PC gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005226988.3	XP_005227045.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226992.3	XP_005227049.1	pyruvate carboxylase, mitochondrial isoform X2
XM_024986991.1	XP_024842759.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226990.4	XP_005227047.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226991.3	XP_005227048.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226993.3	XP_005227050.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226994.3	XP_005227051.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226994.4	XP_005227051.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226990.3	XP_005227047.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226987.4	XP_005227044.1	pyruvate carboxylase, mitochondrial isoform X1
XM_005226988.4	XP_005227045.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226991.4	XP_005227048.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226987.3	XP_005227044.1	pyruvate carboxylase, mitochondrial isoform X1
XM_005226989.3	XP_005227046.1	pyruvate carboxylase, mitochondrial isoform X2
XM_015461252.1	XP_015316738.1	pyruvate carboxylase, mitochondrial isoform X2
XM_015461253.1	XP_015316739.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226989.4	XP_005227046.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005226993.4	XP_005227050.1	pyruvate carboxylase, mitochondrial isoform X2
NM_177946.4	NP_808815.2	pyruvate carboxylase, mitochondrial precursor
XM_005226992.4	XP_005227049.1	pyruvate carboxylase, mitochondrial isoform X2
XM_015461252.2	XP_015316738.1	pyruvate carboxylase, mitochondrial isoform X2
XM_024986993.1	XP_024842761.1	pyruvate carboxylase, mitochondrial isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa231848	XM_005226987.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226994.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226990.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226991.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226989.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_015461252.2 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226992.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226988.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_024986993.1 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_024986991.1 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa231849	XM_005226993.4 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa01319	NM_177946.4 Latest version!	Bos taurus pyruvate carboxylase (PC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34722	XM_005226987.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226993.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226991.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226994.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226988.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226990.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_015461253.1 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_015461252.1 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226989.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa34723	XM_005226992.3 Latest version!	Bos taurus pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

PC ( XM_005226987.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227044.1 Bos taurus pyruvate carboxylase (PC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231848
Clone ID Related Accession (Same CDS sequence)	XM_005226987.4
Accession Version	XM_005226987.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226987.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC GTGGGTGCTC TCGGCCATGT GTGGCCTGGT GTTCCCCCCA ACTCCGCCCG 
CCCCCAGATA GTGCCTGCCT CCCAAGAGCC GACAAAACAC TGAGGATGCT GAAGTTTCAG 
ACTGTCCGGG GGAGCCTGAG GCTCCTGGCT ATCCGCCGGA CCTCCACCGC AACCGCTGCC 
TCTCCCAATG TCCGGCGCCT GGAGTACAAG CCCATCAAGA AGGTCATGGT GGCCAACAGA 
GGCGAGATCG CCATCCGTGT GTTCCGGGCC TGCACGGAGC TGGGCATCCG CACGGTGGCT 
GTCTACTCGG AGCAGGACAC GGGCCAAATG CACCGACAGA AAGCTGACGA AGCCTACCTC 
ATTGGCCGGG GTCTCGCCCC GGTGCAGGCC TATCTGCACA TTCCAGACAT CATTAAGGTC 
GCCAAGGAGA ACAACGTGGA CGCTGTGCAC CCGGGCTACG GGTTCCTGTC GGAGCGGGCC 
GACTTTGCCC AGGCTTGCCA GGATGCTGGG GTCCGGTTCA TTGGGCCAAG CCCCGAGGTG 
GTCCGCAAGA TGGGCGACAA GGTGGAGGCC CGGGCCATCG CCATCGCCGC AGGAGTGCCC 
GTGGTGCCCG GCACGGATGC CCCCATCACT TCCCTGCACG AGGCCCACGA GTTCTCCAAC 
ACCTACGGCT TCCCCATCAT CTTCAAGGCC GCCTACGGGG GCGGGGGCCG CGGCATGAGG 
GTCGTGCACA GCTACGAGGA GCTGGAGGAG AACTACACCC GGGCCTACTC GGAGGCTCTG 
GCCGCCTTCG GGAACGGGGC GCTGTTCGTG GAGAAGTTCA TCGAGAAGCC ACGCCACATC 
GAGGTGCAGA TCCTGGGGGA CCAGTACGGG AACATCCTTC ACCTCTACGA GCGGGACTGC 
TCCATCCAGC GGCGGCACCA GAAAGTGGTG GAGATCGCCC CGGCTGCCCA CTTGGACCCC 
CAGCTGCGCA CCCGGCTCAC TAGCGACTCC GTCAAGCTTG CAAAGCAGGT GGGCTACGAG 
AACGCGGGCA CCGTGGAGTT CCTGGTGGAC AGGCACGGCA AGCACTACTT CATCGAGGTC 
AACTCGCGCC TGCAAGTGGA GCACACGGTG ACCGAGGAGA TCACAGATGT GGACCTGGTC 
CACGCCCAGA TCCACGTGGC CGAGGGCCGG AGCCTGCCTG ACCTGGGCCT CCGGCAGGAG 
AACATCCGCA TCAACGGCTG TGCCATCCAG TGCCGGGTTA CCACCGAGGA CCCTGCGCGC 
AGCTTCCAGC CCGACACCGG CCGCATCGAG GTGTTCCGGA GCGGGGAGGG CATGGGCATC 
CGCCTGGACA ACGCCTCTGC CTTCCAAGGC GCCGTCATCT CCCCCCACTA CGACTCCCTG 
CTGGTCAAGG TCATCGCTCA CGGCAAGGAC CACCCCACGG CCGCCACCAA GATGAGCAGA 
GCCCTGGCCG AGTTCCGAGT CCGAGGCGTG AAGACCAACA TCCCCTTCCT GCAGAACGTG 
CTCAACAACC AGCAGTTCCT GGCAGGCACC GTGGACACGC AGTTCATCGA CGAGAACCCA 
GAGCTGTTCC AGCTGCGGCC CGCACAGAAC CGGGCCCAGA AGCTGCTGCA CTACCTCGGA 
CACGTCATGG TGAATGGCCC AACTACTCCG ATCCCCGTCA AGGCCAGCCC CAGCCCCACG 
GACCCCATCG TCCCTGTGGT GCCCATAGGC CCACCCCCAA CGGGTTTCAG AGACATCCTG 
CTACGGGAGG GGCCCGAGGG CTTTGCTCGA GCGGTGCGGA ACCATGAGGG GCTGCTGCTG 
ATGGACACGA CCTTCAGGGA CGCCCACCAG TCACTGCTGG CCACTCGAGT GCGCACCCAC 
GATCTCAAAA AGATCTCCCC CTATGTTGCC CACAGCTTCA ACAAACTCTT CAGCATAGAG 
AACTGGGGAG GAGCCACCTT TGACGTCGCC ATGCGCTTCC TGTACGAGTG CCCCTGGCGA 
CGGCTGCAGG AGCTCCGGGA GCTCGTCCCC AACATCCCAT TCCAGATGCT GCTGCGGGGG 
GCCAACGCCG TGGGCTACAC CAACTACCCC GACAATGTGG TCTTCAAGTT CTGCGAGGTG 
GCCAAGGAGA ATGGCATGGA CATCTTCCGG GTCTTTGACT CCCTCAACTA CCTGCCCAAC 
CTGCTGCTGG GCATGGAGGC GGCGGGCAGT GCTGGTGGCG TGGTGGAGGC CGCCATCTCC 
TACACGGGTG ACGTGTCCGA CCCCAGCCGC ACCAAGTACT CGCTGCAGTA CTACATGGGC 
TTGGCCGAGG AGCTGGTGCG AGCCGGCACC CACATCCTGT GCATCAAGGA CATGGCTGGG 
CTGCTGAAGC CTACGGCCTG CACCATGCTG GTCAGCTCCC TCCGGGACCG CTTCCCTGAC 
CTCCCGCTGC ACATCCACAC CCACGACACG TCAGGGGCGG GCGTGGCAGC CATGCTGGCC 
TGTGCTCACG CAGGGGCTGA CGTGGTGGAT GTGGCGGCTG ACTCCATGTC TGGAATGACA 
TCACAGCCCA GCATGGGGGC CCTGGTGGCC TGTACCCGAG GGACGCCCCT GGACACAGGC 
GTGCCCCTGG AGCGTGTGTT CGACTACAGC GAGTACTGGG AGGGGGCCCG GGGGCTGTAC 
GCGGCCTTTG ACTGCACGGC CACCATGAAG TCTGGCAACT CGGACGTGTA CGAGAACGAG 
ATCCCAGGGG GCCAGTACAC CAACCTGCAC TTCCAGGCGC ACAGCATGGG GCTCGGCTCC 
AAGTTCAAGG AGGTCAAGAA GGCCTACGTG GAGGCCAACC AGATGCTGGG CGACCTCATC 
AAGGTGACGC CCTCCTCCAA GATCGTGGGG GACCTGGCCC AGTTCATGGT GCAGAACGGG 
CTGACCCGAG CTGAGGCCGA AGCGCAGGCA GAGGAGCTGT CCTTCCCCCG CTCGGTGGTG 
GAGTTCCTGC AGGGCTACAT CGGCATCCCC CACGGGGGCT TCCCAGAGCC CCTTCGCTCC 
AAGGTGCTGA AAGACCTGCC GAGGGTGGAG GGCCGGCCAG GAGCCTCCCT CCCACCCCTG 
GACCTGCAGG CGCTGGAGAA GGAGCTGACG GAACGGCACG GGGAGGAGGT GACCCCGGAG 
GACGTGCTCT CAGCCGCCAT GTACCCCGAC GTCTTTGCCC ACTTCAAGGA CTTCACTGCC 
ACCTTCGGCC CCCTGGACAG CCTCAATACG CGCCTCTTCC TGCAGGGACC CAAGATTGCA 
GAGGAGTTTG AGGTGGAGCT GGAGCGAGGC AAGACGCTGC ACATCAAAGC CTTGGCCATA 
AGTGACCTCA ACCGGGCGGG CCAGAGGCAG GTCTTCTTCG AGCTCAACGG ACAGCTGCGG 
TCCATCCTGG TCAAGGACAC CCAGGCCATG AAGGAGATGC ACTTCCACCC CAAGGCCCTG 
AAGGACGTCA AGGGCCAGAT TGGGGCACCC ATGCCCGGGA AGGTGATAGA CATCAAGGTG 
GCAGCAGGGG CCAAGGTGAC CAAGGGCCAA CCCCTGTGTG TGCTCAGCGC CATGAAGATG 
GAGACTGTAG TGACCTCGCC CGTGGAGGGC ACTGTCCGCA AGGTCCACGT GACTAAGGAC 
ATGACGCTGG AGGGTGACGA CCTCATCCTG GAGATCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227044.1
CDS	69..3710
Translation	MAERGCSRPCVAWCSPQLRPPPDSACLPRADKTLRMLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226987.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226987.4

ORF Insert Sequence:

ATGGCAGAGC GTGGGTGCTC TCGGCCATGT GTGGCCTGGT GTTCCCCCCA ACTCCGCCCG 
CCCCCAGATA GTGCCTGCCT CCCAAGAGCC GACAAAACAC TGAGGATGCT GAAGTTTCAG 
ACTGTCCGGG GGAGCCTGAG GCTCCTGGCT ATCCGCCGGA CCTCCACCGC AACCGCTGCC 
TCTCCCAATG TCCGGCGCCT GGAGTACAAG CCCATCAAGA AGGTCATGGT GGCCAACAGA 
GGCGAGATCG CCATCCGTGT GTTCCGGGCC TGCACGGAGC TGGGCATCCG CACGGTGGCT 
GTCTACTCGG AGCAGGACAC GGGCCAAATG CACCGACAGA AAGCTGACGA AGCCTACCTC 
ATTGGCCGGG GTCTCGCCCC GGTGCAGGCC TATCTGCACA TTCCAGACAT CATTAAGGTC 
GCCAAGGAGA ACAACGTGGA CGCTGTGCAC CCGGGCTACG GGTTCCTGTC GGAGCGGGCC 
GACTTTGCCC AGGCTTGCCA GGATGCTGGG GTCCGGTTCA TTGGGCCAAG CCCCGAGGTG 
GTCCGCAAGA TGGGCGACAA GGTGGAGGCC CGGGCCATCG CCATCGCCGC AGGAGTGCCC 
GTGGTGCCCG GCACGGATGC CCCCATCACT TCCCTGCACG AGGCCCACGA GTTCTCCAAC 
ACCTACGGCT TCCCCATCAT CTTCAAGGCC GCCTACGGGG GCGGGGGCCG CGGCATGAGG 
GTCGTGCACA GCTACGAGGA GCTGGAGGAG AACTACACCC GGGCCTACTC GGAGGCTCTG 
GCCGCCTTCG GGAACGGGGC GCTGTTCGTG GAGAAGTTCA TCGAGAAGCC ACGCCACATC 
GAGGTGCAGA TCCTGGGGGA CCAGTACGGG AACATCCTTC ACCTCTACGA GCGGGACTGC 
TCCATCCAGC GGCGGCACCA GAAAGTGGTG GAGATCGCCC CGGCTGCCCA CTTGGACCCC 
CAGCTGCGCA CCCGGCTCAC TAGCGACTCC GTCAAGCTTG CAAAGCAGGT GGGCTACGAG 
AACGCGGGCA CCGTGGAGTT CCTGGTGGAC AGGCACGGCA AGCACTACTT CATCGAGGTC 
AACTCGCGCC TGCAAGTGGA GCACACGGTG ACCGAGGAGA TCACAGATGT GGACCTGGTC 
CACGCCCAGA TCCACGTGGC CGAGGGCCGG AGCCTGCCTG ACCTGGGCCT CCGGCAGGAG 
AACATCCGCA TCAACGGCTG TGCCATCCAG TGCCGGGTTA CCACCGAGGA CCCTGCGCGC 
AGCTTCCAGC CCGACACCGG CCGCATCGAG GTGTTCCGGA GCGGGGAGGG CATGGGCATC 
CGCCTGGACA ACGCCTCTGC CTTCCAAGGC GCCGTCATCT CCCCCCACTA CGACTCCCTG 
CTGGTCAAGG TCATCGCTCA CGGCAAGGAC CACCCCACGG CCGCCACCAA GATGAGCAGA 
GCCCTGGCCG AGTTCCGAGT CCGAGGCGTG AAGACCAACA TCCCCTTCCT GCAGAACGTG 
CTCAACAACC AGCAGTTCCT GGCAGGCACC GTGGACACGC AGTTCATCGA CGAGAACCCA 
GAGCTGTTCC AGCTGCGGCC CGCACAGAAC CGGGCCCAGA AGCTGCTGCA CTACCTCGGA 
CACGTCATGG TGAATGGCCC AACTACTCCG ATCCCCGTCA AGGCCAGCCC CAGCCCCACG 
GACCCCATCG TCCCTGTGGT GCCCATAGGC CCACCCCCAA CGGGTTTCAG AGACATCCTG 
CTACGGGAGG GGCCCGAGGG CTTTGCTCGA GCGGTGCGGA ACCATGAGGG GCTGCTGCTG 
ATGGACACGA CCTTCAGGGA CGCCCACCAG TCACTGCTGG CCACTCGAGT GCGCACCCAC 
GATCTCAAAA AGATCTCCCC CTATGTTGCC CACAGCTTCA ACAAACTCTT CAGCATAGAG 
AACTGGGGAG GAGCCACCTT TGACGTCGCC ATGCGCTTCC TGTACGAGTG CCCCTGGCGA 
CGGCTGCAGG AGCTCCGGGA GCTCGTCCCC AACATCCCAT TCCAGATGCT GCTGCGGGGG 
GCCAACGCCG TGGGCTACAC CAACTACCCC GACAATGTGG TCTTCAAGTT CTGCGAGGTG 
GCCAAGGAGA ATGGCATGGA CATCTTCCGG GTCTTTGACT CCCTCAACTA CCTGCCCAAC 
CTGCTGCTGG GCATGGAGGC GGCGGGCAGT GCTGGTGGCG TGGTGGAGGC CGCCATCTCC 
TACACGGGTG ACGTGTCCGA CCCCAGCCGC ACCAAGTACT CGCTGCAGTA CTACATGGGC 
TTGGCCGAGG AGCTGGTGCG AGCCGGCACC CACATCCTGT GCATCAAGGA CATGGCTGGG 
CTGCTGAAGC CTACGGCCTG CACCATGCTG GTCAGCTCCC TCCGGGACCG CTTCCCTGAC 
CTCCCGCTGC ACATCCACAC CCACGACACG TCAGGGGCGG GCGTGGCAGC CATGCTGGCC 
TGTGCTCACG CAGGGGCTGA CGTGGTGGAT GTGGCGGCTG ACTCCATGTC TGGAATGACA 
TCACAGCCCA GCATGGGGGC CCTGGTGGCC TGTACCCGAG GGACGCCCCT GGACACAGGC 
GTGCCCCTGG AGCGTGTGTT CGACTACAGC GAGTACTGGG AGGGGGCCCG GGGGCTGTAC 
GCGGCCTTTG ACTGCACGGC CACCATGAAG TCTGGCAACT CGGACGTGTA CGAGAACGAG 
ATCCCAGGGG GCCAGTACAC CAACCTGCAC TTCCAGGCGC ACAGCATGGG GCTCGGCTCC 
AAGTTCAAGG AGGTCAAGAA GGCCTACGTG GAGGCCAACC AGATGCTGGG CGACCTCATC 
AAGGTGACGC CCTCCTCCAA GATCGTGGGG GACCTGGCCC AGTTCATGGT GCAGAACGGG 
CTGACCCGAG CTGAGGCCGA AGCGCAGGCA GAGGAGCTGT CCTTCCCCCG CTCGGTGGTG 
GAGTTCCTGC AGGGCTACAT CGGCATCCCC CACGGGGGCT TCCCAGAGCC CCTTCGCTCC 
AAGGTGCTGA AAGACCTGCC GAGGGTGGAG GGCCGGCCAG GAGCCTCCCT CCCACCCCTG 
GACCTGCAGG CGCTGGAGAA GGAGCTGACG GAACGGCACG GGGAGGAGGT GACCCCGGAG 
GACGTGCTCT CAGCCGCCAT GTACCCCGAC GTCTTTGCCC ACTTCAAGGA CTTCACTGCC 
ACCTTCGGCC CCCTGGACAG CCTCAATACG CGCCTCTTCC TGCAGGGACC CAAGATTGCA 
GAGGAGTTTG AGGTGGAGCT GGAGCGAGGC AAGACGCTGC ACATCAAAGC CTTGGCCATA 
AGTGACCTCA ACCGGGCGGG CCAGAGGCAG GTCTTCTTCG AGCTCAACGG ACAGCTGCGG 
TCCATCCTGG TCAAGGACAC CCAGGCCATG AAGGAGATGC ACTTCCACCC CAAGGCCCTG 
AAGGACGTCA AGGGCCAGAT TGGGGCACCC ATGCCCGGGA AGGTGATAGA CATCAAGGTG 
GCAGCAGGGG CCAAGGTGAC CAAGGGCCAA CCCCTGTGTG TGCTCAGCGC CATGAAGATG 
GAGACTGTAG TGACCTCGCC CGTGGAGGGC ACTGTCCGCA AGGTCCACGT GACTAAGGAC 
ATGACGCTGG AGGGTGACGA CCTCATCCTG GAGATCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_024986991.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842759.1 Bos taurus pyruvate carboxylase (PC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_024986991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842759.1
CDS	145..3681
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_024986991.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986991.1

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226990.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227047.1 Bos taurus pyruvate carboxylase (PC), transcript variant X9, mRNA.

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CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226990.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226990.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227047.1
CDS	346..3882
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226990.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226990.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226994.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227051.1 Bos taurus pyruvate carboxylase (PC), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226994.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226994.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227051.1
CDS	140..3676
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226994.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226994.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226988.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227045.1 Bos taurus pyruvate carboxylase (PC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226988.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226988.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227045.1
CDS	364..3900
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226988.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226988.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226991.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227048.1 Bos taurus pyruvate carboxylase (PC), transcript variant X6, mRNA.

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CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226991.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226991.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227048.1
CDS	273..3809
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226991.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226991.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226989.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227046.1 Bos taurus pyruvate carboxylase (PC), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226989.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227046.1
CDS	550..4086
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226989.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226989.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226993.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227050.1 Bos taurus pyruvate carboxylase (PC), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226993.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227050.1
CDS	209..3745
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226993.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226993.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226992.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227049.1 Bos taurus pyruvate carboxylase (PC), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_005226992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226992.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227049.1
CDS	161..3697
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226992.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226992.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_015461252.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316738.1 Bos taurus pyruvate carboxylase (PC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_015461252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316738.1
CDS	458..3994
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_015461252.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461252.2

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_024986993.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842761.1 Bos taurus pyruvate carboxylase (PC), transcript variant X10, mRNA.

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CloneID	OBa231849
Clone ID Related Accession (Same CDS sequence)	XM_024986991.1 , XM_005226990.4 , XM_005226994.4 , XM_005226988.4 , XM_005226991.4 , XM_005226989.4 , XM_005226993.4 , XM_005226992.4 , XM_015461252.2 , XM_024986993.1
Accession Version	XM_024986993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842761.1
CDS	394..3930
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_024986993.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986993.1

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCCGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( NM_177946.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_808815.2 Bos taurus pyruvate carboxylase (PC), mRNA.

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CloneID	OBa01319
Clone ID Related Accession (Same CDS sequence)	NM_177946.4
Accession Version	NM_177946.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114135.1. On Aug 31, 2012 this sequence version replaced NM_177946.3. ##Evidence-Data-START## Transcript exon combination :: BC114135.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CGCGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCTCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GTTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808815.2
CDS	104..3640
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDATFRDAHQSLLATRVRTHDLKKISLYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_177946.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177946.4

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CGCGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCTCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GTTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226987.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227044.1 Bos taurus pyruvate carboxylase (PC), transcript variant X1, mRNA.

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CloneID	OBa34722
Clone ID Related Accession (Same CDS sequence)	XM_005226987.3
Accession Version	XM_005226987.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226987.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC GTGGGTGCTC TCGGCCATGT GTGGCCTGGT GTTCCCCCCA ACTCCGCCCG 
CCCCCAGATA GTGCCTGCCT CCCAAGAGCC GACAAAACAC TGAGGATGCT GAAGTTTCAG 
ACTGTCCGGG GGAGCCTGAG GCTCCTGGCT ATCCGCCGGA CCTCCACCGC AACCGCTGCC 
TCTCCCAATG TCCGGCGCCT GGAGTACAAG CCCATCAAGA AGGTCATGGT GGCCAACAGA 
GGCGAGATCG CCATCCGTGT GTTCCGGGCC TGCACGGAGC TGGGCATCCG CACGGTGGCT 
GTCTACTCGG AGCAGGACAC GGGCCAAATG CACCGACAGA AAGCTGACGA AGCCTACCTC 
ATTGGCCGGG GTCTCGCCCC GGTGCAGGCC TATCTGCACA TTCCAGACAT CATTAAGGTC 
GCCAAGGAGA ACAACGTGGA CGCTGTGCAC CCGGGCTACG GGTTCCTGTC GGAGCGGGCC 
GACTTTGCCC AGGCTTGCCA GGATGCTGGG GTCCGGTTCA TTGGGCCAAG CCCCGAGGTG 
GTCCGCAAGA TGGGCGACAA GGTGGAGGCC CGGGCCATCG CCATCGCCGC AGGAGTGCCC 
GTGGTGCCTG GCACGGATGC CCCCATCACT TCCCTGCACG AGGCCCACGA GTTCTCCAAC 
ACCTACGGCT TCCCCATCAT CTTCAAGGCC GCCTACGGGG GCGGGGGCCG CGGCATGAGG 
GTCGTGCACA GCTACGAGGA GCTGGAGGAG AACTACACCC GGGCCTACTC GGAGGCTCTG 
GCCGCCTTCG GGAACGGGGC GCTGTTCGTG GAGAAGTTCA TCGAGAAGCC ACGCCACATC 
GAGGTGCAGA TCCTGGGGGA CCAGTACGGG AACATCCTTC ACCTCTACGA GCGGGACTGC 
TCCATCCAGC GGCGGCACCA GAAAGTGGTG GAGATCGCCC CGGCTGCCCA CTTGGACCCC 
CAGCTGCGCA CCCGGCTCAC TAGCGACTCC GTCAAGCTTG CAAAGCAGGT GGGCTACGAG 
AACGCGGGCA CCGTGGAGTT CCTGGTGGAC AGGCACGGCA AGCACTACTT CATCGAGGTC 
AACTCGCGCC TGCAAGTGGA GCACACGGTG ACCGAGGAGA TCACAGATGT GGACCTGGTC 
CACGCCCAGA TCCACGTGGC CGAGGGCCGG AGCCTGCCTG ACCTGGGCCT CCGGCAGGAG 
AACATCCGCA TCAACGGCTG TGCCATCCAG TGCCGGGTTA CCACCGAGGA CCCTGCGCGC 
AGCTTCCAGC CCGACACCGG CCGCATCGAG GTGTTCCGGA GCGGGGAGGG CATGGGCATC 
CGCCTGGACA ACGCCTCTGC CTTCCAAGGC GCCGTCATCT CCCCCCACTA CGACTCCCTG 
CTGGTCAAGG TCATCGCTCA CGGCAAGGAC CACCCCACGG CCGCCACCAA GATGAGCAGA 
GCCCTGGCCG AGTTCCGAGT CCGAGGCGTG AAGACCAACA TCCCCTTCCT GCAGAACGTG 
CTCAACAACC AGCAGTTCCT GGCAGGCACC GTGGACACGC AGTTCATCGA CGAGAACCCA 
GAGCTGTTCC AGCTGCGGCC CGCACAGAAC CGGGCCCAGA AGCTGCTGCA CTACCTCGGA 
CACGTCATGG TGAATGGCCC AACTACTCCG ATCCCCGTCA AGGCCAGCCC CAGCCCCACG 
GACCCCATCG TCCCTGTGGT GCCCATAGGC CCACCCCCAA CGGGTTTCAG AGACATCCTG 
CTACGGGAGG GGCCCGAGGG CTTTGCTCGA GCGGTGCGGA ACCATGAGGG GCTGCTGCTG 
ATGGACACGA CCTTCAGGGA CGCCCACCAG TCACTGCTGG CCACTCGAGT GCGCACCCAC 
GATCTCAAAA AGATCTCCCC CTATGTTGCC CACAGCTTCA ACAAACTCTT CAGCATAGAG 
AACTGGGGAG GAGCCACCTT TGACGTCGCC ATGCGCTTCC TGTACGAGTG CCCCTGGCGA 
CGGCTGCAGG AGCTCCGGGA GCTCGTCCCC AACATCCCAT TCCAGATGCT GCTGCGGGGG 
GCCAACGCCG TGGGCTACAC CAACTACCCC GACAATGTGG TCTTCAAGTT CTGCGAGGTG 
GCCAAGGAGA ATGGCATGGA CATCTTCCGG GTCTTTGACT CCCTCAACTA CCTGCCCAAC 
CTGCTGCTGG GCATGGAGGC GGCGGGCAGT GCTGGTGGCG TGGTGGAGGC CGCCATCTCC 
TACACGGGTG ACGTGTCCGA CCCCAGCCGC ACCAAGTACT CGCTGCAGTA CTACATGGGC 
TTGGCCGAGG AGCTGGTGCG AGCCGGCACC CACATCCTGT GCATCAAGGA CATGGCTGGG 
CTGCTGAAGC CTACGGCCTG CACCATGCTG GTCAGCTCCC TCCGGGACCG CTTCCCTGAC 
CTCCCGCTGC ACATCCACAC CCACGACACG TCAGGGGCGG GCGTGGCAGC CATGCTGGCC 
TGTGCTCACG CAGGGGCTGA CGTGGTGGAT GTGGCGGCTG ACTCCATGTC TGGAATGACA 
TCACAGCCCA GCATGGGGGC CCTGGTGGCC TGTACCCGAG GGACGCCCCT GGACACAGGC 
GTGCCCCTGG AGCGTGTGTT CGACTACAGC GAGTACTGGG AGGGGGCCCG GGGGCTGTAC 
GCGGCCTTTG ACTGCACGGC CACCATGAAG TCTGGCAACT CGGACGTGTA CGAGAACGAG 
ATCCCAGGGG GCCAGTACAC CAACCTGCAC TTCCAGGCGC ACAGCATGGG GCTCGGCTCC 
AAGTTCAAGG AGGTCAAGAA GGCCTACGTG GAGGCCAACC AGATGCTGGG CGACCTCATC 
AAGGTGACGC CCTCCTCCAA GATCGTGGGG GACCTGGCCC AGTTCATGGT GCAGAACGGG 
CTGACCCGAG CTGAGGCCGA AGCGCAGGCA GAGGAGCTGT CCTTCCCCCG CTCGGTGGTG 
GAGTTCCTGC AGGGCTACAT CGGCATCCCC CACGGGGGCT TCCCAGAGCC CCTTCGCTCC 
AAGGTGCTGA AAGACCTGCC GAGGGTGGAG GGCCGGCCAG GAGCCTCCCT CCCACCCCTG 
GACCTGCAGG CGCTGGAGAA GGAGCTGACG GAACGGCACG GGGAGGAGGT GACCCCGGAG 
GACGTGCTCT CAGCCGCCAT GTACCCCGAC GTCTTTGCCC ACTTCAAGGA CTTCACTGCC 
ACCTTCGGCC CCCTGGACAG CCTCAATACG CGCCTCTTCC TGCAGGGACC CAAGATTGCA 
GAGGAGTTTG AGGTGGAGCT GGAGCGAGGC AAGACGCTGC ACATCAAAGC CTTGGCCATA 
AGTGACCTCA ACCGGGCGGG CCAGAGGCAG GTCTTCTTCG AGCTCAACGG ACAGCTGCGG 
TCCATCCTGG TCAAGGACAC CCAGGCCATG AAGGAGATGC ACTTCCACCC CAAGGCCCTG 
AAGGACGTCA AGGGCCAGAT TGGGGCACCC ATGCCCGGGA AGGTGATAGA CATCAAGGTG 
GCAGCAGGGG CCAAGGTGAC CAAGGGCCAA CCCCTGTGTG TGCTCAGCGC CATGAAGATG 
GAGACTGTAG TGACCTCGCC CGTGGAGGGC ACTGTCCGCA AGGTCCACGT GACTAAGGAC 
ATGACGCTGG AGGGTGACGA CCTCATCCTG GAGATCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227044.1
CDS	45..3686
Translation	MAERGCSRPCVAWCSPQLRPPPDSACLPRADKTLRMLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226987.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226987.3

ORF Insert Sequence:

ATGGCAGAGC GTGGGTGCTC TCGGCCATGT GTGGCCTGGT GTTCCCCCCA ACTCCGCCCG 
CCCCCAGATA GTGCCTGCCT CCCAAGAGCC GACAAAACAC TGAGGATGCT GAAGTTTCAG 
ACTGTCCGGG GGAGCCTGAG GCTCCTGGCT ATCCGCCGGA CCTCCACCGC AACCGCTGCC 
TCTCCCAATG TCCGGCGCCT GGAGTACAAG CCCATCAAGA AGGTCATGGT GGCCAACAGA 
GGCGAGATCG CCATCCGTGT GTTCCGGGCC TGCACGGAGC TGGGCATCCG CACGGTGGCT 
GTCTACTCGG AGCAGGACAC GGGCCAAATG CACCGACAGA AAGCTGACGA AGCCTACCTC 
ATTGGCCGGG GTCTCGCCCC GGTGCAGGCC TATCTGCACA TTCCAGACAT CATTAAGGTC 
GCCAAGGAGA ACAACGTGGA CGCTGTGCAC CCGGGCTACG GGTTCCTGTC GGAGCGGGCC 
GACTTTGCCC AGGCTTGCCA GGATGCTGGG GTCCGGTTCA TTGGGCCAAG CCCCGAGGTG 
GTCCGCAAGA TGGGCGACAA GGTGGAGGCC CGGGCCATCG CCATCGCCGC AGGAGTGCCC 
GTGGTGCCTG GCACGGATGC CCCCATCACT TCCCTGCACG AGGCCCACGA GTTCTCCAAC 
ACCTACGGCT TCCCCATCAT CTTCAAGGCC GCCTACGGGG GCGGGGGCCG CGGCATGAGG 
GTCGTGCACA GCTACGAGGA GCTGGAGGAG AACTACACCC GGGCCTACTC GGAGGCTCTG 
GCCGCCTTCG GGAACGGGGC GCTGTTCGTG GAGAAGTTCA TCGAGAAGCC ACGCCACATC 
GAGGTGCAGA TCCTGGGGGA CCAGTACGGG AACATCCTTC ACCTCTACGA GCGGGACTGC 
TCCATCCAGC GGCGGCACCA GAAAGTGGTG GAGATCGCCC CGGCTGCCCA CTTGGACCCC 
CAGCTGCGCA CCCGGCTCAC TAGCGACTCC GTCAAGCTTG CAAAGCAGGT GGGCTACGAG 
AACGCGGGCA CCGTGGAGTT CCTGGTGGAC AGGCACGGCA AGCACTACTT CATCGAGGTC 
AACTCGCGCC TGCAAGTGGA GCACACGGTG ACCGAGGAGA TCACAGATGT GGACCTGGTC 
CACGCCCAGA TCCACGTGGC CGAGGGCCGG AGCCTGCCTG ACCTGGGCCT CCGGCAGGAG 
AACATCCGCA TCAACGGCTG TGCCATCCAG TGCCGGGTTA CCACCGAGGA CCCTGCGCGC 
AGCTTCCAGC CCGACACCGG CCGCATCGAG GTGTTCCGGA GCGGGGAGGG CATGGGCATC 
CGCCTGGACA ACGCCTCTGC CTTCCAAGGC GCCGTCATCT CCCCCCACTA CGACTCCCTG 
CTGGTCAAGG TCATCGCTCA CGGCAAGGAC CACCCCACGG CCGCCACCAA GATGAGCAGA 
GCCCTGGCCG AGTTCCGAGT CCGAGGCGTG AAGACCAACA TCCCCTTCCT GCAGAACGTG 
CTCAACAACC AGCAGTTCCT GGCAGGCACC GTGGACACGC AGTTCATCGA CGAGAACCCA 
GAGCTGTTCC AGCTGCGGCC CGCACAGAAC CGGGCCCAGA AGCTGCTGCA CTACCTCGGA 
CACGTCATGG TGAATGGCCC AACTACTCCG ATCCCCGTCA AGGCCAGCCC CAGCCCCACG 
GACCCCATCG TCCCTGTGGT GCCCATAGGC CCACCCCCAA CGGGTTTCAG AGACATCCTG 
CTACGGGAGG GGCCCGAGGG CTTTGCTCGA GCGGTGCGGA ACCATGAGGG GCTGCTGCTG 
ATGGACACGA CCTTCAGGGA CGCCCACCAG TCACTGCTGG CCACTCGAGT GCGCACCCAC 
GATCTCAAAA AGATCTCCCC CTATGTTGCC CACAGCTTCA ACAAACTCTT CAGCATAGAG 
AACTGGGGAG GAGCCACCTT TGACGTCGCC ATGCGCTTCC TGTACGAGTG CCCCTGGCGA 
CGGCTGCAGG AGCTCCGGGA GCTCGTCCCC AACATCCCAT TCCAGATGCT GCTGCGGGGG 
GCCAACGCCG TGGGCTACAC CAACTACCCC GACAATGTGG TCTTCAAGTT CTGCGAGGTG 
GCCAAGGAGA ATGGCATGGA CATCTTCCGG GTCTTTGACT CCCTCAACTA CCTGCCCAAC 
CTGCTGCTGG GCATGGAGGC GGCGGGCAGT GCTGGTGGCG TGGTGGAGGC CGCCATCTCC 
TACACGGGTG ACGTGTCCGA CCCCAGCCGC ACCAAGTACT CGCTGCAGTA CTACATGGGC 
TTGGCCGAGG AGCTGGTGCG AGCCGGCACC CACATCCTGT GCATCAAGGA CATGGCTGGG 
CTGCTGAAGC CTACGGCCTG CACCATGCTG GTCAGCTCCC TCCGGGACCG CTTCCCTGAC 
CTCCCGCTGC ACATCCACAC CCACGACACG TCAGGGGCGG GCGTGGCAGC CATGCTGGCC 
TGTGCTCACG CAGGGGCTGA CGTGGTGGAT GTGGCGGCTG ACTCCATGTC TGGAATGACA 
TCACAGCCCA GCATGGGGGC CCTGGTGGCC TGTACCCGAG GGACGCCCCT GGACACAGGC 
GTGCCCCTGG AGCGTGTGTT CGACTACAGC GAGTACTGGG AGGGGGCCCG GGGGCTGTAC 
GCGGCCTTTG ACTGCACGGC CACCATGAAG TCTGGCAACT CGGACGTGTA CGAGAACGAG 
ATCCCAGGGG GCCAGTACAC CAACCTGCAC TTCCAGGCGC ACAGCATGGG GCTCGGCTCC 
AAGTTCAAGG AGGTCAAGAA GGCCTACGTG GAGGCCAACC AGATGCTGGG CGACCTCATC 
AAGGTGACGC CCTCCTCCAA GATCGTGGGG GACCTGGCCC AGTTCATGGT GCAGAACGGG 
CTGACCCGAG CTGAGGCCGA AGCGCAGGCA GAGGAGCTGT CCTTCCCCCG CTCGGTGGTG 
GAGTTCCTGC AGGGCTACAT CGGCATCCCC CACGGGGGCT TCCCAGAGCC CCTTCGCTCC 
AAGGTGCTGA AAGACCTGCC GAGGGTGGAG GGCCGGCCAG GAGCCTCCCT CCCACCCCTG 
GACCTGCAGG CGCTGGAGAA GGAGCTGACG GAACGGCACG GGGAGGAGGT GACCCCGGAG 
GACGTGCTCT CAGCCGCCAT GTACCCCGAC GTCTTTGCCC ACTTCAAGGA CTTCACTGCC 
ACCTTCGGCC CCCTGGACAG CCTCAATACG CGCCTCTTCC TGCAGGGACC CAAGATTGCA 
GAGGAGTTTG AGGTGGAGCT GGAGCGAGGC AAGACGCTGC ACATCAAAGC CTTGGCCATA 
AGTGACCTCA ACCGGGCGGG CCAGAGGCAG GTCTTCTTCG AGCTCAACGG ACAGCTGCGG 
TCCATCCTGG TCAAGGACAC CCAGGCCATG AAGGAGATGC ACTTCCACCC CAAGGCCCTG 
AAGGACGTCA AGGGCCAGAT TGGGGCACCC ATGCCCGGGA AGGTGATAGA CATCAAGGTG 
GCAGCAGGGG CCAAGGTGAC CAAGGGCCAA CCCCTGTGTG TGCTCAGCGC CATGAAGATG 
GAGACTGTAG TGACCTCGCC CGTGGAGGGC ACTGTCCGCA AGGTCCACGT GACTAAGGAC 
ATGACGCTGG AGGGTGACGA CCTCATCCTG GAGATCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226988.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227045.1 Bos taurus pyruvate carboxylase (PC), transcript variant X2, mRNA.

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CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227045.1
CDS	211..3747
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226988.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226988.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226992.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227049.1 Bos taurus pyruvate carboxylase (PC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227049.1
CDS	120..3656
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226992.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226992.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226991.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227048.1 Bos taurus pyruvate carboxylase (PC), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227048.1
CDS	256..3792
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226991.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226991.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226993.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227050.1 Bos taurus pyruvate carboxylase (PC), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227050.1
CDS	175..3711
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226993.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226993.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226994.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227051.1 Bos taurus pyruvate carboxylase (PC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226994.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227051.1
CDS	118..3654
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226994.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226994.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226990.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227047.1 Bos taurus pyruvate carboxylase (PC), transcript variant X5, mRNA.

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CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227047.1
CDS	305..3841
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226990.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226990.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005226989.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227046.1 Bos taurus pyruvate carboxylase (PC), transcript variant X4, mRNA.

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CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_005226989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227046.1
CDS	396..3932
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005226989.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226989.3

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_015461252.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316738.1 Bos taurus pyruvate carboxylase (PC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_015461252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316738.1
CDS	441..3977
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_015461252.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461252.1

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_015461253.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316739.1 Bos taurus pyruvate carboxylase (PC), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa34723
Clone ID Related Accession (Same CDS sequence)	XM_005226988.3 , XM_005226992.3 , XM_005226991.3 , XM_005226993.3 , XM_005226994.3 , XM_005226990.3 , XM_005226989.3 , XM_015461252.1 , XM_015461253.1
Accession Version	XM_015461253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316739.1
CDS	296..3832
Translation	MLKFQTVRGSLRLLAIRRTSTATAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPIVPVVPIGPPPTGFRDILLREGPEGFARAVRNHEGLLLMDTTFRDAHQSLLATRVRTHDLKKISPYVAHSFNKLFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAAGSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVTKGQPLCVLSAMKMETVVTSPVEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_015461253.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pyruvate carboxylase (PC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461253.1

ORF Insert Sequence:

ATGCTGAAGT TTCAGACTGT CCGGGGGAGC CTGAGGCTCC TGGCTATCCG CCGGACCTCC 
ACCGCAACCG CTGCCTCTCC CAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAGGTC 
ATGGTGGCCA ACAGAGGCGA GATCGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACGG TGGCTGTCTA CTCGGAGCAG GACACGGGCC AAATGCACCG ACAGAAAGCT 
GACGAAGCCT ACCTCATTGG CCGGGGTCTC GCCCCGGTGC AGGCCTATCT GCACATTCCA 
GACATCATTA AGGTCGCCAA GGAGAACAAC GTGGACGCTG TGCACCCGGG CTACGGGTTC 
CTGTCGGAGC GGGCCGACTT TGCCCAGGCT TGCCAGGATG CTGGGGTCCG GTTCATTGGG 
CCAAGCCCCG AGGTGGTCCG CAAGATGGGC GACAAGGTGG AGGCCCGGGC CATCGCCATC 
GCCGCAGGAG TGCCCGTGGT GCCTGGCACG GATGCCCCCA TCACTTCCCT GCACGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCCGCCTA CGGGGGCGGG 
GGCCGCGGCA TGAGGGTCGT GCACAGCTAC GAGGAGCTGG AGGAGAACTA CACCCGGGCC 
TACTCGGAGG CTCTGGCCGC CTTCGGGAAC GGGGCGCTGT TCGTGGAGAA GTTCATCGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGGGACCAGT ACGGGAACAT CCTTCACCTC 
TACGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAAG TGGTGGAGAT CGCCCCGGCT 
GCCCACTTGG ACCCCCAGCT GCGCACCCGG CTCACTAGCG ACTCCGTCAA GCTTGCAAAG 
CAGGTGGGCT ACGAGAACGC GGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC GCGCCTGCAA GTGGAGCACA CGGTGACCGA GGAGATCACA 
GATGTGGACC TGGTCCACGC CCAGATCCAC GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTCCGGC AGGAGAACAT CCGCATCAAC GGCTGTGCCA TCCAGTGCCG GGTTACCACC 
GAGGACCCTG CGCGCAGCTT CCAGCCCGAC ACCGGCCGCA TCGAGGTGTT CCGGAGCGGG 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCCTTCC AAGGCGCCGT CATCTCCCCC 
CACTACGACT CCCTGCTGGT CAAGGTCATC GCTCACGGCA AGGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGAGTCCGAG GCGTGAAGAC CAACATCCCC 
TTCCTGCAGA ACGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACGCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCCGCAC AGAACCGGGC CCAGAAGCTG 
CTGCACTACC TCGGACACGT CATGGTGAAT GGCCCAACTA CTCCGATCCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CATCGTCCCT GTGGTGCCCA TAGGCCCACC CCCAACGGGT 
TTCAGAGACA TCCTGCTACG GGAGGGGCCC GAGGGCTTTG CTCGAGCGGT GCGGAACCAT 
GAGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGAGTGCGCA CCCACGATCT CAAAAAGATC TCCCCCTATG TTGCCCACAG CTTCAACAAA 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACCTTTGACG TCGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGACGGCT GCAGGAGCTC CGGGAGCTCG TCCCCAACAT CCCATTCCAG 
ATGCTGCTGC GGGGGGCCAA CGCCGTGGGC TACACCAACT ACCCCGACAA TGTGGTCTTC 
AAGTTCTGCG AGGTGGCCAA GGAGAATGGC ATGGACATCT TCCGGGTCTT TGACTCCCTC 
AACTACCTGC CCAACCTGCT GCTGGGCATG GAGGCGGCGG GCAGTGCTGG TGGCGTGGTG 
GAGGCCGCCA TCTCCTACAC GGGTGACGTG TCCGACCCCA GCCGCACCAA GTACTCGCTG 
CAGTACTACA TGGGCTTGGC CGAGGAGCTG GTGCGAGCCG GCACCCACAT CCTGTGCATC 
AAGGACATGG CTGGGCTGCT GAAGCCTACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CTGACCTCCC GCTGCACATC CACACCCACG ACACGTCAGG GGCGGGCGTG 
GCAGCCATGC TGGCCTGTGC TCACGCAGGG GCTGACGTGG TGGATGTGGC GGCTGACTCC 
ATGTCTGGAA TGACATCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CCGAGGGACG 
CCCCTGGACA CAGGCGTGCC CCTGGAGCGT GTGTTCGACT ACAGCGAGTA CTGGGAGGGG 
GCCCGGGGGC TGTACGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTACGAGA ACGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCGCACAGC 
ATGGGGCTCG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ACGTGGAGGC CAACCAGATG 
CTGGGCGACC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ACGGGCTGAC CCGAGCTGAG GCCGAAGCGC AGGCAGAGGA GCTGTCCTTC 
CCCCGCTCGG TGGTGGAGTT CCTGCAGGGC TACATCGGCA TCCCCCACGG GGGCTTCCCA 
GAGCCCCTTC GCTCCAAGGT GCTGAAAGAC CTGCCGAGGG TGGAGGGCCG GCCAGGAGCC 
TCCCTCCCAC CCCTGGACCT GCAGGCGCTG GAGAAGGAGC TGACGGAACG GCACGGGGAG 
GAGGTGACCC CGGAGGACGT GCTCTCAGCC GCCATGTACC CCGACGTCTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT CGGCCCCCTG GACAGCCTCA ATACGCGCCT CTTCCTGCAG 
GGACCCAAGA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GAGGCAAGAC GCTGCACATC 
AAAGCCTTGG CCATAAGTGA CCTCAACCGG GCGGGCCAGA GGCAGGTCTT CTTCGAGCTC 
AACGGACAGC TGCGGTCCAT CCTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTGAAGGA CGTCAAGGGC CAGATTGGGG CACCCATGCC CGGGAAGGTG 
ATAGACATCA AGGTGGCAGC AGGGGCCAAG GTGACCAAGG GCCAACCCCT GTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTAGTGACC TCGCCCGTGG AGGGCACTGT CCGCAAGGTC 
CACGTGACTA AGGACATGAC GCTGGAGGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PC
Entrez Gene ID	338471
Full Name	pyruvate carboxylase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PC cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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