TJP2 cDNA ORF clone, Bos taurus(cattle)

The following TJP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236941 XM_005210007.4
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa236942 XM_005210008.2
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa236943 XM_010807869.3
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236943 XM_024995498.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236944 XM_024995494.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236945 XM_024995495.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236946 XM_024995496.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236947 XM_024995497.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa236948 XM_024995499.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa39431 NM_001102482.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21973 XM_005210007.3
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa21974 XM_005210008.1
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa21975 XM_010807869.2
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa45681 XM_005210009.3
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa45682 XM_010807868.2
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa45683 XM_010807870.2
Latest version!
Bos taurus tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236941
    Clone ID Related Accession (Same CDS sequence) XM_005210007.4
    Accession Version XM_005210007.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210007.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCACA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TCTGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCAGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGAGCATA AGGAAACCCA GCCCAGAGCC ACGAGCTCAG
    ATGAGGAGGG CTGCTAGCAG AGATCAACTT AGGGACAATA GCCCGCCCCC GACATTCAAG
    CCAGAGCCGC CCAAGGCCAA AACCCAAAAC AGAGAAGAAT CTTTTGACTT CTCCAGATCT
    TATGAATACA AGTCAAACCC CTCAGCCGCT GCTGGTAACG AAATTCCAGG GGCATCTACC
    AAAGGTTGTC CTCCTCCTAT TGCAGCGAAA CCCACTTTTG GACGGCCTGT ACTGAAGCCC
    TCCACTCCCG TCCCTCCCCC AGAGAGTGAG GAGACGGGAG AGGGCAGTGA GGAGCAGCAC
    AATGCTCCCA AATCTGTCCT GGGCAAAGTA AAAATATTTG AGAAGATGGA TCACAAGGCA
    AAGTTACAGA GAATGCAAGA GCTCCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ATATCTATGC AGTTCCAATC AAAACACACA AGCCGGACCC TGGCCCACCC
    CAGTACACAA GTTCCAGGCC CCCCGAGCCA CAGAAAGGCC CTGCCAGACT TTATCAGGAT
    CCCAGAGGAA GCTATGGCAG TGATGCTGAG GAGGAGGAAT ACCGGCAGCA GCTGTCAGAA
    CACTCAAGGC ACGGTTATTA CGGCCAGCCT TCCCGATACC GGGACACGGA ATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210064.2
    CDS22..3558
    Translation

    Target ORF information:

    RefSeq Version XM_005210007.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210007.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCACA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TCTGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCAGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGAGCATA AGGAAACCCA GCCCAGAGCC ACGAGCTCAG
    ATGAGGAGGG CTGCTAGCAG AGATCAACTT AGGGACAATA GCCCGCCCCC GACATTCAAG
    CCAGAGCCGC CCAAGGCCAA AACCCAAAAC AGAGAAGAAT CTTTTGACTT CTCCAGATCT
    TATGAATACA AGTCAAACCC CTCAGCCGCT GCTGGTAACG AAATTCCAGG GGCATCTACC
    AAAGGTTGTC CTCCTCCTAT TGCAGCGAAA CCCACTTTTG GACGGCCTGT ACTGAAGCCC
    TCCACTCCCG TCCCTCCCCC AGAGAGTGAG GAGACGGGAG AGGGCAGTGA GGAGCAGCAC
    AATGCTCCCA AATCTGTCCT GGGCAAAGTA AAAATATTTG AGAAGATGGA TCACAAGGCA
    AAGTTACAGA GAATGCAAGA GCTCCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ATATCTATGC AGTTCCAATC AAAACACACA AGCCGGACCC TGGCCCACCC
    CAGTACACAA GTTCCAGGCC CCCCGAGCCA CAGAAAGGCC CTGCCAGACT TTATCAGGAT
    CCCAGAGGAA GCTATGGCAG TGATGCTGAG GAGGAGGAAT ACCGGCAGCA GCTGTCAGAA
    CACTCAAGGC ACGGTTATTA CGGCCAGCCT TCCCGATACC GGGACACGGA ATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236942
    Clone ID Related Accession (Same CDS sequence) XM_005210008.2
    Accession Version XM_005210008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGC GAGGAGATCG CGGGTTTCCA TCTCGGCGGG AAATGTCAGG TTGGCTCCCT 
    GCTCCAGGCA TGGAAGAACT GATATGGGAA CAGTACACTG TGACCCTACA GAAGGATTCC
    AAGAGAGGAT TTGGAATTGC AGTATCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACATCCA TCGTCATTTC TGATGTGCTC CCGGGTGGGC CCGCCGATGG GCTGCTTCAA
    GAGAATGACA GGGTGGTCAT GGTTAATGGC ACCCCTATGG AAGACGTGCT CCATTCATTT
    GCGGTTCAGC AGCTGAGGAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCT GCAGGACAGT CCTCCTGTCC ACGAAGATGA CCGGGCTTTC
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGC GCCCGCAGCG GCTACAGCGA GAGGAGCCGG
    CCCAGCAGCC GCGGAGGGCG CAGCCGCAGC TGGGAGGAGA GCCCAGAGAG GGGCCGTCCC
    CATGACCGGG TGCGCAGCCG GGATCGAGGC CGGAGTCTGG AGCGGGGCCT GGACCACGAC
    GACGACTACG GGCGAGCCCG GGAGCACAGC CGTGGCCGCA GCCTCGAGCG GGGCCTAGAC
    CAAGATGATT ACAGGCGAGC CCGGGAGAGC AGCCGCGGCC GAAGCATTGA CCCGGTCTAC
    GATCGCGCCT ACACCCCTGA GGGGGAGTAC GGCCACAGGG CCCAGCCTGA TGCCCGGTAT
    GGGGCATCCC AGAGCCGCAG CCGTGAGCAC CTCCACTCCC GCAGCCCCAG CCCGGAGCCC
    CGGGGGCGTC CGGACTCGGA CAAGCCTATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAATATG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTAGCAACTA AAGATGGCAA CCTGCATGAA GGAGACATAA TTCTCAAGAT AAACGGAACC
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAACTGA TAGAAAAGTC AAGAGGGAAA
    CTCCAGCTCG TGGTGCTGAG GGACAGCAAA CAGACACTTA TCAACATCCC CTCATTAAAT
    GACAGCGACT CAGAAATAGA AGATATCTCA GAAATAGAGT CCAACCGATC GTTTTCTCCA
    GAGGAGAGGC AGCAGCAGTA TTCTGATTAT GATTACCATT CCTCAAATGA AAAGCTAAAG
    GAGAGACCAA ATTCAAGAGA GGACATGCAG AGCAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCACTGG GGATATGGCG GCTGCGGGCA GCGTGGAGCC CAGCAAGGAA
    CCCAAATACC AAGAGGAAGC CCCAGTTCCT CCACCAAAAC CAGCCCCGAG AGCTGTTCTT
    CGTCCAAGTC CTGAAGATTT AGCAATATAT GGCCCCAATA CCAAAATGGT GAGGTTCAAG
    AAGGGAGACA GTGTGGGCCT CCGGCTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCC
    GGCATTCAAG AGGGTACCTC CGCGGAGCAA GAAGGTCTTC AAGAAGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGACTC GTTCGGGAGG ATGCTGTTCT CTACCTGTTA
    GAAATCCCCA AAGGTGAAAC GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATACTGG CTTGTGGCAG AGGGGATTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGTGT GGTAGATACA
    CTCTACGATG GCAAACTGGG CCACTGGCTG GCCGTGAGGA TCGGGAACGA ACTGGAGAAA
    GGCTTAATCC CCAACAAAAG CAGAGCGGAA CAAATGGCCA GCGTTCAGAA TGCCCAGAGA
    GACACCACCG GGGACAGGGC AGACTTCTGG AGGATGCGCG GCCAGAGATC AGGTGTGAAG
    AAGAACCTAC GGAAGAGTCG GGAGGACCTG ACTGCCGCCG TGACAGTCAG CACCAAGTTC
    CCGGCCTACG AGAGGGTTTT GCTTCGAGAA GCTGGTTTCA AGAGACCTGT TGTCTTATTT
    GGCCCCATAG CAGATATAGC CCTGGAGAAG TTGGCAAATG ACTTGCCTGA CCTGTTCCAA
    ACTGCTAAAA CAGAACCAAG AGACGCAGGA TCTGAGAAAT CCACCGGGGT GGTGCGGTTA
    AATACCGTGA GACAAATTAT TGAACAGGAT AAGCATGCAC TCCTGGATGT AACTCCTAAG
    GCTGTGGACC TGTTGAACTA TACTCAGTGG TTCCCGATTG TGATCTTTTT CAACCCAGAC
    TCTAGGCAAG GTGTCAAAAC TATGAGACAG AGGTTGAATC CCTCGTCCAA CAAAAGTTCT
    CGGAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACTT GTGCACATCT TTTCACAGCT
    ACAATCAACC TCAATTCAGC CAACGACAGC TGGTTTGGCA GCTTGAAAGA CACGATTCAG
    CATCAGCAAG GAGAAGCAGT CTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAT
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCGCTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAATAGC CCGCCCCCGA CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAAAACAG AGAAGAATCT TTTGACTTCT CCAGATCTTA TGAATACAAG
    TCAAACCCCT CAGCCGCTGC TGGTAACGAA ATTCCAGGGG CATCTACCAA AGGTTGTCCT
    CCTCCTATTG CAGCGAAACC CACTTTTGGA CGGCCTGTAC TGAAGCCCTC CACTCCCGTC
    CCTCCCCCAG AGAGTGAGGA GACGGGAGAG GGCAGTGAGG AGCAGCACAA TGCTCCCAAA
    TCTGTCCTGG GCAAAGTAAA AATATTTGAG AAGATGGATC ACAAGGCAAA GTTACAGAGA
    ATGCAAGAGC TCCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACACACAAG CCGGACCCTG GCCCACCCCA GTACACAAGT
    TCCAGGCCCC CCGAGCCACA GAAAGGCCCT GCCAGACTTT ATCAGGATCC CAGAGGAAGC
    TATGGCAGTG ATGCTGAGGA GGAGGAATAC CGGCAGCAGC TGTCAGAACA CTCAAGGCAC
    GGTTATTACG GCCAGCCTTC CCGATACCGG GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210065.2
    CDS136..3660
    Translation

    Target ORF information:

    RefSeq Version XM_005210008.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGC GAGGAGATCG CGGGTTTCCA TCTCGGCGGG AAATGTCAGG TTGGCTCCCT 
    GCTCCAGGCA TGGAAGAACT GATATGGGAA CAGTACACTG TGACCCTACA GAAGGATTCC
    AAGAGAGGAT TTGGAATTGC AGTATCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACATCCA TCGTCATTTC TGATGTGCTC CCGGGTGGGC CCGCCGATGG GCTGCTTCAA
    GAGAATGACA GGGTGGTCAT GGTTAATGGC ACCCCTATGG AAGACGTGCT CCATTCATTT
    GCGGTTCAGC AGCTGAGGAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCT GCAGGACAGT CCTCCTGTCC ACGAAGATGA CCGGGCTTTC
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGC GCCCGCAGCG GCTACAGCGA GAGGAGCCGG
    CCCAGCAGCC GCGGAGGGCG CAGCCGCAGC TGGGAGGAGA GCCCAGAGAG GGGCCGTCCC
    CATGACCGGG TGCGCAGCCG GGATCGAGGC CGGAGTCTGG AGCGGGGCCT GGACCACGAC
    GACGACTACG GGCGAGCCCG GGAGCACAGC CGTGGCCGCA GCCTCGAGCG GGGCCTAGAC
    CAAGATGATT ACAGGCGAGC CCGGGAGAGC AGCCGCGGCC GAAGCATTGA CCCGGTCTAC
    GATCGCGCCT ACACCCCTGA GGGGGAGTAC GGCCACAGGG CCCAGCCTGA TGCCCGGTAT
    GGGGCATCCC AGAGCCGCAG CCGTGAGCAC CTCCACTCCC GCAGCCCCAG CCCGGAGCCC
    CGGGGGCGTC CGGACTCGGA CAAGCCTATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAATATG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTAGCAACTA AAGATGGCAA CCTGCATGAA GGAGACATAA TTCTCAAGAT AAACGGAACC
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAACTGA TAGAAAAGTC AAGAGGGAAA
    CTCCAGCTCG TGGTGCTGAG GGACAGCAAA CAGACACTTA TCAACATCCC CTCATTAAAT
    GACAGCGACT CAGAAATAGA AGATATCTCA GAAATAGAGT CCAACCGATC GTTTTCTCCA
    GAGGAGAGGC AGCAGCAGTA TTCTGATTAT GATTACCATT CCTCAAATGA AAAGCTAAAG
    GAGAGACCAA ATTCAAGAGA GGACATGCAG AGCAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCACTGG GGATATGGCG GCTGCGGGCA GCGTGGAGCC CAGCAAGGAA
    CCCAAATACC AAGAGGAAGC CCCAGTTCCT CCACCAAAAC CAGCCCCGAG AGCTGTTCTT
    CGTCCAAGTC CTGAAGATTT AGCAATATAT GGCCCCAATA CCAAAATGGT GAGGTTCAAG
    AAGGGAGACA GTGTGGGCCT CCGGCTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCC
    GGCATTCAAG AGGGTACCTC CGCGGAGCAA GAAGGTCTTC AAGAAGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGACTC GTTCGGGAGG ATGCTGTTCT CTACCTGTTA
    GAAATCCCCA AAGGTGAAAC GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATACTGG CTTGTGGCAG AGGGGATTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGTGT GGTAGATACA
    CTCTACGATG GCAAACTGGG CCACTGGCTG GCCGTGAGGA TCGGGAACGA ACTGGAGAAA
    GGCTTAATCC CCAACAAAAG CAGAGCGGAA CAAATGGCCA GCGTTCAGAA TGCCCAGAGA
    GACACCACCG GGGACAGGGC AGACTTCTGG AGGATGCGCG GCCAGAGATC AGGTGTGAAG
    AAGAACCTAC GGAAGAGTCG GGAGGACCTG ACTGCCGCCG TGACAGTCAG CACCAAGTTC
    CCGGCCTACG AGAGGGTTTT GCTTCGAGAA GCTGGTTTCA AGAGACCTGT TGTCTTATTT
    GGCCCCATAG CAGATATAGC CCTGGAGAAG TTGGCAAATG ACTTGCCTGA CCTGTTCCAA
    ACTGCTAAAA CAGAACCAAG AGACGCAGGA TCTGAGAAAT CCACCGGGGT GGTGCGGTTA
    AATACCGTGA GACAAATTAT TGAACAGGAT AAGCATGCAC TCCTGGATGT AACTCCTAAG
    GCTGTGGACC TGTTGAACTA TACTCAGTGG TTCCCGATTG TGATCTTTTT CAACCCAGAC
    TCTAGGCAAG GTGTCAAAAC TATGAGACAG AGGTTGAATC CCTCGTCCAA CAAAAGTTCT
    CGGAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACTT GTGCACATCT TTTCACAGCT
    ACAATCAACC TCAATTCAGC CAACGACAGC TGGTTTGGCA GCTTGAAAGA CACGATTCAG
    CATCAGCAAG GAGAAGCAGT CTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAT
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCGCTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAATAGC CCGCCCCCGA CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAAAACAG AGAAGAATCT TTTGACTTCT CCAGATCTTA TGAATACAAG
    TCAAACCCCT CAGCCGCTGC TGGTAACGAA ATTCCAGGGG CATCTACCAA AGGTTGTCCT
    CCTCCTATTG CAGCGAAACC CACTTTTGGA CGGCCTGTAC TGAAGCCCTC CACTCCCGTC
    CCTCCCCCAG AGAGTGAGGA GACGGGAGAG GGCAGTGAGG AGCAGCACAA TGCTCCCAAA
    TCTGTCCTGG GCAAAGTAAA AATATTTGAG AAGATGGATC ACAAGGCAAA GTTACAGAGA
    ATGCAAGAGC TCCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACACACAAG CCGGACCCTG GCCCACCCCA GTACACAAGT
    TCCAGGCCCC CCGAGCCACA GAAAGGCCCT GCCAGACTTT ATCAGGATCC CAGAGGAAGC
    TATGGCAGTG ATGCTGAGGA GGAGGAATAC CGGCAGCAGC TGTCAGAACA CTCAAGGCAC
    GGTTATTACG GCCAGCCTTC CCGATACCGG GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236943
    Clone ID Related Accession (Same CDS sequence) XM_010807869.3 , XM_024995498.1
    Accession Version XM_010807869.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGAGCATAA GGAAACCCAG CCCAGAGCCA CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA
    GATCAACTTA GGGACAATAG CCCGCCCCCG ACATTCAAGC CAGAGCCGCC CAAGGCCAAA
    ACCCAAAACA GAGAAGAATC TTTTGACTTC TCCAGATCTT ATGAATACAA GTCAAACCCC
    TCAGCCGCTG CTGGTAACGA AATTCCAGGG GCATCTACCA AAGGTTGTCC TCCTCCTATT
    GCAGCGAAAC CCACTTTTGG ACGGCCTGTA CTGAAGCCCT CCACTCCCGT CCCTCCCCCA
    GAGAGTGAGG AGACGGGAGA GGGCAGTGAG GAGCAGCACA ATGCTCCCAA ATCTGTCCTG
    GGCAAAGTAA AAATATTTGA GAAGATGGAT CACAAGGCAA AGTTACAGAG AATGCAAGAG
    CTCCAAGAAG CACAGAATGC AAGGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806171.2
    CDS567..4022
    Translation

    Target ORF information:

    RefSeq Version XM_010807869.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807869.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGAGCATAA GGAAACCCAG CCCAGAGCCA CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA
    GATCAACTTA GGGACAATAG CCCGCCCCCG ACATTCAAGC CAGAGCCGCC CAAGGCCAAA
    ACCCAAAACA GAGAAGAATC TTTTGACTTC TCCAGATCTT ATGAATACAA GTCAAACCCC
    TCAGCCGCTG CTGGTAACGA AATTCCAGGG GCATCTACCA AAGGTTGTCC TCCTCCTATT
    GCAGCGAAAC CCACTTTTGG ACGGCCTGTA CTGAAGCCCT CCACTCCCGT CCCTCCCCCA
    GAGAGTGAGG AGACGGGAGA GGGCAGTGAG GAGCAGCACA ATGCTCCCAA ATCTGTCCTG
    GGCAAAGTAA AAATATTTGA GAAGATGGAT CACAAGGCAA AGTTACAGAG AATGCAAGAG
    CTCCAAGAAG CACAGAATGC AAGGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236943
    Clone ID Related Accession (Same CDS sequence) XM_010807869.3 , XM_024995498.1
    Accession Version XM_024995498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGAGCATAA GGAAACCCAG CCCAGAGCCA CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA
    GATCAACTTA GGGACAATAG CCCGCCCCCG ACATTCAAGC CAGAGCCGCC CAAGGCCAAA
    ACCCAAAACA GAGAAGAATC TTTTGACTTC TCCAGATCTT ATGAATACAA GTCAAACCCC
    TCAGCCGCTG CTGGTAACGA AATTCCAGGG GCATCTACCA AAGGTTGTCC TCCTCCTATT
    GCAGCGAAAC CCACTTTTGG ACGGCCTGTA CTGAAGCCCT CCACTCCCGT CCCTCCCCCA
    GAGAGTGAGG AGACGGGAGA GGGCAGTGAG GAGCAGCACA ATGCTCCCAA ATCTGTCCTG
    GGCAAAGTAA AAATATTTGA GAAGATGGAT CACAAGGCAA AGTTACAGAG AATGCAAGAG
    CTCCAAGAAG CACAGAATGC AAGGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851266.1
    CDS202..3657
    Translation

    Target ORF information:

    RefSeq Version XM_024995498.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGAGCATAA GGAAACCCAG CCCAGAGCCA CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA
    GATCAACTTA GGGACAATAG CCCGCCCCCG ACATTCAAGC CAGAGCCGCC CAAGGCCAAA
    ACCCAAAACA GAGAAGAATC TTTTGACTTC TCCAGATCTT ATGAATACAA GTCAAACCCC
    TCAGCCGCTG CTGGTAACGA AATTCCAGGG GCATCTACCA AAGGTTGTCC TCCTCCTATT
    GCAGCGAAAC CCACTTTTGG ACGGCCTGTA CTGAAGCCCT CCACTCCCGT CCCTCCCCCA
    GAGAGTGAGG AGACGGGAGA GGGCAGTGAG GAGCAGCACA ATGCTCCCAA ATCTGTCCTG
    GGCAAAGTAA AAATATTTGA GAAGATGGAT CACAAGGCAA AGTTACAGAG AATGCAAGAG
    CTCCAAGAAG CACAGAATGC AAGGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236944
    Clone ID Related Accession (Same CDS sequence) XM_024995494.1
    Accession Version XM_024995494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCACA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TCTGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCAGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGGCCAAA ACCCAAAACA GAGAAGAATC TTTTGACTTC
    TCCAGATCTT ATGAATACAA GTCAAACCCC TCAGCCGCTG CTGGTAACGA AATTCCAGGG
    GCATCTACCA AAGGTTGTCC TCCTCCTATT GCAGCGAAAC CCACTTTTGG ACGGCCTGTA
    CTGAAGCCCT CCACTCCCGT CCCTCCCCCA GAGAGTGAGG AGACGGGAGA GGGCAGTGAG
    GAGCAGCACA ATGCTCCCAA ATCTGTCCTG GGCAAAGTAA AAATATTTGA GAAGATGGAT
    CACAAGGCAA AGTTACAGAG AATGCAAGAG CTCCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACACACAA GCCGGACCCT
    GGCCCACCCC AGTACACAAG TTCCAGGCCC CCCGAGCCAC AGAAAGGCCC TGCCAGACTT
    TATCAGGATC CCAGAGGAAG CTATGGCAGT GATGCTGAGG AGGAGGAATA CCGGCAGCAG
    CTGTCAGAAC ACTCAAGGCA CGGTTATTAC GGCCAGCCTT CCCGATACCG GGACACGGAA
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851262.1
    CDS26..3451
    Translation

    Target ORF information:

    RefSeq Version XM_024995494.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCACA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TCTGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCAGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGGCCAAA ACCCAAAACA GAGAAGAATC TTTTGACTTC
    TCCAGATCTT ATGAATACAA GTCAAACCCC TCAGCCGCTG CTGGTAACGA AATTCCAGGG
    GCATCTACCA AAGGTTGTCC TCCTCCTATT GCAGCGAAAC CCACTTTTGG ACGGCCTGTA
    CTGAAGCCCT CCACTCCCGT CCCTCCCCCA GAGAGTGAGG AGACGGGAGA GGGCAGTGAG
    GAGCAGCACA ATGCTCCCAA ATCTGTCCTG GGCAAAGTAA AAATATTTGA GAAGATGGAT
    CACAAGGCAA AGTTACAGAG AATGCAAGAG CTCCAAGAAG CACAGAATGC AAGGATTGAA
    ATTGCTCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACACACAA GCCGGACCCT
    GGCCCACCCC AGTACACAAG TTCCAGGCCC CCCGAGCCAC AGAAAGGCCC TGCCAGACTT
    TATCAGGATC CCAGAGGAAG CTATGGCAGT GATGCTGAGG AGGAGGAATA CCGGCAGCAG
    CTGTCAGAAC ACTCAAGGCA CGGTTATTAC GGCCAGCCTT CCCGATACCG GGACACGGAA
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236945
    Clone ID Related Accession (Same CDS sequence) XM_024995495.1
    Accession Version XM_024995495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGGCCAAAA CCCAAAACAG AGAAGAATCT TTTGACTTCT CCAGATCTTA TGAATACAAG
    TCAAACCCCT CAGCCGCTGC TGGTAACGAA ATTCCAGGGG CATCTACCAA AGGTTGTCCT
    CCTCCTATTG CAGCGAAACC CACTTTTGGA CGGCCTGTAC TGAAGCCCTC CACTCCCGTC
    CCTCCCCCAG AGAGTGAGGA GACGGGAGAG GGCAGTGAGG AGCAGCACAA TGCTCCCAAA
    TCTGTCCTGG GCAAAGTAAA AATATTTGAG AAGATGGATC ACAAGGCAAA GTTACAGAGA
    ATGCAAGAGC TCCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACACACAAG CCGGACCCTG GCCCACCCCA GTACACAAGT
    TCCAGGCCCC CCGAGCCACA GAAAGGCCCT GCCAGACTTT ATCAGGATCC CAGAGGAAGC
    TATGGCAGTG ATGCTGAGGA GGAGGAATAC CGGCAGCAGC TGTCAGAACA CTCAAGGCAC
    GGTTATTACG GCCAGCCTTC CCGATACCGG GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851263.1
    CDS427..3771
    Translation

    Target ORF information:

    RefSeq Version XM_024995495.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGGCCAAAA CCCAAAACAG AGAAGAATCT TTTGACTTCT CCAGATCTTA TGAATACAAG
    TCAAACCCCT CAGCCGCTGC TGGTAACGAA ATTCCAGGGG CATCTACCAA AGGTTGTCCT
    CCTCCTATTG CAGCGAAACC CACTTTTGGA CGGCCTGTAC TGAAGCCCTC CACTCCCGTC
    CCTCCCCCAG AGAGTGAGGA GACGGGAGAG GGCAGTGAGG AGCAGCACAA TGCTCCCAAA
    TCTGTCCTGG GCAAAGTAAA AATATTTGAG AAGATGGATC ACAAGGCAAA GTTACAGAGA
    ATGCAAGAGC TCCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACACACAAG CCGGACCCTG GCCCACCCCA GTACACAAGT
    TCCAGGCCCC CCGAGCCACA GAAAGGCCCT GCCAGACTTT ATCAGGATCC CAGAGGAAGC
    TATGGCAGTG ATGCTGAGGA GGAGGAATAC CGGCAGCAGC TGTCAGAACA CTCAAGGCAC
    GGTTATTACG GCCAGCCTTC CCGATACCGG GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236946
    Clone ID Related Accession (Same CDS sequence) XM_024995496.1
    Accession Version XM_024995496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCACA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TCTGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCAGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851264.1
    CDS23..3118
    Translation

    Target ORF information:

    RefSeq Version XM_024995496.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCACA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TCTGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCAGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236947
    Clone ID Related Accession (Same CDS sequence) XM_024995497.1
    Accession Version XM_024995497.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3084bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGGTGC GAGGAGATCG CGGGTTTCCA TCTCGGCGGG AAATGTCAGG TTGGCTCCCT 
    GCTCCAGGCA TGGAAGAACT GATATGGGAA CAGTACACTG TGACCCTACA GAAGGATTCC
    AAGAGAGGAT TTGGAATTGC AGTATCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACATCCA TCGTCATTTC TGATGTGCTC CCGGGTGGGC CCGCCGATGG GCTGCTTCAA
    GAGAATGACA GGGTGGTCAT GGTTAATGGC ACCCCTATGG AAGACGTGCT CCATTCATTT
    GCGGTTCAGC AGCTGAGGAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCT GCAGGACAGT CCTCCTGTCC ACGAAGATGA CCGGGCTTTC
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGC GCCCGCAGCG GCTACAGCGA GAGGAGCCGG
    CCCAGCAGCC GCGGAGGGCG CAGCCGCAGC TGGGAGGAGA GCCCAGAGAG GGGCCGTCCC
    CATGACCGGG TGCGCAGCCG GGATCGAGGC CGGAGTCTGG AGCGGGGCCT GGACCACGAC
    GACGACTACG GGCGAGCCCG GGAGCACAGC CGTGGCCGCA GCCTCGAGCG GGGCCTAGAC
    CAAGATGATT ACAGGCGAGC CCGGGAGAGC AGCCGCGGCC GAAGCATTGA CCCGGTCTAC
    GATCGCGCCT ACACCCCTGA GGGGGAGTAC GGCCACAGGG CCCAGCCTGA TGCCCGGTAT
    GGGGCATCCC AGAGCCGCAG CCGTGAGCAC CTCCACTCCC GCAGCCCCAG CCCGGAGCCC
    CGGGGGCGTC CGGACTCGGA CAAGCCTATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAATATG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTAGCAACTA AAGATGGCAA CCTGCATGAA GGAGACATAA TTCTCAAGAT AAACGGAACC
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAACTGA TAGAAAAGTC AAGAGGGAAA
    CTCCAGCTCG TGGTGCTGAG GGACAGCAAA CAGACACTTA TCAACATCCC CTCATTAAAT
    GACAGCGACT CAGAAATAGA AGATATCTCA GAAATAGAGT CCAACCGATC GTTTTCTCCA
    GAGGAGAGGC AGCAGCAGTA TTCTGATTAT GATTACCATT CCTCAAATGA AAAGCTAAAG
    GAGAGACCAA ATTCAAGAGA GGACATGCAG AGCAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCACTGG GGATATGGCG GCTGCGGGCA GCGTGGAGCC CAGCAAGGAA
    CCCAAATACC AAGAGGAAGC CCCAGTTCCT CCACCAAAAC CAGCCCCGAG AGCTGTTCTT
    CGTCCAAGTC CTGAAGATTT AGCAATATAT GGCCCCAATA CCAAAATGGT GAGGTTCAAG
    AAGGGAGACA GTGTGGGCCT CCGGCTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCC
    GGCATTCAAG AGGGTACCTC CGCGGAGCAA GAAGGTCTTC AAGAAGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGACTC GTTCGGGAGG ATGCTGTTCT CTACCTGTTA
    GAAATCCCCA AAGGTGAAAC GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATACTGG CTTGTGGCAG AGGGGATTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGTGT GGTAGATACA
    CTCTACGATG GCAAACTGGG CCACTGGCTG GCCGTGAGGA TCGGGAACGA ACTGGAGAAA
    GGCTTAATCC CCAACAAAAG CAGAGCGGAA CAAATGGCCA GCGTTCAGAA TGCCCAGAGA
    GACACCACCG GGGACAGGGC AGACTTCTGG AGGATGCGCG GCCAGAGATC AGGTGTGAAG
    AAGAACCTAC GGAAGAGTCG GGAGGACCTG ACTGCCGCCG TGACAGTCAG CACCAAGTTC
    CCGGCCTACG AGAGGGTTTT GCTTCGAGAA GCTGGTTTCA AGAGACCTGT TGTCTTATTT
    GGCCCCATAG CAGATATAGC CCTGGAGAAG TTGGCAAATG ACTTGCCTGA CCTGTTCCAA
    ACTGCTAAAA CAGAACCAAG AGACGCAGGA TCTGAGAAAT CCACCGGGGT GGTGCGGTTA
    AATACCGTGA GACAAATTAT TGAACAGGAT AAGCATGCAC TCCTGGATGT AACTCCTAAG
    GCTGTGGACC TGTTGAACTA TACTCAGTGG TTCCCGATTG TGATCTTTTT CAACCCAGAC
    TCTAGGCAAG GTGTCAAAAC TATGAGACAG AGGTTGAATC CCTCGTCCAA CAAAAGTTCT
    CGGAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACTT GTGCACATCT TTTCACAGCT
    ACAATCAACC TCAATTCAGC CAACGACAGC TGGTTTGGCA GCTTGAAAGA CACGATTCAG
    CATCAGCAAG GAGAAGCAGT CTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAT
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCGCTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGATTGAAAT TGCTCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACACACAAGC CGGACCCTGG CCCACCCCAG TACACAAGTT CCAGGCCCCC CGAGCCACAG
    AAAGGCCCTG CCAGACTTTA TCAGGATCCC AGAGGAAGCT ATGGCAGTGA TGCTGAGGAG
    GAGGAATACC GGCAGCAGCT GTCAGAACAC TCAAGGCACG GTTATTACGG CCAGCCTTCC
    CGATACCGGG ACACGGAATT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851265.1
    CDS137..3220
    Translation

    Target ORF information:

    RefSeq Version XM_024995497.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGGTGC GAGGAGATCG CGGGTTTCCA TCTCGGCGGG AAATGTCAGG TTGGCTCCCT 
    GCTCCAGGCA TGGAAGAACT GATATGGGAA CAGTACACTG TGACCCTACA GAAGGATTCC
    AAGAGAGGAT TTGGAATTGC AGTATCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACATCCA TCGTCATTTC TGATGTGCTC CCGGGTGGGC CCGCCGATGG GCTGCTTCAA
    GAGAATGACA GGGTGGTCAT GGTTAATGGC ACCCCTATGG AAGACGTGCT CCATTCATTT
    GCGGTTCAGC AGCTGAGGAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCT GCAGGACAGT CCTCCTGTCC ACGAAGATGA CCGGGCTTTC
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGC GCCCGCAGCG GCTACAGCGA GAGGAGCCGG
    CCCAGCAGCC GCGGAGGGCG CAGCCGCAGC TGGGAGGAGA GCCCAGAGAG GGGCCGTCCC
    CATGACCGGG TGCGCAGCCG GGATCGAGGC CGGAGTCTGG AGCGGGGCCT GGACCACGAC
    GACGACTACG GGCGAGCCCG GGAGCACAGC CGTGGCCGCA GCCTCGAGCG GGGCCTAGAC
    CAAGATGATT ACAGGCGAGC CCGGGAGAGC AGCCGCGGCC GAAGCATTGA CCCGGTCTAC
    GATCGCGCCT ACACCCCTGA GGGGGAGTAC GGCCACAGGG CCCAGCCTGA TGCCCGGTAT
    GGGGCATCCC AGAGCCGCAG CCGTGAGCAC CTCCACTCCC GCAGCCCCAG CCCGGAGCCC
    CGGGGGCGTC CGGACTCGGA CAAGCCTATC GGGGTCCTTC TGATGAAAAG CAAAGCAAAT
    GAAGAATATG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTAGCAACTA AAGATGGCAA CCTGCATGAA GGAGACATAA TTCTCAAGAT AAACGGAACC
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAACTGA TAGAAAAGTC AAGAGGGAAA
    CTCCAGCTCG TGGTGCTGAG GGACAGCAAA CAGACACTTA TCAACATCCC CTCATTAAAT
    GACAGCGACT CAGAAATAGA AGATATCTCA GAAATAGAGT CCAACCGATC GTTTTCTCCA
    GAGGAGAGGC AGCAGCAGTA TTCTGATTAT GATTACCATT CCTCAAATGA AAAGCTAAAG
    GAGAGACCAA ATTCAAGAGA GGACATGCAG AGCAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCACTGG GGATATGGCG GCTGCGGGCA GCGTGGAGCC CAGCAAGGAA
    CCCAAATACC AAGAGGAAGC CCCAGTTCCT CCACCAAAAC CAGCCCCGAG AGCTGTTCTT
    CGTCCAAGTC CTGAAGATTT AGCAATATAT GGCCCCAATA CCAAAATGGT GAGGTTCAAG
    AAGGGAGACA GTGTGGGCCT CCGGCTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCC
    GGCATTCAAG AGGGTACCTC CGCGGAGCAA GAAGGTCTTC AAGAAGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGACTC GTTCGGGAGG ATGCTGTTCT CTACCTGTTA
    GAAATCCCCA AAGGTGAAAC GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATACTGG CTTGTGGCAG AGGGGATTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGTGT GGTAGATACA
    CTCTACGATG GCAAACTGGG CCACTGGCTG GCCGTGAGGA TCGGGAACGA ACTGGAGAAA
    GGCTTAATCC CCAACAAAAG CAGAGCGGAA CAAATGGCCA GCGTTCAGAA TGCCCAGAGA
    GACACCACCG GGGACAGGGC AGACTTCTGG AGGATGCGCG GCCAGAGATC AGGTGTGAAG
    AAGAACCTAC GGAAGAGTCG GGAGGACCTG ACTGCCGCCG TGACAGTCAG CACCAAGTTC
    CCGGCCTACG AGAGGGTTTT GCTTCGAGAA GCTGGTTTCA AGAGACCTGT TGTCTTATTT
    GGCCCCATAG CAGATATAGC CCTGGAGAAG TTGGCAAATG ACTTGCCTGA CCTGTTCCAA
    ACTGCTAAAA CAGAACCAAG AGACGCAGGA TCTGAGAAAT CCACCGGGGT GGTGCGGTTA
    AATACCGTGA GACAAATTAT TGAACAGGAT AAGCATGCAC TCCTGGATGT AACTCCTAAG
    GCTGTGGACC TGTTGAACTA TACTCAGTGG TTCCCGATTG TGATCTTTTT CAACCCAGAC
    TCTAGGCAAG GTGTCAAAAC TATGAGACAG AGGTTGAATC CCTCGTCCAA CAAAAGTTCT
    CGGAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACTT GTGCACATCT TTTCACAGCT
    ACAATCAACC TCAATTCAGC CAACGACAGC TGGTTTGGCA GCTTGAAAGA CACGATTCAG
    CATCAGCAAG GAGAAGCAGT CTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAT
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCGCTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGATTGAAAT TGCTCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACACACAAGC CGGACCCTGG CCCACCCCAG TACACAAGTT CCAGGCCCCC CGAGCCACAG
    AAAGGCCCTG CCAGACTTTA TCAGGATCCC AGAGGAAGCT ATGGCAGTGA TGCTGAGGAG
    GAGGAATACC GGCAGCAGCT GTCAGAACAC TCAAGGCACG GTTATTACGG CCAGCCTTCC
    CGATACCGGG ACACGGAATT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236948
    Clone ID Related Accession (Same CDS sequence) XM_024995499.1
    Accession Version XM_024995499.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGATTGAAA TTGCTCAGAA GCATCCTGAT ATCTATGCAG TTCCAATCAA AACACACAAG
    CCGGACCCTG GCCCACCCCA GTACACAAGT TCCAGGCCCC CCGAGCCACA GAAAGGCCCT
    GCCAGACTTT ATCAGGATCC CAGAGGAAGC TATGGCAGTG ATGCTGAGGA GGAGGAATAC
    CGGCAGCAGC TGTCAGAACA CTCAAGGCAC GGTTATTACG GCCAGCCTTC CCGATACCGG
    GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851267.1
    CDS567..3581
    Translation

    Target ORF information:

    RefSeq Version XM_024995499.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995499.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCACAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CAGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGATTGAAA TTGCTCAGAA GCATCCTGAT ATCTATGCAG TTCCAATCAA AACACACAAG
    CCGGACCCTG GCCCACCCCA GTACACAAGT TCCAGGCCCC CCGAGCCACA GAAAGGCCCT
    GCCAGACTTT ATCAGGATCC CAGAGGAAGC TATGGCAGTG ATGCTGAGGA GGAGGAATAC
    CGGCAGCAGC TGTCAGAACA CTCAAGGCAC GGTTATTACG GCCAGCCTTC CCGATACCGG
    GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39431
    Clone ID Related Accession (Same CDS sequence) NM_001102482.1
    Accession Version NM_001102482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149801.1. On Aug 29, 2007 this sequence version replaced XM_873993.2. ##Evidence-Data-START## Transcript exon combination :: BC149801.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCGCAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CGGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCAGA GAGACACCAC CGGGGACAGG
    GCAGACTTCT GGAGGATGCG CGGCCAGAGA TCAGGTGTGA AGAAGAACCT ACGGAAGAGT
    CGGGAGGACC TGACTGCCGC CGTGACAGTC AGCACCAAGT TCCCGGCCTA CGAGAGGGTT
    TTGCTTCGAG AAGCTGGTTT CAAGAGACCT GTTGTCTTAT TTGGCCCCAT AGCAGATATA
    GCCCTGGAGA AGTTGGCAAA TGACTTGCCT GACCTGTTCC AAACTGCTAA AACAGAACCA
    AGAGACGCAG GATCTGAGAA ATCCACCGGG GTGGTGCGGT TAAATACCGT GAGACAAATT
    ATTGAACAGG ATAAGCATGC ACTCCTGGAT GTAACTCCTA AGGCTGTGGA CCTGTTGAAC
    TATACTCAGT GGTTCCCGAT TGTGATCTTT TTCAACCCAG ACTCTAGGCA AGGTGTCAAA
    ACTATGAGAC AGAGGTTGAA TCCCTCGTCC AACAAAAGTT CTCGGAAGTT ATTTGATCAA
    GCCAACAAGC TTAAAAAAAC TTGTGCACAT CTTTTCACAG CTACAATCAA CCTCAATTCA
    GCCAACGACA GCTGGTTTGG CAGCTTGAAA GACACGATTC AGCATCAGCA AGGAGAAGCA
    GTCTGGGTCT CTGAAGGAAA GATGGAAGGG ATGGATGATG ATCCCGAAGA CCGCATGTCC
    TACTTAACCG CCATGGGCGC GGACTATCTG AGTTGCGACA GCCGCCTCAT CAGTGACTTT
    GAAGACACCG ACGGTGAAGG AGGTGCCTAC ACTGACAATG AGCTGGATGA GCCAGCTGAG
    GAGCCGCTGG TGTCTTCCAT CACCCGCTCC TCGGAGCCGG TGCAGCATGA GGAGATTGAA
    ATTGCTCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACACACAA GCCGGACCCT
    GGCCCACCCC AGTACACAAG TTCCAGGCCC CCCGAGCCAC AGAAAGGCCC TGCCAGACTT
    TATCAGGATC CCAGAGGAAG CTATGGCAGT GATGCTGAGG AGGAGGAATA CCGGCAGCAG
    CTGTCAGAAC ACTCAAGGCA CGGTTATTAC GGCCAGCCTT CCCGATACCG GGACACGGAA
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095952.1
    CDS44..2509
    Translation

    Target ORF information:

    RefSeq Version NM_001102482.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCGCAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT CTGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CGGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCAGA GAGACACCAC CGGGGACAGG
    GCAGACTTCT GGAGGATGCG CGGCCAGAGA TCAGGTGTGA AGAAGAACCT ACGGAAGAGT
    CGGGAGGACC TGACTGCCGC CGTGACAGTC AGCACCAAGT TCCCGGCCTA CGAGAGGGTT
    TTGCTTCGAG AAGCTGGTTT CAAGAGACCT GTTGTCTTAT TTGGCCCCAT AGCAGATATA
    GCCCTGGAGA AGTTGGCAAA TGACTTGCCT GACCTGTTCC AAACTGCTAA AACAGAACCA
    AGAGACGCAG GATCTGAGAA ATCCACCGGG GTGGTGCGGT TAAATACCGT GAGACAAATT
    ATTGAACAGG ATAAGCATGC ACTCCTGGAT GTAACTCCTA AGGCTGTGGA CCTGTTGAAC
    TATACTCAGT GGTTCCCGAT TGTGATCTTT TTCAACCCAG ACTCTAGGCA AGGTGTCAAA
    ACTATGAGAC AGAGGTTGAA TCCCTCGTCC AACAAAAGTT CTCGGAAGTT ATTTGATCAA
    GCCAACAAGC TTAAAAAAAC TTGTGCACAT CTTTTCACAG CTACAATCAA CCTCAATTCA
    GCCAACGACA GCTGGTTTGG CAGCTTGAAA GACACGATTC AGCATCAGCA AGGAGAAGCA
    GTCTGGGTCT CTGAAGGAAA GATGGAAGGG ATGGATGATG ATCCCGAAGA CCGCATGTCC
    TACTTAACCG CCATGGGCGC GGACTATCTG AGTTGCGACA GCCGCCTCAT CAGTGACTTT
    GAAGACACCG ACGGTGAAGG AGGTGCCTAC ACTGACAATG AGCTGGATGA GCCAGCTGAG
    GAGCCGCTGG TGTCTTCCAT CACCCGCTCC TCGGAGCCGG TGCAGCATGA GGAGATTGAA
    ATTGCTCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACACACAA GCCGGACCCT
    GGCCCACCCC AGTACACAAG TTCCAGGCCC CCCGAGCCAC AGAAAGGCCC TGCCAGACTT
    TATCAGGATC CCAGAGGAAG CTATGGCAGT GATGCTGAGG AGGAGGAATA CCGGCAGCAG
    CTGTCAGAAC ACTCAAGGCA CGGTTATTAC GGCCAGCCTT CCCGATACCG GGACACGGAA
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21973
    Clone ID Related Accession (Same CDS sequence) XM_005210007.3
    Accession Version XM_005210007.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCGCA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TATGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCGGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGAGCATA AGGAAACCCA GCCCAGAGCC ACGAGCTCAG
    ATGAGGAGGG CTGCTAGCAG AGATCAACTT AGGGACAATA GCCCGCCCCC GACATTCAAG
    CCAGAGCCGC CCAAGGCCAA AACCCAAAAC AGAGAAGAAT CTTTTGACTT CTCCAGATCT
    TATGAATACA AGTCAAACCC CTCAGCCGCT GCTGGTAACG AAATTCCAGG GGCATCTACC
    AAAGGTTGTC CTCCTCCTAT TGCAGCGAAA CCCACTTTTG GACGGCCTGT ACTGAAGCCC
    TCCACTCCCG TCCCTCCCCC AGAGAGTGAG GAGACGGGAG AGGGCAGTGA GGAGCAGCAC
    AATGCTCCCA AATCTGTCCT GGGCAAAGTA AAAATATTTG AGAAGATGGA TCACAAGGCA
    AAGTTACAGA GAATGCAAGA GCTCCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ATATCTATGC AGTTCCAATC AAAACACACA AGCCGGACCC TGGCCCACCC
    CAGTACACAA GTTCCAGGCC CCCCGAGCCA CAGAAAGGCC CTGCCAGACT TTATCAGGAT
    CCCAGAGGAA GCTATGGCAG TGATGCTGAG GAGGAGGAAT ACCGGCAGCA GCTGTCAGAA
    CACTCAAGGC ACGGTTATTA CGGCCAGCCT TCCCGATACC GGGACACGGA ATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210064.1
    CDS22..3558
    Translation

    Target ORF information:

    RefSeq Version XM_005210007.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGACCG CACAGTTCCT CCAGAGGATG TGGGTCCAGG CTGTTAAAAA GTTGGGGAGA 
    TTGAAAGGCC ATGCTCCAGG CATGGAAGAA CTGATATGGG AACAGTACAC TGTGACCCTA
    CAGAAGGATT CCAAGAGAGG ATTTGGAATT GCAGTATCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACATC CATCGTCATT TCTGATGTGC TCCCGGGTGG GCCCGCCGAT
    GGGCTGCTTC AAGAGAATGA CAGGGTGGTC ATGGTTAATG GCACCCCTAT GGAAGACGTG
    CTCCATTCAT TTGCGGTTCA GCAGCTGAGG AAAAGTGGGA AGATCGCTGC CATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GCTGGCCCCG CTGCAGGACA GTCCTCCTGT CCACGAAGAT
    GACCGGGCTT TCGAGGTGAT GGACGAGTTT GATGGCAGAA GCGCCCGCAG CGGCTACAGC
    GAGAGGAGCC GGCCCAGCAG CCGCGGAGGG CGCAGCCGCA GCTGGGAGGA GAGCCCAGAG
    AGGGGCCGTC CCCATGACCG GGTGCGCAGC CGGGATCGAG GCCGGAGTCT GGAGCGGGGC
    CTGGACCACG ACGACGACTA CGGGCGAGCC CGGGAGCGCA GCCGTGGCCG CAGCCTCGAG
    CGGGGCCTAG ACCAAGATGA TTACAGGCGA GCCCGGGAGA GCAGCCGCGG CCGAAGCATT
    GACCCGGTCT ACGATCGCGC CTACACCCCT GAGGGGGAGT ACGGCCACAG GGCCCAGCCT
    GATGCCCGGT ATGGGGCATC CCAGAGCCGC AGCCGTGAGC ACCTCCACTC CCGCAGCCCC
    AGCCCGGAGC CCCGGGGGCG TCCGGACTCG GACAAGCCTA TCGGGGTCCT TATGATGAAA
    AGCAAAGCAA ATGAAGAATA TGGTCTCCGG CTTGGGAGTC AGATCTTCAT AAAGGAAATG
    ACCAGAACCG GTCTAGCAAC TAAAGATGGC AACCTGCATG AAGGAGACAT AATTCTCAAG
    ATAAACGGAA CCGTAACTGA GAACATGTCT TTAACGGATG CTCGAAAACT GATAGAAAAG
    TCAAGAGGGA AACTCCAGCT CGTGGTGCTG AGGGACAGCA AACAGACACT TATCAACATC
    CCCTCATTAA ATGACAGCGA CTCAGAAATA GAAGATATCT CAGAAATAGA GTCCAACCGA
    TCGTTTTCTC CAGAGGAGAG GCGGCAGCAG TATTCTGATT ATGATTACCA TTCCTCAAAT
    GAAAAGCTAA AGGAGAGACC AAATTCAAGA GAGGACATGC AGAGCAGATG GTCCAGGATG
    GGCGCCACAC CCACTCCCTT TAAGTCCACT GGGGATATGG CGGCTGCGGG CAGCGTGGAG
    CCCAGCAAGG AACCCAAATA CCAAGAGGAA GCCCCAGTTC CTCCACCAAA ACCAGCCCCG
    AGAGCTGTTC TTCGTCCAAG TCCTGAAGAT TTAGCAATAT ATGGCCCCAA TACCAAAATG
    GTGAGGTTCA AGAAGGGAGA CAGTGTGGGC CTCCGGCTGG CTGGTGGCAA TGACGTTGGG
    ATATTTGTGG CCGGCATTCA AGAGGGTACC TCCGCGGAGC AAGAAGGTCT TCAAGAAGGG
    GACCAGATTC TGAAGGTGAA CACACAGGAT TTCAGAGGAC TCGTTCGGGA GGATGCTGTT
    CTCTACCTGT TAGAAATCCC CAAAGGTGAA ACGGTGACCA TTTTAGCTCA GAGCCGAGCT
    GATGTGTATA GAGACATACT GGCTTGTGGC AGAGGGGATT CATTTTTTAT AAGAAGCCAC
    TTTGAATGTG AGAAGGAAAC TCCACAGAGC CTGGCCTTCA CCAGAGGGGA GGTCTTCCGT
    GTGGTAGATA CACTCTACGA TGGCAAACTG GGCCACTGGC TGGCCGTGAG GATCGGGAAC
    GAACTGGAGA AAGGCTTAAT CCCCAACAAA AGCAGAGCGG AACAAATGGC CAGCGTTCAG
    AATGCCCAGA GAGACACCAC CGGGGACAGG GCAGACTTCT GGAGGATGCG CGGCCAGAGA
    TCAGGTGTGA AGAAGAACCT ACGGAAGAGT CGGGAGGACC TGACTGCCGC CGTGACAGTC
    AGCACCAAGT TCCCGGCCTA CGAGAGGGTT TTGCTTCGAG AAGCTGGTTT CAAGAGACCT
    GTTGTCTTAT TTGGCCCCAT AGCAGATATA GCCCTGGAGA AGTTGGCAAA TGACTTGCCT
    GACCTGTTCC AAACTGCTAA AACAGAACCA AGAGACGCAG GATCTGAGAA ATCCACCGGG
    GTGGTGCGGT TAAATACCGT GAGACAAATT ATTGAACAGG ATAAGCATGC ACTCCTGGAT
    GTAACTCCTA AGGCTGTGGA CCTGTTGAAC TATACTCAGT GGTTCCCGAT TGTGATCTTT
    TTCAACCCAG ACTCTAGGCA AGGTGTCAAA ACTATGAGAC AGAGGTTGAA TCCCTCGTCC
    AACAAAAGTT CTCGGAAGTT ATTTGATCAA GCCAACAAGC TTAAAAAAAC TTGTGCACAT
    CTTTTCACAG CTACAATCAA CCTCAATTCA GCCAACGACA GCTGGTTTGG CAGCTTGAAA
    GACACGATTC AGCATCAGCA AGGAGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG
    ATGGATGATG ATCCCGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG
    AGTTGCGACA GCCGCCTCAT CAGTGACTTT GAAGACACCG ACGGTGAAGG AGGTGCCTAC
    ACTGACAATG AGCTGGATGA GCCAGCTGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC
    TCGGAGCCGG TGCAGCATGA GGAGAGCATA AGGAAACCCA GCCCAGAGCC ACGAGCTCAG
    ATGAGGAGGG CTGCTAGCAG AGATCAACTT AGGGACAATA GCCCGCCCCC GACATTCAAG
    CCAGAGCCGC CCAAGGCCAA AACCCAAAAC AGAGAAGAAT CTTTTGACTT CTCCAGATCT
    TATGAATACA AGTCAAACCC CTCAGCCGCT GCTGGTAACG AAATTCCAGG GGCATCTACC
    AAAGGTTGTC CTCCTCCTAT TGCAGCGAAA CCCACTTTTG GACGGCCTGT ACTGAAGCCC
    TCCACTCCCG TCCCTCCCCC AGAGAGTGAG GAGACGGGAG AGGGCAGTGA GGAGCAGCAC
    AATGCTCCCA AATCTGTCCT GGGCAAAGTA AAAATATTTG AGAAGATGGA TCACAAGGCA
    AAGTTACAGA GAATGCAAGA GCTCCAAGAA GCACAGAATG CAAGGATTGA AATTGCTCAG
    AAGCATCCTG ATATCTATGC AGTTCCAATC AAAACACACA AGCCGGACCC TGGCCCACCC
    CAGTACACAA GTTCCAGGCC CCCCGAGCCA CAGAAAGGCC CTGCCAGACT TTATCAGGAT
    CCCAGAGGAA GCTATGGCAG TGATGCTGAG GAGGAGGAAT ACCGGCAGCA GCTGTCAGAA
    CACTCAAGGC ACGGTTATTA CGGCCAGCCT TCCCGATACC GGGACACGGA ATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21974
    Clone ID Related Accession (Same CDS sequence) XM_005210008.1
    Accession Version XM_005210008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGC GAGGAGATCG CGGGTTTCCA TCTCGGCGGG AAATGTCAGG TTGGCTCCCT 
    GCTCCAGGCA TGGAAGAACT GATATGGGAA CAGTACACTG TGACCCTACA GAAGGATTCC
    AAGAGAGGAT TTGGAATTGC AGTATCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACATCCA TCGTCATTTC TGATGTGCTC CCGGGTGGGC CCGCCGATGG GCTGCTTCAA
    GAGAATGACA GGGTGGTCAT GGTTAATGGC ACCCCTATGG AAGACGTGCT CCATTCATTT
    GCGGTTCAGC AGCTGAGGAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCT GCAGGACAGT CCTCCTGTCC ACGAAGATGA CCGGGCTTTC
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGC GCCCGCAGCG GCTACAGCGA GAGGAGCCGG
    CCCAGCAGCC GCGGAGGGCG CAGCCGCAGC TGGGAGGAGA GCCCAGAGAG GGGCCGTCCC
    CATGACCGGG TGCGCAGCCG GGATCGAGGC CGGAGTCTGG AGCGGGGCCT GGACCACGAC
    GACGACTACG GGCGAGCCCG GGAGCGCAGC CGTGGCCGCA GCCTCGAGCG GGGCCTAGAC
    CAAGATGATT ACAGGCGAGC CCGGGAGAGC AGCCGCGGCC GAAGCATTGA CCCGGTCTAC
    GATCGCGCCT ACACCCCTGA GGGGGAGTAC GGCCACAGGG CCCAGCCTGA TGCCCGGTAT
    GGGGCATCCC AGAGCCGCAG CCGTGAGCAC CTCCACTCCC GCAGCCCCAG CCCGGAGCCC
    CGGGGGCGTC CGGACTCGGA CAAGCCTATC GGGGTCCTTA TGATGAAAAG CAAAGCAAAT
    GAAGAATATG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTAGCAACTA AAGATGGCAA CCTGCATGAA GGAGACATAA TTCTCAAGAT AAACGGAACC
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAACTGA TAGAAAAGTC AAGAGGGAAA
    CTCCAGCTCG TGGTGCTGAG GGACAGCAAA CAGACACTTA TCAACATCCC CTCATTAAAT
    GACAGCGACT CAGAAATAGA AGATATCTCA GAAATAGAGT CCAACCGATC GTTTTCTCCA
    GAGGAGAGGC GGCAGCAGTA TTCTGATTAT GATTACCATT CCTCAAATGA AAAGCTAAAG
    GAGAGACCAA ATTCAAGAGA GGACATGCAG AGCAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCACTGG GGATATGGCG GCTGCGGGCA GCGTGGAGCC CAGCAAGGAA
    CCCAAATACC AAGAGGAAGC CCCAGTTCCT CCACCAAAAC CAGCCCCGAG AGCTGTTCTT
    CGTCCAAGTC CTGAAGATTT AGCAATATAT GGCCCCAATA CCAAAATGGT GAGGTTCAAG
    AAGGGAGACA GTGTGGGCCT CCGGCTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCC
    GGCATTCAAG AGGGTACCTC CGCGGAGCAA GAAGGTCTTC AAGAAGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGACTC GTTCGGGAGG ATGCTGTTCT CTACCTGTTA
    GAAATCCCCA AAGGTGAAAC GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATACTGG CTTGTGGCAG AGGGGATTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGTGT GGTAGATACA
    CTCTACGATG GCAAACTGGG CCACTGGCTG GCCGTGAGGA TCGGGAACGA ACTGGAGAAA
    GGCTTAATCC CCAACAAAAG CAGAGCGGAA CAAATGGCCA GCGTTCAGAA TGCCCAGAGA
    GACACCACCG GGGACAGGGC AGACTTCTGG AGGATGCGCG GCCAGAGATC AGGTGTGAAG
    AAGAACCTAC GGAAGAGTCG GGAGGACCTG ACTGCCGCCG TGACAGTCAG CACCAAGTTC
    CCGGCCTACG AGAGGGTTTT GCTTCGAGAA GCTGGTTTCA AGAGACCTGT TGTCTTATTT
    GGCCCCATAG CAGATATAGC CCTGGAGAAG TTGGCAAATG ACTTGCCTGA CCTGTTCCAA
    ACTGCTAAAA CAGAACCAAG AGACGCAGGA TCTGAGAAAT CCACCGGGGT GGTGCGGTTA
    AATACCGTGA GACAAATTAT TGAACAGGAT AAGCATGCAC TCCTGGATGT AACTCCTAAG
    GCTGTGGACC TGTTGAACTA TACTCAGTGG TTCCCGATTG TGATCTTTTT CAACCCAGAC
    TCTAGGCAAG GTGTCAAAAC TATGAGACAG AGGTTGAATC CCTCGTCCAA CAAAAGTTCT
    CGGAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACTT GTGCACATCT TTTCACAGCT
    ACAATCAACC TCAATTCAGC CAACGACAGC TGGTTTGGCA GCTTGAAAGA CACGATTCAG
    CATCAGCAAG GAGAAGCAGT CTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAT
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCGCTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAATAGC CCGCCCCCGA CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAAAACAG AGAAGAATCT TTTGACTTCT CCAGATCTTA TGAATACAAG
    TCAAACCCCT CAGCCGCTGC TGGTAACGAA ATTCCAGGGG CATCTACCAA AGGTTGTCCT
    CCTCCTATTG CAGCGAAACC CACTTTTGGA CGGCCTGTAC TGAAGCCCTC CACTCCCGTC
    CCTCCCCCAG AGAGTGAGGA GACGGGAGAG GGCAGTGAGG AGCAGCACAA TGCTCCCAAA
    TCTGTCCTGG GCAAAGTAAA AATATTTGAG AAGATGGATC ACAAGGCAAA GTTACAGAGA
    ATGCAAGAGC TCCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACACACAAG CCGGACCCTG GCCCACCCCA GTACACAAGT
    TCCAGGCCCC CCGAGCCACA GAAAGGCCCT GCCAGACTTT ATCAGGATCC CAGAGGAAGC
    TATGGCAGTG ATGCTGAGGA GGAGGAATAC CGGCAGCAGC TGTCAGAACA CTCAAGGCAC
    GGTTATTACG GCCAGCCTTC CCGATACCGG GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210065.1
    CDS117..3641
    Translation

    Target ORF information:

    RefSeq Version XM_005210008.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGC GAGGAGATCG CGGGTTTCCA TCTCGGCGGG AAATGTCAGG TTGGCTCCCT 
    GCTCCAGGCA TGGAAGAACT GATATGGGAA CAGTACACTG TGACCCTACA GAAGGATTCC
    AAGAGAGGAT TTGGAATTGC AGTATCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACATCCA TCGTCATTTC TGATGTGCTC CCGGGTGGGC CCGCCGATGG GCTGCTTCAA
    GAGAATGACA GGGTGGTCAT GGTTAATGGC ACCCCTATGG AAGACGTGCT CCATTCATTT
    GCGGTTCAGC AGCTGAGGAA AAGTGGGAAG ATCGCTGCCA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGC TGGCCCCGCT GCAGGACAGT CCTCCTGTCC ACGAAGATGA CCGGGCTTTC
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGC GCCCGCAGCG GCTACAGCGA GAGGAGCCGG
    CCCAGCAGCC GCGGAGGGCG CAGCCGCAGC TGGGAGGAGA GCCCAGAGAG GGGCCGTCCC
    CATGACCGGG TGCGCAGCCG GGATCGAGGC CGGAGTCTGG AGCGGGGCCT GGACCACGAC
    GACGACTACG GGCGAGCCCG GGAGCGCAGC CGTGGCCGCA GCCTCGAGCG GGGCCTAGAC
    CAAGATGATT ACAGGCGAGC CCGGGAGAGC AGCCGCGGCC GAAGCATTGA CCCGGTCTAC
    GATCGCGCCT ACACCCCTGA GGGGGAGTAC GGCCACAGGG CCCAGCCTGA TGCCCGGTAT
    GGGGCATCCC AGAGCCGCAG CCGTGAGCAC CTCCACTCCC GCAGCCCCAG CCCGGAGCCC
    CGGGGGCGTC CGGACTCGGA CAAGCCTATC GGGGTCCTTA TGATGAAAAG CAAAGCAAAT
    GAAGAATATG GTCTCCGGCT TGGGAGTCAG ATCTTCATAA AGGAAATGAC CAGAACCGGT
    CTAGCAACTA AAGATGGCAA CCTGCATGAA GGAGACATAA TTCTCAAGAT AAACGGAACC
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAACTGA TAGAAAAGTC AAGAGGGAAA
    CTCCAGCTCG TGGTGCTGAG GGACAGCAAA CAGACACTTA TCAACATCCC CTCATTAAAT
    GACAGCGACT CAGAAATAGA AGATATCTCA GAAATAGAGT CCAACCGATC GTTTTCTCCA
    GAGGAGAGGC GGCAGCAGTA TTCTGATTAT GATTACCATT CCTCAAATGA AAAGCTAAAG
    GAGAGACCAA ATTCAAGAGA GGACATGCAG AGCAGATGGT CCAGGATGGG CGCCACACCC
    ACTCCCTTTA AGTCCACTGG GGATATGGCG GCTGCGGGCA GCGTGGAGCC CAGCAAGGAA
    CCCAAATACC AAGAGGAAGC CCCAGTTCCT CCACCAAAAC CAGCCCCGAG AGCTGTTCTT
    CGTCCAAGTC CTGAAGATTT AGCAATATAT GGCCCCAATA CCAAAATGGT GAGGTTCAAG
    AAGGGAGACA GTGTGGGCCT CCGGCTGGCT GGTGGCAATG ACGTTGGGAT ATTTGTGGCC
    GGCATTCAAG AGGGTACCTC CGCGGAGCAA GAAGGTCTTC AAGAAGGGGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGACTC GTTCGGGAGG ATGCTGTTCT CTACCTGTTA
    GAAATCCCCA AAGGTGAAAC GGTGACCATT TTAGCTCAGA GCCGAGCTGA TGTGTATAGA
    GACATACTGG CTTGTGGCAG AGGGGATTCA TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGTGT GGTAGATACA
    CTCTACGATG GCAAACTGGG CCACTGGCTG GCCGTGAGGA TCGGGAACGA ACTGGAGAAA
    GGCTTAATCC CCAACAAAAG CAGAGCGGAA CAAATGGCCA GCGTTCAGAA TGCCCAGAGA
    GACACCACCG GGGACAGGGC AGACTTCTGG AGGATGCGCG GCCAGAGATC AGGTGTGAAG
    AAGAACCTAC GGAAGAGTCG GGAGGACCTG ACTGCCGCCG TGACAGTCAG CACCAAGTTC
    CCGGCCTACG AGAGGGTTTT GCTTCGAGAA GCTGGTTTCA AGAGACCTGT TGTCTTATTT
    GGCCCCATAG CAGATATAGC CCTGGAGAAG TTGGCAAATG ACTTGCCTGA CCTGTTCCAA
    ACTGCTAAAA CAGAACCAAG AGACGCAGGA TCTGAGAAAT CCACCGGGGT GGTGCGGTTA
    AATACCGTGA GACAAATTAT TGAACAGGAT AAGCATGCAC TCCTGGATGT AACTCCTAAG
    GCTGTGGACC TGTTGAACTA TACTCAGTGG TTCCCGATTG TGATCTTTTT CAACCCAGAC
    TCTAGGCAAG GTGTCAAAAC TATGAGACAG AGGTTGAATC CCTCGTCCAA CAAAAGTTCT
    CGGAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACTT GTGCACATCT TTTCACAGCT
    ACAATCAACC TCAATTCAGC CAACGACAGC TGGTTTGGCA GCTTGAAAGA CACGATTCAG
    CATCAGCAAG GAGAAGCAGT CTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAT
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACCGAC GGTGAAGGAG GTGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCTGAGGA GCCGCTGGTG TCTTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCATGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGAG ATCAACTTAG GGACAATAGC CCGCCCCCGA CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAAAACAG AGAAGAATCT TTTGACTTCT CCAGATCTTA TGAATACAAG
    TCAAACCCCT CAGCCGCTGC TGGTAACGAA ATTCCAGGGG CATCTACCAA AGGTTGTCCT
    CCTCCTATTG CAGCGAAACC CACTTTTGGA CGGCCTGTAC TGAAGCCCTC CACTCCCGTC
    CCTCCCCCAG AGAGTGAGGA GACGGGAGAG GGCAGTGAGG AGCAGCACAA TGCTCCCAAA
    TCTGTCCTGG GCAAAGTAAA AATATTTGAG AAGATGGATC ACAAGGCAAA GTTACAGAGA
    ATGCAAGAGC TCCAAGAAGC ACAGAATGCA AGGATTGAAA TTGCTCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACACACAAG CCGGACCCTG GCCCACCCCA GTACACAAGT
    TCCAGGCCCC CCGAGCCACA GAAAGGCCCT GCCAGACTTT ATCAGGATCC CAGAGGAAGC
    TATGGCAGTG ATGCTGAGGA GGAGGAATAC CGGCAGCAGC TGTCAGAACA CTCAAGGCAC
    GGTTATTACG GCCAGCCTTC CCGATACCGG GACACGGAAT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21975
    Clone ID Related Accession (Same CDS sequence) XM_010807869.2
    Accession Version XM_010807869.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCGCAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT ATGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CGGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGAGCATAA GGAAACCCAG CCCAGAGCCA CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA
    GATCAACTTA GGGACAATAG CCCGCCCCCG ACATTCAAGC CAGAGCCGCC CAAGGCCAAA
    ACCCAAAACA GAGAAGAATC TTTTGACTTC TCCAGATCTT ATGAATACAA GTCAAACCCC
    TCAGCCGCTG CTGGTAACGA AATTCCAGGG GCATCTACCA AAGGTTGTCC TCCTCCTATT
    GCAGCGAAAC CCACTTTTGG ACGGCCTGTA CTGAAGCCCT CCACTCCCGT CCCTCCCCCA
    GAGAGTGAGG AGACGGGAGA GGGCAGTGAG GAGCAGCACA ATGCTCCCAA ATCTGTCCTG
    GGCAAAGTAA AAATATTTGA GAAGATGGAT CACAAGGCAA AGTTACAGAG AATGCAAGAG
    CTCCAAGAAG CACAGAATGC AAGGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806171.1
    CDS569..4024
    Translation

    Target ORF information:

    RefSeq Version XM_010807869.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807869.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAAGAAC TGATATGGGA ACAGTACACT GTGACCCTAC AGAAGGATTC CAAGAGAGGA 
    TTTGGAATTG CAGTATCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACATCC
    ATCGTCATTT CTGATGTGCT CCCGGGTGGG CCCGCCGATG GGCTGCTTCA AGAGAATGAC
    AGGGTGGTCA TGGTTAATGG CACCCCTATG GAAGACGTGC TCCATTCATT TGCGGTTCAG
    CAGCTGAGGA AAAGTGGGAA GATCGCTGCC ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    CTGGCCCCGC TGCAGGACAG TCCTCCTGTC CACGAAGATG ACCGGGCTTT CGAGGTGATG
    GACGAGTTTG ATGGCAGAAG CGCCCGCAGC GGCTACAGCG AGAGGAGCCG GCCCAGCAGC
    CGCGGAGGGC GCAGCCGCAG CTGGGAGGAG AGCCCAGAGA GGGGCCGTCC CCATGACCGG
    GTGCGCAGCC GGGATCGAGG CCGGAGTCTG GAGCGGGGCC TGGACCACGA CGACGACTAC
    GGGCGAGCCC GGGAGCGCAG CCGTGGCCGC AGCCTCGAGC GGGGCCTAGA CCAAGATGAT
    TACAGGCGAG CCCGGGAGAG CAGCCGCGGC CGAAGCATTG ACCCGGTCTA CGATCGCGCC
    TACACCCCTG AGGGGGAGTA CGGCCACAGG GCCCAGCCTG ATGCCCGGTA TGGGGCATCC
    CAGAGCCGCA GCCGTGAGCA CCTCCACTCC CGCAGCCCCA GCCCGGAGCC CCGGGGGCGT
    CCGGACTCGG ACAAGCCTAT CGGGGTCCTT ATGATGAAAA GCAAAGCAAA TGAAGAATAT
    GGTCTCCGGC TTGGGAGTCA GATCTTCATA AAGGAAATGA CCAGAACCGG TCTAGCAACT
    AAAGATGGCA ACCTGCATGA AGGAGACATA ATTCTCAAGA TAAACGGAAC CGTAACTGAG
    AACATGTCTT TAACGGATGC TCGAAAACTG ATAGAAAAGT CAAGAGGGAA ACTCCAGCTC
    GTGGTGCTGA GGGACAGCAA ACAGACACTT ATCAACATCC CCTCATTAAA TGACAGCGAC
    TCAGAAATAG AAGATATCTC AGAAATAGAG TCCAACCGAT CGTTTTCTCC AGAGGAGAGG
    CGGCAGCAGT ATTCTGATTA TGATTACCAT TCCTCAAATG AAAAGCTAAA GGAGAGACCA
    AATTCAAGAG AGGACATGCA GAGCAGATGG TCCAGGATGG GCGCCACACC CACTCCCTTT
    AAGTCCACTG GGGATATGGC GGCTGCGGGC AGCGTGGAGC CCAGCAAGGA ACCCAAATAC
    CAAGAGGAAG CCCCAGTTCC TCCACCAAAA CCAGCCCCGA GAGCTGTTCT TCGTCCAAGT
    CCTGAAGATT TAGCAATATA TGGCCCCAAT ACCAAAATGG TGAGGTTCAA GAAGGGAGAC
    AGTGTGGGCC TCCGGCTGGC TGGTGGCAAT GACGTTGGGA TATTTGTGGC CGGCATTCAA
    GAGGGTACCT CCGCGGAGCA AGAAGGTCTT CAAGAAGGGG ACCAGATTCT GAAGGTGAAC
    ACACAGGATT TCAGAGGACT CGTTCGGGAG GATGCTGTTC TCTACCTGTT AGAAATCCCC
    AAAGGTGAAA CGGTGACCAT TTTAGCTCAG AGCCGAGCTG ATGTGTATAG AGACATACTG
    GCTTGTGGCA GAGGGGATTC ATTTTTTATA AGAAGCCACT TTGAATGTGA GAAGGAAACT
    CCACAGAGCC TGGCCTTCAC CAGAGGGGAG GTCTTCCGTG TGGTAGATAC ACTCTACGAT
    GGCAAACTGG GCCACTGGCT GGCCGTGAGG ATCGGGAACG AACTGGAGAA AGGCTTAATC
    CCCAACAAAA GCAGAGCGGA ACAAATGGCC AGCGTTCAGA ATGCCCAGAG AGACACCACC
    GGGGACAGGG CAGACTTCTG GAGGATGCGC GGCCAGAGAT CAGGTGTGAA GAAGAACCTA
    CGGAAGAGTC GGGAGGACCT GACTGCCGCC GTGACAGTCA GCACCAAGTT CCCGGCCTAC
    GAGAGGGTTT TGCTTCGAGA AGCTGGTTTC AAGAGACCTG TTGTCTTATT TGGCCCCATA
    GCAGATATAG CCCTGGAGAA GTTGGCAAAT GACTTGCCTG ACCTGTTCCA AACTGCTAAA
    ACAGAACCAA GAGACGCAGG ATCTGAGAAA TCCACCGGGG TGGTGCGGTT AAATACCGTG
    AGACAAATTA TTGAACAGGA TAAGCATGCA CTCCTGGATG TAACTCCTAA GGCTGTGGAC
    CTGTTGAACT ATACTCAGTG GTTCCCGATT GTGATCTTTT TCAACCCAGA CTCTAGGCAA
    GGTGTCAAAA CTATGAGACA GAGGTTGAAT CCCTCGTCCA ACAAAAGTTC TCGGAAGTTA
    TTTGATCAAG CCAACAAGCT TAAAAAAACT TGTGCACATC TTTTCACAGC TACAATCAAC
    CTCAATTCAG CCAACGACAG CTGGTTTGGC AGCTTGAAAG ACACGATTCA GCATCAGCAA
    GGAGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA TGGATGATGA TCCCGAAGAC
    CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA GTTGCGACAG CCGCCTCATC
    AGTGACTTTG AAGACACCGA CGGTGAAGGA GGTGCCTACA CTGACAATGA GCTGGATGAG
    CCAGCTGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT CGGAGCCGGT GCAGCATGAG
    GAGAGCATAA GGAAACCCAG CCCAGAGCCA CGAGCTCAGA TGAGGAGGGC TGCTAGCAGA
    GATCAACTTA GGGACAATAG CCCGCCCCCG ACATTCAAGC CAGAGCCGCC CAAGGCCAAA
    ACCCAAAACA GAGAAGAATC TTTTGACTTC TCCAGATCTT ATGAATACAA GTCAAACCCC
    TCAGCCGCTG CTGGTAACGA AATTCCAGGG GCATCTACCA AAGGTTGTCC TCCTCCTATT
    GCAGCGAAAC CCACTTTTGG ACGGCCTGTA CTGAAGCCCT CCACTCCCGT CCCTCCCCCA
    GAGAGTGAGG AGACGGGAGA GGGCAGTGAG GAGCAGCACA ATGCTCCCAA ATCTGTCCTG
    GGCAAAGTAA AAATATTTGA GAAGATGGAT CACAAGGCAA AGTTACAGAG AATGCAAGAG
    CTCCAAGAAG CACAGAATGC AAGGATTGAA ATTGCTCAGA AGCATCCTGA TATCTATGCA
    GTTCCAATCA AAACACACAA GCCGGACCCT GGCCCACCCC AGTACACAAG TTCCAGGCCC
    CCCGAGCCAC AGAAAGGCCC TGCCAGACTT TATCAGGATC CCAGAGGAAG CTATGGCAGT
    GATGCTGAGG AGGAGGAATA CCGGCAGCAG CTGTCAGAAC ACTCAAGGCA CGGTTATTAC
    GGCCAGCCTT CCCGATACCG GGACACGGAA TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45681
    Clone ID Related Accession (Same CDS sequence) XM_005210009.3
    Accession Version XM_005210009.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGTACA AGAAATATAT GACTATTCTG GAAGCAGCAG TGGGCATCAC TCCACTTAAC 
    AAAAGAGAAC TTCTACCTGA GAGCAGAAGA CACGTGAACC TTTGGCAGCA ACCTGGGGTG
    CGAAGTACTA CCTTGCTGTC ATCTCCTTGT GAGTGGGATT GGCATCCCAG CTGCACCTCT
    TGGCACAAGG GCTCCAGGAA ACACAGACAC GTGCCAACAG ACAGCCTGCT TTACCCGGCT
    CCAGGCATGG AAGAACTGAT ATGGGAACAG TACACTGTGA CCCTACAGAA GGATTCCAAG
    AGAGGATTTG GAATTGCAGT ATCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGGGAG
    ACATCCATCG TCATTTCTGA TGTGCTCCCG GGTGGGCCCG CCGATGGGCT GCTTCAAGAG
    AATGACAGGG TGGTCATGGT TAATGGCACC CCTATGGAAG ACGTGCTCCA TTCATTTGCG
    GTTCAGCAGC TGAGGAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCTGCA GGACAGTCCT CCTGTCCACG AAGATGACCG GGCTTTCGAG
    GTGATGGACG AGTTTGATGG CAGAAGCGCC CGCAGCGGCT ACAGCGAGAG GAGCCGGCCC
    AGCAGCCGCG GAGGGCGCAG CCGCAGCTGG GAGGAGAGCC CAGAGAGGGG CCGTCCCCAT
    GACCGGGTGC GCAGCCGGGA TCGAGGCCGG AGTCTGGAGC GGGGCCTGGA CCACGACGAC
    GACTACGGGC GAGCCCGGGA GCGCAGCCGT GGCCGCAGCC TCGAGCGGGG CCTAGACCAA
    GATGATTACA GGCGAGCCCG GGAGAGCAGC CGCGGCCGAA GCATTGACCC GGTCTACGAT
    CGCGCCTACA CCCCTGAGGG GGAGTACGGC CACAGGGCCC AGCCTGATGC CCGGTATGGG
    GCATCCCAGA GCCGCAGCCG TGAGCACCTC CACTCCCGCA GCCCCAGCCC GGAGCCCCGG
    GGGCGTCCGG ACTCGGACAA GCCTATCGGG GTCCTTATGA TGAAAAGCAA AGCAAATGAA
    GAATATGGTC TCCGGCTTGG GAGTCAGATC TTCATAAAGG AAATGACCAG AACCGGTCTA
    GCAACTAAAG ATGGCAACCT GCATGAAGGA GACATAATTC TCAAGATAAA CGGAACCGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAACTGATAG AAAAGTCAAG AGGGAAACTC
    CAGCTCGTGG TGCTGAGGGA CAGCAAACAG ACACTTATCA ACATCCCCTC ATTAAATGAC
    AGCGACTCAG AAATAGAAGA TATCTCAGAA ATAGAGTCCA ACCGATCGTT TTCTCCAGAG
    GAGAGGCGGC AGCAGTATTC TGATTATGAT TACCATTCCT CAAATGAAAA GCTAAAGGAG
    AGACCAAATT CAAGAGAGGA CATGCAGAGC AGATGGTCCA GGATGGGCGC CACACCCACT
    CCCTTTAAGT CCACTGGGGA TATGGCGGCT GCGGGCAGCG TGGAGCCCAG CAAGGAACCC
    AAATACCAAG AGGAAGCCCC AGTTCCTCCA CCAAAACCAG CCCCGAGAGC TGTTCTTCGT
    CCAAGTCCTG AAGATTTAGC AATATATGGC CCCAATACCA AAATGGTGAG GTTCAAGAAG
    GGAGACAGTG TGGGCCTCCG GCTGGCTGGT GGCAATGACG TTGGGATATT TGTGGCCGGC
    ATTCAAGAGG GTACCTCCGC GGAGCAAGAA GGTCTTCAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGACTCGTT CGGGAGGATG CTGTTCTCTA CCTGTTAGAA
    ATCCCCAAAG GTGAAACGGT GACCATTTTA GCTCAGAGCC GAGCTGATGT GTATAGAGAC
    ATACTGGCTT GTGGCAGAGG GGATTCATTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGTGTGGT AGATACACTC
    TACGATGGCA AACTGGGCCA CTGGCTGGCC GTGAGGATCG GGAACGAACT GGAGAAAGGC
    TTAATCCCCA ACAAAAGCAG AGCGGAACAA ATGGCCAGCG TTCAGAATGC CCAGAGAGAC
    ACCACCGGGG ACAGGGCAGA CTTCTGGAGG ATGCGCGGCC AGAGATCAGG TGTGAAGAAG
    AACCTACGGA AGAGTCGGGA GGACCTGACT GCCGCCGTGA CAGTCAGCAC CAAGTTCCCG
    GCCTACGAGA GGGTTTTGCT TCGAGAAGCT GGTTTCAAGA GACCTGTTGT CTTATTTGGC
    CCCATAGCAG ATATAGCCCT GGAGAAGTTG GCAAATGACT TGCCTGACCT GTTCCAAACT
    GCTAAAACAG AACCAAGAGA CGCAGGATCT GAGAAATCCA CCGGGGTGGT GCGGTTAAAT
    ACCGTGAGAC AAATTATTGA ACAGGATAAG CATGCACTCC TGGATGTAAC TCCTAAGGCT
    GTGGACCTGT TGAACTATAC TCAGTGGTTC CCGATTGTGA TCTTTTTCAA CCCAGACTCT
    AGGCAAGGTG TCAAAACTAT GAGACAGAGG TTGAATCCCT CGTCCAACAA AAGTTCTCGG
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACTTGTG CACATCTTTT CACAGCTACA
    ATCAACCTCA ATTCAGCCAA CGACAGCTGG TTTGGCAGCT TGAAAGACAC GATTCAGCAT
    CAGCAAGGAG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACCGACGGT GAAGGAGGTG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CATGAGGAGA GCATAAGGAA ACCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT
    AGCAGAGATC AACTTAGGGA CAATAGCCCG CCCCCGACAT TCAAGCCAGA GCCGCCCAAG
    GCCAAAACCC AAAACAGAGA AGAATCTTTT GACTTCTCCA GATCTTATGA ATACAAGTCA
    AACCCCTCAG CCGCTGCTGG TAACGAAATT CCAGGGGCAT CTACCAAAGG TTGTCCTCCT
    CCTATTGCAG CGAAACCCAC TTTTGGACGG CCTGTACTGA AGCCCTCCAC TCCCGTCCCT
    CCCCCAGAGA GTGAGGAGAC GGGAGAGGGC AGTGAGGAGC AGCACAATGC TCCCAAATCT
    GTCCTGGGCA AAGTAAAAAT ATTTGAGAAG ATGGATCACA AGGCAAAGTT ACAGAGAATG
    CAAGAGCTCC AAGAAGCACA GAATGCAAGG ATTGAAATTG CTCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC ACACAAGCCG GACCCTGGCC CACCCCAGTA CACAAGTTCC
    AGGCCCCCCG AGCCACAGAA AGGCCCTGCC AGACTTTATC AGGATCCCAG AGGAAGCTAT
    GGCAGTGATG CTGAGGAGGA GGAATACCGG CAGCAGCTGT CAGAACACTC AAGGCACGGT
    TATTACGGCC AGCCTTCCCG ATACCGGGAC ACGGAATTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210066.2
    CDS92..3793
    Translation

    Target ORF information:

    RefSeq Version XM_005210009.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGGTACA AGAAATATAT GACTATTCTG GAAGCAGCAG TGGGCATCAC TCCACTTAAC 
    AAAAGAGAAC TTCTACCTGA GAGCAGAAGA CACGTGAACC TTTGGCAGCA ACCTGGGGTG
    CGAAGTACTA CCTTGCTGTC ATCTCCTTGT GAGTGGGATT GGCATCCCAG CTGCACCTCT
    TGGCACAAGG GCTCCAGGAA ACACAGACAC GTGCCAACAG ACAGCCTGCT TTACCCGGCT
    CCAGGCATGG AAGAACTGAT ATGGGAACAG TACACTGTGA CCCTACAGAA GGATTCCAAG
    AGAGGATTTG GAATTGCAGT ATCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGGGAG
    ACATCCATCG TCATTTCTGA TGTGCTCCCG GGTGGGCCCG CCGATGGGCT GCTTCAAGAG
    AATGACAGGG TGGTCATGGT TAATGGCACC CCTATGGAAG ACGTGCTCCA TTCATTTGCG
    GTTCAGCAGC TGAGGAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCTGCA GGACAGTCCT CCTGTCCACG AAGATGACCG GGCTTTCGAG
    GTGATGGACG AGTTTGATGG CAGAAGCGCC CGCAGCGGCT ACAGCGAGAG GAGCCGGCCC
    AGCAGCCGCG GAGGGCGCAG CCGCAGCTGG GAGGAGAGCC CAGAGAGGGG CCGTCCCCAT
    GACCGGGTGC GCAGCCGGGA TCGAGGCCGG AGTCTGGAGC GGGGCCTGGA CCACGACGAC
    GACTACGGGC GAGCCCGGGA GCGCAGCCGT GGCCGCAGCC TCGAGCGGGG CCTAGACCAA
    GATGATTACA GGCGAGCCCG GGAGAGCAGC CGCGGCCGAA GCATTGACCC GGTCTACGAT
    CGCGCCTACA CCCCTGAGGG GGAGTACGGC CACAGGGCCC AGCCTGATGC CCGGTATGGG
    GCATCCCAGA GCCGCAGCCG TGAGCACCTC CACTCCCGCA GCCCCAGCCC GGAGCCCCGG
    GGGCGTCCGG ACTCGGACAA GCCTATCGGG GTCCTTATGA TGAAAAGCAA AGCAAATGAA
    GAATATGGTC TCCGGCTTGG GAGTCAGATC TTCATAAAGG AAATGACCAG AACCGGTCTA
    GCAACTAAAG ATGGCAACCT GCATGAAGGA GACATAATTC TCAAGATAAA CGGAACCGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAACTGATAG AAAAGTCAAG AGGGAAACTC
    CAGCTCGTGG TGCTGAGGGA CAGCAAACAG ACACTTATCA ACATCCCCTC ATTAAATGAC
    AGCGACTCAG AAATAGAAGA TATCTCAGAA ATAGAGTCCA ACCGATCGTT TTCTCCAGAG
    GAGAGGCGGC AGCAGTATTC TGATTATGAT TACCATTCCT CAAATGAAAA GCTAAAGGAG
    AGACCAAATT CAAGAGAGGA CATGCAGAGC AGATGGTCCA GGATGGGCGC CACACCCACT
    CCCTTTAAGT CCACTGGGGA TATGGCGGCT GCGGGCAGCG TGGAGCCCAG CAAGGAACCC
    AAATACCAAG AGGAAGCCCC AGTTCCTCCA CCAAAACCAG CCCCGAGAGC TGTTCTTCGT
    CCAAGTCCTG AAGATTTAGC AATATATGGC CCCAATACCA AAATGGTGAG GTTCAAGAAG
    GGAGACAGTG TGGGCCTCCG GCTGGCTGGT GGCAATGACG TTGGGATATT TGTGGCCGGC
    ATTCAAGAGG GTACCTCCGC GGAGCAAGAA GGTCTTCAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGACTCGTT CGGGAGGATG CTGTTCTCTA CCTGTTAGAA
    ATCCCCAAAG GTGAAACGGT GACCATTTTA GCTCAGAGCC GAGCTGATGT GTATAGAGAC
    ATACTGGCTT GTGGCAGAGG GGATTCATTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGTGTGGT AGATACACTC
    TACGATGGCA AACTGGGCCA CTGGCTGGCC GTGAGGATCG GGAACGAACT GGAGAAAGGC
    TTAATCCCCA ACAAAAGCAG AGCGGAACAA ATGGCCAGCG TTCAGAATGC CCAGAGAGAC
    ACCACCGGGG ACAGGGCAGA CTTCTGGAGG ATGCGCGGCC AGAGATCAGG TGTGAAGAAG
    AACCTACGGA AGAGTCGGGA GGACCTGACT GCCGCCGTGA CAGTCAGCAC CAAGTTCCCG
    GCCTACGAGA GGGTTTTGCT TCGAGAAGCT GGTTTCAAGA GACCTGTTGT CTTATTTGGC
    CCCATAGCAG ATATAGCCCT GGAGAAGTTG GCAAATGACT TGCCTGACCT GTTCCAAACT
    GCTAAAACAG AACCAAGAGA CGCAGGATCT GAGAAATCCA CCGGGGTGGT GCGGTTAAAT
    ACCGTGAGAC AAATTATTGA ACAGGATAAG CATGCACTCC TGGATGTAAC TCCTAAGGCT
    GTGGACCTGT TGAACTATAC TCAGTGGTTC CCGATTGTGA TCTTTTTCAA CCCAGACTCT
    AGGCAAGGTG TCAAAACTAT GAGACAGAGG TTGAATCCCT CGTCCAACAA AAGTTCTCGG
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACTTGTG CACATCTTTT CACAGCTACA
    ATCAACCTCA ATTCAGCCAA CGACAGCTGG TTTGGCAGCT TGAAAGACAC GATTCAGCAT
    CAGCAAGGAG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACCGACGGT GAAGGAGGTG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CATGAGGAGA GCATAAGGAA ACCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT
    AGCAGAGATC AACTTAGGGA CAATAGCCCG CCCCCGACAT TCAAGCCAGA GCCGCCCAAG
    GCCAAAACCC AAAACAGAGA AGAATCTTTT GACTTCTCCA GATCTTATGA ATACAAGTCA
    AACCCCTCAG CCGCTGCTGG TAACGAAATT CCAGGGGCAT CTACCAAAGG TTGTCCTCCT
    CCTATTGCAG CGAAACCCAC TTTTGGACGG CCTGTACTGA AGCCCTCCAC TCCCGTCCCT
    CCCCCAGAGA GTGAGGAGAC GGGAGAGGGC AGTGAGGAGC AGCACAATGC TCCCAAATCT
    GTCCTGGGCA AAGTAAAAAT ATTTGAGAAG ATGGATCACA AGGCAAAGTT ACAGAGAATG
    CAAGAGCTCC AAGAAGCACA GAATGCAAGG ATTGAAATTG CTCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC ACACAAGCCG GACCCTGGCC CACCCCAGTA CACAAGTTCC
    AGGCCCCCCG AGCCACAGAA AGGCCCTGCC AGACTTTATC AGGATCCCAG AGGAAGCTAT
    GGCAGTGATG CTGAGGAGGA GGAATACCGG CAGCAGCTGT CAGAACACTC AAGGCACGGT
    TATTACGGCC AGCCTTCCCG ATACCGGGAC ACGGAATTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45682
    Clone ID Related Accession (Same CDS sequence) XM_010807868.2
    Accession Version XM_010807868.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGTACA AGAAATATAT GACTATTCTG GAAGCAGCAG TGGGCATCAC TCCACTTAAC 
    AAAAGAGAAC TTCTACCTGA GAGCAGAAGA CACGTGAACC TTTGGCAGCA ACCTGGGGTG
    CGAAGTACTA CCTTGCTGTC ATCTCCTTGT GAGTGGGATT GGCATCCCAG CTGCACCTCT
    TGGCACAAGG GCTCCAGGAA ACACAGACAC GTGCCAACAG ACAGCCTGCT TTACCCGGCT
    CCAGGCATGG AAGAACTGAT ATGGGAACAG TACACTGTGA CCCTACAGAA GGATTCCAAG
    AGAGGATTTG GAATTGCAGT ATCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGGGAG
    ACATCCATCG TCATTTCTGA TGTGCTCCCG GGTGGGCCCG CCGATGGGCT GCTTCAAGAG
    AATGACAGGG TGGTCATGGT TAATGGCACC CCTATGGAAG ACGTGCTCCA TTCATTTGCG
    GTTCAGCAGC TGAGGAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCTGCA GGACAGTCCT CCTGTCCACG AAGATGACCG GGCTTTCGAG
    GTGATGGACG AGTTTGATGG CAGAAGCGCC CGCAGCGGCT ACAGCGAGAG GAGCCGGCCC
    AGCAGCCGCG GAGGGCGCAG CCGCAGCTGG GAGGAGAGCC CAGAGAGGGG CCGTCCCCAT
    GACCGGGTGC GCAGCCGGGA TCGAGGCCGG AGTCTGGAGC GGGGCCTGGA CCACGACGAC
    GACTACGGGC GAGCCCGGGA GCGCAGCCGT GGCCGCAGCC TCGAGCGGGG CCTAGACCAA
    GATGATTACA GGCGAGCCCG GGAGAGCAGC CGCGGCCGAA GCATTGACCC GGTCTACGAT
    CGCGCCTACA CCCCTGAGGG GGAGTACGGC CACAGGGCCC AGCCTGATGC CCGGTATGGG
    GCATCCCAGA GCCGCAGCCG TGAGCACCTC CACTCCCGCA GCCCCAGCCC GGAGCCCCGG
    GGGCGTCCGG ACTCGGACAA GCCTATCGGG GTCCTTATGA TGAAAAGCAA AGCAAATGAA
    GAATATGGTC TCCGGCTTGG GAGTCAGATC TTCATAAAGG AAATGACCAG AACCGGTCTA
    GCAACTAAAG ATGGCAACCT GCATGAAGGA GACATAATTC TCAAGATAAA CGGAACCGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAACTGATAG AAAAGTCAAG AGGGAAACTC
    CAGCTCGTGG TGCTGAGGGA CAGCAAACAG ACACTTATCA ACATCCCCTC ATTAAATGAC
    AGCGACTCAG AAATAGAAGA TATCTCAGAA ATAGAGTCCA ACCGATCGTT TTCTCCAGAG
    GAGAGGCGGC AGCAGTATTC TGATTATGAT TACCATTCCT CAAATGAAAA GCTAAAGGAG
    AGACCAAATT CAAGAGAGGA CATGCAGAGC AGATGGTCCA GGATGGGCGC CACACCCACT
    CCCTTTAAGT CCACTGGGGA TATGGCGGCT GCGGGCAGCG TGGAGCCCAG CAAGGAACCC
    AAATACCAAG AGGAAGCCCC AGTTCCTCCA CCAAAACCAG CCCCGAGAGC TGTTCTTCGT
    CCAAGTCCTG AAGATTTAGC AATATATGGC CCCAATACCA AAATGGTGAG GTTCAAGAAG
    GGAGACAGTG TGGGCCTCCG GCTGGCTGGT GGCAATGACG TTGGGATATT TGTGGCCGGC
    ATTCAAGAGG GTACCTCCGC GGAGCAAGAA GGTCTTCAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGACTCGTT CGGGAGGATG CTGTTCTCTA CCTGTTAGAA
    ATCCCCAAAG GTGAAACGGT GACCATTTTA GCTCAGAGCC GAGCTGATGT GTATAGAGAC
    ATACTGGCTT GTGGCAGAGG GGATTCATTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGTGTGGT AGATACACTC
    TACGATGGCA AACTGGGCCA CTGGCTGGCC GTGAGGATCG GGAACGAACT GGAGAAAGGC
    TTAATCCCCA ACAAAAGCAG AGCGGAACAA ATGGCCAGCG TTCAGAATGC CCAGAGAGAC
    ACCACCGGGG ACAGGGCAGA CTTCTGGAGG ATGCGCGGCC AGAGATCAGG TGTGAAGAAG
    AACCTACGGA AGAGTCGGGA GGACCTGACT GCCGCCGTGA CAGTCAGCAC CAAGTTCCCG
    GCCTACGAGA GGGTTTTGCT TCGAGAAGCT GGTTTCAAGA GACCTGTTGT CTTATTTGGC
    CCCATAGCAG ATATAGCCCT GGAGAAGTTG GCAAATGACT TGCCTGACCT GTTCCAAACT
    GCTAAAACAG AACCAAGAGA CGCAGGATCT GAGAAATCCA CCGGGGTGGT GCGGTTAAAT
    ACCGTGAGAC AAATTATTGA ACAGGATAAG CATGCACTCC TGGATGTAAC TCCTAAGGCT
    GTGGACCTGT TGAACTATAC TCAGTGGTTC CCGATTGTGA TCTTTTTCAA CCCAGACTCT
    AGGCAAGGTG TCAAAACTAT GAGACAGAGG TTGAATCCCT CGTCCAACAA AAGTTCTCGG
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACTTGTG CACATCTTTT CACAGCTACA
    ATCAACCTCA ATTCAGCCAA CGACAGCTGG TTTGGCAGCT TGAAAGACAC GATTCAGCAT
    CAGCAAGGAG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACCGACGGT GAAGGAGGTG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CATGAGGAGG CCAAAACCCA AAACAGAGAA GAATCTTTTG ACTTCTCCAG ATCTTATGAA
    TACAAGTCAA ACCCCTCAGC CGCTGCTGGT AACGAAATTC CAGGGGCATC TACCAAAGGT
    TGTCCTCCTC CTATTGCAGC GAAACCCACT TTTGGACGGC CTGTACTGAA GCCCTCCACT
    CCCGTCCCTC CCCCAGAGAG TGAGGAGACG GGAGAGGGCA GTGAGGAGCA GCACAATGCT
    CCCAAATCTG TCCTGGGCAA AGTAAAAATA TTTGAGAAGA TGGATCACAA GGCAAAGTTA
    CAGAGAATGC AAGAGCTCCA AGAAGCACAG AATGCAAGGA TTGAAATTGC TCAGAAGCAT
    CCTGATATCT ATGCAGTTCC AATCAAAACA CACAAGCCGG ACCCTGGCCC ACCCCAGTAC
    ACAAGTTCCA GGCCCCCCGA GCCACAGAAA GGCCCTGCCA GACTTTATCA GGATCCCAGA
    GGAAGCTATG GCAGTGATGC TGAGGAGGAG GAATACCGGC AGCAGCTGTC AGAACACTCA
    AGGCACGGTT ATTACGGCCA GCCTTCCCGA TACCGGGACA CGGAATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806170.1
    CDS94..3684
    Translation

    Target ORF information:

    RefSeq Version XM_010807868.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807868.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGTACA AGAAATATAT GACTATTCTG GAAGCAGCAG TGGGCATCAC TCCACTTAAC 
    AAAAGAGAAC TTCTACCTGA GAGCAGAAGA CACGTGAACC TTTGGCAGCA ACCTGGGGTG
    CGAAGTACTA CCTTGCTGTC ATCTCCTTGT GAGTGGGATT GGCATCCCAG CTGCACCTCT
    TGGCACAAGG GCTCCAGGAA ACACAGACAC GTGCCAACAG ACAGCCTGCT TTACCCGGCT
    CCAGGCATGG AAGAACTGAT ATGGGAACAG TACACTGTGA CCCTACAGAA GGATTCCAAG
    AGAGGATTTG GAATTGCAGT ATCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGGGAG
    ACATCCATCG TCATTTCTGA TGTGCTCCCG GGTGGGCCCG CCGATGGGCT GCTTCAAGAG
    AATGACAGGG TGGTCATGGT TAATGGCACC CCTATGGAAG ACGTGCTCCA TTCATTTGCG
    GTTCAGCAGC TGAGGAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCTGCA GGACAGTCCT CCTGTCCACG AAGATGACCG GGCTTTCGAG
    GTGATGGACG AGTTTGATGG CAGAAGCGCC CGCAGCGGCT ACAGCGAGAG GAGCCGGCCC
    AGCAGCCGCG GAGGGCGCAG CCGCAGCTGG GAGGAGAGCC CAGAGAGGGG CCGTCCCCAT
    GACCGGGTGC GCAGCCGGGA TCGAGGCCGG AGTCTGGAGC GGGGCCTGGA CCACGACGAC
    GACTACGGGC GAGCCCGGGA GCGCAGCCGT GGCCGCAGCC TCGAGCGGGG CCTAGACCAA
    GATGATTACA GGCGAGCCCG GGAGAGCAGC CGCGGCCGAA GCATTGACCC GGTCTACGAT
    CGCGCCTACA CCCCTGAGGG GGAGTACGGC CACAGGGCCC AGCCTGATGC CCGGTATGGG
    GCATCCCAGA GCCGCAGCCG TGAGCACCTC CACTCCCGCA GCCCCAGCCC GGAGCCCCGG
    GGGCGTCCGG ACTCGGACAA GCCTATCGGG GTCCTTATGA TGAAAAGCAA AGCAAATGAA
    GAATATGGTC TCCGGCTTGG GAGTCAGATC TTCATAAAGG AAATGACCAG AACCGGTCTA
    GCAACTAAAG ATGGCAACCT GCATGAAGGA GACATAATTC TCAAGATAAA CGGAACCGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAACTGATAG AAAAGTCAAG AGGGAAACTC
    CAGCTCGTGG TGCTGAGGGA CAGCAAACAG ACACTTATCA ACATCCCCTC ATTAAATGAC
    AGCGACTCAG AAATAGAAGA TATCTCAGAA ATAGAGTCCA ACCGATCGTT TTCTCCAGAG
    GAGAGGCGGC AGCAGTATTC TGATTATGAT TACCATTCCT CAAATGAAAA GCTAAAGGAG
    AGACCAAATT CAAGAGAGGA CATGCAGAGC AGATGGTCCA GGATGGGCGC CACACCCACT
    CCCTTTAAGT CCACTGGGGA TATGGCGGCT GCGGGCAGCG TGGAGCCCAG CAAGGAACCC
    AAATACCAAG AGGAAGCCCC AGTTCCTCCA CCAAAACCAG CCCCGAGAGC TGTTCTTCGT
    CCAAGTCCTG AAGATTTAGC AATATATGGC CCCAATACCA AAATGGTGAG GTTCAAGAAG
    GGAGACAGTG TGGGCCTCCG GCTGGCTGGT GGCAATGACG TTGGGATATT TGTGGCCGGC
    ATTCAAGAGG GTACCTCCGC GGAGCAAGAA GGTCTTCAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGACTCGTT CGGGAGGATG CTGTTCTCTA CCTGTTAGAA
    ATCCCCAAAG GTGAAACGGT GACCATTTTA GCTCAGAGCC GAGCTGATGT GTATAGAGAC
    ATACTGGCTT GTGGCAGAGG GGATTCATTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGTGTGGT AGATACACTC
    TACGATGGCA AACTGGGCCA CTGGCTGGCC GTGAGGATCG GGAACGAACT GGAGAAAGGC
    TTAATCCCCA ACAAAAGCAG AGCGGAACAA ATGGCCAGCG TTCAGAATGC CCAGAGAGAC
    ACCACCGGGG ACAGGGCAGA CTTCTGGAGG ATGCGCGGCC AGAGATCAGG TGTGAAGAAG
    AACCTACGGA AGAGTCGGGA GGACCTGACT GCCGCCGTGA CAGTCAGCAC CAAGTTCCCG
    GCCTACGAGA GGGTTTTGCT TCGAGAAGCT GGTTTCAAGA GACCTGTTGT CTTATTTGGC
    CCCATAGCAG ATATAGCCCT GGAGAAGTTG GCAAATGACT TGCCTGACCT GTTCCAAACT
    GCTAAAACAG AACCAAGAGA CGCAGGATCT GAGAAATCCA CCGGGGTGGT GCGGTTAAAT
    ACCGTGAGAC AAATTATTGA ACAGGATAAG CATGCACTCC TGGATGTAAC TCCTAAGGCT
    GTGGACCTGT TGAACTATAC TCAGTGGTTC CCGATTGTGA TCTTTTTCAA CCCAGACTCT
    AGGCAAGGTG TCAAAACTAT GAGACAGAGG TTGAATCCCT CGTCCAACAA AAGTTCTCGG
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACTTGTG CACATCTTTT CACAGCTACA
    ATCAACCTCA ATTCAGCCAA CGACAGCTGG TTTGGCAGCT TGAAAGACAC GATTCAGCAT
    CAGCAAGGAG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACCGACGGT GAAGGAGGTG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CATGAGGAGG CCAAAACCCA AAACAGAGAA GAATCTTTTG ACTTCTCCAG ATCTTATGAA
    TACAAGTCAA ACCCCTCAGC CGCTGCTGGT AACGAAATTC CAGGGGCATC TACCAAAGGT
    TGTCCTCCTC CTATTGCAGC GAAACCCACT TTTGGACGGC CTGTACTGAA GCCCTCCACT
    CCCGTCCCTC CCCCAGAGAG TGAGGAGACG GGAGAGGGCA GTGAGGAGCA GCACAATGCT
    CCCAAATCTG TCCTGGGCAA AGTAAAAATA TTTGAGAAGA TGGATCACAA GGCAAAGTTA
    CAGAGAATGC AAGAGCTCCA AGAAGCACAG AATGCAAGGA TTGAAATTGC TCAGAAGCAT
    CCTGATATCT ATGCAGTTCC AATCAAAACA CACAAGCCGG ACCCTGGCCC ACCCCAGTAC
    ACAAGTTCCA GGCCCCCCGA GCCACAGAAA GGCCCTGCCA GACTTTATCA GGATCCCAGA
    GGAAGCTATG GCAGTGATGC TGAGGAGGAG GAATACCGGC AGCAGCTGTC AGAACACTCA
    AGGCACGGTT ATTACGGCCA GCCTTCCCGA TACCGGGACA CGGAATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45683
    Clone ID Related Accession (Same CDS sequence) XM_010807870.2
    Accession Version XM_010807870.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tight junction protein ZO-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTACA AGAAATATAT GACTATTCTG GAAGCAGCAG TGGGCATCAC TCCACTTAAC 
    AAAAGAGAAC TTCTACCTGA GAGCAGAAGA CACGTGAACC TTTGGCAGCA ACCTGGGGTG
    CGAAGTACTA CCTTGCTGTC ATCTCCTTGT GAGTGGGATT GGCATCCCAG CTGCACCTCT
    TGGCACAAGG GCTCCAGGAA ACACAGACAC GTGCCAACAG ACAGCCTGCT TTACCCGGCT
    CCAGGCATGG AAGAACTGAT ATGGGAACAG TACACTGTGA CCCTACAGAA GGATTCCAAG
    AGAGGATTTG GAATTGCAGT ATCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGGGAG
    ACATCCATCG TCATTTCTGA TGTGCTCCCG GGTGGGCCCG CCGATGGGCT GCTTCAAGAG
    AATGACAGGG TGGTCATGGT TAATGGCACC CCTATGGAAG ACGTGCTCCA TTCATTTGCG
    GTTCAGCAGC TGAGGAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCTGCA GGACAGTCCT CCTGTCCACG AAGATGACCG GGCTTTCGAG
    GTGATGGACG AGTTTGATGG CAGAAGCGCC CGCAGCGGCT ACAGCGAGAG GAGCCGGCCC
    AGCAGCCGCG GAGGGCGCAG CCGCAGCTGG GAGGAGAGCC CAGAGAGGGG CCGTCCCCAT
    GACCGGGTGC GCAGCCGGGA TCGAGGCCGG AGTCTGGAGC GGGGCCTGGA CCACGACGAC
    GACTACGGGC GAGCCCGGGA GCGCAGCCGT GGCCGCAGCC TCGAGCGGGG CCTAGACCAA
    GATGATTACA GGCGAGCCCG GGAGAGCAGC CGCGGCCGAA GCATTGACCC GGTCTACGAT
    CGCGCCTACA CCCCTGAGGG GGAGTACGGC CACAGGGCCC AGCCTGATGC CCGGTATGGG
    GCATCCCAGA GCCGCAGCCG TGAGCACCTC CACTCCCGCA GCCCCAGCCC GGAGCCCCGG
    GGGCGTCCGG ACTCGGACAA GCCTATCGGG GTCCTTATGA TGAAAAGCAA AGCAAATGAA
    GAATATGGTC TCCGGCTTGG GAGTCAGATC TTCATAAAGG AAATGACCAG AACCGGTCTA
    GCAACTAAAG ATGGCAACCT GCATGAAGGA GACATAATTC TCAAGATAAA CGGAACCGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAACTGATAG AAAAGTCAAG AGGGAAACTC
    CAGCTCGTGG TGCTGAGGGA CAGCAAACAG ACACTTATCA ACATCCCCTC ATTAAATGAC
    AGCGACTCAG AAATAGAAGA TATCTCAGAA ATAGAGTCCA ACCGATCGTT TTCTCCAGAG
    GAGAGGCGGC AGCAGTATTC TGATTATGAT TACCATTCCT CAAATGAAAA GCTAAAGGAG
    AGACCAAATT CAAGAGAGGA CATGCAGAGC AGATGGTCCA GGATGGGCGC CACACCCACT
    CCCTTTAAGT CCACTGGGGA TATGGCGGCT GCGGGCAGCG TGGAGCCCAG CAAGGAACCC
    AAATACCAAG AGGAAGCCCC AGTTCCTCCA CCAAAACCAG CCCCGAGAGC TGTTCTTCGT
    CCAAGTCCTG AAGATTTAGC AATATATGGC CCCAATACCA AAATGGTGAG GTTCAAGAAG
    GGAGACAGTG TGGGCCTCCG GCTGGCTGGT GGCAATGACG TTGGGATATT TGTGGCCGGC
    ATTCAAGAGG GTACCTCCGC GGAGCAAGAA GGTCTTCAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGACTCGTT CGGGAGGATG CTGTTCTCTA CCTGTTAGAA
    ATCCCCAAAG GTGAAACGGT GACCATTTTA GCTCAGAGCC GAGCTGATGT GTATAGAGAC
    ATACTGGCTT GTGGCAGAGG GGATTCATTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGTGTGGT AGATACACTC
    TACGATGGCA AACTGGGCCA CTGGCTGGCC GTGAGGATCG GGAACGAACT GGAGAAAGGC
    TTAATCCCCA ACAAAAGCAG AGCGGAACAA ATGGCCAGCG TTCAGAATGC CCAGAGAGAC
    ACCACCGGGG ACAGGGCAGA CTTCTGGAGG ATGCGCGGCC AGAGATCAGG TGTGAAGAAG
    AACCTACGGA AGAGTCGGGA GGACCTGACT GCCGCCGTGA CAGTCAGCAC CAAGTTCCCG
    GCCTACGAGA GGGTTTTGCT TCGAGAAGCT GGTTTCAAGA GACCTGTTGT CTTATTTGGC
    CCCATAGCAG ATATAGCCCT GGAGAAGTTG GCAAATGACT TGCCTGACCT GTTCCAAACT
    GCTAAAACAG AACCAAGAGA CGCAGGATCT GAGAAATCCA CCGGGGTGGT GCGGTTAAAT
    ACCGTGAGAC AAATTATTGA ACAGGATAAG CATGCACTCC TGGATGTAAC TCCTAAGGCT
    GTGGACCTGT TGAACTATAC TCAGTGGTTC CCGATTGTGA TCTTTTTCAA CCCAGACTCT
    AGGCAAGGTG TCAAAACTAT GAGACAGAGG TTGAATCCCT CGTCCAACAA AAGTTCTCGG
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACTTGTG CACATCTTTT CACAGCTACA
    ATCAACCTCA ATTCAGCCAA CGACAGCTGG TTTGGCAGCT TGAAAGACAC GATTCAGCAT
    CAGCAAGGAG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACCGACGGT GAAGGAGGTG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CATGAGGAGA TTGAAATTGC TCAGAAGCAT CCTGATATCT ATGCAGTTCC AATCAAAACA
    CACAAGCCGG ACCCTGGCCC ACCCCAGTAC ACAAGTTCCA GGCCCCCCGA GCCACAGAAA
    GGCCCTGCCA GACTTTATCA GGATCCCAGA GGAAGCTATG GCAGTGATGC TGAGGAGGAG
    GAATACCGGC AGCAGCTGTC AGAACACTCA AGGCACGGTT ATTACGGCCA GCCTTCCCGA
    TACCGGGACA CGGAATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806172.1
    CDS97..3357
    Translation

    Target ORF information:

    RefSeq Version XM_010807870.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807870.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTACA AGAAATATAT GACTATTCTG GAAGCAGCAG TGGGCATCAC TCCACTTAAC 
    AAAAGAGAAC TTCTACCTGA GAGCAGAAGA CACGTGAACC TTTGGCAGCA ACCTGGGGTG
    CGAAGTACTA CCTTGCTGTC ATCTCCTTGT GAGTGGGATT GGCATCCCAG CTGCACCTCT
    TGGCACAAGG GCTCCAGGAA ACACAGACAC GTGCCAACAG ACAGCCTGCT TTACCCGGCT
    CCAGGCATGG AAGAACTGAT ATGGGAACAG TACACTGTGA CCCTACAGAA GGATTCCAAG
    AGAGGATTTG GAATTGCAGT ATCCGGAGGC AGAGACAACC CCCACTTTGA AAATGGGGAG
    ACATCCATCG TCATTTCTGA TGTGCTCCCG GGTGGGCCCG CCGATGGGCT GCTTCAAGAG
    AATGACAGGG TGGTCATGGT TAATGGCACC CCTATGGAAG ACGTGCTCCA TTCATTTGCG
    GTTCAGCAGC TGAGGAAAAG TGGGAAGATC GCTGCCATTG TGGTCAAGAG GCCCCGGAAG
    GTCCAGCTGG CCCCGCTGCA GGACAGTCCT CCTGTCCACG AAGATGACCG GGCTTTCGAG
    GTGATGGACG AGTTTGATGG CAGAAGCGCC CGCAGCGGCT ACAGCGAGAG GAGCCGGCCC
    AGCAGCCGCG GAGGGCGCAG CCGCAGCTGG GAGGAGAGCC CAGAGAGGGG CCGTCCCCAT
    GACCGGGTGC GCAGCCGGGA TCGAGGCCGG AGTCTGGAGC GGGGCCTGGA CCACGACGAC
    GACTACGGGC GAGCCCGGGA GCGCAGCCGT GGCCGCAGCC TCGAGCGGGG CCTAGACCAA
    GATGATTACA GGCGAGCCCG GGAGAGCAGC CGCGGCCGAA GCATTGACCC GGTCTACGAT
    CGCGCCTACA CCCCTGAGGG GGAGTACGGC CACAGGGCCC AGCCTGATGC CCGGTATGGG
    GCATCCCAGA GCCGCAGCCG TGAGCACCTC CACTCCCGCA GCCCCAGCCC GGAGCCCCGG
    GGGCGTCCGG ACTCGGACAA GCCTATCGGG GTCCTTATGA TGAAAAGCAA AGCAAATGAA
    GAATATGGTC TCCGGCTTGG GAGTCAGATC TTCATAAAGG AAATGACCAG AACCGGTCTA
    GCAACTAAAG ATGGCAACCT GCATGAAGGA GACATAATTC TCAAGATAAA CGGAACCGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAACTGATAG AAAAGTCAAG AGGGAAACTC
    CAGCTCGTGG TGCTGAGGGA CAGCAAACAG ACACTTATCA ACATCCCCTC ATTAAATGAC
    AGCGACTCAG AAATAGAAGA TATCTCAGAA ATAGAGTCCA ACCGATCGTT TTCTCCAGAG
    GAGAGGCGGC AGCAGTATTC TGATTATGAT TACCATTCCT CAAATGAAAA GCTAAAGGAG
    AGACCAAATT CAAGAGAGGA CATGCAGAGC AGATGGTCCA GGATGGGCGC CACACCCACT
    CCCTTTAAGT CCACTGGGGA TATGGCGGCT GCGGGCAGCG TGGAGCCCAG CAAGGAACCC
    AAATACCAAG AGGAAGCCCC AGTTCCTCCA CCAAAACCAG CCCCGAGAGC TGTTCTTCGT
    CCAAGTCCTG AAGATTTAGC AATATATGGC CCCAATACCA AAATGGTGAG GTTCAAGAAG
    GGAGACAGTG TGGGCCTCCG GCTGGCTGGT GGCAATGACG TTGGGATATT TGTGGCCGGC
    ATTCAAGAGG GTACCTCCGC GGAGCAAGAA GGTCTTCAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGACTCGTT CGGGAGGATG CTGTTCTCTA CCTGTTAGAA
    ATCCCCAAAG GTGAAACGGT GACCATTTTA GCTCAGAGCC GAGCTGATGT GTATAGAGAC
    ATACTGGCTT GTGGCAGAGG GGATTCATTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGTGTGGT AGATACACTC
    TACGATGGCA AACTGGGCCA CTGGCTGGCC GTGAGGATCG GGAACGAACT GGAGAAAGGC
    TTAATCCCCA ACAAAAGCAG AGCGGAACAA ATGGCCAGCG TTCAGAATGC CCAGAGAGAC
    ACCACCGGGG ACAGGGCAGA CTTCTGGAGG ATGCGCGGCC AGAGATCAGG TGTGAAGAAG
    AACCTACGGA AGAGTCGGGA GGACCTGACT GCCGCCGTGA CAGTCAGCAC CAAGTTCCCG
    GCCTACGAGA GGGTTTTGCT TCGAGAAGCT GGTTTCAAGA GACCTGTTGT CTTATTTGGC
    CCCATAGCAG ATATAGCCCT GGAGAAGTTG GCAAATGACT TGCCTGACCT GTTCCAAACT
    GCTAAAACAG AACCAAGAGA CGCAGGATCT GAGAAATCCA CCGGGGTGGT GCGGTTAAAT
    ACCGTGAGAC AAATTATTGA ACAGGATAAG CATGCACTCC TGGATGTAAC TCCTAAGGCT
    GTGGACCTGT TGAACTATAC TCAGTGGTTC CCGATTGTGA TCTTTTTCAA CCCAGACTCT
    AGGCAAGGTG TCAAAACTAT GAGACAGAGG TTGAATCCCT CGTCCAACAA AAGTTCTCGG
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACTTGTG CACATCTTTT CACAGCTACA
    ATCAACCTCA ATTCAGCCAA CGACAGCTGG TTTGGCAGCT TGAAAGACAC GATTCAGCAT
    CAGCAAGGAG AAGCAGTCTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGATCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACCGACGGT GAAGGAGGTG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CATGAGGAGA TTGAAATTGC TCAGAAGCAT CCTGATATCT ATGCAGTTCC AATCAAAACA
    CACAAGCCGG ACCCTGGCCC ACCCCAGTAC ACAAGTTCCA GGCCCCCCGA GCCACAGAAA
    GGCCCTGCCA GACTTTATCA GGATCCCAGA GGAAGCTATG GCAGTGATGC TGAGGAGGAG
    GAATACCGGC AGCAGCTGTC AGAACACTCA AGGCACGGTT ATTACGGCCA GCCTTCCCGA
    TACCGGGACA CGGAATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.