PRRC2B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005213441.3	XP_005213498.1	protein PRRC2B isoform X1
XM_015465510.1	XP_015320996.1	protein PRRC2B isoform X1
XM_015465512.1	XP_015320998.1	protein PRRC2B isoform X3
XM_015465513.1	XP_015320999.1	protein PRRC2B isoform X4
XM_015465515.1	XP_015321001.1	protein PRRC2B isoform X6
XM_024999597.1	XP_024855365.1	protein PRRC2B isoform X4
XM_015465519.1	XP_015321005.1	protein PRRC2B isoform X10
XM_024999593.1	XP_024855361.1	protein PRRC2B isoform X1
XM_024999595.1	XP_024855363.1	protein PRRC2B isoform X2
XM_024999600.1	XP_024855368.1	protein PRRC2B isoform X7
XM_024999601.1	XP_024855369.1	protein PRRC2B isoform X8
XM_024999602.1	XP_024855370.1	protein PRRC2B isoform X9
XM_024999604.1	XP_024855372.1	protein PRRC2B isoform X11
XM_005213447.3	XP_005213504.1	protein PRRC2B isoform X10
XM_005213445.3	XP_005213502.1	protein PRRC2B isoform X5
XM_015465511.1	XP_015320997.1	protein PRRC2B isoform X2
XM_015465517.1	XP_015321003.1	protein PRRC2B isoform X8
XM_024999599.1	XP_024855367.1	protein PRRC2B isoform X6
XM_024999603.1	XP_024855371.1	protein PRRC2B isoform X10
XM_005213443.3	XP_005213500.1	protein PRRC2B isoform X3
XM_002691647.5	XP_002691693.1	protein PRRC2B isoform X6
XM_005213446.3	XP_005213503.1	protein PRRC2B isoform X7
XM_015465516.1	XP_015321002.1	protein PRRC2B isoform X7
XM_015465518.1	XP_015321004.1	protein PRRC2B isoform X9
XM_024999594.1	XP_024855362.1	protein PRRC2B isoform X1
XM_024999606.1	XP_024855374.1	protein PRRC2B isoform X13
XM_015465514.1	XP_015321000.1	protein PRRC2B isoform X5
XM_005213444.3	XP_005213501.1	protein PRRC2B isoform X4
XM_005213442.3	XP_005213499.1	protein PRRC2B isoform X2
XM_010810520.2	XP_010808822.1	protein PRRC2B isoform X8
XM_010810521.2	XP_010808823.1	protein PRRC2B isoform X9
XM_024999596.1	XP_024855364.1	protein PRRC2B isoform X3
XM_024999598.1	XP_024855366.1	protein PRRC2B isoform X5
XM_010810520.3	XP_010808822.1	protein PRRC2B isoform X10
XM_024999605.1	XP_024855373.1	protein PRRC2B isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa24522	XM_024999605.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24522	XM_015465519.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24522	XM_005213447.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46163	XM_015465517.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46163	XM_010810520.3 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46163	XM_024999603.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46164	XM_024999604.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46164	XM_015465518.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46164	XM_010810521.2 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa220952	XM_024999600.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa220953	XM_024999602.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa220954	XM_024999606.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa24515	XM_015465510.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24515	XM_005213441.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24515	XM_024999594.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24515	XM_024999593.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24516	XM_005213442.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24516	XM_015465511.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24516	XM_024999595.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24517	XM_024999596.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24517	XM_015465512.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24517	XM_005213443.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24518	XM_024999597.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24518	XM_015465513.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24518	XM_005213444.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24519	XM_015465514.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24519	XM_005213445.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24519	XM_024999598.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24520	XM_002691647.5 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24520	XM_015465515.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24520	XM_024999599.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24521	XM_005213446.3 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24521	XM_015465516.1 Latest version!	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa24521	XM_024999601.1 Latest version!	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa46163	XM_010810520.2	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

PRRC2B ( XM_015465519.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321005.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa24522
Clone ID Related Accession (Same CDS sequence)	XM_015465519.1 , XM_005213447.3 , XM_024999605.1
Accession Version	XM_015465519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321005.1
CDS	1412..7258
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVSF

Target ORF information:

RefSeq Version	XM_015465519.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465519.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213447.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213504.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.

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CloneID	OBa24522
Clone ID Related Accession (Same CDS sequence)	XM_015465519.1 , XM_005213447.3 , XM_024999605.1
Accession Version	XM_005213447.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213504.1
CDS	1412..7258
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVSF

Target ORF information:

RefSeq Version	XM_005213447.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213447.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999605.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855373.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X14, mRNA.

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CloneID	OBa24522
Clone ID Related Accession (Same CDS sequence)	XM_015465519.1 , XM_005213447.3 , XM_024999605.1
Accession Version	XM_024999605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855373.1
CDS	311..6157
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVSF

Target ORF information:

RefSeq Version	XM_024999605.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999605.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465517.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321003.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.

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CloneID	OBa46163
Clone ID Related Accession (Same CDS sequence)	XM_015465517.1 , XM_024999603.1 , XM_010810520.2 , XM_010810520.3
Accession Version	XM_015465517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321003.1
CDS	272..6145
Translation	MGQDQASALRMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465517.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465517.1

ORF Insert Sequence:

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999603.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855371.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X11, mRNA.

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CloneID	OBa46163
Clone ID Related Accession (Same CDS sequence)	XM_015465517.1 , XM_024999603.1 , XM_010810520.2 , XM_010810520.3
Accession Version	XM_024999603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855371.1
CDS	188..6061
Translation	MGQDQASALRMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999603.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999603.1

ORF Insert Sequence:

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_010810520.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808822.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.

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CloneID	OBa46163
Clone ID Related Accession (Same CDS sequence)	XM_015465517.1 , XM_024999603.1 , XM_010810520.2 , XM_010810520.3
Accession Version	XM_010810520.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808822.1
CDS	272..6145
Translation	MGQDQASALRMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_010810520.2
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810520.2

ORF Insert Sequence:

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_010810520.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808822.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X12, mRNA.

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CloneID	OBa46163
Clone ID Related Accession (Same CDS sequence)	XM_015465517.1 , XM_024999603.1 , XM_010810520.2 , XM_010810520.3
Accession Version	XM_010810520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810520.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808822.1
CDS	278..6151
Translation	MGQDQASALRMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_010810520.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810520.3

ORF Insert Sequence:

ATGGGCCAGG ACCAAGCCTC CGCCCTCAGA ATGTGTTCGC CACAGTCATC AGAGAACCAG 
GGTACAGTGG AACGAGGATC TTTTCCCCTT CCTCAGCTCC GTCTTGAACC CCGTGTTCCT 
TTTAGACAGT TCCAGATGAA TGACCAAGAC GGAAAAGAAA ATAGACTGGG AGTGAGCCGC 
CCGATCCGTC CACTGAGGCC GCTGGTGGAG CGGGTGCCAC GACCCACCAT CGTCAATGCA 
GAAAACCTGA AGGGCCTCGA TGACCTGGAC ACTGACGCCG ACGATGGCTG GGCAGGCCTC 
CATGAAGAAG TGGACTACTC CGAGAAGCTG AAGTTCAGTG ATGATGAGGA GGAGGAGGAA 
GTGTTGAAGG ACGGCAGGCC AAAGTGGAAC AGTTGGGACC CCAGGAGGCA GCGGCAGTTA 
TCCTTGAGCT CTGCAGACAG TGCTGATGCC AAGCGCACCC AAGAGGAAGG AAAAGACTGG 
AGTGAAACAG GAGGCGTGAC CCGTGTCGTC CGGAAGGTGC CGGAACCTCA GCCGCCTTCC 
AGGAAGCTGC ACAGCTGGGC GTCAGGCCCT GACTACCAGC AGAAATCCTC CATGGGCACT 
GTATTTCGGC AGCAGTCTGC CGAGGACAAA GAGGACAAGC CCCCACCGCG GCAGAAGTTT 
GTCCAGTCTG AGATGTCCGA GGCTGTGGAG CGAGCCCGGA AGCGCCGGGA GGAGGAGGAG 
CGCCGTGCGC GCGAGGAAAG GCTGGCTGCC TGTGCTGCCA AACTCAAGCA GCTTGACCAG 
AAGTGCAAGC AGGCTCAGCG GGCCAGCGAG GCCCAGAAGC AGGCGGAGAA GGAAGCTCCC 
CGCTCTCCGG GGGCTGAGAA GGTGACTCCC CAGGAGAACG GCCCTGCCGT CCACAAAGGC 
TCCCCTGAGT TCTCTGCCCA GGAGACCGCC AGCACGTTTT CAGAAGAAGC CCCCACAGCT 
CCTCCAGCAG TGGCTCAGAG CGGCGGCAGT GAGGAGGGGC CCCGGGAGGC TGGCTCCCCT 
GCACAGGAAT TCAACAAGTA CCAAAAGTCA CTCCCTCCCA GATTCCAGCG CCAGCAGCAG 
CAGCAGCAGC AGGAGCAGCT GTACAAGATG CAGCACTGGC AGCCGGTGTA CCCTCCGCCG 
TCCCACCCGC AGCGCACCTT CTACCCGCAC CACCCCCAGA TGCTGGGCTT CGACCCCAGG 
TGGATGATGA TGCCCTCCTA CATGGACCCA CGGATCCCGC CCACTCGGAC CCCAGTGGAT 
TTCTACCCCT CGGCCCTGCA TCCCTCAGGA TTGATGAAAC CCATAATGCC CCCGGATTCC 
CTCAGTGGGA CTGGCTGTCG CTCTGAGGAC CAGAGCTGTG TGCCCCCGCT GCCAGAAAGG 
AAGGTGACCG CCATTGACCC TGCCCCCGTG TGGAGCCCTG AGGGTTACAT GGCGTTGCAG 
AGCAAGGGCT ACTCGCTGCC CCATCCGAAA TCCAGCGACA ATATGGCCAT GGACATGCAT 
GTCAGGAGTG AAAGCTCTTA CTCTGCCTCC CCCGGAAGGT CAGGGGGCGT GAGTGCCCAG 
CGCGAGCTCC TTGAGGAGAG AGGGCAGGAG TACTTGAGCG CTTCTGACAA GAAGGCCCCA 
GCAGACTGTG ACTCCCGTGT CTCCTCTCAG AGAGTAGGCC AGGAGCTTTT GTTTCCACCC 
CAAGAAAATG GGCAGGAAGC AGGCACTCCT GGGAGTCACA CCCCAAACCT CAGGTGCTCC 
CCACTGGAGC CCGACTTTGC CCCCACGGAG AAAAAGCTCG AGTATGGTGA CTGGGATGTG 
AACCACCAGT CAAAGGTTGC CAACCCAGAC GCTGCGGTTG AGAAGGAGGT ACCGCGGGAG 
GAGCCACCCT TCAGCGTCTC CTCCTGGGAG AAGGAAGGGA GCCCGAGCAA GCCGCCGGCT 
CTGGAGCCCG AGTGGACGCC AGAAGCCCGG GGCACTGGCA GCCAGCACCC GGAGCAGACC 
AGCAGGACCC GTAGGTCCGG CCCCATCAAG AAACCTGTCC TGAAGGCCCT CAAGGTGGAG 
GACAAGGAGA AGGAGCTCGA GAAAGTAAAG CCGGAGCTGG CAGAGGGCAG CACCCGCCTG 
GCCCGGGAGC CAGGGCCTGT CCATGAAGTG GCTGAGGACG AGGACCAAGA GAACGACCCC 
GCACTGGCCA ATGCGGCCCC GGCCACCTCG GAGGACCAGG GCTTAGCCCG TGCCAGCTTG 
GGCCGTGAGG CCAGCCGTCC TGAGGAGGAC AACAAGCCGG ATGGGGCCCC GGAGGCCAGA 
CCCTCCAGGG AGGCCAGCCA CACACCCCCG ACCAAGAGGA ATAACTGGAT CTTTATTGAT 
GAGGAGCAAG CCTTTGGGGG CCGGGGGCCA GCCCGGGGCC GAGGCCGCGG GTTCCGAGAG 
TTCACCTTCC GCGGGCGGCC CACCGGTAGT GGCCTGTGTG GCGGGGGCGT CCTTGGGGCA 
CGCGGCCTCT ACAGCGGCAG CCAGAGGAGT AGCCGGGGCC GGGGGCCGCG GGAGTTTGGG 
CCGCCGGATG ACTTCCCCCG GGCCAAGCCC CGGCGGAGGA TTGCCAGTGA GACCCACAGC 
GAGGGCTCTG AGTATGAGGA GCTCCCCAAG CGGCGGCGGC AGCGAGGCAC TGAGCCTGGG 
GATGAGGGTG CGCTCCCGGA GCGGGAGGAG GCCACGGGAA GGAAGGGCGA CTTCAGGGAG 
TCCTGGCGGT CCGGCAGGAT GCACCCTGAG GACCATGGTG GGCCTGAGGC CAAGAACCGA 
GGTCCCCGGG CCTTCGGGCG TGCCCTGCCC CCGCGTCTGA GCAACTGTGC ATATGGCCGG 
AGAACCTTCG CGGCCAAGGA GGTGGCCCCT TGGCCCAGCC GGGGTCCTGG CGCCTCCTGG 
CAGGAGTCTG GCGCCTCTGA CGCATGTGGA GCCCGGCGGC CCTTAGACAG AGACTACATC 
CCAGACGCCT ACAGACGCCC CGACGCCTTG GGCTCCCGGG GCTTCGAGGA TGGCCGCCTG 
GAGGACCGGA GGGCCTTCTT CCAAGATGAC CACGCCGCAG AGTCCGAAAA CGCAGAGAAC 
CGGCCCTTCC GGCGAAGGCG GCCTCCGCGC CAGGACAAGC CGCCCCGCTT CCGGCGCCTC 
CGGCAGGAAC GGGAGGCCCT GGGCCTGTGG GGGCCGGAGG ACGAGCCACC TCTGGCAGCC 
GGCCAGTGGC CAGGCCGTCC TAGACCCTGC CCCGGGGACA GGAGCAGCGC CTCAGGCCGC 
AGGTCCCCTG AGCTCGCCTA TCAGAACTCC TCCGACCACG CCAACGAGGA GTGGGAGACG 
GCCTCCGAGA GCAGTGACTT CAGCGAGAGG CGGGAGCGGC GGGCCGGCTC CGAGCTGGAC 
CCCCAGGCTG ATGGCAGCCC CTCTGGGGTG AGCGCAGGCG AGAAGAAGGA GCTGGCCAAG 
CGGAGCTTCT CGGGCCAGAG ACCCCTGGTG GACAGGCAGA GCCGGAAGCT GGAGCCGGGA 
GGGTTTGGGG AGAAGTCTGT TAGGCCAGGT GGCGGTGACA CTTCTCCCCG CTATGAGAGT 
CATCAGAACG GGACGCCTCT GAAAGCCAAA AGGTCCCCAG ACGAGGCCTT GCCTGGAGGT 
CTTGGCTGCG GCAGTGGGAG CGGCCATTAC ACGCTGGAGC GGGCAGCCCA TGCTGCCTCT 
GACGTCACCG AAGCCTCCTG TGAGAAGGTA GAGAAGGAGG CCAAGCTGGC CGCCCAGAGG 
TCAGGCGAGC AGGGAGAGAC CATGAAACCA TTTGACCTGA ACTACGGACA CGCCATCATT 
GAAAATTGCG GATCCAGCCC TGGGGAGGAG AGTGAGGTGG GCTCTCTGGT GGGCGAAGGC 
TTCATTGAGG TCCTGACCAA GAAGCAGCGC CGCCTGCTGG AGGAGGAGAG GAGAAAGAAG 
GAGCAAGCCG TGCAGGTTCC TGTCAAAGGC CGAAGTCTCT CCTCTCGAAT TCCTCCTCGG 
TTTGCAAAGA AGCAGAACAA CCTGTGCCTG GAGCAGGGTG ATGGGCCTGT GCCCGGCAGC 
AGCCTGGGCA CCGAGATCTG GGAGAGCGGC AGCCAGGCCC TCCCCCTTCA GGCCTCGACC 
AGTGACTCCT GGACCAAAGC TGCCACTGCC TTCACCAGCG CTGAGCCTGG CTCTGCTGAG 
CAGGGCTTTA AGAGCAGCCA GGGAGACAGC GGTGTTGACC TGAGCGCCGA GTCCCGAGAG 
TCCTCAGCGA CCTCCTCGCA GCGCAGCTCT CCATATGGCA CCCTGAAGCC CGAGGAGGCA 
AGCGGGCCCG GCCTGGAGCC CAAGGCCGAT GGCCACAAGG AGCAGACTCA AAAGCAGTCA 
GAGCCAAAGG ATTCAGAACA AGGCTCAGGA CAGAGCAAGG AGCACAGACC AGGACCCATT 
GGCAATGAGC GCTCTCTGAA AAACAGAAAG GGCTCCGAGG GGGCTGAACG GCTGCAGGGG 
GCTGTGGTTC CCCCTGTTAA CGGGGTGGAG ATTCACGTGG ACTCTGTGCT GCCGGTGCCA 
CCCATTGAAT TTGGAGTCAA TCCGAAGGAC TCTGATTTCA GCTTGCAGCC TGGCTCTGCC 
TCTGGTCCTG CGGGGAATCC AGTTGTCAAG CTTCAGGACG CATTGGCCAG TAACGCCGGG 
CTGGCCCCCA GCATCCCCAT CCTGCGGCGG GACCACCACA TCCAGAGGGC CATCGGCCTC 
TCCCACATGT CCTTCCCTAC CGCCGACCTC ACTCTGAAGA TGGAGTCTGC GCGCAAGGCT 
TGGGAAAACT CGCCCAGCTT GCCGGAGCAG AGCTCGCCAG GAGGCGCCGG CTCAGGCCTG 
CAGCCCCCAT CCTCCGGGGG AACCTCCAGC GGGGTCAGCT ATAGCTCCTT TGGCGGCGTC 
TCCATGCCCC CCGTGCCAGT GGCCTCGGTC GCACCTTCTG CATCCCTTCC AGGCAGCCAC 
CTGCCACCTC TCTACCTGGA TGGCCATGTG TTTGCAAGTC AGCCCCGACT GGTTCCTCAG 
ACCATACCTC AGCAGCAGAG TTTCCAACAG GTGATGCCGG CTGCCGCTGC CCAGCAAATC 
CCAATTTCCC TTCACACGTC TCTACAAGCT CAAGCCCAGC TGGGACTGAG GGGCGGGCTG 
CCCGTCTCCC AGTCTCAGGA GATCTTCAGC TCCTTACAGC CCTTCAGGTC TCAGGTGTAC 
ATGCACCCCA GCCTGTCACC GCCCAGCACC ATGATCCTCT CCGGAGGCAC AGCCTTGAAG 
GCCCCCTACT CGGCGTTCCC AGGCATGCAG CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC 
TCGCCCTACC AGCCCATGAG TGGGAGCCAG GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG 
GCGGCTGGGC TTGGCGCCTC CCAGATGTTG GACTCCCAGC TCCCGCAGCT GACGATGCCC 
CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG TACGGCTCCG GGCAGCAGCC CCTGCTCCTG 
CCACAGTCCA TCCAGCTGCC CCAGGCGCAG AGCCTGTCAG TCGGGGCCCC CCGCAGGATC 
CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC AACACCAGCA GAGAGTCCTC TCAGATGGAG 
ATGAAGGGCT TCCACTTTGC CGACAGTAAA CAGAATGTTC CTTCAGGAGG CTCCGTGCCG 
TCACCACAGG CCTACAGGCC TAGCTCTGCT AGCCCCAGTG GGAAGCCCTC TGGATCAGCA 
GTTAACATGG GCTCTGTGCA GGGACACTAC GTTCAACAGG CAAAGCAGCG AGTGGATGAA 
AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG GAGCCACCCT CAGCCGCTTC CCCGCTGAAG 
CGAACTGGAG CAATCAAGCC CCGGGCGGTC AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999604.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855372.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X13, mRNA.

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CloneID	OBa46164
Clone ID Related Accession (Same CDS sequence)	XM_024999604.1 , XM_015465518.1 , XM_010810521.2
Accession Version	XM_024999604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGTATCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855372.1
CDS	311..6166
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPVSF

Target ORF information:

RefSeq Version	XM_024999604.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999604.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGTATCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465518.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321004.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.

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CloneID	OBa46164
Clone ID Related Accession (Same CDS sequence)	XM_024999604.1 , XM_015465518.1 , XM_010810521.2
Accession Version	XM_015465518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGTATCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321004.1
CDS	1412..7267
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPVSF

Target ORF information:

RefSeq Version	XM_015465518.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465518.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGTATCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_010810521.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808823.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.

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CloneID	OBa46164
Clone ID Related Accession (Same CDS sequence)	XM_024999604.1 , XM_015465518.1 , XM_010810521.2
Accession Version	XM_010810521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGTATCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808823.1
CDS	1412..7267
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPVSF

Target ORF information:

RefSeq Version	XM_010810521.2
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810521.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGTATCC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999600.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855368.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.

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CloneID	OBa220952
Clone ID Related Accession (Same CDS sequence)	XM_024999600.1
Accession Version	XM_024999600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855368.1
CDS	311..6997
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999600.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999600.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999602.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855370.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.

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CloneID	OBa220953
Clone ID Related Accession (Same CDS sequence)	XM_024999602.1
Accession Version	XM_024999602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCAAAGC 
AGCGAGTGGA TGAAAAGCCC GGCCTGGGAG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855370.1
CDS	311..6826
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRQSSEWMKSPAWEP

Target ORF information:

RefSeq Version	XM_024999602.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999602.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCAAAGC 
AGCGAGTGGA TGAAAAGCCC GGCCTGGGAG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999606.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855374.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X15, mRNA.

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CloneID	OBa220954
Clone ID Related Accession (Same CDS sequence)	XM_024999606.1
Accession Version	XM_024999606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG CTGCCGCTGC CCAGCAAATC CCAATTTCCC TTCACACGTC TCTACAAGCT 
CAAGCCCAGC TGGGACTGAG GGGCGGGCTG CCCGTCTCCC AGTCTCAGGA GATCTTCAGC 
TCCTTACAGC CCTTCAGGTC TCAGGTGTAC ATGCACCCCA GCCTGTCACC GCCCAGCACC 
ATGATCCTCT CCGGAGGCAC AGCCTTGAAG GCCCCCTACT CGGCGTTCCC AGGCATGCAG 
CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC TCGCCCTACC AGCCCATGAG TGGGAGCCAG 
GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG GCGGCTGGGC TTGGCGCCTC CCAGATGTTG 
GACTCCCAGC TCCCGCAGCT GACGATGCCC CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG 
TACGGCTCCG GGCAGCAGCC CCTGCTCCTG CCACAGTCCA TCCAGCTGCC CCAGGCGCAG 
AGCCTGTCAG TCGGGGCCCC CCGCAGGATC CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC 
AACACCAGCA GAGAGTCCTC TCAGATGGAG ATGAAGGGCT TCCACTTTGC CGACAGTAAA 
CAGAATGTTC CTTCAGGAGG CTCCGTGCCG TCACCACAGG CCTACAGGCC TAGCTCTGCT 
AGCCCCAGTG GGAAGCCCTC TGGATCAGCA GTTAACATGG GCTCTGTGCA GGGACACTAC 
GTTCAACAGG CAAAGCAGCG AGTGGATGAA AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG 
GAGCCACCCT CAGCCGCTTC CCCGCTGAAG CGAACTGGAG CAATCAAGCC CCGGGCGGTC 
AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855374.1
CDS	311..6994
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999606.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999606.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG CTGCCGCTGC CCAGCAAATC CCAATTTCCC TTCACACGTC TCTACAAGCT 
CAAGCCCAGC TGGGACTGAG GGGCGGGCTG CCCGTCTCCC AGTCTCAGGA GATCTTCAGC 
TCCTTACAGC CCTTCAGGTC TCAGGTGTAC ATGCACCCCA GCCTGTCACC GCCCAGCACC 
ATGATCCTCT CCGGAGGCAC AGCCTTGAAG GCCCCCTACT CGGCGTTCCC AGGCATGCAG 
CCCCTGGAGA TGGTGAAGCC CCAGTCCGGC TCGCCCTACC AGCCCATGAG TGGGAGCCAG 
GCCCTGGTCT ATGAGGGCCC GCTCAGCCAG GCGGCTGGGC TTGGCGCCTC CCAGATGTTG 
GACTCCCAGC TCCCGCAGCT GACGATGCCC CTGCCTGGCT CCCAGCTGCC TCTGCCCCGG 
TACGGCTCCG GGCAGCAGCC CCTGCTCCTG CCACAGTCCA TCCAGCTGCC CCAGGCGCAG 
AGCCTGTCAG TCGGGGCCCC CCGCAGGATC CTTGCCCCCG GGTCCCAGCC TTCGGTCCTC 
AACACCAGCA GAGAGTCCTC TCAGATGGAG ATGAAGGGCT TCCACTTTGC CGACAGTAAA 
CAGAATGTTC CTTCAGGAGG CTCCGTGCCG TCACCACAGG CCTACAGGCC TAGCTCTGCT 
AGCCCCAGTG GGAAGCCCTC TGGATCAGCA GTTAACATGG GCTCTGTGCA GGGACACTAC 
GTTCAACAGG CAAAGCAGCG AGTGGATGAA AAGCCCGGCC TGGGAGCCAT GAAGCTGCAG 
GAGCCACCCT CAGCCGCTTC CCCGCTGAAG CGAACTGGAG CAATCAAGCC CCGGGCGGTC 
AAGGTGGAGG AGAGCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213441.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213498.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.

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CloneID	OBa24515
Clone ID Related Accession (Same CDS sequence)	XM_005213441.3 , XM_015465510.1 , XM_024999593.1 , XM_024999594.1
Accession Version	XM_005213441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213498.1
CDS	1411..8109
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_005213441.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213441.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465510.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320996.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.

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CloneID	OBa24515
Clone ID Related Accession (Same CDS sequence)	XM_005213441.3 , XM_015465510.1 , XM_024999593.1 , XM_024999594.1
Accession Version	XM_015465510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320996.1
CDS	1411..8109
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465510.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465510.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999593.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855361.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.

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CloneID	OBa24515
Clone ID Related Accession (Same CDS sequence)	XM_005213441.3 , XM_015465510.1 , XM_024999593.1 , XM_024999594.1
Accession Version	XM_024999593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855361.1
CDS	311..7009
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999593.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999593.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999594.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855362.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.

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CloneID	OBa24515
Clone ID Related Accession (Same CDS sequence)	XM_005213441.3 , XM_015465510.1 , XM_024999593.1 , XM_024999594.1
Accession Version	XM_024999594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855362.1
CDS	138..6836
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999594.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999594.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCCTAGCT CTGCTAGCCC CAGTGGGAAG CCCTCTGGAT CAGCAGTTAA CATGGGCTCT 
GTGCAGGGAC ACTACGTTCA ACAGGCAAAG CAGCGAGTGG ATGAAAAGCC CGGCCTGGGA 
GCCATGAAGC TGCAGGAGCC ACCCTCAGCC GCTTCCCCGC TGAAGCGAAC TGGAGCAATC 
AAGCCCCGGG CGGTCAAGGT GGAGGAGAGC AAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855363.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.

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CloneID	OBa24516
Clone ID Related Accession (Same CDS sequence)	XM_024999595.1 , XM_015465511.1 , XM_005213442.3
Accession Version	XM_024999595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855363.1
CDS	311..7006
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999595.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465511.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320997.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.

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CloneID	OBa24516
Clone ID Related Accession (Same CDS sequence)	XM_024999595.1 , XM_015465511.1 , XM_005213442.3
Accession Version	XM_015465511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320997.1
CDS	1411..8106
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465511.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465511.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213442.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213499.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.

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CloneID	OBa24516
Clone ID Related Accession (Same CDS sequence)	XM_024999595.1 , XM_015465511.1 , XM_005213442.3
Accession Version	XM_005213442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213499.1
CDS	1411..8106
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_005213442.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213442.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465512.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320998.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.

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CloneID	OBa24517
Clone ID Related Accession (Same CDS sequence)	XM_015465512.1 , XM_005213443.3 , XM_024999596.1
Accession Version	XM_015465512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320998.1
CDS	1412..8107
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465512.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465512.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213443.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213500.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.

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CloneID	OBa24517
Clone ID Related Accession (Same CDS sequence)	XM_015465512.1 , XM_005213443.3 , XM_024999596.1
Accession Version	XM_005213443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213500.1
CDS	1412..8107
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_005213443.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213443.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999596.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855364.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.

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CloneID	OBa24517
Clone ID Related Accession (Same CDS sequence)	XM_015465512.1 , XM_005213443.3 , XM_024999596.1
Accession Version	XM_024999596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855364.1
CDS	311..7006
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999596.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999596.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGTGATGCC GGCTGCCGCT GCCCAGCAAA TCCCAATTTC CCTTCACACG 
TCTCTACAAG CTCAAGCCCA GCTGGGACTG AGGGGCGGGC TGCCCGTCTC CCAGTCTCAG 
GAGATCTTCA GCTCCTTACA GCCCTTCAGG TCTCAGGTGT ACATGCACCC CAGCCTGTCA 
CCGCCCAGCA CCATGATCCT CTCCGGAGGC ACAGCCTTGA AGGCCCCCTA CTCGGCGTTC 
CCAGGCATGC AGCCCCTGGA GATGGTGAAG CCCCAGTCCG GCTCGCCCTA CCAGCCCATG 
AGTGGGAGCC AGGCCCTGGT CTATGAGGGC CCGCTCAGCC AGGCGGCTGG GCTTGGCGCC 
TCCCAGATGT TGGACTCCCA GCTCCCGCAG CTGACGATGC CCCTGCCTGG CTCCCAGCTG 
CCTCTGCCCC GGTACGGCTC CGGGCAGCAG CCCCTGCTCC TGCCACAGTC CATCCAGCTG 
CCCCAGGCGC AGAGCCTGTC AGTCGGGGCC CCCCGCAGGA TCCTTGCCCC CGGGTCCCAG 
CCTTCGGTCC TCAACACCAG CAGAGAGTCC TCTCAGATGG AGATGAAGGG CTTCCACTTT 
GCCGACAGTA AACAGAATGT TCCTTCAGGA GGCTCCGTGC CGTCACCACA GGCCTACAGG 
CCTAGCTCTG CTAGCCCCAG TGGGAAGCCC TCTGGATCAG CAGTTAACAT GGGCTCTGTG 
CAGGGACACT ACGTTCAACA GGCAAAGCAG CGAGTGGATG AAAAGCCCGG CCTGGGAGCC 
ATGAAGCTGC AGGAGCCACC CTCAGCCGCT TCCCCGCTGA AGCGAACTGG AGCAATCAAG 
CCCCGGGCGG TCAAGGTGGA GGAGAGCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465513.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320999.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.

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CloneID	OBa24518
Clone ID Related Accession (Same CDS sequence)	XM_015465513.1 , XM_024999597.1 , XM_005213444.3
Accession Version	XM_015465513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GTTGGGACCC CAGGAGGCAG CGGCAGTTAT CCTTGAGCTC TGCAGACAGT 
GCTGATGCCA AGCGCACCCA AGAGGAAGGA AAAGACTGGA GTGAAACAGG AGGCGTGACC 
CGTGTCGTCC GGAAGGTGCC GGAACCTCAG CCGCCTTCCA GGAAGCTGCA CAGCTGGGCG 
TCAGGCCCTG ACTACCAGCA GAAATCCTCC ATGGGCACTG TATTTCGGCA GCAGTCTGCC 
GAGGACAAAG AGGACAAGCC CCCACCGCGG CAGAAGTTTG TCCAGTCTGA GATGTCCGAG 
GCTGTGGAGC GAGCCCGGAA GCGCCGGGAG GAGGAGGAGC GCCGTGCGCG CGAGGAAAGG 
CTGGCTGCCT GTGCTGCCAA ACTCAAGCAG CTTGACCAGA AGTGCAAGCA GGCTCAGCGG 
GCCAGCGAGG CCCAGAAGCA GGCGGAGAAG GAAGCTCCCC GCTCTCCGGG GGCTGAGAAG 
GTGACTCCCC AGGAGAACGG CCCTGCCGTC CACAAAGGCT CCCCTGAGTT CTCTGCCCAG 
GAGACCGCCA GCACGTTTTC AGAAGAAGCC CCCACAGCTC CTCCAGCAGT GGCTCAGAGC 
GGCGGCAGTG AGGAGGGGCC CCGGGAGGCT GGCTCCCCTG CACAGGAATT CAACAAGTAC 
CAAAAGTCAC TCCCTCCCAG ATTCCAGCGC CAGCAGCAGC AGCAGCAGCA GGAGCAGCTG 
TACAAGATGC AGCACTGGCA GCCGGTGTAC CCTCCGCCGT CCCACCCGCA GCGCACCTTC 
TACCCGCACC ACCCCCAGAT GCTGGGCTTC GACCCCAGGT GGATGATGAT GCCCTCCTAC 
ATGGACCCAC GGATCCCGCC CACTCGGACC CCAGTGGATT TCTACCCCTC GGCCCTGCAT 
CCCTCAGGAT TGATGAAACC CATAATGCCC CCGGATTCCC TCAGTGGGAC TGGCTGTCGC 
TCTGAGGACC AGAGCTGTGT GCCCCCGCTG CCAGAAAGGA AGGTGACCGC CATTGACCCT 
GCCCCCGTGT GGAGCCCTGA GGGTTACATG GCGTTGCAGA GCAAGGGCTA CTCGCTGCCC 
CATCCGAAAT CCAGCGACAA TATGGCCATG GACATGCATG TCAGGAGTGA AAGCTCTTAC 
TCTGCCTCCC CCGGAAGGTC AGGGGGCGTG AGTGCCCAGC GCGAGCTCCT TGAGGAGAGA 
GGGCAGGAGT ACTTGAGCGC TTCTGACAAG AAGGCCCCAG CAGACTGTGA CTCCCGTGTC 
TCCTCTCAGA GAGTAGGCCA GGAGCTTTTG TTTCCACCCC AAGAAAATGG GCAGGAAGCA 
GGCACTCCTG GGAGTCACAC CCCAAACCTC AGGTGCTCCC CACTGGAGCC CGACTTTGCC 
CCCACGGAGA AAAAGCTCGA GTATGGTGAC TGGGATGTGA ACCACCAGTC AAAGGTTGCC 
AACCCAGACG CTGCGGTTGA GAAGGAGGTA CCGCGGGAGG AGCCACCCTT CAGCGTCTCC 
TCCTGGGAGA AGGAAGGGAG CCCGAGCAAG CCGCCGGCTC TGGAGCCCGA GTGGACGCCA 
GAAGCCCGGG GCACTGGCAG CCAGCACCCG GAGCAGACCA GCAGGACCCG TAGGTCCGGC 
CCCATCAAGA AACCTGTCCT GAAGGCCCTC AAGGTGGAGG ACAAGGAGAA GGAGCTCGAG 
AAAGTAAAGC CGGAGCTGGC AGAGGGCAGC ACCCGCCTGG CCCGGGAGCC AGGGCCTGTC 
CATGAAGTGG CTGAGGACGA GGACCAAGAG AACGACCCCG CACTGGCCAA TGCGGCCCCG 
GCCACCTCGG AGGACCAGGG CTTAGCCCGT GCCAGCTTGG GCCGTGAGGC CAGCCGTCCT 
GAGGAGGACA ACAAGCCGGA TGGGGCCCCG GAGGCCAGAC CCTCCAGGGA GGCCAGCCAC 
ACACCCCCGA CCAAGAGGAA TAACTGGATC TTTATTGATG AGGAGCAAGC CTTTGGGGGC 
CGGGGGCCAG CCCGGGGCCG AGGCCGCGGG TTCCGAGAGT TCACCTTCCG CGGGCGGCCC 
ACCGGTAGTG GCCTGTGTGG CGGGGGCGTC CTTGGGGCAC GCGGCCTCTA CAGCGGCAGC 
CAGAGGAGTA GCCGGGGCCG GGGGCCGCGG GAGTTTGGGC CGCCGGATGA CTTCCCCCGG 
GCCAAGCCCC GGCGGAGGAT TGCCAGTGAG ACCCACAGCG AGGGCTCTGA GTATGAGGAG 
CTCCCCAAGC GGCGGCGGCA GCGAGGCACT GAGCCTGGGG ATGAGGGTGC GCTCCCGGAG 
CGGGAGGAGG CCACGGGAAG GAAGGGCGAC TTCAGGGAGT CCTGGCGGTC CGGCAGGATG 
CACCCTGAGG ACCATGGTGG GCCTGAGGCC AAGAACCGAG GTCCCCGGGC CTTCGGGCGT 
GCCCTGCCCC CGCGTCTGAG CAACTGTGCA TATGGCCGGA GAACCTTCGC GGCCAAGGAG 
GTGGCCCCTT GGCCCAGCCG GGGTCCTGGC GCCTCCTGGC AGGAGTCTGG CGCCTCTGAC 
GCATGTGGAG CCCGGCGGCC CTTAGACAGA GACTACATCC CAGACGCCTA CAGACGCCCC 
GACGCCTTGG GCTCCCGGGG CTTCGAGGAT GGCCGCCTGG AGGACCGGAG GGCCTTCTTC 
CAAGATGACC ACGCCGCAGA GTCCGAAAAC GCAGAGAACC GGCCCTTCCG GCGAAGGCGG 
CCTCCGCGCC AGGACAAGCC GCCCCGCTTC CGGCGCCTCC GGCAGGAACG GGAGGCCCTG 
GGCCTGTGGG GGCCGGAGGA CGAGCCACCT CTGGCAGCCG GCCAGTGGCC AGGCCGTCCT 
AGACCCTGCC CCGGGGACAG GAGCAGCGCC TCAGGCCGCA GGTCCCCTGA GCTCGCCTAT 
CAGAACTCCT CCGACCACGC CAACGAGGAG TGGGAGACGG CCTCCGAGAG CAGTGACTTC 
AGCGAGAGGC GGGAGCGGCG GGCCGGCTCC GAGCTGGACC CCCAGGCTGA TGGCAGCCCC 
TCTGGGGTGA GCGCAGGCGA GAAGAAGGAG CTGGCCAAGC GGAGCTTCTC GGGCCAGAGA 
CCCCTGGTGG ACAGGCAGAG CCGGAAGCTG GAGCCGGGAG GGTTTGGGGA GAAGTCTGTT 
AGGCCAGGTG GCGGTGACAC TTCTCCCCGC TATGAGAGTC ATCAGAACGG GACGCCTCTG 
AAAGCCAAAA GGTCCCCAGA CGAGGCCTTG CCTGGAGGTC TTGGCTGCGG CAGTGGGAGC 
GGCCATTACA CGCTGGAGCG GGCAGCCCAT GCTGCCTCTG ACGTCACCGA AGCCTCCTGT 
GAGAAGGTAG AGAAGGAGGC CAAGCTGGCC GCCCAGAGGT CAGGCGAGCA GGGAGAGACC 
ATGAAACCAT TTGACCTGAA CTACGGACAC GCCATCATTG AAAATTGCGG ATCCAGCCCT 
GGGGAGGAGA GTGAGGTGGG CTCTCTGGTG GGCGAAGGCT TCATTGAGGT CCTGACCAAG 
AAGCAGCGCC GCCTGCTGGA GGAGGAGAGG AGAAAGAAGG AGCAAGCCGT GCAGGTTCCT 
GTCAAAGGCC GAAGTCTCTC CTCTCGAATT CCTCCTCGGT TTGCAAAGAA GCAGAACAAC 
CTGTGCCTGG AGCAGGGTGA TGGGCCTGTG CCCGGCAGCA GCCTGGGCAC CGAGATCTGG 
GAGAGCGGCA GCCAGGCCCT CCCCCTTCAG GCCTCGACCA GTGACTCCTG GACCAAAGCT 
GCCACTGCCT TCACCAGCGC TGAGCCTGGC TCTGCTGAGC AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG TGATGCCGGC TGCCGCTGCC CAGCAAATCC CAATTTCCCT TCACACGTCT 
CTACAAGCTC AAGCCCAGCT GGGACTGAGG GGCGGGCTGC CCGTCTCCCA GTCTCAGGAG 
ATCTTCAGCT CCTTACAGCC CTTCAGGTCT CAGGTGTACA TGCACCCCAG CCTGTCACCG 
CCCAGCACCA TGATCCTCTC CGGAGGCACA GCCTTGAAGG CCCCCTACTC GGCGTTCCCA 
GGCATGCAGC CCCTGGAGAT GGTGAAGCCC CAGTCCGGCT CGCCCTACCA GCCCATGAGT 
GGGAGCCAGG CCCTGGTCTA TGAGGGCCCG CTCAGCCAGG CGGCTGGGCT TGGCGCCTCC 
CAGATGTTGG ACTCCCAGCT CCCGCAGCTG ACGATGCCCC TGCCTGGCTC CCAGCTGCCT 
CTGCCCCGGT ACGGCTCCGG GCAGCAGCCC CTGCTCCTGC CACAGTCCAT CCAGCTGCCC 
CAGGCGCAGA GCCTGTCAGT CGGGGCCCCC CGCAGGATCC TTGCCCCCGG GTCCCAGCCT 
TCGGTCCTCA ACACCAGCAG AGAGTCCTCT CAGATGGAGA TGAAGGGCTT CCACTTTGCC 
GACAGTAAAC AGAATGTTCC TTCAGGAGGC TCCGTGCCGT CACCACAGGC CTACAGGCCT 
AGCTCTGCTA GCCCCAGTGG GAAGCCCTCT GGATCAGCAG TTAACATGGG CTCTGTGCAG 
GGACACTACG TTCAACAGGC AAAGCAGCGA GTGGATGAAA AGCCCGGCCT GGGAGCCATG 
AAGCTGCAGG AGCCACCCTC AGCCGCTTCC CCGCTGAAGC GAACTGGAGC AATCAAGCCC 
CGGGCGGTCA AGGTGGAGGA GAGCAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320999.1
CDS	1412..8104
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKCWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465513.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465513.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GTTGGGACCC CAGGAGGCAG CGGCAGTTAT CCTTGAGCTC TGCAGACAGT 
GCTGATGCCA AGCGCACCCA AGAGGAAGGA AAAGACTGGA GTGAAACAGG AGGCGTGACC 
CGTGTCGTCC GGAAGGTGCC GGAACCTCAG CCGCCTTCCA GGAAGCTGCA CAGCTGGGCG 
TCAGGCCCTG ACTACCAGCA GAAATCCTCC ATGGGCACTG TATTTCGGCA GCAGTCTGCC 
GAGGACAAAG AGGACAAGCC CCCACCGCGG CAGAAGTTTG TCCAGTCTGA GATGTCCGAG 
GCTGTGGAGC GAGCCCGGAA GCGCCGGGAG GAGGAGGAGC GCCGTGCGCG CGAGGAAAGG 
CTGGCTGCCT GTGCTGCCAA ACTCAAGCAG CTTGACCAGA AGTGCAAGCA GGCTCAGCGG 
GCCAGCGAGG CCCAGAAGCA GGCGGAGAAG GAAGCTCCCC GCTCTCCGGG GGCTGAGAAG 
GTGACTCCCC AGGAGAACGG CCCTGCCGTC CACAAAGGCT CCCCTGAGTT CTCTGCCCAG 
GAGACCGCCA GCACGTTTTC AGAAGAAGCC CCCACAGCTC CTCCAGCAGT GGCTCAGAGC 
GGCGGCAGTG AGGAGGGGCC CCGGGAGGCT GGCTCCCCTG CACAGGAATT CAACAAGTAC 
CAAAAGTCAC TCCCTCCCAG ATTCCAGCGC CAGCAGCAGC AGCAGCAGCA GGAGCAGCTG 
TACAAGATGC AGCACTGGCA GCCGGTGTAC CCTCCGCCGT CCCACCCGCA GCGCACCTTC 
TACCCGCACC ACCCCCAGAT GCTGGGCTTC GACCCCAGGT GGATGATGAT GCCCTCCTAC 
ATGGACCCAC GGATCCCGCC CACTCGGACC CCAGTGGATT TCTACCCCTC GGCCCTGCAT 
CCCTCAGGAT TGATGAAACC CATAATGCCC CCGGATTCCC TCAGTGGGAC TGGCTGTCGC 
TCTGAGGACC AGAGCTGTGT GCCCCCGCTG CCAGAAAGGA AGGTGACCGC CATTGACCCT 
GCCCCCGTGT GGAGCCCTGA GGGTTACATG GCGTTGCAGA GCAAGGGCTA CTCGCTGCCC 
CATCCGAAAT CCAGCGACAA TATGGCCATG GACATGCATG TCAGGAGTGA AAGCTCTTAC 
TCTGCCTCCC CCGGAAGGTC AGGGGGCGTG AGTGCCCAGC GCGAGCTCCT TGAGGAGAGA 
GGGCAGGAGT ACTTGAGCGC TTCTGACAAG AAGGCCCCAG CAGACTGTGA CTCCCGTGTC 
TCCTCTCAGA GAGTAGGCCA GGAGCTTTTG TTTCCACCCC AAGAAAATGG GCAGGAAGCA 
GGCACTCCTG GGAGTCACAC CCCAAACCTC AGGTGCTCCC CACTGGAGCC CGACTTTGCC 
CCCACGGAGA AAAAGCTCGA GTATGGTGAC TGGGATGTGA ACCACCAGTC AAAGGTTGCC 
AACCCAGACG CTGCGGTTGA GAAGGAGGTA CCGCGGGAGG AGCCACCCTT CAGCGTCTCC 
TCCTGGGAGA AGGAAGGGAG CCCGAGCAAG CCGCCGGCTC TGGAGCCCGA GTGGACGCCA 
GAAGCCCGGG GCACTGGCAG CCAGCACCCG GAGCAGACCA GCAGGACCCG TAGGTCCGGC 
CCCATCAAGA AACCTGTCCT GAAGGCCCTC AAGGTGGAGG ACAAGGAGAA GGAGCTCGAG 
AAAGTAAAGC CGGAGCTGGC AGAGGGCAGC ACCCGCCTGG CCCGGGAGCC AGGGCCTGTC 
CATGAAGTGG CTGAGGACGA GGACCAAGAG AACGACCCCG CACTGGCCAA TGCGGCCCCG 
GCCACCTCGG AGGACCAGGG CTTAGCCCGT GCCAGCTTGG GCCGTGAGGC CAGCCGTCCT 
GAGGAGGACA ACAAGCCGGA TGGGGCCCCG GAGGCCAGAC CCTCCAGGGA GGCCAGCCAC 
ACACCCCCGA CCAAGAGGAA TAACTGGATC TTTATTGATG AGGAGCAAGC CTTTGGGGGC 
CGGGGGCCAG CCCGGGGCCG AGGCCGCGGG TTCCGAGAGT TCACCTTCCG CGGGCGGCCC 
ACCGGTAGTG GCCTGTGTGG CGGGGGCGTC CTTGGGGCAC GCGGCCTCTA CAGCGGCAGC 
CAGAGGAGTA GCCGGGGCCG GGGGCCGCGG GAGTTTGGGC CGCCGGATGA CTTCCCCCGG 
GCCAAGCCCC GGCGGAGGAT TGCCAGTGAG ACCCACAGCG AGGGCTCTGA GTATGAGGAG 
CTCCCCAAGC GGCGGCGGCA GCGAGGCACT GAGCCTGGGG ATGAGGGTGC GCTCCCGGAG 
CGGGAGGAGG CCACGGGAAG GAAGGGCGAC TTCAGGGAGT CCTGGCGGTC CGGCAGGATG 
CACCCTGAGG ACCATGGTGG GCCTGAGGCC AAGAACCGAG GTCCCCGGGC CTTCGGGCGT 
GCCCTGCCCC CGCGTCTGAG CAACTGTGCA TATGGCCGGA GAACCTTCGC GGCCAAGGAG 
GTGGCCCCTT GGCCCAGCCG GGGTCCTGGC GCCTCCTGGC AGGAGTCTGG CGCCTCTGAC 
GCATGTGGAG CCCGGCGGCC CTTAGACAGA GACTACATCC CAGACGCCTA CAGACGCCCC 
GACGCCTTGG GCTCCCGGGG CTTCGAGGAT GGCCGCCTGG AGGACCGGAG GGCCTTCTTC 
CAAGATGACC ACGCCGCAGA GTCCGAAAAC GCAGAGAACC GGCCCTTCCG GCGAAGGCGG 
CCTCCGCGCC AGGACAAGCC GCCCCGCTTC CGGCGCCTCC GGCAGGAACG GGAGGCCCTG 
GGCCTGTGGG GGCCGGAGGA CGAGCCACCT CTGGCAGCCG GCCAGTGGCC AGGCCGTCCT 
AGACCCTGCC CCGGGGACAG GAGCAGCGCC TCAGGCCGCA GGTCCCCTGA GCTCGCCTAT 
CAGAACTCCT CCGACCACGC CAACGAGGAG TGGGAGACGG CCTCCGAGAG CAGTGACTTC 
AGCGAGAGGC GGGAGCGGCG GGCCGGCTCC GAGCTGGACC CCCAGGCTGA TGGCAGCCCC 
TCTGGGGTGA GCGCAGGCGA GAAGAAGGAG CTGGCCAAGC GGAGCTTCTC GGGCCAGAGA 
CCCCTGGTGG ACAGGCAGAG CCGGAAGCTG GAGCCGGGAG GGTTTGGGGA GAAGTCTGTT 
AGGCCAGGTG GCGGTGACAC TTCTCCCCGC TATGAGAGTC ATCAGAACGG GACGCCTCTG 
AAAGCCAAAA GGTCCCCAGA CGAGGCCTTG CCTGGAGGTC TTGGCTGCGG CAGTGGGAGC 
GGCCATTACA CGCTGGAGCG GGCAGCCCAT GCTGCCTCTG ACGTCACCGA AGCCTCCTGT 
GAGAAGGTAG AGAAGGAGGC CAAGCTGGCC GCCCAGAGGT CAGGCGAGCA GGGAGAGACC 
ATGAAACCAT TTGACCTGAA CTACGGACAC GCCATCATTG AAAATTGCGG ATCCAGCCCT 
GGGGAGGAGA GTGAGGTGGG CTCTCTGGTG GGCGAAGGCT TCATTGAGGT CCTGACCAAG 
AAGCAGCGCC GCCTGCTGGA GGAGGAGAGG AGAAAGAAGG AGCAAGCCGT GCAGGTTCCT 
GTCAAAGGCC GAAGTCTCTC CTCTCGAATT CCTCCTCGGT TTGCAAAGAA GCAGAACAAC 
CTGTGCCTGG AGCAGGGTGA TGGGCCTGTG CCCGGCAGCA GCCTGGGCAC CGAGATCTGG 
GAGAGCGGCA GCCAGGCCCT CCCCCTTCAG GCCTCGACCA GTGACTCCTG GACCAAAGCT 
GCCACTGCCT TCACCAGCGC TGAGCCTGGC TCTGCTGAGC AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG TGATGCCGGC TGCCGCTGCC CAGCAAATCC CAATTTCCCT TCACACGTCT 
CTACAAGCTC AAGCCCAGCT GGGACTGAGG GGCGGGCTGC CCGTCTCCCA GTCTCAGGAG 
ATCTTCAGCT CCTTACAGCC CTTCAGGTCT CAGGTGTACA TGCACCCCAG CCTGTCACCG 
CCCAGCACCA TGATCCTCTC CGGAGGCACA GCCTTGAAGG CCCCCTACTC GGCGTTCCCA 
GGCATGCAGC CCCTGGAGAT GGTGAAGCCC CAGTCCGGCT CGCCCTACCA GCCCATGAGT 
GGGAGCCAGG CCCTGGTCTA TGAGGGCCCG CTCAGCCAGG CGGCTGGGCT TGGCGCCTCC 
CAGATGTTGG ACTCCCAGCT CCCGCAGCTG ACGATGCCCC TGCCTGGCTC CCAGCTGCCT 
CTGCCCCGGT ACGGCTCCGG GCAGCAGCCC CTGCTCCTGC CACAGTCCAT CCAGCTGCCC 
CAGGCGCAGA GCCTGTCAGT CGGGGCCCCC CGCAGGATCC TTGCCCCCGG GTCCCAGCCT 
TCGGTCCTCA ACACCAGCAG AGAGTCCTCT CAGATGGAGA TGAAGGGCTT CCACTTTGCC 
GACAGTAAAC AGAATGTTCC TTCAGGAGGC TCCGTGCCGT CACCACAGGC CTACAGGCCT 
AGCTCTGCTA GCCCCAGTGG GAAGCCCTCT GGATCAGCAG TTAACATGGG CTCTGTGCAG 
GGACACTACG TTCAACAGGC AAAGCAGCGA GTGGATGAAA AGCCCGGCCT GGGAGCCATG 
AAGCTGCAGG AGCCACCCTC AGCCGCTTCC CCGCTGAAGC GAACTGGAGC AATCAAGCCC 
CGGGCGGTCA AGGTGGAGGA GAGCAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999597.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855365.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.

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CloneID	OBa24518
Clone ID Related Accession (Same CDS sequence)	XM_015465513.1 , XM_024999597.1 , XM_005213444.3
Accession Version	XM_024999597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GTTGGGACCC CAGGAGGCAG CGGCAGTTAT CCTTGAGCTC TGCAGACAGT 
GCTGATGCCA AGCGCACCCA AGAGGAAGGA AAAGACTGGA GTGAAACAGG AGGCGTGACC 
CGTGTCGTCC GGAAGGTGCC GGAACCTCAG CCGCCTTCCA GGAAGCTGCA CAGCTGGGCG 
TCAGGCCCTG ACTACCAGCA GAAATCCTCC ATGGGCACTG TATTTCGGCA GCAGTCTGCC 
GAGGACAAAG AGGACAAGCC CCCACCGCGG CAGAAGTTTG TCCAGTCTGA GATGTCCGAG 
GCTGTGGAGC GAGCCCGGAA GCGCCGGGAG GAGGAGGAGC GCCGTGCGCG CGAGGAAAGG 
CTGGCTGCCT GTGCTGCCAA ACTCAAGCAG CTTGACCAGA AGTGCAAGCA GGCTCAGCGG 
GCCAGCGAGG CCCAGAAGCA GGCGGAGAAG GAAGCTCCCC GCTCTCCGGG GGCTGAGAAG 
GTGACTCCCC AGGAGAACGG CCCTGCCGTC CACAAAGGCT CCCCTGAGTT CTCTGCCCAG 
GAGACCGCCA GCACGTTTTC AGAAGAAGCC CCCACAGCTC CTCCAGCAGT GGCTCAGAGC 
GGCGGCAGTG AGGAGGGGCC CCGGGAGGCT GGCTCCCCTG CACAGGAATT CAACAAGTAC 
CAAAAGTCAC TCCCTCCCAG ATTCCAGCGC CAGCAGCAGC AGCAGCAGCA GGAGCAGCTG 
TACAAGATGC AGCACTGGCA GCCGGTGTAC CCTCCGCCGT CCCACCCGCA GCGCACCTTC 
TACCCGCACC ACCCCCAGAT GCTGGGCTTC GACCCCAGGT GGATGATGAT GCCCTCCTAC 
ATGGACCCAC GGATCCCGCC CACTCGGACC CCAGTGGATT TCTACCCCTC GGCCCTGCAT 
CCCTCAGGAT TGATGAAACC CATAATGCCC CCGGATTCCC TCAGTGGGAC TGGCTGTCGC 
TCTGAGGACC AGAGCTGTGT GCCCCCGCTG CCAGAAAGGA AGGTGACCGC CATTGACCCT 
GCCCCCGTGT GGAGCCCTGA GGGTTACATG GCGTTGCAGA GCAAGGGCTA CTCGCTGCCC 
CATCCGAAAT CCAGCGACAA TATGGCCATG GACATGCATG TCAGGAGTGA AAGCTCTTAC 
TCTGCCTCCC CCGGAAGGTC AGGGGGCGTG AGTGCCCAGC GCGAGCTCCT TGAGGAGAGA 
GGGCAGGAGT ACTTGAGCGC TTCTGACAAG AAGGCCCCAG CAGACTGTGA CTCCCGTGTC 
TCCTCTCAGA GAGTAGGCCA GGAGCTTTTG TTTCCACCCC AAGAAAATGG GCAGGAAGCA 
GGCACTCCTG GGAGTCACAC CCCAAACCTC AGGTGCTCCC CACTGGAGCC CGACTTTGCC 
CCCACGGAGA AAAAGCTCGA GTATGGTGAC TGGGATGTGA ACCACCAGTC AAAGGTTGCC 
AACCCAGACG CTGCGGTTGA GAAGGAGGTA CCGCGGGAGG AGCCACCCTT CAGCGTCTCC 
TCCTGGGAGA AGGAAGGGAG CCCGAGCAAG CCGCCGGCTC TGGAGCCCGA GTGGACGCCA 
GAAGCCCGGG GCACTGGCAG CCAGCACCCG GAGCAGACCA GCAGGACCCG TAGGTCCGGC 
CCCATCAAGA AACCTGTCCT GAAGGCCCTC AAGGTGGAGG ACAAGGAGAA GGAGCTCGAG 
AAAGTAAAGC CGGAGCTGGC AGAGGGCAGC ACCCGCCTGG CCCGGGAGCC AGGGCCTGTC 
CATGAAGTGG CTGAGGACGA GGACCAAGAG AACGACCCCG CACTGGCCAA TGCGGCCCCG 
GCCACCTCGG AGGACCAGGG CTTAGCCCGT GCCAGCTTGG GCCGTGAGGC CAGCCGTCCT 
GAGGAGGACA ACAAGCCGGA TGGGGCCCCG GAGGCCAGAC CCTCCAGGGA GGCCAGCCAC 
ACACCCCCGA CCAAGAGGAA TAACTGGATC TTTATTGATG AGGAGCAAGC CTTTGGGGGC 
CGGGGGCCAG CCCGGGGCCG AGGCCGCGGG TTCCGAGAGT TCACCTTCCG CGGGCGGCCC 
ACCGGTAGTG GCCTGTGTGG CGGGGGCGTC CTTGGGGCAC GCGGCCTCTA CAGCGGCAGC 
CAGAGGAGTA GCCGGGGCCG GGGGCCGCGG GAGTTTGGGC CGCCGGATGA CTTCCCCCGG 
GCCAAGCCCC GGCGGAGGAT TGCCAGTGAG ACCCACAGCG AGGGCTCTGA GTATGAGGAG 
CTCCCCAAGC GGCGGCGGCA GCGAGGCACT GAGCCTGGGG ATGAGGGTGC GCTCCCGGAG 
CGGGAGGAGG CCACGGGAAG GAAGGGCGAC TTCAGGGAGT CCTGGCGGTC CGGCAGGATG 
CACCCTGAGG ACCATGGTGG GCCTGAGGCC AAGAACCGAG GTCCCCGGGC CTTCGGGCGT 
GCCCTGCCCC CGCGTCTGAG CAACTGTGCA TATGGCCGGA GAACCTTCGC GGCCAAGGAG 
GTGGCCCCTT GGCCCAGCCG GGGTCCTGGC GCCTCCTGGC AGGAGTCTGG CGCCTCTGAC 
GCATGTGGAG CCCGGCGGCC CTTAGACAGA GACTACATCC CAGACGCCTA CAGACGCCCC 
GACGCCTTGG GCTCCCGGGG CTTCGAGGAT GGCCGCCTGG AGGACCGGAG GGCCTTCTTC 
CAAGATGACC ACGCCGCAGA GTCCGAAAAC GCAGAGAACC GGCCCTTCCG GCGAAGGCGG 
CCTCCGCGCC AGGACAAGCC GCCCCGCTTC CGGCGCCTCC GGCAGGAACG GGAGGCCCTG 
GGCCTGTGGG GGCCGGAGGA CGAGCCACCT CTGGCAGCCG GCCAGTGGCC AGGCCGTCCT 
AGACCCTGCC CCGGGGACAG GAGCAGCGCC TCAGGCCGCA GGTCCCCTGA GCTCGCCTAT 
CAGAACTCCT CCGACCACGC CAACGAGGAG TGGGAGACGG CCTCCGAGAG CAGTGACTTC 
AGCGAGAGGC GGGAGCGGCG GGCCGGCTCC GAGCTGGACC CCCAGGCTGA TGGCAGCCCC 
TCTGGGGTGA GCGCAGGCGA GAAGAAGGAG CTGGCCAAGC GGAGCTTCTC GGGCCAGAGA 
CCCCTGGTGG ACAGGCAGAG CCGGAAGCTG GAGCCGGGAG GGTTTGGGGA GAAGTCTGTT 
AGGCCAGGTG GCGGTGACAC TTCTCCCCGC TATGAGAGTC ATCAGAACGG GACGCCTCTG 
AAAGCCAAAA GGTCCCCAGA CGAGGCCTTG CCTGGAGGTC TTGGCTGCGG CAGTGGGAGC 
GGCCATTACA CGCTGGAGCG GGCAGCCCAT GCTGCCTCTG ACGTCACCGA AGCCTCCTGT 
GAGAAGGTAG AGAAGGAGGC CAAGCTGGCC GCCCAGAGGT CAGGCGAGCA GGGAGAGACC 
ATGAAACCAT TTGACCTGAA CTACGGACAC GCCATCATTG AAAATTGCGG ATCCAGCCCT 
GGGGAGGAGA GTGAGGTGGG CTCTCTGGTG GGCGAAGGCT TCATTGAGGT CCTGACCAAG 
AAGCAGCGCC GCCTGCTGGA GGAGGAGAGG AGAAAGAAGG AGCAAGCCGT GCAGGTTCCT 
GTCAAAGGCC GAAGTCTCTC CTCTCGAATT CCTCCTCGGT TTGCAAAGAA GCAGAACAAC 
CTGTGCCTGG AGCAGGGTGA TGGGCCTGTG CCCGGCAGCA GCCTGGGCAC CGAGATCTGG 
GAGAGCGGCA GCCAGGCCCT CCCCCTTCAG GCCTCGACCA GTGACTCCTG GACCAAAGCT 
GCCACTGCCT TCACCAGCGC TGAGCCTGGC TCTGCTGAGC AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG TGATGCCGGC TGCCGCTGCC CAGCAAATCC CAATTTCCCT TCACACGTCT 
CTACAAGCTC AAGCCCAGCT GGGACTGAGG GGCGGGCTGC CCGTCTCCCA GTCTCAGGAG 
ATCTTCAGCT CCTTACAGCC CTTCAGGTCT CAGGTGTACA TGCACCCCAG CCTGTCACCG 
CCCAGCACCA TGATCCTCTC CGGAGGCACA GCCTTGAAGG CCCCCTACTC GGCGTTCCCA 
GGCATGCAGC CCCTGGAGAT GGTGAAGCCC CAGTCCGGCT CGCCCTACCA GCCCATGAGT 
GGGAGCCAGG CCCTGGTCTA TGAGGGCCCG CTCAGCCAGG CGGCTGGGCT TGGCGCCTCC 
CAGATGTTGG ACTCCCAGCT CCCGCAGCTG ACGATGCCCC TGCCTGGCTC CCAGCTGCCT 
CTGCCCCGGT ACGGCTCCGG GCAGCAGCCC CTGCTCCTGC CACAGTCCAT CCAGCTGCCC 
CAGGCGCAGA GCCTGTCAGT CGGGGCCCCC CGCAGGATCC TTGCCCCCGG GTCCCAGCCT 
TCGGTCCTCA ACACCAGCAG AGAGTCCTCT CAGATGGAGA TGAAGGGCTT CCACTTTGCC 
GACAGTAAAC AGAATGTTCC TTCAGGAGGC TCCGTGCCGT CACCACAGGC CTACAGGCCT 
AGCTCTGCTA GCCCCAGTGG GAAGCCCTCT GGATCAGCAG TTAACATGGG CTCTGTGCAG 
GGACACTACG TTCAACAGGC AAAGCAGCGA GTGGATGAAA AGCCCGGCCT GGGAGCCATG 
AAGCTGCAGG AGCCACCCTC AGCCGCTTCC CCGCTGAAGC GAACTGGAGC AATCAAGCCC 
CGGGCGGTCA AGGTGGAGGA GAGCAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855365.1
CDS	311..7003
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKCWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999597.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999597.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GTTGGGACCC CAGGAGGCAG CGGCAGTTAT CCTTGAGCTC TGCAGACAGT 
GCTGATGCCA AGCGCACCCA AGAGGAAGGA AAAGACTGGA GTGAAACAGG AGGCGTGACC 
CGTGTCGTCC GGAAGGTGCC GGAACCTCAG CCGCCTTCCA GGAAGCTGCA CAGCTGGGCG 
TCAGGCCCTG ACTACCAGCA GAAATCCTCC ATGGGCACTG TATTTCGGCA GCAGTCTGCC 
GAGGACAAAG AGGACAAGCC CCCACCGCGG CAGAAGTTTG TCCAGTCTGA GATGTCCGAG 
GCTGTGGAGC GAGCCCGGAA GCGCCGGGAG GAGGAGGAGC GCCGTGCGCG CGAGGAAAGG 
CTGGCTGCCT GTGCTGCCAA ACTCAAGCAG CTTGACCAGA AGTGCAAGCA GGCTCAGCGG 
GCCAGCGAGG CCCAGAAGCA GGCGGAGAAG GAAGCTCCCC GCTCTCCGGG GGCTGAGAAG 
GTGACTCCCC AGGAGAACGG CCCTGCCGTC CACAAAGGCT CCCCTGAGTT CTCTGCCCAG 
GAGACCGCCA GCACGTTTTC AGAAGAAGCC CCCACAGCTC CTCCAGCAGT GGCTCAGAGC 
GGCGGCAGTG AGGAGGGGCC CCGGGAGGCT GGCTCCCCTG CACAGGAATT CAACAAGTAC 
CAAAAGTCAC TCCCTCCCAG ATTCCAGCGC CAGCAGCAGC AGCAGCAGCA GGAGCAGCTG 
TACAAGATGC AGCACTGGCA GCCGGTGTAC CCTCCGCCGT CCCACCCGCA GCGCACCTTC 
TACCCGCACC ACCCCCAGAT GCTGGGCTTC GACCCCAGGT GGATGATGAT GCCCTCCTAC 
ATGGACCCAC GGATCCCGCC CACTCGGACC CCAGTGGATT TCTACCCCTC GGCCCTGCAT 
CCCTCAGGAT TGATGAAACC CATAATGCCC CCGGATTCCC TCAGTGGGAC TGGCTGTCGC 
TCTGAGGACC AGAGCTGTGT GCCCCCGCTG CCAGAAAGGA AGGTGACCGC CATTGACCCT 
GCCCCCGTGT GGAGCCCTGA GGGTTACATG GCGTTGCAGA GCAAGGGCTA CTCGCTGCCC 
CATCCGAAAT CCAGCGACAA TATGGCCATG GACATGCATG TCAGGAGTGA AAGCTCTTAC 
TCTGCCTCCC CCGGAAGGTC AGGGGGCGTG AGTGCCCAGC GCGAGCTCCT TGAGGAGAGA 
GGGCAGGAGT ACTTGAGCGC TTCTGACAAG AAGGCCCCAG CAGACTGTGA CTCCCGTGTC 
TCCTCTCAGA GAGTAGGCCA GGAGCTTTTG TTTCCACCCC AAGAAAATGG GCAGGAAGCA 
GGCACTCCTG GGAGTCACAC CCCAAACCTC AGGTGCTCCC CACTGGAGCC CGACTTTGCC 
CCCACGGAGA AAAAGCTCGA GTATGGTGAC TGGGATGTGA ACCACCAGTC AAAGGTTGCC 
AACCCAGACG CTGCGGTTGA GAAGGAGGTA CCGCGGGAGG AGCCACCCTT CAGCGTCTCC 
TCCTGGGAGA AGGAAGGGAG CCCGAGCAAG CCGCCGGCTC TGGAGCCCGA GTGGACGCCA 
GAAGCCCGGG GCACTGGCAG CCAGCACCCG GAGCAGACCA GCAGGACCCG TAGGTCCGGC 
CCCATCAAGA AACCTGTCCT GAAGGCCCTC AAGGTGGAGG ACAAGGAGAA GGAGCTCGAG 
AAAGTAAAGC CGGAGCTGGC AGAGGGCAGC ACCCGCCTGG CCCGGGAGCC AGGGCCTGTC 
CATGAAGTGG CTGAGGACGA GGACCAAGAG AACGACCCCG CACTGGCCAA TGCGGCCCCG 
GCCACCTCGG AGGACCAGGG CTTAGCCCGT GCCAGCTTGG GCCGTGAGGC CAGCCGTCCT 
GAGGAGGACA ACAAGCCGGA TGGGGCCCCG GAGGCCAGAC CCTCCAGGGA GGCCAGCCAC 
ACACCCCCGA CCAAGAGGAA TAACTGGATC TTTATTGATG AGGAGCAAGC CTTTGGGGGC 
CGGGGGCCAG CCCGGGGCCG AGGCCGCGGG TTCCGAGAGT TCACCTTCCG CGGGCGGCCC 
ACCGGTAGTG GCCTGTGTGG CGGGGGCGTC CTTGGGGCAC GCGGCCTCTA CAGCGGCAGC 
CAGAGGAGTA GCCGGGGCCG GGGGCCGCGG GAGTTTGGGC CGCCGGATGA CTTCCCCCGG 
GCCAAGCCCC GGCGGAGGAT TGCCAGTGAG ACCCACAGCG AGGGCTCTGA GTATGAGGAG 
CTCCCCAAGC GGCGGCGGCA GCGAGGCACT GAGCCTGGGG ATGAGGGTGC GCTCCCGGAG 
CGGGAGGAGG CCACGGGAAG GAAGGGCGAC TTCAGGGAGT CCTGGCGGTC CGGCAGGATG 
CACCCTGAGG ACCATGGTGG GCCTGAGGCC AAGAACCGAG GTCCCCGGGC CTTCGGGCGT 
GCCCTGCCCC CGCGTCTGAG CAACTGTGCA TATGGCCGGA GAACCTTCGC GGCCAAGGAG 
GTGGCCCCTT GGCCCAGCCG GGGTCCTGGC GCCTCCTGGC AGGAGTCTGG CGCCTCTGAC 
GCATGTGGAG CCCGGCGGCC CTTAGACAGA GACTACATCC CAGACGCCTA CAGACGCCCC 
GACGCCTTGG GCTCCCGGGG CTTCGAGGAT GGCCGCCTGG AGGACCGGAG GGCCTTCTTC 
CAAGATGACC ACGCCGCAGA GTCCGAAAAC GCAGAGAACC GGCCCTTCCG GCGAAGGCGG 
CCTCCGCGCC AGGACAAGCC GCCCCGCTTC CGGCGCCTCC GGCAGGAACG GGAGGCCCTG 
GGCCTGTGGG GGCCGGAGGA CGAGCCACCT CTGGCAGCCG GCCAGTGGCC AGGCCGTCCT 
AGACCCTGCC CCGGGGACAG GAGCAGCGCC TCAGGCCGCA GGTCCCCTGA GCTCGCCTAT 
CAGAACTCCT CCGACCACGC CAACGAGGAG TGGGAGACGG CCTCCGAGAG CAGTGACTTC 
AGCGAGAGGC GGGAGCGGCG GGCCGGCTCC GAGCTGGACC CCCAGGCTGA TGGCAGCCCC 
TCTGGGGTGA GCGCAGGCGA GAAGAAGGAG CTGGCCAAGC GGAGCTTCTC GGGCCAGAGA 
CCCCTGGTGG ACAGGCAGAG CCGGAAGCTG GAGCCGGGAG GGTTTGGGGA GAAGTCTGTT 
AGGCCAGGTG GCGGTGACAC TTCTCCCCGC TATGAGAGTC ATCAGAACGG GACGCCTCTG 
AAAGCCAAAA GGTCCCCAGA CGAGGCCTTG CCTGGAGGTC TTGGCTGCGG CAGTGGGAGC 
GGCCATTACA CGCTGGAGCG GGCAGCCCAT GCTGCCTCTG ACGTCACCGA AGCCTCCTGT 
GAGAAGGTAG AGAAGGAGGC CAAGCTGGCC GCCCAGAGGT CAGGCGAGCA GGGAGAGACC 
ATGAAACCAT TTGACCTGAA CTACGGACAC GCCATCATTG AAAATTGCGG ATCCAGCCCT 
GGGGAGGAGA GTGAGGTGGG CTCTCTGGTG GGCGAAGGCT TCATTGAGGT CCTGACCAAG 
AAGCAGCGCC GCCTGCTGGA GGAGGAGAGG AGAAAGAAGG AGCAAGCCGT GCAGGTTCCT 
GTCAAAGGCC GAAGTCTCTC CTCTCGAATT CCTCCTCGGT TTGCAAAGAA GCAGAACAAC 
CTGTGCCTGG AGCAGGGTGA TGGGCCTGTG CCCGGCAGCA GCCTGGGCAC CGAGATCTGG 
GAGAGCGGCA GCCAGGCCCT CCCCCTTCAG GCCTCGACCA GTGACTCCTG GACCAAAGCT 
GCCACTGCCT TCACCAGCGC TGAGCCTGGC TCTGCTGAGC AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG TGATGCCGGC TGCCGCTGCC CAGCAAATCC CAATTTCCCT TCACACGTCT 
CTACAAGCTC AAGCCCAGCT GGGACTGAGG GGCGGGCTGC CCGTCTCCCA GTCTCAGGAG 
ATCTTCAGCT CCTTACAGCC CTTCAGGTCT CAGGTGTACA TGCACCCCAG CCTGTCACCG 
CCCAGCACCA TGATCCTCTC CGGAGGCACA GCCTTGAAGG CCCCCTACTC GGCGTTCCCA 
GGCATGCAGC CCCTGGAGAT GGTGAAGCCC CAGTCCGGCT CGCCCTACCA GCCCATGAGT 
GGGAGCCAGG CCCTGGTCTA TGAGGGCCCG CTCAGCCAGG CGGCTGGGCT TGGCGCCTCC 
CAGATGTTGG ACTCCCAGCT CCCGCAGCTG ACGATGCCCC TGCCTGGCTC CCAGCTGCCT 
CTGCCCCGGT ACGGCTCCGG GCAGCAGCCC CTGCTCCTGC CACAGTCCAT CCAGCTGCCC 
CAGGCGCAGA GCCTGTCAGT CGGGGCCCCC CGCAGGATCC TTGCCCCCGG GTCCCAGCCT 
TCGGTCCTCA ACACCAGCAG AGAGTCCTCT CAGATGGAGA TGAAGGGCTT CCACTTTGCC 
GACAGTAAAC AGAATGTTCC TTCAGGAGGC TCCGTGCCGT CACCACAGGC CTACAGGCCT 
AGCTCTGCTA GCCCCAGTGG GAAGCCCTCT GGATCAGCAG TTAACATGGG CTCTGTGCAG 
GGACACTACG TTCAACAGGC AAAGCAGCGA GTGGATGAAA AGCCCGGCCT GGGAGCCATG 
AAGCTGCAGG AGCCACCCTC AGCCGCTTCC CCGCTGAAGC GAACTGGAGC AATCAAGCCC 
CGGGCGGTCA AGGTGGAGGA GAGCAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213444.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213501.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.

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CloneID	OBa24518
Clone ID Related Accession (Same CDS sequence)	XM_015465513.1 , XM_024999597.1 , XM_005213444.3
Accession Version	XM_005213444.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GTTGGGACCC CAGGAGGCAG CGGCAGTTAT CCTTGAGCTC TGCAGACAGT 
GCTGATGCCA AGCGCACCCA AGAGGAAGGA AAAGACTGGA GTGAAACAGG AGGCGTGACC 
CGTGTCGTCC GGAAGGTGCC GGAACCTCAG CCGCCTTCCA GGAAGCTGCA CAGCTGGGCG 
TCAGGCCCTG ACTACCAGCA GAAATCCTCC ATGGGCACTG TATTTCGGCA GCAGTCTGCC 
GAGGACAAAG AGGACAAGCC CCCACCGCGG CAGAAGTTTG TCCAGTCTGA GATGTCCGAG 
GCTGTGGAGC GAGCCCGGAA GCGCCGGGAG GAGGAGGAGC GCCGTGCGCG CGAGGAAAGG 
CTGGCTGCCT GTGCTGCCAA ACTCAAGCAG CTTGACCAGA AGTGCAAGCA GGCTCAGCGG 
GCCAGCGAGG CCCAGAAGCA GGCGGAGAAG GAAGCTCCCC GCTCTCCGGG GGCTGAGAAG 
GTGACTCCCC AGGAGAACGG CCCTGCCGTC CACAAAGGCT CCCCTGAGTT CTCTGCCCAG 
GAGACCGCCA GCACGTTTTC AGAAGAAGCC CCCACAGCTC CTCCAGCAGT GGCTCAGAGC 
GGCGGCAGTG AGGAGGGGCC CCGGGAGGCT GGCTCCCCTG CACAGGAATT CAACAAGTAC 
CAAAAGTCAC TCCCTCCCAG ATTCCAGCGC CAGCAGCAGC AGCAGCAGCA GGAGCAGCTG 
TACAAGATGC AGCACTGGCA GCCGGTGTAC CCTCCGCCGT CCCACCCGCA GCGCACCTTC 
TACCCGCACC ACCCCCAGAT GCTGGGCTTC GACCCCAGGT GGATGATGAT GCCCTCCTAC 
ATGGACCCAC GGATCCCGCC CACTCGGACC CCAGTGGATT TCTACCCCTC GGCCCTGCAT 
CCCTCAGGAT TGATGAAACC CATAATGCCC CCGGATTCCC TCAGTGGGAC TGGCTGTCGC 
TCTGAGGACC AGAGCTGTGT GCCCCCGCTG CCAGAAAGGA AGGTGACCGC CATTGACCCT 
GCCCCCGTGT GGAGCCCTGA GGGTTACATG GCGTTGCAGA GCAAGGGCTA CTCGCTGCCC 
CATCCGAAAT CCAGCGACAA TATGGCCATG GACATGCATG TCAGGAGTGA AAGCTCTTAC 
TCTGCCTCCC CCGGAAGGTC AGGGGGCGTG AGTGCCCAGC GCGAGCTCCT TGAGGAGAGA 
GGGCAGGAGT ACTTGAGCGC TTCTGACAAG AAGGCCCCAG CAGACTGTGA CTCCCGTGTC 
TCCTCTCAGA GAGTAGGCCA GGAGCTTTTG TTTCCACCCC AAGAAAATGG GCAGGAAGCA 
GGCACTCCTG GGAGTCACAC CCCAAACCTC AGGTGCTCCC CACTGGAGCC CGACTTTGCC 
CCCACGGAGA AAAAGCTCGA GTATGGTGAC TGGGATGTGA ACCACCAGTC AAAGGTTGCC 
AACCCAGACG CTGCGGTTGA GAAGGAGGTA CCGCGGGAGG AGCCACCCTT CAGCGTCTCC 
TCCTGGGAGA AGGAAGGGAG CCCGAGCAAG CCGCCGGCTC TGGAGCCCGA GTGGACGCCA 
GAAGCCCGGG GCACTGGCAG CCAGCACCCG GAGCAGACCA GCAGGACCCG TAGGTCCGGC 
CCCATCAAGA AACCTGTCCT GAAGGCCCTC AAGGTGGAGG ACAAGGAGAA GGAGCTCGAG 
AAAGTAAAGC CGGAGCTGGC AGAGGGCAGC ACCCGCCTGG CCCGGGAGCC AGGGCCTGTC 
CATGAAGTGG CTGAGGACGA GGACCAAGAG AACGACCCCG CACTGGCCAA TGCGGCCCCG 
GCCACCTCGG AGGACCAGGG CTTAGCCCGT GCCAGCTTGG GCCGTGAGGC CAGCCGTCCT 
GAGGAGGACA ACAAGCCGGA TGGGGCCCCG GAGGCCAGAC CCTCCAGGGA GGCCAGCCAC 
ACACCCCCGA CCAAGAGGAA TAACTGGATC TTTATTGATG AGGAGCAAGC CTTTGGGGGC 
CGGGGGCCAG CCCGGGGCCG AGGCCGCGGG TTCCGAGAGT TCACCTTCCG CGGGCGGCCC 
ACCGGTAGTG GCCTGTGTGG CGGGGGCGTC CTTGGGGCAC GCGGCCTCTA CAGCGGCAGC 
CAGAGGAGTA GCCGGGGCCG GGGGCCGCGG GAGTTTGGGC CGCCGGATGA CTTCCCCCGG 
GCCAAGCCCC GGCGGAGGAT TGCCAGTGAG ACCCACAGCG AGGGCTCTGA GTATGAGGAG 
CTCCCCAAGC GGCGGCGGCA GCGAGGCACT GAGCCTGGGG ATGAGGGTGC GCTCCCGGAG 
CGGGAGGAGG CCACGGGAAG GAAGGGCGAC TTCAGGGAGT CCTGGCGGTC CGGCAGGATG 
CACCCTGAGG ACCATGGTGG GCCTGAGGCC AAGAACCGAG GTCCCCGGGC CTTCGGGCGT 
GCCCTGCCCC CGCGTCTGAG CAACTGTGCA TATGGCCGGA GAACCTTCGC GGCCAAGGAG 
GTGGCCCCTT GGCCCAGCCG GGGTCCTGGC GCCTCCTGGC AGGAGTCTGG CGCCTCTGAC 
GCATGTGGAG CCCGGCGGCC CTTAGACAGA GACTACATCC CAGACGCCTA CAGACGCCCC 
GACGCCTTGG GCTCCCGGGG CTTCGAGGAT GGCCGCCTGG AGGACCGGAG GGCCTTCTTC 
CAAGATGACC ACGCCGCAGA GTCCGAAAAC GCAGAGAACC GGCCCTTCCG GCGAAGGCGG 
CCTCCGCGCC AGGACAAGCC GCCCCGCTTC CGGCGCCTCC GGCAGGAACG GGAGGCCCTG 
GGCCTGTGGG GGCCGGAGGA CGAGCCACCT CTGGCAGCCG GCCAGTGGCC AGGCCGTCCT 
AGACCCTGCC CCGGGGACAG GAGCAGCGCC TCAGGCCGCA GGTCCCCTGA GCTCGCCTAT 
CAGAACTCCT CCGACCACGC CAACGAGGAG TGGGAGACGG CCTCCGAGAG CAGTGACTTC 
AGCGAGAGGC GGGAGCGGCG GGCCGGCTCC GAGCTGGACC CCCAGGCTGA TGGCAGCCCC 
TCTGGGGTGA GCGCAGGCGA GAAGAAGGAG CTGGCCAAGC GGAGCTTCTC GGGCCAGAGA 
CCCCTGGTGG ACAGGCAGAG CCGGAAGCTG GAGCCGGGAG GGTTTGGGGA GAAGTCTGTT 
AGGCCAGGTG GCGGTGACAC TTCTCCCCGC TATGAGAGTC ATCAGAACGG GACGCCTCTG 
AAAGCCAAAA GGTCCCCAGA CGAGGCCTTG CCTGGAGGTC TTGGCTGCGG CAGTGGGAGC 
GGCCATTACA CGCTGGAGCG GGCAGCCCAT GCTGCCTCTG ACGTCACCGA AGCCTCCTGT 
GAGAAGGTAG AGAAGGAGGC CAAGCTGGCC GCCCAGAGGT CAGGCGAGCA GGGAGAGACC 
ATGAAACCAT TTGACCTGAA CTACGGACAC GCCATCATTG AAAATTGCGG ATCCAGCCCT 
GGGGAGGAGA GTGAGGTGGG CTCTCTGGTG GGCGAAGGCT TCATTGAGGT CCTGACCAAG 
AAGCAGCGCC GCCTGCTGGA GGAGGAGAGG AGAAAGAAGG AGCAAGCCGT GCAGGTTCCT 
GTCAAAGGCC GAAGTCTCTC CTCTCGAATT CCTCCTCGGT TTGCAAAGAA GCAGAACAAC 
CTGTGCCTGG AGCAGGGTGA TGGGCCTGTG CCCGGCAGCA GCCTGGGCAC CGAGATCTGG 
GAGAGCGGCA GCCAGGCCCT CCCCCTTCAG GCCTCGACCA GTGACTCCTG GACCAAAGCT 
GCCACTGCCT TCACCAGCGC TGAGCCTGGC TCTGCTGAGC AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG TGATGCCGGC TGCCGCTGCC CAGCAAATCC CAATTTCCCT TCACACGTCT 
CTACAAGCTC AAGCCCAGCT GGGACTGAGG GGCGGGCTGC CCGTCTCCCA GTCTCAGGAG 
ATCTTCAGCT CCTTACAGCC CTTCAGGTCT CAGGTGTACA TGCACCCCAG CCTGTCACCG 
CCCAGCACCA TGATCCTCTC CGGAGGCACA GCCTTGAAGG CCCCCTACTC GGCGTTCCCA 
GGCATGCAGC CCCTGGAGAT GGTGAAGCCC CAGTCCGGCT CGCCCTACCA GCCCATGAGT 
GGGAGCCAGG CCCTGGTCTA TGAGGGCCCG CTCAGCCAGG CGGCTGGGCT TGGCGCCTCC 
CAGATGTTGG ACTCCCAGCT CCCGCAGCTG ACGATGCCCC TGCCTGGCTC CCAGCTGCCT 
CTGCCCCGGT ACGGCTCCGG GCAGCAGCCC CTGCTCCTGC CACAGTCCAT CCAGCTGCCC 
CAGGCGCAGA GCCTGTCAGT CGGGGCCCCC CGCAGGATCC TTGCCCCCGG GTCCCAGCCT 
TCGGTCCTCA ACACCAGCAG AGAGTCCTCT CAGATGGAGA TGAAGGGCTT CCACTTTGCC 
GACAGTAAAC AGAATGTTCC TTCAGGAGGC TCCGTGCCGT CACCACAGGC CTACAGGCCT 
AGCTCTGCTA GCCCCAGTGG GAAGCCCTCT GGATCAGCAG TTAACATGGG CTCTGTGCAG 
GGACACTACG TTCAACAGGC AAAGCAGCGA GTGGATGAAA AGCCCGGCCT GGGAGCCATG 
AAGCTGCAGG AGCCACCCTC AGCCGCTTCC CCGCTGAAGC GAACTGGAGC AATCAAGCCC 
CGGGCGGTCA AGGTGGAGGA GAGCAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213501.1
CDS	1412..8104
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKCWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_005213444.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213444.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GTTGGGACCC CAGGAGGCAG CGGCAGTTAT CCTTGAGCTC TGCAGACAGT 
GCTGATGCCA AGCGCACCCA AGAGGAAGGA AAAGACTGGA GTGAAACAGG AGGCGTGACC 
CGTGTCGTCC GGAAGGTGCC GGAACCTCAG CCGCCTTCCA GGAAGCTGCA CAGCTGGGCG 
TCAGGCCCTG ACTACCAGCA GAAATCCTCC ATGGGCACTG TATTTCGGCA GCAGTCTGCC 
GAGGACAAAG AGGACAAGCC CCCACCGCGG CAGAAGTTTG TCCAGTCTGA GATGTCCGAG 
GCTGTGGAGC GAGCCCGGAA GCGCCGGGAG GAGGAGGAGC GCCGTGCGCG CGAGGAAAGG 
CTGGCTGCCT GTGCTGCCAA ACTCAAGCAG CTTGACCAGA AGTGCAAGCA GGCTCAGCGG 
GCCAGCGAGG CCCAGAAGCA GGCGGAGAAG GAAGCTCCCC GCTCTCCGGG GGCTGAGAAG 
GTGACTCCCC AGGAGAACGG CCCTGCCGTC CACAAAGGCT CCCCTGAGTT CTCTGCCCAG 
GAGACCGCCA GCACGTTTTC AGAAGAAGCC CCCACAGCTC CTCCAGCAGT GGCTCAGAGC 
GGCGGCAGTG AGGAGGGGCC CCGGGAGGCT GGCTCCCCTG CACAGGAATT CAACAAGTAC 
CAAAAGTCAC TCCCTCCCAG ATTCCAGCGC CAGCAGCAGC AGCAGCAGCA GGAGCAGCTG 
TACAAGATGC AGCACTGGCA GCCGGTGTAC CCTCCGCCGT CCCACCCGCA GCGCACCTTC 
TACCCGCACC ACCCCCAGAT GCTGGGCTTC GACCCCAGGT GGATGATGAT GCCCTCCTAC 
ATGGACCCAC GGATCCCGCC CACTCGGACC CCAGTGGATT TCTACCCCTC GGCCCTGCAT 
CCCTCAGGAT TGATGAAACC CATAATGCCC CCGGATTCCC TCAGTGGGAC TGGCTGTCGC 
TCTGAGGACC AGAGCTGTGT GCCCCCGCTG CCAGAAAGGA AGGTGACCGC CATTGACCCT 
GCCCCCGTGT GGAGCCCTGA GGGTTACATG GCGTTGCAGA GCAAGGGCTA CTCGCTGCCC 
CATCCGAAAT CCAGCGACAA TATGGCCATG GACATGCATG TCAGGAGTGA AAGCTCTTAC 
TCTGCCTCCC CCGGAAGGTC AGGGGGCGTG AGTGCCCAGC GCGAGCTCCT TGAGGAGAGA 
GGGCAGGAGT ACTTGAGCGC TTCTGACAAG AAGGCCCCAG CAGACTGTGA CTCCCGTGTC 
TCCTCTCAGA GAGTAGGCCA GGAGCTTTTG TTTCCACCCC AAGAAAATGG GCAGGAAGCA 
GGCACTCCTG GGAGTCACAC CCCAAACCTC AGGTGCTCCC CACTGGAGCC CGACTTTGCC 
CCCACGGAGA AAAAGCTCGA GTATGGTGAC TGGGATGTGA ACCACCAGTC AAAGGTTGCC 
AACCCAGACG CTGCGGTTGA GAAGGAGGTA CCGCGGGAGG AGCCACCCTT CAGCGTCTCC 
TCCTGGGAGA AGGAAGGGAG CCCGAGCAAG CCGCCGGCTC TGGAGCCCGA GTGGACGCCA 
GAAGCCCGGG GCACTGGCAG CCAGCACCCG GAGCAGACCA GCAGGACCCG TAGGTCCGGC 
CCCATCAAGA AACCTGTCCT GAAGGCCCTC AAGGTGGAGG ACAAGGAGAA GGAGCTCGAG 
AAAGTAAAGC CGGAGCTGGC AGAGGGCAGC ACCCGCCTGG CCCGGGAGCC AGGGCCTGTC 
CATGAAGTGG CTGAGGACGA GGACCAAGAG AACGACCCCG CACTGGCCAA TGCGGCCCCG 
GCCACCTCGG AGGACCAGGG CTTAGCCCGT GCCAGCTTGG GCCGTGAGGC CAGCCGTCCT 
GAGGAGGACA ACAAGCCGGA TGGGGCCCCG GAGGCCAGAC CCTCCAGGGA GGCCAGCCAC 
ACACCCCCGA CCAAGAGGAA TAACTGGATC TTTATTGATG AGGAGCAAGC CTTTGGGGGC 
CGGGGGCCAG CCCGGGGCCG AGGCCGCGGG TTCCGAGAGT TCACCTTCCG CGGGCGGCCC 
ACCGGTAGTG GCCTGTGTGG CGGGGGCGTC CTTGGGGCAC GCGGCCTCTA CAGCGGCAGC 
CAGAGGAGTA GCCGGGGCCG GGGGCCGCGG GAGTTTGGGC CGCCGGATGA CTTCCCCCGG 
GCCAAGCCCC GGCGGAGGAT TGCCAGTGAG ACCCACAGCG AGGGCTCTGA GTATGAGGAG 
CTCCCCAAGC GGCGGCGGCA GCGAGGCACT GAGCCTGGGG ATGAGGGTGC GCTCCCGGAG 
CGGGAGGAGG CCACGGGAAG GAAGGGCGAC TTCAGGGAGT CCTGGCGGTC CGGCAGGATG 
CACCCTGAGG ACCATGGTGG GCCTGAGGCC AAGAACCGAG GTCCCCGGGC CTTCGGGCGT 
GCCCTGCCCC CGCGTCTGAG CAACTGTGCA TATGGCCGGA GAACCTTCGC GGCCAAGGAG 
GTGGCCCCTT GGCCCAGCCG GGGTCCTGGC GCCTCCTGGC AGGAGTCTGG CGCCTCTGAC 
GCATGTGGAG CCCGGCGGCC CTTAGACAGA GACTACATCC CAGACGCCTA CAGACGCCCC 
GACGCCTTGG GCTCCCGGGG CTTCGAGGAT GGCCGCCTGG AGGACCGGAG GGCCTTCTTC 
CAAGATGACC ACGCCGCAGA GTCCGAAAAC GCAGAGAACC GGCCCTTCCG GCGAAGGCGG 
CCTCCGCGCC AGGACAAGCC GCCCCGCTTC CGGCGCCTCC GGCAGGAACG GGAGGCCCTG 
GGCCTGTGGG GGCCGGAGGA CGAGCCACCT CTGGCAGCCG GCCAGTGGCC AGGCCGTCCT 
AGACCCTGCC CCGGGGACAG GAGCAGCGCC TCAGGCCGCA GGTCCCCTGA GCTCGCCTAT 
CAGAACTCCT CCGACCACGC CAACGAGGAG TGGGAGACGG CCTCCGAGAG CAGTGACTTC 
AGCGAGAGGC GGGAGCGGCG GGCCGGCTCC GAGCTGGACC CCCAGGCTGA TGGCAGCCCC 
TCTGGGGTGA GCGCAGGCGA GAAGAAGGAG CTGGCCAAGC GGAGCTTCTC GGGCCAGAGA 
CCCCTGGTGG ACAGGCAGAG CCGGAAGCTG GAGCCGGGAG GGTTTGGGGA GAAGTCTGTT 
AGGCCAGGTG GCGGTGACAC TTCTCCCCGC TATGAGAGTC ATCAGAACGG GACGCCTCTG 
AAAGCCAAAA GGTCCCCAGA CGAGGCCTTG CCTGGAGGTC TTGGCTGCGG CAGTGGGAGC 
GGCCATTACA CGCTGGAGCG GGCAGCCCAT GCTGCCTCTG ACGTCACCGA AGCCTCCTGT 
GAGAAGGTAG AGAAGGAGGC CAAGCTGGCC GCCCAGAGGT CAGGCGAGCA GGGAGAGACC 
ATGAAACCAT TTGACCTGAA CTACGGACAC GCCATCATTG AAAATTGCGG ATCCAGCCCT 
GGGGAGGAGA GTGAGGTGGG CTCTCTGGTG GGCGAAGGCT TCATTGAGGT CCTGACCAAG 
AAGCAGCGCC GCCTGCTGGA GGAGGAGAGG AGAAAGAAGG AGCAAGCCGT GCAGGTTCCT 
GTCAAAGGCC GAAGTCTCTC CTCTCGAATT CCTCCTCGGT TTGCAAAGAA GCAGAACAAC 
CTGTGCCTGG AGCAGGGTGA TGGGCCTGTG CCCGGCAGCA GCCTGGGCAC CGAGATCTGG 
GAGAGCGGCA GCCAGGCCCT CCCCCTTCAG GCCTCGACCA GTGACTCCTG GACCAAAGCT 
GCCACTGCCT TCACCAGCGC TGAGCCTGGC TCTGCTGAGC AGGGCTTTAA GAGCAGCCAG 
GGAGACAGCG GTGTTGACCT GAGCGCCGAG TCCCGAGAGT CCTCAGCGAC CTCCTCGCAG 
CGCAGCTCTC CATATGGCAC CCTGAAGCCC GAGGAGGCAA GCGGGCCCGG CCTGGAGCCC 
AAGGCCGATG GCCACAAGGA GCAGACTCAA AAGCAGTCAG AGCCAAAGGA TTCAGAACAA 
GGCTCAGGAC AGAGCAAGGA GCACAGACCA GGACCCATTG GCAATGAGCG CTCTCTGAAA 
AACAGAAAGG GCTCCGAGGG GGCTGAACGG CTGCAGGGGG CTGTGGTTCC CCCTGTTAAC 
GGGGTGGAGA TTCACGTGGA CTCTGTGCTG CCGGTGCCAC CCATTGAATT TGGAGTCAAT 
CCGAAGGACT CTGATTTCAG CTTGCAGCCT GGCTCTGCCT CTGGTCCTGC GGGGAATCCA 
GTTGTCAAGC TTCAGGACGC ATTGGCCAGT AACGCCGGGC TGGCCCCCAG CATCCCCATC 
CTGCGGCGGG ACCACCACAT CCAGAGGGCC ATCGGCCTCT CCCACATGTC CTTCCCTACC 
GCCGACCTCA CTCTGAAGAT GGAGTCTGCG CGCAAGGCTT GGGAAAACTC GCCCAGCTTG 
CCGGAGCAGA GCTCGCCAGG AGGCGCCGGC TCAGGCCTGC AGCCCCCATC CTCCGGGGGA 
ACCTCCAGCG GGGTCAGCTA TAGCTCCTTT GGCGGCGTCT CCATGCCCCC CGTGCCAGTG 
GCCTCGGTCG CACCTTCTGC ATCCCTTCCA GGCAGCCACC TGCCACCTCT CTACCTGGAT 
GGCCATGTGT TTGCAAGTCA GCCCCGACTG GTTCCTCAGA CCATACCTCA GCAGCAGAGT 
TTCCAACAGG TGATGCCGGC TGCCGCTGCC CAGCAAATCC CAATTTCCCT TCACACGTCT 
CTACAAGCTC AAGCCCAGCT GGGACTGAGG GGCGGGCTGC CCGTCTCCCA GTCTCAGGAG 
ATCTTCAGCT CCTTACAGCC CTTCAGGTCT CAGGTGTACA TGCACCCCAG CCTGTCACCG 
CCCAGCACCA TGATCCTCTC CGGAGGCACA GCCTTGAAGG CCCCCTACTC GGCGTTCCCA 
GGCATGCAGC CCCTGGAGAT GGTGAAGCCC CAGTCCGGCT CGCCCTACCA GCCCATGAGT 
GGGAGCCAGG CCCTGGTCTA TGAGGGCCCG CTCAGCCAGG CGGCTGGGCT TGGCGCCTCC 
CAGATGTTGG ACTCCCAGCT CCCGCAGCTG ACGATGCCCC TGCCTGGCTC CCAGCTGCCT 
CTGCCCCGGT ACGGCTCCGG GCAGCAGCCC CTGCTCCTGC CACAGTCCAT CCAGCTGCCC 
CAGGCGCAGA GCCTGTCAGT CGGGGCCCCC CGCAGGATCC TTGCCCCCGG GTCCCAGCCT 
TCGGTCCTCA ACACCAGCAG AGAGTCCTCT CAGATGGAGA TGAAGGGCTT CCACTTTGCC 
GACAGTAAAC AGAATGTTCC TTCAGGAGGC TCCGTGCCGT CACCACAGGC CTACAGGCCT 
AGCTCTGCTA GCCCCAGTGG GAAGCCCTCT GGATCAGCAG TTAACATGGG CTCTGTGCAG 
GGACACTACG TTCAACAGGC AAAGCAGCGA GTGGATGAAA AGCCCGGCCT GGGAGCCATG 
AAGCTGCAGG AGCCACCCTC AGCCGCTTCC CCGCTGAAGC GAACTGGAGC AATCAAGCCC 
CGGGCGGTCA AGGTGGAGGA GAGCAAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213445.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213502.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.

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CloneID	OBa24519
Clone ID Related Accession (Same CDS sequence)	XM_005213445.3 , XM_015465514.1 , XM_024999598.1
Accession Version	XM_005213445.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213445.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCCTAGC 
TCTGCTAGCC CCAGTGGGAA GCCCTCTGGA TCAGCAGTTA ACATGGGCTC TGTGCAGGGA 
CACTACGTTC AACAGGCAAA GCAGCGAGTG GATGAAAAGC CCGGCCTGGG AGCCATGAAG 
CTGCAGGAGC CACCCTCAGC CGCTTCCCCG CTGAAGCGAA CTGGAGCAAT CAAGCCCCGG 
GCGGTCAAGG TGGAGGAGAG CAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213502.1
CDS	1411..8100
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_005213445.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213445.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCCTAGC 
TCTGCTAGCC CCAGTGGGAA GCCCTCTGGA TCAGCAGTTA ACATGGGCTC TGTGCAGGGA 
CACTACGTTC AACAGGCAAA GCAGCGAGTG GATGAAAAGC CCGGCCTGGG AGCCATGAAG 
CTGCAGGAGC CACCCTCAGC CGCTTCCCCG CTGAAGCGAA CTGGAGCAAT CAAGCCCCGG 
GCGGTCAAGG TGGAGGAGAG CAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465514.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321000.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.

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CloneID	OBa24519
Clone ID Related Accession (Same CDS sequence)	XM_005213445.3 , XM_015465514.1 , XM_024999598.1
Accession Version	XM_015465514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCCTAGC 
TCTGCTAGCC CCAGTGGGAA GCCCTCTGGA TCAGCAGTTA ACATGGGCTC TGTGCAGGGA 
CACTACGTTC AACAGGCAAA GCAGCGAGTG GATGAAAAGC CCGGCCTGGG AGCCATGAAG 
CTGCAGGAGC CACCCTCAGC CGCTTCCCCG CTGAAGCGAA CTGGAGCAAT CAAGCCCCGG 
GCGGTCAAGG TGGAGGAGAG CAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321000.1
CDS	1411..8100
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465514.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465514.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCCTAGC 
TCTGCTAGCC CCAGTGGGAA GCCCTCTGGA TCAGCAGTTA ACATGGGCTC TGTGCAGGGA 
CACTACGTTC AACAGGCAAA GCAGCGAGTG GATGAAAAGC CCGGCCTGGG AGCCATGAAG 
CTGCAGGAGC CACCCTCAGC CGCTTCCCCG CTGAAGCGAA CTGGAGCAAT CAAGCCCCGG 
GCGGTCAAGG TGGAGGAGAG CAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999598.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855366.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.

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CloneID	OBa24519
Clone ID Related Accession (Same CDS sequence)	XM_005213445.3 , XM_015465514.1 , XM_024999598.1
Accession Version	XM_024999598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCCTAGC 
TCTGCTAGCC CCAGTGGGAA GCCCTCTGGA TCAGCAGTTA ACATGGGCTC TGTGCAGGGA 
CACTACGTTC AACAGGCAAA GCAGCGAGTG GATGAAAAGC CCGGCCTGGG AGCCATGAAG 
CTGCAGGAGC CACCCTCAGC CGCTTCCCCG CTGAAGCGAA CTGGAGCAAT CAAGCCCCGG 
GCGGTCAAGG TGGAGGAGAG CAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855366.1
CDS	311..7000
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999598.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999598.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGCTGC CGCTGCCCAG CAAATCCCAA TTTCCCTTCA CACGTCTCTA 
CAAGCTCAAG CCCAGCTGGG ACTGAGGGGC GGGCTGCCCG TCTCCCAGTC TCAGGAGATC 
TTCAGCTCCT TACAGCCCTT CAGGTCTCAG GTGTACATGC ACCCCAGCCT GTCACCGCCC 
AGCACCATGA TCCTCTCCGG AGGCACAGCC TTGAAGGCCC CCTACTCGGC GTTCCCAGGC 
ATGCAGCCCC TGGAGATGGT GAAGCCCCAG TCCGGCTCGC CCTACCAGCC CATGAGTGGG 
AGCCAGGCCC TGGTCTATGA GGGCCCGCTC AGCCAGGCGG CTGGGCTTGG CGCCTCCCAG 
ATGTTGGACT CCCAGCTCCC GCAGCTGACG ATGCCCCTGC CTGGCTCCCA GCTGCCTCTG 
CCCCGGTACG GCTCCGGGCA GCAGCCCCTG CTCCTGCCAC AGTCCATCCA GCTGCCCCAG 
GCGCAGAGCC TGTCAGTCGG GGCCCCCCGC AGGATCCTTG CCCCCGGGTC CCAGCCTTCG 
GTCCTCAACA CCAGCAGAGA GTCCTCTCAG ATGGAGATGA AGGGCTTCCA CTTTGCCGAC 
AGTAAACAGA ATGTTCCTTC AGGAGGCTCC GTGCCGTCAC CACAGGCCTA CAGGCCTAGC 
TCTGCTAGCC CCAGTGGGAA GCCCTCTGGA TCAGCAGTTA ACATGGGCTC TGTGCAGGGA 
CACTACGTTC AACAGGCAAA GCAGCGAGTG GATGAAAAGC CCGGCCTGGG AGCCATGAAG 
CTGCAGGAGC CACCCTCAGC CGCTTCCCCG CTGAAGCGAA CTGGAGCAAT CAAGCCCCGG 
GCGGTCAAGG TGGAGGAGAG CAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465515.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321001.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.

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CloneID	OBa24520
Clone ID Related Accession (Same CDS sequence)	XM_015465515.1 , XM_024999599.1 , XM_002691647.5
Accession Version	XM_015465515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321001.1
CDS	1..6687
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_015465515.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465515.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999599.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855367.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.

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CloneID	OBa24520
Clone ID Related Accession (Same CDS sequence)	XM_015465515.1 , XM_024999599.1 , XM_002691647.5
Accession Version	XM_024999599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855367.1
CDS	311..6997
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_024999599.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999599.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_002691647.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691693.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.

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CloneID	OBa24520
Clone ID Related Accession (Same CDS sequence)	XM_015465515.1 , XM_024999599.1 , XM_002691647.5
Accession Version	XM_002691647.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691647.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691693.1
CDS	1..6687
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRPSSASPSGKPSGSAVNMGSVQGHYVQQAKQRVDEKPGLGAMKLQEPPSAASPLKRTGAIKPRAVKVEESKA

Target ORF information:

RefSeq Version	XM_002691647.5
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691647.5

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGAAATCC TCCATGGGCA CTGTATTTCG GCAGCAGTCT 
GCCGAGGACA AAGAGGACAA GCCCCCACCG CGGCAGAAGT TTGTCCAGTC TGAGATGTCC 
GAGGCTGTGG AGCGAGCCCG GAAGCGCCGG GAGGAGGAGG AGCGCCGTGC GCGCGAGGAA 
AGGCTGGCTG CCTGTGCTGC CAAACTCAAG CAGCTTGACC AGAAGTGCAA GCAGGCTCAG 
CGGGCCAGCG AGGCCCAGAA GCAGGCGGAG AAGGAAGCTC CCCGCTCTCC GGGGGCTGAG 
AAGGTGACTC CCCAGGAGAA CGGCCCTGCC GTCCACAAAG GCTCCCCTGA GTTCTCTGCC 
CAGGAGACCG CCAGCACGTT TTCAGAAGAA GCCCCCACAG CTCCTCCAGC AGTGGCTCAG 
AGCGGCGGCA GTGAGGAGGG GCCCCGGGAG GCTGGCTCCC CTGCACAGGA ATTCAACAAG 
TACCAAAAGT CACTCCCTCC CAGATTCCAG CGCCAGCAGC AGCAGCAGCA GCAGGAGCAG 
CTGTACAAGA TGCAGCACTG GCAGCCGGTG TACCCTCCGC CGTCCCACCC GCAGCGCACC 
TTCTACCCGC ACCACCCCCA GATGCTGGGC TTCGACCCCA GGTGGATGAT GATGCCCTCC 
TACATGGACC CACGGATCCC GCCCACTCGG ACCCCAGTGG ATTTCTACCC CTCGGCCCTG 
CATCCCTCAG GATTGATGAA ACCCATAATG CCCCCGGATT CCCTCAGTGG GACTGGCTGT 
CGCTCTGAGG ACCAGAGCTG TGTGCCCCCG CTGCCAGAAA GGAAGGTGAC CGCCATTGAC 
CCTGCCCCCG TGTGGAGCCC TGAGGGTTAC ATGGCGTTGC AGAGCAAGGG CTACTCGCTG 
CCCCATCCGA AATCCAGCGA CAATATGGCC ATGGACATGC ATGTCAGGAG TGAAAGCTCT 
TACTCTGCCT CCCCCGGAAG GTCAGGGGGC GTGAGTGCCC AGCGCGAGCT CCTTGAGGAG 
AGAGGGCAGG AGTACTTGAG CGCTTCTGAC AAGAAGGCCC CAGCAGACTG TGACTCCCGT 
GTCTCCTCTC AGAGAGTAGG CCAGGAGCTT TTGTTTCCAC CCCAAGAAAA TGGGCAGGAA 
GCAGGCACTC CTGGGAGTCA CACCCCAAAC CTCAGGTGCT CCCCACTGGA GCCCGACTTT 
GCCCCCACGG AGAAAAAGCT CGAGTATGGT GACTGGGATG TGAACCACCA GTCAAAGGTT 
GCCAACCCAG ACGCTGCGGT TGAGAAGGAG GTACCGCGGG AGGAGCCACC CTTCAGCGTC 
TCCTCCTGGG AGAAGGAAGG GAGCCCGAGC AAGCCGCCGG CTCTGGAGCC CGAGTGGACG 
CCAGAAGCCC GGGGCACTGG CAGCCAGCAC CCGGAGCAGA CCAGCAGGAC CCGTAGGTCC 
GGCCCCATCA AGAAACCTGT CCTGAAGGCC CTCAAGGTGG AGGACAAGGA GAAGGAGCTC 
GAGAAAGTAA AGCCGGAGCT GGCAGAGGGC AGCACCCGCC TGGCCCGGGA GCCAGGGCCT 
GTCCATGAAG TGGCTGAGGA CGAGGACCAA GAGAACGACC CCGCACTGGC CAATGCGGCC 
CCGGCCACCT CGGAGGACCA GGGCTTAGCC CGTGCCAGCT TGGGCCGTGA GGCCAGCCGT 
CCTGAGGAGG ACAACAAGCC GGATGGGGCC CCGGAGGCCA GACCCTCCAG GGAGGCCAGC 
CACACACCCC CGACCAAGAG GAATAACTGG ATCTTTATTG ATGAGGAGCA AGCCTTTGGG 
GGCCGGGGGC CAGCCCGGGG CCGAGGCCGC GGGTTCCGAG AGTTCACCTT CCGCGGGCGG 
CCCACCGGTA GTGGCCTGTG TGGCGGGGGC GTCCTTGGGG CACGCGGCCT CTACAGCGGC 
AGCCAGAGGA GTAGCCGGGG CCGGGGGCCG CGGGAGTTTG GGCCGCCGGA TGACTTCCCC 
CGGGCCAAGC CCCGGCGGAG GATTGCCAGT GAGACCCACA GCGAGGGCTC TGAGTATGAG 
GAGCTCCCCA AGCGGCGGCG GCAGCGAGGC ACTGAGCCTG GGGATGAGGG TGCGCTCCCG 
GAGCGGGAGG AGGCCACGGG AAGGAAGGGC GACTTCAGGG AGTCCTGGCG GTCCGGCAGG 
ATGCACCCTG AGGACCATGG TGGGCCTGAG GCCAAGAACC GAGGTCCCCG GGCCTTCGGG 
CGTGCCCTGC CCCCGCGTCT GAGCAACTGT GCATATGGCC GGAGAACCTT CGCGGCCAAG 
GAGGTGGCCC CTTGGCCCAG CCGGGGTCCT GGCGCCTCCT GGCAGGAGTC TGGCGCCTCT 
GACGCATGTG GAGCCCGGCG GCCCTTAGAC AGAGACTACA TCCCAGACGC CTACAGACGC 
CCCGACGCCT TGGGCTCCCG GGGCTTCGAG GATGGCCGCC TGGAGGACCG GAGGGCCTTC 
TTCCAAGATG ACCACGCCGC AGAGTCCGAA AACGCAGAGA ACCGGCCCTT CCGGCGAAGG 
CGGCCTCCGC GCCAGGACAA GCCGCCCCGC TTCCGGCGCC TCCGGCAGGA ACGGGAGGCC 
CTGGGCCTGT GGGGGCCGGA GGACGAGCCA CCTCTGGCAG CCGGCCAGTG GCCAGGCCGT 
CCTAGACCCT GCCCCGGGGA CAGGAGCAGC GCCTCAGGCC GCAGGTCCCC TGAGCTCGCC 
TATCAGAACT CCTCCGACCA CGCCAACGAG GAGTGGGAGA CGGCCTCCGA GAGCAGTGAC 
TTCAGCGAGA GGCGGGAGCG GCGGGCCGGC TCCGAGCTGG ACCCCCAGGC TGATGGCAGC 
CCCTCTGGGG TGAGCGCAGG CGAGAAGAAG GAGCTGGCCA AGCGGAGCTT CTCGGGCCAG 
AGACCCCTGG TGGACAGGCA GAGCCGGAAG CTGGAGCCGG GAGGGTTTGG GGAGAAGTCT 
GTTAGGCCAG GTGGCGGTGA CACTTCTCCC CGCTATGAGA GTCATCAGAA CGGGACGCCT 
CTGAAAGCCA AAAGGTCCCC AGACGAGGCC TTGCCTGGAG GTCTTGGCTG CGGCAGTGGG 
AGCGGCCATT ACACGCTGGA GCGGGCAGCC CATGCTGCCT CTGACGTCAC CGAAGCCTCC 
TGTGAGAAGG TAGAGAAGGA GGCCAAGCTG GCCGCCCAGA GGTCAGGCGA GCAGGGAGAG 
ACCATGAAAC CATTTGACCT GAACTACGGA CACGCCATCA TTGAAAATTG CGGATCCAGC 
CCTGGGGAGG AGAGTGAGGT GGGCTCTCTG GTGGGCGAAG GCTTCATTGA GGTCCTGACC 
AAGAAGCAGC GCCGCCTGCT GGAGGAGGAG AGGAGAAAGA AGGAGCAAGC CGTGCAGGTT 
CCTGTCAAAG GCCGAAGTCT CTCCTCTCGA ATTCCTCCTC GGTTTGCAAA GAAGCAGAAC 
AACCTGTGCC TGGAGCAGGG TGATGGGCCT GTGCCCGGCA GCAGCCTGGG CACCGAGATC 
TGGGAGAGCG GCAGCCAGGC CCTCCCCCTT CAGGCCTCGA CCAGTGACTC CTGGACCAAA 
GCTGCCACTG CCTTCACCAG CGCTGAGCCT GGCTCTGCTG AGCAGGGCTT TAAGAGCAGC 
CAGGGAGACA GCGGTGTTGA CCTGAGCGCC GAGTCCCGAG AGTCCTCAGC GACCTCCTCG 
CAGCGCAGCT CTCCATATGG CACCCTGAAG CCCGAGGAGG CAAGCGGGCC CGGCCTGGAG 
CCCAAGGCCG ATGGCCACAA GGAGCAGACT CAAAAGCAGT CAGAGCCAAA GGATTCAGAA 
CAAGGCTCAG GACAGAGCAA GGAGCACAGA CCAGGACCCA TTGGCAATGA GCGCTCTCTG 
AAAAACAGAA AGGGCTCCGA GGGGGCTGAA CGGCTGCAGG GGGCTGTGGT TCCCCCTGTT 
AACGGGGTGG AGATTCACGT GGACTCTGTG CTGCCGGTGC CACCCATTGA ATTTGGAGTC 
AATCCGAAGG ACTCTGATTT CAGCTTGCAG CCTGGCTCTG CCTCTGGTCC TGCGGGGAAT 
CCAGTTGTCA AGCTTCAGGA CGCATTGGCC AGTAACGCCG GGCTGGCCCC CAGCATCCCC 
ATCCTGCGGC GGGACCACCA CATCCAGAGG GCCATCGGCC TCTCCCACAT GTCCTTCCCT 
ACCGCCGACC TCACTCTGAA GATGGAGTCT GCGCGCAAGG CTTGGGAAAA CTCGCCCAGC 
TTGCCGGAGC AGAGCTCGCC AGGAGGCGCC GGCTCAGGCC TGCAGCCCCC ATCCTCCGGG 
GGAACCTCCA GCGGGGTCAG CTATAGCTCC TTTGGCGGCG TCTCCATGCC CCCCGTGCCA 
GTGGCCTCGG TCGCACCTTC TGCATCCCTT CCAGGCAGCC ACCTGCCACC TCTCTACCTG 
GATGGCCATG TGTTTGCAAG TCAGCCCCGA CTGGTTCCTC AGACCATACC TCAGCAGCAG 
AGTTTCCAAC AGGCTGCCGC TGCCCAGCAA ATCCCAATTT CCCTTCACAC GTCTCTACAA 
GCTCAAGCCC AGCTGGGACT GAGGGGCGGG CTGCCCGTCT CCCAGTCTCA GGAGATCTTC 
AGCTCCTTAC AGCCCTTCAG GTCTCAGGTG TACATGCACC CCAGCCTGTC ACCGCCCAGC 
ACCATGATCC TCTCCGGAGG CACAGCCTTG AAGGCCCCCT ACTCGGCGTT CCCAGGCATG 
CAGCCCCTGG AGATGGTGAA GCCCCAGTCC GGCTCGCCCT ACCAGCCCAT GAGTGGGAGC 
CAGGCCCTGG TCTATGAGGG CCCGCTCAGC CAGGCGGCTG GGCTTGGCGC CTCCCAGATG 
TTGGACTCCC AGCTCCCGCA GCTGACGATG CCCCTGCCTG GCTCCCAGCT GCCTCTGCCC 
CGGTACGGCT CCGGGCAGCA GCCCCTGCTC CTGCCACAGT CCATCCAGCT GCCCCAGGCG 
CAGAGCCTGT CAGTCGGGGC CCCCCGCAGG ATCCTTGCCC CCGGGTCCCA GCCTTCGGTC 
CTCAACACCA GCAGAGAGTC CTCTCAGATG GAGATGAAGG GCTTCCACTT TGCCGACAGT 
AAACAGAATG TTCCTTCAGG AGGCTCCGTG CCGTCACCAC AGGCCTACAG GCCTAGCTCT 
GCTAGCCCCA GTGGGAAGCC CTCTGGATCA GCAGTTAACA TGGGCTCTGT GCAGGGACAC 
TACGTTCAAC AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGAAGCTG 
CAGGAGCCAC CCTCAGCCGC TTCCCCGCTG AAGCGAACTG GAGCAATCAA GCCCCGGGCG 
GTCAAGGTGG AGGAGAGCAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_024999601.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855369.1 Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.

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CloneID	OBa24521
Clone ID Related Accession (Same CDS sequence)	XM_024999601.1 , XM_005213446.3 , XM_015465516.1
Accession Version	XM_024999601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855369.1
CDS	311..6835
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRQSSEWMKSPAWEP

Target ORF information:

RefSeq Version	XM_024999601.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline rich coiled-coil 2B (PRRC2B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999601.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005213446.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213503.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.

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CloneID	OBa24521
Clone ID Related Accession (Same CDS sequence)	XM_024999601.1 , XM_005213446.3 , XM_015465516.1
Accession Version	XM_005213446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213503.1
CDS	1412..7936
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRQSSEWMKSPAWEP

Target ORF information:

RefSeq Version	XM_005213446.3
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213446.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_015465516.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321002.1 Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.

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CloneID	OBa24521
Clone ID Related Accession (Same CDS sequence)	XM_024999601.1 , XM_005213446.3 , XM_015465516.1
Accession Version	XM_015465516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein PRRC2B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321002.1
CDS	1412..7936
Translation	MSDRLGQITKGKDGKSKYSTLSLFDKYKGKSVDSVRSSVIPRHGLQSLGKVATARRMPPPANLPSLKSENKGNDPNIVIVPKDGTGWANKQDQQDPKSSSVTASQPPELLPQPGLQKSVSNLQKPTQSISQENTNSAPGGPKSWAQLNGKPAGHEGGLRASSRLLSFSPEEFPTLKAAGGQDKAGKEKGVLDPSYGPGPSLRPQNVTSWREGGGRNIISATSLSASPTELGSRNSSAGDGAPSSACASESKDPSLRPPQPVRRGASQFLGSVYHPPTYHDMLPAFMCSPQSSENQGTVERGSFPLPQLRLEPRVPFRQFQMNDQDGKENRLGVSRPIRPLRPLVERVPRPTIVNAENLKGLDDLDTDADDGWAGLHEEVDYSEKLKFSDDEEEEEVLKDGRPKWNSWDPRRQRQLSLSSADSADAKRTQEEGKDWSETGGVTRVVRKVPEPQPPSRKLHSWASGPDYQQKSSMGTVFRQQSAEDKEDKPPPRQKFVQSEMSEAVERARKRREEEERRAREERLAACAAKLKQLDQKCKQAQRASEAQKQAEKEAPRSPGAEKVTPQENGPAVHKGSPEFSAQETASTFSEEAPTAPPAVAQSGGSEEGPREAGSPAQEFNKYQKSLPPRFQRQQQQQQQEQLYKMQHWQPVYPPPSHPQRTFYPHHPQMLGFDPRWMMMPSYMDPRIPPTRTPVDFYPSALHPSGLMKPIMPPDSLSGTGCRSEDQSCVPPLPERKVTAIDPAPVWSPEGYMALQSKGYSLPHPKSSDNMAMDMHVRSESSYSASPGRSGGVSAQRELLEERGQEYLSASDKKAPADCDSRVSSQRVGQELLFPPQENGQEAGTPGSHTPNLRCSPLEPDFAPTEKKLEYGDWDVNHQSKVANPDAAVEKEVPREEPPFSVSSWEKEGSPSKPPALEPEWTPEARGTGSQHPEQTSRTRRSGPIKKPVLKALKVEDKEKELEKVKPELAEGSTRLAREPGPVHEVAEDEDQENDPALANAAPATSEDQGLARASLGREASRPEEDNKPDGAPEARPSREASHTPPTKRNNWIFIDEEQAFGGRGPARGRGRGFREFTFRGRPTGSGLCGGGVLGARGLYSGSQRSSRGRGPREFGPPDDFPRAKPRRRIASETHSEGSEYEELPKRRRQRGTEPGDEGALPEREEATGRKGDFRESWRSGRMHPEDHGGPEAKNRGPRAFGRALPPRLSNCAYGRRTFAAKEVAPWPSRGPGASWQESGASDACGARRPLDRDYIPDAYRRPDALGSRGFEDGRLEDRRAFFQDDHAAESENAENRPFRRRRPPRQDKPPRFRRLRQEREALGLWGPEDEPPLAAGQWPGRPRPCPGDRSSASGRRSPELAYQNSSDHANEEWETASESSDFSERRERRAGSELDPQADGSPSGVSAGEKKELAKRSFSGQRPLVDRQSRKLEPGGFGEKSVRPGGGDTSPRYESHQNGTPLKAKRSPDEALPGGLGCGSGSGHYTLERAAHAASDVTEASCEKVEKEAKLAAQRSGEQGETMKPFDLNYGHAIIENCGSSPGEESEVGSLVGEGFIEVLTKKQRRLLEEERRKKEQAVQVPVKGRSLSSRIPPRFAKKQNNLCLEQGDGPVPGSSLGTEIWESGSQALPLQASTSDSWTKAATAFTSAEPGSAEQGFKSSQGDSGVDLSAESRESSATSSQRSSPYGTLKPEEASGPGLEPKADGHKEQTQKQSEPKDSEQGSGQSKEHRPGPIGNERSLKNRKGSEGAERLQGAVVPPVNGVEIHVDSVLPVPPIEFGVNPKDSDFSLQPGSASGPAGNPVVKLQDALASNAGLAPSIPILRRDHHIQRAIGLSHMSFPTADLTLKMESARKAWENSPSLPEQSSPGGAGSGLQPPSSGGTSSGVSYSSFGGVSMPPVPVASVAPSASLPGSHLPPLYLDGHVFASQPRLVPQTIPQQQSFQQVMPAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIFSSLQPFRSQVYMHPSLSPPSTMILSGGTALKAPYSAFPGMQPLEMVKPQSGSPYQPMSGSQALVYEGPLSQAAGLGASQMLDSQLPQLTMPLPGSQLPLPRYGSGQQPLLLPQSIQLPQAQSLSVGAPRRILAPGSQPSVLNTSRESSQMEMKGFHFADSKQNVPSGGSVPSPQAYRQSSEWMKSPAWEP

Target ORF information:

RefSeq Version	XM_015465516.1
Organism	Bos taurus(cattle)
Definition	Bos taurus proline-rich coiled-coil 2B (PRRC2B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465516.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG GGCAAGGATG GGAAAAGCAA GTATTCGACT 
CTCAGCCTGT TTGACAAGTA TAAAGGAAAA TCAGTAGATT CAGTCAGATC CTCAGTTATT 
CCTCGACATG GCTTACAAAG TCTTGGGAAA GTTGCCACGG CCCGGCGCAT GCCACCACCT 
GCAAACCTGC CAAGCCTGAA GTCTGAAAAC AAAGGAAACG ACCCCAACAT CGTGATAGTA 
CCCAAGGACG GAACAGGATG GGCAAACAAG CAGGACCAGC AAGACCCAAA GAGTTCCAGT 
GTGACGGCCT CTCAGCCGCC GGAGTTGCTG CCGCAGCCGG GTTTGCAGAA ATCTGTCTCC 
AATTTGCAGA AGCCGACACA GTCGATCAGT CAGGAGAATA CAAATTCAGC TCCAGGTGGA 
CCAAAGTCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC ACGAAGGTGG TTTAAGGGCC 
TCAAGCCGAC TGTTATCCTT CTCTCCCGAG GAATTTCCGA CGCTGAAAGC AGCTGGAGGG 
CAGGACAAGG CTGGCAAAGA AAAGGGCGTC TTAGATCCGT CGTATGGGCC AGGACCAAGC 
CTCCGCCCTC AGAATGTGAC AAGCTGGAGG GAGGGCGGTG GGCGAAACAT AATTTCTGCC 
ACGTCTCTGA GCGCCTCCCC AACTGAGCTG GGCAGCAGGA ATTCGAGTGC GGGAGACGGA 
GCCCCCTCCT CGGCGTGCGC CAGCGAGTCT AAGGACCCCT CGCTCCGCCC GCCTCAGCCT 
GTCCGCAGAG GGGCTTCACA GTTCTTGGGG AGTGTATACC ACCCACCTAC ATACCATGAC 
ATGCTTCCTG CCTTTATGTG TTCGCCACAG TCATCAGAGA ACCAGGGTAC AGTGGAACGA 
GGATCTTTTC CCCTTCCTCA GCTCCGTCTT GAACCCCGTG TTCCTTTTAG ACAGTTCCAG 
ATGAATGACC AAGACGGAAA AGAAAATAGA CTGGGAGTGA GCCGCCCGAT CCGTCCACTG 
AGGCCGCTGG TGGAGCGGGT GCCACGACCC ACCATCGTCA ATGCAGAAAA CCTGAAGGGC 
CTCGATGACC TGGACACTGA CGCCGACGAT GGCTGGGCAG GCCTCCATGA AGAAGTGGAC 
TACTCCGAGA AGCTGAAGTT CAGTGATGAT GAGGAGGAGG AGGAAGTGTT GAAGGACGGC 
AGGCCAAAGT GGAACAGTTG GGACCCCAGG AGGCAGCGGC AGTTATCCTT GAGCTCTGCA 
GACAGTGCTG ATGCCAAGCG CACCCAAGAG GAAGGAAAAG ACTGGAGTGA AACAGGAGGC 
GTGACCCGTG TCGTCCGGAA GGTGCCGGAA CCTCAGCCGC CTTCCAGGAA GCTGCACAGC 
TGGGCGTCAG GCCCTGACTA CCAGCAGAAA TCCTCCATGG GCACTGTATT TCGGCAGCAG 
TCTGCCGAGG ACAAAGAGGA CAAGCCCCCA CCGCGGCAGA AGTTTGTCCA GTCTGAGATG 
TCCGAGGCTG TGGAGCGAGC CCGGAAGCGC CGGGAGGAGG AGGAGCGCCG TGCGCGCGAG 
GAAAGGCTGG CTGCCTGTGC TGCCAAACTC AAGCAGCTTG ACCAGAAGTG CAAGCAGGCT 
CAGCGGGCCA GCGAGGCCCA GAAGCAGGCG GAGAAGGAAG CTCCCCGCTC TCCGGGGGCT 
GAGAAGGTGA CTCCCCAGGA GAACGGCCCT GCCGTCCACA AAGGCTCCCC TGAGTTCTCT 
GCCCAGGAGA CCGCCAGCAC GTTTTCAGAA GAAGCCCCCA CAGCTCCTCC AGCAGTGGCT 
CAGAGCGGCG GCAGTGAGGA GGGGCCCCGG GAGGCTGGCT CCCCTGCACA GGAATTCAAC 
AAGTACCAAA AGTCACTCCC TCCCAGATTC CAGCGCCAGC AGCAGCAGCA GCAGCAGGAG 
CAGCTGTACA AGATGCAGCA CTGGCAGCCG GTGTACCCTC CGCCGTCCCA CCCGCAGCGC 
ACCTTCTACC CGCACCACCC CCAGATGCTG GGCTTCGACC CCAGGTGGAT GATGATGCCC 
TCCTACATGG ACCCACGGAT CCCGCCCACT CGGACCCCAG TGGATTTCTA CCCCTCGGCC 
CTGCATCCCT CAGGATTGAT GAAACCCATA ATGCCCCCGG ATTCCCTCAG TGGGACTGGC 
TGTCGCTCTG AGGACCAGAG CTGTGTGCCC CCGCTGCCAG AAAGGAAGGT GACCGCCATT 
GACCCTGCCC CCGTGTGGAG CCCTGAGGGT TACATGGCGT TGCAGAGCAA GGGCTACTCG 
CTGCCCCATC CGAAATCCAG CGACAATATG GCCATGGACA TGCATGTCAG GAGTGAAAGC 
TCTTACTCTG CCTCCCCCGG AAGGTCAGGG GGCGTGAGTG CCCAGCGCGA GCTCCTTGAG 
GAGAGAGGGC AGGAGTACTT GAGCGCTTCT GACAAGAAGG CCCCAGCAGA CTGTGACTCC 
CGTGTCTCCT CTCAGAGAGT AGGCCAGGAG CTTTTGTTTC CACCCCAAGA AAATGGGCAG 
GAAGCAGGCA CTCCTGGGAG TCACACCCCA AACCTCAGGT GCTCCCCACT GGAGCCCGAC 
TTTGCCCCCA CGGAGAAAAA GCTCGAGTAT GGTGACTGGG ATGTGAACCA CCAGTCAAAG 
GTTGCCAACC CAGACGCTGC GGTTGAGAAG GAGGTACCGC GGGAGGAGCC ACCCTTCAGC 
GTCTCCTCCT GGGAGAAGGA AGGGAGCCCG AGCAAGCCGC CGGCTCTGGA GCCCGAGTGG 
ACGCCAGAAG CCCGGGGCAC TGGCAGCCAG CACCCGGAGC AGACCAGCAG GACCCGTAGG 
TCCGGCCCCA TCAAGAAACC TGTCCTGAAG GCCCTCAAGG TGGAGGACAA GGAGAAGGAG 
CTCGAGAAAG TAAAGCCGGA GCTGGCAGAG GGCAGCACCC GCCTGGCCCG GGAGCCAGGG 
CCTGTCCATG AAGTGGCTGA GGACGAGGAC CAAGAGAACG ACCCCGCACT GGCCAATGCG 
GCCCCGGCCA CCTCGGAGGA CCAGGGCTTA GCCCGTGCCA GCTTGGGCCG TGAGGCCAGC 
CGTCCTGAGG AGGACAACAA GCCGGATGGG GCCCCGGAGG CCAGACCCTC CAGGGAGGCC 
AGCCACACAC CCCCGACCAA GAGGAATAAC TGGATCTTTA TTGATGAGGA GCAAGCCTTT 
GGGGGCCGGG GGCCAGCCCG GGGCCGAGGC CGCGGGTTCC GAGAGTTCAC CTTCCGCGGG 
CGGCCCACCG GTAGTGGCCT GTGTGGCGGG GGCGTCCTTG GGGCACGCGG CCTCTACAGC 
GGCAGCCAGA GGAGTAGCCG GGGCCGGGGG CCGCGGGAGT TTGGGCCGCC GGATGACTTC 
CCCCGGGCCA AGCCCCGGCG GAGGATTGCC AGTGAGACCC ACAGCGAGGG CTCTGAGTAT 
GAGGAGCTCC CCAAGCGGCG GCGGCAGCGA GGCACTGAGC CTGGGGATGA GGGTGCGCTC 
CCGGAGCGGG AGGAGGCCAC GGGAAGGAAG GGCGACTTCA GGGAGTCCTG GCGGTCCGGC 
AGGATGCACC CTGAGGACCA TGGTGGGCCT GAGGCCAAGA ACCGAGGTCC CCGGGCCTTC 
GGGCGTGCCC TGCCCCCGCG TCTGAGCAAC TGTGCATATG GCCGGAGAAC CTTCGCGGCC 
AAGGAGGTGG CCCCTTGGCC CAGCCGGGGT CCTGGCGCCT CCTGGCAGGA GTCTGGCGCC 
TCTGACGCAT GTGGAGCCCG GCGGCCCTTA GACAGAGACT ACATCCCAGA CGCCTACAGA 
CGCCCCGACG CCTTGGGCTC CCGGGGCTTC GAGGATGGCC GCCTGGAGGA CCGGAGGGCC 
TTCTTCCAAG ATGACCACGC CGCAGAGTCC GAAAACGCAG AGAACCGGCC CTTCCGGCGA 
AGGCGGCCTC CGCGCCAGGA CAAGCCGCCC CGCTTCCGGC GCCTCCGGCA GGAACGGGAG 
GCCCTGGGCC TGTGGGGGCC GGAGGACGAG CCACCTCTGG CAGCCGGCCA GTGGCCAGGC 
CGTCCTAGAC CCTGCCCCGG GGACAGGAGC AGCGCCTCAG GCCGCAGGTC CCCTGAGCTC 
GCCTATCAGA ACTCCTCCGA CCACGCCAAC GAGGAGTGGG AGACGGCCTC CGAGAGCAGT 
GACTTCAGCG AGAGGCGGGA GCGGCGGGCC GGCTCCGAGC TGGACCCCCA GGCTGATGGC 
AGCCCCTCTG GGGTGAGCGC AGGCGAGAAG AAGGAGCTGG CCAAGCGGAG CTTCTCGGGC 
CAGAGACCCC TGGTGGACAG GCAGAGCCGG AAGCTGGAGC CGGGAGGGTT TGGGGAGAAG 
TCTGTTAGGC CAGGTGGCGG TGACACTTCT CCCCGCTATG AGAGTCATCA GAACGGGACG 
CCTCTGAAAG CCAAAAGGTC CCCAGACGAG GCCTTGCCTG GAGGTCTTGG CTGCGGCAGT 
GGGAGCGGCC ATTACACGCT GGAGCGGGCA GCCCATGCTG CCTCTGACGT CACCGAAGCC 
TCCTGTGAGA AGGTAGAGAA GGAGGCCAAG CTGGCCGCCC AGAGGTCAGG CGAGCAGGGA 
GAGACCATGA AACCATTTGA CCTGAACTAC GGACACGCCA TCATTGAAAA TTGCGGATCC 
AGCCCTGGGG AGGAGAGTGA GGTGGGCTCT CTGGTGGGCG AAGGCTTCAT TGAGGTCCTG 
ACCAAGAAGC AGCGCCGCCT GCTGGAGGAG GAGAGGAGAA AGAAGGAGCA AGCCGTGCAG 
GTTCCTGTCA AAGGCCGAAG TCTCTCCTCT CGAATTCCTC CTCGGTTTGC AAAGAAGCAG 
AACAACCTGT GCCTGGAGCA GGGTGATGGG CCTGTGCCCG GCAGCAGCCT GGGCACCGAG 
ATCTGGGAGA GCGGCAGCCA GGCCCTCCCC CTTCAGGCCT CGACCAGTGA CTCCTGGACC 
AAAGCTGCCA CTGCCTTCAC CAGCGCTGAG CCTGGCTCTG CTGAGCAGGG CTTTAAGAGC 
AGCCAGGGAG ACAGCGGTGT TGACCTGAGC GCCGAGTCCC GAGAGTCCTC AGCGACCTCC 
TCGCAGCGCA GCTCTCCATA TGGCACCCTG AAGCCCGAGG AGGCAAGCGG GCCCGGCCTG 
GAGCCCAAGG CCGATGGCCA CAAGGAGCAG ACTCAAAAGC AGTCAGAGCC AAAGGATTCA 
GAACAAGGCT CAGGACAGAG CAAGGAGCAC AGACCAGGAC CCATTGGCAA TGAGCGCTCT 
CTGAAAAACA GAAAGGGCTC CGAGGGGGCT GAACGGCTGC AGGGGGCTGT GGTTCCCCCT 
GTTAACGGGG TGGAGATTCA CGTGGACTCT GTGCTGCCGG TGCCACCCAT TGAATTTGGA 
GTCAATCCGA AGGACTCTGA TTTCAGCTTG CAGCCTGGCT CTGCCTCTGG TCCTGCGGGG 
AATCCAGTTG TCAAGCTTCA GGACGCATTG GCCAGTAACG CCGGGCTGGC CCCCAGCATC 
CCCATCCTGC GGCGGGACCA CCACATCCAG AGGGCCATCG GCCTCTCCCA CATGTCCTTC 
CCTACCGCCG ACCTCACTCT GAAGATGGAG TCTGCGCGCA AGGCTTGGGA AAACTCGCCC 
AGCTTGCCGG AGCAGAGCTC GCCAGGAGGC GCCGGCTCAG GCCTGCAGCC CCCATCCTCC 
GGGGGAACCT CCAGCGGGGT CAGCTATAGC TCCTTTGGCG GCGTCTCCAT GCCCCCCGTG 
CCAGTGGCCT CGGTCGCACC TTCTGCATCC CTTCCAGGCA GCCACCTGCC ACCTCTCTAC 
CTGGATGGCC ATGTGTTTGC AAGTCAGCCC CGACTGGTTC CTCAGACCAT ACCTCAGCAG 
CAGAGTTTCC AACAGGTGAT GCCGGCTGCC GCTGCCCAGC AAATCCCAAT TTCCCTTCAC 
ACGTCTCTAC AAGCTCAAGC CCAGCTGGGA CTGAGGGGCG GGCTGCCCGT CTCCCAGTCT 
CAGGAGATCT TCAGCTCCTT ACAGCCCTTC AGGTCTCAGG TGTACATGCA CCCCAGCCTG 
TCACCGCCCA GCACCATGAT CCTCTCCGGA GGCACAGCCT TGAAGGCCCC CTACTCGGCG 
TTCCCAGGCA TGCAGCCCCT GGAGATGGTG AAGCCCCAGT CCGGCTCGCC CTACCAGCCC 
ATGAGTGGGA GCCAGGCCCT GGTCTATGAG GGCCCGCTCA GCCAGGCGGC TGGGCTTGGC 
GCCTCCCAGA TGTTGGACTC CCAGCTCCCG CAGCTGACGA TGCCCCTGCC TGGCTCCCAG 
CTGCCTCTGC CCCGGTACGG CTCCGGGCAG CAGCCCCTGC TCCTGCCACA GTCCATCCAG 
CTGCCCCAGG CGCAGAGCCT GTCAGTCGGG GCCCCCCGCA GGATCCTTGC CCCCGGGTCC 
CAGCCTTCGG TCCTCAACAC CAGCAGAGAG TCCTCTCAGA TGGAGATGAA GGGCTTCCAC 
TTTGCCGACA GTAAACAGAA TGTTCCTTCA GGAGGCTCCG TGCCGTCACC ACAGGCCTAC 
AGGCAAAGCA GCGAGTGGAT GAAAAGCCCG GCCTGGGAGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PRRC2B
Entrez Gene ID	505073
Full Name	proline rich coiled-coil 2B
Synonyms	BAT2L,BAT2L1
General protein information	Preferred Names proline rich coiled-coil 2B Names protein PRRC2B HLA-B associated transcript 2-like 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Danio rerio (zebrafish)	LOC561451	XP_005172099.1
Xenopus tropicalis (tropical clawed frog)	prrc2b	XP_002936236.2
Homo sapiens (human)	PRRC2B	NP_037450.2
Mus musculus (house mouse)	Prrc2b	NP_001153106.1
	LOC100425699	XP_002800001.1
Canis lupus familiaris (dog)	PRRC2B	XP_548409.3
Rattus norvegicus (Norway rat)	Prrc2b	XP_006234067.1
Pan troglodytes (chimpanzee)	PRRC2B	XP_520327.2
Bos taurus (cattle)	PRRC2B	XP_005213498.1
Gallus gallus (chicken)	PRRC2B	XP_415459.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PRRC2B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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