SI cDNA ORF clone, Bos taurus(cattle)

The following SI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218629 NM_001114189.2
Latest version!
Bos taurus sucrase-isomaltase (SI), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218629 XM_005201715.4
Latest version!
Bos taurus sucrase-isomaltase (SI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218630 XM_015472666.2
Latest version!
Bos taurus sucrase-isomaltase (SI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218630 XM_015472667.2
Latest version!
Bos taurus sucrase-isomaltase (SI), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa87784 XM_015472667.1
Latest version!
Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa87784 XM_015472666.1
Latest version!
Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa87785 XM_015472670.1
Latest version!
Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa15892 NM_001114189.1
Latest version!
Bos taurus sucrase-isomaltase (SI), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa15892 XM_005201715.3
Latest version!
Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218629
    Clone ID Related Accession (Same CDS sequence) NM_001114189.2 , XM_005201715.4
    Accession Version NM_001114189.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000001.1. On May 29, 2018 this sequence version replaced NM_001114189.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145482, SAMN04019640 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAATCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107661.2
    CDS1..5439
    Translation

    Target ORF information:

    RefSeq Version NM_001114189.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (SI), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114189.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAATCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218629
    Clone ID Related Accession (Same CDS sequence) NM_001114189.2 , XM_005201715.4
    Accession Version XM_005201715.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201715.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAATCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201772.2
    CDS87..5525
    Translation

    Target ORF information:

    RefSeq Version XM_005201715.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (SI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201715.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAATCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218630
    Clone ID Related Accession (Same CDS sequence) XM_015472667.2 , XM_015472666.2
    Accession Version XM_015472667.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAATCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328153.2
    CDS112..5289
    Translation

    Target ORF information:

    RefSeq Version XM_015472667.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (SI), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472667.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAATCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218630
    Clone ID Related Accession (Same CDS sequence) XM_015472667.2 , XM_015472666.2
    Accession Version XM_015472666.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAATCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328152.2
    CDS214..5391
    Translation

    Target ORF information:

    RefSeq Version XM_015472666.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (SI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472666.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAATCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87784
    Clone ID Related Accession (Same CDS sequence) XM_015472666.1 , XM_015472667.1
    Accession Version XM_015472666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAGTCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328152.1
    CDS242..5419
    Translation

    Target ORF information:

    RefSeq Version XM_015472666.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAGTCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87784
    Clone ID Related Accession (Same CDS sequence) XM_015472666.1 , XM_015472667.1
    Accession Version XM_015472667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAGTCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328153.1
    CDS125..5302
    Translation

    Target ORF information:

    RefSeq Version XM_015472667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCCAAGT GTACTGAACG ATCCTATCAA CCACAGAATA AACTGCATTC CAGAACGATC 
    TCCCACACAG GAGTTGAAGC CAAATTAAAA AGGAAAGCTT CACCTACACT CTTTGGAAAT
    GACATTAACA GCGTTCTCCT CACAACTCAA AGTCAGACAC GCAATCGTGT GCGGTTCAAG
    ATCACTGATC CAAATAATAG AAGATATGAA GTTCCTCATC AGTTTGTAAA AGAATTTAGT
    GGAACTGCAG CTTCTGATCC ACTGTATAAT GTGGAGGTTA TACATGATCC ATTTAGCATT
    AAAATTTCTA GAAGAAGCAA TAGTAAAATT TTGTTTGACA CCAGCATTGG TCCCCTGGTA
    TACTCTGACC AGTATTTACA GATCTCAGCC AGACTTCCCA ATGAATACAT TTATGGTCTC
    GGGGAACATA TTCATAAGAG ATTTCGCCAT GATTTATATT GGAAAACATG GCCAATTTTT
    ACTCGGGATC AACTTCCTGG TGATAATAAT AATAATTTAT ATGGCCATCA AACATTCTTC
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAGTCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87785
    Clone ID Related Accession (Same CDS sequence) XM_015472670.1
    Accession Version XM_015472670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA 
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAGTCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328156.1
    CDS140..4777
    Translation

    Target ORF information:

    RefSeq Version XM_015472670.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGCATTG AGGATACTTC TGGAAAGTCA TTTGGTGTTT TCTTAATGAA CAGCAATGCA 
    ATGGAGATTT TCATCCAACC TACTCCAATA GTAACTTACC GAGTTATAGG TGGAATTCTG
    GACTTTTATA TCTTTCTGGG AGACACACCA GAACAAGTAG TTCAACAGTA TCAAGAGCTC
    ATTGGACGAC CAGCAATGCC AGCTTATTGG AATCTTGGAT TTCAGCTGAG TCGCTGGAAT
    TACAAGACAC TTGATGTAGT GAAAGCAGTG GTACAAAGAA ATCGAGATGC TGGCATACCA
    TTTGATACAC AAATCACTGA TATTGACTAT ATGGAAGACA AGAAAATCTT TACTTATGAT
    CAAGTTACCT TTAACGGCCT CCCAGAATTT GTTCAAGATT TGCATGCTCA TGGGCAGAAA
    TACGTCATCA TCTTGGATCC TGCAGTTTCC ATAGAGAAAC GTGCGAATGG GGCAGCATAT
    GAATCCTATG ATAGGGGAAC TGCCCAAAAG GTGTGGGTAA ATGAGTCAGA TGGGACTACA
    GCAATTATTG GAGAGGTATG GCCAGGATTA ACAGTATACC CTGATTTCAC AAATCCAAGC
    TGCATAGAAT GGTGGGCAAA TGAATGCTAC CTTTTTCATC AACAAGTAAA TTATGATGGA
    CTTTGGATTG ACATGAATGA AGTTTCCAGC TTTGTTCAAG GTTCAAAGAA AGGATGCAGT
    GCAAACAACC TGAATTACCC ACCTTTTACT CCCGATATTC TTGACAAACT CATGTATTCC
    AAAACAATTT GCATGGATGC TATGCAATAC TGGGGAAAAC AGTATGATGT ACACAGCCTC
    TATGGATATA GTATGTCTAT AGCTACAGAG AAAGCTATAG AAAAAGTTCT TCCTAATAAA
    AGAAGCTTTA TTCTTACCCG ATCAACTTTT GCTGGATCTG GAAGCCATTC TGCACATTGG
    TTAGGAGACA ATACTGCTTC TTGGGAACAA ATGGAATGGT CTATTACAGG AATGCTGGAG
    TTTGGTTTGT TTGGAATACC TTTGGTTGGA GCAGATATCT GTGGGTTTGT GGCTGAGACC
    ACAGAAGAGC TTTGCAGAAG GTGGATGCAA CTTGGAGCTT TTTATCCGTA TGCCAGAAAC
    CATAACGCTG ATGGATATGA GCATCAGGAT CCAGCATTTT TTGGAAAGGA TTCTCTTTTG
    GTTAAATCAT CAAAACACTA TTTGAATATT CGCTATGCCT TGTTACCTTT CCTTTATACT
    CTATTTTATA AAGCCCATAT GTTTGGAGAA ACAGTAGCAA GGCCTTTTCT TCATGAGTTT
    TATGAGGATA CTAATAGCTG GATTGAAGAT ACTCAGTTCT TATGGGGCCC CTCATTACTT
    ATAACCCCTG TATTGAGACA GGGTGCAGAA ACAGTGAGTG CATACATCCC TGATGCCACT
    TGGTATGACT TTGAAACTGG TGTAAAAAAG CCATGGAGAA AACAACGTGT TGATATGCAG
    CTTCCAGCAG ACAAAATAGG ATTACATCTT AGAGGCGGTT ACATTATCCC CTTTCAACAA
    CCTGCTGTAA CTACAACAGC AAGCCGAAAG AACCCTCTAG GACTTATAAT TGCATTAAAT
    GATAATAACA CAGCAAAAGG AGACTTTTTC TGGGATGATG GAGAAACTAA AGATACCATC
    CAAAAGGGCA ACTACATTTT ATATACATTT TCAGTTTCTA ATAACAAGCT AGATATCACA
    TGTATGCATT CATCGTACCC AGAAGGAACT GCTTTAGCAT TTGAGACTAT AAAAGTCCTT
    GGGTTGATAA ACACTGTTAC AGAAGTTTCA GTGATAGAAG ATAATCAACC ATTGAGAGCT
    CATTACAATT TCAGTTATGA TGCTTCCAAC CAGATTCTAC TAATTCACAA TCTGACATTT
    AACCTTGGAG GAAACTTTAC TGTTCAATGG GATCAAACTT TCTTGGAAGT TGAAAAGTTT
    ACTTGTTATC CAGATTCAAA CACTGCAACT AAAGAACTCT GCGAAGAACG TGGCTGCTTA
    TGGCAAACGG TTCATTTAGG ATCCAAAGCA CCTGAGTGTT ACTTTCCTAA AGAAGACAAC
    TCTTATTTAG TCCACTCAAC TCAGTACTCA TCAGTGGGTA TAACAGCTGA CCTCCAGCTG
    AATACTGCAA ATCGAATAAA GCTACCTTCT GATTCCATTT CGTCTCTTCG TGTGGAGGTG
    AAATATCACA AAGATGATAT GTTGCAGTTT AAGATTTATG ATGCCCAAAA TAAGAGATAT
    GAAGTTCCAG TACCATTGAA CATTCCAAAT ACACCAACAA GTACTTATGA AACTAGACTT
    TATGATGTTG AAATCAAGGA AAATCCTTTT GGCATTCAGA TTCGAAGAAG AAGCACAAGA
    AGAGTTATTT GGGATTCTCG CCTTCCTGGT TTTGCTTTTA ACAACCAGTT CATTCAGATA
    TCTACCCGCC TGCCTTCAGA ATATGTGTAT GGTTTTGGGG AAATGGAACA CACAACATTT
    AAGCGTGACC TGAACTGGCA TACTTGGGGA ATGTTCACAA GAGACCAACC CCCTGGTTAC
    AAACTTAATT CCTATGGATT TCATCCCTAT TACATGGCTC TGGAAGATGA GAACAATGCT
    CATGGAGTTC TCTTACTTAA CAGCAATGCA ATGGATGTTA CATTTCAGCC AATGCCTGCT
    CTGACATATC GCATGATTGG AGGAATCTTG GACTTTTATA TGTTTTTGGG CCCATCTCCA
    GAAGTCACAA CAAAGCAATA CCATGAAGTA ATCGGCCAAC CAGTCATGCC ACCTTATTGG
    GCTTTGGGAT TCCAGTTATG TCGTTATGGA TACAGAAATA CTTCACAAGT TGAAGAAGTA
    TATAATGACA TGGTGGCTGC CCAGATCCCC TATGATGTTC AGTACACAGA TATTGATTAC
    ATGGAAAGGC AACTAGACTT TACAATTGAT GATGAATTTC ATGACCTTCC TCAGTTTGTT
    GACAAAATAA GAAGTGAGGG AATGAGATAC ATTATTATCC TGGATCCAGC AATTTCAGGA
    AATGAAACGA AGCCTTACCC TGCCTTTGAA AGAGGACAGG AGAAAGATGT CTTTGTCAAA
    TGGCCTAACA CAAGTGATAT TTGTTGGGCA AAGGTTAGGC CAGATTTGCC TAATGTGACA
    ATAGATCAAA GTCTAACTGA AGATGAAGCT GCTAATGCTA CCAAAGCTCA TGCAGCTTTT
    CCAGATTTCT TCAGGAATTC CACAGCAGAG TGGTGGGCAA GGGAAATTAT AGATTTTTAT
    AATAACCAGA TGAAGTTTGA TGGATTGTGG ATTGATATGA ATGAACCATC AAGTTTTGTA
    AATGGAACAA CTACTAATCA ATGCAGAAAT GCAGAGCTGA ATTATCCACC TTATTTCCCA
    GAACTCACAA AAAGAACTGA TGGATTACAT TTCAGAACCG TGTGTATGGA AACAGAGCAG
    ATTCTTAGTG ATGGATCATC TGTTTTGCAT TATGATGTTC ACAATCTATA TGGATGGTCA
    CAGCTGAAAC CAACTTATGA TGCATTGCAA AAGGCAACTG GCAAAAGAGG AATTATTATT
    TCTCGTTCCA CATATCCCTC TGCTGGACGA TGGTCAGGAC ACTGGCTTGG AGACAACTAT
    GCAACATGGG ACAATTTGGA AAAATCTATC ATTGGTATGA TGGAATTTAG TCTCTTTGGA
    ATGTCATATA CTGGAGCAGA TATCTGTGGT TTCTTCAACA ATTCAGAATA TCAGCTTTGT
    GCCCGCTGGA TGCAACTTGG AGCATTTTAT CCATATGCAA GAAATCACAA CAGTGCATTT
    ACTAGAAGAC AAGATCCTGC TTCCTGGAAT AAAACTTTTT CTGCAATGTC AAGGGATATT
    CTAAATATTA GATACACTTT ATTGCCCTAC TTCTATACAC AGATGTATGA AATTCATGCT
    CATGGTGGCA CTGTTATCCG ACCCCTTTTG CATGAGTTTT TAAATGAAAA ACCAACCTGG
    GATATATTCA AACAGTTTCT GTGGGGTCCA GCATTTATGG TTACTCCTGT ATTGGAACCT
    TACGTTGACA CTGTAGAAGG CTATGTCCCT AATGCTCGGT GGTTTGATTA CCATACAGGC
    AAAGATATTG GTGTCAGAGA AACATTTCAT ACATTTGCAG CTCCTTTATA TGAAATAAAC
    CTACATGTCC GTGGTGGTTA CATCCTACCA TGTCAAGAGC CTGCTAACAA TACATTTTAC
    AGCCGACAGA ATTACATGAA GCTCATTGTT GCTGCAGATG ATAATCAGAT GGCACAAGGA
    TCTCTGTTTT GGGATGATGG AGAGACTATT GACACCTATG AAAGAGACCT ATATTCCTTG
    GTACAATTTA ATTTAAATAA GACCATCTTG ACGAGCACTA TACTGAAAAA TGGTTACATA
    AACAGAAATG AAATGAGGCT TGGATATATC CACATATGGG GGAAAGAAAA GATGCGTGTT
    AGTGAGGTTA CTCTCACACA CAACGGAAAT AAAGAGTCTG TTAATTTTAC TGAAGACCCA
    AACCTGGAGA TATTAGACAT TGATCTAACA GGGAATAATG TTACGCTAGA TGAACCAATA
    GAAGTCAACT GGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15892
    Clone ID Related Accession (Same CDS sequence) XM_005201715.3 , NM_001114189.1
    Accession Version NM_001114189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02002302.1, DAAA02002303.1, DAAA02002304.1 and DAAA02002305.1. On Feb 2, 2008 this sequence version replaced XM_580476.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019640, SAMN04019647 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAGTCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107661.1
    CDS1..5439
    Translation

    Target ORF information:

    RefSeq Version NM_001114189.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (SI), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAGTCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15892
    Clone ID Related Accession (Same CDS sequence) XM_005201715.3 , NM_001114189.1
    Accession Version XM_005201715.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sucrase-isomaltase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103818.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAGTCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201772.1
    CDS98..5536
    Translation

    Target ORF information:

    RefSeq Version XM_005201715.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sucrase-isomaltase (alpha-glucosidase) (SI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201715.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC AGAAATTTTC TGGATTAGAA ATTACTCTGA TTGTCCTCTT TGCTATAGTT 
    ACAATAATAG CTATTGCCCT AATTGTTGTT TTAGCAACTA AGACACCTGC TGTTGAAGAA
    ATCAGTGATT CAACCCGAGG CACATGCCCA AGTGTACTGA ACGATCCTAT CAACCACAGA
    ATAAACTGCA TTCCAGAACG ATCTCCCACA CAGACACTTT GTGCAGCTCG AGGTTGCTGC
    TGGAGGCCAT GGAATGATTC TGTTATTCCT TGGTGCTTCT TCGTAAATAA CCACGGCTAT
    AATGCTGAGA AAGTAACATC AACAAATGCT GGAGTTGAAG CCAAATTAAA AAGGAAAGCT
    TCACCTACAC TCTTTGGAAA TGACATTAAC AGCGTTCTCC TCACAACTCA AAGTCAGACA
    CGCAATCGTG TGCGGTTCAA GATCACTGAT CCAAATAATA GAAGATATGA AGTTCCTCAT
    CAGTTTGTAA AAGAATTTAG TGGAACTGCA GCTTCTGATC CACTGTATAA TGTGGAGGTT
    ATACATGATC CATTTAGCAT TAAAATTTCT AGAAGAAGCA ATAGTAAAAT TTTGTTTGAC
    ACCAGCATTG GTCCCCTGGT ATACTCTGAC CAGTATTTAC AGATCTCAGC CAGACTTCCC
    AATGAATACA TTTATGGTCT CGGGGAACAT ATTCATAAGA GATTTCGCCA TGATTTATAT
    TGGAAAACAT GGCCAATTTT TACTCGGGAT CAACTTCCTG GTGATAATAA TAATAATTTA
    TATGGCCATC AAACATTCTT CATGTGCATT GAGGATACTT CTGGAAAGTC ATTTGGTGTT
    TTCTTAATGA ACAGCAATGC AATGGAGATT TTCATCCAAC CTACTCCAAT AGTAACTTAC
    CGAGTTATAG GTGGAATTCT GGACTTTTAT ATCTTTCTGG GAGACACACC AGAACAAGTA
    GTTCAACAGT ATCAAGAGCT CATTGGACGA CCAGCAATGC CAGCTTATTG GAATCTTGGA
    TTTCAGCTGA GTCGCTGGAA TTACAAGACA CTTGATGTAG TGAAAGCAGT GGTACAAAGA
    AATCGAGATG CTGGCATACC ATTTGATACA CAAATCACTG ATATTGACTA TATGGAAGAC
    AAGAAAATCT TTACTTATGA TCAAGTTACC TTTAACGGCC TCCCAGAATT TGTTCAAGAT
    TTGCATGCTC ATGGGCAGAA ATACGTCATC ATCTTGGATC CTGCAGTTTC CATAGAGAAA
    CGTGCGAATG GGGCAGCATA TGAATCCTAT GATAGGGGAA CTGCCCAAAA GGTGTGGGTA
    AATGAGTCAG ATGGGACTAC AGCAATTATT GGAGAGGTAT GGCCAGGATT AACAGTATAC
    CCTGATTTCA CAAATCCAAG CTGCATAGAA TGGTGGGCAA ATGAATGCTA CCTTTTTCAT
    CAACAAGTAA ATTATGATGG ACTTTGGATT GACATGAATG AAGTTTCCAG CTTTGTTCAA
    GGTTCAAAGA AAGGATGCAG TGCAAACAAC CTGAATTACC CACCTTTTAC TCCCGATATT
    CTTGACAAAC TCATGTATTC CAAAACAATT TGCATGGATG CTATGCAATA CTGGGGAAAA
    CAGTATGATG TACACAGCCT CTATGGATAT AGTATGTCTA TAGCTACAGA GAAAGCTATA
    GAAAAAGTTC TTCCTAATAA AAGAAGCTTT ATTCTTACCC GATCAACTTT TGCTGGATCT
    GGAAGCCATT CTGCACATTG GTTAGGAGAC AATACTGCTT CTTGGGAACA AATGGAATGG
    TCTATTACAG GAATGCTGGA GTTTGGTTTG TTTGGAATAC CTTTGGTTGG AGCAGATATC
    TGTGGGTTTG TGGCTGAGAC CACAGAAGAG CTTTGCAGAA GGTGGATGCA ACTTGGAGCT
    TTTTATCCGT ATGCCAGAAA CCATAACGCT GATGGATATG AGCATCAGGA TCCAGCATTT
    TTTGGAAAGG ATTCTCTTTT GGTTAAATCA TCAAAACACT ATTTGAATAT TCGCTATGCC
    TTGTTACCTT TCCTTTATAC TCTATTTTAT AAAGCCCATA TGTTTGGAGA AACAGTAGCA
    AGGCCTTTTC TTCATGAGTT TTATGAGGAT ACTAATAGCT GGATTGAAGA TACTCAGTTC
    TTATGGGGCC CCTCATTACT TATAACCCCT GTATTGAGAC AGGGTGCAGA AACAGTGAGT
    GCATACATCC CTGATGCCAC TTGGTATGAC TTTGAAACTG GTGTAAAAAA GCCATGGAGA
    AAACAACGTG TTGATATGCA GCTTCCAGCA GACAAAATAG GATTACATCT TAGAGGCGGT
    TACATTATCC CCTTTCAACA ACCTGCTGTA ACTACAACAG CAAGCCGAAA GAACCCTCTA
    GGACTTATAA TTGCATTAAA TGATAATAAC ACAGCAAAAG GAGACTTTTT CTGGGATGAT
    GGAGAAACTA AAGATACCAT CCAAAAGGGC AACTACATTT TATATACATT TTCAGTTTCT
    AATAACAAGC TAGATATCAC ATGTATGCAT TCATCGTACC CAGAAGGAAC TGCTTTAGCA
    TTTGAGACTA TAAAAGTCCT TGGGTTGATA AACACTGTTA CAGAAGTTTC AGTGATAGAA
    GATAATCAAC CATTGAGAGC TCATTACAAT TTCAGTTATG ATGCTTCCAA CCAGATTCTA
    CTAATTCACA ATCTGACATT TAACCTTGGA GGAAACTTTA CTGTTCAATG GGATCAAACT
    TTCTTGGAAG TTGAAAAGTT TACTTGTTAT CCAGATTCAA ACACTGCAAC TAAAGAACTC
    TGCGAAGAAC GTGGCTGCTT ATGGCAAACG GTTCATTTAG GATCCAAAGC ACCTGAGTGT
    TACTTTCCTA AAGAAGACAA CTCTTATTTA GTCCACTCAA CTCAGTACTC ATCAGTGGGT
    ATAACAGCTG ACCTCCAGCT GAATACTGCA AATCGAATAA AGCTACCTTC TGATTCCATT
    TCGTCTCTTC GTGTGGAGGT GAAATATCAC AAAGATGATA TGTTGCAGTT TAAGATTTAT
    GATGCCCAAA ATAAGAGATA TGAAGTTCCA GTACCATTGA ACATTCCAAA TACACCAACA
    AGTACTTATG AAACTAGACT TTATGATGTT GAAATCAAGG AAAATCCTTT TGGCATTCAG
    ATTCGAAGAA GAAGCACAAG AAGAGTTATT TGGGATTCTC GCCTTCCTGG TTTTGCTTTT
    AACAACCAGT TCATTCAGAT ATCTACCCGC CTGCCTTCAG AATATGTGTA TGGTTTTGGG
    GAAATGGAAC ACACAACATT TAAGCGTGAC CTGAACTGGC ATACTTGGGG AATGTTCACA
    AGAGACCAAC CCCCTGGTTA CAAACTTAAT TCCTATGGAT TTCATCCCTA TTACATGGCT
    CTGGAAGATG AGAACAATGC TCATGGAGTT CTCTTACTTA ACAGCAATGC AATGGATGTT
    ACATTTCAGC CAATGCCTGC TCTGACATAT CGCATGATTG GAGGAATCTT GGACTTTTAT
    ATGTTTTTGG GCCCATCTCC AGAAGTCACA ACAAAGCAAT ACCATGAAGT AATCGGCCAA
    CCAGTCATGC CACCTTATTG GGCTTTGGGA TTCCAGTTAT GTCGTTATGG ATACAGAAAT
    ACTTCACAAG TTGAAGAAGT ATATAATGAC ATGGTGGCTG CCCAGATCCC CTATGATGTT
    CAGTACACAG ATATTGATTA CATGGAAAGG CAACTAGACT TTACAATTGA TGATGAATTT
    CATGACCTTC CTCAGTTTGT TGACAAAATA AGAAGTGAGG GAATGAGATA CATTATTATC
    CTGGATCCAG CAATTTCAGG AAATGAAACG AAGCCTTACC CTGCCTTTGA AAGAGGACAG
    GAGAAAGATG TCTTTGTCAA ATGGCCTAAC ACAAGTGATA TTTGTTGGGC AAAGGTTAGG
    CCAGATTTGC CTAATGTGAC AATAGATCAA AGTCTAACTG AAGATGAAGC TGCTAATGCT
    ACCAAAGCTC ATGCAGCTTT TCCAGATTTC TTCAGGAATT CCACAGCAGA GTGGTGGGCA
    AGGGAAATTA TAGATTTTTA TAATAACCAG ATGAAGTTTG ATGGATTGTG GATTGATATG
    AATGAACCAT CAAGTTTTGT AAATGGAACA ACTACTAATC AATGCAGAAA TGCAGAGCTG
    AATTATCCAC CTTATTTCCC AGAACTCACA AAAAGAACTG ATGGATTACA TTTCAGAACC
    GTGTGTATGG AAACAGAGCA GATTCTTAGT GATGGATCAT CTGTTTTGCA TTATGATGTT
    CACAATCTAT ATGGATGGTC ACAGCTGAAA CCAACTTATG ATGCATTGCA AAAGGCAACT
    GGCAAAAGAG GAATTATTAT TTCTCGTTCC ACATATCCCT CTGCTGGACG ATGGTCAGGA
    CACTGGCTTG GAGACAACTA TGCAACATGG GACAATTTGG AAAAATCTAT CATTGGTATG
    ATGGAATTTA GTCTCTTTGG AATGTCATAT ACTGGAGCAG ATATCTGTGG TTTCTTCAAC
    AATTCAGAAT ATCAGCTTTG TGCCCGCTGG ATGCAACTTG GAGCATTTTA TCCATATGCA
    AGAAATCACA ACAGTGCATT TACTAGAAGA CAAGATCCTG CTTCCTGGAA TAAAACTTTT
    TCTGCAATGT CAAGGGATAT TCTAAATATT AGATACACTT TATTGCCCTA CTTCTATACA
    CAGATGTATG AAATTCATGC TCATGGTGGC ACTGTTATCC GACCCCTTTT GCATGAGTTT
    TTAAATGAAA AACCAACCTG GGATATATTC AAACAGTTTC TGTGGGGTCC AGCATTTATG
    GTTACTCCTG TATTGGAACC TTACGTTGAC ACTGTAGAAG GCTATGTCCC TAATGCTCGG
    TGGTTTGATT ACCATACAGG CAAAGATATT GGTGTCAGAG AAACATTTCA TACATTTGCA
    GCTCCTTTAT ATGAAATAAA CCTACATGTC CGTGGTGGTT ACATCCTACC ATGTCAAGAG
    CCTGCTAACA ATACATTTTA CAGCCGACAG AATTACATGA AGCTCATTGT TGCTGCAGAT
    GATAATCAGA TGGCACAAGG ATCTCTGTTT TGGGATGATG GAGAGACTAT TGACACCTAT
    GAAAGAGACC TATATTCCTT GGTACAATTT AATTTAAATA AGACCATCTT GACGAGCACT
    ATACTGAAAA ATGGTTACAT AAACAGAAAT GAAATGAGGC TTGGATATAT CCACATATGG
    GGGAAAGAAA AGATGCGTGT TAGTGAGGTT ACTCTCACAC ACAACGGAAA TAAAGAGTCT
    GTTAATTTTA CTGAAGACCC AAACCTGGAG ATATTAGACA TTGATCTAAC AGGGAATAAT
    GTTACGCTAG ATGAACCAAT AGAAGTCAAC TGGTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.