC TGGTCCGAGC ACTCCAGGGA CTACCGCAGC 
GGGATCATCC CCACCGCCTT CGGAGACGTT TCAATCATCA TCTACCCCAT GAAGAATCAC 
ATGTTCTTTA TCGCGATAAC CAAGAAACCC GAGGTTCCAT TTTTTGGGCC TCTGTTTGAT 
GGAGCAATCG TGAGTGGGAA GCTACTGCCA AGCCTTATCT GTGCCACCTG CATCAACGCC 
AGCCGGGCCG TCAAGTGCCT CATCCCCCTC TACCAGAGCT TCTACGAGGA GAGAGCTCTC 
TATCTGGAAG CAATAATTCA GAACCACCGT GAAGTCATGA CATTTGAGGA TTTCACAGCC 
CAGGTCTTCT GTCCATCTCC CAGCTACTCG GTCAGCGGAA CTGTGTCAGC TCTGGAAGAG 
GGAAGAGATG GATGTGATGA ACATCTGCTC CACCTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820781.2
CDS	375..5396
Translation	MSSRGPCTLDTLINPGPNMADAKIHPEEITPLLPAVSGEKIAEDQTCFFLQVLLKYMVTQAASLEWKHKENQETGFKFLFTLFRKYYLPHLFPSFTKSTNIYKPVLDIPHLRPKPVYVTTTRDNESIYSTKIPYMAARVVFIKWMVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGTAVPERVPEPDAGGAAEQDRGLTDGSTLCERRLSGCSLCGIEEEHRAVYDMVQRILLSTRGYVNFVNEVFRQAFLLPSCDIAVTRKVVQVYRKWILQDKPVFMEEPDIKEAAQEDAEKLGFSETDNKQTSSEGSSHKRSSSWGRSYSFTSAMSRGCVMEEENKNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLKEQVDACKAVLIIFRRMIMELTMNKTTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLIVAWIRANLCVYVSRELWDDFLGVLSSLTDWEELIHEWASIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKEVEEYLQPENTPATESGPLGQQPMVRSGSSPEVTEPLCPEPPPGQKLENAQNVSSSEPRLAQESKGHEKKEHEGITILVRRSSSPIELDLKDDSQQMQGKRRERQKSDSVSSDPTLGCSTEAELSLSHWQTCEEDPDLSPPADTVTDADARHWLQLRPTDASNLTDSSECLADDCSIIAGGTLTGWHPDSAAVLWRRILGILGDVNNIQSPKIHARVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPALIPPLRMFASWLFKATTMPDDYKEGKLQAYKLICSMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGLPGFSMLVGDFIVAAGRVLSADTLAAPRSEALTILGSLVCFPNTYREIPLRQPVPEVAEVAMGAEDVKHCLISILLKNATEEPSECARCIAICSLGIWICEELAQGTGHPQLQEAVNVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKIAEILVATIAFLLPNAEYSSVETDKKFLVSLLLCLLDWCMALPAGTLLHPVSTAALDEQAPARAPLLDYIYRVLHCCICGSSTYSQQSRYTLTLADLSSSDYDPFLPLANVKNSEPVQFHSSAELGNLLTVEEAERRRRSLGLIPLTARMVMAHLVNHLGHFPLCGGPAVLHSLVGEHHDNVHADATELSPDVFRSPSLQLFVFNDSTLISYLQTPATGPASGSPAGAPSDVRVIVRDISGKYSWDGKVLYGPLEGHLAPKGRSPSFLISSCQPPTPGPQRAPSQTEEGEDVLDKLLENLGHTSPECLLPSQLRLNEPSPPPCGMNWDQEKEITKVLLRQSTQEDEYVQRCNSDVAMRVASQEPPSPVEPRGAFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRSGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFTAQVFCPSPSYSVSGTVSALEEGRDGCDEHLLHLI

Target ORF information:

RefSeq Version	XM_010822479.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Ral GTPase activating protein, alpha subunit 2 (catalytic) (RALGAPA2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822479.2

ORF Insert Sequence:

ATGTCGTCCA GGGGGCCCTG TACACTGGAC ACGCTCATCA ACCCTGGCCC CAACATGGCT 
GATGCGAAGA TCCATCCGGA AGAAATCACT CCACTGCTGC CAGCCGTATC AGGGGAGAAG 
ATCGCCGAGG ACCAAACGTG CTTTTTTCTT CAAGTGCTGT TGAAATACAT GGTTACTCAG 
GCCGCAAGCT TGGAGTGGAA GCATAAGGAG AATCAGGAGA CTGGGTTTAA ATTTCTGTTT 
ACGTTGTTTC GAAAGTATTA CCTTCCTCAT CTGTTTCCCT CGTTTACCAA GTCAACCAAC 
ATCTACAAGC CTGTGCTGGA CATCCCCCAC CTGAGACCAA AGCCTGTGTA TGTCACTACC 
ACTCGAGACA ATGAGAGCAT TTACAGCACA AAGATCCCAT ACATGGCAGC TCGTGTCGTC 
TTCATTAAGT GGATGGTGAC TTTCTTTTTG GAAAAAAAGT ATCTAACTGC AACACAGAAC 
ACTAAGAATG GAGTTGATGT ATTGCCTAAA ATCATTCAGA CCGTCGGGGG AGGCACTGCA 
GTGCCCGAGC GGGTGCCGGA GCCAGACGCT GGCGGGGCGG CAGAGCAGGA CCGAGGCCTC 
ACGGATGGGA GCACCCTGTG CGAGCGCCGG CTCAGCGGCT GCAGCCTGTG CGGCATTGAG 
GAGGAGCACC GTGCGGTTTA CGACATGGTC CAGCGCATCC TGCTGTCCAC CCGCGGCTAT 
GTCAACTTCG TGAACGAGGT GTTCCGCCAG GCATTTTTGT TGCCTTCGTG CGACATAGCC 
GTAACCAGAA AAGTAGTTCA AGTGTACAGA AAGTGGATCC TGCAGGATAA ACCTGTGTTC 
ATGGAGGAAC CAGACATAAA AGAAGCTGCC CAAGAAGATG CTGAAAAATT AGGATTTTCA 
GAGACTGATA ACAAGCAGAC CTCTTCTGAA GGTTCCAGTC ACAAGCGGTC TTCCAGTTGG 
GGACGGTCTT ACTCCTTCAC CAGTGCCATG AGCAGAGGGT GTGTGATGGA AGAGGAGAAT 
AAGAACGTGA AGGCTGGGGC CCAGGCAATG CTGCAGGTAT TTCTGACAAA TGCTGCAAAC 
GTCTTTTTGC TGGAGCCTTG TGTGGAGGTT CCCATGCTCT TGAAGGAGCA AGTTGATGCC 
TGCAAAGCTG TTCTGATTAT CTTTAGGCGC ATGATAATGG AGCTGACAAT GAATAAAACG 
ACCTGGGAAC AGATGTTGCA AATACTGCTC AGGATAACAG AAGCTGTCAT GCAGAAGCCA 
AAGGATAAAC AAATCAAGGA CTTGTTTGCC CAGAGCTTGG CAGGGTTACT GTTTAGGACG 
CTCATAGTGG CCTGGATCCG AGCAAACCTC TGTGTGTACG TCTCCCGGGA GCTCTGGGAC 
GACTTTCTCG GGGTGCTCTC CTCCCTCACG GACTGGGAGG AGCTGATCCA CGAATGGGCC 
AGCATCATGG ACTCCTTGAC GGCTGTGCTG GCCAGGACCG TGTATGGAGT TGAGATGACC 
AATCTACCTC TGGACAAGTT AAGTGAACAG AAGGAAAAGA AACAGCGAGG CAAAGAAGTG 
GAAGAATACC TGCAGCCAGA AAACACACCT GCCACCGAAT CTGGGCCCCT GGGGCAGCAG 
CCGATGGTTC GGAGCGGCAG CAGCCCTGAG GTCACCGAGC CGCTGTGTCC TGAGCCCCCC 
CCAGGCCAAA AGCTAGAAAA TGCACAGAAC GTGAGTTCTT CAGAGCCCAG ACTTGCTCAG 
GAGAGCAAAG GACATGAGAA GAAAGAGCAT GAAGGAATAA CAATCTTAGT TCGAAGAAGC 
AGCAGCCCCA TTGAACTGGA TTTGAAAGAT GACTCACAGC AGATGCAAGG AAAACGTAGG 
GAGAGACAGA AGAGTGACAG TGTGAGCAGT GACCCCACTC TGGGCTGCAG CACTGAGGCA 
GAGCTGTCCC TGAGCCACTG GCAGACCTGC GAGGAAGACC CAGATCTGAG CCCGCCTGCA 
GATACTGTGA CCGACGCTGA CGCCCGCCAC TGGTTACAGC TCCGGCCCAC GGATGCTTCA 
AACCTAACAG ACAGCAGCGA ATGCCTTGCT GATGACTGCA GTATAATTGC TGGGGGGACC 
CTCACTGGTT GGCACCCAGA CTCAGCCGCT GTGTTGTGGC GAAGAATCTT GGGAATCCTT 
GGAGATGTGA ATAATATCCA GTCACCCAAA ATCCATGCCA GGGTGTTTGG CTATCTCTAT 
GAACTCTGGT ACAAGCTAGC AAAGATACGG GATAACCTGG CAATAAGCCT GGATAACCAG 
TCTTCTCCAT CTCCTCCTGC CTTGATCCCA CCGCTCAGAA TGTTTGCATC ATGGCTATTT 
AAGGCGACGA CGATGCCTGA CGATTATAAG GAAGGCAAAC TGCAAGCCTA TAAGCTGATC 
TGCTCCATGA TGACCCGGCG CCAGGACGTC CTGCCCAACT CAGACTTCCT GGTGCATTTT 
TACCTCGTCA TGCACCTGGG ACTGACCAGC GAGGACCAGG ATATCCTGAA TACCATCATA 
AGGCACTGCC CGCCCCGCTT CTTCTCCCTG GGCTTGCCTG GCTTCTCCAT GCTGGTGGGG 
GACTTCATCG TGGCTGCTGG CAGGGTCCTC AGTGCAGACA CGCTGGCGGC ACCTCGTTCG 
GAAGCCCTCA CCATTCTCGG GTCTCTGGTC TGCTTTCCGA ACACCTACCG CGAGATTCCC 
CTACGGCAGC CGGTACCTGA AGTTGCTGAA GTCGCCATGG GAGCAGAAGA TGTCAAGCAC 
TGCCTCATAA GTATCTTACT GAAGAATGCC ACAGAGGAAC CGAGTGAGTG TGCAAGGTGC 
ATTGCCATCT GCTCCCTCGG GATCTGGATC TGTGAGGAGC TGGCACAAGG CACTGGCCAC 
CCTCAGCTGC AAGAGGCCGT CAACGTCATT GGTGTCACGT TGAAGTTTCC TAACAAAATT 
GTGGCCCAGG TAGCCTGTGA CGTGCTTCAG CTGCTAGTCT CCTACTGGGA AAAGCTTCAG 
ATGTTTGAAA CCTCCTTGCC TCGGAAAATT GCAGAAATCC TTGTGGCCAC AATTGCTTTT 
CTGTTACCAA ATGCAGAGTA CTCCTCGGTG GAGACAGACA AGAAGTTCCT CGTGTCCTTG 
TTGCTCTGCC TCCTGGACTG GTGCATGGCG CTGCCGGCAG GAACCCTCCT CCACCCCGTG 
TCCACGGCGG CCCTGGACGA ACAGGCCCCG GCCCGGGCCC CCTTACTGGA TTACATCTAC 
AGGGTTCTGC ACTGCTGCAT CTGTGGCTCA AGCACGTACA GCCAGCAGAG CCGCTACACC 
TTGACCCTGG CGGACCTGTC GTCCTCGGAT TACGACCCCT TCCTGCCGCT GGCGAACGTG 
AAGAATTCTG AGCCGGTCCA ATTTCATTCA TCAGCAGAAC TGGGCAACCT GCTCACTGTT 
GAAGAGGCAG AGAGGAGGAG AAGGAGCCTG GGGCTGATCC CGCTGACAGC GCGCATGGTG 
ATGGCGCACC TGGTCAACCA CCTGGGCCAC TTCCCGCTCT GCGGGGGCCC CGCCGTGCTG 
CACAGCCTGG TGGGCGAGCA CCACGACAAC GTGCACGCCG ACGCCACCGA GCTCTCCCCA 
GACGTGTTCA GGAGCCCCAG CCTGCAGCTG TTTGTGTTCA ACGACAGCAC CCTCATCTCC 
TACCTGCAGA CGCCTGCGAC CGGTCCTGCC AGTGGGTCGC CAGCGGGCGC CCCCTCGGAC 
GTGAGAGTGA TCGTGAGGGA CATCTCAGGG AAGTACTCGT GGGACGGGAA GGTGCTCTAC 
GGACCCCTGG AAGGCCACTT AGCCCCCAAG GGCAGAAGCC CCTCGTTCCT CATCTCGAGC 
TGTCAACCCC CAACCCCTGG ACCTCAGAGG GCTCCTTCTC AGACTGAGGA GGGAGAGGAT 
GTCCTCGACA AATTACTTGA AAACCTTGGC CACACGAGTC CTGAATGCCT TTTGCCATCC 
CAGCTCCGTC TGAATGAGCC TTCCCCACCC CCTTGTGGCA TGAATTGGGA CCAGGAGAAG 
GAGATCACCA AGGTCCTGCT GCGCCAGAGC ACGCAAGAAG ACGAGTATGT CCAGAGGTGC 
AACTCGGATG TGGCCATGAG GGTCGCCAGC CAGGAGCCGC CGTCCCCAGT GGAGCCCCGG 
GGCGCCTTCT ACTTCTGTAG GCTGCTGCTC GATGACTTGG GAATGAATTC TTGGGACAGA 
AGGAAGAACT TCCATCTGTT GAAGAAAAAT TCAAAATTAT TGAGAGAGCT AAAAAATCTG 
GACTCCCGGC AGTGCCGTGA AACGCACAAA ATCGCAGTGT TTTACATTGC CGAAGGTCAA 
GAAGACAAGT GTTCGATCCT TTCCAATGAA AGGGGAAGCC AAGCGTATGA AGACTTTGTT 
GCTGGACTTG GATGGGAGGT GGACCTGTCA ACCCACTGTG GGTTCATGGG CGGGCTTCAG 
CGCAATGGCA GCACGGGCCA GACCGCCCCC TACTACGCTA CCTCGACCGT GGAAGTCATC 
TTCCACGTCT CAACTCGGAT GCCGTCAGAC TCAGACGACT CGCTCACCAA AAAGCTCCGT 
CACTTGGGGA ATGACGAAGT CCACATCGTC TGGTCCGAGC ACTCCAGGGA CTACCGCAGC 
GGGATCATCC CCACCGCCTT CGGAGACGTT TCAATCATCA TCTACCCCAT GAAGAATCAC 
ATGTTCTTTA TCGCGATAAC CAAGAAACCC GAGGTTCCAT TTTTTGGGCC TCTGTTTGAT 
GGAGCAATCG TGAGTGGGAA GCTACTGCCA AGCCTTATCT GTGCCACCTG CATCAACGCC 
AGCCGGGCCG TCAAGTGCCT CATCCCCCTC TACCAGAGCT TCTACGAGGA GAGAGCTCTC 
TATCTGGAAG CAATAATTCA GAACCACCGT GAAGTCATGA CATTTGAGGA TTTCACAGCC 
CAGGTCTTCT GTCCATCTCC CAGCTACTCG GTCAGCGGAA CTGTGTCAGC TCTGGAAGAG 
GGAAGAGATG GATGTGATGA ACATCTGCTC CACCTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

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オンライン注文

RALGAPA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

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