SEMA5A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA5A
Entrez Gene ID	506636
Full Name	semaphorin 5A
General protein information	Preferred Names semaphorin 5A Names semaphorin-5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002696441.5	XP_002696487.1	semaphorin-5A
XM_015468386.1	XP_015323872.1	semaphorin-5A
XM_002696441.6	XP_002696487.2	semaphorin-5A
XM_010816848.3	XP_010815150.2	semaphorin-5A
XM_015468387.1	XP_015323873.1	semaphorin-5A
XM_010816848.2	XP_010815150.1	semaphorin-5A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SEMA5A	XP_005639731.1
Rattus norvegicus (Norway rat)	Sema5a	NP_001101129.1
Bos taurus (cattle)	SEMA5A	XP_005221710.1
Gallus gallus (chicken)	SEMA5A	XP_419016.3
Danio rerio (zebrafish)	sema5a	NP_001258599.1
Pan troglodytes (chimpanzee)	SEMA5A	XP_517627.2
Mus musculus (house mouse)	Sema5a	NP_033180.2
Homo sapiens (human)	SEMA5A	NP_003957.2
	SEMA5A	XP_002804352.1

Gene Ontology

The following SEMA5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228172	XM_002696441.6 Latest version!	Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa228172	XM_010816848.3 Latest version!	Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa30717	XM_015468386.1 Latest version!	Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30717	XM_015468387.1 Latest version!	Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30717	XM_002696441.5 Latest version!	Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30717	XM_010816848.2 Latest version!	Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA5A ( XM_002696441.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696487.2 Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228172
Clone ID Related Accession (Same CDS sequence)	XM_002696441.6 , XM_010816848.3
Accession Version	XM_002696441.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696441.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACATGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACGCCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696487.2
CDS	671..3892
Translation	MKGPCVIAWLFSSLGWWRLAQLETDASLCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAAEWECDEATKKACYSKGKSQEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRTLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYHELQGTFFLPELDLIYGIFTTNVNSIAASAVCIFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTVDQGVYMNLTERNLQDAQKFILMHEVVQPVSPVPAFMEDSSRFSHVAVDVVQARDALVHVIYLATDYGTIKKVRAPLTQTSGGCVLEEMELFPERRPEPVRSLRILHSQSVLFAGLREHVAKIPLKRCQFYRSRSACIGAQDPYCGWDLVMKKCTSLEESLSMTQWEQSIATCPVRNLTVDGHFGAWSPWTPCTHMDGSAVGSCLCRTRSCDSPAPQCGGWQCQGPRMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEYQECNILPCPVDGMWSCWSSWSTCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSEWSECDVAGVQVRVRQCVLLFPVGSQCSGNTTESRPCIFDSNFIPEVSVARSSSIEEKRCGEFNLFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVAPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_002696441.6
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696441.6

ORF Insert Sequence:

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACATGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACGCCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5A ( XM_010816848.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815150.2 Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa228172
Clone ID Related Accession (Same CDS sequence)	XM_002696441.6 , XM_010816848.3
Accession Version	XM_010816848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACATGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACGCCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815150.2
CDS	357..3578
Translation	MKGPCVIAWLFSSLGWWRLAQLETDASLCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAAEWECDEATKKACYSKGKSQEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRTLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYHELQGTFFLPELDLIYGIFTTNVNSIAASAVCIFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTVDQGVYMNLTERNLQDAQKFILMHEVVQPVSPVPAFMEDSSRFSHVAVDVVQARDALVHVIYLATDYGTIKKVRAPLTQTSGGCVLEEMELFPERRPEPVRSLRILHSQSVLFAGLREHVAKIPLKRCQFYRSRSACIGAQDPYCGWDLVMKKCTSLEESLSMTQWEQSIATCPVRNLTVDGHFGAWSPWTPCTHMDGSAVGSCLCRTRSCDSPAPQCGGWQCQGPRMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEYQECNILPCPVDGMWSCWSSWSTCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSEWSECDVAGVQVRVRQCVLLFPVGSQCSGNTTESRPCIFDSNFIPEVSVARSSSIEEKRCGEFNLFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVAPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_010816848.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816848.3

ORF Insert Sequence:

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACATGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACGCCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5A ( XM_002696441.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696487.1 Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30717
Clone ID Related Accession (Same CDS sequence)	XM_002696441.5 , XM_015468386.1 , XM_015468387.1 , XM_010816848.2
Accession Version	XM_002696441.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104521.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696441.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696487.1
CDS	679..3900
Translation	MKGPCVIAWLFSSLGWWRLAQLETDASLCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAAEWECDEATKKACYSKGKSQEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRTLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYHELQGTFFLPELDLIYGIFTTNVNSIAASAVCIFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTVDQGVYVNLTERNLQDAQKFILMHEVVQPVSPVPAFMEDSSRFSHVAVDVVQARDALVHVIYLATDYGTIKKVRAPLTQTSGGCVLEEMELFPERRPEPVRSLRILHSQSVLFAGLREHVAKIPLKRCQFYRSRSACIGAQDPYCGWDLVMKKCTSLEESLSMTQWEQSIATCPVRNLTVDGHFGAWSPWTPCTHMDGSAVGSCLCRTRSCDSPAPQCGGWQCQGPRMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEYQECNILPCPVDGMWSCWSSWSTCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSEWSECDVAGVQVRVRQCVLLFPVGSQCSGNTTESRPCIFDSNFIPEVSVARSSSIEEKRCGEFNLFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVAPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_002696441.5
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696441.5

ORF Insert Sequence:

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5A ( XM_015468386.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323872.1 Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30717
Clone ID Related Accession (Same CDS sequence)	XM_002696441.5 , XM_015468386.1 , XM_015468387.1 , XM_010816848.2
Accession Version	XM_015468386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323872.1
CDS	679..3900
Translation	MKGPCVIAWLFSSLGWWRLAQLETDASLCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAAEWECDEATKKACYSKGKSQEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRTLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYHELQGTFFLPELDLIYGIFTTNVNSIAASAVCIFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTVDQGVYVNLTERNLQDAQKFILMHEVVQPVSPVPAFMEDSSRFSHVAVDVVQARDALVHVIYLATDYGTIKKVRAPLTQTSGGCVLEEMELFPERRPEPVRSLRILHSQSVLFAGLREHVAKIPLKRCQFYRSRSACIGAQDPYCGWDLVMKKCTSLEESLSMTQWEQSIATCPVRNLTVDGHFGAWSPWTPCTHMDGSAVGSCLCRTRSCDSPAPQCGGWQCQGPRMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEYQECNILPCPVDGMWSCWSSWSTCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSEWSECDVAGVQVRVRQCVLLFPVGSQCSGNTTESRPCIFDSNFIPEVSVARSSSIEEKRCGEFNLFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVAPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_015468386.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468386.1

ORF Insert Sequence:

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5A ( XM_015468387.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323873.1 Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

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CloneID	OBa30717
Clone ID Related Accession (Same CDS sequence)	XM_002696441.5 , XM_015468386.1 , XM_015468387.1 , XM_010816848.2
Accession Version	XM_015468387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323873.1
CDS	754..3975
Translation	MKGPCVIAWLFSSLGWWRLAQLETDASLCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAAEWECDEATKKACYSKGKSQEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRTLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYHELQGTFFLPELDLIYGIFTTNVNSIAASAVCIFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTVDQGVYVNLTERNLQDAQKFILMHEVVQPVSPVPAFMEDSSRFSHVAVDVVQARDALVHVIYLATDYGTIKKVRAPLTQTSGGCVLEEMELFPERRPEPVRSLRILHSQSVLFAGLREHVAKIPLKRCQFYRSRSACIGAQDPYCGWDLVMKKCTSLEESLSMTQWEQSIATCPVRNLTVDGHFGAWSPWTPCTHMDGSAVGSCLCRTRSCDSPAPQCGGWQCQGPRMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEYQECNILPCPVDGMWSCWSSWSTCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSEWSECDVAGVQVRVRQCVLLFPVGSQCSGNTTESRPCIFDSNFIPEVSVARSSSIEEKRCGEFNLFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVAPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_015468387.1
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468387.1

ORF Insert Sequence:

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5A ( XM_010816848.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815150.1 Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

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CloneID	OBa30717
Clone ID Related Accession (Same CDS sequence)	XM_002696441.5 , XM_015468386.1 , XM_015468387.1 , XM_010816848.2
Accession Version	XM_010816848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104521.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815150.1
CDS	754..3975
Translation	MKGPCVIAWLFSSLGWWRLAQLETDASLCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAAEWECDEATKKACYSKGKSQEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRTLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYHELQGTFFLPELDLIYGIFTTNVNSIAASAVCIFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTVDQGVYVNLTERNLQDAQKFILMHEVVQPVSPVPAFMEDSSRFSHVAVDVVQARDALVHVIYLATDYGTIKKVRAPLTQTSGGCVLEEMELFPERRPEPVRSLRILHSQSVLFAGLREHVAKIPLKRCQFYRSRSACIGAQDPYCGWDLVMKKCTSLEESLSMTQWEQSIATCPVRNLTVDGHFGAWSPWTPCTHMDGSAVGSCLCRTRSCDSPAPQCGGWQCQGPRMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEYQECNILPCPVDGMWSCWSSWSTCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSEWSECDVAGVQVRVRQCVLLFPVGSQCSGNTTESRPCIFDSNFIPEVSVARSSSIEEKRCGEFNLFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVAPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_010816848.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816848.2

ORF Insert Sequence:

ATGAAGGGAC CCTGCGTCAT CGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
CAGCTAGAGA CAGATGCATC TCTGTGCCAG AGAGCAGAGC ACCCAGTCAT TTCCTATAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGATC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA 
CAGCTGGAGG ATTTGTCTCT GATCCAGGCT GCGGAATGGG AGTGTGATGA AGCAACCAAG 
AAGGCCTGTT ACAGCAAAGG CAAGTCCCAG GAGGAATGTC AGAACTACAT TCGGGTACTG 
TTGGTGGGTG GTGACCGGTT ATTCACGTGT GGGACCAATG CTTTCACGCC TGTCTGCACG 
AACCGCACGT TGAGTAACCT GACGGAGATC CATGACCAGA TCAGCGGCAT GGCACGCTGC 
CCCTACAGCC CCCAGCACAA CTCCACAGCC CTGCTCACAG CCGGCGGGGA GCTTTATGCT 
GCCACGGCCA TGGATTTCCC GGGACGCGAT CCCGCCATTT ACCGAAGCCT GGGCATCTTG 
CCCCCGCTCC GCACCGCACA GTACAACTCC AAGTGGCTCA ACGAACCAAA CTTCGTGTCC 
TCTTACGACA TCGGAAATTT CACCTACTTC TTCTTCCGAG AAAATGCCGT GGAGCACGAC 
TGTGGGAAGA CCGTGTTCTC CCGAGCTGCC CGCGTGTGTA AGAACGACAT CGGGGGCCGC 
TTCCTGCTGG AGGACACGTG GACCACGTTC ATGAAGGCTC GCCTCAACTG CTCCCGCCCG 
GGTGAGGTGC CTTTCTACTA CCATGAACTC CAGGGCACCT TCTTCCTGCC CGAGCTGGAT 
CTGATCTACG GCATCTTCAC CACCAATGTG AACAGCATCG CTGCCTCGGC CGTGTGCATC 
TTCAACCTCA GCGCCATCTC TCAGGCCTTT AACGGGCCCT TCAAGTACCA GGAGAACTCT 
CGGTCCGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGTGG CACCGTGGAC 
CAGGGTGTGT ACGTGAACCT CACGGAGAGG AACCTGCAGG ACGCGCAGAA GTTCATCCTG 
ATGCACGAAG TGGTGCAGCC GGTGAGCCCC GTGCCCGCCT TCATGGAGGA CAGCAGCCGC 
TTCTCCCACG TGGCCGTGGA TGTGGTGCAG GCCCGGGACG CCCTCGTCCA CGTCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAG GTGCGGGCGC CCCTGACCCA GACCTCTGGC 
GGCTGTGTGC TGGAAGAGAT GGAGCTCTTC CCTGAGAGGC GACCCGAGCC CGTCAGGAGC 
CTGCGGATCC TGCACAGCCA GAGCGTCCTG TTCGCGGGCC TGCGAGAGCA CGTGGCCAAG 
ATCCCGCTGA AGAGGTGCCA GTTCTACCGC TCACGCAGTG CCTGCATTGG GGCCCAGGAC 
CCGTACTGTG GCTGGGACCT GGTGATGAAG AAATGCACGA GCCTGGAGGA GAGCCTGAGC 
ATGACGCAGT GGGAGCAGAG CATCGCGACC TGTCCTGTGA GGAATCTCAC TGTGGATGGG 
CACTTTGGCG CGTGGTCTCC GTGGACACCC TGCACCCACA TGGACGGCAG CGCCGTGGGA 
TCCTGCCTCT GTCGCACCCG CTCCTGCGAC AGCCCGGCCC CCCAGTGTGG TGGCTGGCAG 
TGCCAGGGCC CCCGCATGGA GATCGCGAAC TGCTCCAGGA ACGGAGGCTG GACCCCCTGG 
ACCTCGTGGT CTCCCTGCAG CACCACGTGT GGGATCGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACCCCGCG GCACGGGGGC CGGGTGTGCG TCGGGCAGAA CCGCGAGGAG 
AGATACTGCA ATGAGCACCT GCTGTGCCCG CCACACGTCT TCTGGACAGG ATGGGGACCC 
TGGGAACGGT GCACAGCCCA GTGTGGGGGT GGCATTCAAG CTCGCCGCAG AACCTGCGAG 
AACGGGCCTG ACTGTGCAGG CTGTAACGTG GAGTACCAGC CTTGTAACAC CAACCCATGC 
CCTGAGCTGA AAAAGACCAC GCCCTGGACT CCCTGGACGC CCGTCAACAT CTCTGACAAT 
GGGGGTCACT ACGAGCAGCG ATTTCGGTAC ACGTGCAAAG CCCGTCTGCC TGATCCAAAC 
TTGCTGGAAG TGGGAAGACA GAGAATTGAG ATGCGGTACT GCTCTAGTGA TGGAACCAGT 
GGCTGCTCCA CCGACGGCCT GTCGGGCGAT TTCCTGCGGG CAGGGAGATA CTCCGCCCAC 
ACCGTTAACG GGGCGTGGTC GGCCTGGACG TCGTGGTCAC AGTGCAGCCG AGACTGCAGC 
CGGGGCATCC GGAACCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTT CGGGGGCATG 
CCTTGCCTGG GCCCGTCCCT GGAATACCAG GAATGCAACA TCTTGCCCTG CCCAGTGGAT 
GGCATGTGGT CTTGCTGGTC CTCCTGGTCC ACGTGCTCGG CAACGTGTGG AGGGGGACAC 
TACATGAGGA CACGCTCGTG CACAAACCCA GCCCCCGCCT ACGGAGGTGA CATCTGCCTG 
GGACTGCACA CAGAGGAGGC GCTCTGCAAC ACGCAGCCCT GCCCCGAGAG CTGGTCGGAG 
TGGTCAGAGT GGTCAGAGTG TGACGTGGCT GGAGTCCAGG TCCGTGTCCG CCAGTGCGTC 
CTTCTGTTCC CCGTGGGCAG CCAGTGTTCC GGAAACACCA CAGAAAGCCG GCCCTGCATT 
TTTGATTCCA ACTTCATTCC AGAAGTGTCT GTGGCCAGAT CCAGCAGCAT AGAAGAGAAA 
AGATGTGGGG AGTTCAACTT GTTCCACATG ATTGCCGTGG GGCTCAGCAG CTCCATCCTA 
GGCTGCCTTC TCACGCTGCT CGTCTACACC TACTGCCAGC GGTACCAGCA ACAATCCCAC 
GACGCCACCG TCATCCACCC TGTCGCACCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATTAACAAAC TGGACAAGTA CGACTCGGTG GAGGCCATCA AGGCGTTTAA CAAAAACAAC 
TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCACATC TCACCGGGAA GACCTATTCT 
AACGCCTACT TTACAGATCT CAATAACTAT GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SEMA5A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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