PRKCQ cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCQ
Entrez Gene ID	505901
Full Name	protein kinase C theta
General protein information	Preferred Names protein kinase C theta Names protein kinase C theta type
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192077.1	NP_001179006.1	protein kinase C theta type
NM_001192077.2	NP_001179006.1	protein kinase C theta type
XM_005214141.2	XP_005214198.1	protein kinase C theta type isoform X1
XM_005214142.3	XP_005214199.1	protein kinase C theta type isoform X1
XM_005214142.4	XP_005214199.1	protein kinase C theta type isoform X1
XM_025000387.1	XP_024856155.1	protein kinase C theta type isoform X1
XM_005214141.3	XP_005214198.1	protein kinase C theta type isoform X1
NM_001192077.3	NP_001179006.1	protein kinase C theta type

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKCQ	NP_006248.1
Pan troglodytes (chimpanzee)	PRKCQ	XP_001148074.1
Canis lupus familiaris (dog)	PRKCQ	XP_005617224.1
Gallus gallus (chicken)	PRKCQ	XP_001235980.2
	PRKCQ	XP_001118689.2
Rattus norvegicus (Norway rat)	Prkcq	NP_001263650.1
Danio rerio (zebrafish)	prkcq	NP_001082839.2
Bos taurus (cattle)	PRKCQ	NP_001179006.1
Mus musculus (house mouse)	Prkcq	NP_032885.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRKCQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa25027	NM_001192077.3 Latest version!	Bos taurus protein kinase C theta (PRKCQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa39240	NM_001192077.1 Latest version!	Bos taurus protein kinase C, theta (PRKCQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa25027	XM_025000387.1 Latest version!	Bos taurus protein kinase C theta (PRKCQ), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa25027	XM_005214141.3 Latest version!	Bos taurus protein kinase C theta (PRKCQ), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa25027	XM_005214142.4 Latest version!	Bos taurus protein kinase C theta (PRKCQ), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa25027	XM_005214142.3	Bos taurus protein kinase C, theta (PRKCQ), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa25027	XM_005214141.2	Bos taurus protein kinase C, theta (PRKCQ), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa25027	NM_001192077.2	Bos taurus protein kinase C theta (PRKCQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCQ ( NM_001192077.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179006.1 Bos taurus protein kinase C theta (PRKCQ), mRNA.

Price & Availability↑

CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	NM_001192077.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	protein kinase C theta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02035339.1. On Jun 30, 2017 this sequence version replaced NM_001192077.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179006.1
CDS	1..2121
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	NM_001192077.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C theta (PRKCQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192077.2

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( NM_001192077.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179006.1 Bos taurus protein kinase C theta (PRKCQ), mRNA.

Price & Availability↑

CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	NM_001192077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Bos taurus(cattle)
Product	protein kinase C theta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000013.1. On Dec 20, 2018 this sequence version replaced NM_001192077.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGVA01009272.1, GGVC01103777.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145418, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179006.1
CDS	1..2121
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	NM_001192077.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C theta (PRKCQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192077.3

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( NM_001192077.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179006.1 Bos taurus protein kinase C, theta (PRKCQ), mRNA.

Price & Availability↑

CloneID	OBa39240
Clone ID Related Accession (Same CDS sequence)	NM_001192077.1
Accession Version	NM_001192077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	protein kinase C theta type
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03066583.1, AAFC02013051.1 and AAFC03006165.1. On Jul 17, 2010 this sequence version replaced XM_582266.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093727, SAMN01093728 [ECO:0000348] ##Evidence-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTACTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGACGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179006.1
CDS	1..2121
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	NM_001192077.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus protein kinase C, theta (PRKCQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192077.1

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTACTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGACGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( XM_005214141.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214198.1 Bos taurus protein kinase C, theta (PRKCQ), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	XM_005214141.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104364.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005214141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214198.1
CDS	50..2170
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	XM_005214141.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C, theta (PRKCQ), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214141.2

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( XM_005214142.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214199.1 Bos taurus protein kinase C, theta (PRKCQ), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	XM_005214142.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104364.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214199.1
CDS	850..2970
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	XM_005214142.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C, theta (PRKCQ), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214142.3

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( XM_005214142.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214199.1 Bos taurus protein kinase C theta (PRKCQ), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	XM_005214142.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214142.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214199.1
CDS	164..2284
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	XM_005214142.4
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C theta (PRKCQ), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214142.4

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( XM_025000387.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856155.1 Bos taurus protein kinase C theta (PRKCQ), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	XM_025000387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856155.1
CDS	37..2157
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	XM_025000387.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C theta (PRKCQ), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000387.1

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCQ ( XM_005214141.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214198.1 Bos taurus protein kinase C theta (PRKCQ), transcript variant X1, mRNA.

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CloneID	OBa25027
Clone ID Related Accession (Same CDS sequence)	NM_001192077.2 , XM_005214141.2 , XM_005214142.3 , XM_005214142.4 , XM_025000387.1 , XM_005214141.3 , NM_001192077.3
Accession Version	XM_005214141.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214198.1
CDS	122..2242
Translation	MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETDSFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCQQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDSEHIFREGPVEIGLPCSIKGEAKPPYVPGPGKKEPQGISWESPLEEMEKVCHRPEPEPNKERGSLRMKLKIEDFTLHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLCRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDARTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLVGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMEKLIS

Target ORF information:

RefSeq Version	XM_005214141.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C theta (PRKCQ), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214141.3

ORF Insert Sequence:

ATGTCACCGT TTCTCCGAAT TGGCTTGTCC AACTTCGACT GTGGGTCCTG CCAGTCCTGC 
CAGGGTGAGG CGGTTAACCC TTACTGTGCC GTGCTTGTCA AAGAGTATGT TGAATCAGAG 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CGCCCTGGGA CAGCACTTTC 
GACGCCCACA TCAACAAGGG ACGAGTGATG CAGATCATCG TGAAGGGCAA AAACGTGGAC 
CTGATCTCCG AGACCACCGT GGAGCTGTAC TCCCTGGCCG AGAGGTGCAG GAAAAACAAC 
GGGAAGACAG AAATATGGCT AGAGCTGAAA CCTCAAGGCC GAATGCTGAT GAACGCAAGA 
TACTTTCTGG AAATGAGTGA TACAAAGGAC ATGAACGAAT TCGAGACCGA CAGCTTCTTC 
GCTCTGCATC ACCGCCGAGG AGCCATCAAG CAGGCCAAAG TCCACCATGT CAAGTGCCAT 
GAGTTCACAG CCACCTTCTT CCCACAGCCC ACGTTTTGTT CCGTCTGCCA TGAATTTGTC 
TGGGGTCTGA ACAAACAAGG CTACCAGTGT CAACAATGTA ATGCAGCAAT TCACAAGAAG 
TGTATCGATA AAGTAATAGC AAAGTGCACA GGATCAGCTA TCAACAGCCG AGAAACTATG 
TTCCACAAGG AGAGGTTCAA AATCGACATG CCGCACAGAT TTAAGGTCTA CAATTACAAG 
AGCCCAACCT TCTGTGAGCA CTGTGGGACC CTGCTGTGGG GCCTGGCCAG GCAAGGACTC 
AAGTGCGACG CATGTGGCAT GAATGTGCAT CACAGATGCC AGACAAAGGT GGCCAACCTT 
TGTGGTATAA ACCAGAAGCT GATGGCTGAA GCACTGGCAA TGATCGAGAG CACTCAGCAG 
GCTCGCTGCT TAAGAGATTC TGAGCACATC TTCAGAGAAG GTCCAGTTGA AATTGGTCTC 
CCATGCTCCA TAAAAGGTGA AGCAAAGCCG CCGTACGTAC CAGGACCAGG GAAAAAAGAG 
CCTCAGGGGA TCTCCTGGGA GTCTCCCTTG GAAGAGATGG AGAAAGTATG CCATCGTCCA 
GAACCTGAGC CGAACAAAGA AAGAGGTTCT CTACGTATGA AACTAAAAAT CGAGGATTTC 
ACCTTGCACA AAATGCTGGG GAAAGGAAGT TTTGGCAAGG TCTTCCTAGC AGAGTTCAAG 
AAAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTTTT GATGGATGAT 
GACGTGGAGT GCACAATGGT AGAGAAGAGG GTCCTCTCCT TGGCCTGGGA GCATCCGTTC 
TTAACACATA TGTTCTGCAC GTTCCAGACC AAGGAGAACC TCTTTTTTGT GATGGAGTAT 
CTCAACGGAG GAGACTTAAT GTACCACATC CAGAGCTGCC ACAAGTTCGA CCTCTGCAGG 
GCCACGTTCT ACGCTGCCGA AATCATCCTC GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT GGATAACATC CTCTTAGACA AAGATGGACA CATCAAAATC 
GCAGACTTCG GGATGTGCAA GGAGAACATG CTGGGAGACG CCAGGACCAA CACCTTCTGC 
GGGACCCCCG ACTACATCGC GCCGGAGATC CTCCTGGGTC AGAAGTACAA CCACTCTGTG 
GACTGGTGGT CCTTCGGTGT CCTCCTCTAC GAGATGCTAG TAGGCCAGTC CCCTTTCCAC 
GGGCAGGATG AAGAGGAGCT TTTCCACTCC ATCCGCATGG ACAATCCCTT TTACCCGCGA 
TGGCTCGAGA AGGAGGCGAA GGACCTTTTA GTGAAGCTCT TCGTAAGAGA GCCTGAGAAG 
CGGCTGGGGG TGAGGGGAGA CATCCGTCAG CACCCCTTGT TTCGGGAGAT CAATTGGGAA 
GAGCTGGAAA GGAAGGAGAT CGACCCGCCA TTCAGGCCTA AAGTGAAATC ACCATATGAC 
TGCAGCAATT TCGACAAGGA ATTCCTGAAT GAGAAGCCCC GGCTGTCCTT CGCAGACAGG 
GCGCTGATCA ACAGCATGGA CCAGAACATG TTCCGGAACT TTTCCTTCAT GAACCCAGGG 
ATGGAGAAGC TGATATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PRKCQ cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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