WNK2 cDNA ORF clone, Bos taurus(cattle)

The following WNK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237499 XM_002689849.6
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa237500 XM_005210446.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa237501 XM_005210447.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa237502 XM_005210448.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa237503 XM_005210449.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa237504 XM_005210450.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa237505 XM_005210451.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa22296 XM_582977.9
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22296 XM_002689849.5
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22297 XM_005210446.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22297 XM_005199273.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22298 XM_005199274.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22298 XM_005210447.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22299 XM_005199275.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22299 XM_005210448.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22300 XM_005210449.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22300 XM_005199276.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22301 XM_005210450.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22301 XM_005199277.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22302 XM_005210451.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa22302 XM_005199278.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237499
    Clone ID Related Accession (Same CDS sequence) XM_002689849.6
    Accession Version XM_002689849.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689849.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCTGCCT TGAAGCGTCT CTGTCTAGGC AAAGAGCACA GCAGTCGGTC CTCCACCAGC
    AGCCTGGTCC CGGGCCCTGA GCCGGGCCCC CAACCTGCCC TGCACATCCA GGCGCAGGTG
    AACAACAGCA ACAACAAGAA AGGCACCTTC ACAGACGATC TGCACAAGCT GGTGGACGAG
    TGGACAAGCA AGACGGTCGG GGCCACACCA CTAAAGCCGT CACTCAACCA GTTGAAGCAG
    ACGCAGAAAT TGCATGACAT GGAGGCCACA GCAGGCTGGC CCCTGGCCTC TGGCGAGGCA
    CGGGCTATGA ACACATCTCG AACGGCAATG GGGATGCCAT GTCTGGCCCC GGTACCCAGC
    CCTGTACCCA CCGTGGTCAT TCCTGGAGCT GCCTCAGCCC TTCCTGTGCC CATGCCAGAT
    TCTGAAAGTG AGAAGCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689895.4
    CDS207..6710
    Translation

    Target ORF information:

    RefSeq Version XM_002689849.6
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689849.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCTGCCT TGAAGCGTCT CTGTCTAGGC AAAGAGCACA GCAGTCGGTC CTCCACCAGC
    AGCCTGGTCC CGGGCCCTGA GCCGGGCCCC CAACCTGCCC TGCACATCCA GGCGCAGGTG
    AACAACAGCA ACAACAAGAA AGGCACCTTC ACAGACGATC TGCACAAGCT GGTGGACGAG
    TGGACAAGCA AGACGGTCGG GGCCACACCA CTAAAGCCGT CACTCAACCA GTTGAAGCAG
    ACGCAGAAAT TGCATGACAT GGAGGCCACA GCAGGCTGGC CCCTGGCCTC TGGCGAGGCA
    CGGGCTATGA ACACATCTCG AACGGCAATG GGGATGCCAT GTCTGGCCCC GGTACCCAGC
    CCTGTACCCA CCGTGGTCAT TCCTGGAGCT GCCTCAGCCC TTCCTGTGCC CATGCCAGAT
    TCTGAAAGTG AGAAGCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237500
    Clone ID Related Accession (Same CDS sequence) XM_005210446.4
    Accession Version XM_005210446.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210446.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCCTCCA CCAGCAGCCT GGTCCCGGGC CCTGAGCCGG GCCCCCAACC TGCCCTGCAC
    ATCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAAGGCA CCTTCACAGA CGATCTGCAC
    AAGCTGGTGG ACGAGTGGAC AAGCAAGACG GTCGGGGCCA CACCACTAAA GCCGTCACTC
    AACCAGTTGA AGCAGACGCA GAAATTGCAT GACATGGAGG CCACAGCAGG CTGGCCCCTG
    GCCTCTGGCG AGGCACGGGC TATGAACACA TCTCGAACGG CAATGGGGAT GCCATGTCTG
    GCCCCGGTAC CCAGCCCTGT ACCCACCGTG GTCATTCCTG GAGCTGCCTC AGCCCTTCCT
    GTGCCCATGC CAGATTCTGA AAGTGAGAAG CCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210503.2
    CDS207..6665
    Translation

    Target ORF information:

    RefSeq Version XM_005210446.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210446.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCCTCCA CCAGCAGCCT GGTCCCGGGC CCTGAGCCGG GCCCCCAACC TGCCCTGCAC
    ATCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAAGGCA CCTTCACAGA CGATCTGCAC
    AAGCTGGTGG ACGAGTGGAC AAGCAAGACG GTCGGGGCCA CACCACTAAA GCCGTCACTC
    AACCAGTTGA AGCAGACGCA GAAATTGCAT GACATGGAGG CCACAGCAGG CTGGCCCCTG
    GCCTCTGGCG AGGCACGGGC TATGAACACA TCTCGAACGG CAATGGGGAT GCCATGTCTG
    GCCCCGGTAC CCAGCCCTGT ACCCACCGTG GTCATTCCTG GAGCTGCCTC AGCCCTTCCT
    GTGCCCATGC CAGATTCTGA AAGTGAGAAG CCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237501
    Clone ID Related Accession (Same CDS sequence) XM_005210447.4
    Accession Version XM_005210447.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210447.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCTGCC TTGAAGCGTC TCTGTCTAGG CAAAGAGCAC
    AGCAGTCGGT CCTCCACCAG CAGCCTGGTC CCGGGCCCTG AGCCGGGCCC CCAACCTGCC
    CTGCACATCC AGGCGCAGGT GAACAACAGC AACAACAAGA AAGGCACCTT CACAGACGAT
    CTGCACAAGC TGGTGGACGA GTGGACAAGC AAGACGGTCG GGGCCACACC ACTAAAGCCG
    TCACTCAACC AGTTGAAGCA GACGCAGAAA TTGCATGACA TGGAGGCCAC AGCAGGCTGG
    CCCCTGGCCT CTGGCGAGGC ACGGGCTATG AACACATCTC GAACGGCAAT GGGGATGCCA
    TGTCTGGCCC CGGTACCCAG CCCTGTACCC ACCGTGGTCA TTCCTGGAGC TGCCTCAGCC
    CTTCCTGTGC CCATGCCAGA TTCTGAAAGT GAGAAGCCCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210504.2
    CDS207..6611
    Translation

    Target ORF information:

    RefSeq Version XM_005210447.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210447.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCTGCC TTGAAGCGTC TCTGTCTAGG CAAAGAGCAC
    AGCAGTCGGT CCTCCACCAG CAGCCTGGTC CCGGGCCCTG AGCCGGGCCC CCAACCTGCC
    CTGCACATCC AGGCGCAGGT GAACAACAGC AACAACAAGA AAGGCACCTT CACAGACGAT
    CTGCACAAGC TGGTGGACGA GTGGACAAGC AAGACGGTCG GGGCCACACC ACTAAAGCCG
    TCACTCAACC AGTTGAAGCA GACGCAGAAA TTGCATGACA TGGAGGCCAC AGCAGGCTGG
    CCCCTGGCCT CTGGCGAGGC ACGGGCTATG AACACATCTC GAACGGCAAT GGGGATGCCA
    TGTCTGGCCC CGGTACCCAG CCCTGTACCC ACCGTGGTCA TTCCTGGAGC TGCCTCAGCC
    CTTCCTGTGC CCATGCCAGA TTCTGAAAGT GAGAAGCCCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237502
    Clone ID Related Accession (Same CDS sequence) XM_005210448.4
    Accession Version XM_005210448.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210448.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCCTCC ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG
    GGCCCCCAAC CTGCCCTGCA CATCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAAGGC
    ACCTTCACAG ACGATCTGCA CAAGCTGGTG GACGAGTGGA CAAGCAAGAC GGTCGGGGCC
    ACACCACTAA AGCCGTCACT CAACCAGTTG AAGCAGACGC AGAAATTGCA TGACATGGAG
    GCCACAGCAG GCTGGCCCCT GGCCTCTGGC GAGGCACGGG CTATGAACAC ATCTCGAACG
    GCAATGGGGA TGCCATGTCT GGCCCCGGTA CCCAGCCCTG TACCCACCGT GGTCATTCCT
    GGAGCTGCCT CAGCCCTTCC TGTGCCCATG CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210505.2
    CDS207..6566
    Translation

    Target ORF information:

    RefSeq Version XM_005210448.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210448.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCCTCC ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG
    GGCCCCCAAC CTGCCCTGCA CATCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAAGGC
    ACCTTCACAG ACGATCTGCA CAAGCTGGTG GACGAGTGGA CAAGCAAGAC GGTCGGGGCC
    ACACCACTAA AGCCGTCACT CAACCAGTTG AAGCAGACGC AGAAATTGCA TGACATGGAG
    GCCACAGCAG GCTGGCCCCT GGCCTCTGGC GAGGCACGGG CTATGAACAC ATCTCGAACG
    GCAATGGGGA TGCCATGTCT GGCCCCGGTA CCCAGCCCTG TACCCACCGT GGTCATTCCT
    GGAGCTGCCT CAGCCCTTCC TGTGCCCATG CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237503
    Clone ID Related Accession (Same CDS sequence) XM_005210449.4
    Accession Version XM_005210449.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210449.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCTGC CTTGAAGCGT CTCTGTCTAG GCAAAGAGCA CAGCAGTCGG
    TCCTCCACCA GCAGCCTGGT CCCGGGCCCT GAGCCGGGCC CCCAACCTGC CCTGCACATC
    CAGGCGCAGG TGAACAACAG CAACAACAAG AAAGGCACCT TCACAGACGA TCTGCACAAG
    CTGGTGGACG AGTGGACAAG CAAGACGGTC GGGGCCACAC CACTAAAGCC GTCACTCAAC
    CAGTTGAAGC AGACGCAGAA ATTGCATGAC ATGGAGGCCA CAGCAGGCTG GCCCCTGGCC
    TCTGGCGAGG CACGGGCTAT GAACACATCT CGAACGGCAA TGGGGATGCC ATGTCTGGCC
    CCGGTACCCA GCCCTGTACC CACCGTGGTC ATTCCTGGAG CTGCCTCAGC CCTTCCTGTG
    CCCATGCCAG ATTCTGAAAG TGAGAAGCCC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210506.2
    CDS207..6422
    Translation

    Target ORF information:

    RefSeq Version XM_005210449.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210449.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCTGC CTTGAAGCGT CTCTGTCTAG GCAAAGAGCA CAGCAGTCGG
    TCCTCCACCA GCAGCCTGGT CCCGGGCCCT GAGCCGGGCC CCCAACCTGC CCTGCACATC
    CAGGCGCAGG TGAACAACAG CAACAACAAG AAAGGCACCT TCACAGACGA TCTGCACAAG
    CTGGTGGACG AGTGGACAAG CAAGACGGTC GGGGCCACAC CACTAAAGCC GTCACTCAAC
    CAGTTGAAGC AGACGCAGAA ATTGCATGAC ATGGAGGCCA CAGCAGGCTG GCCCCTGGCC
    TCTGGCGAGG CACGGGCTAT GAACACATCT CGAACGGCAA TGGGGATGCC ATGTCTGGCC
    CCGGTACCCA GCCCTGTACC CACCGTGGTC ATTCCTGGAG CTGCCTCAGC CCTTCCTGTG
    CCCATGCCAG ATTCTGAAAG TGAGAAGCCC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237504
    Clone ID Related Accession (Same CDS sequence) XM_005210450.4
    Accession Version XM_005210450.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGGAGCAGGC CTCACAGGAC
    AAGCAGCCCA GCCTCCCTCA GAGCTGTGAG AGCTATGGAG GCTCTGACGT CCCTTCTGGA
    AGAGAGATGA GTGACAGCTG CGAAGGTGCA TTTGGTGGGG GAAAGCTGGA AGGCAAGGCC
    GCCCGGAAAC ATCACCGCAG GTCCACGCGT GTGCGCTCCC GCCAGGAGAG GGCCAGTCGG
    CCCCGACTGA CCATCCTGAA CGTGTGCAAC ACGGGTGACA AGATGGTGGA GTGCCAGTTG
    GAGACACACA ACCACAAGAT GGTGACGTTC AAGTTTGACC TGGATGGGGA TGCGCCTGAC
    GAGATCGCTA CCTACATGGT AGAGCACGAC TTCATCCTGC AGGCCGAGCG GGAGACATTC
    ATTGAGCAGA TGAAGGATGT CATGGACAAG GCAGAGGATA TGCTCAGTGA GGACACGGAT
    GTGGACCGTG CCTCCGACCC TGGGGTGAGC CCGCCACACC TCAGCACCTG TGGCCTGGGT
    GCTGGGGAGG AGAATCGCCA ATCCCAAGCA AACGCCCCGG TGTACCAGCA AAATGTCCTG
    CATACTGGGA AGAGGTGGTT TATCATCTGT CCGGTGGCCG AGCACCCAGC AGTAGAGGCC
    CCCGAATCTT CACCCCCACT TCCTCTGAGC TCCCTGCAGC CAGACACCAG CCAAGATTCA
    GCACCCTCTA AAGACCAGCT ATCCTCCAAG GAGAAACCCA GCTTCTCAAC TGATCTGCAG
    CTTCTGAGCC AAGCAGGCCC CAGGGACCTT CCTGGTGGTG CACCAGCCCC TTTGGTGCCA
    TCATCCCCTC CCGTGACTGC TCTGTCCCAA GACATGGCCC CACCAGCTGC CAGTCCTAAG
    CTGGAGCCAG CAACGGGGCC TGCCATGCAG GCAGGGGGAC CAGGAACCCC TCAGGGGCCA
    GCCAGTGAGC ATAAGACTCC CTGGCCATTA ATGGAAACTC TTGATGCTCA GCTTGCTGCA
    CAGTCCCCTG GCATGGGCCG AGGGCCTTGT GCCCCACCTT TGGAGGCCCC CCTTCACCCC
    ATGGGTCTGG GGGATCCTGC CTTAGCCAGA GAGCCCATCA CCAAGGGGGA GCCCCTGTCA
    GCTTTGGCCC CCACATCTGG TGCCCCTGGC GGGGCCTCTC AGCCCACTGT GAGCCAGCCC
    CTTCCCCCAC TCCCCACTGT GGTGGGAGCA ATCAGTCTGG CCACTCCCCA GCTCCCCAGT
    CCCCCGCTGG CACCCACTGC CCCACCTCAG CCGCCTTCAA CCTTGGAGTC AGATGGAGAA
    GGGCCGCCCC CAAGGGTGGG CTTTGTGGAC AGCACCATCA AGAGCCTGGA CGAGAAGCTG
    AGGACTCTGC TCTACCAGGA GCACGTGCCC ACCTCCTCAG CCTCCGCCGG GACCCCTGTA
    GAGGCAGGTG ACCGAGACTT CACCCTGGAA CCCCCAAGAG GGGATGAGCC CCGTACAGAG
    GCCTCCGGGG GCAGTCTGGG CCTGCTGCCC CAACCTGCGT TGGAAAAGTC AGAAGTGGCC
    CCTGGATTAG AGGTCACACT GGAGCAGTCC GTGTCCTTGT CAGTGACAGC TGAATCATCT
    TTGAGCCACA CACTTGGCCG TGAGGGAGCA CAAGTGGCCT CAGATTCGCG CACAGCACCC
    CGTGCCCCCC AGGATGTCCC TGCTTCTGCC ATCCCTGCCC ATCTGGAGCC CACAGACCGG
    ACCAGCAGGG TCCCTGGGGA AACCTTGGCT GAGTCCATGC AGAGCTGCCT GGGGATGTTG
    ATTGAGGGTG GCCAGGACAG CCAACCCCAG CCACACAGCA CTCTGGTATC AGGATCCCCT
    CTGAAGTGGC CAGGGCAGCA GGATGGCAGC TCGCCAGCCA AAACCGTGGG CCGGTTCTCA
    GTGGTCAGCA CCCAGGATGA GTGGACTCTG GCCTCCCCCA ATAGCCTGCG GTACTCAGCC
    CCACCTGACG TTTACCTGGA CGAGGCTCCC TGCAGCCCAG ACATGAAGCT GGCTGTGCGG
    CGGGCACAGA CGGCCTCCTC CATCGAGGTC GGCATGGAAG AGCCTTTGTC CAGTGACTCT
    GGGGATGAGT CCCCTCGCAG GAGGCCTTCT GTGCAGAAGC ATGCCTCCCT GCCCAGCAGC
    AGTGGTGGTG TGGCCAGTGA CTTTGTGAAG AAGGCCGCTG CCTTCCTGCA GAGGTCCTCT
    CGGGCTGGCT CCCCATGCCT GGAGACACCC AGCAGGATGG GTGTGAAGGT CCCCACCATC
    AGCGTGACCT CCTTCCACTC CCAGTCATCC TACATCAGCA GTGACAACGA CTCAGAGATT
    GAGGATGCTG ATATCAGGAA AGAGCTGCAG AGTCTTCGGG AGAAGCATCT GAAGGAGATC
    TCAGAGCTAC AAAGTCAGCA GAAACAGGAG ATTGAGGCCC TGTACCGCCG CCTAGGCAAG
    CCGCTACCCC CCAACTTAGG CCTCTTCCAC ACGGCCCCAC CCATTGGCCG CCGGAGAAAG
    ACCAGCAAGA GCAAGCTGAA GGCGGGGAAG TTGCTGAGCC CCCTGGTGCA GCAGCTCAAA
    GTCGTGGCCT CCAGCACAGG TCACTTGTCA GACTGCAGCA GAGCCCCTCC TGCTAAGGAC
    CCTGCCCAAG CTGGGGCAGG GCCCACAGCA GCTTCAGACC CATGTGGGAA GGCAGTTCAG
    ACTCAGCAGC CTTGCTCCGT GCGGGCCTCC CTGTCTGCAG ACATCTGCTC CGGCTTGGCC
    AGTGATGGAG GCGGAGCGCA CGGCCCAGGC TGGACGGTTT ACCACCCAAC GTCTGAGAGA
    GTGACCTATA AGTCTAGTAG CAAACCTCGC GCTCGATTCC TCAGTGGACC CGTGTCTGTG
    TCCATCTGGT CTGCCTTGAA GCGTCTCTGT CTAGGCAAAG AGCACAGCAG TCGGTCCTCC
    ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG GGCCCCCAAC CTGCCCTGCA CATCCAGGCG
    CAGGTGAACA ACAGCAACAA CAAGAAAGGC ACCTTCACAG ACGATCTGCA CAAGCTGGTG
    GACGAGTGGA CAAGCAAGAC GGTCGGGGCC ACACCACTAA AGCCGTCACT CAACCAGTTG
    AAGCAGACGC AGAAATTGCA TGACATGGAG GCCACAGCAG GCTGGCCCCT GGCCTCTGGC
    GAGGCACGGG CTATGAACAC ATCTCGAACG GCAATGGGGA TGCCATGTCT GGCCCCGGTA
    CCCAGCCCTG TACCCACCGT GGTCATTCCT GGAGCTGCCT CAGCCCTTCC TGTGCCCATG
    CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210507.2
    CDS207..6416
    Translation

    Target ORF information:

    RefSeq Version XM_005210450.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210450.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGGAGCAGGC CTCACAGGAC
    AAGCAGCCCA GCCTCCCTCA GAGCTGTGAG AGCTATGGAG GCTCTGACGT CCCTTCTGGA
    AGAGAGATGA GTGACAGCTG CGAAGGTGCA TTTGGTGGGG GAAAGCTGGA AGGCAAGGCC
    GCCCGGAAAC ATCACCGCAG GTCCACGCGT GTGCGCTCCC GCCAGGAGAG GGCCAGTCGG
    CCCCGACTGA CCATCCTGAA CGTGTGCAAC ACGGGTGACA AGATGGTGGA GTGCCAGTTG
    GAGACACACA ACCACAAGAT GGTGACGTTC AAGTTTGACC TGGATGGGGA TGCGCCTGAC
    GAGATCGCTA CCTACATGGT AGAGCACGAC TTCATCCTGC AGGCCGAGCG GGAGACATTC
    ATTGAGCAGA TGAAGGATGT CATGGACAAG GCAGAGGATA TGCTCAGTGA GGACACGGAT
    GTGGACCGTG CCTCCGACCC TGGGGTGAGC CCGCCACACC TCAGCACCTG TGGCCTGGGT
    GCTGGGGAGG AGAATCGCCA ATCCCAAGCA AACGCCCCGG TGTACCAGCA AAATGTCCTG
    CATACTGGGA AGAGGTGGTT TATCATCTGT CCGGTGGCCG AGCACCCAGC AGTAGAGGCC
    CCCGAATCTT CACCCCCACT TCCTCTGAGC TCCCTGCAGC CAGACACCAG CCAAGATTCA
    GCACCCTCTA AAGACCAGCT ATCCTCCAAG GAGAAACCCA GCTTCTCAAC TGATCTGCAG
    CTTCTGAGCC AAGCAGGCCC CAGGGACCTT CCTGGTGGTG CACCAGCCCC TTTGGTGCCA
    TCATCCCCTC CCGTGACTGC TCTGTCCCAA GACATGGCCC CACCAGCTGC CAGTCCTAAG
    CTGGAGCCAG CAACGGGGCC TGCCATGCAG GCAGGGGGAC CAGGAACCCC TCAGGGGCCA
    GCCAGTGAGC ATAAGACTCC CTGGCCATTA ATGGAAACTC TTGATGCTCA GCTTGCTGCA
    CAGTCCCCTG GCATGGGCCG AGGGCCTTGT GCCCCACCTT TGGAGGCCCC CCTTCACCCC
    ATGGGTCTGG GGGATCCTGC CTTAGCCAGA GAGCCCATCA CCAAGGGGGA GCCCCTGTCA
    GCTTTGGCCC CCACATCTGG TGCCCCTGGC GGGGCCTCTC AGCCCACTGT GAGCCAGCCC
    CTTCCCCCAC TCCCCACTGT GGTGGGAGCA ATCAGTCTGG CCACTCCCCA GCTCCCCAGT
    CCCCCGCTGG CACCCACTGC CCCACCTCAG CCGCCTTCAA CCTTGGAGTC AGATGGAGAA
    GGGCCGCCCC CAAGGGTGGG CTTTGTGGAC AGCACCATCA AGAGCCTGGA CGAGAAGCTG
    AGGACTCTGC TCTACCAGGA GCACGTGCCC ACCTCCTCAG CCTCCGCCGG GACCCCTGTA
    GAGGCAGGTG ACCGAGACTT CACCCTGGAA CCCCCAAGAG GGGATGAGCC CCGTACAGAG
    GCCTCCGGGG GCAGTCTGGG CCTGCTGCCC CAACCTGCGT TGGAAAAGTC AGAAGTGGCC
    CCTGGATTAG AGGTCACACT GGAGCAGTCC GTGTCCTTGT CAGTGACAGC TGAATCATCT
    TTGAGCCACA CACTTGGCCG TGAGGGAGCA CAAGTGGCCT CAGATTCGCG CACAGCACCC
    CGTGCCCCCC AGGATGTCCC TGCTTCTGCC ATCCCTGCCC ATCTGGAGCC CACAGACCGG
    ACCAGCAGGG TCCCTGGGGA AACCTTGGCT GAGTCCATGC AGAGCTGCCT GGGGATGTTG
    ATTGAGGGTG GCCAGGACAG CCAACCCCAG CCACACAGCA CTCTGGTATC AGGATCCCCT
    CTGAAGTGGC CAGGGCAGCA GGATGGCAGC TCGCCAGCCA AAACCGTGGG CCGGTTCTCA
    GTGGTCAGCA CCCAGGATGA GTGGACTCTG GCCTCCCCCA ATAGCCTGCG GTACTCAGCC
    CCACCTGACG TTTACCTGGA CGAGGCTCCC TGCAGCCCAG ACATGAAGCT GGCTGTGCGG
    CGGGCACAGA CGGCCTCCTC CATCGAGGTC GGCATGGAAG AGCCTTTGTC CAGTGACTCT
    GGGGATGAGT CCCCTCGCAG GAGGCCTTCT GTGCAGAAGC ATGCCTCCCT GCCCAGCAGC
    AGTGGTGGTG TGGCCAGTGA CTTTGTGAAG AAGGCCGCTG CCTTCCTGCA GAGGTCCTCT
    CGGGCTGGCT CCCCATGCCT GGAGACACCC AGCAGGATGG GTGTGAAGGT CCCCACCATC
    AGCGTGACCT CCTTCCACTC CCAGTCATCC TACATCAGCA GTGACAACGA CTCAGAGATT
    GAGGATGCTG ATATCAGGAA AGAGCTGCAG AGTCTTCGGG AGAAGCATCT GAAGGAGATC
    TCAGAGCTAC AAAGTCAGCA GAAACAGGAG ATTGAGGCCC TGTACCGCCG CCTAGGCAAG
    CCGCTACCCC CCAACTTAGG CCTCTTCCAC ACGGCCCCAC CCATTGGCCG CCGGAGAAAG
    ACCAGCAAGA GCAAGCTGAA GGCGGGGAAG TTGCTGAGCC CCCTGGTGCA GCAGCTCAAA
    GTCGTGGCCT CCAGCACAGG TCACTTGTCA GACTGCAGCA GAGCCCCTCC TGCTAAGGAC
    CCTGCCCAAG CTGGGGCAGG GCCCACAGCA GCTTCAGACC CATGTGGGAA GGCAGTTCAG
    ACTCAGCAGC CTTGCTCCGT GCGGGCCTCC CTGTCTGCAG ACATCTGCTC CGGCTTGGCC
    AGTGATGGAG GCGGAGCGCA CGGCCCAGGC TGGACGGTTT ACCACCCAAC GTCTGAGAGA
    GTGACCTATA AGTCTAGTAG CAAACCTCGC GCTCGATTCC TCAGTGGACC CGTGTCTGTG
    TCCATCTGGT CTGCCTTGAA GCGTCTCTGT CTAGGCAAAG AGCACAGCAG TCGGTCCTCC
    ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG GGCCCCCAAC CTGCCCTGCA CATCCAGGCG
    CAGGTGAACA ACAGCAACAA CAAGAAAGGC ACCTTCACAG ACGATCTGCA CAAGCTGGTG
    GACGAGTGGA CAAGCAAGAC GGTCGGGGCC ACACCACTAA AGCCGTCACT CAACCAGTTG
    AAGCAGACGC AGAAATTGCA TGACATGGAG GCCACAGCAG GCTGGCCCCT GGCCTCTGGC
    GAGGCACGGG CTATGAACAC ATCTCGAACG GCAATGGGGA TGCCATGTCT GGCCCCGGTA
    CCCAGCCCTG TACCCACCGT GGTCATTCCT GGAGCTGCCT CAGCCCTTCC TGTGCCCATG
    CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237505
    Clone ID Related Accession (Same CDS sequence) XM_005210451.4
    Accession Version XM_005210451.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210451.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCCTC CACCAGCAGC CTGGTCCCGG GCCCTGAGCC GGGCCCCCAA
    CCTGCCCTGC ACATCCAGGC GCAGGTGAAC AACAGCAACA ACAAGAAAGG CACCTTCACA
    GACGATCTGC ACAAGCTGGT GGACGAGTGG ACAAGCAAGA CGGTCGGGGC CACACCACTA
    AAGCCGTCAC TCAACCAGTT GAAGCAGACG CAGAAATTGC ATGACATGGA GGCCACAGCA
    GGCTGGCCCC TGGCCTCTGG CGAGGCACGG GCTATGAACA CATCTCGAAC GGCAATGGGG
    ATGCCATGTC TGGCCCCGGT ACCCAGCCCT GTACCCACCG TGGTCATTCC TGGAGCTGCC
    TCAGCCCTTC CTGTGCCCAT GCCAGATTCT GAAAGTGAGA AGCCCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210508.2
    CDS207..6377
    Translation

    Target ORF information:

    RefSeq Version XM_005210451.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210451.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGCG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCCTC CACCAGCAGC CTGGTCCCGG GCCCTGAGCC GGGCCCCCAA
    CCTGCCCTGC ACATCCAGGC GCAGGTGAAC AACAGCAACA ACAAGAAAGG CACCTTCACA
    GACGATCTGC ACAAGCTGGT GGACGAGTGG ACAAGCAAGA CGGTCGGGGC CACACCACTA
    AAGCCGTCAC TCAACCAGTT GAAGCAGACG CAGAAATTGC ATGACATGGA GGCCACAGCA
    GGCTGGCCCC TGGCCTCTGG CGAGGCACGG GCTATGAACA CATCTCGAAC GGCAATGGGG
    ATGCCATGTC TGGCCCCGGT ACCCAGCCCT GTACCCACCG TGGTCATTCC TGGAGCTGCC
    TCAGCCCTTC CTGTGCCCAT GCCAGATTCT GAAAGTGAGA AGCCCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22296
    Clone ID Related Accession (Same CDS sequence) XM_582977.9 , XM_002689849.5
    Accession Version XM_582977.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_582977.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCTGCCT TGAAGCGTCT CTGTCTAGGC AAAGAGCACA GCAGTCGGTC CTCCACCAGC
    AGCCTGGTCC CGGGCCCTGA GCCGGGCCCC CAACCTGCCC TGCACATCCA GGCGCAGGTG
    AACAACAGCA ACAACAAGAA AGGCACCTTC ACAGACGATC TGCACAAGCT GGTGGACGAG
    TGGACAAGCA AGACGGTCGG GGCCACACCA CTAAAGCCGT CACTCAACCA GTTGAAGCAG
    ACGCAGAAAT TGCATGACAT GGAGGCCACA GCAGGCTGGC CCCTGGCCTC TGGCGAGGCA
    CGGGCTATGA ACACATCTCG AACAGCAATG GGGATGCCAT GTCTGGCCCC GGTACCCAGC
    CCTGTACCCA CCGTGGTCAT TCCTGGAGCT GCCTCAGCCC TTCCTGTGCC CATGCCAGAT
    TCTGAAAGTG AGAAGCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_582977.7
    CDS211..6714
    Translation

    Target ORF information:

    RefSeq Version XM_582977.9
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_582977.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCTGCCT TGAAGCGTCT CTGTCTAGGC AAAGAGCACA GCAGTCGGTC CTCCACCAGC
    AGCCTGGTCC CGGGCCCTGA GCCGGGCCCC CAACCTGCCC TGCACATCCA GGCGCAGGTG
    AACAACAGCA ACAACAAGAA AGGCACCTTC ACAGACGATC TGCACAAGCT GGTGGACGAG
    TGGACAAGCA AGACGGTCGG GGCCACACCA CTAAAGCCGT CACTCAACCA GTTGAAGCAG
    ACGCAGAAAT TGCATGACAT GGAGGCCACA GCAGGCTGGC CCCTGGCCTC TGGCGAGGCA
    CGGGCTATGA ACACATCTCG AACAGCAATG GGGATGCCAT GTCTGGCCCC GGTACCCAGC
    CCTGTACCCA CCGTGGTCAT TCCTGGAGCT GCCTCAGCCC TTCCTGTGCC CATGCCAGAT
    TCTGAAAGTG AGAAGCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22296
    Clone ID Related Accession (Same CDS sequence) XM_582977.9 , XM_002689849.5
    Accession Version XM_002689849.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689849.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCTGCCT TGAAGCGTCT CTGTCTAGGC AAAGAGCACA GCAGTCGGTC CTCCACCAGC
    AGCCTGGTCC CGGGCCCTGA GCCGGGCCCC CAACCTGCCC TGCACATCCA GGCGCAGGTG
    AACAACAGCA ACAACAAGAA AGGCACCTTC ACAGACGATC TGCACAAGCT GGTGGACGAG
    TGGACAAGCA AGACGGTCGG GGCCACACCA CTAAAGCCGT CACTCAACCA GTTGAAGCAG
    ACGCAGAAAT TGCATGACAT GGAGGCCACA GCAGGCTGGC CCCTGGCCTC TGGCGAGGCA
    CGGGCTATGA ACACATCTCG AACAGCAATG GGGATGCCAT GTCTGGCCCC GGTACCCAGC
    CCTGTACCCA CCGTGGTCAT TCCTGGAGCT GCCTCAGCCC TTCCTGTGCC CATGCCAGAT
    TCTGAAAGTG AGAAGCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689895.3
    CDS211..6714
    Translation

    Target ORF information:

    RefSeq Version XM_002689849.5
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689849.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCTGCCT TGAAGCGTCT CTGTCTAGGC AAAGAGCACA GCAGTCGGTC CTCCACCAGC
    AGCCTGGTCC CGGGCCCTGA GCCGGGCCCC CAACCTGCCC TGCACATCCA GGCGCAGGTG
    AACAACAGCA ACAACAAGAA AGGCACCTTC ACAGACGATC TGCACAAGCT GGTGGACGAG
    TGGACAAGCA AGACGGTCGG GGCCACACCA CTAAAGCCGT CACTCAACCA GTTGAAGCAG
    ACGCAGAAAT TGCATGACAT GGAGGCCACA GCAGGCTGGC CCCTGGCCTC TGGCGAGGCA
    CGGGCTATGA ACACATCTCG AACAGCAATG GGGATGCCAT GTCTGGCCCC GGTACCCAGC
    CCTGTACCCA CCGTGGTCAT TCCTGGAGCT GCCTCAGCCC TTCCTGTGCC CATGCCAGAT
    TCTGAAAGTG AGAAGCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22297
    Clone ID Related Accession (Same CDS sequence) XM_005210446.3 , XM_005199273.3
    Accession Version XM_005210446.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCCTCCA CCAGCAGCCT GGTCCCGGGC CCTGAGCCGG GCCCCCAACC TGCCCTGCAC
    ATCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAAGGCA CCTTCACAGA CGATCTGCAC
    AAGCTGGTGG ACGAGTGGAC AAGCAAGACG GTCGGGGCCA CACCACTAAA GCCGTCACTC
    AACCAGTTGA AGCAGACGCA GAAATTGCAT GACATGGAGG CCACAGCAGG CTGGCCCCTG
    GCCTCTGGCG AGGCACGGGC TATGAACACA TCTCGAACAG CAATGGGGAT GCCATGTCTG
    GCCCCGGTAC CCAGCCCTGT ACCCACCGTG GTCATTCCTG GAGCTGCCTC AGCCCTTCCT
    GTGCCCATGC CAGATTCTGA AAGTGAGAAG CCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210503.1
    CDS211..6669
    Translation

    Target ORF information:

    RefSeq Version XM_005210446.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210446.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCCTCCA CCAGCAGCCT GGTCCCGGGC CCTGAGCCGG GCCCCCAACC TGCCCTGCAC
    ATCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAAGGCA CCTTCACAGA CGATCTGCAC
    AAGCTGGTGG ACGAGTGGAC AAGCAAGACG GTCGGGGCCA CACCACTAAA GCCGTCACTC
    AACCAGTTGA AGCAGACGCA GAAATTGCAT GACATGGAGG CCACAGCAGG CTGGCCCCTG
    GCCTCTGGCG AGGCACGGGC TATGAACACA TCTCGAACAG CAATGGGGAT GCCATGTCTG
    GCCCCGGTAC CCAGCCCTGT ACCCACCGTG GTCATTCCTG GAGCTGCCTC AGCCCTTCCT
    GTGCCCATGC CAGATTCTGA AAGTGAGAAG CCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22297
    Clone ID Related Accession (Same CDS sequence) XM_005210446.3 , XM_005199273.3
    Accession Version XM_005199273.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCCTCCA CCAGCAGCCT GGTCCCGGGC CCTGAGCCGG GCCCCCAACC TGCCCTGCAC
    ATCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAAGGCA CCTTCACAGA CGATCTGCAC
    AAGCTGGTGG ACGAGTGGAC AAGCAAGACG GTCGGGGCCA CACCACTAAA GCCGTCACTC
    AACCAGTTGA AGCAGACGCA GAAATTGCAT GACATGGAGG CCACAGCAGG CTGGCCCCTG
    GCCTCTGGCG AGGCACGGGC TATGAACACA TCTCGAACAG CAATGGGGAT GCCATGTCTG
    GCCCCGGTAC CCAGCCCTGT ACCCACCGTG GTCATTCCTG GAGCTGCCTC AGCCCTTCCT
    GTGCCCATGC CAGATTCTGA AAGTGAGAAG CCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199330.2
    CDS211..6669
    Translation

    Target ORF information:

    RefSeq Version XM_005199273.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199273.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGCTGGACG GTTTACCACC CAACGTCTGA GAGAGTGACC
    TATAAGTCTA GTAGCAAACC TCGCGCTCGA TTCCTCAGTG GACCCGTGTC TGTGTCCATC
    TGGTCCTCCA CCAGCAGCCT GGTCCCGGGC CCTGAGCCGG GCCCCCAACC TGCCCTGCAC
    ATCCAGGCGC AGGTGAACAA CAGCAACAAC AAGAAAGGCA CCTTCACAGA CGATCTGCAC
    AAGCTGGTGG ACGAGTGGAC AAGCAAGACG GTCGGGGCCA CACCACTAAA GCCGTCACTC
    AACCAGTTGA AGCAGACGCA GAAATTGCAT GACATGGAGG CCACAGCAGG CTGGCCCCTG
    GCCTCTGGCG AGGCACGGGC TATGAACACA TCTCGAACAG CAATGGGGAT GCCATGTCTG
    GCCCCGGTAC CCAGCCCTGT ACCCACCGTG GTCATTCCTG GAGCTGCCTC AGCCCTTCCT
    GTGCCCATGC CAGATTCTGA AAGTGAGAAG CCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22298
    Clone ID Related Accession (Same CDS sequence) XM_005199274.3 , XM_005210447.3
    Accession Version XM_005199274.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199274.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCTGCC TTGAAGCGTC TCTGTCTAGG CAAAGAGCAC
    AGCAGTCGGT CCTCCACCAG CAGCCTGGTC CCGGGCCCTG AGCCGGGCCC CCAACCTGCC
    CTGCACATCC AGGCGCAGGT GAACAACAGC AACAACAAGA AAGGCACCTT CACAGACGAT
    CTGCACAAGC TGGTGGACGA GTGGACAAGC AAGACGGTCG GGGCCACACC ACTAAAGCCG
    TCACTCAACC AGTTGAAGCA GACGCAGAAA TTGCATGACA TGGAGGCCAC AGCAGGCTGG
    CCCCTGGCCT CTGGCGAGGC ACGGGCTATG AACACATCTC GAACAGCAAT GGGGATGCCA
    TGTCTGGCCC CGGTACCCAG CCCTGTACCC ACCGTGGTCA TTCCTGGAGC TGCCTCAGCC
    CTTCCTGTGC CCATGCCAGA TTCTGAAAGT GAGAAGCCCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199331.2
    CDS211..6615
    Translation

    Target ORF information:

    RefSeq Version XM_005199274.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199274.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCTGCC TTGAAGCGTC TCTGTCTAGG CAAAGAGCAC
    AGCAGTCGGT CCTCCACCAG CAGCCTGGTC CCGGGCCCTG AGCCGGGCCC CCAACCTGCC
    CTGCACATCC AGGCGCAGGT GAACAACAGC AACAACAAGA AAGGCACCTT CACAGACGAT
    CTGCACAAGC TGGTGGACGA GTGGACAAGC AAGACGGTCG GGGCCACACC ACTAAAGCCG
    TCACTCAACC AGTTGAAGCA GACGCAGAAA TTGCATGACA TGGAGGCCAC AGCAGGCTGG
    CCCCTGGCCT CTGGCGAGGC ACGGGCTATG AACACATCTC GAACAGCAAT GGGGATGCCA
    TGTCTGGCCC CGGTACCCAG CCCTGTACCC ACCGTGGTCA TTCCTGGAGC TGCCTCAGCC
    CTTCCTGTGC CCATGCCAGA TTCTGAAAGT GAGAAGCCCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22298
    Clone ID Related Accession (Same CDS sequence) XM_005199274.3 , XM_005210447.3
    Accession Version XM_005210447.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCTGCC TTGAAGCGTC TCTGTCTAGG CAAAGAGCAC
    AGCAGTCGGT CCTCCACCAG CAGCCTGGTC CCGGGCCCTG AGCCGGGCCC CCAACCTGCC
    CTGCACATCC AGGCGCAGGT GAACAACAGC AACAACAAGA AAGGCACCTT CACAGACGAT
    CTGCACAAGC TGGTGGACGA GTGGACAAGC AAGACGGTCG GGGCCACACC ACTAAAGCCG
    TCACTCAACC AGTTGAAGCA GACGCAGAAA TTGCATGACA TGGAGGCCAC AGCAGGCTGG
    CCCCTGGCCT CTGGCGAGGC ACGGGCTATG AACACATCTC GAACAGCAAT GGGGATGCCA
    TGTCTGGCCC CGGTACCCAG CCCTGTACCC ACCGTGGTCA TTCCTGGAGC TGCCTCAGCC
    CTTCCTGTGC CCATGCCAGA TTCTGAAAGT GAGAAGCCCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210504.1
    CDS211..6615
    Translation

    Target ORF information:

    RefSeq Version XM_005210447.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210447.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCTGCC TTGAAGCGTC TCTGTCTAGG CAAAGAGCAC
    AGCAGTCGGT CCTCCACCAG CAGCCTGGTC CCGGGCCCTG AGCCGGGCCC CCAACCTGCC
    CTGCACATCC AGGCGCAGGT GAACAACAGC AACAACAAGA AAGGCACCTT CACAGACGAT
    CTGCACAAGC TGGTGGACGA GTGGACAAGC AAGACGGTCG GGGCCACACC ACTAAAGCCG
    TCACTCAACC AGTTGAAGCA GACGCAGAAA TTGCATGACA TGGAGGCCAC AGCAGGCTGG
    CCCCTGGCCT CTGGCGAGGC ACGGGCTATG AACACATCTC GAACAGCAAT GGGGATGCCA
    TGTCTGGCCC CGGTACCCAG CCCTGTACCC ACCGTGGTCA TTCCTGGAGC TGCCTCAGCC
    CTTCCTGTGC CCATGCCAGA TTCTGAAAGT GAGAAGCCCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22299
    Clone ID Related Accession (Same CDS sequence) XM_005199275.3 , XM_005210448.3
    Accession Version XM_005199275.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199275.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCCTCC ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG
    GGCCCCCAAC CTGCCCTGCA CATCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAAGGC
    ACCTTCACAG ACGATCTGCA CAAGCTGGTG GACGAGTGGA CAAGCAAGAC GGTCGGGGCC
    ACACCACTAA AGCCGTCACT CAACCAGTTG AAGCAGACGC AGAAATTGCA TGACATGGAG
    GCCACAGCAG GCTGGCCCCT GGCCTCTGGC GAGGCACGGG CTATGAACAC ATCTCGAACA
    GCAATGGGGA TGCCATGTCT GGCCCCGGTA CCCAGCCCTG TACCCACCGT GGTCATTCCT
    GGAGCTGCCT CAGCCCTTCC TGTGCCCATG CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199332.2
    CDS211..6570
    Translation

    Target ORF information:

    RefSeq Version XM_005199275.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199275.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCCTCC ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG
    GGCCCCCAAC CTGCCCTGCA CATCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAAGGC
    ACCTTCACAG ACGATCTGCA CAAGCTGGTG GACGAGTGGA CAAGCAAGAC GGTCGGGGCC
    ACACCACTAA AGCCGTCACT CAACCAGTTG AAGCAGACGC AGAAATTGCA TGACATGGAG
    GCCACAGCAG GCTGGCCCCT GGCCTCTGGC GAGGCACGGG CTATGAACAC ATCTCGAACA
    GCAATGGGGA TGCCATGTCT GGCCCCGGTA CCCAGCCCTG TACCCACCGT GGTCATTCCT
    GGAGCTGCCT CAGCCCTTCC TGTGCCCATG CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22299
    Clone ID Related Accession (Same CDS sequence) XM_005199275.3 , XM_005210448.3
    Accession Version XM_005210448.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCCTCC ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG
    GGCCCCCAAC CTGCCCTGCA CATCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAAGGC
    ACCTTCACAG ACGATCTGCA CAAGCTGGTG GACGAGTGGA CAAGCAAGAC GGTCGGGGCC
    ACACCACTAA AGCCGTCACT CAACCAGTTG AAGCAGACGC AGAAATTGCA TGACATGGAG
    GCCACAGCAG GCTGGCCCCT GGCCTCTGGC GAGGCACGGG CTATGAACAC ATCTCGAACA
    GCAATGGGGA TGCCATGTCT GGCCCCGGTA CCCAGCCCTG TACCCACCGT GGTCATTCCT
    GGAGCTGCCT CAGCCCTTCC TGTGCCCATG CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210505.1
    CDS211..6570
    Translation

    Target ORF information:

    RefSeq Version XM_005210448.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210448.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGTCACTT GTCAGACTGC AGCAGAGCCC CTCCTGCTAA GGACCCTGCC
    CAAGCTGGGG CAGGGCCCAC AGCAGCTTCA GACCCATGTG GGAAGGCAGT TCAGACTCAG
    CAGCCTTGCT CCGTGCGGGC CTCCCTGTCT GCAGACATCT GCTCCGGCTT GGCCAGTGAT
    GGAGGCGGAG CGCACGGCCC AGGGTCCTCC ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG
    GGCCCCCAAC CTGCCCTGCA CATCCAGGCG CAGGTGAACA ACAGCAACAA CAAGAAAGGC
    ACCTTCACAG ACGATCTGCA CAAGCTGGTG GACGAGTGGA CAAGCAAGAC GGTCGGGGCC
    ACACCACTAA AGCCGTCACT CAACCAGTTG AAGCAGACGC AGAAATTGCA TGACATGGAG
    GCCACAGCAG GCTGGCCCCT GGCCTCTGGC GAGGCACGGG CTATGAACAC ATCTCGAACA
    GCAATGGGGA TGCCATGTCT GGCCCCGGTA CCCAGCCCTG TACCCACCGT GGTCATTCCT
    GGAGCTGCCT CAGCCCTTCC TGTGCCCATG CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22300
    Clone ID Related Accession (Same CDS sequence) XM_005210449.3 , XM_005199276.3
    Accession Version XM_005210449.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCTGC CTTGAAGCGT CTCTGTCTAG GCAAAGAGCA CAGCAGTCGG
    TCCTCCACCA GCAGCCTGGT CCCGGGCCCT GAGCCGGGCC CCCAACCTGC CCTGCACATC
    CAGGCGCAGG TGAACAACAG CAACAACAAG AAAGGCACCT TCACAGACGA TCTGCACAAG
    CTGGTGGACG AGTGGACAAG CAAGACGGTC GGGGCCACAC CACTAAAGCC GTCACTCAAC
    CAGTTGAAGC AGACGCAGAA ATTGCATGAC ATGGAGGCCA CAGCAGGCTG GCCCCTGGCC
    TCTGGCGAGG CACGGGCTAT GAACACATCT CGAACAGCAA TGGGGATGCC ATGTCTGGCC
    CCGGTACCCA GCCCTGTACC CACCGTGGTC ATTCCTGGAG CTGCCTCAGC CCTTCCTGTG
    CCCATGCCAG ATTCTGAAAG TGAGAAGCCC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210506.1
    CDS211..6426
    Translation

    Target ORF information:

    RefSeq Version XM_005210449.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210449.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCTGC CTTGAAGCGT CTCTGTCTAG GCAAAGAGCA CAGCAGTCGG
    TCCTCCACCA GCAGCCTGGT CCCGGGCCCT GAGCCGGGCC CCCAACCTGC CCTGCACATC
    CAGGCGCAGG TGAACAACAG CAACAACAAG AAAGGCACCT TCACAGACGA TCTGCACAAG
    CTGGTGGACG AGTGGACAAG CAAGACGGTC GGGGCCACAC CACTAAAGCC GTCACTCAAC
    CAGTTGAAGC AGACGCAGAA ATTGCATGAC ATGGAGGCCA CAGCAGGCTG GCCCCTGGCC
    TCTGGCGAGG CACGGGCTAT GAACACATCT CGAACAGCAA TGGGGATGCC ATGTCTGGCC
    CCGGTACCCA GCCCTGTACC CACCGTGGTC ATTCCTGGAG CTGCCTCAGC CCTTCCTGTG
    CCCATGCCAG ATTCTGAAAG TGAGAAGCCC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22300
    Clone ID Related Accession (Same CDS sequence) XM_005210449.3 , XM_005199276.3
    Accession Version XM_005199276.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199276.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCTGC CTTGAAGCGT CTCTGTCTAG GCAAAGAGCA CAGCAGTCGG
    TCCTCCACCA GCAGCCTGGT CCCGGGCCCT GAGCCGGGCC CCCAACCTGC CCTGCACATC
    CAGGCGCAGG TGAACAACAG CAACAACAAG AAAGGCACCT TCACAGACGA TCTGCACAAG
    CTGGTGGACG AGTGGACAAG CAAGACGGTC GGGGCCACAC CACTAAAGCC GTCACTCAAC
    CAGTTGAAGC AGACGCAGAA ATTGCATGAC ATGGAGGCCA CAGCAGGCTG GCCCCTGGCC
    TCTGGCGAGG CACGGGCTAT GAACACATCT CGAACAGCAA TGGGGATGCC ATGTCTGGCC
    CCGGTACCCA GCCCTGTACC CACCGTGGTC ATTCCTGGAG CTGCCTCAGC CCTTCCTGTG
    CCCATGCCAG ATTCTGAAAG TGAGAAGCCC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199333.2
    CDS211..6426
    Translation

    Target ORF information:

    RefSeq Version XM_005199276.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199276.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCTGC CTTGAAGCGT CTCTGTCTAG GCAAAGAGCA CAGCAGTCGG
    TCCTCCACCA GCAGCCTGGT CCCGGGCCCT GAGCCGGGCC CCCAACCTGC CCTGCACATC
    CAGGCGCAGG TGAACAACAG CAACAACAAG AAAGGCACCT TCACAGACGA TCTGCACAAG
    CTGGTGGACG AGTGGACAAG CAAGACGGTC GGGGCCACAC CACTAAAGCC GTCACTCAAC
    CAGTTGAAGC AGACGCAGAA ATTGCATGAC ATGGAGGCCA CAGCAGGCTG GCCCCTGGCC
    TCTGGCGAGG CACGGGCTAT GAACACATCT CGAACAGCAA TGGGGATGCC ATGTCTGGCC
    CCGGTACCCA GCCCTGTACC CACCGTGGTC ATTCCTGGAG CTGCCTCAGC CCTTCCTGTG
    CCCATGCCAG ATTCTGAAAG TGAGAAGCCC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22301
    Clone ID Related Accession (Same CDS sequence) XM_005210450.3 , XM_005199277.3
    Accession Version XM_005210450.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGGAGCAGGC CTCACAGGAC
    AAGCAGCCCA GCCTCCCTCA GAGCTGTGAG AGCTATGGAG GCTCTGACGT CCCTTCTGGA
    AGAGAGATGA GTGACAGCTG CGAAGGTGCA TTTGGTGGGG GAAAGCTGGA AGGCAAGGCC
    GCCCGGAAAC ATCACCGCAG GTCCACGCGT GTGCGCTCCC GCCAGGAGAG GGCCAGTCGG
    CCCCGACTGA CCATCCTGAA CGTGTGCAAC ACGGGTGACA AGATGGTGGA GTGCCAGTTG
    GAGACACACA ACCACAAGAT GGTGACGTTC AAGTTTGACC TGGATGGGGA TGCGCCTGAC
    GAGATCGCTA CCTACATGGT AGAGCACGAC TTCATCCTGC AGGCCGAGCG GGAGACATTC
    ATTGAGCAGA TGAAGGATGT CATGGACAAG GCAGAGGATA TGCTCAGTGA GGACACGGAT
    GTGGACCGTG CCTCCGACCC TGGGGTGAGC CCGCCACACC TCAGCACCTG TGGCCTGGGT
    GCTGGGGAGG AGAATCGCCA ATCCCAAGCA AACGCCCCGG TGTACCAGCA AAATGTCCTG
    CATACTGGGA AGAGGTGGTT TATCATCTGT CCGGTGGCCG AGCACCCAGC AGTAGAGGCC
    CCCGAATCTT CACCCCCACT TCCTCTGAGC TCCCTGCAGC CAGACACCAG CCAAGATTCA
    GCACCCTCTA AAGACCAGCT ATCCTCCAAG GAGAAACCCA GCTTCTCAAC TGATCTGCAG
    CTTCTGAGCC AAGCAGGCCC CAGGGACCTT CCTGGTGGTG CACCAGCCCC TTTGGTGCCA
    TCATCCCCTC CCGTGACTGC TCTGTCCCAA GACATGGCCC CACCAGCTGC CAGTCCTAAG
    CTGGAGCCAG CAACGGGGCC TGCCATGCAG GCAGGGGGAC CAGGAACCCC TCAGGGGCCA
    GCCAGTGAGC ATAAGACTCC CTGGCCATTA ATGGAAACTC TTGATGCTCA GCTTGCTGCA
    CAGTCCCCTG GCATGGGCCG AGGGCCTTGT GCCCCACCTT TGGAGGCCCC CCTTCACCCC
    ATGGGTCTGG GGGATCCTGC CTTAGCCAGA GAGCCCATCA CCAAGGGGGA GCCCCTGTCA
    GCTTTGGCCC CCACATCTGG TGCCCCTGGC GGGGCCTCTC AGCCCACTGT GAGCCAGCCC
    CTTCCCCCAC TCCCCACTGT GGTGGGAGCA ATCAGTCTGG CCACTCCCCA GCTCCCCAGT
    CCCCCGCTGG CACCCACTGC CCCACCTCAG CCGCCTTCAA CCTTGGAGTC AGATGGAGAA
    GGGCCGCCCC CAAGGGTGGG CTTTGTGGAC AGCACCATCA AGAGCCTGGA CGAGAAGCTG
    AGGACTCTGC TCTACCAGGA GCACGTGCCC ACCTCCTCAG CCTCCGCCGG GACCCCTGTA
    GAGGCAGGTG ACCGAGACTT CACCCTGGAA CCCCCAAGAG GGGATGAGCC CCGTACAGAG
    GCCTCCGGGG GCAGTCTGGG CCTGCTGCCC CAACCTGCGT TGGAAAAGTC AGAAGTGGCC
    CCTGGATTAG AGGTCACACT GGAGCAGTCC GTGTCCTTGT CAGTGACAGC TGAATCATCT
    TTGAGCCACA CACTTGGCCG TGAGGGAGCA CAAGTGGCCT CAGATTCGCG CACAGCACCC
    CGTGCCCCCC AGGATGTCCC TGCTTCTGCC ATCCCTGCCC ATCTGGAGCC CACAGACCGG
    ACCAGCAGGG TCCCTGGGGA AACCTTGGCT GAGTCCATGC AGAGCTGCCT GGGGATGTTG
    ATTGAGGGTG GCCAGGACAG CCAACCCCAG CCACACAGCA CTCTGGTATC AGGATCCCCT
    CTGAAGTGGC CAGGGCAGCA GGATGGCAGC TCGCCAGCCA AAACCGTGGG CCGGTTCTCA
    GTGGTCAGCA CCCAGGATGA GTGGACTCTG GCCTCCCCCA ATAGCCTGCG GTACTCAGCC
    CCACCTGACG TTTACCTGGA CGAGGCTCCC TGCAGCCCAG ACATGAAGCT GGCTGTGCGG
    CGGGCACAGA CGGCCTCCTC CATCGAGGTC GGCATGGAAG AGCCTTTGTC CAGTGACTCT
    GGGGATGAGT CCCCTCGCAG GAGGCCTTCT GTGCAGAAGC ATGCCTCCCT GCCCAGCAGC
    AGTGGTGGTG TGGCCAGTGA CTTTGTGAAG AAGGCCGCTG CCTTCCTGCA GAGGTCCTCT
    CGGGCTGGCT CCCCATGCCT GGAGACACCC AGCAGGATGG GTGTGAAGGT CCCCACCATC
    AGCGTGACCT CCTTCCACTC CCAGTCATCC TACATCAGCA GTGACAACGA CTCAGAGATT
    GAGGATGCTG ATATCAGGAA AGAGCTGCAG AGTCTTCGGG AGAAGCATCT GAAGGAGATC
    TCAGAGCTAC AAAGTCAGCA GAAACAGGAG ATTGAGGCCC TGTACCGCCG CCTAGGCAAG
    CCGCTACCCC CCAACTTAGG CCTCTTCCAC ACGGCCCCAC CCATTGGCCG CCGGAGAAAG
    ACCAGCAAGA GCAAGCTGAA GGCGGGGAAG TTGCTGAGCC CCCTGGTGCA GCAGCTCAAA
    GTCGTGGCCT CCAGCACAGG TCACTTGTCA GACTGCAGCA GAGCCCCTCC TGCTAAGGAC
    CCTGCCCAAG CTGGGGCAGG GCCCACAGCA GCTTCAGACC CATGTGGGAA GGCAGTTCAG
    ACTCAGCAGC CTTGCTCCGT GCGGGCCTCC CTGTCTGCAG ACATCTGCTC CGGCTTGGCC
    AGTGATGGAG GCGGAGCGCA CGGCCCAGGC TGGACGGTTT ACCACCCAAC GTCTGAGAGA
    GTGACCTATA AGTCTAGTAG CAAACCTCGC GCTCGATTCC TCAGTGGACC CGTGTCTGTG
    TCCATCTGGT CTGCCTTGAA GCGTCTCTGT CTAGGCAAAG AGCACAGCAG TCGGTCCTCC
    ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG GGCCCCCAAC CTGCCCTGCA CATCCAGGCG
    CAGGTGAACA ACAGCAACAA CAAGAAAGGC ACCTTCACAG ACGATCTGCA CAAGCTGGTG
    GACGAGTGGA CAAGCAAGAC GGTCGGGGCC ACACCACTAA AGCCGTCACT CAACCAGTTG
    AAGCAGACGC AGAAATTGCA TGACATGGAG GCCACAGCAG GCTGGCCCCT GGCCTCTGGC
    GAGGCACGGG CTATGAACAC ATCTCGAACA GCAATGGGGA TGCCATGTCT GGCCCCGGTA
    CCCAGCCCTG TACCCACCGT GGTCATTCCT GGAGCTGCCT CAGCCCTTCC TGTGCCCATG
    CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210507.1
    CDS210..6419
    Translation

    Target ORF information:

    RefSeq Version XM_005210450.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210450.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGGAGCAGGC CTCACAGGAC
    AAGCAGCCCA GCCTCCCTCA GAGCTGTGAG AGCTATGGAG GCTCTGACGT CCCTTCTGGA
    AGAGAGATGA GTGACAGCTG CGAAGGTGCA TTTGGTGGGG GAAAGCTGGA AGGCAAGGCC
    GCCCGGAAAC ATCACCGCAG GTCCACGCGT GTGCGCTCCC GCCAGGAGAG GGCCAGTCGG
    CCCCGACTGA CCATCCTGAA CGTGTGCAAC ACGGGTGACA AGATGGTGGA GTGCCAGTTG
    GAGACACACA ACCACAAGAT GGTGACGTTC AAGTTTGACC TGGATGGGGA TGCGCCTGAC
    GAGATCGCTA CCTACATGGT AGAGCACGAC TTCATCCTGC AGGCCGAGCG GGAGACATTC
    ATTGAGCAGA TGAAGGATGT CATGGACAAG GCAGAGGATA TGCTCAGTGA GGACACGGAT
    GTGGACCGTG CCTCCGACCC TGGGGTGAGC CCGCCACACC TCAGCACCTG TGGCCTGGGT
    GCTGGGGAGG AGAATCGCCA ATCCCAAGCA AACGCCCCGG TGTACCAGCA AAATGTCCTG
    CATACTGGGA AGAGGTGGTT TATCATCTGT CCGGTGGCCG AGCACCCAGC AGTAGAGGCC
    CCCGAATCTT CACCCCCACT TCCTCTGAGC TCCCTGCAGC CAGACACCAG CCAAGATTCA
    GCACCCTCTA AAGACCAGCT ATCCTCCAAG GAGAAACCCA GCTTCTCAAC TGATCTGCAG
    CTTCTGAGCC AAGCAGGCCC CAGGGACCTT CCTGGTGGTG CACCAGCCCC TTTGGTGCCA
    TCATCCCCTC CCGTGACTGC TCTGTCCCAA GACATGGCCC CACCAGCTGC CAGTCCTAAG
    CTGGAGCCAG CAACGGGGCC TGCCATGCAG GCAGGGGGAC CAGGAACCCC TCAGGGGCCA
    GCCAGTGAGC ATAAGACTCC CTGGCCATTA ATGGAAACTC TTGATGCTCA GCTTGCTGCA
    CAGTCCCCTG GCATGGGCCG AGGGCCTTGT GCCCCACCTT TGGAGGCCCC CCTTCACCCC
    ATGGGTCTGG GGGATCCTGC CTTAGCCAGA GAGCCCATCA CCAAGGGGGA GCCCCTGTCA
    GCTTTGGCCC CCACATCTGG TGCCCCTGGC GGGGCCTCTC AGCCCACTGT GAGCCAGCCC
    CTTCCCCCAC TCCCCACTGT GGTGGGAGCA ATCAGTCTGG CCACTCCCCA GCTCCCCAGT
    CCCCCGCTGG CACCCACTGC CCCACCTCAG CCGCCTTCAA CCTTGGAGTC AGATGGAGAA
    GGGCCGCCCC CAAGGGTGGG CTTTGTGGAC AGCACCATCA AGAGCCTGGA CGAGAAGCTG
    AGGACTCTGC TCTACCAGGA GCACGTGCCC ACCTCCTCAG CCTCCGCCGG GACCCCTGTA
    GAGGCAGGTG ACCGAGACTT CACCCTGGAA CCCCCAAGAG GGGATGAGCC CCGTACAGAG
    GCCTCCGGGG GCAGTCTGGG CCTGCTGCCC CAACCTGCGT TGGAAAAGTC AGAAGTGGCC
    CCTGGATTAG AGGTCACACT GGAGCAGTCC GTGTCCTTGT CAGTGACAGC TGAATCATCT
    TTGAGCCACA CACTTGGCCG TGAGGGAGCA CAAGTGGCCT CAGATTCGCG CACAGCACCC
    CGTGCCCCCC AGGATGTCCC TGCTTCTGCC ATCCCTGCCC ATCTGGAGCC CACAGACCGG
    ACCAGCAGGG TCCCTGGGGA AACCTTGGCT GAGTCCATGC AGAGCTGCCT GGGGATGTTG
    ATTGAGGGTG GCCAGGACAG CCAACCCCAG CCACACAGCA CTCTGGTATC AGGATCCCCT
    CTGAAGTGGC CAGGGCAGCA GGATGGCAGC TCGCCAGCCA AAACCGTGGG CCGGTTCTCA
    GTGGTCAGCA CCCAGGATGA GTGGACTCTG GCCTCCCCCA ATAGCCTGCG GTACTCAGCC
    CCACCTGACG TTTACCTGGA CGAGGCTCCC TGCAGCCCAG ACATGAAGCT GGCTGTGCGG
    CGGGCACAGA CGGCCTCCTC CATCGAGGTC GGCATGGAAG AGCCTTTGTC CAGTGACTCT
    GGGGATGAGT CCCCTCGCAG GAGGCCTTCT GTGCAGAAGC ATGCCTCCCT GCCCAGCAGC
    AGTGGTGGTG TGGCCAGTGA CTTTGTGAAG AAGGCCGCTG CCTTCCTGCA GAGGTCCTCT
    CGGGCTGGCT CCCCATGCCT GGAGACACCC AGCAGGATGG GTGTGAAGGT CCCCACCATC
    AGCGTGACCT CCTTCCACTC CCAGTCATCC TACATCAGCA GTGACAACGA CTCAGAGATT
    GAGGATGCTG ATATCAGGAA AGAGCTGCAG AGTCTTCGGG AGAAGCATCT GAAGGAGATC
    TCAGAGCTAC AAAGTCAGCA GAAACAGGAG ATTGAGGCCC TGTACCGCCG CCTAGGCAAG
    CCGCTACCCC CCAACTTAGG CCTCTTCCAC ACGGCCCCAC CCATTGGCCG CCGGAGAAAG
    ACCAGCAAGA GCAAGCTGAA GGCGGGGAAG TTGCTGAGCC CCCTGGTGCA GCAGCTCAAA
    GTCGTGGCCT CCAGCACAGG TCACTTGTCA GACTGCAGCA GAGCCCCTCC TGCTAAGGAC
    CCTGCCCAAG CTGGGGCAGG GCCCACAGCA GCTTCAGACC CATGTGGGAA GGCAGTTCAG
    ACTCAGCAGC CTTGCTCCGT GCGGGCCTCC CTGTCTGCAG ACATCTGCTC CGGCTTGGCC
    AGTGATGGAG GCGGAGCGCA CGGCCCAGGC TGGACGGTTT ACCACCCAAC GTCTGAGAGA
    GTGACCTATA AGTCTAGTAG CAAACCTCGC GCTCGATTCC TCAGTGGACC CGTGTCTGTG
    TCCATCTGGT CTGCCTTGAA GCGTCTCTGT CTAGGCAAAG AGCACAGCAG TCGGTCCTCC
    ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG GGCCCCCAAC CTGCCCTGCA CATCCAGGCG
    CAGGTGAACA ACAGCAACAA CAAGAAAGGC ACCTTCACAG ACGATCTGCA CAAGCTGGTG
    GACGAGTGGA CAAGCAAGAC GGTCGGGGCC ACACCACTAA AGCCGTCACT CAACCAGTTG
    AAGCAGACGC AGAAATTGCA TGACATGGAG GCCACAGCAG GCTGGCCCCT GGCCTCTGGC
    GAGGCACGGG CTATGAACAC ATCTCGAACA GCAATGGGGA TGCCATGTCT GGCCCCGGTA
    CCCAGCCCTG TACCCACCGT GGTCATTCCT GGAGCTGCCT CAGCCCTTCC TGTGCCCATG
    CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22301
    Clone ID Related Accession (Same CDS sequence) XM_005210450.3 , XM_005199277.3
    Accession Version XM_005199277.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199277.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGGAGCAGGC CTCACAGGAC
    AAGCAGCCCA GCCTCCCTCA GAGCTGTGAG AGCTATGGAG GCTCTGACGT CCCTTCTGGA
    AGAGAGATGA GTGACAGCTG CGAAGGTGCA TTTGGTGGGG GAAAGCTGGA AGGCAAGGCC
    GCCCGGAAAC ATCACCGCAG GTCCACGCGT GTGCGCTCCC GCCAGGAGAG GGCCAGTCGG
    CCCCGACTGA CCATCCTGAA CGTGTGCAAC ACGGGTGACA AGATGGTGGA GTGCCAGTTG
    GAGACACACA ACCACAAGAT GGTGACGTTC AAGTTTGACC TGGATGGGGA TGCGCCTGAC
    GAGATCGCTA CCTACATGGT AGAGCACGAC TTCATCCTGC AGGCCGAGCG GGAGACATTC
    ATTGAGCAGA TGAAGGATGT CATGGACAAG GCAGAGGATA TGCTCAGTGA GGACACGGAT
    GTGGACCGTG CCTCCGACCC TGGGGTGAGC CCGCCACACC TCAGCACCTG TGGCCTGGGT
    GCTGGGGAGG AGAATCGCCA ATCCCAAGCA AACGCCCCGG TGTACCAGCA AAATGTCCTG
    CATACTGGGA AGAGGTGGTT TATCATCTGT CCGGTGGCCG AGCACCCAGC AGTAGAGGCC
    CCCGAATCTT CACCCCCACT TCCTCTGAGC TCCCTGCAGC CAGACACCAG CCAAGATTCA
    GCACCCTCTA AAGACCAGCT ATCCTCCAAG GAGAAACCCA GCTTCTCAAC TGATCTGCAG
    CTTCTGAGCC AAGCAGGCCC CAGGGACCTT CCTGGTGGTG CACCAGCCCC TTTGGTGCCA
    TCATCCCCTC CCGTGACTGC TCTGTCCCAA GACATGGCCC CACCAGCTGC CAGTCCTAAG
    CTGGAGCCAG CAACGGGGCC TGCCATGCAG GCAGGGGGAC CAGGAACCCC TCAGGGGCCA
    GCCAGTGAGC ATAAGACTCC CTGGCCATTA ATGGAAACTC TTGATGCTCA GCTTGCTGCA
    CAGTCCCCTG GCATGGGCCG AGGGCCTTGT GCCCCACCTT TGGAGGCCCC CCTTCACCCC
    ATGGGTCTGG GGGATCCTGC CTTAGCCAGA GAGCCCATCA CCAAGGGGGA GCCCCTGTCA
    GCTTTGGCCC CCACATCTGG TGCCCCTGGC GGGGCCTCTC AGCCCACTGT GAGCCAGCCC
    CTTCCCCCAC TCCCCACTGT GGTGGGAGCA ATCAGTCTGG CCACTCCCCA GCTCCCCAGT
    CCCCCGCTGG CACCCACTGC CCCACCTCAG CCGCCTTCAA CCTTGGAGTC AGATGGAGAA
    GGGCCGCCCC CAAGGGTGGG CTTTGTGGAC AGCACCATCA AGAGCCTGGA CGAGAAGCTG
    AGGACTCTGC TCTACCAGGA GCACGTGCCC ACCTCCTCAG CCTCCGCCGG GACCCCTGTA
    GAGGCAGGTG ACCGAGACTT CACCCTGGAA CCCCCAAGAG GGGATGAGCC CCGTACAGAG
    GCCTCCGGGG GCAGTCTGGG CCTGCTGCCC CAACCTGCGT TGGAAAAGTC AGAAGTGGCC
    CCTGGATTAG AGGTCACACT GGAGCAGTCC GTGTCCTTGT CAGTGACAGC TGAATCATCT
    TTGAGCCACA CACTTGGCCG TGAGGGAGCA CAAGTGGCCT CAGATTCGCG CACAGCACCC
    CGTGCCCCCC AGGATGTCCC TGCTTCTGCC ATCCCTGCCC ATCTGGAGCC CACAGACCGG
    ACCAGCAGGG TCCCTGGGGA AACCTTGGCT GAGTCCATGC AGAGCTGCCT GGGGATGTTG
    ATTGAGGGTG GCCAGGACAG CCAACCCCAG CCACACAGCA CTCTGGTATC AGGATCCCCT
    CTGAAGTGGC CAGGGCAGCA GGATGGCAGC TCGCCAGCCA AAACCGTGGG CCGGTTCTCA
    GTGGTCAGCA CCCAGGATGA GTGGACTCTG GCCTCCCCCA ATAGCCTGCG GTACTCAGCC
    CCACCTGACG TTTACCTGGA CGAGGCTCCC TGCAGCCCAG ACATGAAGCT GGCTGTGCGG
    CGGGCACAGA CGGCCTCCTC CATCGAGGTC GGCATGGAAG AGCCTTTGTC CAGTGACTCT
    GGGGATGAGT CCCCTCGCAG GAGGCCTTCT GTGCAGAAGC ATGCCTCCCT GCCCAGCAGC
    AGTGGTGGTG TGGCCAGTGA CTTTGTGAAG AAGGCCGCTG CCTTCCTGCA GAGGTCCTCT
    CGGGCTGGCT CCCCATGCCT GGAGACACCC AGCAGGATGG GTGTGAAGGT CCCCACCATC
    AGCGTGACCT CCTTCCACTC CCAGTCATCC TACATCAGCA GTGACAACGA CTCAGAGATT
    GAGGATGCTG ATATCAGGAA AGAGCTGCAG AGTCTTCGGG AGAAGCATCT GAAGGAGATC
    TCAGAGCTAC AAAGTCAGCA GAAACAGGAG ATTGAGGCCC TGTACCGCCG CCTAGGCAAG
    CCGCTACCCC CCAACTTAGG CCTCTTCCAC ACGGCCCCAC CCATTGGCCG CCGGAGAAAG
    ACCAGCAAGA GCAAGCTGAA GGCGGGGAAG TTGCTGAGCC CCCTGGTGCA GCAGCTCAAA
    GTCGTGGCCT CCAGCACAGG TCACTTGTCA GACTGCAGCA GAGCCCCTCC TGCTAAGGAC
    CCTGCCCAAG CTGGGGCAGG GCCCACAGCA GCTTCAGACC CATGTGGGAA GGCAGTTCAG
    ACTCAGCAGC CTTGCTCCGT GCGGGCCTCC CTGTCTGCAG ACATCTGCTC CGGCTTGGCC
    AGTGATGGAG GCGGAGCGCA CGGCCCAGGC TGGACGGTTT ACCACCCAAC GTCTGAGAGA
    GTGACCTATA AGTCTAGTAG CAAACCTCGC GCTCGATTCC TCAGTGGACC CGTGTCTGTG
    TCCATCTGGT CTGCCTTGAA GCGTCTCTGT CTAGGCAAAG AGCACAGCAG TCGGTCCTCC
    ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG GGCCCCCAAC CTGCCCTGCA CATCCAGGCG
    CAGGTGAACA ACAGCAACAA CAAGAAAGGC ACCTTCACAG ACGATCTGCA CAAGCTGGTG
    GACGAGTGGA CAAGCAAGAC GGTCGGGGCC ACACCACTAA AGCCGTCACT CAACCAGTTG
    AAGCAGACGC AGAAATTGCA TGACATGGAG GCCACAGCAG GCTGGCCCCT GGCCTCTGGC
    GAGGCACGGG CTATGAACAC ATCTCGAACA GCAATGGGGA TGCCATGTCT GGCCCCGGTA
    CCCAGCCCTG TACCCACCGT GGTCATTCCT GGAGCTGCCT CAGCCCTTCC TGTGCCCATG
    CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199334.2
    CDS210..6419
    Translation

    Target ORF information:

    RefSeq Version XM_005199277.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199277.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGGAGCAGGC CTCACAGGAC
    AAGCAGCCCA GCCTCCCTCA GAGCTGTGAG AGCTATGGAG GCTCTGACGT CCCTTCTGGA
    AGAGAGATGA GTGACAGCTG CGAAGGTGCA TTTGGTGGGG GAAAGCTGGA AGGCAAGGCC
    GCCCGGAAAC ATCACCGCAG GTCCACGCGT GTGCGCTCCC GCCAGGAGAG GGCCAGTCGG
    CCCCGACTGA CCATCCTGAA CGTGTGCAAC ACGGGTGACA AGATGGTGGA GTGCCAGTTG
    GAGACACACA ACCACAAGAT GGTGACGTTC AAGTTTGACC TGGATGGGGA TGCGCCTGAC
    GAGATCGCTA CCTACATGGT AGAGCACGAC TTCATCCTGC AGGCCGAGCG GGAGACATTC
    ATTGAGCAGA TGAAGGATGT CATGGACAAG GCAGAGGATA TGCTCAGTGA GGACACGGAT
    GTGGACCGTG CCTCCGACCC TGGGGTGAGC CCGCCACACC TCAGCACCTG TGGCCTGGGT
    GCTGGGGAGG AGAATCGCCA ATCCCAAGCA AACGCCCCGG TGTACCAGCA AAATGTCCTG
    CATACTGGGA AGAGGTGGTT TATCATCTGT CCGGTGGCCG AGCACCCAGC AGTAGAGGCC
    CCCGAATCTT CACCCCCACT TCCTCTGAGC TCCCTGCAGC CAGACACCAG CCAAGATTCA
    GCACCCTCTA AAGACCAGCT ATCCTCCAAG GAGAAACCCA GCTTCTCAAC TGATCTGCAG
    CTTCTGAGCC AAGCAGGCCC CAGGGACCTT CCTGGTGGTG CACCAGCCCC TTTGGTGCCA
    TCATCCCCTC CCGTGACTGC TCTGTCCCAA GACATGGCCC CACCAGCTGC CAGTCCTAAG
    CTGGAGCCAG CAACGGGGCC TGCCATGCAG GCAGGGGGAC CAGGAACCCC TCAGGGGCCA
    GCCAGTGAGC ATAAGACTCC CTGGCCATTA ATGGAAACTC TTGATGCTCA GCTTGCTGCA
    CAGTCCCCTG GCATGGGCCG AGGGCCTTGT GCCCCACCTT TGGAGGCCCC CCTTCACCCC
    ATGGGTCTGG GGGATCCTGC CTTAGCCAGA GAGCCCATCA CCAAGGGGGA GCCCCTGTCA
    GCTTTGGCCC CCACATCTGG TGCCCCTGGC GGGGCCTCTC AGCCCACTGT GAGCCAGCCC
    CTTCCCCCAC TCCCCACTGT GGTGGGAGCA ATCAGTCTGG CCACTCCCCA GCTCCCCAGT
    CCCCCGCTGG CACCCACTGC CCCACCTCAG CCGCCTTCAA CCTTGGAGTC AGATGGAGAA
    GGGCCGCCCC CAAGGGTGGG CTTTGTGGAC AGCACCATCA AGAGCCTGGA CGAGAAGCTG
    AGGACTCTGC TCTACCAGGA GCACGTGCCC ACCTCCTCAG CCTCCGCCGG GACCCCTGTA
    GAGGCAGGTG ACCGAGACTT CACCCTGGAA CCCCCAAGAG GGGATGAGCC CCGTACAGAG
    GCCTCCGGGG GCAGTCTGGG CCTGCTGCCC CAACCTGCGT TGGAAAAGTC AGAAGTGGCC
    CCTGGATTAG AGGTCACACT GGAGCAGTCC GTGTCCTTGT CAGTGACAGC TGAATCATCT
    TTGAGCCACA CACTTGGCCG TGAGGGAGCA CAAGTGGCCT CAGATTCGCG CACAGCACCC
    CGTGCCCCCC AGGATGTCCC TGCTTCTGCC ATCCCTGCCC ATCTGGAGCC CACAGACCGG
    ACCAGCAGGG TCCCTGGGGA AACCTTGGCT GAGTCCATGC AGAGCTGCCT GGGGATGTTG
    ATTGAGGGTG GCCAGGACAG CCAACCCCAG CCACACAGCA CTCTGGTATC AGGATCCCCT
    CTGAAGTGGC CAGGGCAGCA GGATGGCAGC TCGCCAGCCA AAACCGTGGG CCGGTTCTCA
    GTGGTCAGCA CCCAGGATGA GTGGACTCTG GCCTCCCCCA ATAGCCTGCG GTACTCAGCC
    CCACCTGACG TTTACCTGGA CGAGGCTCCC TGCAGCCCAG ACATGAAGCT GGCTGTGCGG
    CGGGCACAGA CGGCCTCCTC CATCGAGGTC GGCATGGAAG AGCCTTTGTC CAGTGACTCT
    GGGGATGAGT CCCCTCGCAG GAGGCCTTCT GTGCAGAAGC ATGCCTCCCT GCCCAGCAGC
    AGTGGTGGTG TGGCCAGTGA CTTTGTGAAG AAGGCCGCTG CCTTCCTGCA GAGGTCCTCT
    CGGGCTGGCT CCCCATGCCT GGAGACACCC AGCAGGATGG GTGTGAAGGT CCCCACCATC
    AGCGTGACCT CCTTCCACTC CCAGTCATCC TACATCAGCA GTGACAACGA CTCAGAGATT
    GAGGATGCTG ATATCAGGAA AGAGCTGCAG AGTCTTCGGG AGAAGCATCT GAAGGAGATC
    TCAGAGCTAC AAAGTCAGCA GAAACAGGAG ATTGAGGCCC TGTACCGCCG CCTAGGCAAG
    CCGCTACCCC CCAACTTAGG CCTCTTCCAC ACGGCCCCAC CCATTGGCCG CCGGAGAAAG
    ACCAGCAAGA GCAAGCTGAA GGCGGGGAAG TTGCTGAGCC CCCTGGTGCA GCAGCTCAAA
    GTCGTGGCCT CCAGCACAGG TCACTTGTCA GACTGCAGCA GAGCCCCTCC TGCTAAGGAC
    CCTGCCCAAG CTGGGGCAGG GCCCACAGCA GCTTCAGACC CATGTGGGAA GGCAGTTCAG
    ACTCAGCAGC CTTGCTCCGT GCGGGCCTCC CTGTCTGCAG ACATCTGCTC CGGCTTGGCC
    AGTGATGGAG GCGGAGCGCA CGGCCCAGGC TGGACGGTTT ACCACCCAAC GTCTGAGAGA
    GTGACCTATA AGTCTAGTAG CAAACCTCGC GCTCGATTCC TCAGTGGACC CGTGTCTGTG
    TCCATCTGGT CTGCCTTGAA GCGTCTCTGT CTAGGCAAAG AGCACAGCAG TCGGTCCTCC
    ACCAGCAGCC TGGTCCCGGG CCCTGAGCCG GGCCCCCAAC CTGCCCTGCA CATCCAGGCG
    CAGGTGAACA ACAGCAACAA CAAGAAAGGC ACCTTCACAG ACGATCTGCA CAAGCTGGTG
    GACGAGTGGA CAAGCAAGAC GGTCGGGGCC ACACCACTAA AGCCGTCACT CAACCAGTTG
    AAGCAGACGC AGAAATTGCA TGACATGGAG GCCACAGCAG GCTGGCCCCT GGCCTCTGGC
    GAGGCACGGG CTATGAACAC ATCTCGAACA GCAATGGGGA TGCCATGTCT GGCCCCGGTA
    CCCAGCCCTG TACCCACCGT GGTCATTCCT GGAGCTGCCT CAGCCCTTCC TGTGCCCATG
    CCAGATTCTG AAAGTGAGAA GCCCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22302
    Clone ID Related Accession (Same CDS sequence) XM_005210451.3 , XM_005199278.3
    Accession Version XM_005210451.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCCTC CACCAGCAGC CTGGTCCCGG GCCCTGAGCC GGGCCCCCAA
    CCTGCCCTGC ACATCCAGGC GCAGGTGAAC AACAGCAACA ACAAGAAAGG CACCTTCACA
    GACGATCTGC ACAAGCTGGT GGACGAGTGG ACAAGCAAGA CGGTCGGGGC CACACCACTA
    AAGCCGTCAC TCAACCAGTT GAAGCAGACG CAGAAATTGC ATGACATGGA GGCCACAGCA
    GGCTGGCCCC TGGCCTCTGG CGAGGCACGG GCTATGAACA CATCTCGAAC AGCAATGGGG
    ATGCCATGTC TGGCCCCGGT ACCCAGCCCT GTACCCACCG TGGTCATTCC TGGAGCTGCC
    TCAGCCCTTC CTGTGCCCAT GCCAGATTCT GAAAGTGAGA AGCCCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210508.1
    CDS211..6381
    Translation

    Target ORF information:

    RefSeq Version XM_005210451.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210451.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCCTC CACCAGCAGC CTGGTCCCGG GCCCTGAGCC GGGCCCCCAA
    CCTGCCCTGC ACATCCAGGC GCAGGTGAAC AACAGCAACA ACAAGAAAGG CACCTTCACA
    GACGATCTGC ACAAGCTGGT GGACGAGTGG ACAAGCAAGA CGGTCGGGGC CACACCACTA
    AAGCCGTCAC TCAACCAGTT GAAGCAGACG CAGAAATTGC ATGACATGGA GGCCACAGCA
    GGCTGGCCCC TGGCCTCTGG CGAGGCACGG GCTATGAACA CATCTCGAAC AGCAATGGGG
    ATGCCATGTC TGGCCCCGGT ACCCAGCCCT GTACCCACCG TGGTCATTCC TGGAGCTGCC
    TCAGCCCTTC CTGTGCCCAT GCCAGATTCT GAAAGTGAGA AGCCCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22302
    Clone ID Related Accession (Same CDS sequence) XM_005210451.3 , XM_005199278.3
    Accession Version XM_005199278.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCCTC CACCAGCAGC CTGGTCCCGG GCCCTGAGCC GGGCCCCCAA
    CCTGCCCTGC ACATCCAGGC GCAGGTGAAC AACAGCAACA ACAAGAAAGG CACCTTCACA
    GACGATCTGC ACAAGCTGGT GGACGAGTGG ACAAGCAAGA CGGTCGGGGC CACACCACTA
    AAGCCGTCAC TCAACCAGTT GAAGCAGACG CAGAAATTGC ATGACATGGA GGCCACAGCA
    GGCTGGCCCC TGGCCTCTGG CGAGGCACGG GCTATGAACA CATCTCGAAC AGCAATGGGG
    ATGCCATGTC TGGCCCCGGT ACCCAGCCCT GTACCCACCG TGGTCATTCC TGGAGCTGCC
    TCAGCCCTTC CTGTGCCCAT GCCAGATTCT GAAAGTGAGA AGCCCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199335.2
    CDS211..6381
    Translation

    Target ORF information:

    RefSeq Version XM_005199278.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199278.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGACGACG ATGGCGGCCG CCGAAACGCC CCGGGCGCAC TGATGGAGGC CGGGCGCGGT 
    GCGGGTCCCG CGGGCATGGC GGAGCCCGGG GCACTGCCGC GGGTGCGGGC GGCGCGGCCG
    GGGCCCCAGC GCTTCCTGCG GCGCAGCGTG GTGGAGTCTG ATCAGGAGGA GCCGCCGGGC
    CTGGAGGCTG CCGAGGCGCC GGGCGCGCAG CCCCCGCAGC CGCTGCAGCG CAGGGTGCTG
    CTGCTATGCA AGACGCGCCG CCTCATCGCC GAGCGCGCCC GAGGTCGCCC GGCCGCCCCA
    TTGCCTGCCG CGCCTGCTGC GCCGCCGAGC AACCTTGCCG ACCCCGGCCT GGAGCCGGCC
    GGCGCGCAGG AACCCGGCCC GGACCCCGCG CCAGCTCCGG TGCCCGCCCC CGACGGCGGT
    CCCCGTGAGG AGATAGCGGC CACGAGGAAG GAGGACGCGG GGGCTCCCGA GGCCAGGCCG
    GAGCTCGGGC GCGCACGTCG GGAGGAGCCC GAGGAGGAGG AAGACGACGA GGACGACCTG
    AAGGCTGTGG CCACTTCCCT GGATGGCCGG TTCCTCAAGT TTGACATTGA GCTTGGCCGC
    GGCTCTTTCA AGACGGTCTA TAAAGGGCTG GATACGGAGA CCTGGGTGGA GGTAGCCTGG
    TGCGAGCTGC AGGACCGGAA GCTCACCAAA CTGGAGAGGC AGCGGTTCAA GGAAGAGGCT
    GAGATGCTGA AGGGCCTGCA GCACCCCAAC ATTGTGCGCT TCTATGACTT CTGGGAGTCC
    AGTGCCAGGG GGAAGCGGTG CATCGTGCTG GTGACTGAAC TGATGACCTC GGGGACACTG
    AAGACGTATC TGAAGCGATT CAAGGTGATG AAGCCCAAGG TTCTACGTAG CTGGTGCCGG
    CAGATCCTGA AGGGCCTGCT GTTCCTGCAC ACCAGGACGC CCCCCATCAT CCACCGGGAC
    CTCAAGTGTG ACAACATCTT TATCACTGGG CCCACAGGGT CTGTGAAGAT TGGCGACTTG
    GGCCTGGCCA CCCTCAAGAG AGCATCCTTT GCTAAAAGCG TGATAGGTAC CCCCGAGTTC
    ATGGCACCAG AGATGTACGA GGAGCACTAC GATGAGTCCG TGGACGTGTA CGCCTTTGGG
    ATGTGCATGT TGGAGATGGC CACCTCGGAG TACCCCTACT CAGAGTGCCA GAATGCCGCC
    CAGATCTACA GGAAGGTCAC CTGTGGCATC AAGCCAGCCA GCTTTGAGAA AGTGCACGAT
    CCTGAAATCA AGGAGATTAT TGGGGAGTGT ATCTGCAAAA ACAAGGAGGA AAGGTATGAG
    ATCAAGGACC TGCTCAGCCA CGCCTTCTTC GCGGAGGACA CAGGTGTGAG GGTGGAGCTG
    GCCGAGGAGG ACCATGGCAG GAAGTCTGCC ATTGCCCTGC GGCTCTGGGT TGAGGACCCT
    AAGAAACTGA AGGGCAAGCC CAAGGACAGC GGGGCCATAG AGTTCACCTT CGACCTGGAG
    AGGGAGACGC CAGAAGACGT GGCACAGGAA ATGATTGAGT CAGGATTTTT CCATGAGAGT
    GACGTGAAGA TTGTGGCCAA GTCCATCCGT GACCGTGTGG CATTGATCCA GTGGCGGCGA
    AAGAGGATCT GGCCAGCGCT GCAGCCCCAG GAGCAGCGGG ACCTCGGCAG CCCTGACAAG
    GCCAGGGGCC CACCCACGCC CCTGCAGGTC CAGGTGACCT ACCACTCACA GGCCGGGCTG
    CCAGAGTCCG AGGAGCCCGA GGCCGACCAG CACCTCCTCA CCCCTGCGCT GCCGGCCAGC
    GCCACCTCCC TGGCCTCGGA CAGCACGTTC GACAGTGGCC AGGGCTCAAC GGTGTATTCA
    GACTCGCAGA GTAGCCAGCA GAGTGTTGTG CTGGGGTCGC TGGCAGACGG AGCACCACCC
    GCCTCCCAGT GTGTGTGCAG CCCCCCTGTG AGCGTGAGTG ATGGGCCCGT CCTGCCACAT
    AGCCTGCCCT CCCTAGGGGC CTACCAGCAG CCTGCCACGC CTGGCCTGTC GGTGGGCTCT
    GTCCCACCCC CCGCCCGCCC TCCGCTCCCA CAGCAGCCTT TCCCAGAGCC AGCCATGAGC
    TTCGCCCCTG TACTGCCACC GCCCAGTGCC CCTGTGCCGA CAGGCCCAGG CCAGCCCACA
    GCCCCCGCCC ACCAGCCTTC TCTCCTGGCC CAGCCGGTAC CCTTGCCGCA GGTCCTGGCC
    CCACAACCCG TGGGCCCTCT GCAGCCAGCG CCCTCCCACC TGCCTCCGTA CCTGGCTCCA
    GCCTCCCAGG TGGTGGCCCC TGCTCAGCTG AAGCCCCTCC AGATGCCTCC GGCGCCCCTG
    CAGCCACTAG CTCAGATCCC CCCGCAGATG CCTCCACTTC CTGTTGTCCC CCCCATCGCT
    CCACTGGCCA CAGTTGACAG CCTCCCCACA GCCCTTCCTG ACCTGCCAGC CACCAGTGGG
    CCCACCGTGC CTCCCCCCTC TCAGTATTTC CCTCCAGCTG TCATCTTGCC AAGCCTCCCA
    CTCAGTGCCA CCCCCGCTCC CCTGCCTGCG TCGCCGGCTC TGCCTCTGCA GACAGTCAAG
    CTGCCTCACC CTGCTGGGGC ACCGCTGGCC GTGCCGTGCC AGACCATCGT GCCAAATGTG
    GCGGCCACTG CCACCGCCAT CCCTCTGCTG GCTGTGGCCC CACAGGGCGT GGCCGCCCTG
    TCTATTCACC CCACTGTGGC CCAGCTCCCG GCGCAGCCCG TGTACCCAGC GGCCTTTCCG
    CAGATGGTGC CCAGCGACGT CCCGCCTTCT CCCCTCCACG CAGCGCAGAC TGTGCAGGCT
    GCCGCTCCCC AGCCGACACC CCCCGTGCTG CCACAGCCCC ACCAGCCAAG TGTTGCCCGC
    CTCCCGGAGC AGGCTGCTCC GGCTGCCAGT GCTGGAAGCC AGATCCTGCT TGGCCACCCG
    CCTACATATG CTGTGGATGT CACCACCCAG GTCGCCACAG TGCCCGCGCC TGCCGCCATT
    CTCTCCCCAC CTCTGCCGGA AGTGCTGCTG CCTCCTGTCC CTGAGCTCCT GCCTCAGCTC
    CCCGGCTCTC TGGCCCCCAC AGTGATAGCA GCTCCTCAGA GCGTGCCTGT CCAGACCGCT
    GCGCTCCTGC CACCTGCTAA TCTGCCGCTG CCCAGTGGGC CTGGGATCCC TGGTCCCTGC
    CCGACCGTTC AGCTGACGGT GGAGCCAGCC CCCGAGGAGC AGGCCTCACA GGACAAGCAG
    CCCAGCCTCC CTCAGAGCTG TGAGAGCTAT GGAGGCTCTG ACGTCCCTTC TGGAAGAGAG
    ATGAGTGACA GCTGCGAAGG TGCATTTGGT GGGGGAAAGC TGGAAGGCAA GGCCGCCCGG
    AAACATCACC GCAGGTCCAC GCGTGTGCGC TCCCGCCAGG AGAGGGCCAG TCGGCCCCGA
    CTGACCATCC TGAACGTGTG CAACACGGGT GACAAGATGG TGGAGTGCCA GTTGGAGACA
    CACAACCACA AGATGGTGAC GTTCAAGTTT GACCTGGATG GGGATGCGCC TGACGAGATC
    GCTACCTACA TGGTAGAGCA CGACTTCATC CTGCAGGCCG AGCGGGAGAC ATTCATTGAG
    CAGATGAAGG ATGTCATGGA CAAGGCAGAG GATATGCTCA GTGAGGACAC GGATGTGGAC
    CGTGCCTCCG ACCCTGGGGT GAGCCCGCCA CACCTCAGCA CCTGTGGCCT GGGTGCTGGG
    GAGGAGAATC GCCAATCCCA AGCAAACGCC CCGGTGTACC AGCAAAATGT CCTGCATACT
    GGGAAGAGGT GGTTTATCAT CTGTCCGGTG GCCGAGCACC CAGCAGTAGA GGCCCCCGAA
    TCTTCACCCC CACTTCCTCT GAGCTCCCTG CAGCCAGACA CCAGCCAAGA TTCAGCACCC
    TCTAAAGACC AGCTATCCTC CAAGGAGAAA CCCAGCTTCT CAACTGATCT GCAGCTTCTG
    AGCCAAGCAG GCCCCAGGGA CCTTCCTGGT GGTGCACCAG CCCCTTTGGT GCCATCATCC
    CCTCCCGTGA CTGCTCTGTC CCAAGACATG GCCCCACCAG CTGCCAGTCC TAAGCTGGAG
    CCAGCAACGG GGCCTGCCAT GCAGGCAGGG GGACCAGGAA CCCCTCAGGG GCCAGCCAGT
    GAGCATAAGA CTCCCTGGCC ATTAATGGAA ACTCTTGATG CTCAGCTTGC TGCACAGTCC
    CCTGGCATGG GCCGAGGGCC TTGTGCCCCA CCTTTGGAGG CCCCCCTTCA CCCCATGGGT
    CTGGGGGATC CTGCCTTAGC CAGAGAGCCC ATCACCAAGG GGGAGCCCCT GTCAGCTTTG
    GCCCCCACAT CTGGTGCCCC TGGCGGGGCC TCTCAGCCCA CTGTGAGCCA GCCCCTTCCC
    CCACTCCCCA CTGTGGTGGG AGCAATCAGT CTGGCCACTC CCCAGCTCCC CAGTCCCCCG
    CTGGCACCCA CTGCCCCACC TCAGCCGCCT TCAACCTTGG AGTCAGATGG AGAAGGGCCG
    CCCCCAAGGG TGGGCTTTGT GGACAGCACC ATCAAGAGCC TGGACGAGAA GCTGAGGACT
    CTGCTCTACC AGGAGCACGT GCCCACCTCC TCAGCCTCCG CCGGGACCCC TGTAGAGGCA
    GGTGACCGAG ACTTCACCCT GGAACCCCCA AGAGGGGATG AGCCCCGTAC AGAGGCCTCC
    GGGGGCAGTC TGGGCCTGCT GCCCCAACCT GCGTTGGAAA AGTCAGAAGT GGCCCCTGGA
    TTAGAGGTCA CACTGGAGCA GTCCGTGTCC TTGTCAGTGA CAGCTGAATC ATCTTTGAGC
    CACACACTTG GCCGTGAGGG AGCACAAGTG GCCTCAGATT CGCGCACAGC ACCCCGTGCC
    CCCCAGGATG TCCCTGCTTC TGCCATCCCT GCCCATCTGG AGCCCACAGA CCGGACCAGC
    AGGGTCCCTG GGGAAACCTT GGCTGAGTCC ATGCAGAGCT GCCTGGGGAT GTTGATTGAG
    GGTGGCCAGG ACAGCCAACC CCAGCCACAC AGCACTCTGG TATCAGGATC CCCTCTGAAG
    TGGCCAGGGC AGCAGGATGG CAGCTCGCCA GCCAAAACCG TGGGCCGGTT CTCAGTGGTC
    AGCACCCAGG ATGAGTGGAC TCTGGCCTCC CCCAATAGCC TGCGGTACTC AGCCCCACCT
    GACGTTTACC TGGACGAGGC TCCCTGCAGC CCAGACATGA AGCTGGCTGT GCGGCGGGCA
    CAGACGGCCT CCTCCATCGA GGTCGGCATG GAAGAGCCTT TGTCCAGTGA CTCTGGGGAT
    GAGTCCCCTC GCAGGAGGCC TTCTGTGCAG AAGCATGCCT CCCTGCCCAG CAGCAGTGGT
    GGTGTGGCCA GTGACTTTGT GAAGAAGGCC GCTGCCTTCC TGCAGAGGTC CTCTCGGGCT
    GGCTCCCCAT GCCTGGAGAC ACCCAGCAGG ATGGGTGTGA AGGTCCCCAC CATCAGCGTG
    ACCTCCTTCC ACTCCCAGTC ATCCTACATC AGCAGTGACA ACGACTCAGA GATTGAGGAT
    GCTGATATCA GGAAAGAGCT GCAGAGTCTT CGGGAGAAGC ATCTGAAGGA GATCTCAGAG
    CTACAAAGTC AGCAGAAACA GGAGATTGAG GCCCTGTACC GCCGCCTAGG CAAGCCGCTA
    CCCCCCAACT TAGGCCTCTT CCACACGGCC CCACCCATTG GCCGCCGGAG AAAGACCAGC
    AAGAGCAAGC TGAAGGCGGG GAAGTTGCTG AGCCCCCTGG TGCAGCAGCT CAAAGTCGTG
    GCCTCCAGCA CAGGGTCCTC CACCAGCAGC CTGGTCCCGG GCCCTGAGCC GGGCCCCCAA
    CCTGCCCTGC ACATCCAGGC GCAGGTGAAC AACAGCAACA ACAAGAAAGG CACCTTCACA
    GACGATCTGC ACAAGCTGGT GGACGAGTGG ACAAGCAAGA CGGTCGGGGC CACACCACTA
    AAGCCGTCAC TCAACCAGTT GAAGCAGACG CAGAAATTGC ATGACATGGA GGCCACAGCA
    GGCTGGCCCC TGGCCTCTGG CGAGGCACGG GCTATGAACA CATCTCGAAC AGCAATGGGG
    ATGCCATGTC TGGCCCCGGT ACCCAGCCCT GTACCCACCG TGGTCATTCC TGGAGCTGCC
    TCAGCCCTTC CTGTGCCCAT GCCAGATTCT GAAAGTGAGA AGCCCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.