SELENOI cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOI
Entrez Gene ID	506381
Full Name	selenoprotein I
Synonyms	EPT1,SELI,hEPT1
Gene Type	protein-coding
Organism	Bos taurus(Cattle)
Summary	The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 7. [provided by RefSeq, Sep 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001075257.2	NP_001068725.2	ethanolaminephosphotransferase 1
NM_001075257.1	NP_001068725.2	ethanolaminephosphotransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPT1	NP_001140169.1
	EPT1	NP_001129035.1
Gallus gallus (chicken)	EPT1	NP_001026699.2
Caenorhabditis elegans (roundworm)	CELE_F54D7.2	NP_491454.2
Mus musculus (house mouse)	Ept1	NP_081928.2
Rattus norvegicus (Norway rat)	Seli	NP_001128226.2
Drosophila melanogaster (fruit fly)	CG33116	NP_788074.1
Xenopus tropicalis (tropical clawed frog)	ept1	NP_001004832.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008881	XP_319628.3
Homo sapiens (human)	EPT1	NP_277040.1
Canis lupus familiaris (dog)	EPT1	NP_001157958.1
Bos taurus (cattle)	EPT1	NP_001068725.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SELENOI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44131	NM_001075257.2 Latest version!	Bos taurus selenoprotein I (SELENOI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OBa44131	NM_001075257.1	Bos taurus selenoprotein I (SELENOI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOI ( NM_001075257.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001068725.2 Bos taurus selenoprotein I (SELENOI), mRNA.

Price & Availability↑

CloneID	OBa44131
Clone ID Related Accession (Same CDS sequence)	NM_001075257.2 , NM_001075257.1
Accession Version	NM_001075257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Bos taurus(cattle)
Product	ethanolaminephosphotransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC118273.1 and NKLS02000011.1. On Jul 21, 2017 this sequence version replaced NM_001075257.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC118273.1, SRR5571254.44754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGTT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTCTCTCTG TATGTCATGC ACCCATTTTG GAACACTGTA 
GTAAAGGTAT TTCCTACTTG GCTGGCTCCA AATCTGATAA CCTTCTCTGG CTTTCTGCTG 
GTTGTGTTCA ATTTTCTACT TTTGGCATAC TTTGATCCTG ACTTTTATGC TTCAGCACCA 
GGTCACAAGC ATGTACCCGA CTGGGTCTGG ATTGTAGTGG GCATCCTCAA CTTCATGGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCCCGCAGAA CCAATTCCAG CACCCCGCTG 
GGGGAGCTTT TTGACCACGG CCTGGATAGT TGGTCGTGTG TTTACTTTGT TGTCACCGTG 
TATTCCATTT TTGGACGAGG ATCATCTGGT GTCAGTGTTT TCGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCT CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCGGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATCTT TGGTTGTGCA CTGTGCGTGA CTCTTCCAAT GAGTTTGTTA 
AATTTTTTCA GAAGCTATAA AAATAACACG TTGAAACACA ATTCAGTCTA TGAAGCTGTG 
GTTCCCTTCT TTTCTCCATG TTTGCTATTC ATTTTGTCTA CAGCGTGGAT CCTCCTGTCA 
CCTTCAGATA TTTTAGAGTT ACACCCTAGA GTGTTCTACT TAATGGTTGG AACAGCCTTT 
GCCAACAGCA CATGTCAGCT GATTGTTTGT CAAATGAGCA GTACCCGGTG CCCGACTTTG 
AACTGGTTGC TGCTTCCTCT TTTTGTGATT GTCATGGTGG TGAATGTAGG AGTAACCCCT 
TACGTTGAGA GCATTCTCCT GTTTACGCTA ACGGCTGCTT TCACTTTGGC CCACATCCAC 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AACCATTTTC ACATTTACCC CTTCTCCTTG 
AGAAAACCAA ACTCGGATTG ACTAGGAGTA GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068725.2
CDS	88..1281
Translation	MASYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTVVKVFPTWLAPNLITFSGFLLVVFNFLLLAYFDPDFYASAPGHKHVPDWVWIVVGILNFMAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSSGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIFGCALCVTLPMSLLNFFRSYKNNTLKHNSVYEAVVPFFSPCLLFILSTAWILLSPSDILELHPRVFYLMVGTAFANSTCQLIVCQMSSTRCPTLNWLLLPLFVIVMVVNVGVTPYVESILLFTLTAAFTLAHIHYGVRVVKQLSNHFHIYPFSLRKPNSD*LGVEEKNIGL

Target ORF information:

RefSeq Version	NM_001075257.2
Organism	Bos taurus(cattle)
Definition	Bos taurus selenoprotein I (SELENOI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075257.2

ORF Insert Sequence:

ATGGCAAGTT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTCTCTCTG TATGTCATGC ACCCATTTTG GAACACTGTA 
GTAAAGGTAT TTCCTACTTG GCTGGCTCCA AATCTGATAA CCTTCTCTGG CTTTCTGCTG 
GTTGTGTTCA ATTTTCTACT TTTGGCATAC TTTGATCCTG ACTTTTATGC TTCAGCACCA 
GGTCACAAGC ATGTACCCGA CTGGGTCTGG ATTGTAGTGG GCATCCTCAA CTTCATGGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCCCGCAGAA CCAATTCCAG CACCCCGCTG 
GGGGAGCTTT TTGACCACGG CCTGGATAGT TGGTCGTGTG TTTACTTTGT TGTCACCGTG 
TATTCCATTT TTGGACGAGG ATCATCTGGT GTCAGTGTTT TCGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCT CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCGGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATCTT TGGTTGTGCA CTGTGCGTGA CTCTTCCAAT GAGTTTGTTA 
AATTTTTTCA GAAGCTATAA AAATAACACG TTGAAACACA ATTCAGTCTA TGAAGCTGTG 
GTTCCCTTCT TTTCTCCATG TTTGCTATTC ATTTTGTCTA CAGCGTGGAT CCTCCTGTCA 
CCTTCAGATA TTTTAGAGTT ACACCCTAGA GTGTTCTACT TAATGGTTGG AACAGCCTTT 
GCCAACAGCA CATGTCAGCT GATTGTTTGT CAAATGAGCA GTACCCGGTG CCCGACTTTG 
AACTGGTTGC TGCTTCCTCT TTTTGTGATT GTCATGGTGG TGAATGTAGG AGTAACCCCT 
TACGTTGAGA GCATTCTCCT GTTTACGCTA ACGGCTGCTT TCACTTTGGC CCACATCCAC 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AACCATTTTC ACATTTACCC CTTCTCCTTG 
AGAAAACCAA ACTCGGATTG ACTAGGAGTA GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SELENOI ( NM_001075257.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001068725.2 Bos taurus selenoprotein I (SELENOI), mRNA.

Price & Availability↑

CloneID	OBa44131
Clone ID Related Accession (Same CDS sequence)	NM_001075257.2 , NM_001075257.1
Accession Version	NM_001075257.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-24
Organism	Bos taurus(Cattle)
Product	ethanolaminephosphotransferase 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC118273.1. On Oct 4, 2006 this sequence version replaced XM_582819.2. ##Evidence-Data-START## Transcript exon combination :: BC118273.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGTT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTCTCTCTG TATGTCATGC ACCCATTTTG GAACACTGTA 
GTAAAGGTAT TTCCTACTTG GCTGGCTCCA AATCTGATAA CCTTCTCTGG CTTTCTGCTG 
GTTGTGTTCA ATTTTCTACT TTTGGCATAC TTTGATCCTG ACTTTTATGC TTCAGCACCA 
GGTCACAAGC ATGTACCCGA CTGGGTCTGG ATTGTAGTGG GCATCCTCAA CTTCATGGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCCCGCAGAA CCAATTCCAG CACCCCGCTG 
GGGGAGCTTT TTGACCACGG CCTGGATAGT TGGTCGTGTG TTTACTTTGT TGTCACCGTG 
TATTCCATTT TTGGACGAGG ATCATCTGGT GTCAGTGTTT TCGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCT CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCGGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATCTT TGGTTGTGCA CTGTGCGTGA CTCTTCCAAT GAGTTTGTTA 
AATTTTTTCA GAAGCTATAA AAATAACACG TTGAAACACA ATTCAGTCTA TGAAGCTGTG 
GTTCCCTTCT TTTCTCCATG TTTGCTATTC ATTTTGTCTA CAGCGTGGAT CCTCCTGTCA 
CCTTCAGATA TTTTAGAGTT ACACCCTAGA GTGTTCTACT TAATGGTTGG AACAGCCTTT 
GCCAACAGCA CATGTCAGCT GATTGTTTGT CAAATGAGCA GTACCCGGTG CCCGACTTTG 
AACTGGTTGC TGCTTCCTCT TTTTGTGATT GTCATGGTGG TGAATGTAGG AGTAACCCCT 
TACGTTGAGA GCATTCTCCT GTTTACGCTA ACGGCTGCTT TCACTTTGGC CCACATCCAC 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AACCATTTTC ACATTTACCC CTTCTCCTTG 
AGAAAACCAA ACTCGGATTG ACTAGGAGTA GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068725.2
CDS	88..1281
Translation	MASYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTVVKVFPTWLAPNLITFSGFLLVVFNFLLLAYFDPDFYASAPGHKHVPDWVWIVVGILNFMAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSSGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIFGCALCVTLPMSLLNFFRSYKNNTLKHNSVYEAVVPFFSPCLLFILSTAWILLSPSDILELHPRVFYLMVGTAFANSTCQLIVCQMSSTRCPTLNWLLLPLFVIVMVVNVGVTPYVESILLFTLTAAFTLAHIHYGVRVVKQLSNHFHIYPFSLRKPNSD*LGVEEKNIGL

Target ORF information:

RefSeq Version	NM_001075257.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus selenoprotein I (SELENOI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075257.1

ORF Insert Sequence:

ATGGCAAGTT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTCTCTCTG TATGTCATGC ACCCATTTTG GAACACTGTA 
GTAAAGGTAT TTCCTACTTG GCTGGCTCCA AATCTGATAA CCTTCTCTGG CTTTCTGCTG 
GTTGTGTTCA ATTTTCTACT TTTGGCATAC TTTGATCCTG ACTTTTATGC TTCAGCACCA 
GGTCACAAGC ATGTACCCGA CTGGGTCTGG ATTGTAGTGG GCATCCTCAA CTTCATGGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCCCGCAGAA CCAATTCCAG CACCCCGCTG 
GGGGAGCTTT TTGACCACGG CCTGGATAGT TGGTCGTGTG TTTACTTTGT TGTCACCGTG 
TATTCCATTT TTGGACGAGG ATCATCTGGT GTCAGTGTTT TCGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCT CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCGGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATCTT TGGTTGTGCA CTGTGCGTGA CTCTTCCAAT GAGTTTGTTA 
AATTTTTTCA GAAGCTATAA AAATAACACG TTGAAACACA ATTCAGTCTA TGAAGCTGTG 
GTTCCCTTCT TTTCTCCATG TTTGCTATTC ATTTTGTCTA CAGCGTGGAT CCTCCTGTCA 
CCTTCAGATA TTTTAGAGTT ACACCCTAGA GTGTTCTACT TAATGGTTGG AACAGCCTTT 
GCCAACAGCA CATGTCAGCT GATTGTTTGT CAAATGAGCA GTACCCGGTG CCCGACTTTG 
AACTGGTTGC TGCTTCCTCT TTTTGTGATT GTCATGGTGG TGAATGTAGG AGTAACCCCT 
TACGTTGAGA GCATTCTCCT GTTTACGCTA ACGGCTGCTT TCACTTTGGC CCACATCCAC 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AACCATTTTC ACATTTACCC CTTCTCCTTG 
AGAAAACCAA ACTCGGATTG ACTAGGAGTA GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

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SELENOI cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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