COL16A1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005203054.2	XP_005203111.1	collagen alpha-1(XVI) chain isoform X1
XM_005203055.2	XP_005203112.1	collagen alpha-1(XVI) chain isoform X2
XM_005203056.2	XP_005203113.1	collagen alpha-1(XVI) chain isoform X3
XM_015462427.1	XP_015317913.1	collagen alpha-1(XVI) chain isoform X2
XM_005203053.3	XP_005203110.2	collagen alpha-1(XVI) chain isoform X1
XM_005203054.3	XP_005203111.2	collagen alpha-1(XVI) chain isoform X1
XM_024982333.1	XP_024838101.1	collagen alpha-1(XVI) chain isoform X5
XM_010802506.2	XP_010800808.2	collagen alpha-1(XVI) chain isoform X7
XM_015462426.1	XP_015317912.1	collagen alpha-1(XVI) chain isoform X1
XM_005203053.2	XP_005203110.1	collagen alpha-1(XVI) chain isoform X1
XM_005203057.3	XP_005203114.2	collagen alpha-1(XVI) chain isoform X4
XM_015459964.2	XP_015315450.2	collagen alpha-1(XVI) chain isoform X6
XM_005203057.2	XP_005203114.1	collagen alpha-1(XVI) chain isoform X4
XM_015462428.1	XP_015317914.1	collagen alpha-1(XVI) chain isoform X3
XM_015462430.1	XP_015317916.1	collagen alpha-1(XVI) chain isoform X5
XM_010802506.1	XP_010800808.1	collagen alpha-1(XVI) chain isoform X6
XM_015462425.1	XP_015317911.1	collagen alpha-1(XVI) chain isoform X1
XM_015462429.1	XP_015317915.1	collagen alpha-1(XVI) chain isoform X4
XM_015459964.1	XP_015315450.1	collagen alpha-1(XVI) chain isoform X5
XM_015462431.1	XP_015317917.1	collagen alpha-1(XVI) chain isoform X6
XM_005203055.3	XP_005203112.2	collagen alpha-1(XVI) chain isoform X2
XM_005203056.3	XP_005203113.2	collagen alpha-1(XVI) chain isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227870	XM_005203053.3 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227870	XM_005203054.3 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227871	XM_005203055.3 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227872	XM_005203056.3 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227873	XM_005203057.3 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227874	XM_024982333.1 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227875	XM_015459964.2 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa227876	XM_010802506.2 Latest version!	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa16873	XM_015462425.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16873	XM_005203053.2 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16873	XM_015462426.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16873	XM_005203054.2 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16874	XM_015462427.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16874	XM_005203055.2 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16875	XM_005203056.2 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16875	XM_015462428.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16876	XM_005203057.2 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa16876	XM_015462429.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa46726	XM_010802506.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa46726	XM_015462431.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88038	XM_015462430.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88038	XM_015459964.1 Latest version!	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

COL16A1 ( XM_005203053.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203110.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa227870
Clone ID Related Accession (Same CDS sequence)	XM_005203053.3 , XM_005203054.3
Accession Version	XM_005203053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGAGAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203110.2
CDS	247..5145
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203053.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203053.3

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGAGAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203054.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203111.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X2, mRNA.

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CloneID	OBa227870
Clone ID Related Accession (Same CDS sequence)	XM_005203053.3 , XM_005203054.3
Accession Version	XM_005203054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGAGAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203111.2
CDS	382..5280
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203054.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203054.3

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGAGAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203055.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203112.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa227871
Clone ID Related Accession (Same CDS sequence)	XM_005203055.3
Accession Version	XM_005203055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGCCCCC CAGGTTTTCC TGGTGCTGTT 
GGCTCCCCCG GATTGCCTGG TCTTCAAGGA GAGCGAGGCC TCAGGGGCCT GACGGGAGAG 
AAGGGGGAGC CGGGTCCTCC AGGGCAACCT GGTTACCCAG GCGCCATGGG CCCCCCAGGA 
CTGCCTGGCA TCAAGGGGGA GCGAGGCTAC GCGGGTTCCC CGGGAGAGAA GGGAGAATCG 
GGCCCACCAG GATCTGAAGG CCTCCCAGGG CCCCCAGGCC CAGCGGGTCC CCGAGGAGAG 
CGAGGGCCCC AAGGAAGCTC GGGTGAGAAA GGAGACCAGG GATTCCAAGG GCAACCCGGC 
TTTCCAGGCC CCCCGGGTCC CCCTGGATTC CCAGGCAAAG CTGGAGCACC TGGCCCACCC 
GGCCCCCAAG CGGAGAAGGG CAGTGAAGGG ATTCGAGGCC CATCAGGTCT GCCTGGTCCC 
CCTGGACCAC CAGGATCTCC CGGGATTCAG GGCCCCGCCG GTCTGGATGG TCTGGATGGG 
AAGGATGGCA AACCTGGCTT GAGGGGGGAC CCTGGTCCTG CTGGCCCTCC CGGACTCATG 
GGACCCCCGG GCTTCAAGGG AAAACCCGGC CATCCTGGCC TCCCAGGACA GAAGGGCGAC 
TGTGGAAAGC CAGGCCCCCA GGGCAGCAGT GGCCGGCCCG GAGCAGAGGG TGAGCCTGGT 
GCCATGGGAC CCCAGGGAAG ACCCGGCCCC CCTGGCCATG TTGGGCCGCC GGGGCCTCCA 
GGCCAGCCAG GCCCAGCTGG GATCTCTGCA GTGGGCCCAA AAGGAGACCG TGGAGCCACT 
GGAGAAAGGG GCCTTTTAGG TCTTCCAGGC CAACCTGGCC CCCCTGGACA CCCTGGTCCC 
CCGGGTGAAC CCGGCATGGA TGGCGCAGCT GGCAAAGAGG GACCCCCTGG AAAACAGGGA 
CTCTATGGAC CCCCAGGTCC AAAGGGTGAC CCGGGGCCTT CAGGACAGAA GGGCCAGGCA 
GGAGAGAAGG GAAGAGCTGG CATGCCTGGC GGGCCCGGCA AGAGTGGCTC CATGGGACCT 
GCGGGGCCAC CGGGTCCTGC AGGAGAGAGA GGCCACCCTG GATCTCCGGG GCCTGCGGGG 
AGCCCTGGAT TGCCCGGGGT GCCCGGCTCC ATGGGAGACA TGGTGAATTA TGATGAAATC 
AAGAGGTTCA TCAGACAAGA GATCATTAAA ATGTTTGATG AGAGGATGGC TTACTACACC 
TCCAGGATGC AGTTTCCCAT GGAGATGGCA GCAGCTCCAG GGCGACCAGG GCCCCCAGGG 
AAGGATGGTC CCCCAGGCCG GCCAGGCTCC CCGGGATCAC CGGGACTCCC CGGTCAGATT 
GGCAGAGAAG GACGGCAGGG CTTGCCCGGG ATGAGAGGGT TGCCTGGTAC CAAAGGTGAA 
AAGGGAGACA TCGGTGTTGG CATCGCAGGA GAAATTGGTC TTCCTGGCCC CCCAGGTCCC 
CAGGGTCCTC CAGGGTACGG CAAGATGGGT GCGACTGGGC CGATGGGCCA GCAAGGCATT 
CCTGGCATCC CCGGTCCCCC AGGCCCCATA GGCCCGCCGG GCAAGACCGG CCACTGTAAC 
CCCTCTGACT GCTTTGGGGC CATGCCGATG GAGCAACAGT ACCCGCCCAT GAAAAACATG 
AAGGGACCTT TCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203112.2
CDS	247..5124
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203055.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203055.3

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGCCCCC CAGGTTTTCC TGGTGCTGTT 
GGCTCCCCCG GATTGCCTGG TCTTCAAGGA GAGCGAGGCC TCAGGGGCCT GACGGGAGAG 
AAGGGGGAGC CGGGTCCTCC AGGGCAACCT GGTTACCCAG GCGCCATGGG CCCCCCAGGA 
CTGCCTGGCA TCAAGGGGGA GCGAGGCTAC GCGGGTTCCC CGGGAGAGAA GGGAGAATCG 
GGCCCACCAG GATCTGAAGG CCTCCCAGGG CCCCCAGGCC CAGCGGGTCC CCGAGGAGAG 
CGAGGGCCCC AAGGAAGCTC GGGTGAGAAA GGAGACCAGG GATTCCAAGG GCAACCCGGC 
TTTCCAGGCC CCCCGGGTCC CCCTGGATTC CCAGGCAAAG CTGGAGCACC TGGCCCACCC 
GGCCCCCAAG CGGAGAAGGG CAGTGAAGGG ATTCGAGGCC CATCAGGTCT GCCTGGTCCC 
CCTGGACCAC CAGGATCTCC CGGGATTCAG GGCCCCGCCG GTCTGGATGG TCTGGATGGG 
AAGGATGGCA AACCTGGCTT GAGGGGGGAC CCTGGTCCTG CTGGCCCTCC CGGACTCATG 
GGACCCCCGG GCTTCAAGGG AAAACCCGGC CATCCTGGCC TCCCAGGACA GAAGGGCGAC 
TGTGGAAAGC CAGGCCCCCA GGGCAGCAGT GGCCGGCCCG GAGCAGAGGG TGAGCCTGGT 
GCCATGGGAC CCCAGGGAAG ACCCGGCCCC CCTGGCCATG TTGGGCCGCC GGGGCCTCCA 
GGCCAGCCAG GCCCAGCTGG GATCTCTGCA GTGGGCCCAA AAGGAGACCG TGGAGCCACT 
GGAGAAAGGG GCCTTTTAGG TCTTCCAGGC CAACCTGGCC CCCCTGGACA CCCTGGTCCC 
CCGGGTGAAC CCGGCATGGA TGGCGCAGCT GGCAAAGAGG GACCCCCTGG AAAACAGGGA 
CTCTATGGAC CCCCAGGTCC AAAGGGTGAC CCGGGGCCTT CAGGACAGAA GGGCCAGGCA 
GGAGAGAAGG GAAGAGCTGG CATGCCTGGC GGGCCCGGCA AGAGTGGCTC CATGGGACCT 
GCGGGGCCAC CGGGTCCTGC AGGAGAGAGA GGCCACCCTG GATCTCCGGG GCCTGCGGGG 
AGCCCTGGAT TGCCCGGGGT GCCCGGCTCC ATGGGAGACA TGGTGAATTA TGATGAAATC 
AAGAGGTTCA TCAGACAAGA GATCATTAAA ATGTTTGATG AGAGGATGGC TTACTACACC 
TCCAGGATGC AGTTTCCCAT GGAGATGGCA GCAGCTCCAG GGCGACCAGG GCCCCCAGGG 
AAGGATGGTC CCCCAGGCCG GCCAGGCTCC CCGGGATCAC CGGGACTCCC CGGTCAGATT 
GGCAGAGAAG GACGGCAGGG CTTGCCCGGG ATGAGAGGGT TGCCTGGTAC CAAAGGTGAA 
AAGGGAGACA TCGGTGTTGG CATCGCAGGA GAAATTGGTC TTCCTGGCCC CCCAGGTCCC 
CAGGGTCCTC CAGGGTACGG CAAGATGGGT GCGACTGGGC CGATGGGCCA GCAAGGCATT 
CCTGGCATCC CCGGTCCCCC AGGCCCCATA GGCCCGCCGG GCAAGACCGG CCACTGTAAC 
CCCTCTGACT GCTTTGGGGC CATGCCGATG GAGCAACAGT ACCCGCCCAT GAAAAACATG 
AAGGGACCTT TCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203056.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203113.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X4, mRNA.

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CloneID	OBa227872
Clone ID Related Accession (Same CDS sequence)	XM_005203056.3
Accession Version	XM_005203056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGTCTT 
CAAGGAGAGC GAGGCCTCAG GGGCCTGACG GGAGAGAAGG GGGAGCCGGG TCCTCCAGGG 
CAACCTGGTT ACCCAGGCGC CATGGGCCCC CCAGGACTGC CTGGCATCAA GGGGGAGCGA 
GGCTACGCGG GTTCCCCGGG AGAGAAGGGA GAATCGGGCC CACCAGGATC TGAAGGCCTC 
CCAGGGCCCC CAGGCCCAGC GGGTCCCCGA GGAGAGCGAG GGCCCCAAGG AAGCTCGGGT 
GAGAAAGGAG ACCAGGGATT CCAAGGGCAA CCCGGCTTTC CAGGCCCCCC GGGTCCCCCT 
GGATTCCCAG GCAAAGCTGG AGCACCTGGC CCACCCGGCC CCCAAGCGGA GAAGGGCAGT 
GAAGGGATTC GAGGCCCATC AGGTCTGCCT GGTCCCCCTG GACCACCAGG ATCTCCCGGG 
ATTCAGGGCC CCGCCGGTCT GGATGGTCTG GATGGGAAGG ATGGCAAACC TGGCTTGAGG 
GGGGACCCTG GTCCTGCTGG CCCTCCCGGA CTCATGGGAC CCCCGGGCTT CAAGGGAAAA 
CCCGGCCATC CTGGCCTCCC AGGACAGAAG GGCGACTGTG GAAAGCCAGG CCCCCAGGGC 
AGCAGTGGCC GGCCCGGAGC AGAGGGTGAG CCTGGTGCCA TGGGACCCCA GGGAAGACCC 
GGCCCCCCTG GCCATGTTGG GCCGCCGGGG CCTCCAGGCC AGCCAGGCCC AGCTGGGATC 
TCTGCAGTGG GCCCAAAAGG AGACCGTGGA GCCACTGGAG AAAGGGGCCT TTTAGGTCTT 
CCAGGCCAAC CTGGCCCCCC TGGACACCCT GGTCCCCCGG GTGAACCCGG CATGGATGGC 
GCAGCTGGCA AAGAGGGACC CCCTGGAAAA CAGGGACTCT ATGGACCCCC AGGTCCAAAG 
GGTGACCCGG GGCCTTCAGG ACAGAAGGGC CAGGCAGGAG AGAAGGGAAG AGCTGGCATG 
CCTGGCGGGC CCGGCAAGAG TGGCTCCATG GGACCTGCGG GGCCACCGGG TCCTGCAGGA 
GAGAGAGGCC ACCCTGGATC TCCGGGGCCT GCGGGGAGCC CTGGATTGCC CGGGGTGCCC 
GGCTCCATGG GAGACATGGT GAATTATGAT GAAATCAAGA GGTTCATCAG ACAAGAGATC 
ATTAAAATGT TTGATGAGAG GATGGCTTAC TACACCTCCA GGATGCAGTT TCCCATGGAG 
ATGGCAGCAG CTCCAGGGCG ACCAGGGCCC CCAGGGAAGG ATGGTCCCCC AGGCCGGCCA 
GGCTCCCCGG GATCACCGGG ACTCCCCGGT CAGATTGGCA GAGAAGGACG GCAGGGCTTG 
CCCGGGATGA GAGGGTTGCC TGGTACCAAA GGTGAAAAGG GAGACATCGG TGTTGGCATC 
GCAGGAGAAA TTGGTCTTCC TGGCCCCCCA GGTCCCCAGG GTCCTCCAGG GTACGGCAAG 
ATGGGTGCGA CTGGGCCGAT GGGCCAGCAA GGCATTCCTG GCATCCCCGG TCCCCCAGGC 
CCCATAGGCC CGCCGGGCAA GACCGGCCAC TGTAACCCCT CTGACTGCTT TGGGGCCATG 
CCGATGGAGC AACAGTACCC GCCCATGAAA AACATGAAGG GACCTTTCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203113.2
CDS	247..5100
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203056.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203056.3

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGTCTT 
CAAGGAGAGC GAGGCCTCAG GGGCCTGACG GGAGAGAAGG GGGAGCCGGG TCCTCCAGGG 
CAACCTGGTT ACCCAGGCGC CATGGGCCCC CCAGGACTGC CTGGCATCAA GGGGGAGCGA 
GGCTACGCGG GTTCCCCGGG AGAGAAGGGA GAATCGGGCC CACCAGGATC TGAAGGCCTC 
CCAGGGCCCC CAGGCCCAGC GGGTCCCCGA GGAGAGCGAG GGCCCCAAGG AAGCTCGGGT 
GAGAAAGGAG ACCAGGGATT CCAAGGGCAA CCCGGCTTTC CAGGCCCCCC GGGTCCCCCT 
GGATTCCCAG GCAAAGCTGG AGCACCTGGC CCACCCGGCC CCCAAGCGGA GAAGGGCAGT 
GAAGGGATTC GAGGCCCATC AGGTCTGCCT GGTCCCCCTG GACCACCAGG ATCTCCCGGG 
ATTCAGGGCC CCGCCGGTCT GGATGGTCTG GATGGGAAGG ATGGCAAACC TGGCTTGAGG 
GGGGACCCTG GTCCTGCTGG CCCTCCCGGA CTCATGGGAC CCCCGGGCTT CAAGGGAAAA 
CCCGGCCATC CTGGCCTCCC AGGACAGAAG GGCGACTGTG GAAAGCCAGG CCCCCAGGGC 
AGCAGTGGCC GGCCCGGAGC AGAGGGTGAG CCTGGTGCCA TGGGACCCCA GGGAAGACCC 
GGCCCCCCTG GCCATGTTGG GCCGCCGGGG CCTCCAGGCC AGCCAGGCCC AGCTGGGATC 
TCTGCAGTGG GCCCAAAAGG AGACCGTGGA GCCACTGGAG AAAGGGGCCT TTTAGGTCTT 
CCAGGCCAAC CTGGCCCCCC TGGACACCCT GGTCCCCCGG GTGAACCCGG CATGGATGGC 
GCAGCTGGCA AAGAGGGACC CCCTGGAAAA CAGGGACTCT ATGGACCCCC AGGTCCAAAG 
GGTGACCCGG GGCCTTCAGG ACAGAAGGGC CAGGCAGGAG AGAAGGGAAG AGCTGGCATG 
CCTGGCGGGC CCGGCAAGAG TGGCTCCATG GGACCTGCGG GGCCACCGGG TCCTGCAGGA 
GAGAGAGGCC ACCCTGGATC TCCGGGGCCT GCGGGGAGCC CTGGATTGCC CGGGGTGCCC 
GGCTCCATGG GAGACATGGT GAATTATGAT GAAATCAAGA GGTTCATCAG ACAAGAGATC 
ATTAAAATGT TTGATGAGAG GATGGCTTAC TACACCTCCA GGATGCAGTT TCCCATGGAG 
ATGGCAGCAG CTCCAGGGCG ACCAGGGCCC CCAGGGAAGG ATGGTCCCCC AGGCCGGCCA 
GGCTCCCCGG GATCACCGGG ACTCCCCGGT CAGATTGGCA GAGAAGGACG GCAGGGCTTG 
CCCGGGATGA GAGGGTTGCC TGGTACCAAA GGTGAAAAGG GAGACATCGG TGTTGGCATC 
GCAGGAGAAA TTGGTCTTCC TGGCCCCCCA GGTCCCCAGG GTCCTCCAGG GTACGGCAAG 
ATGGGTGCGA CTGGGCCGAT GGGCCAGCAA GGCATTCCTG GCATCCCCGG TCCCCCAGGC 
CCCATAGGCC CGCCGGGCAA GACCGGCCAC TGTAACCCCT CTGACTGCTT TGGGGCCATG 
CCGATGGAGC AACAGTACCC GCCCATGAAA AACATGAAGG GACCTTTCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203057.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203114.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X5, mRNA.

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CloneID	OBa227873
Clone ID Related Accession (Same CDS sequence)	XM_005203057.3
Accession Version	XM_005203057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTCCT 
CCGGGTGTTC CTGGACCACC AGGCCCTCCT GGAATGCCTG GGCTGCAGGG GGTGCCCGGG 
CACAACGGTT TGCCAGGACA GCCCGGGCTA ACTGCAGAAC TGGGATCCTT GCCCATTGAA 
CAGCACCTCC TGAAGAGCAT CTGCGGGGAC TGTATCCAGG GCAAGATGGC TCACGAGGTG 
GCCCTCATGG AAAGCGGAGA GAAGGGAGAC CAGGGCATCC CCGGCGTGCC AGGCCTCGAC 
AGTTGCACCC AGTGCTTCAT GGAGCGGGAG CGCCCGAGAG CCGAGGAGGC CCGGGGGGAC 
AACCATGAGG GAGACCCGGG CTGTGCAGGG AGCCCCGGGC TGCCGGGACC TCCGGGACTG 
CCTGGCCAGA GAGGAGAAGA GGGTCCACCT GGTATGAGGG GCTCCCCGGG TCCTCCAGGC 
CCTATTGGCC CCCCAGGTTT TCCTGGTGCT GTTGGCTCCC CCGGATTGCC TGGTCTTCAA 
GGAGAGCGAG GCCTCAGGGG CCTGACGGGA GAGAAGGGGG AGCCGGGTCC TCCAGGGCAA 
CCTGGTTACC CAGGCGCCAT GGGCCCCCCA GGACTGCCTG GCATCAAGGG GGAGCGAGGC 
TACGCGGGTT CCCCGGGAGA GAAGGGAGAA TCGGGCCCAC CAGGATCTGA AGGCCTCCCA 
GGGCCCCCAG GCCCAGCGGG TCCCCGAGGA GAGCGAGGGC CCCAAGGAAG CTCGGGTGAG 
AAAGGAGACC AGGGATTCCA AGGGCAACCC GGCTTTCCAG GCCCCCCGGG TCCCCCTGGA 
TTCCCAGGCA AAGCTGGAGC ACCTGGCCCA CCCGGCCCCC AAGCGGAGAA GGGCAGTGAA 
GGGATTCGAG GCCCATCAGG TCTGCCTGGT CCCCCTGGAC CACCAGGATC TCCCGGGATT 
CAGGGCCCCG CCGGTCTGGA TGGTCTGGAT GGGAAGGATG GCAAACCTGG CTTGAGGGGG 
GACCCTGGTC CTGCTGGCCC TCCCGGACTC ATGGGACCCC CGGGCTTCAA GGGAAAACCC 
GGCCATCCTG GCCTCCCAGG ACAGAAGGGC GACTGTGGAA AGCCAGGCCC CCAGGGCAGC 
AGTGGCCGGC CCGGAGCAGA GGGTGAGCCT GGTGCCATGG GACCCCAGGG AAGACCCGGC 
CCCCCTGGCC ATGTTGGGCC GCCGGGGCCT CCAGGCCAGC CAGGCCCAGC TGGGATCTCT 
GCAGTGGGCC CAAAAGGAGA CCGTGGAGCC ACTGGAGAAA GGGGCCTTTT AGGTCTTCCA 
GGCCAACCTG GCCCCCCTGG ACACCCTGGT CCCCCGGGTG AACCCGGCAT GGATGGCGCA 
GCTGGCAAAG AGGGACCCCC TGGAAAACAG GGACTCTATG GACCCCCAGG TCCAAAGGGT 
GACCCGGGGC CTTCAGGACA GAAGGGCCAG GCAGGAGAGA AGGGAAGAGC TGGCATGCCT 
GGCGGGCCCG GCAAGAGTGG CTCCATGGGA CCTGCGGGGC CACCGGGTCC TGCAGGAGAG 
AGAGGCCACC CTGGATCTCC GGGGCCTGCG GGGAGCCCTG GATTGCCCGG GGTGCCCGGC 
TCCATGGGAG ACATGGTGAA TTATGATGAA ATCAAGAGGT TCATCAGACA AGAGATCATT 
AAAATGTTTG ATGAGAGGAT GGCTTACTAC ACCTCCAGGA TGCAGTTTCC CATGGAGATG 
GCAGCAGCTC CAGGGCGACC AGGGCCCCCA GGGAAGGATG GTCCCCCAGG CCGGCCAGGC 
TCCCCGGGAT CACCGGGACT CCCCGGTCAG ATTGGCAGAG AAGGACGGCA GGGCTTGCCC 
GGGATGAGAG GGTTGCCTGG TACCAAAGGT GAAAAGGGAG ACATCGGTGT TGGCATCGCA 
GGAGAAATTG GTCTTCCTGG CCCCCCAGGT CCCCAGGGTC CTCCAGGGTA CGGCAAGATG 
GGTGCGACTG GGCCGATGGG CCAGCAAGGC ATTCCTGGCA TCCCCGGTCC CCCAGGCCCC 
ATAGGCCCGC CGGGCAAGAC CGGCCACTGT AACCCCTCTG ACTGCTTTGG GGCCATGCCG 
ATGGAGCAAC AGTACCCGCC CATGAAAAAC ATGAAGGGAC CTTTCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203114.2
CDS	234..5084
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203057.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203057.3

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTCCT 
CCGGGTGTTC CTGGACCACC AGGCCCTCCT GGAATGCCTG GGCTGCAGGG GGTGCCCGGG 
CACAACGGTT TGCCAGGACA GCCCGGGCTA ACTGCAGAAC TGGGATCCTT GCCCATTGAA 
CAGCACCTCC TGAAGAGCAT CTGCGGGGAC TGTATCCAGG GCAAGATGGC TCACGAGGTG 
GCCCTCATGG AAAGCGGAGA GAAGGGAGAC CAGGGCATCC CCGGCGTGCC AGGCCTCGAC 
AGTTGCACCC AGTGCTTCAT GGAGCGGGAG CGCCCGAGAG CCGAGGAGGC CCGGGGGGAC 
AACCATGAGG GAGACCCGGG CTGTGCAGGG AGCCCCGGGC TGCCGGGACC TCCGGGACTG 
CCTGGCCAGA GAGGAGAAGA GGGTCCACCT GGTATGAGGG GCTCCCCGGG TCCTCCAGGC 
CCTATTGGCC CCCCAGGTTT TCCTGGTGCT GTTGGCTCCC CCGGATTGCC TGGTCTTCAA 
GGAGAGCGAG GCCTCAGGGG CCTGACGGGA GAGAAGGGGG AGCCGGGTCC TCCAGGGCAA 
CCTGGTTACC CAGGCGCCAT GGGCCCCCCA GGACTGCCTG GCATCAAGGG GGAGCGAGGC 
TACGCGGGTT CCCCGGGAGA GAAGGGAGAA TCGGGCCCAC CAGGATCTGA AGGCCTCCCA 
GGGCCCCCAG GCCCAGCGGG TCCCCGAGGA GAGCGAGGGC CCCAAGGAAG CTCGGGTGAG 
AAAGGAGACC AGGGATTCCA AGGGCAACCC GGCTTTCCAG GCCCCCCGGG TCCCCCTGGA 
TTCCCAGGCA AAGCTGGAGC ACCTGGCCCA CCCGGCCCCC AAGCGGAGAA GGGCAGTGAA 
GGGATTCGAG GCCCATCAGG TCTGCCTGGT CCCCCTGGAC CACCAGGATC TCCCGGGATT 
CAGGGCCCCG CCGGTCTGGA TGGTCTGGAT GGGAAGGATG GCAAACCTGG CTTGAGGGGG 
GACCCTGGTC CTGCTGGCCC TCCCGGACTC ATGGGACCCC CGGGCTTCAA GGGAAAACCC 
GGCCATCCTG GCCTCCCAGG ACAGAAGGGC GACTGTGGAA AGCCAGGCCC CCAGGGCAGC 
AGTGGCCGGC CCGGAGCAGA GGGTGAGCCT GGTGCCATGG GACCCCAGGG AAGACCCGGC 
CCCCCTGGCC ATGTTGGGCC GCCGGGGCCT CCAGGCCAGC CAGGCCCAGC TGGGATCTCT 
GCAGTGGGCC CAAAAGGAGA CCGTGGAGCC ACTGGAGAAA GGGGCCTTTT AGGTCTTCCA 
GGCCAACCTG GCCCCCCTGG ACACCCTGGT CCCCCGGGTG AACCCGGCAT GGATGGCGCA 
GCTGGCAAAG AGGGACCCCC TGGAAAACAG GGACTCTATG GACCCCCAGG TCCAAAGGGT 
GACCCGGGGC CTTCAGGACA GAAGGGCCAG GCAGGAGAGA AGGGAAGAGC TGGCATGCCT 
GGCGGGCCCG GCAAGAGTGG CTCCATGGGA CCTGCGGGGC CACCGGGTCC TGCAGGAGAG 
AGAGGCCACC CTGGATCTCC GGGGCCTGCG GGGAGCCCTG GATTGCCCGG GGTGCCCGGC 
TCCATGGGAG ACATGGTGAA TTATGATGAA ATCAAGAGGT TCATCAGACA AGAGATCATT 
AAAATGTTTG ATGAGAGGAT GGCTTACTAC ACCTCCAGGA TGCAGTTTCC CATGGAGATG 
GCAGCAGCTC CAGGGCGACC AGGGCCCCCA GGGAAGGATG GTCCCCCAGG CCGGCCAGGC 
TCCCCGGGAT CACCGGGACT CCCCGGTCAG ATTGGCAGAG AAGGACGGCA GGGCTTGCCC 
GGGATGAGAG GGTTGCCTGG TACCAAAGGT GAAAAGGGAG ACATCGGTGT TGGCATCGCA 
GGAGAAATTG GTCTTCCTGG CCCCCCAGGT CCCCAGGGTC CTCCAGGGTA CGGCAAGATG 
GGTGCGACTG GGCCGATGGG CCAGCAAGGC ATTCCTGGCA TCCCCGGTCC CCCAGGCCCC 
ATAGGCCCGC CGGGCAAGAC CGGCCACTGT AACCCCTCTG ACTGCTTTGG GGCCATGCCG 
ATGGAGCAAC AGTACCCGCC CATGAAAAAC ATGAAGGGAC CTTTCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_024982333.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838101.1 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X6, mRNA.

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CloneID	OBa227874
Clone ID Related Accession (Same CDS sequence)	XM_024982333.1
Accession Version	XM_024982333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTCCTC CGGGTGTTCC TGGACCACCA 
GGCCCTCCTG GAATGCCTGG GCTGCAGGGG GTGCCCGGGC ACAACGGTTT GCCAGGACAG 
CCCGGGCTAA CTGCAGAACT GGGATCCTTG CCCATTGAAC AGCACCTCCT GAAGAGCATC 
TGCGGGGACT GTATCCAGGG CAAGATGGCT CACGAGGTGG CCCTCATGGA AAGCGGAGAG 
AAGGGAGACC AGGGCATCCC CGGCGTGCCA GGCCTCGACA GTTGCACCCA GTGCTTCATG 
GAGCGGGAGC GCCCGAGAGC CGAGGAGGCC CGGGGGGACA ACCATGAGGG AGACCCGGGC 
TGTGCAGGGA GCCCCGGGCT GCCGGGACCT CCGGGACTGC CTGGCCAGAG AGGAGAAGAG 
GGTCCACCTG GTATGAGGGG CTCCCCGGGT CCTCCAGGCC CTATTGGCCC CCCAGGTTTT 
CCTGGTGCTG TTGGCTCCCC CGGATTGCCT GGTCTTCAAG GAGAGCGAGG CCTCAGGGGC 
CTGACGGGAG AGAAGGGGGA GCCGGGTCCT CCAGGGCAAC CTGGTTACCC AGGCGCCATG 
GGCCCCCCAG GACTGCCTGG CATCAAGGGG GAGCGAGGCT ACGCGGGTTC CCCGGGAGAG 
AAGGGAGAAT CGGGCCCACC AGGATCTGAA GGCCTCCCAG GGCCCCCAGG CCCAGCGGGT 
CCCCGAGGAG AGCGAGGGCC CCAAGGAAGC TCGGGTGAGA AAGGAGACCA GGGATTCCAA 
GGGCAACCCG GCTTTCCAGG CCCCCCGGGT CCCCCTGGAT TCCCAGGCAA AGCTGGAGCA 
CCTGGCCCAC CCGGCCCCCA AGCGGAGAAG GGCAGTGAAG GGATTCGAGG CCCATCAGGT 
CTGCCTGGTC CCCCTGGACC ACCAGGATCT CCCGGGATTC AGGGCCCCGC CGGTCTGGAT 
GGTCTGGATG GGAAGGATGG CAAACCTGGC TTGAGGGGGG ACCCTGGTCC TGCTGGCCCT 
CCCGGACTCA TGGGACCCCC GGGCTTCAAG GGAAAACCCG GCCATCCTGG CCTCCCAGGA 
CAGAAGGGCG ACTGTGGAAA GCCAGGCCCC CAGGGCAGCA GTGGCCGGCC CGGAGCAGAG 
GGTGAGCCTG GTGCCATGGG ACCCCAGGGA AGACCCGGCC CCCCTGGCCA TGTTGGGCCG 
CCGGGGCCTC CAGGCCAGCC AGGCCCAGCT GGGATCTCTG CAGTGGGCCC AAAAGGAGAC 
CGTGGAGCCA CTGGAGAAAG GGGCCTTTTA GGTCTTCCAG GCCAACCTGG CCCCCCTGGA 
CACCCTGGTC CCCCGGGTGA ACCCGGCATG GATGGCGCAG CTGGCAAAGA GGGACCCCCT 
GGAAAACAGG GACTCTATGG ACCCCCAGGT CCAAAGGGTG ACCCGGGGCC TTCAGGACAG 
AAGGGCCAGG CAGGAGAGAA GGGAAGAGCT GGCATGCCTG GCGGGCCCGG CAAGAGTGGC 
TCCATGGGAC CTGCGGGGCC ACCGGGTCCT GCAGGAGAGA GAGGCCACCC TGGATCTCCG 
GGGCCTGCGG GGAGCCCTGG ATTGCCCGGG GTGCCCGGCT CCATGGGAGA CATGGTGAAT 
TATGATGAAA TCAAGAGGTT CATCAGACAA GAGATCATTA AAATGTTTGA TGAGAGGATG 
GCTTACTACA CCTCCAGGAT GCAGTTTCCC ATGGAGATGG CAGCAGCTCC AGGGCGACCA 
GGGCCCCCAG GGAAGGATGG TCCCCCAGGC CGGCCAGGCT CCCCGGGATC ACCGGGACTC 
CCCGGTCAGA TTGGCAGAGA AGGACGGCAG GGCTTGCCCG GGATGAGAGG GTTGCCTGGT 
ACCAAAGGTG AAAAGGGAGA CATCGGTGTT GGCATCGCAG GAGAAATTGG TCTTCCTGGC 
CCCCCAGGTC CCCAGGGTCC TCCAGGGTAC GGCAAGATGG GTGCGACTGG GCCGATGGGC 
CAGCAAGGCA TTCCTGGCAT CCCCGGTCCC CCAGGCCCCA TAGGCCCGCC GGGCAAGACC 
GGCCACTGTA ACCCCTCTGA CTGCTTTGGG GCCATGCCGA TGGAGCAACA GTACCCGCCC 
ATGAAAAACA TGAAGGGACC TTTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838101.1
CDS	320..5149
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_024982333.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982333.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTCCTC CGGGTGTTCC TGGACCACCA 
GGCCCTCCTG GAATGCCTGG GCTGCAGGGG GTGCCCGGGC ACAACGGTTT GCCAGGACAG 
CCCGGGCTAA CTGCAGAACT GGGATCCTTG CCCATTGAAC AGCACCTCCT GAAGAGCATC 
TGCGGGGACT GTATCCAGGG CAAGATGGCT CACGAGGTGG CCCTCATGGA AAGCGGAGAG 
AAGGGAGACC AGGGCATCCC CGGCGTGCCA GGCCTCGACA GTTGCACCCA GTGCTTCATG 
GAGCGGGAGC GCCCGAGAGC CGAGGAGGCC CGGGGGGACA ACCATGAGGG AGACCCGGGC 
TGTGCAGGGA GCCCCGGGCT GCCGGGACCT CCGGGACTGC CTGGCCAGAG AGGAGAAGAG 
GGTCCACCTG GTATGAGGGG CTCCCCGGGT CCTCCAGGCC CTATTGGCCC CCCAGGTTTT 
CCTGGTGCTG TTGGCTCCCC CGGATTGCCT GGTCTTCAAG GAGAGCGAGG CCTCAGGGGC 
CTGACGGGAG AGAAGGGGGA GCCGGGTCCT CCAGGGCAAC CTGGTTACCC AGGCGCCATG 
GGCCCCCCAG GACTGCCTGG CATCAAGGGG GAGCGAGGCT ACGCGGGTTC CCCGGGAGAG 
AAGGGAGAAT CGGGCCCACC AGGATCTGAA GGCCTCCCAG GGCCCCCAGG CCCAGCGGGT 
CCCCGAGGAG AGCGAGGGCC CCAAGGAAGC TCGGGTGAGA AAGGAGACCA GGGATTCCAA 
GGGCAACCCG GCTTTCCAGG CCCCCCGGGT CCCCCTGGAT TCCCAGGCAA AGCTGGAGCA 
CCTGGCCCAC CCGGCCCCCA AGCGGAGAAG GGCAGTGAAG GGATTCGAGG CCCATCAGGT 
CTGCCTGGTC CCCCTGGACC ACCAGGATCT CCCGGGATTC AGGGCCCCGC CGGTCTGGAT 
GGTCTGGATG GGAAGGATGG CAAACCTGGC TTGAGGGGGG ACCCTGGTCC TGCTGGCCCT 
CCCGGACTCA TGGGACCCCC GGGCTTCAAG GGAAAACCCG GCCATCCTGG CCTCCCAGGA 
CAGAAGGGCG ACTGTGGAAA GCCAGGCCCC CAGGGCAGCA GTGGCCGGCC CGGAGCAGAG 
GGTGAGCCTG GTGCCATGGG ACCCCAGGGA AGACCCGGCC CCCCTGGCCA TGTTGGGCCG 
CCGGGGCCTC CAGGCCAGCC AGGCCCAGCT GGGATCTCTG CAGTGGGCCC AAAAGGAGAC 
CGTGGAGCCA CTGGAGAAAG GGGCCTTTTA GGTCTTCCAG GCCAACCTGG CCCCCCTGGA 
CACCCTGGTC CCCCGGGTGA ACCCGGCATG GATGGCGCAG CTGGCAAAGA GGGACCCCCT 
GGAAAACAGG GACTCTATGG ACCCCCAGGT CCAAAGGGTG ACCCGGGGCC TTCAGGACAG 
AAGGGCCAGG CAGGAGAGAA GGGAAGAGCT GGCATGCCTG GCGGGCCCGG CAAGAGTGGC 
TCCATGGGAC CTGCGGGGCC ACCGGGTCCT GCAGGAGAGA GAGGCCACCC TGGATCTCCG 
GGGCCTGCGG GGAGCCCTGG ATTGCCCGGG GTGCCCGGCT CCATGGGAGA CATGGTGAAT 
TATGATGAAA TCAAGAGGTT CATCAGACAA GAGATCATTA AAATGTTTGA TGAGAGGATG 
GCTTACTACA CCTCCAGGAT GCAGTTTCCC ATGGAGATGG CAGCAGCTCC AGGGCGACCA 
GGGCCCCCAG GGAAGGATGG TCCCCCAGGC CGGCCAGGCT CCCCGGGATC ACCGGGACTC 
CCCGGTCAGA TTGGCAGAGA AGGACGGCAG GGCTTGCCCG GGATGAGAGG GTTGCCTGGT 
ACCAAAGGTG AAAAGGGAGA CATCGGTGTT GGCATCGCAG GAGAAATTGG TCTTCCTGGC 
CCCCCAGGTC CCCAGGGTCC TCCAGGGTAC GGCAAGATGG GTGCGACTGG GCCGATGGGC 
CAGCAAGGCA TTCCTGGCAT CCCCGGTCCC CCAGGCCCCA TAGGCCCGCC GGGCAAGACC 
GGCCACTGTA ACCCCTCTGA CTGCTTTGGG GCCATGCCGA TGGAGCAACA GTACCCGCCC 
ATGAAAAACA TGAAGGGACC TTTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015459964.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315450.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X7, mRNA.

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CloneID	OBa227875
Clone ID Related Accession (Same CDS sequence)	XM_015459964.2
Accession Version	XM_015459964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCTTCAAGG AGAGCGAGGC CTCAGGGGCC TGACGGGAGA GAAGGGGGAG 
CCGGGTCCTC CAGGGCAACC TGGTTACCCA GGCGCCATGG GCCCCCCAGG ACTGCCTGGC 
ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC CCGGGAGAGA AGGGAGAATC GGGCCCACCA 
GGATCTGAAG GCCTCCCAGG GCCCCCAGGC CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC 
CAAGGAAGCT CGGGTGAGAA AGGAGACCAG GGATTCCAAG GGCAACCCGG CTTTCCAGGC 
CCCCCGGGTC CCCCTGGATT CCCAGGCAAA GCTGGAGCAC CTGGCCCACC CGGCCCCCAA 
GCGGAGAAGG GCAGTGAAGG GATTCGAGGC CCATCAGGTC TGCCTGGTCC CCCTGGACCA 
CCAGGATCTC CCGGGATTCA GGGCCCCGCC GGTCTGGATG GTCTGGATGG GAAGGATGGC 
AAACCTGGCT TGAGGGGGGA CCCTGGTCCT GCTGGCCCTC CCGGACTCAT GGGACCCCCG 
GGCTTCAAGG GAAAACCCGG CCATCCTGGC CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG 
CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC GGAGCAGAGG GTGAGCCTGG TGCCATGGGA 
CCCCAGGGAA GACCCGGCCC CCCTGGCCAT GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA 
GGCCCAGCTG GGATCTCTGC AGTGGGCCCA AAAGGAGACC GTGGAGCCAC TGGAGAAAGG 
GGCCTTTTAG GTCTTCCAGG CCAACCTGGC CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA 
CCCGGCATGG ATGGCGCAGC TGGCAAAGAG GGACCCCCTG GAAAACAGGG ACTCTATGGA 
CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT TCAGGACAGA AGGGCCAGGC AGGAGAGAAG 
GGAAGAGCTG GCATGCCTGG CGGGCCCGGC AAGAGTGGCT CCATGGGACC TGCGGGGCCA 
CCGGGTCCTG CAGGAGAGAG AGGCCACCCT GGATCTCCGG GGCCTGCGGG GAGCCCTGGA 
TTGCCCGGGG TGCCCGGCTC CATGGGAGAC ATGGTGAATT ATGATGAAAT CAAGAGGTTC 
ATCAGACAAG AGATCATTAA AATGTTTGAT GAGAGGATGG CTTACTACAC CTCCAGGATG 
CAGTTTCCCA TGGAGATGGC AGCAGCTCCA GGGCGACCAG GGCCCCCAGG GAAGGATGGT 
CCCCCAGGCC GGCCAGGCTC CCCGGGATCA CCGGGACTCC CCGGTCAGAT TGGCAGAGAA 
GGACGGCAGG GCTTGCCCGG GATGAGAGGG TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC 
ATCGGTGTTG GCATCGCAGG AGAAATTGGT CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT 
CCAGGGTACG GCAAGATGGG TGCGACTGGG CCGATGGGCC AGCAAGGCAT TCCTGGCATC 
CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG GGCAAGACCG GCCACTGTAA CCCCTCTGAC 
TGCTTTGGGG CCATGCCGAT GGAGCAACAG TACCCGCCCA TGAAAAACAT GAAGGGACCT 
TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315450.2
CDS	247..5055
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015459964.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459964.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCTTCAAGG AGAGCGAGGC CTCAGGGGCC TGACGGGAGA GAAGGGGGAG 
CCGGGTCCTC CAGGGCAACC TGGTTACCCA GGCGCCATGG GCCCCCCAGG ACTGCCTGGC 
ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC CCGGGAGAGA AGGGAGAATC GGGCCCACCA 
GGATCTGAAG GCCTCCCAGG GCCCCCAGGC CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC 
CAAGGAAGCT CGGGTGAGAA AGGAGACCAG GGATTCCAAG GGCAACCCGG CTTTCCAGGC 
CCCCCGGGTC CCCCTGGATT CCCAGGCAAA GCTGGAGCAC CTGGCCCACC CGGCCCCCAA 
GCGGAGAAGG GCAGTGAAGG GATTCGAGGC CCATCAGGTC TGCCTGGTCC CCCTGGACCA 
CCAGGATCTC CCGGGATTCA GGGCCCCGCC GGTCTGGATG GTCTGGATGG GAAGGATGGC 
AAACCTGGCT TGAGGGGGGA CCCTGGTCCT GCTGGCCCTC CCGGACTCAT GGGACCCCCG 
GGCTTCAAGG GAAAACCCGG CCATCCTGGC CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG 
CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC GGAGCAGAGG GTGAGCCTGG TGCCATGGGA 
CCCCAGGGAA GACCCGGCCC CCCTGGCCAT GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA 
GGCCCAGCTG GGATCTCTGC AGTGGGCCCA AAAGGAGACC GTGGAGCCAC TGGAGAAAGG 
GGCCTTTTAG GTCTTCCAGG CCAACCTGGC CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA 
CCCGGCATGG ATGGCGCAGC TGGCAAAGAG GGACCCCCTG GAAAACAGGG ACTCTATGGA 
CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT TCAGGACAGA AGGGCCAGGC AGGAGAGAAG 
GGAAGAGCTG GCATGCCTGG CGGGCCCGGC AAGAGTGGCT CCATGGGACC TGCGGGGCCA 
CCGGGTCCTG CAGGAGAGAG AGGCCACCCT GGATCTCCGG GGCCTGCGGG GAGCCCTGGA 
TTGCCCGGGG TGCCCGGCTC CATGGGAGAC ATGGTGAATT ATGATGAAAT CAAGAGGTTC 
ATCAGACAAG AGATCATTAA AATGTTTGAT GAGAGGATGG CTTACTACAC CTCCAGGATG 
CAGTTTCCCA TGGAGATGGC AGCAGCTCCA GGGCGACCAG GGCCCCCAGG GAAGGATGGT 
CCCCCAGGCC GGCCAGGCTC CCCGGGATCA CCGGGACTCC CCGGTCAGAT TGGCAGAGAA 
GGACGGCAGG GCTTGCCCGG GATGAGAGGG TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC 
ATCGGTGTTG GCATCGCAGG AGAAATTGGT CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT 
CCAGGGTACG GCAAGATGGG TGCGACTGGG CCGATGGGCC AGCAAGGCAT TCCTGGCATC 
CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG GGCAAGACCG GCCACTGTAA CCCCTCTGAC 
TGCTTTGGGG CCATGCCGAT GGAGCAACAG TACCCGCCCA TGAAAAACAT GAAGGGACCT 
TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_010802506.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800808.2 Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X8, mRNA.

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CloneID	OBa227876
Clone ID Related Accession (Same CDS sequence)	XM_010802506.2
Accession Version	XM_010802506.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGTCTTC AAGGAGAGCG AGGCCTCAGG 
GGCCTGACGG GAGAGAAGGG GGAGCCGGGT CCTCCAGGGC AACCTGGTTA CCCAGGCGCC 
ATGGGCCCCC CAGGACTGCC TGGCATCAAG GGGGAGCGAG GCTACGCGGG TTCCCCGGGA 
GAGAAGGGAG AATCGGGCCC ACCAGGATCT GAAGGCCTCC CAGGGCCCCC AGGCCCAGCG 
GGTCCCCGAG GAGAGCGAGG GCCCCAAGGA AGCTCGGGTG AGAAAGGAGA CCAGGGATTC 
CAAGGGCAAC CCGGCTTTCC AGGCCCCCCG GGTCCCCCTG GATTCCCAGG CAAAGCTGGA 
GCACCTGGCC CACCCGGCCC CCAAGCGGAG AAGGGCAGTG AAGGGATTCG AGGCCCATCA 
GGTCTGCCTG GTCCCCCTGG ACCACCAGGA TCTCCCGGGA TTCAGGGCCC CGCCGGTCTG 
GATGGTCTGG ATGGGAAGGA TGGCAAACCT GGCTTGAGGG GGGACCCTGG TCCTGCTGGC 
CCTCCCGGAC TCATGGGACC CCCGGGCTTC AAGGGAAAAC CCGGCCATCC TGGCCTCCCA 
GGACAGAAGG GCGACTGTGG AAAGCCAGGC CCCCAGGGCA GCAGTGGCCG GCCCGGAGCA 
GAGGGTGAGC CTGGTGCCAT GGGACCCCAG GGAAGACCCG GCCCCCCTGG CCATGTTGGG 
CCGCCGGGGC CTCCAGGCCA GCCAGGCCCA GCTGGGATCT CTGCAGTGGG CCCAAAAGGA 
GACCGTGGAG CCACTGGAGA AAGGGGCCTT TTAGGTCTTC CAGGCCAACC TGGCCCCCCT 
GGACACCCTG GTCCCCCGGG TGAACCCGGC ATGGATGGCG CAGCTGGCAA AGAGGGACCC 
CCTGGAAAAC AGGGACTCTA TGGACCCCCA GGTCCAAAGG GTGACCCGGG GCCTTCAGGA 
CAGAAGGGCC AGGCAGGAGA GAAGGGAAGA GCTGGCATGC CTGGCGGGCC CGGCAAGAGT 
GGCTCCATGG GACCTGCGGG GCCACCGGGT CCTGCAGGAG AGAGAGGCCA CCCTGGATCT 
CCGGGGCCTG CGGGGAGCCC TGGATTGCCC GGGGTGCCCG GCTCCATGGG AGACATGGTG 
AATTATGATG AAATCAAGAG GTTCATCAGA CAAGAGATCA TTAAAATGTT TGATGAGAGG 
ATGGCTTACT ACACCTCCAG GATGCAGTTT CCCATGGAGA TGGCAGCAGC TCCAGGGCGA 
CCAGGGCCCC CAGGGAAGGA TGGTCCCCCA GGCCGGCCAG GCTCCCCGGG ATCACCGGGA 
CTCCCCGGTC AGATTGGCAG AGAAGGACGG CAGGGCTTGC CCGGGATGAG AGGGTTGCCT 
GGTACCAAAG GTGAAAAGGG AGACATCGGT GTTGGCATCG CAGGAGAAAT TGGTCTTCCT 
GGCCCCCCAG GTCCCCAGGG TCCTCCAGGG TACGGCAAGA TGGGTGCGAC TGGGCCGATG 
GGCCAGCAAG GCATTCCTGG CATCCCCGGT CCCCCAGGCC CCATAGGCCC GCCGGGCAAG 
ACCGGCCACT GTAACCCCTC TGACTGCTTT GGGGCCATGC CGATGGAGCA ACAGTACCCG 
CCCATGAAAA ACATGAAGGG ACCTTTCGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800808.2
CDS	247..5079
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_010802506.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 chain (COL16A1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802506.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGTCTTC AAGGAGAGCG AGGCCTCAGG 
GGCCTGACGG GAGAGAAGGG GGAGCCGGGT CCTCCAGGGC AACCTGGTTA CCCAGGCGCC 
ATGGGCCCCC CAGGACTGCC TGGCATCAAG GGGGAGCGAG GCTACGCGGG TTCCCCGGGA 
GAGAAGGGAG AATCGGGCCC ACCAGGATCT GAAGGCCTCC CAGGGCCCCC AGGCCCAGCG 
GGTCCCCGAG GAGAGCGAGG GCCCCAAGGA AGCTCGGGTG AGAAAGGAGA CCAGGGATTC 
CAAGGGCAAC CCGGCTTTCC AGGCCCCCCG GGTCCCCCTG GATTCCCAGG CAAAGCTGGA 
GCACCTGGCC CACCCGGCCC CCAAGCGGAG AAGGGCAGTG AAGGGATTCG AGGCCCATCA 
GGTCTGCCTG GTCCCCCTGG ACCACCAGGA TCTCCCGGGA TTCAGGGCCC CGCCGGTCTG 
GATGGTCTGG ATGGGAAGGA TGGCAAACCT GGCTTGAGGG GGGACCCTGG TCCTGCTGGC 
CCTCCCGGAC TCATGGGACC CCCGGGCTTC AAGGGAAAAC CCGGCCATCC TGGCCTCCCA 
GGACAGAAGG GCGACTGTGG AAAGCCAGGC CCCCAGGGCA GCAGTGGCCG GCCCGGAGCA 
GAGGGTGAGC CTGGTGCCAT GGGACCCCAG GGAAGACCCG GCCCCCCTGG CCATGTTGGG 
CCGCCGGGGC CTCCAGGCCA GCCAGGCCCA GCTGGGATCT CTGCAGTGGG CCCAAAAGGA 
GACCGTGGAG CCACTGGAGA AAGGGGCCTT TTAGGTCTTC CAGGCCAACC TGGCCCCCCT 
GGACACCCTG GTCCCCCGGG TGAACCCGGC ATGGATGGCG CAGCTGGCAA AGAGGGACCC 
CCTGGAAAAC AGGGACTCTA TGGACCCCCA GGTCCAAAGG GTGACCCGGG GCCTTCAGGA 
CAGAAGGGCC AGGCAGGAGA GAAGGGAAGA GCTGGCATGC CTGGCGGGCC CGGCAAGAGT 
GGCTCCATGG GACCTGCGGG GCCACCGGGT CCTGCAGGAG AGAGAGGCCA CCCTGGATCT 
CCGGGGCCTG CGGGGAGCCC TGGATTGCCC GGGGTGCCCG GCTCCATGGG AGACATGGTG 
AATTATGATG AAATCAAGAG GTTCATCAGA CAAGAGATCA TTAAAATGTT TGATGAGAGG 
ATGGCTTACT ACACCTCCAG GATGCAGTTT CCCATGGAGA TGGCAGCAGC TCCAGGGCGA 
CCAGGGCCCC CAGGGAAGGA TGGTCCCCCA GGCCGGCCAG GCTCCCCGGG ATCACCGGGA 
CTCCCCGGTC AGATTGGCAG AGAAGGACGG CAGGGCTTGC CCGGGATGAG AGGGTTGCCT 
GGTACCAAAG GTGAAAAGGG AGACATCGGT GTTGGCATCG CAGGAGAAAT TGGTCTTCCT 
GGCCCCCCAG GTCCCCAGGG TCCTCCAGGG TACGGCAAGA TGGGTGCGAC TGGGCCGATG 
GGCCAGCAAG GCATTCCTGG CATCCCCGGT CCCCCAGGCC CCATAGGCCC GCCGGGCAAG 
ACCGGCCACT GTAACCCCTC TGACTGCTTT GGGGCCATGC CGATGGAGCA ACAGTACCCG 
CCCATGAAAA ACATGAAGGG ACCTTTCGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203054.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203111.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa16873
Clone ID Related Accession (Same CDS sequence)	XM_005203054.2 , XM_015462426.1 , XM_005203053.2 , XM_015462425.1
Accession Version	XM_005203054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203111.1
CDS	227..5125
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203054.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203054.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462426.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317912.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X2, mRNA.

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CloneID	OBa16873
Clone ID Related Accession (Same CDS sequence)	XM_005203054.2 , XM_015462426.1 , XM_005203053.2 , XM_015462425.1
Accession Version	XM_015462426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317912.1
CDS	342..5240
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462426.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462426.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203053.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203110.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X1, mRNA.

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CloneID	OBa16873
Clone ID Related Accession (Same CDS sequence)	XM_005203054.2 , XM_015462426.1 , XM_005203053.2 , XM_015462425.1
Accession Version	XM_005203053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005203053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203110.1
CDS	106..5004
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203053.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203053.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462425.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317911.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X1, mRNA.

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CloneID	OBa16873
Clone ID Related Accession (Same CDS sequence)	XM_005203054.2 , XM_015462426.1 , XM_005203053.2 , XM_015462425.1
Accession Version	XM_015462425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317911.1
CDS	207..5105
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462425.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462425.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGCCCC 
CCAGGTTTTC CTGGTGCTGT TGGCTCCCCC GGATTGCCTG GTCTTCAAGG AGAGCGAGGC 
CTCAGGGGCC TGACGGGAGA GAAGGGGGAG CCGGGTCCTC CAGGGCAACC TGGTTACCCA 
GGCGCCATGG GCCCCCCAGG ACTGCCTGGC ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC 
CCGGGAGAGA AGGGAGAATC GGGCCCACCA GGATCTGAAG GCCTCCCAGG GCCCCCAGGC 
CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC CAAGGAAGCT CGGGTGAGAA AGGAGACCAG 
GGATTCCAAG GGCAACCCGG CTTTCCAGGC CCCCCGGGTC CCCCTGGATT CCCAGGCAAA 
GCTGGAGCAC CTGGCCCACC CGGCCCCCAA GCGGATAAGG GCAGTGAAGG GATTCGAGGC 
CCATCAGGTC TGCCTGGTCC CCCTGGACCA CCAGGATCTC CCGGGATTCA GGGCCCCGCC 
GGTCTGGATG GTCTGGATGG GAAGGATGGC AAACCTGGCT TGAGGGGGGA CCCTGGTCCT 
GCTGGCCCTC CCGGACTCAT GGGACCCCCG GGCTTCAAGG GAAAACCCGG CCATCCTGGC 
CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC 
GGAGCAGAGG GTGAGCCTGG TGCCATGGGA CCCCAGGGAA GACCCGGCCC CCCTGGCCAT 
GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA GGCCCAGCTG GGATCTCTGC AGTGGGCCCA 
AAAGGAGACC GTGGAGCCAC TGGAGAAAGG GGCCTTTTAG GTCTTCCAGG CCAACCTGGC 
CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA CCCGGCATGG ATGGCGCAGC TGGCAAAGAG 
GGACCCCCTG GAAAACAGGG ACTCTATGGA CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT 
TCAGGACAGA AGGGCCAGGC AGGAGAGAAG GGAAGAGCTG GCATGCCTGG CGGGCCCGGC 
AAGAGTGGCT CCATGGGACC TGCGGGGCCA CCGGGTCCTG CAGGAGAGAG AGGCCACCCT 
GGATCTCCGG GGCCTGCGGG GAGCCCTGGA TTGCCCGGGG TGCCCGGCTC CATGGGAGAC 
ATGGTGAATT ATGATGAAAT CAAGAGGTTC ATCAGACAAG AGATCATTAA AATGTTTGAT 
GAGAGGATGG CTTACTACAC CTCCAGGATG CAGTTTCCCA TGGAGATGGC AGCAGCTCCA 
GGGCGACCAG GGCCCCCAGG GAAGGATGGT CCCCCAGGCC GGCCAGGCTC CCCGGGATCA 
CCGGGACTCC CCGGTCAGAT TGGCAGAGAA GGACGGCAGG GCTTGCCCGG GATGAGAGGG 
TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC ATCGGTGTTG GCATCGCAGG AGAAATTGGT 
CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT CCAGGGTACG GCAAGATGGG TGCGACTGGG 
CCGATGGGCC AGCAAGGCAT TCCTGGCATC CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG 
GGCAAGACCG GCCACTGTAA CCCCTCTGAC TGCTTTGGGG CCATGCCGAT GGAGCAACAG 
TACCCGCCCA TGAAAAACAT GAAGGGACCT TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203055.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203112.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X3, mRNA.

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CloneID	OBa16874
Clone ID Related Accession (Same CDS sequence)	XM_005203055.2 , XM_015462427.1
Accession Version	XM_005203055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005203055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGCCCCC CAGGTTTTCC TGGTGCTGTT 
GGCTCCCCCG GATTGCCTGG TCTTCAAGGA GAGCGAGGCC TCAGGGGCCT GACGGGAGAG 
AAGGGGGAGC CGGGTCCTCC AGGGCAACCT GGTTACCCAG GCGCCATGGG CCCCCCAGGA 
CTGCCTGGCA TCAAGGGGGA GCGAGGCTAC GCGGGTTCCC CGGGAGAGAA GGGAGAATCG 
GGCCCACCAG GATCTGAAGG CCTCCCAGGG CCCCCAGGCC CAGCGGGTCC CCGAGGAGAG 
CGAGGGCCCC AAGGAAGCTC GGGTGAGAAA GGAGACCAGG GATTCCAAGG GCAACCCGGC 
TTTCCAGGCC CCCCGGGTCC CCCTGGATTC CCAGGCAAAG CTGGAGCACC TGGCCCACCC 
GGCCCCCAAG CGGATAAGGG CAGTGAAGGG ATTCGAGGCC CATCAGGTCT GCCTGGTCCC 
CCTGGACCAC CAGGATCTCC CGGGATTCAG GGCCCCGCCG GTCTGGATGG TCTGGATGGG 
AAGGATGGCA AACCTGGCTT GAGGGGGGAC CCTGGTCCTG CTGGCCCTCC CGGACTCATG 
GGACCCCCGG GCTTCAAGGG AAAACCCGGC CATCCTGGCC TCCCAGGACA GAAGGGCGAC 
TGTGGAAAGC CAGGCCCCCA GGGCAGCAGT GGCCGGCCCG GAGCAGAGGG TGAGCCTGGT 
GCCATGGGAC CCCAGGGAAG ACCCGGCCCC CCTGGCCATG TTGGGCCGCC GGGGCCTCCA 
GGCCAGCCAG GCCCAGCTGG GATCTCTGCA GTGGGCCCAA AAGGAGACCG TGGAGCCACT 
GGAGAAAGGG GCCTTTTAGG TCTTCCAGGC CAACCTGGCC CCCCTGGACA CCCTGGTCCC 
CCGGGTGAAC CCGGCATGGA TGGCGCAGCT GGCAAAGAGG GACCCCCTGG AAAACAGGGA 
CTCTATGGAC CCCCAGGTCC AAAGGGTGAC CCGGGGCCTT CAGGACAGAA GGGCCAGGCA 
GGAGAGAAGG GAAGAGCTGG CATGCCTGGC GGGCCCGGCA AGAGTGGCTC CATGGGACCT 
GCGGGGCCAC CGGGTCCTGC AGGAGAGAGA GGCCACCCTG GATCTCCGGG GCCTGCGGGG 
AGCCCTGGAT TGCCCGGGGT GCCCGGCTCC ATGGGAGACA TGGTGAATTA TGATGAAATC 
AAGAGGTTCA TCAGACAAGA GATCATTAAA ATGTTTGATG AGAGGATGGC TTACTACACC 
TCCAGGATGC AGTTTCCCAT GGAGATGGCA GCAGCTCCAG GGCGACCAGG GCCCCCAGGG 
AAGGATGGTC CCCCAGGCCG GCCAGGCTCC CCGGGATCAC CGGGACTCCC CGGTCAGATT 
GGCAGAGAAG GACGGCAGGG CTTGCCCGGG ATGAGAGGGT TGCCTGGTAC CAAAGGTGAA 
AAGGGAGACA TCGGTGTTGG CATCGCAGGA GAAATTGGTC TTCCTGGCCC CCCAGGTCCC 
CAGGGTCCTC CAGGGTACGG CAAGATGGGT GCGACTGGGC CGATGGGCCA GCAAGGCATT 
CCTGGCATCC CCGGTCCCCC AGGCCCCATA GGCCCGCCGG GCAAGACCGG CCACTGTAAC 
CCCTCTGACT GCTTTGGGGC CATGCCGATG GAGCAACAGT ACCCGCCCAT GAAAAACATG 
AAGGGACCTT TCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203112.1
CDS	106..4983
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203055.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203055.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGCCCCC CAGGTTTTCC TGGTGCTGTT 
GGCTCCCCCG GATTGCCTGG TCTTCAAGGA GAGCGAGGCC TCAGGGGCCT GACGGGAGAG 
AAGGGGGAGC CGGGTCCTCC AGGGCAACCT GGTTACCCAG GCGCCATGGG CCCCCCAGGA 
CTGCCTGGCA TCAAGGGGGA GCGAGGCTAC GCGGGTTCCC CGGGAGAGAA GGGAGAATCG 
GGCCCACCAG GATCTGAAGG CCTCCCAGGG CCCCCAGGCC CAGCGGGTCC CCGAGGAGAG 
CGAGGGCCCC AAGGAAGCTC GGGTGAGAAA GGAGACCAGG GATTCCAAGG GCAACCCGGC 
TTTCCAGGCC CCCCGGGTCC CCCTGGATTC CCAGGCAAAG CTGGAGCACC TGGCCCACCC 
GGCCCCCAAG CGGATAAGGG CAGTGAAGGG ATTCGAGGCC CATCAGGTCT GCCTGGTCCC 
CCTGGACCAC CAGGATCTCC CGGGATTCAG GGCCCCGCCG GTCTGGATGG TCTGGATGGG 
AAGGATGGCA AACCTGGCTT GAGGGGGGAC CCTGGTCCTG CTGGCCCTCC CGGACTCATG 
GGACCCCCGG GCTTCAAGGG AAAACCCGGC CATCCTGGCC TCCCAGGACA GAAGGGCGAC 
TGTGGAAAGC CAGGCCCCCA GGGCAGCAGT GGCCGGCCCG GAGCAGAGGG TGAGCCTGGT 
GCCATGGGAC CCCAGGGAAG ACCCGGCCCC CCTGGCCATG TTGGGCCGCC GGGGCCTCCA 
GGCCAGCCAG GCCCAGCTGG GATCTCTGCA GTGGGCCCAA AAGGAGACCG TGGAGCCACT 
GGAGAAAGGG GCCTTTTAGG TCTTCCAGGC CAACCTGGCC CCCCTGGACA CCCTGGTCCC 
CCGGGTGAAC CCGGCATGGA TGGCGCAGCT GGCAAAGAGG GACCCCCTGG AAAACAGGGA 
CTCTATGGAC CCCCAGGTCC AAAGGGTGAC CCGGGGCCTT CAGGACAGAA GGGCCAGGCA 
GGAGAGAAGG GAAGAGCTGG CATGCCTGGC GGGCCCGGCA AGAGTGGCTC CATGGGACCT 
GCGGGGCCAC CGGGTCCTGC AGGAGAGAGA GGCCACCCTG GATCTCCGGG GCCTGCGGGG 
AGCCCTGGAT TGCCCGGGGT GCCCGGCTCC ATGGGAGACA TGGTGAATTA TGATGAAATC 
AAGAGGTTCA TCAGACAAGA GATCATTAAA ATGTTTGATG AGAGGATGGC TTACTACACC 
TCCAGGATGC AGTTTCCCAT GGAGATGGCA GCAGCTCCAG GGCGACCAGG GCCCCCAGGG 
AAGGATGGTC CCCCAGGCCG GCCAGGCTCC CCGGGATCAC CGGGACTCCC CGGTCAGATT 
GGCAGAGAAG GACGGCAGGG CTTGCCCGGG ATGAGAGGGT TGCCTGGTAC CAAAGGTGAA 
AAGGGAGACA TCGGTGTTGG CATCGCAGGA GAAATTGGTC TTCCTGGCCC CCCAGGTCCC 
CAGGGTCCTC CAGGGTACGG CAAGATGGGT GCGACTGGGC CGATGGGCCA GCAAGGCATT 
CCTGGCATCC CCGGTCCCCC AGGCCCCATA GGCCCGCCGG GCAAGACCGG CCACTGTAAC 
CCCTCTGACT GCTTTGGGGC CATGCCGATG GAGCAACAGT ACCCGCCCAT GAAAAACATG 
AAGGGACCTT TCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462427.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317913.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa16874
Clone ID Related Accession (Same CDS sequence)	XM_005203055.2 , XM_015462427.1
Accession Version	XM_015462427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGCCCCC CAGGTTTTCC TGGTGCTGTT 
GGCTCCCCCG GATTGCCTGG TCTTCAAGGA GAGCGAGGCC TCAGGGGCCT GACGGGAGAG 
AAGGGGGAGC CGGGTCCTCC AGGGCAACCT GGTTACCCAG GCGCCATGGG CCCCCCAGGA 
CTGCCTGGCA TCAAGGGGGA GCGAGGCTAC GCGGGTTCCC CGGGAGAGAA GGGAGAATCG 
GGCCCACCAG GATCTGAAGG CCTCCCAGGG CCCCCAGGCC CAGCGGGTCC CCGAGGAGAG 
CGAGGGCCCC AAGGAAGCTC GGGTGAGAAA GGAGACCAGG GATTCCAAGG GCAACCCGGC 
TTTCCAGGCC CCCCGGGTCC CCCTGGATTC CCAGGCAAAG CTGGAGCACC TGGCCCACCC 
GGCCCCCAAG CGGATAAGGG CAGTGAAGGG ATTCGAGGCC CATCAGGTCT GCCTGGTCCC 
CCTGGACCAC CAGGATCTCC CGGGATTCAG GGCCCCGCCG GTCTGGATGG TCTGGATGGG 
AAGGATGGCA AACCTGGCTT GAGGGGGGAC CCTGGTCCTG CTGGCCCTCC CGGACTCATG 
GGACCCCCGG GCTTCAAGGG AAAACCCGGC CATCCTGGCC TCCCAGGACA GAAGGGCGAC 
TGTGGAAAGC CAGGCCCCCA GGGCAGCAGT GGCCGGCCCG GAGCAGAGGG TGAGCCTGGT 
GCCATGGGAC CCCAGGGAAG ACCCGGCCCC CCTGGCCATG TTGGGCCGCC GGGGCCTCCA 
GGCCAGCCAG GCCCAGCTGG GATCTCTGCA GTGGGCCCAA AAGGAGACCG TGGAGCCACT 
GGAGAAAGGG GCCTTTTAGG TCTTCCAGGC CAACCTGGCC CCCCTGGACA CCCTGGTCCC 
CCGGGTGAAC CCGGCATGGA TGGCGCAGCT GGCAAAGAGG GACCCCCTGG AAAACAGGGA 
CTCTATGGAC CCCCAGGTCC AAAGGGTGAC CCGGGGCCTT CAGGACAGAA GGGCCAGGCA 
GGAGAGAAGG GAAGAGCTGG CATGCCTGGC GGGCCCGGCA AGAGTGGCTC CATGGGACCT 
GCGGGGCCAC CGGGTCCTGC AGGAGAGAGA GGCCACCCTG GATCTCCGGG GCCTGCGGGG 
AGCCCTGGAT TGCCCGGGGT GCCCGGCTCC ATGGGAGACA TGGTGAATTA TGATGAAATC 
AAGAGGTTCA TCAGACAAGA GATCATTAAA ATGTTTGATG AGAGGATGGC TTACTACACC 
TCCAGGATGC AGTTTCCCAT GGAGATGGCA GCAGCTCCAG GGCGACCAGG GCCCCCAGGG 
AAGGATGGTC CCCCAGGCCG GCCAGGCTCC CCGGGATCAC CGGGACTCCC CGGTCAGATT 
GGCAGAGAAG GACGGCAGGG CTTGCCCGGG ATGAGAGGGT TGCCTGGTAC CAAAGGTGAA 
AAGGGAGACA TCGGTGTTGG CATCGCAGGA GAAATTGGTC TTCCTGGCCC CCCAGGTCCC 
CAGGGTCCTC CAGGGTACGG CAAGATGGGT GCGACTGGGC CGATGGGCCA GCAAGGCATT 
CCTGGCATCC CCGGTCCCCC AGGCCCCATA GGCCCGCCGG GCAAGACCGG CCACTGTAAC 
CCCTCTGACT GCTTTGGGGC CATGCCGATG GAGCAACAGT ACCCGCCCAT GAAAAACATG 
AAGGGACCTT TCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317913.1
CDS	206..5083
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462427.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462427.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGCCCCC CAGGTTTTCC TGGTGCTGTT 
GGCTCCCCCG GATTGCCTGG TCTTCAAGGA GAGCGAGGCC TCAGGGGCCT GACGGGAGAG 
AAGGGGGAGC CGGGTCCTCC AGGGCAACCT GGTTACCCAG GCGCCATGGG CCCCCCAGGA 
CTGCCTGGCA TCAAGGGGGA GCGAGGCTAC GCGGGTTCCC CGGGAGAGAA GGGAGAATCG 
GGCCCACCAG GATCTGAAGG CCTCCCAGGG CCCCCAGGCC CAGCGGGTCC CCGAGGAGAG 
CGAGGGCCCC AAGGAAGCTC GGGTGAGAAA GGAGACCAGG GATTCCAAGG GCAACCCGGC 
TTTCCAGGCC CCCCGGGTCC CCCTGGATTC CCAGGCAAAG CTGGAGCACC TGGCCCACCC 
GGCCCCCAAG CGGATAAGGG CAGTGAAGGG ATTCGAGGCC CATCAGGTCT GCCTGGTCCC 
CCTGGACCAC CAGGATCTCC CGGGATTCAG GGCCCCGCCG GTCTGGATGG TCTGGATGGG 
AAGGATGGCA AACCTGGCTT GAGGGGGGAC CCTGGTCCTG CTGGCCCTCC CGGACTCATG 
GGACCCCCGG GCTTCAAGGG AAAACCCGGC CATCCTGGCC TCCCAGGACA GAAGGGCGAC 
TGTGGAAAGC CAGGCCCCCA GGGCAGCAGT GGCCGGCCCG GAGCAGAGGG TGAGCCTGGT 
GCCATGGGAC CCCAGGGAAG ACCCGGCCCC CCTGGCCATG TTGGGCCGCC GGGGCCTCCA 
GGCCAGCCAG GCCCAGCTGG GATCTCTGCA GTGGGCCCAA AAGGAGACCG TGGAGCCACT 
GGAGAAAGGG GCCTTTTAGG TCTTCCAGGC CAACCTGGCC CCCCTGGACA CCCTGGTCCC 
CCGGGTGAAC CCGGCATGGA TGGCGCAGCT GGCAAAGAGG GACCCCCTGG AAAACAGGGA 
CTCTATGGAC CCCCAGGTCC AAAGGGTGAC CCGGGGCCTT CAGGACAGAA GGGCCAGGCA 
GGAGAGAAGG GAAGAGCTGG CATGCCTGGC GGGCCCGGCA AGAGTGGCTC CATGGGACCT 
GCGGGGCCAC CGGGTCCTGC AGGAGAGAGA GGCCACCCTG GATCTCCGGG GCCTGCGGGG 
AGCCCTGGAT TGCCCGGGGT GCCCGGCTCC ATGGGAGACA TGGTGAATTA TGATGAAATC 
AAGAGGTTCA TCAGACAAGA GATCATTAAA ATGTTTGATG AGAGGATGGC TTACTACACC 
TCCAGGATGC AGTTTCCCAT GGAGATGGCA GCAGCTCCAG GGCGACCAGG GCCCCCAGGG 
AAGGATGGTC CCCCAGGCCG GCCAGGCTCC CCGGGATCAC CGGGACTCCC CGGTCAGATT 
GGCAGAGAAG GACGGCAGGG CTTGCCCGGG ATGAGAGGGT TGCCTGGTAC CAAAGGTGAA 
AAGGGAGACA TCGGTGTTGG CATCGCAGGA GAAATTGGTC TTCCTGGCCC CCCAGGTCCC 
CAGGGTCCTC CAGGGTACGG CAAGATGGGT GCGACTGGGC CGATGGGCCA GCAAGGCATT 
CCTGGCATCC CCGGTCCCCC AGGCCCCATA GGCCCGCCGG GCAAGACCGG CCACTGTAAC 
CCCTCTGACT GCTTTGGGGC CATGCCGATG GAGCAACAGT ACCCGCCCAT GAAAAACATG 
AAGGGACCTT TCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203056.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203113.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa16875
Clone ID Related Accession (Same CDS sequence)	XM_005203056.2 , XM_015462428.1
Accession Version	XM_005203056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005203056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGTCTT 
CAAGGAGAGC GAGGCCTCAG GGGCCTGACG GGAGAGAAGG GGGAGCCGGG TCCTCCAGGG 
CAACCTGGTT ACCCAGGCGC CATGGGCCCC CCAGGACTGC CTGGCATCAA GGGGGAGCGA 
GGCTACGCGG GTTCCCCGGG AGAGAAGGGA GAATCGGGCC CACCAGGATC TGAAGGCCTC 
CCAGGGCCCC CAGGCCCAGC GGGTCCCCGA GGAGAGCGAG GGCCCCAAGG AAGCTCGGGT 
GAGAAAGGAG ACCAGGGATT CCAAGGGCAA CCCGGCTTTC CAGGCCCCCC GGGTCCCCCT 
GGATTCCCAG GCAAAGCTGG AGCACCTGGC CCACCCGGCC CCCAAGCGGA TAAGGGCAGT 
GAAGGGATTC GAGGCCCATC AGGTCTGCCT GGTCCCCCTG GACCACCAGG ATCTCCCGGG 
ATTCAGGGCC CCGCCGGTCT GGATGGTCTG GATGGGAAGG ATGGCAAACC TGGCTTGAGG 
GGGGACCCTG GTCCTGCTGG CCCTCCCGGA CTCATGGGAC CCCCGGGCTT CAAGGGAAAA 
CCCGGCCATC CTGGCCTCCC AGGACAGAAG GGCGACTGTG GAAAGCCAGG CCCCCAGGGC 
AGCAGTGGCC GGCCCGGAGC AGAGGGTGAG CCTGGTGCCA TGGGACCCCA GGGAAGACCC 
GGCCCCCCTG GCCATGTTGG GCCGCCGGGG CCTCCAGGCC AGCCAGGCCC AGCTGGGATC 
TCTGCAGTGG GCCCAAAAGG AGACCGTGGA GCCACTGGAG AAAGGGGCCT TTTAGGTCTT 
CCAGGCCAAC CTGGCCCCCC TGGACACCCT GGTCCCCCGG GTGAACCCGG CATGGATGGC 
GCAGCTGGCA AAGAGGGACC CCCTGGAAAA CAGGGACTCT ATGGACCCCC AGGTCCAAAG 
GGTGACCCGG GGCCTTCAGG ACAGAAGGGC CAGGCAGGAG AGAAGGGAAG AGCTGGCATG 
CCTGGCGGGC CCGGCAAGAG TGGCTCCATG GGACCTGCGG GGCCACCGGG TCCTGCAGGA 
GAGAGAGGCC ACCCTGGATC TCCGGGGCCT GCGGGGAGCC CTGGATTGCC CGGGGTGCCC 
GGCTCCATGG GAGACATGGT GAATTATGAT GAAATCAAGA GGTTCATCAG ACAAGAGATC 
ATTAAAATGT TTGATGAGAG GATGGCTTAC TACACCTCCA GGATGCAGTT TCCCATGGAG 
ATGGCAGCAG CTCCAGGGCG ACCAGGGCCC CCAGGGAAGG ATGGTCCCCC AGGCCGGCCA 
GGCTCCCCGG GATCACCGGG ACTCCCCGGT CAGATTGGCA GAGAAGGACG GCAGGGCTTG 
CCCGGGATGA GAGGGTTGCC TGGTACCAAA GGTGAAAAGG GAGACATCGG TGTTGGCATC 
GCAGGAGAAA TTGGTCTTCC TGGCCCCCCA GGTCCCCAGG GTCCTCCAGG GTACGGCAAG 
ATGGGTGCGA CTGGGCCGAT GGGCCAGCAA GGCATTCCTG GCATCCCCGG TCCCCCAGGC 
CCCATAGGCC CGCCGGGCAA GACCGGCCAC TGTAACCCCT CTGACTGCTT TGGGGCCATG 
CCGATGGAGC AACAGTACCC GCCCATGAAA AACATGAAGG GACCTTTCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203113.1
CDS	106..4959
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203056.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203056.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGTCTT 
CAAGGAGAGC GAGGCCTCAG GGGCCTGACG GGAGAGAAGG GGGAGCCGGG TCCTCCAGGG 
CAACCTGGTT ACCCAGGCGC CATGGGCCCC CCAGGACTGC CTGGCATCAA GGGGGAGCGA 
GGCTACGCGG GTTCCCCGGG AGAGAAGGGA GAATCGGGCC CACCAGGATC TGAAGGCCTC 
CCAGGGCCCC CAGGCCCAGC GGGTCCCCGA GGAGAGCGAG GGCCCCAAGG AAGCTCGGGT 
GAGAAAGGAG ACCAGGGATT CCAAGGGCAA CCCGGCTTTC CAGGCCCCCC GGGTCCCCCT 
GGATTCCCAG GCAAAGCTGG AGCACCTGGC CCACCCGGCC CCCAAGCGGA TAAGGGCAGT 
GAAGGGATTC GAGGCCCATC AGGTCTGCCT GGTCCCCCTG GACCACCAGG ATCTCCCGGG 
ATTCAGGGCC CCGCCGGTCT GGATGGTCTG GATGGGAAGG ATGGCAAACC TGGCTTGAGG 
GGGGACCCTG GTCCTGCTGG CCCTCCCGGA CTCATGGGAC CCCCGGGCTT CAAGGGAAAA 
CCCGGCCATC CTGGCCTCCC AGGACAGAAG GGCGACTGTG GAAAGCCAGG CCCCCAGGGC 
AGCAGTGGCC GGCCCGGAGC AGAGGGTGAG CCTGGTGCCA TGGGACCCCA GGGAAGACCC 
GGCCCCCCTG GCCATGTTGG GCCGCCGGGG CCTCCAGGCC AGCCAGGCCC AGCTGGGATC 
TCTGCAGTGG GCCCAAAAGG AGACCGTGGA GCCACTGGAG AAAGGGGCCT TTTAGGTCTT 
CCAGGCCAAC CTGGCCCCCC TGGACACCCT GGTCCCCCGG GTGAACCCGG CATGGATGGC 
GCAGCTGGCA AAGAGGGACC CCCTGGAAAA CAGGGACTCT ATGGACCCCC AGGTCCAAAG 
GGTGACCCGG GGCCTTCAGG ACAGAAGGGC CAGGCAGGAG AGAAGGGAAG AGCTGGCATG 
CCTGGCGGGC CCGGCAAGAG TGGCTCCATG GGACCTGCGG GGCCACCGGG TCCTGCAGGA 
GAGAGAGGCC ACCCTGGATC TCCGGGGCCT GCGGGGAGCC CTGGATTGCC CGGGGTGCCC 
GGCTCCATGG GAGACATGGT GAATTATGAT GAAATCAAGA GGTTCATCAG ACAAGAGATC 
ATTAAAATGT TTGATGAGAG GATGGCTTAC TACACCTCCA GGATGCAGTT TCCCATGGAG 
ATGGCAGCAG CTCCAGGGCG ACCAGGGCCC CCAGGGAAGG ATGGTCCCCC AGGCCGGCCA 
GGCTCCCCGG GATCACCGGG ACTCCCCGGT CAGATTGGCA GAGAAGGACG GCAGGGCTTG 
CCCGGGATGA GAGGGTTGCC TGGTACCAAA GGTGAAAAGG GAGACATCGG TGTTGGCATC 
GCAGGAGAAA TTGGTCTTCC TGGCCCCCCA GGTCCCCAGG GTCCTCCAGG GTACGGCAAG 
ATGGGTGCGA CTGGGCCGAT GGGCCAGCAA GGCATTCCTG GCATCCCCGG TCCCCCAGGC 
CCCATAGGCC CGCCGGGCAA GACCGGCCAC TGTAACCCCT CTGACTGCTT TGGGGCCATG 
CCGATGGAGC AACAGTACCC GCCCATGAAA AACATGAAGG GACCTTTCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462428.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317914.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X4, mRNA.

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CloneID	OBa16875
Clone ID Related Accession (Same CDS sequence)	XM_005203056.2 , XM_015462428.1
Accession Version	XM_015462428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGTCTT 
CAAGGAGAGC GAGGCCTCAG GGGCCTGACG GGAGAGAAGG GGGAGCCGGG TCCTCCAGGG 
CAACCTGGTT ACCCAGGCGC CATGGGCCCC CCAGGACTGC CTGGCATCAA GGGGGAGCGA 
GGCTACGCGG GTTCCCCGGG AGAGAAGGGA GAATCGGGCC CACCAGGATC TGAAGGCCTC 
CCAGGGCCCC CAGGCCCAGC GGGTCCCCGA GGAGAGCGAG GGCCCCAAGG AAGCTCGGGT 
GAGAAAGGAG ACCAGGGATT CCAAGGGCAA CCCGGCTTTC CAGGCCCCCC GGGTCCCCCT 
GGATTCCCAG GCAAAGCTGG AGCACCTGGC CCACCCGGCC CCCAAGCGGA TAAGGGCAGT 
GAAGGGATTC GAGGCCCATC AGGTCTGCCT GGTCCCCCTG GACCACCAGG ATCTCCCGGG 
ATTCAGGGCC CCGCCGGTCT GGATGGTCTG GATGGGAAGG ATGGCAAACC TGGCTTGAGG 
GGGGACCCTG GTCCTGCTGG CCCTCCCGGA CTCATGGGAC CCCCGGGCTT CAAGGGAAAA 
CCCGGCCATC CTGGCCTCCC AGGACAGAAG GGCGACTGTG GAAAGCCAGG CCCCCAGGGC 
AGCAGTGGCC GGCCCGGAGC AGAGGGTGAG CCTGGTGCCA TGGGACCCCA GGGAAGACCC 
GGCCCCCCTG GCCATGTTGG GCCGCCGGGG CCTCCAGGCC AGCCAGGCCC AGCTGGGATC 
TCTGCAGTGG GCCCAAAAGG AGACCGTGGA GCCACTGGAG AAAGGGGCCT TTTAGGTCTT 
CCAGGCCAAC CTGGCCCCCC TGGACACCCT GGTCCCCCGG GTGAACCCGG CATGGATGGC 
GCAGCTGGCA AAGAGGGACC CCCTGGAAAA CAGGGACTCT ATGGACCCCC AGGTCCAAAG 
GGTGACCCGG GGCCTTCAGG ACAGAAGGGC CAGGCAGGAG AGAAGGGAAG AGCTGGCATG 
CCTGGCGGGC CCGGCAAGAG TGGCTCCATG GGACCTGCGG GGCCACCGGG TCCTGCAGGA 
GAGAGAGGCC ACCCTGGATC TCCGGGGCCT GCGGGGAGCC CTGGATTGCC CGGGGTGCCC 
GGCTCCATGG GAGACATGGT GAATTATGAT GAAATCAAGA GGTTCATCAG ACAAGAGATC 
ATTAAAATGT TTGATGAGAG GATGGCTTAC TACACCTCCA GGATGCAGTT TCCCATGGAG 
ATGGCAGCAG CTCCAGGGCG ACCAGGGCCC CCAGGGAAGG ATGGTCCCCC AGGCCGGCCA 
GGCTCCCCGG GATCACCGGG ACTCCCCGGT CAGATTGGCA GAGAAGGACG GCAGGGCTTG 
CCCGGGATGA GAGGGTTGCC TGGTACCAAA GGTGAAAAGG GAGACATCGG TGTTGGCATC 
GCAGGAGAAA TTGGTCTTCC TGGCCCCCCA GGTCCCCAGG GTCCTCCAGG GTACGGCAAG 
ATGGGTGCGA CTGGGCCGAT GGGCCAGCAA GGCATTCCTG GCATCCCCGG TCCCCCAGGC 
CCCATAGGCC CGCCGGGCAA GACCGGCCAC TGTAACCCCT CTGACTGCTT TGGGGCCATG 
CCGATGGAGC AACAGTACCC GCCCATGAAA AACATGAAGG GACCTTTCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317914.1
CDS	207..5060
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462428.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462428.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCCACCTGG TATGAGGGGC TCCCCGGGTC CTCCAGGCCC TATTGGTCTT 
CAAGGAGAGC GAGGCCTCAG GGGCCTGACG GGAGAGAAGG GGGAGCCGGG TCCTCCAGGG 
CAACCTGGTT ACCCAGGCGC CATGGGCCCC CCAGGACTGC CTGGCATCAA GGGGGAGCGA 
GGCTACGCGG GTTCCCCGGG AGAGAAGGGA GAATCGGGCC CACCAGGATC TGAAGGCCTC 
CCAGGGCCCC CAGGCCCAGC GGGTCCCCGA GGAGAGCGAG GGCCCCAAGG AAGCTCGGGT 
GAGAAAGGAG ACCAGGGATT CCAAGGGCAA CCCGGCTTTC CAGGCCCCCC GGGTCCCCCT 
GGATTCCCAG GCAAAGCTGG AGCACCTGGC CCACCCGGCC CCCAAGCGGA TAAGGGCAGT 
GAAGGGATTC GAGGCCCATC AGGTCTGCCT GGTCCCCCTG GACCACCAGG ATCTCCCGGG 
ATTCAGGGCC CCGCCGGTCT GGATGGTCTG GATGGGAAGG ATGGCAAACC TGGCTTGAGG 
GGGGACCCTG GTCCTGCTGG CCCTCCCGGA CTCATGGGAC CCCCGGGCTT CAAGGGAAAA 
CCCGGCCATC CTGGCCTCCC AGGACAGAAG GGCGACTGTG GAAAGCCAGG CCCCCAGGGC 
AGCAGTGGCC GGCCCGGAGC AGAGGGTGAG CCTGGTGCCA TGGGACCCCA GGGAAGACCC 
GGCCCCCCTG GCCATGTTGG GCCGCCGGGG CCTCCAGGCC AGCCAGGCCC AGCTGGGATC 
TCTGCAGTGG GCCCAAAAGG AGACCGTGGA GCCACTGGAG AAAGGGGCCT TTTAGGTCTT 
CCAGGCCAAC CTGGCCCCCC TGGACACCCT GGTCCCCCGG GTGAACCCGG CATGGATGGC 
GCAGCTGGCA AAGAGGGACC CCCTGGAAAA CAGGGACTCT ATGGACCCCC AGGTCCAAAG 
GGTGACCCGG GGCCTTCAGG ACAGAAGGGC CAGGCAGGAG AGAAGGGAAG AGCTGGCATG 
CCTGGCGGGC CCGGCAAGAG TGGCTCCATG GGACCTGCGG GGCCACCGGG TCCTGCAGGA 
GAGAGAGGCC ACCCTGGATC TCCGGGGCCT GCGGGGAGCC CTGGATTGCC CGGGGTGCCC 
GGCTCCATGG GAGACATGGT GAATTATGAT GAAATCAAGA GGTTCATCAG ACAAGAGATC 
ATTAAAATGT TTGATGAGAG GATGGCTTAC TACACCTCCA GGATGCAGTT TCCCATGGAG 
ATGGCAGCAG CTCCAGGGCG ACCAGGGCCC CCAGGGAAGG ATGGTCCCCC AGGCCGGCCA 
GGCTCCCCGG GATCACCGGG ACTCCCCGGT CAGATTGGCA GAGAAGGACG GCAGGGCTTG 
CCCGGGATGA GAGGGTTGCC TGGTACCAAA GGTGAAAAGG GAGACATCGG TGTTGGCATC 
GCAGGAGAAA TTGGTCTTCC TGGCCCCCCA GGTCCCCAGG GTCCTCCAGG GTACGGCAAG 
ATGGGTGCGA CTGGGCCGAT GGGCCAGCAA GGCATTCCTG GCATCCCCGG TCCCCCAGGC 
CCCATAGGCC CGCCGGGCAA GACCGGCCAC TGTAACCCCT CTGACTGCTT TGGGGCCATG 
CCGATGGAGC AACAGTACCC GCCCATGAAA AACATGAAGG GACCTTTCGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_005203057.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203114.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X5, mRNA.

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CloneID	OBa16876
Clone ID Related Accession (Same CDS sequence)	XM_005203057.2 , XM_015462429.1
Accession Version	XM_005203057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005203057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTCCT 
CCGGGTGTTC CTGGACCACC AGGCCCTCCT GGAATGCCTG GGCTGCAGGG GGTGCCCGGG 
CACAACGGTT TGCCAGGACA GCCCGGGCTA ACTGCAGAAC TGGGATCCTT GCCCATTGAA 
CAGCACCTCC TGAAGAGCAT CTGCGGGGAC TGTATCCAGG GCAAGATGGC TCACGAGGTG 
GCCCTCATGG AAAGCGGAGA GAAGGGAGAC CAGGGCATCC CCGGCGTGCC AGGCCTCGAC 
AGTTGCACCC AGTGCTTCAT GGAGCGGGAG CGCCCGAGAG CCGAGGAGGC CCGGGGGGAC 
AACCATGAGG GAGACCCGGG CTGTGCAGGG AGCCCCGGGC TGCCGGGACC TCCGGGACTG 
CCTGGCCAGA GAGGAGAAGA GGGTCCACCT GGTATGAGGG GCTCCCCGGG TCCTCCAGGC 
CCTATTGGCC CCCCAGGTTT TCCTGGTGCT GTTGGCTCCC CCGGATTGCC TGGTCTTCAA 
GGAGAGCGAG GCCTCAGGGG CCTGACGGGA GAGAAGGGGG AGCCGGGTCC TCCAGGGCAA 
CCTGGTTACC CAGGCGCCAT GGGCCCCCCA GGACTGCCTG GCATCAAGGG GGAGCGAGGC 
TACGCGGGTT CCCCGGGAGA GAAGGGAGAA TCGGGCCCAC CAGGATCTGA AGGCCTCCCA 
GGGCCCCCAG GCCCAGCGGG TCCCCGAGGA GAGCGAGGGC CCCAAGGAAG CTCGGGTGAG 
AAAGGAGACC AGGGATTCCA AGGGCAACCC GGCTTTCCAG GCCCCCCGGG TCCCCCTGGA 
TTCCCAGGCA AAGCTGGAGC ACCTGGCCCA CCCGGCCCCC AAGCGGATAA GGGCAGTGAA 
GGGATTCGAG GCCCATCAGG TCTGCCTGGT CCCCCTGGAC CACCAGGATC TCCCGGGATT 
CAGGGCCCCG CCGGTCTGGA TGGTCTGGAT GGGAAGGATG GCAAACCTGG CTTGAGGGGG 
GACCCTGGTC CTGCTGGCCC TCCCGGACTC ATGGGACCCC CGGGCTTCAA GGGAAAACCC 
GGCCATCCTG GCCTCCCAGG ACAGAAGGGC GACTGTGGAA AGCCAGGCCC CCAGGGCAGC 
AGTGGCCGGC CCGGAGCAGA GGGTGAGCCT GGTGCCATGG GACCCCAGGG AAGACCCGGC 
CCCCCTGGCC ATGTTGGGCC GCCGGGGCCT CCAGGCCAGC CAGGCCCAGC TGGGATCTCT 
GCAGTGGGCC CAAAAGGAGA CCGTGGAGCC ACTGGAGAAA GGGGCCTTTT AGGTCTTCCA 
GGCCAACCTG GCCCCCCTGG ACACCCTGGT CCCCCGGGTG AACCCGGCAT GGATGGCGCA 
GCTGGCAAAG AGGGACCCCC TGGAAAACAG GGACTCTATG GACCCCCAGG TCCAAAGGGT 
GACCCGGGGC CTTCAGGACA GAAGGGCCAG GCAGGAGAGA AGGGAAGAGC TGGCATGCCT 
GGCGGGCCCG GCAAGAGTGG CTCCATGGGA CCTGCGGGGC CACCGGGTCC TGCAGGAGAG 
AGAGGCCACC CTGGATCTCC GGGGCCTGCG GGGAGCCCTG GATTGCCCGG GGTGCCCGGC 
TCCATGGGAG ACATGGTGAA TTATGATGAA ATCAAGAGGT TCATCAGACA AGAGATCATT 
AAAATGTTTG ATGAGAGGAT GGCTTACTAC ACCTCCAGGA TGCAGTTTCC CATGGAGATG 
GCAGCAGCTC CAGGGCGACC AGGGCCCCCA GGGAAGGATG GTCCCCCAGG CCGGCCAGGC 
TCCCCGGGAT CACCGGGACT CCCCGGTCAG ATTGGCAGAG AAGGACGGCA GGGCTTGCCC 
GGGATGAGAG GGTTGCCTGG TACCAAAGGT GAAAAGGGAG ACATCGGTGT TGGCATCGCA 
GGAGAAATTG GTCTTCCTGG CCCCCCAGGT CCCCAGGGTC CTCCAGGGTA CGGCAAGATG 
GGTGCGACTG GGCCGATGGG CCAGCAAGGC ATTCCTGGCA TCCCCGGTCC CCCAGGCCCC 
ATAGGCCCGC CGGGCAAGAC CGGCCACTGT AACCCCTCTG ACTGCTTTGG GGCCATGCCG 
ATGGAGCAAC AGTACCCGCC CATGAAAAAC ATGAAGGGAC CTTTCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203114.1
CDS	106..4956
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_005203057.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203057.2

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTCCT 
CCGGGTGTTC CTGGACCACC AGGCCCTCCT GGAATGCCTG GGCTGCAGGG GGTGCCCGGG 
CACAACGGTT TGCCAGGACA GCCCGGGCTA ACTGCAGAAC TGGGATCCTT GCCCATTGAA 
CAGCACCTCC TGAAGAGCAT CTGCGGGGAC TGTATCCAGG GCAAGATGGC TCACGAGGTG 
GCCCTCATGG AAAGCGGAGA GAAGGGAGAC CAGGGCATCC CCGGCGTGCC AGGCCTCGAC 
AGTTGCACCC AGTGCTTCAT GGAGCGGGAG CGCCCGAGAG CCGAGGAGGC CCGGGGGGAC 
AACCATGAGG GAGACCCGGG CTGTGCAGGG AGCCCCGGGC TGCCGGGACC TCCGGGACTG 
CCTGGCCAGA GAGGAGAAGA GGGTCCACCT GGTATGAGGG GCTCCCCGGG TCCTCCAGGC 
CCTATTGGCC CCCCAGGTTT TCCTGGTGCT GTTGGCTCCC CCGGATTGCC TGGTCTTCAA 
GGAGAGCGAG GCCTCAGGGG CCTGACGGGA GAGAAGGGGG AGCCGGGTCC TCCAGGGCAA 
CCTGGTTACC CAGGCGCCAT GGGCCCCCCA GGACTGCCTG GCATCAAGGG GGAGCGAGGC 
TACGCGGGTT CCCCGGGAGA GAAGGGAGAA TCGGGCCCAC CAGGATCTGA AGGCCTCCCA 
GGGCCCCCAG GCCCAGCGGG TCCCCGAGGA GAGCGAGGGC CCCAAGGAAG CTCGGGTGAG 
AAAGGAGACC AGGGATTCCA AGGGCAACCC GGCTTTCCAG GCCCCCCGGG TCCCCCTGGA 
TTCCCAGGCA AAGCTGGAGC ACCTGGCCCA CCCGGCCCCC AAGCGGATAA GGGCAGTGAA 
GGGATTCGAG GCCCATCAGG TCTGCCTGGT CCCCCTGGAC CACCAGGATC TCCCGGGATT 
CAGGGCCCCG CCGGTCTGGA TGGTCTGGAT GGGAAGGATG GCAAACCTGG CTTGAGGGGG 
GACCCTGGTC CTGCTGGCCC TCCCGGACTC ATGGGACCCC CGGGCTTCAA GGGAAAACCC 
GGCCATCCTG GCCTCCCAGG ACAGAAGGGC GACTGTGGAA AGCCAGGCCC CCAGGGCAGC 
AGTGGCCGGC CCGGAGCAGA GGGTGAGCCT GGTGCCATGG GACCCCAGGG AAGACCCGGC 
CCCCCTGGCC ATGTTGGGCC GCCGGGGCCT CCAGGCCAGC CAGGCCCAGC TGGGATCTCT 
GCAGTGGGCC CAAAAGGAGA CCGTGGAGCC ACTGGAGAAA GGGGCCTTTT AGGTCTTCCA 
GGCCAACCTG GCCCCCCTGG ACACCCTGGT CCCCCGGGTG AACCCGGCAT GGATGGCGCA 
GCTGGCAAAG AGGGACCCCC TGGAAAACAG GGACTCTATG GACCCCCAGG TCCAAAGGGT 
GACCCGGGGC CTTCAGGACA GAAGGGCCAG GCAGGAGAGA AGGGAAGAGC TGGCATGCCT 
GGCGGGCCCG GCAAGAGTGG CTCCATGGGA CCTGCGGGGC CACCGGGTCC TGCAGGAGAG 
AGAGGCCACC CTGGATCTCC GGGGCCTGCG GGGAGCCCTG GATTGCCCGG GGTGCCCGGC 
TCCATGGGAG ACATGGTGAA TTATGATGAA ATCAAGAGGT TCATCAGACA AGAGATCATT 
AAAATGTTTG ATGAGAGGAT GGCTTACTAC ACCTCCAGGA TGCAGTTTCC CATGGAGATG 
GCAGCAGCTC CAGGGCGACC AGGGCCCCCA GGGAAGGATG GTCCCCCAGG CCGGCCAGGC 
TCCCCGGGAT CACCGGGACT CCCCGGTCAG ATTGGCAGAG AAGGACGGCA GGGCTTGCCC 
GGGATGAGAG GGTTGCCTGG TACCAAAGGT GAAAAGGGAG ACATCGGTGT TGGCATCGCA 
GGAGAAATTG GTCTTCCTGG CCCCCCAGGT CCCCAGGGTC CTCCAGGGTA CGGCAAGATG 
GGTGCGACTG GGCCGATGGG CCAGCAAGGC ATTCCTGGCA TCCCCGGTCC CCCAGGCCCC 
ATAGGCCCGC CGGGCAAGAC CGGCCACTGT AACCCCTCTG ACTGCTTTGG GGCCATGCCG 
ATGGAGCAAC AGTACCCGCC CATGAAAAAC ATGAAGGGAC CTTTCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462429.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317915.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X5, mRNA.

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CloneID	OBa16876
Clone ID Related Accession (Same CDS sequence)	XM_005203057.2 , XM_015462429.1
Accession Version	XM_015462429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTCCT 
CCGGGTGTTC CTGGACCACC AGGCCCTCCT GGAATGCCTG GGCTGCAGGG GGTGCCCGGG 
CACAACGGTT TGCCAGGACA GCCCGGGCTA ACTGCAGAAC TGGGATCCTT GCCCATTGAA 
CAGCACCTCC TGAAGAGCAT CTGCGGGGAC TGTATCCAGG GCAAGATGGC TCACGAGGTG 
GCCCTCATGG AAAGCGGAGA GAAGGGAGAC CAGGGCATCC CCGGCGTGCC AGGCCTCGAC 
AGTTGCACCC AGTGCTTCAT GGAGCGGGAG CGCCCGAGAG CCGAGGAGGC CCGGGGGGAC 
AACCATGAGG GAGACCCGGG CTGTGCAGGG AGCCCCGGGC TGCCGGGACC TCCGGGACTG 
CCTGGCCAGA GAGGAGAAGA GGGTCCACCT GGTATGAGGG GCTCCCCGGG TCCTCCAGGC 
CCTATTGGCC CCCCAGGTTT TCCTGGTGCT GTTGGCTCCC CCGGATTGCC TGGTCTTCAA 
GGAGAGCGAG GCCTCAGGGG CCTGACGGGA GAGAAGGGGG AGCCGGGTCC TCCAGGGCAA 
CCTGGTTACC CAGGCGCCAT GGGCCCCCCA GGACTGCCTG GCATCAAGGG GGAGCGAGGC 
TACGCGGGTT CCCCGGGAGA GAAGGGAGAA TCGGGCCCAC CAGGATCTGA AGGCCTCCCA 
GGGCCCCCAG GCCCAGCGGG TCCCCGAGGA GAGCGAGGGC CCCAAGGAAG CTCGGGTGAG 
AAAGGAGACC AGGGATTCCA AGGGCAACCC GGCTTTCCAG GCCCCCCGGG TCCCCCTGGA 
TTCCCAGGCA AAGCTGGAGC ACCTGGCCCA CCCGGCCCCC AAGCGGATAA GGGCAGTGAA 
GGGATTCGAG GCCCATCAGG TCTGCCTGGT CCCCCTGGAC CACCAGGATC TCCCGGGATT 
CAGGGCCCCG CCGGTCTGGA TGGTCTGGAT GGGAAGGATG GCAAACCTGG CTTGAGGGGG 
GACCCTGGTC CTGCTGGCCC TCCCGGACTC ATGGGACCCC CGGGCTTCAA GGGAAAACCC 
GGCCATCCTG GCCTCCCAGG ACAGAAGGGC GACTGTGGAA AGCCAGGCCC CCAGGGCAGC 
AGTGGCCGGC CCGGAGCAGA GGGTGAGCCT GGTGCCATGG GACCCCAGGG AAGACCCGGC 
CCCCCTGGCC ATGTTGGGCC GCCGGGGCCT CCAGGCCAGC CAGGCCCAGC TGGGATCTCT 
GCAGTGGGCC CAAAAGGAGA CCGTGGAGCC ACTGGAGAAA GGGGCCTTTT AGGTCTTCCA 
GGCCAACCTG GCCCCCCTGG ACACCCTGGT CCCCCGGGTG AACCCGGCAT GGATGGCGCA 
GCTGGCAAAG AGGGACCCCC TGGAAAACAG GGACTCTATG GACCCCCAGG TCCAAAGGGT 
GACCCGGGGC CTTCAGGACA GAAGGGCCAG GCAGGAGAGA AGGGAAGAGC TGGCATGCCT 
GGCGGGCCCG GCAAGAGTGG CTCCATGGGA CCTGCGGGGC CACCGGGTCC TGCAGGAGAG 
AGAGGCCACC CTGGATCTCC GGGGCCTGCG GGGAGCCCTG GATTGCCCGG GGTGCCCGGC 
TCCATGGGAG ACATGGTGAA TTATGATGAA ATCAAGAGGT TCATCAGACA AGAGATCATT 
AAAATGTTTG ATGAGAGGAT GGCTTACTAC ACCTCCAGGA TGCAGTTTCC CATGGAGATG 
GCAGCAGCTC CAGGGCGACC AGGGCCCCCA GGGAAGGATG GTCCCCCAGG CCGGCCAGGC 
TCCCCGGGAT CACCGGGACT CCCCGGTCAG ATTGGCAGAG AAGGACGGCA GGGCTTGCCC 
GGGATGAGAG GGTTGCCTGG TACCAAAGGT GAAAAGGGAG ACATCGGTGT TGGCATCGCA 
GGAGAAATTG GTCTTCCTGG CCCCCCAGGT CCCCAGGGTC CTCCAGGGTA CGGCAAGATG 
GGTGCGACTG GGCCGATGGG CCAGCAAGGC ATTCCTGGCA TCCCCGGTCC CCCAGGCCCC 
ATAGGCCCGC CGGGCAAGAC CGGCCACTGT AACCCCTCTG ACTGCTTTGG GGCCATGCCG 
ATGGAGCAAC AGTACCCGCC CATGAAAAAC ATGAAGGGAC CTTTCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317915.1
CDS	207..5057
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462429.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462429.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTCCT 
CCGGGTGTTC CTGGACCACC AGGCCCTCCT GGAATGCCTG GGCTGCAGGG GGTGCCCGGG 
CACAACGGTT TGCCAGGACA GCCCGGGCTA ACTGCAGAAC TGGGATCCTT GCCCATTGAA 
CAGCACCTCC TGAAGAGCAT CTGCGGGGAC TGTATCCAGG GCAAGATGGC TCACGAGGTG 
GCCCTCATGG AAAGCGGAGA GAAGGGAGAC CAGGGCATCC CCGGCGTGCC AGGCCTCGAC 
AGTTGCACCC AGTGCTTCAT GGAGCGGGAG CGCCCGAGAG CCGAGGAGGC CCGGGGGGAC 
AACCATGAGG GAGACCCGGG CTGTGCAGGG AGCCCCGGGC TGCCGGGACC TCCGGGACTG 
CCTGGCCAGA GAGGAGAAGA GGGTCCACCT GGTATGAGGG GCTCCCCGGG TCCTCCAGGC 
CCTATTGGCC CCCCAGGTTT TCCTGGTGCT GTTGGCTCCC CCGGATTGCC TGGTCTTCAA 
GGAGAGCGAG GCCTCAGGGG CCTGACGGGA GAGAAGGGGG AGCCGGGTCC TCCAGGGCAA 
CCTGGTTACC CAGGCGCCAT GGGCCCCCCA GGACTGCCTG GCATCAAGGG GGAGCGAGGC 
TACGCGGGTT CCCCGGGAGA GAAGGGAGAA TCGGGCCCAC CAGGATCTGA AGGCCTCCCA 
GGGCCCCCAG GCCCAGCGGG TCCCCGAGGA GAGCGAGGGC CCCAAGGAAG CTCGGGTGAG 
AAAGGAGACC AGGGATTCCA AGGGCAACCC GGCTTTCCAG GCCCCCCGGG TCCCCCTGGA 
TTCCCAGGCA AAGCTGGAGC ACCTGGCCCA CCCGGCCCCC AAGCGGATAA GGGCAGTGAA 
GGGATTCGAG GCCCATCAGG TCTGCCTGGT CCCCCTGGAC CACCAGGATC TCCCGGGATT 
CAGGGCCCCG CCGGTCTGGA TGGTCTGGAT GGGAAGGATG GCAAACCTGG CTTGAGGGGG 
GACCCTGGTC CTGCTGGCCC TCCCGGACTC ATGGGACCCC CGGGCTTCAA GGGAAAACCC 
GGCCATCCTG GCCTCCCAGG ACAGAAGGGC GACTGTGGAA AGCCAGGCCC CCAGGGCAGC 
AGTGGCCGGC CCGGAGCAGA GGGTGAGCCT GGTGCCATGG GACCCCAGGG AAGACCCGGC 
CCCCCTGGCC ATGTTGGGCC GCCGGGGCCT CCAGGCCAGC CAGGCCCAGC TGGGATCTCT 
GCAGTGGGCC CAAAAGGAGA CCGTGGAGCC ACTGGAGAAA GGGGCCTTTT AGGTCTTCCA 
GGCCAACCTG GCCCCCCTGG ACACCCTGGT CCCCCGGGTG AACCCGGCAT GGATGGCGCA 
GCTGGCAAAG AGGGACCCCC TGGAAAACAG GGACTCTATG GACCCCCAGG TCCAAAGGGT 
GACCCGGGGC CTTCAGGACA GAAGGGCCAG GCAGGAGAGA AGGGAAGAGC TGGCATGCCT 
GGCGGGCCCG GCAAGAGTGG CTCCATGGGA CCTGCGGGGC CACCGGGTCC TGCAGGAGAG 
AGAGGCCACC CTGGATCTCC GGGGCCTGCG GGGAGCCCTG GATTGCCCGG GGTGCCCGGC 
TCCATGGGAG ACATGGTGAA TTATGATGAA ATCAAGAGGT TCATCAGACA AGAGATCATT 
AAAATGTTTG ATGAGAGGAT GGCTTACTAC ACCTCCAGGA TGCAGTTTCC CATGGAGATG 
GCAGCAGCTC CAGGGCGACC AGGGCCCCCA GGGAAGGATG GTCCCCCAGG CCGGCCAGGC 
TCCCCGGGAT CACCGGGACT CCCCGGTCAG ATTGGCAGAG AAGGACGGCA GGGCTTGCCC 
GGGATGAGAG GGTTGCCTGG TACCAAAGGT GAAAAGGGAG ACATCGGTGT TGGCATCGCA 
GGAGAAATTG GTCTTCCTGG CCCCCCAGGT CCCCAGGGTC CTCCAGGGTA CGGCAAGATG 
GGTGCGACTG GGCCGATGGG CCAGCAAGGC ATTCCTGGCA TCCCCGGTCC CCCAGGCCCC 
ATAGGCCCGC CGGGCAAGAC CGGCCACTGT AACCCCTCTG ACTGCTTTGG GGCCATGCCG 
ATGGAGCAAC AGTACCCGCC CATGAAAAAC ATGAAGGGAC CTTTCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_010802506.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800808.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X7, mRNA.

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CloneID	OBa46726
Clone ID Related Accession (Same CDS sequence)	XM_010802506.1 , XM_015462431.1
Accession Version	XM_010802506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGTCTTC AAGGAGAGCG AGGCCTCAGG 
GGCCTGACGG GAGAGAAGGG GGAGCCGGGT CCTCCAGGGC AACCTGGTTA CCCAGGCGCC 
ATGGGCCCCC CAGGACTGCC TGGCATCAAG GGGGAGCGAG GCTACGCGGG TTCCCCGGGA 
GAGAAGGGAG AATCGGGCCC ACCAGGATCT GAAGGCCTCC CAGGGCCCCC AGGCCCAGCG 
GGTCCCCGAG GAGAGCGAGG GCCCCAAGGA AGCTCGGGTG AGAAAGGAGA CCAGGGATTC 
CAAGGGCAAC CCGGCTTTCC AGGCCCCCCG GGTCCCCCTG GATTCCCAGG CAAAGCTGGA 
GCACCTGGCC CACCCGGCCC CCAAGCGGAT AAGGGCAGTG AAGGGATTCG AGGCCCATCA 
GGTCTGCCTG GTCCCCCTGG ACCACCAGGA TCTCCCGGGA TTCAGGGCCC CGCCGGTCTG 
GATGGTCTGG ATGGGAAGGA TGGCAAACCT GGCTTGAGGG GGGACCCTGG TCCTGCTGGC 
CCTCCCGGAC TCATGGGACC CCCGGGCTTC AAGGGAAAAC CCGGCCATCC TGGCCTCCCA 
GGACAGAAGG GCGACTGTGG AAAGCCAGGC CCCCAGGGCA GCAGTGGCCG GCCCGGAGCA 
GAGGGTGAGC CTGGTGCCAT GGGACCCCAG GGAAGACCCG GCCCCCCTGG CCATGTTGGG 
CCGCCGGGGC CTCCAGGCCA GCCAGGCCCA GCTGGGATCT CTGCAGTGGG CCCAAAAGGA 
GACCGTGGAG CCACTGGAGA AAGGGGCCTT TTAGGTCTTC CAGGCCAACC TGGCCCCCCT 
GGACACCCTG GTCCCCCGGG TGAACCCGGC ATGGATGGCG CAGCTGGCAA AGAGGGACCC 
CCTGGAAAAC AGGGACTCTA TGGACCCCCA GGTCCAAAGG GTGACCCGGG GCCTTCAGGA 
CAGAAGGGCC AGGCAGGAGA GAAGGGAAGA GCTGGCATGC CTGGCGGGCC CGGCAAGAGT 
GGCTCCATGG GACCTGCGGG GCCACCGGGT CCTGCAGGAG AGAGAGGCCA CCCTGGATCT 
CCGGGGCCTG CGGGGAGCCC TGGATTGCCC GGGGTGCCCG GCTCCATGGG AGACATGGTG 
AATTATGATG AAATCAAGAG GTTCATCAGA CAAGAGATCA TTAAAATGTT TGATGAGAGG 
ATGGCTTACT ACACCTCCAG GATGCAGTTT CCCATGGAGA TGGCAGCAGC TCCAGGGCGA 
CCAGGGCCCC CAGGGAAGGA TGGTCCCCCA GGCCGGCCAG GCTCCCCGGG ATCACCGGGA 
CTCCCCGGTC AGATTGGCAG AGAAGGACGG CAGGGCTTGC CCGGGATGAG AGGGTTGCCT 
GGTACCAAAG GTGAAAAGGG AGACATCGGT GTTGGCATCG CAGGAGAAAT TGGTCTTCCT 
GGCCCCCCAG GTCCCCAGGG TCCTCCAGGG TACGGCAAGA TGGGTGCGAC TGGGCCGATG 
GGCCAGCAAG GCATTCCTGG CATCCCCGGT CCCCCAGGCC CCATAGGCCC GCCGGGCAAG 
ACCGGCCACT GTAACCCCTC TGACTGCTTT GGGGCCATGC CGATGGAGCA ACAGTACCCG 
CCCATGAAAA ACATGAAGGG ACCTTTCGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800808.1
CDS	106..4938
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_010802506.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802506.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGTCTTC AAGGAGAGCG AGGCCTCAGG 
GGCCTGACGG GAGAGAAGGG GGAGCCGGGT CCTCCAGGGC AACCTGGTTA CCCAGGCGCC 
ATGGGCCCCC CAGGACTGCC TGGCATCAAG GGGGAGCGAG GCTACGCGGG TTCCCCGGGA 
GAGAAGGGAG AATCGGGCCC ACCAGGATCT GAAGGCCTCC CAGGGCCCCC AGGCCCAGCG 
GGTCCCCGAG GAGAGCGAGG GCCCCAAGGA AGCTCGGGTG AGAAAGGAGA CCAGGGATTC 
CAAGGGCAAC CCGGCTTTCC AGGCCCCCCG GGTCCCCCTG GATTCCCAGG CAAAGCTGGA 
GCACCTGGCC CACCCGGCCC CCAAGCGGAT AAGGGCAGTG AAGGGATTCG AGGCCCATCA 
GGTCTGCCTG GTCCCCCTGG ACCACCAGGA TCTCCCGGGA TTCAGGGCCC CGCCGGTCTG 
GATGGTCTGG ATGGGAAGGA TGGCAAACCT GGCTTGAGGG GGGACCCTGG TCCTGCTGGC 
CCTCCCGGAC TCATGGGACC CCCGGGCTTC AAGGGAAAAC CCGGCCATCC TGGCCTCCCA 
GGACAGAAGG GCGACTGTGG AAAGCCAGGC CCCCAGGGCA GCAGTGGCCG GCCCGGAGCA 
GAGGGTGAGC CTGGTGCCAT GGGACCCCAG GGAAGACCCG GCCCCCCTGG CCATGTTGGG 
CCGCCGGGGC CTCCAGGCCA GCCAGGCCCA GCTGGGATCT CTGCAGTGGG CCCAAAAGGA 
GACCGTGGAG CCACTGGAGA AAGGGGCCTT TTAGGTCTTC CAGGCCAACC TGGCCCCCCT 
GGACACCCTG GTCCCCCGGG TGAACCCGGC ATGGATGGCG CAGCTGGCAA AGAGGGACCC 
CCTGGAAAAC AGGGACTCTA TGGACCCCCA GGTCCAAAGG GTGACCCGGG GCCTTCAGGA 
CAGAAGGGCC AGGCAGGAGA GAAGGGAAGA GCTGGCATGC CTGGCGGGCC CGGCAAGAGT 
GGCTCCATGG GACCTGCGGG GCCACCGGGT CCTGCAGGAG AGAGAGGCCA CCCTGGATCT 
CCGGGGCCTG CGGGGAGCCC TGGATTGCCC GGGGTGCCCG GCTCCATGGG AGACATGGTG 
AATTATGATG AAATCAAGAG GTTCATCAGA CAAGAGATCA TTAAAATGTT TGATGAGAGG 
ATGGCTTACT ACACCTCCAG GATGCAGTTT CCCATGGAGA TGGCAGCAGC TCCAGGGCGA 
CCAGGGCCCC CAGGGAAGGA TGGTCCCCCA GGCCGGCCAG GCTCCCCGGG ATCACCGGGA 
CTCCCCGGTC AGATTGGCAG AGAAGGACGG CAGGGCTTGC CCGGGATGAG AGGGTTGCCT 
GGTACCAAAG GTGAAAAGGG AGACATCGGT GTTGGCATCG CAGGAGAAAT TGGTCTTCCT 
GGCCCCCCAG GTCCCCAGGG TCCTCCAGGG TACGGCAAGA TGGGTGCGAC TGGGCCGATG 
GGCCAGCAAG GCATTCCTGG CATCCCCGGT CCCCCAGGCC CCATAGGCCC GCCGGGCAAG 
ACCGGCCACT GTAACCCCTC TGACTGCTTT GGGGCCATGC CGATGGAGCA ACAGTACCCG 
CCCATGAAAA ACATGAAGGG ACCTTTCGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462431.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317917.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X7, mRNA.

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CloneID	OBa46726
Clone ID Related Accession (Same CDS sequence)	XM_010802506.1 , XM_015462431.1
Accession Version	XM_015462431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGTCTTC AAGGAGAGCG AGGCCTCAGG 
GGCCTGACGG GAGAGAAGGG GGAGCCGGGT CCTCCAGGGC AACCTGGTTA CCCAGGCGCC 
ATGGGCCCCC CAGGACTGCC TGGCATCAAG GGGGAGCGAG GCTACGCGGG TTCCCCGGGA 
GAGAAGGGAG AATCGGGCCC ACCAGGATCT GAAGGCCTCC CAGGGCCCCC AGGCCCAGCG 
GGTCCCCGAG GAGAGCGAGG GCCCCAAGGA AGCTCGGGTG AGAAAGGAGA CCAGGGATTC 
CAAGGGCAAC CCGGCTTTCC AGGCCCCCCG GGTCCCCCTG GATTCCCAGG CAAAGCTGGA 
GCACCTGGCC CACCCGGCCC CCAAGCGGAT AAGGGCAGTG AAGGGATTCG AGGCCCATCA 
GGTCTGCCTG GTCCCCCTGG ACCACCAGGA TCTCCCGGGA TTCAGGGCCC CGCCGGTCTG 
GATGGTCTGG ATGGGAAGGA TGGCAAACCT GGCTTGAGGG GGGACCCTGG TCCTGCTGGC 
CCTCCCGGAC TCATGGGACC CCCGGGCTTC AAGGGAAAAC CCGGCCATCC TGGCCTCCCA 
GGACAGAAGG GCGACTGTGG AAAGCCAGGC CCCCAGGGCA GCAGTGGCCG GCCCGGAGCA 
GAGGGTGAGC CTGGTGCCAT GGGACCCCAG GGAAGACCCG GCCCCCCTGG CCATGTTGGG 
CCGCCGGGGC CTCCAGGCCA GCCAGGCCCA GCTGGGATCT CTGCAGTGGG CCCAAAAGGA 
GACCGTGGAG CCACTGGAGA AAGGGGCCTT TTAGGTCTTC CAGGCCAACC TGGCCCCCCT 
GGACACCCTG GTCCCCCGGG TGAACCCGGC ATGGATGGCG CAGCTGGCAA AGAGGGACCC 
CCTGGAAAAC AGGGACTCTA TGGACCCCCA GGTCCAAAGG GTGACCCGGG GCCTTCAGGA 
CAGAAGGGCC AGGCAGGAGA GAAGGGAAGA GCTGGCATGC CTGGCGGGCC CGGCAAGAGT 
GGCTCCATGG GACCTGCGGG GCCACCGGGT CCTGCAGGAG AGAGAGGCCA CCCTGGATCT 
CCGGGGCCTG CGGGGAGCCC TGGATTGCCC GGGGTGCCCG GCTCCATGGG AGACATGGTG 
AATTATGATG AAATCAAGAG GTTCATCAGA CAAGAGATCA TTAAAATGTT TGATGAGAGG 
ATGGCTTACT ACACCTCCAG GATGCAGTTT CCCATGGAGA TGGCAGCAGC TCCAGGGCGA 
CCAGGGCCCC CAGGGAAGGA TGGTCCCCCA GGCCGGCCAG GCTCCCCGGG ATCACCGGGA 
CTCCCCGGTC AGATTGGCAG AGAAGGACGG CAGGGCTTGC CCGGGATGAG AGGGTTGCCT 
GGTACCAAAG GTGAAAAGGG AGACATCGGT GTTGGCATCG CAGGAGAAAT TGGTCTTCCT 
GGCCCCCCAG GTCCCCAGGG TCCTCCAGGG TACGGCAAGA TGGGTGCGAC TGGGCCGATG 
GGCCAGCAAG GCATTCCTGG CATCCCCGGT CCCCCAGGCC CCATAGGCCC GCCGGGCAAG 
ACCGGCCACT GTAACCCCTC TGACTGCTTT GGGGCCATGC CGATGGAGCA ACAGTACCCG 
CCCATGAAAA ACATGAAGGG ACCTTTCGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317917.1
CDS	206..5038
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462431.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462431.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGTG TCCCCCAGAG ACCTCCAAGG CCCGCCGGGA CACCCAGAAC 
AATGAGCTCA TCGAGATCAA TCCACAGACT GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
CTGGAGGAGC CCCAGAACAG CAAGGTGGAT GCCCAGCTGT CGGGAAGAAT CAGCCAGAAG 
CCAGAAAGAG GAGCAAAGGC CCACCAGGAG ACAGCAGCCG ATGAGTGCCC ACCCTGTGTC 
CATGGTGCCC AGGAGAGCAA CGTCACAATT GGTCCTTCTG GCCCCCAGGG AGGGAAAGGT 
GAGCGGGGCC TGCCTGGTCC ATCAGGACCC AAGGGAGAGA AGGGAGCCCG GGGCAATGAC 
TGTGTTAGAA TCTCCCCGGA TGCCCCGCTT CAGTGTGCAG AAGGCCTGAA GGGAGAGAAG 
GGAGAGTCGG GAGCCTTGGG ACCCTCAGGA CTCCCCGGCT CCACAGGCCA GAAGGGCCAG 
AAAGGCGAGA AGGGAGACGG AGGCGTGAAG GGCTCGCCGG GAAAGCCAGG CCGAGATGGC 
CGGCCAGGAG AAGTCTGTGT CATAGGGCCC AAAGGGCAGA AGGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GGCTGGCAGG AGAGCCCGGG CCCCCAGGCC TCCCTGGGCC CCCTGGCATA 
GGACTTCCGG GGACCCCGGG GGACCCAGGT GGCCCTCCAG GCCCCAAGGG AGACAAGGGC 
AACTCCGGGG TGCCTGGAAA GGAAGGTCCT GGCGGGAAAC CTGGCAAACC AGGGGTGCCC 
GGGCTGAAGG GAGAGAAGGG TGACCCCTGT GAAGTGTGCC CAACACTACC TGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGACCCAAGG GAGAACCAGG TGATCCTGCA 
CCAGCCAGGG AGAGTCTGGG AGCTGTTGGA CAAAAAGGTG ATCGGGGAGA CCCTGGCATC 
CAAGGCCTCA AAGGAGAGAA GGGGGAGTCC TGCTCGTCCT GCAGCTTTGC CGTAGCGGCC 
CAGCATCTCC AGCCTTCTGC AGGGGCCAAA GGCGATGTGG GCCCTCCTGG CCTGGGTTTG 
CCAGGTCTTC CGGGGAAAGC TGGGGCCCCA GGGCCAACAG GGCTGAAAGG AGAAAAGGGT 
AATTTCGGGG AGGTAGGGCC AGCCGGCAAT CCGGGGCCGC CAGGGCCTGC GGGACCCACA 
GGCATCAAAG GGGAGAAGGG GGAGCCTTGT GAGCCATGCA GAGCCCTGAC CGAGCATCAG 
GATGAAGACA GCCACGTGGT GGCCTTGCCG GGGCCCCGCG GAGAGAAGGG GGAGCCCGGG 
CCTCCAGGTT TCGGTCTGCC AGGAAAACAG GGCAAGGCTG GAGAGCGTGG ACTGAAGGGG 
CAAAAGGGTG ATGCTGGGAA TCCTGGAGAC CCTGGAACAC CAGGCATCAC GGGGCGGCCA 
GGACTGTCGG GAGAGCCAGG GGTTCGGGGC CCTGTGGGGC CAAAAGGAGA AAAGGGTGAT 
GGCTGCACCG CCTGCCCCAG CACGCAGGGG GCGCTGACAG ACAGAGCGGG GCCGCCTGGG 
AAGCCGGGCC CGAAAGGAGA TCCTGGGCCA GAAGGCGTGG GCCGGCCTGG TAAACCGGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGACCC CCAGGACTGA AGGGCATACA GGGAGAGCCC 
GGCCCTCCAG GAGTGGGAGT CCAGGGGCCA CAGGGGGAGC CTGGAGCCCA GGGTCTGCCT 
GGCATTCAGG GGCTTCCGGG CCCTCGGGGA CCACCTGGCC CTGCTGGAGA GAAGGGTGCC 
AAGGGATCTC CAGGAGTGAA AGGAGCCGCT GGGCCTGTGG GACCTCCTGG TGCCAGTGTC 
TCTGGGCCTC CGGGCCCTGA TGGGCAGCAA GGACAGGCTG GACTTCCAGG AGCCAGAGGG 
ATGCCGGGTG AGAAGGGATC ACAGGGAGAG AAGGGCGAGC CGGGGGAGTG TTCCTGCCCC 
TCCCGAGGGG ACCTCATCTT CTCTGGCATG CCGGGTGCTC CGGGACTTTG GATGGGCAGC 
TCCTGGCAGC CGGGCCCGCA GGGTCCTCCG GGTGTTCCTG GACCACCAGG CCCTCCTGGA 
ATGCCTGGGC TGCAGGGGGT GCCCGGGCAC AACGGTTTGC CAGGACAGCC CGGGCTAACT 
GCAGAACTGG GATCCTTGCC CATTGAACAG CACCTCCTGA AGAGCATCTG CGGGGACTGT 
ATCCAGGGCA AGATGGCTCA CGAGGTGGCC CTCATGGAAA GCGGAGAGAA GGGAGACCAG 
GGCATCCCCG GCGTGCCAGG CCTCGACAGT TGCACCCAGT GCTTCATGGA GCGGGAGCGC 
CCGAGAGCCG AGGAGGCCCG GGGGGACAAC CATGAGGGAG ACCCGGGCTG TGCAGGGAGC 
CCCGGGCTGC CGGGACCTCC GGGACTGCCT GGCCAGAGAG GAGAAGAGGG TCCACCTGGT 
ATGAGGGGCT CCCCGGGTCC TCCAGGCCCT ATTGGTCTTC AAGGAGAGCG AGGCCTCAGG 
GGCCTGACGG GAGAGAAGGG GGAGCCGGGT CCTCCAGGGC AACCTGGTTA CCCAGGCGCC 
ATGGGCCCCC CAGGACTGCC TGGCATCAAG GGGGAGCGAG GCTACGCGGG TTCCCCGGGA 
GAGAAGGGAG AATCGGGCCC ACCAGGATCT GAAGGCCTCC CAGGGCCCCC AGGCCCAGCG 
GGTCCCCGAG GAGAGCGAGG GCCCCAAGGA AGCTCGGGTG AGAAAGGAGA CCAGGGATTC 
CAAGGGCAAC CCGGCTTTCC AGGCCCCCCG GGTCCCCCTG GATTCCCAGG CAAAGCTGGA 
GCACCTGGCC CACCCGGCCC CCAAGCGGAT AAGGGCAGTG AAGGGATTCG AGGCCCATCA 
GGTCTGCCTG GTCCCCCTGG ACCACCAGGA TCTCCCGGGA TTCAGGGCCC CGCCGGTCTG 
GATGGTCTGG ATGGGAAGGA TGGCAAACCT GGCTTGAGGG GGGACCCTGG TCCTGCTGGC 
CCTCCCGGAC TCATGGGACC CCCGGGCTTC AAGGGAAAAC CCGGCCATCC TGGCCTCCCA 
GGACAGAAGG GCGACTGTGG AAAGCCAGGC CCCCAGGGCA GCAGTGGCCG GCCCGGAGCA 
GAGGGTGAGC CTGGTGCCAT GGGACCCCAG GGAAGACCCG GCCCCCCTGG CCATGTTGGG 
CCGCCGGGGC CTCCAGGCCA GCCAGGCCCA GCTGGGATCT CTGCAGTGGG CCCAAAAGGA 
GACCGTGGAG CCACTGGAGA AAGGGGCCTT TTAGGTCTTC CAGGCCAACC TGGCCCCCCT 
GGACACCCTG GTCCCCCGGG TGAACCCGGC ATGGATGGCG CAGCTGGCAA AGAGGGACCC 
CCTGGAAAAC AGGGACTCTA TGGACCCCCA GGTCCAAAGG GTGACCCGGG GCCTTCAGGA 
CAGAAGGGCC AGGCAGGAGA GAAGGGAAGA GCTGGCATGC CTGGCGGGCC CGGCAAGAGT 
GGCTCCATGG GACCTGCGGG GCCACCGGGT CCTGCAGGAG AGAGAGGCCA CCCTGGATCT 
CCGGGGCCTG CGGGGAGCCC TGGATTGCCC GGGGTGCCCG GCTCCATGGG AGACATGGTG 
AATTATGATG AAATCAAGAG GTTCATCAGA CAAGAGATCA TTAAAATGTT TGATGAGAGG 
ATGGCTTACT ACACCTCCAG GATGCAGTTT CCCATGGAGA TGGCAGCAGC TCCAGGGCGA 
CCAGGGCCCC CAGGGAAGGA TGGTCCCCCA GGCCGGCCAG GCTCCCCGGG ATCACCGGGA 
CTCCCCGGTC AGATTGGCAG AGAAGGACGG CAGGGCTTGC CCGGGATGAG AGGGTTGCCT 
GGTACCAAAG GTGAAAAGGG AGACATCGGT GTTGGCATCG CAGGAGAAAT TGGTCTTCCT 
GGCCCCCCAG GTCCCCAGGG TCCTCCAGGG TACGGCAAGA TGGGTGCGAC TGGGCCGATG 
GGCCAGCAAG GCATTCCTGG CATCCCCGGT CCCCCAGGCC CCATAGGCCC GCCGGGCAAG 
ACCGGCCACT GTAACCCCTC TGACTGCTTT GGGGCCATGC CGATGGAGCA ACAGTACCCG 
CCCATGAAAA ACATGAAGGG ACCTTTCGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015462430.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317916.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa88038
Clone ID Related Accession (Same CDS sequence)	XM_015462430.1 , XM_015459964.1
Accession Version	XM_015462430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCTTCAAGG AGAGCGAGGC CTCAGGGGCC TGACGGGAGA GAAGGGGGAG 
CCGGGTCCTC CAGGGCAACC TGGTTACCCA GGCGCCATGG GCCCCCCAGG ACTGCCTGGC 
ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC CCGGGAGAGA AGGGAGAATC GGGCCCACCA 
GGATCTGAAG GCCTCCCAGG GCCCCCAGGC CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC 
CAAGGAAGCT CGGGTGAGAA AGGAGACCAG GGATTCCAAG GGCAACCCGG CTTTCCAGGC 
CCCCCGGGTC CCCCTGGATT CCCAGGCAAA GCTGGAGCAC CTGGCCCACC CGGCCCCCAA 
GCGGATAAGG GCAGTGAAGG GATTCGAGGC CCATCAGGTC TGCCTGGTCC CCCTGGACCA 
CCAGGATCTC CCGGGATTCA GGGCCCCGCC GGTCTGGATG GTCTGGATGG GAAGGATGGC 
AAACCTGGCT TGAGGGGGGA CCCTGGTCCT GCTGGCCCTC CCGGACTCAT GGGACCCCCG 
GGCTTCAAGG GAAAACCCGG CCATCCTGGC CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG 
CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC GGAGCAGAGG GTGAGCCTGG TGCCATGGGA 
CCCCAGGGAA GACCCGGCCC CCCTGGCCAT GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA 
GGCCCAGCTG GGATCTCTGC AGTGGGCCCA AAAGGAGACC GTGGAGCCAC TGGAGAAAGG 
GGCCTTTTAG GTCTTCCAGG CCAACCTGGC CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA 
CCCGGCATGG ATGGCGCAGC TGGCAAAGAG GGACCCCCTG GAAAACAGGG ACTCTATGGA 
CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT TCAGGACAGA AGGGCCAGGC AGGAGAGAAG 
GGAAGAGCTG GCATGCCTGG CGGGCCCGGC AAGAGTGGCT CCATGGGACC TGCGGGGCCA 
CCGGGTCCTG CAGGAGAGAG AGGCCACCCT GGATCTCCGG GGCCTGCGGG GAGCCCTGGA 
TTGCCCGGGG TGCCCGGCTC CATGGGAGAC ATGGTGAATT ATGATGAAAT CAAGAGGTTC 
ATCAGACAAG AGATCATTAA AATGTTTGAT GAGAGGATGG CTTACTACAC CTCCAGGATG 
CAGTTTCCCA TGGAGATGGC AGCAGCTCCA GGGCGACCAG GGCCCCCAGG GAAGGATGGT 
CCCCCAGGCC GGCCAGGCTC CCCGGGATCA CCGGGACTCC CCGGTCAGAT TGGCAGAGAA 
GGACGGCAGG GCTTGCCCGG GATGAGAGGG TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC 
ATCGGTGTTG GCATCGCAGG AGAAATTGGT CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT 
CCAGGGTACG GCAAGATGGG TGCGACTGGG CCGATGGGCC AGCAAGGCAT TCCTGGCATC 
CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG GGCAAGACCG GCCACTGTAA CCCCTCTGAC 
TGCTTTGGGG CCATGCCGAT GGAGCAACAG TACCCGCCCA TGAAAAACAT GAAGGGACCT 
TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317916.1
CDS	207..5015
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015462430.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462430.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCTTCAAGG AGAGCGAGGC CTCAGGGGCC TGACGGGAGA GAAGGGGGAG 
CCGGGTCCTC CAGGGCAACC TGGTTACCCA GGCGCCATGG GCCCCCCAGG ACTGCCTGGC 
ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC CCGGGAGAGA AGGGAGAATC GGGCCCACCA 
GGATCTGAAG GCCTCCCAGG GCCCCCAGGC CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC 
CAAGGAAGCT CGGGTGAGAA AGGAGACCAG GGATTCCAAG GGCAACCCGG CTTTCCAGGC 
CCCCCGGGTC CCCCTGGATT CCCAGGCAAA GCTGGAGCAC CTGGCCCACC CGGCCCCCAA 
GCGGATAAGG GCAGTGAAGG GATTCGAGGC CCATCAGGTC TGCCTGGTCC CCCTGGACCA 
CCAGGATCTC CCGGGATTCA GGGCCCCGCC GGTCTGGATG GTCTGGATGG GAAGGATGGC 
AAACCTGGCT TGAGGGGGGA CCCTGGTCCT GCTGGCCCTC CCGGACTCAT GGGACCCCCG 
GGCTTCAAGG GAAAACCCGG CCATCCTGGC CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG 
CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC GGAGCAGAGG GTGAGCCTGG TGCCATGGGA 
CCCCAGGGAA GACCCGGCCC CCCTGGCCAT GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA 
GGCCCAGCTG GGATCTCTGC AGTGGGCCCA AAAGGAGACC GTGGAGCCAC TGGAGAAAGG 
GGCCTTTTAG GTCTTCCAGG CCAACCTGGC CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA 
CCCGGCATGG ATGGCGCAGC TGGCAAAGAG GGACCCCCTG GAAAACAGGG ACTCTATGGA 
CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT TCAGGACAGA AGGGCCAGGC AGGAGAGAAG 
GGAAGAGCTG GCATGCCTGG CGGGCCCGGC AAGAGTGGCT CCATGGGACC TGCGGGGCCA 
CCGGGTCCTG CAGGAGAGAG AGGCCACCCT GGATCTCCGG GGCCTGCGGG GAGCCCTGGA 
TTGCCCGGGG TGCCCGGCTC CATGGGAGAC ATGGTGAATT ATGATGAAAT CAAGAGGTTC 
ATCAGACAAG AGATCATTAA AATGTTTGAT GAGAGGATGG CTTACTACAC CTCCAGGATG 
CAGTTTCCCA TGGAGATGGC AGCAGCTCCA GGGCGACCAG GGCCCCCAGG GAAGGATGGT 
CCCCCAGGCC GGCCAGGCTC CCCGGGATCA CCGGGACTCC CCGGTCAGAT TGGCAGAGAA 
GGACGGCAGG GCTTGCCCGG GATGAGAGGG TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC 
ATCGGTGTTG GCATCGCAGG AGAAATTGGT CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT 
CCAGGGTACG GCAAGATGGG TGCGACTGGG CCGATGGGCC AGCAAGGCAT TCCTGGCATC 
CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG GGCAAGACCG GCCACTGTAA CCCCTCTGAC 
TGCTTTGGGG CCATGCCGAT GGAGCAACAG TACCCGCCCA TGAAAAACAT GAAGGGACCT 
TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL16A1 ( XM_015459964.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315450.1 Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X6, mRNA.

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CloneID	OBa88038
Clone ID Related Accession (Same CDS sequence)	XM_015462430.1 , XM_015459964.1
Accession Version	XM_015459964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(XVI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCTTCAAGG AGAGCGAGGC CTCAGGGGCC TGACGGGAGA GAAGGGGGAG 
CCGGGTCCTC CAGGGCAACC TGGTTACCCA GGCGCCATGG GCCCCCCAGG ACTGCCTGGC 
ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC CCGGGAGAGA AGGGAGAATC GGGCCCACCA 
GGATCTGAAG GCCTCCCAGG GCCCCCAGGC CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC 
CAAGGAAGCT CGGGTGAGAA AGGAGACCAG GGATTCCAAG GGCAACCCGG CTTTCCAGGC 
CCCCCGGGTC CCCCTGGATT CCCAGGCAAA GCTGGAGCAC CTGGCCCACC CGGCCCCCAA 
GCGGATAAGG GCAGTGAAGG GATTCGAGGC CCATCAGGTC TGCCTGGTCC CCCTGGACCA 
CCAGGATCTC CCGGGATTCA GGGCCCCGCC GGTCTGGATG GTCTGGATGG GAAGGATGGC 
AAACCTGGCT TGAGGGGGGA CCCTGGTCCT GCTGGCCCTC CCGGACTCAT GGGACCCCCG 
GGCTTCAAGG GAAAACCCGG CCATCCTGGC CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG 
CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC GGAGCAGAGG GTGAGCCTGG TGCCATGGGA 
CCCCAGGGAA GACCCGGCCC CCCTGGCCAT GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA 
GGCCCAGCTG GGATCTCTGC AGTGGGCCCA AAAGGAGACC GTGGAGCCAC TGGAGAAAGG 
GGCCTTTTAG GTCTTCCAGG CCAACCTGGC CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA 
CCCGGCATGG ATGGCGCAGC TGGCAAAGAG GGACCCCCTG GAAAACAGGG ACTCTATGGA 
CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT TCAGGACAGA AGGGCCAGGC AGGAGAGAAG 
GGAAGAGCTG GCATGCCTGG CGGGCCCGGC AAGAGTGGCT CCATGGGACC TGCGGGGCCA 
CCGGGTCCTG CAGGAGAGAG AGGCCACCCT GGATCTCCGG GGCCTGCGGG GAGCCCTGGA 
TTGCCCGGGG TGCCCGGCTC CATGGGAGAC ATGGTGAATT ATGATGAAAT CAAGAGGTTC 
ATCAGACAAG AGATCATTAA AATGTTTGAT GAGAGGATGG CTTACTACAC CTCCAGGATG 
CAGTTTCCCA TGGAGATGGC AGCAGCTCCA GGGCGACCAG GGCCCCCAGG GAAGGATGGT 
CCCCCAGGCC GGCCAGGCTC CCCGGGATCA CCGGGACTCC CCGGTCAGAT TGGCAGAGAA 
GGACGGCAGG GCTTGCCCGG GATGAGAGGG TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC 
ATCGGTGTTG GCATCGCAGG AGAAATTGGT CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT 
CCAGGGTACG GCAAGATGGG TGCGACTGGG CCGATGGGCC AGCAAGGCAT TCCTGGCATC 
CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG GGCAAGACCG GCCACTGTAA CCCCTCTGAC 
TGCTTTGGGG CCATGCCGAT GGAGCAACAG TACCCGCCCA TGAAAAACAT GAAGGGACCT 
TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315450.1
CDS	106..4914
Translation	MWASWAPGLWLLSLYVTYSHGADAGEQCPPSQQEGLKLEHRSDLSTNVTGFNLIHRLSLMKTSAVKKIRNPKGPFILRLGAVSVTQPTRRVFPRGLPSEFALVLTLLMKKHTHRNTWYLFQVTDGDGYPQISLEVNSQEQSLELRTRGQDGDFVSCIFSVPQLFDLRWHKLVLSVAERVASVHVDCMSASSQPLGPRRSIEPVGHVFLGLDAEQGKPVSFDLQQAHIYCDPEFVLEEGCCEILPGGPFPNTGQCPPETSKARRDTQNNELIEINPQTEGKVYTRCFCLEEPQNSKVDAQLSGRISQKPERGAKAHQETAADECPPCVHGAQESNVTIGPSGPQGGKGERGLPGPSGPKGEKGARGNDCVRISPDAPLQCAEGLKGEKGESGALGPSGLPGSTGQKGQKGEKGDGGVKGSPGKPGRDGRPGEVCVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKGNSGVPGKEGPGGKPGKPGVPGLKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPARESLGAVGQKGDRGDPGIQGLKGEKGESCSSCSFAVAAQHLQPSAGAKGDVGPPGLGLPGLPGKAGAPGPTGLKGEKGNFGEVGPAGNPGPPGPAGPTGIKGEKGEPCEPCRALTEHQDEDSHVVALPGPRGEKGEPGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGITGRPGLSGEPGVRGPVGPKGEKGDGCTACPSTQGALTDRAGPPGKPGPKGDPGPEGVGRPGKPGQPGLPGVQGPPGLKGIQGEPGPPGVGVQGPQGEPGAQGLPGIQGLPGPRGPPGPAGEKGAKGSPGVKGAAGPVGPPGASVSGPPGPDGQQGQAGLPGARGMPGEKGSQGEKGEPGECSCPSRGDLIFSGMPGAPGLWMGSSWQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCIQGKMAHEVALMESGEKGDQGIPGVPGLDSCTQCFMERERPRAEEARGDNHEGDPGCAGSPGLPGPPGLPGQRGEEGLQGERGLRGLTGEKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGSPGEKGESGPPGSEGLPGPPGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQADKGSEGIRGPSGLPGPPGPPGSPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKPGHPGLPGQKGDCGKPGPQGSSGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGPKGDRGATGERGLLGLPGQPGPPGHPGPPGEPGMDGAAGKEGPPGKQGLYGPPGPKGDPGPSGQKGQAGEKGRAGMPGGPGKSGSMGPAGPPGPAGERGHPGSPGPAGSPGLPGVPGSMGDMVNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGPPGRPGSPGSPGLPGQIGREGRQGLPGMRGLPGTKGEKGDIGVGIAGEIGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPIGPPGKTGHCNPSDCFGAMPMEQQYPPMKNMKGPFG

Target ORF information:

RefSeq Version	XM_015459964.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type XVI alpha 1 (COL16A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459964.1

ORF Insert Sequence:

ATGTGGGCAT CTTGGGCTCC TGGCTTGTGG CTGCTCAGTC TGTATGTTAC CTACAGCCAT 
GGAGCAGATG CAGGGGAGCA GTGCCCACCT TCTCAGCAAG AAGGACTCAA GTTGGAACAC 
AGAAGTGACT TATCGACCAA CGTGACTGGC TTCAACCTCA TCCACCGACT CAGCCTCATG 
AAGACATCTG CTGTCAAGAA GATCCGAAAT CCCAAGGGAC CATTCATCTT GCGACTGGGA 
GCAGTCTCAG TCACCCAGCC CACTCGACGC GTGTTCCCTC GGGGCCTTCC CAGTGAGTTT 
GCCCTGGTGC TGACACTATT AATGAAGAAA CACACCCACC GGAACACGTG GTATTTGTTT 
CAAGTGACCG ATGGAGACGG GTACCCGCAG ATTTCTCTGG AAGTCAACAG TCAGGAGCAG 
AGCCTGGAGT TAAGGACCCG GGGTCAAGAT GGTGACTTTG TGTCCTGCAT CTTCTCAGTG 
CCCCAGCTCT TCGACCTGCG TTGGCATAAG CTGGTGCTGA GCGTGGCCGA ACGAGTGGCC 
TCCGTGCACG TGGACTGCAT GTCAGCCTCC TCCCAGCCTC TAGGGCCCCG GCGATCCATA 
GAGCCTGTGG GCCATGTCTT TCTGGGCTTA GATGCTGAGC AGGGCAAACC TGTCTCGTTT 
GACCTTCAGC AGGCACACAT CTACTGTGAC CCAGAGTTCG TGCTGGAGGA GGGCTGCTGT 
GAGATATTAC CAGGAGGGCC CTTCCCCAAC ACTGGACAGT GTCCCCCAGA GACCTCCAAG 
GCCCGCCGGG ACACCCAGAA CAATGAGCTC ATCGAGATCA ATCCACAGAC TGAGGGCAAG 
GTCTACACCC GCTGCTTCTG CCTGGAGGAG CCCCAGAACA GCAAGGTGGA TGCCCAGCTG 
TCGGGAAGAA TCAGCCAGAA GCCAGAAAGA GGAGCAAAGG CCCACCAGGA GACAGCAGCC 
GATGAGTGCC CACCCTGTGT CCATGGTGCC CAGGAGAGCA ACGTCACAAT TGGTCCTTCT 
GGCCCCCAGG GAGGGAAAGG TGAGCGGGGC CTGCCTGGTC CATCAGGACC CAAGGGAGAG 
AAGGGAGCCC GGGGCAATGA CTGTGTTAGA ATCTCCCCGG ATGCCCCGCT TCAGTGTGCA 
GAAGGCCTGA AGGGAGAGAA GGGAGAGTCG GGAGCCTTGG GACCCTCAGG ACTCCCCGGC 
TCCACAGGCC AGAAGGGCCA GAAAGGCGAG AAGGGAGACG GAGGCGTGAA GGGCTCGCCG 
GGAAAGCCAG GCCGAGATGG CCGGCCAGGA GAAGTCTGTG TCATAGGGCC CAAAGGGCAG 
AAGGGAGACC CTGGCTTTGT TGGGCCTGAG GGGCTGGCAG GAGAGCCCGG GCCCCCAGGC 
CTCCCTGGGC CCCCTGGCAT AGGACTTCCG GGGACCCCGG GGGACCCAGG TGGCCCTCCA 
GGCCCCAAGG GAGACAAGGG CAACTCCGGG GTGCCTGGAA AGGAAGGTCC TGGCGGGAAA 
CCTGGCAAAC CAGGGGTGCC CGGGCTGAAG GGAGAGAAGG GTGACCCCTG TGAAGTGTGC 
CCAACACTAC CTGAAGGGTT CCAGAACTTT GTGGGGCTCC CTGGAAAGCC AGGACCCAAG 
GGAGAACCAG GTGATCCTGC ACCAGCCAGG GAGAGTCTGG GAGCTGTTGG ACAAAAAGGT 
GATCGGGGAG ACCCTGGCAT CCAAGGCCTC AAAGGAGAGA AGGGGGAGTC CTGCTCGTCC 
TGCAGCTTTG CCGTAGCGGC CCAGCATCTC CAGCCTTCTG CAGGGGCCAA AGGCGATGTG 
GGCCCTCCTG GCCTGGGTTT GCCAGGTCTT CCGGGGAAAG CTGGGGCCCC AGGGCCAACA 
GGGCTGAAAG GAGAAAAGGG TAATTTCGGG GAGGTAGGGC CAGCCGGCAA TCCGGGGCCG 
CCAGGGCCTG CGGGACCCAC AGGCATCAAA GGGGAGAAGG GGGAGCCTTG TGAGCCATGC 
AGAGCCCTGA CCGAGCATCA GGATGAAGAC AGCCACGTGG TGGCCTTGCC GGGGCCCCGC 
GGAGAGAAGG GGGAGCCCGG GCCTCCAGGT TTCGGTCTGC CAGGAAAACA GGGCAAGGCT 
GGAGAGCGTG GACTGAAGGG GCAAAAGGGT GATGCTGGGA ATCCTGGAGA CCCTGGAACA 
CCAGGCATCA CGGGGCGGCC AGGACTGTCG GGAGAGCCAG GGGTTCGGGG CCCTGTGGGG 
CCAAAAGGAG AAAAGGGTGA TGGCTGCACC GCCTGCCCCA GCACGCAGGG GGCGCTGACA 
GACAGAGCGG GGCCGCCTGG GAAGCCGGGC CCGAAAGGAG ATCCTGGGCC AGAAGGCGTG 
GGCCGGCCTG GTAAACCGGG CCAGCCTGGT CTACCAGGAG TTCAAGGACC CCCAGGACTG 
AAGGGCATAC AGGGAGAGCC CGGCCCTCCA GGAGTGGGAG TCCAGGGGCC ACAGGGGGAG 
CCTGGAGCCC AGGGTCTGCC TGGCATTCAG GGGCTTCCGG GCCCTCGGGG ACCACCTGGC 
CCTGCTGGAG AGAAGGGTGC CAAGGGATCT CCAGGAGTGA AAGGAGCCGC TGGGCCTGTG 
GGACCTCCTG GTGCCAGTGT CTCTGGGCCT CCGGGCCCTG ATGGGCAGCA AGGACAGGCT 
GGACTTCCAG GAGCCAGAGG GATGCCGGGT GAGAAGGGAT CACAGGGAGA GAAGGGCGAG 
CCGGGGGAGT GTTCCTGCCC CTCCCGAGGG GACCTCATCT TCTCTGGCAT GCCGGGTGCT 
CCGGGACTTT GGATGGGCAG CTCCTGGCAG CCGGGCCCGC AGGGTCCTCC GGGTGTTCCT 
GGACCACCAG GCCCTCCTGG AATGCCTGGG CTGCAGGGGG TGCCCGGGCA CAACGGTTTG 
CCAGGACAGC CCGGGCTAAC TGCAGAACTG GGATCCTTGC CCATTGAACA GCACCTCCTG 
AAGAGCATCT GCGGGGACTG TATCCAGGGC AAGATGGCTC ACGAGGTGGC CCTCATGGAA 
AGCGGAGAGA AGGGAGACCA GGGCATCCCC GGCGTGCCAG GCCTCGACAG TTGCACCCAG 
TGCTTCATGG AGCGGGAGCG CCCGAGAGCC GAGGAGGCCC GGGGGGACAA CCATGAGGGA 
GACCCGGGCT GTGCAGGGAG CCCCGGGCTG CCGGGACCTC CGGGACTGCC TGGCCAGAGA 
GGAGAAGAGG GTCTTCAAGG AGAGCGAGGC CTCAGGGGCC TGACGGGAGA GAAGGGGGAG 
CCGGGTCCTC CAGGGCAACC TGGTTACCCA GGCGCCATGG GCCCCCCAGG ACTGCCTGGC 
ATCAAGGGGG AGCGAGGCTA CGCGGGTTCC CCGGGAGAGA AGGGAGAATC GGGCCCACCA 
GGATCTGAAG GCCTCCCAGG GCCCCCAGGC CCAGCGGGTC CCCGAGGAGA GCGAGGGCCC 
CAAGGAAGCT CGGGTGAGAA AGGAGACCAG GGATTCCAAG GGCAACCCGG CTTTCCAGGC 
CCCCCGGGTC CCCCTGGATT CCCAGGCAAA GCTGGAGCAC CTGGCCCACC CGGCCCCCAA 
GCGGATAAGG GCAGTGAAGG GATTCGAGGC CCATCAGGTC TGCCTGGTCC CCCTGGACCA 
CCAGGATCTC CCGGGATTCA GGGCCCCGCC GGTCTGGATG GTCTGGATGG GAAGGATGGC 
AAACCTGGCT TGAGGGGGGA CCCTGGTCCT GCTGGCCCTC CCGGACTCAT GGGACCCCCG 
GGCTTCAAGG GAAAACCCGG CCATCCTGGC CTCCCAGGAC AGAAGGGCGA CTGTGGAAAG 
CCAGGCCCCC AGGGCAGCAG TGGCCGGCCC GGAGCAGAGG GTGAGCCTGG TGCCATGGGA 
CCCCAGGGAA GACCCGGCCC CCCTGGCCAT GTTGGGCCGC CGGGGCCTCC AGGCCAGCCA 
GGCCCAGCTG GGATCTCTGC AGTGGGCCCA AAAGGAGACC GTGGAGCCAC TGGAGAAAGG 
GGCCTTTTAG GTCTTCCAGG CCAACCTGGC CCCCCTGGAC ACCCTGGTCC CCCGGGTGAA 
CCCGGCATGG ATGGCGCAGC TGGCAAAGAG GGACCCCCTG GAAAACAGGG ACTCTATGGA 
CCCCCAGGTC CAAAGGGTGA CCCGGGGCCT TCAGGACAGA AGGGCCAGGC AGGAGAGAAG 
GGAAGAGCTG GCATGCCTGG CGGGCCCGGC AAGAGTGGCT CCATGGGACC TGCGGGGCCA 
CCGGGTCCTG CAGGAGAGAG AGGCCACCCT GGATCTCCGG GGCCTGCGGG GAGCCCTGGA 
TTGCCCGGGG TGCCCGGCTC CATGGGAGAC ATGGTGAATT ATGATGAAAT CAAGAGGTTC 
ATCAGACAAG AGATCATTAA AATGTTTGAT GAGAGGATGG CTTACTACAC CTCCAGGATG 
CAGTTTCCCA TGGAGATGGC AGCAGCTCCA GGGCGACCAG GGCCCCCAGG GAAGGATGGT 
CCCCCAGGCC GGCCAGGCTC CCCGGGATCA CCGGGACTCC CCGGTCAGAT TGGCAGAGAA 
GGACGGCAGG GCTTGCCCGG GATGAGAGGG TTGCCTGGTA CCAAAGGTGA AAAGGGAGAC 
ATCGGTGTTG GCATCGCAGG AGAAATTGGT CTTCCTGGCC CCCCAGGTCC CCAGGGTCCT 
CCAGGGTACG GCAAGATGGG TGCGACTGGG CCGATGGGCC AGCAAGGCAT TCCTGGCATC 
CCCGGTCCCC CAGGCCCCAT AGGCCCGCCG GGCAAGACCG GCCACTGTAA CCCCTCTGAC 
TGCTTTGGGG CCATGCCGAT GGAGCAACAG TACCCGCCCA TGAAAAACAT GAAGGGACCT 
TTCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL16A1
Entrez Gene ID	507010
Full Name	collagen type XVI alpha 1 chain
General protein information	Preferred Names collagen type XVI alpha 1 chain Names collagen alpha-1(XVI) chain collagen, type XVI, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	COL16A1	NP_001847.3
Mus musculus (house mouse)	Col16a1	NP_082542.3
Rattus norvegicus (Norway rat)	Col16a1	NP_001015033.2
Xenopus tropicalis (tropical clawed frog)	LOC100497018	XP_004919282.1
Pan troglodytes (chimpanzee)	COL16A1	XP_513270.4
Gallus gallus (chicken)	COL16A1	XP_003642629.2
	COL16A1	XP_001100182.1
Canis lupus familiaris (dog)	COL16A1	XP_544444.3
Bos taurus (cattle)	COL16A1	XP_005203115.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL16A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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