Gene Symbol | MYLK |
Entrez Gene ID | 338037 |
Full Name | myosin light chain kinase |
Synonyms | MLCK,smMLCK |
Gene Type | protein-coding |
Organism | Bos taurus(cattle) |
ORF » Species Summary » Bos taurus » MYLK cDNA ORF clone
Gene Symbol | MYLK |
Entrez Gene ID | 338037 |
Full Name | myosin light chain kinase |
Synonyms | MLCK,smMLCK |
Gene Type | protein-coding |
Organism | Bos taurus(cattle) |
mRNA | Protein | Name |
---|---|---|
XM_024994475.1 | XP_024850243.1 | myosin light chain kinase, smooth muscle isoform X4 |
XM_024994520.1 | XP_024850288.1 | myosin light chain kinase, smooth muscle isoform X5 |
NM_176636.3 | NP_788809.1 | myosin light chain kinase, smooth muscle |
NM_001372254.1 | NP_001359183.1 | myosin light chain kinase, smooth muscle |
XM_024994569.1 | XP_024850337.1 | myosin light chain kinase, smooth muscle isoform X6 |
NM_176636.2 | NP_788809.1 | myosin light chain kinase, smooth muscle |
XM_024994340.1 | XP_024850108.1 | myosin light chain kinase, smooth muscle isoform X1 |
XM_024994382.1 | XP_024850150.1 | myosin light chain kinase, smooth muscle isoform X2 |
XM_024994421.1 | XP_024850189.1 | myosin light chain kinase, smooth muscle isoform X3 |
A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)
Histamine-induced myosin light chain phosphorylation breaks down the barrier integrity of cultured corneal epithelial cells.
Guo Y, Ramachandran C, Satpathy M, Srinivas SP
Pharmaceutical research24(10)1824-33(2007 Oct)
Isolation of cDNA for bovine stomach 155 kDa protein exhibiting myosin light chain kinase activity.
Kobayashi H, Inoue A, Mikawa T, Kuwayama H, Hotta Y, Masaki T, Ebashi S
Journal of biochemistry112(6)786-91(1992 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Histamine induces myosin light chain (MLC)phosphorylation via MYLK, suggesting regulatory role for MLC in controlling corneal epithelial permeability.
Title: Histamine-induced myosin light chain phosphorylation breaks down the barrier integrity of cultured corneal epithelial cells.
The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OBa218620 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994569.1 | |
Accession Version | XM_024994569.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-05-06 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle isoform X6 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024850337.1 |
CDS | 130..600 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024994569.1 |
1 | ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa218619 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994520.1 | |
Accession Version | XM_024994520.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-05-06 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle isoform X5 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024850288.1 |
CDS | 181..2334 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024994520.1 |
1 | ATGAAAGCCC GGAGGCCGAA GAGCTCTCTT CCTCCAGTGC TAGGAACAGA AAGTGACGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa261971 | |
Clone ID Related Accession (Same CDS sequence) | NM_176636.3 | |
Accession Version | NM_176636.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-26 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Aug 2, 2019 this sequence version replaced NM_176636.2. ##Evidence-Data-START## Transcript exon combination :: S57131.1, SRR5721697.3806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_788809.1 |
CDS | 381..3911 |
Translation |
Target ORF information:
Target ORF information:
|
NM_176636.3 |
1 | ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa218618 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994475.1 , NM_001372254.1 | |
Accession Version | NM_001372254.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-26 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Aug 27, 2019 this sequence version replaced XM_024994475.1. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.47381.1, SRR5571262.32085.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001359183.1 |
CDS | 158..6118 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001372254.1 |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa218618 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994475.1 , NM_001372254.1 | |
Accession Version | XM_024994475.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-05-06 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024850243.1 |
CDS | 152..6112 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024994475.1 |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa218615 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994340.1 | |
Accession Version | XM_024994340.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-05-06 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024850108.1 |
CDS | 165..6554 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024994340.1 |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa218616 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994382.1 | |
Accession Version | XM_024994382.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-05-06 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024850150.1 |
CDS | 96..6482 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024994382.1 |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa218617 | |
Clone ID Related Accession (Same CDS sequence) | XM_024994421.1 | |
Accession Version | XM_024994421.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-05-06 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024850189.1 |
CDS | 91..6192 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024994421.1 |
1 | ATGGGGGATG TGAAGCTGGC CGCCCAGACA CACGTTTCCA AAACTTCCCT CACTGTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OBa43045 | |
Clone ID Related Accession (Same CDS sequence) | NM_176636.2 | |
Accession Version | NM_176636.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-15 | |
Organism | Bos taurus(cattle) | |
Product | myosin light chain kinase, smooth muscle | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S57131.1. On or before Oct 28, 2009 this sequence version replaced XM_590786.4, NM_176636.1. ##Evidence-Data-START## Transcript exon combination :: S57131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_788809.1 |
CDS | 381..3911 |
Translation |
Target ORF information:
Target ORF information:
|
NM_176636.2 |
1 | ATGGATTTCC GCGCCAACCT GCAGCGGCAG GTGAAGCCGA AGACCCTGTC AGAGGAGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
Histamine-induced myosin light chain phosphorylation breaks down the barrier integrity of cultured corneal epithelial cells. |
Isolation of cDNA for bovine stomach 155 kDa protein exhibiting myosin light chain kinase activity. |