TNNT2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNNT2
Entrez Gene ID	286816
Full Name	troponin T2, cardiac type
General protein information	Preferred Names troponin T2, cardiac type Names troponin T, cardiac muscle cTnT cardiac muscle troponin T tnTc
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024976056.1	XP_024831824.1	troponin T, cardiac muscle isoform X3
NM_174771.3	NP_777196.1	troponin T, cardiac muscle
XM_024976054.1	XP_024831822.1	troponin T, cardiac muscle isoform X1
XM_024976057.1	XP_024831825.1	troponin T, cardiac muscle isoform X4
XM_024976060.1	XP_024831828.1	troponin T, cardiac muscle isoform X6
XM_024976055.1	XP_024831823.1	troponin T, cardiac muscle isoform X2
XM_024976059.1	XP_024831827.1	troponin T, cardiac muscle isoform X5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TNNT2	XP_001145598.1
	TNNT2	XP_002802032.1
Mus musculus (house mouse)	Tnnt2	NP_001123650.1
Gallus gallus (chicken)	TNNT2	NP_990780.1
Xenopus tropicalis (tropical clawed frog)	LOC100493699	XP_004919786.1
Canis lupus familiaris (dog)	TNNT2	NP_001003012.1
Bos taurus (cattle)	TNNT2	NP_777196.1
Homo sapiens (human)	TNNT2	NP_001001432.1
Rattus norvegicus (Norway rat)	Tnnt2	NP_036808.1
Danio rerio (zebrafish)	tnnt2a	NP_690853.1

Gene Ontology
Bibliography

Related articles in PubMed

FRET study of the structural and kinetic effects of PKC phosphomimetic cardiac troponin T mutants on thin filament regulation.
Schlecht W, Zhou Z, Li KL, Rieck D, Ouyang Y, Dong WJ
Archives of biochemistry and biophysics550-5511-11(2014 May)

Unexpected cross-species contamination in genome sequencing projects.
Merchant S, Wood DE, Salzberg SL
PeerJ2e675(2014)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage.
Zhang Z, Biesiadecki BJ, Jin JP
Biochemistry45(38)11681-94(2006 Sep)

Functional consequences of troponin T mutations found in hypertrophic cardiomyopathy.
Tobacman LS, Lin D, Butters C, Landis C, Back N, Pavlov D, Homsher E
The Journal of biological chemistry274(40)28363-70(1999 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Significant changes in thin filament Ca2+-sensitivity, structure and kinetics are brought about through PKC phosphorylation of cardiac troponin T.
Title: FRET study of the structural and kinetic effects of PKC phosphomimetic cardiac troponin T mutants on thin filament regulation.

The mu-calpain-mediated proteolytic modification of TnT by removing the NH2-terminal variable region of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions.
Title: Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage.

The following TNNT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43751	NM_174771.3 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa223438	XM_024976054.1 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa223439	XM_024976055.1 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa223440	XM_024976056.1 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa223441	XM_024976057.1 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa223442	XM_024976059.1 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa223443	XM_024976060.1 Latest version!	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNNT2 ( NM_174771.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777196.1 Bos taurus troponin T2, cardiac type (TNNT2), mRNA.

Price & Availability↑

CloneID	OBa43751
Clone ID Related Accession (Same CDS sequence)	NM_174771.3
Accession Version	NM_174771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134435.1. On Apr 1, 2007 this sequence version replaced NM_174771.2. ##Evidence-Data-START## CDS exon combination :: BC134435.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145418, SAMN03145438 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGATG GAGAAGAAGA GGAAGGTAGA GAGGCTGAAG ATGGCCCGGT GGAGGAGTTC 
AAGCCCAAGC CCAGGCCCTT CATGCCCAAC TTGGTGCCGC CCAAGATCCC TGATGGAGAG 
AGGGTGGACT TCGATGACAT ACACCGGAAG CGCATGGAGA AGGACCTCAA CGAGCTGCAG 
ACACTGATCG AGGCGCATTT CGAGAACCGC AAGAAGGAGG AGGAGGAGCT GGTCTCCCTC 
AAAGACAGGA TCGAGAAGCG GCGGGCAGAA CGCGCGGAGC AGCAGCGCAT CCGCGCGGAG 
CGCGAGAAGG AGCGGCAGAC GCGCCTGGCG GAGGAGCGAG CCCGCCGAGA GGAGGAGGAG 
AGCCGCAGGA AGGCGGAGGA CGAGGCGCGC AAGAAGAAGG CTCTGTCCAA CATGATGCAC 
TTCGGCGGCT ACATCCAGAA GGCCCAGACA GAGCGTAAAA GTGGGAAGAG ACAGACCGAG 
CGGGAGAAGA AGAAGAAGAT TCTGGCCGAG CGGAGGAAGG TGCTGGCCAT CGACCACCTG 
AACGAAGACC AGCTGAGGGA GAAGGCCAAG GAGCTGTGGC AGAACATCTA CGACCTGGAG 
GCGGAGAAGT TCGACCTGCA AGAGAAGTTC AAGCAGCAGA AATACGAGAT CAATGTTCTC 
CGAAACAGGA TCAATGACAA CCAGAAAGTC TCCAAGACTC GAGGGAAGGC CAAGGTCACG 
GGGCGCTGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777196.1
CDS	72..926
Translation	MSDVEEAVEEYEEQEEAAEEEHEEAVEEEAGGEAEAGEPCTADGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	NM_174771.3
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174771.3

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGATG GAGAAGAAGA GGAAGGTAGA GAGGCTGAAG ATGGCCCGGT GGAGGAGTTC 
AAGCCCAAGC CCAGGCCCTT CATGCCCAAC TTGGTGCCGC CCAAGATCCC TGATGGAGAG 
AGGGTGGACT TCGATGACAT ACACCGGAAG CGCATGGAGA AGGACCTCAA CGAGCTGCAG 
ACACTGATCG AGGCGCATTT CGAGAACCGC AAGAAGGAGG AGGAGGAGCT GGTCTCCCTC 
AAAGACAGGA TCGAGAAGCG GCGGGCAGAA CGCGCGGAGC AGCAGCGCAT CCGCGCGGAG 
CGCGAGAAGG AGCGGCAGAC GCGCCTGGCG GAGGAGCGAG CCCGCCGAGA GGAGGAGGAG 
AGCCGCAGGA AGGCGGAGGA CGAGGCGCGC AAGAAGAAGG CTCTGTCCAA CATGATGCAC 
TTCGGCGGCT ACATCCAGAA GGCCCAGACA GAGCGTAAAA GTGGGAAGAG ACAGACCGAG 
CGGGAGAAGA AGAAGAAGAT TCTGGCCGAG CGGAGGAAGG TGCTGGCCAT CGACCACCTG 
AACGAAGACC AGCTGAGGGA GAAGGCCAAG GAGCTGTGGC AGAACATCTA CGACCTGGAG 
GCGGAGAAGT TCGACCTGCA AGAGAAGTTC AAGCAGCAGA AATACGAGAT CAATGTTCTC 
CGAAACAGGA TCAATGACAA CCAGAAAGTC TCCAAGACTC GAGGGAAGGC CAAGGTCACG 
GGGCGCTGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNT2 ( XM_024976054.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831822.1 Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223438
Clone ID Related Accession (Same CDS sequence)	XM_024976054.1
Accession Version	XM_024976054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGAAG ATGGAGAAGA AGAGGAAGGT AGAGAGGCTG AAGATGGCCC GGTGGAGGAG 
TTCAAGCCCA AGCCCAGGCC CTTCATGCCC AACTTGGTGC CGCCCAAGAT CCCTGATGGA 
GAGAGGGTGG ACTTCGATGA CATACACCGG AAGCGCATGG AGAAGGACCT CAACGAGCTG 
CAGACACTGA TCGAGGCGCA TTTCGAGAAC CGCAAGAAGG AGGAGGAGGA GCTGGTCTCC 
CTCAAAGACA GGATCGAGAA GCGGCGGGCA GAACGCGCGG AGCAGCAGCG CATCCGCGCG 
GAGCGCGAGA AGGAGCGGCA GACGCGCCTG GCGGAGGAGC GAGCCCGCCG AGAGGAGGAG 
GAGAGCCGCA GGAAGGCGGA GGACGAGGCG CGCAAGAAGA AGGCTCTGTC CAACATGATG 
CACTTCGGCG GCTACATCCA GAAGGCCCAG ACAGAGCGTA AAAGTGGGAA GAGACAGACC 
GAGCGGGAGA AGAAGAAGAA GATTCTGGCC GAGCGGAGGA AGGTGCTGGC CATCGACCAC 
CTGAACGAAG ACCAGCTGAG GGAGAAGGCC AAGGAGCTGT GGCAGAACAT CTACGACCTG 
GAGGCGGAGA AGTTCGACCT GCAAGAGAAG TTCAAGCAGC AGAAATACGA GATCAATGTT 
CTCCGAAACA GGATCAATGA CAACCAGAAA GTCTCCAAGA CTCGAGGGAA GGCCAAGGTC 
ACGGGGCGCT GGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831822.1
CDS	122..979
Translation	MSDVEEAVEEYEEQEEAAEEEHEEAVEEEAGGEAEAGEPCTAEDGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	XM_024976054.1
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976054.1

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGAAG ATGGAGAAGA AGAGGAAGGT AGAGAGGCTG AAGATGGCCC GGTGGAGGAG 
TTCAAGCCCA AGCCCAGGCC CTTCATGCCC AACTTGGTGC CGCCCAAGAT CCCTGATGGA 
GAGAGGGTGG ACTTCGATGA CATACACCGG AAGCGCATGG AGAAGGACCT CAACGAGCTG 
CAGACACTGA TCGAGGCGCA TTTCGAGAAC CGCAAGAAGG AGGAGGAGGA GCTGGTCTCC 
CTCAAAGACA GGATCGAGAA GCGGCGGGCA GAACGCGCGG AGCAGCAGCG CATCCGCGCG 
GAGCGCGAGA AGGAGCGGCA GACGCGCCTG GCGGAGGAGC GAGCCCGCCG AGAGGAGGAG 
GAGAGCCGCA GGAAGGCGGA GGACGAGGCG CGCAAGAAGA AGGCTCTGTC CAACATGATG 
CACTTCGGCG GCTACATCCA GAAGGCCCAG ACAGAGCGTA AAAGTGGGAA GAGACAGACC 
GAGCGGGAGA AGAAGAAGAA GATTCTGGCC GAGCGGAGGA AGGTGCTGGC CATCGACCAC 
CTGAACGAAG ACCAGCTGAG GGAGAAGGCC AAGGAGCTGT GGCAGAACAT CTACGACCTG 
GAGGCGGAGA AGTTCGACCT GCAAGAGAAG TTCAAGCAGC AGAAATACGA GATCAATGTT 
CTCCGAAACA GGATCAATGA CAACCAGAAA GTCTCCAAGA CTCGAGGGAA GGCCAAGGTC 
ACGGGGCGCT GGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNT2 ( XM_024976055.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831823.1 Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa223439
Clone ID Related Accession (Same CDS sequence)	XM_024976055.1
Accession Version	XM_024976055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGAAG ATGGAGAAGA AGAGGAAGGT AGAGAGGCTG AAGATGGCCC GGTGGAGGAG 
TTCAAGCCCA AGCCCAGGCC CTTCATGCCC AACTTGGTGC CGCCCAAGAT CCCTGATGGA 
GAGAGGGTGG ACTTCGATGA CATACACCGG AAGCGCATGG AGAAGGACCT CAACGAGCTG 
CAGACACTGA TCGAGGCGCA TTTCGAGAAC CGCAAGAAGG AGGAGGAGGA GCTGGTCTCC 
CTCAAAGACA GGATCGAGAA GCGGCGGGCA GAACGCGCGG AGCAGCAGCG CATCCGCGCG 
GAGCGCGAGA AGGAGCGGCA GACGCGCCTG GCGGAGGAGC GAGCCCGCCG AGAGGAGGAG 
GAGAGCCGCA GGAAGGCGGA GGACGAGGCG CGCAAGAAGA AGGCTCTGTC CAACATGATG 
CACTTCGGCG GCTACATCCA GAAGACAGAG CGTAAAAGTG GGAAGAGACA GACCGAGCGG 
GAGAAGAAGA AGAAGATTCT GGCCGAGCGG AGGAAGGTGC TGGCCATCGA CCACCTGAAC 
GAAGACCAGC TGAGGGAGAA GGCCAAGGAG CTGTGGCAGA ACATCTACGA CCTGGAGGCG 
GAGAAGTTCG ACCTGCAAGA GAAGTTCAAG CAGCAGAAAT ACGAGATCAA TGTTCTCCGA 
AACAGGATCA ATGACAACCA GAAAGTCTCC AAGACTCGAG GGAAGGCCAA GGTCACGGGG 
CGCTGGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831823.1
CDS	122..973
Translation	MSDVEEAVEEYEEQEEAAEEEHEEAVEEEAGGEAEAGEPCTAEDGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	XM_024976055.1
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976055.1

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGAAG ATGGAGAAGA AGAGGAAGGT AGAGAGGCTG AAGATGGCCC GGTGGAGGAG 
TTCAAGCCCA AGCCCAGGCC CTTCATGCCC AACTTGGTGC CGCCCAAGAT CCCTGATGGA 
GAGAGGGTGG ACTTCGATGA CATACACCGG AAGCGCATGG AGAAGGACCT CAACGAGCTG 
CAGACACTGA TCGAGGCGCA TTTCGAGAAC CGCAAGAAGG AGGAGGAGGA GCTGGTCTCC 
CTCAAAGACA GGATCGAGAA GCGGCGGGCA GAACGCGCGG AGCAGCAGCG CATCCGCGCG 
GAGCGCGAGA AGGAGCGGCA GACGCGCCTG GCGGAGGAGC GAGCCCGCCG AGAGGAGGAG 
GAGAGCCGCA GGAAGGCGGA GGACGAGGCG CGCAAGAAGA AGGCTCTGTC CAACATGATG 
CACTTCGGCG GCTACATCCA GAAGACAGAG CGTAAAAGTG GGAAGAGACA GACCGAGCGG 
GAGAAGAAGA AGAAGATTCT GGCCGAGCGG AGGAAGGTGC TGGCCATCGA CCACCTGAAC 
GAAGACCAGC TGAGGGAGAA GGCCAAGGAG CTGTGGCAGA ACATCTACGA CCTGGAGGCG 
GAGAAGTTCG ACCTGCAAGA GAAGTTCAAG CAGCAGAAAT ACGAGATCAA TGTTCTCCGA 
AACAGGATCA ATGACAACCA GAAAGTCTCC AAGACTCGAG GGAAGGCCAA GGTCACGGGG 
CGCTGGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNT2 ( XM_024976056.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831824.1 Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa223440
Clone ID Related Accession (Same CDS sequence)	XM_024976056.1
Accession Version	XM_024976056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGATG GAGAAGAAGA GGAAGGTAGA GAGGCTGAAG ATGGCCCGGT GGAGGAGTTC 
AAGCCCAAGC CCAGGCCCTT CATGCCCAAC TTGGTGCCGC CCAAGATCCC TGATGGAGAG 
AGGGTGGACT TCGATGACAT ACACCGGAAG CGCATGGAGA AGGACCTCAA CGAGCTGCAG 
ACACTGATCG AGGCGCATTT CGAGAACCGC AAGAAGGAGG AGGAGGAGCT GGTCTCCCTC 
AAAGACAGGA TCGAGAAGCG GCGGGCAGAA CGCGCGGAGC AGCAGCGCAT CCGCGCGGAG 
CGCGAGAAGG AGCGGCAGAC GCGCCTGGCG GAGGAGCGAG CCCGCCGAGA GGAGGAGGAG 
AGCCGCAGGA AGGCGGAGGA CGAGGCGCGC AAGAAGAAGG CTCTGTCCAA CATGATGCAC 
TTCGGCGGCT ACATCCAGAA GACAGAGCGT AAAAGTGGGA AGAGACAGAC CGAGCGGGAG 
AAGAAGAAGA AGATTCTGGC CGAGCGGAGG AAGGTGCTGG CCATCGACCA CCTGAACGAA 
GACCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAACA TCTACGACCT GGAGGCGGAG 
AAGTTCGACC TGCAAGAGAA GTTCAAGCAG CAGAAATACG AGATCAATGT TCTCCGAAAC 
AGGATCAATG ACAACCAGAA AGTCTCCAAG ACTCGAGGGA AGGCCAAGGT CACGGGGCGC 
TGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831824.1
CDS	105..953
Translation	MSDVEEAVEEYEEQEEAAEEEHEEAVEEEAGGEAEAGEPCTADGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	XM_024976056.1
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976056.1

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAAGC AGCCGAGGAA 
GAGCACGAGG AGGCGGTGGA AGAGGAGGCT GGAGGCGAGG CTGAGGCTGG GGAGCCCTGC 
ACCGCAGATG GAGAAGAAGA GGAAGGTAGA GAGGCTGAAG ATGGCCCGGT GGAGGAGTTC 
AAGCCCAAGC CCAGGCCCTT CATGCCCAAC TTGGTGCCGC CCAAGATCCC TGATGGAGAG 
AGGGTGGACT TCGATGACAT ACACCGGAAG CGCATGGAGA AGGACCTCAA CGAGCTGCAG 
ACACTGATCG AGGCGCATTT CGAGAACCGC AAGAAGGAGG AGGAGGAGCT GGTCTCCCTC 
AAAGACAGGA TCGAGAAGCG GCGGGCAGAA CGCGCGGAGC AGCAGCGCAT CCGCGCGGAG 
CGCGAGAAGG AGCGGCAGAC GCGCCTGGCG GAGGAGCGAG CCCGCCGAGA GGAGGAGGAG 
AGCCGCAGGA AGGCGGAGGA CGAGGCGCGC AAGAAGAAGG CTCTGTCCAA CATGATGCAC 
TTCGGCGGCT ACATCCAGAA GACAGAGCGT AAAAGTGGGA AGAGACAGAC CGAGCGGGAG 
AAGAAGAAGA AGATTCTGGC CGAGCGGAGG AAGGTGCTGG CCATCGACCA CCTGAACGAA 
GACCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAACA TCTACGACCT GGAGGCGGAG 
AAGTTCGACC TGCAAGAGAA GTTCAAGCAG CAGAAATACG AGATCAATGT TCTCCGAAAC 
AGGATCAATG ACAACCAGAA AGTCTCCAAG ACTCGAGGGA AGGCCAAGGT CACGGGGCGC 
TGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNT2 ( XM_024976057.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831825.1 Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X4, mRNA.

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CloneID	OBa223441
Clone ID Related Accession (Same CDS sequence)	XM_024976057.1
Accession Version	XM_024976057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAGCA CGAGGAGGCG 
GTGGAAGAGG AGGCTGGAGG CGAGGCTGAG GCTGGGGAGC CCTGCACCGC AGAAGATGGA 
GAAGAAGAGG AAGGTAGAGA GGCTGAAGAT GGCCCGGTGG AGGAGTTCAA GCCCAAGCCC 
AGGCCCTTCA TGCCCAACTT GGTGCCGCCC AAGATCCCTG ATGGAGAGAG GGTGGACTTC 
GATGACATAC ACCGGAAGCG CATGGAGAAG GACCTCAACG AGCTGCAGAC ACTGATCGAG 
GCGCATTTCG AGAACCGCAA GAAGGAGGAG GAGGAGCTGG TCTCCCTCAA AGACAGGATC 
GAGAAGCGGC GGGCAGAACG CGCGGAGCAG CAGCGCATCC GCGCGGAGCG CGAGAAGGAG 
CGGCAGACGC GCCTGGCGGA GGAGCGAGCC CGCCGAGAGG AGGAGGAGAG CCGCAGGAAG 
GCGGAGGACG AGGCGCGCAA GAAGAAGGCT CTGTCCAACA TGATGCACTT CGGCGGCTAC 
ATCCAGAAGG CCCAGACAGA GCGTAAAAGT GGGAAGAGAC AGACCGAGCG GGAGAAGAAG 
AAGAAGATTC TGGCCGAGCG GAGGAAGGTG CTGGCCATCG ACCACCTGAA CGAAGACCAG 
CTGAGGGAGA AGGCCAAGGA GCTGTGGCAG AACATCTACG ACCTGGAGGC GGAGAAGTTC 
GACCTGCAAG AGAAGTTCAA GCAGCAGAAA TACGAGATCA ATGTTCTCCG AAACAGGATC 
AATGACAACC AGAAAGTCTC CAAGACTCGA GGGAAGGCCA AGGTCACGGG GCGCTGGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831825.1
CDS	128..970
Translation	MSDVEEAVEEYEEQEEHEEAVEEEAGGEAEAGEPCTAEDGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	XM_024976057.1
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976057.1

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAGCA CGAGGAGGCG 
GTGGAAGAGG AGGCTGGAGG CGAGGCTGAG GCTGGGGAGC CCTGCACCGC AGAAGATGGA 
GAAGAAGAGG AAGGTAGAGA GGCTGAAGAT GGCCCGGTGG AGGAGTTCAA GCCCAAGCCC 
AGGCCCTTCA TGCCCAACTT GGTGCCGCCC AAGATCCCTG ATGGAGAGAG GGTGGACTTC 
GATGACATAC ACCGGAAGCG CATGGAGAAG GACCTCAACG AGCTGCAGAC ACTGATCGAG 
GCGCATTTCG AGAACCGCAA GAAGGAGGAG GAGGAGCTGG TCTCCCTCAA AGACAGGATC 
GAGAAGCGGC GGGCAGAACG CGCGGAGCAG CAGCGCATCC GCGCGGAGCG CGAGAAGGAG 
CGGCAGACGC GCCTGGCGGA GGAGCGAGCC CGCCGAGAGG AGGAGGAGAG CCGCAGGAAG 
GCGGAGGACG AGGCGCGCAA GAAGAAGGCT CTGTCCAACA TGATGCACTT CGGCGGCTAC 
ATCCAGAAGG CCCAGACAGA GCGTAAAAGT GGGAAGAGAC AGACCGAGCG GGAGAAGAAG 
AAGAAGATTC TGGCCGAGCG GAGGAAGGTG CTGGCCATCG ACCACCTGAA CGAAGACCAG 
CTGAGGGAGA AGGCCAAGGA GCTGTGGCAG AACATCTACG ACCTGGAGGC GGAGAAGTTC 
GACCTGCAAG AGAAGTTCAA GCAGCAGAAA TACGAGATCA ATGTTCTCCG AAACAGGATC 
AATGACAACC AGAAAGTCTC CAAGACTCGA GGGAAGGCCA AGGTCACGGG GCGCTGGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNT2 ( XM_024976059.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831827.1 Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X5, mRNA.

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CloneID	OBa223442
Clone ID Related Accession (Same CDS sequence)	XM_024976059.1
Accession Version	XM_024976059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAGCA CGAGGAGGCG 
GTGGAAGAGG AGGCTGGAGG CGAGGCTGAG GCTGGGGAGC CCTGCACCGC AGATGGAGAA 
GAAGAGGAAG GTAGAGAGGC TGAAGATGGC CCGGTGGAGG AGTTCAAGCC CAAGCCCAGG 
CCCTTCATGC CCAACTTGGT GCCGCCCAAG ATCCCTGATG GAGAGAGGGT GGACTTCGAT 
GACATACACC GGAAGCGCAT GGAGAAGGAC CTCAACGAGC TGCAGACACT GATCGAGGCG 
CATTTCGAGA ACCGCAAGAA GGAGGAGGAG GAGCTGGTCT CCCTCAAAGA CAGGATCGAG 
AAGCGGCGGG CAGAACGCGC GGAGCAGCAG CGCATCCGCG CGGAGCGCGA GAAGGAGCGG 
CAGACGCGCC TGGCGGAGGA GCGAGCCCGC CGAGAGGAGG AGGAGAGCCG CAGGAAGGCG 
GAGGACGAGG CGCGCAAGAA GAAGGCTCTG TCCAACATGA TGCACTTCGG CGGCTACATC 
CAGAAGGCCC AGACAGAGCG TAAAAGTGGG AAGAGACAGA CCGAGCGGGA GAAGAAGAAG 
AAGATTCTGG CCGAGCGGAG GAAGGTGCTG GCCATCGACC ACCTGAACGA AGACCAGCTG 
AGGGAGAAGG CCAAGGAGCT GTGGCAGAAC ATCTACGACC TGGAGGCGGA GAAGTTCGAC 
CTGCAAGAGA AGTTCAAGCA GCAGAAATAC GAGATCAATG TTCTCCGAAA CAGGATCAAT 
GACAACCAGA AAGTCTCCAA GACTCGAGGG AAGGCCAAGG TCACGGGGCG CTGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831827.1
CDS	105..944
Translation	MSDVEEAVEEYEEQEEHEEAVEEEAGGEAEAGEPCTADGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	XM_024976059.1
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976059.1

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAGCA CGAGGAGGCG 
GTGGAAGAGG AGGCTGGAGG CGAGGCTGAG GCTGGGGAGC CCTGCACCGC AGATGGAGAA 
GAAGAGGAAG GTAGAGAGGC TGAAGATGGC CCGGTGGAGG AGTTCAAGCC CAAGCCCAGG 
CCCTTCATGC CCAACTTGGT GCCGCCCAAG ATCCCTGATG GAGAGAGGGT GGACTTCGAT 
GACATACACC GGAAGCGCAT GGAGAAGGAC CTCAACGAGC TGCAGACACT GATCGAGGCG 
CATTTCGAGA ACCGCAAGAA GGAGGAGGAG GAGCTGGTCT CCCTCAAAGA CAGGATCGAG 
AAGCGGCGGG CAGAACGCGC GGAGCAGCAG CGCATCCGCG CGGAGCGCGA GAAGGAGCGG 
CAGACGCGCC TGGCGGAGGA GCGAGCCCGC CGAGAGGAGG AGGAGAGCCG CAGGAAGGCG 
GAGGACGAGG CGCGCAAGAA GAAGGCTCTG TCCAACATGA TGCACTTCGG CGGCTACATC 
CAGAAGGCCC AGACAGAGCG TAAAAGTGGG AAGAGACAGA CCGAGCGGGA GAAGAAGAAG 
AAGATTCTGG CCGAGCGGAG GAAGGTGCTG GCCATCGACC ACCTGAACGA AGACCAGCTG 
AGGGAGAAGG CCAAGGAGCT GTGGCAGAAC ATCTACGACC TGGAGGCGGA GAAGTTCGAC 
CTGCAAGAGA AGTTCAAGCA GCAGAAATAC GAGATCAATG TTCTCCGAAA CAGGATCAAT 
GACAACCAGA AAGTCTCCAA GACTCGAGGG AAGGCCAAGG TCACGGGGCG CTGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNT2 ( XM_024976060.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831828.1 Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa223443
Clone ID Related Accession (Same CDS sequence)	XM_024976060.1
Accession Version	XM_024976060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	troponin T, cardiac muscle isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAGCA CGAGGAGGCG 
GTGGAAGAGG AGGCTGGAGG CGAGGCTGAG GCTGGGGAGC CCTGCACCGC AGAAGATGGA 
GAAGAAGAGG AAGGTAGAGA GGCTGAAGAT GGCCCGGTGG AGGAGTTCAA GCCCAAGCCC 
AGGCCCTTCA TGCCCAACTT GGTGCCGCCC AAGATCCCTG ATGGAGAGAG GGTGGACTTC 
GATGACATAC ACCGGAAGCG CATGGAGAAG GACCTCAACG AGCTGCAGAC ACTGATCGAG 
GCGCATTTCG AGAACCGCAA GAAGGAGGAG GAGGAGCTGG TCTCCCTCAA AGACAGGATC 
GAGAAGCGGC GGGCAGAACG CGCGGAGCAG CAGCGCATCC GCGCGGAGCG CGAGAAGGAG 
CGGCAGACGC GCCTGGCGGA GGAGCGAGCC CGCCGAGAGG AGGAGGAGAG CCGCAGGAAG 
GCGGAGGACG AGGCGCGCAA GAAGAAGGCT CTGTCCAACA TGATGCACTT CGGCGGCTAC 
ATCCAGAAGA CAGAGCGTAA AAGTGGGAAG AGACAGACCG AGCGGGAGAA GAAGAAGAAG 
ATTCTGGCCG AGCGGAGGAA GGTGCTGGCC ATCGACCACC TGAACGAAGA CCAGCTGAGG 
GAGAAGGCCA AGGAGCTGTG GCAGAACATC TACGACCTGG AGGCGGAGAA GTTCGACCTG 
CAAGAGAAGT TCAAGCAGCA GAAATACGAG ATCAATGTTC TCCGAAACAG GATCAATGAC 
AACCAGAAAG TCTCCAAGAC TCGAGGGAAG GCCAAGGTCA CGGGGCGCTG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831828.1
CDS	105..941
Translation	MSDVEEAVEEYEEQEEHEEAVEEEAGGEAEAGEPCTAEDGEEEEGREAEDGPVEEFKPKPRPFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQTLIEAHFENRKKEEEELVSLKDRIEKRRAERAEQQRIRAEREKERQTRLAEERARREEEESRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQNIYDLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Target ORF information:

RefSeq Version	XM_024976060.1
Organism	Bos taurus(cattle)
Definition	Bos taurus troponin T2, cardiac type (TNNT2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976060.1

ORF Insert Sequence:

ATGTCGGACG TGGAAGAGGC CGTGGAGGAG TATGAGGAGC AGGAAGAGCA CGAGGAGGCG 
GTGGAAGAGG AGGCTGGAGG CGAGGCTGAG GCTGGGGAGC CCTGCACCGC AGAAGATGGA 
GAAGAAGAGG AAGGTAGAGA GGCTGAAGAT GGCCCGGTGG AGGAGTTCAA GCCCAAGCCC 
AGGCCCTTCA TGCCCAACTT GGTGCCGCCC AAGATCCCTG ATGGAGAGAG GGTGGACTTC 
GATGACATAC ACCGGAAGCG CATGGAGAAG GACCTCAACG AGCTGCAGAC ACTGATCGAG 
GCGCATTTCG AGAACCGCAA GAAGGAGGAG GAGGAGCTGG TCTCCCTCAA AGACAGGATC 
GAGAAGCGGC GGGCAGAACG CGCGGAGCAG CAGCGCATCC GCGCGGAGCG CGAGAAGGAG 
CGGCAGACGC GCCTGGCGGA GGAGCGAGCC CGCCGAGAGG AGGAGGAGAG CCGCAGGAAG 
GCGGAGGACG AGGCGCGCAA GAAGAAGGCT CTGTCCAACA TGATGCACTT CGGCGGCTAC 
ATCCAGAAGA CAGAGCGTAA AAGTGGGAAG AGACAGACCG AGCGGGAGAA GAAGAAGAAG 
ATTCTGGCCG AGCGGAGGAA GGTGCTGGCC ATCGACCACC TGAACGAAGA CCAGCTGAGG 
GAGAAGGCCA AGGAGCTGTG GCAGAACATC TACGACCTGG AGGCGGAGAA GTTCGACCTG 
CAAGAGAAGT TCAAGCAGCA GAAATACGAG ATCAATGTTC TCCGAAACAG GATCAATGAC 
AACCAGAAAG TCTCCAAGAC TCGAGGGAAG GCCAAGGTCA CGGGGCGCTG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FRET study of the structural and kinetic effects of PKC phosphomimetic cardiac troponin T mutants on thin filament regulation.
Archives of biochemistry and biophysics550-5511-11(2014 May)
Schlecht W,Zhou Z,Li KL,Rieck D,Ouyang Y,Dong WJ

Unexpected cross-species contamination in genome sequencing projects.
PeerJ2e675(2014)
Merchant S,Wood DE,Salzberg SL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage.
Biochemistry45(38)11681-94(2006 Sep)
Zhang Z,Biesiadecki BJ,Jin JP

Functional consequences of troponin T mutations found in hypertrophic cardiomyopathy.
The Journal of biological chemistry274(40)28363-70(1999 Oct)
Tobacman LS,Lin D,Butters C,Landis C,Back N,Pavlov D,Homsher E

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TNNT2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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