GRK2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK2
Entrez Gene ID	282682
Full Name	G protein-coupled receptor kinase 2
Synonyms	ADRBK1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174710.2	NP_777135.1	beta-adrenergic receptor kinase 1
XM_024986987.1	XP_024842755.1	beta-adrenergic receptor kinase 1 isoform X1

Pathways from BioSystems

Homologies

	ADRBK1	XP_001117955.2
Bos taurus (cattle)	ADRBK1	NP_777135.1
Pan troglodytes (chimpanzee)	ADRBK1	XP_508589.3
Canis lupus familiaris (dog)	ADRBK1	XP_852085.1
Mus musculus (house mouse)	Adrbk1	NP_570933.1
Homo sapiens (human)	ADRBK1	NP_001610.2
Rattus norvegicus (Norway rat)	Adrbk1	NP_036908.1
Gallus gallus (chicken)	ADRBK1	NP_001026524.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of Lipid Composition on the Membrane Orientation of the G Protein-Coupled Receptor Kinase 2-G?1?2 Complex.
Yang P, Homan KT, Li Y, Cruz-Rodr?guez O, Tesmer JJ, Chen Z
Biochemistry55(20)2841-8(2016 05)

Prodeath signaling of G protein-coupled receptor kinase 2 in cardiac myocytes after ischemic stress occurs via extracellular signal-regulated kinase-dependent heat shock protein 90-mediated mitochondrial targeting.
Chen M, Sato PY, Chuprun JK, Peroutka RJ, Otis NJ, Ibetti J, Pan S, Sheu SS, Gao E, Koch WJ
Circulation research112(8)1121-34(2013 Apr)

Growth inhibition of human hepatocellular carcinoma cells by overexpression of G-protein-coupled receptor kinase 2.
Wei Z, Hurtt R, Ciccarelli M, Koch WJ, Doria C
Journal of cellular physiology227(6)2371-7(2012 Jun)

A novel GRK2/HDAC6 interaction modulates cell spreading and motility.
Lafarga V, Aymerich I, Tapia O, Mayor F Jr, Penela P
The EMBO journal31(4)856-69(2012 Feb)

Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
Evron T, Philipp M, Lu J, Meloni AR, Burkhalter M, Chen W, Caron MG
The Journal of biological chemistry286(31)27676-86(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The PIP2-induced orientation of the GRK2-Gbeta1gamma2 complex is therefore most likely caused by specific interactions between PIP2 and the GRK2 PH domain.
Title: Effect of Lipid Composition on the Membrane Orientation of the G Protein-Coupled Receptor Kinase 2-G?1?2 Complex.

Mitochondrial-targeted GRK2 is essential for prodeath signaling occurring after oxidative stress in myocytes and assigning a novel role for this GRK.
Title: Prodeath signaling of G protein-coupled receptor kinase 2 in cardiac myocytes after ischemic stress occurs via extracellular signal-regulated kinase-dependent heat shock protein 90-mediated mitochondrial targeting.

Indicate that the anti-proliferative function of elevated GRK2 in hepatocellular carcinoma is associated with delayed cell cycle progression and is GRK2 kinase activity-dependent.
Title: Growth inhibition of human hepatocellular carcinoma cells by overexpression of G-protein-coupled receptor kinase 2.

G protein-coupled receptor kinase 2 plays a positive role in Smoothened signaling, at least in part, through the promotion of an association between beta-arrestin 2 and Smoothened
Title: Smoothened signal transduction is promoted by G protein-coupled receptor kinase 2.

atomic structure of GRK2 in complex with Galphaq and Gbetagamma, in which activated Galpha subunit of Gq is fully dissociated from Gbetagamma and dramatically reoriented from its position in the inactive Galphabetagamma heterotrimer [Galphaq, Gbetagamma
Title: Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex.

The following GRK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01398	NM_174710.2 Latest version!	Bos taurus G protein-coupled receptor kinase 2 (GRK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa231844	XM_024986987.1 Latest version!	Bos taurus G protein-coupled receptor kinase 2 (GRK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK2 ( NM_174710.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777135.1 Bos taurus G protein-coupled receptor kinase 2 (GRK2), mRNA.

Price & Availability↑

CloneID	OBa01398
Clone ID Related Accession (Same CDS sequence)	NM_174710.2
Accession Version	NM_174710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M34019.1. On Jun 3, 2003 this sequence version replaced NM_174710.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M34019.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCGGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGCGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CTTTTGAGAA GATCTTCTCC 
CAGAAGCTGG GGTACCTGCT TTTCCGAGAC TTCTGCCTGA AGCACCTGGA GGAGGCCAAG 
CCCTTGGTAG AGTTCTACGA GGAGATCAAG AAATACGAGA AGCTGGAGAC AGAGGAGGAG 
CGCCTGGTCT GCAGCCGAGA GATCTTCGAC ACGTACATCA TGAAGGAGCT GCTGGCCTGC 
TCACATCCTT TCTCGAAGAG CGCCATTGAG CACGTCCAGG GCCATCTGGT GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATATATT GAAGAAATTT GCCAGAACCT CCGAGGAGAC 
GTGTTCCAGA AATTCATCGA GAGCGATAAA TTCACACGAT TTTGCCAGTG GAAGAATGTA 
GAGCTCAACA TCCACCTGAC CATGAACGAC TTCAGTGTGC ACCGCATCAT CGGGCGAGGC 
GGCTTCGGTG AGGTCTACGG CTGCCGGAAG GCCGACACGG GCAAGATGTA CGCCATGAAG 
TGTCTGGACA AGAAGCGCAT CAAGATGAAG CAAGGGGAGA CTCTGGCCCT GAATGAGCGC 
ATCATGCTGT CGCTCGTCAG CACCGGGGAC TGCCCGTTCA TCGTCTGCAT GTCATACGCC 
TTCCACACAC CGGACAAGCT CAGCTTCATC CTGGATCTCA TGAACGGCGG GGACCTGCAC 
TACCACCTGT CCCAGCACGG GGTCTTCTCC GAGGCCGACA TGCGTTTCTA CGCCGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCG 
GCCAACATCC TGCTGGACGA GCACGGCCAC GTGCGCATCT CAGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC TCACGCCAGT GTGGGCACCC ACGGGTACAT GGCTCCCGAG 
GTTCTACAGA AGGGTGTGGC CTACGACAGC AGCGCCGACT GGTTCTCCCT GGGCTGCATG 
CTCTTCAAGC TGCTGCGAGG GCATAGCCCT TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACA GAATGACATT GACAATGGCT GTGGAGCTGC CTGACTCCTT CTCCCCTGAG 
CTCCGCTCCT TGCTGGAGGG GCTGCTGCAG AGGGATGTCA ACCGGAGGCT AGGCTGCCTG 
GGCCGAGGGG CCCAGGAGGT GAAGGAGAGC CCCTTCTTCC GTTCCCTGGA CTGGCAGATG 
GTCTTTTTAC AAAAGTACCC TCCCCCGTTG ATCCCCCCAC GAGGGGAGGT GAATGCAGCC 
GACGCCTTTG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GCTACTGGAC 
AGTGACCAGG AGCTCTACCG CAACTTCCCC CTGACCATCT CGGAGCGGTG GCAGCAGGAG 
GTAGCAGAGA CTGTCTTTGA CACCATCAAT GCTGAGACGG ACCGGCTGGA GGCCCGCAAG 
AAAACCAAAA ACAAGCAGTT GGGCCACGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCTAACCG GCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG AAAGTGCCTC 
CTCCTCAAGA TCCGAGGTGG CAAGCAGTTT GTCCTGCAGT GCGATAGTGA CCCAGAGCTG 
GTGCAGTGGA AGAAGGAGCT TCGAGACGCC TACCGCGAGG CCCAGCAGCT AGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTCGTGG AGCTGAGCAA GGTGCCACTG 
ATCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777135.1
CDS	85..2154
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLKHLEEAKPLVEFYEEIKKYEKLETEEERLVCSREIFDTYIMKELLACSHPFSKSAIEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKTKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLIQRGSANGL

Target ORF information:

RefSeq Version	NM_174710.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 2 (GRK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174710.2

ORF Insert Sequence:

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCGGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGCGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CTTTTGAGAA GATCTTCTCC 
CAGAAGCTGG GGTACCTGCT TTTCCGAGAC TTCTGCCTGA AGCACCTGGA GGAGGCCAAG 
CCCTTGGTAG AGTTCTACGA GGAGATCAAG AAATACGAGA AGCTGGAGAC AGAGGAGGAG 
CGCCTGGTCT GCAGCCGAGA GATCTTCGAC ACGTACATCA TGAAGGAGCT GCTGGCCTGC 
TCACATCCTT TCTCGAAGAG CGCCATTGAG CACGTCCAGG GCCATCTGGT GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATATATT GAAGAAATTT GCCAGAACCT CCGAGGAGAC 
GTGTTCCAGA AATTCATCGA GAGCGATAAA TTCACACGAT TTTGCCAGTG GAAGAATGTA 
GAGCTCAACA TCCACCTGAC CATGAACGAC TTCAGTGTGC ACCGCATCAT CGGGCGAGGC 
GGCTTCGGTG AGGTCTACGG CTGCCGGAAG GCCGACACGG GCAAGATGTA CGCCATGAAG 
TGTCTGGACA AGAAGCGCAT CAAGATGAAG CAAGGGGAGA CTCTGGCCCT GAATGAGCGC 
ATCATGCTGT CGCTCGTCAG CACCGGGGAC TGCCCGTTCA TCGTCTGCAT GTCATACGCC 
TTCCACACAC CGGACAAGCT CAGCTTCATC CTGGATCTCA TGAACGGCGG GGACCTGCAC 
TACCACCTGT CCCAGCACGG GGTCTTCTCC GAGGCCGACA TGCGTTTCTA CGCCGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCG 
GCCAACATCC TGCTGGACGA GCACGGCCAC GTGCGCATCT CAGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC TCACGCCAGT GTGGGCACCC ACGGGTACAT GGCTCCCGAG 
GTTCTACAGA AGGGTGTGGC CTACGACAGC AGCGCCGACT GGTTCTCCCT GGGCTGCATG 
CTCTTCAAGC TGCTGCGAGG GCATAGCCCT TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACA GAATGACATT GACAATGGCT GTGGAGCTGC CTGACTCCTT CTCCCCTGAG 
CTCCGCTCCT TGCTGGAGGG GCTGCTGCAG AGGGATGTCA ACCGGAGGCT AGGCTGCCTG 
GGCCGAGGGG CCCAGGAGGT GAAGGAGAGC CCCTTCTTCC GTTCCCTGGA CTGGCAGATG 
GTCTTTTTAC AAAAGTACCC TCCCCCGTTG ATCCCCCCAC GAGGGGAGGT GAATGCAGCC 
GACGCCTTTG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GCTACTGGAC 
AGTGACCAGG AGCTCTACCG CAACTTCCCC CTGACCATCT CGGAGCGGTG GCAGCAGGAG 
GTAGCAGAGA CTGTCTTTGA CACCATCAAT GCTGAGACGG ACCGGCTGGA GGCCCGCAAG 
AAAACCAAAA ACAAGCAGTT GGGCCACGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCTAACCG GCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG AAAGTGCCTC 
CTCCTCAAGA TCCGAGGTGG CAAGCAGTTT GTCCTGCAGT GCGATAGTGA CCCAGAGCTG 
GTGCAGTGGA AGAAGGAGCT TCGAGACGCC TACCGCGAGG CCCAGCAGCT AGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTCGTGG AGCTGAGCAA GGTGCCACTG 
ATCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK2 ( XM_024986987.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842755.1 Bos taurus G protein-coupled receptor kinase 2 (GRK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231844
Clone ID Related Accession (Same CDS sequence)	XM_024986987.1
Accession Version	XM_024986987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCGGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGCGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CTTTTGAGAA GATCTTCTCC 
CAGAAGCTGG GGTACCTGCT TTTCCGAGAC TTCTGCCTGA AGCACCTGGA GGAGGCCAAG 
CCCTTGGTAG AGTTCTACGA GGAGATCAAG AAATACGAGA AGCTGGAGAC AGAGGAGGAG 
CGCCTGGTCT GCAGCCGAGA GATCTTCGAC ACGTACATCA TGAAGGAGCT GCTGGCCTGC 
TCACATCCTT TCTCGAAGAG CGCCATTGAG CACGTCCAGG GCCATCTGGT GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATATATT GAAGAAATTT GCCAGAACCT CCGAGGAGAC 
GTGTTCCAGA AATTCATCGA GAGCGATAAA TTCACACGAT TTTGCCAGTG GAAGAATGTA 
GAGCTCAACA TCCACCTGAC CATGAACGAC TTCAGTGTGC ACCGCATCAT CGGGCGAGGC 
GGCTTCGGTG AGGTCTACGG CTGCCGGAAG GCCGACACGG GCAAGATGTA CGCCATGAAG 
TGTCTGGACA AGAAGCGCAT CAAGATGAAG CAAGGGGAGA CTCTGGCCCT GAATGAGCGC 
ATCATGCTGT CGCTCGTCAG CACCGGGGAC TGCCCGTTCA TCGTCTGCAT GTCATACGCC 
TTCCACACAC CGGACAAGCT CAGCTTCATC CTGGATCTCA TGAACGGCGG GGACCTGCAC 
TACCACCTGT CCCAGCACGG GGTCTTCTCC GAGGCCGACA TGCGTTTCTA CGCCGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCG 
GCCAACATCC TGCTGGACGA GCACGGCCAC GTGCGCATCT CAGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC TCACGCCAGT GTGGGCACCC ACGGGTACAT GGCTCCCGAG 
GTTCTACAGA AGGGTGTGGC CTACGACAGC AGCGCCGACT GGTTCTCCCT GGGCTGCATG 
CTCTTCAAGC TGCTGCGAGG GCATAGCCCT TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACA GAATGACATT GACAATGGCT GTGGAGCTGC CTGACTCCTT CTCCCCTGAG 
CTCCGCTCCT TGCTGGAGGG GCTGCTGCAG AGGGATGTCA ACCGGAGGCT AGGCTGCCTG 
GGCCGAGGGG CCCAGGAGGT GAAGGAGAGC CCCTTCTTCC GTTCCCTGGA CTGGCAGATG 
GTCTTTTTAC AAAAGTACCC TCCCCCGTTG ATCCCCCCAC GAGGGGAGGT GAATGCAGCC 
GACGCCTTTG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GCTACTGGAC 
AGTGACCAGG AGCTCTACCG CAACTTCCCC CTGACCATCT CGGAGCGGTG GCAGCAGGAG 
GTAGCAGAGA CTGTCTTTGA CACCATCAAT GCTGAGACGG ACCGGCTGGA GGCCCGCAAG 
AAAACCAAAA ACAAGCAGTT GGGCCACGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCTAACCG GCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GAGCCTGCTG 
ACCATGGAGG AGATCCAGTC GGTGGAGGAG ACGCAGATCA AGGAGCGAAA GTGCCTCCTC 
CTCAAGATCC GAGGTGGCAA GCAGTTTGTC CTGCAGTGCG ATAGTGACCC AGAGCTGGTG 
CAGTGGAAGA AGGAGCTTCG AGACGCCTAC CGCGAGGCCC AGCAGCTAGT GCAGCGGGTG 
CCCAAGATGA AGAACAAGCC GCGCTCGCCC GTCGTGGAGC TGAGCAAGGT GCCACTGATC 
CAGCGCGGCA GTGCCAACGG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842755.1
CDS	220..2286
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLKHLEEAKPLVEFYEEIKKYEKLETEEERLVCSREIFDTYIMKELLACSHPFSKSAIEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKTKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLIQRGSANGL

Target ORF information:

RefSeq Version	XM_024986987.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 2 (GRK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986987.1

ORF Insert Sequence:

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCGGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGCGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CTTTTGAGAA GATCTTCTCC 
CAGAAGCTGG GGTACCTGCT TTTCCGAGAC TTCTGCCTGA AGCACCTGGA GGAGGCCAAG 
CCCTTGGTAG AGTTCTACGA GGAGATCAAG AAATACGAGA AGCTGGAGAC AGAGGAGGAG 
CGCCTGGTCT GCAGCCGAGA GATCTTCGAC ACGTACATCA TGAAGGAGCT GCTGGCCTGC 
TCACATCCTT TCTCGAAGAG CGCCATTGAG CACGTCCAGG GCCATCTGGT GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATATATT GAAGAAATTT GCCAGAACCT CCGAGGAGAC 
GTGTTCCAGA AATTCATCGA GAGCGATAAA TTCACACGAT TTTGCCAGTG GAAGAATGTA 
GAGCTCAACA TCCACCTGAC CATGAACGAC TTCAGTGTGC ACCGCATCAT CGGGCGAGGC 
GGCTTCGGTG AGGTCTACGG CTGCCGGAAG GCCGACACGG GCAAGATGTA CGCCATGAAG 
TGTCTGGACA AGAAGCGCAT CAAGATGAAG CAAGGGGAGA CTCTGGCCCT GAATGAGCGC 
ATCATGCTGT CGCTCGTCAG CACCGGGGAC TGCCCGTTCA TCGTCTGCAT GTCATACGCC 
TTCCACACAC CGGACAAGCT CAGCTTCATC CTGGATCTCA TGAACGGCGG GGACCTGCAC 
TACCACCTGT CCCAGCACGG GGTCTTCTCC GAGGCCGACA TGCGTTTCTA CGCCGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCG 
GCCAACATCC TGCTGGACGA GCACGGCCAC GTGCGCATCT CAGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC TCACGCCAGT GTGGGCACCC ACGGGTACAT GGCTCCCGAG 
GTTCTACAGA AGGGTGTGGC CTACGACAGC AGCGCCGACT GGTTCTCCCT GGGCTGCATG 
CTCTTCAAGC TGCTGCGAGG GCATAGCCCT TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACA GAATGACATT GACAATGGCT GTGGAGCTGC CTGACTCCTT CTCCCCTGAG 
CTCCGCTCCT TGCTGGAGGG GCTGCTGCAG AGGGATGTCA ACCGGAGGCT AGGCTGCCTG 
GGCCGAGGGG CCCAGGAGGT GAAGGAGAGC CCCTTCTTCC GTTCCCTGGA CTGGCAGATG 
GTCTTTTTAC AAAAGTACCC TCCCCCGTTG ATCCCCCCAC GAGGGGAGGT GAATGCAGCC 
GACGCCTTTG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GCTACTGGAC 
AGTGACCAGG AGCTCTACCG CAACTTCCCC CTGACCATCT CGGAGCGGTG GCAGCAGGAG 
GTAGCAGAGA CTGTCTTTGA CACCATCAAT GCTGAGACGG ACCGGCTGGA GGCCCGCAAG 
AAAACCAAAA ACAAGCAGTT GGGCCACGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCTAACCG GCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GAGCCTGCTG 
ACCATGGAGG AGATCCAGTC GGTGGAGGAG ACGCAGATCA AGGAGCGAAA GTGCCTCCTC 
CTCAAGATCC GAGGTGGCAA GCAGTTTGTC CTGCAGTGCG ATAGTGACCC AGAGCTGGTG 
CAGTGGAAGA AGGAGCTTCG AGACGCCTAC CGCGAGGCCC AGCAGCTAGT GCAGCGGGTG 
CCCAAGATGA AGAACAAGCC GCGCTCGCCC GTCGTGGAGC TGAGCAAGGT GCCACTGATC 
CAGCGCGGCA GTGCCAACGG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of Lipid Composition on the Membrane Orientation of the G Protein-Coupled Receptor Kinase 2-G?1?2 Complex.
Biochemistry55(20)2841-8(2016 05)
Yang P,Homan KT,Li Y,Cruz-Rodr?guez O,Tesmer JJ,Chen Z

Prodeath signaling of G protein-coupled receptor kinase 2 in cardiac myocytes after ischemic stress occurs via extracellular signal-regulated kinase-dependent heat shock protein 90-mediated mitochondrial targeting.
Circulation research112(8)1121-34(2013 Apr)
Chen M,Sato PY,Chuprun JK,Peroutka RJ,Otis NJ,Ibetti J,Pan S,Sheu SS,Gao E,Koch WJ

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GRK2 cDNA ORF clone, Bos taurus(cattle)

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