MYLK cDNA ORF clone, Bos taurus(cattle)

The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218620 XM_024994569.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OBa218619 XM_024994520.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa261971 NM_176636.3
Latest version!
Bos taurus myosin light chain kinase (MYLK), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa218618 NM_001372254.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218618 XM_024994475.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218615 XM_024994340.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa218616 XM_024994382.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa218617 XM_024994421.1
Latest version!
Bos taurus myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa43045 NM_176636.2
Latest version!
Bos taurus myosin light chain kinase (MYLK), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218620
    Clone ID Related Accession (Same CDS sequence) XM_024994569.1
    Accession Version XM_024994569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT GGAGGCTGTC
    GCTGAGGAGA AGCCCCATGT AAAACCCTAC TTTTCCAAGA CCATTCGTGA CTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCGGA CCCTGAAGTC
    GTCTGGTTCA AAGATGACCA ATCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGT GATGTCTGCG GGGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATCGTGGAA
    ACCATGGAGG AAGGAGAAGG AGAAGGGGGA GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850337.1
    CDS130..600
    Translation

    Target ORF information:

    RefSeq Version XM_024994569.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT GGAGGCTGTC
    GCTGAGGAGA AGCCCCATGT AAAACCCTAC TTTTCCAAGA CCATTCGTGA CTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCGGA CCCTGAAGTC
    GTCTGGTTCA AAGATGACCA ATCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGT GATGTCTGCG GGGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATCGTGGAA
    ACCATGGAGG AAGGAGAAGG AGAAGGGGGA GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218619
    Clone ID Related Accession (Same CDS sequence) XM_024994520.1
    Accession Version XM_024994520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC GCCTCAGATC
    ATCCAGTTTC CTGAGGACCA GAAGGTGCGA GCAGGAGAGT CTGTGGAGCT CTTTGGTAAG
    GTGGCCGGCA CCCAGCCCAT CACCTGCACC TGGATGAAGT TCCGAAAGCA GATCCAGGAC
    AGCGAGCACA TCAAGGTGGA GAACAGCGAG CAGGGCAGCA AGCTCACCAT CCGGGCGGCA
    CGCCAGGAGC ACTGTGGCTG CTACACCCTG CTGGTGGAGA ACAAACTGGG CAGCAGGCAG
    GCCCAAGTCA ACCTCACCGT GGTGGACAAG CCAGACCCCC CAGCCGGTAC ACCTTGTGCC
    TCTGACATCC GAAGCTCCTC ACTGACCTTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGCGCCGTAC AGTCATACAG TGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC TTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCGAGAATAT
    AAGTTCCGTG TGCGTGCAAT CAATGTCTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCCT TGGGAGAGAA ACCTGAAGAA GAGCCGAAGG ACGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCTGA GGTCGACTAC CGGACCGTCA CGGTCAATAC TGAGCAGAAA
    GTGTCCGACT TCTACGACAT CGAAGAGAGA CTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TGGAAAAATC TGGGCAGGGA AATTCTTCAA GGCGTATTCG
    GCCAAGGAGA AAGAGAACAT CCGGCAGGAG ATCAGCATCA TGAACTGCCT GCACCACCCC
    AAGCTGGTCC AGTGTGTGGA CGCCTTCGAA GAGAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCCG GGGGTGAGCT GTTCGAGCGC ATCATTGATG AGGACTTTGA GCTGACGGAG
    CGGGAGTGCA TCAAGTACAT GAAGCAGATC TCAGAGGGGG TGGAGTACAT CCACAAGCAG
    GGCATCGTCC ACCTGGACCT CAAGCCCGAG AACATCATGT GTGTCAACAA GACAGGCACC
    AGGATCAAGC TCATCGACTT CGGTCTGGCC AGGAGGTTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCGGA GTTCGTCGCA CCCGAAGTGA TCAACTATGA GCCCATTGGC
    TATGCCACGG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTGGTCAG TGGACTGTCC
    CCCTTCATGG GTGACAATGA CAACGAAACC TTAGCCAACG TTACCTCAGC CACCTGGGAC
    TTTGACGACG AGGCCTTCGA CGAGATCTCC GATGACGCCA AGGATTTTAT CAGCAACTTG
    CTGAAGAAGG ATATGAAAAA CCGCCTGAAC TGCACCCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGACA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATCGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GAAGATGTCT CCCAAGCTTT CCTGGAGGCT
    GTCGCTGAGG AGAAGCCCCA TGTAAAACCC TACTTTTCCA AGACCATTCG TGACTTAGAA
    GTTGTGGAGG GAAGTGCTGC TAGATTTGAC TGCAAGATTG AAGGATACCC GGACCCTGAA
    GTCGTCTGGT TCAAAGATGA CCAATCAATC CGGGAGTCCC GCCACTTCCA GATAGACTAC
    GATGAGGATG GGAACTGCTC TTTAATCATT AGTGATGTCT GCGGGGATGA TGATGCCAAG
    TACACCTGCA AGGCTGTCAA CAGTCTTGGA GAAGCCACCT GCACAGCAGA GCTCATCGTG
    GAAACCATGG AGGAAGGAGA AGGAGAAGGG GGAGAAGAGG AGGAGGAAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850288.1
    CDS181..2334
    Translation

    Target ORF information:

    RefSeq Version XM_024994520.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC GCCTCAGATC
    ATCCAGTTTC CTGAGGACCA GAAGGTGCGA GCAGGAGAGT CTGTGGAGCT CTTTGGTAAG
    GTGGCCGGCA CCCAGCCCAT CACCTGCACC TGGATGAAGT TCCGAAAGCA GATCCAGGAC
    AGCGAGCACA TCAAGGTGGA GAACAGCGAG CAGGGCAGCA AGCTCACCAT CCGGGCGGCA
    CGCCAGGAGC ACTGTGGCTG CTACACCCTG CTGGTGGAGA ACAAACTGGG CAGCAGGCAG
    GCCCAAGTCA ACCTCACCGT GGTGGACAAG CCAGACCCCC CAGCCGGTAC ACCTTGTGCC
    TCTGACATCC GAAGCTCCTC ACTGACCTTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGCGCCGTAC AGTCATACAG TGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC TTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCGAGAATAT
    AAGTTCCGTG TGCGTGCAAT CAATGTCTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCCT TGGGAGAGAA ACCTGAAGAA GAGCCGAAGG ACGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCTGA GGTCGACTAC CGGACCGTCA CGGTCAATAC TGAGCAGAAA
    GTGTCCGACT TCTACGACAT CGAAGAGAGA CTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TGGAAAAATC TGGGCAGGGA AATTCTTCAA GGCGTATTCG
    GCCAAGGAGA AAGAGAACAT CCGGCAGGAG ATCAGCATCA TGAACTGCCT GCACCACCCC
    AAGCTGGTCC AGTGTGTGGA CGCCTTCGAA GAGAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCCG GGGGTGAGCT GTTCGAGCGC ATCATTGATG AGGACTTTGA GCTGACGGAG
    CGGGAGTGCA TCAAGTACAT GAAGCAGATC TCAGAGGGGG TGGAGTACAT CCACAAGCAG
    GGCATCGTCC ACCTGGACCT CAAGCCCGAG AACATCATGT GTGTCAACAA GACAGGCACC
    AGGATCAAGC TCATCGACTT CGGTCTGGCC AGGAGGTTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCGGA GTTCGTCGCA CCCGAAGTGA TCAACTATGA GCCCATTGGC
    TATGCCACGG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTGGTCAG TGGACTGTCC
    CCCTTCATGG GTGACAATGA CAACGAAACC TTAGCCAACG TTACCTCAGC CACCTGGGAC
    TTTGACGACG AGGCCTTCGA CGAGATCTCC GATGACGCCA AGGATTTTAT CAGCAACTTG
    CTGAAGAAGG ATATGAAAAA CCGCCTGAAC TGCACCCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGACA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATCGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GAAGATGTCT CCCAAGCTTT CCTGGAGGCT
    GTCGCTGAGG AGAAGCCCCA TGTAAAACCC TACTTTTCCA AGACCATTCG TGACTTAGAA
    GTTGTGGAGG GAAGTGCTGC TAGATTTGAC TGCAAGATTG AAGGATACCC GGACCCTGAA
    GTCGTCTGGT TCAAAGATGA CCAATCAATC CGGGAGTCCC GCCACTTCCA GATAGACTAC
    GATGAGGATG GGAACTGCTC TTTAATCATT AGTGATGTCT GCGGGGATGA TGATGCCAAG
    TACACCTGCA AGGCTGTCAA CAGTCTTGGA GAAGCCACCT GCACAGCAGA GCTCATCGTG
    GAAACCATGG AGGAAGGAGA AGGAGAAGGG GGAGAAGAGG AGGAGGAAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa261971
    Clone ID Related Accession (Same CDS sequence) NM_176636.3
    Accession Version NM_176636.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Aug 2, 2019 this sequence version replaced NM_176636.2. ##Evidence-Data-START## Transcript exon combination :: S57131.1, SRR5721697.3806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG 
    AGGAAAGTGC ATGGCCCCCA GCAGGTGGAC TTCCGCTCTG TCCTAGCCAA GAAGGGGACT
    CCCAAGACCC CTGTGCCTGA GAAGGTACCC CCTCCAAAAC CGGCCACGCC AGATTTTCGC
    TCAGTGTTGG GCAGTAAGAA AAAATTACCA ACAGAGAATG GTAGCAATAA CACTGAGGCC
    TTGAATGCCA AGGCAGCAGA AGGTCTCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGGTTC
    CTGAAACCCG TGGGCAACGC CAAGCTGGCT GATACCCCCA AACCCTTGAG CAGCACCAAG
    CCAGCAGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAACGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAACATCAAG
    CCAACTGAGA CCCCCAAACC CTTGGGCAGC ACCAAGCCAG CAGAAACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA GACCCCAAAA CCCTTGGGTA ATGTCAAGCC AGCCGAGACC
    CCCAAACCCT TGGGTAATGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCTAAACC TGTGAGCAAT GCCAAGCCAG CCGAAACCCT CAAACCTGTG
    GGCAACGCCA AGCCAGCTGA GACCCCCAAA CCCTTGAGTA ACGTCAAGCC AGCTGAAACC
    CCCAAACTTG TGGGCAATGC CAAGCCAGCT GAGACCTCTA AACCCTTGGA TAACGCCAAG
    CCAGCTGAGG CCCCCAAACC CTTGGGCAAT GCCAAGCCGG CTGAAATCCC AAAACCCACT
    GGGAAAGAAG AACTTAAGAA GGAGATTAAG AATGATGTGA ACTGCAAGAA GGGCCATGCA
    GGGGCCACAG ATAGTGAAAA GAGACCAGAG AGCCGAGGGA CAGCCCCGAC CTTCGAGGAG
    AAACTACAAG ATCTCCATGT GGCAGAGGGT CAGAAGCTGC TGCTCCAGTG CCGGGTGTCC
    TCTGACCCCC CAGCCACCAT CACTTGGACA CTGAATGGAA AGACGCTCAA GACCACCAAG
    TTCATCGTCC TCTCCCAAGA AGGCTCCTTG TGCTCCGTCT CCATCGAGAA GGCACTGCCC
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAACAGTG CCGGCCAGGC TGAGTCCTCC
    TGCCAAGTCA CCGTGGACGT TCCAGATGCC CCCACCAGTG AAAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC GCCTCAGATC
    ATCCAGTTTC CTGAGGACCA GAAGGTGCGA GCAGGAGAGT CTGTGGAGCT CTTTGGTAAG
    GTGGCCGGCA CCCAGCCCAT CACCTGCACC TGGATGAAGT TCCGAAAGCA GATCCAGGAC
    AGCGAGCACA TCAAGGTGGA GAACAGCGAG CAGGGCAGCA AGCTCACCAT CCGGGCGGCA
    CGCCAGGAGC ACTGTGGCTG CTACACCCTG CTGGTGGAGA ACAAACTGGG CAGCAGGCAG
    GCCCAAGTCA ACCTCACCGT GGTGGACAAG CCAGACCCCC CAGCCGGTAC ACCTTGTGCC
    TCTGACATCC GAAGCTCCTC ACTGACCTTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGCGCCGTAC AGTCATACAG TGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC TTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCGAGAATAT
    AAGTTCCGTG TGCGTGCAAT CAATGTCTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCCT TGGGAGAGAA ACCTGAAGAA GAGCCGAAGG ACGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCTGA GGTCGACTAC CGGACCGTCA CGGTCAATAC TGAGCAGAAA
    GTGTCCGACT TCTACGACAT CGAAGAGAGA CTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TGGAAAAATC TGGGCAGGGA AATTCTTCAA GGCGTATTCG
    GCCAAGGAGA AAGAGAACAT CCGGCAGGAG ATCAGCATCA TGAACTGCCT GCACCACCCC
    AAGCTGGTCC AGTGTGTGGA CGCCTTCGAA GAGAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCCG GGGGTGAGCT GTTCGAGCGC ATCATTGATG AGGACTTTGA GCTGACGGAG
    CGGGAGTGCA TCAAGTACAT GAAGCAGATC TCAGAGGGGG TGGAGTACAT CCACAAGCAG
    GGCATCGTCC ACCTGGACCT CAAGCCCGAG AACATCATGT GTGTCAACAA GACAGGCACC
    AGGATCAAGC TCATCGACTT CGGTCTGGCC AGGAGGTTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCGGA GTTCGTCGCA CCCGAAGTGA TCAACTATGA GCCCATTGGC
    TATGCCACGG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTGGTCAG TGGACTGTCC
    CCCTTCATGG GTGACAATGA CAACGAAACC TTAGCCAACG TTACCTCAGC CACCTGGGAC
    TTTGACGACG AGGCCTTCGA CGAGATCTCC GATGACGCCA AGGATTTTAT CAGCAACTTG
    CTGAAGAAGG ATATGAAAAA CCGCCTGAAC TGCACCCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGACA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATCGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GATGTCTCCC AAGCTTTCCT GGAGGCTGTC
    GCTGAGGAGA AGCCCCATGT AAAACCCTAC TTTTCCAAGA CCATTCGTGA CTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCGGA CCCTGAAGTC
    GTCTGGTTCA AAGATGACCA ATCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGT GATGTCTGCG GGGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATCGTGGAA
    ACCATGGAGG AAGGAGAAGG AGAAGGGGGA GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788809.1
    CDS381..3911
    Translation

    Target ORF information:

    RefSeq Version NM_176636.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176636.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG 
    AGGAAAGTGC ATGGCCCCCA GCAGGTGGAC TTCCGCTCTG TCCTAGCCAA GAAGGGGACT
    CCCAAGACCC CTGTGCCTGA GAAGGTACCC CCTCCAAAAC CGGCCACGCC AGATTTTCGC
    TCAGTGTTGG GCAGTAAGAA AAAATTACCA ACAGAGAATG GTAGCAATAA CACTGAGGCC
    TTGAATGCCA AGGCAGCAGA AGGTCTCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGGTTC
    CTGAAACCCG TGGGCAACGC CAAGCTGGCT GATACCCCCA AACCCTTGAG CAGCACCAAG
    CCAGCAGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAACGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAACATCAAG
    CCAACTGAGA CCCCCAAACC CTTGGGCAGC ACCAAGCCAG CAGAAACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA GACCCCAAAA CCCTTGGGTA ATGTCAAGCC AGCCGAGACC
    CCCAAACCCT TGGGTAATGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCTAAACC TGTGAGCAAT GCCAAGCCAG CCGAAACCCT CAAACCTGTG
    GGCAACGCCA AGCCAGCTGA GACCCCCAAA CCCTTGAGTA ACGTCAAGCC AGCTGAAACC
    CCCAAACTTG TGGGCAATGC CAAGCCAGCT GAGACCTCTA AACCCTTGGA TAACGCCAAG
    CCAGCTGAGG CCCCCAAACC CTTGGGCAAT GCCAAGCCGG CTGAAATCCC AAAACCCACT
    GGGAAAGAAG AACTTAAGAA GGAGATTAAG AATGATGTGA ACTGCAAGAA GGGCCATGCA
    GGGGCCACAG ATAGTGAAAA GAGACCAGAG AGCCGAGGGA CAGCCCCGAC CTTCGAGGAG
    AAACTACAAG ATCTCCATGT GGCAGAGGGT CAGAAGCTGC TGCTCCAGTG CCGGGTGTCC
    TCTGACCCCC CAGCCACCAT CACTTGGACA CTGAATGGAA AGACGCTCAA GACCACCAAG
    TTCATCGTCC TCTCCCAAGA AGGCTCCTTG TGCTCCGTCT CCATCGAGAA GGCACTGCCC
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAACAGTG CCGGCCAGGC TGAGTCCTCC
    TGCCAAGTCA CCGTGGACGT TCCAGATGCC CCCACCAGTG AAAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC GCCTCAGATC
    ATCCAGTTTC CTGAGGACCA GAAGGTGCGA GCAGGAGAGT CTGTGGAGCT CTTTGGTAAG
    GTGGCCGGCA CCCAGCCCAT CACCTGCACC TGGATGAAGT TCCGAAAGCA GATCCAGGAC
    AGCGAGCACA TCAAGGTGGA GAACAGCGAG CAGGGCAGCA AGCTCACCAT CCGGGCGGCA
    CGCCAGGAGC ACTGTGGCTG CTACACCCTG CTGGTGGAGA ACAAACTGGG CAGCAGGCAG
    GCCCAAGTCA ACCTCACCGT GGTGGACAAG CCAGACCCCC CAGCCGGTAC ACCTTGTGCC
    TCTGACATCC GAAGCTCCTC ACTGACCTTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGCGCCGTAC AGTCATACAG TGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC TTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCGAGAATAT
    AAGTTCCGTG TGCGTGCAAT CAATGTCTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCCT TGGGAGAGAA ACCTGAAGAA GAGCCGAAGG ACGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCTGA GGTCGACTAC CGGACCGTCA CGGTCAATAC TGAGCAGAAA
    GTGTCCGACT TCTACGACAT CGAAGAGAGA CTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TGGAAAAATC TGGGCAGGGA AATTCTTCAA GGCGTATTCG
    GCCAAGGAGA AAGAGAACAT CCGGCAGGAG ATCAGCATCA TGAACTGCCT GCACCACCCC
    AAGCTGGTCC AGTGTGTGGA CGCCTTCGAA GAGAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCCG GGGGTGAGCT GTTCGAGCGC ATCATTGATG AGGACTTTGA GCTGACGGAG
    CGGGAGTGCA TCAAGTACAT GAAGCAGATC TCAGAGGGGG TGGAGTACAT CCACAAGCAG
    GGCATCGTCC ACCTGGACCT CAAGCCCGAG AACATCATGT GTGTCAACAA GACAGGCACC
    AGGATCAAGC TCATCGACTT CGGTCTGGCC AGGAGGTTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCGGA GTTCGTCGCA CCCGAAGTGA TCAACTATGA GCCCATTGGC
    TATGCCACGG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTGGTCAG TGGACTGTCC
    CCCTTCATGG GTGACAATGA CAACGAAACC TTAGCCAACG TTACCTCAGC CACCTGGGAC
    TTTGACGACG AGGCCTTCGA CGAGATCTCC GATGACGCCA AGGATTTTAT CAGCAACTTG
    CTGAAGAAGG ATATGAAAAA CCGCCTGAAC TGCACCCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGACA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATCGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GATGTCTCCC AAGCTTTCCT GGAGGCTGTC
    GCTGAGGAGA AGCCCCATGT AAAACCCTAC TTTTCCAAGA CCATTCGTGA CTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCGGA CCCTGAAGTC
    GTCTGGTTCA AAGATGACCA ATCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGT GATGTCTGCG GGGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATCGTGGAA
    ACCATGGAGG AAGGAGAAGG AGAAGGGGGA GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218618
    Clone ID Related Accession (Same CDS sequence) XM_024994475.1 , NM_001372254.1
    Accession Version NM_001372254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Aug 27, 2019 this sequence version replaced XM_024994475.1. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.47381.1, SRR5571262.32085.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA TTTCAGGGAT CCCAAAACCT
    GAAGTGACCT GGTTCCTGGA CGGTGTCCCC GTGAGGAGTC AGGAGGGCAG CATCGAGGTT
    TACGAAGCTG GGGGGACCCA TTACCTCTGC CTGTGGAGAG CCCGGGCCCG GGACAGCGGG
    AGCTACAGCT GCACAGCCAC CAATGTCCGA GGCCAGGCGT CCTGTCGCTG GACGCTCCTG
    GTGAAAAAGC CAGCCGTGAT GGAGGTGGCC CCGTCATTCT CCAGTGCCCT GAAGGACTGC
    ACCGTCGTTG AGGGTCAGGA CTTTGTGCTG CGATGTTCAG TGCAGGGGAC CCCATTGCCT
    CGAATCACTT GGCTGCTCAA TGGACAGCCC ATCCAGTACG CCCACTCCAC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGACACC CTCCCAGAGG ATGATGGCAC CTACACCTGT
    CTGGCTGAGA ACACCGTGGG GCAGGTGTCC TGCAGCGCCC GCGTCACTGT CCAGGAGAAG
    AAGAGTGAGG AGAAGAGCGA GTACCTCCTG CCTGCAGCCC CCAACAAGCC CGTCGCGCCC
    CTCTTCCTGC AGGGCCTCTC TGATCTCCAA GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGAAACCC GCCCCCGGAG GTCATCTGGC TTCACGATGG GACTGAGATC
    CAGGAGTCCG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT GTGTATCCAG
    GAAGTGTTCC CAGAGGACAC GGGCACTTAT ACCTGTGAGG CCTGGAACAG CGCAGGAGAG
    GTCCGCACAC AGGCCGTGCT CACCGTGCAA GAGCCGCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCAGT GACTGCCTGC CTGGGCCAGA GTGTTCTTAT CTCCTGTGCC
    ATAGCCGGCG ACCCCTTCCC CACTGTGCAC TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG
    GACACTGGCC ACTTCGAGGT GCTACAGAAT GAGGACGTGT TCACCCTGGT TCTGAAAAAC
    GTGCAGCCCT GGCATGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGTGT TGGCCAATGC
    AGTTGCCAGG TGTCCCTGAT GCTACAGAGC AGCCCGGCCA GAGCGGTCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGTGGC CGAGGAGCTG GTGCTGATGG TGGTGGTGGT
    GGTGACGGCT ACGGGAGCCT GAGGCCTGGC TGGCCGGTGG CGAGAGGGCA AGGTGCCCCC
    GCAGAAGACG ACGGCGAGGA CGTGCGTGGG GTGCTCAAGA GGCGCGTGGA GACACGGCAG
    CACACGGAGG AGGCCGTTCG CCAGCAGGAG GTGGAGCAGC TGGACTTCCG CGACCTCCTG
    GGCAAGAAGG TGAGCACCAA GACCTTGTCT GAAGAGGACC TGAAGGAGAT CCCAGCCGAG
    CAGCTGGACT TCCGCGACCT CCTGGGCAAG AAGGTGAGTA CAAAGACCTT GTCTGAAGAG
    GACCTGAAGG AGATCCCAGC TGAGCAGATG GATTTCCGCG CCAACCTGCA GCGGCAGGTG
    AAGCCGAAGA CCCTGTCAGA GGAGGAGAGG AAAGTGCATG GCCCCCAGCA GGTGGACTTC
    CGCTCTGTCC TAGCCAAGAA GGGGACTCCC AAGACCCCTG TGCCTGAGAA GGTACCCCCT
    CCAAAACCGG CCACGCCAGA TTTTCGCTCA GTGTTGGGCA GTAAGAAAAA ATTACCAACA
    GAGAATGGTA GCAATAACAC TGAGGCCTTG AATGCCAAGG CAGCAGAAGG TCTCAAGCCT
    GTGGGCAATG CCCAGCCTTC AGGGTTCCTG AAACCCGTGG GCAACGCCAA GCTGGCTGAT
    ACCCCCAAAC CCTTGAGCAG CACCAAGCCA GCAGAGACCC CCAAACCCTT GGGTAATGTC
    AAGCCAGCTG AGACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAAAC CCCCAAACCC
    TTGGGCAGCA CCAAGCCAGC AGAGACCCCA AAACCCTTGG GTAACGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA CATCAAGCCA ACTGAGACCC CCAAACCCTT GGGCAGCACC
    AAGCCAGCAG AAACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAGAC CCCAAAACCC
    TTGGGTAATG TCAAGCCAGC CGAGACCCCC AAACCCTTGG GTAATGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA TGTCAAGCCA GCTGAGACCC CTAAACCTGT GAGCAATGCC
    AAGCCAGCCG AAACCCTCAA ACCTGTGGGC AACGCCAAGC CAGCTGAGAC CCCCAAACCC
    TTGAGTAACG TCAAGCCAGC TGAAACCCCC AAACTTGTGG GCAATGCCAA GCCAGCTGAG
    ACCTCTAAAC CCTTGGATAA CGCCAAGCCA GCTGAGGCCC CCAAACCCTT GGGCAATGCC
    AAGCCGGCTG AAATCCCAAA ACCCACTGGG AAAGAAGAAC TTAAGAAGGA GATTAAGAAT
    GATGTGAACT GCAAGAAGGG CCATGCAGGG GCCACAGATA GTGAAAAGAG ACCAGAGAGC
    CGAGGGACAG CCCCGACCTT CGAGGAGAAA CTACAAGATC TCCATGTGGC AGAGGGTCAG
    AAGCTGCTGC TCCAGTGCCG GGTGTCCTCT GACCCCCCAG CCACCATCAC TTGGACACTG
    AATGGAAAGA CGCTCAAGAC CACCAAGTTC ATCGTCCTCT CCCAAGAAGG CTCCTTGTGC
    TCCGTCTCCA TCGAGAAGGC ACTGCCCGAG GACAGAGGCT TATACAAGTG TGTAGCCAAG
    AACAGTGCCG GCCAGGCTGA GTCCTCCTGC CAAGTCACCG TGGACGTTCC AGATGCCCCC
    ACCAGTGAAA ATGCCAAGGC CCCGGAGATG AAAGCCCGGA GGCCGAAGAG CTCTCTTCCT
    CCAGTGCTAG GAACAGAAAG TGACGCAACT GTGAAAAAGA AACCTGCCCC CAAGACACCT
    CCGAAGGCAG CCATGCCGCC TCAGATCATC CAGTTTCCTG AGGACCAGAA GGTGCGAGCA
    GGAGAGTCTG TGGAGCTCTT TGGTAAGGTG GCCGGCACCC AGCCCATCAC CTGCACCTGG
    ATGAAGTTCC GAAAGCAGAT CCAGGACAGC GAGCACATCA AGGTGGAGAA CAGCGAGCAG
    GGCAGCAAGC TCACCATCCG GGCGGCACGC CAGGAGCACT GTGGCTGCTA CACCCTGCTG
    GTGGAGAACA AACTGGGCAG CAGGCAGGCC CAAGTCAACC TCACCGTGGT GGACAAGCCA
    GACCCCCCAG CCGGTACACC TTGTGCCTCT GACATCCGAA GCTCCTCACT GACCTTGTCC
    TGGTATGGCT CCTCGTATGA TGGGGGCAGC GCCGTACAGT CATACAGTGT CGAGATCTGG
    GACTCAGTGG ACAAGACGTG GAAGGAACTA GCCACGTGCC GCAGCACTTC TTTCAACGTC
    CAGGACCTGC TGCCTGACCG AGAATATAAG TTCCGTGTGC GTGCAATCAA TGTCTATGGA
    ACCAGTGAGC CAAGCCAGGA GTCTGAACTC ACAGCCTTGG GAGAGAAACC TGAAGAAGAG
    CCGAAGGACG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCTGAGGT CGACTACCGG
    ACCGTCACGG TCAATACTGA GCAGAAAGTG TCCGACTTCT ACGACATCGA AGAGAGACTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTGG AAAAATCTGG
    GCAGGGAAAT TCTTCAAGGC GTATTCGGCC AAGGAGAAAG AGAACATCCG GCAGGAGATC
    AGCATCATGA ACTGCCTGCA CCACCCCAAG CTGGTCCAGT GTGTGGACGC CTTCGAAGAG
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCCGGGG GTGAGCTGTT CGAGCGCATC
    ATTGATGAGG ACTTTGAGCT GACGGAGCGG GAGTGCATCA AGTACATGAA GCAGATCTCA
    GAGGGGGTGG AGTACATCCA CAAGCAGGGC ATCGTCCACC TGGACCTCAA GCCCGAGAAC
    ATCATGTGTG TCAACAAGAC AGGCACCAGG ATCAAGCTCA TCGACTTCGG TCTGGCCAGG
    AGGTTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCGGAGTT CGTCGCACCC
    GAAGTGATCA ACTATGAGCC CATTGGCTAT GCCACGGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TGGTCAGTGG ACTGTCCCCC TTCATGGGTG ACAATGACAA CGAAACCTTA
    GCCAACGTTA CCTCAGCCAC CTGGGACTTT GACGACGAGG CCTTCGACGA GATCTCCGAT
    GACGCCAAGG ATTTTATCAG CAACTTGCTG AAGAAGGATA TGAAAAACCG CCTGAACTGC
    ACCCAGTGCC TTCAGCATCC ATGGCTAATG AAAGACACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATCGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAA
    GATGTCTCCC AAGCTTTCCT GGAGGCTGTC GCTGAGGAGA AGCCCCATGT AAAACCCTAC
    TTTTCCAAGA CCATTCGTGA CTTAGAAGTT GTGGAGGGAA GTGCTGCTAG ATTTGACTGC
    AAGATTGAAG GATACCCGGA CCCTGAAGTC GTCTGGTTCA AAGATGACCA ATCAATCCGG
    GAGTCCCGCC ACTTCCAGAT AGACTACGAT GAGGATGGGA ACTGCTCTTT AATCATTAGT
    GATGTCTGCG GGGATGATGA TGCCAAGTAC ACCTGCAAGG CTGTCAACAG TCTTGGAGAA
    GCCACCTGCA CAGCAGAGCT CATCGTGGAA ACCATGGAGG AAGGAGAAGG AGAAGGGGGA
    GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359183.1
    CDS158..6118
    Translation

    Target ORF information:

    RefSeq Version NM_001372254.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA TTTCAGGGAT CCCAAAACCT
    GAAGTGACCT GGTTCCTGGA CGGTGTCCCC GTGAGGAGTC AGGAGGGCAG CATCGAGGTT
    TACGAAGCTG GGGGGACCCA TTACCTCTGC CTGTGGAGAG CCCGGGCCCG GGACAGCGGG
    AGCTACAGCT GCACAGCCAC CAATGTCCGA GGCCAGGCGT CCTGTCGCTG GACGCTCCTG
    GTGAAAAAGC CAGCCGTGAT GGAGGTGGCC CCGTCATTCT CCAGTGCCCT GAAGGACTGC
    ACCGTCGTTG AGGGTCAGGA CTTTGTGCTG CGATGTTCAG TGCAGGGGAC CCCATTGCCT
    CGAATCACTT GGCTGCTCAA TGGACAGCCC ATCCAGTACG CCCACTCCAC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGACACC CTCCCAGAGG ATGATGGCAC CTACACCTGT
    CTGGCTGAGA ACACCGTGGG GCAGGTGTCC TGCAGCGCCC GCGTCACTGT CCAGGAGAAG
    AAGAGTGAGG AGAAGAGCGA GTACCTCCTG CCTGCAGCCC CCAACAAGCC CGTCGCGCCC
    CTCTTCCTGC AGGGCCTCTC TGATCTCCAA GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGAAACCC GCCCCCGGAG GTCATCTGGC TTCACGATGG GACTGAGATC
    CAGGAGTCCG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT GTGTATCCAG
    GAAGTGTTCC CAGAGGACAC GGGCACTTAT ACCTGTGAGG CCTGGAACAG CGCAGGAGAG
    GTCCGCACAC AGGCCGTGCT CACCGTGCAA GAGCCGCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCAGT GACTGCCTGC CTGGGCCAGA GTGTTCTTAT CTCCTGTGCC
    ATAGCCGGCG ACCCCTTCCC CACTGTGCAC TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG
    GACACTGGCC ACTTCGAGGT GCTACAGAAT GAGGACGTGT TCACCCTGGT TCTGAAAAAC
    GTGCAGCCCT GGCATGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGTGT TGGCCAATGC
    AGTTGCCAGG TGTCCCTGAT GCTACAGAGC AGCCCGGCCA GAGCGGTCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGTGGC CGAGGAGCTG GTGCTGATGG TGGTGGTGGT
    GGTGACGGCT ACGGGAGCCT GAGGCCTGGC TGGCCGGTGG CGAGAGGGCA AGGTGCCCCC
    GCAGAAGACG ACGGCGAGGA CGTGCGTGGG GTGCTCAAGA GGCGCGTGGA GACACGGCAG
    CACACGGAGG AGGCCGTTCG CCAGCAGGAG GTGGAGCAGC TGGACTTCCG CGACCTCCTG
    GGCAAGAAGG TGAGCACCAA GACCTTGTCT GAAGAGGACC TGAAGGAGAT CCCAGCCGAG
    CAGCTGGACT TCCGCGACCT CCTGGGCAAG AAGGTGAGTA CAAAGACCTT GTCTGAAGAG
    GACCTGAAGG AGATCCCAGC TGAGCAGATG GATTTCCGCG CCAACCTGCA GCGGCAGGTG
    AAGCCGAAGA CCCTGTCAGA GGAGGAGAGG AAAGTGCATG GCCCCCAGCA GGTGGACTTC
    CGCTCTGTCC TAGCCAAGAA GGGGACTCCC AAGACCCCTG TGCCTGAGAA GGTACCCCCT
    CCAAAACCGG CCACGCCAGA TTTTCGCTCA GTGTTGGGCA GTAAGAAAAA ATTACCAACA
    GAGAATGGTA GCAATAACAC TGAGGCCTTG AATGCCAAGG CAGCAGAAGG TCTCAAGCCT
    GTGGGCAATG CCCAGCCTTC AGGGTTCCTG AAACCCGTGG GCAACGCCAA GCTGGCTGAT
    ACCCCCAAAC CCTTGAGCAG CACCAAGCCA GCAGAGACCC CCAAACCCTT GGGTAATGTC
    AAGCCAGCTG AGACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAAAC CCCCAAACCC
    TTGGGCAGCA CCAAGCCAGC AGAGACCCCA AAACCCTTGG GTAACGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA CATCAAGCCA ACTGAGACCC CCAAACCCTT GGGCAGCACC
    AAGCCAGCAG AAACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAGAC CCCAAAACCC
    TTGGGTAATG TCAAGCCAGC CGAGACCCCC AAACCCTTGG GTAATGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA TGTCAAGCCA GCTGAGACCC CTAAACCTGT GAGCAATGCC
    AAGCCAGCCG AAACCCTCAA ACCTGTGGGC AACGCCAAGC CAGCTGAGAC CCCCAAACCC
    TTGAGTAACG TCAAGCCAGC TGAAACCCCC AAACTTGTGG GCAATGCCAA GCCAGCTGAG
    ACCTCTAAAC CCTTGGATAA CGCCAAGCCA GCTGAGGCCC CCAAACCCTT GGGCAATGCC
    AAGCCGGCTG AAATCCCAAA ACCCACTGGG AAAGAAGAAC TTAAGAAGGA GATTAAGAAT
    GATGTGAACT GCAAGAAGGG CCATGCAGGG GCCACAGATA GTGAAAAGAG ACCAGAGAGC
    CGAGGGACAG CCCCGACCTT CGAGGAGAAA CTACAAGATC TCCATGTGGC AGAGGGTCAG
    AAGCTGCTGC TCCAGTGCCG GGTGTCCTCT GACCCCCCAG CCACCATCAC TTGGACACTG
    AATGGAAAGA CGCTCAAGAC CACCAAGTTC ATCGTCCTCT CCCAAGAAGG CTCCTTGTGC
    TCCGTCTCCA TCGAGAAGGC ACTGCCCGAG GACAGAGGCT TATACAAGTG TGTAGCCAAG
    AACAGTGCCG GCCAGGCTGA GTCCTCCTGC CAAGTCACCG TGGACGTTCC AGATGCCCCC
    ACCAGTGAAA ATGCCAAGGC CCCGGAGATG AAAGCCCGGA GGCCGAAGAG CTCTCTTCCT
    CCAGTGCTAG GAACAGAAAG TGACGCAACT GTGAAAAAGA AACCTGCCCC CAAGACACCT
    CCGAAGGCAG CCATGCCGCC TCAGATCATC CAGTTTCCTG AGGACCAGAA GGTGCGAGCA
    GGAGAGTCTG TGGAGCTCTT TGGTAAGGTG GCCGGCACCC AGCCCATCAC CTGCACCTGG
    ATGAAGTTCC GAAAGCAGAT CCAGGACAGC GAGCACATCA AGGTGGAGAA CAGCGAGCAG
    GGCAGCAAGC TCACCATCCG GGCGGCACGC CAGGAGCACT GTGGCTGCTA CACCCTGCTG
    GTGGAGAACA AACTGGGCAG CAGGCAGGCC CAAGTCAACC TCACCGTGGT GGACAAGCCA
    GACCCCCCAG CCGGTACACC TTGTGCCTCT GACATCCGAA GCTCCTCACT GACCTTGTCC
    TGGTATGGCT CCTCGTATGA TGGGGGCAGC GCCGTACAGT CATACAGTGT CGAGATCTGG
    GACTCAGTGG ACAAGACGTG GAAGGAACTA GCCACGTGCC GCAGCACTTC TTTCAACGTC
    CAGGACCTGC TGCCTGACCG AGAATATAAG TTCCGTGTGC GTGCAATCAA TGTCTATGGA
    ACCAGTGAGC CAAGCCAGGA GTCTGAACTC ACAGCCTTGG GAGAGAAACC TGAAGAAGAG
    CCGAAGGACG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCTGAGGT CGACTACCGG
    ACCGTCACGG TCAATACTGA GCAGAAAGTG TCCGACTTCT ACGACATCGA AGAGAGACTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTGG AAAAATCTGG
    GCAGGGAAAT TCTTCAAGGC GTATTCGGCC AAGGAGAAAG AGAACATCCG GCAGGAGATC
    AGCATCATGA ACTGCCTGCA CCACCCCAAG CTGGTCCAGT GTGTGGACGC CTTCGAAGAG
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCCGGGG GTGAGCTGTT CGAGCGCATC
    ATTGATGAGG ACTTTGAGCT GACGGAGCGG GAGTGCATCA AGTACATGAA GCAGATCTCA
    GAGGGGGTGG AGTACATCCA CAAGCAGGGC ATCGTCCACC TGGACCTCAA GCCCGAGAAC
    ATCATGTGTG TCAACAAGAC AGGCACCAGG ATCAAGCTCA TCGACTTCGG TCTGGCCAGG
    AGGTTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCGGAGTT CGTCGCACCC
    GAAGTGATCA ACTATGAGCC CATTGGCTAT GCCACGGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TGGTCAGTGG ACTGTCCCCC TTCATGGGTG ACAATGACAA CGAAACCTTA
    GCCAACGTTA CCTCAGCCAC CTGGGACTTT GACGACGAGG CCTTCGACGA GATCTCCGAT
    GACGCCAAGG ATTTTATCAG CAACTTGCTG AAGAAGGATA TGAAAAACCG CCTGAACTGC
    ACCCAGTGCC TTCAGCATCC ATGGCTAATG AAAGACACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATCGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAA
    GATGTCTCCC AAGCTTTCCT GGAGGCTGTC GCTGAGGAGA AGCCCCATGT AAAACCCTAC
    TTTTCCAAGA CCATTCGTGA CTTAGAAGTT GTGGAGGGAA GTGCTGCTAG ATTTGACTGC
    AAGATTGAAG GATACCCGGA CCCTGAAGTC GTCTGGTTCA AAGATGACCA ATCAATCCGG
    GAGTCCCGCC ACTTCCAGAT AGACTACGAT GAGGATGGGA ACTGCTCTTT AATCATTAGT
    GATGTCTGCG GGGATGATGA TGCCAAGTAC ACCTGCAAGG CTGTCAACAG TCTTGGAGAA
    GCCACCTGCA CAGCAGAGCT CATCGTGGAA ACCATGGAGG AAGGAGAAGG AGAAGGGGGA
    GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218618
    Clone ID Related Accession (Same CDS sequence) XM_024994475.1 , NM_001372254.1
    Accession Version XM_024994475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA TTTCAGGGAT CCCAAAACCT
    GAAGTGACCT GGTTCCTGGA CGGTGTCCCC GTGAGGAGTC AGGAGGGCAG CATCGAGGTT
    TACGAAGCTG GGGGGACCCA TTACCTCTGC CTGTGGAGAG CCCGGGCCCG GGACAGCGGG
    AGCTACAGCT GCACAGCCAC CAATGTCCGA GGCCAGGCGT CCTGTCGCTG GACGCTCCTG
    GTGAAAAAGC CAGCCGTGAT GGAGGTGGCC CCGTCATTCT CCAGTGCCCT GAAGGACTGC
    ACCGTCGTTG AGGGTCAGGA CTTTGTGCTG CGATGTTCAG TGCAGGGGAC CCCATTGCCT
    CGAATCACTT GGCTGCTCAA TGGACAGCCC ATCCAGTACG CCCACTCCAC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGACACC CTCCCAGAGG ATGATGGCAC CTACACCTGT
    CTGGCTGAGA ACACCGTGGG GCAGGTGTCC TGCAGCGCCC GCGTCACTGT CCAGGAGAAG
    AAGAGTGAGG AGAAGAGCGA GTACCTCCTG CCTGCAGCCC CCAACAAGCC CGTCGCGCCC
    CTCTTCCTGC AGGGCCTCTC TGATCTCCAA GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGAAACCC GCCCCCGGAG GTCATCTGGC TTCACGATGG GACTGAGATC
    CAGGAGTCCG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT GTGTATCCAG
    GAAGTGTTCC CAGAGGACAC GGGCACTTAT ACCTGTGAGG CCTGGAACAG CGCAGGAGAG
    GTCCGCACAC AGGCCGTGCT CACCGTGCAA GAGCCGCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCAGT GACTGCCTGC CTGGGCCAGA GTGTTCTTAT CTCCTGTGCC
    ATAGCCGGCG ACCCCTTCCC CACTGTGCAC TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG
    GACACTGGCC ACTTCGAGGT GCTACAGAAT GAGGACGTGT TCACCCTGGT TCTGAAAAAC
    GTGCAGCCCT GGCATGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGTGT TGGCCAATGC
    AGTTGCCAGG TGTCCCTGAT GCTACAGAGC AGCCCGGCCA GAGCGGTCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGTGGC CGAGGAGCTG GTGCTGATGG TGGTGGTGGT
    GGTGACGGCT ACGGGAGCCT GAGGCCTGGC TGGCCGGTGG CGAGAGGGCA AGGTGCCCCC
    GCAGAAGACG ACGGCGAGGA CGTGCGTGGG GTGCTCAAGA GGCGCGTGGA GACACGGCAG
    CACACGGAGG AGGCCGTTCG CCAGCAGGAG GTGGAGCAGC TGGACTTCCG CGACCTCCTG
    GGCAAGAAGG TGAGCACCAA GACCTTGTCT GAAGAGGACC TGAAGGAGAT CCCAGCCGAG
    CAGCTGGACT TCCGCGACCT CCTGGGCAAG AAGGTGAGTA CAAAGACCTT GTCTGAAGAG
    GACCTGAAGG AGATCCCAGC TGAGCAGATG GATTTCCGCG CCAACCTGCA GCGGCAGGTG
    AAGCCGAAGA CCCTGTCAGA GGAGGAGAGG AAAGTGCATG GCCCCCAGCA GGTGGACTTC
    CGCTCTGTCC TAGCCAAGAA GGGGACTCCC AAGACCCCTG TGCCTGAGAA GGTACCCCCT
    CCAAAACCGG CCACGCCAGA TTTTCGCTCA GTGTTGGGCA GTAAGAAAAA ATTACCAACA
    GAGAATGGTA GCAATAACAC TGAGGCCTTG AATGCCAAGG CAGCAGAAGG TCTCAAGCCT
    GTGGGCAATG CCCAGCCTTC AGGGTTCCTG AAACCCGTGG GCAACGCCAA GCTGGCTGAT
    ACCCCCAAAC CCTTGAGCAG CACCAAGCCA GCAGAGACCC CCAAACCCTT GGGTAATGTC
    AAGCCAGCTG AGACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAAAC CCCCAAACCC
    TTGGGCAGCA CCAAGCCAGC AGAGACCCCA AAACCCTTGG GTAACGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA CATCAAGCCA ACTGAGACCC CCAAACCCTT GGGCAGCACC
    AAGCCAGCAG AAACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAGAC CCCAAAACCC
    TTGGGTAATG TCAAGCCAGC CGAGACCCCC AAACCCTTGG GTAATGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA TGTCAAGCCA GCTGAGACCC CTAAACCTGT GAGCAATGCC
    AAGCCAGCCG AAACCCTCAA ACCTGTGGGC AACGCCAAGC CAGCTGAGAC CCCCAAACCC
    TTGAGTAACG TCAAGCCAGC TGAAACCCCC AAACTTGTGG GCAATGCCAA GCCAGCTGAG
    ACCTCTAAAC CCTTGGATAA CGCCAAGCCA GCTGAGGCCC CCAAACCCTT GGGCAATGCC
    AAGCCGGCTG AAATCCCAAA ACCCACTGGG AAAGAAGAAC TTAAGAAGGA GATTAAGAAT
    GATGTGAACT GCAAGAAGGG CCATGCAGGG GCCACAGATA GTGAAAAGAG ACCAGAGAGC
    CGAGGGACAG CCCCGACCTT CGAGGAGAAA CTACAAGATC TCCATGTGGC AGAGGGTCAG
    AAGCTGCTGC TCCAGTGCCG GGTGTCCTCT GACCCCCCAG CCACCATCAC TTGGACACTG
    AATGGAAAGA CGCTCAAGAC CACCAAGTTC ATCGTCCTCT CCCAAGAAGG CTCCTTGTGC
    TCCGTCTCCA TCGAGAAGGC ACTGCCCGAG GACAGAGGCT TATACAAGTG TGTAGCCAAG
    AACAGTGCCG GCCAGGCTGA GTCCTCCTGC CAAGTCACCG TGGACGTTCC AGATGCCCCC
    ACCAGTGAAA ATGCCAAGGC CCCGGAGATG AAAGCCCGGA GGCCGAAGAG CTCTCTTCCT
    CCAGTGCTAG GAACAGAAAG TGACGCAACT GTGAAAAAGA AACCTGCCCC CAAGACACCT
    CCGAAGGCAG CCATGCCGCC TCAGATCATC CAGTTTCCTG AGGACCAGAA GGTGCGAGCA
    GGAGAGTCTG TGGAGCTCTT TGGTAAGGTG GCCGGCACCC AGCCCATCAC CTGCACCTGG
    ATGAAGTTCC GAAAGCAGAT CCAGGACAGC GAGCACATCA AGGTGGAGAA CAGCGAGCAG
    GGCAGCAAGC TCACCATCCG GGCGGCACGC CAGGAGCACT GTGGCTGCTA CACCCTGCTG
    GTGGAGAACA AACTGGGCAG CAGGCAGGCC CAAGTCAACC TCACCGTGGT GGACAAGCCA
    GACCCCCCAG CCGGTACACC TTGTGCCTCT GACATCCGAA GCTCCTCACT GACCTTGTCC
    TGGTATGGCT CCTCGTATGA TGGGGGCAGC GCCGTACAGT CATACAGTGT CGAGATCTGG
    GACTCAGTGG ACAAGACGTG GAAGGAACTA GCCACGTGCC GCAGCACTTC TTTCAACGTC
    CAGGACCTGC TGCCTGACCG AGAATATAAG TTCCGTGTGC GTGCAATCAA TGTCTATGGA
    ACCAGTGAGC CAAGCCAGGA GTCTGAACTC ACAGCCTTGG GAGAGAAACC TGAAGAAGAG
    CCGAAGGACG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCTGAGGT CGACTACCGG
    ACCGTCACGG TCAATACTGA GCAGAAAGTG TCCGACTTCT ACGACATCGA AGAGAGACTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTGG AAAAATCTGG
    GCAGGGAAAT TCTTCAAGGC GTATTCGGCC AAGGAGAAAG AGAACATCCG GCAGGAGATC
    AGCATCATGA ACTGCCTGCA CCACCCCAAG CTGGTCCAGT GTGTGGACGC CTTCGAAGAG
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCCGGGG GTGAGCTGTT CGAGCGCATC
    ATTGATGAGG ACTTTGAGCT GACGGAGCGG GAGTGCATCA AGTACATGAA GCAGATCTCA
    GAGGGGGTGG AGTACATCCA CAAGCAGGGC ATCGTCCACC TGGACCTCAA GCCCGAGAAC
    ATCATGTGTG TCAACAAGAC AGGCACCAGG ATCAAGCTCA TCGACTTCGG TCTGGCCAGG
    AGGTTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCGGAGTT CGTCGCACCC
    GAAGTGATCA ACTATGAGCC CATTGGCTAT GCCACGGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TGGTCAGTGG ACTGTCCCCC TTCATGGGTG ACAATGACAA CGAAACCTTA
    GCCAACGTTA CCTCAGCCAC CTGGGACTTT GACGACGAGG CCTTCGACGA GATCTCCGAT
    GACGCCAAGG ATTTTATCAG CAACTTGCTG AAGAAGGATA TGAAAAACCG CCTGAACTGC
    ACCCAGTGCC TTCAGCATCC ATGGCTAATG AAAGACACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATCGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAA
    GATGTCTCCC AAGCTTTCCT GGAGGCTGTC GCTGAGGAGA AGCCCCATGT AAAACCCTAC
    TTTTCCAAGA CCATTCGTGA CTTAGAAGTT GTGGAGGGAA GTGCTGCTAG ATTTGACTGC
    AAGATTGAAG GATACCCGGA CCCTGAAGTC GTCTGGTTCA AAGATGACCA ATCAATCCGG
    GAGTCCCGCC ACTTCCAGAT AGACTACGAT GAGGATGGGA ACTGCTCTTT AATCATTAGT
    GATGTCTGCG GGGATGATGA TGCCAAGTAC ACCTGCAAGG CTGTCAACAG TCTTGGAGAA
    GCCACCTGCA CAGCAGAGCT CATCGTGGAA ACCATGGAGG AAGGAGAAGG AGAAGGGGGA
    GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850243.1
    CDS152..6112
    Translation

    Target ORF information:

    RefSeq Version XM_024994475.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA TTTCAGGGAT CCCAAAACCT
    GAAGTGACCT GGTTCCTGGA CGGTGTCCCC GTGAGGAGTC AGGAGGGCAG CATCGAGGTT
    TACGAAGCTG GGGGGACCCA TTACCTCTGC CTGTGGAGAG CCCGGGCCCG GGACAGCGGG
    AGCTACAGCT GCACAGCCAC CAATGTCCGA GGCCAGGCGT CCTGTCGCTG GACGCTCCTG
    GTGAAAAAGC CAGCCGTGAT GGAGGTGGCC CCGTCATTCT CCAGTGCCCT GAAGGACTGC
    ACCGTCGTTG AGGGTCAGGA CTTTGTGCTG CGATGTTCAG TGCAGGGGAC CCCATTGCCT
    CGAATCACTT GGCTGCTCAA TGGACAGCCC ATCCAGTACG CCCACTCCAC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGACACC CTCCCAGAGG ATGATGGCAC CTACACCTGT
    CTGGCTGAGA ACACCGTGGG GCAGGTGTCC TGCAGCGCCC GCGTCACTGT CCAGGAGAAG
    AAGAGTGAGG AGAAGAGCGA GTACCTCCTG CCTGCAGCCC CCAACAAGCC CGTCGCGCCC
    CTCTTCCTGC AGGGCCTCTC TGATCTCCAA GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGAAACCC GCCCCCGGAG GTCATCTGGC TTCACGATGG GACTGAGATC
    CAGGAGTCCG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT GTGTATCCAG
    GAAGTGTTCC CAGAGGACAC GGGCACTTAT ACCTGTGAGG CCTGGAACAG CGCAGGAGAG
    GTCCGCACAC AGGCCGTGCT CACCGTGCAA GAGCCGCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCAGT GACTGCCTGC CTGGGCCAGA GTGTTCTTAT CTCCTGTGCC
    ATAGCCGGCG ACCCCTTCCC CACTGTGCAC TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG
    GACACTGGCC ACTTCGAGGT GCTACAGAAT GAGGACGTGT TCACCCTGGT TCTGAAAAAC
    GTGCAGCCCT GGCATGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGTGT TGGCCAATGC
    AGTTGCCAGG TGTCCCTGAT GCTACAGAGC AGCCCGGCCA GAGCGGTCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGTGGC CGAGGAGCTG GTGCTGATGG TGGTGGTGGT
    GGTGACGGCT ACGGGAGCCT GAGGCCTGGC TGGCCGGTGG CGAGAGGGCA AGGTGCCCCC
    GCAGAAGACG ACGGCGAGGA CGTGCGTGGG GTGCTCAAGA GGCGCGTGGA GACACGGCAG
    CACACGGAGG AGGCCGTTCG CCAGCAGGAG GTGGAGCAGC TGGACTTCCG CGACCTCCTG
    GGCAAGAAGG TGAGCACCAA GACCTTGTCT GAAGAGGACC TGAAGGAGAT CCCAGCCGAG
    CAGCTGGACT TCCGCGACCT CCTGGGCAAG AAGGTGAGTA CAAAGACCTT GTCTGAAGAG
    GACCTGAAGG AGATCCCAGC TGAGCAGATG GATTTCCGCG CCAACCTGCA GCGGCAGGTG
    AAGCCGAAGA CCCTGTCAGA GGAGGAGAGG AAAGTGCATG GCCCCCAGCA GGTGGACTTC
    CGCTCTGTCC TAGCCAAGAA GGGGACTCCC AAGACCCCTG TGCCTGAGAA GGTACCCCCT
    CCAAAACCGG CCACGCCAGA TTTTCGCTCA GTGTTGGGCA GTAAGAAAAA ATTACCAACA
    GAGAATGGTA GCAATAACAC TGAGGCCTTG AATGCCAAGG CAGCAGAAGG TCTCAAGCCT
    GTGGGCAATG CCCAGCCTTC AGGGTTCCTG AAACCCGTGG GCAACGCCAA GCTGGCTGAT
    ACCCCCAAAC CCTTGAGCAG CACCAAGCCA GCAGAGACCC CCAAACCCTT GGGTAATGTC
    AAGCCAGCTG AGACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAAAC CCCCAAACCC
    TTGGGCAGCA CCAAGCCAGC AGAGACCCCA AAACCCTTGG GTAACGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA CATCAAGCCA ACTGAGACCC CCAAACCCTT GGGCAGCACC
    AAGCCAGCAG AAACCCCCAA ACCCTTGGGC AGCACCAAGC CAGCAGAGAC CCCAAAACCC
    TTGGGTAATG TCAAGCCAGC CGAGACCCCC AAACCCTTGG GTAATGTCAA GCCAGCTGAG
    ACCCCCAAAC CCTTGGGTAA TGTCAAGCCA GCTGAGACCC CTAAACCTGT GAGCAATGCC
    AAGCCAGCCG AAACCCTCAA ACCTGTGGGC AACGCCAAGC CAGCTGAGAC CCCCAAACCC
    TTGAGTAACG TCAAGCCAGC TGAAACCCCC AAACTTGTGG GCAATGCCAA GCCAGCTGAG
    ACCTCTAAAC CCTTGGATAA CGCCAAGCCA GCTGAGGCCC CCAAACCCTT GGGCAATGCC
    AAGCCGGCTG AAATCCCAAA ACCCACTGGG AAAGAAGAAC TTAAGAAGGA GATTAAGAAT
    GATGTGAACT GCAAGAAGGG CCATGCAGGG GCCACAGATA GTGAAAAGAG ACCAGAGAGC
    CGAGGGACAG CCCCGACCTT CGAGGAGAAA CTACAAGATC TCCATGTGGC AGAGGGTCAG
    AAGCTGCTGC TCCAGTGCCG GGTGTCCTCT GACCCCCCAG CCACCATCAC TTGGACACTG
    AATGGAAAGA CGCTCAAGAC CACCAAGTTC ATCGTCCTCT CCCAAGAAGG CTCCTTGTGC
    TCCGTCTCCA TCGAGAAGGC ACTGCCCGAG GACAGAGGCT TATACAAGTG TGTAGCCAAG
    AACAGTGCCG GCCAGGCTGA GTCCTCCTGC CAAGTCACCG TGGACGTTCC AGATGCCCCC
    ACCAGTGAAA ATGCCAAGGC CCCGGAGATG AAAGCCCGGA GGCCGAAGAG CTCTCTTCCT
    CCAGTGCTAG GAACAGAAAG TGACGCAACT GTGAAAAAGA AACCTGCCCC CAAGACACCT
    CCGAAGGCAG CCATGCCGCC TCAGATCATC CAGTTTCCTG AGGACCAGAA GGTGCGAGCA
    GGAGAGTCTG TGGAGCTCTT TGGTAAGGTG GCCGGCACCC AGCCCATCAC CTGCACCTGG
    ATGAAGTTCC GAAAGCAGAT CCAGGACAGC GAGCACATCA AGGTGGAGAA CAGCGAGCAG
    GGCAGCAAGC TCACCATCCG GGCGGCACGC CAGGAGCACT GTGGCTGCTA CACCCTGCTG
    GTGGAGAACA AACTGGGCAG CAGGCAGGCC CAAGTCAACC TCACCGTGGT GGACAAGCCA
    GACCCCCCAG CCGGTACACC TTGTGCCTCT GACATCCGAA GCTCCTCACT GACCTTGTCC
    TGGTATGGCT CCTCGTATGA TGGGGGCAGC GCCGTACAGT CATACAGTGT CGAGATCTGG
    GACTCAGTGG ACAAGACGTG GAAGGAACTA GCCACGTGCC GCAGCACTTC TTTCAACGTC
    CAGGACCTGC TGCCTGACCG AGAATATAAG TTCCGTGTGC GTGCAATCAA TGTCTATGGA
    ACCAGTGAGC CAAGCCAGGA GTCTGAACTC ACAGCCTTGG GAGAGAAACC TGAAGAAGAG
    CCGAAGGACG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCTGAGGT CGACTACCGG
    ACCGTCACGG TCAATACTGA GCAGAAAGTG TCCGACTTCT ACGACATCGA AGAGAGACTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTGG AAAAATCTGG
    GCAGGGAAAT TCTTCAAGGC GTATTCGGCC AAGGAGAAAG AGAACATCCG GCAGGAGATC
    AGCATCATGA ACTGCCTGCA CCACCCCAAG CTGGTCCAGT GTGTGGACGC CTTCGAAGAG
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCCGGGG GTGAGCTGTT CGAGCGCATC
    ATTGATGAGG ACTTTGAGCT GACGGAGCGG GAGTGCATCA AGTACATGAA GCAGATCTCA
    GAGGGGGTGG AGTACATCCA CAAGCAGGGC ATCGTCCACC TGGACCTCAA GCCCGAGAAC
    ATCATGTGTG TCAACAAGAC AGGCACCAGG ATCAAGCTCA TCGACTTCGG TCTGGCCAGG
    AGGTTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCGGAGTT CGTCGCACCC
    GAAGTGATCA ACTATGAGCC CATTGGCTAT GCCACGGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TGGTCAGTGG ACTGTCCCCC TTCATGGGTG ACAATGACAA CGAAACCTTA
    GCCAACGTTA CCTCAGCCAC CTGGGACTTT GACGACGAGG CCTTCGACGA GATCTCCGAT
    GACGCCAAGG ATTTTATCAG CAACTTGCTG AAGAAGGATA TGAAAAACCG CCTGAACTGC
    ACCCAGTGCC TTCAGCATCC ATGGCTAATG AAAGACACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATCGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAA
    GATGTCTCCC AAGCTTTCCT GGAGGCTGTC GCTGAGGAGA AGCCCCATGT AAAACCCTAC
    TTTTCCAAGA CCATTCGTGA CTTAGAAGTT GTGGAGGGAA GTGCTGCTAG ATTTGACTGC
    AAGATTGAAG GATACCCGGA CCCTGAAGTC GTCTGGTTCA AAGATGACCA ATCAATCCGG
    GAGTCCCGCC ACTTCCAGAT AGACTACGAT GAGGATGGGA ACTGCTCTTT AATCATTAGT
    GATGTCTGCG GGGATGATGA TGCCAAGTAC ACCTGCAAGG CTGTCAACAG TCTTGGAGAA
    GCCACCTGCA CAGCAGAGCT CATCGTGGAA ACCATGGAGG AAGGAGAAGG AGAAGGGGGA
    GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218615
    Clone ID Related Accession (Same CDS sequence) XM_024994340.1
    Accession Version XM_024994340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA GTAAGAACCG CCCCAGCACC
    CAGCGAGGCG GCTCCCCGAC CTGGGCCACA GGGAGCCAGC CTCAACCCCC CAGGGAATCT
    GAGCTGGAAC CACTGGGGAA CTCCCCCAGA AAGGGCCAGA GGGCCCCCGT CCTGCAAAAG
    ACCTCCAGCA CCATCACCCT GCAGGCAGCA AAAGTCCAGC TGGAGCCCAG AGCGCCAGTC
    CCAGGCACGC CCTGTCCTTC CAGAGAAGAG ATGGAGGGGC CAGCTGCGTC CCCTCCAGAC
    ACCCTCTCCA CCAGGCGGTC AGGCCTGGGA AGCCAGGACA CCATGGGCAA GGTTGCTACC
    AGGAAGGTCC CCATGGAGAG CCAGAGGGAC ACCATGTTTC CCAAATTTGA GAACAAGCCC
    CAGAGCCAGG AGGTCAGTGA AGATGAGCCA GTCGAGTTCA GATGCGAGGT TTCAGGGATC
    CCAAAACCTG AAGTGACCTG GTTCCTGGAC GGTGTCCCCG TGAGGAGTCA GGAGGGCAGC
    ATCGAGGTTT ACGAAGCTGG GGGGACCCAT TACCTCTGCC TGTGGAGAGC CCGGGCCCGG
    GACAGCGGGA GCTACAGCTG CACAGCCACC AATGTCCGAG GCCAGGCGTC CTGTCGCTGG
    ACGCTCCTGG TGAAAAAGCC AGCCGTGATG GAGGTGGCCC CGTCATTCTC CAGTGCCCTG
    AAGGACTGCA CCGTCGTTGA GGGTCAGGAC TTTGTGCTGC GATGTTCAGT GCAGGGGACC
    CCATTGCCTC GAATCACTTG GCTGCTCAAT GGACAGCCCA TCCAGTACGC CCACTCCACC
    TGCGAGGCTG GCGTGGCCGA GCTCCACATC CAGGACACCC TCCCAGAGGA TGATGGCACC
    TACACCTGTC TGGCTGAGAA CACCGTGGGG CAGGTGTCCT GCAGCGCCCG CGTCACTGTC
    CAGGAGAAGA AGAGTGAGGA GAAGAGCGAG TACCTCCTGC CTGCAGCCCC CAACAAGCCC
    GTCGCGCCCC TCTTCCTGCA GGGCCTCTCT GATCTCCAAG TCATGGATGG GAGCCAGGTC
    ACCATGACAG TCCAGGTGTC AGGAAACCCG CCCCCGGAGG TCATCTGGCT TCACGATGGG
    ACTGAGATCC AGGAGTCCGA GGACTTCCAC TTTGAACAGA GAGGCACTCA GCACAGCCTG
    TGTATCCAGG AAGTGTTCCC AGAGGACACG GGCACTTATA CCTGTGAGGC CTGGAACAGC
    GCAGGAGAGG TCCGCACACA GGCCGTGCTC ACCGTGCAAG AGCCGCAGGA TGGCACCCAG
    CCCTGGTTCA TCAGCAAGCC CCGCTCAGTG ACTGCCTGCC TGGGCCAGAG TGTTCTTATC
    TCCTGTGCCA TAGCCGGCGA CCCCTTCCCC ACTGTGCACT GGCTCCGAGA TGGCAAAGCC
    CTCTCCAGGG ACACTGGCCA CTTCGAGGTG CTACAGAATG AGGACGTGTT CACCCTGGTT
    CTGAAAAACG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGTGTT
    GGCCAATGCA GTTGCCAGGT GTCCCTGATG CTACAGAGCA GCCCGGCCAG AGCGGTCCTG
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC CTCTGTGGCC GAGGAGCTGG TGCTGATGGT
    GGTGGTGGTG GTGACGGCTA CGGGAGCCTG AGGCCTGGCT GGCCGGTGGC GAGAGGGCAA
    GGTGCCCCCG CAGAAGACGA CGGCGAGGAC GTGCGTGGGG TGCTCAAGAG GCGCGTGGAG
    ACACGGCAGC ACACGGAGGA GGCCGTTCGC CAGCAGGAGG TGGAGCAGCT GGACTTCCGC
    GACCTCCTGG GCAAGAAGGT GAGCACCAAG ACCTTGTCTG AAGAGGACCT GAAGGAGATC
    CCAGCCGAGC AGCTGGACTT CCGCGACCTC CTGGGCAAGA AGGTGAGTAC AAAGACCTTG
    TCTGAAGAGG ACCTGAAGGA GATCCCAGCT GAGCAGATGG ATTTCCGCGC CAACCTGCAG
    CGGCAGGTGA AGCCGAAGAC CCTGTCAGAG GAGGAGAGGA AAGTGCATGG CCCCCAGCAG
    GTGGACTTCC GCTCTGTCCT AGCCAAGAAG GGGACTCCCA AGACCCCTGT GCCTGAGAAG
    GTACCCCCTC CAAAACCGGC CACGCCAGAT TTTCGCTCAG TGTTGGGCAG TAAGAAAAAA
    TTACCAACAG AGAATGGTAG CAATAACACT GAGGCCTTGA ATGCCAAGGC AGCAGAAGGT
    CTCAAGCCTG TGGGCAATGC CCAGCCTTCA GGGTTCCTGA AACCCGTGGG CAACGCCAAG
    CTGGCTGATA CCCCCAAACC CTTGAGCAGC ACCAAGCCAG CAGAGACCCC CAAACCCTTG
    GGTAATGTCA AGCCAGCTGA GACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAAACC
    CCCAAACCCT TGGGCAGCAC CAAGCCAGCA GAGACCCCAA AACCCTTGGG TAACGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAC ATCAAGCCAA CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAATGT CAAGCCAGCC GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC TAAACCTGTG
    AGCAATGCCA AGCCAGCCGA AACCCTCAAA CCTGTGGGCA ACGCCAAGCC AGCTGAGACC
    CCCAAACCCT TGAGTAACGT CAAGCCAGCT GAAACCCCCA AACTTGTGGG CAATGCCAAG
    CCAGCTGAGA CCTCTAAACC CTTGGATAAC GCCAAGCCAG CTGAGGCCCC CAAACCCTTG
    GGCAATGCCA AGCCGGCTGA AATCCCAAAA CCCACTGGGA AAGAAGAACT TAAGAAGGAG
    ATTAAGAATG ATGTGAACTG CAAGAAGGGC CATGCAGGGG CCACAGATAG TGAAAAGAGA
    CCAGAGAGCC GAGGGACAGC CCCGACCTTC GAGGAGAAAC TACAAGATCT CCATGTGGCA
    GAGGGTCAGA AGCTGCTGCT CCAGTGCCGG GTGTCCTCTG ACCCCCCAGC CACCATCACT
    TGGACACTGA ATGGAAAGAC GCTCAAGACC ACCAAGTTCA TCGTCCTCTC CCAAGAAGGC
    TCCTTGTGCT CCGTCTCCAT CGAGAAGGCA CTGCCCGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ACAGTGCCGG CCAGGCTGAG TCCTCCTGCC AAGTCACCGT GGACGTTCCA
    GATGCCCCCA CCAGTGAAAA TGCCAAGGCC CCGGAGATGA AAGCCCGGAG GCCGAAGAGC
    TCTCTTCCTC CAGTGCTAGG AACAGAAAGT GACGCAACTG TGAAAAAGAA ACCTGCCCCC
    AAGACACCTC CGAAGGCAGC CATGCCGCCT CAGATCATCC AGTTTCCTGA GGACCAGAAG
    GTGCGAGCAG GAGAGTCTGT GGAGCTCTTT GGTAAGGTGG CCGGCACCCA GCCCATCACC
    TGCACCTGGA TGAAGTTCCG AAAGCAGATC CAGGACAGCG AGCACATCAA GGTGGAGAAC
    AGCGAGCAGG GCAGCAAGCT CACCATCCGG GCGGCACGCC AGGAGCACTG TGGCTGCTAC
    ACCCTGCTGG TGGAGAACAA ACTGGGCAGC AGGCAGGCCC AAGTCAACCT CACCGTGGTG
    GACAAGCCAG ACCCCCCAGC CGGTACACCT TGTGCCTCTG ACATCCGAAG CTCCTCACTG
    ACCTTGTCCT GGTATGGCTC CTCGTATGAT GGGGGCAGCG CCGTACAGTC ATACAGTGTC
    GAGATCTGGG ACTCAGTGGA CAAGACGTGG AAGGAACTAG CCACGTGCCG CAGCACTTCT
    TTCAACGTCC AGGACCTGCT GCCTGACCGA GAATATAAGT TCCGTGTGCG TGCAATCAAT
    GTCTATGGAA CCAGTGAGCC AAGCCAGGAG TCTGAACTCA CAGCCTTGGG AGAGAAACCT
    GAAGAAGAGC CGAAGGACGA AGTGGAGGTG TCAGATGATG ATGAGAAGGA GCCTGAGGTC
    GACTACCGGA CCGTCACGGT CAATACTGAG CAGAAAGTGT CCGACTTCTA CGACATCGAA
    GAGAGACTAG GATCTGGGAA ATTTGGACAG GTCTTTCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCG TATTCGGCCA AGGAGAAAGA GAACATCCGG
    CAGGAGATCA GCATCATGAA CTGCCTGCAC CACCCCAAGC TGGTCCAGTG TGTGGACGCC
    TTCGAAGAGA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCCGGGGG TGAGCTGTTC
    GAGCGCATCA TTGATGAGGA CTTTGAGCTG ACGGAGCGGG AGTGCATCAA GTACATGAAG
    CAGATCTCAG AGGGGGTGGA GTACATCCAC AAGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGTGT CAACAAGACA GGCACCAGGA TCAAGCTCAT CGACTTCGGT
    CTGGCCAGGA GGTTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGCAC CCCGGAGTTC
    GTCGCACCCG AAGTGATCAA CTATGAGCCC ATTGGCTATG CCACGGACAT GTGGAGCATC
    GGGGTCATCT GCTACATCCT GGTCAGTGGA CTGTCCCCCT TCATGGGTGA CAATGACAAC
    GAAACCTTAG CCAACGTTAC CTCAGCCACC TGGGACTTTG ACGACGAGGC CTTCGACGAG
    ATCTCCGATG ACGCCAAGGA TTTTATCAGC AACTTGCTGA AGAAGGATAT GAAAAACCGC
    CTGAACTGCA CCCAGTGCCT TCAGCATCCA TGGCTAATGA AAGACACCAA GAACATGGAG
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGGTCACCA ACCAGCCCGC TCAATGCAGA AAAACTAGAA
    TCTGAAGAAG ATGTCTCCCA AGCTTTCCTG GAGGCTGTCG CTGAGGAGAA GCCCCATGTA
    AAACCCTACT TTTCCAAGAC CATTCGTGAC TTAGAAGTTG TGGAGGGAAG TGCTGCTAGA
    TTTGACTGCA AGATTGAAGG ATACCCGGAC CCTGAAGTCG TCTGGTTCAA AGATGACCAA
    TCAATCCGGG AGTCCCGCCA CTTCCAGATA GACTACGATG AGGATGGGAA CTGCTCTTTA
    ATCATTAGTG ATGTCTGCGG GGATGATGAT GCCAAGTACA CCTGCAAGGC TGTCAACAGT
    CTTGGAGAAG CCACCTGCAC AGCAGAGCTC ATCGTGGAAA CCATGGAGGA AGGAGAAGGA
    GAAGGGGGAG AAGAGGAGGA GGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850108.1
    CDS165..6554
    Translation

    Target ORF information:

    RefSeq Version XM_024994340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA GTAAGAACCG CCCCAGCACC
    CAGCGAGGCG GCTCCCCGAC CTGGGCCACA GGGAGCCAGC CTCAACCCCC CAGGGAATCT
    GAGCTGGAAC CACTGGGGAA CTCCCCCAGA AAGGGCCAGA GGGCCCCCGT CCTGCAAAAG
    ACCTCCAGCA CCATCACCCT GCAGGCAGCA AAAGTCCAGC TGGAGCCCAG AGCGCCAGTC
    CCAGGCACGC CCTGTCCTTC CAGAGAAGAG ATGGAGGGGC CAGCTGCGTC CCCTCCAGAC
    ACCCTCTCCA CCAGGCGGTC AGGCCTGGGA AGCCAGGACA CCATGGGCAA GGTTGCTACC
    AGGAAGGTCC CCATGGAGAG CCAGAGGGAC ACCATGTTTC CCAAATTTGA GAACAAGCCC
    CAGAGCCAGG AGGTCAGTGA AGATGAGCCA GTCGAGTTCA GATGCGAGGT TTCAGGGATC
    CCAAAACCTG AAGTGACCTG GTTCCTGGAC GGTGTCCCCG TGAGGAGTCA GGAGGGCAGC
    ATCGAGGTTT ACGAAGCTGG GGGGACCCAT TACCTCTGCC TGTGGAGAGC CCGGGCCCGG
    GACAGCGGGA GCTACAGCTG CACAGCCACC AATGTCCGAG GCCAGGCGTC CTGTCGCTGG
    ACGCTCCTGG TGAAAAAGCC AGCCGTGATG GAGGTGGCCC CGTCATTCTC CAGTGCCCTG
    AAGGACTGCA CCGTCGTTGA GGGTCAGGAC TTTGTGCTGC GATGTTCAGT GCAGGGGACC
    CCATTGCCTC GAATCACTTG GCTGCTCAAT GGACAGCCCA TCCAGTACGC CCACTCCACC
    TGCGAGGCTG GCGTGGCCGA GCTCCACATC CAGGACACCC TCCCAGAGGA TGATGGCACC
    TACACCTGTC TGGCTGAGAA CACCGTGGGG CAGGTGTCCT GCAGCGCCCG CGTCACTGTC
    CAGGAGAAGA AGAGTGAGGA GAAGAGCGAG TACCTCCTGC CTGCAGCCCC CAACAAGCCC
    GTCGCGCCCC TCTTCCTGCA GGGCCTCTCT GATCTCCAAG TCATGGATGG GAGCCAGGTC
    ACCATGACAG TCCAGGTGTC AGGAAACCCG CCCCCGGAGG TCATCTGGCT TCACGATGGG
    ACTGAGATCC AGGAGTCCGA GGACTTCCAC TTTGAACAGA GAGGCACTCA GCACAGCCTG
    TGTATCCAGG AAGTGTTCCC AGAGGACACG GGCACTTATA CCTGTGAGGC CTGGAACAGC
    GCAGGAGAGG TCCGCACACA GGCCGTGCTC ACCGTGCAAG AGCCGCAGGA TGGCACCCAG
    CCCTGGTTCA TCAGCAAGCC CCGCTCAGTG ACTGCCTGCC TGGGCCAGAG TGTTCTTATC
    TCCTGTGCCA TAGCCGGCGA CCCCTTCCCC ACTGTGCACT GGCTCCGAGA TGGCAAAGCC
    CTCTCCAGGG ACACTGGCCA CTTCGAGGTG CTACAGAATG AGGACGTGTT CACCCTGGTT
    CTGAAAAACG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGTGTT
    GGCCAATGCA GTTGCCAGGT GTCCCTGATG CTACAGAGCA GCCCGGCCAG AGCGGTCCTG
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC CTCTGTGGCC GAGGAGCTGG TGCTGATGGT
    GGTGGTGGTG GTGACGGCTA CGGGAGCCTG AGGCCTGGCT GGCCGGTGGC GAGAGGGCAA
    GGTGCCCCCG CAGAAGACGA CGGCGAGGAC GTGCGTGGGG TGCTCAAGAG GCGCGTGGAG
    ACACGGCAGC ACACGGAGGA GGCCGTTCGC CAGCAGGAGG TGGAGCAGCT GGACTTCCGC
    GACCTCCTGG GCAAGAAGGT GAGCACCAAG ACCTTGTCTG AAGAGGACCT GAAGGAGATC
    CCAGCCGAGC AGCTGGACTT CCGCGACCTC CTGGGCAAGA AGGTGAGTAC AAAGACCTTG
    TCTGAAGAGG ACCTGAAGGA GATCCCAGCT GAGCAGATGG ATTTCCGCGC CAACCTGCAG
    CGGCAGGTGA AGCCGAAGAC CCTGTCAGAG GAGGAGAGGA AAGTGCATGG CCCCCAGCAG
    GTGGACTTCC GCTCTGTCCT AGCCAAGAAG GGGACTCCCA AGACCCCTGT GCCTGAGAAG
    GTACCCCCTC CAAAACCGGC CACGCCAGAT TTTCGCTCAG TGTTGGGCAG TAAGAAAAAA
    TTACCAACAG AGAATGGTAG CAATAACACT GAGGCCTTGA ATGCCAAGGC AGCAGAAGGT
    CTCAAGCCTG TGGGCAATGC CCAGCCTTCA GGGTTCCTGA AACCCGTGGG CAACGCCAAG
    CTGGCTGATA CCCCCAAACC CTTGAGCAGC ACCAAGCCAG CAGAGACCCC CAAACCCTTG
    GGTAATGTCA AGCCAGCTGA GACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAAACC
    CCCAAACCCT TGGGCAGCAC CAAGCCAGCA GAGACCCCAA AACCCTTGGG TAACGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAC ATCAAGCCAA CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAATGT CAAGCCAGCC GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC TAAACCTGTG
    AGCAATGCCA AGCCAGCCGA AACCCTCAAA CCTGTGGGCA ACGCCAAGCC AGCTGAGACC
    CCCAAACCCT TGAGTAACGT CAAGCCAGCT GAAACCCCCA AACTTGTGGG CAATGCCAAG
    CCAGCTGAGA CCTCTAAACC CTTGGATAAC GCCAAGCCAG CTGAGGCCCC CAAACCCTTG
    GGCAATGCCA AGCCGGCTGA AATCCCAAAA CCCACTGGGA AAGAAGAACT TAAGAAGGAG
    ATTAAGAATG ATGTGAACTG CAAGAAGGGC CATGCAGGGG CCACAGATAG TGAAAAGAGA
    CCAGAGAGCC GAGGGACAGC CCCGACCTTC GAGGAGAAAC TACAAGATCT CCATGTGGCA
    GAGGGTCAGA AGCTGCTGCT CCAGTGCCGG GTGTCCTCTG ACCCCCCAGC CACCATCACT
    TGGACACTGA ATGGAAAGAC GCTCAAGACC ACCAAGTTCA TCGTCCTCTC CCAAGAAGGC
    TCCTTGTGCT CCGTCTCCAT CGAGAAGGCA CTGCCCGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ACAGTGCCGG CCAGGCTGAG TCCTCCTGCC AAGTCACCGT GGACGTTCCA
    GATGCCCCCA CCAGTGAAAA TGCCAAGGCC CCGGAGATGA AAGCCCGGAG GCCGAAGAGC
    TCTCTTCCTC CAGTGCTAGG AACAGAAAGT GACGCAACTG TGAAAAAGAA ACCTGCCCCC
    AAGACACCTC CGAAGGCAGC CATGCCGCCT CAGATCATCC AGTTTCCTGA GGACCAGAAG
    GTGCGAGCAG GAGAGTCTGT GGAGCTCTTT GGTAAGGTGG CCGGCACCCA GCCCATCACC
    TGCACCTGGA TGAAGTTCCG AAAGCAGATC CAGGACAGCG AGCACATCAA GGTGGAGAAC
    AGCGAGCAGG GCAGCAAGCT CACCATCCGG GCGGCACGCC AGGAGCACTG TGGCTGCTAC
    ACCCTGCTGG TGGAGAACAA ACTGGGCAGC AGGCAGGCCC AAGTCAACCT CACCGTGGTG
    GACAAGCCAG ACCCCCCAGC CGGTACACCT TGTGCCTCTG ACATCCGAAG CTCCTCACTG
    ACCTTGTCCT GGTATGGCTC CTCGTATGAT GGGGGCAGCG CCGTACAGTC ATACAGTGTC
    GAGATCTGGG ACTCAGTGGA CAAGACGTGG AAGGAACTAG CCACGTGCCG CAGCACTTCT
    TTCAACGTCC AGGACCTGCT GCCTGACCGA GAATATAAGT TCCGTGTGCG TGCAATCAAT
    GTCTATGGAA CCAGTGAGCC AAGCCAGGAG TCTGAACTCA CAGCCTTGGG AGAGAAACCT
    GAAGAAGAGC CGAAGGACGA AGTGGAGGTG TCAGATGATG ATGAGAAGGA GCCTGAGGTC
    GACTACCGGA CCGTCACGGT CAATACTGAG CAGAAAGTGT CCGACTTCTA CGACATCGAA
    GAGAGACTAG GATCTGGGAA ATTTGGACAG GTCTTTCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCG TATTCGGCCA AGGAGAAAGA GAACATCCGG
    CAGGAGATCA GCATCATGAA CTGCCTGCAC CACCCCAAGC TGGTCCAGTG TGTGGACGCC
    TTCGAAGAGA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCCGGGGG TGAGCTGTTC
    GAGCGCATCA TTGATGAGGA CTTTGAGCTG ACGGAGCGGG AGTGCATCAA GTACATGAAG
    CAGATCTCAG AGGGGGTGGA GTACATCCAC AAGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGTGT CAACAAGACA GGCACCAGGA TCAAGCTCAT CGACTTCGGT
    CTGGCCAGGA GGTTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGCAC CCCGGAGTTC
    GTCGCACCCG AAGTGATCAA CTATGAGCCC ATTGGCTATG CCACGGACAT GTGGAGCATC
    GGGGTCATCT GCTACATCCT GGTCAGTGGA CTGTCCCCCT TCATGGGTGA CAATGACAAC
    GAAACCTTAG CCAACGTTAC CTCAGCCACC TGGGACTTTG ACGACGAGGC CTTCGACGAG
    ATCTCCGATG ACGCCAAGGA TTTTATCAGC AACTTGCTGA AGAAGGATAT GAAAAACCGC
    CTGAACTGCA CCCAGTGCCT TCAGCATCCA TGGCTAATGA AAGACACCAA GAACATGGAG
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGGTCACCA ACCAGCCCGC TCAATGCAGA AAAACTAGAA
    TCTGAAGAAG ATGTCTCCCA AGCTTTCCTG GAGGCTGTCG CTGAGGAGAA GCCCCATGTA
    AAACCCTACT TTTCCAAGAC CATTCGTGAC TTAGAAGTTG TGGAGGGAAG TGCTGCTAGA
    TTTGACTGCA AGATTGAAGG ATACCCGGAC CCTGAAGTCG TCTGGTTCAA AGATGACCAA
    TCAATCCGGG AGTCCCGCCA CTTCCAGATA GACTACGATG AGGATGGGAA CTGCTCTTTA
    ATCATTAGTG ATGTCTGCGG GGATGATGAT GCCAAGTACA CCTGCAAGGC TGTCAACAGT
    CTTGGAGAAG CCACCTGCAC AGCAGAGCTC ATCGTGGAAA CCATGGAGGA AGGAGAAGGA
    GAAGGGGGAG AAGAGGAGGA GGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218616
    Clone ID Related Accession (Same CDS sequence) XM_024994382.1
    Accession Version XM_024994382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA GTAAGAACCG CCCCAGCACC
    CAGCGAGGCG GCTCCCCGAC CTGGGCCACA GGGAGCCAGC CTCAACCCCC CAGGGAATCT
    GAGCTGGAAC CACTGGGGAA CTCCCCCAGA AAGGGCCAGA GGGCCCCCGT CCTGCAAAAG
    ACCTCCAGCA CCATCACCCT GCAGGCAGCA AAAGTCCAGC TGGAGCCCAG AGCGCCAGTC
    CCAGGCACGC CCTGTCCTTC CAGAGAAGAG ATGGAGGGGC CAGCTGCGTC CCCTCCAGAC
    ACCCTCTCCA CCAGGCGGTC AGGCCTGGGA AGCCAGGACA CCATGGGCAA GGTTGCTACC
    AGGAAGGTCC CCATGGAGAG CCAGAGGGAC ACCATGTTTC CCAAATTTGA GAACAAGCCC
    CAGAGCCAGG AGGTCAGTGA AGATGAGCCA GTCGAGTTCA GATGCGAGGT TTCAGGGATC
    CCAAAACCTG AAGTGACCTG GTTCCTGGAC GGTGTCCCCG TGAGGAGTCA GGAGGGCAGC
    ATCGAGGTTT ACGAAGCTGG GGGGACCCAT TACCTCTGCC TGTGGAGAGC CCGGGCCCGG
    GACAGCGGGA GCTACAGCTG CACAGCCACC AATGTCCGAG GCCAGGCGTC CTGTCGCTGG
    ACGCTCCTGG TGAAAAAGCC AGCCGTGATG GAGGTGGCCC CGTCATTCTC CAGTGCCCTG
    AAGGACTGCA CCGTCGTTGA GGGTCAGGAC TTTGTGCTGC GATGTTCAGT GCAGGGGACC
    CCATTGCCTC GAATCACTTG GCTGCTCAAT GGACAGCCCA TCCAGTACGC CCACTCCACC
    TGCGAGGCTG GCGTGGCCGA GCTCCACATC CAGGACACCC TCCCAGAGGA TGATGGCACC
    TACACCTGTC TGGCTGAGAA CACCGTGGGG CAGGTGTCCT GCAGCGCCCG CGTCACTGTC
    CAGGAGAAGA AGAGTGAGGA GAAGAGCGAG TACCTCCTGC CTGCAGCCCC CAACAAGCCC
    GTCGCGCCCC TCTTCCTGCA GGGCCTCTCT GATCTCCAAG TCATGGATGG GAGCCAGGTC
    ACCATGACAG TCCAGGTGTC AGGAAACCCG CCCCCGGAGG TCATCTGGCT TCACGATGGG
    ACTGAGATCC AGGAGTCCGA GGACTTCCAC TTTGAACAGA GAGGCACTCA GCACAGCCTG
    TGTATCCAGG AAGTGTTCCC AGAGGACACG GGCACTTATA CCTGTGAGGC CTGGAACAGC
    GCAGGAGAGG TCCGCACACA GGCCGTGCTC ACCGTGCAAG AGCCGCAGGA TGGCACCCAG
    CCCTGGTTCA TCAGCAAGCC CCGCTCAGTG ACTGCCTGCC TGGGCCAGAG TGTTCTTATC
    TCCTGTGCCA TAGCCGGCGA CCCCTTCCCC ACTGTGCACT GGCTCCGAGA TGGCAAAGCC
    CTCTCCAGGG ACACTGGCCA CTTCGAGGTG CTACAGAATG AGGACGTGTT CACCCTGGTT
    CTGAAAAACG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGTGTT
    GGCCAATGCA GTTGCCAGGT GTCCCTGATG CTACAGAGCA GCCCGGCCAG AGCGGTCCTG
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC CTCTGTGGCC GAGGAGCTGG TGCTGATGGT
    GGTGGTGGTG GTGACGGCTA CGGGAGCCTG AGGCCTGGCT GGCCGGTGGC GAGAGGGCAA
    GGTGCCCCCG CAGAAGACGA CGGCGAGGAC GTGCGTGGGG TGCTCAAGAG GCGCGTGGAG
    ACACGGCAGC ACACGGAGGA GGCCGTTCGC CAGCAGGAGG TGGAGCAGCT GGACTTCCGC
    GACCTCCTGG GCAAGAAGGT GAGCACCAAG ACCTTGTCTG AAGAGGACCT GAAGGAGATC
    CCAGCCGAGC AGCTGGACTT CCGCGACCTC CTGGGCAAGA AGGTGAGTAC AAAGACCTTG
    TCTGAAGAGG ACCTGAAGGA GATCCCAGCT GAGCAGATGG ATTTCCGCGC CAACCTGCAG
    CGGCAGGTGA AGCCGAAGAC CCTGTCAGAG GAGGAGAGGA AAGTGCATGG CCCCCAGCAG
    GTGGACTTCC GCTCTGTCCT AGCCAAGAAG GGGACTCCCA AGACCCCTGT GCCTGAGAAG
    GTACCCCCTC CAAAACCGGC CACGCCAGAT TTTCGCTCAG TGTTGGGCAG TAAGAAAAAA
    TTACCAACAG AGAATGGTAG CAATAACACT GAGGCCTTGA ATGCCAAGGC AGCAGAAGGT
    CTCAAGCCTG TGGGCAATGC CCAGCCTTCA GGGTTCCTGA AACCCGTGGG CAACGCCAAG
    CTGGCTGATA CCCCCAAACC CTTGAGCAGC ACCAAGCCAG CAGAGACCCC CAAACCCTTG
    GGTAATGTCA AGCCAGCTGA GACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAAACC
    CCCAAACCCT TGGGCAGCAC CAAGCCAGCA GAGACCCCAA AACCCTTGGG TAACGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAC ATCAAGCCAA CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAATGT CAAGCCAGCC GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC TAAACCTGTG
    AGCAATGCCA AGCCAGCCGA AACCCTCAAA CCTGTGGGCA ACGCCAAGCC AGCTGAGACC
    CCCAAACCCT TGAGTAACGT CAAGCCAGCT GAAACCCCCA AACTTGTGGG CAATGCCAAG
    CCAGCTGAGA CCTCTAAACC CTTGGATAAC GCCAAGCCAG CTGAGGCCCC CAAACCCTTG
    GGCAATGCCA AGCCGGCTGA AATCCCAAAA CCCACTGGGA AAGAAGAACT TAAGAAGGAG
    ATTAAGAATG ATGTGAACTG CAAGAAGGGC CATGCAGGGG CCACAGATAG TGAAAAGAGA
    CCAGAGAGCC GAGGGACAGC CCCGACCTTC GAGGAGAAAC TACAAGATCT CCATGTGGCA
    GAGGGTCAGA AGCTGCTGCT CCAGTGCCGG GTGTCCTCTG ACCCCCCAGC CACCATCACT
    TGGACACTGA ATGGAAAGAC GCTCAAGACC ACCAAGTTCA TCGTCCTCTC CCAAGAAGGC
    TCCTTGTGCT CCGTCTCCAT CGAGAAGGCA CTGCCCGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ACAGTGCCGG CCAGGCTGAG TCCTCCTGCC AAGTCACCGT GGACGTTCCA
    GATGCCCCCA CCAGTGAAAA TGCCAAGGCC CCGGAGATGA AAGCCCGGAG GCCGAAGAGC
    TCTCTTCCTC CAGTGCTAGG AACAGAAAGT GACGCAACTG TGAAAAAGAA ACCTGCCCCC
    AAGACACCTC CGAAGGCAGC CATGCCGCCT CAGATCATCC AGTTTCCTGA GGACCAGAAG
    GTGCGAGCAG GAGAGTCTGT GGAGCTCTTT GGTAAGGTGG CCGGCACCCA GCCCATCACC
    TGCACCTGGA TGAAGTTCCG AAAGCAGATC CAGGACAGCG AGCACATCAA GGTGGAGAAC
    AGCGAGCAGG GCAGCAAGCT CACCATCCGG GCGGCACGCC AGGAGCACTG TGGCTGCTAC
    ACCCTGCTGG TGGAGAACAA ACTGGGCAGC AGGCAGGCCC AAGTCAACCT CACCGTGGTG
    GACAAGCCAG ACCCCCCAGC CGGTACACCT TGTGCCTCTG ACATCCGAAG CTCCTCACTG
    ACCTTGTCCT GGTATGGCTC CTCGTATGAT GGGGGCAGCG CCGTACAGTC ATACAGTGTC
    GAGATCTGGG ACTCAGTGGA CAAGACGTGG AAGGAACTAG CCACGTGCCG CAGCACTTCT
    TTCAACGTCC AGGACCTGCT GCCTGACCGA GAATATAAGT TCCGTGTGCG TGCAATCAAT
    GTCTATGGAA CCAGTGAGCC AAGCCAGGAG TCTGAACTCA CAGCCTTGGG AGAGAAACCT
    GAAGAAGAGC CGAAGGACGA AGTGGAGGTG TCAGATGATG ATGAGAAGGA GCCTGAGGTC
    GACTACCGGA CCGTCACGGT CAATACTGAG CAGAAAGTGT CCGACTTCTA CGACATCGAA
    GAGAGACTAG GATCTGGGAA ATTTGGACAG GTCTTTCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCG TATTCGGCCA AGGAGAAAGA GAACATCCGG
    CAGGAGATCA GCATCATGAA CTGCCTGCAC CACCCCAAGC TGGTCCAGTG TGTGGACGCC
    TTCGAAGAGA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCCGGGGG TGAGCTGTTC
    GAGCGCATCA TTGATGAGGA CTTTGAGCTG ACGGAGCGGG AGTGCATCAA GTACATGAAG
    CAGATCTCAG AGGGGGTGGA GTACATCCAC AAGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGTGT CAACAAGACA GGCACCAGGA TCAAGCTCAT CGACTTCGGT
    CTGGCCAGGA GGTTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGCAC CCCGGAGTTC
    GTCGCACCCG AAGTGATCAA CTATGAGCCC ATTGGCTATG CCACGGACAT GTGGAGCATC
    GGGGTCATCT GCTACATCCT GGTCAGTGGA CTGTCCCCCT TCATGGGTGA CAATGACAAC
    GAAACCTTAG CCAACGTTAC CTCAGCCACC TGGGACTTTG ACGACGAGGC CTTCGACGAG
    ATCTCCGATG ACGCCAAGGA TTTTATCAGC AACTTGCTGA AGAAGGATAT GAAAAACCGC
    CTGAACTGCA CCCAGTGCCT TCAGCATCCA TGGCTAATGA AAGACACCAA GAACATGGAG
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGGTCACCA ACCAGCCCGC TCAATGCAGA AAAACTAGAA
    TCTGAAGATG TCTCCCAAGC TTTCCTGGAG GCTGTCGCTG AGGAGAAGCC CCATGTAAAA
    CCCTACTTTT CCAAGACCAT TCGTGACTTA GAAGTTGTGG AGGGAAGTGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCGGACCCT GAAGTCGTCT GGTTCAAAGA TGACCAATCA
    ATCCGGGAGT CCCGCCACTT CCAGATAGAC TACGATGAGG ATGGGAACTG CTCTTTAATC
    ATTAGTGATG TCTGCGGGGA TGATGATGCC AAGTACACCT GCAAGGCTGT CAACAGTCTT
    GGAGAAGCCA CCTGCACAGC AGAGCTCATC GTGGAAACCA TGGAGGAAGG AGAAGGAGAA
    GGGGGAGAAG AGGAGGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850150.1
    CDS96..6482
    Translation

    Target ORF information:

    RefSeq Version XM_024994382.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA GTAAGAACCG CCCCAGCACC
    CAGCGAGGCG GCTCCCCGAC CTGGGCCACA GGGAGCCAGC CTCAACCCCC CAGGGAATCT
    GAGCTGGAAC CACTGGGGAA CTCCCCCAGA AAGGGCCAGA GGGCCCCCGT CCTGCAAAAG
    ACCTCCAGCA CCATCACCCT GCAGGCAGCA AAAGTCCAGC TGGAGCCCAG AGCGCCAGTC
    CCAGGCACGC CCTGTCCTTC CAGAGAAGAG ATGGAGGGGC CAGCTGCGTC CCCTCCAGAC
    ACCCTCTCCA CCAGGCGGTC AGGCCTGGGA AGCCAGGACA CCATGGGCAA GGTTGCTACC
    AGGAAGGTCC CCATGGAGAG CCAGAGGGAC ACCATGTTTC CCAAATTTGA GAACAAGCCC
    CAGAGCCAGG AGGTCAGTGA AGATGAGCCA GTCGAGTTCA GATGCGAGGT TTCAGGGATC
    CCAAAACCTG AAGTGACCTG GTTCCTGGAC GGTGTCCCCG TGAGGAGTCA GGAGGGCAGC
    ATCGAGGTTT ACGAAGCTGG GGGGACCCAT TACCTCTGCC TGTGGAGAGC CCGGGCCCGG
    GACAGCGGGA GCTACAGCTG CACAGCCACC AATGTCCGAG GCCAGGCGTC CTGTCGCTGG
    ACGCTCCTGG TGAAAAAGCC AGCCGTGATG GAGGTGGCCC CGTCATTCTC CAGTGCCCTG
    AAGGACTGCA CCGTCGTTGA GGGTCAGGAC TTTGTGCTGC GATGTTCAGT GCAGGGGACC
    CCATTGCCTC GAATCACTTG GCTGCTCAAT GGACAGCCCA TCCAGTACGC CCACTCCACC
    TGCGAGGCTG GCGTGGCCGA GCTCCACATC CAGGACACCC TCCCAGAGGA TGATGGCACC
    TACACCTGTC TGGCTGAGAA CACCGTGGGG CAGGTGTCCT GCAGCGCCCG CGTCACTGTC
    CAGGAGAAGA AGAGTGAGGA GAAGAGCGAG TACCTCCTGC CTGCAGCCCC CAACAAGCCC
    GTCGCGCCCC TCTTCCTGCA GGGCCTCTCT GATCTCCAAG TCATGGATGG GAGCCAGGTC
    ACCATGACAG TCCAGGTGTC AGGAAACCCG CCCCCGGAGG TCATCTGGCT TCACGATGGG
    ACTGAGATCC AGGAGTCCGA GGACTTCCAC TTTGAACAGA GAGGCACTCA GCACAGCCTG
    TGTATCCAGG AAGTGTTCCC AGAGGACACG GGCACTTATA CCTGTGAGGC CTGGAACAGC
    GCAGGAGAGG TCCGCACACA GGCCGTGCTC ACCGTGCAAG AGCCGCAGGA TGGCACCCAG
    CCCTGGTTCA TCAGCAAGCC CCGCTCAGTG ACTGCCTGCC TGGGCCAGAG TGTTCTTATC
    TCCTGTGCCA TAGCCGGCGA CCCCTTCCCC ACTGTGCACT GGCTCCGAGA TGGCAAAGCC
    CTCTCCAGGG ACACTGGCCA CTTCGAGGTG CTACAGAATG AGGACGTGTT CACCCTGGTT
    CTGAAAAACG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGTGTT
    GGCCAATGCA GTTGCCAGGT GTCCCTGATG CTACAGAGCA GCCCGGCCAG AGCGGTCCTG
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC CTCTGTGGCC GAGGAGCTGG TGCTGATGGT
    GGTGGTGGTG GTGACGGCTA CGGGAGCCTG AGGCCTGGCT GGCCGGTGGC GAGAGGGCAA
    GGTGCCCCCG CAGAAGACGA CGGCGAGGAC GTGCGTGGGG TGCTCAAGAG GCGCGTGGAG
    ACACGGCAGC ACACGGAGGA GGCCGTTCGC CAGCAGGAGG TGGAGCAGCT GGACTTCCGC
    GACCTCCTGG GCAAGAAGGT GAGCACCAAG ACCTTGTCTG AAGAGGACCT GAAGGAGATC
    CCAGCCGAGC AGCTGGACTT CCGCGACCTC CTGGGCAAGA AGGTGAGTAC AAAGACCTTG
    TCTGAAGAGG ACCTGAAGGA GATCCCAGCT GAGCAGATGG ATTTCCGCGC CAACCTGCAG
    CGGCAGGTGA AGCCGAAGAC CCTGTCAGAG GAGGAGAGGA AAGTGCATGG CCCCCAGCAG
    GTGGACTTCC GCTCTGTCCT AGCCAAGAAG GGGACTCCCA AGACCCCTGT GCCTGAGAAG
    GTACCCCCTC CAAAACCGGC CACGCCAGAT TTTCGCTCAG TGTTGGGCAG TAAGAAAAAA
    TTACCAACAG AGAATGGTAG CAATAACACT GAGGCCTTGA ATGCCAAGGC AGCAGAAGGT
    CTCAAGCCTG TGGGCAATGC CCAGCCTTCA GGGTTCCTGA AACCCGTGGG CAACGCCAAG
    CTGGCTGATA CCCCCAAACC CTTGAGCAGC ACCAAGCCAG CAGAGACCCC CAAACCCTTG
    GGTAATGTCA AGCCAGCTGA GACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAAACC
    CCCAAACCCT TGGGCAGCAC CAAGCCAGCA GAGACCCCAA AACCCTTGGG TAACGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAC ATCAAGCCAA CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAATGT CAAGCCAGCC GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC TAAACCTGTG
    AGCAATGCCA AGCCAGCCGA AACCCTCAAA CCTGTGGGCA ACGCCAAGCC AGCTGAGACC
    CCCAAACCCT TGAGTAACGT CAAGCCAGCT GAAACCCCCA AACTTGTGGG CAATGCCAAG
    CCAGCTGAGA CCTCTAAACC CTTGGATAAC GCCAAGCCAG CTGAGGCCCC CAAACCCTTG
    GGCAATGCCA AGCCGGCTGA AATCCCAAAA CCCACTGGGA AAGAAGAACT TAAGAAGGAG
    ATTAAGAATG ATGTGAACTG CAAGAAGGGC CATGCAGGGG CCACAGATAG TGAAAAGAGA
    CCAGAGAGCC GAGGGACAGC CCCGACCTTC GAGGAGAAAC TACAAGATCT CCATGTGGCA
    GAGGGTCAGA AGCTGCTGCT CCAGTGCCGG GTGTCCTCTG ACCCCCCAGC CACCATCACT
    TGGACACTGA ATGGAAAGAC GCTCAAGACC ACCAAGTTCA TCGTCCTCTC CCAAGAAGGC
    TCCTTGTGCT CCGTCTCCAT CGAGAAGGCA CTGCCCGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ACAGTGCCGG CCAGGCTGAG TCCTCCTGCC AAGTCACCGT GGACGTTCCA
    GATGCCCCCA CCAGTGAAAA TGCCAAGGCC CCGGAGATGA AAGCCCGGAG GCCGAAGAGC
    TCTCTTCCTC CAGTGCTAGG AACAGAAAGT GACGCAACTG TGAAAAAGAA ACCTGCCCCC
    AAGACACCTC CGAAGGCAGC CATGCCGCCT CAGATCATCC AGTTTCCTGA GGACCAGAAG
    GTGCGAGCAG GAGAGTCTGT GGAGCTCTTT GGTAAGGTGG CCGGCACCCA GCCCATCACC
    TGCACCTGGA TGAAGTTCCG AAAGCAGATC CAGGACAGCG AGCACATCAA GGTGGAGAAC
    AGCGAGCAGG GCAGCAAGCT CACCATCCGG GCGGCACGCC AGGAGCACTG TGGCTGCTAC
    ACCCTGCTGG TGGAGAACAA ACTGGGCAGC AGGCAGGCCC AAGTCAACCT CACCGTGGTG
    GACAAGCCAG ACCCCCCAGC CGGTACACCT TGTGCCTCTG ACATCCGAAG CTCCTCACTG
    ACCTTGTCCT GGTATGGCTC CTCGTATGAT GGGGGCAGCG CCGTACAGTC ATACAGTGTC
    GAGATCTGGG ACTCAGTGGA CAAGACGTGG AAGGAACTAG CCACGTGCCG CAGCACTTCT
    TTCAACGTCC AGGACCTGCT GCCTGACCGA GAATATAAGT TCCGTGTGCG TGCAATCAAT
    GTCTATGGAA CCAGTGAGCC AAGCCAGGAG TCTGAACTCA CAGCCTTGGG AGAGAAACCT
    GAAGAAGAGC CGAAGGACGA AGTGGAGGTG TCAGATGATG ATGAGAAGGA GCCTGAGGTC
    GACTACCGGA CCGTCACGGT CAATACTGAG CAGAAAGTGT CCGACTTCTA CGACATCGAA
    GAGAGACTAG GATCTGGGAA ATTTGGACAG GTCTTTCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCG TATTCGGCCA AGGAGAAAGA GAACATCCGG
    CAGGAGATCA GCATCATGAA CTGCCTGCAC CACCCCAAGC TGGTCCAGTG TGTGGACGCC
    TTCGAAGAGA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCCGGGGG TGAGCTGTTC
    GAGCGCATCA TTGATGAGGA CTTTGAGCTG ACGGAGCGGG AGTGCATCAA GTACATGAAG
    CAGATCTCAG AGGGGGTGGA GTACATCCAC AAGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGTGT CAACAAGACA GGCACCAGGA TCAAGCTCAT CGACTTCGGT
    CTGGCCAGGA GGTTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGCAC CCCGGAGTTC
    GTCGCACCCG AAGTGATCAA CTATGAGCCC ATTGGCTATG CCACGGACAT GTGGAGCATC
    GGGGTCATCT GCTACATCCT GGTCAGTGGA CTGTCCCCCT TCATGGGTGA CAATGACAAC
    GAAACCTTAG CCAACGTTAC CTCAGCCACC TGGGACTTTG ACGACGAGGC CTTCGACGAG
    ATCTCCGATG ACGCCAAGGA TTTTATCAGC AACTTGCTGA AGAAGGATAT GAAAAACCGC
    CTGAACTGCA CCCAGTGCCT TCAGCATCCA TGGCTAATGA AAGACACCAA GAACATGGAG
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGGTCACCA ACCAGCCCGC TCAATGCAGA AAAACTAGAA
    TCTGAAGATG TCTCCCAAGC TTTCCTGGAG GCTGTCGCTG AGGAGAAGCC CCATGTAAAA
    CCCTACTTTT CCAAGACCAT TCGTGACTTA GAAGTTGTGG AGGGAAGTGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCGGACCCT GAAGTCGTCT GGTTCAAAGA TGACCAATCA
    ATCCGGGAGT CCCGCCACTT CCAGATAGAC TACGATGAGG ATGGGAACTG CTCTTTAATC
    ATTAGTGATG TCTGCGGGGA TGATGATGCC AAGTACACCT GCAAGGCTGT CAACAGTCTT
    GGAGAAGCCA CCTGCACAGC AGAGCTCATC GTGGAAACCA TGGAGGAAGG AGAAGGAGAA
    GGGGGAGAAG AGGAGGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218617
    Clone ID Related Accession (Same CDS sequence) XM_024994421.1
    Accession Version XM_024994421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA GTAAGAACCG CCCCAGCACC
    CAGCGAGGCG GCTCCCCGAC CTGGGCCACA GGGAGCCAGC CTCAACCCCC CAGGGAATCT
    GAGCTGGAAC CACTGGGGAA CTCCCCCAGA AAGGGCCAGA GGGCCCCCGT CCTGCAAAAG
    ACCTCCAGCA CCATCACCCT GCAGGCAGCA AAAGTCCAGC TGGAGCCCAG AGCGCCAGTC
    CCAGGCACGC CCTGTCCTTC CAGAGAAGAG ATGGAGGGGC CAGCTGCGTC CCCTCCAGAC
    ACCCTCTCCA CCAGGCGGTC AGGCCTGGGA AGCCAGGACA CCATGGGCAA GGTTGCTACC
    AGGAAGGTCC CCATGGAGAG CCAGAGGGAC ACCATGTTTC CCAAATTTGA GAACAAGCCC
    CAGAGCCAGG AGGTCAGTGA AGATGAGCCA GTCGAGTTCA GATGCGAGGT TTCAGGGATC
    CCAAAACCTG AAGTGACCTG GTTCCTGGAC GGTGTCCCCG TGAGGAGTCA GGAGGGCAGC
    ATCGAGGTTT ACGAAGCTGG GGGGACCCAT TACCTCTGCC TGTGGAGAGC CCGGGCCCGG
    GACAGCGGGA GCTACAGCTG CACAGCCACC AATGTCCGAG GCCAGGCGTC CTGTCGCTGG
    ACGCTCCTGG TGAAAAAGCC AGCCGTGATG GAGGTGGCCC CGTCATTCTC CAGTGCCCTG
    AAGGACTGCA CCGTCGTTGA GGGTCAGGAC TTTGTGCTGC GATGTTCAGT GCAGGGGACC
    CCATTGCCTC GAATCACTTG GCTGCTCAAT GGACAGCCCA TCCAGTACGC CCACTCCACC
    TGCGAGGCTG GCGTGGCCGA GCTCCACATC CAGGACACCC TCCCAGAGGA TGATGGCACC
    TACACCTGTC TGGCTGAGAA CACCGTGGGG CAGGTGTCCT GCAGCGCCCG CGTCACTGTC
    CAGGAGAAGA AGAGTGAGGA GAAGAGCGAG TACCTCCTGC CTGCAGCCCC CAACAAGCCC
    GTCGCGCCCC TCTTCCTGCA GGGCCTCTCT GATCTCCAAG TCATGGATGG GAGCCAGGTC
    ACCATGACAG TCCAGGTGTC AGGAAACCCG CCCCCGGAGG TCATCTGGCT TCACGATGGG
    ACTGAGATCC AGGAGTCCGA GGACTTCCAC TTTGAACAGA GAGGCACTCA GCACAGCCTG
    TGTATCCAGG AAGTGTTCCC AGAGGACACG GGCACTTATA CCTGTGAGGC CTGGAACAGC
    GCAGGAGAGG TCCGCACACA GGCCGTGCTC ACCGTGCAAG AGCCGCAGGA TGGCACCCAG
    CCCTGGTTCA TCAGCAAGCC CCGCTCAGTG ACTGCCTGCC TGGGCCAGAG TGTTCTTATC
    TCCTGTGCCA TAGCCGGCGA CCCCTTCCCC ACTGTGCACT GGCTCCGAGA TGGCAAAGCC
    CTCTCCAGGG ACACTGGCCA CTTCGAGGTG CTACAGAATG AGGACGTGTT CACCCTGGTT
    CTGAAAAACG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGTGTT
    GGCCAATGCA GTTGCCAGGT GTCCCTGATG CTACAGAGCA GCCCGGCCAG AGCGGTCCTG
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC CTCTGTGGCC GAGGAGCTGG TGCTGATGGT
    GGTGGTGGTG GTGACGGCTA CGGGAGCCTG AGGCCTGGCT GGCCGGTGGC GAGAGGGCAA
    GGTGCCCCCG CAGAAGACGA CGGCGAGGAC GTGCGTGGGG TGCTCAAGAG GCGCGTGGAG
    ACACGGCAGC ACACGGAGGA GGCCGTTCGC CAGCAGGAGG TGGAGCAGCT GGACTTCCGC
    GACCTCCTGG GCAAGAAGGT GAGCACCAAG ACCTTGTCTG AAGAGGACCT GAAGGAGATC
    CCAGCCGAGC AGCTGGACTT CCGCGACCTC CTGGGCAAGA AGGTGAGTAC AAAGACCTTG
    TCTGAAGAGG ACCTGAAGGA GATCCCAGCT GAGCAGATGG ATTTCCGCGC CAACCTGCAG
    CGGCAGGTGA AGCCGAAGAC CCTGTCAGAG GAGGAGAGGA AAGTGCATGG CCCCCAGCAG
    GTGGACTTCC GCTCTGTCCT AGCCAAGAAG GGGACTCCCA AGACCCCTGT GCCTGAGAAG
    GTACCCCCTC CAAAACCGGC CACGCCAGAT TTTCGCTCAG TGTTGGGCAG TAAGAAAAAA
    TTACCAACAG AGAATGGTAG CAATAACACT GAGGCCTTGA ATGCCAAGGC AGCAGAAGGT
    CTCAAGCCTG TGGGCAATGC CCAGCCTTCA GGGTTCCTGA AACCCGTGGG CAACGCCAAG
    CTGGCTGATA CCCCCAAACC CTTGAGCAGC ACCAAGCCAG CAGAGACCCC CAAACCCTTG
    GGTAATGTCA AGCCAGCTGA GACCCCCAAA CCCTTGGGTA ATGTCAAGCC AGCTGAGACC
    CCTAAACCTG TGAGCAATGC CAAGCCAGCC GAAACCCTCA AACCTGTGGG CAACGCCAAG
    CCAGCTGAGA CCCCCAAACC CTTGAGTAAC GTCAAGCCAG CTGAAACCCC CAAACTTGTG
    GGCAATGCCA AGCCAGCTGA GACCTCTAAA CCCTTGGATA ACGCCAAGCC AGCTGAGGCC
    CCCAAACCCT TGGGCAATGC CAAGCCGGCT GAAATCCCAA AACCCACTGG GAAAGAAGAA
    CTTAAGAAGG AGATTAAGAA TGATGTGAAC TGCAAGAAGG GCCATGCAGG GGCCACAGAT
    AGTGAAAAGA GACCAGAGAG CCGAGGGACA GCCCCGACCT TCGAGGAGAA ACTACAAGAT
    CTCCATGTGG CAGAGGGTCA GAAGCTGCTG CTCCAGTGCC GGGTGTCCTC TGACCCCCCA
    GCCACCATCA CTTGGACACT GAATGGAAAG ACGCTCAAGA CCACCAAGTT CATCGTCCTC
    TCCCAAGAAG GCTCCTTGTG CTCCGTCTCC ATCGAGAAGG CACTGCCCGA GGACAGAGGC
    TTATACAAGT GTGTAGCCAA GAACAGTGCC GGCCAGGCTG AGTCCTCCTG CCAAGTCACC
    GTGGACGTTC CAGATGCCCC CACCAGTGAA AATGCCAAGG CCCCGGAGAT GAAAGCCCGG
    AGGCCGAAGA GCTCTCTTCC TCCAGTGCTA GGAACAGAAA GTGACGCAAC TGTGAAAAAG
    AAACCTGCCC CCAAGACACC TCCGAAGGCA GCCATGCCGC CTCAGATCAT CCAGTTTCCT
    GAGGACCAGA AGGTGCGAGC AGGAGAGTCT GTGGAGCTCT TTGGTAAGGT GGCCGGCACC
    CAGCCCATCA CCTGCACCTG GATGAAGTTC CGAAAGCAGA TCCAGGACAG CGAGCACATC
    AAGGTGGAGA ACAGCGAGCA GGGCAGCAAG CTCACCATCC GGGCGGCACG CCAGGAGCAC
    TGTGGCTGCT ACACCCTGCT GGTGGAGAAC AAACTGGGCA GCAGGCAGGC CCAAGTCAAC
    CTCACCGTGG TGGACAAGCC AGACCCCCCA GCCGGTACAC CTTGTGCCTC TGACATCCGA
    AGCTCCTCAC TGACCTTGTC CTGGTATGGC TCCTCGTATG ATGGGGGCAG CGCCGTACAG
    TCATACAGTG TCGAGATCTG GGACTCAGTG GACAAGACGT GGAAGGAACT AGCCACGTGC
    CGCAGCACTT CTTTCAACGT CCAGGACCTG CTGCCTGACC GAGAATATAA GTTCCGTGTG
    CGTGCAATCA ATGTCTATGG AACCAGTGAG CCAAGCCAGG AGTCTGAACT CACAGCCTTG
    GGAGAGAAAC CTGAAGAAGA GCCGAAGGAC GAAGTGGAGG TGTCAGATGA TGATGAGAAG
    GAGCCTGAGG TCGACTACCG GACCGTCACG GTCAATACTG AGCAGAAAGT GTCCGACTTC
    TACGACATCG AAGAGAGACT AGGATCTGGG AAATTTGGAC AGGTCTTTCG ACTTGTAGAA
    AAGAAAACTG GAAAAATCTG GGCAGGGAAA TTCTTCAAGG CGTATTCGGC CAAGGAGAAA
    GAGAACATCC GGCAGGAGAT CAGCATCATG AACTGCCTGC ACCACCCCAA GCTGGTCCAG
    TGTGTGGACG CCTTCGAAGA GAAGGCCAAC ATCGTCATGG TCCTGGAGAT CGTGTCCGGG
    GGTGAGCTGT TCGAGCGCAT CATTGATGAG GACTTTGAGC TGACGGAGCG GGAGTGCATC
    AAGTACATGA AGCAGATCTC AGAGGGGGTG GAGTACATCC ACAAGCAGGG CATCGTCCAC
    CTGGACCTCA AGCCCGAGAA CATCATGTGT GTCAACAAGA CAGGCACCAG GATCAAGCTC
    ATCGACTTCG GTCTGGCCAG GAGGTTGGAG AATGCGGGGT CTCTGAAGGT CCTCTTTGGC
    ACCCCGGAGT TCGTCGCACC CGAAGTGATC AACTATGAGC CCATTGGCTA TGCCACGGAC
    ATGTGGAGCA TCGGGGTCAT CTGCTACATC CTGGTCAGTG GACTGTCCCC CTTCATGGGT
    GACAATGACA ACGAAACCTT AGCCAACGTT ACCTCAGCCA CCTGGGACTT TGACGACGAG
    GCCTTCGACG AGATCTCCGA TGACGCCAAG GATTTTATCA GCAACTTGCT GAAGAAGGAT
    ATGAAAAACC GCCTGAACTG CACCCAGTGC CTTCAGCATC CATGGCTAAT GAAAGACACC
    AAGAACATGG AGGCCAAGAA ACTCTCCAAG GACCGGATGA AGAAGTACAT GGCAAGAAGG
    AAATGGCAGA AAACGGGCAA TGCTGTGAGA GCCATCGGAA GACTGTCCTC TATGGCAATG
    ATCTCAGGGC TCAGTGGCAG GAAATCCTCA ACAGGGTCAC CAACCAGCCC GCTCAATGCA
    GAAAAACTAG AATCTGAAGA AGATGTCTCC CAAGCTTTCC TGGAGGCTGT CGCTGAGGAG
    AAGCCCCATG TAAAACCCTA CTTTTCCAAG ACCATTCGTG ACTTAGAAGT TGTGGAGGGA
    AGTGCTGCTA GATTTGACTG CAAGATTGAA GGATACCCGG ACCCTGAAGT CGTCTGGTTC
    AAAGATGACC AATCAATCCG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGATGGG
    AACTGCTCTT TAATCATTAG TGATGTCTGC GGGGATGATG ATGCCAAGTA CACCTGCAAG
    GCTGTCAACA GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATCGTGGA AACCATGGAG
    GAAGGAGAAG GAGAAGGGGG AGAAGAGGAG GAGGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850189.1
    CDS91..6192
    Translation

    Target ORF information:

    RefSeq Version XM_024994421.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT 
    CACTCGAGAG TCAGCTCCAT GCCCCTGACT GAGGCCCCTG CTTTCACTTT GCCCCCTCGG
    AACCTCTGCA TCAAGGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTTCG GGGCTACCCA
    GAGCCCCAAG TGACATGGCA CAGAAACGGA CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGACTGTG GTCCCCGGGG GACTTTCAGC CTTTCGATTC GTGCTGTGCG TGAAGAGGAC
    AAGGGAAAGT ATACCTGTGA AGCCACTAAC GGTAGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGGAGCTT TGTGAAGAAG CCTGGTCAGC CTGTTGTTTC CAAAGCCTTG
    GGGGACAGAT TTGCACCCCC TGCCATGGAG ACCCGTCCTA GCATCTGGGG AGAATGTCCA
    CCAAAATTTG CTACCAAGCT GGGCCGAGCG GTGGTCAGAG AAGGACAGAT GGGGCGGTTC
    TCCTGCAAGA TCACTGGCCG GCCCCAGCCA CAGGTCACCT GGCTCAAGGG AGATGTTCCC
    CTGCAGCCCA GTGCTCGTGT GTCTATGTCT GAGAAGAATG GGATGCAAGT CCTGGAAATC
    CACGACGTCA CCCGAGATGA TGTGGGAGTA TACACGTGCA TGGTGGTGAA CGGGTCAGGG
    AAGGCCTCCA TGTCTGCTGA GCTTTCCATC CAAGGTTTGG ACAATTCCAA TAGGTCACTC
    GTGAGAGGAA CAAAGGTGGC CGGTACGGAC ATCAGGAAGG AAGTGACCAA TGGAGTCACT
    CAGGGGTCCA AACCGGATGG CCTGGATGCT GCTGCCGAGA GTAAGAACCG CCCCAGCACC
    CAGCGAGGCG GCTCCCCGAC CTGGGCCACA GGGAGCCAGC CTCAACCCCC CAGGGAATCT
    GAGCTGGAAC CACTGGGGAA CTCCCCCAGA AAGGGCCAGA GGGCCCCCGT CCTGCAAAAG
    ACCTCCAGCA CCATCACCCT GCAGGCAGCA AAAGTCCAGC TGGAGCCCAG AGCGCCAGTC
    CCAGGCACGC CCTGTCCTTC CAGAGAAGAG ATGGAGGGGC CAGCTGCGTC CCCTCCAGAC
    ACCCTCTCCA CCAGGCGGTC AGGCCTGGGA AGCCAGGACA CCATGGGCAA GGTTGCTACC
    AGGAAGGTCC CCATGGAGAG CCAGAGGGAC ACCATGTTTC CCAAATTTGA GAACAAGCCC
    CAGAGCCAGG AGGTCAGTGA AGATGAGCCA GTCGAGTTCA GATGCGAGGT TTCAGGGATC
    CCAAAACCTG AAGTGACCTG GTTCCTGGAC GGTGTCCCCG TGAGGAGTCA GGAGGGCAGC
    ATCGAGGTTT ACGAAGCTGG GGGGACCCAT TACCTCTGCC TGTGGAGAGC CCGGGCCCGG
    GACAGCGGGA GCTACAGCTG CACAGCCACC AATGTCCGAG GCCAGGCGTC CTGTCGCTGG
    ACGCTCCTGG TGAAAAAGCC AGCCGTGATG GAGGTGGCCC CGTCATTCTC CAGTGCCCTG
    AAGGACTGCA CCGTCGTTGA GGGTCAGGAC TTTGTGCTGC GATGTTCAGT GCAGGGGACC
    CCATTGCCTC GAATCACTTG GCTGCTCAAT GGACAGCCCA TCCAGTACGC CCACTCCACC
    TGCGAGGCTG GCGTGGCCGA GCTCCACATC CAGGACACCC TCCCAGAGGA TGATGGCACC
    TACACCTGTC TGGCTGAGAA CACCGTGGGG CAGGTGTCCT GCAGCGCCCG CGTCACTGTC
    CAGGAGAAGA AGAGTGAGGA GAAGAGCGAG TACCTCCTGC CTGCAGCCCC CAACAAGCCC
    GTCGCGCCCC TCTTCCTGCA GGGCCTCTCT GATCTCCAAG TCATGGATGG GAGCCAGGTC
    ACCATGACAG TCCAGGTGTC AGGAAACCCG CCCCCGGAGG TCATCTGGCT TCACGATGGG
    ACTGAGATCC AGGAGTCCGA GGACTTCCAC TTTGAACAGA GAGGCACTCA GCACAGCCTG
    TGTATCCAGG AAGTGTTCCC AGAGGACACG GGCACTTATA CCTGTGAGGC CTGGAACAGC
    GCAGGAGAGG TCCGCACACA GGCCGTGCTC ACCGTGCAAG AGCCGCAGGA TGGCACCCAG
    CCCTGGTTCA TCAGCAAGCC CCGCTCAGTG ACTGCCTGCC TGGGCCAGAG TGTTCTTATC
    TCCTGTGCCA TAGCCGGCGA CCCCTTCCCC ACTGTGCACT GGCTCCGAGA TGGCAAAGCC
    CTCTCCAGGG ACACTGGCCA CTTCGAGGTG CTACAGAATG AGGACGTGTT CACCCTGGTT
    CTGAAAAACG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGTGTT
    GGCCAATGCA GTTGCCAGGT GTCCCTGATG CTACAGAGCA GCCCGGCCAG AGCGGTCCTG
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC CTCTGTGGCC GAGGAGCTGG TGCTGATGGT
    GGTGGTGGTG GTGACGGCTA CGGGAGCCTG AGGCCTGGCT GGCCGGTGGC GAGAGGGCAA
    GGTGCCCCCG CAGAAGACGA CGGCGAGGAC GTGCGTGGGG TGCTCAAGAG GCGCGTGGAG
    ACACGGCAGC ACACGGAGGA GGCCGTTCGC CAGCAGGAGG TGGAGCAGCT GGACTTCCGC
    GACCTCCTGG GCAAGAAGGT GAGCACCAAG ACCTTGTCTG AAGAGGACCT GAAGGAGATC
    CCAGCCGAGC AGCTGGACTT CCGCGACCTC CTGGGCAAGA AGGTGAGTAC AAAGACCTTG
    TCTGAAGAGG ACCTGAAGGA GATCCCAGCT GAGCAGATGG ATTTCCGCGC CAACCTGCAG
    CGGCAGGTGA AGCCGAAGAC CCTGTCAGAG GAGGAGAGGA AAGTGCATGG CCCCCAGCAG
    GTGGACTTCC GCTCTGTCCT AGCCAAGAAG GGGACTCCCA AGACCCCTGT GCCTGAGAAG
    GTACCCCCTC CAAAACCGGC CACGCCAGAT TTTCGCTCAG TGTTGGGCAG TAAGAAAAAA
    TTACCAACAG AGAATGGTAG CAATAACACT GAGGCCTTGA ATGCCAAGGC AGCAGAAGGT
    CTCAAGCCTG TGGGCAATGC CCAGCCTTCA GGGTTCCTGA AACCCGTGGG CAACGCCAAG
    CTGGCTGATA CCCCCAAACC CTTGAGCAGC ACCAAGCCAG CAGAGACCCC CAAACCCTTG
    GGTAATGTCA AGCCAGCTGA GACCCCCAAA CCCTTGGGTA ATGTCAAGCC AGCTGAGACC
    CCTAAACCTG TGAGCAATGC CAAGCCAGCC GAAACCCTCA AACCTGTGGG CAACGCCAAG
    CCAGCTGAGA CCCCCAAACC CTTGAGTAAC GTCAAGCCAG CTGAAACCCC CAAACTTGTG
    GGCAATGCCA AGCCAGCTGA GACCTCTAAA CCCTTGGATA ACGCCAAGCC AGCTGAGGCC
    CCCAAACCCT TGGGCAATGC CAAGCCGGCT GAAATCCCAA AACCCACTGG GAAAGAAGAA
    CTTAAGAAGG AGATTAAGAA TGATGTGAAC TGCAAGAAGG GCCATGCAGG GGCCACAGAT
    AGTGAAAAGA GACCAGAGAG CCGAGGGACA GCCCCGACCT TCGAGGAGAA ACTACAAGAT
    CTCCATGTGG CAGAGGGTCA GAAGCTGCTG CTCCAGTGCC GGGTGTCCTC TGACCCCCCA
    GCCACCATCA CTTGGACACT GAATGGAAAG ACGCTCAAGA CCACCAAGTT CATCGTCCTC
    TCCCAAGAAG GCTCCTTGTG CTCCGTCTCC ATCGAGAAGG CACTGCCCGA GGACAGAGGC
    TTATACAAGT GTGTAGCCAA GAACAGTGCC GGCCAGGCTG AGTCCTCCTG CCAAGTCACC
    GTGGACGTTC CAGATGCCCC CACCAGTGAA AATGCCAAGG CCCCGGAGAT GAAAGCCCGG
    AGGCCGAAGA GCTCTCTTCC TCCAGTGCTA GGAACAGAAA GTGACGCAAC TGTGAAAAAG
    AAACCTGCCC CCAAGACACC TCCGAAGGCA GCCATGCCGC CTCAGATCAT CCAGTTTCCT
    GAGGACCAGA AGGTGCGAGC AGGAGAGTCT GTGGAGCTCT TTGGTAAGGT GGCCGGCACC
    CAGCCCATCA CCTGCACCTG GATGAAGTTC CGAAAGCAGA TCCAGGACAG CGAGCACATC
    AAGGTGGAGA ACAGCGAGCA GGGCAGCAAG CTCACCATCC GGGCGGCACG CCAGGAGCAC
    TGTGGCTGCT ACACCCTGCT GGTGGAGAAC AAACTGGGCA GCAGGCAGGC CCAAGTCAAC
    CTCACCGTGG TGGACAAGCC AGACCCCCCA GCCGGTACAC CTTGTGCCTC TGACATCCGA
    AGCTCCTCAC TGACCTTGTC CTGGTATGGC TCCTCGTATG ATGGGGGCAG CGCCGTACAG
    TCATACAGTG TCGAGATCTG GGACTCAGTG GACAAGACGT GGAAGGAACT AGCCACGTGC
    CGCAGCACTT CTTTCAACGT CCAGGACCTG CTGCCTGACC GAGAATATAA GTTCCGTGTG
    CGTGCAATCA ATGTCTATGG AACCAGTGAG CCAAGCCAGG AGTCTGAACT CACAGCCTTG
    GGAGAGAAAC CTGAAGAAGA GCCGAAGGAC GAAGTGGAGG TGTCAGATGA TGATGAGAAG
    GAGCCTGAGG TCGACTACCG GACCGTCACG GTCAATACTG AGCAGAAAGT GTCCGACTTC
    TACGACATCG AAGAGAGACT AGGATCTGGG AAATTTGGAC AGGTCTTTCG ACTTGTAGAA
    AAGAAAACTG GAAAAATCTG GGCAGGGAAA TTCTTCAAGG CGTATTCGGC CAAGGAGAAA
    GAGAACATCC GGCAGGAGAT CAGCATCATG AACTGCCTGC ACCACCCCAA GCTGGTCCAG
    TGTGTGGACG CCTTCGAAGA GAAGGCCAAC ATCGTCATGG TCCTGGAGAT CGTGTCCGGG
    GGTGAGCTGT TCGAGCGCAT CATTGATGAG GACTTTGAGC TGACGGAGCG GGAGTGCATC
    AAGTACATGA AGCAGATCTC AGAGGGGGTG GAGTACATCC ACAAGCAGGG CATCGTCCAC
    CTGGACCTCA AGCCCGAGAA CATCATGTGT GTCAACAAGA CAGGCACCAG GATCAAGCTC
    ATCGACTTCG GTCTGGCCAG GAGGTTGGAG AATGCGGGGT CTCTGAAGGT CCTCTTTGGC
    ACCCCGGAGT TCGTCGCACC CGAAGTGATC AACTATGAGC CCATTGGCTA TGCCACGGAC
    ATGTGGAGCA TCGGGGTCAT CTGCTACATC CTGGTCAGTG GACTGTCCCC CTTCATGGGT
    GACAATGACA ACGAAACCTT AGCCAACGTT ACCTCAGCCA CCTGGGACTT TGACGACGAG
    GCCTTCGACG AGATCTCCGA TGACGCCAAG GATTTTATCA GCAACTTGCT GAAGAAGGAT
    ATGAAAAACC GCCTGAACTG CACCCAGTGC CTTCAGCATC CATGGCTAAT GAAAGACACC
    AAGAACATGG AGGCCAAGAA ACTCTCCAAG GACCGGATGA AGAAGTACAT GGCAAGAAGG
    AAATGGCAGA AAACGGGCAA TGCTGTGAGA GCCATCGGAA GACTGTCCTC TATGGCAATG
    ATCTCAGGGC TCAGTGGCAG GAAATCCTCA ACAGGGTCAC CAACCAGCCC GCTCAATGCA
    GAAAAACTAG AATCTGAAGA AGATGTCTCC CAAGCTTTCC TGGAGGCTGT CGCTGAGGAG
    AAGCCCCATG TAAAACCCTA CTTTTCCAAG ACCATTCGTG ACTTAGAAGT TGTGGAGGGA
    AGTGCTGCTA GATTTGACTG CAAGATTGAA GGATACCCGG ACCCTGAAGT CGTCTGGTTC
    AAAGATGACC AATCAATCCG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGATGGG
    AACTGCTCTT TAATCATTAG TGATGTCTGC GGGGATGATG ATGCCAAGTA CACCTGCAAG
    GCTGTCAACA GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATCGTGGA AACCATGGAG
    GAAGGAGAAG GAGAAGGGGG AGAAGAGGAG GAGGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43045
    Clone ID Related Accession (Same CDS sequence) NM_176636.2
    Accession Version NM_176636.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-15
    Organism Bos taurus(cattle)
    Product myosin light chain kinase, smooth muscle
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S57131.1. On or before Oct 28, 2009 this sequence version replaced XM_590786.4, NM_176636.1. ##Evidence-Data-START## Transcript exon combination :: S57131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG 
    AGGAAAGTGC ATGGCCCCCA GCAGGTGGAC TTCCGCTCTG TCCTAGCCAA GAAGGGGACT
    CCCAAGACCC CTGTGCCTGA GAAGGTACCC CCTCCAAAAC CGGCCACGCC AGATTTTCGC
    TCAGTGTTGG GCAGTAAGAA AAAATTACCA ACAGAGAATG GTAGCAATAA CACTGAGGCC
    TTGAATGCCA AGGCAGCAGA AGGTCTCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGGTTC
    CTGAAACCCG TGGGCAACGC CAAGCTGGCT GATACCCCCA AACCCTTGAG CAGCACCAAG
    CCAGCAGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAACGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAACATCAAG
    CCAACTGAGA CCCCCAAACC CTTGGGCAGC ACCAAGCCAG CAGAAACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA GACCCCAAAA CCCTTGGGTA ATGTCAAGCC AGCCGAGACC
    CCCAAACCCT TGGGTAATGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCTAAACC TGTGAGCAAT GCCAAGCCAG CCGAAACCCT CAAACCTGTG
    GGCAACGCCA AGCCAGCTGA GACCCCCAAA CCCTTGAGTA ACGTCAAGCC AGCTGAAACC
    CCCAAACTTG TGGGCAATGC CAAGCCAGCT GAGACCTCTA AACCCTTGGA TAACGCCAAG
    CCAGCTGAGG CCCCCAAACC CTTGGGCAAT GCCAAGCCGG CTGAAATCCC AAAACCCACT
    GGGAAAGAAG AACTTAAGAA GGAGATTAAG AATGATGTGA ACTGCAAGAA GGGCCATGCA
    GGGGCCACAG ATAGTGAAAA GAGACCAGAG AGCCGAGGGA CAGCCCCGAC CTTCGAGGAG
    AAACTACAAG ATCTCCATGT GGCAGAGGGT CAGAAGCTGC TGCTCCAGTG CCGGGTGTCC
    TCTGACCCCC CAGCCACCAT CACTTGGACA CTGAATGGAA AGACGCTCAA GACCACCAAG
    TTCATCGTCC TCTCCCAAGA AGGCTCCTTG TGCTCCGTCT CCATCGAGAA GGCACTGCCC
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAACAGTG CCGGCCAGGC TGAGTCCTCC
    TGCCAAGTCA CCGTGGACGT TCCAGATGCC CCCACCAGTG AAAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC GCCTCAGATC
    ATCCAGTTTC CTGAGGACCA GAAGGTGCGA GCAGGAGAGT CTGTGGAGCT CTTTGGTAAG
    GTGGCCGGCA CCCAGCCCAT CACCTGCACC TGGATGAAGT TCCGAAAGCA GATCCAGGAC
    AGCGAGCACA TCAAGGTGGA GAACAGCGAG CAGGGCAGCA AGCTCACCAT CCGGGCGGCA
    CGCCAGGAGC ACTGTGGCTG CTACACCCTG CTGGTGGAGA ACAAACTGGG CAGCAGGCAG
    GCCCAAGTCA ACCTCACCGT GGTGGACAAG CCAGACCCCC CAGCCGGTAC ACCTTGTGCC
    TCTGACATCC GAAGCTCCTC ACTGACCTTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGCGCCGTAC AGTCATACAG TGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC TTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCGAGAATAT
    AAGTTCCGTG TGCGTGCAAT CAATGTCTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCCT TGGGAGAGAA ACCTGAAGAA GAGCCGAAGG ACGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCTGA GGTCGACTAC CGGACCGTCA CGGTCAATAC CGAGCAGAAA
    GTGTCCGACT TCTACGACAT CGAAGAGAGA CTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TGGAAAAATC TGGGCAGGGA AATTCTTCAA GGCGTATTCG
    GCCAAGGAGA AAGAGAACAT CCGGCAGGAG ATCAGCATCA TGAACTGCCT GCACCACCCC
    AAGCTGGTCC AGTGTGTGGA CGCCTTCGAA GAGAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCCG GGGGTGAGCT GTTCGAGCGC ATCATTGATG AGGACTTTGA GCTGACGGAG
    CGGGAGTGCA TCAAGTACAT GAAGCAGATC TCAGAGGGGG TGGAGTACAT CCACAAGCAG
    GGCATCGTCC ACCTGGACCT CAAGCCCGAG AACATCATGT GTGTCAACAA GACAGGCACC
    AGGATCAAGC TCATCGACTT CGGTCTGGCC AGGAGGTTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCGGA GTTCGTCGCA CCCGAAGTGA TCAACTATGA GCCCATTGGC
    TATGCCACGG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTGGTCAG TGGACTGTCC
    CCCTTCATGG GTGACAATGA CAACGAAACC TTAGCCAACG TTACCTCAGC CACCTGGGAC
    TTTGACGACG AGGCCTTCGA CGAGATCTCC GATGACGCCA AGGATTTTAT CAGCAACTTG
    CTGAAGAAGG ATATGAAAAA CCGCCTGAAC TGCACCCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGACA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATCGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GATGTCTCCC AAGCTTTCCT GGAGGCTGTC
    GCTGAGGAGA AGCCCCATGT AAAACCCTAC TTTTCCAAGA CCATTCGTGA CTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCGGA CCCTGAAGTC
    GTCTGGTTCA AAGATGACCA ATCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGT GATGTCTGCG GGGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATCGTGGAA
    ACCATGGAGG AAGGAGAAGG AGAAGGGGGA GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788809.1
    CDS381..3911
    Translation

    Target ORF information:

    RefSeq Version NM_176636.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin light chain kinase (MYLK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176636.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG 
    AGGAAAGTGC ATGGCCCCCA GCAGGTGGAC TTCCGCTCTG TCCTAGCCAA GAAGGGGACT
    CCCAAGACCC CTGTGCCTGA GAAGGTACCC CCTCCAAAAC CGGCCACGCC AGATTTTCGC
    TCAGTGTTGG GCAGTAAGAA AAAATTACCA ACAGAGAATG GTAGCAATAA CACTGAGGCC
    TTGAATGCCA AGGCAGCAGA AGGTCTCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGGTTC
    CTGAAACCCG TGGGCAACGC CAAGCTGGCT GATACCCCCA AACCCTTGAG CAGCACCAAG
    CCAGCAGAGA CCCCCAAACC CTTGGGTAAT GTCAAGCCAG CTGAGACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA AACCCCCAAA CCCTTGGGCA GCACCAAGCC AGCAGAGACC
    CCAAAACCCT TGGGTAACGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAACATCAAG
    CCAACTGAGA CCCCCAAACC CTTGGGCAGC ACCAAGCCAG CAGAAACCCC CAAACCCTTG
    GGCAGCACCA AGCCAGCAGA GACCCCAAAA CCCTTGGGTA ATGTCAAGCC AGCCGAGACC
    CCCAAACCCT TGGGTAATGT CAAGCCAGCT GAGACCCCCA AACCCTTGGG TAATGTCAAG
    CCAGCTGAGA CCCCTAAACC TGTGAGCAAT GCCAAGCCAG CCGAAACCCT CAAACCTGTG
    GGCAACGCCA AGCCAGCTGA GACCCCCAAA CCCTTGAGTA ACGTCAAGCC AGCTGAAACC
    CCCAAACTTG TGGGCAATGC CAAGCCAGCT GAGACCTCTA AACCCTTGGA TAACGCCAAG
    CCAGCTGAGG CCCCCAAACC CTTGGGCAAT GCCAAGCCGG CTGAAATCCC AAAACCCACT
    GGGAAAGAAG AACTTAAGAA GGAGATTAAG AATGATGTGA ACTGCAAGAA GGGCCATGCA
    GGGGCCACAG ATAGTGAAAA GAGACCAGAG AGCCGAGGGA CAGCCCCGAC CTTCGAGGAG
    AAACTACAAG ATCTCCATGT GGCAGAGGGT CAGAAGCTGC TGCTCCAGTG CCGGGTGTCC
    TCTGACCCCC CAGCCACCAT CACTTGGACA CTGAATGGAA AGACGCTCAA GACCACCAAG
    TTCATCGTCC TCTCCCAAGA AGGCTCCTTG TGCTCCGTCT CCATCGAGAA GGCACTGCCC
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAACAGTG CCGGCCAGGC TGAGTCCTCC
    TGCCAAGTCA CCGTGGACGT TCCAGATGCC CCCACCAGTG AAAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC GCCTCAGATC
    ATCCAGTTTC CTGAGGACCA GAAGGTGCGA GCAGGAGAGT CTGTGGAGCT CTTTGGTAAG
    GTGGCCGGCA CCCAGCCCAT CACCTGCACC TGGATGAAGT TCCGAAAGCA GATCCAGGAC
    AGCGAGCACA TCAAGGTGGA GAACAGCGAG CAGGGCAGCA AGCTCACCAT CCGGGCGGCA
    CGCCAGGAGC ACTGTGGCTG CTACACCCTG CTGGTGGAGA ACAAACTGGG CAGCAGGCAG
    GCCCAAGTCA ACCTCACCGT GGTGGACAAG CCAGACCCCC CAGCCGGTAC ACCTTGTGCC
    TCTGACATCC GAAGCTCCTC ACTGACCTTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGCGCCGTAC AGTCATACAG TGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC TTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCGAGAATAT
    AAGTTCCGTG TGCGTGCAAT CAATGTCTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCCT TGGGAGAGAA ACCTGAAGAA GAGCCGAAGG ACGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCTGA GGTCGACTAC CGGACCGTCA CGGTCAATAC CGAGCAGAAA
    GTGTCCGACT TCTACGACAT CGAAGAGAGA CTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TGGAAAAATC TGGGCAGGGA AATTCTTCAA GGCGTATTCG
    GCCAAGGAGA AAGAGAACAT CCGGCAGGAG ATCAGCATCA TGAACTGCCT GCACCACCCC
    AAGCTGGTCC AGTGTGTGGA CGCCTTCGAA GAGAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCCG GGGGTGAGCT GTTCGAGCGC ATCATTGATG AGGACTTTGA GCTGACGGAG
    CGGGAGTGCA TCAAGTACAT GAAGCAGATC TCAGAGGGGG TGGAGTACAT CCACAAGCAG
    GGCATCGTCC ACCTGGACCT CAAGCCCGAG AACATCATGT GTGTCAACAA GACAGGCACC
    AGGATCAAGC TCATCGACTT CGGTCTGGCC AGGAGGTTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCGGA GTTCGTCGCA CCCGAAGTGA TCAACTATGA GCCCATTGGC
    TATGCCACGG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTGGTCAG TGGACTGTCC
    CCCTTCATGG GTGACAATGA CAACGAAACC TTAGCCAACG TTACCTCAGC CACCTGGGAC
    TTTGACGACG AGGCCTTCGA CGAGATCTCC GATGACGCCA AGGATTTTAT CAGCAACTTG
    CTGAAGAAGG ATATGAAAAA CCGCCTGAAC TGCACCCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGACA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATCGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GATGTCTCCC AAGCTTTCCT GGAGGCTGTC
    GCTGAGGAGA AGCCCCATGT AAAACCCTAC TTTTCCAAGA CCATTCGTGA CTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCGGA CCCTGAAGTC
    GTCTGGTTCA AAGATGACCA ATCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGT GATGTCTGCG GGGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATCGTGGAA
    ACCATGGAGG AAGGAGAAGG AGAAGGGGGA GAAGAGGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.