SLC12A3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A3
Entrez Gene ID	404171
Full Name	solute carrier family 12 member 3
General protein information	Preferred Names solute carrier family 12 member 3 Names solute carrier family 12 member 3 sodium chloride cotransporter solute carrier family 12 (sodium/chloride transporters), member 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206178.1	NP_001193107.1	solute carrier family 12 member 3
XM_005218464.3	XP_005218521.1	solute carrier family 12 member 3 isoform X1
XM_005218465.4	XP_005218522.1	solute carrier family 12 member 3 isoform X2
XM_005218464.4	XP_005218521.1	solute carrier family 12 member 3 isoform X1
XM_015475706.2	XP_015331192.1	solute carrier family 12 member 3 isoform X3
XM_005218465.3	XP_005218522.1	solute carrier family 12 member 3 isoform X2
XM_015475706.1	XP_015331192.1	solute carrier family 12 member 3 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc12a3	NP_062288.2
Rattus norvegicus (Norway rat)	Slc12a3	NP_062218.3
Xenopus tropicalis (tropical clawed frog)	slc12a3	XP_002937217.2
Gallus gallus (chicken)	SLC12A3	XP_414059.4
	SLC12A3	XP_001093467.2
Canis lupus familiaris (dog)	SLC12A3	XP_005617594.1
Bos taurus (cattle)	SLC12A3	NP_001193107.1
Danio rerio (zebrafish)	slc12a3	NP_001038545.1
Pan troglodytes (chimpanzee)	SLC12A3	XP_510983.2
Homo sapiens (human)	SLC12A3	NP_000330.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Rare independent mutations in renal salt handling genes contribute to blood pressure variation.
Ji W, Foo JN, O'Roak BJ, Zhao H, Larson MG, Simon DB, Newton-Cheh C, State MW, Levy D, Lifton RP
Nature genetics40(5)592-599(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa93346	XM_015475706.2 Latest version!	Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa39773	NM_001206178.1 Latest version!	Bos taurus solute carrier family 12 member 3 (SLC12A3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa28324	XM_005218464.4 Latest version!	Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa28325	XM_005218465.4 Latest version!	Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa28324	XM_005218464.3	Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa28325	XM_005218465.3	Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa93346	XM_015475706.1	Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A3 ( XM_015475706.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331192.1 Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa93346
Clone ID Related Accession (Same CDS sequence)	XM_015475706.2 , XM_015475706.1
Accession Version	XM_015475706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACAAG GATAGCAAGG AAGCCAGGGC CGGCGGGGCC 
CACCTGTCTG TCTCCGGCAC AGTGGACCCC GAGGCCTTGG TGCAGGAGGA GCAGGCCAGC 
ACAGTCTTCC AGTCGGAGCA GGGCAAGAAG ACCATTGACA TCTACTGGCT GTTCGACGAT 
GGAGGCCTCA CCCTCCTCAT CCCCTATCTC CTCGGCCGCA AGAAGAGATG GAGCAAATGC 
AGGATCCGCG TGTTCGTGGG GGGCCAGATC AACAGGATGG ACCAGGAGAG AAAGGCGATC 
ATTTCTCTGC TGAGCAAGTT CCGGTTGGGA TTCCACGAAG TTCACGTCCT TCCCGACATC 
AACCAGAAGC CACGGGCCGA GCACATCAAG CGCTTTGAGG ACATGATTGC ACCCTTCCGC 
CTGAATGATG GCTTCAAGGA CGAGGCCACG GTCACTGAGA TGAGGCGGGA CTGCCCCTGG 
AAGATCTCGG ATGAGGAGAT TAACAAGAAT AGAATCAAGT CCCTGCGGCA GGTGAGGCTG 
AACGAGATTC TGCTGGATTA TTCCCGAGAT GCTGCTCTGG TTGTCATCAC CCTGCCCATA 
GGGAGGAAGG GGAAGTGCCC GAGCTCACTC TACATGGCCT GGCTGGAGAC GCTGTCCCAG 
GACCTCAGAC CTCCAGTCAT CCTGATCCGA GGAAACCAGG AGAACGTGCT CACCTTTTAC 
TGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331192.1
CDS	49..3117
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHKDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_015475706.2
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475706.2

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACAAG GATAGCAAGG AAGCCAGGGC CGGCGGGGCC 
CACCTGTCTG TCTCCGGCAC AGTGGACCCC GAGGCCTTGG TGCAGGAGGA GCAGGCCAGC 
ACAGTCTTCC AGTCGGAGCA GGGCAAGAAG ACCATTGACA TCTACTGGCT GTTCGACGAT 
GGAGGCCTCA CCCTCCTCAT CCCCTATCTC CTCGGCCGCA AGAAGAGATG GAGCAAATGC 
AGGATCCGCG TGTTCGTGGG GGGCCAGATC AACAGGATGG ACCAGGAGAG AAAGGCGATC 
ATTTCTCTGC TGAGCAAGTT CCGGTTGGGA TTCCACGAAG TTCACGTCCT TCCCGACATC 
AACCAGAAGC CACGGGCCGA GCACATCAAG CGCTTTGAGG ACATGATTGC ACCCTTCCGC 
CTGAATGATG GCTTCAAGGA CGAGGCCACG GTCACTGAGA TGAGGCGGGA CTGCCCCTGG 
AAGATCTCGG ATGAGGAGAT TAACAAGAAT AGAATCAAGT CCCTGCGGCA GGTGAGGCTG 
AACGAGATTC TGCTGGATTA TTCCCGAGAT GCTGCTCTGG TTGTCATCAC CCTGCCCATA 
GGGAGGAAGG GGAAGTGCCC GAGCTCACTC TACATGGCCT GGCTGGAGAC GCTGTCCCAG 
GACCTCAGAC CTCCAGTCAT CCTGATCCGA GGAAACCAGG AGAACGTGCT CACCTTTTAC 
TGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( XM_015475706.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331192.1 Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa93346
Clone ID Related Accession (Same CDS sequence)	XM_015475706.2 , XM_015475706.1
Accession Version	XM_015475706.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACAAG GATAGCAAGG AAGCCAGGGC CGGCGGGGCC 
CACCTGTCTG TCTCCGGCAC AGTGGACCCC GAGGCCTTGG TGCAGGAGGA GCAGGCCAGC 
ACAGTCTTCC AGTCGGAGCA GGGCAAGAAG ACCATTGACA TCTACTGGCT GTTCGACGAT 
GGAGGCCTCA CCCTCCTCAT CCCCTATCTC CTCGGCCGCA AGAAGAGATG GAGCAAATGC 
AGGATCCGCG TGTTCGTGGG GGGCCAGATC AACAGGATGG ACCAGGAGAG AAAGGCGATC 
ATTTCTCTGC TGAGCAAGTT CCGGTTGGGA TTCCACGAAG TTCACGTCCT TCCCGACATC 
AACCAGAAGC CACGGGCCGA GCACATCAAG CGCTTTGAGG ACATGATTGC ACCCTTCCGC 
CTGAATGATG GCTTCAAGGA CGAGGCCACG GTCACTGAGA TGAGGCGGGA CTGCCCCTGG 
AAGATCTCGG ATGAGGAGAT TAACAAGAAT AGAATCAAGT CCCTGCGGCA GGTGAGGCTG 
AACGAGATTC TGCTGGATTA TTCCCGAGAT GCTGCTCTGG TTGTCATCAC CCTGCCCATA 
GGGAGGAAGG GGAAGTGCCC GAGCTCACTC TACATGGCCT GGCTGGAGAC GCTGTCCCAG 
GACCTCAGAC CTCCAGTCAT CCTGATCCGA GGAAACCAGG AGAACGTGCT CACCTTTTAC 
TGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331192.1
CDS	48..3116
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHKDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_015475706.1
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475706.1

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACAAG GATAGCAAGG AAGCCAGGGC CGGCGGGGCC 
CACCTGTCTG TCTCCGGCAC AGTGGACCCC GAGGCCTTGG TGCAGGAGGA GCAGGCCAGC 
ACAGTCTTCC AGTCGGAGCA GGGCAAGAAG ACCATTGACA TCTACTGGCT GTTCGACGAT 
GGAGGCCTCA CCCTCCTCAT CCCCTATCTC CTCGGCCGCA AGAAGAGATG GAGCAAATGC 
AGGATCCGCG TGTTCGTGGG GGGCCAGATC AACAGGATGG ACCAGGAGAG AAAGGCGATC 
ATTTCTCTGC TGAGCAAGTT CCGGTTGGGA TTCCACGAAG TTCACGTCCT TCCCGACATC 
AACCAGAAGC CACGGGCCGA GCACATCAAG CGCTTTGAGG ACATGATTGC ACCCTTCCGC 
CTGAATGATG GCTTCAAGGA CGAGGCCACG GTCACTGAGA TGAGGCGGGA CTGCCCCTGG 
AAGATCTCGG ATGAGGAGAT TAACAAGAAT AGAATCAAGT CCCTGCGGCA GGTGAGGCTG 
AACGAGATTC TGCTGGATTA TTCCCGAGAT GCTGCTCTGG TTGTCATCAC CCTGCCCATA 
GGGAGGAAGG GGAAGTGCCC GAGCTCACTC TACATGGCCT GGCTGGAGAC GCTGTCCCAG 
GACCTCAGAC CTCCAGTCAT CCTGATCCGA GGAAACCAGG AGAACGTGCT CACCTTTTAC 
TGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( NM_001206178.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193107.1 Bos taurus solute carrier family 12 member 3 (SLC12A3), mRNA.

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CloneID	OBa39773
Clone ID Related Accession (Same CDS sequence)	NM_001206178.1
Accession Version	NM_001206178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On Apr 20, 2011 this sequence version replaced XM_866019.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145414, SAMN03145420 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACATT AACCCTATGT TTGATCCAGC AGAGGATAGC 
AAGGAAGCCA GGGCCGGCGG GGCCCACCTG TCTGTCTCCG GCACAGTGGA CCCCGAGGCC 
TTGGTGCAGG AGGAGCAGGC CAGCACAGTC TTCCAGTCGG AGCAGGGCAA GAAGACCATT 
GACATCTACT GGCTGTTCGA CGATGGAGGC CTCACCCTCC TCATCCCCTA TCTCCTCGGC 
CGCAAGAAGA GATGGAGCAA ATGCAGGATC CGCGTGTTCG TGGGGGGCCA GATCAACAGG 
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGGTT GGGATTCCAC 
GAAGTTCACG TCCTTCCCGA CATCAACCAG AAGCCACGGG CCGAGCACAT CAAGCGCTTT 
GAGGACATGA TTGCACCCTT CCGCCTGAAT GATGGCTTCA AGGACGAGGC CACGGTCACT 
GAGATGAGGC GGGACTGCCC CTGGAAGATC TCGGATGAGG AGATTAACAA GAATAGAATC 
AAGTCCCTGC GGCAGGTGAG GCTGAACGAG ATTCTGCTGG ATTATTCCCG AGATGCTGCT 
CTGGTTGTCA TCACCCTGCC CATAGGGAGG AAGGGGAAGT GCCCGAGCTC ACTCTACATG 
GCCTGGCTGG AGACGCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC 
CAGGAGAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193107.1
CDS	1..3093
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHINPMFDPAEDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_001206178.1
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 3 (SLC12A3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206178.1

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACATT AACCCTATGT TTGATCCAGC AGAGGATAGC 
AAGGAAGCCA GGGCCGGCGG GGCCCACCTG TCTGTCTCCG GCACAGTGGA CCCCGAGGCC 
TTGGTGCAGG AGGAGCAGGC CAGCACAGTC TTCCAGTCGG AGCAGGGCAA GAAGACCATT 
GACATCTACT GGCTGTTCGA CGATGGAGGC CTCACCCTCC TCATCCCCTA TCTCCTCGGC 
CGCAAGAAGA GATGGAGCAA ATGCAGGATC CGCGTGTTCG TGGGGGGCCA GATCAACAGG 
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGGTT GGGATTCCAC 
GAAGTTCACG TCCTTCCCGA CATCAACCAG AAGCCACGGG CCGAGCACAT CAAGCGCTTT 
GAGGACATGA TTGCACCCTT CCGCCTGAAT GATGGCTTCA AGGACGAGGC CACGGTCACT 
GAGATGAGGC GGGACTGCCC CTGGAAGATC TCGGATGAGG AGATTAACAA GAATAGAATC 
AAGTCCCTGC GGCAGGTGAG GCTGAACGAG ATTCTGCTGG ATTATTCCCG AGATGCTGCT 
CTGGTTGTCA TCACCCTGCC CATAGGGAGG AAGGGGAAGT GCCCGAGCTC ACTCTACATG 
GCCTGGCTGG AGACGCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC 
CAGGAGAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( XM_005218464.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218521.1 Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X1, mRNA.

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CloneID	OBa28324
Clone ID Related Accession (Same CDS sequence)	XM_005218464.3 , XM_005218464.4
Accession Version	XM_005218464.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGGAAGGCAG CCACCTGCAT 
GCCCTTGCCT TTGACAGCCG GCCCAGCCAT GAGATGACTG ATGGGCTGGT GGAGGATGAG 
GCAGGTATCA ATGGTGAGAA GAACCCTGAG GAGCCCGTGC GCTTTGGCTG GGTCAAAGGG 
GTGATGATCC GCTGCATGCT CAATATCTGG GGTGTGATTC TGTATCTGCG GCTCCCCTGG 
ATTACCGCCC AGGCGGGCAT TGTCCTGACC TGGATCATCA TCCTGCTCTC GGTCACCGTG 
ACCTCCATCA CGGGCCTCTC CATCTCCGCC ATCTCCACCA ACGGCAAGGT CAAGTCAGGT 
GGCACGTACT TCCTCATCTC CCGGAGTCTT GGCCCAGAGC TAGGCGGCTC CATTGGCCTC 
ATTTTTGCTT TCGCCAATGC CGTGGGCGTG GCCATGCACA CCGTGGGCTT TGCAGAGACT 
GTGCGGGACC TGCTCCAGGA GTATGGCTCG CCCATCGTGG ACCCCACCAA TGACATCCGC 
ATCATCGGCG TGGTCACCGT CACTGTTCTG CTGGCCATCT CCCTTGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT CTTCTTCCTC GTCATCATGG TCTCGTTCGC CAACTATCTG 
GTGGGGACAC TGATCCCCCC ATCCGAGGAG AAGGCCTCCA AAGGCTTCTT CAGCTACCGA 
GCGGACATTT TTGTCCAAAA CTTGGTGCCT GAATGGAGAG GCATGGACGG CAGCTTCTTC 
GGGATGTTTT CCATCTTCTT CCCCTCGGCC ACAGGTATCC TGGCAGGAGC CAACATCTCT 
GGGGACCTCA AGGACCCTGC AGTAGCCATC CCCAAGGGAA CACTCATGGC CATTTTCTGG 
ACCACTGTCT CCTACCTGGC CATCTCGGCC ACCATCGGCT CCTGTGTGGT TCGAGATGCC 
TCTGGGGGGC TGAATGACAC GGTGACCCCC GGCTCAGGCG CCTGCGAGGG GCTGGCCTGT 
GGCTACGGCT GGAACTTCAC CGAGTGCGCC CAGCAGCGCA GCTGCCGCTA CGGCCTCATC 
AACTACTATC AGACTATGAG CATGGTGTCA GGCTTCGCAC CCCTGATCAC AGCTGGCATC 
TTTGGGGCCA CCCTTTCCTC TGCCCTGGCC TGTCTCGTCT CTGCCGCCAA AGTCTTCCAG 
TGCCTGTGCC TGGACCAGCT GTACCCGCTC ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAACGAGC CGGTGCGTGG CTACCTGCTG GCCTATGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT CGCCCCCATC ATCTCCAACT TCTTCCTGTG CTCCTACGCC 
CTCATCAACT TCAGCTGCTT CCATGCCTCC ATCACCAACT CTCCTGGGTG GAGACCCTCG 
TTCCGATACT ACAGCAAGTG GGCAGCGCTG TTTGGGGCGA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATCGGCG TGGTCCTCTT CCTCCTGCTC 
TATGTGATCT ACAAGAAGCC AGAGGTGAAC TGGGGCTCCT CGGTACAGGC GGGCTCCTAC 
AACCTGGCCC TGAGCTACTC TGTGGGTCTC AATGAGGTGG AGGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTC 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCATGTGCT CATGGGCCCC 
CGCAAACAGA GGATGCCTGA GCTCCGGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAAGCCTT CTACTCCGAC GTGCTCGCCG AGGACCTCCG CAGCGGCGTC 
CAGGTTCTCA TGCAGGCCGC AGGTCTCGGG AGGATGAAGC CCAACATTCT GGTGATCGGA 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCA GCCACGGTGG AAGACTACAT TGGCATCCTG 
CACGACGCTT TTGATCTCAA CTACGGTGTC TGTGTCATGA GGATGCGTGA GGGCCTCAAC 
ATCTCAGAGG TGATGCAGGC GCACATTAAC CCTATGTTTG ATCCAGCAGA GGATAGCAAG 
GAAGCCAGGG CCGGCGGGGC CCACCTGTCT GTCTCCGGCA CAGTGGACCC CGAGGCCTTG 
GTGCAGGAGG AGCAGGCCAG CACAGTCTTC CAGTCGGAGC AGGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTCGACGA TGGAGGCCTC ACCCTCCTCA TCCCCTATCT CCTCGGCCGC 
AAGAAGAGAT GGAGCAAATG CAGGATCCGC GTGTTCGTGG GGGGCCAGAT CAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGGTTGGG ATTCCACGAA 
GTTCACGTCC TTCCCGACAT CAACCAGAAG CCACGGGCCG AGCACATCAA GCGCTTTGAG 
GACATGATTG CACCCTTCCG CCTGAATGAT GGCTTCAAGG ACGAGGCCAC GGTCACTGAG 
ATGAGGCGGG ACTGCCCCTG GAAGATCTCG GATGAGGAGA TTAACAAGAA TAGAATCAAG 
TCCCTGCGGC AGGTGAGGCT GAACGAGATT CTGCTGGATT ATTCCCGAGA TGCTGCTCTG 
GTTGTCATCA CCCTGCCCAT AGGGAGGAAG GGGAAGTGCC CGAGCTCACT CTACATGGCC 
TGGCTGGAGA CGCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218521.1
CDS	48..3137
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHINPMFDPAEDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_005218464.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218464.3

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGGAAGGCAG CCACCTGCAT 
GCCCTTGCCT TTGACAGCCG GCCCAGCCAT GAGATGACTG ATGGGCTGGT GGAGGATGAG 
GCAGGTATCA ATGGTGAGAA GAACCCTGAG GAGCCCGTGC GCTTTGGCTG GGTCAAAGGG 
GTGATGATCC GCTGCATGCT CAATATCTGG GGTGTGATTC TGTATCTGCG GCTCCCCTGG 
ATTACCGCCC AGGCGGGCAT TGTCCTGACC TGGATCATCA TCCTGCTCTC GGTCACCGTG 
ACCTCCATCA CGGGCCTCTC CATCTCCGCC ATCTCCACCA ACGGCAAGGT CAAGTCAGGT 
GGCACGTACT TCCTCATCTC CCGGAGTCTT GGCCCAGAGC TAGGCGGCTC CATTGGCCTC 
ATTTTTGCTT TCGCCAATGC CGTGGGCGTG GCCATGCACA CCGTGGGCTT TGCAGAGACT 
GTGCGGGACC TGCTCCAGGA GTATGGCTCG CCCATCGTGG ACCCCACCAA TGACATCCGC 
ATCATCGGCG TGGTCACCGT CACTGTTCTG CTGGCCATCT CCCTTGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT CTTCTTCCTC GTCATCATGG TCTCGTTCGC CAACTATCTG 
GTGGGGACAC TGATCCCCCC ATCCGAGGAG AAGGCCTCCA AAGGCTTCTT CAGCTACCGA 
GCGGACATTT TTGTCCAAAA CTTGGTGCCT GAATGGAGAG GCATGGACGG CAGCTTCTTC 
GGGATGTTTT CCATCTTCTT CCCCTCGGCC ACAGGTATCC TGGCAGGAGC CAACATCTCT 
GGGGACCTCA AGGACCCTGC AGTAGCCATC CCCAAGGGAA CACTCATGGC CATTTTCTGG 
ACCACTGTCT CCTACCTGGC CATCTCGGCC ACCATCGGCT CCTGTGTGGT TCGAGATGCC 
TCTGGGGGGC TGAATGACAC GGTGACCCCC GGCTCAGGCG CCTGCGAGGG GCTGGCCTGT 
GGCTACGGCT GGAACTTCAC CGAGTGCGCC CAGCAGCGCA GCTGCCGCTA CGGCCTCATC 
AACTACTATC AGACTATGAG CATGGTGTCA GGCTTCGCAC CCCTGATCAC AGCTGGCATC 
TTTGGGGCCA CCCTTTCCTC TGCCCTGGCC TGTCTCGTCT CTGCCGCCAA AGTCTTCCAG 
TGCCTGTGCC TGGACCAGCT GTACCCGCTC ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAACGAGC CGGTGCGTGG CTACCTGCTG GCCTATGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT CGCCCCCATC ATCTCCAACT TCTTCCTGTG CTCCTACGCC 
CTCATCAACT TCAGCTGCTT CCATGCCTCC ATCACCAACT CTCCTGGGTG GAGACCCTCG 
TTCCGATACT ACAGCAAGTG GGCAGCGCTG TTTGGGGCGA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATCGGCG TGGTCCTCTT CCTCCTGCTC 
TATGTGATCT ACAAGAAGCC AGAGGTGAAC TGGGGCTCCT CGGTACAGGC GGGCTCCTAC 
AACCTGGCCC TGAGCTACTC TGTGGGTCTC AATGAGGTGG AGGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTC 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCATGTGCT CATGGGCCCC 
CGCAAACAGA GGATGCCTGA GCTCCGGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAAGCCTT CTACTCCGAC GTGCTCGCCG AGGACCTCCG CAGCGGCGTC 
CAGGTTCTCA TGCAGGCCGC AGGTCTCGGG AGGATGAAGC CCAACATTCT GGTGATCGGA 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCA GCCACGGTGG AAGACTACAT TGGCATCCTG 
CACGACGCTT TTGATCTCAA CTACGGTGTC TGTGTCATGA GGATGCGTGA GGGCCTCAAC 
ATCTCAGAGG TGATGCAGGC GCACATTAAC CCTATGTTTG ATCCAGCAGA GGATAGCAAG 
GAAGCCAGGG CCGGCGGGGC CCACCTGTCT GTCTCCGGCA CAGTGGACCC CGAGGCCTTG 
GTGCAGGAGG AGCAGGCCAG CACAGTCTTC CAGTCGGAGC AGGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTCGACGA TGGAGGCCTC ACCCTCCTCA TCCCCTATCT CCTCGGCCGC 
AAGAAGAGAT GGAGCAAATG CAGGATCCGC GTGTTCGTGG GGGGCCAGAT CAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGGTTGGG ATTCCACGAA 
GTTCACGTCC TTCCCGACAT CAACCAGAAG CCACGGGCCG AGCACATCAA GCGCTTTGAG 
GACATGATTG CACCCTTCCG CCTGAATGAT GGCTTCAAGG ACGAGGCCAC GGTCACTGAG 
ATGAGGCGGG ACTGCCCCTG GAAGATCTCG GATGAGGAGA TTAACAAGAA TAGAATCAAG 
TCCCTGCGGC AGGTGAGGCT GAACGAGATT CTGCTGGATT ATTCCCGAGA TGCTGCTCTG 
GTTGTCATCA CCCTGCCCAT AGGGAGGAAG GGGAAGTGCC CGAGCTCACT CTACATGGCC 
TGGCTGGAGA CGCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( XM_005218464.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218521.1 Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28324
Clone ID Related Accession (Same CDS sequence)	XM_005218464.3 , XM_005218464.4
Accession Version	XM_005218464.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218464.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGGAAGGCAG CCACCTGCAT 
GCCCTTGCCT TTGACAGCCG GCCCAGCCAT GAGATGACTG ATGGGCTGGT GGAGGATGAG 
GCAGGTATCA ATGGTGAGAA GAACCCTGAG GAGCCCGTGC GCTTTGGCTG GGTCAAAGGG 
GTGATGATCC GCTGCATGCT CAATATCTGG GGTGTGATTC TGTATCTGCG GCTCCCCTGG 
ATTACCGCCC AGGCGGGCAT TGTCCTGACC TGGATCATCA TCCTGCTCTC GGTCACCGTG 
ACCTCCATCA CGGGCCTCTC CATCTCCGCC ATCTCCACCA ACGGCAAGGT CAAGTCAGGT 
GGCACGTACT TCCTCATCTC CCGGAGTCTT GGCCCAGAGC TAGGCGGCTC CATTGGCCTC 
ATTTTTGCTT TCGCCAATGC CGTGGGCGTG GCCATGCACA CCGTGGGCTT TGCAGAGACT 
GTGCGGGACC TGCTCCAGGA GTATGGCTCG CCCATCGTGG ACCCCACCAA TGACATCCGC 
ATCATCGGCG TGGTCACCGT CACTGTTCTG CTGGCCATCT CCCTTGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT CTTCTTCCTC GTCATCATGG TCTCGTTCGC CAACTATCTG 
GTGGGGACAC TGATCCCCCC ATCCGAGGAG AAGGCCTCCA AAGGCTTCTT CAGCTACCGA 
GCGGACATTT TTGTCCAAAA CTTGGTGCCT GAATGGAGAG GCATGGACGG CAGCTTCTTC 
GGGATGTTTT CCATCTTCTT CCCCTCGGCC ACAGGTATCC TGGCAGGAGC CAACATCTCT 
GGGGACCTCA AGGACCCTGC AGTAGCCATC CCCAAGGGAA CACTCATGGC CATTTTCTGG 
ACCACTGTCT CCTACCTGGC CATCTCGGCC ACCATCGGCT CCTGTGTGGT TCGAGATGCC 
TCTGGGGGGC TGAATGACAC GGTGACCCCC GGCTCAGGCG CCTGCGAGGG GCTGGCCTGT 
GGCTACGGCT GGAACTTCAC CGAGTGCGCC CAGCAGCGCA GCTGCCGCTA CGGCCTCATC 
AACTACTATC AGACTATGAG CATGGTGTCA GGCTTCGCAC CCCTGATCAC AGCTGGCATC 
TTTGGGGCCA CCCTTTCCTC TGCCCTGGCC TGTCTCGTCT CTGCCGCCAA AGTCTTCCAG 
TGCCTGTGCC TGGACCAGCT GTACCCGCTC ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAACGAGC CGGTGCGTGG CTACCTGCTG GCCTATGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT CGCCCCCATC ATCTCCAACT TCTTCCTGTG CTCCTACGCC 
CTCATCAACT TCAGCTGCTT CCATGCCTCC ATCACCAACT CTCCTGGGTG GAGACCCTCG 
TTCCGATACT ACAGCAAGTG GGCAGCGCTG TTTGGGGCGA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATCGGCG TGGTCCTCTT CCTCCTGCTC 
TATGTGATCT ACAAGAAGCC AGAGGTGAAC TGGGGCTCCT CGGTACAGGC GGGCTCCTAC 
AACCTGGCCC TGAGCTACTC TGTGGGTCTC AATGAGGTGG AGGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTC 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCATGTGCT CATGGGCCCC 
CGCAAACAGA GGATGCCTGA GCTCCGGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAAGCCTT CTACTCCGAC GTGCTCGCCG AGGACCTCCG CAGCGGCGTC 
CAGGTTCTCA TGCAGGCCGC AGGTCTCGGG AGGATGAAGC CCAACATTCT GGTGATCGGA 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCA GCCACGGTGG AAGACTACAT TGGCATCCTG 
CACGACGCTT TTGATCTCAA CTACGGTGTC TGTGTCATGA GGATGCGTGA GGGCCTCAAC 
ATCTCAGAGG TGATGCAGGC GCACATTAAC CCTATGTTTG ATCCAGCAGA GGATAGCAAG 
GAAGCCAGGG CCGGCGGGGC CCACCTGTCT GTCTCCGGCA CAGTGGACCC CGAGGCCTTG 
GTGCAGGAGG AGCAGGCCAG CACAGTCTTC CAGTCGGAGC AGGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTCGACGA TGGAGGCCTC ACCCTCCTCA TCCCCTATCT CCTCGGCCGC 
AAGAAGAGAT GGAGCAAATG CAGGATCCGC GTGTTCGTGG GGGGCCAGAT CAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGGTTGGG ATTCCACGAA 
GTTCACGTCC TTCCCGACAT CAACCAGAAG CCACGGGCCG AGCACATCAA GCGCTTTGAG 
GACATGATTG CACCCTTCCG CCTGAATGAT GGCTTCAAGG ACGAGGCCAC GGTCACTGAG 
ATGAGGCGGG ACTGCCCCTG GAAGATCTCG GATGAGGAGA TTAACAAGAA TAGAATCAAG 
TCCCTGCGGC AGGTGAGGCT GAACGAGATT CTGCTGGATT ATTCCCGAGA TGCTGCTCTG 
GTTGTCATCA CCCTGCCCAT AGGGAGGAAG GGGAAGTGCC CGAGCTCACT CTACATGGCC 
TGGCTGGAGA CGCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218521.1
CDS	49..3138
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHINPMFDPAEDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_005218464.4
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218464.4

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGGAAGGCAG CCACCTGCAT 
GCCCTTGCCT TTGACAGCCG GCCCAGCCAT GAGATGACTG ATGGGCTGGT GGAGGATGAG 
GCAGGTATCA ATGGTGAGAA GAACCCTGAG GAGCCCGTGC GCTTTGGCTG GGTCAAAGGG 
GTGATGATCC GCTGCATGCT CAATATCTGG GGTGTGATTC TGTATCTGCG GCTCCCCTGG 
ATTACCGCCC AGGCGGGCAT TGTCCTGACC TGGATCATCA TCCTGCTCTC GGTCACCGTG 
ACCTCCATCA CGGGCCTCTC CATCTCCGCC ATCTCCACCA ACGGCAAGGT CAAGTCAGGT 
GGCACGTACT TCCTCATCTC CCGGAGTCTT GGCCCAGAGC TAGGCGGCTC CATTGGCCTC 
ATTTTTGCTT TCGCCAATGC CGTGGGCGTG GCCATGCACA CCGTGGGCTT TGCAGAGACT 
GTGCGGGACC TGCTCCAGGA GTATGGCTCG CCCATCGTGG ACCCCACCAA TGACATCCGC 
ATCATCGGCG TGGTCACCGT CACTGTTCTG CTGGCCATCT CCCTTGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT CTTCTTCCTC GTCATCATGG TCTCGTTCGC CAACTATCTG 
GTGGGGACAC TGATCCCCCC ATCCGAGGAG AAGGCCTCCA AAGGCTTCTT CAGCTACCGA 
GCGGACATTT TTGTCCAAAA CTTGGTGCCT GAATGGAGAG GCATGGACGG CAGCTTCTTC 
GGGATGTTTT CCATCTTCTT CCCCTCGGCC ACAGGTATCC TGGCAGGAGC CAACATCTCT 
GGGGACCTCA AGGACCCTGC AGTAGCCATC CCCAAGGGAA CACTCATGGC CATTTTCTGG 
ACCACTGTCT CCTACCTGGC CATCTCGGCC ACCATCGGCT CCTGTGTGGT TCGAGATGCC 
TCTGGGGGGC TGAATGACAC GGTGACCCCC GGCTCAGGCG CCTGCGAGGG GCTGGCCTGT 
GGCTACGGCT GGAACTTCAC CGAGTGCGCC CAGCAGCGCA GCTGCCGCTA CGGCCTCATC 
AACTACTATC AGACTATGAG CATGGTGTCA GGCTTCGCAC CCCTGATCAC AGCTGGCATC 
TTTGGGGCCA CCCTTTCCTC TGCCCTGGCC TGTCTCGTCT CTGCCGCCAA AGTCTTCCAG 
TGCCTGTGCC TGGACCAGCT GTACCCGCTC ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAACGAGC CGGTGCGTGG CTACCTGCTG GCCTATGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT CGCCCCCATC ATCTCCAACT TCTTCCTGTG CTCCTACGCC 
CTCATCAACT TCAGCTGCTT CCATGCCTCC ATCACCAACT CTCCTGGGTG GAGACCCTCG 
TTCCGATACT ACAGCAAGTG GGCAGCGCTG TTTGGGGCGA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATCGGCG TGGTCCTCTT CCTCCTGCTC 
TATGTGATCT ACAAGAAGCC AGAGGTGAAC TGGGGCTCCT CGGTACAGGC GGGCTCCTAC 
AACCTGGCCC TGAGCTACTC TGTGGGTCTC AATGAGGTGG AGGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTC 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCATGTGCT CATGGGCCCC 
CGCAAACAGA GGATGCCTGA GCTCCGGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAAGCCTT CTACTCCGAC GTGCTCGCCG AGGACCTCCG CAGCGGCGTC 
CAGGTTCTCA TGCAGGCCGC AGGTCTCGGG AGGATGAAGC CCAACATTCT GGTGATCGGA 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCA GCCACGGTGG AAGACTACAT TGGCATCCTG 
CACGACGCTT TTGATCTCAA CTACGGTGTC TGTGTCATGA GGATGCGTGA GGGCCTCAAC 
ATCTCAGAGG TGATGCAGGC GCACATTAAC CCTATGTTTG ATCCAGCAGA GGATAGCAAG 
GAAGCCAGGG CCGGCGGGGC CCACCTGTCT GTCTCCGGCA CAGTGGACCC CGAGGCCTTG 
GTGCAGGAGG AGCAGGCCAG CACAGTCTTC CAGTCGGAGC AGGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTCGACGA TGGAGGCCTC ACCCTCCTCA TCCCCTATCT CCTCGGCCGC 
AAGAAGAGAT GGAGCAAATG CAGGATCCGC GTGTTCGTGG GGGGCCAGAT CAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGGTTGGG ATTCCACGAA 
GTTCACGTCC TTCCCGACAT CAACCAGAAG CCACGGGCCG AGCACATCAA GCGCTTTGAG 
GACATGATTG CACCCTTCCG CCTGAATGAT GGCTTCAAGG ACGAGGCCAC GGTCACTGAG 
ATGAGGCGGG ACTGCCCCTG GAAGATCTCG GATGAGGAGA TTAACAAGAA TAGAATCAAG 
TCCCTGCGGC AGGTGAGGCT GAACGAGATT CTGCTGGATT ATTCCCGAGA TGCTGCTCTG 
GTTGTCATCA CCCTGCCCAT AGGGAGGAAG GGGAAGTGCC CGAGCTCACT CTACATGGCC 
TGGCTGGAGA CGCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( XM_005218465.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218522.1 Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28325
Clone ID Related Accession (Same CDS sequence)	XM_005218465.4 , XM_005218465.3
Accession Version	XM_005218465.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218465.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACACA GAGGATAGCA AGGAAGCCAG GGCCGGCGGG 
GCCCACCTGT CTGTCTCCGG CACAGTGGAC CCCGAGGCCT TGGTGCAGGA GGAGCAGGCC 
AGCACAGTCT TCCAGTCGGA GCAGGGCAAG AAGACCATTG ACATCTACTG GCTGTTCGAC 
GATGGAGGCC TCACCCTCCT CATCCCCTAT CTCCTCGGCC GCAAGAAGAG ATGGAGCAAA 
TGCAGGATCC GCGTGTTCGT GGGGGGCCAG ATCAACAGGA TGGACCAGGA GAGAAAGGCG 
ATCATTTCTC TGCTGAGCAA GTTCCGGTTG GGATTCCACG AAGTTCACGT CCTTCCCGAC 
ATCAACCAGA AGCCACGGGC CGAGCACATC AAGCGCTTTG AGGACATGAT TGCACCCTTC 
CGCCTGAATG ATGGCTTCAA GGACGAGGCC ACGGTCACTG AGATGAGGCG GGACTGCCCC 
TGGAAGATCT CGGATGAGGA GATTAACAAG AATAGAATCA AGTCCCTGCG GCAGGTGAGG 
CTGAACGAGA TTCTGCTGGA TTATTCCCGA GATGCTGCTC TGGTTGTCAT CACCCTGCCC 
ATAGGGAGGA AGGGGAAGTG CCCGAGCTCA CTCTACATGG CCTGGCTGGA GACGCTGTCC 
CAGGACCTCA GACCTCCAGT CATCCTGATC CGAGGAAACC AGGAGAACGT GCTCACCTTT 
TACTGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218522.1
CDS	49..3120
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHTEDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_005218465.4
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 3 (SLC12A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218465.4

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACACA GAGGATAGCA AGGAAGCCAG GGCCGGCGGG 
GCCCACCTGT CTGTCTCCGG CACAGTGGAC CCCGAGGCCT TGGTGCAGGA GGAGCAGGCC 
AGCACAGTCT TCCAGTCGGA GCAGGGCAAG AAGACCATTG ACATCTACTG GCTGTTCGAC 
GATGGAGGCC TCACCCTCCT CATCCCCTAT CTCCTCGGCC GCAAGAAGAG ATGGAGCAAA 
TGCAGGATCC GCGTGTTCGT GGGGGGCCAG ATCAACAGGA TGGACCAGGA GAGAAAGGCG 
ATCATTTCTC TGCTGAGCAA GTTCCGGTTG GGATTCCACG AAGTTCACGT CCTTCCCGAC 
ATCAACCAGA AGCCACGGGC CGAGCACATC AAGCGCTTTG AGGACATGAT TGCACCCTTC 
CGCCTGAATG ATGGCTTCAA GGACGAGGCC ACGGTCACTG AGATGAGGCG GGACTGCCCC 
TGGAAGATCT CGGATGAGGA GATTAACAAG AATAGAATCA AGTCCCTGCG GCAGGTGAGG 
CTGAACGAGA TTCTGCTGGA TTATTCCCGA GATGCTGCTC TGGTTGTCAT CACCCTGCCC 
ATAGGGAGGA AGGGGAAGTG CCCGAGCTCA CTCTACATGG CCTGGCTGGA GACGCTGTCC 
CAGGACCTCA GACCTCCAGT CATCCTGATC CGAGGAAACC AGGAGAACGT GCTCACCTTT 
TACTGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( XM_005218465.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218522.1 Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X2, mRNA.

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CloneID	OBa28325
Clone ID Related Accession (Same CDS sequence)	XM_005218465.4 , XM_005218465.3
Accession Version	XM_005218465.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACACA GAGGATAGCA AGGAAGCCAG GGCCGGCGGG 
GCCCACCTGT CTGTCTCCGG CACAGTGGAC CCCGAGGCCT TGGTGCAGGA GGAGCAGGCC 
AGCACAGTCT TCCAGTCGGA GCAGGGCAAG AAGACCATTG ACATCTACTG GCTGTTCGAC 
GATGGAGGCC TCACCCTCCT CATCCCCTAT CTCCTCGGCC GCAAGAAGAG ATGGAGCAAA 
TGCAGGATCC GCGTGTTCGT GGGGGGCCAG ATCAACAGGA TGGACCAGGA GAGAAAGGCG 
ATCATTTCTC TGCTGAGCAA GTTCCGGTTG GGATTCCACG AAGTTCACGT CCTTCCCGAC 
ATCAACCAGA AGCCACGGGC CGAGCACATC AAGCGCTTTG AGGACATGAT TGCACCCTTC 
CGCCTGAATG ATGGCTTCAA GGACGAGGCC ACGGTCACTG AGATGAGGCG GGACTGCCCC 
TGGAAGATCT CGGATGAGGA GATTAACAAG AATAGAATCA AGTCCCTGCG GCAGGTGAGG 
CTGAACGAGA TTCTGCTGGA TTATTCCCGA GATGCTGCTC TGGTTGTCAT CACCCTGCCC 
ATAGGGAGGA AGGGGAAGTG CCCGAGCTCA CTCTACATGG CCTGGCTGGA GACGCTGTCC 
CAGGACCTCA GACCTCCAGT CATCCTGATC CGAGGAAACC AGGAGAACGT GCTCACCTTT 
TACTGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218522.1
CDS	48..3119
Translation	MAELPVSEAPRDPALCSGRFTISTLLGGEEPPPPAAYDSSHPSHLTHGNTFYNRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDSRPSHEMTDGLVEDEAGINGEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGSPIVDPTNDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEEKASKGFFSYRADIFVQNLVPEWRGMDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGGLNDTVTPGSGACEGLACGYGWNFTECAQQRSCRYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCLDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLMGPRKQRMPELRLIANGHTKWLNKRKIKAFYSDVLAEDLRSGVQVLMQAAGLGRMKPNILVIGFKKNWQSAHPATVEDYIGILHDAFDLNYGVCVMRMREGLNISEVMQAHTEDSKEARAGGAHLSVSGTVDPEALVQEEQASTVFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCRIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHVLPDINQKPRAEHIKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_005218465.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/chloride transporter), member 3 (SLC12A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218465.3

ORF Insert Sequence:

ATGGCAGAGC TGCCAGTGTC GGAGGCGCCC AGGGACCCTG CTCTGTGCAG TGGGCGCTTC 
ACCATCAGCA CATTGCTGGG GGGCGAAGAA CCGCCCCCAC CGGCCGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CGGCAATACC TTCTACAATC GCACCTTTGG CTACAACACT 
ATCGATGTGG TGCCCGCCTA CGAGCACTAT GCCAACAGCG CCCTGCCTGG TGAGCCCCGA 
AAGGTCCGGC CCACGCTGGC CGACCTGCAC TCCTTCCTTA AGCAGGAAGG CAGCCACCTG 
CATGCCCTTG CCTTTGACAG CCGGCCCAGC CATGAGATGA CTGATGGGCT GGTGGAGGAT 
GAGGCAGGTA TCAATGGTGA GAAGAACCCT GAGGAGCCCG TGCGCTTTGG CTGGGTCAAA 
GGGGTGATGA TCCGCTGCAT GCTCAATATC TGGGGTGTGA TTCTGTATCT GCGGCTCCCC 
TGGATTACCG CCCAGGCGGG CATTGTCCTG ACCTGGATCA TCATCCTGCT CTCGGTCACC 
GTGACCTCCA TCACGGGCCT CTCCATCTCC GCCATCTCCA CCAACGGCAA GGTCAAGTCA 
GGTGGCACGT ACTTCCTCAT CTCCCGGAGT CTTGGCCCAG AGCTAGGCGG CTCCATTGGC 
CTCATTTTTG CTTTCGCCAA TGCCGTGGGC GTGGCCATGC ACACCGTGGG CTTTGCAGAG 
ACTGTGCGGG ACCTGCTCCA GGAGTATGGC TCGCCCATCG TGGACCCCAC CAATGACATC 
CGCATCATCG GCGTGGTCAC CGTCACTGTT CTGCTGGCCA TCTCCCTTGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTCTTCTTC CTCGTCATCA TGGTCTCGTT CGCCAACTAT 
CTGGTGGGGA CACTGATCCC CCCATCCGAG GAGAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGAGCGGACA TTTTTGTCCA AAACTTGGTG CCTGAATGGA GAGGCATGGA CGGCAGCTTC 
TTCGGGATGT TTTCCATCTT CTTCCCCTCG GCCACAGGTA TCCTGGCAGG AGCCAACATC 
TCTGGGGACC TCAAGGACCC TGCAGTAGCC ATCCCCAAGG GAACACTCAT GGCCATTTTC 
TGGACCACTG TCTCCTACCT GGCCATCTCG GCCACCATCG GCTCCTGTGT GGTTCGAGAT 
GCCTCTGGGG GGCTGAATGA CACGGTGACC CCCGGCTCAG GCGCCTGCGA GGGGCTGGCC 
TGTGGCTACG GCTGGAACTT CACCGAGTGC GCCCAGCAGC GCAGCTGCCG CTACGGCCTC 
ATCAACTACT ATCAGACTAT GAGCATGGTG TCAGGCTTCG CACCCCTGAT CACAGCTGGC 
ATCTTTGGGG CCACCCTTTC CTCTGCCCTG GCCTGTCTCG TCTCTGCCGC CAAAGTCTTC 
CAGTGCCTGT GCCTGGACCA GCTGTACCCG CTCATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAACG AGCCGGTGCG TGGCTACCTG CTGGCCTATG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATCGCCCCC ATCATCTCCA ACTTCTTCCT GTGCTCCTAC 
GCCCTCATCA ACTTCAGCTG CTTCCATGCC TCCATCACCA ACTCTCCTGG GTGGAGACCC 
TCGTTCCGAT ACTACAGCAA GTGGGCAGCG CTGTTTGGGG CGATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATCG GCGTGGTCCT CTTCCTCCTG 
CTCTATGTGA TCTACAAGAA GCCAGAGGTG AACTGGGGCT CCTCGGTACA GGCGGGCTCC 
TACAACCTGG CCCTGAGCTA CTCTGTGGGT CTCAATGAGG TGGAGGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTCGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCATGT GCTCATGGGC 
CCCCGCAAAC AGAGGATGCC TGAGCTCCGG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAAGC CTTCTACTCC GACGTGCTCG CCGAGGACCT CCGCAGCGGC 
GTCCAGGTTC TCATGCAGGC CGCAGGTCTC GGGAGGATGA AGCCCAACAT TCTGGTGATC 
GGATTCAAGA AGAACTGGCA GTCGGCTCAC CCAGCCACGG TGGAAGACTA CATTGGCATC 
CTGCACGACG CTTTTGATCT CAACTACGGT GTCTGTGTCA TGAGGATGCG TGAGGGCCTC 
AACATCTCAG AGGTGATGCA GGCGCACACA GAGGATAGCA AGGAAGCCAG GGCCGGCGGG 
GCCCACCTGT CTGTCTCCGG CACAGTGGAC CCCGAGGCCT TGGTGCAGGA GGAGCAGGCC 
AGCACAGTCT TCCAGTCGGA GCAGGGCAAG AAGACCATTG ACATCTACTG GCTGTTCGAC 
GATGGAGGCC TCACCCTCCT CATCCCCTAT CTCCTCGGCC GCAAGAAGAG ATGGAGCAAA 
TGCAGGATCC GCGTGTTCGT GGGGGGCCAG ATCAACAGGA TGGACCAGGA GAGAAAGGCG 
ATCATTTCTC TGCTGAGCAA GTTCCGGTTG GGATTCCACG AAGTTCACGT CCTTCCCGAC 
ATCAACCAGA AGCCACGGGC CGAGCACATC AAGCGCTTTG AGGACATGAT TGCACCCTTC 
CGCCTGAATG ATGGCTTCAA GGACGAGGCC ACGGTCACTG AGATGAGGCG GGACTGCCCC 
TGGAAGATCT CGGATGAGGA GATTAACAAG AATAGAATCA AGTCCCTGCG GCAGGTGAGG 
CTGAACGAGA TTCTGCTGGA TTATTCCCGA GATGCTGCTC TGGTTGTCAT CACCCTGCCC 
ATAGGGAGGA AGGGGAAGTG CCCGAGCTCA CTCTACATGG CCTGGCTGGA GACGCTGTCC 
CAGGACCTCA GACCTCCAGT CATCCTGATC CGAGGAAACC AGGAGAACGT GCTCACCTTT 
TACTGCCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Rare independent mutations in renal salt handling genes contribute to blood pressure variation.
Nature genetics40(5)592-599(2008 May)
Ji W,Foo JN,O'Roak BJ,Zhao H,Larson MG,Simon DB,Newton-Cheh C,State MW,Levy D,Lifton RP

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SLC12A3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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