NDUFS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFS1
Entrez Gene ID	288380
Full Name	NADH:ubiquinone oxidoreductase core subunit S1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005202713.4	XP_005202770.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
XM_024999193.1	XP_024854961.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
NM_174820.4	NP_777245.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
XM_005202713.3	XP_005202770.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
XM_024999195.1	XP_024854963.1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ndufs1	NP_001007766.1
Drosophila melanogaster (fruit fly)	ND75	NP_511083.1
Arabidopsis thaliana (thale cress)	EMB1467	NP_568550.1
Oryza sativa (rice)	Os03g0713400	NP_001051072.1
Xenopus tropicalis (tropical clawed frog)	ndufs1	XP_002936432.2
Neurospora crassa	NCU01765	XP_957188.2
Homo sapiens (human)	NDUFS1	NP_001186913.1
	NDUFS1	XP_001105816.2
Rattus norvegicus (Norway rat)	Ndufs1	NP_001005550.1
Caenorhabditis elegans (roundworm)	nuo-5	NP_503733.1
Canis lupus familiaris (dog)	NDUFS1	XP_005640630.1
Magnaporthe oryzae (rice blast fungus)	MGG_03155	XP_003716838.1
Pan troglodytes (chimpanzee)	NDUFS1	NP_001073384.1
Bos taurus (cattle)	NDUFS1	NP_777245.1
Mus musculus (house mouse)	Ndufs1	NP_001153510.1
Gallus gallus (chicken)	NDUFS1	NP_001006518.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001653	XP_003435895.1

Gene Ontology
Bibliography

Related articles in PubMed

New insights into the superoxide generation sites in bovine heart NADH-ubiquinone oxidoreductase (Complex I): the significance of protein-associated ubiquinone and the dynamic shifting of generation sites between semiflavin and semiquinone radicals.
Ohnishi ST, Shinzawa-Itoh K, Ohta K, Yoshikawa S, Ohnishi T
Biochimica et biophysica acta1797(12)1901-9(2010 Dec)

Possible roles of two quinone molecules in direct and indirect proton pumps of bovine heart NADH-quinone oxidoreductase (complex I).
Ohnishi ST, Salerno JC, Ohnishi T
Biochimica et biophysica acta1797(12)1891-3(2010 Dec)

Bovine heart NADH-ubiquinone oxidoreductase contains one molecule of ubiquinone with ten isoprene units as one of the cofactors.
Shinzawa-Itoh K, Seiyama J, Terada H, Nakatsubo R, Naoki K, Nakashima Y, Yoshikawa S
Biochemistry49(3)487-92(2010 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusov? E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that during NADH-ubiquinone oxidoreductase (Complex I) catalytic electron transfer from NADH to DBQ, the superoxide generation site was mostly shifted to the SQ.
Title: New insights into the superoxide generation sites in bovine heart NADH-ubiquinone oxidoreductase (Complex I): the significance of protein-associated ubiquinone and the dynamic shifting of generation sites between semiflavin and semiquinone radicals.

Data suggest that Q(Nf) plays a role in a "direct" redox-driven proton pump, while Q(Ns) triggers an "indirect" conformation-driven proton pump in NADH-ubiquinone oxidoreductase (complex I).
Title: Possible roles of two quinone molecules in direct and indirect proton pumps of bovine heart NADH-quinone oxidoreductase (complex I).

Bovine heart NADH-ubiquinone oxidoreductase contains one molecule of ubiquinone with ten isoprene units as one of the cofactors
Title: Bovine heart NADH-ubiquinone oxidoreductase contains one molecule of ubiquinone with ten isoprene units as one of the cofactors.

The following NDUFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01304	NM_174820.4 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01304	XM_024999193.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01304	XM_024999195.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01304	XM_005202713.4 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa01304	XM_005202713.3	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFS1 ( NM_174820.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777245.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), mRNA.

Price & Availability↑

CloneID	OBa01304
Clone ID Related Accession (Same CDS sequence)	XM_005202713.4 , XM_024999193.1 , NM_174820.4 , XM_005202713.3 , XM_024999195.1
Accession Version	NM_174820.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC120069.1. On Aug 31, 2012 this sequence version replaced NM_174820.3. ##Evidence-Data-START## Transcript exon combination :: BC120069.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777245.1
CDS	131..2314
Translation	MLRIPVRKALVGLSKSSKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGSSALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHAVEEPSIC

Target ORF information:

RefSeq Version	NM_174820.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174820.4

ORF Insert Sequence:

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS1 ( XM_005202713.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202770.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01304
Clone ID Related Accession (Same CDS sequence)	XM_005202713.4 , XM_024999193.1 , NM_174820.4 , XM_005202713.3 , XM_024999195.1
Accession Version	XM_005202713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202770.1
CDS	147..2330
Translation	MLRIPVRKALVGLSKSSKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGSSALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHAVEEPSIC

Target ORF information:

RefSeq Version	XM_005202713.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202713.4

ORF Insert Sequence:

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS1 ( XM_024999193.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854961.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa01304
Clone ID Related Accession (Same CDS sequence)	XM_005202713.4 , XM_024999193.1 , NM_174820.4 , XM_005202713.3 , XM_024999195.1
Accession Version	XM_024999193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854961.1
CDS	186..2369
Translation	MLRIPVRKALVGLSKSSKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGSSALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHAVEEPSIC

Target ORF information:

RefSeq Version	XM_024999193.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999193.1

ORF Insert Sequence:

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS1 ( XM_005202713.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202770.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01304
Clone ID Related Accession (Same CDS sequence)	XM_005202713.4 , XM_024999193.1 , NM_174820.4 , XM_005202713.3 , XM_024999195.1
Accession Version	XM_005202713.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202770.1
CDS	130..2313
Translation	MLRIPVRKALVGLSKSSKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGSSALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHAVEEPSIC

Target ORF information:

RefSeq Version	XM_005202713.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202713.3

ORF Insert Sequence:

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFS1 ( XM_024999195.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854963.1 Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa01304
Clone ID Related Accession (Same CDS sequence)	XM_005202713.4 , XM_024999193.1 , NM_174820.4 , XM_005202713.3 , XM_024999195.1
Accession Version	XM_024999195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854963.1
CDS	82..2265
Translation	MLRIPVRKALVGLSKSSKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVAGMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGSSALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHAVEEPSIC

Target ORF information:

RefSeq Version	XM_024999195.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999195.1

ORF Insert Sequence:

ATGTTAAGGA TACCTGTAAG AAAGGCCTTA GTAGGCCTTT CCAAGTCTTC TAAAGGATGT 
GTTCGAACAA CTGCCACAGC AGCAAGCAAC TTGATTGAAG TATTTGTTGA TGGTCAGTCT 
GTCATGGTGG AACCAGGAAC TACCGTCCTT CAAGCTTGTG AGAAGGTTGG CATGCAGATT 
CCTCGATTCT GTTATCACGA AAGGTTGTCT GTTGCTGGAA ACTGCAGGAT GTGCCTTGTT 
GAAATTGAGA AAGCTCCTAA GGTTGTAGCT GCTTGTGCCA TGCCAGTAAT GAAAGGTTGG 
AATATCCTGA CAAACTCTGA GAAAACTAAG AAAGCCAGAG AAGGTGTGAT GGAGTTCTTA 
TTAGCAAATC ACCCACTGGA CTGTCCTATT TGTGACCAGG GAGGTGAATG TGATCTGCAG 
GACCAGTCCA TGATGTTTGG AAGTGATAGG AGCCGGTTTT TAGAGGGGAA ACGTGCTGTG 
GAGGACAAGA ACATTGGGCC GTTGGTAAAA ACCATTATGA CTAGGTGTAT ACAGTGTACT 
CGCTGCATCA GGTTTGCAAG TGAGATCGCA GGAGTGGATG ATTTGGGAAC TACAGGCAGA 
GGAAACGATA TGCAAGTTGG CACATACATT GAAAAGATGT TCATGTCTGA ACTTTCTGGC 
AATATCATTG ACATCTGCCC TGTAGGTGCC CTGACTTCTA AGCCCTATGC CTTTACCGCT 
CGGCCTTGGG AAACAAGAAA GACAGAATCC ATTGATGTAA TGGATGCAGT TGGAAGTAAT 
ATTGTGGTCA GCACAAGAAC TGGAGAGGTA ATGAGGATTT TGCCAAGGAT GCATGAGGAC 
ATCAATGAAG AGTGGATCTC TGATAAAACC AGATTTGCCT ATGATGGGCT AAAACGTCAA 
AGACTTACCG AACCGATGGT CAGAAATGAA AAGGGACTTT TAACACATAC CACCTGGGAG 
GATGCACTCT CTCGTGTAGC TGGAATGTTG CAGAGCTTTC AAGGCAATGA TGTGGCAGCA 
ATTGCAGGTG GCTTGGTGGA TGCTGAAGCC CTCATAGCTC TCAAAGATTT ACTTAATAGA 
GTGGATTCTG ACACGCTGTG CACTGAAGAG GTCTTCCCCA CCGCAGGAGC TGGCACAGAT 
CTACGTTCCA ATTATCTTCT TAATACTACA ATCGCTGGTG TGGAGGAGGC AGATGTTGTC 
CTTCTAGTTG GTACAAATCC ACGTTTTGAG GCACCGCTAT TTAATGCTAG AATTCGAAAG 
AGCTGGCTTC ATAATGACTT AAAAGTGGCC CTTATAGGCA GCCCAGTGGA TCTCACTTAC 
AGATATGACC ATCTGGGAGA TTCTCCCAAA ATTCTTCAAG ACATTGCTTC GGGTAGCCAT 
CCATTCAGCC AGGTCCTACA GGAAGCTAAA AAACCAATGG TGATTTTAGG CAGTTCTGCA 
CTCCAAAGAA ATGATGGTGC AGCAATTCTT GCAGCTGTTT CCAACATTGC TCAGAAGATT 
CGGACAAGTA GTGGTGTTAC TGGTGATTGG AAAGTTATGA ATATCCTTCA TAGGATTGCA 
AGCCAAGTAG CTGCTTTGGA TCTTGGCTAT AAGCCTGGGG TGGAAGCAAT TCAGAAGAAT 
CCTCCTAAAA TGCTGTTTCT CCTGGGAGCA GATGGAGGTT GTATCACTCG ACAGGATTTG 
CCAAAGGATT GTTTCATTGT TTATCAAGGA CATCATGGCG ACGTCGGAGC TCCTATAGCT 
GATGTTATTC TCCCTGGCGC TGCTTACACA GAGAAGTCTG CTACTTACGT CAATACCGAG 
GGCCGAGCTC AGCAGACAAA AGTAGCAGTG ACGCCTCCCG GCTTGGCAAG AGAAGACTGG 
AAAATTATAA GAGCCCTTTC TGAGATTGCA GGTATGACTC TTCCATATGA TACTCTGGAT 
CAAGTGAGAA ACAGATTGGA AGAAGTCTCT CCTAATCTTG TTCGATATGA TGATGTTGAA 
GGAGCTAATT ATTTCCAGCA AGCAAGTGAG CTTTCCAAGC TAGTGAACCA ACAGCTTCTT 
GCTGATCCAC TTGTTCCACC TCAGCTAACA ATAAAAGACT TCTACATGAC AGATTCAATT 
AGCAGAGCCT CACAGACAAT GGCTAAATGT GTCAAAGCTG TTACAGAGGG TGCCCATGCA 
GTAGAGGAAC CATCCATATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

New insights into the superoxide generation sites in bovine heart NADH-ubiquinone oxidoreductase (Complex I): the significance of protein-associated ubiquinone and the dynamic shifting of generation sites between semiflavin and semiquinone radicals.
Biochimica et biophysica acta1797(12)1901-9(2010 Dec)
Ohnishi ST,Shinzawa-Itoh K,Ohta K,Yoshikawa S,Ohnishi T

Possible roles of two quinone molecules in direct and indirect proton pumps of bovine heart NADH-quinone oxidoreductase (complex I).
Biochimica et biophysica acta1797(12)1891-3(2010 Dec)
Ohnishi ST,Salerno JC,Ohnishi T

Bovine heart NADH-ubiquinone oxidoreductase contains one molecule of ubiquinone with ten isoprene units as one of the cofactors.
Biochemistry49(3)487-92(2010 Jan)
Shinzawa-Itoh K,Seiyama J,Terada H,Nakatsubo R,Naoki K,Nakashima Y,Yoshikawa S

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusov? E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

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NDUFS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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