AFDN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFDN
Entrez Gene ID	504856
Full Name	afadin, adherens junction formation factor
General protein information	Preferred Names afadin, adherens junction formation factor Names afadin myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015464902.1	XP_015320388.1	afadin isoform X20
XM_015472946.1	XP_015328432.1	afadin isoform X9
XM_015472948.1	XP_015328434.1	afadin isoform X11
XM_010808750.2	XP_010807052.1	afadin isoform X6
XM_024997138.1	XP_024852906.1	afadin isoform X3
XM_024997142.1	XP_024852910.1	afadin isoform X7
XM_024997144.1	XP_024852912.1	afadin isoform X9
XM_024997145.1	XP_024852913.1	afadin isoform X10
XM_024997149.1	XP_024852917.1	afadin isoform X14
XM_015464901.1	XP_015320387.1	afadin isoform X19
XM_015464903.1	XP_015320389.1	afadin isoform X21
XM_015464909.1	XP_015320395.1	afadin isoform X27
XM_015472941.1	XP_015328427.1	afadin isoform X2
XM_015472942.1	XP_015328428.1	afadin isoform X3
XM_024997139.1	XP_024852907.1	afadin isoform X4
XM_024997146.1	XP_024852914.1	afadin isoform X11
XM_024997152.1	XP_024852920.1	afadin isoform X17
XM_024997154.1	XP_024852922.1	afadin isoform X19
XM_010808755.2	XP_010807057.1	afadin isoform X12
XM_010808758.2	XP_010807060.1	afadin isoform X15
XM_015472944.1	XP_015328430.1	afadin isoform X5
XM_015464898.1	XP_015320384.1	afadin isoform X16
XM_015464906.1	XP_015320392.1	afadin isoform X24
XM_015464907.1	XP_015320393.1	afadin isoform X25
XM_024997137.1	XP_024852905.1	afadin isoform X2
XM_024997150.1	XP_024852918.1	afadin isoform X15
XM_024997151.1	XP_024852919.1	afadin isoform X16
XM_015464899.1	XP_015320385.1	afadin isoform X17
XM_015464904.1	XP_015320390.1	afadin isoform X22
XM_015472943.1	XP_015328429.1	afadin isoform X4
XM_010808751.2	XP_010807053.1	afadin isoform X7
XM_010808756.2	XP_010807058.1	afadin isoform X13
XM_010808757.2	XP_010807059.1	afadin isoform X14
XM_024997136.1	XP_024852904.1	afadin isoform X1
XM_024997140.1	XP_024852908.1	afadin isoform X5
XM_024997143.1	XP_024852911.1	afadin isoform X8
XM_024997147.1	XP_024852915.1	afadin isoform X12
XM_024997148.1	XP_024852916.1	afadin isoform X13
XM_024997153.1	XP_024852921.1	afadin isoform X18
XM_015464900.1	XP_015320386.1	afadin isoform X18
XM_015464905.1	XP_015320391.1	afadin isoform X23
XM_015464908.1	XP_015320394.1	afadin isoform X26
XM_015472945.1	XP_015328431.1	afadin isoform X8
XM_015472947.1	XP_015328433.1	afadin isoform X10
XM_010808749.2	XP_010807051.1	afadin isoform X1
XM_024997141.1	XP_024852909.1	afadin isoform X6

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	afd-1	NP_001021660.1
Gallus gallus (chicken)	MLLT4	XP_004935593.1
Danio rerio (zebrafish)	mllt4	XP_686655.5
Homo sapiens (human)	MLLT4	NP_001193937.1
Pan troglodytes (chimpanzee)	MLLT4	XP_518857.4
	MLLT4	XP_001083153.1
Canis lupus familiaris (dog)	MLLT4	XP_005615632.1
Bos taurus (cattle)	MLLT4	XP_005211198.1
Mus musculus (house mouse)	Mllt4	NP_034936.1
Rattus norvegicus (Norway rat)	Mllt4	NP_037349.1
Xenopus tropicalis (tropical clawed frog)	mllt4	XP_002931548.2

Gene Ontology

The following AFDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238187	XM_024997152.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa238174	XM_024997136.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238175	XM_024997137.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238176	XM_024997138.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238177	XM_024997139.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238178	XM_024997140.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238179	XM_024997141.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238180	XM_024997142.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238181	XM_024997143.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238182	XM_024997144.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238183	XM_024997148.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238184	XM_024997149.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238185	XM_024997150.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa238186	XM_024997151.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa06241	XM_010808756.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa46103	XM_010808749.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa46104	XM_010808750.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa46105	XM_010808751.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa46109	XM_010808755.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa46110	XM_010808757.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa46111	XM_010808758.2 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa91541	XM_015472941.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa91542	XM_015472942.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa91543	XM_015472943.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa91544	XM_015472944.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa91545	XM_015472945.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa91546	XM_015472946.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa91547	XM_015472947.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa91548	XM_015472948.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa94637	XM_024997145.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94637	XM_015464898.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94638	XM_015464899.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94639	XM_015464900.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94640	XM_015464901.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94641	XM_015464902.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94642	XM_024997146.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94642	XM_015464903.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94643	XM_015464904.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94643	XM_024997147.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94644	XM_015464905.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94645	XM_015464906.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa94646	XM_015464907.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94647	XM_024997153.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94647	XM_015464908.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa94648	XM_015464909.1 Latest version!	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa94648	XM_024997154.1 Latest version!	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFDN ( XM_024997152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852920.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

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CloneID	OBa238187
Clone ID Related Accession (Same CDS sequence)	XM_024997152.1
Accession Version	XM_024997152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCGA CCCCAGGGAG AAGGAACCAC TCGGCCTGCT ACAGCTCTCA CGCTTCCGAA 
GACGGTTCTG ACTCCAGAGA CAAGCCAAAG CTCTACCGCC TTCAGCTCAG CGTCACGGAG 
GTGGGGACCG AGAAGTTCGA CGACAGCTCC ATCCAGTTGT TTGGTTCAGG CATTCAGCCC 
CATCACTGTG ACCTCACGAA TATGGACGGG GTGGTCACGG TCACGCCGAG AAGCATGGAC 
GCTGAGACCT ACGTGGATGG GCAGCGCATC TCGGAGACCA CGATGCTGCA GAGCGGGATG 
AAGGTGCAGT TCGGGGCGTC CCACGTGTTC AAGTTCGTGG ACCCCAGCCA GGACCACACG 
CTCCCCAAGA GATCCGTGGA CGGGGGCCTG ACTGTGAAGG GCCCCAGACA TAAGTCTGGA 
ATTGTTCAGG AGACAACTTT TGATTTAGGA GGAGACGTTC ACAGTGGAAT GGCCTTACCA 
GCGAGCAAGA GCACCGCCAG GCTGGACAGC GACAGGACGC CGTCCACCTC CAGCACCGCC 
GAGCGGGGGA TGGTGAAGCC GATGGTCCGC GTGGAGCAGC AGCTGGACTA CCGCCGGCAG 
GACAGCAGAA CTCAGGATGC TCCCGGGCCT GACCTGATTC TGCCTGCAAG CATTGAATTC 
AGGGAAAGTT CTGAAGATTC CTTTTTATCT GCCATTATAA ATTACACTAA TAGCTCTACA 
GTCCATTTTA AGTTGTCGCC CACTTACGTG TTATACATGG CCTGTCGGTA TGTATTGTCC 
AGCCAGTACA GACCCGACGT CAGCCCCGCG GAACGCACGC ACAAAGTCAT GGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGGGTG ATCCAGGAAG TAGACCAGGT TGATCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAGTGAGGGA 
GCCTGTGGCC CTTCCCAAGG CCCGCCCCAC ACAGCTGCTC ACCACCGGGG TGGGGGCCGC 
TCGGTCAATA AGGAATGTTC TCGCCTGCGT AGATTAGCCA ACTGGGATCA GCCTCCCAGT 
CCTGGCGGGA AGAGTGCGTA TGCCTCCGGA ACAGCAGCCA AGATCACATC TGTCTCCACG 
GGGAACCTCT GCGCTGAGGA GCAGACGCCT CCGCCCCGCC CCGAAGCCTA CCCCATCCCC 
ACGCAGACGT CCACCAGGGA GTACTTCACC TTCCCGGCCT CCAAGTCCCA GGACCGCGTG 
GCGCCCCCGC AGAGCCAGTG GCTGGGTTTC GAGGAGAAGC CCCACGTGCT GGCAGACGGA 
AACCACTCCA GCGCTGCCGT CCAGCGTGTG ACCCGCTCCC AGGAGGAGCT CCGCGAGGAC 
GCCTACCACC CCGAGCGGCA GCGGCCGGAG GCAGCCGCGG ATCGCAGGTC GGATGGCAGC 
GACGCCTGGC CCACCCAGGG CTCATCACTG GGTTCCGGCG CGTCCAGCCA GGAGCACCTG 
GGGCCCCCCT CCAAGGCGGG CACCCCCGCC TCCACGCTCA CCAAGAGCGG CCCCGGGCGC 
TGGAAGACCC CGGCCGCCTC CCCGCCCATG CGGACCGACC CGGCCCTGCC GCCACCGCCG 
CCCCCCGGGT CCTACGCCGG GGAGGCTGAC GCGGACGCGG ACTCGCCCCC GCCTCCCGCC 
GCCACCGCCG CCGGCCAGGC GCAGGCGGCC GCCGAGCGCA AGAAACGAGA GGAGCAGCAG 
CGCTGGTACG AGAAGGAAAA AGCGCGCCTG GACGAGGAGC GCGAGCGCCG GCGCCGGGAG 
CAGGAGCGCC GGCTGGGCCA GCTGCGCGCG CCCGCGCCGC CCCCCGCGCC CGCCCGCGCG 
CCCGACACGG TGATCCGCGA GCTGCAGCCG CAGCAGCAGC CGCGTACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACGGT CAGCAGGGAG GAGCTGGCCT CCGGAGACAG CCTGTCCCCC 
GACCCCTGGA AGCGCGATGC GCGCGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTG 
GACCTGCTGA GCCGCGAGAT CCAGGAGCTG CAGGGCAAGG CCGAGCGCTC GGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT GCTGCTGGAG TGGCAGTTCC AGAAGCGGCT GCAGGAGTCC 
AAGCAGAAGG ACGAGGACGA CGAGGAGGAG GAGGACGATG ACGTGGACAC CGTGCTGCTC 
ATGCAGCGCC TGGAGGCCGA GCGCAGAGCG CGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGATGA GGAGCGGCGG CGGCAGCAGC AGCTGGAGGA GATGCGGACA 
CGGGAGGCGG AGGAGCGCGC GCGGCAGGAG GAGCAGCGGC GCCGGCAGGA GGACGAGCGG 
GTCAGGCGCG AGGCCGAAGA CAAGAGGCGG CAGGAGGACG GCTACTACAG CCGCCTGGAG 
GCCGAGCGGC GCCGACAGCA TGAGGAGGCC GAGCGCCGGC TGCTGGAGCC CGAGGAGCCG 
GGCCTGGGCC GCCCGCCACT CCCGCGCGGC TATGAGCCCG CCGGCCCGCC GCCACCGCCC 
CAGCGCACCG CCTCCTACCT GCAGACGCAG GCCTTCTCCC CGGACGCCCT CTACACCGCC 
AAGTTCGTGG CCTACAATGA GGAGGAGGAC GAGGACGAAG AGCCGGCAGG AGCAAACTCT 
TACGTGGGAC CTGCTGGGAC AGTTTTTGGA GCCCATGATG TTTATCGGGA CCCGAGAGAG 
AGACCGCCTA AGAGCCACGA AGCAGATGTC CCCGGGGGTC CCGGAGCCCC TGAAAACCTG 
ACGTTCAGGG AGCGCCAGCG CCTTTTCTCC CAAGGCCACG ATGTCTCCAA TAAAGTCAAG 
GCCTCTCGCA AGTTAACAGA GCTGGAGAAC GAGCTGAACA CGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852920.1
CDS	17..4384
Translation	MTPTPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997152.1

ORF Insert Sequence:

ATGACTCCGA CCCCAGGGAG AAGGAACCAC TCGGCCTGCT ACAGCTCTCA CGCTTCCGAA 
GACGGTTCTG ACTCCAGAGA CAAGCCAAAG CTCTACCGCC TTCAGCTCAG CGTCACGGAG 
GTGGGGACCG AGAAGTTCGA CGACAGCTCC ATCCAGTTGT TTGGTTCAGG CATTCAGCCC 
CATCACTGTG ACCTCACGAA TATGGACGGG GTGGTCACGG TCACGCCGAG AAGCATGGAC 
GCTGAGACCT ACGTGGATGG GCAGCGCATC TCGGAGACCA CGATGCTGCA GAGCGGGATG 
AAGGTGCAGT TCGGGGCGTC CCACGTGTTC AAGTTCGTGG ACCCCAGCCA GGACCACACG 
CTCCCCAAGA GATCCGTGGA CGGGGGCCTG ACTGTGAAGG GCCCCAGACA TAAGTCTGGA 
ATTGTTCAGG AGACAACTTT TGATTTAGGA GGAGACGTTC ACAGTGGAAT GGCCTTACCA 
GCGAGCAAGA GCACCGCCAG GCTGGACAGC GACAGGACGC CGTCCACCTC CAGCACCGCC 
GAGCGGGGGA TGGTGAAGCC GATGGTCCGC GTGGAGCAGC AGCTGGACTA CCGCCGGCAG 
GACAGCAGAA CTCAGGATGC TCCCGGGCCT GACCTGATTC TGCCTGCAAG CATTGAATTC 
AGGGAAAGTT CTGAAGATTC CTTTTTATCT GCCATTATAA ATTACACTAA TAGCTCTACA 
GTCCATTTTA AGTTGTCGCC CACTTACGTG TTATACATGG CCTGTCGGTA TGTATTGTCC 
AGCCAGTACA GACCCGACGT CAGCCCCGCG GAACGCACGC ACAAAGTCAT GGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGGGTG ATCCAGGAAG TAGACCAGGT TGATCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAGTGAGGGA 
GCCTGTGGCC CTTCCCAAGG CCCGCCCCAC ACAGCTGCTC ACCACCGGGG TGGGGGCCGC 
TCGGTCAATA AGGAATGTTC TCGCCTGCGT AGATTAGCCA ACTGGGATCA GCCTCCCAGT 
CCTGGCGGGA AGAGTGCGTA TGCCTCCGGA ACAGCAGCCA AGATCACATC TGTCTCCACG 
GGGAACCTCT GCGCTGAGGA GCAGACGCCT CCGCCCCGCC CCGAAGCCTA CCCCATCCCC 
ACGCAGACGT CCACCAGGGA GTACTTCACC TTCCCGGCCT CCAAGTCCCA GGACCGCGTG 
GCGCCCCCGC AGAGCCAGTG GCTGGGTTTC GAGGAGAAGC CCCACGTGCT GGCAGACGGA 
AACCACTCCA GCGCTGCCGT CCAGCGTGTG ACCCGCTCCC AGGAGGAGCT CCGCGAGGAC 
GCCTACCACC CCGAGCGGCA GCGGCCGGAG GCAGCCGCGG ATCGCAGGTC GGATGGCAGC 
GACGCCTGGC CCACCCAGGG CTCATCACTG GGTTCCGGCG CGTCCAGCCA GGAGCACCTG 
GGGCCCCCCT CCAAGGCGGG CACCCCCGCC TCCACGCTCA CCAAGAGCGG CCCCGGGCGC 
TGGAAGACCC CGGCCGCCTC CCCGCCCATG CGGACCGACC CGGCCCTGCC GCCACCGCCG 
CCCCCCGGGT CCTACGCCGG GGAGGCTGAC GCGGACGCGG ACTCGCCCCC GCCTCCCGCC 
GCCACCGCCG CCGGCCAGGC GCAGGCGGCC GCCGAGCGCA AGAAACGAGA GGAGCAGCAG 
CGCTGGTACG AGAAGGAAAA AGCGCGCCTG GACGAGGAGC GCGAGCGCCG GCGCCGGGAG 
CAGGAGCGCC GGCTGGGCCA GCTGCGCGCG CCCGCGCCGC CCCCCGCGCC CGCCCGCGCG 
CCCGACACGG TGATCCGCGA GCTGCAGCCG CAGCAGCAGC CGCGTACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACGGT CAGCAGGGAG GAGCTGGCCT CCGGAGACAG CCTGTCCCCC 
GACCCCTGGA AGCGCGATGC GCGCGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTG 
GACCTGCTGA GCCGCGAGAT CCAGGAGCTG CAGGGCAAGG CCGAGCGCTC GGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT GCTGCTGGAG TGGCAGTTCC AGAAGCGGCT GCAGGAGTCC 
AAGCAGAAGG ACGAGGACGA CGAGGAGGAG GAGGACGATG ACGTGGACAC CGTGCTGCTC 
ATGCAGCGCC TGGAGGCCGA GCGCAGAGCG CGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGATGA GGAGCGGCGG CGGCAGCAGC AGCTGGAGGA GATGCGGACA 
CGGGAGGCGG AGGAGCGCGC GCGGCAGGAG GAGCAGCGGC GCCGGCAGGA GGACGAGCGG 
GTCAGGCGCG AGGCCGAAGA CAAGAGGCGG CAGGAGGACG GCTACTACAG CCGCCTGGAG 
GCCGAGCGGC GCCGACAGCA TGAGGAGGCC GAGCGCCGGC TGCTGGAGCC CGAGGAGCCG 
GGCCTGGGCC GCCCGCCACT CCCGCGCGGC TATGAGCCCG CCGGCCCGCC GCCACCGCCC 
CAGCGCACCG CCTCCTACCT GCAGACGCAG GCCTTCTCCC CGGACGCCCT CTACACCGCC 
AAGTTCGTGG CCTACAATGA GGAGGAGGAC GAGGACGAAG AGCCGGCAGG AGCAAACTCT 
TACGTGGGAC CTGCTGGGAC AGTTTTTGGA GCCCATGATG TTTATCGGGA CCCGAGAGAG 
AGACCGCCTA AGAGCCACGA AGCAGATGTC CCCGGGGGTC CCGGAGCCCC TGAAAACCTG 
ACGTTCAGGG AGCGCCAGCG CCTTTTCTCC CAAGGCCACG ATGTCTCCAA TAAAGTCAAG 
GCCTCTCGCA AGTTAACAGA GCTGGAGAAC GAGCTGAACA CGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997136.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852904.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

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CloneID	OBa238174
Clone ID Related Accession (Same CDS sequence)	XM_024997136.1
Accession Version	XM_024997136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGATGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGACAC GGGAGGCGGA GGAGCGCGCG 
CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG GACGAGCGGG TCAGGCGCGA GGCCGAAGAC 
AAGAGGCGGC AGGAGGACGG CTACTACAGC CGCCTGGAGG CCGAGCGGCG CCGACAGCAT 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCGCCACTC 
CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG CCACCGCCCC AGCGCACCGC CTCCTACCTG 
CAGACGCAGG CCTTCTCCCC GGACGCCCTC TACACCGCCA AGTTCGTGGC CTACAATGAG 
GAGGAGGACG AGGACGAAGA GCCGGCAGGA GCAAACTCTT ACGTGGGACC TGCTGGGACA 
GTTTTTGGAG CCCATGATGT TTATCGGGAC CCGAGAGAGA GACCGCCTAA GAGCCACGAA 
GCAGATGTCC CCGGGGGTCC CGGAGCCCCT GAAAACCTGA CGTTCAGGGA GCGCCAGCGC 
CTTTTCTCCC AAGGCCACGA TGTCTCCAAT AAAGTCAAGG CCTCTCGCAA GTTAACAGAG 
CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852904.1
CDS	71..5557
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997136.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997136.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGATGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGACAC GGGAGGCGGA GGAGCGCGCG 
CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG GACGAGCGGG TCAGGCGCGA GGCCGAAGAC 
AAGAGGCGGC AGGAGGACGG CTACTACAGC CGCCTGGAGG CCGAGCGGCG CCGACAGCAT 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCGCCACTC 
CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG CCACCGCCCC AGCGCACCGC CTCCTACCTG 
CAGACGCAGG CCTTCTCCCC GGACGCCCTC TACACCGCCA AGTTCGTGGC CTACAATGAG 
GAGGAGGACG AGGACGAAGA GCCGGCAGGA GCAAACTCTT ACGTGGGACC TGCTGGGACA 
GTTTTTGGAG CCCATGATGT TTATCGGGAC CCGAGAGAGA GACCGCCTAA GAGCCACGAA 
GCAGATGTCC CCGGGGGTCC CGGAGCCCCT GAAAACCTGA CGTTCAGGGA GCGCCAGCGC 
CTTTTCTCCC AAGGCCACGA TGTCTCCAAT AAAGTCAAGG CCTCTCGCAA GTTAACAGAG 
CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997137.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852905.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OBa238175
Clone ID Related Accession (Same CDS sequence)	XM_024997137.1
Accession Version	XM_024997137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAGT GAGGGAGCCT GTGGCCCTTC CCAAGGCCCG 
CCCCACACAG CTGCTCACCA CCGGGGTGGG GGCCGCTCGG TCAATAAGGA ATGTTCTCGC 
CTGCGTAGAT TAGCCAACTG GGATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852905.1
CDS	72..5555
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997137.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997137.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAGT GAGGGAGCCT GTGGCCCTTC CCAAGGCCCG 
CCCCACACAG CTGCTCACCA CCGGGGTGGG GGCCGCTCGG TCAATAAGGA ATGTTCTCGC 
CTGCGTAGAT TAGCCAACTG GGATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997138.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852906.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OBa238176
Clone ID Related Accession (Same CDS sequence)	XM_024997138.1
Accession Version	XM_024997138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAGT GAGGGAGCCT GTGGCCCTTC CCAAGGCCCG 
CCCCACACAG CTGCTCACCA CCGGGGTGGG GGCCGCTCGG TCAATAAGGA ATGTTCTCGC 
CTGCGTAGAT TAGCCAACTG GGATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852906.1
CDS	72..5555
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997138.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997138.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAGT GAGGGAGCCT GTGGCCCTTC CCAAGGCCCG 
CCCCACACAG CTGCTCACCA CCGGGGTGGG GGCCGCTCGG TCAATAAGGA ATGTTCTCGC 
CTGCGTAGAT TAGCCAACTG GGATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997139.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852907.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OBa238177
Clone ID Related Accession (Same CDS sequence)	XM_024997139.1
Accession Version	XM_024997139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGGA TGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG ACACGGGAGG CGGAGGAGCG CGCGCGGCAG 
GAGGAGCAGC GGCGCCGGCA GGAGGACGAG CGGGTCAGGC GCGAGGCCGA AGACAAGAGG 
CGGCAGGAGG ACGGCTACTA CAGCCGCCTG GAGGCCGAGC GGCGCCGACA GCATGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC 
GGCTATGAGC CCGCCGGCCC GCCGCCACCG CCCCAGCGCA CCGCCTCCTA CCTGCAGACG 
CAGGCCTTCT CCCCGGACGC CCTCTACACC GCCAAGTTCG TGGCCTACAA TGAGGAGGAG 
GACGAGGACG AAGAGCCGGC AGGAGCAAAC TCTTACGTGG GACCTGCTGG GACAGTTTTT 
GGAGCCCATG ATGTTTATCG GGACCCGAGA GAGAGACCGC CTAAGAGCCA CGAAGCAGAT 
GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC CTGACGTTCA GGGAGCGCCA GCGCCTTTTC 
TCCCAAGGCC ACGATGTCTC CAATAAAGTC AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG 
AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852907.1
CDS	71..5551
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997139.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997139.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGGA TGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG ACACGGGAGG CGGAGGAGCG CGCGCGGCAG 
GAGGAGCAGC GGCGCCGGCA GGAGGACGAG CGGGTCAGGC GCGAGGCCGA AGACAAGAGG 
CGGCAGGAGG ACGGCTACTA CAGCCGCCTG GAGGCCGAGC GGCGCCGACA GCATGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC 
GGCTATGAGC CCGCCGGCCC GCCGCCACCG CCCCAGCGCA CCGCCTCCTA CCTGCAGACG 
CAGGCCTTCT CCCCGGACGC CCTCTACACC GCCAAGTTCG TGGCCTACAA TGAGGAGGAG 
GACGAGGACG AAGAGCCGGC AGGAGCAAAC TCTTACGTGG GACCTGCTGG GACAGTTTTT 
GGAGCCCATG ATGTTTATCG GGACCCGAGA GAGAGACCGC CTAAGAGCCA CGAAGCAGAT 
GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC CTGACGTTCA GGGAGCGCCA GCGCCTTTTC 
TCCCAAGGCC ACGATGTCTC CAATAAAGTC AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG 
AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997140.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852908.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

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CloneID	OBa238178
Clone ID Related Accession (Same CDS sequence)	XM_024997140.1
Accession Version	XM_024997140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAGTGAG GGAGCCTGTG GCCCTTCCCA AGGCCCGCCC 
CACACAGCTG CTCACCACCG GGGTGGGGGC CGCTCGGTCA ATAAGGAATG TTCTCGCCTG 
CGTAGATTAG CCAACTGGGA TCAGCCTCCC AGTCCTGGCG GGAAGAGTGC GTATGCCTCC 
GGAACAGCAG CCAAGATCAC ATCTGTCTCC ACGGGGAACC TCTGCGCTGA GGAGCAGACG 
CCTCCGCCCC GCCCCGAAGC CTACCCCATC CCCACGCAGA CGTCCACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAAGTC CCAGGACCGC GTGGCGCCCC CGCAGAGCCA GTGGCTGGGT 
TTCGAGGAGA AGCCCCACGT GCTGGCAGAC GGAAACCACT CCAGCGCTGC CGTCCAGCGT 
GTGACCCGCT CCCAGGAGGA GCTCCGCGAG GACGCCTACC ACCCCGAGCG GCAGCGGCCG 
GAGGCAGCCG CGGATCGCAG GTCGGATGGC AGCGACGCCT GGCCCACCCA GGGCTCATCA 
CTGGGTTCCG GCGCGTCCAG CCAGGAGCAC CTGGGGCCCC CCTCCAAGGC GGGCACCCCC 
GCCTCCACGC TCACCAAGAG CGGCCCCGGG CGCTGGAAGA CCCCGGCCGC CTCCCCGCCC 
ATGCGGACCG ACCCGGCCCT GCCGCCACCG CCGCCCCCCG GGTCCTACGC CGGGGAGGCT 
GACGCGGACG CGGACTCGCC CCCGCCTCCC GCCGCCACCG CCGCCGGCCA GGCGCAGGCG 
GCCGCCGAGC GCAAGAAACG AGAGGAGCAG CAGCGCTGGT ACGAGAAGGA AAAAGCGCGC 
CTGGACGAGG AGCGCGAGCG CCGGCGCCGG GAGCAGGAGC GCCGGCTGGG CCAGCTGCGC 
GCGCCCGCGC CGCCCCCCGC GCCCGCCCGC GCGCCCGACA CGGTGATCCG CGAGCTGCAG 
CCGCAGCAGC AGCCGCGTAC CATCGAGCGC AGGGACCTGC AGTACATCAC GGTCAGCAGG 
GAGGAGCTGG CCTCCGGAGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA TGCGCGCGAG 
AAGTTGGAGA AGCAGCAGCA GCTGCACATC GTGGACCTGC TGAGCCGCGA GATCCAGGAG 
CTGCAGGGCA AGGCCGAGCG CTCGGCCGAG GAGAGCGACC GGCTGCGCAA GCTGCTGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGAGGAG 
GAGGAGGACG ATGACGTGGA CACCGTGCTG CTCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGTTGCAGGA TGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG ACACGGGAGG CGGAGGAGCG CGCGCGGCAG 
GAGGAGCAGC GGCGCCGGCA GGAGGACGAG CGGGTCAGGC GCGAGGCCGA AGACAAGAGG 
CGGCAGGAGG ACGGCTACTA CAGCCGCCTG GAGGCCGAGC GGCGCCGACA GCATGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC 
GGCTATGAGC CCGCCGGCCC GCCGCCACCG CCCCAGCGCA CCGCCTCCTA CCTGCAGACG 
CAGGCCTTCT CCCCGGACGC CCTCTACACC GCCAAGTTCG TGGCCTACAA TGAGGAGGAG 
GACGAGGACG AAGAGCCGGC AGGAGCAAAC TCTTACGTGG GACCTGCTGG GACAGTTTTT 
GGAGCCCATG ATGTTTATCG GGACCCGAGA GAGAGACCGC CTAAGAGCCA CGAAGCAGAT 
GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC CTGACGTTCA GGGAGCGCCA GCGCCTTTTC 
TCCCAAGGCC ACGATGTCTC CAATAAAGTC AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG 
AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852908.1
CDS	72..5552
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997140.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997140.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAGTGAG GGAGCCTGTG GCCCTTCCCA AGGCCCGCCC 
CACACAGCTG CTCACCACCG GGGTGGGGGC CGCTCGGTCA ATAAGGAATG TTCTCGCCTG 
CGTAGATTAG CCAACTGGGA TCAGCCTCCC AGTCCTGGCG GGAAGAGTGC GTATGCCTCC 
GGAACAGCAG CCAAGATCAC ATCTGTCTCC ACGGGGAACC TCTGCGCTGA GGAGCAGACG 
CCTCCGCCCC GCCCCGAAGC CTACCCCATC CCCACGCAGA CGTCCACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAAGTC CCAGGACCGC GTGGCGCCCC CGCAGAGCCA GTGGCTGGGT 
TTCGAGGAGA AGCCCCACGT GCTGGCAGAC GGAAACCACT CCAGCGCTGC CGTCCAGCGT 
GTGACCCGCT CCCAGGAGGA GCTCCGCGAG GACGCCTACC ACCCCGAGCG GCAGCGGCCG 
GAGGCAGCCG CGGATCGCAG GTCGGATGGC AGCGACGCCT GGCCCACCCA GGGCTCATCA 
CTGGGTTCCG GCGCGTCCAG CCAGGAGCAC CTGGGGCCCC CCTCCAAGGC GGGCACCCCC 
GCCTCCACGC TCACCAAGAG CGGCCCCGGG CGCTGGAAGA CCCCGGCCGC CTCCCCGCCC 
ATGCGGACCG ACCCGGCCCT GCCGCCACCG CCGCCCCCCG GGTCCTACGC CGGGGAGGCT 
GACGCGGACG CGGACTCGCC CCCGCCTCCC GCCGCCACCG CCGCCGGCCA GGCGCAGGCG 
GCCGCCGAGC GCAAGAAACG AGAGGAGCAG CAGCGCTGGT ACGAGAAGGA AAAAGCGCGC 
CTGGACGAGG AGCGCGAGCG CCGGCGCCGG GAGCAGGAGC GCCGGCTGGG CCAGCTGCGC 
GCGCCCGCGC CGCCCCCCGC GCCCGCCCGC GCGCCCGACA CGGTGATCCG CGAGCTGCAG 
CCGCAGCAGC AGCCGCGTAC CATCGAGCGC AGGGACCTGC AGTACATCAC GGTCAGCAGG 
GAGGAGCTGG CCTCCGGAGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA TGCGCGCGAG 
AAGTTGGAGA AGCAGCAGCA GCTGCACATC GTGGACCTGC TGAGCCGCGA GATCCAGGAG 
CTGCAGGGCA AGGCCGAGCG CTCGGCCGAG GAGAGCGACC GGCTGCGCAA GCTGCTGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGAGGAG 
GAGGAGGACG ATGACGTGGA CACCGTGCTG CTCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGTTGCAGGA TGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG ACACGGGAGG CGGAGGAGCG CGCGCGGCAG 
GAGGAGCAGC GGCGCCGGCA GGAGGACGAG CGGGTCAGGC GCGAGGCCGA AGACAAGAGG 
CGGCAGGAGG ACGGCTACTA CAGCCGCCTG GAGGCCGAGC GGCGCCGACA GCATGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC 
GGCTATGAGC CCGCCGGCCC GCCGCCACCG CCCCAGCGCA CCGCCTCCTA CCTGCAGACG 
CAGGCCTTCT CCCCGGACGC CCTCTACACC GCCAAGTTCG TGGCCTACAA TGAGGAGGAG 
GACGAGGACG AAGAGCCGGC AGGAGCAAAC TCTTACGTGG GACCTGCTGG GACAGTTTTT 
GGAGCCCATG ATGTTTATCG GGACCCGAGA GAGAGACCGC CTAAGAGCCA CGAAGCAGAT 
GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC CTGACGTTCA GGGAGCGCCA GCGCCTTTTC 
TCCCAAGGCC ACGATGTCTC CAATAAAGTC AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG 
AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997141.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852909.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

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CloneID	OBa238179
Clone ID Related Accession (Same CDS sequence)	XM_024997141.1
Accession Version	XM_024997141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA GTGAGGGAGC CTGTGGCCCT TCCCAAGGCC CGCCCCACAC AGCTGCTCAC 
CACCGGGGTG GGGGCCGCTC GGTCAATAAG GAATGTTCTC GCCTGCGTAG ATTAGCCAAC 
TGGGATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852909.1
CDS	71..5536
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997141.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997141.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA GTGAGGGAGC CTGTGGCCCT TCCCAAGGCC CGCCCCACAC AGCTGCTCAC 
CACCGGGGTG GGGGCCGCTC GGTCAATAAG GAATGTTCTC GCCTGCGTAG ATTAGCCAAC 
TGGGATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997142.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852910.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OBa238180
Clone ID Related Accession (Same CDS sequence)	XM_024997142.1
Accession Version	XM_024997142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAGTGAGG GAGCCTGTGG CCCTTCCCAA GGCCCGCCCC ACACAGCTGC TCACCACCGG 
GGTGGGGGCC GCTCGGTCAA TAAGGAATGT TCTCGCCTGC GTAGATTAGC CAACTGGGAT 
CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG TATGCCTCCG GAACAGCAGC CAAGATCACA 
TCTGTCTCCA CGGGGAACCT CTGCGCTGAG GAGCAGACGC CTCCGCCCCG CCCCGAAGCC 
TACCCCATCC CCACGCAGAC GTCCACCAGG GAGTACTTCA CCTTCCCGGC CTCCAAGTCC 
CAGGACCGCG TGGCGCCCCC GCAGAGCCAG TGGCTGGGTT TCGAGGAGAA GCCCCACGTG 
CTGGCAGACG GAAACCACTC CAGCGCTGCC GTCCAGCGTG TGACCCGCTC CCAGGAGGAG 
CTCCGCGAGG ACGCCTACCA CCCCGAGCGG CAGCGGCCGG AGGCAGCCGC GGATCGCAGG 
TCGGATGGCA GCGACGCCTG GCCCACCCAG GGCTCATCAC TGGGTTCCGG CGCGTCCAGC 
CAGGAGCACC TGGGGCCCCC CTCCAAGGCG GGCACCCCCG CCTCCACGCT CACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC TCCCCGCCCA TGCGGACCGA CCCGGCCCTG 
CCGCCACCGC CGCCCCCCGG GTCCTACGCC GGGGAGGCTG ACGCGGACGC GGACTCGCCC 
CCGCCTCCCG CCGCCACCGC CGCCGGCCAG GCGCAGGCGG CCGCCGAGCG CAAGAAACGA 
GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA AAAGCGCGCC TGGACGAGGA GCGCGAGCGC 
CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG 
CCCGCCCGCG CGCCCGACAC GGTGATCCGC GAGCTGCAGC CGCAGCAGCA GCCGCGTACC 
ATCGAGCGCA GGGACCTGCA GTACATCACG GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAT GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG 
CTGCACATCG TGGACCTGCT GAGCCGCGAG ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC 
TCGGCCGAGG AGAGCGACCG GCTGCGCAAG CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA TGACGTGGAC 
ACCGTGCTGC TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTATGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAT GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGA CACGGGAGGC GGAGGAGCGC GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG 
GAGGACGAGC GGGTCAGGCG CGAGGCCGAA GACAAGAGGC GGCAGGAGGA CGGCTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCCGACAG CATGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA CTCCCGCGCG GCTATGAGCC CGCCGGCCCG 
CCGCCACCGC CCCAGCGCAC CGCCTCCTAC CTGCAGACGC AGGCCTTCTC CCCGGACGCC 
CTCTACACCG CCAAGTTCGT GGCCTACAAT GAGGAGGAGG ACGAGGACGA AGAGCCGGCA 
GGAGCAAACT CTTACGTGGG ACCTGCTGGG ACAGTTTTTG GAGCCCATGA TGTTTATCGG 
GACCCGAGAG AGAGACCGCC TAAGAGCCAC GAAGCAGATG TCCCCGGGGG TCCCGGAGCC 
CCTGAAAACC TGACGTTCAG GGAGCGCCAG CGCCTTTTCT CCCAAGGCCA CGATGTCTCC 
AATAAAGTCA AGGCCTCTCG CAAGTTAACA GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852910.1
CDS	72..5531
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997142.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997142.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAGTGAGG GAGCCTGTGG CCCTTCCCAA GGCCCGCCCC ACACAGCTGC TCACCACCGG 
GGTGGGGGCC GCTCGGTCAA TAAGGAATGT TCTCGCCTGC GTAGATTAGC CAACTGGGAT 
CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG TATGCCTCCG GAACAGCAGC CAAGATCACA 
TCTGTCTCCA CGGGGAACCT CTGCGCTGAG GAGCAGACGC CTCCGCCCCG CCCCGAAGCC 
TACCCCATCC CCACGCAGAC GTCCACCAGG GAGTACTTCA CCTTCCCGGC CTCCAAGTCC 
CAGGACCGCG TGGCGCCCCC GCAGAGCCAG TGGCTGGGTT TCGAGGAGAA GCCCCACGTG 
CTGGCAGACG GAAACCACTC CAGCGCTGCC GTCCAGCGTG TGACCCGCTC CCAGGAGGAG 
CTCCGCGAGG ACGCCTACCA CCCCGAGCGG CAGCGGCCGG AGGCAGCCGC GGATCGCAGG 
TCGGATGGCA GCGACGCCTG GCCCACCCAG GGCTCATCAC TGGGTTCCGG CGCGTCCAGC 
CAGGAGCACC TGGGGCCCCC CTCCAAGGCG GGCACCCCCG CCTCCACGCT CACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC TCCCCGCCCA TGCGGACCGA CCCGGCCCTG 
CCGCCACCGC CGCCCCCCGG GTCCTACGCC GGGGAGGCTG ACGCGGACGC GGACTCGCCC 
CCGCCTCCCG CCGCCACCGC CGCCGGCCAG GCGCAGGCGG CCGCCGAGCG CAAGAAACGA 
GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA AAAGCGCGCC TGGACGAGGA GCGCGAGCGC 
CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG 
CCCGCCCGCG CGCCCGACAC GGTGATCCGC GAGCTGCAGC CGCAGCAGCA GCCGCGTACC 
ATCGAGCGCA GGGACCTGCA GTACATCACG GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAT GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG 
CTGCACATCG TGGACCTGCT GAGCCGCGAG ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC 
TCGGCCGAGG AGAGCGACCG GCTGCGCAAG CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA TGACGTGGAC 
ACCGTGCTGC TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTATGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAT GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGA CACGGGAGGC GGAGGAGCGC GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG 
GAGGACGAGC GGGTCAGGCG CGAGGCCGAA GACAAGAGGC GGCAGGAGGA CGGCTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCCGACAG CATGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA CTCCCGCGCG GCTATGAGCC CGCCGGCCCG 
CCGCCACCGC CCCAGCGCAC CGCCTCCTAC CTGCAGACGC AGGCCTTCTC CCCGGACGCC 
CTCTACACCG CCAAGTTCGT GGCCTACAAT GAGGAGGAGG ACGAGGACGA AGAGCCGGCA 
GGAGCAAACT CTTACGTGGG ACCTGCTGGG ACAGTTTTTG GAGCCCATGA TGTTTATCGG 
GACCCGAGAG AGAGACCGCC TAAGAGCCAC GAAGCAGATG TCCCCGGGGG TCCCGGAGCC 
CCTGAAAACC TGACGTTCAG GGAGCGCCAG CGCCTTTTCT CCCAAGGCCA CGATGTCTCC 
AATAAAGTCA AGGCCTCTCG CAAGTTAACA GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997143.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852911.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OBa238181
Clone ID Related Accession (Same CDS sequence)	XM_024997143.1
Accession Version	XM_024997143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGTTGCAGGA TGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
ACACGGGAGG CGGAGGAGCG CGCGCGGCAG GAGGAGCAGC GGCGCCGGCA GGAGGACGAG 
CGGGTCAGGC GCGAGGCCGA AGACAAGAGG CGGCAGGAGG ACGGCTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCCGACA GCATGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC GGCTATGAGC CCGCCGGCCC GCCGCCACCG 
CCCCAGCGCA CCGCCTCCTA CCTGCAGACG CAGGCCTTCT CCCCGGACGC CCTCTACACC 
GCCAAGTTCG TGGCCTACAA TGAGGAGGAG GACGAGGACG AAGAGCCGGC AGGAGCAAAC 
TCTTACGTGG GACCTGCTGG GACAGTTTTT GGAGCCCATG ATGTTTATCG GGACCCGAGA 
GAGAGACCGC CTAAGAGCCA CGAAGCAGAT GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC 
CTGACGTTCA GGGAGCGCCA GCGCCTTTTC TCCCAAGGCC ACGATGTCTC CAATAAAGTC 
AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852911.1
CDS	71..5521
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997143.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997143.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGTTGCAGGA TGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
ACACGGGAGG CGGAGGAGCG CGCGCGGCAG GAGGAGCAGC GGCGCCGGCA GGAGGACGAG 
CGGGTCAGGC GCGAGGCCGA AGACAAGAGG CGGCAGGAGG ACGGCTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCCGACA GCATGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC GGCTATGAGC CCGCCGGCCC GCCGCCACCG 
CCCCAGCGCA CCGCCTCCTA CCTGCAGACG CAGGCCTTCT CCCCGGACGC CCTCTACACC 
GCCAAGTTCG TGGCCTACAA TGAGGAGGAG GACGAGGACG AAGAGCCGGC AGGAGCAAAC 
TCTTACGTGG GACCTGCTGG GACAGTTTTT GGAGCCCATG ATGTTTATCG GGACCCGAGA 
GAGAGACCGC CTAAGAGCCA CGAAGCAGAT GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC 
CTGACGTTCA GGGAGCGCCA GCGCCTTTTC TCCCAAGGCC ACGATGTCTC CAATAAAGTC 
AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997144.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852912.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OBa238182
Clone ID Related Accession (Same CDS sequence)	XM_024997144.1
Accession Version	XM_024997144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGGATGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGACACGG 
GAGGCGGAGG AGCGCGCGCG GCAGGAGGAG CAGCGGCGCC GGCAGGAGGA CGAGCGGGTC 
AGGCGCGAGG CCGAAGACAA GAGGCGGCAG GAGGACGGCT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCC GACAGCATGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CGCCACTCCC GCGCGGCTAT GAGCCCGCCG GCCCGCCGCC ACCGCCCCAG 
CGCACCGCCT CCTACCTGCA GACGCAGGCC TTCTCCCCGG ACGCCCTCTA CACCGCCAAG 
TTCGTGGCCT ACAATGAGGA GGAGGACGAG GACGAAGAGC CGGCAGGAGC AAACTCTTAC 
GTGGGACCTG CTGGGACAGT TTTTGGAGCC CATGATGTTT ATCGGGACCC GAGAGAGAGA 
CCGCCTAAGA GCCACGAAGC AGATGTCCCC GGGGGTCCCG GAGCCCCTGA AAACCTGACG 
TTCAGGGAGC GCCAGCGCCT TTTCTCCCAA GGCCACGATG TCTCCAATAA AGTCAAGGCC 
TCTCGCAAGT TAACAGAGCT GGAGAACGAG CTGAACACGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852912.1
CDS	71..5515
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997144.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997144.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGGATGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGACACGG 
GAGGCGGAGG AGCGCGCGCG GCAGGAGGAG CAGCGGCGCC GGCAGGAGGA CGAGCGGGTC 
AGGCGCGAGG CCGAAGACAA GAGGCGGCAG GAGGACGGCT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCC GACAGCATGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CGCCACTCCC GCGCGGCTAT GAGCCCGCCG GCCCGCCGCC ACCGCCCCAG 
CGCACCGCCT CCTACCTGCA GACGCAGGCC TTCTCCCCGG ACGCCCTCTA CACCGCCAAG 
TTCGTGGCCT ACAATGAGGA GGAGGACGAG GACGAAGAGC CGGCAGGAGC AAACTCTTAC 
GTGGGACCTG CTGGGACAGT TTTTGGAGCC CATGATGTTT ATCGGGACCC GAGAGAGAGA 
CCGCCTAAGA GCCACGAAGC AGATGTCCCC GGGGGTCCCG GAGCCCCTGA AAACCTGACG 
TTCAGGGAGC GCCAGCGCCT TTTCTCCCAA GGCCACGATG TCTCCAATAA AGTCAAGGCC 
TCTCGCAAGT TAACAGAGCT GGAGAACGAG CTGAACACGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997148.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852916.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OBa238183
Clone ID Related Accession (Same CDS sequence)	XM_024997148.1
Accession Version	XM_024997148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA ATCAGCCTCC CAGTCCTGGC GGGAAGAGTG CGTATGCCTC CGGAACAGCA 
GCCAAGATCA CATCTGTCTC CACGGGGAAC CTCTGCGCTG AGGAGCAGAC GCCTCCGCCC 
CGCCCCGAAG CCTACCCCAT CCCCACGCAG ACGTCCACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAAGT CCCAGGACCG CGTGGCGCCC CCGCAGAGCC AGTGGCTGGG TTTCGAGGAG 
AAGCCCCACG TGCTGGCAGA CGGAAACCAC TCCAGCGCTG CCGTCCAGCG TGTGACCCGC 
TCCCAGGAGG AGCTCCGCGA GGACGCCTAC CACCCCGAGC GGCAGCGGCC GGAGGCAGCC 
GCGGATCGCA GGTCGGATGG CAGCGACGCC TGGCCCACCC AGGGCTCATC ACTGGGTTCC 
GGCGCGTCCA GCCAGGAGCA CCTGGGGCCC CCCTCCAAGG CGGGCACCCC CGCCTCCACG 
CTCACCAAGA GCGGCCCCGG GCGCTGGAAG ACCCCGGCCG CCTCCCCGCC CATGCGGACC 
GACCCGGCCC TGCCGCCACC GCCGCCCCCC GGGTCCTACG CCGGGGAGGC TGACGCGGAC 
GCGGACTCGC CCCCGCCTCC CGCCGCCACC GCCGCCGGCC AGGCGCAGGC GGCCGCCGAG 
CGCAAGAAAC GAGAGGAGCA GCAGCGCTGG TACGAGAAGG AAAAAGCGCG CCTGGACGAG 
GAGCGCGAGC GCCGGCGCCG GGAGCAGGAG CGCCGGCTGG GCCAGCTGCG CGCGCCCGCG 
CCGCCCCCCG CGCCCGCCCG CGCGCCCGAC ACGGTGATCC GCGAGCTGCA GCCGCAGCAG 
CAGCCGCGTA CCATCGAGCG CAGGGACCTG CAGTACATCA CGGTCAGCAG GGAGGAGCTG 
GCCTCCGGAG ACAGCCTGTC CCCCGACCCC TGGAAGCGCG ATGCGCGCGA GAAGTTGGAG 
AAGCAGCAGC AGCTGCACAT CGTGGACCTG CTGAGCCGCG AGATCCAGGA GCTGCAGGGC 
AAGGCCGAGC GCTCGGCCGA GGAGAGCGAC CGGCTGCGCA AGCTGCTGCT GGAGTGGCAG 
TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG AAGGACGAGG ACGACGAGGA GGAGGAGGAC 
GATGACGTGG ACACCGTGCT GCTCATGCAG CGCCTGGAGG CCGAGCGCAG AGCGCGGTTG 
CAGGATGAGG AGCGGCGGCG GCAGCAGCAG CTGGAGGAGA TGCGGACACG GGAGGCGGAG 
GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG 
GCCGAAGACA AGAGGCGGCA GGAGGACGGC TACTACAGCC GCCTGGAGGC CGAGCGGCGC 
CGACAGCATG AGGAGGCCGA GCGCCGGCTG CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC 
CCGCCACTCC CGCGCGGCTA TGAGCCCGCC GGCCCGCCGC CACCGCCCCA GCGCACCGCC 
TCCTACCTGC AGACGCAGGC CTTCTCCCCG GACGCCCTCT ACACCGCCAA GTTCGTGGCC 
TACAATGAGG AGGAGGACGA GGACGAAGAG CCGGCAGGAG CAAACTCTTA CGTGGGACCT 
GCTGGGACAG TTTTTGGAGC CCATGATGTT TATCGGGACC CGAGAGAGAG ACCGCCTAAG 
AGCCACGAAG CAGATGTCCC CGGGGGTCCC GGAGCCCCTG AAAACCTGAC GTTCAGGGAG 
CGCCAGCGCC TTTTCTCCCA AGGCCACGAT GTCTCCAATA AAGTCAAGGC CTCTCGCAAG 
TTAACAGAGC TGGAGAACGA GCTGAACACG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852916.1
CDS	70..5385
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997148.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997148.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA ATCAGCCTCC CAGTCCTGGC GGGAAGAGTG CGTATGCCTC CGGAACAGCA 
GCCAAGATCA CATCTGTCTC CACGGGGAAC CTCTGCGCTG AGGAGCAGAC GCCTCCGCCC 
CGCCCCGAAG CCTACCCCAT CCCCACGCAG ACGTCCACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAAGT CCCAGGACCG CGTGGCGCCC CCGCAGAGCC AGTGGCTGGG TTTCGAGGAG 
AAGCCCCACG TGCTGGCAGA CGGAAACCAC TCCAGCGCTG CCGTCCAGCG TGTGACCCGC 
TCCCAGGAGG AGCTCCGCGA GGACGCCTAC CACCCCGAGC GGCAGCGGCC GGAGGCAGCC 
GCGGATCGCA GGTCGGATGG CAGCGACGCC TGGCCCACCC AGGGCTCATC ACTGGGTTCC 
GGCGCGTCCA GCCAGGAGCA CCTGGGGCCC CCCTCCAAGG CGGGCACCCC CGCCTCCACG 
CTCACCAAGA GCGGCCCCGG GCGCTGGAAG ACCCCGGCCG CCTCCCCGCC CATGCGGACC 
GACCCGGCCC TGCCGCCACC GCCGCCCCCC GGGTCCTACG CCGGGGAGGC TGACGCGGAC 
GCGGACTCGC CCCCGCCTCC CGCCGCCACC GCCGCCGGCC AGGCGCAGGC GGCCGCCGAG 
CGCAAGAAAC GAGAGGAGCA GCAGCGCTGG TACGAGAAGG AAAAAGCGCG CCTGGACGAG 
GAGCGCGAGC GCCGGCGCCG GGAGCAGGAG CGCCGGCTGG GCCAGCTGCG CGCGCCCGCG 
CCGCCCCCCG CGCCCGCCCG CGCGCCCGAC ACGGTGATCC GCGAGCTGCA GCCGCAGCAG 
CAGCCGCGTA CCATCGAGCG CAGGGACCTG CAGTACATCA CGGTCAGCAG GGAGGAGCTG 
GCCTCCGGAG ACAGCCTGTC CCCCGACCCC TGGAAGCGCG ATGCGCGCGA GAAGTTGGAG 
AAGCAGCAGC AGCTGCACAT CGTGGACCTG CTGAGCCGCG AGATCCAGGA GCTGCAGGGC 
AAGGCCGAGC GCTCGGCCGA GGAGAGCGAC CGGCTGCGCA AGCTGCTGCT GGAGTGGCAG 
TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG AAGGACGAGG ACGACGAGGA GGAGGAGGAC 
GATGACGTGG ACACCGTGCT GCTCATGCAG CGCCTGGAGG CCGAGCGCAG AGCGCGGTTG 
CAGGATGAGG AGCGGCGGCG GCAGCAGCAG CTGGAGGAGA TGCGGACACG GGAGGCGGAG 
GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG 
GCCGAAGACA AGAGGCGGCA GGAGGACGGC TACTACAGCC GCCTGGAGGC CGAGCGGCGC 
CGACAGCATG AGGAGGCCGA GCGCCGGCTG CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC 
CCGCCACTCC CGCGCGGCTA TGAGCCCGCC GGCCCGCCGC CACCGCCCCA GCGCACCGCC 
TCCTACCTGC AGACGCAGGC CTTCTCCCCG GACGCCCTCT ACACCGCCAA GTTCGTGGCC 
TACAATGAGG AGGAGGACGA GGACGAAGAG CCGGCAGGAG CAAACTCTTA CGTGGGACCT 
GCTGGGACAG TTTTTGGAGC CCATGATGTT TATCGGGACC CGAGAGAGAG ACCGCCTAAG 
AGCCACGAAG CAGATGTCCC CGGGGGTCCC GGAGCCCCTG AAAACCTGAC GTTCAGGGAG 
CGCCAGCGCC TTTTCTCCCA AGGCCACGAT GTCTCCAATA AAGTCAAGGC CTCTCGCAAG 
TTAACAGAGC TGGAGAACGA GCTGAACACG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997149.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852917.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OBa238184
Clone ID Related Accession (Same CDS sequence)	XM_024997149.1
Accession Version	XM_024997149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA ATCAGCCTCC CAGTCCTGGC GGGAAGAGTG CGTATGCCTC CGGAACAGCA 
GCCAAGATCA CATCTGTCTC CACGGGGAAC CTCTGCGCTG AGGAGCAGAC GCCTCCGCCC 
CGCCCCGAAG CCTACCCCAT CCCCACGCAG ACGTCCACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAAGT CCCAGGACCG CGTGGCGCCC CCGCAGAGCC AGTGGCTGGG TTTCGAGGAG 
AAGCCCCACG TGCTGGCAGA CGGAAACCAC TCCAGCGCTG CCGTCCAGCG TGTGACCCGC 
TCCCAGGAGG AGCTCCGCGA GGACGCCTAC CACCCCGAGC GGCAGCGGCC GGAGGCAGCC 
GCGGATCGCA GGTCGGATGG CAGCGACGCC TGGCCCACCC AGGGCTCATC ACTGGGTTCC 
GGCGCGTCCA GCCAGGAGCA CCTGGGGCCC CCCTCCAAGG CGGGCACCCC CGCCTCCACG 
CTCACCAAGA GCGGCCCCGG GCGCTGGAAG ACCCCGGCCG CCTCCCCGCC CATGCGGACC 
GACCCGGCCC TGCCGCCACC GCCGCCCCCC GGGTCCTACG CCGGGGAGGC TGACGCGGAC 
GCGGACTCGC CCCCGCCTCC CGCCGCCACC GCCGCCGGCC AGGCGCAGGC GGCCGCCGAG 
CGCAAGAAAC GAGAGGAGCA GCAGCGCTGG TACGAGAAGG AAAAAGCGCG CCTGGACGAG 
GAGCGCGAGC GCCGGCGCCG GGAGCAGGAG CGCCGGCTGG GCCAGCTGCG CGCGCCCGCG 
CCGCCCCCCG CGCCCGCCCG CGCGCCCGAC ACGGTGATCC GCGAGCTGCA GCCGCAGCAG 
CAGCCGCGTA CCATCGAGCG CAGGGACCTG CAGTACATCA CGGTCAGCAG GGAGGAGCTG 
GCCTCCGGAG ACAGCCTGTC CCCCGACCCC TGGAAGCGCG ATGCGCGCGA GAAGTTGGAG 
AAGCAGCAGC AGCTGCACAT CGTGGACCTG CTGAGCCGCG AGATCCAGGA GCTGCAGGGC 
AAGGCCGAGC GCTCGGCCGA GGAGAGCGAC CGGCTGCGCA AGCTGCTGCT GGAGTGGCAG 
TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG AAGGACGAGG ACGACGAGGA GGAGGAGGAC 
GATGACGTGG ACACCGTGCT GCTCATGCAG CGCCTGGAGG CCGAGCGCAG AGCGCGGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA 
CTCCCGCGCG GCTATGAGCC CGCCGGCCCG CCGCCACCGC CCCAGCGCAC CGCCTCCTAC 
CTGCAGACGC AGGCCTTCTC CCCGGACGCC CTCTACACCG CCAAGTTCGT GGCCTACAAT 
GAGGAGGAGG ACGAGGACGA AGAGCCGGCA GGAGCAAACT CTTACGTGGG ACCTGCTGGG 
ACAGTTTTTG GAGCCCATGA TGTTTATCGG GACCCGAGAG AGAGACCGCC TAAGAGCCAC 
GAAGCAGATG TCCCCGGGGG TCCCGGAGCC CCTGAAAACC TGACGTTCAG GGAGCGCCAG 
CGCCTTTTCT CCCAAGGCCA CGATGTCTCC AATAAAGTCA AGGCCTCTCG CAAGTTAACA 
GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852917.1
CDS	70..5379
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997149.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997149.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA ATCAGCCTCC CAGTCCTGGC GGGAAGAGTG CGTATGCCTC CGGAACAGCA 
GCCAAGATCA CATCTGTCTC CACGGGGAAC CTCTGCGCTG AGGAGCAGAC GCCTCCGCCC 
CGCCCCGAAG CCTACCCCAT CCCCACGCAG ACGTCCACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAAGT CCCAGGACCG CGTGGCGCCC CCGCAGAGCC AGTGGCTGGG TTTCGAGGAG 
AAGCCCCACG TGCTGGCAGA CGGAAACCAC TCCAGCGCTG CCGTCCAGCG TGTGACCCGC 
TCCCAGGAGG AGCTCCGCGA GGACGCCTAC CACCCCGAGC GGCAGCGGCC GGAGGCAGCC 
GCGGATCGCA GGTCGGATGG CAGCGACGCC TGGCCCACCC AGGGCTCATC ACTGGGTTCC 
GGCGCGTCCA GCCAGGAGCA CCTGGGGCCC CCCTCCAAGG CGGGCACCCC CGCCTCCACG 
CTCACCAAGA GCGGCCCCGG GCGCTGGAAG ACCCCGGCCG CCTCCCCGCC CATGCGGACC 
GACCCGGCCC TGCCGCCACC GCCGCCCCCC GGGTCCTACG CCGGGGAGGC TGACGCGGAC 
GCGGACTCGC CCCCGCCTCC CGCCGCCACC GCCGCCGGCC AGGCGCAGGC GGCCGCCGAG 
CGCAAGAAAC GAGAGGAGCA GCAGCGCTGG TACGAGAAGG AAAAAGCGCG CCTGGACGAG 
GAGCGCGAGC GCCGGCGCCG GGAGCAGGAG CGCCGGCTGG GCCAGCTGCG CGCGCCCGCG 
CCGCCCCCCG CGCCCGCCCG CGCGCCCGAC ACGGTGATCC GCGAGCTGCA GCCGCAGCAG 
CAGCCGCGTA CCATCGAGCG CAGGGACCTG CAGTACATCA CGGTCAGCAG GGAGGAGCTG 
GCCTCCGGAG ACAGCCTGTC CCCCGACCCC TGGAAGCGCG ATGCGCGCGA GAAGTTGGAG 
AAGCAGCAGC AGCTGCACAT CGTGGACCTG CTGAGCCGCG AGATCCAGGA GCTGCAGGGC 
AAGGCCGAGC GCTCGGCCGA GGAGAGCGAC CGGCTGCGCA AGCTGCTGCT GGAGTGGCAG 
TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG AAGGACGAGG ACGACGAGGA GGAGGAGGAC 
GATGACGTGG ACACCGTGCT GCTCATGCAG CGCCTGGAGG CCGAGCGCAG AGCGCGGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA 
CTCCCGCGCG GCTATGAGCC CGCCGGCCCG CCGCCACCGC CCCAGCGCAC CGCCTCCTAC 
CTGCAGACGC AGGCCTTCTC CCCGGACGCC CTCTACACCG CCAAGTTCGT GGCCTACAAT 
GAGGAGGAGG ACGAGGACGA AGAGCCGGCA GGAGCAAACT CTTACGTGGG ACCTGCTGGG 
ACAGTTTTTG GAGCCCATGA TGTTTATCGG GACCCGAGAG AGAGACCGCC TAAGAGCCAC 
GAAGCAGATG TCCCCGGGGG TCCCGGAGCC CCTGAAAACC TGACGTTCAG GGAGCGCCAG 
CGCCTTTTCT CCCAAGGCCA CGATGTCTCC AATAAAGTCA AGGCCTCTCG CAAGTTAACA 
GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997150.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852918.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

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CloneID	OBa238185
Clone ID Related Accession (Same CDS sequence)	XM_024997150.1
Accession Version	XM_024997150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGCT TCCTGCCAGG 
CTGTTTTGGT GGCGTGCGAC TTCTCCATCC TTCAGGGTAA AACAGCAAGC TCGTCTTCTC 
TCCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852918.1
CDS	72..5000
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLPARLFWWRATSPSFRVKQQARLLSI

Target ORF information:

RefSeq Version	XM_024997150.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997150.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGCT TCCTGCCAGG 
CTGTTTTGGT GGCGTGCGAC TTCTCCATCC TTCAGGGTAA AACAGCAAGC TCGTCTTCTC 
TCCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997151.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852919.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

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CloneID	OBa238186
Clone ID Related Accession (Same CDS sequence)	XM_024997151.1
Accession Version	XM_024997151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGTTGCAGG ATGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GACACGGGAG 
GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG CGGCGCCGGC AGGAGGACGA GCGGGTCAGG 
CGCGAGGCCG AAGACAAGAG GCGGCAGGAG GACGGCTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCCGAC AGCATGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCGC CACTCCCGCG CGGCTATGAG CCCGCCGGCC CGCCGCCACC GCCCCAGCGC 
ACCGCCTCCT ACCTGCAGAC GCAGGCCTTC TCCCCGGACG CCCTCTACAC CGCCAAGTTC 
GTGGCCTACA ATGAGGAGGA GGACGAGGAC GAAGAGCCGG CAGGAGCAAA CTCTTACGTG 
GGACCTGCTG GGACAGTTTT TGGAGCCCAT GATGTTTATC GGGACCCGAG AGAGAGACCG 
CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG GGTCCCGGAG CCCCTGAAAA CCTGACGTTC 
AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC CACGATGTCT CCAATAAAGT CAAGGCCTCT 
CGCAAGTTAA CAGAGCTGGA GAACGAGCTG AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852919.1
CDS	68..4609
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVASEGACGPSQGPPHTAAHHRGGGRSVNKECSRLRRLANWDQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997151.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997151.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AGTGAGGGAG CCTGTGGCCC TTCCCAAGGC 
CCGCCCCACA CAGCTGCTCA CCACCGGGGT GGGGGCCGCT CGGTCAATAA GGAATGTTCT 
CGCCTGCGTA GATTAGCCAA CTGGGATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGTTGCAGG ATGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GACACGGGAG 
GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG CGGCGCCGGC AGGAGGACGA GCGGGTCAGG 
CGCGAGGCCG AAGACAAGAG GCGGCAGGAG GACGGCTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCCGAC AGCATGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCGC CACTCCCGCG CGGCTATGAG CCCGCCGGCC CGCCGCCACC GCCCCAGCGC 
ACCGCCTCCT ACCTGCAGAC GCAGGCCTTC TCCCCGGACG CCCTCTACAC CGCCAAGTTC 
GTGGCCTACA ATGAGGAGGA GGACGAGGAC GAAGAGCCGG CAGGAGCAAA CTCTTACGTG 
GGACCTGCTG GGACAGTTTT TGGAGCCCAT GATGTTTATC GGGACCCGAG AGAGAGACCG 
CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG GGTCCCGGAG CCCCTGAAAA CCTGACGTTC 
AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC CACGATGTCT CCAATAAAGT CAAGGCCTCT 
CGCAAGTTAA CAGAGCTGGA GAACGAGCTG AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808756.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807058.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

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CloneID	OBa06241
Clone ID Related Accession (Same CDS sequence)	XM_010808756.2
Accession Version	XM_010808756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGGGAGAA GGAACCACTC GGCCTGCTAC 
AGCTCTCACG CTTCCGAAGA CGGTTCTGAC TCCAGAGACA AGCCAAAGCT CTACCGCCTT 
CAGCTCAGCG TCACGGAGGT GGGGACCGAG AAGTTCGACG ACAGCTCCAT CCAGTTGTTT 
GGTTCAGGCA TTCAGCCCCA TCACTGTGAC CTCACGAATA TGGACGGGGT GGTCACGGTC 
ACGCCGAGAA GCATGGACGC TGAGACCTAC GTGGATGGGC AGCGCATCTC GGAGACCACG 
ATGCTGCAGA GCGGGATGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTCGTGGAC 
CCCAGCCAGG ACCACACGCT CCCCAAGAGA TCCGTGGACG GGGGCCTGAC TGTGAAGGGC 
CCCAGACATA AGTCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTAGGAGG AGACGTTCAC 
AGTGGAATGG CCTTACCAGC GAGCAAGAGC ACCGCCAGGC TGGACAGCGA CAGGACGCCG 
TCCACCTCCA GCACCGCCGA GCGGGGGATG GTGAAGCCGA TGGTCCGCGT GGAGCAGCAG 
CTGGACTACC GCCGGCAGGA CAGCAGAACT CAGGATGCTC CCGGGCCTGA CCTGATTCTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCCT TTTTATCTGC CATTATAAAT 
TACACTAATA GCTCTACAGT CCATTTTAAG TTGTCGCCCA CTTACGTGTT ATACATGGCC 
TGTCGGTATG TATTGTCCAG CCAGTACAGA CCCGACGTCA GCCCCGCGGA ACGCACGCAC 
AAAGTCATGG CCATCGTCAA CAAGATGGTG AGCATGATGG AAGGGGTGAT CCAGAAACAG 
AAGAACATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAACG CGTCCGAGCT GCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGGATC ACACTGGATG CACAGGACGT CCTGGCACAC 
TTGGTGCAGA TGGCCTTTAA GTACCTGGTT CACTGTCTTC AGTCAGAGCT GAATAATTAC 
ATGCCCGCTT TTCTGGACGA CCCCGAAGAG GACAGTCTGC AGAGACCAAA AATAGACGAC 
GTGCTGCACA CGCTCACGGG CGCCATGTCC CTGCTGCGTC GCTGCCGGGT CAATGCCGCG 
CTGACCATCC AGCTCTTCTC CCAACTCTTC CACTTCATCA ACATGTGGCT GTTCAACCGG 
CTGGTGACCG ACCCCGAGTC GGGGCTCTGC TCCCACTACT GGGGTGCCAT CCTCCGCCAG 
CAGCTCGGGC ACGTGGAGGC CTGGGCCGAG AAGCAGGGCC TGGAGCTGGC GGCTGACTGC 
CACCTGAGCC GGATAGTGCA GGCAACCACT TTGCTTACGA TGGATAAGTA CGCACCTGAT 
GACATTCCAA ATATAAACAG CACCTGCTTC AAGTTAAATT CACTGCAGCT CCAAGCCTTA 
CTGCAGAATT ACCACTGTGC ACCCGATGAG CCTTTTGTCC CTGCGGACCT GATCGCGAAC 
GTGGTGGCCG TGGCCGAGAA CACAGCAGAC GAGCTGGCAC GCAGCGACGG GCGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCTTTCCTCC TGCCTGAGGA CGGCTACTCC 
TGTGACGTCG TCAGGAATGT CCCCAGCGGC CTGCAGGAGT TCCTGGACCC TCTCTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCACCCA CGCTCTCCAG GCACCTGGAC GATATATTTT 
GAAGGTGCAG ATTACGAGAG TCACCTTTTG CGGGAGAACA CAGAACTGGC ACAGCCTCTG 
CGTAAAGAAC CTGAAATCAT CACTGTGACC CTCAAAAAGC AGAACGGAAT GGGCCTTAGC 
ATTGTTGCTG CAAAGGGCGC TGGTCAAGAC AAACTTGGGA TTTACGTCAA GTCCGTTGTG 
AAAGGAGGCG CTGCGGACGT GGACGGGCGG CTCGCCGCGG GTGACCAGCT GCTCAGCGTG 
GACGGACGGA GTCTGGTGGG ACTCTCTCAA GAAAGGGCAG CAGAACTGAT GACAAGGACA 
AGCTCCGTCG TGACGCTTGA AGTAGCAAAG CAAGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCTTCCCC TATGATGCAG AGAATTTCAG AGCGCCGTGG GTCAGGTAAA 
CCCCGACCAA AAAGCGAAGG CTTTGAGCTG TACAACAACT CGGCTCAGAA CGGGTCCCCG 
GAGAGCCCGC AGACGCCCTG GGCCGAGTAC AGCGAGCCCA AGAAGCTGCC GGGCGATGAC 
CGGCTGACGA AGCACAGGGC AGACCACCGC TCCAGCCCCA ACGTCGCAAA TCAGCCTCCC 
AGTCCTGGCG GGAAGAGTGC GTATGCCTCC GGAACAGCAG CCAAGATCAC ATCTGTCTCC 
ACGGGGAACC TCTGCGCTGA GGAGCAGACG CCTCCGCCCC GCCCCGAAGC CTACCCCATC 
CCCACGCAGA CGTCCACCAG GGAGTACTTC ACCTTCCCGG CCTCCAAGTC CCAGGACCGC 
GTGGCGCCCC CGCAGAGCCA GTGGCTGGGT TTCGAGGAGA AGCCCCACGT GCTGGCAGAC 
GGAAACCACT CCAGCGCTGC CGTCCAGCGT GTGACCCGCT CCCAGGAGGA GCTCCGCGAG 
GACGCCTACC ACCCCGAGCG GCAGCGGCCG GAGGCAGCCG CGGATCGCAG GTCGGATGGC 
AGCGACGCCT GGCCCACCCA GGGCTCATCA CTGGGTTCCG GCGCGTCCAG CCAGGAGCAC 
CTGGGGCCCC CCTCCAAGGC GGGCACCCCC GCCTCCACGC TCACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CCCCGGCCGC CTCCCCGCCC ATGCGGACCG ACCCGGCCCT GCCGCCACCG 
CCGCCCCCCG GGTCCTACGC CGGGGAGGCT GACGCGGACG CGGACTCGCC CCCGCCTCCC 
GCCGCCACCG CCGCCGGCCA GGCGCAGGCG GCCGCCGAGC GCAAGAAACG AGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA AAAAGCGCGC CTGGACGAGG AGCGCGAGCG CCGGCGCCGG 
GAGCAGGAGC GCCGGCTGGG CCAGCTGCGC GCGCCCGCGC CGCCCCCCGC GCCCGCCCGC 
GCGCCCGACA CGGTGATCCG CGAGCTGCAG CCGCAGCAGC AGCCGCGTAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC GGTCAGCAGG GAGGAGCTGG CCTCCGGAGA CAGCCTGTCC 
CCCGACCCCT GGAAGCGCGA TGCGCGCGAG AAGTTGGAGA AGCAGCAGCA GCTGCACATC 
GTGGACCTGC TGAGCCGCGA GATCCAGGAG CTGCAGGGCA AGGCCGAGCG CTCGGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTGCTGCTG GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG 
TCCAAGCAGA AGGACGAGGA CGACGAGGAG GAGGAGGACG ATGACGTGGA CACCGTGCTG 
CTCATGCAGC GCCTGGAGGC CGAGCGCAGA GCGCGGGATG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGAC ACGGGAGGCG GAGGAGCGCG CGCGGCAGGA GGAGCAGCGG 
CGCCGGCAGG AGGACGAGCG GGTCAGGCGC GAGGCCGAAG ACAAGAGGCG GCAGGAGGAC 
GGCTACTACA GCCGCCTGGA GGCCGAGCGG CGCCGACAGC ATGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCGCCAC TCCCGCGCGG CTATGAGCCC 
GCCGGCCCGC CGCCACCGCC CCAGCGCACC GCCTCCTACC TGCAGACGCA GGCCTTCTCC 
CCGGACGCCC TCTACACCGC CAAGTTCGTG GCCTACAATG AGGAGGAGGA CGAGGACGAA 
GAGCCGGCAG GAGCAAACTC TTACGTGGGA CCTGCTGGGA CAGTTTTTGG AGCCCATGAT 
GTTTATCGGG ACCCGAGAGA GAGACCGCCT AAGAGCCACG AAGCAGATGT CCCCGGGGGT 
CCCGGAGCCC CTGAAAACCT GACGTTCAGG GAGCGCCAGC GCCTTTTCTC CCAAGGCCAC 
GATGTCTCCA ATAAAGTCAA GGCCTCTCGC AAGTTAACAG AGCTGGAGAA CGAGCTGAAC 
ACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807058.1
CDS	55..5283
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_010808756.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808756.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGGGAGAA GGAACCACTC GGCCTGCTAC 
AGCTCTCACG CTTCCGAAGA CGGTTCTGAC TCCAGAGACA AGCCAAAGCT CTACCGCCTT 
CAGCTCAGCG TCACGGAGGT GGGGACCGAG AAGTTCGACG ACAGCTCCAT CCAGTTGTTT 
GGTTCAGGCA TTCAGCCCCA TCACTGTGAC CTCACGAATA TGGACGGGGT GGTCACGGTC 
ACGCCGAGAA GCATGGACGC TGAGACCTAC GTGGATGGGC AGCGCATCTC GGAGACCACG 
ATGCTGCAGA GCGGGATGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTCGTGGAC 
CCCAGCCAGG ACCACACGCT CCCCAAGAGA TCCGTGGACG GGGGCCTGAC TGTGAAGGGC 
CCCAGACATA AGTCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTAGGAGG AGACGTTCAC 
AGTGGAATGG CCTTACCAGC GAGCAAGAGC ACCGCCAGGC TGGACAGCGA CAGGACGCCG 
TCCACCTCCA GCACCGCCGA GCGGGGGATG GTGAAGCCGA TGGTCCGCGT GGAGCAGCAG 
CTGGACTACC GCCGGCAGGA CAGCAGAACT CAGGATGCTC CCGGGCCTGA CCTGATTCTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCCT TTTTATCTGC CATTATAAAT 
TACACTAATA GCTCTACAGT CCATTTTAAG TTGTCGCCCA CTTACGTGTT ATACATGGCC 
TGTCGGTATG TATTGTCCAG CCAGTACAGA CCCGACGTCA GCCCCGCGGA ACGCACGCAC 
AAAGTCATGG CCATCGTCAA CAAGATGGTG AGCATGATGG AAGGGGTGAT CCAGAAACAG 
AAGAACATTG CAGGGGCCCT TGCCTTCTGG ATGGCCAACG CGTCCGAGCT GCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGGATC ACACTGGATG CACAGGACGT CCTGGCACAC 
TTGGTGCAGA TGGCCTTTAA GTACCTGGTT CACTGTCTTC AGTCAGAGCT GAATAATTAC 
ATGCCCGCTT TTCTGGACGA CCCCGAAGAG GACAGTCTGC AGAGACCAAA AATAGACGAC 
GTGCTGCACA CGCTCACGGG CGCCATGTCC CTGCTGCGTC GCTGCCGGGT CAATGCCGCG 
CTGACCATCC AGCTCTTCTC CCAACTCTTC CACTTCATCA ACATGTGGCT GTTCAACCGG 
CTGGTGACCG ACCCCGAGTC GGGGCTCTGC TCCCACTACT GGGGTGCCAT CCTCCGCCAG 
CAGCTCGGGC ACGTGGAGGC CTGGGCCGAG AAGCAGGGCC TGGAGCTGGC GGCTGACTGC 
CACCTGAGCC GGATAGTGCA GGCAACCACT TTGCTTACGA TGGATAAGTA CGCACCTGAT 
GACATTCCAA ATATAAACAG CACCTGCTTC AAGTTAAATT CACTGCAGCT CCAAGCCTTA 
CTGCAGAATT ACCACTGTGC ACCCGATGAG CCTTTTGTCC CTGCGGACCT GATCGCGAAC 
GTGGTGGCCG TGGCCGAGAA CACAGCAGAC GAGCTGGCAC GCAGCGACGG GCGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCTTTCCTCC TGCCTGAGGA CGGCTACTCC 
TGTGACGTCG TCAGGAATGT CCCCAGCGGC CTGCAGGAGT TCCTGGACCC TCTCTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCACCCA CGCTCTCCAG GCACCTGGAC GATATATTTT 
GAAGGTGCAG ATTACGAGAG TCACCTTTTG CGGGAGAACA CAGAACTGGC ACAGCCTCTG 
CGTAAAGAAC CTGAAATCAT CACTGTGACC CTCAAAAAGC AGAACGGAAT GGGCCTTAGC 
ATTGTTGCTG CAAAGGGCGC TGGTCAAGAC AAACTTGGGA TTTACGTCAA GTCCGTTGTG 
AAAGGAGGCG CTGCGGACGT GGACGGGCGG CTCGCCGCGG GTGACCAGCT GCTCAGCGTG 
GACGGACGGA GTCTGGTGGG ACTCTCTCAA GAAAGGGCAG CAGAACTGAT GACAAGGACA 
AGCTCCGTCG TGACGCTTGA AGTAGCAAAG CAAGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCTTCCCC TATGATGCAG AGAATTTCAG AGCGCCGTGG GTCAGGTAAA 
CCCCGACCAA AAAGCGAAGG CTTTGAGCTG TACAACAACT CGGCTCAGAA CGGGTCCCCG 
GAGAGCCCGC AGACGCCCTG GGCCGAGTAC AGCGAGCCCA AGAAGCTGCC GGGCGATGAC 
CGGCTGACGA AGCACAGGGC AGACCACCGC TCCAGCCCCA ACGTCGCAAA TCAGCCTCCC 
AGTCCTGGCG GGAAGAGTGC GTATGCCTCC GGAACAGCAG CCAAGATCAC ATCTGTCTCC 
ACGGGGAACC TCTGCGCTGA GGAGCAGACG CCTCCGCCCC GCCCCGAAGC CTACCCCATC 
CCCACGCAGA CGTCCACCAG GGAGTACTTC ACCTTCCCGG CCTCCAAGTC CCAGGACCGC 
GTGGCGCCCC CGCAGAGCCA GTGGCTGGGT TTCGAGGAGA AGCCCCACGT GCTGGCAGAC 
GGAAACCACT CCAGCGCTGC CGTCCAGCGT GTGACCCGCT CCCAGGAGGA GCTCCGCGAG 
GACGCCTACC ACCCCGAGCG GCAGCGGCCG GAGGCAGCCG CGGATCGCAG GTCGGATGGC 
AGCGACGCCT GGCCCACCCA GGGCTCATCA CTGGGTTCCG GCGCGTCCAG CCAGGAGCAC 
CTGGGGCCCC CCTCCAAGGC GGGCACCCCC GCCTCCACGC TCACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CCCCGGCCGC CTCCCCGCCC ATGCGGACCG ACCCGGCCCT GCCGCCACCG 
CCGCCCCCCG GGTCCTACGC CGGGGAGGCT GACGCGGACG CGGACTCGCC CCCGCCTCCC 
GCCGCCACCG CCGCCGGCCA GGCGCAGGCG GCCGCCGAGC GCAAGAAACG AGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA AAAAGCGCGC CTGGACGAGG AGCGCGAGCG CCGGCGCCGG 
GAGCAGGAGC GCCGGCTGGG CCAGCTGCGC GCGCCCGCGC CGCCCCCCGC GCCCGCCCGC 
GCGCCCGACA CGGTGATCCG CGAGCTGCAG CCGCAGCAGC AGCCGCGTAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC GGTCAGCAGG GAGGAGCTGG CCTCCGGAGA CAGCCTGTCC 
CCCGACCCCT GGAAGCGCGA TGCGCGCGAG AAGTTGGAGA AGCAGCAGCA GCTGCACATC 
GTGGACCTGC TGAGCCGCGA GATCCAGGAG CTGCAGGGCA AGGCCGAGCG CTCGGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTGCTGCTG GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG 
TCCAAGCAGA AGGACGAGGA CGACGAGGAG GAGGAGGACG ATGACGTGGA CACCGTGCTG 
CTCATGCAGC GCCTGGAGGC CGAGCGCAGA GCGCGGGATG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGAC ACGGGAGGCG GAGGAGCGCG CGCGGCAGGA GGAGCAGCGG 
CGCCGGCAGG AGGACGAGCG GGTCAGGCGC GAGGCCGAAG ACAAGAGGCG GCAGGAGGAC 
GGCTACTACA GCCGCCTGGA GGCCGAGCGG CGCCGACAGC ATGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCGCCAC TCCCGCGCGG CTATGAGCCC 
GCCGGCCCGC CGCCACCGCC CCAGCGCACC GCCTCCTACC TGCAGACGCA GGCCTTCTCC 
CCGGACGCCC TCTACACCGC CAAGTTCGTG GCCTACAATG AGGAGGAGGA CGAGGACGAA 
GAGCCGGCAG GAGCAAACTC TTACGTGGGA CCTGCTGGGA CAGTTTTTGG AGCCCATGAT 
GTTTATCGGG ACCCGAGAGA GAGACCGCCT AAGAGCCACG AAGCAGATGT CCCCGGGGGT 
CCCGGAGCCC CTGAAAACCT GACGTTCAGG GAGCGCCAGC GCCTTTTCTC CCAAGGCCAC 
GATGTCTCCA ATAAAGTCAA GGCCTCTCGC AAGTTAACAG AGCTGGAGAA CGAGCTGAAC 
ACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808749.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807051.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa46103
Clone ID Related Accession (Same CDS sequence)	XM_010808749.2
Accession Version	XM_010808749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGATGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGACAC GGGAGGCGGA GGAGCGCGCG 
CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG GACGAGCGGG TCAGGCGCGA GGCCGAAGAC 
AAGAGGCGGC AGGAGGACGG CTACTACAGC CGCCTGGAGG CCGAGCGGCG CCGACAGCAT 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCGCCACTC 
CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG CCACCGCCCC AGCGCACCGC CTCCTACCTG 
CAGACGCAGG CCTTCTCCCC GGACGCCCTC TACACCGCCA AGTTCGTGGC CTACAATGAG 
GAGGAGGACG AGGACGAAGA GCCGGCAGGA GCAAACTCTT ACGTGGGACC TGCTGGGACA 
GTTTTTGGAG CCCATGATGT TTATCGGGAC CCGAGAGAGA GACCGCCTAA GAGCCACGAA 
GCAGATGTCC CCGGGGGTCC CGGAGCCCCT GAAAACCTGA CGTTCAGGGA GCGCCAGCGC 
CTTTTCTCCC AAGGCCACGA TGTCTCCAAT AAAGTCAAGG CCTCTCGCAA GTTAACAGAG 
CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807051.1
CDS	56..5302
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_010808749.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808749.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGATGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGACAC GGGAGGCGGA GGAGCGCGCG 
CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG GACGAGCGGG TCAGGCGCGA GGCCGAAGAC 
AAGAGGCGGC AGGAGGACGG CTACTACAGC CGCCTGGAGG CCGAGCGGCG CCGACAGCAT 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCGCCACTC 
CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG CCACCGCCCC AGCGCACCGC CTCCTACCTG 
CAGACGCAGG CCTTCTCCCC GGACGCCCTC TACACCGCCA AGTTCGTGGC CTACAATGAG 
GAGGAGGACG AGGACGAAGA GCCGGCAGGA GCAAACTCTT ACGTGGGACC TGCTGGGACA 
GTTTTTGGAG CCCATGATGT TTATCGGGAC CCGAGAGAGA GACCGCCTAA GAGCCACGAA 
GCAGATGTCC CCGGGGGTCC CGGAGCCCCT GAAAACCTGA CGTTCAGGGA GCGCCAGCGC 
CTTTTCTCCC AAGGCCACGA TGTCTCCAAT AAAGTCAAGG CCTCTCGCAA GTTAACAGAG 
CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808750.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807052.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

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CloneID	OBa46104
Clone ID Related Accession (Same CDS sequence)	XM_010808750.2
Accession Version	XM_010808750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGTTGCAGGA TGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
ACACGGGAGG CGGAGGAGCG CGCGCGGCAG GAGGAGCAGC GGCGCCGGCA GGAGGACGAG 
CGGGTCAGGC GCGAGGCCGA AGACAAGAGG CGGCAGGAGG ACGGCTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCCGACA GCATGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC GGCTATGAGC CCGCCGGCCC GCCGCCACCG 
CCCCAGCGCA CCGCCTCCTA CCTGCAGACG CAGGCCTTCT CCCCGGACGC CCTCTACACC 
GCCAAGTTCG TGGCCTACAA TGAGGAGGAG GACGAGGACG AAGAGCCGGC AGGAGCAAAC 
TCTTACGTGG GACCTGCTGG GACAGTTTTT GGAGCCCATG ATGTTTATCG GGACCCGAGA 
GAGAGACCGC CTAAGAGCCA CGAAGCAGAT GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC 
CTGACGTTCA GGGAGCGCCA GCGCCTTTTC TCCCAAGGCC ACGATGTCTC CAATAAAGTC 
AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807052.1
CDS	56..5266
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_010808750.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808750.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGTTGCAGGA TGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
ACACGGGAGG CGGAGGAGCG CGCGCGGCAG GAGGAGCAGC GGCGCCGGCA GGAGGACGAG 
CGGGTCAGGC GCGAGGCCGA AGACAAGAGG CGGCAGGAGG ACGGCTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCCGACA GCATGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC GGCTATGAGC CCGCCGGCCC GCCGCCACCG 
CCCCAGCGCA CCGCCTCCTA CCTGCAGACG CAGGCCTTCT CCCCGGACGC CCTCTACACC 
GCCAAGTTCG TGGCCTACAA TGAGGAGGAG GACGAGGACG AAGAGCCGGC AGGAGCAAAC 
TCTTACGTGG GACCTGCTGG GACAGTTTTT GGAGCCCATG ATGTTTATCG GGACCCGAGA 
GAGAGACCGC CTAAGAGCCA CGAAGCAGAT GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC 
CTGACGTTCA GGGAGCGCCA GCGCCTTTTC TCCCAAGGCC ACGATGTCTC CAATAAAGTC 
AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808751.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807053.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

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CloneID	OBa46105
Clone ID Related Accession (Same CDS sequence)	XM_010808751.2
Accession Version	XM_010808751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGGATGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGACACGG 
GAGGCGGAGG AGCGCGCGCG GCAGGAGGAG CAGCGGCGCC GGCAGGAGGA CGAGCGGGTC 
AGGCGCGAGG CCGAAGACAA GAGGCGGCAG GAGGACGGCT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCC GACAGCATGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CGCCACTCCC GCGCGGCTAT GAGCCCGCCG GCCCGCCGCC ACCGCCCCAG 
CGCACCGCCT CCTACCTGCA GACGCAGGCC TTCTCCCCGG ACGCCCTCTA CACCGCCAAG 
TTCGTGGCCT ACAATGAGGA GGAGGACGAG GACGAAGAGC CGGCAGGAGC AAACTCTTAC 
GTGGGACCTG CTGGGACAGT TTTTGGAGCC CATGATGTTT ATCGGGACCC GAGAGAGAGA 
CCGCCTAAGA GCCACGAAGC AGATGTCCCC GGGGGTCCCG GAGCCCCTGA AAACCTGACG 
TTCAGGGAGC GCCAGCGCCT TTTCTCCCAA GGCCACGATG TCTCCAATAA AGTCAAGGCC 
TCTCGCAAGT TAACAGAGCT GGAGAACGAG CTGAACACGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807053.1
CDS	56..5260
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_010808751.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808751.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGGATGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGACACGG 
GAGGCGGAGG AGCGCGCGCG GCAGGAGGAG CAGCGGCGCC GGCAGGAGGA CGAGCGGGTC 
AGGCGCGAGG CCGAAGACAA GAGGCGGCAG GAGGACGGCT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCC GACAGCATGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CGCCACTCCC GCGCGGCTAT GAGCCCGCCG GCCCGCCGCC ACCGCCCCAG 
CGCACCGCCT CCTACCTGCA GACGCAGGCC TTCTCCCCGG ACGCCCTCTA CACCGCCAAG 
TTCGTGGCCT ACAATGAGGA GGAGGACGAG GACGAAGAGC CGGCAGGAGC AAACTCTTAC 
GTGGGACCTG CTGGGACAGT TTTTGGAGCC CATGATGTTT ATCGGGACCC GAGAGAGAGA 
CCGCCTAAGA GCCACGAAGC AGATGTCCCC GGGGGTCCCG GAGCCCCTGA AAACCTGACG 
TTCAGGGAGC GCCAGCGCCT TTTCTCCCAA GGCCACGATG TCTCCAATAA AGTCAAGGCC 
TCTCGCAAGT TAACAGAGCT GGAGAACGAG CTGAACACGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808755.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807057.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

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CloneID	OBa46109
Clone ID Related Accession (Same CDS sequence)	XM_010808755.2
Accession Version	XM_010808755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GGGAGAAGGA ACCACTCGGC CTGCTACAGC 
TCTCACGCTT CCGAAGACGG TTCTGACTCC AGAGACAAGC CAAAGCTCTA CCGCCTTCAG 
CTCAGCGTCA CGGAGGTGGG GACCGAGAAG TTCGACGACA GCTCCATCCA GTTGTTTGGT 
TCAGGCATTC AGCCCCATCA CTGTGACCTC ACGAATATGG ACGGGGTGGT CACGGTCACG 
CCGAGAAGCA TGGACGCTGA GACCTACGTG GATGGGCAGC GCATCTCGGA GACCACGATG 
CTGCAGAGCG GGATGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT CGTGGACCCC 
AGCCAGGACC ACACGCTCCC CAAGAGATCC GTGGACGGGG GCCTGACTGT GAAGGGCCCC 
AGACATAAGT CTGGAATTGT TCAGGAGACA ACTTTTGATT TAGGAGGAGA CGTTCACAGT 
GGAATGGCCT TACCAGCGAG CAAGAGCACC GCCAGGCTGG ACAGCGACAG GACGCCGTCC 
ACCTCCAGCA CCGCCGAGCG GGGGATGGTG AAGCCGATGG TCCGCGTGGA GCAGCAGCTG 
GACTACCGCC GGCAGGACAG CAGAACTCAG GATGCTCCCG GGCCTGACCT GATTCTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCCTTTT TATCTGCCAT TATAAATTAC 
ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCGCCCACTT ACGTGTTATA CATGGCCTGT 
CGGTATGTAT TGTCCAGCCA GTACAGACCC GACGTCAGCC CCGCGGAACG CACGCACAAA 
GTCATGGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GGGTGATCCA GAAACAGAAG 
AACATTGCAG GGGCCCTTGC CTTCTGGATG GCCAACGCGT CCGAGCTGCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGGATCACA CTGGATGCAC AGGACGTCCT GGCACACTTG 
GTGCAGATGG CCTTTAAGTA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA TAATTACATG 
CCCGCTTTTC TGGACGACCC CGAAGAGGAC AGTCTGCAGA GACCAAAAAT AGACGACGTG 
CTGCACACGC TCACGGGCGC CATGTCCCTG CTGCGTCGCT GCCGGGTCAA TGCCGCGCTG 
ACCATCCAGC TCTTCTCCCA ACTCTTCCAC TTCATCAACA TGTGGCTGTT CAACCGGCTG 
GTGACCGACC CCGAGTCGGG GCTCTGCTCC CACTACTGGG GTGCCATCCT CCGCCAGCAG 
CTCGGGCACG TGGAGGCCTG GGCCGAGAAG CAGGGCCTGG AGCTGGCGGC TGACTGCCAC 
CTGAGCCGGA TAGTGCAGGC AACCACTTTG CTTACGATGG ATAAGTACGC ACCTGATGAC 
ATTCCAAATA TAAACAGCAC CTGCTTCAAG TTAAATTCAC TGCAGCTCCA AGCCTTACTG 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTGTCCCTG CGGACCTGAT CGCGAACGTG 
GTGGCCGTGG CCGAGAACAC AGCAGACGAG CTGGCACGCA GCGACGGGCG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCT TTCCTCCTGC CTGAGGACGG CTACTCCTGT 
GACGTCGTCA GGAATGTCCC CAGCGGCCTG CAGGAGTTCC TGGACCCTCT CTGCCAGAGA 
GGGTTTTGCA GGTTAATTCC TCACCCACGC TCTCCAGGCA CCTGGACGAT ATATTTTGAA 
GGTGCAGATT ACGAGAGTCA CCTTTTGCGG GAGAACACAG AACTGGCACA GCCTCTGCGT 
AAAGAACCTG AAATCATCAC TGTGACCCTC AAAAAGCAGA ACGGAATGGG CCTTAGCATT 
GTTGCTGCAA AGGGCGCTGG TCAAGACAAA CTTGGGATTT ACGTCAAGTC CGTTGTGAAA 
GGAGGCGCTG CGGACGTGGA CGGGCGGCTC GCCGCGGGTG ACCAGCTGCT CAGCGTGGAC 
GGACGGAGTC TGGTGGGACT CTCTCAAGAA AGGGCAGCAG AACTGATGAC AAGGACAAGC 
TCCGTCGTGA CGCTTGAAGT AGCAAAGCAA GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CTTCCCCTAT GATGCAGAGA ATTTCAGAGC GCCGTGGGTC AGGTAAACCC 
CGACCAAAAA GCGAAGGCTT TGAGCTGTAC AACAACTCGG CTCAGAACGG GTCCCCGGAG 
AGCCCGCAGA CGCCCTGGGC CGAGTACAGC GAGCCCAAGA AGCTGCCGGG CGATGACCGG 
CTGACGAAGC ACAGGGCAGA CCACCGCTCC AGCCCCAACG TCGCAAATCA GCCTCCCAGT 
CCTGGCGGGA AGAGTGCGTA TGCCTCCGGA ACAGCAGCCA AGATCACATC TGTCTCCACG 
GGGAACCTCT GCGCTGAGGA GCAGACGCCT CCGCCCCGCC CCGAAGCCTA CCCCATCCCC 
ACGCAGACGT CCACCAGGGA GTACTTCACC TTCCCGGCCT CCAAGTCCCA GGACCGCGTG 
GCGCCCCCGC AGAGCCAGTG GCTGGGTTTC GAGGAGAAGC CCCACGTGCT GGCAGACGGA 
AACCACTCCA GCGCTGCCGT CCAGCGTGTG ACCCGCTCCC AGGAGGAGCT CCGCGAGGAC 
GCCTACCACC CCGAGCGGCA GCGGCCGGAG GCAGCCGCGG ATCGCAGGTC GGATGGCAGC 
GACGCCTGGC CCACCCAGGG CTCATCACTG GGTTCCGGCG CGTCCAGCCA GGAGCACCTG 
GGGCCCCCCT CCAAGGCGGG CACCCCCGCC TCCACGCTCA CCAAGAGCGG CCCCGGGCGC 
TGGAAGACCC CGGCCGCCTC CCCGCCCATG CGGACCGACC CGGCCCTGCC GCCACCGCCG 
CCCCCCGGGT CCTACGCCGG GGAGGCTGAC GCGGACGCGG ACTCGCCCCC GCCTCCCGCC 
GCCACCGCCG CCGGCCAGGC GCAGGCGGCC GCCGAGCGCA AGAAACGAGA GGAGCAGCAG 
CGCTGGTACG AGAAGGAAAA AGCGCGCCTG GACGAGGAGC GCGAGCGCCG GCGCCGGGAG 
CAGGAGCGCC GGCTGGGCCA GCTGCGCGCG CCCGCGCCGC CCCCCGCGCC CGCCCGCGCG 
CCCGACACGG TGATCCGCGA GCTGCAGCCG CAGCAGCAGC CGCGTACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACGGT CAGCAGGGAG GAGCTGGCCT CCGGAGACAG CCTGTCCCCC 
GACCCCTGGA AGCGCGATGC GCGCGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTG 
GACCTGCTGA GCCGCGAGAT CCAGGAGCTG CAGGGCAAGG CCGAGCGCTC GGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT GCTGCTGGAG TGGCAGTTCC AGAAGCGGCT GCAGGAGTCC 
AAGCAGAAGG ACGAGGACGA CGAGGAGGAG GAGGACGATG ACGTGGACAC CGTGCTGCTC 
ATGCAGCGCC TGGAGGCCGA GCGCAGAGCG CGGTTGCAGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGAG GCGGCAGGAG 
GACGGCTACT ACAGCCGCCT GGAGGCCGAG CGGCGCCGAC AGCATGAGGA GGCCGAGCGC 
CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG GGCCGCCCGC CACTCCCGCG CGGCTATGAG 
CCCGCCGGCC CGCCGCCACC GCCCCAGCGC ACCGCCTCCT ACCTGCAGAC GCAGGCCTTC 
TCCCCGGACG CCCTCTACAC CGCCAAGTTC GTGGCCTACA ATGAGGAGGA GGACGAGGAC 
GAAGAGCCGG CAGGAGCAAA CTCTTACGTG GGACCTGCTG GGACAGTTTT TGGAGCCCAT 
GATGTTTATC GGGACCCGAG AGAGAGACCG CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG 
GGTCCCGGAG CCCCTGAAAA CCTGACGTTC AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC 
CACGATGTCT CCAATAAAGT CAAGGCCTCT CGCAAGTTAA CAGAGCTGGA GAACGAGCTG 
AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807057.1
CDS	54..5285
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_010808755.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808755.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GGGAGAAGGA ACCACTCGGC CTGCTACAGC 
TCTCACGCTT CCGAAGACGG TTCTGACTCC AGAGACAAGC CAAAGCTCTA CCGCCTTCAG 
CTCAGCGTCA CGGAGGTGGG GACCGAGAAG TTCGACGACA GCTCCATCCA GTTGTTTGGT 
TCAGGCATTC AGCCCCATCA CTGTGACCTC ACGAATATGG ACGGGGTGGT CACGGTCACG 
CCGAGAAGCA TGGACGCTGA GACCTACGTG GATGGGCAGC GCATCTCGGA GACCACGATG 
CTGCAGAGCG GGATGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT CGTGGACCCC 
AGCCAGGACC ACACGCTCCC CAAGAGATCC GTGGACGGGG GCCTGACTGT GAAGGGCCCC 
AGACATAAGT CTGGAATTGT TCAGGAGACA ACTTTTGATT TAGGAGGAGA CGTTCACAGT 
GGAATGGCCT TACCAGCGAG CAAGAGCACC GCCAGGCTGG ACAGCGACAG GACGCCGTCC 
ACCTCCAGCA CCGCCGAGCG GGGGATGGTG AAGCCGATGG TCCGCGTGGA GCAGCAGCTG 
GACTACCGCC GGCAGGACAG CAGAACTCAG GATGCTCCCG GGCCTGACCT GATTCTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCCTTTT TATCTGCCAT TATAAATTAC 
ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCGCCCACTT ACGTGTTATA CATGGCCTGT 
CGGTATGTAT TGTCCAGCCA GTACAGACCC GACGTCAGCC CCGCGGAACG CACGCACAAA 
GTCATGGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GGGTGATCCA GAAACAGAAG 
AACATTGCAG GGGCCCTTGC CTTCTGGATG GCCAACGCGT CCGAGCTGCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGGATCACA CTGGATGCAC AGGACGTCCT GGCACACTTG 
GTGCAGATGG CCTTTAAGTA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA TAATTACATG 
CCCGCTTTTC TGGACGACCC CGAAGAGGAC AGTCTGCAGA GACCAAAAAT AGACGACGTG 
CTGCACACGC TCACGGGCGC CATGTCCCTG CTGCGTCGCT GCCGGGTCAA TGCCGCGCTG 
ACCATCCAGC TCTTCTCCCA ACTCTTCCAC TTCATCAACA TGTGGCTGTT CAACCGGCTG 
GTGACCGACC CCGAGTCGGG GCTCTGCTCC CACTACTGGG GTGCCATCCT CCGCCAGCAG 
CTCGGGCACG TGGAGGCCTG GGCCGAGAAG CAGGGCCTGG AGCTGGCGGC TGACTGCCAC 
CTGAGCCGGA TAGTGCAGGC AACCACTTTG CTTACGATGG ATAAGTACGC ACCTGATGAC 
ATTCCAAATA TAAACAGCAC CTGCTTCAAG TTAAATTCAC TGCAGCTCCA AGCCTTACTG 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTGTCCCTG CGGACCTGAT CGCGAACGTG 
GTGGCCGTGG CCGAGAACAC AGCAGACGAG CTGGCACGCA GCGACGGGCG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCT TTCCTCCTGC CTGAGGACGG CTACTCCTGT 
GACGTCGTCA GGAATGTCCC CAGCGGCCTG CAGGAGTTCC TGGACCCTCT CTGCCAGAGA 
GGGTTTTGCA GGTTAATTCC TCACCCACGC TCTCCAGGCA CCTGGACGAT ATATTTTGAA 
GGTGCAGATT ACGAGAGTCA CCTTTTGCGG GAGAACACAG AACTGGCACA GCCTCTGCGT 
AAAGAACCTG AAATCATCAC TGTGACCCTC AAAAAGCAGA ACGGAATGGG CCTTAGCATT 
GTTGCTGCAA AGGGCGCTGG TCAAGACAAA CTTGGGATTT ACGTCAAGTC CGTTGTGAAA 
GGAGGCGCTG CGGACGTGGA CGGGCGGCTC GCCGCGGGTG ACCAGCTGCT CAGCGTGGAC 
GGACGGAGTC TGGTGGGACT CTCTCAAGAA AGGGCAGCAG AACTGATGAC AAGGACAAGC 
TCCGTCGTGA CGCTTGAAGT AGCAAAGCAA GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CTTCCCCTAT GATGCAGAGA ATTTCAGAGC GCCGTGGGTC AGGTAAACCC 
CGACCAAAAA GCGAAGGCTT TGAGCTGTAC AACAACTCGG CTCAGAACGG GTCCCCGGAG 
AGCCCGCAGA CGCCCTGGGC CGAGTACAGC GAGCCCAAGA AGCTGCCGGG CGATGACCGG 
CTGACGAAGC ACAGGGCAGA CCACCGCTCC AGCCCCAACG TCGCAAATCA GCCTCCCAGT 
CCTGGCGGGA AGAGTGCGTA TGCCTCCGGA ACAGCAGCCA AGATCACATC TGTCTCCACG 
GGGAACCTCT GCGCTGAGGA GCAGACGCCT CCGCCCCGCC CCGAAGCCTA CCCCATCCCC 
ACGCAGACGT CCACCAGGGA GTACTTCACC TTCCCGGCCT CCAAGTCCCA GGACCGCGTG 
GCGCCCCCGC AGAGCCAGTG GCTGGGTTTC GAGGAGAAGC CCCACGTGCT GGCAGACGGA 
AACCACTCCA GCGCTGCCGT CCAGCGTGTG ACCCGCTCCC AGGAGGAGCT CCGCGAGGAC 
GCCTACCACC CCGAGCGGCA GCGGCCGGAG GCAGCCGCGG ATCGCAGGTC GGATGGCAGC 
GACGCCTGGC CCACCCAGGG CTCATCACTG GGTTCCGGCG CGTCCAGCCA GGAGCACCTG 
GGGCCCCCCT CCAAGGCGGG CACCCCCGCC TCCACGCTCA CCAAGAGCGG CCCCGGGCGC 
TGGAAGACCC CGGCCGCCTC CCCGCCCATG CGGACCGACC CGGCCCTGCC GCCACCGCCG 
CCCCCCGGGT CCTACGCCGG GGAGGCTGAC GCGGACGCGG ACTCGCCCCC GCCTCCCGCC 
GCCACCGCCG CCGGCCAGGC GCAGGCGGCC GCCGAGCGCA AGAAACGAGA GGAGCAGCAG 
CGCTGGTACG AGAAGGAAAA AGCGCGCCTG GACGAGGAGC GCGAGCGCCG GCGCCGGGAG 
CAGGAGCGCC GGCTGGGCCA GCTGCGCGCG CCCGCGCCGC CCCCCGCGCC CGCCCGCGCG 
CCCGACACGG TGATCCGCGA GCTGCAGCCG CAGCAGCAGC CGCGTACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACGGT CAGCAGGGAG GAGCTGGCCT CCGGAGACAG CCTGTCCCCC 
GACCCCTGGA AGCGCGATGC GCGCGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTG 
GACCTGCTGA GCCGCGAGAT CCAGGAGCTG CAGGGCAAGG CCGAGCGCTC GGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT GCTGCTGGAG TGGCAGTTCC AGAAGCGGCT GCAGGAGTCC 
AAGCAGAAGG ACGAGGACGA CGAGGAGGAG GAGGACGATG ACGTGGACAC CGTGCTGCTC 
ATGCAGCGCC TGGAGGCCGA GCGCAGAGCG CGGTTGCAGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGAG GCGGCAGGAG 
GACGGCTACT ACAGCCGCCT GGAGGCCGAG CGGCGCCGAC AGCATGAGGA GGCCGAGCGC 
CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG GGCCGCCCGC CACTCCCGCG CGGCTATGAG 
CCCGCCGGCC CGCCGCCACC GCCCCAGCGC ACCGCCTCCT ACCTGCAGAC GCAGGCCTTC 
TCCCCGGACG CCCTCTACAC CGCCAAGTTC GTGGCCTACA ATGAGGAGGA GGACGAGGAC 
GAAGAGCCGG CAGGAGCAAA CTCTTACGTG GGACCTGCTG GGACAGTTTT TGGAGCCCAT 
GATGTTTATC GGGACCCGAG AGAGAGACCG CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG 
GGTCCCGGAG CCCCTGAAAA CCTGACGTTC AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC 
CACGATGTCT CCAATAAAGT CAAGGCCTCT CGCAAGTTAA CAGAGCTGGA GAACGAGCTG 
AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808757.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807059.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

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CloneID	OBa46110
Clone ID Related Accession (Same CDS sequence)	XM_010808757.2
Accession Version	XM_010808757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808757.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTC ATCCAGAACT TTAAGAGAAC TCTCTCAAAG 
AAGGAGAAGA AGGAAAAAAA GAAGAGAGAA AAAGAGGCAG TGAGACAGGC GTCTGAGAAA 
GATGACAGAC CTTTCTCTGG GGATGACACT GAGAATTCTC GCCTGGCCGC TGAGGTCTAC 
AAAGACATGC CTGAGACCAG CTTTACGCGA ACCATCTCCA ACCCTGAGGT GGTCATGAAG 
CGGCGGCGGC AGCAGAAGCT GGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCAGACTCGG GTGGAACATT GAGAATTTAC GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACGATCC TGCTGTCCAC TACAGACCCT GCGGACTTTG CTGTGGCCGA AGCCTTGGAG 
AAGTACGGCC TGGAGAAGGA GAACCCCAGG GACTACTGCA TCGCCCGTGT CATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAAAAAGGT GCTAAAGAAG TTATTCTTGA TGATGACGAA 
TGTCCTTTAC AGATCTTCAG GGAATGGCCG AGTGACAGAG GGATTTTAGT CTTTCAGTTG 
AAGAGGAGGC CACCAGACCA CATCCCAAAG AAAAGTAAGA AGCACACGGA CGGGAAGCCC 
CTGCAGGGGA AGGAGAGAGC CGATGGGTCG GCCTACGGCT CCGCCCTGCC CCCCGAGAAG 
CTGCCCTACC TCGTGGAGCT GAGCCCGGAC GGTTCTGACT CCAGAGACAA GCCAAAGCTC 
TACCGCCTTC AGCTCAGCGT CACGGAGGTG GGGACCGAGA AGTTCGACGA CAGCTCCATC 
CAGTTGTTTG GTTCAGGCAT TCAGCCCCAT CACTGTGACC TCACGAATAT GGACGGGGTG 
GTCACGGTCA CGCCGAGAAG CATGGACGCT GAGACCTACG TGGATGGGCA GCGCATCTCG 
GAGACCACGA TGCTGCAGAG CGGGATGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTCGTGGACC CCAGCCAGGA CCACACGCTC CCCAAGAGAT CCGTGGACGG GGGCCTGACT 
GTGAAGGGCC CCAGACATAA GTCTGGAATT GTTCAGGAGA CAACTTTTGA TTTAGGAGGA 
GACGTTCACA GTGGAATGGC CTTACCAGCG AGCAAGAGCA CCGCCAGGCT GGACAGCGAC 
AGGACGCCGT CCACCTCCAG CACCGCCGAG CGGGGGATGG TGAAGCCGAT GGTCCGCGTG 
GAGCAGCAGC TGGACTACCG CCGGCAGGAC AGCAGAACTC AGGATGCTCC CGGGCCTGAC 
CTGATTCTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCCTT TTTATCTGCC 
ATTATAAATT ACACTAATAG CTCTACAGTC CATTTTAAGT TGTCGCCCAC TTACGTGTTA 
TACATGGCCT GTCGGTATGT ATTGTCCAGC CAGTACAGAC CCGACGTCAG CCCCGCGGAA 
CGCACGCACA AAGTCATGGC CATCGTCAAC AAGATGGTGA GCATGATGGA AGGGGTGATC 
CAGAAACAGA AGAACATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAACGC GTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGGATCA CACTGGATGC ACAGGACGTC 
CTGGCACACT TGGTGCAGAT GGCCTTTAAG TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AATAATTACA TGCCCGCTTT TCTGGACGAC CCCGAAGAGG ACAGTCTGCA GAGACCAAAA 
ATAGACGACG TGCTGCACAC GCTCACGGGC GCCATGTCCC TGCTGCGTCG CTGCCGGGTC 
AATGCCGCGC TGACCATCCA GCTCTTCTCC CAACTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACCGGC TGGTGACCGA CCCCGAGTCG GGGCTCTGCT CCCACTACTG GGGTGCCATC 
CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG 
GCTGACTGCC ACCTGAGCCG GATAGTGCAG GCAACCACTT TGCTTACGAT GGATAAGTAC 
GCACCTGATG ACATTCCAAA TATAAACAGC ACCTGCTTCA AGTTAAATTC ACTGCAGCTC 
CAAGCCTTAC TGCAGAATTA CCACTGTGCA CCCGATGAGC CTTTTGTCCC TGCGGACCTG 
ATCGCGAACG TGGTGGCCGT GGCCGAGAAC ACAGCAGACG AGCTGGCACG CAGCGACGGG 
CGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC 
GGCTACTCCT GTGACGTCGT CAGGAATGTC CCCAGCGGCC TGCAGGAGTT CCTGGACCCT 
CTCTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCACCCAC GCTCTCCAGG CACCTGGACG 
ATATATTTTG AAGGTGCAGA TTACGAGAGT CACCTTTTGC GGGAGAACAC AGAACTGGCA 
CAGCCTCTGC GTAAAGAACC TGAAATCATC ACTGTGACCC TCAAAAAGCA GAACGGAATG 
GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT GGTCAAGACA AACTTGGGAT TTACGTCAAG 
TCCGTTGTGA AAGGAGGCGC TGCGGACGTG GACGGGCGGC TCGCCGCGGG TGACCAGCTG 
CTCAGCGTGG ACGGACGGAG TCTGGTGGGA CTCTCTCAAG AAAGGGCAGC AGAACTGATG 
ACAAGGACAA GCTCCGTCGT GACGCTTGAA GTAGCAAAGC AAGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCTTCCCCT ATGATGCAGA GAATTTCAGA GCGCCGTGGG 
TCAGGTAAAC CCCGACCAAA AAGCGAAGGC TTTGAGCTGT ACAACAACTC GGCTCAGAAC 
GGGTCCCCGG AGAGCCCGCA GACGCCCTGG GCCGAGTACA GCGAGCCCAA GAAGCTGCCG 
GGCGATGACC GGCTGACGAA GCACAGGGCA GACCACCGCT CCAGCCCCAA CGTCGCAAAT 
CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG TATGCCTCCG GAACAGCAGC CAAGATCACA 
TCTGTCTCCA CGGGGAACCT CTGCGCTGAG GAGCAGACGC CTCCGCCCCG CCCCGAAGCC 
TACCCCATCC CCACGCAGAC GTCCACCAGG GAGTACTTCA CCTTCCCGGC CTCCAAGTCC 
CAGGACCGCG TGGCGCCCCC GCAGAGCCAG TGGCTGGGTT TCGAGGAGAA GCCCCACGTG 
CTGGCAGACG GAAACCACTC CAGCGCTGCC GTCCAGCGTG TGACCCGCTC CCAGGAGGAG 
CTCCGCGAGG ACGCCTACCA CCCCGAGCGG CAGCGGCCGG AGGCAGCCGC GGATCGCAGG 
TCGGATGGCA GCGACGCCTG GCCCACCCAG GGCTCATCAC TGGGTTCCGG CGCGTCCAGC 
CAGGAGCACC TGGGGCCCCC CTCCAAGGCG GGCACCCCCG CCTCCACGCT CACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC TCCCCGCCCA TGCGGACCGA CCCGGCCCTG 
CCGCCACCGC CGCCCCCCGG GTCCTACGCC GGGGAGGCTG ACGCGGACGC GGACTCGCCC 
CCGCCTCCCG CCGCCACCGC CGCCGGCCAG GCGCAGGCGG CCGCCGAGCG CAAGAAACGA 
GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA AAAGCGCGCC TGGACGAGGA GCGCGAGCGC 
CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG 
CCCGCCCGCG CGCCCGACAC GGTGATCCGC GAGCTGCAGC CGCAGCAGCA GCCGCGTACC 
ATCGAGCGCA GGGACCTGCA GTACATCACG GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAT GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG 
CTGCACATCG TGGACCTGCT GAGCCGCGAG ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC 
TCGGCCGAGG AGAGCGACCG GCTGCGCAAG CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA TGACGTGGAC 
ACCGTGCTGC TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGAG CAAACTCTTA CGTGGGACCT 
GCTGGGACAG TTTTTGGAGC CCATGATGTT TATCGGGACC CGAGAGAGAG ACCGCCTAAG 
AGCCACGAAG CAGATGTCCC CGGGGGTCCC GGAGCCCCTG AAAACCTGAC GTTCAGGGAG 
CGCCAGCGCC TTTTCTCCCA AGGCCACGAT GTCTCCAATA AAGTCAAGGC CTCTCGCAAG 
TTAACAGAGC TGGAGAACGA GCTGAACACG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807059.1
CDS	53..5068
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_010808757.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808757.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTC ATCCAGAACT TTAAGAGAAC TCTCTCAAAG 
AAGGAGAAGA AGGAAAAAAA GAAGAGAGAA AAAGAGGCAG TGAGACAGGC GTCTGAGAAA 
GATGACAGAC CTTTCTCTGG GGATGACACT GAGAATTCTC GCCTGGCCGC TGAGGTCTAC 
AAAGACATGC CTGAGACCAG CTTTACGCGA ACCATCTCCA ACCCTGAGGT GGTCATGAAG 
CGGCGGCGGC AGCAGAAGCT GGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCAGACTCGG GTGGAACATT GAGAATTTAC GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACGATCC TGCTGTCCAC TACAGACCCT GCGGACTTTG CTGTGGCCGA AGCCTTGGAG 
AAGTACGGCC TGGAGAAGGA GAACCCCAGG GACTACTGCA TCGCCCGTGT CATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAAAAAGGT GCTAAAGAAG TTATTCTTGA TGATGACGAA 
TGTCCTTTAC AGATCTTCAG GGAATGGCCG AGTGACAGAG GGATTTTAGT CTTTCAGTTG 
AAGAGGAGGC CACCAGACCA CATCCCAAAG AAAAGTAAGA AGCACACGGA CGGGAAGCCC 
CTGCAGGGGA AGGAGAGAGC CGATGGGTCG GCCTACGGCT CCGCCCTGCC CCCCGAGAAG 
CTGCCCTACC TCGTGGAGCT GAGCCCGGAC GGTTCTGACT CCAGAGACAA GCCAAAGCTC 
TACCGCCTTC AGCTCAGCGT CACGGAGGTG GGGACCGAGA AGTTCGACGA CAGCTCCATC 
CAGTTGTTTG GTTCAGGCAT TCAGCCCCAT CACTGTGACC TCACGAATAT GGACGGGGTG 
GTCACGGTCA CGCCGAGAAG CATGGACGCT GAGACCTACG TGGATGGGCA GCGCATCTCG 
GAGACCACGA TGCTGCAGAG CGGGATGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTCGTGGACC CCAGCCAGGA CCACACGCTC CCCAAGAGAT CCGTGGACGG GGGCCTGACT 
GTGAAGGGCC CCAGACATAA GTCTGGAATT GTTCAGGAGA CAACTTTTGA TTTAGGAGGA 
GACGTTCACA GTGGAATGGC CTTACCAGCG AGCAAGAGCA CCGCCAGGCT GGACAGCGAC 
AGGACGCCGT CCACCTCCAG CACCGCCGAG CGGGGGATGG TGAAGCCGAT GGTCCGCGTG 
GAGCAGCAGC TGGACTACCG CCGGCAGGAC AGCAGAACTC AGGATGCTCC CGGGCCTGAC 
CTGATTCTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCCTT TTTATCTGCC 
ATTATAAATT ACACTAATAG CTCTACAGTC CATTTTAAGT TGTCGCCCAC TTACGTGTTA 
TACATGGCCT GTCGGTATGT ATTGTCCAGC CAGTACAGAC CCGACGTCAG CCCCGCGGAA 
CGCACGCACA AAGTCATGGC CATCGTCAAC AAGATGGTGA GCATGATGGA AGGGGTGATC 
CAGAAACAGA AGAACATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAACGC GTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGGATCA CACTGGATGC ACAGGACGTC 
CTGGCACACT TGGTGCAGAT GGCCTTTAAG TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AATAATTACA TGCCCGCTTT TCTGGACGAC CCCGAAGAGG ACAGTCTGCA GAGACCAAAA 
ATAGACGACG TGCTGCACAC GCTCACGGGC GCCATGTCCC TGCTGCGTCG CTGCCGGGTC 
AATGCCGCGC TGACCATCCA GCTCTTCTCC CAACTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACCGGC TGGTGACCGA CCCCGAGTCG GGGCTCTGCT CCCACTACTG GGGTGCCATC 
CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG 
GCTGACTGCC ACCTGAGCCG GATAGTGCAG GCAACCACTT TGCTTACGAT GGATAAGTAC 
GCACCTGATG ACATTCCAAA TATAAACAGC ACCTGCTTCA AGTTAAATTC ACTGCAGCTC 
CAAGCCTTAC TGCAGAATTA CCACTGTGCA CCCGATGAGC CTTTTGTCCC TGCGGACCTG 
ATCGCGAACG TGGTGGCCGT GGCCGAGAAC ACAGCAGACG AGCTGGCACG CAGCGACGGG 
CGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC 
GGCTACTCCT GTGACGTCGT CAGGAATGTC CCCAGCGGCC TGCAGGAGTT CCTGGACCCT 
CTCTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCACCCAC GCTCTCCAGG CACCTGGACG 
ATATATTTTG AAGGTGCAGA TTACGAGAGT CACCTTTTGC GGGAGAACAC AGAACTGGCA 
CAGCCTCTGC GTAAAGAACC TGAAATCATC ACTGTGACCC TCAAAAAGCA GAACGGAATG 
GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT GGTCAAGACA AACTTGGGAT TTACGTCAAG 
TCCGTTGTGA AAGGAGGCGC TGCGGACGTG GACGGGCGGC TCGCCGCGGG TGACCAGCTG 
CTCAGCGTGG ACGGACGGAG TCTGGTGGGA CTCTCTCAAG AAAGGGCAGC AGAACTGATG 
ACAAGGACAA GCTCCGTCGT GACGCTTGAA GTAGCAAAGC AAGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCTTCCCCT ATGATGCAGA GAATTTCAGA GCGCCGTGGG 
TCAGGTAAAC CCCGACCAAA AAGCGAAGGC TTTGAGCTGT ACAACAACTC GGCTCAGAAC 
GGGTCCCCGG AGAGCCCGCA GACGCCCTGG GCCGAGTACA GCGAGCCCAA GAAGCTGCCG 
GGCGATGACC GGCTGACGAA GCACAGGGCA GACCACCGCT CCAGCCCCAA CGTCGCAAAT 
CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG TATGCCTCCG GAACAGCAGC CAAGATCACA 
TCTGTCTCCA CGGGGAACCT CTGCGCTGAG GAGCAGACGC CTCCGCCCCG CCCCGAAGCC 
TACCCCATCC CCACGCAGAC GTCCACCAGG GAGTACTTCA CCTTCCCGGC CTCCAAGTCC 
CAGGACCGCG TGGCGCCCCC GCAGAGCCAG TGGCTGGGTT TCGAGGAGAA GCCCCACGTG 
CTGGCAGACG GAAACCACTC CAGCGCTGCC GTCCAGCGTG TGACCCGCTC CCAGGAGGAG 
CTCCGCGAGG ACGCCTACCA CCCCGAGCGG CAGCGGCCGG AGGCAGCCGC GGATCGCAGG 
TCGGATGGCA GCGACGCCTG GCCCACCCAG GGCTCATCAC TGGGTTCCGG CGCGTCCAGC 
CAGGAGCACC TGGGGCCCCC CTCCAAGGCG GGCACCCCCG CCTCCACGCT CACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC TCCCCGCCCA TGCGGACCGA CCCGGCCCTG 
CCGCCACCGC CGCCCCCCGG GTCCTACGCC GGGGAGGCTG ACGCGGACGC GGACTCGCCC 
CCGCCTCCCG CCGCCACCGC CGCCGGCCAG GCGCAGGCGG CCGCCGAGCG CAAGAAACGA 
GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA AAAGCGCGCC TGGACGAGGA GCGCGAGCGC 
CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG 
CCCGCCCGCG CGCCCGACAC GGTGATCCGC GAGCTGCAGC CGCAGCAGCA GCCGCGTACC 
ATCGAGCGCA GGGACCTGCA GTACATCACG GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAT GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG 
CTGCACATCG TGGACCTGCT GAGCCGCGAG ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC 
TCGGCCGAGG AGAGCGACCG GCTGCGCAAG CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA TGACGTGGAC 
ACCGTGCTGC TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGAG CAAACTCTTA CGTGGGACCT 
GCTGGGACAG TTTTTGGAGC CCATGATGTT TATCGGGACC CGAGAGAGAG ACCGCCTAAG 
AGCCACGAAG CAGATGTCCC CGGGGGTCCC GGAGCCCCTG AAAACCTGAC GTTCAGGGAG 
CGCCAGCGCC TTTTCTCCCA AGGCCACGAT GTCTCCAATA AAGTCAAGGC CTCTCGCAAG 
TTAACAGAGC TGGAGAACGA GCTGAACACG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_010808758.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807060.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.

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CloneID	OBa46111
Clone ID Related Accession (Same CDS sequence)	XM_010808758.2
Accession Version	XM_010808758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GGGAGAAGGA ACCACTCGGC CTGCTACAGC 
TCTCACGCTT CCGAAGACGG TTCTGACTCC AGAGACAAGC CAAAGCTCTA CCGCCTTCAG 
CTCAGCGTCA CGGAGGTGGG GACCGAGAAG TTCGACGACA GCTCCATCCA GTTGTTTGGT 
TCAGGCATTC AGCCCCATCA CTGTGACCTC ACGAATATGG ACGGGGTGGT CACGGTCACG 
CCGAGAAGCA TGGACGCTGA GACCTACGTG GATGGGCAGC GCATCTCGGA GACCACGATG 
CTGCAGAGCG GGATGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT CGTGGACCCC 
AGCCAGGACC ACACGCTCCC CAAGAGATCC GTGGACGGGG GCCTGACTGT GAAGGGCCCC 
AGACATAAGT CTGGAATTGT TCAGGAGACA ACTTTTGATT TAGGAGGAGA CGTTCACAGT 
GGAATGGCCT TACCAGCGAG CAAGAGCACC GCCAGGCTGG ACAGCGACAG GACGCCGTCC 
ACCTCCAGCA CCGCCGAGCG GGGGATGGTG AAGCCGATGG TCCGCGTGGA GCAGCAGCTG 
GACTACCGCC GGCAGGACAG CAGAACTCAG GATGCTCCCG GGCCTGACCT GATTCTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCCTTTT TATCTGCCAT TATAAATTAC 
ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCGCCCACTT ACGTGTTATA CATGGCCTGT 
CGGTATGTAT TGTCCAGCCA GTACAGACCC GACGTCAGCC CCGCGGAACG CACGCACAAA 
GTCATGGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GGGTGATCCA GAAACAGAAG 
AACATTGCAG GGGCCCTTGC CTTCTGGATG GCCAACGCGT CCGAGCTGCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGGATCACA CTGGATGCAC AGGACGTCCT GGCACACTTG 
GTGCAGATGG CCTTTAAGTA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA TAATTACATG 
CCCGCTTTTC TGGACGACCC CGAAGAGGAC AGTCTGCAGA GACCAAAAAT AGACGACGTG 
CTGCACACGC TCACGGGCGC CATGTCCCTG CTGCGTCGCT GCCGGGTCAA TGCCGCGCTG 
ACCATCCAGC TCTTCTCCCA ACTCTTCCAC TTCATCAACA TGTGGCTGTT CAACCGGCTG 
GTGACCGACC CCGAGTCGGG GCTCTGCTCC CACTACTGGG GTGCCATCCT CCGCCAGCAG 
CTCGGGCACG TGGAGGCCTG GGCCGAGAAG CAGGGCCTGG AGCTGGCGGC TGACTGCCAC 
CTGAGCCGGA TAGTGCAGGC AACCACTTTG CTTACGATGG ATAAGTACGC ACCTGATGAC 
ATTCCAAATA TAAACAGCAC CTGCTTCAAG TTAAATTCAC TGCAGCTCCA AGCCTTACTG 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTGTCCCTG CGGACCTGAT CGCGAACGTG 
GTGGCCGTGG CCGAGAACAC AGCAGACGAG CTGGCACGCA GCGACGGGCG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCT TTCCTCCTGC CTGAGGACGG CTACTCCTGT 
GACGTCGTCA GGAATGTCCC CAGCGGCCTG CAGGAGTTCC TGGACCCTCT CTGCCAGAGA 
GGGTTTTGCA GGTTAATTCC TCACCCACGC TCTCCAGGCA CCTGGACGAT ATATTTTGAA 
GGTGCAGATT ACGAGAGTCA CCTTTTGCGG GAGAACACAG AACTGGCACA GCCTCTGCGT 
AAAGAACCTG AAATCATCAC TGTGACCCTC AAAAAGCAGA ACGGAATGGG CCTTAGCATT 
GTTGCTGCAA AGGGCGCTGG TCAAGACAAA CTTGGGATTT ACGTCAAGTC CGTTGTGAAA 
GGAGGCGCTG CGGACGTGGA CGGGCGGCTC GCCGCGGGTG ACCAGCTGCT CAGCGTGGAC 
GGACGGAGTC TGGTGGGACT CTCTCAAGAA AGGGCAGCAG AACTGATGAC AAGGACAAGC 
TCCGTCGTGA CGCTTGAAGT AGCAAAGCAA GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CTTCCCCTAT GATGCAGAGA ATTTCAGAGC GCCGTGGGTC AGGTAAACCC 
CGACCAAAAA GCGAAGGCTT TGAGCTGTAC AACAACTCGG CTCAGAACGG GTCCCCGGAG 
AGCCCGCAGA CGCCCTGGGC CGAGTACAGC GAGCCCAAGA AGCTGCCGGG CGATGACCGG 
CTGACGAAGC ACAGGGCAGA CCACCGCTCC AGCCCCAACG TCGCAAATCA GCCTCCCAGT 
CCTGGCGGGA AGAGTGCGTA TGCCTCCGGA ACAGCAGCCA AGATCACATC TGTCTCCACG 
GGGAACCTCT GCGCTGAGGA GCAGACGCCT CCGCCCCGCC CCGAAGCCTA CCCCATCCCC 
ACGCAGACGT CCACCAGGGA GTACTTCACC TTCCCGGCCT CCAAGTCCCA GGACCGCGTG 
GCGCCCCCGC AGAGCCAGTG GCTGGGTTTC GAGGAGAAGC CCCACGTGCT GGCAGACGGA 
AACCACTCCA GCGCTGCCGT CCAGCGTGTG ACCCGCTCCC AGGAGGAGCT CCGCGAGGAC 
GCCTACCACC CCGAGCGGCA GCGGCCGGAG GCAGCCGCGG ATCGCAGGTC GGATGGCAGC 
GACGCCTGGC CCACCCAGGG CTCATCACTG GGTTCCGGCG CGTCCAGCCA GGAGCACCTG 
GGGCCCCCCT CCAAGGCGGG CACCCCCGCC TCCACGCTCA CCAAGAGCGG CCCCGGGCGC 
TGGAAGACCC CGGCCGCCTC CCCGCCCATG CGGACCGACC CGGCCCTGCC GCCACCGCCG 
CCCCCCGGGT CCTACGCCGG GGAGGCTGAC GCGGACGCGG ACTCGCCCCC GCCTCCCGCC 
GCCACCGCCG CCGGCCAGGC GCAGGCGGCC GCCGAGCGCA AGAAACGAGA GGAGCAGCAG 
CGCTGGTACG AGAAGGAAAA AGCGCGCCTG GACGAGGAGC GCGAGCGCCG GCGCCGGGAG 
CAGGAGCGCC GGCTGGGCCA GCTGCGCGCG CCCGCGCCGC CCCCCGCGCC CGCCCGCGCG 
CCCGACACGG TGATCCGCGA GCTGCAGCCG CAGCAGCAGC CGCGTACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACGGT CAGCAGGGAG GAGCTGGCCT CCGGAGACAG CCTGTCCCCC 
GACCCCTGGA AGCGCGATGC GCGCGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTG 
GACCTGCTGA GCCGCGAGAT CCAGGAGCTG CAGGGCAAGG CCGAGCGCTC GGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT GCTGCTGGAG TGGCAGTTCC AGAAGCGGCT GCAGGAGTCC 
AAGCAGAAGG ACGAGGACGA CGAGGAGGAG GAGGACGATG ACGTGGACAC CGTGCTGCTC 
ATGCAGCGCC TGGAGGCCGA GCGCAGAGCG CGGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGGTCCTGGT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807060.1
CDS	54..4790
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKVLVL

Target ORF information:

RefSeq Version	XM_010808758.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808758.2

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GGGAGAAGGA ACCACTCGGC CTGCTACAGC 
TCTCACGCTT CCGAAGACGG TTCTGACTCC AGAGACAAGC CAAAGCTCTA CCGCCTTCAG 
CTCAGCGTCA CGGAGGTGGG GACCGAGAAG TTCGACGACA GCTCCATCCA GTTGTTTGGT 
TCAGGCATTC AGCCCCATCA CTGTGACCTC ACGAATATGG ACGGGGTGGT CACGGTCACG 
CCGAGAAGCA TGGACGCTGA GACCTACGTG GATGGGCAGC GCATCTCGGA GACCACGATG 
CTGCAGAGCG GGATGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT CGTGGACCCC 
AGCCAGGACC ACACGCTCCC CAAGAGATCC GTGGACGGGG GCCTGACTGT GAAGGGCCCC 
AGACATAAGT CTGGAATTGT TCAGGAGACA ACTTTTGATT TAGGAGGAGA CGTTCACAGT 
GGAATGGCCT TACCAGCGAG CAAGAGCACC GCCAGGCTGG ACAGCGACAG GACGCCGTCC 
ACCTCCAGCA CCGCCGAGCG GGGGATGGTG AAGCCGATGG TCCGCGTGGA GCAGCAGCTG 
GACTACCGCC GGCAGGACAG CAGAACTCAG GATGCTCCCG GGCCTGACCT GATTCTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCCTTTT TATCTGCCAT TATAAATTAC 
ACTAATAGCT CTACAGTCCA TTTTAAGTTG TCGCCCACTT ACGTGTTATA CATGGCCTGT 
CGGTATGTAT TGTCCAGCCA GTACAGACCC GACGTCAGCC CCGCGGAACG CACGCACAAA 
GTCATGGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GGGTGATCCA GAAACAGAAG 
AACATTGCAG GGGCCCTTGC CTTCTGGATG GCCAACGCGT CCGAGCTGCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGGATCACA CTGGATGCAC AGGACGTCCT GGCACACTTG 
GTGCAGATGG CCTTTAAGTA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA TAATTACATG 
CCCGCTTTTC TGGACGACCC CGAAGAGGAC AGTCTGCAGA GACCAAAAAT AGACGACGTG 
CTGCACACGC TCACGGGCGC CATGTCCCTG CTGCGTCGCT GCCGGGTCAA TGCCGCGCTG 
ACCATCCAGC TCTTCTCCCA ACTCTTCCAC TTCATCAACA TGTGGCTGTT CAACCGGCTG 
GTGACCGACC CCGAGTCGGG GCTCTGCTCC CACTACTGGG GTGCCATCCT CCGCCAGCAG 
CTCGGGCACG TGGAGGCCTG GGCCGAGAAG CAGGGCCTGG AGCTGGCGGC TGACTGCCAC 
CTGAGCCGGA TAGTGCAGGC AACCACTTTG CTTACGATGG ATAAGTACGC ACCTGATGAC 
ATTCCAAATA TAAACAGCAC CTGCTTCAAG TTAAATTCAC TGCAGCTCCA AGCCTTACTG 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTGTCCCTG CGGACCTGAT CGCGAACGTG 
GTGGCCGTGG CCGAGAACAC AGCAGACGAG CTGGCACGCA GCGACGGGCG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCT TTCCTCCTGC CTGAGGACGG CTACTCCTGT 
GACGTCGTCA GGAATGTCCC CAGCGGCCTG CAGGAGTTCC TGGACCCTCT CTGCCAGAGA 
GGGTTTTGCA GGTTAATTCC TCACCCACGC TCTCCAGGCA CCTGGACGAT ATATTTTGAA 
GGTGCAGATT ACGAGAGTCA CCTTTTGCGG GAGAACACAG AACTGGCACA GCCTCTGCGT 
AAAGAACCTG AAATCATCAC TGTGACCCTC AAAAAGCAGA ACGGAATGGG CCTTAGCATT 
GTTGCTGCAA AGGGCGCTGG TCAAGACAAA CTTGGGATTT ACGTCAAGTC CGTTGTGAAA 
GGAGGCGCTG CGGACGTGGA CGGGCGGCTC GCCGCGGGTG ACCAGCTGCT CAGCGTGGAC 
GGACGGAGTC TGGTGGGACT CTCTCAAGAA AGGGCAGCAG AACTGATGAC AAGGACAAGC 
TCCGTCGTGA CGCTTGAAGT AGCAAAGCAA GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CTTCCCCTAT GATGCAGAGA ATTTCAGAGC GCCGTGGGTC AGGTAAACCC 
CGACCAAAAA GCGAAGGCTT TGAGCTGTAC AACAACTCGG CTCAGAACGG GTCCCCGGAG 
AGCCCGCAGA CGCCCTGGGC CGAGTACAGC GAGCCCAAGA AGCTGCCGGG CGATGACCGG 
CTGACGAAGC ACAGGGCAGA CCACCGCTCC AGCCCCAACG TCGCAAATCA GCCTCCCAGT 
CCTGGCGGGA AGAGTGCGTA TGCCTCCGGA ACAGCAGCCA AGATCACATC TGTCTCCACG 
GGGAACCTCT GCGCTGAGGA GCAGACGCCT CCGCCCCGCC CCGAAGCCTA CCCCATCCCC 
ACGCAGACGT CCACCAGGGA GTACTTCACC TTCCCGGCCT CCAAGTCCCA GGACCGCGTG 
GCGCCCCCGC AGAGCCAGTG GCTGGGTTTC GAGGAGAAGC CCCACGTGCT GGCAGACGGA 
AACCACTCCA GCGCTGCCGT CCAGCGTGTG ACCCGCTCCC AGGAGGAGCT CCGCGAGGAC 
GCCTACCACC CCGAGCGGCA GCGGCCGGAG GCAGCCGCGG ATCGCAGGTC GGATGGCAGC 
GACGCCTGGC CCACCCAGGG CTCATCACTG GGTTCCGGCG CGTCCAGCCA GGAGCACCTG 
GGGCCCCCCT CCAAGGCGGG CACCCCCGCC TCCACGCTCA CCAAGAGCGG CCCCGGGCGC 
TGGAAGACCC CGGCCGCCTC CCCGCCCATG CGGACCGACC CGGCCCTGCC GCCACCGCCG 
CCCCCCGGGT CCTACGCCGG GGAGGCTGAC GCGGACGCGG ACTCGCCCCC GCCTCCCGCC 
GCCACCGCCG CCGGCCAGGC GCAGGCGGCC GCCGAGCGCA AGAAACGAGA GGAGCAGCAG 
CGCTGGTACG AGAAGGAAAA AGCGCGCCTG GACGAGGAGC GCGAGCGCCG GCGCCGGGAG 
CAGGAGCGCC GGCTGGGCCA GCTGCGCGCG CCCGCGCCGC CCCCCGCGCC CGCCCGCGCG 
CCCGACACGG TGATCCGCGA GCTGCAGCCG CAGCAGCAGC CGCGTACCAT CGAGCGCAGG 
GACCTGCAGT ACATCACGGT CAGCAGGGAG GAGCTGGCCT CCGGAGACAG CCTGTCCCCC 
GACCCCTGGA AGCGCGATGC GCGCGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTG 
GACCTGCTGA GCCGCGAGAT CCAGGAGCTG CAGGGCAAGG CCGAGCGCTC GGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT GCTGCTGGAG TGGCAGTTCC AGAAGCGGCT GCAGGAGTCC 
AAGCAGAAGG ACGAGGACGA CGAGGAGGAG GAGGACGATG ACGTGGACAC CGTGCTGCTC 
ATGCAGCGCC TGGAGGCCGA GCGCAGAGCG CGGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGGTCCTGGT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472941.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328427.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

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CloneID	OBa91541
Clone ID Related Accession (Same CDS sequence)	XM_015472941.1
Accession Version	XM_015472941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GACGGTTCTG ACTCCAGAGA CAAGCCAAAG 
CTCTACCGCC TTCAGCTCAG CGTCACGGAG GTGGGGACCG AGAAGTTCGA CGACAGCTCC 
ATCCAGTTGT TTGGTTCAGG CATTCAGCCC CATCACTGTG ACCTCACGAA TATGGACGGG 
GTGGTCACGG TCACGCCGAG AAGCATGGAC GCTGAGACCT ACGTGGATGG GCAGCGCATC 
TCGGAGACCA CGATGCTGCA GAGCGGGATG AAGGTGCAGT TCGGGGCGTC CCACGTGTTC 
AAGTTCGTGG ACCCCAGCCA GGACCACACG CTCCCCAAGA GATCCGTGGA CGGGGGCCTG 
ACTGTGAAGG GCCCCAGACA TAAGTCTGGA ATTGTTCAGG AGACAACTTT TGATTTAGGA 
GGAGACGTTC ACAGTGGAAT GGCCTTACCA GCGAGCAAGA GCACCGCCAG GCTGGACAGC 
GACAGGACGC CGTCCACCTC CAGCACCGCC GAGCGGGGGA TGGTGAAGCC GATGGTCCGC 
GTGGAGCAGC AGCTGGACTA CCGCCGGCAG GACAGCAGAA CTCAGGATGC TCCCGGGCCT 
GACCTGATTC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC CTTTTTATCT 
GCCATTATAA ATTACACTAA TAGCTCTACA GTCCATTTTA AGTTGTCGCC CACTTACGTG 
TTATACATGG CCTGTCGGTA TGTATTGTCC AGCCAGTACA GACCCGACGT CAGCCCCGCG 
GAACGCACGC ACAAAGTCAT GGCCATCGTC AACAAGATGG TGAGCATGAT GGAAGGGGTG 
ATCCAGGAAG TAGACCAGGT TGATCAGAAA CAGAAGAACA TTGCAGGGGC CCTTGCCTTC 
TGGATGGCCA ACGCGTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTCAGTCGG 
ATCACACTGG ATGCACAGGA CGTCCTGGCA CACTTGGTGC AGATGGCCTT TAAGTACCTG 
GTTCACTGTC TTCAGTCAGA GCTGAATAAT TACATGCCCG CTTTTCTGGA CGACCCCGAA 
GAGGACAGTC TGCAGAGACC AAAAATAGAC GACGTGCTGC ACACGCTCAC GGGCGCCATG 
TCCCTGCTGC GTCGCTGCCG GGTCAATGCC GCGCTGACCA TCCAGCTCTT CTCCCAACTC 
TTCCACTTCA TCAACATGTG GCTGTTCAAC CGGCTGGTGA CCGACCCCGA GTCGGGGCTC 
TGCTCCCACT ACTGGGGTGC CATCCTCCGC CAGCAGCTCG GGCACGTGGA GGCCTGGGCC 
GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC TGCCACCTGA GCCGGATAGT GCAGGCAACC 
ACTTTGCTTA CGATGGATAA GTACGCACCT GATGACATTC CAAATATAAA CAGCACCTGC 
TTCAAGTTAA ATTCACTGCA GCTCCAAGCC TTACTGCAGA ATTACCACTG TGCACCCGAT 
GAGCCTTTTG TCCCTGCGGA CCTGATCGCG AACGTGGTGG CCGTGGCCGA GAACACAGCA 
GACGAGCTGG CACGCAGCGA CGGGCGGGAG GTGCGGCTGG AGGAGGACCC TGACCTGCAG 
CTGCCTTTCC TCCTGCCTGA GGACGGCTAC TCCTGTGACG TCGTCAGGAA TGTCCCCAGC 
GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC CAGAGAGGGT TTTGCAGGTT AATTCCTCAC 
CCACGCTCTC CAGGCACCTG GACGATATAT TTTGAAGGTG CAGATTACGA GAGTCACCTT 
TTGCGGGAGA ACACAGAACT GGCACAGCCT CTGCGTAAAG AACCTGAAAT CATCACTGTG 
ACCCTCAAAA AGCAGAACGG AATGGGCCTT AGCATTGTTG CTGCAAAGGG CGCTGGTCAA 
GACAAACTTG GGATTTACGT CAAGTCCGTT GTGAAAGGAG GCGCTGCGGA CGTGGACGGG 
CGGCTCGCCG CGGGTGACCA GCTGCTCAGC GTGGACGGAC GGAGTCTGGT GGGACTCTCT 
CAAGAAAGGG CAGCAGAACT GATGACAAGG ACAAGCTCCG TCGTGACGCT TGAAGTAGCA 
AAGCAAGGAG CCATTTACCA CGGCCTCGCC ACCCTGCTCA ACCAGCCTTC CCCTATGATG 
CAGAGAATTT CAGAGCGCCG TGGGTCAGGT AAACCCCGAC CAAAAAGCGA AGGCTTTGAG 
CTGTACAACA ACTCGGCTCA GAACGGGTCC CCGGAGAGCC CGCAGACGCC CTGGGCCGAG 
TACAGCGAGC CCAAGAAGCT GCCGGGCGAT GACCGGCTGA CGAAGCACAG GGCAGACCAC 
CGCTCCAGCC CCAACGTCGC AAATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328427.1
CDS	56..5299
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472941.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472941.1

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GACGGTTCTG ACTCCAGAGA CAAGCCAAAG 
CTCTACCGCC TTCAGCTCAG CGTCACGGAG GTGGGGACCG AGAAGTTCGA CGACAGCTCC 
ATCCAGTTGT TTGGTTCAGG CATTCAGCCC CATCACTGTG ACCTCACGAA TATGGACGGG 
GTGGTCACGG TCACGCCGAG AAGCATGGAC GCTGAGACCT ACGTGGATGG GCAGCGCATC 
TCGGAGACCA CGATGCTGCA GAGCGGGATG AAGGTGCAGT TCGGGGCGTC CCACGTGTTC 
AAGTTCGTGG ACCCCAGCCA GGACCACACG CTCCCCAAGA GATCCGTGGA CGGGGGCCTG 
ACTGTGAAGG GCCCCAGACA TAAGTCTGGA ATTGTTCAGG AGACAACTTT TGATTTAGGA 
GGAGACGTTC ACAGTGGAAT GGCCTTACCA GCGAGCAAGA GCACCGCCAG GCTGGACAGC 
GACAGGACGC CGTCCACCTC CAGCACCGCC GAGCGGGGGA TGGTGAAGCC GATGGTCCGC 
GTGGAGCAGC AGCTGGACTA CCGCCGGCAG GACAGCAGAA CTCAGGATGC TCCCGGGCCT 
GACCTGATTC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC CTTTTTATCT 
GCCATTATAA ATTACACTAA TAGCTCTACA GTCCATTTTA AGTTGTCGCC CACTTACGTG 
TTATACATGG CCTGTCGGTA TGTATTGTCC AGCCAGTACA GACCCGACGT CAGCCCCGCG 
GAACGCACGC ACAAAGTCAT GGCCATCGTC AACAAGATGG TGAGCATGAT GGAAGGGGTG 
ATCCAGGAAG TAGACCAGGT TGATCAGAAA CAGAAGAACA TTGCAGGGGC CCTTGCCTTC 
TGGATGGCCA ACGCGTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTCAGTCGG 
ATCACACTGG ATGCACAGGA CGTCCTGGCA CACTTGGTGC AGATGGCCTT TAAGTACCTG 
GTTCACTGTC TTCAGTCAGA GCTGAATAAT TACATGCCCG CTTTTCTGGA CGACCCCGAA 
GAGGACAGTC TGCAGAGACC AAAAATAGAC GACGTGCTGC ACACGCTCAC GGGCGCCATG 
TCCCTGCTGC GTCGCTGCCG GGTCAATGCC GCGCTGACCA TCCAGCTCTT CTCCCAACTC 
TTCCACTTCA TCAACATGTG GCTGTTCAAC CGGCTGGTGA CCGACCCCGA GTCGGGGCTC 
TGCTCCCACT ACTGGGGTGC CATCCTCCGC CAGCAGCTCG GGCACGTGGA GGCCTGGGCC 
GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC TGCCACCTGA GCCGGATAGT GCAGGCAACC 
ACTTTGCTTA CGATGGATAA GTACGCACCT GATGACATTC CAAATATAAA CAGCACCTGC 
TTCAAGTTAA ATTCACTGCA GCTCCAAGCC TTACTGCAGA ATTACCACTG TGCACCCGAT 
GAGCCTTTTG TCCCTGCGGA CCTGATCGCG AACGTGGTGG CCGTGGCCGA GAACACAGCA 
GACGAGCTGG CACGCAGCGA CGGGCGGGAG GTGCGGCTGG AGGAGGACCC TGACCTGCAG 
CTGCCTTTCC TCCTGCCTGA GGACGGCTAC TCCTGTGACG TCGTCAGGAA TGTCCCCAGC 
GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC CAGAGAGGGT TTTGCAGGTT AATTCCTCAC 
CCACGCTCTC CAGGCACCTG GACGATATAT TTTGAAGGTG CAGATTACGA GAGTCACCTT 
TTGCGGGAGA ACACAGAACT GGCACAGCCT CTGCGTAAAG AACCTGAAAT CATCACTGTG 
ACCCTCAAAA AGCAGAACGG AATGGGCCTT AGCATTGTTG CTGCAAAGGG CGCTGGTCAA 
GACAAACTTG GGATTTACGT CAAGTCCGTT GTGAAAGGAG GCGCTGCGGA CGTGGACGGG 
CGGCTCGCCG CGGGTGACCA GCTGCTCAGC GTGGACGGAC GGAGTCTGGT GGGACTCTCT 
CAAGAAAGGG CAGCAGAACT GATGACAAGG ACAAGCTCCG TCGTGACGCT TGAAGTAGCA 
AAGCAAGGAG CCATTTACCA CGGCCTCGCC ACCCTGCTCA ACCAGCCTTC CCCTATGATG 
CAGAGAATTT CAGAGCGCCG TGGGTCAGGT AAACCCCGAC CAAAAAGCGA AGGCTTTGAG 
CTGTACAACA ACTCGGCTCA GAACGGGTCC CCGGAGAGCC CGCAGACGCC CTGGGCCGAG 
TACAGCGAGC CCAAGAAGCT GCCGGGCGAT GACCGGCTGA CGAAGCACAG GGCAGACCAC 
CGCTCCAGCC CCAACGTCGC AAATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472942.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328428.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

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CloneID	OBa91542
Clone ID Related Accession (Same CDS sequence)	XM_015472942.1
Accession Version	XM_015472942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GACGGTTCTG ACTCCAGAGA CAAGCCAAAG 
CTCTACCGCC TTCAGCTCAG CGTCACGGAG GTGGGGACCG AGAAGTTCGA CGACAGCTCC 
ATCCAGTTGT TTGGTTCAGG CATTCAGCCC CATCACTGTG ACCTCACGAA TATGGACGGG 
GTGGTCACGG TCACGCCGAG AAGCATGGAC GCTGAGACCT ACGTGGATGG GCAGCGCATC 
TCGGAGACCA CGATGCTGCA GAGCGGGATG AAGGTGCAGT TCGGGGCGTC CCACGTGTTC 
AAGTTCGTGG ACCCCAGCCA GGACCACACG CTCCCCAAGA GATCCGTGGA CGGGGGCCTG 
ACTGTGAAGG GCCCCAGACA TAAGTCTGGA ATTGTTCAGG AGACAACTTT TGATTTAGGA 
GGAGACGTTC ACAGTGGAAT GGCCTTACCA GCGAGCAAGA GCACCGCCAG GCTGGACAGC 
GACAGGACGC CGTCCACCTC CAGCACCGCC GAGCGGGGGA TGGTGAAGCC GATGGTCCGC 
GTGGAGCAGC AGCTGGACTA CCGCCGGCAG GACAGCAGAA CTCAGGATGC TCCCGGGCCT 
GACCTGATTC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC CTTTTTATCT 
GCCATTATAA ATTACACTAA TAGCTCTACA GTCCATTTTA AGTTGTCGCC CACTTACGTG 
TTATACATGG CCTGTCGGTA TGTATTGTCC AGCCAGTACA GACCCGACGT CAGCCCCGCG 
GAACGCACGC ACAAAGTCAT GGCCATCGTC AACAAGATGG TGAGCATGAT GGAAGGGGTG 
ATCCAGGAAG TAGACCAGGT TGATCAGAAA CAGAAGAACA TTGCAGGGGC CCTTGCCTTC 
TGGATGGCCA ACGCGTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTCAGTCGG 
ATCACACTGG ATGCACAGGA CGTCCTGGCA CACTTGGTGC AGATGGCCTT TAAGTACCTG 
GTTCACTGTC TTCAGTCAGA GCTGAATAAT TACATGCCCG CTTTTCTGGA CGACCCCGAA 
GAGGACAGTC TGCAGAGACC AAAAATAGAC GACGTGCTGC ACACGCTCAC GGGCGCCATG 
TCCCTGCTGC GTCGCTGCCG GGTCAATGCC GCGCTGACCA TCCAGCTCTT CTCCCAACTC 
TTCCACTTCA TCAACATGTG GCTGTTCAAC CGGCTGGTGA CCGACCCCGA GTCGGGGCTC 
TGCTCCCACT ACTGGGGTGC CATCCTCCGC CAGCAGCTCG GGCACGTGGA GGCCTGGGCC 
GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC TGCCACCTGA GCCGGATAGT GCAGGCAACC 
ACTTTGCTTA CGATGGATAA GTACGCACCT GATGACATTC CAAATATAAA CAGCACCTGC 
TTCAAGTTAA ATTCACTGCA GCTCCAAGCC TTACTGCAGA ATTACCACTG TGCACCCGAT 
GAGCCTTTTG TCCCTGCGGA CCTGATCGCG AACGTGGTGG CCGTGGCCGA GAACACAGCA 
GACGAGCTGG CACGCAGCGA CGGGCGGGAG GTGCGGCTGG AGGAGGACCC TGACCTGCAG 
CTGCCTTTCC TCCTGCCTGA GGACGGCTAC TCCTGTGACG TCGTCAGGAA TGTCCCCAGC 
GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC CAGAGAGGGT TTTGCAGGTT AATTCCTCAC 
CCACGCTCTC CAGGCACCTG GACGATATAT TTTGAAGGTG CAGATTACGA GAGTCACCTT 
TTGCGGGAGA ACACAGAACT GGCACAGCCT CTGCGTAAAG AACCTGAAAT CATCACTGTG 
ACCCTCAAAA AGCAGAACGG AATGGGCCTT AGCATTGTTG CTGCAAAGGG CGCTGGTCAA 
GACAAACTTG GGATTTACGT CAAGTCCGTT GTGAAAGGAG GCGCTGCGGA CGTGGACGGG 
CGGCTCGCCG CGGGTGACCA GCTGCTCAGC GTGGACGGAC GGAGTCTGGT GGGACTCTCT 
CAAGAAAGGG CAGCAGAACT GATGACAAGG ACAAGCTCCG TCGTGACGCT TGAAGTAGCA 
AAGCAAGGAG CCATTTACCA CGGCCTCGCC ACCCTGCTCA ACCAGCCTTC CCCTATGATG 
CAGAGAATTT CAGAGCGCCG TGGGTCAGGT AAACCCCGAC CAAAAAGCGA AGGCTTTGAG 
CTGTACAACA ACTCGGCTCA GAACGGGTCC CCGGAGAGCC CGCAGACGCC CTGGGCCGAG 
TACAGCGAGC CCAAGAAGCT GCCGGGCGAT GACCGGCTGA CGAAGCACAG GGCAGACCAC 
CGCTCCAGCC CCAACGTCGC AAATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328428.1
CDS	56..5299
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472942.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472942.1

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTCATCCAGA ACTTTAAGAG AACTCTCTCA 
AAGAAGGAGA AGAAGGAAAA AAAGAAGAGA GAAAAAGAGG CAGTGAGACA GGCGTCTGAG 
AAAGATGACA GACCTTTCTC TGGGGATGAC ACTGAGAATT CTCGCCTGGC CGCTGAGGTC 
TACAAAGACA TGCCTGAGAC CAGCTTTACG CGAACCATCT CCAACCCTGA GGTGGTCATG 
AAGCGGCGGC GGCAGCAGAA GCTGGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCAGACT CGGGTGGAAC ATTGAGAATT TACGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACGA TCCTGCTGTC CACTACAGAC CCTGCGGACT TTGCTGTGGC CGAAGCCTTG 
GAGAAGTACG GCCTGGAGAA GGAGAACCCC AGGGACTACT GCATCGCCCG TGTCATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAAAAA GGTGCTAAAG AAGTTATTCT TGATGATGAC 
GAATGTCCTT TACAGATCTT CAGGGAATGG CCGAGTGACA GAGGGATTTT AGTCTTTCAG 
TTGAAGAGGA GGCCACCAGA CCACATCCCA AAGAAAAGTA AGAAGCACAC GGACGGGAAG 
CCCCTGCAGG GGAAGGAGAG AGCCGATGGG TCGGCCTACG GCTCCGCCCT GCCCCCCGAG 
AAGCTGCCCT ACCTCGTGGA GCTGAGCCCG GACGGTTCTG ACTCCAGAGA CAAGCCAAAG 
CTCTACCGCC TTCAGCTCAG CGTCACGGAG GTGGGGACCG AGAAGTTCGA CGACAGCTCC 
ATCCAGTTGT TTGGTTCAGG CATTCAGCCC CATCACTGTG ACCTCACGAA TATGGACGGG 
GTGGTCACGG TCACGCCGAG AAGCATGGAC GCTGAGACCT ACGTGGATGG GCAGCGCATC 
TCGGAGACCA CGATGCTGCA GAGCGGGATG AAGGTGCAGT TCGGGGCGTC CCACGTGTTC 
AAGTTCGTGG ACCCCAGCCA GGACCACACG CTCCCCAAGA GATCCGTGGA CGGGGGCCTG 
ACTGTGAAGG GCCCCAGACA TAAGTCTGGA ATTGTTCAGG AGACAACTTT TGATTTAGGA 
GGAGACGTTC ACAGTGGAAT GGCCTTACCA GCGAGCAAGA GCACCGCCAG GCTGGACAGC 
GACAGGACGC CGTCCACCTC CAGCACCGCC GAGCGGGGGA TGGTGAAGCC GATGGTCCGC 
GTGGAGCAGC AGCTGGACTA CCGCCGGCAG GACAGCAGAA CTCAGGATGC TCCCGGGCCT 
GACCTGATTC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC CTTTTTATCT 
GCCATTATAA ATTACACTAA TAGCTCTACA GTCCATTTTA AGTTGTCGCC CACTTACGTG 
TTATACATGG CCTGTCGGTA TGTATTGTCC AGCCAGTACA GACCCGACGT CAGCCCCGCG 
GAACGCACGC ACAAAGTCAT GGCCATCGTC AACAAGATGG TGAGCATGAT GGAAGGGGTG 
ATCCAGGAAG TAGACCAGGT TGATCAGAAA CAGAAGAACA TTGCAGGGGC CCTTGCCTTC 
TGGATGGCCA ACGCGTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTCAGTCGG 
ATCACACTGG ATGCACAGGA CGTCCTGGCA CACTTGGTGC AGATGGCCTT TAAGTACCTG 
GTTCACTGTC TTCAGTCAGA GCTGAATAAT TACATGCCCG CTTTTCTGGA CGACCCCGAA 
GAGGACAGTC TGCAGAGACC AAAAATAGAC GACGTGCTGC ACACGCTCAC GGGCGCCATG 
TCCCTGCTGC GTCGCTGCCG GGTCAATGCC GCGCTGACCA TCCAGCTCTT CTCCCAACTC 
TTCCACTTCA TCAACATGTG GCTGTTCAAC CGGCTGGTGA CCGACCCCGA GTCGGGGCTC 
TGCTCCCACT ACTGGGGTGC CATCCTCCGC CAGCAGCTCG GGCACGTGGA GGCCTGGGCC 
GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC TGCCACCTGA GCCGGATAGT GCAGGCAACC 
ACTTTGCTTA CGATGGATAA GTACGCACCT GATGACATTC CAAATATAAA CAGCACCTGC 
TTCAAGTTAA ATTCACTGCA GCTCCAAGCC TTACTGCAGA ATTACCACTG TGCACCCGAT 
GAGCCTTTTG TCCCTGCGGA CCTGATCGCG AACGTGGTGG CCGTGGCCGA GAACACAGCA 
GACGAGCTGG CACGCAGCGA CGGGCGGGAG GTGCGGCTGG AGGAGGACCC TGACCTGCAG 
CTGCCTTTCC TCCTGCCTGA GGACGGCTAC TCCTGTGACG TCGTCAGGAA TGTCCCCAGC 
GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC CAGAGAGGGT TTTGCAGGTT AATTCCTCAC 
CCACGCTCTC CAGGCACCTG GACGATATAT TTTGAAGGTG CAGATTACGA GAGTCACCTT 
TTGCGGGAGA ACACAGAACT GGCACAGCCT CTGCGTAAAG AACCTGAAAT CATCACTGTG 
ACCCTCAAAA AGCAGAACGG AATGGGCCTT AGCATTGTTG CTGCAAAGGG CGCTGGTCAA 
GACAAACTTG GGATTTACGT CAAGTCCGTT GTGAAAGGAG GCGCTGCGGA CGTGGACGGG 
CGGCTCGCCG CGGGTGACCA GCTGCTCAGC GTGGACGGAC GGAGTCTGGT GGGACTCTCT 
CAAGAAAGGG CAGCAGAACT GATGACAAGG ACAAGCTCCG TCGTGACGCT TGAAGTAGCA 
AAGCAAGGAG CCATTTACCA CGGCCTCGCC ACCCTGCTCA ACCAGCCTTC CCCTATGATG 
CAGAGAATTT CAGAGCGCCG TGGGTCAGGT AAACCCCGAC CAAAAAGCGA AGGCTTTGAG 
CTGTACAACA ACTCGGCTCA GAACGGGTCC CCGGAGAGCC CGCAGACGCC CTGGGCCGAG 
TACAGCGAGC CCAAGAAGCT GCCGGGCGAT GACCGGCTGA CGAAGCACAG GGCAGACCAC 
CGCTCCAGCC CCAACGTCGC AAATCAGCCT CCCAGTCCTG GCGGGAAGAG TGCGTATGCC 
TCCGGAACAG CAGCCAAGAT CACATCTGTC TCCACGGGGA ACCTCTGCGC TGAGGAGCAG 
ACGCCTCCGC CCCGCCCCGA AGCCTACCCC ATCCCCACGC AGACGTCCAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAA GTCCCAGGAC CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG 
GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA GACGGAAACC ACTCCAGCGC TGCCGTCCAG 
CGTGTGACCC GCTCCCAGGA GGAGCTCCGC GAGGACGCCT ACCACCCCGA GCGGCAGCGG 
CCGGAGGCAG CCGCGGATCG CAGGTCGGAT GGCAGCGACG CCTGGCCCAC CCAGGGCTCA 
TCACTGGGTT CCGGCGCGTC CAGCCAGGAG CACCTGGGGC CCCCCTCCAA GGCGGGCACC 
CCCGCCTCCA CGCTCACCAA GAGCGGCCCC GGGCGCTGGA AGACCCCGGC CGCCTCCCCG 
CCCATGCGGA CCGACCCGGC CCTGCCGCCA CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG 
GCTGACGCGG ACGCGGACTC GCCCCCGCCT CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG 
GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG CAGCAGCGCT GGTACGAGAA GGAAAAAGCG 
CGCCTGGACG AGGAGCGCGA GCGCCGGCGC CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG 
CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC CGCGCGCCCG ACACGGTGAT CCGCGAGCTG 
CAGCCGCAGC AGCAGCCGCG TACCATCGAG CGCAGGGACC TGCAGTACAT CACGGTCAGC 
AGGGAGGAGC TGGCCTCCGG AGACAGCCTG TCCCCCGACC CCTGGAAGCG CGATGCGCGC 
GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC ATCGTGGACC TGCTGAGCCG CGAGATCCAG 
GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGATGACGT GGACACCGTG CTGCTCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGATGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGACACGGG AGGCGGAGGA GCGCGCGCGG 
CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC GAGCGGGTCA GGCGCGAGGC CGAAGACAAG 
AGGCGGCAGG AGGACGGCTA CTACAGCCGC CTGGAGGCCG AGCGGCGCCG ACAGCATGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC GCCACTCCCG 
CGCGGCTATG AGCCCGCCGG CCCGCCGCCA CCGCCCCAGC GCACCGCCTC CTACCTGCAG 
ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC ACCGCCAAGT TCGTGGCCTA CAATGAGGAG 
GAGGACGAGG ACGAAGAGCC GGCAGGAGCA AACTCTTACG TGGGACCTGC TGGGACAGTT 
TTTGGAGCCC ATGATGTTTA TCGGGACCCG AGAGAGAGAC CGCCTAAGAG CCACGAAGCA 
GATGTCCCCG GGGGTCCCGG AGCCCCTGAA AACCTGACGT TCAGGGAGCG CCAGCGCCTT 
TTCTCCCAAG GCCACGATGT CTCCAATAAA GTCAAGGCCT CTCGCAAGTT AACAGAGCTG 
GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472943.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328429.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

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CloneID	OBa91543
Clone ID Related Accession (Same CDS sequence)	XM_015472943.1
Accession Version	XM_015472943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGGA TGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG ACACGGGAGG CGGAGGAGCG CGCGCGGCAG 
GAGGAGCAGC GGCGCCGGCA GGAGGACGAG CGGGTCAGGC GCGAGGCCGA AGACAAGAGG 
CGGCAGGAGG ACGGCTACTA CAGCCGCCTG GAGGCCGAGC GGCGCCGACA GCATGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC 
GGCTATGAGC CCGCCGGCCC GCCGCCACCG CCCCAGCGCA CCGCCTCCTA CCTGCAGACG 
CAGGCCTTCT CCCCGGACGC CCTCTACACC GCCAAGTTCG TGGCCTACAA TGAGGAGGAG 
GACGAGGACG AAGAGCCGGC AGGAGCAAAC TCTTACGTGG GACCTGCTGG GACAGTTTTT 
GGAGCCCATG ATGTTTATCG GGACCCGAGA GAGAGACCGC CTAAGAGCCA CGAAGCAGAT 
GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC CTGACGTTCA GGGAGCGCCA GCGCCTTTTC 
TCCCAAGGCC ACGATGTCTC CAATAAAGTC AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG 
AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328429.1
CDS	56..5296
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472943.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472943.1

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGGA TGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG ACACGGGAGG CGGAGGAGCG CGCGCGGCAG 
GAGGAGCAGC GGCGCCGGCA GGAGGACGAG CGGGTCAGGC GCGAGGCCGA AGACAAGAGG 
CGGCAGGAGG ACGGCTACTA CAGCCGCCTG GAGGCCGAGC GGCGCCGACA GCATGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC 
GGCTATGAGC CCGCCGGCCC GCCGCCACCG CCCCAGCGCA CCGCCTCCTA CCTGCAGACG 
CAGGCCTTCT CCCCGGACGC CCTCTACACC GCCAAGTTCG TGGCCTACAA TGAGGAGGAG 
GACGAGGACG AAGAGCCGGC AGGAGCAAAC TCTTACGTGG GACCTGCTGG GACAGTTTTT 
GGAGCCCATG ATGTTTATCG GGACCCGAGA GAGAGACCGC CTAAGAGCCA CGAAGCAGAT 
GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC CTGACGTTCA GGGAGCGCCA GCGCCTTTTC 
TCCCAAGGCC ACGATGTCTC CAATAAAGTC AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG 
AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472944.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328430.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

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CloneID	OBa91544
Clone ID Related Accession (Same CDS sequence)	XM_015472944.1
Accession Version	XM_015472944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328430.1
CDS	56..5281
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472944.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472944.1

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472945.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328431.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

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CloneID	OBa91545
Clone ID Related Accession (Same CDS sequence)	XM_015472945.1
Accession Version	XM_015472945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTC ATCCAGAACT TTAAGAGAAC TCTCTCAAAG 
AAGGAGAAGA AGGAAAAAAA GAAGAGAGAA AAAGAGGCAG TGAGACAGGC GTCTGAGAAA 
GATGACAGAC CTTTCTCTGG GGATGACACT GAGAATTCTC GCCTGGCCGC TGAGGTCTAC 
AAAGACATGC CTGAGACCAG CTTTACGCGA ACCATCTCCA ACCCTGAGGT GGTCATGAAG 
CGGCGGCGGC AGCAGAAGCT GGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCAGACTCGG GTGGAACATT GAGAATTTAC GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACGATCC TGCTGTCCAC TACAGACCCT GCGGACTTTG CTGTGGCCGA AGCCTTGGAG 
AAGTACGGCC TGGAGAAGGA GAACCCCAGG GACTACTGCA TCGCCCGTGT CATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAAAAAGGT GCTAAAGAAG TTATTCTTGA TGATGACGAA 
TGTCCTTTAC AGATCTTCAG GGAATGGCCG AGTGACAGAG GGATTTTAGT CTTTCAGTTG 
AAGAGGAGGC CACCAGACCA CATCCCAAAG AAAAGTAAGA AGCACACGGA CGGGAAGCCC 
CTGCAGGGGA AGGAGAGAGC CGATGGGTCG GCCTACGGCT CCGCCCTGCC CCCCGAGAAG 
CTGCCCTACC TCGTGGAGCT GAGCCCGGAC GGTTCTGACT CCAGAGACAA GCCAAAGCTC 
TACCGCCTTC AGCTCAGCGT CACGGAGGTG GGGACCGAGA AGTTCGACGA CAGCTCCATC 
CAGTTGTTTG GTTCAGGCAT TCAGCCCCAT CACTGTGACC TCACGAATAT GGACGGGGTG 
GTCACGGTCA CGCCGAGAAG CATGGACGCT GAGACCTACG TGGATGGGCA GCGCATCTCG 
GAGACCACGA TGCTGCAGAG CGGGATGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTCGTGGACC CCAGCCAGGA CCACACGCTC CCCAAGAGAT CCGTGGACGG GGGCCTGACT 
GTGAAGGGCC CCAGACATAA GTCTGGAATT GTTCAGGAGA CAACTTTTGA TTTAGGAGGA 
GACGTTCACA GTGGAATGGC CTTACCAGCG AGCAAGAGCA CCGCCAGGCT GGACAGCGAC 
AGGACGCCGT CCACCTCCAG CACCGCCGAG CGGGGGATGG TGAAGCCGAT GGTCCGCGTG 
GAGCAGCAGC TGGACTACCG CCGGCAGGAC AGCAGAACTC AGGATGCTCC CGGGCCTGAC 
CTGATTCTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCCTT TTTATCTGCC 
ATTATAAATT ACACTAATAG CTCTACAGTC CATTTTAAGT TGTCGCCCAC TTACGTGTTA 
TACATGGCCT GTCGGTATGT ATTGTCCAGC CAGTACAGAC CCGACGTCAG CCCCGCGGAA 
CGCACGCACA AAGTCATGGC CATCGTCAAC AAGATGGTGA GCATGATGGA AGGGGTGATC 
CAGAAACAGA AGAACATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAACGC GTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGGATCA CACTGGATGC ACAGGACGTC 
CTGGCACACT TGGTGCAGAT GGCCTTTAAG TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AATAATTACA TGCCCGCTTT TCTGGACGAC CCCGAAGAGG ACAGTCTGCA GAGACCAAAA 
ATAGACGACG TGCTGCACAC GCTCACGGGC GCCATGTCCC TGCTGCGTCG CTGCCGGGTC 
AATGCCGCGC TGACCATCCA GCTCTTCTCC CAACTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACCGGC TGGTGACCGA CCCCGAGTCG GGGCTCTGCT CCCACTACTG GGGTGCCATC 
CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG 
GCTGACTGCC ACCTGAGCCG GATAGTGCAG GCAACCACTT TGCTTACGAT GGATAAGTAC 
GCACCTGATG ACATTCCAAA TATAAACAGC ACCTGCTTCA AGTTAAATTC ACTGCAGCTC 
CAAGCCTTAC TGCAGAATTA CCACTGTGCA CCCGATGAGC CTTTTGTCCC TGCGGACCTG 
ATCGCGAACG TGGTGGCCGT GGCCGAGAAC ACAGCAGACG AGCTGGCACG CAGCGACGGG 
CGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC 
GGCTACTCCT GTGACGTCGT CAGGAATGTC CCCAGCGGCC TGCAGGAGTT CCTGGACCCT 
CTCTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCACCCAC GCTCTCCAGG CACCTGGACG 
ATATATTTTG AAGGTGCAGA TTACGAGAGT CACCTTTTGC GGGAGAACAC AGAACTGGCA 
CAGCCTCTGC GTAAAGAACC TGAAATCATC ACTGTGACCC TCAAAAAGCA GAACGGAATG 
GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT GGTCAAGACA AACTTGGGAT TTACGTCAAG 
TCCGTTGTGA AAGGAGGCGC TGCGGACGTG GACGGGCGGC TCGCCGCGGG TGACCAGCTG 
CTCAGCGTGG ACGGACGGAG TCTGGTGGGA CTCTCTCAAG AAAGGGCAGC AGAACTGATG 
ACAAGGACAA GCTCCGTCGT GACGCTTGAA GTAGCAAAGC AAGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCTTCCCCT ATGATGCAGA GAATTTCAGA GCGCCGTGGG 
TCAGGTAAAC CCCGACCAAA AAGCGAAGGC TTTGAGCTGT ACAACAACTC GGCTCAGAAC 
GGGTCCCCGG AGAGCCCGCA GACGCCCTGG GCCGAGTACA GCGAGCCCAA GAAGCTGCCG 
GGCGATGACC GGCTGACGAA GCACAGGGCA GACCACCGCT CCAGCCCCAA CGTCGCAAAT 
CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG TATGCCTCCG GAACAGCAGC CAAGATCACA 
TCTGTCTCCA CGGGGAACCT CTGCGCTGAG GAGCAGACGC CTCCGCCCCG CCCCGAAGCC 
TACCCCATCC CCACGCAGAC GTCCACCAGG GAGTACTTCA CCTTCCCGGC CTCCAAGTCC 
CAGGACCGCG TGGCGCCCCC GCAGAGCCAG TGGCTGGGTT TCGAGGAGAA GCCCCACGTG 
CTGGCAGACG GAAACCACTC CAGCGCTGCC GTCCAGCGTG TGACCCGCTC CCAGGAGGAG 
CTCCGCGAGG ACGCCTACCA CCCCGAGCGG CAGCGGCCGG AGGCAGCCGC GGATCGCAGG 
TCGGATGGCA GCGACGCCTG GCCCACCCAG GGCTCATCAC TGGGTTCCGG CGCGTCCAGC 
CAGGAGCACC TGGGGCCCCC CTCCAAGGCG GGCACCCCCG CCTCCACGCT CACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC TCCCCGCCCA TGCGGACCGA CCCGGCCCTG 
CCGCCACCGC CGCCCCCCGG GTCCTACGCC GGGGAGGCTG ACGCGGACGC GGACTCGCCC 
CCGCCTCCCG CCGCCACCGC CGCCGGCCAG GCGCAGGCGG CCGCCGAGCG CAAGAAACGA 
GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA AAAGCGCGCC TGGACGAGGA GCGCGAGCGC 
CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG 
CCCGCCCGCG CGCCCGACAC GGTGATCCGC GAGCTGCAGC CGCAGCAGCA GCCGCGTACC 
ATCGAGCGCA GGGACCTGCA GTACATCACG GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAT GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG 
CTGCACATCG TGGACCTGCT GAGCCGCGAG ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC 
TCGGCCGAGG AGAGCGACCG GCTGCGCAAG CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA TGACGTGGAC 
ACCGTGCTGC TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTATGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAT GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGA CACGGGAGGC GGAGGAGCGC GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG 
GAGGACGAGC GGGTCAGGCG CGAGGCCGAA GACAAGAGGC GGCAGGAGGA CGGCTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCCGACAG CATGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA CTCCCGCGCG GCTATGAGCC CGCCGGCCCG 
CCGCCACCGC CCCAGCGCAC CGCCTCCTAC CTGCAGACGC AGGCCTTCTC CCCGGACGCC 
CTCTACACCG CCAAGTTCGT GGCCTACAAT GAGGAGGAGG ACGAGGACGA AGAGCCGGCA 
GGAGCAAACT CTTACGTGGG ACCTGCTGGG ACAGTTTTTG GAGCCCATGA TGTTTATCGG 
GACCCGAGAG AGAGACCGCC TAAGAGCCAC GAAGCAGATG TCCCCGGGGG TCCCGGAGCC 
CCTGAAAACC TGACGTTCAG GGAGCGCCAG CGCCTTTTCT CCCAAGGCCA CGATGTCTCC 
AATAAAGTCA AGGCCTCTCG CAAGTTAACA GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328431.1
CDS	54..5273
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472945.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472945.1

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGATGAGAA ACCCCTGGTG GTGCAGCTGA ACTGGAATAA AGATGACCGA 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GATGCTGTTC CTGCAAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTC ATCCAGAACT TTAAGAGAAC TCTCTCAAAG 
AAGGAGAAGA AGGAAAAAAA GAAGAGAGAA AAAGAGGCAG TGAGACAGGC GTCTGAGAAA 
GATGACAGAC CTTTCTCTGG GGATGACACT GAGAATTCTC GCCTGGCCGC TGAGGTCTAC 
AAAGACATGC CTGAGACCAG CTTTACGCGA ACCATCTCCA ACCCTGAGGT GGTCATGAAG 
CGGCGGCGGC AGCAGAAGCT GGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCAGACTCGG GTGGAACATT GAGAATTTAC GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACGATCC TGCTGTCCAC TACAGACCCT GCGGACTTTG CTGTGGCCGA AGCCTTGGAG 
AAGTACGGCC TGGAGAAGGA GAACCCCAGG GACTACTGCA TCGCCCGTGT CATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAAAAAGGT GCTAAAGAAG TTATTCTTGA TGATGACGAA 
TGTCCTTTAC AGATCTTCAG GGAATGGCCG AGTGACAGAG GGATTTTAGT CTTTCAGTTG 
AAGAGGAGGC CACCAGACCA CATCCCAAAG AAAAGTAAGA AGCACACGGA CGGGAAGCCC 
CTGCAGGGGA AGGAGAGAGC CGATGGGTCG GCCTACGGCT CCGCCCTGCC CCCCGAGAAG 
CTGCCCTACC TCGTGGAGCT GAGCCCGGAC GGTTCTGACT CCAGAGACAA GCCAAAGCTC 
TACCGCCTTC AGCTCAGCGT CACGGAGGTG GGGACCGAGA AGTTCGACGA CAGCTCCATC 
CAGTTGTTTG GTTCAGGCAT TCAGCCCCAT CACTGTGACC TCACGAATAT GGACGGGGTG 
GTCACGGTCA CGCCGAGAAG CATGGACGCT GAGACCTACG TGGATGGGCA GCGCATCTCG 
GAGACCACGA TGCTGCAGAG CGGGATGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTCGTGGACC CCAGCCAGGA CCACACGCTC CCCAAGAGAT CCGTGGACGG GGGCCTGACT 
GTGAAGGGCC CCAGACATAA GTCTGGAATT GTTCAGGAGA CAACTTTTGA TTTAGGAGGA 
GACGTTCACA GTGGAATGGC CTTACCAGCG AGCAAGAGCA CCGCCAGGCT GGACAGCGAC 
AGGACGCCGT CCACCTCCAG CACCGCCGAG CGGGGGATGG TGAAGCCGAT GGTCCGCGTG 
GAGCAGCAGC TGGACTACCG CCGGCAGGAC AGCAGAACTC AGGATGCTCC CGGGCCTGAC 
CTGATTCTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCCTT TTTATCTGCC 
ATTATAAATT ACACTAATAG CTCTACAGTC CATTTTAAGT TGTCGCCCAC TTACGTGTTA 
TACATGGCCT GTCGGTATGT ATTGTCCAGC CAGTACAGAC CCGACGTCAG CCCCGCGGAA 
CGCACGCACA AAGTCATGGC CATCGTCAAC AAGATGGTGA GCATGATGGA AGGGGTGATC 
CAGAAACAGA AGAACATTGC AGGGGCCCTT GCCTTCTGGA TGGCCAACGC GTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGGATCA CACTGGATGC ACAGGACGTC 
CTGGCACACT TGGTGCAGAT GGCCTTTAAG TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AATAATTACA TGCCCGCTTT TCTGGACGAC CCCGAAGAGG ACAGTCTGCA GAGACCAAAA 
ATAGACGACG TGCTGCACAC GCTCACGGGC GCCATGTCCC TGCTGCGTCG CTGCCGGGTC 
AATGCCGCGC TGACCATCCA GCTCTTCTCC CAACTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACCGGC TGGTGACCGA CCCCGAGTCG GGGCTCTGCT CCCACTACTG GGGTGCCATC 
CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG 
GCTGACTGCC ACCTGAGCCG GATAGTGCAG GCAACCACTT TGCTTACGAT GGATAAGTAC 
GCACCTGATG ACATTCCAAA TATAAACAGC ACCTGCTTCA AGTTAAATTC ACTGCAGCTC 
CAAGCCTTAC TGCAGAATTA CCACTGTGCA CCCGATGAGC CTTTTGTCCC TGCGGACCTG 
ATCGCGAACG TGGTGGCCGT GGCCGAGAAC ACAGCAGACG AGCTGGCACG CAGCGACGGG 
CGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC 
GGCTACTCCT GTGACGTCGT CAGGAATGTC CCCAGCGGCC TGCAGGAGTT CCTGGACCCT 
CTCTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCACCCAC GCTCTCCAGG CACCTGGACG 
ATATATTTTG AAGGTGCAGA TTACGAGAGT CACCTTTTGC GGGAGAACAC AGAACTGGCA 
CAGCCTCTGC GTAAAGAACC TGAAATCATC ACTGTGACCC TCAAAAAGCA GAACGGAATG 
GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT GGTCAAGACA AACTTGGGAT TTACGTCAAG 
TCCGTTGTGA AAGGAGGCGC TGCGGACGTG GACGGGCGGC TCGCCGCGGG TGACCAGCTG 
CTCAGCGTGG ACGGACGGAG TCTGGTGGGA CTCTCTCAAG AAAGGGCAGC AGAACTGATG 
ACAAGGACAA GCTCCGTCGT GACGCTTGAA GTAGCAAAGC AAGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCTTCCCCT ATGATGCAGA GAATTTCAGA GCGCCGTGGG 
TCAGGTAAAC CCCGACCAAA AAGCGAAGGC TTTGAGCTGT ACAACAACTC GGCTCAGAAC 
GGGTCCCCGG AGAGCCCGCA GACGCCCTGG GCCGAGTACA GCGAGCCCAA GAAGCTGCCG 
GGCGATGACC GGCTGACGAA GCACAGGGCA GACCACCGCT CCAGCCCCAA CGTCGCAAAT 
CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG TATGCCTCCG GAACAGCAGC CAAGATCACA 
TCTGTCTCCA CGGGGAACCT CTGCGCTGAG GAGCAGACGC CTCCGCCCCG CCCCGAAGCC 
TACCCCATCC CCACGCAGAC GTCCACCAGG GAGTACTTCA CCTTCCCGGC CTCCAAGTCC 
CAGGACCGCG TGGCGCCCCC GCAGAGCCAG TGGCTGGGTT TCGAGGAGAA GCCCCACGTG 
CTGGCAGACG GAAACCACTC CAGCGCTGCC GTCCAGCGTG TGACCCGCTC CCAGGAGGAG 
CTCCGCGAGG ACGCCTACCA CCCCGAGCGG CAGCGGCCGG AGGCAGCCGC GGATCGCAGG 
TCGGATGGCA GCGACGCCTG GCCCACCCAG GGCTCATCAC TGGGTTCCGG CGCGTCCAGC 
CAGGAGCACC TGGGGCCCCC CTCCAAGGCG GGCACCCCCG CCTCCACGCT CACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC TCCCCGCCCA TGCGGACCGA CCCGGCCCTG 
CCGCCACCGC CGCCCCCCGG GTCCTACGCC GGGGAGGCTG ACGCGGACGC GGACTCGCCC 
CCGCCTCCCG CCGCCACCGC CGCCGGCCAG GCGCAGGCGG CCGCCGAGCG CAAGAAACGA 
GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA AAAGCGCGCC TGGACGAGGA GCGCGAGCGC 
CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG 
CCCGCCCGCG CGCCCGACAC GGTGATCCGC GAGCTGCAGC CGCAGCAGCA GCCGCGTACC 
ATCGAGCGCA GGGACCTGCA GTACATCACG GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAT GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG 
CTGCACATCG TGGACCTGCT GAGCCGCGAG ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC 
TCGGCCGAGG AGAGCGACCG GCTGCGCAAG CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA TGACGTGGAC 
ACCGTGCTGC TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTATGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAT GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGA CACGGGAGGC GGAGGAGCGC GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG 
GAGGACGAGC GGGTCAGGCG CGAGGCCGAA GACAAGAGGC GGCAGGAGGA CGGCTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCCGACAG CATGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA CTCCCGCGCG GCTATGAGCC CGCCGGCCCG 
CCGCCACCGC CCCAGCGCAC CGCCTCCTAC CTGCAGACGC AGGCCTTCTC CCCGGACGCC 
CTCTACACCG CCAAGTTCGT GGCCTACAAT GAGGAGGAGG ACGAGGACGA AGAGCCGGCA 
GGAGCAAACT CTTACGTGGG ACCTGCTGGG ACAGTTTTTG GAGCCCATGA TGTTTATCGG 
GACCCGAGAG AGAGACCGCC TAAGAGCCAC GAAGCAGATG TCCCCGGGGG TCCCGGAGCC 
CCTGAAAACC TGACGTTCAG GGAGCGCCAG CGCCTTTTCT CCCAAGGCCA CGATGTCTCC 
AATAAAGTCA AGGCCTCTCG CAAGTTAACA GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472946.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328432.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

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CloneID	OBa91546
Clone ID Related Accession (Same CDS sequence)	XM_015472946.1
Accession Version	XM_015472946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGCT TCCTGCCAGG 
CTGTTTTGGT GGCGTGCGAC TTCTCCATCC TTCAGGGTAA AACAGCAAGC TCGTCTTCTC 
TCCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328432.1
CDS	56..4744
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLPARLFWWRATSPSFRVKQQARLLSI

Target ORF information:

RefSeq Version	XM_015472946.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472946.1

ORF Insert Sequence:

ATGAGGTTTT ATTTTCAAGA TAAAGCCGCC GGGAACTTCG CCACGAAATG CATCCGGGTG 
TCCAGCACCG CCACCACCCA GGACGTGCTG GAGACGCTGG CCGAGAAGTT CCGCCCCGAC 
ATGCGCATGC TGTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCCCTG GTGGTGCAGC TGAACTGGAA TAAAGATGAC 
CGAGAGGGCA GGTTTGTCCT TAAGAACGAG AACGATGCTG TTCCTGCAAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGG AAGTAGACCA GGTTGATCAG AAACAGAAGA ACATTGCAGG GGCCCTTGCC 
TTCTGGATGG CCAACGCGTC CGAGCTGCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGGATCACAC TGGATGCACA GGACGTCCTG GCACACTTGG TGCAGATGGC CTTTAAGTAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAT AATTACATGC CCGCTTTTCT GGACGACCCC 
GAAGAGGACA GTCTGCAGAG ACCAAAAATA GACGACGTGC TGCACACGCT CACGGGCGCC 
ATGTCCCTGC TGCGTCGCTG CCGGGTCAAT GCCGCGCTGA CCATCCAGCT CTTCTCCCAA 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACCGGCTGG TGACCGACCC CGAGTCGGGG 
CTCTGCTCCC ACTACTGGGG TGCCATCCTC CGCCAGCAGC TCGGGCACGT GGAGGCCTGG 
GCCGAGAAGC AGGGCCTGGA GCTGGCGGCT GACTGCCACC TGAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACGATGGA TAAGTACGCA CCTGATGACA TTCCAAATAT AAACAGCACC 
TGCTTCAAGT TAAATTCACT GCAGCTCCAA GCCTTACTGC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTGTCCCTGC GGACCTGATC GCGAACGTGG TGGCCGTGGC CGAGAACACA 
GCAGACGAGC TGGCACGCAG CGACGGGCGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCTT TCCTCCTGCC TGAGGACGGC TACTCCTGTG ACGTCGTCAG GAATGTCCCC 
AGCGGCCTGC AGGAGTTCCT GGACCCTCTC TGCCAGAGAG GGTTTTGCAG GTTAATTCCT 
CACCCACGCT CTCCAGGCAC CTGGACGATA TATTTTGAAG GTGCAGATTA CGAGAGTCAC 
CTTTTGCGGG AGAACACAGA ACTGGCACAG CCTCTGCGTA AAGAACCTGA AATCATCACT 
GTGACCCTCA AAAAGCAGAA CGGAATGGGC CTTAGCATTG TTGCTGCAAA GGGCGCTGGT 
CAAGACAAAC TTGGGATTTA CGTCAAGTCC GTTGTGAAAG GAGGCGCTGC GGACGTGGAC 
GGGCGGCTCG CCGCGGGTGA CCAGCTGCTC AGCGTGGACG GACGGAGTCT GGTGGGACTC 
TCTCAAGAAA GGGCAGCAGA ACTGATGACA AGGACAAGCT CCGTCGTGAC GCTTGAAGTA 
GCAAAGCAAG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC TTCCCCTATG 
ATGCAGAGAA TTTCAGAGCG CCGTGGGTCA GGTAAACCCC GACCAAAAAG CGAAGGCTTT 
GAGCTGTACA ACAACTCGGC TCAGAACGGG TCCCCGGAGA GCCCGCAGAC GCCCTGGGCC 
GAGTACAGCG AGCCCAAGAA GCTGCCGGGC GATGACCGGC TGACGAAGCA CAGGGCAGAC 
CACCGCTCCA GCCCCAACGT CGCAAATCAG CCTCCCAGTC CTGGCGGGAA GAGTGCGTAT 
GCCTCCGGAA CAGCAGCCAA GATCACATCT GTCTCCACGG GGAACCTCTG CGCTGAGGAG 
CAGACGCCTC CGCCCCGCCC CGAAGCCTAC CCCATCCCCA CGCAGACGTC CACCAGGGAG 
TACTTCACCT TCCCGGCCTC CAAGTCCCAG GACCGCGTGG CGCCCCCGCA GAGCCAGTGG 
CTGGGTTTCG AGGAGAAGCC CCACGTGCTG GCAGACGGAA ACCACTCCAG CGCTGCCGTC 
CAGCGTGTGA CCCGCTCCCA GGAGGAGCTC CGCGAGGACG CCTACCACCC CGAGCGGCAG 
CGGCCGGAGG CAGCCGCGGA TCGCAGGTCG GATGGCAGCG ACGCCTGGCC CACCCAGGGC 
TCATCACTGG GTTCCGGCGC GTCCAGCCAG GAGCACCTGG GGCCCCCCTC CAAGGCGGGC 
ACCCCCGCCT CCACGCTCAC CAAGAGCGGC CCCGGGCGCT GGAAGACCCC GGCCGCCTCC 
CCGCCCATGC GGACCGACCC GGCCCTGCCG CCACCGCCGC CCCCCGGGTC CTACGCCGGG 
GAGGCTGACG CGGACGCGGA CTCGCCCCCG CCTCCCGCCG CCACCGCCGC CGGCCAGGCG 
CAGGCGGCCG CCGAGCGCAA GAAACGAGAG GAGCAGCAGC GCTGGTACGA GAAGGAAAAA 
GCGCGCCTGG ACGAGGAGCG CGAGCGCCGG CGCCGGGAGC AGGAGCGCCG GCTGGGCCAG 
CTGCGCGCGC CCGCGCCGCC CCCCGCGCCC GCCCGCGCGC CCGACACGGT GATCCGCGAG 
CTGCAGCCGC AGCAGCAGCC GCGTACCATC GAGCGCAGGG ACCTGCAGTA CATCACGGTC 
AGCAGGGAGG AGCTGGCCTC CGGAGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGATGCG 
CGCGAGAAGT TGGAGAAGCA GCAGCAGCTG CACATCGTGG ACCTGCTGAG CCGCGAGATC 
CAGGAGCTGC AGGGCAAGGC CGAGCGCTCG GCCGAGGAGA GCGACCGGCT GCGCAAGCTG 
CTGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGATGA CGTGGACACC GTGCTGCTCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGCT TCCTGCCAGG 
CTGTTTTGGT GGCGTGCGAC TTCTCCATCC TTCAGGGTAA AACAGCAAGC TCGTCTTCTC 
TCCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472947.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328433.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

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CloneID	OBa91547
Clone ID Related Accession (Same CDS sequence)	XM_015472947.1
Accession Version	XM_015472947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCGA CCCCAGGGAG AAGGAACCAC TCGGCCTGCT ACAGCTCTCA CGCTTCCGAA 
GACGGTTCTG ACTCCAGAGA CAAGCCAAAG CTCTACCGCC TTCAGCTCAG CGTCACGGAG 
GTGGGGACCG AGAAGTTCGA CGACAGCTCC ATCCAGTTGT TTGGTTCAGG CATTCAGCCC 
CATCACTGTG ACCTCACGAA TATGGACGGG GTGGTCACGG TCACGCCGAG AAGCATGGAC 
GCTGAGACCT ACGTGGATGG GCAGCGCATC TCGGAGACCA CGATGCTGCA GAGCGGGATG 
AAGGTGCAGT TCGGGGCGTC CCACGTGTTC AAGTTCGTGG ACCCCAGCCA GGACCACACG 
CTCCCCAAGA GATCCGTGGA CGGGGGCCTG ACTGTGAAGG GCCCCAGACA TAAGTCTGGA 
ATTGTTCAGG AGACAACTTT TGATTTAGGA GGAGACGTTC ACAGTGGAAT GGCCTTACCA 
GCGAGCAAGA GCACCGCCAG GCTGGACAGC GACAGGACGC CGTCCACCTC CAGCACCGCC 
GAGCGGGGGA TGGTGAAGCC GATGGTCCGC GTGGAGCAGC AGCTGGACTA CCGCCGGCAG 
GACAGCAGAA CTCAGGATGC TCCCGGGCCT GACCTGATTC TGCCTGCAAG CATTGAATTC 
AGGGAAAGTT CTGAAGATTC CTTTTTATCT GCCATTATAA ATTACACTAA TAGCTCTACA 
GTCCATTTTA AGTTGTCGCC CACTTACGTG TTATACATGG CCTGTCGGTA TGTATTGTCC 
AGCCAGTACA GACCCGACGT CAGCCCCGCG GAACGCACGC ACAAAGTCAT GGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGGGTG ATCCAGGAAG TAGACCAGGT TGATCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGC AGACTGCTAT GCCAGCAATC 
TCTGTTCTAG ATTTGTTGCA GGATGAGGAG CGGCGGCGGC AGCAGCAGCT GGAGGAGATG 
CGGACACGGG AGGCGGAGGA GCGCGCGCGG CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC 
GAGCGGGTCA GGCGCGAGGC CGAAGACAAG AGGCGGCAGG AGGACGGCTA CTACAGCCGC 
CTGGAGGCCG AGCGGCGCCG ACAGCATGAG GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG 
GAGCCGGGCC TGGGCCGCCC GCCACTCCCG CGCGGCTATG AGCCCGCCGG CCCGCCGCCA 
CCGCCCCAGC GCACCGCCTC CTACCTGCAG ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC 
ACCGCCAAGT TCGTGGCCTA CAATGAGGAG GAGGACGAGG ACGAAGAGCC GGCAGGAGCA 
AACTCTTACG TGGGACCTGC TGGGACAGTT TTTGGAGCCC ATGATGTTTA TCGGGACCCG 
AGAGAGAGAC CGCCTAAGAG CCACGAAGCA GATGTCCCCG GGGGTCCCGG AGCCCCTGAA 
AACCTGACGT TCAGGGAGCG CCAGCGCCTT TTCTCCCAAG GCCACGATGT CTCCAATAAA 
GTCAAGGCCT CTCGCAAGTT AACAGAGCTG GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328433.1
CDS	115..4368
Translation	MTPTPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472947.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472947.1

ORF Insert Sequence:

ATGACTCCGA CCCCAGGGAG AAGGAACCAC TCGGCCTGCT ACAGCTCTCA CGCTTCCGAA 
GACGGTTCTG ACTCCAGAGA CAAGCCAAAG CTCTACCGCC TTCAGCTCAG CGTCACGGAG 
GTGGGGACCG AGAAGTTCGA CGACAGCTCC ATCCAGTTGT TTGGTTCAGG CATTCAGCCC 
CATCACTGTG ACCTCACGAA TATGGACGGG GTGGTCACGG TCACGCCGAG AAGCATGGAC 
GCTGAGACCT ACGTGGATGG GCAGCGCATC TCGGAGACCA CGATGCTGCA GAGCGGGATG 
AAGGTGCAGT TCGGGGCGTC CCACGTGTTC AAGTTCGTGG ACCCCAGCCA GGACCACACG 
CTCCCCAAGA GATCCGTGGA CGGGGGCCTG ACTGTGAAGG GCCCCAGACA TAAGTCTGGA 
ATTGTTCAGG AGACAACTTT TGATTTAGGA GGAGACGTTC ACAGTGGAAT GGCCTTACCA 
GCGAGCAAGA GCACCGCCAG GCTGGACAGC GACAGGACGC CGTCCACCTC CAGCACCGCC 
GAGCGGGGGA TGGTGAAGCC GATGGTCCGC GTGGAGCAGC AGCTGGACTA CCGCCGGCAG 
GACAGCAGAA CTCAGGATGC TCCCGGGCCT GACCTGATTC TGCCTGCAAG CATTGAATTC 
AGGGAAAGTT CTGAAGATTC CTTTTTATCT GCCATTATAA ATTACACTAA TAGCTCTACA 
GTCCATTTTA AGTTGTCGCC CACTTACGTG TTATACATGG CCTGTCGGTA TGTATTGTCC 
AGCCAGTACA GACCCGACGT CAGCCCCGCG GAACGCACGC ACAAAGTCAT GGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGGGTG ATCCAGGAAG TAGACCAGGT TGATCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGC AGACTGCTAT GCCAGCAATC 
TCTGTTCTAG ATTTGTTGCA GGATGAGGAG CGGCGGCGGC AGCAGCAGCT GGAGGAGATG 
CGGACACGGG AGGCGGAGGA GCGCGCGCGG CAGGAGGAGC AGCGGCGCCG GCAGGAGGAC 
GAGCGGGTCA GGCGCGAGGC CGAAGACAAG AGGCGGCAGG AGGACGGCTA CTACAGCCGC 
CTGGAGGCCG AGCGGCGCCG ACAGCATGAG GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG 
GAGCCGGGCC TGGGCCGCCC GCCACTCCCG CGCGGCTATG AGCCCGCCGG CCCGCCGCCA 
CCGCCCCAGC GCACCGCCTC CTACCTGCAG ACGCAGGCCT TCTCCCCGGA CGCCCTCTAC 
ACCGCCAAGT TCGTGGCCTA CAATGAGGAG GAGGACGAGG ACGAAGAGCC GGCAGGAGCA 
AACTCTTACG TGGGACCTGC TGGGACAGTT TTTGGAGCCC ATGATGTTTA TCGGGACCCG 
AGAGAGAGAC CGCCTAAGAG CCACGAAGCA GATGTCCCCG GGGGTCCCGG AGCCCCTGAA 
AACCTGACGT TCAGGGAGCG CCAGCGCCTT TTCTCCCAAG GCCACGATGT CTCCAATAAA 
GTCAAGGCCT CTCGCAAGTT AACAGAGCTG GAGAACGAGC TGAACACGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015472948.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328434.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

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CloneID	OBa91548
Clone ID Related Accession (Same CDS sequence)	XM_015472948.1
Accession Version	XM_015472948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCGA CCCCAGGGAG AAGGAACCAC TCGGCCTGCT ACAGCTCTCA CGCTTCCGAA 
GACGGTTCTG ACTCCAGAGA CAAGCCAAAG CTCTACCGCC TTCAGCTCAG CGTCACGGAG 
GTGGGGACCG AGAAGTTCGA CGACAGCTCC ATCCAGTTGT TTGGTTCAGG CATTCAGCCC 
CATCACTGTG ACCTCACGAA TATGGACGGG GTGGTCACGG TCACGCCGAG AAGCATGGAC 
GCTGAGACCT ACGTGGATGG GCAGCGCATC TCGGAGACCA CGATGCTGCA GAGCGGGATG 
AAGGTGCAGT TCGGGGCGTC CCACGTGTTC AAGTTCGTGG ACCCCAGCCA GGACCACACG 
CTCCCCAAGA GATCCGTGGA CGGGGGCCTG ACTGTGAAGG GCCCCAGACA TAAGTCTGGA 
ATTGTTCAGG AGACAACTTT TGATTTAGGA GGAGACGTTC ACAGTGGAAT GGCCTTACCA 
GCGAGCAAGA GCACCGCCAG GCTGGACAGC GACAGGACGC CGTCCACCTC CAGCACCGCC 
GAGCGGGGGA TGGTGAAGCC GATGGTCCGC GTGGAGCAGC AGCTGGACTA CCGCCGGCAG 
GACAGCAGAA CTCAGGATGC TCCCGGGCCT GACCTGATTC TGCCTGCAAG CATTGAATTC 
AGGGAAAGTT CTGAAGATTC CTTTTTATCT GCCATTATAA ATTACACTAA TAGCTCTACA 
GTCCATTTTA AGTTGTCGCC CACTTACGTG TTATACATGG CCTGTCGGTA TGTATTGTCC 
AGCCAGTACA GACCCGACGT CAGCCCCGCG GAACGCACGC ACAAAGTCAT GGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGGGTG ATCCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GATGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGACACGGGA GGCGGAGGAG 
CGCGCGCGGC AGGAGGAGCA GCGGCGCCGG CAGGAGGACG AGCGGGTCAG GCGCGAGGCC 
GAAGACAAGA GGCGGCAGGA GGACGGCTAC TACAGCCGCC TGGAGGCCGA GCGGCGCCGA 
CAGCATGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCG 
CCACTCCCGC GCGGCTATGA GCCCGCCGGC CCGCCGCCAC CGCCCCAGCG CACCGCCTCC 
TACCTGCAGA CGCAGGCCTT CTCCCCGGAC GCCCTCTACA CCGCCAAGTT CGTGGCCTAC 
AATGAGGAGG AGGACGAGGA CGAAGAGCCG GCAGGAGCAA ACTCTTACGT GGGACCTGCT 
GGGACAGTTT TTGGAGCCCA TGATGTTTAT CGGGACCCGA GAGAGAGACC GCCTAAGAGC 
CACGAAGCAG ATGTCCCCGG GGGTCCCGGA GCCCCTGAAA ACCTGACGTT CAGGGAGCGC 
CAGCGCCTTT TCTCCCAAGG CCACGATGTC TCCAATAAAG TCAAGGCCTC TCGCAAGTTA 
ACAGAGCTGG AGAACGAGCT GAACACGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328434.1
CDS	114..4346
Translation	MTPTPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015472948.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472948.1

ORF Insert Sequence:

ATGACTCCGA CCCCAGGGAG AAGGAACCAC TCGGCCTGCT ACAGCTCTCA CGCTTCCGAA 
GACGGTTCTG ACTCCAGAGA CAAGCCAAAG CTCTACCGCC TTCAGCTCAG CGTCACGGAG 
GTGGGGACCG AGAAGTTCGA CGACAGCTCC ATCCAGTTGT TTGGTTCAGG CATTCAGCCC 
CATCACTGTG ACCTCACGAA TATGGACGGG GTGGTCACGG TCACGCCGAG AAGCATGGAC 
GCTGAGACCT ACGTGGATGG GCAGCGCATC TCGGAGACCA CGATGCTGCA GAGCGGGATG 
AAGGTGCAGT TCGGGGCGTC CCACGTGTTC AAGTTCGTGG ACCCCAGCCA GGACCACACG 
CTCCCCAAGA GATCCGTGGA CGGGGGCCTG ACTGTGAAGG GCCCCAGACA TAAGTCTGGA 
ATTGTTCAGG AGACAACTTT TGATTTAGGA GGAGACGTTC ACAGTGGAAT GGCCTTACCA 
GCGAGCAAGA GCACCGCCAG GCTGGACAGC GACAGGACGC CGTCCACCTC CAGCACCGCC 
GAGCGGGGGA TGGTGAAGCC GATGGTCCGC GTGGAGCAGC AGCTGGACTA CCGCCGGCAG 
GACAGCAGAA CTCAGGATGC TCCCGGGCCT GACCTGATTC TGCCTGCAAG CATTGAATTC 
AGGGAAAGTT CTGAAGATTC CTTTTTATCT GCCATTATAA ATTACACTAA TAGCTCTACA 
GTCCATTTTA AGTTGTCGCC CACTTACGTG TTATACATGG CCTGTCGGTA TGTATTGTCC 
AGCCAGTACA GACCCGACGT CAGCCCCGCG GAACGCACGC ACAAAGTCAT GGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGGGTG ATCCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GATGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGACACGGGA GGCGGAGGAG 
CGCGCGCGGC AGGAGGAGCA GCGGCGCCGG CAGGAGGACG AGCGGGTCAG GCGCGAGGCC 
GAAGACAAGA GGCGGCAGGA GGACGGCTAC TACAGCCGCC TGGAGGCCGA GCGGCGCCGA 
CAGCATGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCG 
CCACTCCCGC GCGGCTATGA GCCCGCCGGC CCGCCGCCAC CGCCCCAGCG CACCGCCTCC 
TACCTGCAGA CGCAGGCCTT CTCCCCGGAC GCCCTCTACA CCGCCAAGTT CGTGGCCTAC 
AATGAGGAGG AGGACGAGGA CGAAGAGCCG GCAGGAGCAA ACTCTTACGT GGGACCTGCT 
GGGACAGTTT TTGGAGCCCA TGATGTTTAT CGGGACCCGA GAGAGAGACC GCCTAAGAGC 
CACGAAGCAG ATGTCCCCGG GGGTCCCGGA GCCCCTGAAA ACCTGACGTT CAGGGAGCGC 
CAGCGCCTTT TCTCCCAAGG CCACGATGTC TCCAATAAAG TCAAGGCCTC TCGCAAGTTA 
ACAGAGCTGG AGAACGAGCT GAACACGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997145.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852913.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OBa94637
Clone ID Related Accession (Same CDS sequence)	XM_024997145.1 , XM_015464898.1
Accession Version	XM_024997145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GATGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGACACGGGA GGCGGAGGAG 
CGCGCGCGGC AGGAGGAGCA GCGGCGCCGG CAGGAGGACG AGCGGGTCAG GCGCGAGGCC 
GAAGACAAGA GGCGGCAGGA GGACGGCTAC TACAGCCGCC TGGAGGCCGA GCGGCGCCGA 
CAGCATGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCG 
CCACTCCCGC GCGGCTATGA GCCCGCCGGC CCGCCGCCAC CGCCCCAGCG CACCGCCTCC 
TACCTGCAGA CGCAGGCCTT CTCCCCGGAC GCCCTCTACA CCGCCAAGTT CGTGGCCTAC 
AATGAGGAGG AGGACGAGGA CGAAGAGCCG GCAGGAGCAA ACTCTTACGT GGGACCTGCT 
GGGACAGTTT TTGGAGCCCA TGATGTTTAT CGGGACCCGA GAGAGAGACC GCCTAAGAGC 
CACGAAGCAG ATGTCCCCGG GGGTCCCGGA GCCCCTGAAA ACCTGACGTT CAGGGAGCGC 
CAGCGCCTTT TCTCCCAAGG CCACGATGTC TCCAATAAAG TCAAGGCCTC TCGCAAGTTA 
ACAGAGCTGG AGAACGAGCT GAACACGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852913.1
CDS	70..5442
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997145.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997145.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GATGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGACACGGGA GGCGGAGGAG 
CGCGCGCGGC AGGAGGAGCA GCGGCGCCGG CAGGAGGACG AGCGGGTCAG GCGCGAGGCC 
GAAGACAAGA GGCGGCAGGA GGACGGCTAC TACAGCCGCC TGGAGGCCGA GCGGCGCCGA 
CAGCATGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCG 
CCACTCCCGC GCGGCTATGA GCCCGCCGGC CCGCCGCCAC CGCCCCAGCG CACCGCCTCC 
TACCTGCAGA CGCAGGCCTT CTCCCCGGAC GCCCTCTACA CCGCCAAGTT CGTGGCCTAC 
AATGAGGAGG AGGACGAGGA CGAAGAGCCG GCAGGAGCAA ACTCTTACGT GGGACCTGCT 
GGGACAGTTT TTGGAGCCCA TGATGTTTAT CGGGACCCGA GAGAGAGACC GCCTAAGAGC 
CACGAAGCAG ATGTCCCCGG GGGTCCCGGA GCCCCTGAAA ACCTGACGTT CAGGGAGCGC 
CAGCGCCTTT TCTCCCAAGG CCACGATGTC TCCAATAAAG TCAAGGCCTC TCGCAAGTTA 
ACAGAGCTGG AGAACGAGCT GAACACGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464898.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320384.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X16, mRNA.

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CloneID	OBa94637
Clone ID Related Accession (Same CDS sequence)	XM_024997145.1 , XM_015464898.1
Accession Version	XM_015464898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GATGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGACACGGGA GGCGGAGGAG 
CGCGCGCGGC AGGAGGAGCA GCGGCGCCGG CAGGAGGACG AGCGGGTCAG GCGCGAGGCC 
GAAGACAAGA GGCGGCAGGA GGACGGCTAC TACAGCCGCC TGGAGGCCGA GCGGCGCCGA 
CAGCATGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCG 
CCACTCCCGC GCGGCTATGA GCCCGCCGGC CCGCCGCCAC CGCCCCAGCG CACCGCCTCC 
TACCTGCAGA CGCAGGCCTT CTCCCCGGAC GCCCTCTACA CCGCCAAGTT CGTGGCCTAC 
AATGAGGAGG AGGACGAGGA CGAAGAGCCG GCAGGAGCAA ACTCTTACGT GGGACCTGCT 
GGGACAGTTT TTGGAGCCCA TGATGTTTAT CGGGACCCGA GAGAGAGACC GCCTAAGAGC 
CACGAAGCAG ATGTCCCCGG GGGTCCCGGA GCCCCTGAAA ACCTGACGTT CAGGGAGCGC 
CAGCGCCTTT TCTCCCAAGG CCACGATGTC TCCAATAAAG TCAAGGCCTC TCGCAAGTTA 
ACAGAGCTGG AGAACGAGCT GAACACGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320384.1
CDS	63..5435
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464898.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464898.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GATGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGACACGGGA GGCGGAGGAG 
CGCGCGCGGC AGGAGGAGCA GCGGCGCCGG CAGGAGGACG AGCGGGTCAG GCGCGAGGCC 
GAAGACAAGA GGCGGCAGGA GGACGGCTAC TACAGCCGCC TGGAGGCCGA GCGGCGCCGA 
CAGCATGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCG 
CCACTCCCGC GCGGCTATGA GCCCGCCGGC CCGCCGCCAC CGCCCCAGCG CACCGCCTCC 
TACCTGCAGA CGCAGGCCTT CTCCCCGGAC GCCCTCTACA CCGCCAAGTT CGTGGCCTAC 
AATGAGGAGG AGGACGAGGA CGAAGAGCCG GCAGGAGCAA ACTCTTACGT GGGACCTGCT 
GGGACAGTTT TTGGAGCCCA TGATGTTTAT CGGGACCCGA GAGAGAGACC GCCTAAGAGC 
CACGAAGCAG ATGTCCCCGG GGGTCCCGGA GCCCCTGAAA ACCTGACGTT CAGGGAGCGC 
CAGCGCCTTT TCTCCCAAGG CCACGATGTC TCCAATAAAG TCAAGGCCTC TCGCAAGTTA 
ACAGAGCTGG AGAACGAGCT GAACACGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464899.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320385.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.

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CloneID	OBa94638
Clone ID Related Accession (Same CDS sequence)	XM_015464899.1
Accession Version	XM_015464899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAAT CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG 
TATGCCTCCG GAACAGCAGC CAAGATCACA TCTGTCTCCA CGGGGAACCT CTGCGCTGAG 
GAGCAGACGC CTCCGCCCCG CCCCGAAGCC TACCCCATCC CCACGCAGAC GTCCACCAGG 
GAGTACTTCA CCTTCCCGGC CTCCAAGTCC CAGGACCGCG TGGCGCCCCC GCAGAGCCAG 
TGGCTGGGTT TCGAGGAGAA GCCCCACGTG CTGGCAGACG GAAACCACTC CAGCGCTGCC 
GTCCAGCGTG TGACCCGCTC CCAGGAGGAG CTCCGCGAGG ACGCCTACCA CCCCGAGCGG 
CAGCGGCCGG AGGCAGCCGC GGATCGCAGG TCGGATGGCA GCGACGCCTG GCCCACCCAG 
GGCTCATCAC TGGGTTCCGG CGCGTCCAGC CAGGAGCACC TGGGGCCCCC CTCCAAGGCG 
GGCACCCCCG CCTCCACGCT CACCAAGAGC GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC 
TCCCCGCCCA TGCGGACCGA CCCGGCCCTG CCGCCACCGC CGCCCCCCGG GTCCTACGCC 
GGGGAGGCTG ACGCGGACGC GGACTCGCCC CCGCCTCCCG CCGCCACCGC CGCCGGCCAG 
GCGCAGGCGG CCGCCGAGCG CAAGAAACGA GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA 
AAAGCGCGCC TGGACGAGGA GCGCGAGCGC CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC 
CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG CCCGCCCGCG CGCCCGACAC GGTGATCCGC 
GAGCTGCAGC CGCAGCAGCA GCCGCGTACC ATCGAGCGCA GGGACCTGCA GTACATCACG 
GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC AGCCTGTCCC CCGACCCCTG GAAGCGCGAT 
GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG CTGCACATCG TGGACCTGCT GAGCCGCGAG 
ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC TCGGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG CTGCAGGAGT CCAAGCAGAA GGACGAGGAC 
GACGAGGAGG AGGAGGACGA TGACGTGGAC ACCGTGCTGC TCATGCAGCG CCTGGAGGCC 
GAGCGCAGAG CGCGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA 
CTCCCGCGCG GCTATGAGCC CGCCGGCCCG CCGCCACCGC CCCAGCGCAC CGCCTCCTAC 
CTGCAGACGC AGGCCTTCTC CCCGGACGCC CTCTACACCG CCAAGTTCGT GGCCTACAAT 
GAGGAGGAGG ACGAGGACGA AGAGCCGGCA GGAGCAAACT CTTACGTGGG ACCTGCTGGG 
ACAGTTTTTG GAGCCCATGA TGTTTATCGG GACCCGAGAG AGAGACCGCC TAAGAGCCAC 
GAAGCAGATG TCCCCGGGGG TCCCGGAGCC CCTGAAAACC TGACGTTCAG GGAGCGCCAG 
CGCCTTTTCT CCCAAGGCCA CGATGTCTCC AATAAAGTCA AGGCCTCTCG CAAGTTAACA 
GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320385.1
CDS	64..5433
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464899.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464899.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAAT CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG 
TATGCCTCCG GAACAGCAGC CAAGATCACA TCTGTCTCCA CGGGGAACCT CTGCGCTGAG 
GAGCAGACGC CTCCGCCCCG CCCCGAAGCC TACCCCATCC CCACGCAGAC GTCCACCAGG 
GAGTACTTCA CCTTCCCGGC CTCCAAGTCC CAGGACCGCG TGGCGCCCCC GCAGAGCCAG 
TGGCTGGGTT TCGAGGAGAA GCCCCACGTG CTGGCAGACG GAAACCACTC CAGCGCTGCC 
GTCCAGCGTG TGACCCGCTC CCAGGAGGAG CTCCGCGAGG ACGCCTACCA CCCCGAGCGG 
CAGCGGCCGG AGGCAGCCGC GGATCGCAGG TCGGATGGCA GCGACGCCTG GCCCACCCAG 
GGCTCATCAC TGGGTTCCGG CGCGTCCAGC CAGGAGCACC TGGGGCCCCC CTCCAAGGCG 
GGCACCCCCG CCTCCACGCT CACCAAGAGC GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC 
TCCCCGCCCA TGCGGACCGA CCCGGCCCTG CCGCCACCGC CGCCCCCCGG GTCCTACGCC 
GGGGAGGCTG ACGCGGACGC GGACTCGCCC CCGCCTCCCG CCGCCACCGC CGCCGGCCAG 
GCGCAGGCGG CCGCCGAGCG CAAGAAACGA GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA 
AAAGCGCGCC TGGACGAGGA GCGCGAGCGC CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC 
CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG CCCGCCCGCG CGCCCGACAC GGTGATCCGC 
GAGCTGCAGC CGCAGCAGCA GCCGCGTACC ATCGAGCGCA GGGACCTGCA GTACATCACG 
GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC AGCCTGTCCC CCGACCCCTG GAAGCGCGAT 
GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG CTGCACATCG TGGACCTGCT GAGCCGCGAG 
ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC TCGGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG CTGCAGGAGT CCAAGCAGAA GGACGAGGAC 
GACGAGGAGG AGGAGGACGA TGACGTGGAC ACCGTGCTGC TCATGCAGCG CCTGGAGGCC 
GAGCGCAGAG CGCGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA 
CTCCCGCGCG GCTATGAGCC CGCCGGCCCG CCGCCACCGC CCCAGCGCAC CGCCTCCTAC 
CTGCAGACGC AGGCCTTCTC CCCGGACGCC CTCTACACCG CCAAGTTCGT GGCCTACAAT 
GAGGAGGAGG ACGAGGACGA AGAGCCGGCA GGAGCAAACT CTTACGTGGG ACCTGCTGGG 
ACAGTTTTTG GAGCCCATGA TGTTTATCGG GACCCGAGAG AGAGACCGCC TAAGAGCCAC 
GAAGCAGATG TCCCCGGGGG TCCCGGAGCC CCTGAAAACC TGACGTTCAG GGAGCGCCAG 
CGCCTTTTCT CCCAAGGCCA CGATGTCTCC AATAAAGTCA AGGCCTCTCG CAAGTTAACA 
GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464900.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320386.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.

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CloneID	OBa94639
Clone ID Related Accession (Same CDS sequence)	XM_015464900.1
Accession Version	XM_015464900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAAT CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG 
TATGCCTCCG GAACAGCAGC CAAGATCACA TCTGTCTCCA CGGGGAACCT CTGCGCTGAG 
GAGCAGACGC CTCCGCCCCG CCCCGAAGCC TACCCCATCC CCACGCAGAC GTCCACCAGG 
GAGTACTTCA CCTTCCCGGC CTCCAAGTCC CAGGACCGCG TGGCGCCCCC GCAGAGCCAG 
TGGCTGGGTT TCGAGGAGAA GCCCCACGTG CTGGCAGACG GAAACCACTC CAGCGCTGCC 
GTCCAGCGTG TGACCCGCTC CCAGGAGGAG CTCCGCGAGG ACGCCTACCA CCCCGAGCGG 
CAGCGGCCGG AGGCAGCCGC GGATCGCAGG TCGGATGGCA GCGACGCCTG GCCCACCCAG 
GGCTCATCAC TGGGTTCCGG CGCGTCCAGC CAGGAGCACC TGGGGCCCCC CTCCAAGGCG 
GGCACCCCCG CCTCCACGCT CACCAAGAGC GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC 
TCCCCGCCCA TGCGGACCGA CCCGGCCCTG CCGCCACCGC CGCCCCCCGG GTCCTACGCC 
GGGGAGGCTG ACGCGGACGC GGACTCGCCC CCGCCTCCCG CCGCCACCGC CGCCGGCCAG 
GCGCAGGCGG CCGCCGAGCG CAAGAAACGA GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA 
AAAGCGCGCC TGGACGAGGA GCGCGAGCGC CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC 
CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG CCCGCCCGCG CGCCCGACAC GGTGATCCGC 
GAGCTGCAGC CGCAGCAGCA GCCGCGTACC ATCGAGCGCA GGGACCTGCA GTACATCACG 
GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC AGCCTGTCCC CCGACCCCTG GAAGCGCGAT 
GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG CTGCACATCG TGGACCTGCT GAGCCGCGAG 
ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC TCGGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG CTGCAGGAGT CCAAGCAGAA GGACGAGGAC 
GACGAGGAGG AGGAGGACGA TGACGTGGAC ACCGTGCTGC TCATGCAGCG CCTGGAGGCC 
GAGCGCAGAG CGCGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA 
CTCCCGCGCG GCTATGAGCC CGCCGGCCCG CCGCCACCGC CCCAGCGCAC CGCCTCCTAC 
CTGCAGACGC AGGCCTTCTC CCCGGACGCC CTCTACACCG CCAAGTTCGT GGCCTACAAT 
GAGGAGGAGG ACGAGGACGA AGAGCCGGCA GGAGCAAACT CTTACGTGGG ACCTGCTGGG 
ACAGTTTTTG GAGCCCATGA TGTTTATCGG GACCCGAGAG AGAGACCGCC TAAGAGCCAC 
GAAGCAGATG TCCCCGGGGG TCCCGGAGCC CCTGAAAACC TGACGTTCAG GGAGCGCCAG 
CGCCTTTTCT CCCAAGGCCA CGATGTCTCC AATAAAGTCA AGGCCTCTCG CAAGTTAACA 
GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320386.1
CDS	64..5433
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464900.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464900.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGACG GTTCTGACTC CAGAGACAAG 
CCAAAGCTCT ACCGCCTTCA GCTCAGCGTC ACGGAGGTGG GGACCGAGAA GTTCGACGAC 
AGCTCCATCC AGTTGTTTGG TTCAGGCATT CAGCCCCATC ACTGTGACCT CACGAATATG 
GACGGGGTGG TCACGGTCAC GCCGAGAAGC ATGGACGCTG AGACCTACGT GGATGGGCAG 
CGCATCTCGG AGACCACGAT GCTGCAGAGC GGGATGAAGG TGCAGTTCGG GGCGTCCCAC 
GTGTTCAAGT TCGTGGACCC CAGCCAGGAC CACACGCTCC CCAAGAGATC CGTGGACGGG 
GGCCTGACTG TGAAGGGCCC CAGACATAAG TCTGGAATTG TTCAGGAGAC AACTTTTGAT 
TTAGGAGGAG ACGTTCACAG TGGAATGGCC TTACCAGCGA GCAAGAGCAC CGCCAGGCTG 
GACAGCGACA GGACGCCGTC CACCTCCAGC ACCGCCGAGC GGGGGATGGT GAAGCCGATG 
GTCCGCGTGG AGCAGCAGCT GGACTACCGC CGGCAGGACA GCAGAACTCA GGATGCTCCC 
GGGCCTGACC TGATTCTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCCTTT 
TTATCTGCCA TTATAAATTA CACTAATAGC TCTACAGTCC ATTTTAAGTT GTCGCCCACT 
TACGTGTTAT ACATGGCCTG TCGGTATGTA TTGTCCAGCC AGTACAGACC CGACGTCAGC 
CCCGCGGAAC GCACGCACAA AGTCATGGCC ATCGTCAACA AGATGGTGAG CATGATGGAA 
GGGGTGATCC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAACATTGC AGGGGCCCTT 
GCCTTCTGGA TGGCCAACGC GTCCGAGCTG CTCAACTTCA TCAAGCAGGA CCGAGACCTC 
AGTCGGATCA CACTGGATGC ACAGGACGTC CTGGCACACT TGGTGCAGAT GGCCTTTAAG 
TACCTGGTTC ACTGTCTTCA GTCAGAGCTG AATAATTACA TGCCCGCTTT TCTGGACGAC 
CCCGAAGAGG ACAGTCTGCA GAGACCAAAA ATAGACGACG TGCTGCACAC GCTCACGGGC 
GCCATGTCCC TGCTGCGTCG CTGCCGGGTC AATGCCGCGC TGACCATCCA GCTCTTCTCC 
CAACTCTTCC ACTTCATCAA CATGTGGCTG TTCAACCGGC TGGTGACCGA CCCCGAGTCG 
GGGCTCTGCT CCCACTACTG GGGTGCCATC CTCCGCCAGC AGCTCGGGCA CGTGGAGGCC 
TGGGCCGAGA AGCAGGGCCT GGAGCTGGCG GCTGACTGCC ACCTGAGCCG GATAGTGCAG 
GCAACCACTT TGCTTACGAT GGATAAGTAC GCACCTGATG ACATTCCAAA TATAAACAGC 
ACCTGCTTCA AGTTAAATTC ACTGCAGCTC CAAGCCTTAC TGCAGAATTA CCACTGTGCA 
CCCGATGAGC CTTTTGTCCC TGCGGACCTG ATCGCGAACG TGGTGGCCGT GGCCGAGAAC 
ACAGCAGACG AGCTGGCACG CAGCGACGGG CGGGAGGTGC GGCTGGAGGA GGACCCTGAC 
CTGCAGCTGC CTTTCCTCCT GCCTGAGGAC GGCTACTCCT GTGACGTCGT CAGGAATGTC 
CCCAGCGGCC TGCAGGAGTT CCTGGACCCT CTCTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCACCCAC GCTCTCCAGG CACCTGGACG ATATATTTTG AAGGTGCAGA TTACGAGAGT 
CACCTTTTGC GGGAGAACAC AGAACTGGCA CAGCCTCTGC GTAAAGAACC TGAAATCATC 
ACTGTGACCC TCAAAAAGCA GAACGGAATG GGCCTTAGCA TTGTTGCTGC AAAGGGCGCT 
GGTCAAGACA AACTTGGGAT TTACGTCAAG TCCGTTGTGA AAGGAGGCGC TGCGGACGTG 
GACGGGCGGC TCGCCGCGGG TGACCAGCTG CTCAGCGTGG ACGGACGGAG TCTGGTGGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTGATG ACAAGGACAA GCTCCGTCGT GACGCTTGAA 
GTAGCAAAGC AAGGAGCCAT TTACCACGGC CTCGCCACCC TGCTCAACCA GCCTTCCCCT 
ATGATGCAGA GAATTTCAGA GCGCCGTGGG TCAGGTAAAC CCCGACCAAA AAGCGAAGGC 
TTTGAGCTGT ACAACAACTC GGCTCAGAAC GGGTCCCCGG AGAGCCCGCA GACGCCCTGG 
GCCGAGTACA GCGAGCCCAA GAAGCTGCCG GGCGATGACC GGCTGACGAA GCACAGGGCA 
GACCACCGCT CCAGCCCCAA CGTCGCAAAT CAGCCTCCCA GTCCTGGCGG GAAGAGTGCG 
TATGCCTCCG GAACAGCAGC CAAGATCACA TCTGTCTCCA CGGGGAACCT CTGCGCTGAG 
GAGCAGACGC CTCCGCCCCG CCCCGAAGCC TACCCCATCC CCACGCAGAC GTCCACCAGG 
GAGTACTTCA CCTTCCCGGC CTCCAAGTCC CAGGACCGCG TGGCGCCCCC GCAGAGCCAG 
TGGCTGGGTT TCGAGGAGAA GCCCCACGTG CTGGCAGACG GAAACCACTC CAGCGCTGCC 
GTCCAGCGTG TGACCCGCTC CCAGGAGGAG CTCCGCGAGG ACGCCTACCA CCCCGAGCGG 
CAGCGGCCGG AGGCAGCCGC GGATCGCAGG TCGGATGGCA GCGACGCCTG GCCCACCCAG 
GGCTCATCAC TGGGTTCCGG CGCGTCCAGC CAGGAGCACC TGGGGCCCCC CTCCAAGGCG 
GGCACCCCCG CCTCCACGCT CACCAAGAGC GGCCCCGGGC GCTGGAAGAC CCCGGCCGCC 
TCCCCGCCCA TGCGGACCGA CCCGGCCCTG CCGCCACCGC CGCCCCCCGG GTCCTACGCC 
GGGGAGGCTG ACGCGGACGC GGACTCGCCC CCGCCTCCCG CCGCCACCGC CGCCGGCCAG 
GCGCAGGCGG CCGCCGAGCG CAAGAAACGA GAGGAGCAGC AGCGCTGGTA CGAGAAGGAA 
AAAGCGCGCC TGGACGAGGA GCGCGAGCGC CGGCGCCGGG AGCAGGAGCG CCGGCTGGGC 
CAGCTGCGCG CGCCCGCGCC GCCCCCCGCG CCCGCCCGCG CGCCCGACAC GGTGATCCGC 
GAGCTGCAGC CGCAGCAGCA GCCGCGTACC ATCGAGCGCA GGGACCTGCA GTACATCACG 
GTCAGCAGGG AGGAGCTGGC CTCCGGAGAC AGCCTGTCCC CCGACCCCTG GAAGCGCGAT 
GCGCGCGAGA AGTTGGAGAA GCAGCAGCAG CTGCACATCG TGGACCTGCT GAGCCGCGAG 
ATCCAGGAGC TGCAGGGCAA GGCCGAGCGC TCGGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTGCTGCTGG AGTGGCAGTT CCAGAAGCGG CTGCAGGAGT CCAAGCAGAA GGACGAGGAC 
GACGAGGAGG AGGAGGACGA TGACGTGGAC ACCGTGCTGC TCATGCAGCG CCTGGAGGCC 
GAGCGCAGAG CGCGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGCCTGGG CCGCCCGCCA 
CTCCCGCGCG GCTATGAGCC CGCCGGCCCG CCGCCACCGC CCCAGCGCAC CGCCTCCTAC 
CTGCAGACGC AGGCCTTCTC CCCGGACGCC CTCTACACCG CCAAGTTCGT GGCCTACAAT 
GAGGAGGAGG ACGAGGACGA AGAGCCGGCA GGAGCAAACT CTTACGTGGG ACCTGCTGGG 
ACAGTTTTTG GAGCCCATGA TGTTTATCGG GACCCGAGAG AGAGACCGCC TAAGAGCCAC 
GAAGCAGATG TCCCCGGGGG TCCCGGAGCC CCTGAAAACC TGACGTTCAG GGAGCGCCAG 
CGCCTTTTCT CCCAAGGCCA CGATGTCTCC AATAAAGTCA AGGCCTCTCG CAAGTTAACA 
GAGCTGGAGA ACGAGCTGAA CACGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464901.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320387.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X19, mRNA.

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CloneID	OBa94640
Clone ID Related Accession (Same CDS sequence)	XM_015464901.1
Accession Version	XM_015464901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGGATGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGACAC GGGAGGCGGA GGAGCGCGCG 
CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG GACGAGCGGG TCAGGCGCGA GGCCGAAGAC 
AAGAGGCGGC AGGAGGACGG CTACTACAGC CGCCTGGAGG CCGAGCGGCG CCGACAGCAT 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCGCCACTC 
CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG CCACCGCCCC AGCGCACCGC CTCCTACCTG 
CAGACGCAGG CCTTCTCCCC GGACGCCCTC TACACCGCCA AGTTCGTGGC CTACAATGAG 
GAGGAGGACG AGGACGAAGA GCCGGCAGGA GCAAACTCTT ACGTGGGACC TGCTGGGACA 
GTTTTTGGAG CCCATGATGT TTATCGGGAC CCGAGAGAGA GACCGCCTAA GAGCCACGAA 
GCAGATGTCC CCGGGGGTCC CGGAGCCCCT GAAAACCTGA CGTTCAGGGA GCGCCAGCGC 
CTTTTCTCCC AAGGCCACGA TGTCTCCAAT AAAGTCAAGG CCTCTCGCAA GTTAACAGAG 
CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320387.1
CDS	63..5429
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464901.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464901.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGGATGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGACAC GGGAGGCGGA GGAGCGCGCG 
CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG GACGAGCGGG TCAGGCGCGA GGCCGAAGAC 
AAGAGGCGGC AGGAGGACGG CTACTACAGC CGCCTGGAGG CCGAGCGGCG CCGACAGCAT 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCGCCACTC 
CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG CCACCGCCCC AGCGCACCGC CTCCTACCTG 
CAGACGCAGG CCTTCTCCCC GGACGCCCTC TACACCGCCA AGTTCGTGGC CTACAATGAG 
GAGGAGGACG AGGACGAAGA GCCGGCAGGA GCAAACTCTT ACGTGGGACC TGCTGGGACA 
GTTTTTGGAG CCCATGATGT TTATCGGGAC CCGAGAGAGA GACCGCCTAA GAGCCACGAA 
GCAGATGTCC CCGGGGGTCC CGGAGCCCCT GAAAACCTGA CGTTCAGGGA GCGCCAGCGC 
CTTTTCTCCC AAGGCCACGA TGTCTCCAAT AAAGTCAAGG CCTCTCGCAA GTTAACAGAG 
CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464902.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320388.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X20, mRNA.

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CloneID	OBa94641
Clone ID Related Accession (Same CDS sequence)	XM_015464902.1
Accession Version	XM_015464902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA ATCAGCCTCC CAGTCCTGGC GGGAAGAGTG CGTATGCCTC CGGAACAGCA 
GCCAAGATCA CATCTGTCTC CACGGGGAAC CTCTGCGCTG AGGAGCAGAC GCCTCCGCCC 
CGCCCCGAAG CCTACCCCAT CCCCACGCAG ACGTCCACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAAGT CCCAGGACCG CGTGGCGCCC CCGCAGAGCC AGTGGCTGGG TTTCGAGGAG 
AAGCCCCACG TGCTGGCAGA CGGAAACCAC TCCAGCGCTG CCGTCCAGCG TGTGACCCGC 
TCCCAGGAGG AGCTCCGCGA GGACGCCTAC CACCCCGAGC GGCAGCGGCC GGAGGCAGCC 
GCGGATCGCA GGTCGGATGG CAGCGACGCC TGGCCCACCC AGGGCTCATC ACTGGGTTCC 
GGCGCGTCCA GCCAGGAGCA CCTGGGGCCC CCCTCCAAGG CGGGCACCCC CGCCTCCACG 
CTCACCAAGA GCGGCCCCGG GCGCTGGAAG ACCCCGGCCG CCTCCCCGCC CATGCGGACC 
GACCCGGCCC TGCCGCCACC GCCGCCCCCC GGGTCCTACG CCGGGGAGGC TGACGCGGAC 
GCGGACTCGC CCCCGCCTCC CGCCGCCACC GCCGCCGGCC AGGCGCAGGC GGCCGCCGAG 
CGCAAGAAAC GAGAGGAGCA GCAGCGCTGG TACGAGAAGG AAAAAGCGCG CCTGGACGAG 
GAGCGCGAGC GCCGGCGCCG GGAGCAGGAG CGCCGGCTGG GCCAGCTGCG CGCGCCCGCG 
CCGCCCCCCG CGCCCGCCCG CGCGCCCGAC ACGGTGATCC GCGAGCTGCA GCCGCAGCAG 
CAGCCGCGTA CCATCGAGCG CAGGGACCTG CAGTACATCA CGGTCAGCAG GGAGGAGCTG 
GCCTCCGGAG ACAGCCTGTC CCCCGACCCC TGGAAGCGCG ATGCGCGCGA GAAGTTGGAG 
AAGCAGCAGC AGCTGCACAT CGTGGACCTG CTGAGCCGCG AGATCCAGGA GCTGCAGGGC 
AAGGCCGAGC GCTCGGCCGA GGAGAGCGAC CGGCTGCGCA AGCTGCTGCT GGAGTGGCAG 
TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG AAGGACGAGG ACGACGAGGA GGAGGAGGAC 
GATGACGTGG ACACCGTGCT GCTCATGCAG CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG 
ACTGCTATGC CAGCAATCTC TGTTCTAGAT TTGTTGCAGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGAG GCGGCAGGAG 
GACGGCTACT ACAGCCGCCT GGAGGCCGAG CGGCGCCGAC AGCATGAGGA GGCCGAGCGC 
CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG GGCCGCCCGC CACTCCCGCG CGGCTATGAG 
CCCGCCGGCC CGCCGCCACC GCCCCAGCGC ACCGCCTCCT ACCTGCAGAC GCAGGCCTTC 
TCCCCGGACG CCCTCTACAC CGCCAAGTTC GTGGCCTACA ATGAGGAGGA GGACGAGGAC 
GAAGAGCCGG CAGGAGCAAA CTCTTACGTG GGACCTGCTG GGACAGTTTT TGGAGCCCAT 
GATGTTTATC GGGACCCGAG AGAGAGACCG CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG 
GGTCCCGGAG CCCCTGAAAA CCTGACGTTC AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC 
CACGATGTCT CCAATAAAGT CAAGGCCTCT CGCAAGTTAA CAGAGCTGGA GAACGAGCTG 
AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320388.1
CDS	63..5414
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464902.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464902.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGAAACA GAAGAACATT GCAGGGGCCC TTGCCTTCTG GATGGCCAAC 
GCGTCCGAGC TGCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGGAT CACACTGGAT 
GCACAGGACG TCCTGGCACA CTTGGTGCAG ATGGCCTTTA AGTACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAATAATTA CATGCCCGCT TTTCTGGACG ACCCCGAAGA GGACAGTCTG 
CAGAGACCAA AAATAGACGA CGTGCTGCAC ACGCTCACGG GCGCCATGTC CCTGCTGCGT 
CGCTGCCGGG TCAATGCCGC GCTGACCATC CAGCTCTTCT CCCAACTCTT CCACTTCATC 
AACATGTGGC TGTTCAACCG GCTGGTGACC GACCCCGAGT CGGGGCTCTG CTCCCACTAC 
TGGGGTGCCA TCCTCCGCCA GCAGCTCGGG CACGTGGAGG CCTGGGCCGA GAAGCAGGGC 
CTGGAGCTGG CGGCTGACTG CCACCTGAGC CGGATAGTGC AGGCAACCAC TTTGCTTACG 
ATGGATAAGT ACGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT CAAGTTAAAT 
TCACTGCAGC TCCAAGCCTT ACTGCAGAAT TACCACTGTG CACCCGATGA GCCTTTTGTC 
CCTGCGGACC TGATCGCGAA CGTGGTGGCC GTGGCCGAGA ACACAGCAGA CGAGCTGGCA 
CGCAGCGACG GGCGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCTTTCCTC 
CTGCCTGAGG ACGGCTACTC CTGTGACGTC GTCAGGAATG TCCCCAGCGG CCTGCAGGAG 
TTCCTGGACC CTCTCTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCACCC ACGCTCTCCA 
GGCACCTGGA CGATATATTT TGAAGGTGCA GATTACGAGA GTCACCTTTT GCGGGAGAAC 
ACAGAACTGG CACAGCCTCT GCGTAAAGAA CCTGAAATCA TCACTGTGAC CCTCAAAAAG 
CAGAACGGAA TGGGCCTTAG CATTGTTGCT GCAAAGGGCG CTGGTCAAGA CAAACTTGGG 
ATTTACGTCA AGTCCGTTGT GAAAGGAGGC GCTGCGGACG TGGACGGGCG GCTCGCCGCG 
GGTGACCAGC TGCTCAGCGT GGACGGACGG AGTCTGGTGG GACTCTCTCA AGAAAGGGCA 
GCAGAACTGA TGACAAGGAC AAGCTCCGTC GTGACGCTTG AAGTAGCAAA GCAAGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCTTCCC CTATGATGCA GAGAATTTCA 
GAGCGCCGTG GGTCAGGTAA ACCCCGACCA AAAAGCGAAG GCTTTGAGCT GTACAACAAC 
TCGGCTCAGA ACGGGTCCCC GGAGAGCCCG CAGACGCCCT GGGCCGAGTA CAGCGAGCCC 
AAGAAGCTGC CGGGCGATGA CCGGCTGACG AAGCACAGGG CAGACCACCG CTCCAGCCCC 
AACGTCGCAA ATCAGCCTCC CAGTCCTGGC GGGAAGAGTG CGTATGCCTC CGGAACAGCA 
GCCAAGATCA CATCTGTCTC CACGGGGAAC CTCTGCGCTG AGGAGCAGAC GCCTCCGCCC 
CGCCCCGAAG CCTACCCCAT CCCCACGCAG ACGTCCACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAAGT CCCAGGACCG CGTGGCGCCC CCGCAGAGCC AGTGGCTGGG TTTCGAGGAG 
AAGCCCCACG TGCTGGCAGA CGGAAACCAC TCCAGCGCTG CCGTCCAGCG TGTGACCCGC 
TCCCAGGAGG AGCTCCGCGA GGACGCCTAC CACCCCGAGC GGCAGCGGCC GGAGGCAGCC 
GCGGATCGCA GGTCGGATGG CAGCGACGCC TGGCCCACCC AGGGCTCATC ACTGGGTTCC 
GGCGCGTCCA GCCAGGAGCA CCTGGGGCCC CCCTCCAAGG CGGGCACCCC CGCCTCCACG 
CTCACCAAGA GCGGCCCCGG GCGCTGGAAG ACCCCGGCCG CCTCCCCGCC CATGCGGACC 
GACCCGGCCC TGCCGCCACC GCCGCCCCCC GGGTCCTACG CCGGGGAGGC TGACGCGGAC 
GCGGACTCGC CCCCGCCTCC CGCCGCCACC GCCGCCGGCC AGGCGCAGGC GGCCGCCGAG 
CGCAAGAAAC GAGAGGAGCA GCAGCGCTGG TACGAGAAGG AAAAAGCGCG CCTGGACGAG 
GAGCGCGAGC GCCGGCGCCG GGAGCAGGAG CGCCGGCTGG GCCAGCTGCG CGCGCCCGCG 
CCGCCCCCCG CGCCCGCCCG CGCGCCCGAC ACGGTGATCC GCGAGCTGCA GCCGCAGCAG 
CAGCCGCGTA CCATCGAGCG CAGGGACCTG CAGTACATCA CGGTCAGCAG GGAGGAGCTG 
GCCTCCGGAG ACAGCCTGTC CCCCGACCCC TGGAAGCGCG ATGCGCGCGA GAAGTTGGAG 
AAGCAGCAGC AGCTGCACAT CGTGGACCTG CTGAGCCGCG AGATCCAGGA GCTGCAGGGC 
AAGGCCGAGC GCTCGGCCGA GGAGAGCGAC CGGCTGCGCA AGCTGCTGCT GGAGTGGCAG 
TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG AAGGACGAGG ACGACGAGGA GGAGGAGGAC 
GATGACGTGG ACACCGTGCT GCTCATGCAG CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG 
ACTGCTATGC CAGCAATCTC TGTTCTAGAT TTGTTGCAGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGAG GCGGCAGGAG 
GACGGCTACT ACAGCCGCCT GGAGGCCGAG CGGCGCCGAC AGCATGAGGA GGCCGAGCGC 
CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG GGCCGCCCGC CACTCCCGCG CGGCTATGAG 
CCCGCCGGCC CGCCGCCACC GCCCCAGCGC ACCGCCTCCT ACCTGCAGAC GCAGGCCTTC 
TCCCCGGACG CCCTCTACAC CGCCAAGTTC GTGGCCTACA ATGAGGAGGA GGACGAGGAC 
GAAGAGCCGG CAGGAGCAAA CTCTTACGTG GGACCTGCTG GGACAGTTTT TGGAGCCCAT 
GATGTTTATC GGGACCCGAG AGAGAGACCG CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG 
GGTCCCGGAG CCCCTGAAAA CCTGACGTTC AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC 
CACGATGTCT CCAATAAAGT CAAGGCCTCT CGCAAGTTAA CAGAGCTGGA GAACGAGCTG 
AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464903.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320389.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X21, mRNA.

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CloneID	OBa94642
Clone ID Related Accession (Same CDS sequence)	XM_015464903.1 , XM_024997146.1
Accession Version	XM_015464903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320389.1
CDS	1..5346
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464903.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464903.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997146.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852914.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OBa94642
Clone ID Related Accession (Same CDS sequence)	XM_015464903.1 , XM_024997146.1
Accession Version	XM_024997146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852914.1
CDS	71..5416
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997146.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997146.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGATGAGG AGCGGCGGCG GCAGCAGCAG 
CTGGAGGAGA TGCGGACACG GGAGGCGGAG GAGCGCGCGC GGCAGGAGGA GCAGCGGCGC 
CGGCAGGAGG ACGAGCGGGT CAGGCGCGAG GCCGAAGACA AGAGGCGGCA GGAGGACGGC 
TACTACAGCC GCCTGGAGGC CGAGCGGCGC CGACAGCATG AGGAGGCCGA GCGCCGGCTG 
CTGGAGCCCG AGGAGCCGGG CCTGGGCCGC CCGCCACTCC CGCGCGGCTA TGAGCCCGCC 
GGCCCGCCGC CACCGCCCCA GCGCACCGCC TCCTACCTGC AGACGCAGGC CTTCTCCCCG 
GACGCCCTCT ACACCGCCAA GTTCGTGGCC TACAATGAGG AGGAGGACGA GGACGAAGAG 
CCGGCAGGAG CAAACTCTTA CGTGGGACCT GCTGGGACAG TTTTTGGAGC CCATGATGTT 
TATCGGGACC CGAGAGAGAG ACCGCCTAAG AGCCACGAAG CAGATGTCCC CGGGGGTCCC 
GGAGCCCCTG AAAACCTGAC GTTCAGGGAG CGCCAGCGCC TTTTCTCCCA AGGCCACGAT 
GTCTCCAATA AAGTCAAGGC CTCTCGCAAG TTAACAGAGC TGGAGAACGA GCTGAACACG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464904.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320390.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X22, mRNA.

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CloneID	OBa94643
Clone ID Related Accession (Same CDS sequence)	XM_015464904.1 , XM_024997147.1
Accession Version	XM_015464904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGTT GCAGGATGAG GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG 
ATGCGGACAC GGGAGGCGGA GGAGCGCGCG CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG 
GACGAGCGGG TCAGGCGCGA GGCCGAAGAC AAGAGGCGGC AGGAGGACGG CTACTACAGC 
CGCCTGGAGG CCGAGCGGCG CCGACAGCAT GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC 
GAGGAGCCGG GCCTGGGCCG CCCGCCACTC CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG 
CCACCGCCCC AGCGCACCGC CTCCTACCTG CAGACGCAGG CCTTCTCCCC GGACGCCCTC 
TACACCGCCA AGTTCGTGGC CTACAATGAG GAGGAGGACG AGGACGAAGA GCCGGCAGGA 
GCAAACTCTT ACGTGGGACC TGCTGGGACA GTTTTTGGAG CCCATGATGT TTATCGGGAC 
CCGAGAGAGA GACCGCCTAA GAGCCACGAA GCAGATGTCC CCGGGGGTCC CGGAGCCCCT 
GAAAACCTGA CGTTCAGGGA GCGCCAGCGC CTTTTCTCCC AAGGCCACGA TGTCTCCAAT 
AAAGTCAAGG CCTCTCGCAA GTTAACAGAG CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320390.1
CDS	63..5399
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464904.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464904.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGTT GCAGGATGAG GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG 
ATGCGGACAC GGGAGGCGGA GGAGCGCGCG CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG 
GACGAGCGGG TCAGGCGCGA GGCCGAAGAC AAGAGGCGGC AGGAGGACGG CTACTACAGC 
CGCCTGGAGG CCGAGCGGCG CCGACAGCAT GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC 
GAGGAGCCGG GCCTGGGCCG CCCGCCACTC CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG 
CCACCGCCCC AGCGCACCGC CTCCTACCTG CAGACGCAGG CCTTCTCCCC GGACGCCCTC 
TACACCGCCA AGTTCGTGGC CTACAATGAG GAGGAGGACG AGGACGAAGA GCCGGCAGGA 
GCAAACTCTT ACGTGGGACC TGCTGGGACA GTTTTTGGAG CCCATGATGT TTATCGGGAC 
CCGAGAGAGA GACCGCCTAA GAGCCACGAA GCAGATGTCC CCGGGGGTCC CGGAGCCCCT 
GAAAACCTGA CGTTCAGGGA GCGCCAGCGC CTTTTCTCCC AAGGCCACGA TGTCTCCAAT 
AAAGTCAAGG CCTCTCGCAA GTTAACAGAG CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997147.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852915.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OBa94643
Clone ID Related Accession (Same CDS sequence)	XM_015464904.1 , XM_024997147.1
Accession Version	XM_024997147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGTT GCAGGATGAG GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG 
ATGCGGACAC GGGAGGCGGA GGAGCGCGCG CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG 
GACGAGCGGG TCAGGCGCGA GGCCGAAGAC AAGAGGCGGC AGGAGGACGG CTACTACAGC 
CGCCTGGAGG CCGAGCGGCG CCGACAGCAT GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC 
GAGGAGCCGG GCCTGGGCCG CCCGCCACTC CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG 
CCACCGCCCC AGCGCACCGC CTCCTACCTG CAGACGCAGG CCTTCTCCCC GGACGCCCTC 
TACACCGCCA AGTTCGTGGC CTACAATGAG GAGGAGGACG AGGACGAAGA GCCGGCAGGA 
GCAAACTCTT ACGTGGGACC TGCTGGGACA GTTTTTGGAG CCCATGATGT TTATCGGGAC 
CCGAGAGAGA GACCGCCTAA GAGCCACGAA GCAGATGTCC CCGGGGGTCC CGGAGCCCCT 
GAAAACCTGA CGTTCAGGGA GCGCCAGCGC CTTTTCTCCC AAGGCCACGA TGTCTCCAAT 
AAAGTCAAGG CCTCTCGCAA GTTAACAGAG CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852915.1
CDS	70..5406
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997147.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997147.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGTT GCAGGATGAG GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG 
ATGCGGACAC GGGAGGCGGA GGAGCGCGCG CGGCAGGAGG AGCAGCGGCG CCGGCAGGAG 
GACGAGCGGG TCAGGCGCGA GGCCGAAGAC AAGAGGCGGC AGGAGGACGG CTACTACAGC 
CGCCTGGAGG CCGAGCGGCG CCGACAGCAT GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC 
GAGGAGCCGG GCCTGGGCCG CCCGCCACTC CCGCGCGGCT ATGAGCCCGC CGGCCCGCCG 
CCACCGCCCC AGCGCACCGC CTCCTACCTG CAGACGCAGG CCTTCTCCCC GGACGCCCTC 
TACACCGCCA AGTTCGTGGC CTACAATGAG GAGGAGGACG AGGACGAAGA GCCGGCAGGA 
GCAAACTCTT ACGTGGGACC TGCTGGGACA GTTTTTGGAG CCCATGATGT TTATCGGGAC 
CCGAGAGAGA GACCGCCTAA GAGCCACGAA GCAGATGTCC CCGGGGGTCC CGGAGCCCCT 
GAAAACCTGA CGTTCAGGGA GCGCCAGCGC CTTTTCTCCC AAGGCCACGA TGTCTCCAAT 
AAAGTCAAGG CCTCTCGCAA GTTAACAGAG CTGGAGAACG AGCTGAACAC GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464905.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320391.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X23, mRNA.

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CloneID	OBa94644
Clone ID Related Accession (Same CDS sequence)	XM_015464905.1
Accession Version	XM_015464905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGGA TGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
ACACGGGAGG CGGAGGAGCG CGCGCGGCAG GAGGAGCAGC GGCGCCGGCA GGAGGACGAG 
CGGGTCAGGC GCGAGGCCGA AGACAAGAGG CGGCAGGAGG ACGGCTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCCGACA GCATGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC GGCTATGAGC CCGCCGGCCC GCCGCCACCG 
CCCCAGCGCA CCGCCTCCTA CCTGCAGACG CAGGCCTTCT CCCCGGACGC CCTCTACACC 
GCCAAGTTCG TGGCCTACAA TGAGGAGGAG GACGAGGACG AAGAGCCGGC AGGAGCAAAC 
TCTTACGTGG GACCTGCTGG GACAGTTTTT GGAGCCCATG ATGTTTATCG GGACCCGAGA 
GAGAGACCGC CTAAGAGCCA CGAAGCAGAT GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC 
CTGACGTTCA GGGAGCGCCA GCGCCTTTTC TCCCAAGGCC ACGATGTCTC CAATAAAGTC 
AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320391.1
CDS	63..5393
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464905.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464905.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGGA TGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
ACACGGGAGG CGGAGGAGCG CGCGCGGCAG GAGGAGCAGC GGCGCCGGCA GGAGGACGAG 
CGGGTCAGGC GCGAGGCCGA AGACAAGAGG CGGCAGGAGG ACGGCTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCCGACA GCATGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCGCC ACTCCCGCGC GGCTATGAGC CCGCCGGCCC GCCGCCACCG 
CCCCAGCGCA CCGCCTCCTA CCTGCAGACG CAGGCCTTCT CCCCGGACGC CCTCTACACC 
GCCAAGTTCG TGGCCTACAA TGAGGAGGAG GACGAGGACG AAGAGCCGGC AGGAGCAAAC 
TCTTACGTGG GACCTGCTGG GACAGTTTTT GGAGCCCATG ATGTTTATCG GGACCCGAGA 
GAGAGACCGC CTAAGAGCCA CGAAGCAGAT GTCCCCGGGG GTCCCGGAGC CCCTGAAAAC 
CTGACGTTCA GGGAGCGCCA GCGCCTTTTC TCCCAAGGCC ACGATGTCTC CAATAAAGTC 
AAGGCCTCTC GCAAGTTAAC AGAGCTGGAG AACGAGCTGA ACACGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464906.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320392.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X24, mRNA.

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CloneID	OBa94645
Clone ID Related Accession (Same CDS sequence)	XM_015464906.1
Accession Version	XM_015464906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGCTTCCT 
GCCAGGCTGT TTTGGTGGCG TGCGACTTCT CCATCCTTCA GGGTAAAACA GCAAGCTCGT 
CTTCTCTCCA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320392.1
CDS	64..4878
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARQTAMPAISVLDLLPARLFWWRATSPSFRVKQQARLLSI

Target ORF information:

RefSeq Version	XM_015464906.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464906.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCCCTGGTGG TGCAGCTGAA CTGGAATAAA 
GATGACCGAG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ATGCTGTTCC TGCAAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TCATCCAGAA CTTTAAGAGA 
ACTCTCTCAA AGAAGGAGAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC AGTGAGACAG 
GCGTCTGAGA AAGATGACAG ACCTTTCTCT GGGGATGACA CTGAGAATTC TCGCCTGGCC 
GCTGAGGTCT ACAAAGACAT GCCTGAGACC AGCTTTACGC GAACCATCTC CAACCCTGAG 
GTGGTCATGA AGCGGCGGCG GCAGCAGAAG CTGGAGAGGA GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCAGACTC GGGTGGAACA TTGAGAATTT ACGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACGAT CCTGCTGTCC ACTACAGACC CTGCGGACTT TGCTGTGGCC 
GAAGCCTTGG AGAAGTACGG CCTGGAGAAG GAGAACCCCA GGGACTACTG CATCGCCCGT 
GTCATGCTTC CTCCTGGAGC CCAGCATTCG GATGAAAAAG GTGCTAAAGA AGTTATTCTT 
GATGATGACG AATGTCCTTT ACAGATCTTC AGGGAATGGC CGAGTGACAG AGGGATTTTA 
GTCTTTCAGT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAAGTAA GAAGCACACG 
GACGGGAAGC CCCTGCAGGG GAAGGAGAGA GCCGATGGGT CGGCCTACGG CTCCGCCCTG 
CCCCCCGAGA AGCTGCCCTA CCTCGTGGAG CTGAGCCCGG ACGGTTCTGA CTCCAGAGAC 
AAGCCAAAGC TCTACCGCCT TCAGCTCAGC GTCACGGAGG TGGGGACCGA GAAGTTCGAC 
GACAGCTCCA TCCAGTTGTT TGGTTCAGGC ATTCAGCCCC ATCACTGTGA CCTCACGAAT 
ATGGACGGGG TGGTCACGGT CACGCCGAGA AGCATGGACG CTGAGACCTA CGTGGATGGG 
CAGCGCATCT CGGAGACCAC GATGCTGCAG AGCGGGATGA AGGTGCAGTT CGGGGCGTCC 
CACGTGTTCA AGTTCGTGGA CCCCAGCCAG GACCACACGC TCCCCAAGAG ATCCGTGGAC 
GGGGGCCTGA CTGTGAAGGG CCCCAGACAT AAGTCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTAGGAG GAGACGTTCA CAGTGGAATG GCCTTACCAG CGAGCAAGAG CACCGCCAGG 
CTGGACAGCG ACAGGACGCC GTCCACCTCC AGCACCGCCG AGCGGGGGAT GGTGAAGCCG 
ATGGTCCGCG TGGAGCAGCA GCTGGACTAC CGCCGGCAGG ACAGCAGAAC TCAGGATGCT 
CCCGGGCCTG ACCTGATTCT GCCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCC 
TTTTTATCTG CCATTATAAA TTACACTAAT AGCTCTACAG TCCATTTTAA GTTGTCGCCC 
ACTTACGTGT TATACATGGC CTGTCGGTAT GTATTGTCCA GCCAGTACAG ACCCGACGTC 
AGCCCCGCGG AACGCACGCA CAAAGTCATG GCCATCGTCA ACAAGATGGT GAGCATGATG 
GAAGGGGTGA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGGGCC 
CTTGCCTTCT GGATGGCCAA CGCGTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTCAGTCGGA TCACACTGGA TGCACAGGAC GTCCTGGCAC ACTTGGTGCA GATGGCCTTT 
AAGTACCTGG TTCACTGTCT TCAGTCAGAG CTGAATAATT ACATGCCCGC TTTTCTGGAC 
GACCCCGAAG AGGACAGTCT GCAGAGACCA AAAATAGACG ACGTGCTGCA CACGCTCACG 
GGCGCCATGT CCCTGCTGCG TCGCTGCCGG GTCAATGCCG CGCTGACCAT CCAGCTCTTC 
TCCCAACTCT TCCACTTCAT CAACATGTGG CTGTTCAACC GGCTGGTGAC CGACCCCGAG 
TCGGGGCTCT GCTCCCACTA CTGGGGTGCC ATCCTCCGCC AGCAGCTCGG GCACGTGGAG 
GCCTGGGCCG AGAAGCAGGG CCTGGAGCTG GCGGCTGACT GCCACCTGAG CCGGATAGTG 
CAGGCAACCA CTTTGCTTAC GATGGATAAG TACGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TCAAGTTAAA TTCACTGCAG CTCCAAGCCT TACTGCAGAA TTACCACTGT 
GCACCCGATG AGCCTTTTGT CCCTGCGGAC CTGATCGCGA ACGTGGTGGC CGTGGCCGAG 
AACACAGCAG ACGAGCTGGC ACGCAGCGAC GGGCGGGAGG TGCGGCTGGA GGAGGACCCT 
GACCTGCAGC TGCCTTTCCT CCTGCCTGAG GACGGCTACT CCTGTGACGT CGTCAGGAAT 
GTCCCCAGCG GCCTGCAGGA GTTCCTGGAC CCTCTCTGCC AGAGAGGGTT TTGCAGGTTA 
ATTCCTCACC CACGCTCTCC AGGCACCTGG ACGATATATT TTGAAGGTGC AGATTACGAG 
AGTCACCTTT TGCGGGAGAA CACAGAACTG GCACAGCCTC TGCGTAAAGA ACCTGAAATC 
ATCACTGTGA CCCTCAAAAA GCAGAACGGA ATGGGCCTTA GCATTGTTGC TGCAAAGGGC 
GCTGGTCAAG ACAAACTTGG GATTTACGTC AAGTCCGTTG TGAAAGGAGG CGCTGCGGAC 
GTGGACGGGC GGCTCGCCGC GGGTGACCAG CTGCTCAGCG TGGACGGACG GAGTCTGGTG 
GGACTCTCTC AAGAAAGGGC AGCAGAACTG ATGACAAGGA CAAGCTCCGT CGTGACGCTT 
GAAGTAGCAA AGCAAGGAGC CATTTACCAC GGCCTCGCCA CCCTGCTCAA CCAGCCTTCC 
CCTATGATGC AGAGAATTTC AGAGCGCCGT GGGTCAGGTA AACCCCGACC AAAAAGCGAA 
GGCTTTGAGC TGTACAACAA CTCGGCTCAG AACGGGTCCC CGGAGAGCCC GCAGACGCCC 
TGGGCCGAGT ACAGCGAGCC CAAGAAGCTG CCGGGCGATG ACCGGCTGAC GAAGCACAGG 
GCAGACCACC GCTCCAGCCC CAACGTCGCA AATCAGCCTC CCAGTCCTGG CGGGAAGAGT 
GCGTATGCCT CCGGAACAGC AGCCAAGATC ACATCTGTCT CCACGGGGAA CCTCTGCGCT 
GAGGAGCAGA CGCCTCCGCC CCGCCCCGAA GCCTACCCCA TCCCCACGCA GACGTCCACC 
AGGGAGTACT TCACCTTCCC GGCCTCCAAG TCCCAGGACC GCGTGGCGCC CCCGCAGAGC 
CAGTGGCTGG GTTTCGAGGA GAAGCCCCAC GTGCTGGCAG ACGGAAACCA CTCCAGCGCT 
GCCGTCCAGC GTGTGACCCG CTCCCAGGAG GAGCTCCGCG AGGACGCCTA CCACCCCGAG 
CGGCAGCGGC CGGAGGCAGC CGCGGATCGC AGGTCGGATG GCAGCGACGC CTGGCCCACC 
CAGGGCTCAT CACTGGGTTC CGGCGCGTCC AGCCAGGAGC ACCTGGGGCC CCCCTCCAAG 
GCGGGCACCC CCGCCTCCAC GCTCACCAAG AGCGGCCCCG GGCGCTGGAA GACCCCGGCC 
GCCTCCCCGC CCATGCGGAC CGACCCGGCC CTGCCGCCAC CGCCGCCCCC CGGGTCCTAC 
GCCGGGGAGG CTGACGCGGA CGCGGACTCG CCCCCGCCTC CCGCCGCCAC CGCCGCCGGC 
CAGGCGCAGG CGGCCGCCGA GCGCAAGAAA CGAGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAAAAAGCGC GCCTGGACGA GGAGCGCGAG CGCCGGCGCC GGGAGCAGGA GCGCCGGCTG 
GGCCAGCTGC GCGCGCCCGC GCCGCCCCCC GCGCCCGCCC GCGCGCCCGA CACGGTGATC 
CGCGAGCTGC AGCCGCAGCA GCAGCCGCGT ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACGGTCAGCA GGGAGGAGCT GGCCTCCGGA GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GATGCGCGCG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTGGACCT GCTGAGCCGC 
GAGATCCAGG AGCTGCAGGG CAAGGCCGAG CGCTCGGCCG AGGAGAGCGA CCGGCTGCGC 
AAGCTGCTGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGATGACGTG GACACCGTGC TGCTCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGCTTCCT 
GCCAGGCTGT TTTGGTGGCG TGCGACTTCT CCATCCTTCA GGGTAAAACA GCAAGCTCGT 
CTTCTCTCCA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464907.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320393.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X25, mRNA.

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CloneID	OBa94646
Clone ID Related Accession (Same CDS sequence)	XM_015464907.1
Accession Version	XM_015464907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGGGA GAAGGAACCA CTCGGCCTGC 
TACAGCTCTC ACGCTTCCGA AGACGGTTCT GACTCCAGAG ACAAGCCAAA GCTCTACCGC 
CTTCAGCTCA GCGTCACGGA GGTGGGGACC GAGAAGTTCG ACGACAGCTC CATCCAGTTG 
TTTGGTTCAG GCATTCAGCC CCATCACTGT GACCTCACGA ATATGGACGG GGTGGTCACG 
GTCACGCCGA GAAGCATGGA CGCTGAGACC TACGTGGATG GGCAGCGCAT CTCGGAGACC 
ACGATGCTGC AGAGCGGGAT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTCGTG 
GACCCCAGCC AGGACCACAC GCTCCCCAAG AGATCCGTGG ACGGGGGCCT GACTGTGAAG 
GGCCCCAGAC ATAAGTCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGACGTT 
CACAGTGGAA TGGCCTTACC AGCGAGCAAG AGCACCGCCA GGCTGGACAG CGACAGGACG 
CCGTCCACCT CCAGCACCGC CGAGCGGGGG ATGGTGAAGC CGATGGTCCG CGTGGAGCAG 
CAGCTGGACT ACCGCCGGCA GGACAGCAGA ACTCAGGATG CTCCCGGGCC TGACCTGATT 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CCTTTTTATC TGCCATTATA 
AATTACACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCGC CCACTTACGT GTTATACATG 
GCCTGTCGGT ATGTATTGTC CAGCCAGTAC AGACCCGACG TCAGCCCCGC GGAACGCACG 
CACAAAGTCA TGGCCATCGT CAACAAGATG GTGAGCATGA TGGAAGGGGT GATCCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGT TGCAGGATGA GGAGCGGCGG 
CGGCAGCAGC AGCTGGAGGA GATGCGGACA CGGGAGGCGG AGGAGCGCGC GCGGCAGGAG 
GAGCAGCGGC GCCGGCAGGA GGACGAGCGG GTCAGGCGCG AGGCCGAAGA CAAGAGGCGG 
CAGGAGGACG GCTACTACAG CCGCCTGGAG GCCGAGCGGC GCCGACAGCA TGAGGAGGCC 
GAGCGCCGGC TGCTGGAGCC CGAGGAGCCG GGCCTGGGCC GCCCGCCACT CCCGCGCGGC 
TATGAGCCCG CCGGCCCGCC GCCACCGCCC CAGCGCACCG CCTCCTACCT GCAGACGCAG 
GCCTTCTCCC CGGACGCCCT CTACACCGCC AAGTTCGTGG CCTACAATGA GGAGGAGGAC 
GAGGACGAAG AGCCGGCAGG AGCAAACTCT TACGTGGGAC CTGCTGGGAC AGTTTTTGGA 
GCCCATGATG TTTATCGGGA CCCGAGAGAG AGACCGCCTA AGAGCCACGA AGCAGATGTC 
CCCGGGGGTC CCGGAGCCCC TGAAAACCTG ACGTTCAGGG AGCGCCAGCG CCTTTTCTCC 
CAAGGCCACG ATGTCTCCAA TAAAGTCAAG GCCTCTCGCA AGTTAACAGA GCTGGAGAAC 
GAGCTGAACA CGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320393.1
CDS	65..5422
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPAGPPPPPQRTASYLQTQAFSPDALYTAKFVAYNEEEDEDEEPAGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464907.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464907.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGGGA GAAGGAACCA CTCGGCCTGC 
TACAGCTCTC ACGCTTCCGA AGACGGTTCT GACTCCAGAG ACAAGCCAAA GCTCTACCGC 
CTTCAGCTCA GCGTCACGGA GGTGGGGACC GAGAAGTTCG ACGACAGCTC CATCCAGTTG 
TTTGGTTCAG GCATTCAGCC CCATCACTGT GACCTCACGA ATATGGACGG GGTGGTCACG 
GTCACGCCGA GAAGCATGGA CGCTGAGACC TACGTGGATG GGCAGCGCAT CTCGGAGACC 
ACGATGCTGC AGAGCGGGAT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTCGTG 
GACCCCAGCC AGGACCACAC GCTCCCCAAG AGATCCGTGG ACGGGGGCCT GACTGTGAAG 
GGCCCCAGAC ATAAGTCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGACGTT 
CACAGTGGAA TGGCCTTACC AGCGAGCAAG AGCACCGCCA GGCTGGACAG CGACAGGACG 
CCGTCCACCT CCAGCACCGC CGAGCGGGGG ATGGTGAAGC CGATGGTCCG CGTGGAGCAG 
CAGCTGGACT ACCGCCGGCA GGACAGCAGA ACTCAGGATG CTCCCGGGCC TGACCTGATT 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CCTTTTTATC TGCCATTATA 
AATTACACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCGC CCACTTACGT GTTATACATG 
GCCTGTCGGT ATGTATTGTC CAGCCAGTAC AGACCCGACG TCAGCCCCGC GGAACGCACG 
CACAAAGTCA TGGCCATCGT CAACAAGATG GTGAGCATGA TGGAAGGGGT GATCCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGT TGCAGGATGA GGAGCGGCGG 
CGGCAGCAGC AGCTGGAGGA GATGCGGACA CGGGAGGCGG AGGAGCGCGC GCGGCAGGAG 
GAGCAGCGGC GCCGGCAGGA GGACGAGCGG GTCAGGCGCG AGGCCGAAGA CAAGAGGCGG 
CAGGAGGACG GCTACTACAG CCGCCTGGAG GCCGAGCGGC GCCGACAGCA TGAGGAGGCC 
GAGCGCCGGC TGCTGGAGCC CGAGGAGCCG GGCCTGGGCC GCCCGCCACT CCCGCGCGGC 
TATGAGCCCG CCGGCCCGCC GCCACCGCCC CAGCGCACCG CCTCCTACCT GCAGACGCAG 
GCCTTCTCCC CGGACGCCCT CTACACCGCC AAGTTCGTGG CCTACAATGA GGAGGAGGAC 
GAGGACGAAG AGCCGGCAGG AGCAAACTCT TACGTGGGAC CTGCTGGGAC AGTTTTTGGA 
GCCCATGATG TTTATCGGGA CCCGAGAGAG AGACCGCCTA AGAGCCACGA AGCAGATGTC 
CCCGGGGGTC CCGGAGCCCC TGAAAACCTG ACGTTCAGGG AGCGCCAGCG CCTTTTCTCC 
CAAGGCCACG ATGTCTCCAA TAAAGTCAAG GCCTCTCGCA AGTTAACAGA GCTGGAGAAC 
GAGCTGAACA CGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852921.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

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CloneID	OBa94647
Clone ID Related Accession (Same CDS sequence)	XM_024997153.1 , XM_015464908.1
Accession Version	XM_024997153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGAGCAAA CTCTTACGTG 
GGACCTGCTG GGACAGTTTT TGGAGCCCAT GATGTTTATC GGGACCCGAG AGAGAGACCG 
CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG GGTCCCGGAG CCCCTGAAAA CCTGACGTTC 
AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC CACGATGTCT CCAATAAAGT CAAGGCCTCT 
CGCAAGTTAA CAGAGCTGGA GAACGAGCTG AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852921.1
CDS	71..5212
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024997153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997153.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGAGCAAA CTCTTACGTG 
GGACCTGCTG GGACAGTTTT TGGAGCCCAT GATGTTTATC GGGACCCGAG AGAGAGACCG 
CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG GGTCCCGGAG CCCCTGAAAA CCTGACGTTC 
AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC CACGATGTCT CCAATAAAGT CAAGGCCTCT 
CGCAAGTTAA CAGAGCTGGA GAACGAGCTG AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464908.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320394.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X26, mRNA.

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CloneID	OBa94647
Clone ID Related Accession (Same CDS sequence)	XM_024997153.1 , XM_015464908.1
Accession Version	XM_015464908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGAGCAAA CTCTTACGTG 
GGACCTGCTG GGACAGTTTT TGGAGCCCAT GATGTTTATC GGGACCCGAG AGAGAGACCG 
CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG GGTCCCGGAG CCCCTGAAAA CCTGACGTTC 
AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC CACGATGTCT CCAATAAAGT CAAGGCCTCT 
CGCAAGTTAA CAGAGCTGGA GAACGAGCTG AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320394.1
CDS	64..5205
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARLQDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKRRQEDGYYSRLEAERRRQHEEAERRLLEPEEPGANSYVGPAGTVFGAHDVYRDPRERPPKSHEADVPGGPGAPENLTFRERQRLFSQGHDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_015464908.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464908.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTCATCC AGAACTTTAA GAGAACTCTC 
TCAAAGAAGG AGAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCAGTGAG ACAGGCGTCT 
GAGAAAGATG ACAGACCTTT CTCTGGGGAT GACACTGAGA ATTCTCGCCT GGCCGCTGAG 
GTCTACAAAG ACATGCCTGA GACCAGCTTT ACGCGAACCA TCTCCAACCC TGAGGTGGTC 
ATGAAGCGGC GGCGGCAGCA GAAGCTGGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCAG ACTCGGGTGG AACATTGAGA ATTTACGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CGATCCTGCT GTCCACTACA GACCCTGCGG ACTTTGCTGT GGCCGAAGCC 
TTGGAGAAGT ACGGCCTGGA GAAGGAGAAC CCCAGGGACT ACTGCATCGC CCGTGTCATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAA AAAGGTGCTA AAGAAGTTAT TCTTGATGAT 
GACGAATGTC CTTTACAGAT CTTCAGGGAA TGGCCGAGTG ACAGAGGGAT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA GTAAGAAGCA CACGGACGGG 
AAGCCCCTGC AGGGGAAGGA GAGAGCCGAT GGGTCGGCCT ACGGCTCCGC CCTGCCCCCC 
GAGAAGCTGC CCTACCTCGT GGAGCTGAGC CCGGACGGTT CTGACTCCAG AGACAAGCCA 
AAGCTCTACC GCCTTCAGCT CAGCGTCACG GAGGTGGGGA CCGAGAAGTT CGACGACAGC 
TCCATCCAGT TGTTTGGTTC AGGCATTCAG CCCCATCACT GTGACCTCAC GAATATGGAC 
GGGGTGGTCA CGGTCACGCC GAGAAGCATG GACGCTGAGA CCTACGTGGA TGGGCAGCGC 
ATCTCGGAGA CCACGATGCT GCAGAGCGGG ATGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTCG TGGACCCCAG CCAGGACCAC ACGCTCCCCA AGAGATCCGT GGACGGGGGC 
CTGACTGTGA AGGGCCCCAG ACATAAGTCT GGAATTGTTC AGGAGACAAC TTTTGATTTA 
GGAGGAGACG TTCACAGTGG AATGGCCTTA CCAGCGAGCA AGAGCACCGC CAGGCTGGAC 
AGCGACAGGA CGCCGTCCAC CTCCAGCACC GCCGAGCGGG GGATGGTGAA GCCGATGGTC 
CGCGTGGAGC AGCAGCTGGA CTACCGCCGG CAGGACAGCA GAACTCAGGA TGCTCCCGGG 
CCTGACCTGA TTCTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCCTTTTTA 
TCTGCCATTA TAAATTACAC TAATAGCTCT ACAGTCCATT TTAAGTTGTC GCCCACTTAC 
GTGTTATACA TGGCCTGTCG GTATGTATTG TCCAGCCAGT ACAGACCCGA CGTCAGCCCC 
GCGGAACGCA CGCACAAAGT CATGGCCATC GTCAACAAGA TGGTGAGCAT GATGGAAGGG 
GTGATCCAGA AACAGAAGAA CATTGCAGGG GCCCTTGCCT TCTGGATGGC CAACGCGTCC 
GAGCTGCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GGATCACACT GGATGCACAG 
GACGTCCTGG CACACTTGGT GCAGATGGCC TTTAAGTACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAATA ATTACATGCC CGCTTTTCTG GACGACCCCG AAGAGGACAG TCTGCAGAGA 
CCAAAAATAG ACGACGTGCT GCACACGCTC ACGGGCGCCA TGTCCCTGCT GCGTCGCTGC 
CGGGTCAATG CCGCGCTGAC CATCCAGCTC TTCTCCCAAC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACCGGCTGGT GACCGACCCC GAGTCGGGGC TCTGCTCCCA CTACTGGGGT 
GCCATCCTCC GCCAGCAGCT CGGGCACGTG GAGGCCTGGG CCGAGAAGCA GGGCCTGGAG 
CTGGCGGCTG ACTGCCACCT GAGCCGGATA GTGCAGGCAA CCACTTTGCT TACGATGGAT 
AAGTACGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTCAAGTT AAATTCACTG 
CAGCTCCAAG CCTTACTGCA GAATTACCAC TGTGCACCCG ATGAGCCTTT TGTCCCTGCG 
GACCTGATCG CGAACGTGGT GGCCGTGGCC GAGAACACAG CAGACGAGCT GGCACGCAGC 
GACGGGCGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCTTT CCTCCTGCCT 
GAGGACGGCT ACTCCTGTGA CGTCGTCAGG AATGTCCCCA GCGGCCTGCA GGAGTTCCTG 
GACCCTCTCT GCCAGAGAGG GTTTTGCAGG TTAATTCCTC ACCCACGCTC TCCAGGCACC 
TGGACGATAT ATTTTGAAGG TGCAGATTAC GAGAGTCACC TTTTGCGGGA GAACACAGAA 
CTGGCACAGC CTCTGCGTAA AGAACCTGAA ATCATCACTG TGACCCTCAA AAAGCAGAAC 
GGAATGGGCC TTAGCATTGT TGCTGCAAAG GGCGCTGGTC AAGACAAACT TGGGATTTAC 
GTCAAGTCCG TTGTGAAAGG AGGCGCTGCG GACGTGGACG GGCGGCTCGC CGCGGGTGAC 
CAGCTGCTCA GCGTGGACGG ACGGAGTCTG GTGGGACTCT CTCAAGAAAG GGCAGCAGAA 
CTGATGACAA GGACAAGCTC CGTCGTGACG CTTGAAGTAG CAAAGCAAGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCT TCCCCTATGA TGCAGAGAAT TTCAGAGCGC 
CGTGGGTCAG GTAAACCCCG ACCAAAAAGC GAAGGCTTTG AGCTGTACAA CAACTCGGCT 
CAGAACGGGT CCCCGGAGAG CCCGCAGACG CCCTGGGCCG AGTACAGCGA GCCCAAGAAG 
CTGCCGGGCG ATGACCGGCT GACGAAGCAC AGGGCAGACC ACCGCTCCAG CCCCAACGTC 
GCAAATCAGC CTCCCAGTCC TGGCGGGAAG AGTGCGTATG CCTCCGGAAC AGCAGCCAAG 
ATCACATCTG TCTCCACGGG GAACCTCTGC GCTGAGGAGC AGACGCCTCC GCCCCGCCCC 
GAAGCCTACC CCATCCCCAC GCAGACGTCC ACCAGGGAGT ACTTCACCTT CCCGGCCTCC 
AAGTCCCAGG ACCGCGTGGC GCCCCCGCAG AGCCAGTGGC TGGGTTTCGA GGAGAAGCCC 
CACGTGCTGG CAGACGGAAA CCACTCCAGC GCTGCCGTCC AGCGTGTGAC CCGCTCCCAG 
GAGGAGCTCC GCGAGGACGC CTACCACCCC GAGCGGCAGC GGCCGGAGGC AGCCGCGGAT 
CGCAGGTCGG ATGGCAGCGA CGCCTGGCCC ACCCAGGGCT CATCACTGGG TTCCGGCGCG 
TCCAGCCAGG AGCACCTGGG GCCCCCCTCC AAGGCGGGCA CCCCCGCCTC CACGCTCACC 
AAGAGCGGCC CCGGGCGCTG GAAGACCCCG GCCGCCTCCC CGCCCATGCG GACCGACCCG 
GCCCTGCCGC CACCGCCGCC CCCCGGGTCC TACGCCGGGG AGGCTGACGC GGACGCGGAC 
TCGCCCCCGC CTCCCGCCGC CACCGCCGCC GGCCAGGCGC AGGCGGCCGC CGAGCGCAAG 
AAACGAGAGG AGCAGCAGCG CTGGTACGAG AAGGAAAAAG CGCGCCTGGA CGAGGAGCGC 
GAGCGCCGGC GCCGGGAGCA GGAGCGCCGG CTGGGCCAGC TGCGCGCGCC CGCGCCGCCC 
CCCGCGCCCG CCCGCGCGCC CGACACGGTG ATCCGCGAGC TGCAGCCGCA GCAGCAGCCG 
CGTACCATCG AGCGCAGGGA CCTGCAGTAC ATCACGGTCA GCAGGGAGGA GCTGGCCTCC 
GGAGACAGCC TGTCCCCCGA CCCCTGGAAG CGCGATGCGC GCGAGAAGTT GGAGAAGCAG 
CAGCAGCTGC ACATCGTGGA CCTGCTGAGC CGCGAGATCC AGGAGCTGCA GGGCAAGGCC 
GAGCGCTCGG CCGAGGAGAG CGACCGGCTG CGCAAGCTGC TGCTGGAGTG GCAGTTCCAG 
AAGCGGCTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGATGAC 
GTGGACACCG TGCTGCTCAT GCAGCGCCTG GAGGCCGAGC GCAGAGCGCG GTTGCAGGAT 
GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG GAGATGCGGA CACGGGAGGC GGAGGAGCGC 
GCGCGGCAGG AGGAGCAGCG GCGCCGGCAG GAGGACGAGC GGGTCAGGCG CGAGGCCGAA 
GACAAGAGGC GGCAGGAGGA CGGCTACTAC AGCCGCCTGG AGGCCGAGCG GCGCCGACAG 
CATGAGGAGG CCGAGCGCCG GCTGCTGGAG CCCGAGGAGC CGGGAGCAAA CTCTTACGTG 
GGACCTGCTG GGACAGTTTT TGGAGCCCAT GATGTTTATC GGGACCCGAG AGAGAGACCG 
CCTAAGAGCC ACGAAGCAGA TGTCCCCGGG GGTCCCGGAG CCCCTGAAAA CCTGACGTTC 
AGGGAGCGCC AGCGCCTTTT CTCCCAAGGC CACGATGTCT CCAATAAAGT CAAGGCCTCT 
CGCAAGTTAA CAGAGCTGGA GAACGAGCTG AACACGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_015464909.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320395.1 Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OBa94648
Clone ID Related Accession (Same CDS sequence)	XM_015464909.1 , XM_024997154.1
Accession Version	XM_015464909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	afadin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGGGA GAAGGAACCA CTCGGCCTGC 
TACAGCTCTC ACGCTTCCGA AGACGGTTCT GACTCCAGAG ACAAGCCAAA GCTCTACCGC 
CTTCAGCTCA GCGTCACGGA GGTGGGGACC GAGAAGTTCG ACGACAGCTC CATCCAGTTG 
TTTGGTTCAG GCATTCAGCC CCATCACTGT GACCTCACGA ATATGGACGG GGTGGTCACG 
GTCACGCCGA GAAGCATGGA CGCTGAGACC TACGTGGATG GGCAGCGCAT CTCGGAGACC 
ACGATGCTGC AGAGCGGGAT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTCGTG 
GACCCCAGCC AGGACCACAC GCTCCCCAAG AGATCCGTGG ACGGGGGCCT GACTGTGAAG 
GGCCCCAGAC ATAAGTCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGACGTT 
CACAGTGGAA TGGCCTTACC AGCGAGCAAG AGCACCGCCA GGCTGGACAG CGACAGGACG 
CCGTCCACCT CCAGCACCGC CGAGCGGGGG ATGGTGAAGC CGATGGTCCG CGTGGAGCAG 
CAGCTGGACT ACCGCCGGCA GGACAGCAGA ACTCAGGATG CTCCCGGGCC TGACCTGATT 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CCTTTTTATC TGCCATTATA 
AATTACACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCGC CCACTTACGT GTTATACATG 
GCCTGTCGGT ATGTATTGTC CAGCCAGTAC AGACCCGACG TCAGCCCCGC GGAACGCACG 
CACAAAGTCA TGGCCATCGT CAACAAGATG GTGAGCATGA TGGAAGGGGT GATCCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGGT CCTGGTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320395.1
CDS	66..4928
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKVLVL

Target ORF information:

RefSeq Version	XM_015464909.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464909.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGGGA GAAGGAACCA CTCGGCCTGC 
TACAGCTCTC ACGCTTCCGA AGACGGTTCT GACTCCAGAG ACAAGCCAAA GCTCTACCGC 
CTTCAGCTCA GCGTCACGGA GGTGGGGACC GAGAAGTTCG ACGACAGCTC CATCCAGTTG 
TTTGGTTCAG GCATTCAGCC CCATCACTGT GACCTCACGA ATATGGACGG GGTGGTCACG 
GTCACGCCGA GAAGCATGGA CGCTGAGACC TACGTGGATG GGCAGCGCAT CTCGGAGACC 
ACGATGCTGC AGAGCGGGAT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTCGTG 
GACCCCAGCC AGGACCACAC GCTCCCCAAG AGATCCGTGG ACGGGGGCCT GACTGTGAAG 
GGCCCCAGAC ATAAGTCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGACGTT 
CACAGTGGAA TGGCCTTACC AGCGAGCAAG AGCACCGCCA GGCTGGACAG CGACAGGACG 
CCGTCCACCT CCAGCACCGC CGAGCGGGGG ATGGTGAAGC CGATGGTCCG CGTGGAGCAG 
CAGCTGGACT ACCGCCGGCA GGACAGCAGA ACTCAGGATG CTCCCGGGCC TGACCTGATT 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CCTTTTTATC TGCCATTATA 
AATTACACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCGC CCACTTACGT GTTATACATG 
GCCTGTCGGT ATGTATTGTC CAGCCAGTAC AGACCCGACG TCAGCCCCGC GGAACGCACG 
CACAAAGTCA TGGCCATCGT CAACAAGATG GTGAGCATGA TGGAAGGGGT GATCCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGGT CCTGGTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024997154.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852922.1 Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X19, mRNA.

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CloneID	OBa94648
Clone ID Related Accession (Same CDS sequence)	XM_015464909.1 , XM_024997154.1
Accession Version	XM_024997154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	afadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGGGA GAAGGAACCA CTCGGCCTGC 
TACAGCTCTC ACGCTTCCGA AGACGGTTCT GACTCCAGAG ACAAGCCAAA GCTCTACCGC 
CTTCAGCTCA GCGTCACGGA GGTGGGGACC GAGAAGTTCG ACGACAGCTC CATCCAGTTG 
TTTGGTTCAG GCATTCAGCC CCATCACTGT GACCTCACGA ATATGGACGG GGTGGTCACG 
GTCACGCCGA GAAGCATGGA CGCTGAGACC TACGTGGATG GGCAGCGCAT CTCGGAGACC 
ACGATGCTGC AGAGCGGGAT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTCGTG 
GACCCCAGCC AGGACCACAC GCTCCCCAAG AGATCCGTGG ACGGGGGCCT GACTGTGAAG 
GGCCCCAGAC ATAAGTCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGACGTT 
CACAGTGGAA TGGCCTTACC AGCGAGCAAG AGCACCGCCA GGCTGGACAG CGACAGGACG 
CCGTCCACCT CCAGCACCGC CGAGCGGGGG ATGGTGAAGC CGATGGTCCG CGTGGAGCAG 
CAGCTGGACT ACCGCCGGCA GGACAGCAGA ACTCAGGATG CTCCCGGGCC TGACCTGATT 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CCTTTTTATC TGCCATTATA 
AATTACACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCGC CCACTTACGT GTTATACATG 
GCCTGTCGGT ATGTATTGTC CAGCCAGTAC AGACCCGACG TCAGCCCCGC GGAACGCACG 
CACAAAGTCA TGGCCATCGT CAACAAGATG GTGAGCATGA TGGAAGGGGT GATCCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGGT CCTGGTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852922.1
CDS	72..4934
Translation	MSAGGRDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVLETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAVPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFSGDDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPRDYCIARVMLPPGAQHSDEKGAKEVILDDDECPLQIFREWPSDRGILVFQLKRRPPDHIPKKSKKHTDGKPLQGKERADGSAYGSALPPEKLPYLVELSPGRRNHSACYSSHASEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHTLPKRSVDGGLTVKGPRHKSGIVQETTFDLGGDVHSGMALPASKSTARLDSDRTPSTSSTAERGMVKPMVRVEQQLDYRRQDSRTQDAPGPDLILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPAERTHKVMAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEEDSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAILRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPADLIANVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNVPSGLQEFLDPLCQRGFCRLIPHPRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISERRGSGKPRPKSEGFELYNNSAQNGSPESPQTPWAEYSEPKKLPGDDRLTKHRADHRSSPNVANQPPSPGGKSAYASGTAAKITSVSTGNLCAEEQTPPPRPEAYPIPTQTSTREYFTFPASKSQDRVAPPQSQWLGFEEKPHVLADGNHSSAAVQRVTRSQEELREDAYHPERQRPEAAADRRSDGSDAWPTQGSSLGSGASSQEHLGPPSKAGTPASTLTKSGPGRWKTPAASPPMRTDPALPPPPPPGSYAGEADADADSPPPPAATAAGQAQAAAERKKREEQQRWYEKEKARLDEERERRRREQERRLGQLRAPAPPPAPARAPDTVIRELQPQQQPRTIERRDLQYITVSREELASGDSLSPDPWKRDAREKLEKQQQLHIVDLLSREIQELQGKAERSAEESDRLRKLLLEWQFQKRLQESKQKDEDDEEEEDDDVDTVLLMQRLEAERRARDEERRRQQQLEEMRTREAEERARQEEQRRRQEDERVRREAEDKVLVL

Target ORF information:

RefSeq Version	XM_024997154.1
Organism	Bos taurus(cattle)
Definition	Bos taurus afadin, adherens junction formation factor (AFDN), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997154.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GACTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTCCAC 
GGAGTGATGA GGTTTTATTT TCAAGATAAA GCCGCCGGGA ACTTCGCCAC GAAATGCATC 
CGGGTGTCCA GCACCGCCAC CACCCAGGAC GTGCTGGAGA CGCTGGCCGA GAAGTTCCGC 
CCCGACATGC GCATGCTGTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCC CTGGTGGTGC AGCTGAACTG GAATAAAGAT 
GACCGAGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGATG CTGTTCCTGC AAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTCA TCCAGAACTT TAAGAGAACT 
CTCTCAAAGA AGGAGAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCAGT GAGACAGGCG 
TCTGAGAAAG ATGACAGACC TTTCTCTGGG GATGACACTG AGAATTCTCG CCTGGCCGCT 
GAGGTCTACA AAGACATGCC TGAGACCAGC TTTACGCGAA CCATCTCCAA CCCTGAGGTG 
GTCATGAAGC GGCGGCGGCA GCAGAAGCTG GAGAGGAGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CAGACTCGGG TGGAACATTG AGAATTTACG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACGATCCT GCTGTCCACT ACAGACCCTG CGGACTTTGC TGTGGCCGAA 
GCCTTGGAGA AGTACGGCCT GGAGAAGGAG AACCCCAGGG ACTACTGCAT CGCCCGTGTC 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAAAAAGGTG CTAAAGAAGT TATTCTTGAT 
GATGACGAAT GTCCTTTACA GATCTTCAGG GAATGGCCGA GTGACAGAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAAGTAAGAA GCACACGGAC 
GGGAAGCCCC TGCAGGGGAA GGAGAGAGCC GATGGGTCGG CCTACGGCTC CGCCCTGCCC 
CCCGAGAAGC TGCCCTACCT CGTGGAGCTG AGCCCGGGGA GAAGGAACCA CTCGGCCTGC 
TACAGCTCTC ACGCTTCCGA AGACGGTTCT GACTCCAGAG ACAAGCCAAA GCTCTACCGC 
CTTCAGCTCA GCGTCACGGA GGTGGGGACC GAGAAGTTCG ACGACAGCTC CATCCAGTTG 
TTTGGTTCAG GCATTCAGCC CCATCACTGT GACCTCACGA ATATGGACGG GGTGGTCACG 
GTCACGCCGA GAAGCATGGA CGCTGAGACC TACGTGGATG GGCAGCGCAT CTCGGAGACC 
ACGATGCTGC AGAGCGGGAT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTCGTG 
GACCCCAGCC AGGACCACAC GCTCCCCAAG AGATCCGTGG ACGGGGGCCT GACTGTGAAG 
GGCCCCAGAC ATAAGTCTGG AATTGTTCAG GAGACAACTT TTGATTTAGG AGGAGACGTT 
CACAGTGGAA TGGCCTTACC AGCGAGCAAG AGCACCGCCA GGCTGGACAG CGACAGGACG 
CCGTCCACCT CCAGCACCGC CGAGCGGGGG ATGGTGAAGC CGATGGTCCG CGTGGAGCAG 
CAGCTGGACT ACCGCCGGCA GGACAGCAGA ACTCAGGATG CTCCCGGGCC TGACCTGATT 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CCTTTTTATC TGCCATTATA 
AATTACACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCGC CCACTTACGT GTTATACATG 
GCCTGTCGGT ATGTATTGTC CAGCCAGTAC AGACCCGACG TCAGCCCCGC GGAACGCACG 
CACAAAGTCA TGGCCATCGT CAACAAGATG GTGAGCATGA TGGAAGGGGT GATCCAGAAA 
CAGAAGAACA TTGCAGGGGC CCTTGCCTTC TGGATGGCCA ACGCGTCCGA GCTGCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGG ATCACACTGG ATGCACAGGA CGTCCTGGCA 
CACTTGGTGC AGATGGCCTT TAAGTACCTG GTTCACTGTC TTCAGTCAGA GCTGAATAAT 
TACATGCCCG CTTTTCTGGA CGACCCCGAA GAGGACAGTC TGCAGAGACC AAAAATAGAC 
GACGTGCTGC ACACGCTCAC GGGCGCCATG TCCCTGCTGC GTCGCTGCCG GGTCAATGCC 
GCGCTGACCA TCCAGCTCTT CTCCCAACTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
CGGCTGGTGA CCGACCCCGA GTCGGGGCTC TGCTCCCACT ACTGGGGTGC CATCCTCCGC 
CAGCAGCTCG GGCACGTGGA GGCCTGGGCC GAGAAGCAGG GCCTGGAGCT GGCGGCTGAC 
TGCCACCTGA GCCGGATAGT GCAGGCAACC ACTTTGCTTA CGATGGATAA GTACGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTCAAGTTAA ATTCACTGCA GCTCCAAGCC 
TTACTGCAGA ATTACCACTG TGCACCCGAT GAGCCTTTTG TCCCTGCGGA CCTGATCGCG 
AACGTGGTGG CCGTGGCCGA GAACACAGCA GACGAGCTGG CACGCAGCGA CGGGCGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCTTTCC TCCTGCCTGA GGACGGCTAC 
TCCTGTGACG TCGTCAGGAA TGTCCCCAGC GGCCTGCAGG AGTTCCTGGA CCCTCTCTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAC CCACGCTCTC CAGGCACCTG GACGATATAT 
TTTGAAGGTG CAGATTACGA GAGTCACCTT TTGCGGGAGA ACACAGAACT GGCACAGCCT 
CTGCGTAAAG AACCTGAAAT CATCACTGTG ACCCTCAAAA AGCAGAACGG AATGGGCCTT 
AGCATTGTTG CTGCAAAGGG CGCTGGTCAA GACAAACTTG GGATTTACGT CAAGTCCGTT 
GTGAAAGGAG GCGCTGCGGA CGTGGACGGG CGGCTCGCCG CGGGTGACCA GCTGCTCAGC 
GTGGACGGAC GGAGTCTGGT GGGACTCTCT CAAGAAAGGG CAGCAGAACT GATGACAAGG 
ACAAGCTCCG TCGTGACGCT TGAAGTAGCA AAGCAAGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCTTC CCCTATGATG CAGAGAATTT CAGAGCGCCG TGGGTCAGGT 
AAACCCCGAC CAAAAAGCGA AGGCTTTGAG CTGTACAACA ACTCGGCTCA GAACGGGTCC 
CCGGAGAGCC CGCAGACGCC CTGGGCCGAG TACAGCGAGC CCAAGAAGCT GCCGGGCGAT 
GACCGGCTGA CGAAGCACAG GGCAGACCAC CGCTCCAGCC CCAACGTCGC AAATCAGCCT 
CCCAGTCCTG GCGGGAAGAG TGCGTATGCC TCCGGAACAG CAGCCAAGAT CACATCTGTC 
TCCACGGGGA ACCTCTGCGC TGAGGAGCAG ACGCCTCCGC CCCGCCCCGA AGCCTACCCC 
ATCCCCACGC AGACGTCCAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAA GTCCCAGGAC 
CGCGTGGCGC CCCCGCAGAG CCAGTGGCTG GGTTTCGAGG AGAAGCCCCA CGTGCTGGCA 
GACGGAAACC ACTCCAGCGC TGCCGTCCAG CGTGTGACCC GCTCCCAGGA GGAGCTCCGC 
GAGGACGCCT ACCACCCCGA GCGGCAGCGG CCGGAGGCAG CCGCGGATCG CAGGTCGGAT 
GGCAGCGACG CCTGGCCCAC CCAGGGCTCA TCACTGGGTT CCGGCGCGTC CAGCCAGGAG 
CACCTGGGGC CCCCCTCCAA GGCGGGCACC CCCGCCTCCA CGCTCACCAA GAGCGGCCCC 
GGGCGCTGGA AGACCCCGGC CGCCTCCCCG CCCATGCGGA CCGACCCGGC CCTGCCGCCA 
CCGCCGCCCC CCGGGTCCTA CGCCGGGGAG GCTGACGCGG ACGCGGACTC GCCCCCGCCT 
CCCGCCGCCA CCGCCGCCGG CCAGGCGCAG GCGGCCGCCG AGCGCAAGAA ACGAGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAAAAAGCG CGCCTGGACG AGGAGCGCGA GCGCCGGCGC 
CGGGAGCAGG AGCGCCGGCT GGGCCAGCTG CGCGCGCCCG CGCCGCCCCC CGCGCCCGCC 
CGCGCGCCCG ACACGGTGAT CCGCGAGCTG CAGCCGCAGC AGCAGCCGCG TACCATCGAG 
CGCAGGGACC TGCAGTACAT CACGGTCAGC AGGGAGGAGC TGGCCTCCGG AGACAGCCTG 
TCCCCCGACC CCTGGAAGCG CGATGCGCGC GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTGGACC TGCTGAGCCG CGAGATCCAG GAGCTGCAGG GCAAGGCCGA GCGCTCGGCC 
GAGGAGAGCG ACCGGCTGCG CAAGCTGCTG CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGATGACGT GGACACCGTG 
CTGCTCATGC AGCGCCTGGA GGCCGAGCGC AGAGCGCGGG ATGAGGAGCG GCGGCGGCAG 
CAGCAGCTGG AGGAGATGCG GACACGGGAG GCGGAGGAGC GCGCGCGGCA GGAGGAGCAG 
CGGCGCCGGC AGGAGGACGA GCGGGTCAGG CGCGAGGCCG AAGACAAGGT CCTGGTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

AFDN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: