SPON1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPON1
Entrez Gene ID	282866
Full Name	spondin 1
General protein information	Preferred Names spondin 1 Names spondin-1 F-spondin spondin 1, (f-spondin) extracellular matrix protein spondin 1, extracellular matrix protein vascular smooth muscle cell growth-promoting factor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174743.2	NP_777168.1	spondin-1 precursor

Pathways from BioSystems

Homologies

	SPON1	XP_001093336.1
Mus musculus (house mouse)	Spon1	NP_663559.1
Xenopus tropicalis (tropical clawed frog)	spon1	NP_001090741.1
Homo sapiens (human)	SPON1	NP_006099.2
Rattus norvegicus (Norway rat)	Spon1	NP_742064.1
Gallus gallus (chicken)	SPON1	NP_990182.1
Danio rerio (zebrafish)	spon1a	NP_571590.1
Drosophila melanogaster (fruit fly)	CG17739	NP_610757.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012307	XP_320234.3
Pan troglodytes (chimpanzee)	SPON1	XP_508295.3
Canis lupus familiaris (dog)	SPON1	XP_534067.2
Bos taurus (cattle)	SPON1	NP_777168.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Isolation and characterization of vascular smooth muscle cell growth promoting factor from bovine ovarian follicular fluid and its cDNA cloning from bovine and human ovary.
Miyamoto K, Morishita Y, Yamazaki M, Minamino N, Kangawa K, Matsuo H, Mizutani T, Yamada K, Minegishi T
Archives of biochemistry and biophysics390(1)93-100(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPON1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPON1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42814	NM_174743.2 Latest version!	Bos taurus spondin 1 (SPON1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPON1 ( NM_174743.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777168.1 Bos taurus spondin 1 (SPON1), mRNA.

Price & Availability↑

CloneID	OBa42814
Clone ID Related Accession (Same CDS sequence)	NM_174743.2
Accession Version	NM_174743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	spondin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB051389.1. On or before Sep 16, 2007 this sequence version replaced XM_001253412.1, NM_174743.1. ##Evidence-Data-START## Transcript exon combination :: AB051389.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGGCTGT CCCCGGTGCT CCTGAGGCTG AGCCGGGGTC CAGCGCTGCT GGCCCTGGCG 
CTGCCCCTGG CGGTGGCGCT GGCCTTCTCC GACGAGACCC TGGACAAAGT GCCCAAGTCA 
GAGGGCTACT GCAGCCGCAT CCTGCGCGTC CAGGGCACGC GGCGTGAGGG CTACACCGAG 
TTCAGCCTCC GCGTGGAGGG CGACCCCGAC TTCTATAAGC CGGGAACCAG CTACCGCGTG 
ACGCTTTCAG CTGCCCCTCC CTCCTATTTC AGAGGATTCA CCTTAATCGC CCTCAAAGAA 
AACAGAGAGG GTGATAAAGA GGAAGACCAT GCTGGGACCT TCCAGATCAT AGATGAAGAA 
GAAACACAGT TTATGAGTAA TTGCCCCGTT GCGGTCACAG AAAGCACCCC ACGGAGGCGG 
ACGCGGATCC AGGTGTTCTG GATAGCGCCA CCTGCAGGAA CTGGCTGCGT AATTCTAAAG 
GCTAGCATTG TACAGAAACG CATCATTTAT TTTCAAGATG AAGGCTCTCT GACCAAGAAA 
CTTTGTGAAC AAGATTCCAC ATTTGATGGG GTGACTGACA AACCAATCCT AGACTGCTGC 
GCCTGTGGGA CTGCTAAGTA CAGGCTCACA TTTTATGGGA ACTGGTCTGA GAAGACACAC 
CCAAAGGATT ACCCTCGTCG GGCCAATCAC TGGTCTGCAA TCATCGGCGG ATCCCACTCC 
AAGAATTACG TGCTGTGGGA GTATGGAGGA TATGCAAGCG AAGGCGTCAA ACAGGTTGCA 
GAATTGGGCT CTCCAGTGAA AATGGAGGAG GAAATTCGAC AACAGAGCGA CGAGGTCCTC 
ACCGTCATCA AAGCCAAAGC TCAGTGGCCA GCCTGGCAGC CTCTCAATGT GAGAGCAGCA 
CCCTCGGCTG AATTTTCAGT GGACAGGACG CGCCACTTGA TGTCCTTCCT GACCATGATG 
GGCCCCAGCC CCGACTGGAA CGTGGGCCTG TCCGCAGAGG ATCTGTGCAC CAAAGAGTGC 
GGCTGGGTCC AGAAGGTGGT GCAGGACCTG ATTCCCTGGG ACGCGGGCAC CGACAGCGGG 
GTGACCTATG AGTCACCCAA CAAGCCCACA ATTCCCCAGG AGAAAATCCG ACCCCTGACC 
AGTCTGGACC ATCCTCAGAG TCCTTTTTAT GATCCAGAGG GTGGATCCAT CACTCAAGTA 
GCCAGAGTTG TCATCGAGAG AATCGCCCGG AAGGGGGAAC AGTGCAATAT TGTGCCCGAC 
AATGTCGACG ATATTGTAGC AGACCTGGCT CCAGAAGAGA AAGATGAAGA TGACACCCCT 
GAAACCTGCA TCTACTCCAA CTGGTCCCCG TGGTCCGCCT GCAGCTCCTC CACCTGCGAC 
AAAGGCAAGA GGATGCGGCA GCGCATGCTG AAGGCACAGC TGGACCTCAG CGTCCCCTGC 
CCCGACACCC AGGACTTCCA GCCCTGCATG GGCCCCGGCT GCAGCGACGA AGACGGCTCC 
ACGTGCACCA TGTCCGAGTG GATCACCTGG TCGCCCTGCA GCATCTCCTG CGGCACCGGC 
ACGCGGTCCC GGGAGAGGTA CGTGAAGCAG TTCCCAGAGG ACGGCTCCGT GTGCACGCTG 
CCCACCGAGG AGACAGAGAA GTGCACGGTC AACGAAGAGT GCTCTCCCAG CAGCTGCCTG 
ACGACCGAGT GGGGCGAGTG GGACGAGTGC AGCGCCACCT GCGGGATGGG CATGAAGAAG 
CGGCACCGCA TGGTCAAGAT GAGCCCCGCG GACGGCTCCA TGTGCAAGGC TGAAACATCT 
CAGGCAGAGA AGTGCATGAT GCCCGAGTGC CACACCATCC CATGCTTGCT ATCCCTGTGG 
TCTGAGTGGA GTGACTGCAG CGTGACCTGT GGGAAGGGCA TGCGGACCCG CCAGCGGATG 
CTCAAGTCTC TAGCCGAACT CGGGGACTGT AATGAGGAGC TGGAGCAGGT GGAGAAGTGC 
ATGCTGCCTG AATGCCCCAT TGACTGTGAG CTCACGGAGT GGTCCCAGTG GTCGGAATGT 
AACAAGTCAT GTGGGAAAGG ACACATGATT CGAACCCGCA TGATCCAAAT GGAGCCTCAG 
TTTGGAGGCA CACCCTGCCC AGAGACTGTA CAGCGGAAAA AGTGCCGCAT CCGGAAATGC 
CTCCGAAATC CATCCATCCA GAACCTGCGC TGGAGGGAGG CCCGAGAGAG CCGGAGGAGT 
GAACAGCTGA GGGAGGAGTC GGACGGGGAC CAGTTTCCAG GCTGCAGGAT GCGCCCTTGG 
ACAGCCTGGT CAGAATGCAC CAAACTGTGC GGAGGAGGGA TCCAGGAACG CTACATGACT 
GTGAAGAAGA GGTTCAAAAG CTCCCAGTTT ACCAGCTGCA AAGACAAGAA GGAGATCAGA 
GCGTGCAACG TCCATCCGTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777168.1
CDS	127..2550
Translation	MRLSPVLLRLSRGPALLALALPLAVALAFSDETLDKVPKSEGYCSRILRVQGTRREGYTEFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALKENREGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGTGTRSRERYVKQFPEDGSVCTLPTEETEKCTVNEECSPSSCLTTEWGEWDECSATCGMGMKKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTIPCLLSLWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNEELEQVEKCMLPECPIDCELTEWSQWSECNKSCGKGHMIRTRMIQMEPQFGGTPCPETVQRKKCRIRKCLRNPSIQNLRWREARESRRSEQLREESDGDQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKEIRACNVHPC

Target ORF information:

RefSeq Version	NM_174743.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spondin 1 (SPON1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174743.2

ORF Insert Sequence:

ATGAGGCTGT CCCCGGTGCT CCTGAGGCTG AGCCGGGGTC CAGCGCTGCT GGCCCTGGCG 
CTGCCCCTGG CGGTGGCGCT GGCCTTCTCC GACGAGACCC TGGACAAAGT GCCCAAGTCA 
GAGGGCTACT GCAGCCGCAT CCTGCGCGTC CAGGGCACGC GGCGTGAGGG CTACACCGAG 
TTCAGCCTCC GCGTGGAGGG CGACCCCGAC TTCTATAAGC CGGGAACCAG CTACCGCGTG 
ACGCTTTCAG CTGCCCCTCC CTCCTATTTC AGAGGATTCA CCTTAATCGC CCTCAAAGAA 
AACAGAGAGG GTGATAAAGA GGAAGACCAT GCTGGGACCT TCCAGATCAT AGATGAAGAA 
GAAACACAGT TTATGAGTAA TTGCCCCGTT GCGGTCACAG AAAGCACCCC ACGGAGGCGG 
ACGCGGATCC AGGTGTTCTG GATAGCGCCA CCTGCAGGAA CTGGCTGCGT AATTCTAAAG 
GCTAGCATTG TACAGAAACG CATCATTTAT TTTCAAGATG AAGGCTCTCT GACCAAGAAA 
CTTTGTGAAC AAGATTCCAC ATTTGATGGG GTGACTGACA AACCAATCCT AGACTGCTGC 
GCCTGTGGGA CTGCTAAGTA CAGGCTCACA TTTTATGGGA ACTGGTCTGA GAAGACACAC 
CCAAAGGATT ACCCTCGTCG GGCCAATCAC TGGTCTGCAA TCATCGGCGG ATCCCACTCC 
AAGAATTACG TGCTGTGGGA GTATGGAGGA TATGCAAGCG AAGGCGTCAA ACAGGTTGCA 
GAATTGGGCT CTCCAGTGAA AATGGAGGAG GAAATTCGAC AACAGAGCGA CGAGGTCCTC 
ACCGTCATCA AAGCCAAAGC TCAGTGGCCA GCCTGGCAGC CTCTCAATGT GAGAGCAGCA 
CCCTCGGCTG AATTTTCAGT GGACAGGACG CGCCACTTGA TGTCCTTCCT GACCATGATG 
GGCCCCAGCC CCGACTGGAA CGTGGGCCTG TCCGCAGAGG ATCTGTGCAC CAAAGAGTGC 
GGCTGGGTCC AGAAGGTGGT GCAGGACCTG ATTCCCTGGG ACGCGGGCAC CGACAGCGGG 
GTGACCTATG AGTCACCCAA CAAGCCCACA ATTCCCCAGG AGAAAATCCG ACCCCTGACC 
AGTCTGGACC ATCCTCAGAG TCCTTTTTAT GATCCAGAGG GTGGATCCAT CACTCAAGTA 
GCCAGAGTTG TCATCGAGAG AATCGCCCGG AAGGGGGAAC AGTGCAATAT TGTGCCCGAC 
AATGTCGACG ATATTGTAGC AGACCTGGCT CCAGAAGAGA AAGATGAAGA TGACACCCCT 
GAAACCTGCA TCTACTCCAA CTGGTCCCCG TGGTCCGCCT GCAGCTCCTC CACCTGCGAC 
AAAGGCAAGA GGATGCGGCA GCGCATGCTG AAGGCACAGC TGGACCTCAG CGTCCCCTGC 
CCCGACACCC AGGACTTCCA GCCCTGCATG GGCCCCGGCT GCAGCGACGA AGACGGCTCC 
ACGTGCACCA TGTCCGAGTG GATCACCTGG TCGCCCTGCA GCATCTCCTG CGGCACCGGC 
ACGCGGTCCC GGGAGAGGTA CGTGAAGCAG TTCCCAGAGG ACGGCTCCGT GTGCACGCTG 
CCCACCGAGG AGACAGAGAA GTGCACGGTC AACGAAGAGT GCTCTCCCAG CAGCTGCCTG 
ACGACCGAGT GGGGCGAGTG GGACGAGTGC AGCGCCACCT GCGGGATGGG CATGAAGAAG 
CGGCACCGCA TGGTCAAGAT GAGCCCCGCG GACGGCTCCA TGTGCAAGGC TGAAACATCT 
CAGGCAGAGA AGTGCATGAT GCCCGAGTGC CACACCATCC CATGCTTGCT ATCCCTGTGG 
TCTGAGTGGA GTGACTGCAG CGTGACCTGT GGGAAGGGCA TGCGGACCCG CCAGCGGATG 
CTCAAGTCTC TAGCCGAACT CGGGGACTGT AATGAGGAGC TGGAGCAGGT GGAGAAGTGC 
ATGCTGCCTG AATGCCCCAT TGACTGTGAG CTCACGGAGT GGTCCCAGTG GTCGGAATGT 
AACAAGTCAT GTGGGAAAGG ACACATGATT CGAACCCGCA TGATCCAAAT GGAGCCTCAG 
TTTGGAGGCA CACCCTGCCC AGAGACTGTA CAGCGGAAAA AGTGCCGCAT CCGGAAATGC 
CTCCGAAATC CATCCATCCA GAACCTGCGC TGGAGGGAGG CCCGAGAGAG CCGGAGGAGT 
GAACAGCTGA GGGAGGAGTC GGACGGGGAC CAGTTTCCAG GCTGCAGGAT GCGCCCTTGG 
ACAGCCTGGT CAGAATGCAC CAAACTGTGC GGAGGAGGGA TCCAGGAACG CTACATGACT 
GTGAAGAAGA GGTTCAAAAG CTCCCAGTTT ACCAGCTGCA AAGACAAGAA GGAGATCAGA 
GCGTGCAACG TCCATCCGTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Isolation and characterization of vascular smooth muscle cell growth promoting factor from bovine ovarian follicular fluid and its cDNA cloning from bovine and human ovary.
Archives of biochemistry and biophysics390(1)93-100(2001 Jun)
Miyamoto K,Morishita Y,Yamazaki M,Minamino N,Kangawa K,Matsuo H,Mizutani T,Yamada K,Minegishi T

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SPON1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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