PFKM cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKM
Entrez Gene ID	506544
Full Name	phosphofructokinase, muscle
Synonyms	ATP-PFK,PFK-M
General protein information	Preferred Names phosphofructokinase, muscle Names ATP-dependent 6-phosphofructokinase, muscle type 6-phosphofructokinase type A 6-phosphofructokinase, muscle type PFK-A phosphofructo-1-kinase isozyme A phosphofructokinase 1 phosphofructokinase-M phosphohexokinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001075268.1	NP_001068736.1	ATP-dependent 6-phosphofructokinase, muscle type
XM_024991492.1	XP_024847260.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
XM_024991491.1	XP_024847259.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
XM_010805037.1	XP_010803339.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Syntenic assignment of human chromosome 1 homologous loci in the bovine.
Threadgill DS, Threadgill DW, Moll YD, Weiss JA, Zhang N, Davey HW, Wildeman AG, Womack JE
Genomics22(3)626-30(1994 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01496	NM_001075268.1 Latest version!	Bos taurus phosphofructokinase, muscle (PFKM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa19264	XM_010805037.1 Latest version!	Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa234232	XM_024991492.1 Latest version!	Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa234232	XM_024991491.1 Latest version!	Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKM ( NM_001075268.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068736.1 Bos taurus phosphofructokinase, muscle (PFKM), mRNA.

Price & Availability↑

CloneID	OBa01496
Clone ID Related Accession (Same CDS sequence)	NM_001075268.1
Accession Version	NM_001075268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Bos taurus(cattle)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122655.1. On or before Oct 4, 2006 this sequence version replaced XM_882672.1, XM_882678.1, XM_882687.1, XM_583005.2, XM_869501.1. ##Evidence-Data-START## Transcript exon combination :: BC122655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145459 [ECO:0000348] ##Evidence-Data-END##

ATGACCCATG AGCACCATGC AGCCAAAACC CTGGGAATCG GCAAAGCCAT CGCCGTGTTA 
ACCTCTGGTG GCGATGCCCA AGGTATGAAT GCTGCTGTCA GGGCTGTGGT TCGTGTTGGC 
ATCTATACTG GTGCCCGTGT CTTCTTTGTC CATGAGGGTT ATCAAGGCCT GGTAGATGGT 
GGAGATAACA TCCGGGAAGC CACATGGGAG AGTGTCTCGA TGATGCTTCA GCTGGGAGGC 
ACGGTGATTG GAAGCGCCCG GTGCAAGGAC TTTCGGGAAC GGGAAGGCCG GCTCCGAGCT 
GCCCACAACC TGGTGAAACG TGGGATCACC AACCTGTGTG TCATTGGGGG AGACGGCAGC 
CTGACTGGGG CCGACACCTT CCGCTCTGAG TGGAGTGACT TGTTGAGTGA CCTCCAGAAA 
TCAGGCAAGA TTACAGCAGA GGAGGCTACC AAGTCCAGCT ACCTGAACAT CGTGGGGCTG 
GTTGGCTCAA TTGACAATGA CTTCTGTGGC ACTGATATGA CCATTGGCAC CGACTCTGCC 
CTGCACCGGA TCATAGAGAT TGTAGATGCC ATCACTACTA CCGCTCAGAG CCACCAGAGG 
ACCTTTGTGT TGGAGGTGAT GGGCCGGCAC TGTGGATACC TGGCCCTTGT CACTTCCCTC 
TCCTGTGGGG CCGACTGGGT CTTTATTCCC GAGTGTCCAC CAGATGACGA CTGGGAGGAA 
CACCTTTGTC GCCGGCTCAG CGAGACAAGG AACCGTGGTT CTCGTCTCAA CATCATCATC 
GTAGCTGAGG GTGCGATTGA CAAGAATGGG AAACCAATCA CCTCTGAAGA CATCAAAAAC 
CTGGTGGTTA AGCGTCTGGG ATATGACACC CGAGTCACCG TCTTGGGGCA CGTGCAGCGG 
GGTGGGACAC CGTCGGCCTT TGACAGAATC CTGGGCAGCA GGATGGGTGT GGAAGCAGTA 
ATGGCACTTT TGGAGGGGAC CCCGGACACC CCAGCCTGTG TGGTGAGCCT CTCTGGTAAC 
CAGGCTGTGC GCCTGCCCCT CATGGAGTGT GTCCAGGTGA CCAAGGACGT GACCAGGGCC 
ATGGATGAAA GGAGATTCGA TGAGGCCTTG AAGCTGAGGG GCCGGAGCTT CATGAACAAC 
TGGGAGGTAT ACAAGCTTCT GGCTCACGTC AGACCCCCAA AGTCTAAGAG TGGCTCCCAC 
ACGGTGGCCG TGATGAACGT GGGAGCGCCG GCCGCAGGCA TGAACGCAGC CGTTCGCTCC 
ACTGTGAGAA TCGGCCTCAT CCAGGGCAAC CGAATGCTGG TGGTGCACGA TGGCTTCGAG 
GGCCTGGCCA AGGGTCAGAT CGAGGAAGCT GGATGGAGCT ACGTCGGGGG CTGGACTGGC 
CAAGGTGGTT CTAAACTTGG GACTAAAAGG ACTCTACCCA AGAAGAGCTT TGAACAGATC 
AGTGCCAACA TCACGAAGTT TAATATTCAG GGCCTTGTCA TTATCGGGGG CTTTGAGGCT 
TACACGGGGG GCCTGGAGCT GATGGAAGGC AGGAAGCAGT ATGATGAGCT CTGCATCCCC 
TTCGTGGTCA TCCCTGCCAC GGTCTCCAAC AATGTGCCCG GCTCGGACTT CAGCGTGGGG 
GCTGACACTG CCCTCAACAC CATCTGCATG ACGTGTGACC GCATCAAGCA GTCAGCGGCA 
GGCACCAAGC GCCGTGTGTT TATCATTGAA ACGATGGGCG GCTACTGTGG CTACCTGGCC 
ACCATGGCAG GCCTGGCAGC TGGGGCTGAT GCTGCGTATA TTTTTGAGGA CCCCTTCACC 
ATTCGAGACC TGCAGGTGAA TGTGGAGCAT CTGGTGCAGA AGATGAAAAC GACTGTGAAG 
AGGGGCCTGG TGTTAAGGAA CGAGAAGTGC AATGAGAACT ACACCACTGA CTTCATCTTC 
AACTTGTACT CTGAGGAGGG GAAGGGCATC TTCGACAGCA GGAAGAACGT GCTGGGCCAC 
ATGCAGCAGG GTGGGAGCCC AACTCCATTC GACAGGAATT TTGCCACTAA GATGGGTGCA 
AAGGCTATGA ACTGGATGTC AGGGAAAATC AAAGAGAGTT ACCGTAATGG GCGGATCTTT 
GCCAATTCCC CAGACTCGGG CTGTGTTCTG GGGATGCGTA AGAGGGCCCT GGTCTTCCAA 
CCAGTGACTG AGTTGAAGGA ACAGACGGAT TTTGAGCACC GCATCCCCAA GGAGCAGTGG 
TGGCTGAAGC TGAGGCCCAT CCTCAAGATC CTCGCCAAGT ACGAGATTGA CTTGGATACC 
TCAGAGCACG CCCACCTGGA GCACATCTCT CGGAAGCGGT CCGGAGAAGC TAACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068736.1
CDS	59..2398
Translation	MTHEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIYTGARVFFVHEGYQGLVDGGDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKSGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRNRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTRAMDERRFDEALKLRGRSFMNNWEVYKLLAHVRPPKSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRMLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEDPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANSPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEANV

Target ORF information:

RefSeq Version	NM_001075268.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphofructokinase, muscle (PFKM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075268.1

ORF Insert Sequence:

ATGACCCATG AGCACCATGC AGCCAAAACC CTGGGAATCG GCAAAGCCAT CGCCGTGTTA 
ACCTCTGGTG GCGATGCCCA AGGTATGAAT GCTGCTGTCA GGGCTGTGGT TCGTGTTGGC 
ATCTATACTG GTGCCCGTGT CTTCTTTGTC CATGAGGGTT ATCAAGGCCT GGTAGATGGT 
GGAGATAACA TCCGGGAAGC CACATGGGAG AGTGTCTCGA TGATGCTTCA GCTGGGAGGC 
ACGGTGATTG GAAGCGCCCG GTGCAAGGAC TTTCGGGAAC GGGAAGGCCG GCTCCGAGCT 
GCCCACAACC TGGTGAAACG TGGGATCACC AACCTGTGTG TCATTGGGGG AGACGGCAGC 
CTGACTGGGG CCGACACCTT CCGCTCTGAG TGGAGTGACT TGTTGAGTGA CCTCCAGAAA 
TCAGGCAAGA TTACAGCAGA GGAGGCTACC AAGTCCAGCT ACCTGAACAT CGTGGGGCTG 
GTTGGCTCAA TTGACAATGA CTTCTGTGGC ACTGATATGA CCATTGGCAC CGACTCTGCC 
CTGCACCGGA TCATAGAGAT TGTAGATGCC ATCACTACTA CCGCTCAGAG CCACCAGAGG 
ACCTTTGTGT TGGAGGTGAT GGGCCGGCAC TGTGGATACC TGGCCCTTGT CACTTCCCTC 
TCCTGTGGGG CCGACTGGGT CTTTATTCCC GAGTGTCCAC CAGATGACGA CTGGGAGGAA 
CACCTTTGTC GCCGGCTCAG CGAGACAAGG AACCGTGGTT CTCGTCTCAA CATCATCATC 
GTAGCTGAGG GTGCGATTGA CAAGAATGGG AAACCAATCA CCTCTGAAGA CATCAAAAAC 
CTGGTGGTTA AGCGTCTGGG ATATGACACC CGAGTCACCG TCTTGGGGCA CGTGCAGCGG 
GGTGGGACAC CGTCGGCCTT TGACAGAATC CTGGGCAGCA GGATGGGTGT GGAAGCAGTA 
ATGGCACTTT TGGAGGGGAC CCCGGACACC CCAGCCTGTG TGGTGAGCCT CTCTGGTAAC 
CAGGCTGTGC GCCTGCCCCT CATGGAGTGT GTCCAGGTGA CCAAGGACGT GACCAGGGCC 
ATGGATGAAA GGAGATTCGA TGAGGCCTTG AAGCTGAGGG GCCGGAGCTT CATGAACAAC 
TGGGAGGTAT ACAAGCTTCT GGCTCACGTC AGACCCCCAA AGTCTAAGAG TGGCTCCCAC 
ACGGTGGCCG TGATGAACGT GGGAGCGCCG GCCGCAGGCA TGAACGCAGC CGTTCGCTCC 
ACTGTGAGAA TCGGCCTCAT CCAGGGCAAC CGAATGCTGG TGGTGCACGA TGGCTTCGAG 
GGCCTGGCCA AGGGTCAGAT CGAGGAAGCT GGATGGAGCT ACGTCGGGGG CTGGACTGGC 
CAAGGTGGTT CTAAACTTGG GACTAAAAGG ACTCTACCCA AGAAGAGCTT TGAACAGATC 
AGTGCCAACA TCACGAAGTT TAATATTCAG GGCCTTGTCA TTATCGGGGG CTTTGAGGCT 
TACACGGGGG GCCTGGAGCT GATGGAAGGC AGGAAGCAGT ATGATGAGCT CTGCATCCCC 
TTCGTGGTCA TCCCTGCCAC GGTCTCCAAC AATGTGCCCG GCTCGGACTT CAGCGTGGGG 
GCTGACACTG CCCTCAACAC CATCTGCATG ACGTGTGACC GCATCAAGCA GTCAGCGGCA 
GGCACCAAGC GCCGTGTGTT TATCATTGAA ACGATGGGCG GCTACTGTGG CTACCTGGCC 
ACCATGGCAG GCCTGGCAGC TGGGGCTGAT GCTGCGTATA TTTTTGAGGA CCCCTTCACC 
ATTCGAGACC TGCAGGTGAA TGTGGAGCAT CTGGTGCAGA AGATGAAAAC GACTGTGAAG 
AGGGGCCTGG TGTTAAGGAA CGAGAAGTGC AATGAGAACT ACACCACTGA CTTCATCTTC 
AACTTGTACT CTGAGGAGGG GAAGGGCATC TTCGACAGCA GGAAGAACGT GCTGGGCCAC 
ATGCAGCAGG GTGGGAGCCC AACTCCATTC GACAGGAATT TTGCCACTAA GATGGGTGCA 
AAGGCTATGA ACTGGATGTC AGGGAAAATC AAAGAGAGTT ACCGTAATGG GCGGATCTTT 
GCCAATTCCC CAGACTCGGG CTGTGTTCTG GGGATGCGTA AGAGGGCCCT GGTCTTCCAA 
CCAGTGACTG AGTTGAAGGA ACAGACGGAT TTTGAGCACC GCATCCCCAA GGAGCAGTGG 
TGGCTGAAGC TGAGGCCCAT CCTCAAGATC CTCGCCAAGT ACGAGATTGA CTTGGATACC 
TCAGAGCACG CCCACCTGGA GCACATCTCT CGGAAGCGGT CCGGAGAAGC TAACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_010805037.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803339.1 Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19264
Clone ID Related Accession (Same CDS sequence)	XM_010805037.1
Accession Version	XM_010805037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTAAAG TGGAATTGCA TCTGAAATTT TTCATGTGTG TAACTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGAAGAA GCTTCAACTT CCAGCATGCT CATGCAAAAG 
CCACCATCTA GGACAGATGT CCTGAAGAGT CTAGATACCG TGGATGATCC AGACACTGTG 
GAAGGCATAC CCATTTTTGC AAATGAGTGG ATCATGACCC ATGAGCACCA TGCAGCCAAA 
ACCCTGGGAA TCGGCAAAGC CATCGCCGTG TTAACCTCTG GTGGCGATGC CCAAGGTATG 
AATGCTGCTG TCAGGGCTGT GGTTCGTGTT GGCATCTATA CTGGTGCCCG TGTCTTCTTT 
GTCCATGAGG GTTATCAAGG CCTGGTAGAT GGTGGAGATA ACATCCGGGA AGCCACATGG 
GAGAGTGTCT CGATGATGCT TCAGCTGGGA GGCACGGTGA TTGGAAGCGC CCGGTGCAAG 
GACTTTCGGG AACGGGAAGG CCGGCTCCGA GCTGCCCACA ACCTGGTGAA ACGTGGGATC 
ACCAACCTGT GTGTCATTGG GGGAGACGGC AGCCTGACTG GGGCCGACAC CTTCCGCTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAATCAGGCA AGATTACAGC AGAGGAGGCT 
ACCAAGTCCA GCTACCTGAA CATCGTGGGG CTGGTTGGCT CAATTGACAA TGACTTCTGT 
GGCACTGATA TGACCATTGG CACCGACTCT GCCCTGCACC GGATCATAGA GATTGTAGAT 
GCCATCACTA CTACCGCTCA GAGCCACCAG AGGACCTTTG TGTTGGAGGT GATGGGCCGG 
CACTGTGGAT ACCTGGCCCT TGTCACTTCC CTCTCCTGTG GGGCCGACTG GGTCTTTATT 
CCCGAGTGTC CACCAGATGA CGACTGGGAG GAACACCTTT GTCGCCGGCT CAGCGAGACA 
AGGAACCGTG GTTCTCGTCT CAACATCATC ATCGTAGCTG AGGGTGCGAT TGACAAGAAT 
GGGAAACCAA TCACCTCTGA AGACATCAAA AACCTGGTGG TTAAGCGTCT GGGATATGAC 
ACCCGAGTCA CCGTCTTGGG GCACGTGCAG CGGGGTGGGA CACCGTCGGC CTTTGACAGA 
ATCCTGGGCA GCAGGATGGG TGTGGAAGCA GTAATGGCAC TTTTGGAGGG GACCCCGGAC 
ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT AACCAGGCTG TGCGCCTGCC CCTCATGGAG 
TGTGTCCAGG TGACCAAGGA CGTGACCAGG GCCATGGATG AAAGGAGATT CGATGAGGCC 
TTGAAGCTGA GGGGCCGGAG CTTCATGAAC AACTGGGAGG TATACAAGCT TCTGGCTCAC 
GTCAGACCCC CAAAGTCTAA GAGTGGCTCC CACACGGTGG CCGTGATGAA CGTGGGAGCG 
CCGGCCGCAG GCATGAACGC AGCCGTTCGC TCCACTGTGA GAATCGGCCT CATCCAGGGC 
AACCGAATGC TGGTGGTGCA CGATGGCTTC GAGGGCCTGG CCAAGGGTCA GATCGAGGAA 
GCTGGATGGA GCTACGTCGG GGGCTGGACT GGCCAAGGTG GTTCTAAACT TGGGACTAAA 
AGGACTCTAC CCAAGAAGAG CTTTGAACAG ATCAGTGCCA ACATCACGAA GTTTAATATT 
CAGGGCCTTG TCATTATCGG GGGCTTTGAG GCTTACACGG GGGGCCTGGA GCTGATGGAA 
GGCAGGAAGC AGTATGATGA GCTCTGCATC CCCTTCGTGG TCATCCCTGC CACGGTCTCC 
AACAATGTGC CCGGCTCGGA CTTCAGCGTG GGGGCTGACA CTGCCCTCAA CACCATCTGC 
ATGACGTGTG ACCGCATCAA GCAGTCAGCG GCAGGCACCA AGCGCCGTGT GTTTATCATT 
GAAACGATGG GCGGCTACTG TGGCTACCTG GCCACCATGG CAGGCCTGGC AGCTGGGGCT 
GATGCTGCGT ATATTTTTGA GGACCCCTTC ACCATTCGAG ACCTGCAGGT GAATGTGGAG 
CATCTGGTGC AGAAGATGAA AACGACTGTG AAGAGGGGCC TGGTGTTAAG GAACGAGAAG 
TGCAATGAGA ACTACACCAC TGACTTCATC TTCAACTTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA CGTGCTGGGC CACATGCAGC AGGGTGGGAG CCCAACTCCA 
TTTGACAGGA ATTTTGCCAC TAAGATGGGT GCAAAGGCTA TGAACTGGAT GTCAGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATT CCCCAGACTC GGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC CCTGGTCTTC CAACCAGTGA CTGAGTTGAA GGAACAGACG 
GATTTTGAGC ACCGCATCCC CAAGGAGCAG TGGTGGCTGA AGCTGAGGCC CATCCTCAAG 
ATCCTCGCCA AGTACGAGAT TGACTTGGAT ACCTCAGAGC ACGCCCACCT GGAGCACATC 
TCTCGGAAGC GGTCCGGAGA AGCTAACGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803339.1
CDS	16..2568
Translation	MRKVELHLKFFMCVTQSRQLVRTPQRTAEEASTSSMLMQKPPSRTDVLKSLDTVDDPDTVEGIPIFANEWIMTHEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIYTGARVFFVHEGYQGLVDGGDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKSGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRNRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTRAMDERRFDEALKLRGRSFMNNWEVYKLLAHVRPPKSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRMLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEDPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANSPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEANV

Target ORF information:

RefSeq Version	XM_010805037.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805037.1

ORF Insert Sequence:

ATGCGTAAAG TGGAATTGCA TCTGAAATTT TTCATGTGTG TAACTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGAAGAA GCTTCAACTT CCAGCATGCT CATGCAAAAG 
CCACCATCTA GGACAGATGT CCTGAAGAGT CTAGATACCG TGGATGATCC AGACACTGTG 
GAAGGCATAC CCATTTTTGC AAATGAGTGG ATCATGACCC ATGAGCACCA TGCAGCCAAA 
ACCCTGGGAA TCGGCAAAGC CATCGCCGTG TTAACCTCTG GTGGCGATGC CCAAGGTATG 
AATGCTGCTG TCAGGGCTGT GGTTCGTGTT GGCATCTATA CTGGTGCCCG TGTCTTCTTT 
GTCCATGAGG GTTATCAAGG CCTGGTAGAT GGTGGAGATA ACATCCGGGA AGCCACATGG 
GAGAGTGTCT CGATGATGCT TCAGCTGGGA GGCACGGTGA TTGGAAGCGC CCGGTGCAAG 
GACTTTCGGG AACGGGAAGG CCGGCTCCGA GCTGCCCACA ACCTGGTGAA ACGTGGGATC 
ACCAACCTGT GTGTCATTGG GGGAGACGGC AGCCTGACTG GGGCCGACAC CTTCCGCTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAATCAGGCA AGATTACAGC AGAGGAGGCT 
ACCAAGTCCA GCTACCTGAA CATCGTGGGG CTGGTTGGCT CAATTGACAA TGACTTCTGT 
GGCACTGATA TGACCATTGG CACCGACTCT GCCCTGCACC GGATCATAGA GATTGTAGAT 
GCCATCACTA CTACCGCTCA GAGCCACCAG AGGACCTTTG TGTTGGAGGT GATGGGCCGG 
CACTGTGGAT ACCTGGCCCT TGTCACTTCC CTCTCCTGTG GGGCCGACTG GGTCTTTATT 
CCCGAGTGTC CACCAGATGA CGACTGGGAG GAACACCTTT GTCGCCGGCT CAGCGAGACA 
AGGAACCGTG GTTCTCGTCT CAACATCATC ATCGTAGCTG AGGGTGCGAT TGACAAGAAT 
GGGAAACCAA TCACCTCTGA AGACATCAAA AACCTGGTGG TTAAGCGTCT GGGATATGAC 
ACCCGAGTCA CCGTCTTGGG GCACGTGCAG CGGGGTGGGA CACCGTCGGC CTTTGACAGA 
ATCCTGGGCA GCAGGATGGG TGTGGAAGCA GTAATGGCAC TTTTGGAGGG GACCCCGGAC 
ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT AACCAGGCTG TGCGCCTGCC CCTCATGGAG 
TGTGTCCAGG TGACCAAGGA CGTGACCAGG GCCATGGATG AAAGGAGATT CGATGAGGCC 
TTGAAGCTGA GGGGCCGGAG CTTCATGAAC AACTGGGAGG TATACAAGCT TCTGGCTCAC 
GTCAGACCCC CAAAGTCTAA GAGTGGCTCC CACACGGTGG CCGTGATGAA CGTGGGAGCG 
CCGGCCGCAG GCATGAACGC AGCCGTTCGC TCCACTGTGA GAATCGGCCT CATCCAGGGC 
AACCGAATGC TGGTGGTGCA CGATGGCTTC GAGGGCCTGG CCAAGGGTCA GATCGAGGAA 
GCTGGATGGA GCTACGTCGG GGGCTGGACT GGCCAAGGTG GTTCTAAACT TGGGACTAAA 
AGGACTCTAC CCAAGAAGAG CTTTGAACAG ATCAGTGCCA ACATCACGAA GTTTAATATT 
CAGGGCCTTG TCATTATCGG GGGCTTTGAG GCTTACACGG GGGGCCTGGA GCTGATGGAA 
GGCAGGAAGC AGTATGATGA GCTCTGCATC CCCTTCGTGG TCATCCCTGC CACGGTCTCC 
AACAATGTGC CCGGCTCGGA CTTCAGCGTG GGGGCTGACA CTGCCCTCAA CACCATCTGC 
ATGACGTGTG ACCGCATCAA GCAGTCAGCG GCAGGCACCA AGCGCCGTGT GTTTATCATT 
GAAACGATGG GCGGCTACTG TGGCTACCTG GCCACCATGG CAGGCCTGGC AGCTGGGGCT 
GATGCTGCGT ATATTTTTGA GGACCCCTTC ACCATTCGAG ACCTGCAGGT GAATGTGGAG 
CATCTGGTGC AGAAGATGAA AACGACTGTG AAGAGGGGCC TGGTGTTAAG GAACGAGAAG 
TGCAATGAGA ACTACACCAC TGACTTCATC TTCAACTTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA CGTGCTGGGC CACATGCAGC AGGGTGGGAG CCCAACTCCA 
TTTGACAGGA ATTTTGCCAC TAAGATGGGT GCAAAGGCTA TGAACTGGAT GTCAGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATT CCCCAGACTC GGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC CCTGGTCTTC CAACCAGTGA CTGAGTTGAA GGAACAGACG 
GATTTTGAGC ACCGCATCCC CAAGGAGCAG TGGTGGCTGA AGCTGAGGCC CATCCTCAAG 
ATCCTCGCCA AGTACGAGAT TGACTTGGAT ACCTCAGAGC ACGCCCACCT GGAGCACATC 
TCTCGGAAGC GGTCCGGAGA AGCTAACGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_024991492.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847260.1 Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa234232
Clone ID Related Accession (Same CDS sequence)	XM_024991492.1 , XM_024991491.1
Accession Version	XM_024991492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AGCACCATGC AGCCAAAACC CTGGGAATCG GCAAAGCCAT CGCCGTGTTA 
ACCTCTGGTG GCGATGCCCA AGGTATGAAT GCTGCTGTCA GGGCTGTGGT TCGTGTTGGC 
ATCTATACTG GTGCCCGTGT CTTCTTTGTC CATGAGGGTT ATCAAGGCCT GGTAGATGGT 
GGAGATAACA TCCGGGAAGC CACATGGGAG AGTGTCTCGA TGATGCTTCA GCTGGGAGGC 
ACGGTGATTG GAAGCGCCCG GTGCAAGGAC TTTCGGGAAC GGGAAGGCCG GCTCCGAGCT 
GCCCACAACC TGGTGAAACG TGGGATCACC AACCTGTGTG TCATTGGGGG AGACGGCAGC 
CTGACTGGGG CCGACACCTT CCGCTCTGAG TGGAGTGACT TGTTGAGTGA CCTCCAGAAA 
TCAGGCAAGA TTACAGCAGA GGAGGCTACC AAGTCCAGCT ACCTGAACAT CGTGGGGCTG 
GTTGGCTCAA TTGACAATGA CTTCTGTGGC ACTGATATGA CCATTGGCAC CGACTCTGCC 
CTGCACCGGA TCATAGAGAT TGTAGATGCC ATCACTACTA CCGCTCAGAG CCACCAGAGG 
ACCTTTGTGT TGGAGGTGAT GGGCCGGCAC TGTGGATACC TGGCCCTTGT CACTTCCCTC 
TCCTGTGGGG CCGACTGGGT CTTTATTCCC GAGTGTCCAC CAGATGACGA CTGGGAGGAA 
CACCTTTGTC GCCGGCTCAG CGAGACAAGG AACCGTGGTT CTCGTCTCAA CATCATCATC 
GTAGCTGAGG GTGCGATTGA CAAGAATGGG AAACCAATCA CCTCTGAAGA CATCAAAAAC 
CTGGTGGTTA AGCGTCTGGG ATATGACACC CGAGTCACCG TCTTGGGGCA CGTGCAGCGG 
GGTGGGACAC CGTCGGCCTT TGACAGAATC CTGGGCAGCA GGATGGGTGT GGAAGCAGTA 
ATGGCACTTT TGGAGGGGAC CCCGGACACC CCAGCCTGTG TGGTGAGCCT CTCTGGTAAC 
CAGGCTGTGC GCCTGCCCCT CATGGAGTGT GTCCAGGTGA CCAAGGACGT GACCAGGGCC 
ATGGATGAAA GGAGATTCGA TGAGGCCTTG AAGCTGAGGG GCCGGAGCTT CATGAACAAC 
TGGGAGGTAT ACAAGCTTCT GGCTCACGTC AGACCCCCAA AGTCTAAGAG TGGCTCCCAC 
ACGGTGGCCG TGATGAACGT GGGAGCGCCG GCCGCAGGCA TGAACGCAGC CGTTCGCTCC 
ACTGTGAGAA TCGGCCTCAT CCAGGGCAAC CGAATGCTGG TGGTGCACGA TGGCTTCGAG 
GGCCTGGCCA AGGGTCAGAT CGAGGAAGCT GGATGGAGCT ACGTCGGGGG CTGGACTGGC 
CAAGGTGGTT CTAAACTTGG GACTAAAAGG ACTCTACCCA AGAAGAGCTT TGAACAGATC 
AGTGCCAACA TCACGAAGTT TAATATTCAG GGCCTTGTCA TTATCGGGGG CTTTGAGGCT 
TACACGGGGG GCCTGGAGCT GATGGAAGGC AGGAAGCAGT ATGATGAGCT CTGCATCCCC 
TTCGTGGTCA TCCCTGCCAC GGTCTCCAAC AATGTGCCCG GCTCGGACTT CAGCGTGGGG 
GCTGACACTG CCCTCAACAC CATCTGCATG ACGTGTGACC GCATCAAGCA GTCAGCGGCA 
GGCACCAAGC GCCGTGTGTT TATCATTGAA ACGATGGGCG GCTACTGTGG CTACCTGGCC 
ACCATGGCAG GCCTGGCAGC TGGGGCTGAT GCTGCGTATA TTTTTGAGGA CCCCTTCACC 
ATTCGAGACC TGCAGGTGAA TGTGGAGCAT CTGGTGCAGA AGATGAAAAC GACTGTGAAG 
AGGGGCCTGG TGTTAAGGAA CGAGAAGTGC AATGAGAACT ACACCACTGA CTTCATCTTC 
AACTTGTACT CTGAGGAGGG GAAGGGCATC TTCGACAGCA GGAAGAACGT GCTGGGCCAC 
ATGCAGCAGG GTGGGAGCCC AACTCCATTT GACAGGAATT TTGCCACTAA GATGGGTGCA 
AAGGCTATGA ACTGGATGTC AGGGAAAATC AAAGAGAGTT ACCGTAATGG GCGGATCTTT 
GCCAATTCCC CAGACTCGGG CTGTGTTCTG GGGATGCGTA AGAGGGCCCT GGTCTTCCAA 
CCAGTGACTG AGTTGAAGGA ACAGACGGAT TTTGAGCACC GCATCCCCAA GGAGCAGTGG 
TGGCTGAAGC TGAGGCCCAT CCTCAAGATC CTCGCCAAGT ACGAGATTGA CTTGGATACC 
TCAGAGCACG CCCACCTGGA GCACATCTCT CGGAAGCGGT CCGGAGAAGC TAACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847260.1
CDS	172..2511
Translation	MTHEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIYTGARVFFVHEGYQGLVDGGDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKSGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRNRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTRAMDERRFDEALKLRGRSFMNNWEVYKLLAHVRPPKSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRMLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEDPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANSPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEANV

Target ORF information:

RefSeq Version	XM_024991492.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991492.1

ORF Insert Sequence:

ATGACCCATG AGCACCATGC AGCCAAAACC CTGGGAATCG GCAAAGCCAT CGCCGTGTTA 
ACCTCTGGTG GCGATGCCCA AGGTATGAAT GCTGCTGTCA GGGCTGTGGT TCGTGTTGGC 
ATCTATACTG GTGCCCGTGT CTTCTTTGTC CATGAGGGTT ATCAAGGCCT GGTAGATGGT 
GGAGATAACA TCCGGGAAGC CACATGGGAG AGTGTCTCGA TGATGCTTCA GCTGGGAGGC 
ACGGTGATTG GAAGCGCCCG GTGCAAGGAC TTTCGGGAAC GGGAAGGCCG GCTCCGAGCT 
GCCCACAACC TGGTGAAACG TGGGATCACC AACCTGTGTG TCATTGGGGG AGACGGCAGC 
CTGACTGGGG CCGACACCTT CCGCTCTGAG TGGAGTGACT TGTTGAGTGA CCTCCAGAAA 
TCAGGCAAGA TTACAGCAGA GGAGGCTACC AAGTCCAGCT ACCTGAACAT CGTGGGGCTG 
GTTGGCTCAA TTGACAATGA CTTCTGTGGC ACTGATATGA CCATTGGCAC CGACTCTGCC 
CTGCACCGGA TCATAGAGAT TGTAGATGCC ATCACTACTA CCGCTCAGAG CCACCAGAGG 
ACCTTTGTGT TGGAGGTGAT GGGCCGGCAC TGTGGATACC TGGCCCTTGT CACTTCCCTC 
TCCTGTGGGG CCGACTGGGT CTTTATTCCC GAGTGTCCAC CAGATGACGA CTGGGAGGAA 
CACCTTTGTC GCCGGCTCAG CGAGACAAGG AACCGTGGTT CTCGTCTCAA CATCATCATC 
GTAGCTGAGG GTGCGATTGA CAAGAATGGG AAACCAATCA CCTCTGAAGA CATCAAAAAC 
CTGGTGGTTA AGCGTCTGGG ATATGACACC CGAGTCACCG TCTTGGGGCA CGTGCAGCGG 
GGTGGGACAC CGTCGGCCTT TGACAGAATC CTGGGCAGCA GGATGGGTGT GGAAGCAGTA 
ATGGCACTTT TGGAGGGGAC CCCGGACACC CCAGCCTGTG TGGTGAGCCT CTCTGGTAAC 
CAGGCTGTGC GCCTGCCCCT CATGGAGTGT GTCCAGGTGA CCAAGGACGT GACCAGGGCC 
ATGGATGAAA GGAGATTCGA TGAGGCCTTG AAGCTGAGGG GCCGGAGCTT CATGAACAAC 
TGGGAGGTAT ACAAGCTTCT GGCTCACGTC AGACCCCCAA AGTCTAAGAG TGGCTCCCAC 
ACGGTGGCCG TGATGAACGT GGGAGCGCCG GCCGCAGGCA TGAACGCAGC CGTTCGCTCC 
ACTGTGAGAA TCGGCCTCAT CCAGGGCAAC CGAATGCTGG TGGTGCACGA TGGCTTCGAG 
GGCCTGGCCA AGGGTCAGAT CGAGGAAGCT GGATGGAGCT ACGTCGGGGG CTGGACTGGC 
CAAGGTGGTT CTAAACTTGG GACTAAAAGG ACTCTACCCA AGAAGAGCTT TGAACAGATC 
AGTGCCAACA TCACGAAGTT TAATATTCAG GGCCTTGTCA TTATCGGGGG CTTTGAGGCT 
TACACGGGGG GCCTGGAGCT GATGGAAGGC AGGAAGCAGT ATGATGAGCT CTGCATCCCC 
TTCGTGGTCA TCCCTGCCAC GGTCTCCAAC AATGTGCCCG GCTCGGACTT CAGCGTGGGG 
GCTGACACTG CCCTCAACAC CATCTGCATG ACGTGTGACC GCATCAAGCA GTCAGCGGCA 
GGCACCAAGC GCCGTGTGTT TATCATTGAA ACGATGGGCG GCTACTGTGG CTACCTGGCC 
ACCATGGCAG GCCTGGCAGC TGGGGCTGAT GCTGCGTATA TTTTTGAGGA CCCCTTCACC 
ATTCGAGACC TGCAGGTGAA TGTGGAGCAT CTGGTGCAGA AGATGAAAAC GACTGTGAAG 
AGGGGCCTGG TGTTAAGGAA CGAGAAGTGC AATGAGAACT ACACCACTGA CTTCATCTTC 
AACTTGTACT CTGAGGAGGG GAAGGGCATC TTCGACAGCA GGAAGAACGT GCTGGGCCAC 
ATGCAGCAGG GTGGGAGCCC AACTCCATTT GACAGGAATT TTGCCACTAA GATGGGTGCA 
AAGGCTATGA ACTGGATGTC AGGGAAAATC AAAGAGAGTT ACCGTAATGG GCGGATCTTT 
GCCAATTCCC CAGACTCGGG CTGTGTTCTG GGGATGCGTA AGAGGGCCCT GGTCTTCCAA 
CCAGTGACTG AGTTGAAGGA ACAGACGGAT TTTGAGCACC GCATCCCCAA GGAGCAGTGG 
TGGCTGAAGC TGAGGCCCAT CCTCAAGATC CTCGCCAAGT ACGAGATTGA CTTGGATACC 
TCAGAGCACG CCCACCTGGA GCACATCTCT CGGAAGCGGT CCGGAGAAGC TAACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_024991491.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847259.1 Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa234232
Clone ID Related Accession (Same CDS sequence)	XM_024991492.1 , XM_024991491.1
Accession Version	XM_024991491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AGCACCATGC AGCCAAAACC CTGGGAATCG GCAAAGCCAT CGCCGTGTTA 
ACCTCTGGTG GCGATGCCCA AGGTATGAAT GCTGCTGTCA GGGCTGTGGT TCGTGTTGGC 
ATCTATACTG GTGCCCGTGT CTTCTTTGTC CATGAGGGTT ATCAAGGCCT GGTAGATGGT 
GGAGATAACA TCCGGGAAGC CACATGGGAG AGTGTCTCGA TGATGCTTCA GCTGGGAGGC 
ACGGTGATTG GAAGCGCCCG GTGCAAGGAC TTTCGGGAAC GGGAAGGCCG GCTCCGAGCT 
GCCCACAACC TGGTGAAACG TGGGATCACC AACCTGTGTG TCATTGGGGG AGACGGCAGC 
CTGACTGGGG CCGACACCTT CCGCTCTGAG TGGAGTGACT TGTTGAGTGA CCTCCAGAAA 
TCAGGCAAGA TTACAGCAGA GGAGGCTACC AAGTCCAGCT ACCTGAACAT CGTGGGGCTG 
GTTGGCTCAA TTGACAATGA CTTCTGTGGC ACTGATATGA CCATTGGCAC CGACTCTGCC 
CTGCACCGGA TCATAGAGAT TGTAGATGCC ATCACTACTA CCGCTCAGAG CCACCAGAGG 
ACCTTTGTGT TGGAGGTGAT GGGCCGGCAC TGTGGATACC TGGCCCTTGT CACTTCCCTC 
TCCTGTGGGG CCGACTGGGT CTTTATTCCC GAGTGTCCAC CAGATGACGA CTGGGAGGAA 
CACCTTTGTC GCCGGCTCAG CGAGACAAGG AACCGTGGTT CTCGTCTCAA CATCATCATC 
GTAGCTGAGG GTGCGATTGA CAAGAATGGG AAACCAATCA CCTCTGAAGA CATCAAAAAC 
CTGGTGGTTA AGCGTCTGGG ATATGACACC CGAGTCACCG TCTTGGGGCA CGTGCAGCGG 
GGTGGGACAC CGTCGGCCTT TGACAGAATC CTGGGCAGCA GGATGGGTGT GGAAGCAGTA 
ATGGCACTTT TGGAGGGGAC CCCGGACACC CCAGCCTGTG TGGTGAGCCT CTCTGGTAAC 
CAGGCTGTGC GCCTGCCCCT CATGGAGTGT GTCCAGGTGA CCAAGGACGT GACCAGGGCC 
ATGGATGAAA GGAGATTCGA TGAGGCCTTG AAGCTGAGGG GCCGGAGCTT CATGAACAAC 
TGGGAGGTAT ACAAGCTTCT GGCTCACGTC AGACCCCCAA AGTCTAAGAG TGGCTCCCAC 
ACGGTGGCCG TGATGAACGT GGGAGCGCCG GCCGCAGGCA TGAACGCAGC CGTTCGCTCC 
ACTGTGAGAA TCGGCCTCAT CCAGGGCAAC CGAATGCTGG TGGTGCACGA TGGCTTCGAG 
GGCCTGGCCA AGGGTCAGAT CGAGGAAGCT GGATGGAGCT ACGTCGGGGG CTGGACTGGC 
CAAGGTGGTT CTAAACTTGG GACTAAAAGG ACTCTACCCA AGAAGAGCTT TGAACAGATC 
AGTGCCAACA TCACGAAGTT TAATATTCAG GGCCTTGTCA TTATCGGGGG CTTTGAGGCT 
TACACGGGGG GCCTGGAGCT GATGGAAGGC AGGAAGCAGT ATGATGAGCT CTGCATCCCC 
TTCGTGGTCA TCCCTGCCAC GGTCTCCAAC AATGTGCCCG GCTCGGACTT CAGCGTGGGG 
GCTGACACTG CCCTCAACAC CATCTGCATG ACGTGTGACC GCATCAAGCA GTCAGCGGCA 
GGCACCAAGC GCCGTGTGTT TATCATTGAA ACGATGGGCG GCTACTGTGG CTACCTGGCC 
ACCATGGCAG GCCTGGCAGC TGGGGCTGAT GCTGCGTATA TTTTTGAGGA CCCCTTCACC 
ATTCGAGACC TGCAGGTGAA TGTGGAGCAT CTGGTGCAGA AGATGAAAAC GACTGTGAAG 
AGGGGCCTGG TGTTAAGGAA CGAGAAGTGC AATGAGAACT ACACCACTGA CTTCATCTTC 
AACTTGTACT CTGAGGAGGG GAAGGGCATC TTCGACAGCA GGAAGAACGT GCTGGGCCAC 
ATGCAGCAGG GTGGGAGCCC AACTCCATTT GACAGGAATT TTGCCACTAA GATGGGTGCA 
AAGGCTATGA ACTGGATGTC AGGGAAAATC AAAGAGAGTT ACCGTAATGG GCGGATCTTT 
GCCAATTCCC CAGACTCGGG CTGTGTTCTG GGGATGCGTA AGAGGGCCCT GGTCTTCCAA 
CCAGTGACTG AGTTGAAGGA ACAGACGGAT TTTGAGCACC GCATCCCCAA GGAGCAGTGG 
TGGCTGAAGC TGAGGCCCAT CCTCAAGATC CTCGCCAAGT ACGAGATTGA CTTGGATACC 
TCAGAGCACG CCCACCTGGA GCACATCTCT CGGAAGCGGT CCGGAGAAGC TAACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847259.1
CDS	336..2675
Translation	MTHEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIYTGARVFFVHEGYQGLVDGGDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKSGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRNRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTRAMDERRFDEALKLRGRSFMNNWEVYKLLAHVRPPKSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRMLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEDPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANSPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEANV

Target ORF information:

RefSeq Version	XM_024991491.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991491.1

ORF Insert Sequence:

ATGACCCATG AGCACCATGC AGCCAAAACC CTGGGAATCG GCAAAGCCAT CGCCGTGTTA 
ACCTCTGGTG GCGATGCCCA AGGTATGAAT GCTGCTGTCA GGGCTGTGGT TCGTGTTGGC 
ATCTATACTG GTGCCCGTGT CTTCTTTGTC CATGAGGGTT ATCAAGGCCT GGTAGATGGT 
GGAGATAACA TCCGGGAAGC CACATGGGAG AGTGTCTCGA TGATGCTTCA GCTGGGAGGC 
ACGGTGATTG GAAGCGCCCG GTGCAAGGAC TTTCGGGAAC GGGAAGGCCG GCTCCGAGCT 
GCCCACAACC TGGTGAAACG TGGGATCACC AACCTGTGTG TCATTGGGGG AGACGGCAGC 
CTGACTGGGG CCGACACCTT CCGCTCTGAG TGGAGTGACT TGTTGAGTGA CCTCCAGAAA 
TCAGGCAAGA TTACAGCAGA GGAGGCTACC AAGTCCAGCT ACCTGAACAT CGTGGGGCTG 
GTTGGCTCAA TTGACAATGA CTTCTGTGGC ACTGATATGA CCATTGGCAC CGACTCTGCC 
CTGCACCGGA TCATAGAGAT TGTAGATGCC ATCACTACTA CCGCTCAGAG CCACCAGAGG 
ACCTTTGTGT TGGAGGTGAT GGGCCGGCAC TGTGGATACC TGGCCCTTGT CACTTCCCTC 
TCCTGTGGGG CCGACTGGGT CTTTATTCCC GAGTGTCCAC CAGATGACGA CTGGGAGGAA 
CACCTTTGTC GCCGGCTCAG CGAGACAAGG AACCGTGGTT CTCGTCTCAA CATCATCATC 
GTAGCTGAGG GTGCGATTGA CAAGAATGGG AAACCAATCA CCTCTGAAGA CATCAAAAAC 
CTGGTGGTTA AGCGTCTGGG ATATGACACC CGAGTCACCG TCTTGGGGCA CGTGCAGCGG 
GGTGGGACAC CGTCGGCCTT TGACAGAATC CTGGGCAGCA GGATGGGTGT GGAAGCAGTA 
ATGGCACTTT TGGAGGGGAC CCCGGACACC CCAGCCTGTG TGGTGAGCCT CTCTGGTAAC 
CAGGCTGTGC GCCTGCCCCT CATGGAGTGT GTCCAGGTGA CCAAGGACGT GACCAGGGCC 
ATGGATGAAA GGAGATTCGA TGAGGCCTTG AAGCTGAGGG GCCGGAGCTT CATGAACAAC 
TGGGAGGTAT ACAAGCTTCT GGCTCACGTC AGACCCCCAA AGTCTAAGAG TGGCTCCCAC 
ACGGTGGCCG TGATGAACGT GGGAGCGCCG GCCGCAGGCA TGAACGCAGC CGTTCGCTCC 
ACTGTGAGAA TCGGCCTCAT CCAGGGCAAC CGAATGCTGG TGGTGCACGA TGGCTTCGAG 
GGCCTGGCCA AGGGTCAGAT CGAGGAAGCT GGATGGAGCT ACGTCGGGGG CTGGACTGGC 
CAAGGTGGTT CTAAACTTGG GACTAAAAGG ACTCTACCCA AGAAGAGCTT TGAACAGATC 
AGTGCCAACA TCACGAAGTT TAATATTCAG GGCCTTGTCA TTATCGGGGG CTTTGAGGCT 
TACACGGGGG GCCTGGAGCT GATGGAAGGC AGGAAGCAGT ATGATGAGCT CTGCATCCCC 
TTCGTGGTCA TCCCTGCCAC GGTCTCCAAC AATGTGCCCG GCTCGGACTT CAGCGTGGGG 
GCTGACACTG CCCTCAACAC CATCTGCATG ACGTGTGACC GCATCAAGCA GTCAGCGGCA 
GGCACCAAGC GCCGTGTGTT TATCATTGAA ACGATGGGCG GCTACTGTGG CTACCTGGCC 
ACCATGGCAG GCCTGGCAGC TGGGGCTGAT GCTGCGTATA TTTTTGAGGA CCCCTTCACC 
ATTCGAGACC TGCAGGTGAA TGTGGAGCAT CTGGTGCAGA AGATGAAAAC GACTGTGAAG 
AGGGGCCTGG TGTTAAGGAA CGAGAAGTGC AATGAGAACT ACACCACTGA CTTCATCTTC 
AACTTGTACT CTGAGGAGGG GAAGGGCATC TTCGACAGCA GGAAGAACGT GCTGGGCCAC 
ATGCAGCAGG GTGGGAGCCC AACTCCATTT GACAGGAATT TTGCCACTAA GATGGGTGCA 
AAGGCTATGA ACTGGATGTC AGGGAAAATC AAAGAGAGTT ACCGTAATGG GCGGATCTTT 
GCCAATTCCC CAGACTCGGG CTGTGTTCTG GGGATGCGTA AGAGGGCCCT GGTCTTCCAA 
CCAGTGACTG AGTTGAAGGA ACAGACGGAT TTTGAGCACC GCATCCCCAA GGAGCAGTGG 
TGGCTGAAGC TGAGGCCCAT CCTCAAGATC CTCGCCAAGT ACGAGATTGA CTTGGATACC 
TCAGAGCACG CCCACCTGGA GCACATCTCT CGGAAGCGGT CCGGAGAAGC TAACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Syntenic assignment of human chromosome 1 homologous loci in the bovine.
Genomics22(3)626-30(1994 Aug)
Threadgill DS,Threadgill DW,Moll YD,Weiss JA,Zhang N,Davey HW,Wildeman AG,Womack JE

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PFKM cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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