FPGS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FPGS
Entrez Gene ID	505809
Full Name	folylpolyglutamate synthase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024998398.1	XP_024854166.1	folylpolyglutamate synthase, mitochondrial isoform X4
XM_024998395.1	XP_024854163.1	folylpolyglutamate synthase, mitochondrial isoform X2
NM_001024480.2	NP_001019651.2	folylpolyglutamate synthase, mitochondrial
XM_024998394.1	XP_024854162.1	folylpolyglutamate synthase, mitochondrial isoform X1
XM_024998396.1	XP_024854164.1	folylpolyglutamate synthase, mitochondrial isoform X2
XM_024998397.1	XP_024854165.1	folylpolyglutamate synthase, mitochondrial isoform X3
XM_024998399.1	XP_024854167.1	folylpolyglutamate synthase, mitochondrial isoform X5

Pathways from BioSystems

Homologies

	FPGS	XP_001094027.1
Canis lupus familiaris (dog)	FPGS	XP_003639360.1
Bos taurus (cattle)	FPGS	NP_001019651.2
Mus musculus (house mouse)	Fpgs	NP_034366.2
Magnaporthe oryzae (rice blast fungus)	MGG_11267	XP_003714094.1
Arabidopsis thaliana (thale cress)	DFB	NP_196217.2
Xenopus tropicalis (tropical clawed frog)	fpgs	NP_001096413.1
Homo sapiens (human)	FPGS	NP_004948.4
Gallus gallus (chicken)	FPGS	XP_003642325.2
Saccharomyces cerevisiae (baker's yeast)	MET7	NP_014884.1
Kluyveromyces lactis	KLLA0F11297g	XP_455594.1
Rattus norvegicus (Norway rat)	Fpgs	NP_001139597.2
Caenorhabditis elegans (roundworm)	F25B5.6	NP_498292.1
Neurospora crassa	NCU00892	XP_964808.2
Eremothecium gossypii	AGOS_ACR134W	NP_983536.2
Oryza sativa (rice)	Os10g0503200	NP_001064996.1
Pan troglodytes (chimpanzee)	FPGS	XP_003312383.1
Danio rerio (zebrafish)	fpgs	NP_998602.1
Drosophila melanogaster (fruit fly)	CG2543	NP_572814.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004679	XP_001688580.1
Schizosaccharomyces pombe (fission yeast)	SPBC1709.17	NP_595450.1
Oryza sativa (rice)	Os03g0111100	NP_001048721.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FPGS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FPGS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44317	NM_001024480.2 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa220188	XM_024998398.1 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa220189	XM_024998399.1 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa220185	XM_024998394.1 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa220186	XM_024998396.1 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa220186	XM_024998395.1 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa220187	XM_024998397.1 Latest version!	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FPGS ( NM_001024480.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019651.2 Bos taurus folylpolyglutamate synthase (FPGS), mRNA.

Price & Availability↑

CloneID	OBa44317
Clone ID Related Accession (Same CDS sequence)	NM_001024480.2
Accession Version	NM_001024480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC146114.1, BE666530.1 and CO888657.1. On Oct 17, 2009 this sequence version replaced NM_001024480.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCTCGGG CACGCTGCCA CGCTCTCTTC CTGGCAGCGG TTTCTCCGCG TGGTGCAACA 
ACCAGGGTCG CAGTGCGGCG AGGGTTGAGC GCGTGGCCTG TGCTGCAGGA GCCAGACATG 
GAGTATCAGG ATGCCGTGCG TACACTCAAC ACCCTGCAGA CCAATGCTAA CTACTTGGAG 
ATGGTGAAGC GTAAGCGGGG TGACCCCCAG ACACAGCTGG AAGCCATGAA GCTGTACTTG 
GCACGGAGTG GGCTGCAGGT GGAGGACTTG GACCAGCTGA ACATTATCCA CATCACTGGA 
ACCAAGGGGA AGGGCTCCAC GTGTGCCTTC ACTGAACGTA TCCTCCGAAG CTATGGCCTG 
AAGACGGGGT TCTTTAGCTC TCCCCACCTG GTGCAGGTGC GTGAGCGGAT CCGCATCAAT 
GGGCAGCCCA TAAGCTCCGA GCTCTTCACC AAGCACTTCT GGCGCCTCTA CCACCGGCTG 
GAGGAGACCA AGGACGACAG CAGCTGTGTC TCCATGCCCG GCTACTTCCG CTTCCTCACG 
CTCATGGCCT TCCACGTCTT CCTCCAAGAG AAGGTAGACC TGGCAGTGTT GGAAGTAGGC 
ATTGGCGGGG CTTACGACTG CACCAACATC ATCAGGAAGC CTGTGGTGTG TGGGATCACC 
TCTCTTGGCA TCGACCACAC TGGCCTTCTG GGGGACACGA TGGAGAAGAT CGCATGGCAG 
AAAGGGGGCA TTTTTAAGAG TGGCGTCCCT GCCTTCACCG TGCTCCAGCC TGACGAGCCG 
CTGGCGGTGC TAAGGGACAG GGCTCAGCAG ATCTCATGTC CCCTCTACCT GTGCCCACCA 
CTACAAGCCC TGGAGGAAGG GGGGCCACCA CTGACCCTGG GCCTGGAGGG AGAGCACCAG 
CGGTCCAATG CTGCCCTGGC CTTGCAGGTG GCCCGCTGCT GGCTGCAGCA GAAAGGTTAC 
CAGGATGCTG GGGAGCTGAA GGCATCCAGG CCCAGCCTCC CGGGGCAGCT GCCCCTGGCA 
CCTGTGTTCC AGCCCACGCC CCGCATGCAG CAGGGGCTTC GGCACACGGA ATGGCCAGGT 
CGGACGCAGT TGCTGCGGCG CGGGCCTCTT ACCTGGTACC TGGACGGCGC GCACACCACC 
AGCAGCATGC AAGCCTGTGT GCGCTGGTTC CGCCAGGCGC TGCACCGCTG CGAGAGGCCG 
GACAGCGGTT CCGAGGTGCG CGTCTTGCTC TTCAACTCCA CTGGGGACCG GGATTCCGCG 
GCCCTGCTGA AGCTGCTGCA GCCCTGCCAG TTTGACTATG CCATTTTCTG CCCTAACCTG 
GCAGAGATGG CATCAACAGA CAATGCAGAC CAACAGAACT TCACAGTGAC ACTGGACCAG 
GTGCTGCTCC GCTGCCTCGC CCACCAACAG CACTGGAGTC GCCTGCATGA GGAGCAGGTC 
AGCCCGGACC CCTGGAGCAC CCCCGGCCAG GAGCAGGATG GGCCCGCATC TCTGCTGCTG 
GCGCCTCACC CACCCCACAC CCACAGTGCC AGCTCCCTCG TCTTCAGCTG CATCTCCCAT 
GCCTTGCAGT GGATCACCCA AGGCCGGGAC CCTCTCTTTC AGCCGCCCAG TCCCCCACGG 
GGCCTCCTCG CCCACCCTGT GGCAGACAGT GGGGCCACGG TACTCCAAGA GGCTGCTGAC 
ATTCACGTGC TGGTCACAGG CAGCCTGCAC CTGGTGGGCG GTGTCCTGAA GCTGCTGGAG 
CCCTCCCTGT CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019651.2
CDS	47..1804
Translation	MSRARCHALFLAAVSPRGATTRVAVRRGLSAWPVLQEPDMEYQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAADIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	NM_001024480.2
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024480.2

ORF Insert Sequence:

ATGTCTCGGG CACGCTGCCA CGCTCTCTTC CTGGCAGCGG TTTCTCCGCG TGGTGCAACA 
ACCAGGGTCG CAGTGCGGCG AGGGTTGAGC GCGTGGCCTG TGCTGCAGGA GCCAGACATG 
GAGTATCAGG ATGCCGTGCG TACACTCAAC ACCCTGCAGA CCAATGCTAA CTACTTGGAG 
ATGGTGAAGC GTAAGCGGGG TGACCCCCAG ACACAGCTGG AAGCCATGAA GCTGTACTTG 
GCACGGAGTG GGCTGCAGGT GGAGGACTTG GACCAGCTGA ACATTATCCA CATCACTGGA 
ACCAAGGGGA AGGGCTCCAC GTGTGCCTTC ACTGAACGTA TCCTCCGAAG CTATGGCCTG 
AAGACGGGGT TCTTTAGCTC TCCCCACCTG GTGCAGGTGC GTGAGCGGAT CCGCATCAAT 
GGGCAGCCCA TAAGCTCCGA GCTCTTCACC AAGCACTTCT GGCGCCTCTA CCACCGGCTG 
GAGGAGACCA AGGACGACAG CAGCTGTGTC TCCATGCCCG GCTACTTCCG CTTCCTCACG 
CTCATGGCCT TCCACGTCTT CCTCCAAGAG AAGGTAGACC TGGCAGTGTT GGAAGTAGGC 
ATTGGCGGGG CTTACGACTG CACCAACATC ATCAGGAAGC CTGTGGTGTG TGGGATCACC 
TCTCTTGGCA TCGACCACAC TGGCCTTCTG GGGGACACGA TGGAGAAGAT CGCATGGCAG 
AAAGGGGGCA TTTTTAAGAG TGGCGTCCCT GCCTTCACCG TGCTCCAGCC TGACGAGCCG 
CTGGCGGTGC TAAGGGACAG GGCTCAGCAG ATCTCATGTC CCCTCTACCT GTGCCCACCA 
CTACAAGCCC TGGAGGAAGG GGGGCCACCA CTGACCCTGG GCCTGGAGGG AGAGCACCAG 
CGGTCCAATG CTGCCCTGGC CTTGCAGGTG GCCCGCTGCT GGCTGCAGCA GAAAGGTTAC 
CAGGATGCTG GGGAGCTGAA GGCATCCAGG CCCAGCCTCC CGGGGCAGCT GCCCCTGGCA 
CCTGTGTTCC AGCCCACGCC CCGCATGCAG CAGGGGCTTC GGCACACGGA ATGGCCAGGT 
CGGACGCAGT TGCTGCGGCG CGGGCCTCTT ACCTGGTACC TGGACGGCGC GCACACCACC 
AGCAGCATGC AAGCCTGTGT GCGCTGGTTC CGCCAGGCGC TGCACCGCTG CGAGAGGCCG 
GACAGCGGTT CCGAGGTGCG CGTCTTGCTC TTCAACTCCA CTGGGGACCG GGATTCCGCG 
GCCCTGCTGA AGCTGCTGCA GCCCTGCCAG TTTGACTATG CCATTTTCTG CCCTAACCTG 
GCAGAGATGG CATCAACAGA CAATGCAGAC CAACAGAACT TCACAGTGAC ACTGGACCAG 
GTGCTGCTCC GCTGCCTCGC CCACCAACAG CACTGGAGTC GCCTGCATGA GGAGCAGGTC 
AGCCCGGACC CCTGGAGCAC CCCCGGCCAG GAGCAGGATG GGCCCGCATC TCTGCTGCTG 
GCGCCTCACC CACCCCACAC CCACAGTGCC AGCTCCCTCG TCTTCAGCTG CATCTCCCAT 
GCCTTGCAGT GGATCACCCA AGGCCGGGAC CCTCTCTTTC AGCCGCCCAG TCCCCCACGG 
GGCCTCCTCG CCCACCCTGT GGCAGACAGT GGGGCCACGG TACTCCAAGA GGCTGCTGAC 
ATTCACGTGC TGGTCACAGG CAGCCTGCAC CTGGTGGGCG GTGTCCTGAA GCTGCTGGAG 
CCCTCCCTGT CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGS ( XM_024998398.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854166.1 Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa220188
Clone ID Related Accession (Same CDS sequence)	XM_024998398.1
Accession Version	XM_024998398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGG CACGCTGCCA CGCTCTCTTC CTGGCAGCGG TTTCTCCGCG TGGTGCAACA 
ACCAGGGTCG CAGTGCGGCG AGGGTTGAGC GCGTGGCCTG TGCTGCAGGA GCCAGACATG 
GAGTATCAGG TGCGTGAGCG GATCCGCATC AATGGGCAGC CCATAAGCTC CGAGCTCTTC 
ACCAAGCACT TCTGGCGCCT CTACCACCGG CTGGAGGAGA CCAAGGACGA CAGCAGCTGT 
GTCTCCATGC CCGGCTACTT CCGCTTCCTC ACGCTCATGG CCTTCCACGT CTTCCTCCAA 
GAGAAGGTAG ACCTGGCAGT GTTGGAAGTA GGCATTGGCG GGGCTTACGA CTGCACCAAC 
ATCATCAGGA AGCCTGTGGT GTGTGGGATC ACCTCTCTTG GCATCGACCA CACTGGCCTT 
CTGGGGGACA CGATGGAGAA GATCGCATGG CAGAAAGGGG GCATTTTTAA GAGTGGCGTC 
CCTGCCTTCA CCGTGCTCCA GCCTGACGAG CCGCTGGCGG TGCTAAGGGA CAGGGCTCAG 
CAGATCTCAT GTCCCCTCTA CCTGTGCCCA CCACTACAAG CCCTGGAGGA AGGGGGGCCA 
CCACTGACCC TGGGCCTGGA GGGAGAGCAC CAGCGGTCCA ATGCTGCCCT GGCCTTGCAG 
GTGGCCCGCT GCTGGCTGCA GCAGAAAGGT TACCAGGATG CTGGGGAGCT GAAGGCATCC 
AGGCCCAGCC TCCCGGGGCA GCTGCCCCTG GCACCTGTGT TCCAGCCCAC GCCCCGCATG 
CAGCAGGGGC TTCGGCACAC GGAATGGCCA GGTCGGACGC AGTTGCTGCG GCGCGGGCCT 
CTTACCTGGT ACCTGGACGG CGCGCACACC ACCAGCAGCA TGCAAGCCTG TGTGCGCTGG 
TTCCGCCAGG CGCTGCACCG CTGCGAGAGG CCGGACAGCG GTTCCGAGGT GCGCGTCTTG 
CTCTTCAACT CCACTGGGGA CCGGGATTCC GCGGCCCTGC TGAAGCTGCT GCAGCCCTGC 
CAGTTTGACT ATGCCATTTT CTGCCCTAAC CTGGCAGAGA TGGCATCAAC AGACAATGCA 
GACCAACAGA ACTTCACAGT GACACTGGAC CAGGTGCTGC TCCGCTGCCT CGCCCACCAA 
CAGCACTGGA GTCGCCTGCA TGAGGAGCAG GTCAGCCCGG ACCCCTGGAG CACCCCCGGC 
CAGGAGCAGG ATGGGCCCGC ATCTCTGCTG CTGGCGCCTC ACCCACCCCA CACCCACAGT 
GCCAGCTCCC TCGTCTTCAG CTGCATCTCC CATGCCTTGC AGTGGATCAC CCAAGGCCGG 
GACCCTCTCT TTCAGCCGCC CAGTCCCCCA CGGGGCCTCC TCGCCCACCC TGTGGCAGAC 
AGTGGGGCCA CGGTACTCCA AGAGGCTGCT GCCATTCACG TGCTGGTCAC AGGCAGCCTG 
CACCTGGTGG GCGGTGTCCT GAAGCTGCTG GAGCCCTCCC TGTCCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854166.1
CDS	374..1864
Translation	MSRARCHALFLAAVSPRGATTRVAVRRGLSAWPVLQEPDMEYQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAAAIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	XM_024998398.1
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998398.1

ORF Insert Sequence:

ATGTCTCGGG CACGCTGCCA CGCTCTCTTC CTGGCAGCGG TTTCTCCGCG TGGTGCAACA 
ACCAGGGTCG CAGTGCGGCG AGGGTTGAGC GCGTGGCCTG TGCTGCAGGA GCCAGACATG 
GAGTATCAGG TGCGTGAGCG GATCCGCATC AATGGGCAGC CCATAAGCTC CGAGCTCTTC 
ACCAAGCACT TCTGGCGCCT CTACCACCGG CTGGAGGAGA CCAAGGACGA CAGCAGCTGT 
GTCTCCATGC CCGGCTACTT CCGCTTCCTC ACGCTCATGG CCTTCCACGT CTTCCTCCAA 
GAGAAGGTAG ACCTGGCAGT GTTGGAAGTA GGCATTGGCG GGGCTTACGA CTGCACCAAC 
ATCATCAGGA AGCCTGTGGT GTGTGGGATC ACCTCTCTTG GCATCGACCA CACTGGCCTT 
CTGGGGGACA CGATGGAGAA GATCGCATGG CAGAAAGGGG GCATTTTTAA GAGTGGCGTC 
CCTGCCTTCA CCGTGCTCCA GCCTGACGAG CCGCTGGCGG TGCTAAGGGA CAGGGCTCAG 
CAGATCTCAT GTCCCCTCTA CCTGTGCCCA CCACTACAAG CCCTGGAGGA AGGGGGGCCA 
CCACTGACCC TGGGCCTGGA GGGAGAGCAC CAGCGGTCCA ATGCTGCCCT GGCCTTGCAG 
GTGGCCCGCT GCTGGCTGCA GCAGAAAGGT TACCAGGATG CTGGGGAGCT GAAGGCATCC 
AGGCCCAGCC TCCCGGGGCA GCTGCCCCTG GCACCTGTGT TCCAGCCCAC GCCCCGCATG 
CAGCAGGGGC TTCGGCACAC GGAATGGCCA GGTCGGACGC AGTTGCTGCG GCGCGGGCCT 
CTTACCTGGT ACCTGGACGG CGCGCACACC ACCAGCAGCA TGCAAGCCTG TGTGCGCTGG 
TTCCGCCAGG CGCTGCACCG CTGCGAGAGG CCGGACAGCG GTTCCGAGGT GCGCGTCTTG 
CTCTTCAACT CCACTGGGGA CCGGGATTCC GCGGCCCTGC TGAAGCTGCT GCAGCCCTGC 
CAGTTTGACT ATGCCATTTT CTGCCCTAAC CTGGCAGAGA TGGCATCAAC AGACAATGCA 
GACCAACAGA ACTTCACAGT GACACTGGAC CAGGTGCTGC TCCGCTGCCT CGCCCACCAA 
CAGCACTGGA GTCGCCTGCA TGAGGAGCAG GTCAGCCCGG ACCCCTGGAG CACCCCCGGC 
CAGGAGCAGG ATGGGCCCGC ATCTCTGCTG CTGGCGCCTC ACCCACCCCA CACCCACAGT 
GCCAGCTCCC TCGTCTTCAG CTGCATCTCC CATGCCTTGC AGTGGATCAC CCAAGGCCGG 
GACCCTCTCT TTCAGCCGCC CAGTCCCCCA CGGGGCCTCC TCGCCCACCC TGTGGCAGAC 
AGTGGGGCCA CGGTACTCCA AGAGGCTGCT GCCATTCACG TGCTGGTCAC AGGCAGCCTG 
CACCTGGTGG GCGGTGTCCT GAAGCTGCTG GAGCCCTCCC TGTCCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGS ( XM_024998399.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854167.1 Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa220189
Clone ID Related Accession (Same CDS sequence)	XM_024998399.1
Accession Version	XM_024998399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC CAGGCCTATC GGGGTGTCCT CTCTCGGCTA AAACTGCCTC CTTCCAGGTG 
CGTGAGCGGA TCCGCATCAA TGGGCAGCCC ATAAGCTCCG AGCTCTTCAC CAAGCACTTC 
TGGCGCCTCT ACCACCGGCT GGAGGAGACC AAGGACGACA GCAGCTGTGT CTCCATGCCC 
GGCTACTTCC GCTTCCTCAC GCTCATGGCC TTCCACGTCT TCCTCCAAGA GAAGGTAGAC 
CTGGCAGTGT TGGAAGTAGG CATTGGCGGG GCTTACGACT GCACCAACAT CATCAGGAAG 
CCTGTGGTGT GTGGGATCAC CTCTCTTGGC ATCGACCACA CTGGCCTTCT GGGGGACACG 
ATGGAGAAGA TCGCATGGCA GAAAGGGGGC ATTTTTAAGA GTGGCGTCCC TGCCTTCACC 
GTGCTCCAGC CTGACGAGCC GCTGGCGGTG CTAAGGGACA GGGCTCAGCA GATCTCATGT 
CCCCTCTACC TGTGCCCACC ACTACAAGCC CTGGAGGAAG GGGGGCCACC ACTGACCCTG 
GGCCTGGAGG GAGAGCACCA GCGGTCCAAT GCTGCCCTGG CCTTGCAGGT GGCCCGCTGC 
TGGCTGCAGC AGAAAGGTTA CCAGGATGCT GGGGAGCTGA AGGCATCCAG GCCCAGCCTC 
CCGGGGCAGC TGCCCCTGGC ACCTGTGTTC CAGCCCACGC CCCGCATGCA GCAGGGGCTT 
CGGCACACGG AATGGCCAGG TCGGACGCAG TTGCTGCGGC GCGGGCCTCT TACCTGGTAC 
CTGGACGGCG CGCACACCAC CAGCAGCATG CAAGCCTGTG TGCGCTGGTT CCGCCAGGCG 
CTGCACCGCT GCGAGAGGCC GGACAGCGGT TCCGAGGTGC GCGTCTTGCT CTTCAACTCC 
ACTGGGGACC GGGATTCCGC GGCCCTGCTG AAGCTGCTGC AGCCCTGCCA GTTTGACTAT 
GCCATTTTCT GCCCTAACCT GGCAGAGATG GCATCAACAG ACAATGCAGA CCAACAGAAC 
TTCACAGTGA CACTGGACCA GGTGCTGCTC CGCTGCCTCG CCCACCAACA GCACTGGAGT 
CGCCTGCATG AGGAGCAGGT CAGCCCGGAC CCCTGGAGCA CCCCCGGCCA GGAGCAGGAT 
GGGCCCGCAT CTCTGCTGCT GGCGCCTCAC CCACCCCACA CCCACAGTGC CAGCTCCCTC 
GTCTTCAGCT GCATCTCCCA TGCCTTGCAG TGGATCACCC AAGGCCGGGA CCCTCTCTTT 
CAGCCGCCCA GTCCCCCACG GGGCCTCCTC GCCCACCCTG TGGCAGACAG TGGGGCCACG 
GTACTCCAAG AGGCTGCTGC CATTCACGTG CTGGTCACAG GCAGCCTGCA CCTGGTGGGC 
GGTGTCCTGA AGCTGCTGGA GCCCTCCCTG TCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854167.1
CDS	17..1435
Translation	MEEPGLSGCPLSAKTASFQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAAAIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	XM_024998399.1
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998399.1

ORF Insert Sequence:

ATGGAGGAGC CAGGCCTATC GGGGTGTCCT CTCTCGGCTA AAACTGCCTC CTTCCAGGTG 
CGTGAGCGGA TCCGCATCAA TGGGCAGCCC ATAAGCTCCG AGCTCTTCAC CAAGCACTTC 
TGGCGCCTCT ACCACCGGCT GGAGGAGACC AAGGACGACA GCAGCTGTGT CTCCATGCCC 
GGCTACTTCC GCTTCCTCAC GCTCATGGCC TTCCACGTCT TCCTCCAAGA GAAGGTAGAC 
CTGGCAGTGT TGGAAGTAGG CATTGGCGGG GCTTACGACT GCACCAACAT CATCAGGAAG 
CCTGTGGTGT GTGGGATCAC CTCTCTTGGC ATCGACCACA CTGGCCTTCT GGGGGACACG 
ATGGAGAAGA TCGCATGGCA GAAAGGGGGC ATTTTTAAGA GTGGCGTCCC TGCCTTCACC 
GTGCTCCAGC CTGACGAGCC GCTGGCGGTG CTAAGGGACA GGGCTCAGCA GATCTCATGT 
CCCCTCTACC TGTGCCCACC ACTACAAGCC CTGGAGGAAG GGGGGCCACC ACTGACCCTG 
GGCCTGGAGG GAGAGCACCA GCGGTCCAAT GCTGCCCTGG CCTTGCAGGT GGCCCGCTGC 
TGGCTGCAGC AGAAAGGTTA CCAGGATGCT GGGGAGCTGA AGGCATCCAG GCCCAGCCTC 
CCGGGGCAGC TGCCCCTGGC ACCTGTGTTC CAGCCCACGC CCCGCATGCA GCAGGGGCTT 
CGGCACACGG AATGGCCAGG TCGGACGCAG TTGCTGCGGC GCGGGCCTCT TACCTGGTAC 
CTGGACGGCG CGCACACCAC CAGCAGCATG CAAGCCTGTG TGCGCTGGTT CCGCCAGGCG 
CTGCACCGCT GCGAGAGGCC GGACAGCGGT TCCGAGGTGC GCGTCTTGCT CTTCAACTCC 
ACTGGGGACC GGGATTCCGC GGCCCTGCTG AAGCTGCTGC AGCCCTGCCA GTTTGACTAT 
GCCATTTTCT GCCCTAACCT GGCAGAGATG GCATCAACAG ACAATGCAGA CCAACAGAAC 
TTCACAGTGA CACTGGACCA GGTGCTGCTC CGCTGCCTCG CCCACCAACA GCACTGGAGT 
CGCCTGCATG AGGAGCAGGT CAGCCCGGAC CCCTGGAGCA CCCCCGGCCA GGAGCAGGAT 
GGGCCCGCAT CTCTGCTGCT GGCGCCTCAC CCACCCCACA CCCACAGTGC CAGCTCCCTC 
GTCTTCAGCT GCATCTCCCA TGCCTTGCAG TGGATCACCC AAGGCCGGGA CCCTCTCTTT 
CAGCCGCCCA GTCCCCCACG GGGCCTCCTC GCCCACCCTG TGGCAGACAG TGGGGCCACG 
GTACTCCAAG AGGCTGCTGC CATTCACGTG CTGGTCACAG GCAGCCTGCA CCTGGTGGGC 
GGTGTCCTGA AGCTGCTGGA GCCCTCCCTG TCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGS ( XM_024998394.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854162.1 Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220185
Clone ID Related Accession (Same CDS sequence)	XM_024998394.1
Accession Version	XM_024998394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC CAGGCCTATC GGGGTGTCCT CTCTCGGCTA AAACTGCCTC CTTCCAGGAT 
GCCGTGCGTA CACTCAACAC CCTGCAGACC AATGCTAACT ACTTGGAGAT GGTGAAGCGT 
AAGCGGGGTG ACCCCCAGAC ACAGCTGGAA GCCATGAAGC TGTACTTGGC ACGGAGTGGG 
CTGCAGGTGG AGGACTTGGA CCAGCTGAAC ATTATCCACA TCACTGGAAC CAAGGGGAAG 
GGCTCCACGT GTGCCTTCAC TGAACGTATC CTCCGAAGCT ATGGCCTGAA GACGGGGTTC 
TTTAGCTCTC CCCACCTGGT GCAGGTGCGT GAGCGGATCC GCATCAATGG GCAGCCCATA 
AGCTCCGAGC TCTTCACCAA GCACTTCTGG CGCCTCTACC ACCGGCTGGA GGAGACCAAG 
GACGACAGCA GCTGTGTCTC CATGCCCGGC TACTTCCGCT TCCTCACGCT CATGGCCTTC 
CACGTCTTCC TCCAAGAGAA GGTAGACCTG GCAGTGTTGG AAGTAGGCAT TGGCGGGGCT 
TACGACTGCA CCAACATCAT CAGGAAGCCT GTGGTGTGTG GGATCACCTC TCTTGGCATC 
GACCACACTG GCCTTCTGGG GGACACGATG GAGAAGATCG CATGGCAGAA AGGGGGCATT 
TTTAAGAGTG GCGTCCCTGC CTTCACCGTG CTCCAGCCTG ACGAGCCGCT GGCGGTGCTA 
AGGGACAGGG CTCAGCAGAT CTCATGTCCC CTCTACCTGT GCCCACCACT ACAAGCCCTG 
GAGGAAGGGG GGCCACCACT GACCCTGGGC CTGGAGGGAG AGCACCAGCG GTCCAATGCT 
GCCCTGGCCT TGCAGGTGGC CCGCTGCTGG CTGCAGCAGA AAGGTTACCA GGATGCTGGG 
GAGCTGAAGG CATCCAGGCC CAGCCTCCCG GGGCAGCTGC CCCTGGCACC TGTGTTCCAG 
CCCACGCCCC GCATGCAGCA GGGGCTTCGG CACACGGAAT GGCCAGGTCG GACGCAGTTG 
CTGCGGCGCG GGCCTCTTAC CTGGTACCTG GACGGCGCGC ACACCACCAG CAGCATGCAA 
GCCTGTGTGC GCTGGTTCCG CCAGGCGCTG CACCGCTGCG AGAGGCCGGA CAGCGGTTCC 
GAGGTGCGCG TCTTGCTCTT CAACTCCACT GGGGACCGGG ATTCCGCGGC CCTGCTGAAG 
CTGCTGCAGC CCTGCCAGTT TGACTATGCC ATTTTCTGCC CTAACCTGGC AGAGATGGCA 
TCAACAGACA ATGCAGACCA ACAGAACTTC ACAGTGACAC TGGACCAGGT GCTGCTCCGC 
TGCCTCGCCC ACCAACAGCA CTGGAGTCGC CTGCATGAGG AGCAGGTCAG CCCGGACCCC 
TGGAGCACCC CCGGCCAGGA GCAGGATGGG CCCGCATCTC TGCTGCTGGC GCCTCACCCA 
CCCCACACCC ACAGTGCCAG CTCCCTCGTC TTCAGCTGCA TCTCCCATGC CTTGCAGTGG 
ATCACCCAAG GCCGGGACCC TCTCTTTCAG CCGCCCAGTC CCCCACGGGG CCTCCTCGCC 
CACCCTGTGG CAGACAGTGG GGCCACGGTA CTCCAAGAGG CTGCTGCCAT TCACGTGCTG 
GTCACAGGCA GCCTGCACCT GGTGGGCGGT GTCCTGAAGC TGCTGGAGCC CTCCCTGTCC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854162.1
CDS	40..1725
Translation	MEEPGLSGCPLSAKTASFQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAAAIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	XM_024998394.1
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998394.1

ORF Insert Sequence:

ATGGAGGAGC CAGGCCTATC GGGGTGTCCT CTCTCGGCTA AAACTGCCTC CTTCCAGGAT 
GCCGTGCGTA CACTCAACAC CCTGCAGACC AATGCTAACT ACTTGGAGAT GGTGAAGCGT 
AAGCGGGGTG ACCCCCAGAC ACAGCTGGAA GCCATGAAGC TGTACTTGGC ACGGAGTGGG 
CTGCAGGTGG AGGACTTGGA CCAGCTGAAC ATTATCCACA TCACTGGAAC CAAGGGGAAG 
GGCTCCACGT GTGCCTTCAC TGAACGTATC CTCCGAAGCT ATGGCCTGAA GACGGGGTTC 
TTTAGCTCTC CCCACCTGGT GCAGGTGCGT GAGCGGATCC GCATCAATGG GCAGCCCATA 
AGCTCCGAGC TCTTCACCAA GCACTTCTGG CGCCTCTACC ACCGGCTGGA GGAGACCAAG 
GACGACAGCA GCTGTGTCTC CATGCCCGGC TACTTCCGCT TCCTCACGCT CATGGCCTTC 
CACGTCTTCC TCCAAGAGAA GGTAGACCTG GCAGTGTTGG AAGTAGGCAT TGGCGGGGCT 
TACGACTGCA CCAACATCAT CAGGAAGCCT GTGGTGTGTG GGATCACCTC TCTTGGCATC 
GACCACACTG GCCTTCTGGG GGACACGATG GAGAAGATCG CATGGCAGAA AGGGGGCATT 
TTTAAGAGTG GCGTCCCTGC CTTCACCGTG CTCCAGCCTG ACGAGCCGCT GGCGGTGCTA 
AGGGACAGGG CTCAGCAGAT CTCATGTCCC CTCTACCTGT GCCCACCACT ACAAGCCCTG 
GAGGAAGGGG GGCCACCACT GACCCTGGGC CTGGAGGGAG AGCACCAGCG GTCCAATGCT 
GCCCTGGCCT TGCAGGTGGC CCGCTGCTGG CTGCAGCAGA AAGGTTACCA GGATGCTGGG 
GAGCTGAAGG CATCCAGGCC CAGCCTCCCG GGGCAGCTGC CCCTGGCACC TGTGTTCCAG 
CCCACGCCCC GCATGCAGCA GGGGCTTCGG CACACGGAAT GGCCAGGTCG GACGCAGTTG 
CTGCGGCGCG GGCCTCTTAC CTGGTACCTG GACGGCGCGC ACACCACCAG CAGCATGCAA 
GCCTGTGTGC GCTGGTTCCG CCAGGCGCTG CACCGCTGCG AGAGGCCGGA CAGCGGTTCC 
GAGGTGCGCG TCTTGCTCTT CAACTCCACT GGGGACCGGG ATTCCGCGGC CCTGCTGAAG 
CTGCTGCAGC CCTGCCAGTT TGACTATGCC ATTTTCTGCC CTAACCTGGC AGAGATGGCA 
TCAACAGACA ATGCAGACCA ACAGAACTTC ACAGTGACAC TGGACCAGGT GCTGCTCCGC 
TGCCTCGCCC ACCAACAGCA CTGGAGTCGC CTGCATGAGG AGCAGGTCAG CCCGGACCCC 
TGGAGCACCC CCGGCCAGGA GCAGGATGGG CCCGCATCTC TGCTGCTGGC GCCTCACCCA 
CCCCACACCC ACAGTGCCAG CTCCCTCGTC TTCAGCTGCA TCTCCCATGC CTTGCAGTGG 
ATCACCCAAG GCCGGGACCC TCTCTTTCAG CCGCCCAGTC CCCCACGGGG CCTCCTCGCC 
CACCCTGTGG CAGACAGTGG GGCCACGGTA CTCCAAGAGG CTGCTGCCAT TCACGTGCTG 
GTCACAGGCA GCCTGCACCT GGTGGGCGGT GTCCTGAAGC TGCTGGAGCC CTCCCTGTCC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGS ( XM_024998395.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854163.1 Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220186
Clone ID Related Accession (Same CDS sequence)	XM_024998395.1 , XM_024998396.1
Accession Version	XM_024998395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTTA ACTTGCTACC TGGGGCTCAC TGCCAGGATG CCGTGCGTAC ACTCAACACC 
CTGCAGACCA ATGCTAACTA CTTGGAGATG GTGAAGCGTA AGCGGGGTGA CCCCCAGACA 
CAGCTGGAAG CCATGAAGCT GTACTTGGCA CGGAGTGGGC TGCAGGTGGA GGACTTGGAC 
CAGCTGAACA TTATCCACAT CACTGGAACC AAGGGGAAGG GCTCCACGTG TGCCTTCACT 
GAACGTATCC TCCGAAGCTA TGGCCTGAAG ACGGGGTTCT TTAGCTCTCC CCACCTGGTG 
CAGGTGCGTG AGCGGATCCG CATCAATGGG CAGCCCATAA GCTCCGAGCT CTTCACCAAG 
CACTTCTGGC GCCTCTACCA CCGGCTGGAG GAGACCAAGG ACGACAGCAG CTGTGTCTCC 
ATGCCCGGCT ACTTCCGCTT CCTCACGCTC ATGGCCTTCC ACGTCTTCCT CCAAGAGAAG 
GTAGACCTGG CAGTGTTGGA AGTAGGCATT GGCGGGGCTT ACGACTGCAC CAACATCATC 
AGGAAGCCTG TGGTGTGTGG GATCACCTCT CTTGGCATCG ACCACACTGG CCTTCTGGGG 
GACACGATGG AGAAGATCGC ATGGCAGAAA GGGGGCATTT TTAAGAGTGG CGTCCCTGCC 
TTCACCGTGC TCCAGCCTGA CGAGCCGCTG GCGGTGCTAA GGGACAGGGC TCAGCAGATC 
TCATGTCCCC TCTACCTGTG CCCACCACTA CAAGCCCTGG AGGAAGGGGG GCCACCACTG 
ACCCTGGGCC TGGAGGGAGA GCACCAGCGG TCCAATGCTG CCCTGGCCTT GCAGGTGGCC 
CGCTGCTGGC TGCAGCAGAA AGGTTACCAG GATGCTGGGG AGCTGAAGGC ATCCAGGCCC 
AGCCTCCCGG GGCAGCTGCC CCTGGCACCT GTGTTCCAGC CCACGCCCCG CATGCAGCAG 
GGGCTTCGGC ACACGGAATG GCCAGGTCGG ACGCAGTTGC TGCGGCGCGG GCCTCTTACC 
TGGTACCTGG ACGGCGCGCA CACCACCAGC AGCATGCAAG CCTGTGTGCG CTGGTTCCGC 
CAGGCGCTGC ACCGCTGCGA GAGGCCGGAC AGCGGTTCCG AGGTGCGCGT CTTGCTCTTC 
AACTCCACTG GGGACCGGGA TTCCGCGGCC CTGCTGAAGC TGCTGCAGCC CTGCCAGTTT 
GACTATGCCA TTTTCTGCCC TAACCTGGCA GAGATGGCAT CAACAGACAA TGCAGACCAA 
CAGAACTTCA CAGTGACACT GGACCAGGTG CTGCTCCGCT GCCTCGCCCA CCAACAGCAC 
TGGAGTCGCC TGCATGAGGA GCAGGTCAGC CCGGACCCCT GGAGCACCCC CGGCCAGGAG 
CAGGATGGGC CCGCATCTCT GCTGCTGGCG CCTCACCCAC CCCACACCCA CAGTGCCAGC 
TCCCTCGTCT TCAGCTGCAT CTCCCATGCC TTGCAGTGGA TCACCCAAGG CCGGGACCCT 
CTCTTTCAGC CGCCCAGTCC CCCACGGGGC CTCCTCGCCC ACCCTGTGGC AGACAGTGGG 
GCCACGGTAC TCCAAGAGGC TGCTGCCATT CACGTGCTGG TCACAGGCAG CCTGCACCTG 
GTGGGCGGTG TCCTGAAGCT GCTGGAGCCC TCCCTGTCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854163.1
CDS	307..1971
Translation	MRLNLLPGAHCQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAAAIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	XM_024998395.1
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998395.1

ORF Insert Sequence:

ATGCGCCTTA ACTTGCTACC TGGGGCTCAC TGCCAGGATG CCGTGCGTAC ACTCAACACC 
CTGCAGACCA ATGCTAACTA CTTGGAGATG GTGAAGCGTA AGCGGGGTGA CCCCCAGACA 
CAGCTGGAAG CCATGAAGCT GTACTTGGCA CGGAGTGGGC TGCAGGTGGA GGACTTGGAC 
CAGCTGAACA TTATCCACAT CACTGGAACC AAGGGGAAGG GCTCCACGTG TGCCTTCACT 
GAACGTATCC TCCGAAGCTA TGGCCTGAAG ACGGGGTTCT TTAGCTCTCC CCACCTGGTG 
CAGGTGCGTG AGCGGATCCG CATCAATGGG CAGCCCATAA GCTCCGAGCT CTTCACCAAG 
CACTTCTGGC GCCTCTACCA CCGGCTGGAG GAGACCAAGG ACGACAGCAG CTGTGTCTCC 
ATGCCCGGCT ACTTCCGCTT CCTCACGCTC ATGGCCTTCC ACGTCTTCCT CCAAGAGAAG 
GTAGACCTGG CAGTGTTGGA AGTAGGCATT GGCGGGGCTT ACGACTGCAC CAACATCATC 
AGGAAGCCTG TGGTGTGTGG GATCACCTCT CTTGGCATCG ACCACACTGG CCTTCTGGGG 
GACACGATGG AGAAGATCGC ATGGCAGAAA GGGGGCATTT TTAAGAGTGG CGTCCCTGCC 
TTCACCGTGC TCCAGCCTGA CGAGCCGCTG GCGGTGCTAA GGGACAGGGC TCAGCAGATC 
TCATGTCCCC TCTACCTGTG CCCACCACTA CAAGCCCTGG AGGAAGGGGG GCCACCACTG 
ACCCTGGGCC TGGAGGGAGA GCACCAGCGG TCCAATGCTG CCCTGGCCTT GCAGGTGGCC 
CGCTGCTGGC TGCAGCAGAA AGGTTACCAG GATGCTGGGG AGCTGAAGGC ATCCAGGCCC 
AGCCTCCCGG GGCAGCTGCC CCTGGCACCT GTGTTCCAGC CCACGCCCCG CATGCAGCAG 
GGGCTTCGGC ACACGGAATG GCCAGGTCGG ACGCAGTTGC TGCGGCGCGG GCCTCTTACC 
TGGTACCTGG ACGGCGCGCA CACCACCAGC AGCATGCAAG CCTGTGTGCG CTGGTTCCGC 
CAGGCGCTGC ACCGCTGCGA GAGGCCGGAC AGCGGTTCCG AGGTGCGCGT CTTGCTCTTC 
AACTCCACTG GGGACCGGGA TTCCGCGGCC CTGCTGAAGC TGCTGCAGCC CTGCCAGTTT 
GACTATGCCA TTTTCTGCCC TAACCTGGCA GAGATGGCAT CAACAGACAA TGCAGACCAA 
CAGAACTTCA CAGTGACACT GGACCAGGTG CTGCTCCGCT GCCTCGCCCA CCAACAGCAC 
TGGAGTCGCC TGCATGAGGA GCAGGTCAGC CCGGACCCCT GGAGCACCCC CGGCCAGGAG 
CAGGATGGGC CCGCATCTCT GCTGCTGGCG CCTCACCCAC CCCACACCCA CAGTGCCAGC 
TCCCTCGTCT TCAGCTGCAT CTCCCATGCC TTGCAGTGGA TCACCCAAGG CCGGGACCCT 
CTCTTTCAGC CGCCCAGTCC CCCACGGGGC CTCCTCGCCC ACCCTGTGGC AGACAGTGGG 
GCCACGGTAC TCCAAGAGGC TGCTGCCATT CACGTGCTGG TCACAGGCAG CCTGCACCTG 
GTGGGCGGTG TCCTGAAGCT GCTGGAGCCC TCCCTGTCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGS ( XM_024998396.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854164.1 Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220186
Clone ID Related Accession (Same CDS sequence)	XM_024998395.1 , XM_024998396.1
Accession Version	XM_024998396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTTA ACTTGCTACC TGGGGCTCAC TGCCAGGATG CCGTGCGTAC ACTCAACACC 
CTGCAGACCA ATGCTAACTA CTTGGAGATG GTGAAGCGTA AGCGGGGTGA CCCCCAGACA 
CAGCTGGAAG CCATGAAGCT GTACTTGGCA CGGAGTGGGC TGCAGGTGGA GGACTTGGAC 
CAGCTGAACA TTATCCACAT CACTGGAACC AAGGGGAAGG GCTCCACGTG TGCCTTCACT 
GAACGTATCC TCCGAAGCTA TGGCCTGAAG ACGGGGTTCT TTAGCTCTCC CCACCTGGTG 
CAGGTGCGTG AGCGGATCCG CATCAATGGG CAGCCCATAA GCTCCGAGCT CTTCACCAAG 
CACTTCTGGC GCCTCTACCA CCGGCTGGAG GAGACCAAGG ACGACAGCAG CTGTGTCTCC 
ATGCCCGGCT ACTTCCGCTT CCTCACGCTC ATGGCCTTCC ACGTCTTCCT CCAAGAGAAG 
GTAGACCTGG CAGTGTTGGA AGTAGGCATT GGCGGGGCTT ACGACTGCAC CAACATCATC 
AGGAAGCCTG TGGTGTGTGG GATCACCTCT CTTGGCATCG ACCACACTGG CCTTCTGGGG 
GACACGATGG AGAAGATCGC ATGGCAGAAA GGGGGCATTT TTAAGAGTGG CGTCCCTGCC 
TTCACCGTGC TCCAGCCTGA CGAGCCGCTG GCGGTGCTAA GGGACAGGGC TCAGCAGATC 
TCATGTCCCC TCTACCTGTG CCCACCACTA CAAGCCCTGG AGGAAGGGGG GCCACCACTG 
ACCCTGGGCC TGGAGGGAGA GCACCAGCGG TCCAATGCTG CCCTGGCCTT GCAGGTGGCC 
CGCTGCTGGC TGCAGCAGAA AGGTTACCAG GATGCTGGGG AGCTGAAGGC ATCCAGGCCC 
AGCCTCCCGG GGCAGCTGCC CCTGGCACCT GTGTTCCAGC CCACGCCCCG CATGCAGCAG 
GGGCTTCGGC ACACGGAATG GCCAGGTCGG ACGCAGTTGC TGCGGCGCGG GCCTCTTACC 
TGGTACCTGG ACGGCGCGCA CACCACCAGC AGCATGCAAG CCTGTGTGCG CTGGTTCCGC 
CAGGCGCTGC ACCGCTGCGA GAGGCCGGAC AGCGGTTCCG AGGTGCGCGT CTTGCTCTTC 
AACTCCACTG GGGACCGGGA TTCCGCGGCC CTGCTGAAGC TGCTGCAGCC CTGCCAGTTT 
GACTATGCCA TTTTCTGCCC TAACCTGGCA GAGATGGCAT CAACAGACAA TGCAGACCAA 
CAGAACTTCA CAGTGACACT GGACCAGGTG CTGCTCCGCT GCCTCGCCCA CCAACAGCAC 
TGGAGTCGCC TGCATGAGGA GCAGGTCAGC CCGGACCCCT GGAGCACCCC CGGCCAGGAG 
CAGGATGGGC CCGCATCTCT GCTGCTGGCG CCTCACCCAC CCCACACCCA CAGTGCCAGC 
TCCCTCGTCT TCAGCTGCAT CTCCCATGCC TTGCAGTGGA TCACCCAAGG CCGGGACCCT 
CTCTTTCAGC CGCCCAGTCC CCCACGGGGC CTCCTCGCCC ACCCTGTGGC AGACAGTGGG 
GCCACGGTAC TCCAAGAGGC TGCTGCCATT CACGTGCTGG TCACAGGCAG CCTGCACCTG 
GTGGGCGGTG TCCTGAAGCT GCTGGAGCCC TCCCTGTCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854164.1
CDS	278..1942
Translation	MRLNLLPGAHCQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAAAIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	XM_024998396.1
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998396.1

ORF Insert Sequence:

ATGCGCCTTA ACTTGCTACC TGGGGCTCAC TGCCAGGATG CCGTGCGTAC ACTCAACACC 
CTGCAGACCA ATGCTAACTA CTTGGAGATG GTGAAGCGTA AGCGGGGTGA CCCCCAGACA 
CAGCTGGAAG CCATGAAGCT GTACTTGGCA CGGAGTGGGC TGCAGGTGGA GGACTTGGAC 
CAGCTGAACA TTATCCACAT CACTGGAACC AAGGGGAAGG GCTCCACGTG TGCCTTCACT 
GAACGTATCC TCCGAAGCTA TGGCCTGAAG ACGGGGTTCT TTAGCTCTCC CCACCTGGTG 
CAGGTGCGTG AGCGGATCCG CATCAATGGG CAGCCCATAA GCTCCGAGCT CTTCACCAAG 
CACTTCTGGC GCCTCTACCA CCGGCTGGAG GAGACCAAGG ACGACAGCAG CTGTGTCTCC 
ATGCCCGGCT ACTTCCGCTT CCTCACGCTC ATGGCCTTCC ACGTCTTCCT CCAAGAGAAG 
GTAGACCTGG CAGTGTTGGA AGTAGGCATT GGCGGGGCTT ACGACTGCAC CAACATCATC 
AGGAAGCCTG TGGTGTGTGG GATCACCTCT CTTGGCATCG ACCACACTGG CCTTCTGGGG 
GACACGATGG AGAAGATCGC ATGGCAGAAA GGGGGCATTT TTAAGAGTGG CGTCCCTGCC 
TTCACCGTGC TCCAGCCTGA CGAGCCGCTG GCGGTGCTAA GGGACAGGGC TCAGCAGATC 
TCATGTCCCC TCTACCTGTG CCCACCACTA CAAGCCCTGG AGGAAGGGGG GCCACCACTG 
ACCCTGGGCC TGGAGGGAGA GCACCAGCGG TCCAATGCTG CCCTGGCCTT GCAGGTGGCC 
CGCTGCTGGC TGCAGCAGAA AGGTTACCAG GATGCTGGGG AGCTGAAGGC ATCCAGGCCC 
AGCCTCCCGG GGCAGCTGCC CCTGGCACCT GTGTTCCAGC CCACGCCCCG CATGCAGCAG 
GGGCTTCGGC ACACGGAATG GCCAGGTCGG ACGCAGTTGC TGCGGCGCGG GCCTCTTACC 
TGGTACCTGG ACGGCGCGCA CACCACCAGC AGCATGCAAG CCTGTGTGCG CTGGTTCCGC 
CAGGCGCTGC ACCGCTGCGA GAGGCCGGAC AGCGGTTCCG AGGTGCGCGT CTTGCTCTTC 
AACTCCACTG GGGACCGGGA TTCCGCGGCC CTGCTGAAGC TGCTGCAGCC CTGCCAGTTT 
GACTATGCCA TTTTCTGCCC TAACCTGGCA GAGATGGCAT CAACAGACAA TGCAGACCAA 
CAGAACTTCA CAGTGACACT GGACCAGGTG CTGCTCCGCT GCCTCGCCCA CCAACAGCAC 
TGGAGTCGCC TGCATGAGGA GCAGGTCAGC CCGGACCCCT GGAGCACCCC CGGCCAGGAG 
CAGGATGGGC CCGCATCTCT GCTGCTGGCG CCTCACCCAC CCCACACCCA CAGTGCCAGC 
TCCCTCGTCT TCAGCTGCAT CTCCCATGCC TTGCAGTGGA TCACCCAAGG CCGGGACCCT 
CTCTTTCAGC CGCCCAGTCC CCCACGGGGC CTCCTCGCCC ACCCTGTGGC AGACAGTGGG 
GCCACGGTAC TCCAAGAGGC TGCTGCCATT CACGTGCTGG TCACAGGCAG CCTGCACCTG 
GTGGGCGGTG TCCTGAAGCT GCTGGAGCCC TCCCTGTCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGS ( XM_024998397.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854165.1 Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X4, mRNA.

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CloneID	OBa220187
Clone ID Related Accession (Same CDS sequence)	XM_024998397.1
Accession Version	XM_024998397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	folylpolyglutamate synthase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCAG AAGCCTATCA GGATGCCGTG CGTACACTCA ACACCCTGCA GACCAATGCT 
AACTACTTGG AGATGGTGAA GCGTAAGCGG GGTGACCCCC AGACACAGCT GGAAGCCATG 
AAGCTGTACT TGGCACGGAG TGGGCTGCAG GTGGAGGACT TGGACCAGCT GAACATTATC 
CACATCACTG GAACCAAGGG GAAGGGCTCC ACGTGTGCCT TCACTGAACG TATCCTCCGA 
AGCTATGGCC TGAAGACGGG GTTCTTTAGC TCTCCCCACC TGGTGCAGGT GCGTGAGCGG 
ATCCGCATCA ATGGGCAGCC CATAAGCTCC GAGCTCTTCA CCAAGCACTT CTGGCGCCTC 
TACCACCGGC TGGAGGAGAC CAAGGACGAC AGCAGCTGTG TCTCCATGCC CGGCTACTTC 
CGCTTCCTCA CGCTCATGGC CTTCCACGTC TTCCTCCAAG AGAAGGTAGA CCTGGCAGTG 
TTGGAAGTAG GCATTGGCGG GGCTTACGAC TGCACCAACA TCATCAGGAA GCCTGTGGTG 
TGTGGGATCA CCTCTCTTGG CATCGACCAC ACTGGCCTTC TGGGGGACAC GATGGAGAAG 
ATCGCATGGC AGAAAGGGGG CATTTTTAAG AGTGGCGTCC CTGCCTTCAC CGTGCTCCAG 
CCTGACGAGC CGCTGGCGGT GCTAAGGGAC AGGGCTCAGC AGATCTCATG TCCCCTCTAC 
CTGTGCCCAC CACTACAAGC CCTGGAGGAA GGGGGGCCAC CACTGACCCT GGGCCTGGAG 
GGAGAGCACC AGCGGTCCAA TGCTGCCCTG GCCTTGCAGG TGGCCCGCTG CTGGCTGCAG 
CAGAAAGGTT ACCAGGATGC TGGGGAGCTG AAGGCATCCA GGCCCAGCCT CCCGGGGCAG 
CTGCCCCTGG CACCTGTGTT CCAGCCCACG CCCCGCATGC AGCAGGGGCT TCGGCACACG 
GAATGGCCAG GTCGGACGCA GTTGCTGCGG CGCGGGCCTC TTACCTGGTA CCTGGACGGC 
GCGCACACCA CCAGCAGCAT GCAAGCCTGT GTGCGCTGGT TCCGCCAGGC GCTGCACCGC 
TGCGAGAGGC CGGACAGCGG TTCCGAGGTG CGCGTCTTGC TCTTCAACTC CACTGGGGAC 
CGGGATTCCG CGGCCCTGCT GAAGCTGCTG CAGCCCTGCC AGTTTGACTA TGCCATTTTC 
TGCCCTAACC TGGCAGAGAT GGCATCAACA GACAATGCAG ACCAACAGAA CTTCACAGTG 
ACACTGGACC AGGTGCTGCT CCGCTGCCTC GCCCACCAAC AGCACTGGAG TCGCCTGCAT 
GAGGAGCAGG TCAGCCCGGA CCCCTGGAGC ACCCCCGGCC AGGAGCAGGA TGGGCCCGCA 
TCTCTGCTGC TGGCGCCTCA CCCACCCCAC ACCCACAGTG CCAGCTCCCT CGTCTTCAGC 
TGCATCTCCC ATGCCTTGCA GTGGATCACC CAAGGCCGGG ACCCTCTCTT TCAGCCGCCC 
AGTCCCCCAC GGGGCCTCCT CGCCCACCCT GTGGCAGACA GTGGGGCCAC GGTACTCCAA 
GAGGCTGCTG CCATTCACGT GCTGGTCACA GGCAGCCTGC ACCTGGTGGG CGGTGTCCTG 
AAGCTGCTGG AGCCCTCCCT GTCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854165.1
CDS	57..1706
Translation	MKAEAYQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLLRRGPLTWYLDGAHTTSSMQACVRWFRQALHRCERPDSGSEVRVLLFNSTGDRDSAALLKLLQPCQFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAAAIHVLVTGSLHLVGGVLKLLEPSLSQ

Target ORF information:

RefSeq Version	XM_024998397.1
Organism	Bos taurus(cattle)
Definition	Bos taurus folylpolyglutamate synthase (FPGS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998397.1

ORF Insert Sequence:

ATGAAAGCAG AAGCCTATCA GGATGCCGTG CGTACACTCA ACACCCTGCA GACCAATGCT 
AACTACTTGG AGATGGTGAA GCGTAAGCGG GGTGACCCCC AGACACAGCT GGAAGCCATG 
AAGCTGTACT TGGCACGGAG TGGGCTGCAG GTGGAGGACT TGGACCAGCT GAACATTATC 
CACATCACTG GAACCAAGGG GAAGGGCTCC ACGTGTGCCT TCACTGAACG TATCCTCCGA 
AGCTATGGCC TGAAGACGGG GTTCTTTAGC TCTCCCCACC TGGTGCAGGT GCGTGAGCGG 
ATCCGCATCA ATGGGCAGCC CATAAGCTCC GAGCTCTTCA CCAAGCACTT CTGGCGCCTC 
TACCACCGGC TGGAGGAGAC CAAGGACGAC AGCAGCTGTG TCTCCATGCC CGGCTACTTC 
CGCTTCCTCA CGCTCATGGC CTTCCACGTC TTCCTCCAAG AGAAGGTAGA CCTGGCAGTG 
TTGGAAGTAG GCATTGGCGG GGCTTACGAC TGCACCAACA TCATCAGGAA GCCTGTGGTG 
TGTGGGATCA CCTCTCTTGG CATCGACCAC ACTGGCCTTC TGGGGGACAC GATGGAGAAG 
ATCGCATGGC AGAAAGGGGG CATTTTTAAG AGTGGCGTCC CTGCCTTCAC CGTGCTCCAG 
CCTGACGAGC CGCTGGCGGT GCTAAGGGAC AGGGCTCAGC AGATCTCATG TCCCCTCTAC 
CTGTGCCCAC CACTACAAGC CCTGGAGGAA GGGGGGCCAC CACTGACCCT GGGCCTGGAG 
GGAGAGCACC AGCGGTCCAA TGCTGCCCTG GCCTTGCAGG TGGCCCGCTG CTGGCTGCAG 
CAGAAAGGTT ACCAGGATGC TGGGGAGCTG AAGGCATCCA GGCCCAGCCT CCCGGGGCAG 
CTGCCCCTGG CACCTGTGTT CCAGCCCACG CCCCGCATGC AGCAGGGGCT TCGGCACACG 
GAATGGCCAG GTCGGACGCA GTTGCTGCGG CGCGGGCCTC TTACCTGGTA CCTGGACGGC 
GCGCACACCA CCAGCAGCAT GCAAGCCTGT GTGCGCTGGT TCCGCCAGGC GCTGCACCGC 
TGCGAGAGGC CGGACAGCGG TTCCGAGGTG CGCGTCTTGC TCTTCAACTC CACTGGGGAC 
CGGGATTCCG CGGCCCTGCT GAAGCTGCTG CAGCCCTGCC AGTTTGACTA TGCCATTTTC 
TGCCCTAACC TGGCAGAGAT GGCATCAACA GACAATGCAG ACCAACAGAA CTTCACAGTG 
ACACTGGACC AGGTGCTGCT CCGCTGCCTC GCCCACCAAC AGCACTGGAG TCGCCTGCAT 
GAGGAGCAGG TCAGCCCGGA CCCCTGGAGC ACCCCCGGCC AGGAGCAGGA TGGGCCCGCA 
TCTCTGCTGC TGGCGCCTCA CCCACCCCAC ACCCACAGTG CCAGCTCCCT CGTCTTCAGC 
TGCATCTCCC ATGCCTTGCA GTGGATCACC CAAGGCCGGG ACCCTCTCTT TCAGCCGCCC 
AGTCCCCCAC GGGGCCTCCT CGCCCACCCT GTGGCAGACA GTGGGGCCAC GGTACTCCAA 
GAGGCTGCTG CCATTCACGT GCTGGTCACA GGCAGCCTGC ACCTGGTGGG CGGTGTCCTG 
AAGCTGCTGG AGCCCTCCCT GTCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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FPGS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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