CCNB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCNB1
Entrez Gene ID	327679
Full Name	cyclin B1
General protein information	Preferred Names cyclin B1 Names G2/mitotic-specific cyclin-B1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045872.1	NP_001039337.1	G2/mitotic-specific cyclin-B1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ccnb1	NP_571588.1
Canis lupus familiaris (dog)	CCNB1	XP_850398.1
Rattus norvegicus (Norway rat)	Ccnb1	NP_741988.1
Saccharomyces cerevisiae (baker's yeast)	CLB3	NP_010126.1
Arabidopsis thaliana (thale cress)	CYC1BAT	NP_196233.1
	CCNB1	XP_001091533.1
Mus musculus (house mouse)	Ccnb1	NP_758505.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004962	XP_315059.4
Homo sapiens (human)	CCNB1	NP_114172.1
Pan troglodytes (chimpanzee)	CCNB1	XP_517728.3
Bos taurus (cattle)	CCNB1	NP_001039337.1
Drosophila melanogaster (fruit fly)	CycB	NP_726244.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004963	XP_315060.4
Mus musculus (house mouse)	Gm5593	XP_485921.1
Arabidopsis thaliana (thale cress)	CYCB1;3	NP_187759.2
Arabidopsis thaliana (thale cress)	CYCB1;1	NP_195465.1
Xenopus tropicalis (tropical clawed frog)	ccnb1.2	NP_989121.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of cyclin-dependent kinase (CDK) inhibition on expression, localization and activity of maturation promoting factor (MPF) and mitogen activated protein kinase (MAPK) in bovine oocytes.
Quetglas MD, Adona PR, de Bem TH, Pires PR, Leal CL
Reproduction in domestic animals = Zuchthygiene45(6)1074-81(2010 Dec)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Effects of polyadenylation inhibition on meiosis progression in relation to the polyadenylation status of cyclins A2 and B1 during in vitro maturation of bovine oocytes.
Traverso JM, Donnay I, Lequarre AS
Molecular reproduction and development71(1)107-14(2005 May)

Expression of cyclin B1 messenger RNA isoforms and initiation of cytoplasmic polyadenylation in the bovine oocyte.
Tremblay K, Vigneault C, McGraw S, Sirard MA
Biology of reproduction72(4)1037-44(2005 Apr)

Identification of cDNAs encoding bovine cyclin B and Cdk1/Cdc2.
Yang L, Farin CE
Gene141(2)283-6(1994 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cyclin-dependent kinase inhibition did not affect the expression (mRNA and protein levels) and localization of maturation promoting factor(MPF) and MAPK, and had nearly no effect on kinase activities during maturation.
Title: Effect of cyclin-dependent kinase (CDK) inhibition on expression, localization and activity of maturation promoting factor (MPF) and mitogen activated protein kinase (MAPK) in bovine oocytes.

Polyadenylation of cyclin B1 was also completely prevented when 3'-dA was added at 0 hr, and greatly reduced when added at 6 hr
Title: Effects of polyadenylation inhibition on meiosis progression in relation to the polyadenylation status of cyclins A2 and B1 during in vitro maturation of bovine oocytes.

link between cytoplasmic polyadenylation of cyclin B1 and translation/appearance of cyclin B1 protein before in vitro maturation of oocytes
Title: Expression of cyclin B1 messenger RNA isoforms and initiation of cytoplasmic polyadenylation in the bovine oocyte.

The following CCNB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCNB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42096	NM_001045872.1 Latest version!	Bos taurus cyclin B1 (CCNB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCNB1 ( NM_001045872.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039337.1 Bos taurus cyclin B1 (CCNB1), mRNA.

Price & Availability↑

CloneID	OBa42096
Clone ID Related Accession (Same CDS sequence)	NM_001045872.1
Accession Version	NM_001045872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	G2/mitotic-specific cyclin-B1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC116011.1. On or before Sep 7, 2006 this sequence version replaced XM_864391.1, XM_873085.1, XM_873185.1, XM_590022.2. ##Evidence-Data-START## Transcript exon combination :: BC116011.1, L26548.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCTCC GGATCACCAG GAACACGAAG ATTAGTGCTG AAAATAAGGC GAAGATCAGT 
ATGGCAGGGG CCAAGCGCGT GCCTGTGGCC GCTGTTGCAA CATCTAAGCC CGGGCTGAGG 
CCCCGAACAG CTCTTGGAGA CATCGGTAAC AAAGTCAGTG AACAACCGCA GGCCAAACTG 
CCTCTGAAAA AGGAAGCAAA AACTTTAGCT TCTGGAAAAG TTACTGCTAA AAAAGTACCA 
AAACCTCTGG AAAAGGCTCC TGTACCTGTG CCGGAGCCCC AGCCGGAGCC AGAGCCGGAA 
CCCGAGCATG TTAAGGAAGA CAAACTTTCC CCCGAGCCTA TTTTGGTCGA TACTCCCTCT 
CCAAGCCCCA TGGAAACATC TGGCTGTGCC CCTGCAGAAG AGTATCTGTG TCAGGCTTTC 
TCAGATGTAA TTCTTGCAGT GAGTGATGTG GATGCAGAAG ACGGAGCGGA TCCAAACCTT 
TGTAGTGAAT ATGTAAAAGA TATCTATGCT TATCTAAGAC AACTTGAGGA AGAGCAAGCA 
GTCAAACCAA AATACCTAAT GGGTCGTGAA GTCACTGGAA ACATGAGAGC CATCCTCATT 
GACTGGCTAG TGCAAGTTCA AATAAAATTC AGATTACTGC AGGAGACCAT GTACATGACT 
GTTTCCATAA TTGATCGGTT CATGCAGGAT ACCTATGTGC CCAAGAAGAT GCTGCAGCTG 
GTTGGAGTCA CTGCCATGTT TGTTGCAAGC AAATATGAGG AAATGTACCC TCCAGAAATC 
GGTGACTTTG CCTTTGTGAC TGACAACACC TACACCAAGT TTCAAATCAG ACAGATGGAA 
ATGAAGATTC TAAGAGCTTT AAATTTTAGT CTGGGTCGCC CTCTACCCCT GCATTTCCTT 
CGGAGAGCAT CTAAGATTGG AGAGGTTGAT GTTGAGCTAC ATACTCTGGC CAAATATTTG 
ATGGAACTAA CTATGCTGGA CTACGATATG GTGCACTTTC CTCCTTCTCA GATTGCAGCG 
GGAGCTTTTT GCTTAGCACT GAAAGTTCTT GATAATGGTG AATGGACACC AACTCTACAG 
CATTACCTGT CATATACTGA AGAATCCCTT CTCGTTGTTA TGCAACACCT GGCAAAGAAT 
GTGGTCATGG TGAACCGTGG GCTCACAAAG CACATGACTA TCAAGAACAA GTATGCCACA 
TCTAAGCATG CTAAGATCAG CACTCTAGCA CAGCTGAATT CTGCACTAGT TCAAGATTTA 
GCCAAGGCTG TGGCAAAGGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039337.1
CDS	75..1358
Translation	MALRITRNTKISAENKAKISMAGAKRVPVAAVATSKPGLRPRTALGDIGNKVSEQPQAKLPLKKEAKTLASGKVTAKKVPKPLEKAPVPVPEPQPEPEPEPEHVKEDKLSPEPILVDTPSPSPMETSGCAPAEEYLCQAFSDVILAVSDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVKPKYLMGREVTGNMRAILIDWLVQVQIKFRLLQETMYMTVSIIDRFMQDTYVPKKMLQLVGVTAMFVASKYEEMYPPEIGDFAFVTDNTYTKFQIRQMEMKILRALNFSLGRPLPLHFLRRASKIGEVDVELHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKVLDNGEWTPTLQHYLSYTEESLLVVMQHLAKNVVMVNRGLTKHMTIKNKYATSKHAKISTLAQLNSALVQDLAKAVAKV

Target ORF information:

RefSeq Version	NM_001045872.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclin B1 (CCNB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045872.1

ORF Insert Sequence:

ATGGCGCTCC GGATCACCAG GAACACGAAG ATTAGTGCTG AAAATAAGGC GAAGATCAGT 
ATGGCAGGGG CCAAGCGCGT GCCTGTGGCC GCTGTTGCAA CATCTAAGCC CGGGCTGAGG 
CCCCGAACAG CTCTTGGAGA CATCGGTAAC AAAGTCAGTG AACAACCGCA GGCCAAACTG 
CCTCTGAAAA AGGAAGCAAA AACTTTAGCT TCTGGAAAAG TTACTGCTAA AAAAGTACCA 
AAACCTCTGG AAAAGGCTCC TGTACCTGTG CCGGAGCCCC AGCCGGAGCC AGAGCCGGAA 
CCCGAGCATG TTAAGGAAGA CAAACTTTCC CCCGAGCCTA TTTTGGTCGA TACTCCCTCT 
CCAAGCCCCA TGGAAACATC TGGCTGTGCC CCTGCAGAAG AGTATCTGTG TCAGGCTTTC 
TCAGATGTAA TTCTTGCAGT GAGTGATGTG GATGCAGAAG ACGGAGCGGA TCCAAACCTT 
TGTAGTGAAT ATGTAAAAGA TATCTATGCT TATCTAAGAC AACTTGAGGA AGAGCAAGCA 
GTCAAACCAA AATACCTAAT GGGTCGTGAA GTCACTGGAA ACATGAGAGC CATCCTCATT 
GACTGGCTAG TGCAAGTTCA AATAAAATTC AGATTACTGC AGGAGACCAT GTACATGACT 
GTTTCCATAA TTGATCGGTT CATGCAGGAT ACCTATGTGC CCAAGAAGAT GCTGCAGCTG 
GTTGGAGTCA CTGCCATGTT TGTTGCAAGC AAATATGAGG AAATGTACCC TCCAGAAATC 
GGTGACTTTG CCTTTGTGAC TGACAACACC TACACCAAGT TTCAAATCAG ACAGATGGAA 
ATGAAGATTC TAAGAGCTTT AAATTTTAGT CTGGGTCGCC CTCTACCCCT GCATTTCCTT 
CGGAGAGCAT CTAAGATTGG AGAGGTTGAT GTTGAGCTAC ATACTCTGGC CAAATATTTG 
ATGGAACTAA CTATGCTGGA CTACGATATG GTGCACTTTC CTCCTTCTCA GATTGCAGCG 
GGAGCTTTTT GCTTAGCACT GAAAGTTCTT GATAATGGTG AATGGACACC AACTCTACAG 
CATTACCTGT CATATACTGA AGAATCCCTT CTCGTTGTTA TGCAACACCT GGCAAAGAAT 
GTGGTCATGG TGAACCGTGG GCTCACAAAG CACATGACTA TCAAGAACAA GTATGCCACA 
TCTAAGCATG CTAAGATCAG CACTCTAGCA CAGCTGAATT CTGCACTAGT TCAAGATTTA 
GCCAAGGCTG TGGCAAAGGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of cyclin-dependent kinase (CDK) inhibition on expression, localization and activity of maturation promoting factor (MPF) and mitogen activated protein kinase (MAPK) in bovine oocytes.
Reproduction in domestic animals = Zuchthygiene45(6)1074-81(2010 Dec)
Quetglas MD,Adona PR,de Bem TH,Pires PR,Leal CL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Effects of polyadenylation inhibition on meiosis progression in relation to the polyadenylation status of cyclins A2 and B1 during in vitro maturation of bovine oocytes.
Molecular reproduction and development71(1)107-14(2005 May)
Traverso JM,Donnay I,Lequarre AS

Expression of cyclin B1 messenger RNA isoforms and initiation of cytoplasmic polyadenylation in the bovine oocyte.
Biology of reproduction72(4)1037-44(2005 Apr)
Tremblay K,Vigneault C,McGraw S,Sirard MA

Identification of cDNAs encoding bovine cyclin B and Cdk1/Cdc2.
Gene141(2)283-6(1994 Apr)
Yang L,Farin CE