ADAM12 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADAM12
Entrez Gene ID	407229
Full Name	ADAM metallopeptidase domain 12
General protein information	Preferred Names ADAM metallopeptidase domain 12 Names disintegrin and metalloproteinase domain-containing protein 12 meltrin alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001156.1	NP_001001156.1	disintegrin and metalloproteinase domain-containing protein 12 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Adam12	XP_006230508.1
Bos taurus (cattle)	ADAM12	NP_001001156.1
Caenorhabditis elegans (roundworm)	adm-2	NP_510291.1
Homo sapiens (human)	ADAM12	NP_003465.3
Canis lupus familiaris (dog)	ADAM12	XP_005637899.1
Gallus gallus (chicken)	ADAM12	NP_001136322.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013468	XP_003436497.1
Danio rerio (zebrafish)	adam12	XP_691731.6
Xenopus tropicalis (tropical clawed frog)	adam12	NP_001035103.1
Pan troglodytes (chimpanzee)	ADAM12	XP_508106.2
	ADAM12	XP_001087980.2
Mus musculus (house mouse)	Adam12	NP_031426.2

Gene Ontology
Bibliography

Related articles in PubMed

A disintegrin and metalloprotease-12 is type I myofiber specific in Bos taurus and Bos indicus cattle.
Coles CA, Wadeson J, Knight MI, Cafe LM, Johns WH, White JD, Greenwood PL, McDonagh MB
Journal of animal science92(4)1473-83(2014 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Genomic organization and promoter analysis of the bovine ADAM12 gene.
Taniguchi Y, Doronbekov K, Yamada T, Sasaki Y, Takano A, Sugimoto Y
Animal biotechnology19(3)178-89(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest ADAM12 is upregulated in muscles with more slow-oxidative myofibres, such as the LM, and is linked to type I myofibers in cattle.
Title: A disintegrin and metalloprotease-12 is type I myofiber specific in Bos taurus and Bos indicus cattle.

The 5'- and 3'-regions of bovive ADAM12 have been analysed and compared to the human gene, and the promoter region has been cloned and sequenced.
Title: Genomic organization and promoter analysis of the bovine ADAM12 gene.

The following ADAM12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAM12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37073	NM_001001156.1 Latest version!	Bos taurus ADAM metallopeptidase domain 12 (ADAM12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADAM12 ( NM_001001156.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001001156.1 Bos taurus ADAM metallopeptidase domain 12 (ADAM12), mRNA.

Price & Availability↑

CloneID	OBa37073
Clone ID Related Accession (Same CDS sequence)	NM_001001156.1
Accession Version	NM_001001156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	disintegrin and metalloproteinase domain-containing protein 12 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB164682.1. ##Evidence-Data-START## Transcript exon combination :: AB164682.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCGC GCCCGCCGCC CGCGCCCACC GCCCGCGCCC TCCTGCTCGC CCTGGCCGGG 
GCTCTGCTGG CGCCGCGCGC GGCCCGAGGG GTGAACTTGG GAAGCCAGTT TGTGCCTTCC 
ATGCAGCAGG CCGGGACGAG GGATGGGGCA GGAGCCTATG AAGTGGTCAG TGTCTCTCTT 
TTGAATGGAG ATCCATGGAA CCCAATGAAG ACTGCAGACT CAGAGAATCA CCCAGGAGTG 
TTGAATTTTC GAATACAACT GACAAGCAAA GAGCTGATCG TGAACCTGGA AAGAAATGAA 
GGTCTCATCG CCAGCAGTTT CACGGAAACC CACTATCTAC AGGATGGCAC GGACATCTCT 
CTCATCCGAA ATTACACAGG TCACTGTTAT TATCATGGAC GTGTGCAAGG CTCTTTCGGA 
TCGGCGGTCA GTCTCAGCAC TTGTTCTGGT CTCAGGGGAC TCATTACATT TGAAAATAAC 
ACCTATATTT TGGAGCCAAT GAAAAATGCA ACCAACAGAT ACAAACTCTT CCCAGTGGAA 
AACTTGCCGG GCACCTGGGG ATCATGTGGA TCAAATACGT CGGGCCTCAC TCTGCACGAC 
AGGCTTCCAC TGGCCTCCCA GACCCAGACA AGAAGGCAAA AAAGAGAGAC CCTCAAGATG 
ACCAAGTACG TAGAGCTTGT CATCGTCGCC GATAACCGAG AGTTTCAGAG GCAAGGAAAA 
GATCTGGAAA AAGTCAAGCA GCGATTAATA GAGATCGCTA ATCACGTTGA CAAGTTTTAC 
CGACCACTGA ACATTCGAAT CGTGTTGGTG GGCGTGGAGG TGTGGAATGA CATTGACAAA 
TGCTCCATAA GTCAGGACCC ATTCACGAGC CTGCATGAGT TTCTAGACTG GAGAAAGATG 
AAGCTTCTGC CTCGCAAATC CCATGACAAC GCACAGCTCA TCAGTGGAGT TTATTTCCAA 
GGAACCACCG TCGGCATGGC GCCCATCATG AGTATGTGCA CAGCAGAGCA GTCCGGGGGC 
ATCGTCATGG ACCATTCAGA CAGCCCCCTG GGTGCGGCCG TGACCCTGGC ACACGAGCTG 
GGCCACAATT TTGGGATGAA CCATGACACC CTGGAGAGAG GCTGCAGCTG CAAGACGGCC 
GCCGACAAAG GAGGCTGCAT CATGAACCCT TCAACCGGGT TCCCGTTCCC CATGATGTTC 
AGCAGCTGCA GCAGGAAGGA CCTGGAGGCC AGCCTGCAGA AGGGCATGGG CATGTGTCTG 
TTCAACCTGC CGGAAGTGAA GCAGTCCTTC GGGGGCCAGA AGTGCGGGAA CGGATATGTT 
GAAGAAGGAG AGGAATGTGA CTGTGGGGAA CCAGAGGAAT GTCAGAATCT CTGCTGCAAC 
GCCACCACCT GTGCCCTGAA GCCAGACGCT GTCTGCGCGC ACGGACTGTG CTGTGAAAAC 
TGCCGGCTGA AGCCGGCGGG AACCGCGTGT AGGGACCCCA GCAACGCCTG CGACCTCCCG 
GAGTTCTGCA CTGGGGCCAG TCCTCACTGC CCAGCCAATG TGTACCTGCA CGATGGGCAC 
CCATGCCAGG GGGTGGATGG CTACTGCTAC AATGGCATCT GCCAGACCCA TGAGCAGCAG 
TGCGTCACCC TCTGGGGACC AGGTGCTAAA CCTGCCCCTG GGATCTGCTT TGAGAGAGTC 
AACTCCGCAG GTGACCCTTA TGGCAACTGC GGCAAAGATT CCAAGAGCTC CTTTGCCAAG 
TGCGCGATGA GAGACGCCAA GTGTGGAAAA ATCCAGTGTC AAGGAGGTGC CAGCCGACCA 
GTCATTGGTA CCAATGCCGT TTCCATAGAA ACGAATATCC CCCTGCAGGA AGGAGGCCGG 
ATTCTGTGCC GTGGAACCCA CGTGTACTTG GGTGATGACT TGCCGGACCC GGGGCTCGTG 
CTTGCCGGCA CAAAGTGCGC AGATGGAAAA ATCTGCCTGA ATCGTCGGTG TCAGAACGTC 
AGTGTCTTTG GTGTGCATGA GTGTGCAGTG CAGTGCCACG GCAGGGGGGT GTGCAACAAC 
AGGAAGAACT GCCATTGCGA GCCCCACTGG GCACCTCCCT TCTGTGACAG GTTTGGCTTC 
GGAGGGAGCA CGGACAGCGG CCCCGTCCGG CAAGCAGATA ACCAGGGTTT AACGATAGGA 
ATTCTGGTCA CCATCCTCTG TCTTCTTGCT GCTGGATGTG TGGTTTACCT CAAAAGGAAG 
ACGTTGATAC GACTGCTGTT TACAGATAAA AAAAACACCA TCGAAAAGCT AAGGTGCGTG 
CGCCCTCCCC GGCCCCCCAG GGGCTCCCAG CCCGGCCAGG CCCACCTCAC CCACCTCAGC 
AAAGGCCCGA CGAGGAAGCC GCCACCTTCC AGCACACCTA AGGACAACAC CCGGAGAGTG 
CCGCCATGTC AGCATGTGGA CATCAGCAGA CCCCTCAAAG CCCCCGACGC CCCTTGGCCG 
AGTTCCCCGC AGCGAGGGCT CCCACCCCTG CACCAGGCTC CTCGTGTGCC CTGCGTCCCC 
GCCAGACCCC TGCCGGCCAA CCCTGCACTC AGGCAGGCCC AGGGAACTCG TAAGCCAAGC 
CCCCCTCAGA AGCCGCTGCC TGCAGACCCT TTGAGCAAGA CGGCTCGCCT GGCCGGCGCC 
CCAGCCAGGT CCCCGGGACG GCAGGAGTCT GCGCTCCGCC TGGCGCCCAT CAGACCTGCT 
CCGAAACAGC CCCATCCCGT GCCCAGACCC GCCCCCATTG CCTATGCTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001156.1
CDS	298..3051
Translation	MAARPPPAPTARALLLALAGALLAPRAARGVNLGSQFVPSMQQAGTRDGAGAYEVVSVSLLNGDPWNPMKTADSENHPGVLNFRIQLTSKELIVNLERNEGLIASSFTETHYLQDGTDISLIRNYTGHCYYHGRVQGSFGSAVSLSTCSGLRGLITFENNTYILEPMKNATNRYKLFPVENLPGTWGSCGSNTSGLTLHDRLPLASQTQTRRQKRETLKMTKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDIDKCSISQDPFTSLHEFLDWRKMKLLPRKSHDNAQLISGVYFQGTTVGMAPIMSMCTAEQSGGIVMDHSDSPLGAAVTLAHELGHNFGMNHDTLERGCSCKTAADKGGCIMNPSTGFPFPMMFSSCSRKDLEASLQKGMGMCLFNLPEVKQSFGGQKCGNGYVEEGEECDCGEPEECQNLCCNATTCALKPDAVCAHGLCCENCRLKPAGTACRDPSNACDLPEFCTGASPHCPANVYLHDGHPCQGVDGYCYNGICQTHEQQCVTLWGPGAKPAPGICFERVNSAGDPYGNCGKDSKSSFAKCAMRDAKCGKIQCQGGASRPVIGTNAVSIETNIPLQEGGRILCRGTHVYLGDDLPDPGLVLAGTKCADGKICLNRRCQNVSVFGVHECAVQCHGRGVCNNRKNCHCEPHWAPPFCDRFGFGGSTDSGPVRQADNQGLTIGILVTILCLLAAGCVVYLKRKTLIRLLFTDKKNTIEKLRCVRPPRPPRGSQPGQAHLTHLSKGPTRKPPPSSTPKDNTRRVPPCQHVDISRPLKAPDAPWPSSPQRGLPPLHQAPRVPCVPARPLPANPALRQAQGTRKPSPPQKPLPADPLSKTARLAGAPARSPGRQESALRLAPIRPAPKQPHPVPRPAPIAYAK

Target ORF information:

RefSeq Version	NM_001001156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ADAM metallopeptidase domain 12 (ADAM12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001156.1

ORF Insert Sequence:

ATGGCCGCGC GCCCGCCGCC CGCGCCCACC GCCCGCGCCC TCCTGCTCGC CCTGGCCGGG 
GCTCTGCTGG CGCCGCGCGC GGCCCGAGGG GTGAACTTGG GAAGCCAGTT TGTGCCTTCC 
ATGCAGCAGG CCGGGACGAG GGATGGGGCA GGAGCCTATG AAGTGGTCAG TGTCTCTCTT 
TTGAATGGAG ATCCATGGAA CCCAATGAAG ACTGCAGACT CAGAGAATCA CCCAGGAGTG 
TTGAATTTTC GAATACAACT GACAAGCAAA GAGCTGATCG TGAACCTGGA AAGAAATGAA 
GGTCTCATCG CCAGCAGTTT CACGGAAACC CACTATCTAC AGGATGGCAC GGACATCTCT 
CTCATCCGAA ATTACACAGG TCACTGTTAT TATCATGGAC GTGTGCAAGG CTCTTTCGGA 
TCGGCGGTCA GTCTCAGCAC TTGTTCTGGT CTCAGGGGAC TCATTACATT TGAAAATAAC 
ACCTATATTT TGGAGCCAAT GAAAAATGCA ACCAACAGAT ACAAACTCTT CCCAGTGGAA 
AACTTGCCGG GCACCTGGGG ATCATGTGGA TCAAATACGT CGGGCCTCAC TCTGCACGAC 
AGGCTTCCAC TGGCCTCCCA GACCCAGACA AGAAGGCAAA AAAGAGAGAC CCTCAAGATG 
ACCAAGTACG TAGAGCTTGT CATCGTCGCC GATAACCGAG AGTTTCAGAG GCAAGGAAAA 
GATCTGGAAA AAGTCAAGCA GCGATTAATA GAGATCGCTA ATCACGTTGA CAAGTTTTAC 
CGACCACTGA ACATTCGAAT CGTGTTGGTG GGCGTGGAGG TGTGGAATGA CATTGACAAA 
TGCTCCATAA GTCAGGACCC ATTCACGAGC CTGCATGAGT TTCTAGACTG GAGAAAGATG 
AAGCTTCTGC CTCGCAAATC CCATGACAAC GCACAGCTCA TCAGTGGAGT TTATTTCCAA 
GGAACCACCG TCGGCATGGC GCCCATCATG AGTATGTGCA CAGCAGAGCA GTCCGGGGGC 
ATCGTCATGG ACCATTCAGA CAGCCCCCTG GGTGCGGCCG TGACCCTGGC ACACGAGCTG 
GGCCACAATT TTGGGATGAA CCATGACACC CTGGAGAGAG GCTGCAGCTG CAAGACGGCC 
GCCGACAAAG GAGGCTGCAT CATGAACCCT TCAACCGGGT TCCCGTTCCC CATGATGTTC 
AGCAGCTGCA GCAGGAAGGA CCTGGAGGCC AGCCTGCAGA AGGGCATGGG CATGTGTCTG 
TTCAACCTGC CGGAAGTGAA GCAGTCCTTC GGGGGCCAGA AGTGCGGGAA CGGATATGTT 
GAAGAAGGAG AGGAATGTGA CTGTGGGGAA CCAGAGGAAT GTCAGAATCT CTGCTGCAAC 
GCCACCACCT GTGCCCTGAA GCCAGACGCT GTCTGCGCGC ACGGACTGTG CTGTGAAAAC 
TGCCGGCTGA AGCCGGCGGG AACCGCGTGT AGGGACCCCA GCAACGCCTG CGACCTCCCG 
GAGTTCTGCA CTGGGGCCAG TCCTCACTGC CCAGCCAATG TGTACCTGCA CGATGGGCAC 
CCATGCCAGG GGGTGGATGG CTACTGCTAC AATGGCATCT GCCAGACCCA TGAGCAGCAG 
TGCGTCACCC TCTGGGGACC AGGTGCTAAA CCTGCCCCTG GGATCTGCTT TGAGAGAGTC 
AACTCCGCAG GTGACCCTTA TGGCAACTGC GGCAAAGATT CCAAGAGCTC CTTTGCCAAG 
TGCGCGATGA GAGACGCCAA GTGTGGAAAA ATCCAGTGTC AAGGAGGTGC CAGCCGACCA 
GTCATTGGTA CCAATGCCGT TTCCATAGAA ACGAATATCC CCCTGCAGGA AGGAGGCCGG 
ATTCTGTGCC GTGGAACCCA CGTGTACTTG GGTGATGACT TGCCGGACCC GGGGCTCGTG 
CTTGCCGGCA CAAAGTGCGC AGATGGAAAA ATCTGCCTGA ATCGTCGGTG TCAGAACGTC 
AGTGTCTTTG GTGTGCATGA GTGTGCAGTG CAGTGCCACG GCAGGGGGGT GTGCAACAAC 
AGGAAGAACT GCCATTGCGA GCCCCACTGG GCACCTCCCT TCTGTGACAG GTTTGGCTTC 
GGAGGGAGCA CGGACAGCGG CCCCGTCCGG CAAGCAGATA ACCAGGGTTT AACGATAGGA 
ATTCTGGTCA CCATCCTCTG TCTTCTTGCT GCTGGATGTG TGGTTTACCT CAAAAGGAAG 
ACGTTGATAC GACTGCTGTT TACAGATAAA AAAAACACCA TCGAAAAGCT AAGGTGCGTG 
CGCCCTCCCC GGCCCCCCAG GGGCTCCCAG CCCGGCCAGG CCCACCTCAC CCACCTCAGC 
AAAGGCCCGA CGAGGAAGCC GCCACCTTCC AGCACACCTA AGGACAACAC CCGGAGAGTG 
CCGCCATGTC AGCATGTGGA CATCAGCAGA CCCCTCAAAG CCCCCGACGC CCCTTGGCCG 
AGTTCCCCGC AGCGAGGGCT CCCACCCCTG CACCAGGCTC CTCGTGTGCC CTGCGTCCCC 
GCCAGACCCC TGCCGGCCAA CCCTGCACTC AGGCAGGCCC AGGGAACTCG TAAGCCAAGC 
CCCCCTCAGA AGCCGCTGCC TGCAGACCCT TTGAGCAAGA CGGCTCGCCT GGCCGGCGCC 
CCAGCCAGGT CCCCGGGACG GCAGGAGTCT GCGCTCCGCC TGGCGCCCAT CAGACCTGCT 
CCGAAACAGC CCCATCCCGT GCCCAGACCC GCCCCCATTG CCTATGCTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A disintegrin and metalloprotease-12 is type I myofiber specific in Bos taurus and Bos indicus cattle.
Journal of animal science92(4)1473-83(2014 Apr)
Coles CA,Wadeson J,Knight MI,Cafe LM,Johns WH,White JD,Greenwood PL,McDonagh MB

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Genomic organization and promoter analysis of the bovine ADAM12 gene.
Animal biotechnology19(3)178-89(2008)
Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

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ADAM12 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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