PPEF1 cDNA ORF clone, Bos taurus(cattle)

The following PPEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa13101 XM_015461779.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015461778.2
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015461777.2
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015470577.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015470576.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_010822171.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_024988540.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_010800590.2
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_010800591.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_024988541.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_010822170.2
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015470575.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_024988539.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_024988545.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_015470581.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_024988546.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_015461782.2
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_015470580.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_015461783.2
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa46018 XM_015461781.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa46018 XM_015470579.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa46018 XM_024988544.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa238662 XM_024988542.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa89438 XM_024988543.1
Latest version!
Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBa89438 XM_015461780.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBa89438 XM_015470578.1
Latest version!
Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OBa13102 XM_015461782.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015461777.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13101 XM_015461778.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa13102 XM_015461783.1 Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_010822170.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820472.1
    CDS506..2482
    Translation

    Target ORF information:

    RefSeq Version XM_010822170.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822170.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_010800590.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010798892.1
    CDS506..2482
    Translation

    Target ORF information:

    RefSeq Version XM_010800590.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800590.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_010800591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010798893.1
    CDS238..2214
    Translation

    Target ORF information:

    RefSeq Version XM_010800591.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_024988541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844309.1
    CDS372..2348
    Translation

    Target ORF information:

    RefSeq Version XM_024988541.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_010822171.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820473.1
    CDS238..2214
    Translation

    Target ORF information:

    RefSeq Version XM_010822171.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015461778.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317264.1
    CDS888..2864
    Translation

    Target ORF information:

    RefSeq Version XM_015461778.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461778.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015461777.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317263.1
    CDS814..2790
    Translation

    Target ORF information:

    RefSeq Version XM_015461777.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461777.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015461777.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317263.1
    CDS572..2548
    Translation

    Target ORF information:

    RefSeq Version XM_015461777.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461777.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015461778.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317264.1
    CDS646..2622
    Translation

    Target ORF information:

    RefSeq Version XM_015461778.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015470575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326061.1
    CDS572..2548
    Translation

    Target ORF information:

    RefSeq Version XM_015470575.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015470576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326062.1
    CDS646..2622
    Translation

    Target ORF information:

    RefSeq Version XM_015470576.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015470577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326063.1
    CDS420..2396
    Translation

    Target ORF information:

    RefSeq Version XM_015470577.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_015461779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317265.1
    CDS420..2396
    Translation

    Target ORF information:

    RefSeq Version XM_015461779.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_024988539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844307.1
    CDS891..2867
    Translation

    Target ORF information:

    RefSeq Version XM_024988539.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13101
    Clone ID Related Accession (Same CDS sequence) XM_010822170.2 , XM_010800590.2 , XM_010800591.1 , XM_024988541.1 , XM_010822171.1 , XM_015461778.2 , XM_015461777.2 , XM_015461777.1 , XM_015461778.1 , XM_015470575.1 , XM_015470576.1 , XM_015470577.1 , XM_015461779.1 , XM_024988539.1 , XM_024988540.1
    Accession Version XM_024988540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844308.1
    CDS817..2793
    Translation

    Target ORF information:

    RefSeq Version XM_024988540.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGGTTCAGT CTTCTCTAAT TGAAACAGTG TACAGATACC GATCTGACCT GCAAATCATC
    TTTAATATCA TTGACTCTGA TCACTCAGGC CTGATCTCCA TGGAAGAATT TCGTAGCATG
    TGGAGACTTT TCAAAAGTCA CTACAGTGTC CATATTGATG ATTCCCAGTT TGATGAGCTC
    GCCGAAAGGA TGGACTTAAA CAAAGATGGA AGCATTGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TGCATAAATT TGACCAGTTG AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_015470581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326067.1
    CDS791..2461
    Translation

    Target ORF information:

    RefSeq Version XM_015470581.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_015461782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317268.1
    CDS959..2629
    Translation

    Target ORF information:

    RefSeq Version XM_015461782.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_015461783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317269.1
    CDS1033..2703
    Translation

    Target ORF information:

    RefSeq Version XM_015461783.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_024988546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844314.1
    CDS340..2010
    Translation

    Target ORF information:

    RefSeq Version XM_024988546.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_015461782.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317268.1
    CDS717..2387
    Translation

    Target ORF information:

    RefSeq Version XM_015461782.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_024988545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844313.1
    CDS1036..2706
    Translation

    Target ORF information:

    RefSeq Version XM_024988545.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_015470580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326066.1
    CDS717..2387
    Translation

    Target ORF information:

    RefSeq Version XM_015470580.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa13102
    Clone ID Related Accession (Same CDS sequence) XM_015470581.1 , XM_015461782.2 , XM_015461783.2 , XM_024988546.1 , XM_015461782.1 , XM_024988545.1 , XM_015470580.1 , XM_015461783.1
    Accession Version XM_015461783.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317269.1
    CDS791..2461
    Translation

    Target ORF information:

    RefSeq Version XM_015461783.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCCCTCAC TATCTTCCAG TCCATCGAAT ACGCTGATGA GCAAGGCCAG TTGCAGCTCT 
    CAAACTTCTT CTCCTTCATG TTGGAAAACT ATACAAATGT GCCGGAGAAA GAGCCAGATA
    CTTCATGCTT ATTATGTCTT AGAGGTGCTG TTTGAAACCA AGAAAGTCCT GCAGCAAATG
    CCGAATTTCA CTCATACAAA CACATCTCCA TCCAAAGAGA TAACAATCTG TGGTGATTTG
    CATGGGAAGC TAGATGACCT TTTTCTGATC TTCTATAAGA ATGGTCTCCC CTCAGTGACC
    AACCCATATG TTTTTAATGG TGATTTTGTA GATCGAGGAA AAAATTCCAT GGAGATCCTA
    ATGATCCTGT TTGTGAGTTT TCTTGTGTAC CCAAATGACC TGCACTTGAA CAGAGGCAAC
    CATGAGGATT TTATGATGAA TATGAGGTAT GGCTTCACGA AAGAAATTTT ACATAAATAT
    AAGCTGCATG GAAAAAAAAT CTTACAAATC TTGGAAGAGG TCTATACCTG GCTCCCAATT
    GGTACAATCA TTGACAATGA AGTCCTGGTC ATACATGGAG GGATATCAGA GTCCACAGAC
    TTGAATTTAC TCCACCGTAT AGAAAGAAAC AAAATGAGAT CTGTGCTGAT GCCGCCAATT
    CCAATGGATG GAGACCGTGC TGAGAAGAAA AATAAACTGG GTACAGCCAC TGTGGCTCTG
    GGAATCAGAA CAAGTGGATC CTTTTCTGAA CAATTAACAA AGCAGGAATG GGAGCAGGTT
    ATCGATATCC TTTGGAGTGA TCCCAGAGGC AAAAGAGGCT GCTACCCAAA CACGAACCGA
    GGAGGGGGCT GCTACTTTGG ACCAGACATT ACCTCCAAGA TTCTTAATAA ATACCAGTTG
    AAGATGCTTG TTAGATCTCA TGAGTGTAAG CCTGAAGGGT ATGAAATCTG CCACGATGGG
    AAGGTTATTA CTGTATTTTC TGCGTCTAAT TATTATGAAG AAGGTAGCAA TCGAGGAGCT
    TACATCAGAA TGAGTTCTGG TACGACCCCT CGATTTTTCC AGTACCAAAT AACTAAGGCA
    ACATGCTTCA GGCCTCTTTA CCAAAGGGTG AATGCTATTG AAAGTAGTGC CATCAGGATG
    TTAAAAGAGA GAATGATTTC ACGAAAAACT GACCTCATTC GAGCTTTCCA ACTTCAAGAC
    CGCAATAAAT CAGGAAAACT TTCAATGGGC CAGTGGGCTT TTTCAATGGA GAATGTTTTG
    GGGCTGAACT TACCATGGAG ATCACTGAGT TCTCATCTGG TAACCACAGA CAAAGATGGA
    AATATTGACT ACATGTCTGG CTTCCAGGAT GTCCACATTC AGAAACCTGT GAAAGAGGTT
    CAGTCTTCTC TAATTGAAAC AGTGTACAGA TACCGATCTG ACCTGCAAAT CATCTTTAAT
    ATCATTGACT CTGATCACTC AGGCCTGATC TCCATGGAAG AATTTCGTAG CATGTGGAGA
    CTTTTCAAAA GTCACTACAG TGTCCATATT GATGATTCCC AGTTTGATGA GCTCGCCGAA
    AGGATGGACT TAAACAAAGA TGGAAGCATT GACTTTAATG AGTTTTTAAA GGCTTTCTAT
    GTAGTGCATA AATTTGACCA GTTGAACAAA TCAGACACCA AGCTTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46018
    Clone ID Related Accession (Same CDS sequence) XM_015461781.1 , XM_015470579.1 , XM_024988544.1
    Accession Version XM_015461781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGATTCACT TTGGACCATC CTCTGAGGAA CATAGGCTGA AGACACTACA GAACACAAGA
    GCACGGTCTT TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317267.1
    CDS572..2329
    Translation

    Target ORF information:

    RefSeq Version XM_015461781.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGATTCACT TTGGACCATC CTCTGAGGAA CATAGGCTGA AGACACTACA GAACACAAGA
    GCACGGTCTT TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46018
    Clone ID Related Accession (Same CDS sequence) XM_015461781.1 , XM_015470579.1 , XM_024988544.1
    Accession Version XM_015470579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGATTCACT TTGGACCATC CTCTGAGGAA CATAGGCTGA AGACACTACA GAACACAAGA
    GCACGGTCTT TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326065.1
    CDS572..2329
    Translation

    Target ORF information:

    RefSeq Version XM_015470579.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGATTCACT TTGGACCATC CTCTGAGGAA CATAGGCTGA AGACACTACA GAACACAAGA
    GCACGGTCTT TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46018
    Clone ID Related Accession (Same CDS sequence) XM_015461781.1 , XM_015470579.1 , XM_024988544.1
    Accession Version XM_024988544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGATTCACT TTGGACCATC CTCTGAGGAA CATAGGCTGA AGACACTACA GAACACAAGA
    GCACGGTCTT TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844312.1
    CDS891..2648
    Translation

    Target ORF information:

    RefSeq Version XM_024988544.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGC TGCATGGAAA AAAAATCTTA CAAATCTTGG AAGAGGTCTA TACCTGGCTC
    CCAATTGGTA CAATCATTGA CAATGAAGTC CTGGTCATAC ATGGAGGGAT ATCAGAGTCC
    ACAGACTTGA ATTTACTCCA CCGTATAGAA AGAAACAAAA TGAGATCTGT GCTGATGCCG
    CCAATTCCAA TGGATGGAGA CCGTGCTGAG AAGAAAAATA AACTGGGTAC AGCCACTGTG
    GCTCTGGGAA TCAGAACAAG TGGATCCTTT TCTGAACAAT TAACAAAGCA GGAATGGGAG
    CAGGTTATCG ATATCCTTTG GAGTGATCCC AGAGGCAAAA GAGGCTGCTA CCCAAACACG
    AACCGAGGAG GGGGCTGCTA CTTTGGACCA GACATTACCT CCAAGATTCT TAATAAATAC
    CAGTTGAAGA TGCTTGTTAG ATCTCATGAG TGTAAGCCTG AAGGGTATGA AATCTGCCAC
    GATGGGAAGG TTATTACTGT ATTTTCTGCG TCTAATTATT ATGAAGAAGG TAGCAATCGA
    GGAGCTTACA TCAGAATGAG TTCTGGTACG ACCCCTCGAT TTTTCCAGTA CCAAATAACT
    AAGGCAACAT GCTTCAGGCC TCTTTACCAA AGGGTGAATG CTATTGAAAG TAGTGCCATC
    AGGATGTTAA AAGAGAGAAT GATTTCACGA AAAACTGACC TCATTCGAGC TTTCCAACTT
    CAAGACCGCA ATAAATCAGG AAAACTTTCA ATGGGCCAGT GGGCTTTTTC AATGGAGAAT
    GTTTTGGGGC TGAACTTACC ATGGAGATCA CTGAGTTCTC ATCTGGTAAC CACAGACAAA
    GATGGAAATA TTGACTACAT GTCTGGCTTC CAGGATGTCC ACATTCAGAA ACCTGTGAAA
    GAGATTCACT TTGGACCATC CTCTGAGGAA CATAGGCTGA AGACACTACA GAACACAAGA
    GCACGGTCTT TTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238662
    Clone ID Related Accession (Same CDS sequence) XM_024988542.1
    Accession Version XM_024988542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTGGCATT TTGGTCGGAG GGACTCGGGA CCTATTGAGG TCGCACTAAA AGCCGCATTG 
    GTCATACAGA ACTGGTACCG AGGTTACAGC GCCCGGTTGA AGACCAGACA GCGCTATGCC
    CTCACTATCT TCCAGTCCAT CGAATACGCT GATGAGCAAG GCCAGTTGCA GCTCTCAAAC
    TTCTTCTCCT TCATGTTGGA AAACTATACA AATGTGCCGG AGAAAGAGCC AGGATTAGTA
    ACTCGCCTTT TTGGTAGCAG CATCCAGGAC AAGGATAGAC AAGACTATTT GGGGTTGATA
    GAAATCCCAG ACTCCTACTG TGGTCCTCGG CTGCAATTTC CTCTCACTTT TACGGACATT
    GATTTACTTA TTGAGGCCTT CAAGCAACAA CAGATACTTC ATGCTTATTA TGTCTTAGAG
    GTGCTGTTTG AAACCAAGAA AGTCCTGCAG CAAATGCCGA ATTTCACTCA TACAAACACA
    TCTCCATCCA AAGAGATAAC AATCTGTGGT GATTTGCATG GGAAGCTAGA TGACCTTTTT
    CTGATCTTCT ATAAGAATGG TCTCCCCTCA GTGACCAACC CATATGTTTT TAATGGTGAT
    TTTGTAGATC GAGGAAAAAA TTCCATGGAG ATCCTAATGA TCCTGTTTGT GAGTTTTCTT
    GTGTACCCAA ATGACCTGCA CTTGAACAGA GGCAACCATG AGGATTTTAT GATGAATATG
    AGGTATGGCT TCACGAAAGA AATTTTACAT AAATATAAGC TGCATGGAAA AAAAATCTTA
    CAAATCTTGG AAGAGGTCTA TACCTGGCTC CCAATTGGTA CAATCATTGA CAATGAAGTC
    CTGGTCATAC ATGGAGGGAT ATCAGAGTCC ACAGACTTGA ATTTACTCCA CCGTATAGAA
    AGAAACAAAA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844310.1
    CDS228..2174
    Translation

    Target ORF information:

    RefSeq Version XM_024988542.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTGGCATT TTGGTCGGAG GGACTCGGGA CCTATTGAGG TCGCACTAAA AGCCGCATTG 
    GTCATACAGA ACTGGTACCG AGGTTACAGC GCCCGGTTGA AGACCAGACA GCGCTATGCC
    CTCACTATCT TCCAGTCCAT CGAATACGCT GATGAGCAAG GCCAGTTGCA GCTCTCAAAC
    TTCTTCTCCT TCATGTTGGA AAACTATACA AATGTGCCGG AGAAAGAGCC AGGATTAGTA
    ACTCGCCTTT TTGGTAGCAG CATCCAGGAC AAGGATAGAC AAGACTATTT GGGGTTGATA
    GAAATCCCAG ACTCCTACTG TGGTCCTCGG CTGCAATTTC CTCTCACTTT TACGGACATT
    GATTTACTTA TTGAGGCCTT CAAGCAACAA CAGATACTTC ATGCTTATTA TGTCTTAGAG
    GTGCTGTTTG AAACCAAGAA AGTCCTGCAG CAAATGCCGA ATTTCACTCA TACAAACACA
    TCTCCATCCA AAGAGATAAC AATCTGTGGT GATTTGCATG GGAAGCTAGA TGACCTTTTT
    CTGATCTTCT ATAAGAATGG TCTCCCCTCA GTGACCAACC CATATGTTTT TAATGGTGAT
    TTTGTAGATC GAGGAAAAAA TTCCATGGAG ATCCTAATGA TCCTGTTTGT GAGTTTTCTT
    GTGTACCCAA ATGACCTGCA CTTGAACAGA GGCAACCATG AGGATTTTAT GATGAATATG
    AGGTATGGCT TCACGAAAGA AATTTTACAT AAATATAAGC TGCATGGAAA AAAAATCTTA
    CAAATCTTGG AAGAGGTCTA TACCTGGCTC CCAATTGGTA CAATCATTGA CAATGAAGTC
    CTGGTCATAC ATGGAGGGAT ATCAGAGTCC ACAGACTTGA ATTTACTCCA CCGTATAGAA
    AGAAACAAAA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89438
    Clone ID Related Accession (Same CDS sequence) XM_024988543.1 , XM_015470578.1 , XM_015461780.1
    Accession Version XM_024988543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844311.1
    CDS891..2717
    Translation

    Target ORF information:

    RefSeq Version XM_024988543.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89438
    Clone ID Related Accession (Same CDS sequence) XM_024988543.1 , XM_015470578.1 , XM_015461780.1
    Accession Version XM_015470578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326064.1
    CDS572..2398
    Translation

    Target ORF information:

    RefSeq Version XM_015470578.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89438
    Clone ID Related Accession (Same CDS sequence) XM_024988543.1 , XM_015470578.1 , XM_015461780.1
    Accession Version XM_015461780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317266.1
    CDS572..2398
    Translation

    Target ORF information:

    RefSeq Version XM_015461780.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACACAC CTAATTTAGA AGACATGGGC TGTGGCACTT CATCTGCAAC AAAAGACAAG 
    AAATCTGAGA AAGCACTAAA AGCCGCATTG GTCATACAGA ACTGGTACCG AGGTTACAGC
    GCCCGGTTGA AGACCAGACA GCGCTATGCC CTCACTATCT TCCAGTCCAT CGAATACGCT
    GATGAGCAAG GCCAGTTGCA GCTCTCAAAC TTCTTCTCCT TCATGTTGGA AAACTATACA
    AATGTGCCGG AGAAAGAGCC AGGATTAGTA ACTCGCCTTT TTGGTAGCAG CATCCAGGAC
    AAGGATAGAC AAGACTATTT GGGGTTGATA GAAATCCCAG ACTCCTACTG TGGTCCTCGG
    CTGCAATTTC CTCTCACTTT TACGGACATT GATTTACTTA TTGAGGCCTT CAAGCAACAA
    CAGATACTTC ATGCTTATTA TGTCTTAGAG GTGCTGTTTG AAACCAAGAA AGTCCTGCAG
    CAAATGCCGA ATTTCACTCA TACAAACACA TCTCCATCCA AAGAGATAAC AATCTGTGGT
    GATTTGCATG GGAAGCTAGA TGACCTTTTT CTGATCTTCT ATAAGAATGG TCTCCCCTCA
    GTGACCAACC CATATGTTTT TAATGGTGAT TTTGTAGATC GAGGAAAAAA TTCCATGGAG
    ATCCTAATGA TCCTGTTTGT GAGTTTTCTT GTGTACCCAA ATGACCTGCA CTTGAACAGA
    GGCAACCATG AGGATTTTAT GATGAATATG AGGTATGGCT TCACGAAAGA AATTTTACAT
    AAATATAAGA TGAGATCTGT GCTGATGCCG CCAATTCCAA TGGATGGAGA CCGTGCTGAG
    AAGAAAAATA AACTGGGTAC AGCCACTGTG GCTCTGGGAA TCAGAACAAG TGGATCCTTT
    TCTGAACAAT TAACAAAGCA GGAATGGGAG CAGGTTATCG ATATCCTTTG GAGTGATCCC
    AGAGGCAAAA GAGGCTGCTA CCCAAACACG AACCGAGGAG GGGGCTGCTA CTTTGGACCA
    GACATTACCT CCAAGATTCT TAATAAATAC CAGTTGAAGA TGCTTGTTAG ATCTCATGAG
    TGTAAGCCTG AAGGGTATGA AATCTGCCAC GATGGGAAGG TTATTACTGT ATTTTCTGCG
    TCTAATTATT ATGAAGAAGG TAGCAATCGA GGAGCTTACA TCAGAATGAG TTCTGGTACG
    ACCCCTCGAT TTTTCCAGTA CCAAATAACT AAGGCAACAT GCTTCAGGCC TCTTTACCAA
    AGGGTGAATG CTATTGAAAG TAGTGCCATC AGGATGTTAA AAGAGAGAAT GATTTCACGA
    AAAACTGACC TCATTCGAGC TTTCCAACTT CAAGACCGCA ATAAATCAGG AAAACTTTCA
    ATGGGCCAGT GGGCTTTTTC AATGGAGAAT GTTTTGGGGC TGAACTTACC ATGGAGATCA
    CTGAGTTCTC ATCTGGTAAC CACAGACAAA GATGGAAATA TTGACTACAT GTCTGGCTTC
    CAGGATGTCC ACATTCAGAA ACCTGTGAAA GAGGTTCAGT CTTCTCTAAT TGAAACAGTG
    TACAGATACC GATCTGACCT GCAAATCATC TTTAATATCA TTGACTCTGA TCACTCAGGC
    CTGATCTCCA TGGAAGAATT TCGTAGCATG TGGAGACTTT TCAAAAGTCA CTACAGTGTC
    CATATTGATG ATTCCCAGTT TGATGAGCTC GCCGAAAGGA TGGACTTAAA CAAAGATGGA
    AGCATTGACT TTAATGAGTT TTTAAAGGCT TTCTATGTAG TGCATAAATT TGACCAGTTG
    AACAAATCAG ACACCAAGCT TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.