AACS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AACS
Entrez Gene ID	505842
Full Name	acetoacetyl-CoA synthetase
General protein information	Preferred Names acetoacetyl-CoA synthetase Names acetoacetyl-CoA synthetase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010813886.3	XP_010812188.1	acetoacetyl-CoA synthetase isoform X2
XM_010813885.2	XP_010812187.1	acetoacetyl-CoA synthetase isoform X1
XM_010813885.3	XP_010812187.1	acetoacetyl-CoA synthetase isoform X1
XM_024977079.1	XP_024832847.1	acetoacetyl-CoA synthetase isoform X3
NM_001163929.1	NP_001157401.1	acetoacetyl-CoA synthetase
XM_010813886.2	XP_010812188.1	acetoacetyl-CoA synthetase isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aacs	NP_084486.1
Neurospora crassa	NCU00446	XP_956765.1
Danio rerio (zebrafish)	aacs	NP_957303.1
Caenorhabditis elegans (roundworm)	sur-5	NP_509229.1
Magnaporthe oryzae (rice blast fungus)	MGG_05196	XP_003712739.1
Canis lupus familiaris (dog)	AACS	XP_543365.3
Xenopus tropicalis (tropical clawed frog)	aacs	NP_001039244.1
Homo sapiens (human)	AACS	NP_076417.2
	AACS	XP_001103579.1
Rattus norvegicus (Norway rat)	Aacs	NP_075592.1
Pan troglodytes (chimpanzee)	AACS	XP_001135792.2
Bos taurus (cattle)	AACS	NP_001157401.1
Gallus gallus (chicken)	AACS	NP_001006184.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AACS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AACS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa46366	XM_010813885.3 Latest version!	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa46367	XM_010813886.3 Latest version!	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa36202	NM_001163929.1 Latest version!	Bos taurus acetoacetyl-CoA synthetase (AACS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa224184	XM_024977079.1 Latest version!	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa46366	XM_010813885.2	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa46367	XM_010813886.2	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AACS ( XM_010813885.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812187.1 Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa46366
Clone ID Related Accession (Same CDS sequence)	XM_010813885.2 , XM_010813885.3
Accession Version	XM_010813885.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acetoacetyl-CoA synthetase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAAGCCTTCG AGGAGGTGCT GGACAGCCTC TGTGTCCCCC AGTACAACAA GGACGGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCT TCCGGCCACA CCTTCCGGCC TGACCTGGTG 
AAGCGGATCC GTGAGGCCAT CCGCCTCGGC CTGTCTGCCC GGCACGTGCC CAGCCTCATT 
CTGGAGACGC AGGGCATCCC GGGGTCTGGA CAGAACTGGC CACATCTCCG AAGAATGCCT 
ATTTTAGTTT GCTGTCGCTG TTGGAACAGA TTACCACACA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812187.1
CDS	163..2127
Translation	MSKEPRAGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALENYDDLYHWSVESYSDFWAEFWKFSGIVFSRMYDEVVDTSKGIADVPEWFKGSRLNYAENLLQHKENDRVALYVAREGKEEIVKVTFEELRQQVALFAAAMRKMGVRRGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHSHMDKLQRVVKGLPDLKKVVLIPYISSREKIDISKIPNSVFLEDFLATGQGDQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHILHGNMTSSDIILYYTTVGWMMWNWVVSALATGAAVVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMRPVETHSLQTLHTILSTGSPLKAQSYDYVYRCIKSSVLLGSISGGTDIVSCFMGQNPSIPVYKGEIQARNLGMAVEAWNEQGKAVWGESGELVCTKPLPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFEEVLDSLCVPQYNKDGEERVILFLKMASGHTFRPDLVKRIREAIRLGLSARHVPSLILETQGIPGSGQNWPHLRRMPILVCCRCWNRLPHT

Target ORF information:

RefSeq Version	XM_010813885.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813885.2

ORF Insert Sequence:

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAAGCCTTCG AGGAGGTGCT GGACAGCCTC TGTGTCCCCC AGTACAACAA GGACGGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCT TCCGGCCACA CCTTCCGGCC TGACCTGGTG 
AAGCGGATCC GTGAGGCCAT CCGCCTCGGC CTGTCTGCCC GGCACGTGCC CAGCCTCATT 
CTGGAGACGC AGGGCATCCC GGGGTCTGGA CAGAACTGGC CACATCTCCG AAGAATGCCT 
ATTTTAGTTT GCTGTCGCTG TTGGAACAGA TTACCACACA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( XM_010813885.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812187.1 Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa46366
Clone ID Related Accession (Same CDS sequence)	XM_010813885.2 , XM_010813885.3
Accession Version	XM_010813885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acetoacetyl-CoA synthetase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAAGCCTTCG AGGAGGTGCT GGACAGCCTC TGTGTCCCCC AGTACAACAA GGACGGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCT TCCGGCCACA CCTTCCGGCC TGACCTGGTG 
AAGCGGATCC GTGAGGCCAT CCGCCTCGGC CTGTCTGCCC GGCACGTGCC CAGCCTCATT 
CTGGAGACGC AGGGCATCCC GGGGTCTGGA CAGAACTGGC CACATCTCCG AAGAATGCCT 
ATTTTAGTTT GCTGTCGCTG TTGGAACAGA TTACCACACA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812187.1
CDS	1..1965
Translation	MSKEPRAGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALENYDDLYHWSVESYSDFWAEFWKFSGIVFSRMYDEVVDTSKGIADVPEWFKGSRLNYAENLLQHKENDRVALYVAREGKEEIVKVTFEELRQQVALFAAAMRKMGVRRGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHSHMDKLQRVVKGLPDLKKVVLIPYISSREKIDISKIPNSVFLEDFLATGQGDQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHILHGNMTSSDIILYYTTVGWMMWNWVVSALATGAAVVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMRPVETHSLQTLHTILSTGSPLKAQSYDYVYRCIKSSVLLGSISGGTDIVSCFMGQNPSIPVYKGEIQARNLGMAVEAWNEQGKAVWGESGELVCTKPLPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFEEVLDSLCVPQYNKDGEERVILFLKMASGHTFRPDLVKRIREAIRLGLSARHVPSLILETQGIPGSGQNWPHLRRMPILVCCRCWNRLPHT

Target ORF information:

RefSeq Version	XM_010813885.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813885.3

ORF Insert Sequence:

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAAGCCTTCG AGGAGGTGCT GGACAGCCTC TGTGTCCCCC AGTACAACAA GGACGGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCT TCCGGCCACA CCTTCCGGCC TGACCTGGTG 
AAGCGGATCC GTGAGGCCAT CCGCCTCGGC CTGTCTGCCC GGCACGTGCC CAGCCTCATT 
CTGGAGACGC AGGGCATCCC GGGGTCTGGA CAGAACTGGC CACATCTCCG AAGAATGCCT 
ATTTTAGTTT GCTGTCGCTG TTGGAACAGA TTACCACACA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( XM_010813886.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812188.1 Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa46367
Clone ID Related Accession (Same CDS sequence)	XM_010813886.3 , XM_010813886.2
Accession Version	XM_010813886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acetoacetyl-CoA synthetase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGTTGG CTGGATGATG TGGAACTGGG TGGTGTCTGC TCTTGCCACG 
GGGGCCGCTG TGGTCCTGTA TGACGGCTCC CCGCTGGTTC CGACACCCAA CGTGCTCTGG 
GATCTGGTCG ACAGGATCGG CATCACCATC CTTGGAACGG GTGCTAAGTG GCTCTCTGTG 
CTTGAAGAGA AAGACATGAG GCCAGTGGAA ACCCACAGTC TCCAGACACT GCACACGATC 
CTGTCCACTG GCTCCCCACT GAAAGCCCAG AGCTACGACT ACGTCTACAG GTGCATCAAG 
AGCAGCGTCC TGCTGGGCTC CATCTCAGGT GGCACCGACA TCGTCTCCTG CTTCATGGGC 
CAGAATCCAT CCATTCCTGT GTACAAAGGG GAGATTCAGG CTCGGAACCT GGGCATGGCT 
GTGGAGGCAT GGAATGAGCA AGGAAAGGCT GTGTGGGGCG AGAGTGGTGA GCTGGTGTGC 
ACCAAGCCAC TCCCCTGCCA GCCCACGCAC TTCTGGAATG ACGAGAATGG CAGCAAGTAC 
CGGAAGGCGT ACTTCTCCAA GTTCCCAGGT GTCTGGGCAC ATGGTGACTA CTGCAGGATA 
AATCCCAAGA CCGGGGGAAT CGTCATGCTG GGCCGAAGTG ATGGCACGCT CAACCCCAAT 
GGAGTGCGAT TCGGCAGCTC GGAAATCTAT AACATTGTGG AAGCCTTCGA GGAGGTGCTG 
GACAGCCTCT GTGTCCCCCA GTACAACAAG GACGGGGAGG AGAGGGTGAT CCTCTTCCTG 
AAGATGGCTT CCGGCCACAC CTTCCGGCCT GACCTGGTGA AGCGGATCCG TGAGGCCATC 
CGCCTCGGCC TGTCTGCCCG GCACGTGCCC AGCCTCATTC TGGAGACGCA GGGCATCCCG 
TACACCCTCA ACGGCAAGAA GGTGGAAGTG GCTGTGAAGC AGATCATCGC TGGGAAGACG 
GTGGAGCACC GAGGGGCCTT CTCGAATCCC GAGACCCTGG ATTTGTACCG GGACATCCCT 
CAGCTGCAGG ACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812188.1
CDS	153..2090
Translation	MSKEPRAGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALENYDDLYHWSVESYSDFWAEFWKFSGIVFSRMYDEVVDTSKGIADVPEWFKGSRLNYAENLLQHKENDRVALYVAREGKEEIVKVTFEELRQQVALFAAAMRKMGVRRGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHSHMDKLQRVVKGLPDLKKVVLIPYISSREKIDISKIPNSVFLEDFLATGQGDQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGVGWMMWNWVVSALATGAAVVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMRPVETHSLQTLHTILSTGSPLKAQSYDYVYRCIKSSVLLGSISGGTDIVSCFMGQNPSIPVYKGEIQARNLGMAVEAWNEQGKAVWGESGELVCTKPLPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFEEVLDSLCVPQYNKDGEERVILFLKMASGHTFRPDLVKRIREAIRLGLSARHVPSLILETQGIPYTLNGKKVEVAVKQIIAGKTVEHRGAFSNPETLDLYRDIPQLQDF

Target ORF information:

RefSeq Version	XM_010813886.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813886.3

ORF Insert Sequence:

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGTTGG CTGGATGATG TGGAACTGGG TGGTGTCTGC TCTTGCCACG 
GGGGCCGCTG TGGTCCTGTA TGACGGCTCC CCGCTGGTTC CGACACCCAA CGTGCTCTGG 
GATCTGGTCG ACAGGATCGG CATCACCATC CTTGGAACGG GTGCTAAGTG GCTCTCTGTG 
CTTGAAGAGA AAGACATGAG GCCAGTGGAA ACCCACAGTC TCCAGACACT GCACACGATC 
CTGTCCACTG GCTCCCCACT GAAAGCCCAG AGCTACGACT ACGTCTACAG GTGCATCAAG 
AGCAGCGTCC TGCTGGGCTC CATCTCAGGT GGCACCGACA TCGTCTCCTG CTTCATGGGC 
CAGAATCCAT CCATTCCTGT GTACAAAGGG GAGATTCAGG CTCGGAACCT GGGCATGGCT 
GTGGAGGCAT GGAATGAGCA AGGAAAGGCT GTGTGGGGCG AGAGTGGTGA GCTGGTGTGC 
ACCAAGCCAC TCCCCTGCCA GCCCACGCAC TTCTGGAATG ACGAGAATGG CAGCAAGTAC 
CGGAAGGCGT ACTTCTCCAA GTTCCCAGGT GTCTGGGCAC ATGGTGACTA CTGCAGGATA 
AATCCCAAGA CCGGGGGAAT CGTCATGCTG GGCCGAAGTG ATGGCACGCT CAACCCCAAT 
GGAGTGCGAT TCGGCAGCTC GGAAATCTAT AACATTGTGG AAGCCTTCGA GGAGGTGCTG 
GACAGCCTCT GTGTCCCCCA GTACAACAAG GACGGGGAGG AGAGGGTGAT CCTCTTCCTG 
AAGATGGCTT CCGGCCACAC CTTCCGGCCT GACCTGGTGA AGCGGATCCG TGAGGCCATC 
CGCCTCGGCC TGTCTGCCCG GCACGTGCCC AGCCTCATTC TGGAGACGCA GGGCATCCCG 
TACACCCTCA ACGGCAAGAA GGTGGAAGTG GCTGTGAAGC AGATCATCGC TGGGAAGACG 
GTGGAGCACC GAGGGGCCTT CTCGAATCCC GAGACCCTGG ATTTGTACCG GGACATCCCT 
CAGCTGCAGG ACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( XM_010813886.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812188.1 Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X2, mRNA.

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CloneID	OBa46367
Clone ID Related Accession (Same CDS sequence)	XM_010813886.3 , XM_010813886.2
Accession Version	XM_010813886.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acetoacetyl-CoA synthetase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGTTGG CTGGATGATG TGGAACTGGG TGGTGTCTGC TCTTGCCACG 
GGGGCCGCTG TGGTCCTGTA TGACGGCTCC CCGCTGGTTC CGACACCCAA CGTGCTCTGG 
GATCTGGTCG ACAGGATCGG CATCACCATC CTTGGAACGG GTGCTAAGTG GCTCTCTGTG 
CTTGAAGAGA AAGACATGAG GCCAGTGGAA ACCCACAGTC TCCAGACACT GCACACGATC 
CTGTCCACTG GCTCCCCACT GAAAGCCCAG AGCTACGACT ACGTCTACAG GTGCATCAAG 
AGCAGCGTCC TGCTGGGCTC CATCTCAGGT GGCACCGACA TCGTCTCCTG CTTCATGGGC 
CAGAATCCAT CCATTCCTGT GTACAAAGGG GAGATTCAGG CTCGGAACCT GGGCATGGCT 
GTGGAGGCAT GGAATGAGCA AGGAAAGGCT GTGTGGGGCG AGAGTGGTGA GCTGGTGTGC 
ACCAAGCCAC TCCCCTGCCA GCCCACGCAC TTCTGGAATG ACGAGAATGG CAGCAAGTAC 
CGGAAGGCGT ACTTCTCCAA GTTCCCAGGT GTCTGGGCAC ATGGTGACTA CTGCAGGATA 
AATCCCAAGA CCGGGGGAAT CGTCATGCTG GGCCGAAGTG ATGGCACGCT CAACCCCAAT 
GGAGTGCGAT TCGGCAGCTC GGAAATCTAT AACATTGTGG AAGCCTTCGA GGAGGTGCTG 
GACAGCCTCT GTGTCCCCCA GTACAACAAG GACGGGGAGG AGAGGGTGAT CCTCTTCCTG 
AAGATGGCTT CCGGCCACAC CTTCCGGCCT GACCTGGTGA AGCGGATCCG TGAGGCCATC 
CGCCTCGGCC TGTCTGCCCG GCACGTGCCC AGCCTCATTC TGGAGACGCA GGGCATCCCG 
TACACCCTCA ACGGCAAGAA GGTGGAAGTG GCTGTGAAGC AGATCATCGC TGGGAAGACG 
GTGGAGCACC GAGGGGCCTT CTCGAATCCC GAGACCCTGG ATTTGTACCG GGACATCCCT 
CAGCTGCAGG ACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812188.1
CDS	162..2099
Translation	MSKEPRAGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALENYDDLYHWSVESYSDFWAEFWKFSGIVFSRMYDEVVDTSKGIADVPEWFKGSRLNYAENLLQHKENDRVALYVAREGKEEIVKVTFEELRQQVALFAAAMRKMGVRRGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHSHMDKLQRVVKGLPDLKKVVLIPYISSREKIDISKIPNSVFLEDFLATGQGDQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGVGWMMWNWVVSALATGAAVVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMRPVETHSLQTLHTILSTGSPLKAQSYDYVYRCIKSSVLLGSISGGTDIVSCFMGQNPSIPVYKGEIQARNLGMAVEAWNEQGKAVWGESGELVCTKPLPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFEEVLDSLCVPQYNKDGEERVILFLKMASGHTFRPDLVKRIREAIRLGLSARHVPSLILETQGIPYTLNGKKVEVAVKQIIAGKTVEHRGAFSNPETLDLYRDIPQLQDF

Target ORF information:

RefSeq Version	XM_010813886.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813886.2

ORF Insert Sequence:

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGTTGG CTGGATGATG TGGAACTGGG TGGTGTCTGC TCTTGCCACG 
GGGGCCGCTG TGGTCCTGTA TGACGGCTCC CCGCTGGTTC CGACACCCAA CGTGCTCTGG 
GATCTGGTCG ACAGGATCGG CATCACCATC CTTGGAACGG GTGCTAAGTG GCTCTCTGTG 
CTTGAAGAGA AAGACATGAG GCCAGTGGAA ACCCACAGTC TCCAGACACT GCACACGATC 
CTGTCCACTG GCTCCCCACT GAAAGCCCAG AGCTACGACT ACGTCTACAG GTGCATCAAG 
AGCAGCGTCC TGCTGGGCTC CATCTCAGGT GGCACCGACA TCGTCTCCTG CTTCATGGGC 
CAGAATCCAT CCATTCCTGT GTACAAAGGG GAGATTCAGG CTCGGAACCT GGGCATGGCT 
GTGGAGGCAT GGAATGAGCA AGGAAAGGCT GTGTGGGGCG AGAGTGGTGA GCTGGTGTGC 
ACCAAGCCAC TCCCCTGCCA GCCCACGCAC TTCTGGAATG ACGAGAATGG CAGCAAGTAC 
CGGAAGGCGT ACTTCTCCAA GTTCCCAGGT GTCTGGGCAC ATGGTGACTA CTGCAGGATA 
AATCCCAAGA CCGGGGGAAT CGTCATGCTG GGCCGAAGTG ATGGCACGCT CAACCCCAAT 
GGAGTGCGAT TCGGCAGCTC GGAAATCTAT AACATTGTGG AAGCCTTCGA GGAGGTGCTG 
GACAGCCTCT GTGTCCCCCA GTACAACAAG GACGGGGAGG AGAGGGTGAT CCTCTTCCTG 
AAGATGGCTT CCGGCCACAC CTTCCGGCCT GACCTGGTGA AGCGGATCCG TGAGGCCATC 
CGCCTCGGCC TGTCTGCCCG GCACGTGCCC AGCCTCATTC TGGAGACGCA GGGCATCCCG 
TACACCCTCA ACGGCAAGAA GGTGGAAGTG GCTGTGAAGC AGATCATCGC TGGGAAGACG 
GTGGAGCACC GAGGGGCCTT CTCGAATCCC GAGACCCTGG ATTTGTACCG GGACATCCCT 
CAGCTGCAGG ACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_001163929.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001157401.1 Bos taurus acetoacetyl-CoA synthetase (AACS), mRNA.

Price & Availability↑

CloneID	OBa36202
Clone ID Related Accession (Same CDS sequence)	NM_001163929.1
Accession Version	NM_001163929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	acetoacetyl-CoA synthetase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Aug 8, 2009 this sequence version replaced XM_582199.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.47504.1, SRR5571259.32139.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAAGCCTTCG AGGAGGTGCT GGACAGCCTC TGTGTCCCCC AGTACAACAA GGACGGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCT TCCGGCCACA CCTTCCGGCC TGACCTGGTG 
AAGCGGATCC GTGAGGCCAT CCGCCTCGGC CTGTCTGCCC GGCACGTGCC CAGCCTCATT 
CTGGAGACGC AGGGCATCCC GTACACCCTC AACGGCAAGA AGGTGGAAGT GGCTGTGAAG 
CAGATCATCG CTGGGAAGAC GGTGGAGCAC CGAGGGGCCT TCTCGAATCC CGAGACCCTG 
GATTTGTACC GGGACATCCC TCAGCTGCAG GACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157401.1
CDS	1..2019
Translation	MSKEPRAGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALENYDDLYHWSVESYSDFWAEFWKFSGIVFSRMYDEVVDTSKGIADVPEWFKGSRLNYAENLLQHKENDRVALYVAREGKEEIVKVTFEELRQQVALFAAAMRKMGVRRGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHSHMDKLQRVVKGLPDLKKVVLIPYISSREKIDISKIPNSVFLEDFLATGQGDQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHILHGNMTSSDIILYYTTVGWMMWNWVVSALATGAAVVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMRPVETHSLQTLHTILSTGSPLKAQSYDYVYRCIKSSVLLGSISGGTDIVSCFMGQNPSIPVYKGEIQARNLGMAVEAWNEQGKAVWGESGELVCTKPLPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFEEVLDSLCVPQYNKDGEERVILFLKMASGHTFRPDLVKRIREAIRLGLSARHVPSLILETQGIPYTLNGKKVEVAVKQIIAGKTVEHRGAFSNPETLDLYRDIPQLQDF

Target ORF information:

RefSeq Version	NM_001163929.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acetoacetyl-CoA synthetase (AACS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163929.1

ORF Insert Sequence:

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAAGCCTTCG AGGAGGTGCT GGACAGCCTC TGTGTCCCCC AGTACAACAA GGACGGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCT TCCGGCCACA CCTTCCGGCC TGACCTGGTG 
AAGCGGATCC GTGAGGCCAT CCGCCTCGGC CTGTCTGCCC GGCACGTGCC CAGCCTCATT 
CTGGAGACGC AGGGCATCCC GTACACCCTC AACGGCAAGA AGGTGGAAGT GGCTGTGAAG 
CAGATCATCG CTGGGAAGAC GGTGGAGCAC CGAGGGGCCT TCTCGAATCC CGAGACCCTG 
GATTTGTACC GGGACATCCC TCAGCTGCAG GACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( XM_024977079.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832847.1 Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X3, mRNA.

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CloneID	OBa224184
Clone ID Related Accession (Same CDS sequence)	XM_024977079.1
Accession Version	XM_024977079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acetoacetyl-CoA synthetase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTA 
CACCCTCAAC GGCAAGAAGG TGGAAGTGGC TGTGAAGCAG ATCATCGCTG GGAAGACGGT 
GGAGCACCGA GGGGCCTTCT CGAATCCCGA GACCCTGGAT TTGTACCGGG ACATCCCTCA 
GCTGCAGGAC TTTTGAGCCG AGCTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832847.1
CDS	154..1983
Translation	MSKEPRAGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALENYDDLYHWSVESYSDFWAEFWKFSGIVFSRMYDEVVDTSKGIADVPEWFKGSRLNYAENLLQHKENDRVALYVAREGKEEIVKVTFEELRQQVALFAAAMRKMGVRRGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHSHMDKLQRVVKGLPDLKKVVLIPYISSREKIDISKIPNSVFLEDFLATGQGDQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHILHGNMTSSDIILYYTTVGWMMWNWVVSALATGAAVVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMRPVETHSLQTLHTILSTGSPLKAQSYDYVYRCIKSSVLLGSISGGTDIVSCFMGQNPSIPVYKGEIQARNLGMAVEAWNEQGKAVWGESGELVCTKPLPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVHPQRQEGGSGCEADHRWEDGGAPRGLLESRDPGFVPGHPSAAGLLSRAG

Target ORF information:

RefSeq Version	XM_024977079.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acetoacetyl-CoA synthetase (AACS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977079.1

ORF Insert Sequence:

ATGTCCAAGG AGCCACGGGC CGGGCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CAGCAGTGGG CGCCGCCTGC 
GGCCTGGCAC TGGAAAATTA TGATGACCTG TATCATTGGT CAGTTGAGTC GTATTCAGAC 
TTCTGGGCAG AATTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGCATGTA TGATGAGGTC 
GTGGACACAT CCAAAGGGAT CGCCGACGTG CCTGAGTGGT TCAAAGGCAG TCGCCTCAAC 
TACGCAGAAA ACCTCCTGCA ACACAAAGAA AACGACCGAG TGGCCCTTTA TGTCGCACGG 
GAAGGCAAGG AGGAGATCGT GAAGGTGACT TTTGAAGAGT TGCGGCAACA GGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA GATGGGCGTG AGGAGAGGAG ATCGGGTCGT TGGCTACTTG 
CCCAACAGCG CGCATGCTGT GGAGGCCATG CTGGCGGCTG CGAGCATTGG GGCCATCTGG 
AGTTCCACGT CCCCAGACTT TGGCGTGAAT GGCGTTCTGG ACCGGTTTTC TCAGATCCAG 
CCGAAGCTCA TCTTCTCTGT GGAGGCTGTT GTGTACAATG GCAAAGAGCA CAGCCATATG 
GACAAGCTGC AGCGGGTCGT TAAGGGACTA CCAGACCTAA AAAAAGTGGT ACTGATTCCT 
TACATCTCCT CCAGAGAGAA GATAGACATC TCCAAGATTC CAAATAGCGT GTTCCTGGAG 
GATTTCCTGG CCACCGGGCA AGGCGACCAA GCCCCCCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTCTTCAT TATGTTTTCG TCAGGCACGA CAGGAGCACC CAAGTGCATG 
GTGCACTCGG CCGGGGGGAC GCTCATCCAG CACCTGAAGG AACACATTCT TCATGGCAAC 
ATGACCAGCA GTGACATCAT TTTGTATTAC ACCACGGTTG GCTGGATGAT GTGGAACTGG 
GTGGTGTCTG CTCTTGCCAC GGGGGCCGCT GTGGTCCTGT ATGACGGCTC CCCGCTGGTT 
CCGACACCCA ACGTGCTCTG GGATCTGGTC GACAGGATCG GCATCACCAT CCTTGGAACG 
GGTGCTAAGT GGCTCTCTGT GCTTGAAGAG AAAGACATGA GGCCAGTGGA AACCCACAGT 
CTCCAGACAC TGCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAC 
TACGTCTACA GGTGCATCAA GAGCAGCGTC CTGCTGGGCT CCATCTCAGG TGGCACCGAC 
ATCGTCTCCT GCTTCATGGG CCAGAATCCA TCCATTCCTG TGTACAAAGG GGAGATTCAG 
GCTCGGAACC TGGGCATGGC TGTGGAGGCA TGGAATGAGC AAGGAAAGGC TGTGTGGGGC 
GAGAGTGGTG AGCTGGTGTG CACCAAGCCA CTCCCCTGCC AGCCCACGCA CTTCTGGAAT 
GACGAGAATG GCAGCAAGTA CCGGAAGGCG TACTTCTCCA AGTTCCCAGG TGTCTGGGCA 
CATGGTGACT ACTGCAGGAT AAATCCCAAG ACCGGGGGAA TCGTCATGCT GGGCCGAAGT 
GATGGCACGC TCAACCCCAA TGGAGTGCGA TTCGGCAGCT CGGAAATCTA TAACATTGTA 
CACCCTCAAC GGCAAGAAGG TGGAAGTGGC TGTGAAGCAG ATCATCGCTG GGAAGACGGT 
GGAGCACCGA GGGGCCTTCT CGAATCCCGA GACCCTGGAT TTGTACCGGG ACATCCCTCA 
GCTGCAGGAC TTTTGAGCCG AGCTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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AACS cDNA ORF clone, Bos taurus(cattle)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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