FOCAD gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005209890.3	XP_005209947.1	focadhesin isoform X1
XM_024995515.1	XP_024851283.1	focadhesin isoform X3
XM_005209890.4	XP_005209947.2	focadhesin isoform X1
XM_024995512.1	XP_024851280.1	focadhesin isoform X1
XM_024995513.1	XP_024851281.1	focadhesin isoform X2
NM_001206515.1	NP_001193444.1	uncharacterized protein KIAA1797 homolog
XM_005209891.3	XP_005209948.1	focadhesin isoform X2
XM_024995514.1	XP_024851282.1	focadhesin isoform X3
XM_024995511.1	XP_024851279.1	focadhesin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236957	XM_024995512.1 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236957	XM_005209890.4 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236957	XM_024995511.1 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236958	XM_024995513.1 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236959	XM_024995514.1 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa236959	XM_024995515.1 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa01811	NM_001206515.1 Latest version!	Bos taurus KIAA1797 ortholog (KIAA1797), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa21890	XM_005209890.3 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa21891	XM_005209891.3 Latest version!	Bos taurus focadhesin (FOCAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

FOCAD ( XM_005209890.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209947.2 Bos taurus focadhesin (FOCAD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236957
Clone ID Related Accession (Same CDS sequence)	XM_005209890.4 , XM_024995512.1 , XM_024995511.1
Accession Version	XM_005209890.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209890.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGCAG GAGCGCTACA GAGACAAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACTACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CTGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209947.2
CDS	106..5523
Translation	MLDDIRKRFEFPNSLIQSQAVGHLIAAVLKENSFSEKIHQCTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKALMKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQERYRDKHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_005209890.4
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209890.4

ORF Insert Sequence:

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGCAG GAGCGCTACA GAGACAAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACTACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CTGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024995512.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851280.1 Bos taurus focadhesin (FOCAD), transcript variant X3, mRNA.

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CloneID	OBa236957
Clone ID Related Accession (Same CDS sequence)	XM_005209890.4 , XM_024995512.1 , XM_024995511.1
Accession Version	XM_024995512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGCAG GAGCGCTACA GAGACAAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACTACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CTGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851280.1
CDS	342..5759
Translation	MLDDIRKRFEFPNSLIQSQAVGHLIAAVLKENSFSEKIHQCTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKALMKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQERYRDKHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024995512.1
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995512.1

ORF Insert Sequence:

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGCAG GAGCGCTACA GAGACAAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACTACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CTGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024995511.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851279.1 Bos taurus focadhesin (FOCAD), transcript variant X2, mRNA.

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CloneID	OBa236957
Clone ID Related Accession (Same CDS sequence)	XM_005209890.4 , XM_024995512.1 , XM_024995511.1
Accession Version	XM_024995511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGCAG GAGCGCTACA GAGACAAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACTACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CTGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851279.1
CDS	3811..9228
Translation	MLDDIRKRFEFPNSLIQSQAVGHLIAAVLKENSFSEKIHQCTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKALMKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQERYRDKHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024995511.1
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995511.1

ORF Insert Sequence:

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGCAG GAGCGCTACA GAGACAAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACTACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CTGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024995513.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851281.1 Bos taurus focadhesin (FOCAD), transcript variant X4, mRNA.

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CloneID	OBa236958
Clone ID Related Accession (Same CDS sequence)	XM_024995513.1
Accession Version	XM_024995513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGGTCTGAAG TTTCGTGTAT CCAAATTATG 
GCGCCATTTC TATGGTATCT ATATTGTGAA CCACCCCAGT TACAAGAATA TGCTAAACTC 
AGATTAACTC TGCTCAAAGT CTTACTTAAA CCCCGGGTCC CTTGTGACAA GGAACAACCA 
TCAATATTGG AACAACAGAT ACTACAACTG TGTTGTGACA TGGTTCCATG TTTGCAGATC 
AAAGATTTGA TACAGACAAC AGAAGTGATG CTGTTTATTG AAGAAGTATA CTTAAACCTT 
TTGCGTTATC CTGTTTTCTG GAAAATTCAG CTTACCCAGC TGACCCTTCA GCTGCTATGT 
GTCTGTGAAG TCAGCTTAAA CATAACTGGG GAATGCTCAT CTTTAATTCA CCTTTTAGAG 
CACAGTGTTG AACTTCTAAA GGAGGATTTT CCTGTTGAAC CGGTCATAAT TGGAATAGCT 
TTACTACTTC TACAGACTCC AGCAAGTCAA CAGAAGCCAA TCTTAAGTCT AGCTCTGAAG 
CTCCTTTCTT CTGCTGAGAG TCAGAAGATC CCAAAGTCCT CGTTGCTGCT AGTGATGCCA 
CTTCTGCAGA TACTGTCTTC TACTGCCTTG GAAGACTGTA TATCCGTGGA TGAAGAAGGT 
CCCTCTAGGC AGCAGTTGGC TGTAAACCTT TTAGAAATGG TGCAGCAGGA GCGCTACAGA 
GACAAGCACC GAAAGCTCTC CTACAAGCTT GCGTTCCCCA TAACCAGCAT GTATGGTTCA 
ATATTTACAG CCTGGAGGAT CCTTGAAGTA ATGAGAGAAG AGTCTGCGGC AAGTGACTGG 
TTGGCTTCCG TGGAGTCATT GCTCCCTATA ACCACAGTGA TCCCTGCTCA TGTTTTTCTT 
CTGCTGGCTT ACCTCCTTGT TGAAGATAAG GGACGAAATC TTCACCAGAT ACTGAAGGTC 
ACTACAGAAT TAGCCCAAGC CGATTCCTCT CAGGTACCGA ATCTGATTCC AGTTTTGATG 
TTCAAATTAG GAAGACCACT GGCACCTGTG TTATACAATG ATATCTTGTA TACTTTACCT 
ACACTTGGAG TTCACAAGGT GTGCATAGGA CAGATTCTAC GAGTAATTCA ACTTCTTGGA 
ACTACTCCAC AACTAAAAGC TGTCACTTTG CGCTTGTTGA CATCTTTGTG GGAAAAACAG 
GATCGTGTCT ATCCTGAACT GCAGCGTTTC ATGGCCATGT CCGATGTGCC CTCTCTGTCT 
GTGGGCAAGG AAGTCCAATG GGAGAAACTA ATTGCTAAAG CAGCATCAAT CAGAGATATA 
TGTAAGCAAA GGCCATATCA GCATGGTGCA GATATGTTGG CAGCTATTTC CCAGGTGTTG 
AATGAGTGCA CTAAGCCTGA CCAAGCTACT CCAGCAGCCT TGGTGTTACA AGGTCTTCAT 
GCACTCTGCC AAGCTGAGGT GGTTTGTATT CGCTCCACTT GGAATGCTCT CTCCCCAAAG 
CTGAGTTGTG ACACAAGACC TCTCATTTTG AAGACCCTCA GTGAACTGTT TTCTCTGGTT 
CCTTCCTTAA CAGTCAATAC AGCTGAGTAT GAGAATTTTA AAGTTCAAGT CCTCAGCTTC 
CTCTGGAACC ATACTCAAAA CAAGGACCCA ATTGTCGCAA ACGCTGCCTA TAAATCTCTG 
TCACACTTTG GTGCCGGGGA CCACACGATT CTTCATCTGC CTGAAGAGGT AAGACCAGAA 
ATCCAAACTC CTGATGACCT AGATGAAGAT GAAGATGAGG AGGGTGATGA AAAAGAGGTG 
GATCTTTCCA TTCCCGGCTC TTGCTATCTC AAACTTTTGT CACTCACAGC TCCTTCAGTT 
TTACCAGCTT TGGAGGAATT TTTTACATCA CTTGTGAAGC AAGAAATGGT GAATATGCCC 
CGTGGGGTGT ATCACTCTGC ATTGAAAGGA GGTGCCCGCT TAGATCAAGG GAAGACTGTA 
GCAGGAATCC CCAATTTTAT GTTGAAAATG TACGAAACAA ACAAGCAGCC AGGTTTGAAA 
CCTGGCCTTG CGGGTGGTAT GTTATTTTGT TATGATATTT CAATGTATCA GAGTAAAGAT 
GGCAAACCAT TGAATCGACT GATGGCCAGT AGAGGGCGAA GCTTCAAGCA GACCTCACTT 
GCTCTAGTGC ATGAGGTTCA TATCCAGCCT TCCGAGTGGC ACCGGGCAAT TTTCCTCCCC 
CAGGCCTGGC TCGCATATAT GACTCGTGCT TATCATGCCA TTTTACAGGG AAGGATAGGA 
GAACTGGATT TGCAGTTAAA ACATGGAAAA GAAGGACCTG AAGAGGTGCA GTACAAAAAA 
AACACAGCCT GGCTCTGGGT TCGAGACATG TTGACTGATG AAATCACCAA AGCAGCTGCA 
AAAGACAGCC CGGTGGTCAA AGGCAACGCG CTGTTAGCCT TAAGCAGCCT CGCTGTCGTC 
GTTTCCAAAC ATGAAGTCAG CCTCTGCTCA GACTCTGACA GAGTCCTGGA GGTTCAGCCT 
CACTTCCTGT CAGTGAAAGA TTGGGTTTCC ATGATATTTG ATACCCTTCT GGTCATCGTT 
GATAGCCACT ACCAACCCAG AGGACACTTT TTCTCCTCAT TTTATCATAA ATCCTATTCC 
GGTGAAAACA CGGCCAGTGC CATTGCCCGT TCCGCGGCCG CCACGGCTTT GTCTCTCCTT 
GTGCCAATTT TCATTATCTC TTGCAAAGAG AAGGTTGAGG AAATCCTGAA CATGCTGACT 
GCCAGGTTAC CTGGGAAACC AAGGGCTGAC GAGTCTCAAG CCGTGCAAAT CCACATGGGC 
CTCGCACTGG GGATGTTTCT CTCTCGCCTG TGTGAGGAGA AACTCAGTGA CATATCTAGC 
CAACAGATGA ACCTTCTTCT GATGAAGTCT TTGGATGCCC TGGAAAACTG CTGCTTTGAC 
CCTAGTCTTG AATACAACAC GGGCTGTATA CTGGGAGTTG GACTCGTCCT ATCCCTCATG 
AGTCACAGCG GTCAGACAGA GTCCCGAGTT CATGTGGCAG CCTCGCTTCG GAAGCTCTCT 
GCATACCTGG ATGACAGCAG GAGCCAGAGC AGAACCTTCC AGGAGGTCCT TGCCTATACC 
ATCAGCTGTG TATGTACATC TGCATTCAGT GCCGGCATCA TTGAGGCAGT GGAGGCTGAA 
GATATCATGA ACAAGCTTCG ACTGTTAGTG GACAACAGCC AGCAGACTTC AGGTTTTGCC 
CTGGCATTAG GAAACATAAT TCACGGCTTG TCTGTGTGTG GACATGGAAA AGCTGAAGAC 
TTGGGCAACA GATTGCTTCC GGCCTGGATC AAAACTGTCC TAACAGAGGG CGCTCCCACA 
ATGCTTTGCT TGGCAGCTCT TCACGGCATG GTGGCGTTGG TAGGCTCCGA AGGGGATGTA 
ATGCAGCTGA AGTCAGAAGC CATCCAGTCC TCTCAGTCTC AAGGCAGACT TAATGAAGTC 
ATTAGAACTT TAACTCAGGT CATCAGTGTG TCTGGGGTGA TTGGTCTTCA GTCAAATGCC 
ATCTGGCTTC TAGGACATCT TCATCTGTCT ACTCTGTCCT CAAATCAAAG TAGAACCTCG 
GTGCCTACTG ACTACAGCTA TTTGCCTGAA AACAGTTTTA TTAGAGCAGC CATTGGCTTC 
TTCGTTACAG GAGGAAAAAA AGGCCCTGAA TCTGTGCCCC CTTCCCTTCT CAAAGTTGTG 
ATGAAACCCA TAGCAACTGT TGGAGAAAAC CACCAGTATC CTCCTGTGAA CTGGGCTGCT 
CTCCTCTCTC CACTTATGAG GCTAAACTTT GGTGAAGAGA TTCAGCAACT GTGTCTTGAA 
ATCATGGTGA CCCAGGCACA ATCCTCCCAG AATGTCACTA CACTGTTGGG AATGTGGGTG 
ATGCCACCTC TGATCCATGG TCTGAGTCTG AATATCAAGA AATACCTTCT CTTATCTGTG 
CCTTCATGGA TAAAACATGT CTCTGATGAA CAGATCGTGG GTTTTGTGGA AAGCTTAATG 
GTGGCAGTTT TTAAAGCAGC TTCCCCACTC TCCAGTCCTG AGTTACGCCC AAGTGCCTTA 
CAGGGCCTGA GTCAGGCCAT GAAACTGCCC AGCCCTTCCC ACCACCTCTG GAGTCTGCTC 
AGCGAAGCTA CTAGGAAAAT TTTTGACCTC CTGCCAAATA AGATTCGGAG AAATGATCTA 
GAGCTGTATA TCAGTGTAGC AAAGTGCCTC TCGGAAATGA CAGATGATGA AGCCGATCGG 
GTTGCCCAGA TTACTGAGAG TAGCCTAGAA AAGGCTGCCT TTGTCAGGCT CTACTTGGTC 
TCTCAAGGAC GATTCCCCTT GATGGGCTTG ACTGATGTGC TCAGTGTCGC TGTCCAGCAC 
CGTGAAAAAG ACACACTGGC CTGGATGACG CTGCACAGCT TATACCAGGC ACGGATTGTG 
AGCCACGCCA ACACTGGCGT GTTGAAGAGA ATGGAGTGGC TCTTGGAACT GATGGGTTAT 
ATCAGAAATG TTGCTTACCA GTCAACGTCT GTTCAGAACG TGGCTCTTGA CGAGGCTTTG 
GACTTCTTGT TGCTGATATT TGCAGCCGCA GTGGTTGCAT GGGCAGATCA CGCTGCCCCT 
CTCCTCCTTG GCCTCAGTGC CAGTTGGTTG CCATGGCACC AGGAAAATGG CCCAGCTGGG 
CCAGCATCAA GCTTGCTTGG CAGGAGTCCA TTACACAGGG TCACTCTGCA GGAGGCTGTC 
ACTCTCCTTC CCAGTAGCAT GCCTCTTCTG CTGCAGAAAG AGCCATGGAA GGAACAGACC 
CAGAAGTTCA TTGACTGGCT GTTCAGCATC ATGGAAAGCC CTAAGGAAGG ACTCTCAGCA 
AAATCCAAGG ATCTTTTAAA AGCTACCCTG CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG 
AAGAAAGCTG TATGGACCAG AGCCTATGGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851281.1
CDS	106..5421
Translation	MLDDIRKRFEFPNSLIQSQAVGHLIAAVLKENSFSEKIHQCTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKALMKLLQMQAVKEGHSGEKNIQDIYNIRSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQERYRDKHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024995513.1
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995513.1

ORF Insert Sequence:

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGGTCTGAAG TTTCGTGTAT CCAAATTATG 
GCGCCATTTC TATGGTATCT ATATTGTGAA CCACCCCAGT TACAAGAATA TGCTAAACTC 
AGATTAACTC TGCTCAAAGT CTTACTTAAA CCCCGGGTCC CTTGTGACAA GGAACAACCA 
TCAATATTGG AACAACAGAT ACTACAACTG TGTTGTGACA TGGTTCCATG TTTGCAGATC 
AAAGATTTGA TACAGACAAC AGAAGTGATG CTGTTTATTG AAGAAGTATA CTTAAACCTT 
TTGCGTTATC CTGTTTTCTG GAAAATTCAG CTTACCCAGC TGACCCTTCA GCTGCTATGT 
GTCTGTGAAG TCAGCTTAAA CATAACTGGG GAATGCTCAT CTTTAATTCA CCTTTTAGAG 
CACAGTGTTG AACTTCTAAA GGAGGATTTT CCTGTTGAAC CGGTCATAAT TGGAATAGCT 
TTACTACTTC TACAGACTCC AGCAAGTCAA CAGAAGCCAA TCTTAAGTCT AGCTCTGAAG 
CTCCTTTCTT CTGCTGAGAG TCAGAAGATC CCAAAGTCCT CGTTGCTGCT AGTGATGCCA 
CTTCTGCAGA TACTGTCTTC TACTGCCTTG GAAGACTGTA TATCCGTGGA TGAAGAAGGT 
CCCTCTAGGC AGCAGTTGGC TGTAAACCTT TTAGAAATGG TGCAGCAGGA GCGCTACAGA 
GACAAGCACC GAAAGCTCTC CTACAAGCTT GCGTTCCCCA TAACCAGCAT GTATGGTTCA 
ATATTTACAG CCTGGAGGAT CCTTGAAGTA ATGAGAGAAG AGTCTGCGGC AAGTGACTGG 
TTGGCTTCCG TGGAGTCATT GCTCCCTATA ACCACAGTGA TCCCTGCTCA TGTTTTTCTT 
CTGCTGGCTT ACCTCCTTGT TGAAGATAAG GGACGAAATC TTCACCAGAT ACTGAAGGTC 
ACTACAGAAT TAGCCCAAGC CGATTCCTCT CAGGTACCGA ATCTGATTCC AGTTTTGATG 
TTCAAATTAG GAAGACCACT GGCACCTGTG TTATACAATG ATATCTTGTA TACTTTACCT 
ACACTTGGAG TTCACAAGGT GTGCATAGGA CAGATTCTAC GAGTAATTCA ACTTCTTGGA 
ACTACTCCAC AACTAAAAGC TGTCACTTTG CGCTTGTTGA CATCTTTGTG GGAAAAACAG 
GATCGTGTCT ATCCTGAACT GCAGCGTTTC ATGGCCATGT CCGATGTGCC CTCTCTGTCT 
GTGGGCAAGG AAGTCCAATG GGAGAAACTA ATTGCTAAAG CAGCATCAAT CAGAGATATA 
TGTAAGCAAA GGCCATATCA GCATGGTGCA GATATGTTGG CAGCTATTTC CCAGGTGTTG 
AATGAGTGCA CTAAGCCTGA CCAAGCTACT CCAGCAGCCT TGGTGTTACA AGGTCTTCAT 
GCACTCTGCC AAGCTGAGGT GGTTTGTATT CGCTCCACTT GGAATGCTCT CTCCCCAAAG 
CTGAGTTGTG ACACAAGACC TCTCATTTTG AAGACCCTCA GTGAACTGTT TTCTCTGGTT 
CCTTCCTTAA CAGTCAATAC AGCTGAGTAT GAGAATTTTA AAGTTCAAGT CCTCAGCTTC 
CTCTGGAACC ATACTCAAAA CAAGGACCCA ATTGTCGCAA ACGCTGCCTA TAAATCTCTG 
TCACACTTTG GTGCCGGGGA CCACACGATT CTTCATCTGC CTGAAGAGGT AAGACCAGAA 
ATCCAAACTC CTGATGACCT AGATGAAGAT GAAGATGAGG AGGGTGATGA AAAAGAGGTG 
GATCTTTCCA TTCCCGGCTC TTGCTATCTC AAACTTTTGT CACTCACAGC TCCTTCAGTT 
TTACCAGCTT TGGAGGAATT TTTTACATCA CTTGTGAAGC AAGAAATGGT GAATATGCCC 
CGTGGGGTGT ATCACTCTGC ATTGAAAGGA GGTGCCCGCT TAGATCAAGG GAAGACTGTA 
GCAGGAATCC CCAATTTTAT GTTGAAAATG TACGAAACAA ACAAGCAGCC AGGTTTGAAA 
CCTGGCCTTG CGGGTGGTAT GTTATTTTGT TATGATATTT CAATGTATCA GAGTAAAGAT 
GGCAAACCAT TGAATCGACT GATGGCCAGT AGAGGGCGAA GCTTCAAGCA GACCTCACTT 
GCTCTAGTGC ATGAGGTTCA TATCCAGCCT TCCGAGTGGC ACCGGGCAAT TTTCCTCCCC 
CAGGCCTGGC TCGCATATAT GACTCGTGCT TATCATGCCA TTTTACAGGG AAGGATAGGA 
GAACTGGATT TGCAGTTAAA ACATGGAAAA GAAGGACCTG AAGAGGTGCA GTACAAAAAA 
AACACAGCCT GGCTCTGGGT TCGAGACATG TTGACTGATG AAATCACCAA AGCAGCTGCA 
AAAGACAGCC CGGTGGTCAA AGGCAACGCG CTGTTAGCCT TAAGCAGCCT CGCTGTCGTC 
GTTTCCAAAC ATGAAGTCAG CCTCTGCTCA GACTCTGACA GAGTCCTGGA GGTTCAGCCT 
CACTTCCTGT CAGTGAAAGA TTGGGTTTCC ATGATATTTG ATACCCTTCT GGTCATCGTT 
GATAGCCACT ACCAACCCAG AGGACACTTT TTCTCCTCAT TTTATCATAA ATCCTATTCC 
GGTGAAAACA CGGCCAGTGC CATTGCCCGT TCCGCGGCCG CCACGGCTTT GTCTCTCCTT 
GTGCCAATTT TCATTATCTC TTGCAAAGAG AAGGTTGAGG AAATCCTGAA CATGCTGACT 
GCCAGGTTAC CTGGGAAACC AAGGGCTGAC GAGTCTCAAG CCGTGCAAAT CCACATGGGC 
CTCGCACTGG GGATGTTTCT CTCTCGCCTG TGTGAGGAGA AACTCAGTGA CATATCTAGC 
CAACAGATGA ACCTTCTTCT GATGAAGTCT TTGGATGCCC TGGAAAACTG CTGCTTTGAC 
CCTAGTCTTG AATACAACAC GGGCTGTATA CTGGGAGTTG GACTCGTCCT ATCCCTCATG 
AGTCACAGCG GTCAGACAGA GTCCCGAGTT CATGTGGCAG CCTCGCTTCG GAAGCTCTCT 
GCATACCTGG ATGACAGCAG GAGCCAGAGC AGAACCTTCC AGGAGGTCCT TGCCTATACC 
ATCAGCTGTG TATGTACATC TGCATTCAGT GCCGGCATCA TTGAGGCAGT GGAGGCTGAA 
GATATCATGA ACAAGCTTCG ACTGTTAGTG GACAACAGCC AGCAGACTTC AGGTTTTGCC 
CTGGCATTAG GAAACATAAT TCACGGCTTG TCTGTGTGTG GACATGGAAA AGCTGAAGAC 
TTGGGCAACA GATTGCTTCC GGCCTGGATC AAAACTGTCC TAACAGAGGG CGCTCCCACA 
ATGCTTTGCT TGGCAGCTCT TCACGGCATG GTGGCGTTGG TAGGCTCCGA AGGGGATGTA 
ATGCAGCTGA AGTCAGAAGC CATCCAGTCC TCTCAGTCTC AAGGCAGACT TAATGAAGTC 
ATTAGAACTT TAACTCAGGT CATCAGTGTG TCTGGGGTGA TTGGTCTTCA GTCAAATGCC 
ATCTGGCTTC TAGGACATCT TCATCTGTCT ACTCTGTCCT CAAATCAAAG TAGAACCTCG 
GTGCCTACTG ACTACAGCTA TTTGCCTGAA AACAGTTTTA TTAGAGCAGC CATTGGCTTC 
TTCGTTACAG GAGGAAAAAA AGGCCCTGAA TCTGTGCCCC CTTCCCTTCT CAAAGTTGTG 
ATGAAACCCA TAGCAACTGT TGGAGAAAAC CACCAGTATC CTCCTGTGAA CTGGGCTGCT 
CTCCTCTCTC CACTTATGAG GCTAAACTTT GGTGAAGAGA TTCAGCAACT GTGTCTTGAA 
ATCATGGTGA CCCAGGCACA ATCCTCCCAG AATGTCACTA CACTGTTGGG AATGTGGGTG 
ATGCCACCTC TGATCCATGG TCTGAGTCTG AATATCAAGA AATACCTTCT CTTATCTGTG 
CCTTCATGGA TAAAACATGT CTCTGATGAA CAGATCGTGG GTTTTGTGGA AAGCTTAATG 
GTGGCAGTTT TTAAAGCAGC TTCCCCACTC TCCAGTCCTG AGTTACGCCC AAGTGCCTTA 
CAGGGCCTGA GTCAGGCCAT GAAACTGCCC AGCCCTTCCC ACCACCTCTG GAGTCTGCTC 
AGCGAAGCTA CTAGGAAAAT TTTTGACCTC CTGCCAAATA AGATTCGGAG AAATGATCTA 
GAGCTGTATA TCAGTGTAGC AAAGTGCCTC TCGGAAATGA CAGATGATGA AGCCGATCGG 
GTTGCCCAGA TTACTGAGAG TAGCCTAGAA AAGGCTGCCT TTGTCAGGCT CTACTTGGTC 
TCTCAAGGAC GATTCCCCTT GATGGGCTTG ACTGATGTGC TCAGTGTCGC TGTCCAGCAC 
CGTGAAAAAG ACACACTGGC CTGGATGACG CTGCACAGCT TATACCAGGC ACGGATTGTG 
AGCCACGCCA ACACTGGCGT GTTGAAGAGA ATGGAGTGGC TCTTGGAACT GATGGGTTAT 
ATCAGAAATG TTGCTTACCA GTCAACGTCT GTTCAGAACG TGGCTCTTGA CGAGGCTTTG 
GACTTCTTGT TGCTGATATT TGCAGCCGCA GTGGTTGCAT GGGCAGATCA CGCTGCCCCT 
CTCCTCCTTG GCCTCAGTGC CAGTTGGTTG CCATGGCACC AGGAAAATGG CCCAGCTGGG 
CCAGCATCAA GCTTGCTTGG CAGGAGTCCA TTACACAGGG TCACTCTGCA GGAGGCTGTC 
ACTCTCCTTC CCAGTAGCAT GCCTCTTCTG CTGCAGAAAG AGCCATGGAA GGAACAGACC 
CAGAAGTTCA TTGACTGGCT GTTCAGCATC ATGGAAAGCC CTAAGGAAGG ACTCTCAGCA 
AAATCCAAGG ATCTTTTAAA AGCTACCCTG CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG 
AAGAAAGCTG TATGGACCAG AGCCTATGGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024995515.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851283.1 Bos taurus focadhesin (FOCAD), transcript variant X6, mRNA.

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CloneID	OBa236959
Clone ID Related Accession (Same CDS sequence)	XM_024995515.1 , XM_024995514.1
Accession Version	XM_024995515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	focadhesin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTAT TACAAATGCA AGCTGTTAAG GAAGGACACA GCGGGGAGAA GAATATTCAG 
GATATATATA ATATTAGAAA CCATCCTCAG CCTTTGATCA CTGTTCTTGA GCACAGACCT 
GATTGCTGGC CAGTCATTTT GCAGCAGCTG ACAGCTTTTT TCCAACAATG CCTTGAAAGG 
TCTGAAGTTT CGTGTATCCA AATTATGGCG CCATTTCTAT GGTATCTATA TTGTGAACCA 
CCCCAGTTAC AAGAATATGC TAAACTCAGA TTAACTCTGC TCAAAGTCTT ACTTAAACCC 
CGGGTCCCTT GTGACAAGGA ACAACCATCA ATATTGGAAC AACAGATACT ACAACTGTGT 
TGTGACATGG TTCCATGTTT GCAGATCAAA GATTTGATAC AGACAACAGA AGTGATGCTG 
TTTATTGAAG AAGTATACTT AAACCTTTTG CGTTATCCTG TTTTCTGGAA AATTCAGCTT 
ACCCAGCTGA CCCTTCAGCT GCTATGTGTC TGTGAAGTCA GCTTAAACAT AACTGGGGAA 
TGCTCATCTT TAATTCACCT TTTAGAGCAC AGTGTTGAAC TTCTAAAGGA GGATTTTCCT 
GTTGAACCGG TCATAATTGG AATAGCTTTA CTACTTCTAC AGACTCCAGC AAGTCAACAG 
AAGCCAATCT TAAGTCTAGC TCTGAAGCTC CTTTCTTCTG CTGAGAGTCA GAAGATCCCA 
AAGTCCTCGT TGCTGCTAGT GATGCCACTT CTGCAGATAC TGTCTTCTAC TGCCTTGGAA 
GACTGTATAT CCGTGGATGA AGAAGGTCCC TCTAGGCAGC AGTTGGCTGT AAACCTTTTA 
GAAATGGTGC AGCAGGAGCG CTACAGAGAC AAGCACCGAA AGCTCTCCTA CAAGCTTGCG 
TTCCCCATAA CCAGCATGTA TGGTTCAATA TTTACAGCCT GGAGGATCCT TGAAGTAATG 
AGAGAAGAGT CTGCGGCAAG TGACTGGTTG GCTTCCGTGG AGTCATTGCT CCCTATAACC 
ACAGTGATCC CTGCTCATGT TTTTCTTCTG CTGGCTTACC TCCTTGTTGA AGATAAGGGA 
CGAAATCTTC ACCAGATACT GAAGGTCACT ACAGAATTAG CCCAAGCCGA TTCCTCTCAG 
GTACCGAATC TGATTCCAGT TTTGATGTTC AAATTAGGAA GACCACTGGC ACCTGTGTTA 
TACAATGATA TCTTGTATAC TTTACCTACA CTTGGAGTTC ACAAGGTGTG CATAGGACAG 
ATTCTACGAG TAATTCAACT TCTTGGAACT ACTCCACAAC TAAAAGCTGT CACTTTGCGC 
TTGTTGACAT CTTTGTGGGA AAAACAGGAT CGTGTCTATC CTGAACTGCA GCGTTTCATG 
GCCATGTCCG ATGTGCCCTC TCTGTCTGTG GGCAAGGAAG TCCAATGGGA GAAACTAATT 
GCTAAAGCAG CATCAATCAG AGATATATGT AAGCAAAGGC CATATCAGCA TGGTGCAGAT 
ATGTTGGCAG CTATTTCCCA GGTGTTGAAT GAGTGCACTA AGCCTGACCA AGCTACTCCA 
GCAGCCTTGG TGTTACAAGG TCTTCATGCA CTCTGCCAAG CTGAGGTGGT TTGTATTCGC 
TCCACTTGGA ATGCTCTCTC CCCAAAGCTG AGTTGTGACA CAAGACCTCT CATTTTGAAG 
ACCCTCAGTG AACTGTTTTC TCTGGTTCCT TCCTTAACAG TCAATACAGC TGAGTATGAG 
AATTTTAAAG TTCAAGTCCT CAGCTTCCTC TGGAACCATA CTCAAAACAA GGACCCAATT 
GTCGCAAACG CTGCCTATAA ATCTCTGTCA CACTTTGGTG CCGGGGACCA CACGATTCTT 
CATCTGCCTG AAGAGGTAAG ACCAGAAATC CAAACTCCTG ATGACCTAGA TGAAGATGAA 
GATGAGGAGG GTGATGAAAA AGAGGTGGAT CTTTCCATTC CCGGCTCTTG CTATCTCAAA 
CTTTTGTCAC TCACAGCTCC TTCAGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCCCGT GGGGTGTATC ACTCTGCATT GAAAGGAGGT 
GCCCGCTTAG ATCAAGGGAA GACTGTAGCA GGAATCCCCA ATTTTATGTT GAAAATGTAC 
GAAACAAACA AGCAGCCAGG TTTGAAACCT GGCCTTGCGG GTGGTATGTT ATTTTGTTAT 
GATATTTCAA TGTATCAGAG TAAAGATGGC AAACCATTGA ATCGACTGAT GGCCAGTAGA 
GGGCGAAGCT TCAAGCAGAC CTCACTTGCT CTAGTGCATG AGGTTCATAT CCAGCCTTCC 
GAGTGGCACC GGGCAATTTT CCTCCCCCAG GCCTGGCTCG CATATATGAC TCGTGCTTAT 
CATGCCATTT TACAGGGAAG GATAGGAGAA CTGGATTTGC AGTTAAAACA TGGAAAAGAA 
GGACCTGAAG AGGTGCAGTA CAAAAAAAAC ACAGCCTGGC TCTGGGTTCG AGACATGTTG 
ACTGATGAAA TCACCAAAGC AGCTGCAAAA GACAGCCCGG TGGTCAAAGG CAACGCGCTG 
TTAGCCTTAA GCAGCCTCGC TGTCGTCGTT TCCAAACATG AAGTCAGCCT CTGCTCAGAC 
TCTGACAGAG TCCTGGAGGT TCAGCCTCAC TTCCTGTCAG TGAAAGATTG GGTTTCCATG 
ATATTTGATA CCCTTCTGGT CATCGTTGAT AGCCACTACC AACCCAGAGG ACACTTTTTC 
TCCTCATTTT ATCATAAATC CTATTCCGGT GAAAACACGG CCAGTGCCAT TGCCCGTTCC 
GCGGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAATTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG GGCTGACGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTC GCACTGGGGA TGTTTCTCTC TCGCCTGTGT 
GAGGAGAAAC TCAGTGACAT ATCTAGCCAA CAGATGAACC TTCTTCTGAT GAAGTCTTTG 
GATGCCCTGG AAAACTGCTG CTTTGACCCT AGTCTTGAAT ACAACACGGG CTGTATACTG 
GGAGTTGGAC TCGTCCTATC CCTCATGAGT CACAGCGGTC AGACAGAGTC CCGAGTTCAT 
GTGGCAGCCT CGCTTCGGAA GCTCTCTGCA TACCTGGATG ACAGCAGGAG CCAGAGCAGA 
ACCTTCCAGG AGGTCCTTGC CTATACCATC AGCTGTGTAT GTACATCTGC ATTCAGTGCC 
GGCATCATTG AGGCAGTGGA GGCTGAAGAT ATCATGAACA AGCTTCGACT GTTAGTGGAC 
AACAGCCAGC AGACTTCAGG TTTTGCCCTG GCATTAGGAA ACATAATTCA CGGCTTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAACAGAT TGCTTCCGGC CTGGATCAAA 
ACTGTCCTAA CAGAGGGCGC TCCCACAATG CTTTGCTTGG CAGCTCTTCA CGGCATGGTG 
GCGTTGGTAG GCTCCGAAGG GGATGTAATG CAGCTGAAGT CAGAAGCCAT CCAGTCCTCT 
CAGTCTCAAG GCAGACTTAA TGAAGTCATT AGAACTTTAA CTCAGGTCAT CAGTGTGTCT 
GGGGTGATTG GTCTTCAGTC AAATGCCATC TGGCTTCTAG GACATCTTCA TCTGTCTACT 
CTGTCCTCAA ATCAAAGTAG AACCTCGGTG CCTACTGACT ACAGCTATTT GCCTGAAAAC 
AGTTTTATTA GAGCAGCCAT TGGCTTCTTC GTTACAGGAG GAAAAAAAGG CCCTGAATCT 
GTGCCCCCTT CCCTTCTCAA AGTTGTGATG AAACCCATAG CAACTGTTGG AGAAAACCAC 
CAGTATCCTC CTGTGAACTG GGCTGCTCTC CTCTCTCCAC TTATGAGGCT AAACTTTGGT 
GAAGAGATTC AGCAACTGTG TCTTGAAATC ATGGTGACCC AGGCACAATC CTCCCAGAAT 
GTCACTACAC TGTTGGGAAT GTGGGTGATG CCACCTCTGA TCCATGGTCT GAGTCTGAAT 
ATCAAGAAAT ACCTTCTCTT ATCTGTGCCT TCATGGATAA AACATGTCTC TGATGAACAG 
ATCGTGGGTT TTGTGGAAAG CTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTCTCC 
AGTCCTGAGT TACGCCCAAG TGCCTTACAG GGCCTGAGTC AGGCCATGAA ACTGCCCAGC 
CCTTCCCACC ACCTCTGGAG TCTGCTCAGC GAAGCTACTA GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA TGATCTAGAG CTGTATATCA GTGTAGCAAA GTGCCTCTCG 
GAAATGACAG ATGATGAAGC CGATCGGGTT GCCCAGATTA CTGAGAGTAG CCTAGAAAAG 
GCTGCCTTTG TCAGGCTCTA CTTGGTCTCT CAAGGACGAT TCCCCTTGAT GGGCTTGACT 
GATGTGCTCA GTGTCGCTGT CCAGCACCGT GAAAAAGACA CACTGGCCTG GATGACGCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CACGCCAACA CTGGCGTGTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATC AGAAATGTTG CTTACCAGTC AACGTCTGTT 
CAGAACGTGG CTCTTGACGA GGCTTTGGAC TTCTTGTTGC TGATATTTGC AGCCGCAGTG 
GTTGCATGGG CAGATCACGC TGCCCCTCTC CTCCTTGGCC TCAGTGCCAG TTGGTTGCCA 
TGGCACCAGG AAAATGGCCC AGCTGGGCCA GCATCAAGCT TGCTTGGCAG GAGTCCATTA 
CACAGGGTCA CTCTGCAGGA GGCTGTCACT CTCCTTCCCA GTAGCATGCC TCTTCTGCTG 
CAGAAAGAGC CATGGAAGGA ACAGACCCAG AAGTTCATTG ACTGGCTGTT CAGCATCATG 
GAAAGCCCTA AGGAAGGACT CTCAGCAAAA TCCAAGGATC TTTTAAAAGC TACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC CTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851283.1
CDS	154..5256
Translation	MKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQERYRDKHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024995515.1
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995515.1

ORF Insert Sequence:

ATGAAACTAT TACAAATGCA AGCTGTTAAG GAAGGACACA GCGGGGAGAA GAATATTCAG 
GATATATATA ATATTAGAAA CCATCCTCAG CCTTTGATCA CTGTTCTTGA GCACAGACCT 
GATTGCTGGC CAGTCATTTT GCAGCAGCTG ACAGCTTTTT TCCAACAATG CCTTGAAAGG 
TCTGAAGTTT CGTGTATCCA AATTATGGCG CCATTTCTAT GGTATCTATA TTGTGAACCA 
CCCCAGTTAC AAGAATATGC TAAACTCAGA TTAACTCTGC TCAAAGTCTT ACTTAAACCC 
CGGGTCCCTT GTGACAAGGA ACAACCATCA ATATTGGAAC AACAGATACT ACAACTGTGT 
TGTGACATGG TTCCATGTTT GCAGATCAAA GATTTGATAC AGACAACAGA AGTGATGCTG 
TTTATTGAAG AAGTATACTT AAACCTTTTG CGTTATCCTG TTTTCTGGAA AATTCAGCTT 
ACCCAGCTGA CCCTTCAGCT GCTATGTGTC TGTGAAGTCA GCTTAAACAT AACTGGGGAA 
TGCTCATCTT TAATTCACCT TTTAGAGCAC AGTGTTGAAC TTCTAAAGGA GGATTTTCCT 
GTTGAACCGG TCATAATTGG AATAGCTTTA CTACTTCTAC AGACTCCAGC AAGTCAACAG 
AAGCCAATCT TAAGTCTAGC TCTGAAGCTC CTTTCTTCTG CTGAGAGTCA GAAGATCCCA 
AAGTCCTCGT TGCTGCTAGT GATGCCACTT CTGCAGATAC TGTCTTCTAC TGCCTTGGAA 
GACTGTATAT CCGTGGATGA AGAAGGTCCC TCTAGGCAGC AGTTGGCTGT AAACCTTTTA 
GAAATGGTGC AGCAGGAGCG CTACAGAGAC AAGCACCGAA AGCTCTCCTA CAAGCTTGCG 
TTCCCCATAA CCAGCATGTA TGGTTCAATA TTTACAGCCT GGAGGATCCT TGAAGTAATG 
AGAGAAGAGT CTGCGGCAAG TGACTGGTTG GCTTCCGTGG AGTCATTGCT CCCTATAACC 
ACAGTGATCC CTGCTCATGT TTTTCTTCTG CTGGCTTACC TCCTTGTTGA AGATAAGGGA 
CGAAATCTTC ACCAGATACT GAAGGTCACT ACAGAATTAG CCCAAGCCGA TTCCTCTCAG 
GTACCGAATC TGATTCCAGT TTTGATGTTC AAATTAGGAA GACCACTGGC ACCTGTGTTA 
TACAATGATA TCTTGTATAC TTTACCTACA CTTGGAGTTC ACAAGGTGTG CATAGGACAG 
ATTCTACGAG TAATTCAACT TCTTGGAACT ACTCCACAAC TAAAAGCTGT CACTTTGCGC 
TTGTTGACAT CTTTGTGGGA AAAACAGGAT CGTGTCTATC CTGAACTGCA GCGTTTCATG 
GCCATGTCCG ATGTGCCCTC TCTGTCTGTG GGCAAGGAAG TCCAATGGGA GAAACTAATT 
GCTAAAGCAG CATCAATCAG AGATATATGT AAGCAAAGGC CATATCAGCA TGGTGCAGAT 
ATGTTGGCAG CTATTTCCCA GGTGTTGAAT GAGTGCACTA AGCCTGACCA AGCTACTCCA 
GCAGCCTTGG TGTTACAAGG TCTTCATGCA CTCTGCCAAG CTGAGGTGGT TTGTATTCGC 
TCCACTTGGA ATGCTCTCTC CCCAAAGCTG AGTTGTGACA CAAGACCTCT CATTTTGAAG 
ACCCTCAGTG AACTGTTTTC TCTGGTTCCT TCCTTAACAG TCAATACAGC TGAGTATGAG 
AATTTTAAAG TTCAAGTCCT CAGCTTCCTC TGGAACCATA CTCAAAACAA GGACCCAATT 
GTCGCAAACG CTGCCTATAA ATCTCTGTCA CACTTTGGTG CCGGGGACCA CACGATTCTT 
CATCTGCCTG AAGAGGTAAG ACCAGAAATC CAAACTCCTG ATGACCTAGA TGAAGATGAA 
GATGAGGAGG GTGATGAAAA AGAGGTGGAT CTTTCCATTC CCGGCTCTTG CTATCTCAAA 
CTTTTGTCAC TCACAGCTCC TTCAGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCCCGT GGGGTGTATC ACTCTGCATT GAAAGGAGGT 
GCCCGCTTAG ATCAAGGGAA GACTGTAGCA GGAATCCCCA ATTTTATGTT GAAAATGTAC 
GAAACAAACA AGCAGCCAGG TTTGAAACCT GGCCTTGCGG GTGGTATGTT ATTTTGTTAT 
GATATTTCAA TGTATCAGAG TAAAGATGGC AAACCATTGA ATCGACTGAT GGCCAGTAGA 
GGGCGAAGCT TCAAGCAGAC CTCACTTGCT CTAGTGCATG AGGTTCATAT CCAGCCTTCC 
GAGTGGCACC GGGCAATTTT CCTCCCCCAG GCCTGGCTCG CATATATGAC TCGTGCTTAT 
CATGCCATTT TACAGGGAAG GATAGGAGAA CTGGATTTGC AGTTAAAACA TGGAAAAGAA 
GGACCTGAAG AGGTGCAGTA CAAAAAAAAC ACAGCCTGGC TCTGGGTTCG AGACATGTTG 
ACTGATGAAA TCACCAAAGC AGCTGCAAAA GACAGCCCGG TGGTCAAAGG CAACGCGCTG 
TTAGCCTTAA GCAGCCTCGC TGTCGTCGTT TCCAAACATG AAGTCAGCCT CTGCTCAGAC 
TCTGACAGAG TCCTGGAGGT TCAGCCTCAC TTCCTGTCAG TGAAAGATTG GGTTTCCATG 
ATATTTGATA CCCTTCTGGT CATCGTTGAT AGCCACTACC AACCCAGAGG ACACTTTTTC 
TCCTCATTTT ATCATAAATC CTATTCCGGT GAAAACACGG CCAGTGCCAT TGCCCGTTCC 
GCGGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAATTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG GGCTGACGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTC GCACTGGGGA TGTTTCTCTC TCGCCTGTGT 
GAGGAGAAAC TCAGTGACAT ATCTAGCCAA CAGATGAACC TTCTTCTGAT GAAGTCTTTG 
GATGCCCTGG AAAACTGCTG CTTTGACCCT AGTCTTGAAT ACAACACGGG CTGTATACTG 
GGAGTTGGAC TCGTCCTATC CCTCATGAGT CACAGCGGTC AGACAGAGTC CCGAGTTCAT 
GTGGCAGCCT CGCTTCGGAA GCTCTCTGCA TACCTGGATG ACAGCAGGAG CCAGAGCAGA 
ACCTTCCAGG AGGTCCTTGC CTATACCATC AGCTGTGTAT GTACATCTGC ATTCAGTGCC 
GGCATCATTG AGGCAGTGGA GGCTGAAGAT ATCATGAACA AGCTTCGACT GTTAGTGGAC 
AACAGCCAGC AGACTTCAGG TTTTGCCCTG GCATTAGGAA ACATAATTCA CGGCTTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAACAGAT TGCTTCCGGC CTGGATCAAA 
ACTGTCCTAA CAGAGGGCGC TCCCACAATG CTTTGCTTGG CAGCTCTTCA CGGCATGGTG 
GCGTTGGTAG GCTCCGAAGG GGATGTAATG CAGCTGAAGT CAGAAGCCAT CCAGTCCTCT 
CAGTCTCAAG GCAGACTTAA TGAAGTCATT AGAACTTTAA CTCAGGTCAT CAGTGTGTCT 
GGGGTGATTG GTCTTCAGTC AAATGCCATC TGGCTTCTAG GACATCTTCA TCTGTCTACT 
CTGTCCTCAA ATCAAAGTAG AACCTCGGTG CCTACTGACT ACAGCTATTT GCCTGAAAAC 
AGTTTTATTA GAGCAGCCAT TGGCTTCTTC GTTACAGGAG GAAAAAAAGG CCCTGAATCT 
GTGCCCCCTT CCCTTCTCAA AGTTGTGATG AAACCCATAG CAACTGTTGG AGAAAACCAC 
CAGTATCCTC CTGTGAACTG GGCTGCTCTC CTCTCTCCAC TTATGAGGCT AAACTTTGGT 
GAAGAGATTC AGCAACTGTG TCTTGAAATC ATGGTGACCC AGGCACAATC CTCCCAGAAT 
GTCACTACAC TGTTGGGAAT GTGGGTGATG CCACCTCTGA TCCATGGTCT GAGTCTGAAT 
ATCAAGAAAT ACCTTCTCTT ATCTGTGCCT TCATGGATAA AACATGTCTC TGATGAACAG 
ATCGTGGGTT TTGTGGAAAG CTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTCTCC 
AGTCCTGAGT TACGCCCAAG TGCCTTACAG GGCCTGAGTC AGGCCATGAA ACTGCCCAGC 
CCTTCCCACC ACCTCTGGAG TCTGCTCAGC GAAGCTACTA GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA TGATCTAGAG CTGTATATCA GTGTAGCAAA GTGCCTCTCG 
GAAATGACAG ATGATGAAGC CGATCGGGTT GCCCAGATTA CTGAGAGTAG CCTAGAAAAG 
GCTGCCTTTG TCAGGCTCTA CTTGGTCTCT CAAGGACGAT TCCCCTTGAT GGGCTTGACT 
GATGTGCTCA GTGTCGCTGT CCAGCACCGT GAAAAAGACA CACTGGCCTG GATGACGCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CACGCCAACA CTGGCGTGTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATC AGAAATGTTG CTTACCAGTC AACGTCTGTT 
CAGAACGTGG CTCTTGACGA GGCTTTGGAC TTCTTGTTGC TGATATTTGC AGCCGCAGTG 
GTTGCATGGG CAGATCACGC TGCCCCTCTC CTCCTTGGCC TCAGTGCCAG TTGGTTGCCA 
TGGCACCAGG AAAATGGCCC AGCTGGGCCA GCATCAAGCT TGCTTGGCAG GAGTCCATTA 
CACAGGGTCA CTCTGCAGGA GGCTGTCACT CTCCTTCCCA GTAGCATGCC TCTTCTGCTG 
CAGAAAGAGC CATGGAAGGA ACAGACCCAG AAGTTCATTG ACTGGCTGTT CAGCATCATG 
GAAAGCCCTA AGGAAGGACT CTCAGCAAAA TCCAAGGATC TTTTAAAAGC TACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC CTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024995514.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851282.1 Bos taurus focadhesin (FOCAD), transcript variant X5, mRNA.

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CloneID	OBa236959
Clone ID Related Accession (Same CDS sequence)	XM_024995515.1 , XM_024995514.1
Accession Version	XM_024995514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	focadhesin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTAT TACAAATGCA AGCTGTTAAG GAAGGACACA GCGGGGAGAA GAATATTCAG 
GATATATATA ATATTAGAAA CCATCCTCAG CCTTTGATCA CTGTTCTTGA GCACAGACCT 
GATTGCTGGC CAGTCATTTT GCAGCAGCTG ACAGCTTTTT TCCAACAATG CCTTGAAAGG 
TCTGAAGTTT CGTGTATCCA AATTATGGCG CCATTTCTAT GGTATCTATA TTGTGAACCA 
CCCCAGTTAC AAGAATATGC TAAACTCAGA TTAACTCTGC TCAAAGTCTT ACTTAAACCC 
CGGGTCCCTT GTGACAAGGA ACAACCATCA ATATTGGAAC AACAGATACT ACAACTGTGT 
TGTGACATGG TTCCATGTTT GCAGATCAAA GATTTGATAC AGACAACAGA AGTGATGCTG 
TTTATTGAAG AAGTATACTT AAACCTTTTG CGTTATCCTG TTTTCTGGAA AATTCAGCTT 
ACCCAGCTGA CCCTTCAGCT GCTATGTGTC TGTGAAGTCA GCTTAAACAT AACTGGGGAA 
TGCTCATCTT TAATTCACCT TTTAGAGCAC AGTGTTGAAC TTCTAAAGGA GGATTTTCCT 
GTTGAACCGG TCATAATTGG AATAGCTTTA CTACTTCTAC AGACTCCAGC AAGTCAACAG 
AAGCCAATCT TAAGTCTAGC TCTGAAGCTC CTTTCTTCTG CTGAGAGTCA GAAGATCCCA 
AAGTCCTCGT TGCTGCTAGT GATGCCACTT CTGCAGATAC TGTCTTCTAC TGCCTTGGAA 
GACTGTATAT CCGTGGATGA AGAAGGTCCC TCTAGGCAGC AGTTGGCTGT AAACCTTTTA 
GAAATGGTGC AGCAGGAGCG CTACAGAGAC AAGCACCGAA AGCTCTCCTA CAAGCTTGCG 
TTCCCCATAA CCAGCATGTA TGGTTCAATA TTTACAGCCT GGAGGATCCT TGAAGTAATG 
AGAGAAGAGT CTGCGGCAAG TGACTGGTTG GCTTCCGTGG AGTCATTGCT CCCTATAACC 
ACAGTGATCC CTGCTCATGT TTTTCTTCTG CTGGCTTACC TCCTTGTTGA AGATAAGGGA 
CGAAATCTTC ACCAGATACT GAAGGTCACT ACAGAATTAG CCCAAGCCGA TTCCTCTCAG 
GTACCGAATC TGATTCCAGT TTTGATGTTC AAATTAGGAA GACCACTGGC ACCTGTGTTA 
TACAATGATA TCTTGTATAC TTTACCTACA CTTGGAGTTC ACAAGGTGTG CATAGGACAG 
ATTCTACGAG TAATTCAACT TCTTGGAACT ACTCCACAAC TAAAAGCTGT CACTTTGCGC 
TTGTTGACAT CTTTGTGGGA AAAACAGGAT CGTGTCTATC CTGAACTGCA GCGTTTCATG 
GCCATGTCCG ATGTGCCCTC TCTGTCTGTG GGCAAGGAAG TCCAATGGGA GAAACTAATT 
GCTAAAGCAG CATCAATCAG AGATATATGT AAGCAAAGGC CATATCAGCA TGGTGCAGAT 
ATGTTGGCAG CTATTTCCCA GGTGTTGAAT GAGTGCACTA AGCCTGACCA AGCTACTCCA 
GCAGCCTTGG TGTTACAAGG TCTTCATGCA CTCTGCCAAG CTGAGGTGGT TTGTATTCGC 
TCCACTTGGA ATGCTCTCTC CCCAAAGCTG AGTTGTGACA CAAGACCTCT CATTTTGAAG 
ACCCTCAGTG AACTGTTTTC TCTGGTTCCT TCCTTAACAG TCAATACAGC TGAGTATGAG 
AATTTTAAAG TTCAAGTCCT CAGCTTCCTC TGGAACCATA CTCAAAACAA GGACCCAATT 
GTCGCAAACG CTGCCTATAA ATCTCTGTCA CACTTTGGTG CCGGGGACCA CACGATTCTT 
CATCTGCCTG AAGAGGTAAG ACCAGAAATC CAAACTCCTG ATGACCTAGA TGAAGATGAA 
GATGAGGAGG GTGATGAAAA AGAGGTGGAT CTTTCCATTC CCGGCTCTTG CTATCTCAAA 
CTTTTGTCAC TCACAGCTCC TTCAGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCCCGT GGGGTGTATC ACTCTGCATT GAAAGGAGGT 
GCCCGCTTAG ATCAAGGGAA GACTGTAGCA GGAATCCCCA ATTTTATGTT GAAAATGTAC 
GAAACAAACA AGCAGCCAGG TTTGAAACCT GGCCTTGCGG GTGGTATGTT ATTTTGTTAT 
GATATTTCAA TGTATCAGAG TAAAGATGGC AAACCATTGA ATCGACTGAT GGCCAGTAGA 
GGGCGAAGCT TCAAGCAGAC CTCACTTGCT CTAGTGCATG AGGTTCATAT CCAGCCTTCC 
GAGTGGCACC GGGCAATTTT CCTCCCCCAG GCCTGGCTCG CATATATGAC TCGTGCTTAT 
CATGCCATTT TACAGGGAAG GATAGGAGAA CTGGATTTGC AGTTAAAACA TGGAAAAGAA 
GGACCTGAAG AGGTGCAGTA CAAAAAAAAC ACAGCCTGGC TCTGGGTTCG AGACATGTTG 
ACTGATGAAA TCACCAAAGC AGCTGCAAAA GACAGCCCGG TGGTCAAAGG CAACGCGCTG 
TTAGCCTTAA GCAGCCTCGC TGTCGTCGTT TCCAAACATG AAGTCAGCCT CTGCTCAGAC 
TCTGACAGAG TCCTGGAGGT TCAGCCTCAC TTCCTGTCAG TGAAAGATTG GGTTTCCATG 
ATATTTGATA CCCTTCTGGT CATCGTTGAT AGCCACTACC AACCCAGAGG ACACTTTTTC 
TCCTCATTTT ATCATAAATC CTATTCCGGT GAAAACACGG CCAGTGCCAT TGCCCGTTCC 
GCGGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAATTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG GGCTGACGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTC GCACTGGGGA TGTTTCTCTC TCGCCTGTGT 
GAGGAGAAAC TCAGTGACAT ATCTAGCCAA CAGATGAACC TTCTTCTGAT GAAGTCTTTG 
GATGCCCTGG AAAACTGCTG CTTTGACCCT AGTCTTGAAT ACAACACGGG CTGTATACTG 
GGAGTTGGAC TCGTCCTATC CCTCATGAGT CACAGCGGTC AGACAGAGTC CCGAGTTCAT 
GTGGCAGCCT CGCTTCGGAA GCTCTCTGCA TACCTGGATG ACAGCAGGAG CCAGAGCAGA 
ACCTTCCAGG AGGTCCTTGC CTATACCATC AGCTGTGTAT GTACATCTGC ATTCAGTGCC 
GGCATCATTG AGGCAGTGGA GGCTGAAGAT ATCATGAACA AGCTTCGACT GTTAGTGGAC 
AACAGCCAGC AGACTTCAGG TTTTGCCCTG GCATTAGGAA ACATAATTCA CGGCTTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAACAGAT TGCTTCCGGC CTGGATCAAA 
ACTGTCCTAA CAGAGGGCGC TCCCACAATG CTTTGCTTGG CAGCTCTTCA CGGCATGGTG 
GCGTTGGTAG GCTCCGAAGG GGATGTAATG CAGCTGAAGT CAGAAGCCAT CCAGTCCTCT 
CAGTCTCAAG GCAGACTTAA TGAAGTCATT AGAACTTTAA CTCAGGTCAT CAGTGTGTCT 
GGGGTGATTG GTCTTCAGTC AAATGCCATC TGGCTTCTAG GACATCTTCA TCTGTCTACT 
CTGTCCTCAA ATCAAAGTAG AACCTCGGTG CCTACTGACT ACAGCTATTT GCCTGAAAAC 
AGTTTTATTA GAGCAGCCAT TGGCTTCTTC GTTACAGGAG GAAAAAAAGG CCCTGAATCT 
GTGCCCCCTT CCCTTCTCAA AGTTGTGATG AAACCCATAG CAACTGTTGG AGAAAACCAC 
CAGTATCCTC CTGTGAACTG GGCTGCTCTC CTCTCTCCAC TTATGAGGCT AAACTTTGGT 
GAAGAGATTC AGCAACTGTG TCTTGAAATC ATGGTGACCC AGGCACAATC CTCCCAGAAT 
GTCACTACAC TGTTGGGAAT GTGGGTGATG CCACCTCTGA TCCATGGTCT GAGTCTGAAT 
ATCAAGAAAT ACCTTCTCTT ATCTGTGCCT TCATGGATAA AACATGTCTC TGATGAACAG 
ATCGTGGGTT TTGTGGAAAG CTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTCTCC 
AGTCCTGAGT TACGCCCAAG TGCCTTACAG GGCCTGAGTC AGGCCATGAA ACTGCCCAGC 
CCTTCCCACC ACCTCTGGAG TCTGCTCAGC GAAGCTACTA GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA TGATCTAGAG CTGTATATCA GTGTAGCAAA GTGCCTCTCG 
GAAATGACAG ATGATGAAGC CGATCGGGTT GCCCAGATTA CTGAGAGTAG CCTAGAAAAG 
GCTGCCTTTG TCAGGCTCTA CTTGGTCTCT CAAGGACGAT TCCCCTTGAT GGGCTTGACT 
GATGTGCTCA GTGTCGCTGT CCAGCACCGT GAAAAAGACA CACTGGCCTG GATGACGCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CACGCCAACA CTGGCGTGTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATC AGAAATGTTG CTTACCAGTC AACGTCTGTT 
CAGAACGTGG CTCTTGACGA GGCTTTGGAC TTCTTGTTGC TGATATTTGC AGCCGCAGTG 
GTTGCATGGG CAGATCACGC TGCCCCTCTC CTCCTTGGCC TCAGTGCCAG TTGGTTGCCA 
TGGCACCAGG AAAATGGCCC AGCTGGGCCA GCATCAAGCT TGCTTGGCAG GAGTCCATTA 
CACAGGGTCA CTCTGCAGGA GGCTGTCACT CTCCTTCCCA GTAGCATGCC TCTTCTGCTG 
CAGAAAGAGC CATGGAAGGA ACAGACCCAG AAGTTCATTG ACTGGCTGTT CAGCATCATG 
GAAAGCCCTA AGGAAGGACT CTCAGCAAAA TCCAAGGATC TTTTAAAAGC TACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC CTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851282.1
CDS	280..5382
Translation	MKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQERYRDKHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024995514.1
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995514.1

ORF Insert Sequence:

ATGAAACTAT TACAAATGCA AGCTGTTAAG GAAGGACACA GCGGGGAGAA GAATATTCAG 
GATATATATA ATATTAGAAA CCATCCTCAG CCTTTGATCA CTGTTCTTGA GCACAGACCT 
GATTGCTGGC CAGTCATTTT GCAGCAGCTG ACAGCTTTTT TCCAACAATG CCTTGAAAGG 
TCTGAAGTTT CGTGTATCCA AATTATGGCG CCATTTCTAT GGTATCTATA TTGTGAACCA 
CCCCAGTTAC AAGAATATGC TAAACTCAGA TTAACTCTGC TCAAAGTCTT ACTTAAACCC 
CGGGTCCCTT GTGACAAGGA ACAACCATCA ATATTGGAAC AACAGATACT ACAACTGTGT 
TGTGACATGG TTCCATGTTT GCAGATCAAA GATTTGATAC AGACAACAGA AGTGATGCTG 
TTTATTGAAG AAGTATACTT AAACCTTTTG CGTTATCCTG TTTTCTGGAA AATTCAGCTT 
ACCCAGCTGA CCCTTCAGCT GCTATGTGTC TGTGAAGTCA GCTTAAACAT AACTGGGGAA 
TGCTCATCTT TAATTCACCT TTTAGAGCAC AGTGTTGAAC TTCTAAAGGA GGATTTTCCT 
GTTGAACCGG TCATAATTGG AATAGCTTTA CTACTTCTAC AGACTCCAGC AAGTCAACAG 
AAGCCAATCT TAAGTCTAGC TCTGAAGCTC CTTTCTTCTG CTGAGAGTCA GAAGATCCCA 
AAGTCCTCGT TGCTGCTAGT GATGCCACTT CTGCAGATAC TGTCTTCTAC TGCCTTGGAA 
GACTGTATAT CCGTGGATGA AGAAGGTCCC TCTAGGCAGC AGTTGGCTGT AAACCTTTTA 
GAAATGGTGC AGCAGGAGCG CTACAGAGAC AAGCACCGAA AGCTCTCCTA CAAGCTTGCG 
TTCCCCATAA CCAGCATGTA TGGTTCAATA TTTACAGCCT GGAGGATCCT TGAAGTAATG 
AGAGAAGAGT CTGCGGCAAG TGACTGGTTG GCTTCCGTGG AGTCATTGCT CCCTATAACC 
ACAGTGATCC CTGCTCATGT TTTTCTTCTG CTGGCTTACC TCCTTGTTGA AGATAAGGGA 
CGAAATCTTC ACCAGATACT GAAGGTCACT ACAGAATTAG CCCAAGCCGA TTCCTCTCAG 
GTACCGAATC TGATTCCAGT TTTGATGTTC AAATTAGGAA GACCACTGGC ACCTGTGTTA 
TACAATGATA TCTTGTATAC TTTACCTACA CTTGGAGTTC ACAAGGTGTG CATAGGACAG 
ATTCTACGAG TAATTCAACT TCTTGGAACT ACTCCACAAC TAAAAGCTGT CACTTTGCGC 
TTGTTGACAT CTTTGTGGGA AAAACAGGAT CGTGTCTATC CTGAACTGCA GCGTTTCATG 
GCCATGTCCG ATGTGCCCTC TCTGTCTGTG GGCAAGGAAG TCCAATGGGA GAAACTAATT 
GCTAAAGCAG CATCAATCAG AGATATATGT AAGCAAAGGC CATATCAGCA TGGTGCAGAT 
ATGTTGGCAG CTATTTCCCA GGTGTTGAAT GAGTGCACTA AGCCTGACCA AGCTACTCCA 
GCAGCCTTGG TGTTACAAGG TCTTCATGCA CTCTGCCAAG CTGAGGTGGT TTGTATTCGC 
TCCACTTGGA ATGCTCTCTC CCCAAAGCTG AGTTGTGACA CAAGACCTCT CATTTTGAAG 
ACCCTCAGTG AACTGTTTTC TCTGGTTCCT TCCTTAACAG TCAATACAGC TGAGTATGAG 
AATTTTAAAG TTCAAGTCCT CAGCTTCCTC TGGAACCATA CTCAAAACAA GGACCCAATT 
GTCGCAAACG CTGCCTATAA ATCTCTGTCA CACTTTGGTG CCGGGGACCA CACGATTCTT 
CATCTGCCTG AAGAGGTAAG ACCAGAAATC CAAACTCCTG ATGACCTAGA TGAAGATGAA 
GATGAGGAGG GTGATGAAAA AGAGGTGGAT CTTTCCATTC CCGGCTCTTG CTATCTCAAA 
CTTTTGTCAC TCACAGCTCC TTCAGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCCCGT GGGGTGTATC ACTCTGCATT GAAAGGAGGT 
GCCCGCTTAG ATCAAGGGAA GACTGTAGCA GGAATCCCCA ATTTTATGTT GAAAATGTAC 
GAAACAAACA AGCAGCCAGG TTTGAAACCT GGCCTTGCGG GTGGTATGTT ATTTTGTTAT 
GATATTTCAA TGTATCAGAG TAAAGATGGC AAACCATTGA ATCGACTGAT GGCCAGTAGA 
GGGCGAAGCT TCAAGCAGAC CTCACTTGCT CTAGTGCATG AGGTTCATAT CCAGCCTTCC 
GAGTGGCACC GGGCAATTTT CCTCCCCCAG GCCTGGCTCG CATATATGAC TCGTGCTTAT 
CATGCCATTT TACAGGGAAG GATAGGAGAA CTGGATTTGC AGTTAAAACA TGGAAAAGAA 
GGACCTGAAG AGGTGCAGTA CAAAAAAAAC ACAGCCTGGC TCTGGGTTCG AGACATGTTG 
ACTGATGAAA TCACCAAAGC AGCTGCAAAA GACAGCCCGG TGGTCAAAGG CAACGCGCTG 
TTAGCCTTAA GCAGCCTCGC TGTCGTCGTT TCCAAACATG AAGTCAGCCT CTGCTCAGAC 
TCTGACAGAG TCCTGGAGGT TCAGCCTCAC TTCCTGTCAG TGAAAGATTG GGTTTCCATG 
ATATTTGATA CCCTTCTGGT CATCGTTGAT AGCCACTACC AACCCAGAGG ACACTTTTTC 
TCCTCATTTT ATCATAAATC CTATTCCGGT GAAAACACGG CCAGTGCCAT TGCCCGTTCC 
GCGGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAATTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG GGCTGACGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTC GCACTGGGGA TGTTTCTCTC TCGCCTGTGT 
GAGGAGAAAC TCAGTGACAT ATCTAGCCAA CAGATGAACC TTCTTCTGAT GAAGTCTTTG 
GATGCCCTGG AAAACTGCTG CTTTGACCCT AGTCTTGAAT ACAACACGGG CTGTATACTG 
GGAGTTGGAC TCGTCCTATC CCTCATGAGT CACAGCGGTC AGACAGAGTC CCGAGTTCAT 
GTGGCAGCCT CGCTTCGGAA GCTCTCTGCA TACCTGGATG ACAGCAGGAG CCAGAGCAGA 
ACCTTCCAGG AGGTCCTTGC CTATACCATC AGCTGTGTAT GTACATCTGC ATTCAGTGCC 
GGCATCATTG AGGCAGTGGA GGCTGAAGAT ATCATGAACA AGCTTCGACT GTTAGTGGAC 
AACAGCCAGC AGACTTCAGG TTTTGCCCTG GCATTAGGAA ACATAATTCA CGGCTTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAACAGAT TGCTTCCGGC CTGGATCAAA 
ACTGTCCTAA CAGAGGGCGC TCCCACAATG CTTTGCTTGG CAGCTCTTCA CGGCATGGTG 
GCGTTGGTAG GCTCCGAAGG GGATGTAATG CAGCTGAAGT CAGAAGCCAT CCAGTCCTCT 
CAGTCTCAAG GCAGACTTAA TGAAGTCATT AGAACTTTAA CTCAGGTCAT CAGTGTGTCT 
GGGGTGATTG GTCTTCAGTC AAATGCCATC TGGCTTCTAG GACATCTTCA TCTGTCTACT 
CTGTCCTCAA ATCAAAGTAG AACCTCGGTG CCTACTGACT ACAGCTATTT GCCTGAAAAC 
AGTTTTATTA GAGCAGCCAT TGGCTTCTTC GTTACAGGAG GAAAAAAAGG CCCTGAATCT 
GTGCCCCCTT CCCTTCTCAA AGTTGTGATG AAACCCATAG CAACTGTTGG AGAAAACCAC 
CAGTATCCTC CTGTGAACTG GGCTGCTCTC CTCTCTCCAC TTATGAGGCT AAACTTTGGT 
GAAGAGATTC AGCAACTGTG TCTTGAAATC ATGGTGACCC AGGCACAATC CTCCCAGAAT 
GTCACTACAC TGTTGGGAAT GTGGGTGATG CCACCTCTGA TCCATGGTCT GAGTCTGAAT 
ATCAAGAAAT ACCTTCTCTT ATCTGTGCCT TCATGGATAA AACATGTCTC TGATGAACAG 
ATCGTGGGTT TTGTGGAAAG CTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTCTCC 
AGTCCTGAGT TACGCCCAAG TGCCTTACAG GGCCTGAGTC AGGCCATGAA ACTGCCCAGC 
CCTTCCCACC ACCTCTGGAG TCTGCTCAGC GAAGCTACTA GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA TGATCTAGAG CTGTATATCA GTGTAGCAAA GTGCCTCTCG 
GAAATGACAG ATGATGAAGC CGATCGGGTT GCCCAGATTA CTGAGAGTAG CCTAGAAAAG 
GCTGCCTTTG TCAGGCTCTA CTTGGTCTCT CAAGGACGAT TCCCCTTGAT GGGCTTGACT 
GATGTGCTCA GTGTCGCTGT CCAGCACCGT GAAAAAGACA CACTGGCCTG GATGACGCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CACGCCAACA CTGGCGTGTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATC AGAAATGTTG CTTACCAGTC AACGTCTGTT 
CAGAACGTGG CTCTTGACGA GGCTTTGGAC TTCTTGTTGC TGATATTTGC AGCCGCAGTG 
GTTGCATGGG CAGATCACGC TGCCCCTCTC CTCCTTGGCC TCAGTGCCAG TTGGTTGCCA 
TGGCACCAGG AAAATGGCCC AGCTGGGCCA GCATCAAGCT TGCTTGGCAG GAGTCCATTA 
CACAGGGTCA CTCTGCAGGA GGCTGTCACT CTCCTTCCCA GTAGCATGCC TCTTCTGCTG 
CAGAAAGAGC CATGGAAGGA ACAGACCCAG AAGTTCATTG ACTGGCTGTT CAGCATCATG 
GAAAGCCCTA AGGAAGGACT CTCAGCAAAA TCCAAGGATC TTTTAAAAGC TACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC CTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( NM_001206515.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193444.1 Bos taurus KIAA1797 ortholog (KIAA1797), mRNA.

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CloneID	OBa01811
Clone ID Related Accession (Same CDS sequence)	NM_001206515.1
Accession Version	NM_001206515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-11-15
Organism	Bos taurus(cattle)
Product	uncharacterized protein KIAA1797 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03065698.1, AAFC03065654.1, AAFC03018801.1, EE345710.1, EE343139.1, AAFC03018802.1, AAFC03018796.1, AAFC03018795.1, AAFC03082417.1, AAFC03018803.1, BC134538.1, AAFC03082422.1 and AAFC03082420.1. On or before May 4, 2011 this sequence version replaced XM_002689543.1, XM_867351.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_060264.3. COMPLETENESS: complete on the 3' end.

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GCCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGGAG GAGCGCTACA GAGACGAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACCACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTTATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGCCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CCGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCCTCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193444.1
CDS	1..5418
Translation	MLDDIRKRFEFPNSLIQSQAVGHLIAAVLKENSFSEKIHQCTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKALMKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQEERYRDEHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDRVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTLSCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	NM_001206515.1
Organism	Bos taurus(cattle)
Definition	Bos taurus KIAA1797 ortholog (KIAA1797), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206515.1

ORF Insert Sequence:

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GCCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGGAG GAGCGCTACA GAGACGAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACCACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCCTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CAGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTTATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGCCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CCGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCCTCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_005209890.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209947.1 Bos taurus focadhesin (FOCAD), transcript variant X1, mRNA.

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CloneID	OBa21890
Clone ID Related Accession (Same CDS sequence)	XM_005209890.3
Accession Version	XM_005209890.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104082.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GCCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGGAG GAGCGCTACA GAGACGAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACCACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCTTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CGGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CCGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCCGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209947.1
CDS	94..5511
Translation	MLDDIRKRFEFPNSLIQSQAVGHLIAAVLKENSFSEKIHQCTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKALMKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQEERYRDEHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTALSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDGVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_005209890.3
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209890.3

ORF Insert Sequence:

ATGTTGGATG ACATAAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAACAGCT TTTCAGAAAA GATCCACCAG 
TGCACAAACC AGACTCCTGC CTTGAACTTG CTGTGGGAGA AGTGCTGCAG CGATAATGTC 
GTAGTTCGAA CAGCTTGCTG TGAGGGTCTG GTGGCCCTTG TCGCTCAGGA TCACGCGGAG 
TTCAGCTATG TTCTCAATGG GATTCTCAAC CTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCCTTATGAA ACTATTACAA ATGCAAGCTG TTAAGGAAGG ACACAGCGGG 
GAGAAGAATA TTCAGGATAT ATATAATATT AGAAACCATC CTCAGCCTTT GATCACTGTT 
CTTGAGCACA GACCTGATTG CTGGCCAGTC ATTTTGCAGC AGCTGACAGC TTTTTTCCAA 
CAATGCCTTG AAAGGTCTGA AGTTTCGTGT ATCCAAATTA TGGCGCCATT TCTATGGTAT 
CTATATTGTG AACCACCCCA GTTACAAGAA TATGCTAAAC TCAGATTAAC TCTGCTCAAA 
GTCTTACTTA AACCCCGGGT CCCTTGTGAC AAGGAACAAC CATCAATATT GGAACAACAG 
ATACTACAAC TGTGTTGTGA CATGGTTCCA TGTTTGCAGA TCAAAGATTT GATACAGACA 
ACAGAAGTGA TGCTGTTTAT TGAAGAAGTA TACTTAAACC TTTTGCGTTA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GCTGACCCTT CAGCTGCTAT GTGTCTGTGA AGTCAGCTTA 
AACATAACTG GGGAATGCTC ATCTTTAATT CACCTTTTAG AGCACAGTGT TGAACTTCTA 
AAGGAGGATT TTCCTGTTGA ACCGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AACAGAAGCC AATCTTAAGT CTAGCTCTGA AGCTCCTTTC TTCTGCTGAG 
AGTCAGAAGA TCCCAAAGTC CTCGTTGCTG CTAGTGATGC CACTTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCCGTG GATGAAGAAG GCCCCTCTAG GCAGCAGTTG 
GCTGTAAACC TTTTAGAAAT GGTGCAGGAG GAGCGCTACA GAGACGAGCA CCGAAAGCTC 
TCCTACAAGC TTGCGTTCCC CATAACCAGC ATGTATGGTT CAATATTTAC AGCCTGGAGG 
ATCCTTGAAG TAATGAGAGA AGAGTCTGCG GCAAGTGACT GGTTGGCTTC CGTGGAGTCA 
TTGCTCCCTA TAACCACAGT GATCCCTGCT CATGTTTTTC TTCTGCTGGC TTACCTCCTT 
GTTGAAGATA AGGGACGAAA TCTTCACCAG ATACTGAAGG TCACTACAGA ATTAGCCCAA 
GCCGATTCCT CTCAGGTACC GAATCTGATT CCAGTTTTGA TGTTCAAATT AGGAAGACCA 
CTGGCACCTG TGTTATACAA TGATATCTTG TATACTTTAC CTACACTTGG AGTTCACAAG 
GTGTGCATAG GACAGATTCT ACGAGTAATT CAACTTCTTG GAACCACTCC ACAACTAAAA 
GCTGTCACTT TGCGCTTGTT GACATCTTTG TGGGAAAAAC AGGATCGTGT CTATCCTGAA 
CTGCAGCGTT TCATGGCCAT GTCCGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TAATTGCTAA AGCAGCATCA ATCAGAGATA TATGTAAGCA AAGGCCATAT 
CAGCATGGTG CAGATATGTT GGCAGCTATT TCCCAGGTGT TGAATGAGTG CACTAAGCCT 
GACCAAGCTA CTCCAGCAGC CTTGGTGTTA CAAGGTCTTC ATGCACTCTG CCAAGCTGAG 
GTGGTTTGTA TTCGCTCCAC TTGGAATGCT CTCTCCCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCTTCCTT AACAGTCAAT 
ACAGCTGAGT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAA CCATACTCAA 
AACAAGGACC CAATTGTCGC AAACGCTGCC TATAAATCTC TGTCACACTT TGGTGCCGGG 
GACCACACGA TTCTTCATCT GCCTGAAGAG GTAAGACCAG AAATCCAAAC TCCTGATGAC 
CTAGATGAAG ATGAAGATGA GGAGGGTGAT GAAAAAGAGG TGGATCTTTC CATTCCCGGC 
TCTTGCTATC TCAAACTTTT GTCACTCACA GCTCTTTCAG TTTTACCAGC TTTGGAGGAA 
TTTTTTACAT CACTTGTGAA GCAAGAAATG GTGAATATGC CCCGTGGGGT GTATCACTCT 
GCATTGAAAG GAGGTGCCCG CTTAGATCAA GGGAAGACTG TAGCAGGAAT CCCCAATTTT 
ATGTTGAAAA TGTACGAAAC AAACAAGCAG CCAGGTTTGA AACCTGGCCT TGCGGGTGGT 
ATGTTATTTT GTTATGATAT TTCAATGTAT CAGAGTAAAG ATGGCAAACC ATTGAATCGA 
CTGATGGCCA GTAGAGGGCG AAGCTTCAAG CAGACCTCAC TTGCTCTAGT GCATGAGGTT 
CATATCCAGC CTTCCGAGTG GCACCGGGCA ATTTTCCTCC CCCAGGCCTG GCTCGCATAT 
ATGACTCGTG CTTATCATGC CATTTTACAG GGAAGGATAG GAGAACTGGA TTTGCAGTTA 
AAACATGGAA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAAACACAGC CTGGCTCTGG 
GTTCGAGACA TGTTGACTGA TGAAATCACC AAAGCAGCTG CAAAAGACAG CCCGGTGGTC 
AAAGGCAACG CGCTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTTTCCAA ACATGAAGTC 
AGCCTCTGCT CAGACTCTGA CGGAGTCCTG GAGGTTCAGC CTCACTTCCT GTCAGTGAAA 
GATTGGGTTT CCATGATATT TGATACCCTT CTGGTCATCG TTGATAGCCA CTACCAACCC 
AGAGGACACT TTTTCTCCTC ATTTTATCAT AAATCCTATT CCGGTGAAAA CACGGCCAGT 
GCCATTGCCC GTTCCGCGGC CGCCACGGCT TTGTCTCTCC TTGTGCCAAT TTTCATTATC 
TCTTGCAAAG AGAAGGTTGA GGAAATCCTG AACATGCTGA CTGCCAGGTT ACCTGGGAAA 
CCAAGGGCTG ACGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTCGCACT GGGGATGTTT 
CTCTCTCGCC TGTGTGAGGA GAAACTCAGT GACATATCTA GCCAACAGAT GAACCTTCTT 
CTGATGAAGT CTTTGGATGC CCTGGAAAAC TGCTGCTTTG ACCCTAGTCT TGAATACAAC 
ACGGGCTGTA TACTGGGAGT TGGACTCGTC CTATCCCTCA TGAGTCACAG CGGTCAGACA 
GAGTCCCGAG TTCATGTGGC AGCCTCGCTT CGGAAGCTCT CCGCATACCT GGATGACAGC 
AGGAGCCAGA GCAGAACCTT CCAGGAGGTC CTTGCCTATA CCATCAGCTG TGTATGTACA 
TCTGCATTCA GTGCCGGCAT CATTGAGGCA GTGGAGGCTG AAGATATCAT GAACAAGCTT 
CGACTGTTAG TGGACAACAG CCAGCAGACT TCAGGTTTTG CCCTGGCATT AGGAAACATA 
ATTCACGGCT TGTCTGTGTG TGGACATGGA AAAGCTGAAG ACTTGGGCAA CAGATTGCTT 
CCGGCCTGGA TCAAAACTGT CCTAACAGAG GGCGCTCCCA CAATGCTTTG CTTGGCAGCT 
CTTCACGGCA TGGTGGCGTT GGTAGGCTCC GAAGGGGATG TAATGCAGCT GAAGTCAGAA 
GCCATCCAGT CCTCTCAGTC TCAAGGCAGA CTTAATGAAG TCATTAGAAC TTTAACTCAG 
GTCATCAGTG TGTCTGGGGT GATTGGTCTT CAGTCAAATG CCATCTGGCT TCTAGGACAT 
CTTCATCTGT CTACTCTGTC CTCAAATCAA AGTAGAACCT CGGTGCCTAC TGACTACAGC 
TATTTGCCTG AAAACAGTTT TATTAGAGCA GCCATTGGCT TCTTCGTTAC AGGAGGAAAA 
AAAGGCCCTG AATCTGTGCC CCCTTCCCTT CTCAAAGTTG TGATGAAACC CATAGCAACT 
GTTGGAGAAA ACCACCAGTA TCCTCCCGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG 
AGGCTAAACT TTGGTGAAGA GATTCAGCAA CTGTGTCTTG AAATCATGGT GACCCAGGCA 
CAATCCTCCC AGAATGTCAC TACACTGTTG GGAATGTGGG TGATGCCACC TCTGATCCAT 
GGTCTGAGTC TGAATATCAA GAAATACCTT CTCTTATCTG TGCCTTCATG GATAAAACAT 
GTCTCTGATG AACAGATCGT GGGTTTTGTG GAAAGCTTAA TGGTGGCAGT TTTTAAAGCA 
GCTTCCCCAC TCTCCAGTCC TGAGTTACGC CCAAGTGCCT TACAGGGCCT GAGTCAGGCC 
ATGAAACTGC CCAGCCCTTC CCACCACCTC TGGAGTCTGC TCAGCGAAGC TACTAGGAAA 
ATTTTTGACC TCCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGCTGTA TATCAGTGTA 
GCAAAGTGCC TCTCGGAAAT GACAGATGAT GAAGCCGATC GGGTTGCCCA GATTACTGAG 
AGTAGCCTAG AAAAGGCTGC CTTTGTCAGG CTCTACTTGG TCTCTCAAGG ACGATTCCCC 
TTGATGGGCT TGACTGATGT GCTCAGTGTC GCTGTCCAGC ACCGTGAAAA AGACACACTG 
GCCTGGATGA CGCTGCACAG CTTATACCAG GCACGGATTG TGAGCCACGC CAACACTGGC 
GTGTTGAAGA GAATGGAGTG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TGTTGCTTAC 
CAGTCAACGT CTGTTCAGAA CGTGGCTCTT GACGAGGCTT TGGACTTCTT GTTGCTGATA 
TTTGCAGCCG CAGTGGTTGC ATGGGCAGAT CACGCTGCCC CTCTCCTCCT TGGCCTCAGT 
GCCAGTTGGT TGCCATGGCA CCAGGAAAAT GGCCCAGCTG GGCCAGCATC AAGCTTGCTT 
GGCAGGAGTC CATTACACAG GGTCACTCTG CAGGAGGCTG TCACTCTCCT TCCCAGTAGC 
ATGCCTCTTC TGCTGCAGAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG 
CTGTTCAGCA TCATGGAAAG CCCTAAGGAA GGACTCTCAG CAAAATCCAA GGATCTTTTA 
AAAGCTACCC TGCTGTCCTT GAGAGTTCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC 
AGAGCCTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_005209891.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209948.1 Bos taurus focadhesin (FOCAD), transcript variant X2, mRNA.

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CloneID	OBa21891
Clone ID Related Accession (Same CDS sequence)	XM_005209891.3
Accession Version	XM_005209891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104082.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTAT TACAAATGCA AGCTGTTAAG GAAGGACACA GCGGGGAGAA GAATATTCAG 
GATATATATA ATATTAGAAA CCATCCTCAG CCTTTGATCA CTGTTCTTGA GCACAGACCT 
GATTGCTGGC CAGTCATTTT GCAGCAGCTG ACAGCTTTTT TCCAACAATG CCTTGAAAGG 
TCTGAAGTTT CGTGTATCCA AATTATGGCG CCATTTCTAT GGTATCTATA TTGTGAACCA 
CCCCAGTTAC AAGAATATGC TAAACTCAGA TTAACTCTGC TCAAAGTCTT ACTTAAACCC 
CGGGTCCCTT GTGACAAGGA ACAACCATCA ATATTGGAAC AACAGATACT ACAACTGTGT 
TGTGACATGG TTCCATGTTT GCAGATCAAA GATTTGATAC AGACAACAGA AGTGATGCTG 
TTTATTGAAG AAGTATACTT AAACCTTTTG CGTTATCCTG TTTTCTGGAA AATTCAGCTT 
ACCCAGCTGA CCCTTCAGCT GCTATGTGTC TGTGAAGTCA GCTTAAACAT AACTGGGGAA 
TGCTCATCTT TAATTCACCT TTTAGAGCAC AGTGTTGAAC TTCTAAAGGA GGATTTTCCT 
GTTGAACCGG TCATAATTGG AATAGCTTTA CTACTTCTAC AGACTCCAGC AAGTCAACAG 
AAGCCAATCT TAAGTCTAGC TCTGAAGCTC CTTTCTTCTG CTGAGAGTCA GAAGATCCCA 
AAGTCCTCGT TGCTGCTAGT GATGCCACTT CTGCAGATAC TGTCTTCTAC TGCCTTGGAA 
GACTGTATAT CCGTGGATGA AGAAGGCCCC TCTAGGCAGC AGTTGGCTGT AAACCTTTTA 
GAAATGGTGC AGGAGGAGCG CTACAGAGAC GAGCACCGAA AGCTCTCCTA CAAGCTTGCG 
TTCCCCATAA CCAGCATGTA TGGTTCAATA TTTACAGCCT GGAGGATCCT TGAAGTAATG 
AGAGAAGAGT CTGCGGCAAG TGACTGGTTG GCTTCCGTGG AGTCATTGCT CCCTATAACC 
ACAGTGATCC CTGCTCATGT TTTTCTTCTG CTGGCTTACC TCCTTGTTGA AGATAAGGGA 
CGAAATCTTC ACCAGATACT GAAGGTCACT ACAGAATTAG CCCAAGCCGA TTCCTCTCAG 
GTACCGAATC TGATTCCAGT TTTGATGTTC AAATTAGGAA GACCACTGGC ACCTGTGTTA 
TACAATGATA TCTTGTATAC TTTACCTACA CTTGGAGTTC ACAAGGTGTG CATAGGACAG 
ATTCTACGAG TAATTCAACT TCTTGGAACC ACTCCACAAC TAAAAGCTGT CACTTTGCGC 
TTGTTGACAT CTTTGTGGGA AAAACAGGAT CGTGTCTATC CTGAACTGCA GCGTTTCATG 
GCCATGTCCG ATGTGCCCTC TCTGTCTGTG GGCAAGGAAG TCCAATGGGA GAAACTAATT 
GCTAAAGCAG CATCAATCAG AGATATATGT AAGCAAAGGC CATATCAGCA TGGTGCAGAT 
ATGTTGGCAG CTATTTCCCA GGTGTTGAAT GAGTGCACTA AGCCTGACCA AGCTACTCCA 
GCAGCCTTGG TGTTACAAGG TCTTCATGCA CTCTGCCAAG CTGAGGTGGT TTGTATTCGC 
TCCACTTGGA ATGCTCTCTC CCCAAAGCTG AGTTGTGACA CAAGACCTCT CATTTTGAAG 
ACCCTCAGTG AACTGTTTTC TCTGGTTCCT TCCTTAACAG TCAATACAGC TGAGTATGAG 
AATTTTAAAG TTCAAGTCCT CAGCTTCCTC TGGAACCATA CTCAAAACAA GGACCCAATT 
GTCGCAAACG CTGCCTATAA ATCTCTGTCA CACTTTGGTG CCGGGGACCA CACGATTCTT 
CATCTGCCTG AAGAGGTAAG ACCAGAAATC CAAACTCCTG ATGACCTAGA TGAAGATGAA 
GATGAGGAGG GTGATGAAAA AGAGGTGGAT CTTTCCATTC CCGGCTCTTG CTATCTCAAA 
CTTTTGTCAC TCACAGCTCT TTCAGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCCCGT GGGGTGTATC ACTCTGCATT GAAAGGAGGT 
GCCCGCTTAG ATCAAGGGAA GACTGTAGCA GGAATCCCCA ATTTTATGTT GAAAATGTAC 
GAAACAAACA AGCAGCCAGG TTTGAAACCT GGCCTTGCGG GTGGTATGTT ATTTTGTTAT 
GATATTTCAA TGTATCAGAG TAAAGATGGC AAACCATTGA ATCGACTGAT GGCCAGTAGA 
GGGCGAAGCT TCAAGCAGAC CTCACTTGCT CTAGTGCATG AGGTTCATAT CCAGCCTTCC 
GAGTGGCACC GGGCAATTTT CCTCCCCCAG GCCTGGCTCG CATATATGAC TCGTGCTTAT 
CATGCCATTT TACAGGGAAG GATAGGAGAA CTGGATTTGC AGTTAAAACA TGGAAAAGAA 
GGACCTGAAG AGGTGCAGTA CAAAAAAAAC ACAGCCTGGC TCTGGGTTCG AGACATGTTG 
ACTGATGAAA TCACCAAAGC AGCTGCAAAA GACAGCCCGG TGGTCAAAGG CAACGCGCTG 
TTAGCCTTAA GCAGCCTCGC TGTCGTCGTT TCCAAACATG AAGTCAGCCT CTGCTCAGAC 
TCTGACGGAG TCCTGGAGGT TCAGCCTCAC TTCCTGTCAG TGAAAGATTG GGTTTCCATG 
ATATTTGATA CCCTTCTGGT CATCGTTGAT AGCCACTACC AACCCAGAGG ACACTTTTTC 
TCCTCATTTT ATCATAAATC CTATTCCGGT GAAAACACGG CCAGTGCCAT TGCCCGTTCC 
GCGGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAATTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG GGCTGACGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTC GCACTGGGGA TGTTTCTCTC TCGCCTGTGT 
GAGGAGAAAC TCAGTGACAT ATCTAGCCAA CAGATGAACC TTCTTCTGAT GAAGTCTTTG 
GATGCCCTGG AAAACTGCTG CTTTGACCCT AGTCTTGAAT ACAACACGGG CTGTATACTG 
GGAGTTGGAC TCGTCCTATC CCTCATGAGT CACAGCGGTC AGACAGAGTC CCGAGTTCAT 
GTGGCAGCCT CGCTTCGGAA GCTCTCCGCA TACCTGGATG ACAGCAGGAG CCAGAGCAGA 
ACCTTCCAGG AGGTCCTTGC CTATACCATC AGCTGTGTAT GTACATCTGC ATTCAGTGCC 
GGCATCATTG AGGCAGTGGA GGCTGAAGAT ATCATGAACA AGCTTCGACT GTTAGTGGAC 
AACAGCCAGC AGACTTCAGG TTTTGCCCTG GCATTAGGAA ACATAATTCA CGGCTTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAACAGAT TGCTTCCGGC CTGGATCAAA 
ACTGTCCTAA CAGAGGGCGC TCCCACAATG CTTTGCTTGG CAGCTCTTCA CGGCATGGTG 
GCGTTGGTAG GCTCCGAAGG GGATGTAATG CAGCTGAAGT CAGAAGCCAT CCAGTCCTCT 
CAGTCTCAAG GCAGACTTAA TGAAGTCATT AGAACTTTAA CTCAGGTCAT CAGTGTGTCT 
GGGGTGATTG GTCTTCAGTC AAATGCCATC TGGCTTCTAG GACATCTTCA TCTGTCTACT 
CTGTCCTCAA ATCAAAGTAG AACCTCGGTG CCTACTGACT ACAGCTATTT GCCTGAAAAC 
AGTTTTATTA GAGCAGCCAT TGGCTTCTTC GTTACAGGAG GAAAAAAAGG CCCTGAATCT 
GTGCCCCCTT CCCTTCTCAA AGTTGTGATG AAACCCATAG CAACTGTTGG AGAAAACCAC 
CAGTATCCTC CCGTGAACTG GGCTGCTCTC CTCTCTCCAC TTATGAGGCT AAACTTTGGT 
GAAGAGATTC AGCAACTGTG TCTTGAAATC ATGGTGACCC AGGCACAATC CTCCCAGAAT 
GTCACTACAC TGTTGGGAAT GTGGGTGATG CCACCTCTGA TCCATGGTCT GAGTCTGAAT 
ATCAAGAAAT ACCTTCTCTT ATCTGTGCCT TCATGGATAA AACATGTCTC TGATGAACAG 
ATCGTGGGTT TTGTGGAAAG CTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTCTCC 
AGTCCTGAGT TACGCCCAAG TGCCTTACAG GGCCTGAGTC AGGCCATGAA ACTGCCCAGC 
CCTTCCCACC ACCTCTGGAG TCTGCTCAGC GAAGCTACTA GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA TGATCTAGAG CTGTATATCA GTGTAGCAAA GTGCCTCTCG 
GAAATGACAG ATGATGAAGC CGATCGGGTT GCCCAGATTA CTGAGAGTAG CCTAGAAAAG 
GCTGCCTTTG TCAGGCTCTA CTTGGTCTCT CAAGGACGAT TCCCCTTGAT GGGCTTGACT 
GATGTGCTCA GTGTCGCTGT CCAGCACCGT GAAAAAGACA CACTGGCCTG GATGACGCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CACGCCAACA CTGGCGTGTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATC AGAAATGTTG CTTACCAGTC AACGTCTGTT 
CAGAACGTGG CTCTTGACGA GGCTTTGGAC TTCTTGTTGC TGATATTTGC AGCCGCAGTG 
GTTGCATGGG CAGATCACGC TGCCCCTCTC CTCCTTGGCC TCAGTGCCAG TTGGTTGCCA 
TGGCACCAGG AAAATGGCCC AGCTGGGCCA GCATCAAGCT TGCTTGGCAG GAGTCCATTA 
CACAGGGTCA CTCTGCAGGA GGCTGTCACT CTCCTTCCCA GTAGCATGCC TCTTCTGCTG 
CAGAAAGAGC CATGGAAGGA ACAGACCCAG AAGTTCATTG ACTGGCTGTT CAGCATCATG 
GAAAGCCCTA AGGAAGGACT CTCAGCAAAA TCCAAGGATC TTTTAAAAGC TACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC CTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209948.1
CDS	117..5219
Translation	MKLLQMQAVKEGHSGEKNIQDIYNIRNHPQPLITVLEHRPDCWPVILQQLTAFFQQCLERSEVSCIQIMAPFLWYLYCEPPQLQEYAKLRLTLLKVLLKPRVPCDKEQPSILEQQILQLCCDMVPCLQIKDLIQTTEVMLFIEEVYLNLLRYPVFWKIQLTQLTLQLLCVCEVSLNITGECSSLIHLLEHSVELLKEDFPVEPVIIGIALLLLQTPASQQKPILSLALKLLSSAESQKIPKSSLLLVMPLLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQEERYRDEHRKLSYKLAFPITSMYGSIFTAWRILEVMREESAASDWLASVESLLPITTVIPAHVFLLLAYLLVEDKGRNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLAPVLYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLKAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWNHTQNKDPIVANAAYKSLSHFGAGDHTILHLPEEVRPEIQTPDDLDEDEDEEGDEKEVDLSIPGSCYLKLLSLTALSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGARLDQGKTVAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQPSEWHRAIFLPQAWLAYMTRAYHAILQGRIGELDLQLKHGKEGPEEVQYKKNTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEVSLCSDSDGVLEVQPHFLSVKDWVSMIFDTLLVIVDSHYQPRGHFFSSFYHKSYSGENTASAIARSAAATALSLLVPIFIISCKEKVEEILNMLTARLPGKPRADESQAVQIHMGLALGMFLSRLCEEKLSDISSQQMNLLLMKSLDALENCCFDPSLEYNTGCILGVGLVLSLMSHSGQTESRVHVAASLRKLSAYLDDSRSQSRTFQEVLAYTISCVCTSAFSAGIIEAVEAEDIMNKLRLLVDNSQQTSGFALALGNIIHGLSVCGHGKAEDLGNRLLPAWIKTVLTEGAPTMLCLAALHGMVALVGSEGDVMQLKSEAIQSSQSQGRLNEVIRTLTQVISVSGVIGLQSNAIWLLGHLHLSTLSSNQSRTSVPTDYSYLPENSFIRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENHQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNVTTLLGMWVMPPLIHGLSLNIKKYLLLSVPSWIKHVSDEQIVGFVESLMVAVFKAASPLSSPELRPSALQGLSQAMKLPSPSHHLWSLLSEATRKIFDLLPNKIRRNDLELYISVAKCLSEMTDDEADRVAQITESSLEKAAFVRLYLVSQGRFPLMGLTDVLSVAVQHREKDTLAWMTLHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSVQNVALDEALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHQENGPAGPASSLLGRSPLHRVTLQEAVTLLPSSMPLLLQKEPWKEQTQKFIDWLFSIMESPKEGLSAKSKDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_005209891.3
Organism	Bos taurus(cattle)
Definition	Bos taurus focadhesin (FOCAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209891.3

ORF Insert Sequence:

ATGAAACTAT TACAAATGCA AGCTGTTAAG GAAGGACACA GCGGGGAGAA GAATATTCAG 
GATATATATA ATATTAGAAA CCATCCTCAG CCTTTGATCA CTGTTCTTGA GCACAGACCT 
GATTGCTGGC CAGTCATTTT GCAGCAGCTG ACAGCTTTTT TCCAACAATG CCTTGAAAGG 
TCTGAAGTTT CGTGTATCCA AATTATGGCG CCATTTCTAT GGTATCTATA TTGTGAACCA 
CCCCAGTTAC AAGAATATGC TAAACTCAGA TTAACTCTGC TCAAAGTCTT ACTTAAACCC 
CGGGTCCCTT GTGACAAGGA ACAACCATCA ATATTGGAAC AACAGATACT ACAACTGTGT 
TGTGACATGG TTCCATGTTT GCAGATCAAA GATTTGATAC AGACAACAGA AGTGATGCTG 
TTTATTGAAG AAGTATACTT AAACCTTTTG CGTTATCCTG TTTTCTGGAA AATTCAGCTT 
ACCCAGCTGA CCCTTCAGCT GCTATGTGTC TGTGAAGTCA GCTTAAACAT AACTGGGGAA 
TGCTCATCTT TAATTCACCT TTTAGAGCAC AGTGTTGAAC TTCTAAAGGA GGATTTTCCT 
GTTGAACCGG TCATAATTGG AATAGCTTTA CTACTTCTAC AGACTCCAGC AAGTCAACAG 
AAGCCAATCT TAAGTCTAGC TCTGAAGCTC CTTTCTTCTG CTGAGAGTCA GAAGATCCCA 
AAGTCCTCGT TGCTGCTAGT GATGCCACTT CTGCAGATAC TGTCTTCTAC TGCCTTGGAA 
GACTGTATAT CCGTGGATGA AGAAGGCCCC TCTAGGCAGC AGTTGGCTGT AAACCTTTTA 
GAAATGGTGC AGGAGGAGCG CTACAGAGAC GAGCACCGAA AGCTCTCCTA CAAGCTTGCG 
TTCCCCATAA CCAGCATGTA TGGTTCAATA TTTACAGCCT GGAGGATCCT TGAAGTAATG 
AGAGAAGAGT CTGCGGCAAG TGACTGGTTG GCTTCCGTGG AGTCATTGCT CCCTATAACC 
ACAGTGATCC CTGCTCATGT TTTTCTTCTG CTGGCTTACC TCCTTGTTGA AGATAAGGGA 
CGAAATCTTC ACCAGATACT GAAGGTCACT ACAGAATTAG CCCAAGCCGA TTCCTCTCAG 
GTACCGAATC TGATTCCAGT TTTGATGTTC AAATTAGGAA GACCACTGGC ACCTGTGTTA 
TACAATGATA TCTTGTATAC TTTACCTACA CTTGGAGTTC ACAAGGTGTG CATAGGACAG 
ATTCTACGAG TAATTCAACT TCTTGGAACC ACTCCACAAC TAAAAGCTGT CACTTTGCGC 
TTGTTGACAT CTTTGTGGGA AAAACAGGAT CGTGTCTATC CTGAACTGCA GCGTTTCATG 
GCCATGTCCG ATGTGCCCTC TCTGTCTGTG GGCAAGGAAG TCCAATGGGA GAAACTAATT 
GCTAAAGCAG CATCAATCAG AGATATATGT AAGCAAAGGC CATATCAGCA TGGTGCAGAT 
ATGTTGGCAG CTATTTCCCA GGTGTTGAAT GAGTGCACTA AGCCTGACCA AGCTACTCCA 
GCAGCCTTGG TGTTACAAGG TCTTCATGCA CTCTGCCAAG CTGAGGTGGT TTGTATTCGC 
TCCACTTGGA ATGCTCTCTC CCCAAAGCTG AGTTGTGACA CAAGACCTCT CATTTTGAAG 
ACCCTCAGTG AACTGTTTTC TCTGGTTCCT TCCTTAACAG TCAATACAGC TGAGTATGAG 
AATTTTAAAG TTCAAGTCCT CAGCTTCCTC TGGAACCATA CTCAAAACAA GGACCCAATT 
GTCGCAAACG CTGCCTATAA ATCTCTGTCA CACTTTGGTG CCGGGGACCA CACGATTCTT 
CATCTGCCTG AAGAGGTAAG ACCAGAAATC CAAACTCCTG ATGACCTAGA TGAAGATGAA 
GATGAGGAGG GTGATGAAAA AGAGGTGGAT CTTTCCATTC CCGGCTCTTG CTATCTCAAA 
CTTTTGTCAC TCACAGCTCT TTCAGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCCCGT GGGGTGTATC ACTCTGCATT GAAAGGAGGT 
GCCCGCTTAG ATCAAGGGAA GACTGTAGCA GGAATCCCCA ATTTTATGTT GAAAATGTAC 
GAAACAAACA AGCAGCCAGG TTTGAAACCT GGCCTTGCGG GTGGTATGTT ATTTTGTTAT 
GATATTTCAA TGTATCAGAG TAAAGATGGC AAACCATTGA ATCGACTGAT GGCCAGTAGA 
GGGCGAAGCT TCAAGCAGAC CTCACTTGCT CTAGTGCATG AGGTTCATAT CCAGCCTTCC 
GAGTGGCACC GGGCAATTTT CCTCCCCCAG GCCTGGCTCG CATATATGAC TCGTGCTTAT 
CATGCCATTT TACAGGGAAG GATAGGAGAA CTGGATTTGC AGTTAAAACA TGGAAAAGAA 
GGACCTGAAG AGGTGCAGTA CAAAAAAAAC ACAGCCTGGC TCTGGGTTCG AGACATGTTG 
ACTGATGAAA TCACCAAAGC AGCTGCAAAA GACAGCCCGG TGGTCAAAGG CAACGCGCTG 
TTAGCCTTAA GCAGCCTCGC TGTCGTCGTT TCCAAACATG AAGTCAGCCT CTGCTCAGAC 
TCTGACGGAG TCCTGGAGGT TCAGCCTCAC TTCCTGTCAG TGAAAGATTG GGTTTCCATG 
ATATTTGATA CCCTTCTGGT CATCGTTGAT AGCCACTACC AACCCAGAGG ACACTTTTTC 
TCCTCATTTT ATCATAAATC CTATTCCGGT GAAAACACGG CCAGTGCCAT TGCCCGTTCC 
GCGGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAATTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG GGCTGACGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTC GCACTGGGGA TGTTTCTCTC TCGCCTGTGT 
GAGGAGAAAC TCAGTGACAT ATCTAGCCAA CAGATGAACC TTCTTCTGAT GAAGTCTTTG 
GATGCCCTGG AAAACTGCTG CTTTGACCCT AGTCTTGAAT ACAACACGGG CTGTATACTG 
GGAGTTGGAC TCGTCCTATC CCTCATGAGT CACAGCGGTC AGACAGAGTC CCGAGTTCAT 
GTGGCAGCCT CGCTTCGGAA GCTCTCCGCA TACCTGGATG ACAGCAGGAG CCAGAGCAGA 
ACCTTCCAGG AGGTCCTTGC CTATACCATC AGCTGTGTAT GTACATCTGC ATTCAGTGCC 
GGCATCATTG AGGCAGTGGA GGCTGAAGAT ATCATGAACA AGCTTCGACT GTTAGTGGAC 
AACAGCCAGC AGACTTCAGG TTTTGCCCTG GCATTAGGAA ACATAATTCA CGGCTTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAACAGAT TGCTTCCGGC CTGGATCAAA 
ACTGTCCTAA CAGAGGGCGC TCCCACAATG CTTTGCTTGG CAGCTCTTCA CGGCATGGTG 
GCGTTGGTAG GCTCCGAAGG GGATGTAATG CAGCTGAAGT CAGAAGCCAT CCAGTCCTCT 
CAGTCTCAAG GCAGACTTAA TGAAGTCATT AGAACTTTAA CTCAGGTCAT CAGTGTGTCT 
GGGGTGATTG GTCTTCAGTC AAATGCCATC TGGCTTCTAG GACATCTTCA TCTGTCTACT 
CTGTCCTCAA ATCAAAGTAG AACCTCGGTG CCTACTGACT ACAGCTATTT GCCTGAAAAC 
AGTTTTATTA GAGCAGCCAT TGGCTTCTTC GTTACAGGAG GAAAAAAAGG CCCTGAATCT 
GTGCCCCCTT CCCTTCTCAA AGTTGTGATG AAACCCATAG CAACTGTTGG AGAAAACCAC 
CAGTATCCTC CCGTGAACTG GGCTGCTCTC CTCTCTCCAC TTATGAGGCT AAACTTTGGT 
GAAGAGATTC AGCAACTGTG TCTTGAAATC ATGGTGACCC AGGCACAATC CTCCCAGAAT 
GTCACTACAC TGTTGGGAAT GTGGGTGATG CCACCTCTGA TCCATGGTCT GAGTCTGAAT 
ATCAAGAAAT ACCTTCTCTT ATCTGTGCCT TCATGGATAA AACATGTCTC TGATGAACAG 
ATCGTGGGTT TTGTGGAAAG CTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTCTCC 
AGTCCTGAGT TACGCCCAAG TGCCTTACAG GGCCTGAGTC AGGCCATGAA ACTGCCCAGC 
CCTTCCCACC ACCTCTGGAG TCTGCTCAGC GAAGCTACTA GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA TGATCTAGAG CTGTATATCA GTGTAGCAAA GTGCCTCTCG 
GAAATGACAG ATGATGAAGC CGATCGGGTT GCCCAGATTA CTGAGAGTAG CCTAGAAAAG 
GCTGCCTTTG TCAGGCTCTA CTTGGTCTCT CAAGGACGAT TCCCCTTGAT GGGCTTGACT 
GATGTGCTCA GTGTCGCTGT CCAGCACCGT GAAAAAGACA CACTGGCCTG GATGACGCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CACGCCAACA CTGGCGTGTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATC AGAAATGTTG CTTACCAGTC AACGTCTGTT 
CAGAACGTGG CTCTTGACGA GGCTTTGGAC TTCTTGTTGC TGATATTTGC AGCCGCAGTG 
GTTGCATGGG CAGATCACGC TGCCCCTCTC CTCCTTGGCC TCAGTGCCAG TTGGTTGCCA 
TGGCACCAGG AAAATGGCCC AGCTGGGCCA GCATCAAGCT TGCTTGGCAG GAGTCCATTA 
CACAGGGTCA CTCTGCAGGA GGCTGTCACT CTCCTTCCCA GTAGCATGCC TCTTCTGCTG 
CAGAAAGAGC CATGGAAGGA ACAGACCCAG AAGTTCATTG ACTGGCTGTT CAGCATCATG 
GAAAGCCCTA AGGAAGGACT CTCAGCAAAA TCCAAGGATC TTTTAAAAGC TACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC CTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	FOCAD
Entrez Gene ID	506199
Full Name	focadhesin
General protein information	Preferred Names focadhesin Names focadhesin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	FOCAD	NP_060264.3
Bos taurus (cattle)	FOCAD	NP_001193444.1
Rattus norvegicus (Norway rat)	Focad	XP_233144.5
Pan troglodytes (chimpanzee)	FOCAD	XP_001151442.1
	LOC711490	XP_002800166.1
Mus musculus (house mouse)	Focad	NP_001074653.1
Canis lupus familiaris (dog)	FOCAD	XP_538678.2
Gallus gallus (chicken)	FOCAD	NP_001182532.1
Danio rerio (zebrafish)	focad	XP_003198980.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

FOCAD cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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