gRNAs
The following COL2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa134046 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001113224.2 | |
| Accession Version | NM_001113224.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-13 | |
| Organism | Bos taurus(cattle) | |
| Product | collagen alpha-1(II) chain isoform 2 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02012985.1 and DAAA02012986.1. On Oct 1, 2015 this sequence version replaced NM_001113224.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein. The resulting protein (isoform 2) lacks the von Willebrand factor type C (VWC) domain, compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_033150.2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145430 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001106695.1 |
| CDS | 27..4283 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001113224.2 |
1 | ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa134048 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001001135.3 | |
| Accession Version | NM_001001135.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-13 | |
| Organism | Bos taurus(cattle) | |
| Product | collagen alpha-1(II) chain isoform 1 preproprotein | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02012985.1 and DAAA02012986.1. On Oct 1, 2015 this sequence version replaced NM_001001135.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_001844.4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145425 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001001135.2 |
| CDS | 27..4490 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001001135.3 |
1 | ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Lethal chondrodysplasia in a family of Holstein cattle is associated with a de novo splice site variant of COL2A1. |
Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system. |
Two subsystems of meniscal collagen and their different thermal stabilities. |
Disc strain and resulting positive mRNA expression from application of a noninvasive treatment. |
Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi. |
