GRB14 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRB14
Entrez Gene ID	497029
Full Name	growth factor receptor bound protein 14
General protein information	Preferred Names growth factor receptor bound protein 14 Names growth factor receptor-bound protein 14 GRB14 adapter protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475191.1	XP_015330677.1	growth factor receptor-bound protein 14 isoform X1
XM_024999498.1	XP_024855266.1	growth factor receptor-bound protein 14 isoform X1
XM_024999504.1	XP_024855272.1	growth factor receptor-bound protein 14 isoform X2
XM_010801965.3	XP_010800267.1	growth factor receptor-bound protein 14 isoform X4
NM_001011681.3	NP_001011681.2	growth factor receptor-bound protein 14
XM_010801964.2	XP_010800266.1	growth factor receptor-bound protein 14 isoform X2
XM_010801964.3	XP_010800266.1	growth factor receptor-bound protein 14 isoform X3
XM_010801965.2	XP_010800267.1	growth factor receptor-bound protein 14 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Grb14	NP_113811.1
Danio rerio (zebrafish)	LOC100334415	XP_002661994.2
Xenopus tropicalis (tropical clawed frog)	grb14	XP_004917759.1
Bos taurus (cattle)	GRB14	NP_001011681.2
Gallus gallus (chicken)	GRB14	XP_422029.4
Homo sapiens (human)	GRB14	NP_004481.2
Pan troglodytes (chimpanzee)	GRB14	XP_515864.2
Mus musculus (house mouse)	Grb14	NP_057928.1
	LOC707075	XP_002808050.1
Canis lupus familiaris (dog)	GRB14	XP_005640314.1

Gene Ontology
Bibliography

Related articles in PubMed

Grb14 mRNA levels during follicular deviation in cattle are higher in granulosa cells of subordinate compared to dominant follicles.
Bohrer RC, Rosa PR, Ferreira R, Bordignon V, Oliveira JF, Gon?alves PB
Reproduction in domestic animals = Zuchthygiene48(3)396-401(2013 Jun)

Growth factor receptor-bound protein 14: a new modulator of photoreceptor-specific cyclic-nucleotide-gated channel.
Gupta VK, Rajala A, Daly RJ, Rajala RV
EMBO reports11(11)861-7(2010 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Lipid-protein interactions of growth factor receptor-bound protein 14 in insulin receptor signaling.
Rajala RV, Chan MD, Rajala A
Biochemistry44(47)15461-71(2005 Nov)

Identification of a NPXY motif in growth factor receptor-bound protein 14 (Grb14) and its interaction with the phosphotyrosine-binding (PTB) domain of IRS-1.
Rajala RV, Chan MD
Biochemistry44(22)7929-35(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that Grb14 may play a regulatory role in granulosa cells during follicular deviation in cattle.
Title: Grb14 mRNA levels during follicular deviation in cattle are higher in granulosa cells of subordinate compared to dominant follicles.

Grb14 contains a phosphorylated insulin receptor interacting domain between the pleckstrin homolog) and SH2 domains that binds to and forms a specific complex with the cytoplasmic domain of IRbeta
Title: Lipid-protein interactions of growth factor receptor-bound protein 14 in insulin receptor signaling.

The growth factor receptor bound protein (Grb) 14 NPXY motif could be acting as a dominant negative for insulin receptor substrate (IRS)-1 functions in the retina.
Title: Identification of a NPXY motif in growth factor receptor-bound protein 14 (Grb14) and its interaction with the phosphotyrosine-binding (PTB) domain of IRS-1.

The following GRB14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRB14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa45853	XM_010801965.3 Latest version!	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa227194	XM_024999498.1 Latest version!	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa227195	XM_024999504.1 Latest version!	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa44082	NM_001011681.3 Latest version!	Bos taurus growth factor receptor bound protein 14 (GRB14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa45852	XM_010801964.3 Latest version!	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa87909	XM_015475191.1 Latest version!	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OBa45852	XM_010801964.2	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa45853	XM_010801965.2	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRB14 ( XM_010801965.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800267.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa45853
Clone ID Related Accession (Same CDS sequence)	XM_010801965.3 , XM_010801965.2
Accession Version	XM_010801965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGA GTGCAAGAAG GGTCACTCTG CCCGCAATAA CGCCCATAGT TCTCCAGAAA 
AGGGTAATTA AAGTATACAG TGAAGATGAA ACCAGCAGAG CTTTAGAGGT ACCCAGTGAC 
ATAACTGCCC GAGATGTTTG CCAGCTGTTG ATCCTGAAGA ATCATTACAT TGATGACCAC 
AGCTGGACCC TTTTTGAGCA CCTGCCTCAT GTAGGTCTAG AAAGAACAAT AGAAGACCAC 
GAGCTGGTGA TTGAAGTGCT ATCTAACTGG GGAATGGAAG AAGAAAATAA GCTATACTTT 
AGAAAAAATT ATGCCAAATA TGAATTCTTT AAAAACCCAA TGTATTTTTT TCCAGAGCAT 
ATGGTGTCTT TTGCAACTGA AACCAATGGT GAAATATCCC CCACACAGAT TTTGCAGATG 
TTTCTAAGTT CAAGCACATA TCCTGAAATC CATGGCTTCT TACATGCAAA AGAACAGGGA 
AAGAAGTCCT GGAAAAAGAT TTATTTCCTT TTAAGAAGAT CCGGTTTATA TTTTTCTACT 
AAAGGGACAT CAAAGGAACC ACGGCATTTG CAGTTTTTCA GCGAATTTGG CAATAGTGAT 
ATTTATGTGT CACTGGCAGG CAAAAAAAAA CACGGAGCAC CGACTAACTA TGGATTCTGC 
TTTAAGCCTA ACAAAGCGGG AGGGCCCCGA GACCTGAAAA TGCTCTGTGC AGAAGAAGAG 
CAGAGTAGGA CGTGCTGGGT GACCGCGATT AGATTGCTTA AGTATGGCAT GCAGCTGTAC 
CAGAATTACA TGCATCCGTA TCAAGGCAGA AGTGGCTACA GTTCTCAGAG TATATCTCCC 
ATGAGAAGTA TATCAGAGAA TTCCCTGGTA GCAATGGATT TCTCAGGCCA GAAAACCAGA 
GTTATAGAAA ATCCCACTGA AGCCCTTTCC GTTGCAGTTG AAGAAGGACT AGCTTGGAGG 
AAAAAAGGAT GTTTACGCCT GGGCAGTCAC GGTAGCCCCA CTGCATCCTC ACAGAGCTCT 
GCCACAAGCA TGGCTATCCA CCGGTCCCAA CCATGGTTTC ACCACAAAAT TTCTAGAGAG 
GAAGCTCAGC GATTGATTAT TCAGCAAGGT CTAGTGGATG GAGTTTTCTT GGTACGGGAT 
AGTCAGAGTA ACCCCAAAAC TTTTGTACTG TCAATGAGTC ATGGACAAAA AATAAAGCAC 
TTTCAAATTA TACCGGTGGA AGATGATGGT GAGATGTTCC ACACCCTAGA TGATGGACAT 
ACAAGATTTA CGGATCTAAT CCAGTTGGTG GAGTTCTATC AGCTCAATAA GGGTGTTCTT 
CCTTGCAAGC TGAAACATTA TTGTGCTAGG ATTGCACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800267.1
CDS	214..1575
Translation	MSLSARRVTLPAITPIVLQKRVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGYSSQSISPMRSISENSLVAMDFSGQKTRVIENPTEALSVAVEEGLAWRKKGCLRLGSHGSPTASSQSSATSMAIHRSQPWFHHKISREEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Target ORF information:

RefSeq Version	XM_010801965.3
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801965.3

ORF Insert Sequence:

ATGAGTCTGA GTGCAAGAAG GGTCACTCTG CCCGCAATAA CGCCCATAGT TCTCCAGAAA 
AGGGTAATTA AAGTATACAG TGAAGATGAA ACCAGCAGAG CTTTAGAGGT ACCCAGTGAC 
ATAACTGCCC GAGATGTTTG CCAGCTGTTG ATCCTGAAGA ATCATTACAT TGATGACCAC 
AGCTGGACCC TTTTTGAGCA CCTGCCTCAT GTAGGTCTAG AAAGAACAAT AGAAGACCAC 
GAGCTGGTGA TTGAAGTGCT ATCTAACTGG GGAATGGAAG AAGAAAATAA GCTATACTTT 
AGAAAAAATT ATGCCAAATA TGAATTCTTT AAAAACCCAA TGTATTTTTT TCCAGAGCAT 
ATGGTGTCTT TTGCAACTGA AACCAATGGT GAAATATCCC CCACACAGAT TTTGCAGATG 
TTTCTAAGTT CAAGCACATA TCCTGAAATC CATGGCTTCT TACATGCAAA AGAACAGGGA 
AAGAAGTCCT GGAAAAAGAT TTATTTCCTT TTAAGAAGAT CCGGTTTATA TTTTTCTACT 
AAAGGGACAT CAAAGGAACC ACGGCATTTG CAGTTTTTCA GCGAATTTGG CAATAGTGAT 
ATTTATGTGT CACTGGCAGG CAAAAAAAAA CACGGAGCAC CGACTAACTA TGGATTCTGC 
TTTAAGCCTA ACAAAGCGGG AGGGCCCCGA GACCTGAAAA TGCTCTGTGC AGAAGAAGAG 
CAGAGTAGGA CGTGCTGGGT GACCGCGATT AGATTGCTTA AGTATGGCAT GCAGCTGTAC 
CAGAATTACA TGCATCCGTA TCAAGGCAGA AGTGGCTACA GTTCTCAGAG TATATCTCCC 
ATGAGAAGTA TATCAGAGAA TTCCCTGGTA GCAATGGATT TCTCAGGCCA GAAAACCAGA 
GTTATAGAAA ATCCCACTGA AGCCCTTTCC GTTGCAGTTG AAGAAGGACT AGCTTGGAGG 
AAAAAAGGAT GTTTACGCCT GGGCAGTCAC GGTAGCCCCA CTGCATCCTC ACAGAGCTCT 
GCCACAAGCA TGGCTATCCA CCGGTCCCAA CCATGGTTTC ACCACAAAAT TTCTAGAGAG 
GAAGCTCAGC GATTGATTAT TCAGCAAGGT CTAGTGGATG GAGTTTTCTT GGTACGGGAT 
AGTCAGAGTA ACCCCAAAAC TTTTGTACTG TCAATGAGTC ATGGACAAAA AATAAAGCAC 
TTTCAAATTA TACCGGTGGA AGATGATGGT GAGATGTTCC ACACCCTAGA TGATGGACAT 
ACAAGATTTA CGGATCTAAT CCAGTTGGTG GAGTTCTATC AGCTCAATAA GGGTGTTCTT 
CCTTGCAAGC TGAAACATTA TTGTGCTAGG ATTGCACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( XM_010801965.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800267.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45853
Clone ID Related Accession (Same CDS sequence)	XM_010801965.3 , XM_010801965.2
Accession Version	XM_010801965.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGA GTGCAAGAAG GGTCACTCTG CCCGCAATAA CGCCCATAGT TCTCCAGAAA 
AGGGTAATTA AAGTATACAG TGAAGATGAA ACCAGCAGAG CTTTAGAGGT ACCCAGTGAC 
ATAACTGCCC GAGATGTTTG CCAGCTGTTG ATCCTGAAGA ATCATTACAT TGATGACCAC 
AGCTGGACCC TTTTTGAGCA CCTGCCTCAT GTAGGTCTAG AAAGAACAAT AGAAGACCAC 
GAGCTGGTGA TTGAAGTGCT ATCTAACTGG GGAATGGAAG AAGAAAATAA GCTATACTTT 
AGAAAAAATT ATGCCAAATA TGAATTCTTT AAAAACCCAA TGTATTTTTT TCCAGAGCAT 
ATGGTGTCTT TTGCAACTGA AACCAATGGT GAAATATCCC CCACACAGAT TTTGCAGATG 
TTTCTAAGTT CAAGCACATA TCCTGAAATC CATGGCTTCT TACATGCAAA AGAACAGGGA 
AAGAAGTCCT GGAAAAAGAT TTATTTCCTT TTAAGAAGAT CCGGTTTATA TTTTTCTACT 
AAAGGGACAT CAAAGGAACC ACGGCATTTG CAGTTTTTCA GCGAATTTGG CAATAGTGAT 
ATTTATGTGT CACTGGCAGG CAAAAAAAAA CACGGAGCAC CGACTAACTA TGGATTCTGC 
TTTAAGCCTA ACAAAGCGGG AGGGCCCCGA GACCTGAAAA TGCTCTGTGC AGAAGAAGAG 
CAGAGTAGGA CGTGCTGGGT GACCGCGATT AGATTGCTTA AGTATGGCAT GCAGCTGTAC 
CAGAATTACA TGCATCCGTA TCAAGGCAGA AGTGGCTACA GTTCTCAGAG TATATCTCCC 
ATGAGAAGTA TATCAGAGAA TTCCCTGGTA GCAATGGATT TCTCAGGCCA GAAAACCAGA 
GTTATAGAAA ATCCCACTGA AGCCCTTTCC GTTGCAGTTG AAGAAGGACT AGCTTGGAGG 
AAAAAAGGAT GTTTACGCCT GGGCAGTCAC GGTAGCCCCA CTGCATCCTC ACAGAGCTCT 
GCCACAAGCA TGGCTATCCA CCGGTCCCAA CCATGGTTTC ACCACAAAAT TTCTAGAGAG 
GAAGCTCAGC GATTGATTAT TCAGCAAGGT CTAGTGGATG GAGTTTTCTT GGTACGGGAT 
AGTCAGAGTA ACCCCAAAAC TTTTGTACTG TCAATGAGTC ATGGACAAAA AATAAAGCAC 
TTTCAAATTA TACCGGTGGA AGATGATGGT GAGATGTTCC ACACCCTAGA TGATGGACAT 
ACAAGATTTA CGGATCTAAT CCAGTTGGTG GAGTTCTATC AGCTCAATAA GGGTGTTCTT 
CCTTGCAAGC TGAAACATTA TTGTGCTAGG ATTGCACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800267.1
CDS	154..1515
Translation	MSLSARRVTLPAITPIVLQKRVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGYSSQSISPMRSISENSLVAMDFSGQKTRVIENPTEALSVAVEEGLAWRKKGCLRLGSHGSPTASSQSSATSMAIHRSQPWFHHKISREEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Target ORF information:

RefSeq Version	XM_010801965.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801965.2

ORF Insert Sequence:

ATGAGTCTGA GTGCAAGAAG GGTCACTCTG CCCGCAATAA CGCCCATAGT TCTCCAGAAA 
AGGGTAATTA AAGTATACAG TGAAGATGAA ACCAGCAGAG CTTTAGAGGT ACCCAGTGAC 
ATAACTGCCC GAGATGTTTG CCAGCTGTTG ATCCTGAAGA ATCATTACAT TGATGACCAC 
AGCTGGACCC TTTTTGAGCA CCTGCCTCAT GTAGGTCTAG AAAGAACAAT AGAAGACCAC 
GAGCTGGTGA TTGAAGTGCT ATCTAACTGG GGAATGGAAG AAGAAAATAA GCTATACTTT 
AGAAAAAATT ATGCCAAATA TGAATTCTTT AAAAACCCAA TGTATTTTTT TCCAGAGCAT 
ATGGTGTCTT TTGCAACTGA AACCAATGGT GAAATATCCC CCACACAGAT TTTGCAGATG 
TTTCTAAGTT CAAGCACATA TCCTGAAATC CATGGCTTCT TACATGCAAA AGAACAGGGA 
AAGAAGTCCT GGAAAAAGAT TTATTTCCTT TTAAGAAGAT CCGGTTTATA TTTTTCTACT 
AAAGGGACAT CAAAGGAACC ACGGCATTTG CAGTTTTTCA GCGAATTTGG CAATAGTGAT 
ATTTATGTGT CACTGGCAGG CAAAAAAAAA CACGGAGCAC CGACTAACTA TGGATTCTGC 
TTTAAGCCTA ACAAAGCGGG AGGGCCCCGA GACCTGAAAA TGCTCTGTGC AGAAGAAGAG 
CAGAGTAGGA CGTGCTGGGT GACCGCGATT AGATTGCTTA AGTATGGCAT GCAGCTGTAC 
CAGAATTACA TGCATCCGTA TCAAGGCAGA AGTGGCTACA GTTCTCAGAG TATATCTCCC 
ATGAGAAGTA TATCAGAGAA TTCCCTGGTA GCAATGGATT TCTCAGGCCA GAAAACCAGA 
GTTATAGAAA ATCCCACTGA AGCCCTTTCC GTTGCAGTTG AAGAAGGACT AGCTTGGAGG 
AAAAAAGGAT GTTTACGCCT GGGCAGTCAC GGTAGCCCCA CTGCATCCTC ACAGAGCTCT 
GCCACAAGCA TGGCTATCCA CCGGTCCCAA CCATGGTTTC ACCACAAAAT TTCTAGAGAG 
GAAGCTCAGC GATTGATTAT TCAGCAAGGT CTAGTGGATG GAGTTTTCTT GGTACGGGAT 
AGTCAGAGTA ACCCCAAAAC TTTTGTACTG TCAATGAGTC ATGGACAAAA AATAAAGCAC 
TTTCAAATTA TACCGGTGGA AGATGATGGT GAGATGTTCC ACACCCTAGA TGATGGACAT 
ACAAGATTTA CGGATCTAAT CCAGTTGGTG GAGTTCTATC AGCTCAATAA GGGTGTTCTT 
CCTTGCAAGC TGAAACATTA TTGTGCTAGG ATTGCACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( XM_024999498.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855266.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa227194
Clone ID Related Accession (Same CDS sequence)	XM_024999498.1
Accession Version	XM_024999498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGATGTTTC TAAGTTCAAG CACATATCCT GAAATCCATG GCTTCTTACA TGCAAAAGAA 
CAGGGAAAGA AGTCCTGGAA AAAGATTTAT TTCCTTTTAA GAAGATCCGG TTTATATTTT 
TCTACTAAAG GGACATCAAA GGAACCACGG CATTTGCAGT TTTTCAGCGA ATTTGGCAAT 
AGTGATATTT ATGTGTCACT GGCAGGCAAA AAAAAACACG GAGCACCGAC TAACTATGGA 
TTCTGCTTTA AGCCTAACAA AGCGGGAGGG CCCCGAGACC TGAAAATGCT CTGTGCAGAA 
GAAGAGCAGA GTAGGACGTG CTGGGTGACC GCGATTAGAT TGCTTAAGTA TGGCATGCAG 
CTGTACCAGA ATTACATGCA TCCGTATCAA GGCAGAAGTG GCTACAGTTC TCAGAGTATA 
TCTCCCATGA GAAGTATATC AGAGAATTCC CTGGTAGCAA TGGATTTCTC AGGCCAGAAA 
ACCAGAGTTA TAGAAAATCC CACTGAAGCC CTTTCCGTTG CAGTTGAAGA AGGACTAGCT 
TGGAGGAAAA AAGGATGTTT ACGCCTGGGC AGTCACGGTA GCCCCACTGC ATCCTCACAG 
AGCTCTGCCA CAAGCATGGC TATCCACCGG TCCCAACCAT GGTTTCACCA CAAAATTTCT 
AGAGAGGAAG CTCAGCGATT GATTATTCAG CAAGGTCTAG TGGATGGAGT TTTCTTGGTA 
CGGGATAGTC AGAGTAACCC CAAAACTTTT GTACTGTCAA TGAGTCATGG ACAAAAAATA 
AAGCACTTTC AAATTATACC GGTGGAAGAT GATGGTGAGA TGTTCCACAC CCTAGATGAT 
GGACATACAA GATTTACGGA TCTAATCCAG TTGGTGGAGT TCTATCAGCT CAATAAGGGT 
GTTCTTCCTT GCAAGCTGAA ACATTATTGT GCTAGGATTG CACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855266.1
CDS	137..1684
Translation	MTTSLQDGQSAAGRAAARDSPLAAQVCGAAQGRGDARDLAPAPWLHARALLPPPDATRGCAADRRKKKDLDVLEMPSIPNPFPELCCSPFTSVLSAGLFPKANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGYSSQSISPMRSISENSLVAMDFSGQKTRVIENPTEALSVAVEEGLAWRKKGCLRLGSHGSPTASSQSSATSMAIHRSQPWFHHKISREEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Target ORF information:

RefSeq Version	XM_024999498.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999498.1

ORF Insert Sequence:

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGATGTTTC TAAGTTCAAG CACATATCCT GAAATCCATG GCTTCTTACA TGCAAAAGAA 
CAGGGAAAGA AGTCCTGGAA AAAGATTTAT TTCCTTTTAA GAAGATCCGG TTTATATTTT 
TCTACTAAAG GGACATCAAA GGAACCACGG CATTTGCAGT TTTTCAGCGA ATTTGGCAAT 
AGTGATATTT ATGTGTCACT GGCAGGCAAA AAAAAACACG GAGCACCGAC TAACTATGGA 
TTCTGCTTTA AGCCTAACAA AGCGGGAGGG CCCCGAGACC TGAAAATGCT CTGTGCAGAA 
GAAGAGCAGA GTAGGACGTG CTGGGTGACC GCGATTAGAT TGCTTAAGTA TGGCATGCAG 
CTGTACCAGA ATTACATGCA TCCGTATCAA GGCAGAAGTG GCTACAGTTC TCAGAGTATA 
TCTCCCATGA GAAGTATATC AGAGAATTCC CTGGTAGCAA TGGATTTCTC AGGCCAGAAA 
ACCAGAGTTA TAGAAAATCC CACTGAAGCC CTTTCCGTTG CAGTTGAAGA AGGACTAGCT 
TGGAGGAAAA AAGGATGTTT ACGCCTGGGC AGTCACGGTA GCCCCACTGC ATCCTCACAG 
AGCTCTGCCA CAAGCATGGC TATCCACCGG TCCCAACCAT GGTTTCACCA CAAAATTTCT 
AGAGAGGAAG CTCAGCGATT GATTATTCAG CAAGGTCTAG TGGATGGAGT TTTCTTGGTA 
CGGGATAGTC AGAGTAACCC CAAAACTTTT GTACTGTCAA TGAGTCATGG ACAAAAAATA 
AAGCACTTTC AAATTATACC GGTGGAAGAT GATGGTGAGA TGTTCCACAC CCTAGATGAT 
GGACATACAA GATTTACGGA TCTAATCCAG TTGGTGGAGT TCTATCAGCT CAATAAGGGT 
GTTCTTCCTT GCAAGCTGAA ACATTATTGT GCTAGGATTG CACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( XM_024999504.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855272.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X2, mRNA.

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CloneID	OBa227195
Clone ID Related Accession (Same CDS sequence)	XM_024999504.1
Accession Version	XM_024999504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGTAT GGCATGCAGC TGTACCAGAA TTACATGCAT 
CCGTATCAAG GCAGAAGTGG CTACAGTTCT CAGAGTATAT CTCCCATGAG AAGTATATCA 
GAGAATTCCC TGGTAGCAAT GGATTTCTCA GGCCAGAAAA CCAGAGTTAT AGAAAATCCC 
ACTGAAGCCC TTTCCGTTGC AGTTGAAGAA GGACTAGCTT GGAGGAAAAA AGGATGTTTA 
CGCCTGGGCA GTCACGGTAG CCCCACTGCA TCCTCACAGA GCTCTGCCAC AAGCATGGCT 
ATCCACCGGT CCCAACCATG GTTTCACCAC AAAATTTCTA GAGAGGAAGC TCAGCGATTG 
ATTATTCAGC AAGGTCTAGT GGATGGAGTT TTCTTGGTAC GGGATAGTCA GAGTAACCCC 
AAAACTTTTG TACTGTCAAT GAGTCATGGA CAAAAAATAA AGCACTTTCA AATTATACCG 
GTGGAAGATG ATGGTGAGAT GTTCCACACC CTAGATGATG GACATACAAG ATTTACGGAT 
CTAATCCAGT TGGTGGAGTT CTATCAGCTC AATAAGGGTG TTCTTCCTTG CAAGCTGAAA 
CATTATTGTG CTAGGATTGC ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855272.1
CDS	135..1661
Translation	MTTSLQDGQSAAGRAAARDSPLAAQVCGAAQGRGDARDLAPAPWLHARALLPPPDATRGCAADRRKKKDLDVLEMPSIPNPFPELCCSPFTSVLSAGLFPKANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKYGMQLYQNYMHPYQGRSGYSSQSISPMRSISENSLVAMDFSGQKTRVIENPTEALSVAVEEGLAWRKKGCLRLGSHGSPTASSQSSATSMAIHRSQPWFHHKISREEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Target ORF information:

RefSeq Version	XM_024999504.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999504.1

ORF Insert Sequence:

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGTAT GGCATGCAGC TGTACCAGAA TTACATGCAT 
CCGTATCAAG GCAGAAGTGG CTACAGTTCT CAGAGTATAT CTCCCATGAG AAGTATATCA 
GAGAATTCCC TGGTAGCAAT GGATTTCTCA GGCCAGAAAA CCAGAGTTAT AGAAAATCCC 
ACTGAAGCCC TTTCCGTTGC AGTTGAAGAA GGACTAGCTT GGAGGAAAAA AGGATGTTTA 
CGCCTGGGCA GTCACGGTAG CCCCACTGCA TCCTCACAGA GCTCTGCCAC AAGCATGGCT 
ATCCACCGGT CCCAACCATG GTTTCACCAC AAAATTTCTA GAGAGGAAGC TCAGCGATTG 
ATTATTCAGC AAGGTCTAGT GGATGGAGTT TTCTTGGTAC GGGATAGTCA GAGTAACCCC 
AAAACTTTTG TACTGTCAAT GAGTCATGGA CAAAAAATAA AGCACTTTCA AATTATACCG 
GTGGAAGATG ATGGTGAGAT GTTCCACACC CTAGATGATG GACATACAAG ATTTACGGAT 
CTAATCCAGT TGGTGGAGTT CTATCAGCTC AATAAGGGTG TTCTTCCTTG CAAGCTGAAA 
CATTATTGTG CTAGGATTGC ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( NM_001011681.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001011681.2 Bos taurus growth factor receptor bound protein 14 (GRB14), mRNA.

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CloneID	OBa44082
Clone ID Related Accession (Same CDS sequence)	NM_001011681.3
Accession Version	NM_001011681.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY772475.1, AJ696563.1, DN286318.1 and BI976591.1. On Dec 23, 2008 this sequence version replaced NM_001011681.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGTATGGCA TGCAGCTGTA CCAGAATTAC ATGCATCCGT ATCAAGGCAG AAGTGGCTAC 
AGTTCTCAGA GTATATCTCC CATGAGAAGT ATATCAGAGA ATTCCCTGGT AGCAATGGAT 
TTCTCAGGCC AGAAAACCAG AGTTATAGAA AATCCCACTG AAGCCCTTTC CGTTGCAGTT 
GAAGAAGGAC TAGCTTGGAG GAAAAAAGGA TGTTTACGCC TGGGCAGTCA CGGTAGCCCC 
ACTGCATCCT CACAGAGCTC TGCCACAAGC ATGGCTATCC ACCGGTCCCA ACCATGGTTT 
CACCACAAAA TTTCTAGAGA GGAAGCTCAG CGATTGATTA TTCAGCAAGG TCTAGTGGAT 
GGAGTTTTCT TGGTACGGGA TAGTCAGAGT AACCCCAAAA CTTTTGTACT GTCAATGAGT 
CATGGACAAA AAATAAAGCA CTTTCAAATT ATACCGGTGG AAGATGATGG TGAGATGTTC 
CACACCCTAG ATGATGGACA TACAAGATTT ACGGATCTAA TCCAGTTGGT GGAGTTCTAT 
CAGCTCAATA AGGGTGTTCT TCCTTGCAAG CTGAAACATT ATTGTGCTAG GATTGCACTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011681.2
CDS	177..1799
Translation	MTTSLQDGQSAAGRAAARDSPLAAQVCGAAQGRGDARDLAPAPWLHARALLPPPDATRGCAADRRKKKDLDVLEMPSIPNPFPELCCSPFTSVLSAGLFPKANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGYSSQSISPMRSISENSLVAMDFSGQKTRVIENPTEALSVAVEEGLAWRKKGCLRLGSHGSPTASSQSSATSMAIHRSQPWFHHKISREEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Target ORF information:

RefSeq Version	NM_001011681.3
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011681.3

ORF Insert Sequence:

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGTATGGCA TGCAGCTGTA CCAGAATTAC ATGCATCCGT ATCAAGGCAG AAGTGGCTAC 
AGTTCTCAGA GTATATCTCC CATGAGAAGT ATATCAGAGA ATTCCCTGGT AGCAATGGAT 
TTCTCAGGCC AGAAAACCAG AGTTATAGAA AATCCCACTG AAGCCCTTTC CGTTGCAGTT 
GAAGAAGGAC TAGCTTGGAG GAAAAAAGGA TGTTTACGCC TGGGCAGTCA CGGTAGCCCC 
ACTGCATCCT CACAGAGCTC TGCCACAAGC ATGGCTATCC ACCGGTCCCA ACCATGGTTT 
CACCACAAAA TTTCTAGAGA GGAAGCTCAG CGATTGATTA TTCAGCAAGG TCTAGTGGAT 
GGAGTTTTCT TGGTACGGGA TAGTCAGAGT AACCCCAAAA CTTTTGTACT GTCAATGAGT 
CATGGACAAA AAATAAAGCA CTTTCAAATT ATACCGGTGG AAGATGATGG TGAGATGTTC 
CACACCCTAG ATGATGGACA TACAAGATTT ACGGATCTAA TCCAGTTGGT GGAGTTCTAT 
CAGCTCAATA AGGGTGTTCT TCCTTGCAAG CTGAAACATT ATTGTGCTAG GATTGCACTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( XM_010801964.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800266.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X2, mRNA.

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CloneID	OBa45852
Clone ID Related Accession (Same CDS sequence)	XM_010801964.2 , XM_010801964.3
Accession Version	XM_010801964.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGTATGGCA TGCAGCTGTA CCAGAATTAC ATGCATCCGT ATCAAGGCAG AAGTGGCTAC 
AGTTCTCAGA GTATATCTCC CATGTTTCAG GTCCTTGAAA AACATACCGC TCTCCAGGAA 
ACCCAAAAGA TGCAAGCCAA AATATTGGTT GTGCATTACA TTAAGACTAG CACCGCTTCA 
CAAGTTTATC CTAAATGGCA CTTGGATATT TATACACAGA TACATCTTTC CCCGTCTTTG 
GGACCTAATT TTAATTTCCT GTGCATTAAC AAGAAATTTA TTTTTGAACT TAAAGTTTAT 
GTTCTGCTGT TTTTGCTTGT GCTCTTTACT GGTGAATATA TTGGAAATTT GGACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800266.1
CDS	162..1541
Translation	MTTSLQDGQSAAGRAAARDSPLAAQVCGAAQGRGDARDLAPAPWLHARALLPPPDATRGCAADRRKKKDLDVLEMPSIPNPFPELCCSPFTSVLSAGLFPKANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGYSSQSISPMFQVLEKHTALQETQKMQAKILVVHYIKTSTASQVYPKWHLDIYTQIHLSPSLGPNFNFLCINKKFIFELKVYVLLFLLVLFTGEYIGNLDV

Target ORF information:

RefSeq Version	XM_010801964.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801964.2

ORF Insert Sequence:

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGTATGGCA TGCAGCTGTA CCAGAATTAC ATGCATCCGT ATCAAGGCAG AAGTGGCTAC 
AGTTCTCAGA GTATATCTCC CATGTTTCAG GTCCTTGAAA AACATACCGC TCTCCAGGAA 
ACCCAAAAGA TGCAAGCCAA AATATTGGTT GTGCATTACA TTAAGACTAG CACCGCTTCA 
CAAGTTTATC CTAAATGGCA CTTGGATATT TATACACAGA TACATCTTTC CCCGTCTTTG 
GGACCTAATT TTAATTTCCT GTGCATTAAC AAGAAATTTA TTTTTGAACT TAAAGTTTAT 
GTTCTGCTGT TTTTGCTTGT GCTCTTTACT GGTGAATATA TTGGAAATTT GGACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( XM_010801964.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800266.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X3, mRNA.

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CloneID	OBa45852
Clone ID Related Accession (Same CDS sequence)	XM_010801964.2 , XM_010801964.3
Accession Version	XM_010801964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGTATGGCA TGCAGCTGTA CCAGAATTAC ATGCATCCGT ATCAAGGCAG AAGTGGCTAC 
AGTTCTCAGA GTATATCTCC CATGTTTCAG GTCCTTGAAA AACATACCGC TCTCCAGGAA 
ACCCAAAAGA TGCAAGCCAA AATATTGGTT GTGCATTACA TTAAGACTAG CACCGCTTCA 
CAAGTTTATC CTAAATGGCA CTTGGATATT TATACACAGA TACATCTTTC CCCGTCTTTG 
GGACCTAATT TTAATTTCCT GTGCATTAAC AAGAAATTTA TTTTTGAACT TAAAGTTTAT 
GTTCTGCTGT TTTTGCTTGT GCTCTTTACT GGTGAATATA TTGGAAATTT GGACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800266.1
CDS	140..1519
Translation	MTTSLQDGQSAAGRAAARDSPLAAQVCGAAQGRGDARDLAPAPWLHARALLPPPDATRGCAADRRKKKDLDVLEMPSIPNPFPELCCSPFTSVLSAGLFPKANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGYSSQSISPMFQVLEKHTALQETQKMQAKILVVHYIKTSTASQVYPKWHLDIYTQIHLSPSLGPNFNFLCINKKFIFELKVYVLLFLLVLFTGEYIGNLDV

Target ORF information:

RefSeq Version	XM_010801964.3
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801964.3

ORF Insert Sequence:

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGTATGGCA TGCAGCTGTA CCAGAATTAC ATGCATCCGT ATCAAGGCAG AAGTGGCTAC 
AGTTCTCAGA GTATATCTCC CATGTTTCAG GTCCTTGAAA AACATACCGC TCTCCAGGAA 
ACCCAAAAGA TGCAAGCCAA AATATTGGTT GTGCATTACA TTAAGACTAG CACCGCTTCA 
CAAGTTTATC CTAAATGGCA CTTGGATATT TATACACAGA TACATCTTTC CCCGTCTTTG 
GGACCTAATT TTAATTTCCT GTGCATTAAC AAGAAATTTA TTTTTGAACT TAAAGTTTAT 
GTTCTGCTGT TTTTGCTTGT GCTCTTTACT GGTGAATATA TTGGAAATTT GGACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRB14 ( XM_015475191.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330677.1 Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X1, mRNA.

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CloneID	OBa87909
Clone ID Related Accession (Same CDS sequence)	XM_015475191.1
Accession Version	XM_015475191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	growth factor receptor-bound protein 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGAGAAGTA TATCAGAGAA TTCCCTGGTA GCAATGGATT TCTCAGGCCA GAAAACCAGA 
GTTATAGAAA ATCCCACTGA AGCCCTTTCC GTTGCAGTTG AAGAAGGACT AGCTTGGAGG 
AAAAAAGGAT GTTTACGCCT GGGCAGTCAC GGTAGCCCCA CTGCATCCTC ACAGAGCTCT 
GCCACAAGCA TGGCTATCCA CCGGTCCCAA CCATGGTTTC ACCACAAAAT TTCTAGAGAG 
GAAGCTCAGC GATTGATTAT TCAGCAAGGT CTAGTGGATG GAGTTTTCTT GGTACGGGAT 
AGTCAGAGTA ACCCCAAAAC TTTTGTACTG TCAATGAGTC ATGGACAAAA AATAAAGCAC 
TTTCAAATTA TACCGGTGGA AGATGATGGT GAGATGTTCC ACACCCTAGA TGATGGACAT 
ACAAGATTTA CGGATCTAAT CCAGTTGGTG GAGTTCTATC AGCTCAATAA GGGTGTTCTT 
CCTTGCAAGC TGAAACATTA TTGTGCTAGG ATTGCACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330677.1
CDS	154..1695
Translation	MTTSLQDGQSAAGRAAARDSPLAAQVCGAAQGRGDARDLAPAPWLHARALLPPPDATRGCAADRRKKKDLDVLEMPSIPNPFPELCCSPFTSVLSAGLFPKANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHVGLERTIEDHELVIEVLSNWGMEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFLLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKRSISENSLVAMDFSGQKTRVIENPTEALSVAVEEGLAWRKKGCLRLGSHGSPTASSQSSATSMAIHRSQPWFHHKISREEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Target ORF information:

RefSeq Version	XM_015475191.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth factor receptor bound protein 14 (GRB14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475191.1

ORF Insert Sequence:

ATGACCACTT CCCTGCAAGA TGGGCAGAGC GCCGCGGGTC GGGCGGCTGC CCGGGACTCG 
CCGCTGGCCG CCCAGGTGTG CGGCGCTGCC CAGGGGAGGG GCGACGCCCG CGACCTGGCG 
CCGGCCCCCT GGCTGCACGC GCGGGCGCTC CTGCCCCCTC CGGACGCGAC CCGCGGCTGT 
GCGGCAGACA GGAGAAAAAA GAAAGATCTT GATGTTCTGG AAATGCCATC TATTCCAAAC 
CCTTTTCCTG AGCTATGCTG CTCTCCATTT ACATCTGTGT TGTCAGCAGG CCTGTTTCCC 
AAAGCAAATT CAAGGAAAAA ACAGGTAATT AAAGTATACA GTGAAGATGA AACCAGCAGA 
GCTTTAGAGG TACCCAGTGA CATAACTGCC CGAGATGTTT GCCAGCTGTT GATCCTGAAG 
AATCATTACA TTGATGACCA CAGCTGGACC CTTTTTGAGC ACCTGCCTCA TGTAGGTCTA 
GAAAGAACAA TAGAAGACCA CGAGCTGGTG ATTGAAGTGC TATCTAACTG GGGAATGGAA 
GAAGAAAATA AGCTATACTT TAGAAAAAAT TATGCCAAAT ATGAATTCTT TAAAAACCCA 
ATGTATTTTT TTCCAGAGCA TATGGTGTCT TTTGCAACTG AAACCAATGG TGAAATATCC 
CCCACACAGA TTTTGCAGAT GTTTCTAAGT TCAAGCACAT ATCCTGAAAT CCATGGCTTC 
TTACATGCAA AAGAACAGGG AAAGAAGTCC TGGAAAAAGA TTTATTTCCT TTTAAGAAGA 
TCCGGTTTAT ATTTTTCTAC TAAAGGGACA TCAAAGGAAC CACGGCATTT GCAGTTTTTC 
AGCGAATTTG GCAATAGTGA TATTTATGTG TCACTGGCAG GCAAAAAAAA ACACGGAGCA 
CCGACTAACT ATGGATTCTG CTTTAAGCCT AACAAAGCGG GAGGGCCCCG AGACCTGAAA 
ATGCTCTGTG CAGAAGAAGA GCAGAGTAGG ACGTGCTGGG TGACCGCGAT TAGATTGCTT 
AAGAGAAGTA TATCAGAGAA TTCCCTGGTA GCAATGGATT TCTCAGGCCA GAAAACCAGA 
GTTATAGAAA ATCCCACTGA AGCCCTTTCC GTTGCAGTTG AAGAAGGACT AGCTTGGAGG 
AAAAAAGGAT GTTTACGCCT GGGCAGTCAC GGTAGCCCCA CTGCATCCTC ACAGAGCTCT 
GCCACAAGCA TGGCTATCCA CCGGTCCCAA CCATGGTTTC ACCACAAAAT TTCTAGAGAG 
GAAGCTCAGC GATTGATTAT TCAGCAAGGT CTAGTGGATG GAGTTTTCTT GGTACGGGAT 
AGTCAGAGTA ACCCCAAAAC TTTTGTACTG TCAATGAGTC ATGGACAAAA AATAAAGCAC 
TTTCAAATTA TACCGGTGGA AGATGATGGT GAGATGTTCC ACACCCTAGA TGATGGACAT 
ACAAGATTTA CGGATCTAAT CCAGTTGGTG GAGTTCTATC AGCTCAATAA GGGTGTTCTT 
CCTTGCAAGC TGAAACATTA TTGTGCTAGG ATTGCACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Grb14 mRNA levels during follicular deviation in cattle are higher in granulosa cells of subordinate compared to dominant follicles.
Reproduction in domestic animals = Zuchthygiene48(3)396-401(2013 Jun)
Bohrer RC,Rosa PR,Ferreira R,Bordignon V,Oliveira JF,Gon?alves PB

Growth factor receptor-bound protein 14: a new modulator of photoreceptor-specific cyclic-nucleotide-gated channel.
EMBO reports11(11)861-7(2010 Nov)
Gupta VK,Rajala A,Daly RJ,Rajala RV

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Lipid-protein interactions of growth factor receptor-bound protein 14 in insulin receptor signaling.
Biochemistry44(47)15461-71(2005 Nov)
Rajala RV,Chan MD,Rajala A

Identification of a NPXY motif in growth factor receptor-bound protein 14 (Grb14) and its interaction with the phosphotyrosine-binding (PTB) domain of IRS-1.
Biochemistry44(22)7929-35(2005 Jun)
Rajala RV,Chan MD

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GRB14 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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