GPLD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPLD1
Entrez Gene ID	287025
Full Name	glycosylphosphatidylinositol specific phospholipase D1
General protein information	Preferred Names glycosylphosphatidylinositol specific phospholipase D1 Names phosphatidylinositol-glycan-specific phospholipase D GPI-PLD GPI-specific phospholipase D PI-G PLD glycoprotein phospholipase D glycosyl-phosphatidylinositol-specific phospholipase D
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015459945.1	XP_015315431.1	phosphatidylinositol-glycan-specific phospholipase D isoform X1
NM_174816.2	NP_777241.1	phosphatidylinositol-glycan-specific phospholipase D precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPLD1	XP_518268.4
Xenopus tropicalis (tropical clawed frog)	LOC100493339	XP_002937968.2
Homo sapiens (human)	GPLD1	NP_001494.2
	GPLD1	XP_002803682.1
Canis lupus familiaris (dog)	GPLD1	XP_535902.2
Bos taurus (cattle)	GPLD1	NP_777241.1
Mus musculus (house mouse)	Gpld1	NP_032182.2
Rattus norvegicus (Norway rat)	Gpld1	NP_001093982.1
Gallus gallus (chicken)	GPLD1	XP_418910.4

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Phospholipase cleavage of D- and L-chiro-glycosylphosphoinositides asymmetrically incorporated into liposomal membranes.
Bonilla JB, Cid MB, Contreras FX, Go?i FM, Mart?n-Lomas M
Chemistry (Weinheim an der Bergstrasse, Germany)12(5)1513-28(2006 Feb)

Phosphorylation of glycosyl-phosphatidylinositol by phosphatidylinositol 3-kinase changes its properties as a substrate for phospholipases.
Jones DR, Pa?eda C, Villar AV, Alonso A, Go?i FM, B?tikofer P, Brodbeck U, Shepherd PR, Varela-Nieto I
FEBS letters579(1)59-65(2005 Jan)

Hyperoxia alters phorbol ester-induced phospholipase D activation in bovine lung microvascular endothelial cells.
Roy S, Parinandi N, Zeigelstein R, Hu Q, Pei Y, Travers JB, Natarajan V
Antioxidants & redox signaling5(2)217-28(2003 Apr)

Purification and characterization of glycosyl-phosphatidylinositol-specific phospholipase D.
Huang KS, Li S, Fung WJ, Hulmes JD, Reik L, Pan YC, Low MG
The Journal of biological chemistry265(29)17738-45(1990 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLD plays an important role in inflammatory responses and could be involved in a mechanism for the regulation of endothelial barrier function during hyperoxic lung injury
Title: Hyperoxia alters phorbol ester-induced phospholipase D activation in bovine lung microvascular endothelial cells.

The following GPLD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPLD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41793	NM_174816.2 Latest version!	Bos taurus glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa32230	XM_015459945.1 Latest version!	Bos taurus glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPLD1 ( NM_174816.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777241.1 Bos taurus glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Price & Availability↑

CloneID	OBa41793
Clone ID Related Accession (Same CDS sequence)	NM_174816.2
Accession Version	NM_174816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	phosphatidylinositol-glycan-specific phospholipase D precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M60804.1. On Jun 3, 2003 this sequence version replaced NM_174816.1. ##Evidence-Data-START## Transcript exon combination :: M60804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCTT TCAGATTCTG GTCAGGACTG CTGATGCTAC TGGGCTTCCT CTGCCCTAGA 
AGTTCACCAT GTGGCATTTC GACACACATA GAAATAGGAC ACAGAGCTCT GGAGTTTCTC 
CACCTTCAGG ATGGGAGTAT TAACTACAAA GAGCTGTTAC TTAGGCACCA GGATGCATAT 
CAGGCTGGAT CCGTGTTTCC TGACTCATTT TACCCTAGCA TCTGTGAGAG AGGACAATTC 
CATGACGTGT CAGAGAGCAC TCACTGGACT CCATTTCTTA ACGCAAGTGT TCATTATATC 
CGGAAGAACT ATCCTCTTCC CTGGGATGAG GACACAGAGA AATTGGTAGC TTTCTTGTTT 
GGAATTACGT CTCACATGGT GGCTGATGTC AACTGGCATA GCCTGGGTAT TGAACAAGGA 
TTCCTTAGGA CGATGGCTGC CATTGATTTT CACAACTCCT ATCCCGAGGC ACATCCGGCT 
GGTGATTTCG GAGGAGACGT GTTGAGCCAG TTCGAGTTTA AATTTAATTA CCTCTCACGG 
CACTGGTATG TGCCTGCTGA AGATCTCCTG GGAATTTATA GAGAACTCTA CGGCCGAATA 
GTCATCACCA AAAAAGCCAT TGTTGACTGT TCATACCTTC AATTCTTGGA AATGTATGCG 
GAGATGTTAG CTATTTCCAA GCTTTATCCC ACTTATTCTG TAAAATCCCC ATTTTTGGTG 
GAACAATTTC AAGAATACTT CCTAGGAGGG CTGGAAGATA TGGCGTTTTG GTCCACTAAT 
ATTTACCATC TGACAAGTTA CATGTTAAAG AACGGGACCA GTAACTGCAA CCTCCCTGAG 
AACCCTCTGT TCATCACATG TGGCGGTCAA CAAAACAACA CCCATGGCTC AAAAGTACAG 
AAAAATGGTT TTCATAAAAA TGTGACTGCA GCCCTAACTA AAAATATTGG AAAGCATATA 
AACTATACCA AAAGAGGAGT GTTCTTTAGT GTGGATTCCT GGACCATGGA TTCCTTATCC 
TTCATGTACA AGTCTTTGGA GAGGAGTATA CGGGAGATGT TTATTGGCAG CTCTCAGCCA 
CTGACACATG TTTCTAGCCC CGCAGCATCT TACTACTTGT CATTTCCCTA CACAAGGCTT 
GGTTGGGCAA TGACTTCAGC TGATCTCAAC CAGGATGGAT ACGGTGACCT GGTGGTGGGT 
GCCCCTGGCT ACAGCCACCC AGGCCGCATT CACGTGGGGC GCGTGTACCT CATCTATGGC 
AATGACCTGG GCTTGCCCCG TATCGACCTG GACCTGGACA AGGAGGCCCA CGGGATCCTG 
GAGGGTTTCC AGCCCTCAGG TCGATTTGGC TCGGCTGTGG CTGTGCTAGA CTTTAACGTG 
GATGGCGTGC CTGACCTGGC GGTGGGAGCC CCCTCGGTGG GCTCCGAGAA GCTCACATAC 
ACAGGTGCAG TGTATGTCTA CTTCGGTTCC AAACAAGGAC AACTATCTTC TTCCCCCAAC 
GTCACCATCT CTTGCCAGGA TACCTACTGT AACTTGGGCT GGACCCTCCT GGCGGCAGAT 
GTGAATGGAG ATAGTGAACC GGACCTGGTG ATTGGCTCCC CTTTTGCTCC AGGTGGAGGG 
AAACAGAAGG GAATTGTGGC TGCATTTTAC TCTGGCTCCA GTTACAGCAG CCGAGAAAAG 
CTGAATGTGG AGGCTGCCAA CTGGATGGTG AAAGGCGAGG AGGACTTTGC TTGGTTGGGG 
TACTCCCTTC ACGGTGTCAA TGTCAACAAC AGGACTTTGC TCCTGGCTGG AAGCCCGACC 
TGGAAGGACA CCAGTAGTCA GGGCCACTTG TTCCGCACTC GTGATGAGAA ACAGAGCCCT 
GGACGGGTGT ACGGCTATTT CCCGCCAATC TGTCAAAGCT GGTTTACCAT TTCCGGAGAC 
AAGGCAATGG GGAAACTGGG TACCTCCCTG TCTAGTGGCC ACGTGATGGT GAACGGGACC 
CGGACCCAAG TGCTGCTGGT GGGGGCCCCG ACTCAAGATG TCGTGTCTAA GGTATCATTC 
CTGACCATGA CCCTGCACCA AGGTGGGAGC ACTCGGATGT ATGAACTGAC ACCTGACTCA 
CAGCCTTCTC TGCTCAGCAC CTTCAGTGGA AACCGCCGCT TCTCCCGATT TGGTGGCGTT 
CTGCACTTGA GTGACTTGGA TAATGATGGC TTAGATGAAA TCATCGTAGC AGCCCCGCTG 
AGGATCACAG ACGCAACTGC GGGACTGATG GGGGAAGAGG ATGGCCGTGT TTATGTGTTT 
AATGGCAAAC AGATCACCGT GGGTGACGTG ACAGGCAAAT GCAAATCATG GGTAACTCCG 
TGTCCAGAAG AAAAGGCCCA ATATGTACTA ATTTCTCCTG AAGCAGGCTC AAGGTTTGGG 
AGCTCTGTGA TCACTGTGAG GTCGAAGGAA AAGAATCAAG TCATCATTGC TGCTGGAAGG 
AGTTCTCTGG GAGCCCGACT CTCTGGGGTG CTTCATATCT ATAGGCTCGG CCAAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777241.1
CDS	40..2559
Translation	MSAFRFWSGLLMLLGFLCPRSSPCGISTHIEIGHRALEFLHLQDGSINYKELLLRHQDAYQAGSVFPDSFYPSICERGQFHDVSESTHWTPFLNASVHYIRKNYPLPWDEDTEKLVAFLFGITSHMVADVNWHSLGIEQGFLRTMAAIDFHNSYPEAHPAGDFGGDVLSQFEFKFNYLSRHWYVPAEDLLGIYRELYGRIVITKKAIVDCSYLQFLEMYAEMLAISKLYPTYSVKSPFLVEQFQEYFLGGLEDMAFWSTNIYHLTSYMLKNGTSNCNLPENPLFITCGGQQNNTHGSKVQKNGFHKNVTAALTKNIGKHINYTKRGVFFSVDSWTMDSLSFMYKSLERSIREMFIGSSQPLTHVSSPAASYYLSFPYTRLGWAMTSADLNQDGYGDLVVGAPGYSHPGRIHVGRVYLIYGNDLGLPRIDLDLDKEAHGILEGFQPSGRFGSAVAVLDFNVDGVPDLAVGAPSVGSEKLTYTGAVYVYFGSKQGQLSSSPNVTISCQDTYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGSSYSSREKLNVEAANWMVKGEEDFAWLGYSLHGVNVNNRTLLLAGSPTWKDTSSQGHLFRTRDEKQSPGRVYGYFPPICQSWFTISGDKAMGKLGTSLSSGHVMVNGTRTQVLLVGAPTQDVVSKVSFLTMTLHQGGSTRMYELTPDSQPSLLSTFSGNRRFSRFGGVLHLSDLDNDGLDEIIVAAPLRITDATAGLMGEEDGRVYVFNGKQITVGDVTGKCKSWVTPCPEEKAQYVLISPEAGSRFGSSVITVRSKEKNQVIIAAGRSSLGARLSGVLHIYRLGQD

Target ORF information:

RefSeq Version	NM_174816.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174816.2

ORF Insert Sequence:

ATGTCTGCTT TCAGATTCTG GTCAGGACTG CTGATGCTAC TGGGCTTCCT CTGCCCTAGA 
AGTTCACCAT GTGGCATTTC GACACACATA GAAATAGGAC ACAGAGCTCT GGAGTTTCTC 
CACCTTCAGG ATGGGAGTAT TAACTACAAA GAGCTGTTAC TTAGGCACCA GGATGCATAT 
CAGGCTGGAT CCGTGTTTCC TGACTCATTT TACCCTAGCA TCTGTGAGAG AGGACAATTC 
CATGACGTGT CAGAGAGCAC TCACTGGACT CCATTTCTTA ACGCAAGTGT TCATTATATC 
CGGAAGAACT ATCCTCTTCC CTGGGATGAG GACACAGAGA AATTGGTAGC TTTCTTGTTT 
GGAATTACGT CTCACATGGT GGCTGATGTC AACTGGCATA GCCTGGGTAT TGAACAAGGA 
TTCCTTAGGA CGATGGCTGC CATTGATTTT CACAACTCCT ATCCCGAGGC ACATCCGGCT 
GGTGATTTCG GAGGAGACGT GTTGAGCCAG TTCGAGTTTA AATTTAATTA CCTCTCACGG 
CACTGGTATG TGCCTGCTGA AGATCTCCTG GGAATTTATA GAGAACTCTA CGGCCGAATA 
GTCATCACCA AAAAAGCCAT TGTTGACTGT TCATACCTTC AATTCTTGGA AATGTATGCG 
GAGATGTTAG CTATTTCCAA GCTTTATCCC ACTTATTCTG TAAAATCCCC ATTTTTGGTG 
GAACAATTTC AAGAATACTT CCTAGGAGGG CTGGAAGATA TGGCGTTTTG GTCCACTAAT 
ATTTACCATC TGACAAGTTA CATGTTAAAG AACGGGACCA GTAACTGCAA CCTCCCTGAG 
AACCCTCTGT TCATCACATG TGGCGGTCAA CAAAACAACA CCCATGGCTC AAAAGTACAG 
AAAAATGGTT TTCATAAAAA TGTGACTGCA GCCCTAACTA AAAATATTGG AAAGCATATA 
AACTATACCA AAAGAGGAGT GTTCTTTAGT GTGGATTCCT GGACCATGGA TTCCTTATCC 
TTCATGTACA AGTCTTTGGA GAGGAGTATA CGGGAGATGT TTATTGGCAG CTCTCAGCCA 
CTGACACATG TTTCTAGCCC CGCAGCATCT TACTACTTGT CATTTCCCTA CACAAGGCTT 
GGTTGGGCAA TGACTTCAGC TGATCTCAAC CAGGATGGAT ACGGTGACCT GGTGGTGGGT 
GCCCCTGGCT ACAGCCACCC AGGCCGCATT CACGTGGGGC GCGTGTACCT CATCTATGGC 
AATGACCTGG GCTTGCCCCG TATCGACCTG GACCTGGACA AGGAGGCCCA CGGGATCCTG 
GAGGGTTTCC AGCCCTCAGG TCGATTTGGC TCGGCTGTGG CTGTGCTAGA CTTTAACGTG 
GATGGCGTGC CTGACCTGGC GGTGGGAGCC CCCTCGGTGG GCTCCGAGAA GCTCACATAC 
ACAGGTGCAG TGTATGTCTA CTTCGGTTCC AAACAAGGAC AACTATCTTC TTCCCCCAAC 
GTCACCATCT CTTGCCAGGA TACCTACTGT AACTTGGGCT GGACCCTCCT GGCGGCAGAT 
GTGAATGGAG ATAGTGAACC GGACCTGGTG ATTGGCTCCC CTTTTGCTCC AGGTGGAGGG 
AAACAGAAGG GAATTGTGGC TGCATTTTAC TCTGGCTCCA GTTACAGCAG CCGAGAAAAG 
CTGAATGTGG AGGCTGCCAA CTGGATGGTG AAAGGCGAGG AGGACTTTGC TTGGTTGGGG 
TACTCCCTTC ACGGTGTCAA TGTCAACAAC AGGACTTTGC TCCTGGCTGG AAGCCCGACC 
TGGAAGGACA CCAGTAGTCA GGGCCACTTG TTCCGCACTC GTGATGAGAA ACAGAGCCCT 
GGACGGGTGT ACGGCTATTT CCCGCCAATC TGTCAAAGCT GGTTTACCAT TTCCGGAGAC 
AAGGCAATGG GGAAACTGGG TACCTCCCTG TCTAGTGGCC ACGTGATGGT GAACGGGACC 
CGGACCCAAG TGCTGCTGGT GGGGGCCCCG ACTCAAGATG TCGTGTCTAA GGTATCATTC 
CTGACCATGA CCCTGCACCA AGGTGGGAGC ACTCGGATGT ATGAACTGAC ACCTGACTCA 
CAGCCTTCTC TGCTCAGCAC CTTCAGTGGA AACCGCCGCT TCTCCCGATT TGGTGGCGTT 
CTGCACTTGA GTGACTTGGA TAATGATGGC TTAGATGAAA TCATCGTAGC AGCCCCGCTG 
AGGATCACAG ACGCAACTGC GGGACTGATG GGGGAAGAGG ATGGCCGTGT TTATGTGTTT 
AATGGCAAAC AGATCACCGT GGGTGACGTG ACAGGCAAAT GCAAATCATG GGTAACTCCG 
TGTCCAGAAG AAAAGGCCCA ATATGTACTA ATTTCTCCTG AAGCAGGCTC AAGGTTTGGG 
AGCTCTGTGA TCACTGTGAG GTCGAAGGAA AAGAATCAAG TCATCATTGC TGCTGGAAGG 
AGTTCTCTGG GAGCCCGACT CTCTGGGGTG CTTCATATCT ATAGGCTCGG CCAAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_015459945.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315431.1 Bos taurus glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32230
Clone ID Related Accession (Same CDS sequence)	XM_015459945.1
Accession Version	XM_015459945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAGA AATCGACTCT TGTGAGATTC TGGTCAGGAC TGCTGATGCT ACTGGGCTTC 
CTCTGCCCTA GAAGTTCACC ATGTGGCATT TCGACACACA TAGAAATAGG ACACAGAGCT 
CTGGAGTTTC TCCACCTTCA GGATGGGAGT ATTAACTACA AAGAGCTGTT ACTTAGGCAC 
CAGGATGCAT ATCAGGCTGG ATCCGTGTTT CCTGACTCAT TTTACCCTAG CATCTGTGAG 
AGAGGACAAT TCCATGACGT GTCAGAGAGC ACTCACTGGA CTCCATTTCT TAACGCAAGT 
GTTCATTATA TCCGGAAGAA CTATCCTCTT CCCTGGGATG AGGACACAGA GAAACTGGTA 
GCTTTCTTGT TTGGAATTAC GTCTCACATG GTGGCTGATG TCAACTGGCA TAGCCTGGGT 
ATTGAACAAG GATTCCTTAG GACGATGGCT GCCATTGATT TTCACAACTC CTATCCCGAG 
GCACATCCGG CTGGTGATTT CGGAGGAGAC GTGTTGAGCC AGTTCGAGTT TAAATTTAAT 
TACCTCTCAC GGCACTGGTA TGTGCCTGCT GAAGATCTCC TGGGAATTTA TAAAGAACTC 
TATGGCCGAG TAGTCATCAC CAAAAAAGCC ATTGTTGACT GTTCATACCT TCAGTTCTTG 
GAAATGTATG CGGAGATGTT AGCTATTTCC AAGCTTTATC CCACTTATTC TGTAAAATCC 
CCATTTTTGG TGGAACAATT TCAAGAATAC TTCCTAGGAG GGCTGGAAGA TATGGCGTTT 
TGGTCCACTA ATATTTACCA TCTGACAAGT TACATGTTAA AGAACGGGAC CAGTAACTGC 
AACCTCCCTG AGAACCCTCT GTTCATCACA TGTGGCGGTC AACAAAACAA CACCCATGGC 
TCAAAAGTAC AGAAAAATGG TTTTCATAAA AATGTGACTG CAGCCCTAAC TAAAAATATT 
GGAAAGCATA TAAACTATAC CAAAAGAGGA GTGTTCTTTA GTGTGGATTC CTGGACCATG 
GATTCCTTAT CCTTCATGTA CAAGTCTTTG GAGAGGAGTA TACGGGAGAT GTTTATTGGC 
AGCTCTCAGC CACTGACACA TGTTTCTAGC CCCTCAGCAT CTTACTACTT GTCATTTCCC 
TACACAAGGC TTGGTTGGGC AATGACTTCA GCTGATCTCA ACCAGGATGG ATACGGTGAC 
CTGGTGGTGG GTGCCCCTGG CTACAGCCAC CCAGGCCGCA TTCACGTGGG GCGCGTGTAC 
CTCATCTATG GCAATGACCT GGGCTTGCCC CCTGTCGACC TGGACCTGGA CAAGGAGGCC 
CACGAGATCC TGGAGGGTTT CCAGCCCTCA GGTCGATTTG GCTCGGCTGT GGCTGTGCTA 
GACTTTAACG TGGATGGCGT GCCTGACCTG GCCGTGGGAG CCCCCTCGGT GGGCTCCGAG 
AAGCTCACAT ACACAGGTGC AGTGTATGTC TACTTCGGTT CCAAACAAGG ACAACTATCT 
TCTTCCCCCA ACGTCACCAT CTCTTGCCAG GATACCTACT GTAACTTGGG CTGGACCCTC 
CTGGCGGCAG ATGTGAATGG AGATAGTGAA CCGGACCTGG TGATTGGCTC CCCTTTTGCT 
CCAGGTGGAG GGAAACAGAA GGGAATTGTG GCTGCATTTT ACTCTGGCTC CAGTTACAGC 
AGCCAAGAAA AGCTGAATGT GGAGGCTGCC AACTGGATGG TGAAAGGCGA GGAGGACTTT 
GCTTGGTTGG GGTACTCCCT TCACGGTGTC AATGTCAACA ACAGGACTTT GCTCCTGGCT 
GGAAGCCCGA CCTGGAAGGA CACCAGTAGT CAGGGCCACT TGTTCCGCAC TCGTGATGAG 
AAACAGAGCC CTGGACGGGT GTACGGCTAT TTCCCGCCAA TCTGTCAAAG CTGGTTTACC 
ATTTCCGGAG ACAAGGCAAT GGGGAAACTG GGTACCTCCC TGTCTAGTGG CCACGTGATG 
GTGAACGGGA CCCGGACCCA AGTGCTGCTG GTGGGGGCCC CGACTCAAGA TGTCGTGTCT 
AAGGTATCAT TCCTGACCAT GACCCTGCAC CAAGGTGGGA GCACTCGGAT GTATGAACTG 
ACACCTGACT CACAGCCTTC TCTGCTCAGC ACCTTCAGTG GAAACCGCCG CTTCTCCCGA 
TTTGGTGGCG TTCTGCACTT GAGTGACTTG GATAATGATG GCTCAGATGA AATCATCGTA 
GCAGCCCCGC TGAGGATCAC AGACGCAACT GCGGGACTGA TGGGGGAAGA GGATGGCCGT 
GTTTATGTGT TTAATGGCAA ACAGATCACC GTGGGTGACG TGACAGGCAA ATGCAAATCA 
TGGGTAACTC CGTGTCCAGA AGAAAAGGCC CAATATGTAC TAATTTCTCC TGAAGCAGGC 
TCAAGGTTTG GGAGCTCTGT GATCACTGTG AGGTCGAAGG AAAAGAATCA AGTCATCATT 
GCTGCTGGAA GGAGTTCTCT GGGAGCCCGA CTCTCTGGGG TGCTTCATAT CTATAGGCTC 
GGCCAAGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315431.1
CDS	984..3515
Translation	MQEKSTLVRFWSGLLMLLGFLCPRSSPCGISTHIEIGHRALEFLHLQDGSINYKELLLRHQDAYQAGSVFPDSFYPSICERGQFHDVSESTHWTPFLNASVHYIRKNYPLPWDEDTEKLVAFLFGITSHMVADVNWHSLGIEQGFLRTMAAIDFHNSYPEAHPAGDFGGDVLSQFEFKFNYLSRHWYVPAEDLLGIYKELYGRVVITKKAIVDCSYLQFLEMYAEMLAISKLYPTYSVKSPFLVEQFQEYFLGGLEDMAFWSTNIYHLTSYMLKNGTSNCNLPENPLFITCGGQQNNTHGSKVQKNGFHKNVTAALTKNIGKHINYTKRGVFFSVDSWTMDSLSFMYKSLERSIREMFIGSSQPLTHVSSPSASYYLSFPYTRLGWAMTSADLNQDGYGDLVVGAPGYSHPGRIHVGRVYLIYGNDLGLPPVDLDLDKEAHEILEGFQPSGRFGSAVAVLDFNVDGVPDLAVGAPSVGSEKLTYTGAVYVYFGSKQGQLSSSPNVTISCQDTYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGSSYSSQEKLNVEAANWMVKGEEDFAWLGYSLHGVNVNNRTLLLAGSPTWKDTSSQGHLFRTRDEKQSPGRVYGYFPPICQSWFTISGDKAMGKLGTSLSSGHVMVNGTRTQVLLVGAPTQDVVSKVSFLTMTLHQGGSTRMYELTPDSQPSLLSTFSGNRRFSRFGGVLHLSDLDNDGSDEIIVAAPLRITDATAGLMGEEDGRVYVFNGKQITVGDVTGKCKSWVTPCPEEKAQYVLISPEAGSRFGSSVITVRSKEKNQVIIAAGRSSLGARLSGVLHIYRLGQD

Target ORF information:

RefSeq Version	XM_015459945.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459945.1

ORF Insert Sequence:

ATGCAAGAGA AATCGACTCT TGTGAGATTC TGGTCAGGAC TGCTGATGCT ACTGGGCTTC 
CTCTGCCCTA GAAGTTCACC ATGTGGCATT TCGACACACA TAGAAATAGG ACACAGAGCT 
CTGGAGTTTC TCCACCTTCA GGATGGGAGT ATTAACTACA AAGAGCTGTT ACTTAGGCAC 
CAGGATGCAT ATCAGGCTGG ATCCGTGTTT CCTGACTCAT TTTACCCTAG CATCTGTGAG 
AGAGGACAAT TCCATGACGT GTCAGAGAGC ACTCACTGGA CTCCATTTCT TAACGCAAGT 
GTTCATTATA TCCGGAAGAA CTATCCTCTT CCCTGGGATG AGGACACAGA GAAACTGGTA 
GCTTTCTTGT TTGGAATTAC GTCTCACATG GTGGCTGATG TCAACTGGCA TAGCCTGGGT 
ATTGAACAAG GATTCCTTAG GACGATGGCT GCCATTGATT TTCACAACTC CTATCCCGAG 
GCACATCCGG CTGGTGATTT CGGAGGAGAC GTGTTGAGCC AGTTCGAGTT TAAATTTAAT 
TACCTCTCAC GGCACTGGTA TGTGCCTGCT GAAGATCTCC TGGGAATTTA TAAAGAACTC 
TATGGCCGAG TAGTCATCAC CAAAAAAGCC ATTGTTGACT GTTCATACCT TCAGTTCTTG 
GAAATGTATG CGGAGATGTT AGCTATTTCC AAGCTTTATC CCACTTATTC TGTAAAATCC 
CCATTTTTGG TGGAACAATT TCAAGAATAC TTCCTAGGAG GGCTGGAAGA TATGGCGTTT 
TGGTCCACTA ATATTTACCA TCTGACAAGT TACATGTTAA AGAACGGGAC CAGTAACTGC 
AACCTCCCTG AGAACCCTCT GTTCATCACA TGTGGCGGTC AACAAAACAA CACCCATGGC 
TCAAAAGTAC AGAAAAATGG TTTTCATAAA AATGTGACTG CAGCCCTAAC TAAAAATATT 
GGAAAGCATA TAAACTATAC CAAAAGAGGA GTGTTCTTTA GTGTGGATTC CTGGACCATG 
GATTCCTTAT CCTTCATGTA CAAGTCTTTG GAGAGGAGTA TACGGGAGAT GTTTATTGGC 
AGCTCTCAGC CACTGACACA TGTTTCTAGC CCCTCAGCAT CTTACTACTT GTCATTTCCC 
TACACAAGGC TTGGTTGGGC AATGACTTCA GCTGATCTCA ACCAGGATGG ATACGGTGAC 
CTGGTGGTGG GTGCCCCTGG CTACAGCCAC CCAGGCCGCA TTCACGTGGG GCGCGTGTAC 
CTCATCTATG GCAATGACCT GGGCTTGCCC CCTGTCGACC TGGACCTGGA CAAGGAGGCC 
CACGAGATCC TGGAGGGTTT CCAGCCCTCA GGTCGATTTG GCTCGGCTGT GGCTGTGCTA 
GACTTTAACG TGGATGGCGT GCCTGACCTG GCCGTGGGAG CCCCCTCGGT GGGCTCCGAG 
AAGCTCACAT ACACAGGTGC AGTGTATGTC TACTTCGGTT CCAAACAAGG ACAACTATCT 
TCTTCCCCCA ACGTCACCAT CTCTTGCCAG GATACCTACT GTAACTTGGG CTGGACCCTC 
CTGGCGGCAG ATGTGAATGG AGATAGTGAA CCGGACCTGG TGATTGGCTC CCCTTTTGCT 
CCAGGTGGAG GGAAACAGAA GGGAATTGTG GCTGCATTTT ACTCTGGCTC CAGTTACAGC 
AGCCAAGAAA AGCTGAATGT GGAGGCTGCC AACTGGATGG TGAAAGGCGA GGAGGACTTT 
GCTTGGTTGG GGTACTCCCT TCACGGTGTC AATGTCAACA ACAGGACTTT GCTCCTGGCT 
GGAAGCCCGA CCTGGAAGGA CACCAGTAGT CAGGGCCACT TGTTCCGCAC TCGTGATGAG 
AAACAGAGCC CTGGACGGGT GTACGGCTAT TTCCCGCCAA TCTGTCAAAG CTGGTTTACC 
ATTTCCGGAG ACAAGGCAAT GGGGAAACTG GGTACCTCCC TGTCTAGTGG CCACGTGATG 
GTGAACGGGA CCCGGACCCA AGTGCTGCTG GTGGGGGCCC CGACTCAAGA TGTCGTGTCT 
AAGGTATCAT TCCTGACCAT GACCCTGCAC CAAGGTGGGA GCACTCGGAT GTATGAACTG 
ACACCTGACT CACAGCCTTC TCTGCTCAGC ACCTTCAGTG GAAACCGCCG CTTCTCCCGA 
TTTGGTGGCG TTCTGCACTT GAGTGACTTG GATAATGATG GCTCAGATGA AATCATCGTA 
GCAGCCCCGC TGAGGATCAC AGACGCAACT GCGGGACTGA TGGGGGAAGA GGATGGCCGT 
GTTTATGTGT TTAATGGCAA ACAGATCACC GTGGGTGACG TGACAGGCAA ATGCAAATCA 
TGGGTAACTC CGTGTCCAGA AGAAAAGGCC CAATATGTAC TAATTTCTCC TGAAGCAGGC 
TCAAGGTTTG GGAGCTCTGT GATCACTGTG AGGTCGAAGG AAAAGAATCA AGTCATCATT 
GCTGCTGGAA GGAGTTCTCT GGGAGCCCGA CTCTCTGGGG TGCTTCATAT CTATAGGCTC 
GGCCAAGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Phospholipase cleavage of D- and L-chiro-glycosylphosphoinositides asymmetrically incorporated into liposomal membranes.
Chemistry (Weinheim an der Bergstrasse, Germany)12(5)1513-28(2006 Feb)
Bonilla JB,Cid MB,Contreras FX,Go?i FM,Mart?n-Lomas M

Phosphorylation of glycosyl-phosphatidylinositol by phosphatidylinositol 3-kinase changes its properties as a substrate for phospholipases.
FEBS letters579(1)59-65(2005 Jan)
Jones DR,Pa?eda C,Villar AV,Alonso A,Go?i FM,B?tikofer P,Brodbeck U,Shepherd PR,Varela-Nieto I

Hyperoxia alters phorbol ester-induced phospholipase D activation in bovine lung microvascular endothelial cells.
Antioxidants & redox signaling5(2)217-28(2003 Apr)
Roy S,Parinandi N,Zeigelstein R,Hu Q,Pei Y,Travers JB,Natarajan V

Purification and characterization of glycosyl-phosphatidylinositol-specific phospholipase D.
The Journal of biological chemistry265(29)17738-45(1990 Oct)
Huang KS,Li S,Fung WJ,Hulmes JD,Reik L,Pan YC,Low MG

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GPLD1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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