EIF4G1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010801318.1	XP_010799620.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_015472316.2	XP_015327802.2	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_010801318.2	XP_010799620.2	eukaryotic translation initiation factor 4 gamma 1 isoform X9
XM_024995325.1	XP_024851093.1	eukaryotic translation initiation factor 4 gamma 1 isoform X5
XM_024995329.1	XP_024851097.1	eukaryotic translation initiation factor 4 gamma 1 isoform X6
XM_024995330.1	XP_024851098.1	eukaryotic translation initiation factor 4 gamma 1 isoform X7
XM_010801319.2	XP_010799621.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_015462063.1	XP_015317549.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_015462066.1	XP_015317552.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_010801319.3	XP_010799621.2	eukaryotic translation initiation factor 4 gamma 1 isoform X4
XM_010801320.1	XP_010799622.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_024995308.1	XP_024851076.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_024995332.1	XP_024851100.1	eukaryotic translation initiation factor 4 gamma 1 isoform X8
XM_015462064.1	XP_015317550.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_015462065.1	XP_015317551.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_015472316.1	XP_015327802.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_024995312.1	XP_024851080.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219179	XM_010801318.2 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219171	XM_024995308.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219172	XM_024995312.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219173	XM_015472316.2 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219174	XM_010801319.3 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219175	XM_024995325.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219176	XM_024995329.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219177	XM_024995330.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa219178	XM_024995332.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa45811	XM_015472316.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa45811	XM_010801318.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa45811	XM_015462064.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa45811	XM_015462063.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa45812	XM_010801319.2 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa45812	XM_015462065.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa45813	XM_015462066.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa45813	XM_010801320.1 Latest version!	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

EIF4G1 ( XM_010801318.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799620.2 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa219179
Clone ID Related Accession (Same CDS sequence)	XM_010801318.2
Accession Version	XM_010801318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGGA AGACGATCCG AATTCGAGAC 
CCAAACCAAG GAGGGAAGGA TATCACGGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC 
ACACCCACAC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AGGCTAATGG GGAGACACCC 
CAGGTTGCTG TCGTCGTCCG GCCAGATGAC CGGTCGCAGG GAGCAATCAT TGGGGAGCGG 
CCAGGGCTGC CTGGCCCAGA GCACAGCCCT TCAGAATCCC AGCCTTCATC ACCTTCTCCA 
ACCCCATCAC CACCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT 
GTTCCTGGGG ACACAATGAC AACAGGGATG ATACAGACGT CTGTAGAAGA ATCAACCCCC 
ACGCCCCCTG AAAGTGGGGA GCCGTGTTGC CTCTCTCCAG AACCCACTCC CCTTGCTGAA 
CCCATATTGG AAGTAGAAGT GACGCTGAGC AAACCAGTTC CAGAATCTGA GTTCTCTTCC 
AGTCCTCTCC AGGTTCCCAG CCCCCTGGCA TCTCACAAGG TGGAAATTCT TCCTGAGCCT 
AATGGCACAG TCCCATCTGA GAATTTGGAA CCAGAGGTGG AGTCAAGCCC AGAGCTTGCC 
CCTCTCCCTC CTCCAGCTTG TCCCTCTGAA TCCCCCATGC CTATTGCTCC AACTGCCCAA 
CCTGAGGAAC TACTCAACGG AGCCCCTTCA CCACCAGCTG TGGACTTAAG CCCAGTCAGT 
GAACCAGAGG AACAGGCCAA GGAGGCTATA GTGTCGGTGA CTCCCCCCAC TGTCCTTTCT 
GCTACCCCAG CTGTGGCTCC TCCAGTGGCT TCCCCTGCTC AGGAGGAGGA CATGGAGGAG 
GAGGAAGAAG AGGAAGAGGA AGGAGAAGCT GAGGGTGAGA AGGGAGGAGA GGAACCTCTC 
CCCCAAGAGA GCACCCCTGT CCCAGCCCAC CTATCCCAGA ATTCGGAGGT GGCAGTAGCC 
ACCCAAGTGG CAGTGTCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT CAATAAGAAG 
GAGGCTGTAG GAGACCTTCT AGATGCCTTC AAGGAGGCGA ACCCAGGGGT ACCAGAGGTG 
GAAAATCAGC CTCCTGCAGG CTGCAATCCC AGCCCAGAGC CTGAGGGCAG CAGTGTGCCC 
CTGCGGTCTG AGGAAGGAGA GGAGACCTGG GACTCAAAGG AAGACAAGAT TCAAAATGCA 
GAGAATATCC AGCCAGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA 
AACCTTGAGG AGAAAAAGCG TTATGACCGA GAGTTCCTGC TTGGCTTTCA GTTCATCTTT 
GCCAGTATGC AGAAGCCTGA GGGATTGCCC CACATCAGTG ATGTGGTGTT GGATAAGGCC 
AATAAAACAC CATTGCGACC GCTGGACCCC TCTAGACTTC AGGGCATAAA TTGTGGCCCA 
GACTTCACTC CGTCTTTTGC CAACCTTGGC CGACCAGCCC TTAGCAACCG TGGGCCCCCG 
AGGGGTGGGC CAGGTGGGGA GCTGCCCCGA GGGCCGCAGG CTGGCCTGGG ACCCCGGCGA 
TCTCAGCAGG GCCCCCGAAA GGAACCACGC AAGATCATTG CCACAGTGTC AATGACCGAA 
GATATAAAGC TGAACAAAGC AGAAAAGGCC TGGAAACCCA GTAGCAAGCG GACGGCGGCT 
GATAAGGACC GAGGAGAAGA GGATGCTGAT GGCAGCAAAA CCCAGGACCT GTTCCGCAGG 
GTGCGCTCCA TCTTGAATAA GCTGACACCC CAGATGTTCC AGCAGCTGAT GAAGCAGGTG 
ACGCAGCTAG CCATTGATAC CGAGGAACGT CTCAAAGGGG TCATTGACCT CATCTTCGAG 
AAGGCCATTT CAGAACCCAA CTTCTCCGTG GCCTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTGACTG TGAACTTCCG AAAACTGTTG 
TTAAATCGAT GTCAGAAGGA GTTTGAAAAA GACAAAGATG ATGATGAGGT TTTTGAGAAG 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAGGAAC GGGGACGCCT GAAAGAAGAG 
CTGGAAGAGG CTCGAGACAT AGCCCGGCGG CGCTCGTTAG GGAATATCAA GTTTATCGGG 
GAGTTGTTCA AATTGAAGAT GTTAACAGAG GCAATCATGC ATGACTGTGT GGTTAAACTA 
CTTAAGAACC ATGATGAAGA GTCCCTTGAA TGCCTTTGCC GTCTGCTCAC CACCATTGGC 
AAAGACCTGG ACTTTGAGAA GGCCAAGCCC CGGATGGATC AGTATTTCAA CCAGATGGAA 
AAAATCATTA AGGAAAAGAA GACTTCATCC CGGATCCGCT TTATGCTGCA GGATGTGCTG 
GATCTGCGAA AGAGCAACTG GGTGCCACGC CGTGGGGACC AGGGGCCCAA GACAATTGAC 
CAAATCCACA AGGAAGCTGA GATGGAGGAG CATCGGGAGC ACATAAAAGT GCAGCAGCTA 
ATGGCCAAGG GCAGCGACAA GCGTCGGGGT GGCCCTCCAG GCCCACCCAT CAGCCGTGGC 
CTTCCACTTG TGGATGATGG TGGCTGGAAC ACAGTACCCA TCAGCAAGGG CAGCCGCCCT 
ATTGACACTT CTCGACTCAC TAAGATCACA AAGCCTGGCT CCATTGATTC TAATAACCAG 
CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC 
AAGCCCTCCG ATGCAGCATC AGAAGCTGCT CGTCCAGCTA CTAGTACCTT GAATCGCTTC 
TCAGCCCTTC AACAAGCAGT ACCAACAGAA AGCACAGACA ACAGACGAGT GGTGCAGAGG 
AGTAGCTTGA GCCGGGAACG AGGTGAGAAA GCTGGGGACC GGGGAGACCG CCTAGAGCGG 
AGTGAACGGG GAGGTGACCG TGGTGACCGG CTTGATCGCG CCCGGACACC CGCCACCAAG 
CGGAGCTTCA GCAAGGAAGT GGAGGAACGG AGTAGAGAGC GGCCCTCTCA GCCTGATGGA 
CTACGCAAGG CAGCTAGCCT CACGGAGGAT CGGGACCGCG GGCGGGAAGC TGTGAAACGA 
GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG 
AAGAAATCCA GGGCCATCAT TGAGGAGTAC CTCCATCTCA ATGACATGAA GGAGGCAGTG 
CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC CTGCTCTTTA TCTTTGTACG GCACGGCATC 
GAGTCCACGC TGGAGCGCAG CGCCATCACG CGGGAGCACA TGGGCCGGCT GCTGCACCAG 
CTGCTCTGTG CTGGGCACCT CTCCACTGCT CAGTACTACC AAGGGCTATA TGAAATTCTA 
GAGTTGGCTG AAGACATGGA AATTGACATC CCTCATGTGT GGCTCTACCT AGCAGAATTG 
ATAACGCCCA TTCTGCAGGA AGGTGGGGTG CCAATGGGGG AGCTGTTCAG GGAAATAACA 
AAACCTCTGA GACCCCTGGG CAAGGCTGCT TCCCTGTTGC TGGAGATCCT GGGGCTCCTG 
TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG 
AAGGAATTTC TGCCTGAAGG CCAGGATGTC AGTGCCTTTG TCACTGCGCA GAAGGTGGAG 
TATACCCTGG GAGAGGAGTT AGAAGCCCCA GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG 
CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG GGCAGCAGTA ACCAGCGGGT GTTTGACTGG 
ATAGAGGCCA ACCTGAACGA GCAGCAGGTG ACATCCAACA CATTAGTTCG AGCCCTTATG 
ACAACTGTCT GCTATTCTGC AATTATCTTT GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG 
CTGAAAGCAC GAGCGAAACT GCTACAGAAA TACCTGTGTG ACGAGCAGAA GGAGCTGCAG 
GCGCTCTATG CCCTCCAGGC CCTTGTAGTG ACCTTAGACC AGCCCCCTAA CCTGCTTGGG 
ATGTTCTTCG ATGCGCTGTA TGACGAGGAC GTGGTGAAGG AGGACGCCTT CTATAGCTGG 
GAGAGTAGCA AGGACCCTGC TGAGCAGCAG GGCAAGGGCG TGGCCCTAAA ATCTGTCACG 
ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799620.2
CDS	471..4784
Translation	MNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_010801318.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801318.2

ORF Insert Sequence:

ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGGA AGACGATCCG AATTCGAGAC 
CCAAACCAAG GAGGGAAGGA TATCACGGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC 
ACACCCACAC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AGGCTAATGG GGAGACACCC 
CAGGTTGCTG TCGTCGTCCG GCCAGATGAC CGGTCGCAGG GAGCAATCAT TGGGGAGCGG 
CCAGGGCTGC CTGGCCCAGA GCACAGCCCT TCAGAATCCC AGCCTTCATC ACCTTCTCCA 
ACCCCATCAC CACCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT 
GTTCCTGGGG ACACAATGAC AACAGGGATG ATACAGACGT CTGTAGAAGA ATCAACCCCC 
ACGCCCCCTG AAAGTGGGGA GCCGTGTTGC CTCTCTCCAG AACCCACTCC CCTTGCTGAA 
CCCATATTGG AAGTAGAAGT GACGCTGAGC AAACCAGTTC CAGAATCTGA GTTCTCTTCC 
AGTCCTCTCC AGGTTCCCAG CCCCCTGGCA TCTCACAAGG TGGAAATTCT TCCTGAGCCT 
AATGGCACAG TCCCATCTGA GAATTTGGAA CCAGAGGTGG AGTCAAGCCC AGAGCTTGCC 
CCTCTCCCTC CTCCAGCTTG TCCCTCTGAA TCCCCCATGC CTATTGCTCC AACTGCCCAA 
CCTGAGGAAC TACTCAACGG AGCCCCTTCA CCACCAGCTG TGGACTTAAG CCCAGTCAGT 
GAACCAGAGG AACAGGCCAA GGAGGCTATA GTGTCGGTGA CTCCCCCCAC TGTCCTTTCT 
GCTACCCCAG CTGTGGCTCC TCCAGTGGCT TCCCCTGCTC AGGAGGAGGA CATGGAGGAG 
GAGGAAGAAG AGGAAGAGGA AGGAGAAGCT GAGGGTGAGA AGGGAGGAGA GGAACCTCTC 
CCCCAAGAGA GCACCCCTGT CCCAGCCCAC CTATCCCAGA ATTCGGAGGT GGCAGTAGCC 
ACCCAAGTGG CAGTGTCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT CAATAAGAAG 
GAGGCTGTAG GAGACCTTCT AGATGCCTTC AAGGAGGCGA ACCCAGGGGT ACCAGAGGTG 
GAAAATCAGC CTCCTGCAGG CTGCAATCCC AGCCCAGAGC CTGAGGGCAG CAGTGTGCCC 
CTGCGGTCTG AGGAAGGAGA GGAGACCTGG GACTCAAAGG AAGACAAGAT TCAAAATGCA 
GAGAATATCC AGCCAGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA 
AACCTTGAGG AGAAAAAGCG TTATGACCGA GAGTTCCTGC TTGGCTTTCA GTTCATCTTT 
GCCAGTATGC AGAAGCCTGA GGGATTGCCC CACATCAGTG ATGTGGTGTT GGATAAGGCC 
AATAAAACAC CATTGCGACC GCTGGACCCC TCTAGACTTC AGGGCATAAA TTGTGGCCCA 
GACTTCACTC CGTCTTTTGC CAACCTTGGC CGACCAGCCC TTAGCAACCG TGGGCCCCCG 
AGGGGTGGGC CAGGTGGGGA GCTGCCCCGA GGGCCGCAGG CTGGCCTGGG ACCCCGGCGA 
TCTCAGCAGG GCCCCCGAAA GGAACCACGC AAGATCATTG CCACAGTGTC AATGACCGAA 
GATATAAAGC TGAACAAAGC AGAAAAGGCC TGGAAACCCA GTAGCAAGCG GACGGCGGCT 
GATAAGGACC GAGGAGAAGA GGATGCTGAT GGCAGCAAAA CCCAGGACCT GTTCCGCAGG 
GTGCGCTCCA TCTTGAATAA GCTGACACCC CAGATGTTCC AGCAGCTGAT GAAGCAGGTG 
ACGCAGCTAG CCATTGATAC CGAGGAACGT CTCAAAGGGG TCATTGACCT CATCTTCGAG 
AAGGCCATTT CAGAACCCAA CTTCTCCGTG GCCTATGCCA ACATGTGCCG CTGCCTCATG 
GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTGACTG TGAACTTCCG AAAACTGTTG 
TTAAATCGAT GTCAGAAGGA GTTTGAAAAA GACAAAGATG ATGATGAGGT TTTTGAGAAG 
AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAGGAAC GGGGACGCCT GAAAGAAGAG 
CTGGAAGAGG CTCGAGACAT AGCCCGGCGG CGCTCGTTAG GGAATATCAA GTTTATCGGG 
GAGTTGTTCA AATTGAAGAT GTTAACAGAG GCAATCATGC ATGACTGTGT GGTTAAACTA 
CTTAAGAACC ATGATGAAGA GTCCCTTGAA TGCCTTTGCC GTCTGCTCAC CACCATTGGC 
AAAGACCTGG ACTTTGAGAA GGCCAAGCCC CGGATGGATC AGTATTTCAA CCAGATGGAA 
AAAATCATTA AGGAAAAGAA GACTTCATCC CGGATCCGCT TTATGCTGCA GGATGTGCTG 
GATCTGCGAA AGAGCAACTG GGTGCCACGC CGTGGGGACC AGGGGCCCAA GACAATTGAC 
CAAATCCACA AGGAAGCTGA GATGGAGGAG CATCGGGAGC ACATAAAAGT GCAGCAGCTA 
ATGGCCAAGG GCAGCGACAA GCGTCGGGGT GGCCCTCCAG GCCCACCCAT CAGCCGTGGC 
CTTCCACTTG TGGATGATGG TGGCTGGAAC ACAGTACCCA TCAGCAAGGG CAGCCGCCCT 
ATTGACACTT CTCGACTCAC TAAGATCACA AAGCCTGGCT CCATTGATTC TAATAACCAG 
CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC 
AAGCCCTCCG ATGCAGCATC AGAAGCTGCT CGTCCAGCTA CTAGTACCTT GAATCGCTTC 
TCAGCCCTTC AACAAGCAGT ACCAACAGAA AGCACAGACA ACAGACGAGT GGTGCAGAGG 
AGTAGCTTGA GCCGGGAACG AGGTGAGAAA GCTGGGGACC GGGGAGACCG CCTAGAGCGG 
AGTGAACGGG GAGGTGACCG TGGTGACCGG CTTGATCGCG CCCGGACACC CGCCACCAAG 
CGGAGCTTCA GCAAGGAAGT GGAGGAACGG AGTAGAGAGC GGCCCTCTCA GCCTGATGGA 
CTACGCAAGG CAGCTAGCCT CACGGAGGAT CGGGACCGCG GGCGGGAAGC TGTGAAACGA 
GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG 
AAGAAATCCA GGGCCATCAT TGAGGAGTAC CTCCATCTCA ATGACATGAA GGAGGCAGTG 
CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC CTGCTCTTTA TCTTTGTACG GCACGGCATC 
GAGTCCACGC TGGAGCGCAG CGCCATCACG CGGGAGCACA TGGGCCGGCT GCTGCACCAG 
CTGCTCTGTG CTGGGCACCT CTCCACTGCT CAGTACTACC AAGGGCTATA TGAAATTCTA 
GAGTTGGCTG AAGACATGGA AATTGACATC CCTCATGTGT GGCTCTACCT AGCAGAATTG 
ATAACGCCCA TTCTGCAGGA AGGTGGGGTG CCAATGGGGG AGCTGTTCAG GGAAATAACA 
AAACCTCTGA GACCCCTGGG CAAGGCTGCT TCCCTGTTGC TGGAGATCCT GGGGCTCCTG 
TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG 
AAGGAATTTC TGCCTGAAGG CCAGGATGTC AGTGCCTTTG TCACTGCGCA GAAGGTGGAG 
TATACCCTGG GAGAGGAGTT AGAAGCCCCA GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG 
CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG GGCAGCAGTA ACCAGCGGGT GTTTGACTGG 
ATAGAGGCCA ACCTGAACGA GCAGCAGGTG ACATCCAACA CATTAGTTCG AGCCCTTATG 
ACAACTGTCT GCTATTCTGC AATTATCTTT GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG 
CTGAAAGCAC GAGCGAAACT GCTACAGAAA TACCTGTGTG ACGAGCAGAA GGAGCTGCAG 
GCGCTCTATG CCCTCCAGGC CCTTGTAGTG ACCTTAGACC AGCCCCCTAA CCTGCTTGGG 
ATGTTCTTCG ATGCGCTGTA TGACGAGGAC GTGGTGAAGG AGGACGCCTT CTATAGCTGG 
GAGAGTAGCA AGGACCCTGC TGAGCAGCAG GGCAAGGGCG TGGCCCTAAA ATCTGTCACG 
ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_024995308.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851076.1 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219171
Clone ID Related Accession (Same CDS sequence)	XM_024995308.1
Accession Version	XM_024995308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGC AGGCTGGCCT GGGACCCCGG CGATCTCAGC AGGGCCCCCG AAAGGAACCA 
CGCAAGATCA TTGCCACAGT GTCAATGACC GAAGATATAA AGCTGAACAA AGCAGAAAAG 
GCCTGGAAAC CCAGTAGCAA GCGGACGGCG GCTGATAAGG ACCGAGGAGA AGAGGATGCT 
GATGGCAGCA AAACCCAGGA CCTGTTCCGC AGGGTGCGCT CCATCTTGAA TAAGCTGACA 
CCCCAGATGT TCCAGCAGCT GATGAAGCAG GTGACGCAGC TAGCCATTGA TACCGAGGAA 
CGTCTCAAAG GGGTCATTGA CCTCATCTTC GAGAAGGCCA TTTCAGAACC CAACTTCTCC 
GTGGCCTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG 
CCAACAGTGA CTGTGAACTT CCGAAAACTG TTGTTAAATC GATGTCAGAA GGAGTTTGAA 
AAAGACAAAG ATGATGATGA GGTTTTTGAG AAGAAGCAAA AAGAGATGGA TGAAGCTGCT 
ACGGCAGAGG AACGGGGACG CCTGAAAGAA GAGCTGGAAG AGGCTCGAGA CATAGCCCGG 
CGGCGCTCGT TAGGGAATAT CAAGTTTATC GGGGAGTTGT TCAAATTGAA GATGTTAACA 
GAGGCAATCA TGCATGACTG TGTGGTTAAA CTACTTAAGA ACCATGATGA AGAGTCCCTT 
GAATGCCTTT GCCGTCTGCT CACCACCATT GGCAAAGACC TGGACTTTGA GAAGGCCAAG 
CCCCGGATGG ATCAGTATTT CAACCAGATG GAAAAAATCA TTAAGGAAAA GAAGACTTCA 
TCCCGGATCC GCTTTATGCT GCAGGATGTG CTGGATCTGC GAAAGAGCAA CTGGGTGCCA 
CGCCGTGGGG ACCAGGGGCC CAAGACAATT GACCAAATCC ACAAGGAAGC TGAGATGGAG 
GAGCATCGGG AGCACATAAA AGTGCAGCAG CTAATGGCCA AGGGCAGCGA CAAGCGTCGG 
GGTGGCCCTC CAGGCCCACC CATCAGCCGT GGCCTTCCAC TTGTGGATGA TGGTGGCTGG 
AACACAGTAC CCATCAGCAA GGGCAGCCGC CCTATTGACA CTTCTCGACT CACTAAGATC 
ACAAAGCCTG GCTCCATTGA TTCTAATAAC CAGCTGTTTG CACCTGGAGG CCGATTGAGC 
TGGGGCAAGG GTAGCAGTGG AGGCTCAGGA GCCAAGCCCT CCGATGCAGC ATCAGAAGCT 
GCTCGTCCAG CTACTAGTAC CTTGAATCGC TTCTCAGCCC TTCAACAAGC AGTACCAACA 
GAAAGCACAG ACAACAGACG AGTGGTGCAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG 
AAAGCTGGGG ACCGGGGAGA CCGCCTAGAG CGGAGTGAAC GGGGAGGTGA CCGTGGTGAC 
CGGCTTGATC GCGCCCGGAC ACCCGCCACC AAGCGGAGCT TCAGCAAGGA AGTGGAGGAA 
CGGAGTAGAG AGCGGCCCTC TCAGCCTGAT GGACTACGCA AGGCAGCTAG CCTCACGGAG 
GATCGGGACC GCGGGCGGGA AGCTGTGAAA CGAGAAGCTG CCCTCCCCCC GGTGAGTCCC 
CCGAAGGCTG CACTCTCTGA AGAGGAGCTG GAGAAGAAAT CCAGGGCCAT CATTGAGGAG 
TACCTCCATC TCAATGACAT GAAGGAGGCA GTGCAGTGCG TGCAGGAGCT GGCTTCGCCC 
TCCCTGCTCT TTATCTTTGT ACGGCACGGC ATCGAGTCCA CGCTGGAGCG CAGCGCCATC 
ACGCGGGAGC ACATGGGCCG GCTGCTGCAC CAGCTGCTCT GTGCTGGGCA CCTCTCCACT 
GCTCAGTACT ACCAAGGGCT ATATGAAATT CTAGAGTTGG CTGAAGACAT GGAAATTGAC 
ATCCCTCATG TGTGGCTCTA CCTAGCAGAA TTGATAACGC CCATTCTGCA GGAAGGTGGG 
GTGCCAATGG GGGAGCTGTT CAGGGAAATA ACAAAACCTC TGAGACCCCT GGGCAAGGCT 
GCTTCCCTGT TGCTGGAGAT CCTGGGGCTC CTGTGCAAAA GCATGGGTCC TAAAAAGGTG 
GGGACGCTGT GGCGAGAGGC TGGACTCAGC TGGAAGGAAT TTCTGCCTGA AGGCCAGGAT 
GTCAGTGCCT TTGTCACTGC GCAGAAGGTG GAGTATACCC TGGGAGAGGA GTTAGAAGCC 
CCAGGCCAGA GGTTGCTGTC CTTTGAGGAG CTGCGCAGGC AGCTGGAGAA GCTGCTGCAG 
GAGGGCAGCA GTAACCAGCG GGTGTTTGAC TGGATAGAGG CCAACCTGAA CGAGCAGCAG 
GTGACATCCA ACACATTAGT TCGAGCCCTT ATGACAACTG TCTGCTATTC TGCAATTATC 
TTTGAGTCTT CTCTCCGAGT GGATGTTGCA GTGCTGAAAG CACGAGCGAA ACTGCTACAG 
AAATACCTGT GTGACGAGCA GAAGGAGCTG CAGGCGCTCT ATGCCCTCCA GGCCCTTGTA 
GTGACCTTAG ACCAGCCCCC TAACCTGCTT GGGATGTTCT TCGATGCGCT GTATGACGAG 
GACGTGGTGA AGGAGGACGC CTTCTATAGC TGGGAGAGTA GCAAGGACCC TGCTGAGCAG 
CAGGGCAAGG GCGTGGCCCT AAAATCTGTC ACGACTTTTT TCAAGTGGCT TCGTGAGGCG 
GAGGAGGAGG AGTCTGACCA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851076.1
CDS	248..5074
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_024995308.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995308.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGC AGGCTGGCCT GGGACCCCGG CGATCTCAGC AGGGCCCCCG AAAGGAACCA 
CGCAAGATCA TTGCCACAGT GTCAATGACC GAAGATATAA AGCTGAACAA AGCAGAAAAG 
GCCTGGAAAC CCAGTAGCAA GCGGACGGCG GCTGATAAGG ACCGAGGAGA AGAGGATGCT 
GATGGCAGCA AAACCCAGGA CCTGTTCCGC AGGGTGCGCT CCATCTTGAA TAAGCTGACA 
CCCCAGATGT TCCAGCAGCT GATGAAGCAG GTGACGCAGC TAGCCATTGA TACCGAGGAA 
CGTCTCAAAG GGGTCATTGA CCTCATCTTC GAGAAGGCCA TTTCAGAACC CAACTTCTCC 
GTGGCCTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG 
CCAACAGTGA CTGTGAACTT CCGAAAACTG TTGTTAAATC GATGTCAGAA GGAGTTTGAA 
AAAGACAAAG ATGATGATGA GGTTTTTGAG AAGAAGCAAA AAGAGATGGA TGAAGCTGCT 
ACGGCAGAGG AACGGGGACG CCTGAAAGAA GAGCTGGAAG AGGCTCGAGA CATAGCCCGG 
CGGCGCTCGT TAGGGAATAT CAAGTTTATC GGGGAGTTGT TCAAATTGAA GATGTTAACA 
GAGGCAATCA TGCATGACTG TGTGGTTAAA CTACTTAAGA ACCATGATGA AGAGTCCCTT 
GAATGCCTTT GCCGTCTGCT CACCACCATT GGCAAAGACC TGGACTTTGA GAAGGCCAAG 
CCCCGGATGG ATCAGTATTT CAACCAGATG GAAAAAATCA TTAAGGAAAA GAAGACTTCA 
TCCCGGATCC GCTTTATGCT GCAGGATGTG CTGGATCTGC GAAAGAGCAA CTGGGTGCCA 
CGCCGTGGGG ACCAGGGGCC CAAGACAATT GACCAAATCC ACAAGGAAGC TGAGATGGAG 
GAGCATCGGG AGCACATAAA AGTGCAGCAG CTAATGGCCA AGGGCAGCGA CAAGCGTCGG 
GGTGGCCCTC CAGGCCCACC CATCAGCCGT GGCCTTCCAC TTGTGGATGA TGGTGGCTGG 
AACACAGTAC CCATCAGCAA GGGCAGCCGC CCTATTGACA CTTCTCGACT CACTAAGATC 
ACAAAGCCTG GCTCCATTGA TTCTAATAAC CAGCTGTTTG CACCTGGAGG CCGATTGAGC 
TGGGGCAAGG GTAGCAGTGG AGGCTCAGGA GCCAAGCCCT CCGATGCAGC ATCAGAAGCT 
GCTCGTCCAG CTACTAGTAC CTTGAATCGC TTCTCAGCCC TTCAACAAGC AGTACCAACA 
GAAAGCACAG ACAACAGACG AGTGGTGCAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG 
AAAGCTGGGG ACCGGGGAGA CCGCCTAGAG CGGAGTGAAC GGGGAGGTGA CCGTGGTGAC 
CGGCTTGATC GCGCCCGGAC ACCCGCCACC AAGCGGAGCT TCAGCAAGGA AGTGGAGGAA 
CGGAGTAGAG AGCGGCCCTC TCAGCCTGAT GGACTACGCA AGGCAGCTAG CCTCACGGAG 
GATCGGGACC GCGGGCGGGA AGCTGTGAAA CGAGAAGCTG CCCTCCCCCC GGTGAGTCCC 
CCGAAGGCTG CACTCTCTGA AGAGGAGCTG GAGAAGAAAT CCAGGGCCAT CATTGAGGAG 
TACCTCCATC TCAATGACAT GAAGGAGGCA GTGCAGTGCG TGCAGGAGCT GGCTTCGCCC 
TCCCTGCTCT TTATCTTTGT ACGGCACGGC ATCGAGTCCA CGCTGGAGCG CAGCGCCATC 
ACGCGGGAGC ACATGGGCCG GCTGCTGCAC CAGCTGCTCT GTGCTGGGCA CCTCTCCACT 
GCTCAGTACT ACCAAGGGCT ATATGAAATT CTAGAGTTGG CTGAAGACAT GGAAATTGAC 
ATCCCTCATG TGTGGCTCTA CCTAGCAGAA TTGATAACGC CCATTCTGCA GGAAGGTGGG 
GTGCCAATGG GGGAGCTGTT CAGGGAAATA ACAAAACCTC TGAGACCCCT GGGCAAGGCT 
GCTTCCCTGT TGCTGGAGAT CCTGGGGCTC CTGTGCAAAA GCATGGGTCC TAAAAAGGTG 
GGGACGCTGT GGCGAGAGGC TGGACTCAGC TGGAAGGAAT TTCTGCCTGA AGGCCAGGAT 
GTCAGTGCCT TTGTCACTGC GCAGAAGGTG GAGTATACCC TGGGAGAGGA GTTAGAAGCC 
CCAGGCCAGA GGTTGCTGTC CTTTGAGGAG CTGCGCAGGC AGCTGGAGAA GCTGCTGCAG 
GAGGGCAGCA GTAACCAGCG GGTGTTTGAC TGGATAGAGG CCAACCTGAA CGAGCAGCAG 
GTGACATCCA ACACATTAGT TCGAGCCCTT ATGACAACTG TCTGCTATTC TGCAATTATC 
TTTGAGTCTT CTCTCCGAGT GGATGTTGCA GTGCTGAAAG CACGAGCGAA ACTGCTACAG 
AAATACCTGT GTGACGAGCA GAAGGAGCTG CAGGCGCTCT ATGCCCTCCA GGCCCTTGTA 
GTGACCTTAG ACCAGCCCCC TAACCTGCTT GGGATGTTCT TCGATGCGCT GTATGACGAG 
GACGTGGTGA AGGAGGACGC CTTCTATAGC TGGGAGAGTA GCAAGGACCC TGCTGAGCAG 
CAGGGCAAGG GCGTGGCCCT AAAATCTGTC ACGACTTTTT TCAAGTGGCT TCGTGAGGCG 
GAGGAGGAGG AGTCTGACCA CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_024995312.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851080.1 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA.

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CloneID	OBa219172
Clone ID Related Accession (Same CDS sequence)	XM_024995312.1
Accession Version	XM_024995312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC 
AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC 
TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT 
GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT 
CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG 
CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG 
GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA 
TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC 
CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC 
CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC 
CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG 
CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT 
GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC 
ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA 
AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG 
GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT 
CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA 
AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA 
GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG 
CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG 
AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG 
AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC 
CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC 
CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG 
CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT 
CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC 
CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG 
CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC 
AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA 
GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG 
ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT 
GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC 
GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG 
GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG 
GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851080.1
CDS	248..5071
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_024995312.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995312.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCGT CCGGCCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC 
AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC 
TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT 
GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT 
CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG 
CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG 
GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA 
TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC 
CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC 
CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC 
CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG 
CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT 
GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC 
ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA 
AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG 
GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT 
CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA 
AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA 
GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG 
CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG 
AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG 
AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC 
CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC 
CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG 
CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT 
CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC 
CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG 
CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC 
AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA 
GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG 
ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT 
GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC 
GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG 
GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG 
GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_015472316.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327802.2 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA.

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CloneID	OBa219173
Clone ID Related Accession (Same CDS sequence)	XM_015472316.2
Accession Version	XM_015472316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT 
CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC 
TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC 
CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT 
GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC 
ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA 
TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA 
GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT 
CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT 
CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC 
CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT 
CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA 
AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC 
ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG 
GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA 
GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG 
GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC 
AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG 
ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG 
TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA 
AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC 
CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG 
GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG 
TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG 
CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC 
CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC 
CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC 
CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC 
AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT 
GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG 
CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC 
AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA 
GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC 
ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG 
GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT 
TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA 
GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC 
TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA 
GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA 
CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT 
CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA 
GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA 
GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG 
AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA 
CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA 
TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC 
CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC 
AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT 
GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC 
TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG 
GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT 
CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG 
GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT 
AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC 
TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA 
AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327802.2
CDS	248..5053
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_015472316.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472316.2

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT 
CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC 
TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC 
CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT 
GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC 
ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA 
TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA 
GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT 
CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT 
CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC 
CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT 
CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA 
AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC 
ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG 
GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA 
GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG 
GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC 
AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG 
ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG 
TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA 
AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC 
CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG 
GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG 
TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG 
CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC 
CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC 
CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC 
CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC 
AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT 
GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG 
CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC 
AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA 
GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC 
ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG 
GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT 
TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA 
GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC 
TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA 
GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA 
CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT 
CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA 
GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA 
GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG 
AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA 
CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA 
TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC 
CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC 
AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT 
GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC 
TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG 
GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT 
CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG 
GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT 
AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC 
TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA 
AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_010801319.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799621.2 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA.

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CloneID	OBa219174
Clone ID Related Accession (Same CDS sequence)	XM_010801319.3
Accession Version	XM_010801319.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT 
CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC 
TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC 
CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT 
GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC 
ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA 
TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA 
GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT 
CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT 
CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC 
CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT 
CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA 
AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC 
ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG 
GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA 
GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG 
GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC 
AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG 
ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG 
TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA 
AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC 
CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGGC TGGCCTGGGA 
CCCCGGCGAT CTCAGCAGGG CCCCCGAAAG GAACCACGCA AGATCATTGC CACAGTGTCA 
ATGACCGAAG ATATAAAGCT GAACAAAGCA GAAAAGGCCT GGAAACCCAG TAGCAAGCGG 
ACGGCGGCTG ATAAGGACCG AGGAGAAGAG GATGCTGATG GCAGCAAAAC CCAGGACCTG 
TTCCGCAGGG TGCGCTCCAT CTTGAATAAG CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAGGTGA CGCAGCTAGC CATTGATACC GAGGAACGTC TCAAAGGGGT CATTGACCTC 
ATCTTCGAGA AGGCCATTTC AGAACCCAAC TTCTCCGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACA GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAACTGTTGT TAAATCGATG TCAGAAGGAG TTTGAAAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG GGGACGCCTG 
AAAGAAGAGC TGGAAGAGGC TCGAGACATA GCCCGGCGGC GCTCGTTAGG GAATATCAAG 
TTTATCGGGG AGTTGTTCAA ATTGAAGATG TTAACAGAGG CAATCATGCA TGACTGTGTG 
GTTAAACTAC TTAAGAACCA TGATGAAGAG TCCCTTGAAT GCCTTTGCCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAGAAG GCCAAGCCCC GGATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA GGAAAAGAAG ACTTCATCCC GGATCCGCTT TATGCTGCAG 
GATGTGCTGG ATCTGCGAAA GAGCAACTGG GTGCCACGCC GTGGGGACCA GGGGCCCAAG 
ACAATTGACC AAATCCACAA GGAAGCTGAG ATGGAGGAGC ATCGGGAGCA CATAAAAGTG 
CAGCAGCTAA TGGCCAAGGG CAGCGACAAG CGTCGGGGTG GCCCTCCAGG CCCACCCATC 
AGCCGTGGCC TTCCACTTGT GGATGATGGT GGCTGGAACA CAGTACCCAT CAGCAAGGGC 
AGCCGCCCTA TTGACACTTC TCGACTCACT AAGATCACAA AGCCTGGCTC CATTGATTCT 
AATAACCAGC TGTTTGCACC TGGAGGCCGA TTGAGCTGGG GCAAGGGTAG CAGTGGAGGC 
TCAGGAGCCA AGCCCTCCGA TGCAGCATCA GAAGCTGCTC GTCCAGCTAC TAGTACCTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCAGTA CCAACAGAAA GCACAGACAA CAGACGAGTG 
GTGCAGAGGA GTAGCTTGAG CCGGGAACGA GGTGAGAAAG CTGGGGACCG GGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGTGACCGT GGTGACCGGC TTGATCGCGC CCGGACACCC 
GCCACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAACGGA GTAGAGAGCG GCCCTCTCAG 
CCTGATGGAC TACGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGCGG GCGGGAAGCT 
GTGAAACGAG AAGCTGCCCT CCCCCCGGTG AGTCCCCCGA AGGCTGCACT CTCTGAAGAG 
GAGCTGGAGA AGAAATCCAG GGCCATCATT GAGGAGTACC TCCATCTCAA TGACATGAAG 
GAGGCAGTGC AGTGCGTGCA GGAGCTGGCT TCGCCCTCCC TGCTCTTTAT CTTTGTACGG 
CACGGCATCG AGTCCACGCT GGAGCGCAGC GCCATCACGC GGGAGCACAT GGGCCGGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCACCTC TCCACTGCTC AGTACTACCA AGGGCTATAT 
GAAATTCTAG AGTTGGCTGA AGACATGGAA ATTGACATCC CTCATGTGTG GCTCTACCTA 
GCAGAATTGA TAACGCCCAT TCTGCAGGAA GGTGGGGTGC CAATGGGGGA GCTGTTCAGG 
GAAATAACAA AACCTCTGAG ACCCCTGGGC AAGGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGGCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAGGCTGGA 
CTCAGCTGGA AGGAATTTCT GCCTGAAGGC CAGGATGTCA GTGCCTTTGT CACTGCGCAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTTA GAAGCCCCAG GCCAGAGGTT GCTGTCCTTT 
GAGGAGCTGC GCAGGCAGCT GGAGAAGCTG CTGCAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTTGACTGGA TAGAGGCCAA CCTGAACGAG CAGCAGGTGA CATCCAACAC ATTAGTTCGA 
GCCCTTATGA CAACTGTCTG CTATTCTGCA ATTATCTTTG AGTCTTCTCT CCGAGTGGAT 
GTTGCAGTGC TGAAAGCACG AGCGAAACTG CTACAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTGCAGG CGCTCTATGC CCTCCAGGCC CTTGTAGTGA CCTTAGACCA GCCCCCTAAC 
CTGCTTGGGA TGTTCTTCGA TGCGCTGTAT GACGAGGACG TGGTGAAGGA GGACGCCTTC 
TATAGCTGGG AGAGTAGCAA GGACCCTGCT GAGCAGCAGG GCAAGGGCGT GGCCCTAAAA 
TCTGTCACGA CTTTTTTCAA GTGGCTTCGT GAGGCGGAGG AGGAGGAGTC TGACCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799621.2
CDS	234..5036
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_010801319.3
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801319.3

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGCCGT TCCACATATG TTGTCCCGAC ACAGCAGTAT 
CCTGTGCAGC CGGGAGCCCC AAGCTTCTAC CCAGGTGCAA GCCCTACAGA GTTTGGGACC 
TACGCTGGCG CTTACTACCC AGCCCAGGGT GTGCAGCAGT TTCCCACTGG TGTAGCTCCC 
CCACCTGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG GAAGACGATC 
CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACGG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC ACCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAGGCTAAT 
GGGGAGACAC CCCAGGTTGC TGTCGTCGTC CGGCCAGATG ACCGGTCGCA GGGAGCAATC 
ATTGGGGAGC GGCCAGGGCT GCCTGGCCCA GAGCACAGCC CTTCAGAATC CCAGCCTTCA 
TCACCTTCTC CAACCCCATC ACCACCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTGTTCCTGG GGACACAATG ACAACAGGGA TGATACAGAC GTCTGTAGAA 
GAATCAACCC CCACGCCCCC TGAAAGTGGG GAGCCGTGTT GCCTCTCTCC AGAACCCACT 
CCCCTTGCTG AACCCATATT GGAAGTAGAA GTGACGCTGA GCAAACCAGT TCCAGAATCT 
GAGTTCTCTT CCAGTCCTCT CCAGGTTCCC AGCCCCCTGG CATCTCACAA GGTGGAAATT 
CTTCCTGAGC CTAATGGCAC AGTCCCATCT GAGAATTTGG AACCAGAGGT GGAGTCAAGC 
CCAGAGCTTG CCCCTCTCCC TCCTCCAGCT TGTCCCTCTG AATCCCCCAT GCCTATTGCT 
CCAACTGCCC AACCTGAGGA ACTACTCAAC GGAGCCCCTT CACCACCAGC TGTGGACTTA 
AGCCCAGTCA GTGAACCAGA GGAACAGGCC AAGGAGGCTA TAGTGTCGGT GACTCCCCCC 
ACTGTCCTTT CTGCTACCCC AGCTGTGGCT CCTCCAGTGG CTTCCCCTGC TCAGGAGGAG 
GACATGGAGG AGGAGGAAGA AGAGGAAGAG GAAGGAGAAG CTGAGGGTGA GAAGGGAGGA 
GAGGAACCTC TCCCCCAAGA GAGCACCCCT GTCCCAGCCC ACCTATCCCA GAATTCGGAG 
GTGGCAGTAG CCACCCAAGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTCAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGC GAACCCAGGG 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCTGCAATC CCAGCCCAGA GCCTGAGGGC 
AGCAGTGTGC CCCTGCGGTC TGAGGAAGGA GAGGAGACCT GGGACTCAAA GGAAGACAAG 
ATTCAAAATG CAGAGAATAT CCAGCCAGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTTGA GGAGAAAAAG CGTTATGACC GAGAGTTCCT GCTTGGCTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCT GAGGGATTGC CCCACATCAG TGATGTGGTG 
TTGGATAAGG CCAATAAAAC ACCATTGCGA CCGCTGGACC CCTCTAGACT TCAGGGCATA 
AATTGTGGCC CAGACTTCAC TCCGTCTTTT GCCAACCTTG GCCGACCAGC CCTTAGCAAC 
CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGGC TGGCCTGGGA 
CCCCGGCGAT CTCAGCAGGG CCCCCGAAAG GAACCACGCA AGATCATTGC CACAGTGTCA 
ATGACCGAAG ATATAAAGCT GAACAAAGCA GAAAAGGCCT GGAAACCCAG TAGCAAGCGG 
ACGGCGGCTG ATAAGGACCG AGGAGAAGAG GATGCTGATG GCAGCAAAAC CCAGGACCTG 
TTCCGCAGGG TGCGCTCCAT CTTGAATAAG CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAGGTGA CGCAGCTAGC CATTGATACC GAGGAACGTC TCAAAGGGGT CATTGACCTC 
ATCTTCGAGA AGGCCATTTC AGAACCCAAC TTCTCCGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACA GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAACTGTTGT TAAATCGATG TCAGAAGGAG TTTGAAAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG GGGACGCCTG 
AAAGAAGAGC TGGAAGAGGC TCGAGACATA GCCCGGCGGC GCTCGTTAGG GAATATCAAG 
TTTATCGGGG AGTTGTTCAA ATTGAAGATG TTAACAGAGG CAATCATGCA TGACTGTGTG 
GTTAAACTAC TTAAGAACCA TGATGAAGAG TCCCTTGAAT GCCTTTGCCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAGAAG GCCAAGCCCC GGATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA GGAAAAGAAG ACTTCATCCC GGATCCGCTT TATGCTGCAG 
GATGTGCTGG ATCTGCGAAA GAGCAACTGG GTGCCACGCC GTGGGGACCA GGGGCCCAAG 
ACAATTGACC AAATCCACAA GGAAGCTGAG ATGGAGGAGC ATCGGGAGCA CATAAAAGTG 
CAGCAGCTAA TGGCCAAGGG CAGCGACAAG CGTCGGGGTG GCCCTCCAGG CCCACCCATC 
AGCCGTGGCC TTCCACTTGT GGATGATGGT GGCTGGAACA CAGTACCCAT CAGCAAGGGC 
AGCCGCCCTA TTGACACTTC TCGACTCACT AAGATCACAA AGCCTGGCTC CATTGATTCT 
AATAACCAGC TGTTTGCACC TGGAGGCCGA TTGAGCTGGG GCAAGGGTAG CAGTGGAGGC 
TCAGGAGCCA AGCCCTCCGA TGCAGCATCA GAAGCTGCTC GTCCAGCTAC TAGTACCTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCAGTA CCAACAGAAA GCACAGACAA CAGACGAGTG 
GTGCAGAGGA GTAGCTTGAG CCGGGAACGA GGTGAGAAAG CTGGGGACCG GGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGTGACCGT GGTGACCGGC TTGATCGCGC CCGGACACCC 
GCCACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAACGGA GTAGAGAGCG GCCCTCTCAG 
CCTGATGGAC TACGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGCGG GCGGGAAGCT 
GTGAAACGAG AAGCTGCCCT CCCCCCGGTG AGTCCCCCGA AGGCTGCACT CTCTGAAGAG 
GAGCTGGAGA AGAAATCCAG GGCCATCATT GAGGAGTACC TCCATCTCAA TGACATGAAG 
GAGGCAGTGC AGTGCGTGCA GGAGCTGGCT TCGCCCTCCC TGCTCTTTAT CTTTGTACGG 
CACGGCATCG AGTCCACGCT GGAGCGCAGC GCCATCACGC GGGAGCACAT GGGCCGGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCACCTC TCCACTGCTC AGTACTACCA AGGGCTATAT 
GAAATTCTAG AGTTGGCTGA AGACATGGAA ATTGACATCC CTCATGTGTG GCTCTACCTA 
GCAGAATTGA TAACGCCCAT TCTGCAGGAA GGTGGGGTGC CAATGGGGGA GCTGTTCAGG 
GAAATAACAA AACCTCTGAG ACCCCTGGGC AAGGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGGCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAGGCTGGA 
CTCAGCTGGA AGGAATTTCT GCCTGAAGGC CAGGATGTCA GTGCCTTTGT CACTGCGCAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTTA GAAGCCCCAG GCCAGAGGTT GCTGTCCTTT 
GAGGAGCTGC GCAGGCAGCT GGAGAAGCTG CTGCAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTTGACTGGA TAGAGGCCAA CCTGAACGAG CAGCAGGTGA CATCCAACAC ATTAGTTCGA 
GCCCTTATGA CAACTGTCTG CTATTCTGCA ATTATCTTTG AGTCTTCTCT CCGAGTGGAT 
GTTGCAGTGC TGAAAGCACG AGCGAAACTG CTACAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTGCAGG CGCTCTATGC CCTCCAGGCC CTTGTAGTGA CCTTAGACCA GCCCCCTAAC 
CTGCTTGGGA TGTTCTTCGA TGCGCTGTAT GACGAGGACG TGGTGAAGGA GGACGCCTTC 
TATAGCTGGG AGAGTAGCAA GGACCCTGCT GAGCAGCAGG GCAAGGGCGT GGCCCTAAAA 
TCTGTCACGA CTTTTTTCAA GTGGCTTCGT GAGGCGGAGG AGGAGGAGTC TGACCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_024995325.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851093.1 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA.

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CloneID	OBa219175
Clone ID Related Accession (Same CDS sequence)	XM_024995325.1
Accession Version	XM_024995325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA 
GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG 
ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC 
CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC 
TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG 
GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC 
CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG 
AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC 
CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC 
GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC 
CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC 
CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA 
ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT 
GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA 
GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT 
CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA 
TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA 
GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC 
AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG 
GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG 
GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA 
GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC 
CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG 
TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC 
CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT 
GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA 
GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA 
GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA 
AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG 
CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG 
CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT 
TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT 
GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC CTGGGACCCC GGCGATCTCA GCAGGGCCCC 
CGAAAGGAAC CACGCAAGAT CATTGCCACA GTGTCAATGA CCGAAGATAT AAAGCTGAAC 
AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC AAGCGGACGG CGGCTGATAA GGACCGAGGA 
GAAGAGGATG CTGATGGCAG CAAAACCCAG GACCTGTTCC GCAGGGTGCG CTCCATCTTG 
AATAAGCTGA CACCCCAGAT GTTCCAGCAG CTGATGAAGC AGGTGACGCA GCTAGCCATT 
GATACCGAGG AACGTCTCAA AGGGGTCATT GACCTCATCT TCGAGAAGGC CATTTCAGAA 
CCCAACTTCT CCGTGGCCTA TGCCAACATG TGCCGCTGCC TCATGGCGCT GAAAGTGCCC 
ACTACAGAAA AGCCAACAGT GACTGTGAAC TTCCGAAAAC TGTTGTTAAA TCGATGTCAG 
AAGGAGTTTG AAAAAGACAA AGATGATGAT GAGGTTTTTG AGAAGAAGCA AAAAGAGATG 
GATGAAGCTG CTACGGCAGA GGAACGGGGA CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA 
GACATAGCCC GGCGGCGCTC GTTAGGGAAT ATCAAGTTTA TCGGGGAGTT GTTCAAATTG 
AAGATGTTAA CAGAGGCAAT CATGCATGAC TGTGTGGTTA AACTACTTAA GAACCATGAT 
GAAGAGTCCC TTGAATGCCT TTGCCGTCTG CTCACCACCA TTGGCAAAGA CCTGGACTTT 
GAGAAGGCCA AGCCCCGGAT GGATCAGTAT TTCAACCAGA TGGAAAAAAT CATTAAGGAA 
AAGAAGACTT CATCCCGGAT CCGCTTTATG CTGCAGGATG TGCTGGATCT GCGAAAGAGC 
AACTGGGTGC CACGCCGTGG GGACCAGGGG CCCAAGACAA TTGACCAAAT CCACAAGGAA 
GCTGAGATGG AGGAGCATCG GGAGCACATA AAAGTGCAGC AGCTAATGGC CAAGGGCAGC 
GACAAGCGTC GGGGTGGCCC TCCAGGCCCA CCCATCAGCC GTGGCCTTCC ACTTGTGGAT 
GATGGTGGCT GGAACACAGT ACCCATCAGC AAGGGCAGCC GCCCTATTGA CACTTCTCGA 
CTCACTAAGA TCACAAAGCC TGGCTCCATT GATTCTAATA ACCAGCTGTT TGCACCTGGA 
GGCCGATTGA GCTGGGGCAA GGGTAGCAGT GGAGGCTCAG GAGCCAAGCC CTCCGATGCA 
GCATCAGAAG CTGCTCGTCC AGCTACTAGT ACCTTGAATC GCTTCTCAGC CCTTCAACAA 
GCAGTACCAA CAGAAAGCAC AGACAACAGA CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG 
GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGCCTAG AGCGGAGTGA ACGGGGAGGT 
GACCGTGGTG ACCGGCTTGA TCGCGCCCGG ACACCCGCCA CCAAGCGGAG CTTCAGCAAG 
GAAGTGGAGG AACGGAGTAG AGAGCGGCCC TCTCAGCCTG ATGGACTACG CAAGGCAGCT 
AGCCTCACGG AGGATCGGGA CCGCGGGCGG GAAGCTGTGA AACGAGAAGC TGCCCTCCCC 
CCGGTGAGTC CCCCGAAGGC TGCACTCTCT GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC 
ATCATTGAGG AGTACCTCCA TCTCAATGAC ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG 
CTGGCTTCGC CCTCCCTGCT CTTTATCTTT GTACGGCACG GCATCGAGTC CACGCTGGAG 
CGCAGCGCCA TCACGCGGGA GCACATGGGC CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG 
CACCTCTCCA CTGCTCAGTA CTACCAAGGG CTATATGAAA TTCTAGAGTT GGCTGAAGAC 
ATGGAAATTG ACATCCCTCA TGTGTGGCTC TACCTAGCAG AATTGATAAC GCCCATTCTG 
CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG TTCAGGGAAA TAACAAAACC TCTGAGACCC 
CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG ATCCTGGGGC TCCTGTGCAA AAGCATGGGT 
CCTAAAAAGG TGGGGACGCT GTGGCGAGAG GCTGGACTCA GCTGGAAGGA ATTTCTGCCT 
GAAGGCCAGG ATGTCAGTGC CTTTGTCACT GCGCAGAAGG TGGAGTATAC CCTGGGAGAG 
GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG 
AAGCTGCTGC AGGAGGGCAG CAGTAACCAG CGGGTGTTTG ACTGGATAGA GGCCAACCTG 
AACGAGCAGC AGGTGACATC CAACACATTA GTTCGAGCCC TTATGACAAC TGTCTGCTAT 
TCTGCAATTA TCTTTGAGTC TTCTCTCCGA GTGGATGTTG CAGTGCTGAA AGCACGAGCG 
AAACTGCTAC AGAAATACCT GTGTGACGAG CAGAAGGAGC TGCAGGCGCT CTATGCCCTC 
CAGGCCCTTG TAGTGACCTT AGACCAGCCC CCTAACCTGC TTGGGATGTT CTTCGATGCG 
CTGTATGACG AGGACGTGGT GAAGGAGGAC GCCTTCTATA GCTGGGAGAG TAGCAAGGAC 
CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC CTAAAATCTG TCACGACTTT TTTCAAGTGG 
CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851093.1
CDS	248..4966
Translation	MNKAPQPTGPPPAPSPGLPQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_024995325.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995325.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA 
GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG 
ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC 
CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC 
TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG 
GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC 
CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG 
AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC 
CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC 
GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC 
CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC 
CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA 
ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT 
GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA 
GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT 
CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA 
TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA 
GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC 
AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG 
GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG 
GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA 
GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC 
CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG 
TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC 
CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT 
GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA 
GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA 
GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA 
AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG 
CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG 
CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT 
TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT 
GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC CTGGGACCCC GGCGATCTCA GCAGGGCCCC 
CGAAAGGAAC CACGCAAGAT CATTGCCACA GTGTCAATGA CCGAAGATAT AAAGCTGAAC 
AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC AAGCGGACGG CGGCTGATAA GGACCGAGGA 
GAAGAGGATG CTGATGGCAG CAAAACCCAG GACCTGTTCC GCAGGGTGCG CTCCATCTTG 
AATAAGCTGA CACCCCAGAT GTTCCAGCAG CTGATGAAGC AGGTGACGCA GCTAGCCATT 
GATACCGAGG AACGTCTCAA AGGGGTCATT GACCTCATCT TCGAGAAGGC CATTTCAGAA 
CCCAACTTCT CCGTGGCCTA TGCCAACATG TGCCGCTGCC TCATGGCGCT GAAAGTGCCC 
ACTACAGAAA AGCCAACAGT GACTGTGAAC TTCCGAAAAC TGTTGTTAAA TCGATGTCAG 
AAGGAGTTTG AAAAAGACAA AGATGATGAT GAGGTTTTTG AGAAGAAGCA AAAAGAGATG 
GATGAAGCTG CTACGGCAGA GGAACGGGGA CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA 
GACATAGCCC GGCGGCGCTC GTTAGGGAAT ATCAAGTTTA TCGGGGAGTT GTTCAAATTG 
AAGATGTTAA CAGAGGCAAT CATGCATGAC TGTGTGGTTA AACTACTTAA GAACCATGAT 
GAAGAGTCCC TTGAATGCCT TTGCCGTCTG CTCACCACCA TTGGCAAAGA CCTGGACTTT 
GAGAAGGCCA AGCCCCGGAT GGATCAGTAT TTCAACCAGA TGGAAAAAAT CATTAAGGAA 
AAGAAGACTT CATCCCGGAT CCGCTTTATG CTGCAGGATG TGCTGGATCT GCGAAAGAGC 
AACTGGGTGC CACGCCGTGG GGACCAGGGG CCCAAGACAA TTGACCAAAT CCACAAGGAA 
GCTGAGATGG AGGAGCATCG GGAGCACATA AAAGTGCAGC AGCTAATGGC CAAGGGCAGC 
GACAAGCGTC GGGGTGGCCC TCCAGGCCCA CCCATCAGCC GTGGCCTTCC ACTTGTGGAT 
GATGGTGGCT GGAACACAGT ACCCATCAGC AAGGGCAGCC GCCCTATTGA CACTTCTCGA 
CTCACTAAGA TCACAAAGCC TGGCTCCATT GATTCTAATA ACCAGCTGTT TGCACCTGGA 
GGCCGATTGA GCTGGGGCAA GGGTAGCAGT GGAGGCTCAG GAGCCAAGCC CTCCGATGCA 
GCATCAGAAG CTGCTCGTCC AGCTACTAGT ACCTTGAATC GCTTCTCAGC CCTTCAACAA 
GCAGTACCAA CAGAAAGCAC AGACAACAGA CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG 
GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGCCTAG AGCGGAGTGA ACGGGGAGGT 
GACCGTGGTG ACCGGCTTGA TCGCGCCCGG ACACCCGCCA CCAAGCGGAG CTTCAGCAAG 
GAAGTGGAGG AACGGAGTAG AGAGCGGCCC TCTCAGCCTG ATGGACTACG CAAGGCAGCT 
AGCCTCACGG AGGATCGGGA CCGCGGGCGG GAAGCTGTGA AACGAGAAGC TGCCCTCCCC 
CCGGTGAGTC CCCCGAAGGC TGCACTCTCT GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC 
ATCATTGAGG AGTACCTCCA TCTCAATGAC ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG 
CTGGCTTCGC CCTCCCTGCT CTTTATCTTT GTACGGCACG GCATCGAGTC CACGCTGGAG 
CGCAGCGCCA TCACGCGGGA GCACATGGGC CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG 
CACCTCTCCA CTGCTCAGTA CTACCAAGGG CTATATGAAA TTCTAGAGTT GGCTGAAGAC 
ATGGAAATTG ACATCCCTCA TGTGTGGCTC TACCTAGCAG AATTGATAAC GCCCATTCTG 
CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG TTCAGGGAAA TAACAAAACC TCTGAGACCC 
CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG ATCCTGGGGC TCCTGTGCAA AAGCATGGGT 
CCTAAAAAGG TGGGGACGCT GTGGCGAGAG GCTGGACTCA GCTGGAAGGA ATTTCTGCCT 
GAAGGCCAGG ATGTCAGTGC CTTTGTCACT GCGCAGAAGG TGGAGTATAC CCTGGGAGAG 
GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG 
AAGCTGCTGC AGGAGGGCAG CAGTAACCAG CGGGTGTTTG ACTGGATAGA GGCCAACCTG 
AACGAGCAGC AGGTGACATC CAACACATTA GTTCGAGCCC TTATGACAAC TGTCTGCTAT 
TCTGCAATTA TCTTTGAGTC TTCTCTCCGA GTGGATGTTG CAGTGCTGAA AGCACGAGCG 
AAACTGCTAC AGAAATACCT GTGTGACGAG CAGAAGGAGC TGCAGGCGCT CTATGCCCTC 
CAGGCCCTTG TAGTGACCTT AGACCAGCCC CCTAACCTGC TTGGGATGTT CTTCGATGCG 
CTGTATGACG AGGACGTGGT GAAGGAGGAC GCCTTCTATA GCTGGGAGAG TAGCAAGGAC 
CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC CTAAAATCTG TCACGACTTT TTTCAAGTGG 
CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_024995329.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851097.1 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa219176
Clone ID Related Accession (Same CDS sequence)	XM_024995329.1
Accession Version	XM_024995329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA 
GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG 
ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC 
CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC 
TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG 
GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC 
CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG 
AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC 
CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC 
GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC 
CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC 
CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA 
ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT 
GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA 
GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT 
CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA 
TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA 
GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC 
AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG 
GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG 
GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA 
GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC 
CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG 
TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC 
CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT 
GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA 
GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA 
GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA 
AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG 
CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG 
CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT 
TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT 
GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG GGACCCCGGC GATCTCAGCA GGGCCCCCGA 
AAGGAACCAC GCAAGATCAT TGCCACAGTG TCAATGACCG AAGATATAAA GCTGAACAAA 
GCAGAAAAGG CCTGGAAACC CAGTAGCAAG CGGACGGCGG CTGATAAGGA CCGAGGAGAA 
GAGGATGCTG ATGGCAGCAA AACCCAGGAC CTGTTCCGCA GGGTGCGCTC CATCTTGAAT 
AAGCTGACAC CCCAGATGTT CCAGCAGCTG ATGAAGCAGG TGACGCAGCT AGCCATTGAT 
ACCGAGGAAC GTCTCAAAGG GGTCATTGAC CTCATCTTCG AGAAGGCCAT TTCAGAACCC 
AACTTCTCCG TGGCCTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT 
ACAGAAAAGC CAACAGTGAC TGTGAACTTC CGAAAACTGT TGTTAAATCG ATGTCAGAAG 
GAGTTTGAAA AAGACAAAGA TGATGATGAG GTTTTTGAGA AGAAGCAAAA AGAGATGGAT 
GAAGCTGCTA CGGCAGAGGA ACGGGGACGC CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC 
ATAGCCCGGC GGCGCTCGTT AGGGAATATC AAGTTTATCG GGGAGTTGTT CAAATTGAAG 
ATGTTAACAG AGGCAATCAT GCATGACTGT GTGGTTAAAC TACTTAAGAA CCATGATGAA 
GAGTCCCTTG AATGCCTTTG CCGTCTGCTC ACCACCATTG GCAAAGACCT GGACTTTGAG 
AAGGCCAAGC CCCGGATGGA TCAGTATTTC AACCAGATGG AAAAAATCAT TAAGGAAAAG 
AAGACTTCAT CCCGGATCCG CTTTATGCTG CAGGATGTGC TGGATCTGCG AAAGAGCAAC 
TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC AAGACAATTG ACCAAATCCA CAAGGAAGCT 
GAGATGGAGG AGCATCGGGA GCACATAAAA GTGCAGCAGC TAATGGCCAA GGGCAGCGAC 
AAGCGTCGGG GTGGCCCTCC AGGCCCACCC ATCAGCCGTG GCCTTCCACT TGTGGATGAT 
GGTGGCTGGA ACACAGTACC CATCAGCAAG GGCAGCCGCC CTATTGACAC TTCTCGACTC 
ACTAAGATCA CAAAGCCTGG CTCCATTGAT TCTAATAACC AGCTGTTTGC ACCTGGAGGC 
CGATTGAGCT GGGGCAAGGG TAGCAGTGGA GGCTCAGGAG CCAAGCCCTC CGATGCAGCA 
TCAGAAGCTG CTCGTCCAGC TACTAGTACC TTGAATCGCT TCTCAGCCCT TCAACAAGCA 
GTACCAACAG AAAGCACAGA CAACAGACGA GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA 
CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC CGCCTAGAGC GGAGTGAACG GGGAGGTGAC 
CGTGGTGACC GGCTTGATCG CGCCCGGACA CCCGCCACCA AGCGGAGCTT CAGCAAGGAA 
GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT CAGCCTGATG GACTACGCAA GGCAGCTAGC 
CTCACGGAGG ATCGGGACCG CGGGCGGGAA GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG 
GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA GAGGAGCTGG AGAAGAAATC CAGGGCCATC 
ATTGAGGAGT ACCTCCATCT CAATGACATG AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG 
GCTTCGCCCT CCCTGCTCTT TATCTTTGTA CGGCACGGCA TCGAGTCCAC GCTGGAGCGC 
AGCGCCATCA CGCGGGAGCA CATGGGCCGG CTGCTGCACC AGCTGCTCTG TGCTGGGCAC 
CTCTCCACTG CTCAGTACTA CCAAGGGCTA TATGAAATTC TAGAGTTGGC TGAAGACATG 
GAAATTGACA TCCCTCATGT GTGGCTCTAC CTAGCAGAAT TGATAACGCC CATTCTGCAG 
GAAGGTGGGG TGCCAATGGG GGAGCTGTTC AGGGAAATAA CAAAACCTCT GAGACCCCTG 
GGCAAGGCTG CTTCCCTGTT GCTGGAGATC CTGGGGCTCC TGTGCAAAAG CATGGGTCCT 
AAAAAGGTGG GGACGCTGTG GCGAGAGGCT GGACTCAGCT GGAAGGAATT TCTGCCTGAA 
GGCCAGGATG TCAGTGCCTT TGTCACTGCG CAGAAGGTGG AGTATACCCT GGGAGAGGAG 
TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG 
CTGCTGCAGG AGGGCAGCAG TAACCAGCGG GTGTTTGACT GGATAGAGGC CAACCTGAAC 
GAGCAGCAGG TGACATCCAA CACATTAGTT CGAGCCCTTA TGACAACTGT CTGCTATTCT 
GCAATTATCT TTGAGTCTTC TCTCCGAGTG GATGTTGCAG TGCTGAAAGC ACGAGCGAAA 
CTGCTACAGA AATACCTGTG TGACGAGCAG AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG 
GCCCTTGTAG TGACCTTAGA CCAGCCCCCT AACCTGCTTG GGATGTTCTT CGATGCGCTG 
TATGACGAGG ACGTGGTGAA GGAGGACGCC TTCTATAGCT GGGAGAGTAG CAAGGACCCT 
GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA AAATCTGTCA CGACTTTTTT CAAGTGGCTT 
CGTGAGGCGG AGGAGGAGGA GTCTGACCAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851097.1
CDS	229..4944
Translation	MNKAPQPTGPPPAPSPGLPQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_024995329.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995329.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CACTTCTACC CTAGCCGGGC CCAGCCCCCG AGCAGTGCAG CCTCCCGAGT GCAGAGTGCA 
GCCCCTGCCC GCCCTGGCCC AGCTGCCCAT GTCTACCCTG CTGGATCCCA AGTAATGATG 
ATCCCTTCCC AGATCTCCTA CCCAGCCTCC CAGGGGGCCT ACTACATCCC TGGACAGGGC 
CGTTCCACAT ATGTTGTCCC GACACAGCAG TATCCTGTGC AGCCGGGAGC CCCAAGCTTC 
TACCCAGGTG CAAGCCCTAC AGAGTTTGGG ACCTACGCTG GCGCTTACTA CCCAGCCCAG 
GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT CCCCCACCTG TTTTGATGAA CCAGCCACCC 
CAGATTGCTC CCAAGAGGGA GCGGAAGACG ATCCGAATTC GAGACCCAAA CCAAGGAGGG 
AAGGATATCA CGGAGGAGAT CATGTCTGGG GCCCGCACTG CCTCCACACC CACACCTCCC 
CAGACGGGAG GCGGTCTGGA GCCTCAGGCT AATGGGGAGA CACCCCAGGT TGCTGTCGTC 
GTCCGGCCAG ATGACCGGTC GCAGGGAGCA ATCATTGGGG AGCGGCCAGG GCTGCCTGGC 
CCAGAGCACA GCCCTTCAGA ATCCCAGCCT TCATCACCTT CTCCAACCCC ATCACCACCC 
CCAGTCTTGG AACCGGGGTC TGAGCCTAAT CTCGCAGTCC TCTCTGTTCC TGGGGACACA 
ATGACAACAG GGATGATACA GACGTCTGTA GAAGAATCAA CCCCCACGCC CCCTGAAAGT 
GGGGAGCCGT GTTGCCTCTC TCCAGAACCC ACTCCCCTTG CTGAACCCAT ATTGGAAGTA 
GAAGTGACGC TGAGCAAACC AGTTCCAGAA TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT 
CCCAGCCCCC TGGCATCTCA CAAGGTGGAA ATTCTTCCTG AGCCTAATGG CACAGTCCCA 
TCTGAGAATT TGGAACCAGA GGTGGAGTCA AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA 
GCTTGTCCCT CTGAATCCCC CATGCCTATT GCTCCAACTG CCCAACCTGA GGAACTACTC 
AACGGAGCCC CTTCACCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAACC AGAGGAACAG 
GCCAAGGAGG CTATAGTGTC GGTGACTCCC CCCACTGTCC TTTCTGCTAC CCCAGCTGTG 
GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG GAGGACATGG AGGAGGAGGA AGAAGAGGAA 
GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA GGAGAGGAAC CTCTCCCCCA AGAGAGCACC 
CCTGTCCCAG CCCACCTATC CCAGAATTCG GAGGTGGCAG TAGCCACCCA AGTGGCAGTG 
TCTGTGCCAA AGAGGAGACG GAAAATTAAG GAGCTCAATA AGAAGGAGGC TGTAGGAGAC 
CTTCTAGATG CCTTCAAGGA GGCGAACCCA GGGGTACCAG AGGTGGAAAA TCAGCCTCCT 
GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA 
GGAGAGGAGA CCTGGGACTC AAAGGAAGAC AAGATTCAAA ATGCAGAGAA TATCCAGCCA 
GGGGAACAGA AGTATGAATA TAAGTCAGAT CAGTGGAAGC CTCTAAACCT TGAGGAGAAA 
AAGCGTTATG ACCGAGAGTT CCTGCTTGGC TTTCAGTTCA TCTTTGCCAG TATGCAGAAG 
CCTGAGGGAT TGCCCCACAT CAGTGATGTG GTGTTGGATA AGGCCAATAA AACACCATTG 
CGACCGCTGG ACCCCTCTAG ACTTCAGGGC ATAAATTGTG GCCCAGACTT CACTCCGTCT 
TTTGCCAACC TTGGCCGACC AGCCCTTAGC AACCGTGGGC CCCCGAGGGG TGGGCCAGGT 
GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG GGACCCCGGC GATCTCAGCA GGGCCCCCGA 
AAGGAACCAC GCAAGATCAT TGCCACAGTG TCAATGACCG AAGATATAAA GCTGAACAAA 
GCAGAAAAGG CCTGGAAACC CAGTAGCAAG CGGACGGCGG CTGATAAGGA CCGAGGAGAA 
GAGGATGCTG ATGGCAGCAA AACCCAGGAC CTGTTCCGCA GGGTGCGCTC CATCTTGAAT 
AAGCTGACAC CCCAGATGTT CCAGCAGCTG ATGAAGCAGG TGACGCAGCT AGCCATTGAT 
ACCGAGGAAC GTCTCAAAGG GGTCATTGAC CTCATCTTCG AGAAGGCCAT TTCAGAACCC 
AACTTCTCCG TGGCCTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT 
ACAGAAAAGC CAACAGTGAC TGTGAACTTC CGAAAACTGT TGTTAAATCG ATGTCAGAAG 
GAGTTTGAAA AAGACAAAGA TGATGATGAG GTTTTTGAGA AGAAGCAAAA AGAGATGGAT 
GAAGCTGCTA CGGCAGAGGA ACGGGGACGC CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC 
ATAGCCCGGC GGCGCTCGTT AGGGAATATC AAGTTTATCG GGGAGTTGTT CAAATTGAAG 
ATGTTAACAG AGGCAATCAT GCATGACTGT GTGGTTAAAC TACTTAAGAA CCATGATGAA 
GAGTCCCTTG AATGCCTTTG CCGTCTGCTC ACCACCATTG GCAAAGACCT GGACTTTGAG 
AAGGCCAAGC CCCGGATGGA TCAGTATTTC AACCAGATGG AAAAAATCAT TAAGGAAAAG 
AAGACTTCAT CCCGGATCCG CTTTATGCTG CAGGATGTGC TGGATCTGCG AAAGAGCAAC 
TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC AAGACAATTG ACCAAATCCA CAAGGAAGCT 
GAGATGGAGG AGCATCGGGA GCACATAAAA GTGCAGCAGC TAATGGCCAA GGGCAGCGAC 
AAGCGTCGGG GTGGCCCTCC AGGCCCACCC ATCAGCCGTG GCCTTCCACT TGTGGATGAT 
GGTGGCTGGA ACACAGTACC CATCAGCAAG GGCAGCCGCC CTATTGACAC TTCTCGACTC 
ACTAAGATCA CAAAGCCTGG CTCCATTGAT TCTAATAACC AGCTGTTTGC ACCTGGAGGC 
CGATTGAGCT GGGGCAAGGG TAGCAGTGGA GGCTCAGGAG CCAAGCCCTC CGATGCAGCA 
TCAGAAGCTG CTCGTCCAGC TACTAGTACC TTGAATCGCT TCTCAGCCCT TCAACAAGCA 
GTACCAACAG AAAGCACAGA CAACAGACGA GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA 
CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC CGCCTAGAGC GGAGTGAACG GGGAGGTGAC 
CGTGGTGACC GGCTTGATCG CGCCCGGACA CCCGCCACCA AGCGGAGCTT CAGCAAGGAA 
GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT CAGCCTGATG GACTACGCAA GGCAGCTAGC 
CTCACGGAGG ATCGGGACCG CGGGCGGGAA GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG 
GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA GAGGAGCTGG AGAAGAAATC CAGGGCCATC 
ATTGAGGAGT ACCTCCATCT CAATGACATG AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG 
GCTTCGCCCT CCCTGCTCTT TATCTTTGTA CGGCACGGCA TCGAGTCCAC GCTGGAGCGC 
AGCGCCATCA CGCGGGAGCA CATGGGCCGG CTGCTGCACC AGCTGCTCTG TGCTGGGCAC 
CTCTCCACTG CTCAGTACTA CCAAGGGCTA TATGAAATTC TAGAGTTGGC TGAAGACATG 
GAAATTGACA TCCCTCATGT GTGGCTCTAC CTAGCAGAAT TGATAACGCC CATTCTGCAG 
GAAGGTGGGG TGCCAATGGG GGAGCTGTTC AGGGAAATAA CAAAACCTCT GAGACCCCTG 
GGCAAGGCTG CTTCCCTGTT GCTGGAGATC CTGGGGCTCC TGTGCAAAAG CATGGGTCCT 
AAAAAGGTGG GGACGCTGTG GCGAGAGGCT GGACTCAGCT GGAAGGAATT TCTGCCTGAA 
GGCCAGGATG TCAGTGCCTT TGTCACTGCG CAGAAGGTGG AGTATACCCT GGGAGAGGAG 
TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG 
CTGCTGCAGG AGGGCAGCAG TAACCAGCGG GTGTTTGACT GGATAGAGGC CAACCTGAAC 
GAGCAGCAGG TGACATCCAA CACATTAGTT CGAGCCCTTA TGACAACTGT CTGCTATTCT 
GCAATTATCT TTGAGTCTTC TCTCCGAGTG GATGTTGCAG TGCTGAAAGC ACGAGCGAAA 
CTGCTACAGA AATACCTGTG TGACGAGCAG AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG 
GCCCTTGTAG TGACCTTAGA CCAGCCCCCT AACCTGCTTG GGATGTTCTT CGATGCGCTG 
TATGACGAGG ACGTGGTGAA GGAGGACGCC TTCTATAGCT GGGAGAGTAG CAAGGACCCT 
GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA AAATCTGTCA CGACTTTTTT CAAGTGGCTT 
CGTGAGGCGG AGGAGGAGGA GTCTGACCAC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_024995330.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851098.1 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa219177
Clone ID Related Accession (Same CDS sequence)	XM_024995330.1
Accession Version	XM_024995330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGC CGTTCCACAT ATGTTGTCCC GACACAGCAG 
TATCCTGTGC AGCCGGGAGC CCCAAGCTTC TACCCAGGTG CAAGCCCTAC AGAGTTTGGG 
ACCTACGCTG GCGCTTACTA CCCAGCCCAG GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT 
CCCCCACCTG TTTTGATGAA CCAGCCACCC CAGATTGCTC CCAAGAGGGA GCGGAAGACG 
ATCCGAATTC GAGACCCAAA CCAAGGAGGG AAGGATATCA CGGAGGAGAT CATGTCTGGG 
GCCCGCACTG CCTCCACACC CACACCTCCC CAGACGGGAG GCGGTCTGGA GCCTCAGGCT 
AATGGGGAGA CACCCCAGGT TGCTGTCGTC GTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851098.1
CDS	248..4876
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_024995330.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995330.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGC CGTTCCACAT ATGTTGTCCC GACACAGCAG 
TATCCTGTGC AGCCGGGAGC CCCAAGCTTC TACCCAGGTG CAAGCCCTAC AGAGTTTGGG 
ACCTACGCTG GCGCTTACTA CCCAGCCCAG GGTGTGCAGC AGTTTCCCAC TGGTGTAGCT 
CCCCCACCTG TTTTGATGAA CCAGCCACCC CAGATTGCTC CCAAGAGGGA GCGGAAGACG 
ATCCGAATTC GAGACCCAAA CCAAGGAGGG AAGGATATCA CGGAGGAGAT CATGTCTGGG 
GCCCGCACTG CCTCCACACC CACACCTCCC CAGACGGGAG GCGGTCTGGA GCCTCAGGCT 
AATGGGGAGA CACCCCAGGT TGCTGTCGTC GTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_024995332.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851100.1 Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA.

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CloneID	OBa219178
Clone ID Related Accession (Same CDS sequence)	XM_024995332.1
Accession Version	XM_024995332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
GGCCGTTCCA CATATGTTGT CCCGACACAG CAGTATCCTG TGCAGCCGGG AGCCCCAAGC 
TTCTACCCAG GTGCAAGCCC TACAGAGTTT GGGACCTACG CTGGCGCTTA CTACCCAGCC 
CAGGGTGTGC AGCAGTTTCC CACTGGTGTA GCTCCCCCAC CTGTTTTGAT GAACCAGCCA 
CCCCAGATTG CTCCCAAGAG GGAGCGGAAG ACGATCCGAA TTCGAGACCC AAACCAAGGA 
GGGAAGGATA TCACGGAGGA GATCATGTCT GGGGCCCGCA CTGCCTCCAC ACCCACACCT 
CCCCAGACGG GAGGCGGTCT GGAGCCTCAG GCTAATGGGG AGACACCCCA GGTTGCTGTC 
GTCGTCCGGC CAGATGACCG GTCGCAGGGA GCAATCATTG GGGAGCGGCC AGGGCTGCCT 
GGCCCAGAGC ACAGCCCTTC AGAATCCCAG CCTTCATCAC CTTCTCCAAC CCCATCACCA 
CCCCCAGTCT TGGAACCGGG GTCTGAGCCT AATCTCGCAG TCCTCTCTGT TCCTGGGGAC 
ACAATGACAA CAGGGATGAT ACAGACGTCT GTAGAAGAAT CAACCCCCAC GCCCCCTGAA 
AGTGGGGAGC CGTGTTGCCT CTCTCCAGAA CCCACTCCCC TTGCTGAACC CATATTGGAA 
GTAGAAGTGA CGCTGAGCAA ACCAGTTCCA GAATCTGAGT TCTCTTCCAG TCCTCTCCAG 
GTTCCCAGCC CCCTGGCATC TCACAAGGTG GAAATTCTTC CTGAGCCTAA TGGCACAGTC 
CCATCTGAGA ATTTGGAACC AGAGGTGGAG TCAAGCCCAG AGCTTGCCCC TCTCCCTCCT 
CCAGCTTGTC CCTCTGAATC CCCCATGCCT ATTGCTCCAA CTGCCCAACC TGAGGAACTA 
CTCAACGGAG CCCCTTCACC ACCAGCTGTG GACTTAAGCC CAGTCAGTGA ACCAGAGGAA 
CAGGCCAAGG AGGCTATAGT GTCGGTGACT CCCCCCACTG TCCTTTCTGC TACCCCAGCT 
GTGGCTCCTC CAGTGGCTTC CCCTGCTCAG GAGGAGGACA TGGAGGAGGA GGAAGAAGAG 
GAAGAGGAAG GAGAAGCTGA GGGTGAGAAG GGAGGAGAGG AACCTCTCCC CCAAGAGAGC 
ACCCCTGTCC CAGCCCACCT ATCCCAGAAT TCGGAGGTGG CAGTAGCCAC CCAAGTGGCA 
GTGTCTGTGC CAAAGAGGAG ACGGAAAATT AAGGAGCTCA ATAAGAAGGA GGCTGTAGGA 
GACCTTCTAG ATGCCTTCAA GGAGGCGAAC CCAGGGGTAC CAGAGGTGGA AAATCAGCCT 
CCTGCAGGCT GCAATCCCAG CCCAGAGCCT GAGGGCAGCA GTGTGCCCCT GCGGTCTGAG 
GAAGGAGAGG AGACCTGGGA CTCAAAGGAA GACAAGATTC AAAATGCAGA GAATATCCAG 
CCAGGGGAAC AGAAGTATGA ATATAAGTCA GATCAGTGGA AGCCTCTAAA CCTTGAGGAG 
AAAAAGCGTT ATGACCGAGA GTTCCTGCTT GGCTTTCAGT TCATCTTTGC CAGTATGCAG 
AAGCCTGAGG GATTGCCCCA CATCAGTGAT GTGGTGTTGG ATAAGGCCAA TAAAACACCA 
TTGCGACCGC TGGACCCCTC TAGACTTCAG GGCATAAATT GTGGCCCAGA CTTCACTCCG 
TCTTTTGCCA ACCTTGGCCG ACCAGCCCTT AGCAACCGTG GGCCCCCGAG GGGTGGGCCA 
GGTGGGGAGC TGCCCCGAGG GCCGCAGGCT GGCCTGGGAC CCCGGCGATC TCAGCAGGGC 
CCCCGAAAGG AACCACGCAA GATCATTGCC ACAGTGTCAA TGACCGAAGA TATAAAGCTG 
AACAAAGCAG AAAAGGCCTG GAAACCCAGT AGCAAGCGGA CGGCGGCTGA TAAGGACCGA 
GGAGAAGAGG ATGCTGATGG CAGCAAAACC CAGGACCTGT TCCGCAGGGT GCGCTCCATC 
TTGAATAAGC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAGGTGAC GCAGCTAGCC 
ATTGATACCG AGGAACGTCT CAAAGGGGTC ATTGACCTCA TCTTCGAGAA GGCCATTTCA 
GAACCCAACT TCTCCGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG 
CCCACTACAG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AACTGTTGTT AAATCGATGT 
CAGAAGGAGT TTGAAAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG 
ATGGATGAAG CTGCTACGGC AGAGGAACGG GGACGCCTGA AAGAAGAGCT GGAAGAGGCT 
CGAGACATAG CCCGGCGGCG CTCGTTAGGG AATATCAAGT TTATCGGGGA GTTGTTCAAA 
TTGAAGATGT TAACAGAGGC AATCATGCAT GACTGTGTGG TTAAACTACT TAAGAACCAT 
GATGAAGAGT CCCTTGAATG CCTTTGCCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC 
TTTGAGAAGG CCAAGCCCCG GATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAG 
GAAAAGAAGA CTTCATCCCG GATCCGCTTT ATGCTGCAGG ATGTGCTGGA TCTGCGAAAG 
AGCAACTGGG TGCCACGCCG TGGGGACCAG GGGCCCAAGA CAATTGACCA AATCCACAAG 
GAAGCTGAGA TGGAGGAGCA TCGGGAGCAC ATAAAAGTGC AGCAGCTAAT GGCCAAGGGC 
AGCGACAAGC GTCGGGGTGG CCCTCCAGGC CCACCCATCA GCCGTGGCCT TCCACTTGTG 
GATGATGGTG GCTGGAACAC AGTACCCATC AGCAAGGGCA GCCGCCCTAT TGACACTTCT 
CGACTCACTA AGATCACAAA GCCTGGCTCC ATTGATTCTA ATAACCAGCT GTTTGCACCT 
GGAGGCCGAT TGAGCTGGGG CAAGGGTAGC AGTGGAGGCT CAGGAGCCAA GCCCTCCGAT 
GCAGCATCAG AAGCTGCTCG TCCAGCTACT AGTACCTTGA ATCGCTTCTC AGCCCTTCAA 
CAAGCAGTAC CAACAGAAAG CACAGACAAC AGACGAGTGG TGCAGAGGAG TAGCTTGAGC 
CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGCC TAGAGCGGAG TGAACGGGGA 
GGTGACCGTG GTGACCGGCT TGATCGCGCC CGGACACCCG CCACCAAGCG GAGCTTCAGC 
AAGGAAGTGG AGGAACGGAG TAGAGAGCGG CCCTCTCAGC CTGATGGACT ACGCAAGGCA 
GCTAGCCTCA CGGAGGATCG GGACCGCGGG CGGGAAGCTG TGAAACGAGA AGCTGCCCTC 
CCCCCGGTGA GTCCCCCGAA GGCTGCACTC TCTGAAGAGG AGCTGGAGAA GAAATCCAGG 
GCCATCATTG AGGAGTACCT CCATCTCAAT GACATGAAGG AGGCAGTGCA GTGCGTGCAG 
GAGCTGGCTT CGCCCTCCCT GCTCTTTATC TTTGTACGGC ACGGCATCGA GTCCACGCTG 
GAGCGCAGCG CCATCACGCG GGAGCACATG GGCCGGCTGC TGCACCAGCT GCTCTGTGCT 
GGGCACCTCT CCACTGCTCA GTACTACCAA GGGCTATATG AAATTCTAGA GTTGGCTGAA 
GACATGGAAA TTGACATCCC TCATGTGTGG CTCTACCTAG CAGAATTGAT AACGCCCATT 
CTGCAGGAAG GTGGGGTGCC AATGGGGGAG CTGTTCAGGG AAATAACAAA ACCTCTGAGA 
CCCCTGGGCA AGGCTGCTTC CCTGTTGCTG GAGATCCTGG GGCTCCTGTG CAAAAGCATG 
GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAGGCTGGAC TCAGCTGGAA GGAATTTCTG 
CCTGAAGGCC AGGATGTCAG TGCCTTTGTC ACTGCGCAGA AGGTGGAGTA TACCCTGGGA 
GAGGAGTTAG AAGCCCCAGG CCAGAGGTTG CTGTCCTTTG AGGAGCTGCG CAGGCAGCTG 
GAGAAGCTGC TGCAGGAGGG CAGCAGTAAC CAGCGGGTGT TTGACTGGAT AGAGGCCAAC 
CTGAACGAGC AGCAGGTGAC ATCCAACACA TTAGTTCGAG CCCTTATGAC AACTGTCTGC 
TATTCTGCAA TTATCTTTGA GTCTTCTCTC CGAGTGGATG TTGCAGTGCT GAAAGCACGA 
GCGAAACTGC TACAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTGCAGGC GCTCTATGCC 
CTCCAGGCCC TTGTAGTGAC CTTAGACCAG CCCCCTAACC TGCTTGGGAT GTTCTTCGAT 
GCGCTGTATG ACGAGGACGT GGTGAAGGAG GACGCCTTCT ATAGCTGGGA GAGTAGCAAG 
GACCCTGCTG AGCAGCAGGG CAAGGGCGTG GCCCTAAAAT CTGTCACGAC TTTTTTCAAG 
TGGCTTCGTG AGGCGGAGGA GGAGGAGTCT GACCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851100.1
CDS	248..4789
Translation	MNKAPQPTGPPPAPSPGLPQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVVRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_024995332.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995332.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
GGCCGTTCCA CATATGTTGT CCCGACACAG CAGTATCCTG TGCAGCCGGG AGCCCCAAGC 
TTCTACCCAG GTGCAAGCCC TACAGAGTTT GGGACCTACG CTGGCGCTTA CTACCCAGCC 
CAGGGTGTGC AGCAGTTTCC CACTGGTGTA GCTCCCCCAC CTGTTTTGAT GAACCAGCCA 
CCCCAGATTG CTCCCAAGAG GGAGCGGAAG ACGATCCGAA TTCGAGACCC AAACCAAGGA 
GGGAAGGATA TCACGGAGGA GATCATGTCT GGGGCCCGCA CTGCCTCCAC ACCCACACCT 
CCCCAGACGG GAGGCGGTCT GGAGCCTCAG GCTAATGGGG AGACACCCCA GGTTGCTGTC 
GTCGTCCGGC CAGATGACCG GTCGCAGGGA GCAATCATTG GGGAGCGGCC AGGGCTGCCT 
GGCCCAGAGC ACAGCCCTTC AGAATCCCAG CCTTCATCAC CTTCTCCAAC CCCATCACCA 
CCCCCAGTCT TGGAACCGGG GTCTGAGCCT AATCTCGCAG TCCTCTCTGT TCCTGGGGAC 
ACAATGACAA CAGGGATGAT ACAGACGTCT GTAGAAGAAT CAACCCCCAC GCCCCCTGAA 
AGTGGGGAGC CGTGTTGCCT CTCTCCAGAA CCCACTCCCC TTGCTGAACC CATATTGGAA 
GTAGAAGTGA CGCTGAGCAA ACCAGTTCCA GAATCTGAGT TCTCTTCCAG TCCTCTCCAG 
GTTCCCAGCC CCCTGGCATC TCACAAGGTG GAAATTCTTC CTGAGCCTAA TGGCACAGTC 
CCATCTGAGA ATTTGGAACC AGAGGTGGAG TCAAGCCCAG AGCTTGCCCC TCTCCCTCCT 
CCAGCTTGTC CCTCTGAATC CCCCATGCCT ATTGCTCCAA CTGCCCAACC TGAGGAACTA 
CTCAACGGAG CCCCTTCACC ACCAGCTGTG GACTTAAGCC CAGTCAGTGA ACCAGAGGAA 
CAGGCCAAGG AGGCTATAGT GTCGGTGACT CCCCCCACTG TCCTTTCTGC TACCCCAGCT 
GTGGCTCCTC CAGTGGCTTC CCCTGCTCAG GAGGAGGACA TGGAGGAGGA GGAAGAAGAG 
GAAGAGGAAG GAGAAGCTGA GGGTGAGAAG GGAGGAGAGG AACCTCTCCC CCAAGAGAGC 
ACCCCTGTCC CAGCCCACCT ATCCCAGAAT TCGGAGGTGG CAGTAGCCAC CCAAGTGGCA 
GTGTCTGTGC CAAAGAGGAG ACGGAAAATT AAGGAGCTCA ATAAGAAGGA GGCTGTAGGA 
GACCTTCTAG ATGCCTTCAA GGAGGCGAAC CCAGGGGTAC CAGAGGTGGA AAATCAGCCT 
CCTGCAGGCT GCAATCCCAG CCCAGAGCCT GAGGGCAGCA GTGTGCCCCT GCGGTCTGAG 
GAAGGAGAGG AGACCTGGGA CTCAAAGGAA GACAAGATTC AAAATGCAGA GAATATCCAG 
CCAGGGGAAC AGAAGTATGA ATATAAGTCA GATCAGTGGA AGCCTCTAAA CCTTGAGGAG 
AAAAAGCGTT ATGACCGAGA GTTCCTGCTT GGCTTTCAGT TCATCTTTGC CAGTATGCAG 
AAGCCTGAGG GATTGCCCCA CATCAGTGAT GTGGTGTTGG ATAAGGCCAA TAAAACACCA 
TTGCGACCGC TGGACCCCTC TAGACTTCAG GGCATAAATT GTGGCCCAGA CTTCACTCCG 
TCTTTTGCCA ACCTTGGCCG ACCAGCCCTT AGCAACCGTG GGCCCCCGAG GGGTGGGCCA 
GGTGGGGAGC TGCCCCGAGG GCCGCAGGCT GGCCTGGGAC CCCGGCGATC TCAGCAGGGC 
CCCCGAAAGG AACCACGCAA GATCATTGCC ACAGTGTCAA TGACCGAAGA TATAAAGCTG 
AACAAAGCAG AAAAGGCCTG GAAACCCAGT AGCAAGCGGA CGGCGGCTGA TAAGGACCGA 
GGAGAAGAGG ATGCTGATGG CAGCAAAACC CAGGACCTGT TCCGCAGGGT GCGCTCCATC 
TTGAATAAGC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAGGTGAC GCAGCTAGCC 
ATTGATACCG AGGAACGTCT CAAAGGGGTC ATTGACCTCA TCTTCGAGAA GGCCATTTCA 
GAACCCAACT TCTCCGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG 
CCCACTACAG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AACTGTTGTT AAATCGATGT 
CAGAAGGAGT TTGAAAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG 
ATGGATGAAG CTGCTACGGC AGAGGAACGG GGACGCCTGA AAGAAGAGCT GGAAGAGGCT 
CGAGACATAG CCCGGCGGCG CTCGTTAGGG AATATCAAGT TTATCGGGGA GTTGTTCAAA 
TTGAAGATGT TAACAGAGGC AATCATGCAT GACTGTGTGG TTAAACTACT TAAGAACCAT 
GATGAAGAGT CCCTTGAATG CCTTTGCCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC 
TTTGAGAAGG CCAAGCCCCG GATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAG 
GAAAAGAAGA CTTCATCCCG GATCCGCTTT ATGCTGCAGG ATGTGCTGGA TCTGCGAAAG 
AGCAACTGGG TGCCACGCCG TGGGGACCAG GGGCCCAAGA CAATTGACCA AATCCACAAG 
GAAGCTGAGA TGGAGGAGCA TCGGGAGCAC ATAAAAGTGC AGCAGCTAAT GGCCAAGGGC 
AGCGACAAGC GTCGGGGTGG CCCTCCAGGC CCACCCATCA GCCGTGGCCT TCCACTTGTG 
GATGATGGTG GCTGGAACAC AGTACCCATC AGCAAGGGCA GCCGCCCTAT TGACACTTCT 
CGACTCACTA AGATCACAAA GCCTGGCTCC ATTGATTCTA ATAACCAGCT GTTTGCACCT 
GGAGGCCGAT TGAGCTGGGG CAAGGGTAGC AGTGGAGGCT CAGGAGCCAA GCCCTCCGAT 
GCAGCATCAG AAGCTGCTCG TCCAGCTACT AGTACCTTGA ATCGCTTCTC AGCCCTTCAA 
CAAGCAGTAC CAACAGAAAG CACAGACAAC AGACGAGTGG TGCAGAGGAG TAGCTTGAGC 
CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGCC TAGAGCGGAG TGAACGGGGA 
GGTGACCGTG GTGACCGGCT TGATCGCGCC CGGACACCCG CCACCAAGCG GAGCTTCAGC 
AAGGAAGTGG AGGAACGGAG TAGAGAGCGG CCCTCTCAGC CTGATGGACT ACGCAAGGCA 
GCTAGCCTCA CGGAGGATCG GGACCGCGGG CGGGAAGCTG TGAAACGAGA AGCTGCCCTC 
CCCCCGGTGA GTCCCCCGAA GGCTGCACTC TCTGAAGAGG AGCTGGAGAA GAAATCCAGG 
GCCATCATTG AGGAGTACCT CCATCTCAAT GACATGAAGG AGGCAGTGCA GTGCGTGCAG 
GAGCTGGCTT CGCCCTCCCT GCTCTTTATC TTTGTACGGC ACGGCATCGA GTCCACGCTG 
GAGCGCAGCG CCATCACGCG GGAGCACATG GGCCGGCTGC TGCACCAGCT GCTCTGTGCT 
GGGCACCTCT CCACTGCTCA GTACTACCAA GGGCTATATG AAATTCTAGA GTTGGCTGAA 
GACATGGAAA TTGACATCCC TCATGTGTGG CTCTACCTAG CAGAATTGAT AACGCCCATT 
CTGCAGGAAG GTGGGGTGCC AATGGGGGAG CTGTTCAGGG AAATAACAAA ACCTCTGAGA 
CCCCTGGGCA AGGCTGCTTC CCTGTTGCTG GAGATCCTGG GGCTCCTGTG CAAAAGCATG 
GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAGGCTGGAC TCAGCTGGAA GGAATTTCTG 
CCTGAAGGCC AGGATGTCAG TGCCTTTGTC ACTGCGCAGA AGGTGGAGTA TACCCTGGGA 
GAGGAGTTAG AAGCCCCAGG CCAGAGGTTG CTGTCCTTTG AGGAGCTGCG CAGGCAGCTG 
GAGAAGCTGC TGCAGGAGGG CAGCAGTAAC CAGCGGGTGT TTGACTGGAT AGAGGCCAAC 
CTGAACGAGC AGCAGGTGAC ATCCAACACA TTAGTTCGAG CCCTTATGAC AACTGTCTGC 
TATTCTGCAA TTATCTTTGA GTCTTCTCTC CGAGTGGATG TTGCAGTGCT GAAAGCACGA 
GCGAAACTGC TACAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTGCAGGC GCTCTATGCC 
CTCCAGGCCC TTGTAGTGAC CTTAGACCAG CCCCCTAACC TGCTTGGGAT GTTCTTCGAT 
GCGCTGTATG ACGAGGACGT GGTGAAGGAG GACGCCTTCT ATAGCTGGGA GAGTAGCAAG 
GACCCTGCTG AGCAGCAGGG CAAGGGCGTG GCCCTAAAAT CTGTCACGAC TTTTTTCAAG 
TGGCTTCGTG AGGCGGAGGA GGAGGAGTCT GACCACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_010801318.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799620.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

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CloneID	OBa45811
Clone ID Related Accession (Same CDS sequence)	XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
Accession Version	XM_010801318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799620.1
CDS	637..4785
Translation	MGRHPRLLSSLRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_010801318.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801318.1

ORF Insert Sequence:

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_015462063.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317549.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45811
Clone ID Related Accession (Same CDS sequence)	XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
Accession Version	XM_015462063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317549.1
CDS	876..5024
Translation	MGRHPRLLSSLRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_015462063.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462063.1

ORF Insert Sequence:

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_015462064.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317550.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45811
Clone ID Related Accession (Same CDS sequence)	XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
Accession Version	XM_015462064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317550.1
CDS	637..4785
Translation	MGRHPRLLSSLRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_015462064.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462064.1

ORF Insert Sequence:

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_015472316.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327802.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

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CloneID	OBa45811
Clone ID Related Accession (Same CDS sequence)	XM_010801318.1 , XM_015462063.1 , XM_015462064.1 , XM_015472316.1
Accession Version	XM_015472316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327802.1
CDS	876..5024
Translation	MGRHPRLLSSLRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_015472316.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472316.1

ORF Insert Sequence:

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GCAGGCTGGC 
CTGGGACCCC GGCGATCTCA GCAGGGCCCC CGAAAGGAAC CACGCAAGAT CATTGCCACA 
GTGTCAATGA CCGAAGATAT AAAGCTGAAC AAAGCAGAAA AGGCCTGGAA ACCCAGTAGC 
AAGCGGACGG CGGCTGATAA GGACCGAGGA GAAGAGGATG CTGATGGCAG CAAAACCCAG 
GACCTGTTCC GCAGGGTGCG CTCCATCTTG AATAAGCTGA CACCCCAGAT GTTCCAGCAG 
CTGATGAAGC AGGTGACGCA GCTAGCCATT GATACCGAGG AACGTCTCAA AGGGGTCATT 
GACCTCATCT TCGAGAAGGC CATTTCAGAA CCCAACTTCT CCGTGGCCTA TGCCAACATG 
TGCCGCTGCC TCATGGCGCT GAAAGTGCCC ACTACAGAAA AGCCAACAGT GACTGTGAAC 
TTCCGAAAAC TGTTGTTAAA TCGATGTCAG AAGGAGTTTG AAAAAGACAA AGATGATGAT 
GAGGTTTTTG AGAAGAAGCA AAAAGAGATG GATGAAGCTG CTACGGCAGA GGAACGGGGA 
CGCCTGAAAG AAGAGCTGGA AGAGGCTCGA GACATAGCCC GGCGGCGCTC GTTAGGGAAT 
ATCAAGTTTA TCGGGGAGTT GTTCAAATTG AAGATGTTAA CAGAGGCAAT CATGCATGAC 
TGTGTGGTTA AACTACTTAA GAACCATGAT GAAGAGTCCC TTGAATGCCT TTGCCGTCTG 
CTCACCACCA TTGGCAAAGA CCTGGACTTT GAGAAGGCCA AGCCCCGGAT GGATCAGTAT 
TTCAACCAGA TGGAAAAAAT CATTAAGGAA AAGAAGACTT CATCCCGGAT CCGCTTTATG 
CTGCAGGATG TGCTGGATCT GCGAAAGAGC AACTGGGTGC CACGCCGTGG GGACCAGGGG 
CCCAAGACAA TTGACCAAAT CCACAAGGAA GCTGAGATGG AGGAGCATCG GGAGCACATA 
AAAGTGCAGC AGCTAATGGC CAAGGGCAGC GACAAGCGTC GGGGTGGCCC TCCAGGCCCA 
CCCATCAGCC GTGGCCTTCC ACTTGTGGAT GATGGTGGCT GGAACACAGT ACCCATCAGC 
AAGGGCAGCC GCCCTATTGA CACTTCTCGA CTCACTAAGA TCACAAAGCC TGGCTCCATT 
GATTCTAATA ACCAGCTGTT TGCACCTGGA GGCCGATTGA GCTGGGGCAA GGGTAGCAGT 
GGAGGCTCAG GAGCCAAGCC CTCCGATGCA GCATCAGAAG CTGCTCGTCC AGCTACTAGT 
ACCTTGAATC GCTTCTCAGC CCTTCAACAA GCAGTACCAA CAGAAAGCAC AGACAACAGA 
CGAGTGGTGC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA 
GACCGCCTAG AGCGGAGTGA ACGGGGAGGT GACCGTGGTG ACCGGCTTGA TCGCGCCCGG 
ACACCCGCCA CCAAGCGGAG CTTCAGCAAG GAAGTGGAGG AACGGAGTAG AGAGCGGCCC 
TCTCAGCCTG ATGGACTACG CAAGGCAGCT AGCCTCACGG AGGATCGGGA CCGCGGGCGG 
GAAGCTGTGA AACGAGAAGC TGCCCTCCCC CCGGTGAGTC CCCCGAAGGC TGCACTCTCT 
GAAGAGGAGC TGGAGAAGAA ATCCAGGGCC ATCATTGAGG AGTACCTCCA TCTCAATGAC 
ATGAAGGAGG CAGTGCAGTG CGTGCAGGAG CTGGCTTCGC CCTCCCTGCT CTTTATCTTT 
GTACGGCACG GCATCGAGTC CACGCTGGAG CGCAGCGCCA TCACGCGGGA GCACATGGGC 
CGGCTGCTGC ACCAGCTGCT CTGTGCTGGG CACCTCTCCA CTGCTCAGTA CTACCAAGGG 
CTATATGAAA TTCTAGAGTT GGCTGAAGAC ATGGAAATTG ACATCCCTCA TGTGTGGCTC 
TACCTAGCAG AATTGATAAC GCCCATTCTG CAGGAAGGTG GGGTGCCAAT GGGGGAGCTG 
TTCAGGGAAA TAACAAAACC TCTGAGACCC CTGGGCAAGG CTGCTTCCCT GTTGCTGGAG 
ATCCTGGGGC TCCTGTGCAA AAGCATGGGT CCTAAAAAGG TGGGGACGCT GTGGCGAGAG 
GCTGGACTCA GCTGGAAGGA ATTTCTGCCT GAAGGCCAGG ATGTCAGTGC CTTTGTCACT 
GCGCAGAAGG TGGAGTATAC CCTGGGAGAG GAGTTAGAAG CCCCAGGCCA GAGGTTGCTG 
TCCTTTGAGG AGCTGCGCAG GCAGCTGGAG AAGCTGCTGC AGGAGGGCAG CAGTAACCAG 
CGGGTGTTTG ACTGGATAGA GGCCAACCTG AACGAGCAGC AGGTGACATC CAACACATTA 
GTTCGAGCCC TTATGACAAC TGTCTGCTAT TCTGCAATTA TCTTTGAGTC TTCTCTCCGA 
GTGGATGTTG CAGTGCTGAA AGCACGAGCG AAACTGCTAC AGAAATACCT GTGTGACGAG 
CAGAAGGAGC TGCAGGCGCT CTATGCCCTC CAGGCCCTTG TAGTGACCTT AGACCAGCCC 
CCTAACCTGC TTGGGATGTT CTTCGATGCG CTGTATGACG AGGACGTGGT GAAGGAGGAC 
GCCTTCTATA GCTGGGAGAG TAGCAAGGAC CCTGCTGAGC AGCAGGGCAA GGGCGTGGCC 
CTAAAATCTG TCACGACTTT TTTCAAGTGG CTTCGTGAGG CGGAGGAGGA GGAGTCTGAC 
CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_010801319.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799621.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

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CloneID	OBa45812
Clone ID Related Accession (Same CDS sequence)	XM_010801319.2 , XM_015462065.1
Accession Version	XM_010801319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG 
GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG 
TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG 
CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC 
CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC 
CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC 
CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC 
AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT 
GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG 
CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC 
AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA 
GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC 
ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG 
GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT 
TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA 
GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC 
TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA 
GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA 
CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT 
CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA 
GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA 
GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG 
AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA 
CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA 
TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC 
CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC 
AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT 
GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC 
TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG 
GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT 
CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG 
GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT 
AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC 
TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA 
AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799621.1
CDS	876..5021
Translation	MGRHPRLLSSLRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_010801319.2
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801319.2

ORF Insert Sequence:

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG 
GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG 
TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG 
CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC 
CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC 
CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC 
CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC 
AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT 
GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG 
CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC 
AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA 
GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC 
ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG 
GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT 
TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA 
GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC 
TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA 
GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA 
CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT 
CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA 
GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA 
GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG 
AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA 
CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA 
TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC 
CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC 
AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT 
GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC 
TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG 
GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT 
CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG 
GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT 
AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC 
TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA 
AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_015462065.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317551.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45812
Clone ID Related Accession (Same CDS sequence)	XM_010801319.2 , XM_015462065.1
Accession Version	XM_015462065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG 
GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG 
TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG 
CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC 
CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC 
CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC 
CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC 
AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT 
GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG 
CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC 
AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA 
GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC 
ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG 
GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT 
TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA 
GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC 
TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA 
GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA 
CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT 
CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA 
GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA 
GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG 
AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA 
CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA 
TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC 
CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC 
AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT 
GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC 
TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG 
GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT 
CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG 
GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT 
AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC 
TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA 
AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317551.1
CDS	876..5021
Translation	MGRHPRLLSSLRPDDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_015462065.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462065.1

ORF Insert Sequence:

ATGGGGAGAC ACCCCAGGTT GCTGTCGTCC CTCCGGCCAG ATGACCGGTC GCAGGGAGCA 
ATCATTGGGG AGCGGCCAGG GCTGCCTGGC CCAGAGCACA GCCCTTCAGA ATCCCAGCCT 
TCATCACCTT CTCCAACCCC ATCACCACCC CCAGTCTTGG AACCGGGGTC TGAGCCTAAT 
CTCGCAGTCC TCTCTGTTCC TGGGGACACA ATGACAACAG GGATGATACA GACGTCTGTA 
GAAGAATCAA CCCCCACGCC CCCTGAAAGT GGGGAGCCGT GTTGCCTCTC TCCAGAACCC 
ACTCCCCTTG CTGAACCCAT ATTGGAAGTA GAAGTGACGC TGAGCAAACC AGTTCCAGAA 
TCTGAGTTCT CTTCCAGTCC TCTCCAGGTT CCCAGCCCCC TGGCATCTCA CAAGGTGGAA 
ATTCTTCCTG AGCCTAATGG CACAGTCCCA TCTGAGAATT TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCCCCTCT CCCTCCTCCA GCTTGTCCCT CTGAATCCCC CATGCCTATT 
GCTCCAACTG CCCAACCTGA GGAACTACTC AACGGAGCCC CTTCACCACC AGCTGTGGAC 
TTAAGCCCAG TCAGTGAACC AGAGGAACAG GCCAAGGAGG CTATAGTGTC GGTGACTCCC 
CCCACTGTCC TTTCTGCTAC CCCAGCTGTG GCTCCTCCAG TGGCTTCCCC TGCTCAGGAG 
GAGGACATGG AGGAGGAGGA AGAAGAGGAA GAGGAAGGAG AAGCTGAGGG TGAGAAGGGA 
GGAGAGGAAC CTCTCCCCCA AGAGAGCACC CCTGTCCCAG CCCACCTATC CCAGAATTCG 
GAGGTGGCAG TAGCCACCCA AGTGGCAGTG TCTGTGCCAA AGAGGAGACG GAAAATTAAG 
GAGCTCAATA AGAAGGAGGC TGTAGGAGAC CTTCTAGATG CCTTCAAGGA GGCGAACCCA 
GGGGTACCAG AGGTGGAAAA TCAGCCTCCT GCAGGCTGCA ATCCCAGCCC AGAGCCTGAG 
GGCAGCAGTG TGCCCCTGCG GTCTGAGGAA GGAGAGGAGA CCTGGGACTC AAAGGAAGAC 
AAGATTCAAA ATGCAGAGAA TATCCAGCCA GGGGAACAGA AGTATGAATA TAAGTCAGAT 
CAGTGGAAGC CTCTAAACCT TGAGGAGAAA AAGCGTTATG ACCGAGAGTT CCTGCTTGGC 
TTTCAGTTCA TCTTTGCCAG TATGCAGAAG CCTGAGGGAT TGCCCCACAT CAGTGATGTG 
GTGTTGGATA AGGCCAATAA AACACCATTG CGACCGCTGG ACCCCTCTAG ACTTCAGGGC 
ATAAATTGTG GCCCAGACTT CACTCCGTCT TTTGCCAACC TTGGCCGACC AGCCCTTAGC 
AACCGTGGGC CCCCGAGGGG TGGGCCAGGT GGGGAGCTGC CCCGAGGGCC GGCTGGCCTG 
GGACCCCGGC GATCTCAGCA GGGCCCCCGA AAGGAACCAC GCAAGATCAT TGCCACAGTG 
TCAATGACCG AAGATATAAA GCTGAACAAA GCAGAAAAGG CCTGGAAACC CAGTAGCAAG 
CGGACGGCGG CTGATAAGGA CCGAGGAGAA GAGGATGCTG ATGGCAGCAA AACCCAGGAC 
CTGTTCCGCA GGGTGCGCTC CATCTTGAAT AAGCTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAGG TGACGCAGCT AGCCATTGAT ACCGAGGAAC GTCTCAAAGG GGTCATTGAC 
CTCATCTTCG AGAAGGCCAT TTCAGAACCC AACTTCTCCG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAACTGT TGTTAAATCG ATGTCAGAAG GAGTTTGAAA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGGGGACGC 
CTGAAAGAAG AGCTGGAAGA GGCTCGAGAC ATAGCCCGGC GGCGCTCGTT AGGGAATATC 
AAGTTTATCG GGGAGTTGTT CAAATTGAAG ATGTTAACAG AGGCAATCAT GCATGACTGT 
GTGGTTAAAC TACTTAAGAA CCATGATGAA GAGTCCCTTG AATGCCTTTG CCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAG AAGGCCAAGC CCCGGATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAGGAAAAG AAGACTTCAT CCCGGATCCG CTTTATGCTG 
CAGGATGTGC TGGATCTGCG AAAGAGCAAC TGGGTGCCAC GCCGTGGGGA CCAGGGGCCC 
AAGACAATTG ACCAAATCCA CAAGGAAGCT GAGATGGAGG AGCATCGGGA GCACATAAAA 
GTGCAGCAGC TAATGGCCAA GGGCAGCGAC AAGCGTCGGG GTGGCCCTCC AGGCCCACCC 
ATCAGCCGTG GCCTTCCACT TGTGGATGAT GGTGGCTGGA ACACAGTACC CATCAGCAAG 
GGCAGCCGCC CTATTGACAC TTCTCGACTC ACTAAGATCA CAAAGCCTGG CTCCATTGAT 
TCTAATAACC AGCTGTTTGC ACCTGGAGGC CGATTGAGCT GGGGCAAGGG TAGCAGTGGA 
GGCTCAGGAG CCAAGCCCTC CGATGCAGCA TCAGAAGCTG CTCGTCCAGC TACTAGTACC 
TTGAATCGCT TCTCAGCCCT TCAACAAGCA GTACCAACAG AAAGCACAGA CAACAGACGA 
GTGGTGCAGA GGAGTAGCTT GAGCCGGGAA CGAGGTGAGA AAGCTGGGGA CCGGGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGTGAC CGTGGTGACC GGCTTGATCG CGCCCGGACA 
CCCGCCACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAAC GGAGTAGAGA GCGGCCCTCT 
CAGCCTGATG GACTACGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG CGGGCGGGAA 
GCTGTGAAAC GAGAAGCTGC CCTCCCCCCG GTGAGTCCCC CGAAGGCTGC ACTCTCTGAA 
GAGGAGCTGG AGAAGAAATC CAGGGCCATC ATTGAGGAGT ACCTCCATCT CAATGACATG 
AAGGAGGCAG TGCAGTGCGT GCAGGAGCTG GCTTCGCCCT CCCTGCTCTT TATCTTTGTA 
CGGCACGGCA TCGAGTCCAC GCTGGAGCGC AGCGCCATCA CGCGGGAGCA CATGGGCCGG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAC CTCTCCACTG CTCAGTACTA CCAAGGGCTA 
TATGAAATTC TAGAGTTGGC TGAAGACATG GAAATTGACA TCCCTCATGT GTGGCTCTAC 
CTAGCAGAAT TGATAACGCC CATTCTGCAG GAAGGTGGGG TGCCAATGGG GGAGCTGTTC 
AGGGAAATAA CAAAACCTCT GAGACCCCTG GGCAAGGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGGCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAGGCT 
GGACTCAGCT GGAAGGAATT TCTGCCTGAA GGCCAGGATG TCAGTGCCTT TGTCACTGCG 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TTAGAAGCCC CAGGCCAGAG GTTGCTGTCC 
TTTGAGGAGC TGCGCAGGCA GCTGGAGAAG CTGCTGCAGG AGGGCAGCAG TAACCAGCGG 
GTGTTTGACT GGATAGAGGC CAACCTGAAC GAGCAGCAGG TGACATCCAA CACATTAGTT 
CGAGCCCTTA TGACAACTGT CTGCTATTCT GCAATTATCT TTGAGTCTTC TCTCCGAGTG 
GATGTTGCAG TGCTGAAAGC ACGAGCGAAA CTGCTACAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTGC AGGCGCTCTA TGCCCTCCAG GCCCTTGTAG TGACCTTAGA CCAGCCCCCT 
AACCTGCTTG GGATGTTCTT CGATGCGCTG TATGACGAGG ACGTGGTGAA GGAGGACGCC 
TTCTATAGCT GGGAGAGTAG CAAGGACCCT GCTGAGCAGC AGGGCAAGGG CGTGGCCCTA 
AAATCTGTCA CGACTTTTTT CAAGTGGCTT CGTGAGGCGG AGGAGGAGGA GTCTGACCAC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_015462066.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317552.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

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CloneID	OBa45813
Clone ID Related Accession (Same CDS sequence)	XM_015462066.1 , XM_010801320.1
Accession Version	XM_015462066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC 
AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC 
TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT 
GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT 
CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG 
CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG 
GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA 
TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC 
CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC 
CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC 
CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG 
CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT 
GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC 
ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA 
AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG 
GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT 
CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA 
AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA 
GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG 
CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG 
AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG 
AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC 
CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC 
CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG 
CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT 
CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC 
CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG 
CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC 
AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA 
GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG 
ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT 
GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC 
GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG 
GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG 
GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317552.1
CDS	35..4858
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRILDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVPRADDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_015462066.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462066.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC 
AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC 
TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT 
GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT 
CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG 
CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG 
GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA 
TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC 
CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC 
CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC 
CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG 
CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT 
GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC 
ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA 
AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG 
GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT 
CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA 
AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA 
GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG 
CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG 
AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG 
AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC 
CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC 
CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG 
CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT 
CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC 
CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG 
CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC 
AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA 
GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG 
ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT 
GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC 
GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG 
GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG 
GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( XM_010801320.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799622.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

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CloneID	OBa45813
Clone ID Related Accession (Same CDS sequence)	XM_015462066.1 , XM_010801320.1
Accession Version	XM_010801320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC 
AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC 
TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT 
GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT 
CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG 
CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG 
GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA 
TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC 
CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC 
CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC 
CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG 
CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT 
GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC 
ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA 
AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG 
GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT 
CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA 
AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA 
GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG 
CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG 
AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG 
AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC 
CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC 
CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG 
CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT 
CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC 
CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG 
CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC 
AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA 
GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG 
ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT 
GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC 
GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG 
GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG 
GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799622.1
CDS	35..4858
Translation	MNKAPQPTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPSFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPPPVLMNQPPQIAPKRERKTIRILDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVVPRADDRSQGAIIGERPGLPGPEHSPSESQPSSPSPTPSPPPVLEPGSEPNLAVLSVPGDTMTTGMIQTSVEESTPTPPESGEPCCLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQVPSPLASHKVEILPEPNGTVPSENLEPEVESSPELAPLPPPACPSESPMPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEAIVSVTPPTVLSATPAVAPPVASPAQEEDMEEEEEEEEEGEAEGEKGGEEPLPQESTPVPAHLSQNSEVAVATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPGVPEVENQPPAGCNPSPEPEGSSVPLRSEEGEETWDSKEDKIQNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPSRLQGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVSMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRKSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPDGLRKAASLTEDRDRGREAVKREAALPPVSPPKAALSEEELEKKSRAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSAITREHMGRLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELITPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDVSAFVTAQKVEYTLGEELEAPGQRLLSFEELRRQLEKLLQEGSSNQRVFDWIEANLNEQQVTSNTLVRALMTTVCYSAIIFESSLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLDQPPNLLGMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTTFFKWLREAEEEESDHN

Target ORF information:

RefSeq Version	XM_010801320.1
Organism	Bos taurus(cattle)
Definition	Bos taurus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801320.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGCC CACAGGCCCC CCACCTGCCC CATCCCCTGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCACCG GTGGTGTTCA GTACGCCTCA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGCCG TTCCACATAT 
GTTGTCCCGA CACAGCAGTA TCCTGTGCAG CCGGGAGCCC CAAGCTTCTA CCCAGGTGCA 
AGCCCTACAG AGTTTGGGAC CTACGCTGGC GCTTACTACC CAGCCCAGGG TGTGCAGCAG 
TTTCCCACTG GTGTAGCTCC CCCACCTGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GGAAGACGAT CCGAATTCTA GACCCAAACC AAGGAGGGAA GGATATCACG 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CACCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAGGCTAA TGGGGAGACA CCCCAGGTTG CTGTCGTCCC TCGTGCAGAT 
GACCGGTCGC AGGGAGCAAT CATTGGGGAG CGGCCAGGGC TGCCTGGCCC AGAGCACAGC 
CCTTCAGAAT CCCAGCCTTC ATCACCTTCT CCAACCCCAT CACCACCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTGTTCCTG GGGACACAAT GACAACAGGG 
ATGATACAGA CGTCTGTAGA AGAATCAACC CCCACGCCCC CTGAAAGTGG GGAGCCGTGT 
TGCCTCTCTC CAGAACCCAC TCCCCTTGCT GAACCCATAT TGGAAGTAGA AGTGACGCTG 
AGCAAACCAG TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTCC CAGCCCCCTG 
GCATCTCACA AGGTGGAAAT TCTTCCTGAG CCTAATGGCA CAGTCCCATC TGAGAATTTG 
GAACCAGAGG TGGAGTCAAG CCCAGAGCTT GCCCCTCTCC CTCCTCCAGC TTGTCCCTCT 
GAATCCCCCA TGCCTATTGC TCCAACTGCC CAACCTGAGG AACTACTCAA CGGAGCCCCT 
TCACCACCAG CTGTGGACTT AAGCCCAGTC AGTGAACCAG AGGAACAGGC CAAGGAGGCT 
ATAGTGTCGG TGACTCCCCC CACTGTCCTT TCTGCTACCC CAGCTGTGGC TCCTCCAGTG 
GCTTCCCCTG CTCAGGAGGA GGACATGGAG GAGGAGGAAG AAGAGGAAGA GGAAGGAGAA 
GCTGAGGGTG AGAAGGGAGG AGAGGAACCT CTCCCCCAAG AGAGCACCCC TGTCCCAGCC 
CACCTATCCC AGAATTCGGA GGTGGCAGTA GCCACCCAAG TGGCAGTGTC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTCAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC 
TTCAAGGAGG CGAACCCAGG GGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCTGCAAT 
CCCAGCCCAG AGCCTGAGGG CAGCAGTGTG CCCCTGCGGT CTGAGGAAGG AGAGGAGACC 
TGGGACTCAA AGGAAGACAA GATTCAAAAT GCAGAGAATA TCCAGCCAGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AGGAGAAAAA GCGTTATGAC 
CGAGAGTTCC TGCTTGGCTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC TGAGGGATTG 
CCCCACATCA GTGATGTGGT GTTGGATAAG GCCAATAAAA CACCATTGCG ACCGCTGGAC 
CCCTCTAGAC TTCAGGGCAT AAATTGTGGC CCAGACTTCA CTCCGTCTTT TGCCAACCTT 
GGCCGACCAG CCCTTAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGAGGGCCGG CTGGCCTGGG ACCCCGGCGA TCTCAGCAGG GCCCCCGAAA GGAACCACGC 
AAGATCATTG CCACAGTGTC AATGACCGAA GATATAAAGC TGAACAAAGC AGAAAAGGCC 
TGGAAACCCA GTAGCAAGCG GACGGCGGCT GATAAGGACC GAGGAGAAGA GGATGCTGAT 
GGCAGCAAAA CCCAGGACCT GTTCCGCAGG GTGCGCTCCA TCTTGAATAA GCTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTG ACGCAGCTAG CCATTGATAC CGAGGAACGT 
CTCAAAGGGG TCATTGACCT CATCTTCGAG AAGGCCATTT CAGAACCCAA CTTCTCCGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAACTGTTG TTAAATCGAT GTCAGAAGGA GTTTGAAAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GGGGACGCCT GAAAGAAGAG CTGGAAGAGG CTCGAGACAT AGCCCGGCGG 
CGCTCGTTAG GGAATATCAA GTTTATCGGG GAGTTGTTCA AATTGAAGAT GTTAACAGAG 
GCAATCATGC ATGACTGTGT GGTTAAACTA CTTAAGAACC ATGATGAAGA GTCCCTTGAA 
TGCCTTTGCC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAGAA GGCCAAGCCC 
CGGATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AGGAAAAGAA GACTTCATCC 
CGGATCCGCT TTATGCTGCA GGATGTGCTG GATCTGCGAA AGAGCAACTG GGTGCCACGC 
CGTGGGGACC AGGGGCCCAA GACAATTGAC CAAATCCACA AGGAAGCTGA GATGGAGGAG 
CATCGGGAGC ACATAAAAGT GCAGCAGCTA ATGGCCAAGG GCAGCGACAA GCGTCGGGGT 
GGCCCTCCAG GCCCACCCAT CAGCCGTGGC CTTCCACTTG TGGATGATGG TGGCTGGAAC 
ACAGTACCCA TCAGCAAGGG CAGCCGCCCT ATTGACACTT CTCGACTCAC TAAGATCACA 
AAGCCTGGCT CCATTGATTC TAATAACCAG CTGTTTGCAC CTGGAGGCCG ATTGAGCTGG 
GGCAAGGGTA GCAGTGGAGG CTCAGGAGCC AAGCCCTCCG ATGCAGCATC AGAAGCTGCT 
CGTCCAGCTA CTAGTACCTT GAATCGCTTC TCAGCCCTTC AACAAGCAGT ACCAACAGAA 
AGCACAGACA ACAGACGAGT GGTGCAGAGG AGTAGCTTGA GCCGGGAACG AGGTGAGAAA 
GCTGGGGACC GGGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGTGACCG TGGTGACCGG 
CTTGATCGCG CCCGGACACC CGCCACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAACGG 
AGTAGAGAGC GGCCCTCTCA GCCTGATGGA CTACGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGCG GGCGGGAAGC TGTGAAACGA GAAGCTGCCC TCCCCCCGGT GAGTCCCCCG 
AAGGCTGCAC TCTCTGAAGA GGAGCTGGAG AAGAAATCCA GGGCCATCAT TGAGGAGTAC 
CTCCATCTCA ATGACATGAA GGAGGCAGTG CAGTGCGTGC AGGAGCTGGC TTCGCCCTCC 
CTGCTCTTTA TCTTTGTACG GCACGGCATC GAGTCCACGC TGGAGCGCAG CGCCATCACG 
CGGGAGCACA TGGGCCGGCT GCTGCACCAG CTGCTCTGTG CTGGGCACCT CTCCACTGCT 
CAGTACTACC AAGGGCTATA TGAAATTCTA GAGTTGGCTG AAGACATGGA AATTGACATC 
CCTCATGTGT GGCTCTACCT AGCAGAATTG ATAACGCCCA TTCTGCAGGA AGGTGGGGTG 
CCAATGGGGG AGCTGTTCAG GGAAATAACA AAACCTCTGA GACCCCTGGG CAAGGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGGCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAGGCTGG ACTCAGCTGG AAGGAATTTC TGCCTGAAGG CCAGGATGTC 
AGTGCCTTTG TCACTGCGCA GAAGGTGGAG TATACCCTGG GAGAGGAGTT AGAAGCCCCA 
GGCCAGAGGT TGCTGTCCTT TGAGGAGCTG CGCAGGCAGC TGGAGAAGCT GCTGCAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTTGACTGG ATAGAGGCCA ACCTGAACGA GCAGCAGGTG 
ACATCCAACA CATTAGTTCG AGCCCTTATG ACAACTGTCT GCTATTCTGC AATTATCTTT 
GAGTCTTCTC TCCGAGTGGA TGTTGCAGTG CTGAAAGCAC GAGCGAAACT GCTACAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTGCAG GCGCTCTATG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGACC AGCCCCCTAA CCTGCTTGGG ATGTTCTTCG ATGCGCTGTA TGACGAGGAC 
GTGGTGAAGG AGGACGCCTT CTATAGCTGG GAGAGTAGCA AGGACCCTGC TGAGCAGCAG 
GGCAAGGGCG TGGCCCTAAA ATCTGTCACG ACTTTTTTCA AGTGGCTTCG TGAGGCGGAG 
GAGGAGGAGT CTGACCACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EIF4G1
Entrez Gene ID	444858
Full Name	eukaryotic translation initiation factor 4 gamma 1
Synonyms	EIF4GI
General protein information	Preferred Names eukaryotic translation initiation factor 4 gamma 1 Names eukaryotic translation initiation factor 4 gamma 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	EIF4G1	XP_002802932.1
Canis lupus familiaris (dog)	EIF4G1	XP_005640005.1
Bos taurus (cattle)	EIF4G1	XP_003585711.2
Mus musculus (house mouse)	Eif4g1	NP_666053.2
Homo sapiens (human)	EIF4G1	NP_886553.3
Rattus norvegicus (Norway rat)	Eif4g1	XP_006248643.1
Gallus gallus (chicken)	EIF4G1	XP_004943468.1
Xenopus tropicalis (tropical clawed frog)	eif4g3	XP_004914456.1
Pan troglodytes (chimpanzee)	EIF4G1	XP_003310194.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EIF4G1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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