VCAN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCAN
Entrez Gene ID	282662
Full Name	versican
Synonyms	CSPG2
General protein information	Preferred Names versican Names versican core protein GHAP PG-M chondroitin sulfate proteoglycan 2 (versican) chondroitin sulfate proteoglycan core protein 2 glial hyaluronate-binding protein large fibroblast proteoglycan
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015472358.1	XP_015327844.1	versican core protein isoform X3
NM_181035.2	NP_851378.1	versican core protein precursor
XM_015472358.2	XP_015327844.2	versican core protein isoform X3
XM_005209720.3	XP_005209777.1	versican core protein isoform X2
XM_005209719.4	XP_005209776.2	versican core protein isoform X1
XM_005209720.4	XP_005209777.2	versican core protein isoform X2
XM_005209719.3	XP_005209776.1	versican core protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	VCAN	NP_004376.2
Mus musculus (house mouse)	Vcan	NP_001074718.1
Bos taurus (cattle)	VCAN	NP_851378.1
	VCAN	XP_001112269.1
Canis lupus familiaris (dog)	VCAN	XP_003434465.1
Rattus norvegicus (Norway rat)	Vcan	NP_001164029.1
Pan troglodytes (chimpanzee)	VCAN	XP_517667.2

Gene Ontology
Bibliography

Related articles in PubMed

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.
Desmoulin GT, Hewitt CR, Hunter CJ
Spine36(14)E921-8(2011 Jun)

Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi.
Desmoulin GT, Reno CR, Hunter CJ
Spine35(15)1437-44(2010 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Versican V2 is a major extracellular matrix component of the mature bovine brain.
Schmalfeldt M, Dours-Zimmermann MT, Winterhalter KH, Zimmermann DR
The Journal of biological chemistry273(25)15758-64(1998 Jun)

Interaction of a brain extracellular matrix protein with hyaluronic acid.
Perides G, Biviano F, Bignami A
Biochimica et biophysica acta1075(3)248-58(1991 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that expression of mRNAs for aggrecan, collagen type II, and versican were significantly effected by the intervention.
Title: Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.

Data show that biglycan, collagen type I, collagen type II, decorin, and versican were significantly affected by vibration duration, frequency, and amplitude.
Title: Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi.

The following VCAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235783	XM_015472358.2 Latest version!	Bos taurus versican (VCAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa91173	XM_015472358.1 Latest version!	Bos taurus versican (VCAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa235782	XM_005209720.4 Latest version!	Bos taurus versican (VCAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa05606	XM_005209720.3 Latest version!	Bos taurus versican (VCAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa235781	XM_005209719.4 Latest version!	Bos taurus versican (VCAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa05605	XM_005209719.3 Latest version!	Bos taurus versican (VCAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa43820	NM_181035.2 Latest version!	Bos taurus versican (VCAN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCAN ( XM_015472358.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327844.2 Bos taurus versican (VCAN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa235783
Clone ID Related Accession (Same CDS sequence)	XM_015472358.2
Accession Version	XM_015472358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	versican core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGACCT GATCGTTGCA AAATGAACCC GTGCCTCAAT 
GGGGGCACCT GTTATCCTAC TGAAACTTCC TACGTGTGCA CCTGCGTGCC AGGATACAGT 
GGTGATCGGT GTGAACTTGA TTTTGATGAA TGTCATTCTA ATCCTTGTCG CAATGGAGCC 
ACATGTATTG ACGGTTTTAA TACCTTCAGG TGTCTCTGCC TCCCGAGCTA CGTTGGTGCG 
CTTTGTGAGC AAGACACGGA GACATGTGAC TATGGCTGGC ACAAATTTCA AGGGCAGTGC 
TACAAGTACT TTGCCCATCG ACGGACGTGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGCGCCCATC TCACCAGTAT CCTGTCTCAC GAGGAACAAA TGTTTGTGAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG TCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA TGAGAACTGG AGGCCGAACC AACCAGACAG CTTCTTTTCT 
ACTGGAGAAG ATTGTGTTGT GATTATATGG CATGAGAATG GCCAGTGGAA TGACGTTCCC 
TGCAATTACC ATCTCACCTA CACCTGCAAG AAAGGAACAG TTGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCTTG 
ATTAGATATC ACTGCAAAGA TGGTTTCATT CAACGCCACC TTCCAACTAT CCGTTGCCTA 
GGAAATGGAA GATGGGCTAT GCCTAAAATT ACCTGCCTGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA AGAAATATTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCAAAACATG ATCACCGTTG GAGCCGAAGG TGGCAAGAAT CAAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327844.2
CDS	296..2266
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPTPDSRFDAYCFKRPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	XM_015472358.2
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472358.2

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGACCT GATCGTTGCA AAATGAACCC GTGCCTCAAT 
GGGGGCACCT GTTATCCTAC TGAAACTTCC TACGTGTGCA CCTGCGTGCC AGGATACAGT 
GGTGATCGGT GTGAACTTGA TTTTGATGAA TGTCATTCTA ATCCTTGTCG CAATGGAGCC 
ACATGTATTG ACGGTTTTAA TACCTTCAGG TGTCTCTGCC TCCCGAGCTA CGTTGGTGCG 
CTTTGTGAGC AAGACACGGA GACATGTGAC TATGGCTGGC ACAAATTTCA AGGGCAGTGC 
TACAAGTACT TTGCCCATCG ACGGACGTGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGCGCCCATC TCACCAGTAT CCTGTCTCAC GAGGAACAAA TGTTTGTGAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG TCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA TGAGAACTGG AGGCCGAACC AACCAGACAG CTTCTTTTCT 
ACTGGAGAAG ATTGTGTTGT GATTATATGG CATGAGAATG GCCAGTGGAA TGACGTTCCC 
TGCAATTACC ATCTCACCTA CACCTGCAAG AAAGGAACAG TTGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCTTG 
ATTAGATATC ACTGCAAAGA TGGTTTCATT CAACGCCACC TTCCAACTAT CCGTTGCCTA 
GGAAATGGAA GATGGGCTAT GCCTAAAATT ACCTGCCTGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA AGAAATATTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCAAAACATG ATCACCGTTG GAGCCGAAGG TGGCAAGAAT CAAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( XM_015472358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327844.1 Bos taurus versican (VCAN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa91173
Clone ID Related Accession (Same CDS sequence)	XM_015472358.1
Accession Version	XM_015472358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	versican core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104055.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTCC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGACCT GATCGTTGCA AAATGAACCC GTGCCTCAAT 
GGGGGCACCT GTTATCCTAC TGAAACTTCC TACGTGTGCA CCTGCGTGCC AGGATACAGT 
GGTGATCGGT GTGAACTTGA TTTTGATGAA TGTCATTCTA ATCCTTGTCG CAATGGAGCC 
ACATGTATTG ACGGTTTTAA TACCTTCAGG TGTCTCTGCC TCCCGAGCTA CGTTGGTGCG 
CTTTGTGAGC AAGACACGGA GACATGTGAC TATGGCTGGC ACAAATTTCA AGGGCAGTGC 
TACAAGTACT TTGCCCATCG ACGGACGTGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGCGCCCATC TCACCAGTAT CCTGTCTCAC GAGGAACAAA TGTTTGTGAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG TCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA TGAGAACTGG AGGCCGAACC AACCAGACAG CTTCTTTTCT 
ACTGGAGAAG ATTGTGTTGT GATTATATGG CATGAGAATG GCCAGTGGAA TGACGTTCCC 
TGCAATTACC ATCTCACCTA CACCTGCAAG AAAGGAACAG TTGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCTTG 
ATTAGATATC ACTGCAAAGA TGGTTTCATT CAACGCCACC TTCCAACTAT CCGTTGCCTA 
GGAAATGGAA GATGGGCTAT GCCTAAAATT ACCTGCCTGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA AGAAATATTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCAAAACATG ATCACCGTTG GAGCCGAAGG TGGCAAGAAT CAAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327844.1
CDS	254..2224
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKRPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	XM_015472358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472358.1

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTCC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGACCT GATCGTTGCA AAATGAACCC GTGCCTCAAT 
GGGGGCACCT GTTATCCTAC TGAAACTTCC TACGTGTGCA CCTGCGTGCC AGGATACAGT 
GGTGATCGGT GTGAACTTGA TTTTGATGAA TGTCATTCTA ATCCTTGTCG CAATGGAGCC 
ACATGTATTG ACGGTTTTAA TACCTTCAGG TGTCTCTGCC TCCCGAGCTA CGTTGGTGCG 
CTTTGTGAGC AAGACACGGA GACATGTGAC TATGGCTGGC ACAAATTTCA AGGGCAGTGC 
TACAAGTACT TTGCCCATCG ACGGACGTGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGCGCCCATC TCACCAGTAT CCTGTCTCAC GAGGAACAAA TGTTTGTGAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG TCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA TGAGAACTGG AGGCCGAACC AACCAGACAG CTTCTTTTCT 
ACTGGAGAAG ATTGTGTTGT GATTATATGG CATGAGAATG GCCAGTGGAA TGACGTTCCC 
TGCAATTACC ATCTCACCTA CACCTGCAAG AAAGGAACAG TTGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCTTG 
ATTAGATATC ACTGCAAAGA TGGTTTCATT CAACGCCACC TTCCAACTAT CCGTTGCCTA 
GGAAATGGAA GATGGGCTAT GCCTAAAATT ACCTGCCTGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA AGAAATATTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCAAAACATG ATCACCGTTG GAGCCGAAGG TGGCAAGAAT CAAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( XM_005209720.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209777.2 Bos taurus versican (VCAN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235782
Clone ID Related Accession (Same CDS sequence)	XM_005209720.4
Accession Version	XM_005209720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	versican core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACCTAAA CAGAATATAT CAGAGGCTAC AACCATCGAA 
CTGAATATGC TTGCAGAAAC TGTCTCACCC ACTTTATTGG AAGAACTACA AATGGGACTG 
GACAGAATGA CACCAATCGT CCCTTTAATC ACTGAATTAC CTGTCATTAC AACAAAGGTC 
CCTCCTATAG GAAATATTGT CAATTTTGAA CAGAAATCCA CAGTTCAGCC TCTAACTAGT 
ACCCACAGAT CAGCCACAGA ATCACTGCCA CCTGATGGAA GTATGAAGAA GCCTTGGGAT 
ATGGACTATT ACTCACCTTC CGCTTCAGGA CCTCTTGGAG AGCCAGACGT ATCTGAAATT 
AAGGAAGAAG TGCCCCAAAG TACAACTGTC GTTTCCCATC ATGCTCCTGA TTCATGGGAT 
GGTGTGAAGG AAGATCTGCA AATAAAAGAT TCAGTTACAC AAATTGAACA AATAGAAGTG 
GGTCCCTTGG TAACATCTAT GGAAATCTCA AAGCACATTC CTTCTAAGGA ATTCACTGTA 
ACTGTGACAC CATTTGTATC TACAACGATG ACCCTGGAAT CAAAAACTGA AAAGAAAGCA 
ATAAGCACAG TCTCTGAATC AGTGACCACA AGCCACTATG GATTCACCTT GAGAGAAGGT 
GATGGTGAAG ATAGAATATC TACAGTTAGA TCGGGTCAGA GTACCTCGAT CTTTAGCCAA 
ATACCTGAGG TCATTACAGT GTCAAAGACT TCAGAAGACA CCACCCGCGG TCAACTAGAG 
GATGTGGAGT CAGTCTCCGC ATCAACGATT GTCTCACCTG ACAGTGATGG GTCGCCCATG 
GACCACAGGC AAGAGAAACA GACTCATGGC AGGATAACTG AAGGTTTTCT TGGCCAGTAT 
GTGTCCACCA CACCTTTCCC ATCACAGCAT CATACAGAAG TAGAATTGTT TCCTTATTCT 
GGTGATAAAA GATTAGTCGA GGGGACATCC ACAGTTATTT CTCCCACTCC ACGAACAGGA 
AGAGAAAGAA CAGAAACTCT AAGACCAGCG ATGAGAACAG TTACTTACAC CAATGATGAA 
ATACAGGAAA AGATCACCAA AGATTCATCT ATAGAAAAAA TCGAGGAAGA AGGCTTCTCT 
GGAATGAAGT TCCCTACAGC TTCCCCAGAG CAAATTCATC ATACCAAGTA TTCAGTGGGA 
ATGACCAAAT CTTTTGAATC CCCAGCACTG ATGACAACGA CAAAGCCAGG CGTGACACCA 
ACAGAGGCAA CAGATGTGGA GGAAGACTTT ACAACTCCAA GTGGATTAGA AACAGATGGC 
TATCAAGATA CCACAGAGTA TGAGGAGGGT ATTACAACCG TGCATTTGAT CCAGAGCACT 
TTAAATGTGG AGGTGGTTAC TGTATCAAAA TGGTCATTGG ATGAAGATAA TACAACATCA 
AAACCTTTAG GGTCCACAGA ACATGTAGGC TCTCCAAAAT TGCCCCCTGC CTTAATCACC 
ACCACGGGAG TGAGTGGTAA GGATAAAGAA ATGCCAAGTC TTACTGAAGA TGGAAGAGAT 
GAATTCACTC GTATCCCAGG TAGTACTCAA AGGCCACTAG AGGAGTTTAC TGAGGAAGAC 
ACAACAGACC ATGAAAAATT CACAGTTAGA TTTCAGCCAA CTACATCAAT TGCTACCACA 
GAAAAGTCTA CTTTGAGAGA TTCTATAACT GAAGAAAGAG TTCCACCTTT TACAAGCACA 
GAAGTCCGAG TTACTCATGC AACCATAGAG GGAAGTGCTT TGGATGAAGG AGAAGATGTG 
GATGTCTCTA AGCCTTTGTC TACTGTCCCT CAATTTGCAC ACCCTTCAGA TGTGGAAGGG 
TCAACCTTTG TTAATTATAG TAGCACCCAG GAGCCTACTA CTTATGTAGA CACTTCCCAT 
ACCATTCCTC TTCCTGTAAT CCCCAAGACA GAATGGGGAG TGTTGGTACC TTCCATTCCA 
TCAGAAGGTG AAGTTCTAGG CGAACCTTCT CAAGACATAC GTGTCATTAA TCAGACTCAC 
TTTGAAGCTT CTATGTATCC TGAGACTGTA AGAACAACAA CAGAAATTAC ACAAGAAGCA 
ACGCGAGAAG ATTTTCTTTG GAAAGAACAG ACTCCAGAGA AACCAGTTTC TCCTCCCAGT 
TCTACAACTG ACACAGCCAA GGAGACAACA CCACCTTTGG ATGAACAAGA GAGCGATGGG 
TCAGCATATA CTGTCTTTGA AGACAGATCA GTGATGGGTT CTGACAGAGT CTCAGTTTTA 
GTAACAACTC CAATTGGAAA ATTTGAGCAG CATACATCTT TTCCACCTGG TGCTGTCACC 
AAAGCAAAAA CAGATGAAGT GGTAACGCTA ACACCAACCA CAGGGTCAAA GGTAACTTTC 
AGTCCATGGC CTAAACAAAA ATATGAAACA GAAGGTACCA GTCCAAGGGG ATTTGTATCT 
CCTTTTAGCA TTGGTGTTAC CCAGCTTATA GAAGAGACCA CGACTGAGAA AAGAGAGAAA 
ACATCCCTGG ATTATATTGA TTTAGGCTCA GGATTATTCG AAAAGCCCAA GGCCACAGAA 
CTCCCAGAAT TTTCAACAGT TAAAGCCACA GTTCCAAGTG ACACTGCTGC CTTCAGTTCA 
GCAGATAGAC TTCACACACC TTCAGCCTCC ACTGAGAAGC CACCTCTCAT TGACAGAGAA 
CCTGATGAAG AAACAACCAG TGACATGGTA ATCATTGGAG AATCAACATC TCGTGTTCCT 
CCCACTACCC TTGAGGATAT TGTAGCCAAG GAAACAGAAA CAGATATTGA TAGAGAATAT 
TTCACAACTT CAAGTACTTC TACTACACAG CCAACGAGAC CTCCCACTGT GGAAGGCAAA 
GAAGCCTTCG GACCACAGGC ATTTTCTACT CCAGAACCCC CAGCAGGGAC AAAATTCCAC 
CCTGACATAA ATGTTTATAT TATTGAGGTC AGAGAAAACA AGACAGGACC TGATCGTTGC 
AAAATGAACC CGTGCCTCAA TGGGGGCACC TGTTATCCTA CTGAAACTTC CTACGTGTGC 
ACCTGCGTGC CAGGATACAG TGGTGATCGG TGTGAACTTG ATTTTGATGA ATGTCATTCT 
AATCCTTGTC GCAATGGAGC CACATGTATT GACGGTTTTA ATACCTTCAG GTGTCTCTGC 
CTCCCGAGCT ACGTTGGTGC GCTTTGTGAG CAAGACACGG AGACATGTGA CTATGGCTGG 
CACAAATTTC AAGGGCAGTG CTACAAGTAC TTTGCCCATC GACGGACGTG GGATGCAGCT 
GAACGGGAAT GCCGTCTGCA GGGCGCCCAT CTCACCAGTA TCCTGTCTCA CGAGGAACAA 
ATGTTTGTGA ATCGTGTGGG CCATGATTAT CAGTGGATAG GTCTCAATGA CAAGATGTTT 
GAGCATGACT TCCGTTGGAC TGATGGCAGC ACACTGCAAT ATGAGAACTG GAGGCCGAAC 
CAACCAGACA GCTTCTTTTC TACTGGAGAA GATTGTGTTG TGATTATATG GCATGAGAAT 
GGCCAGTGGA ATGACGTTCC CTGCAATTAC CATCTCACCT ACACCTGCAA GAAAGGAACA 
GTTGCTTGCG GCCAGCCCCC TGTTGTAGAA AATGCCAAGA CCTTTGGAAA GATGAAACCT 
CGTTATGAAA TCAACTCCTT GATTAGATAT CACTGCAAAG ATGGTTTCAT TCAACGCCAC 
CTTCCAACTA TCCGTTGCCT AGGAAATGGA AGATGGGCTA TGCCTAAAAT TACCTGCCTG 
AACCCATCTG CATACCAAAG GACTTATTCT AAGAAATATT TTAAAAATTC CTCATCAGCA 
AAGGACAATT CAATAAATAC ATCAAAACAT GATCACCGTT GGAGCCGAAG GTGGCAAGAA 
TCAAGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209777.2
CDS	561..5492
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPTPDSRFDAYCFKPKQNISEATTIELNMLAETVSPTLLEELQMGLDRMTPIVPLITELPVITTKVPPIGNIVNFEQKSTVQPLTSTHRSATESLPPDGSMKKPWDMDYYSPSASGPLGEPDVSEIKEEVPQSTTVVSHHAPDSWDGVKEDLQIKDSVTQIEQIEVGPLVTSMEISKHIPSKEFTVTVTPFVSTTMTLESKTEKKAISTVSESVTTSHYGFTLREGDGEDRISTVRSGQSTSIFSQIPEVITVSKTSEDTTRGQLEDVESVSASTIVSPDSDGSPMDHRQEKQTHGRITEGFLGQYVSTTPFPSQHHTEVELFPYSGDKRLVEGTSTVISPTPRTGRERTETLRPAMRTVTYTNDEIQEKITKDSSIEKIEEEGFSGMKFPTASPEQIHHTKYSVGMTKSFESPALMTTTKPGVTPTEATDVEEDFTTPSGLETDGYQDTTEYEEGITTVHLIQSTLNVEVVTVSKWSLDEDNTTSKPLGSTEHVGSPKLPPALITTTGVSGKDKEMPSLTEDGRDEFTRIPGSTQRPLEEFTEEDTTDHEKFTVRFQPTTSIATTEKSTLRDSITEERVPPFTSTEVRVTHATIEGSALDEGEDVDVSKPLSTVPQFAHPSDVEGSTFVNYSSTQEPTTYVDTSHTIPLPVIPKTEWGVLVPSIPSEGEVLGEPSQDIRVINQTHFEASMYPETVRTTTEITQEATREDFLWKEQTPEKPVSPPSSTTDTAKETTPPLDEQESDGSAYTVFEDRSVMGSDRVSVLVTTPIGKFEQHTSFPPGAVTKAKTDEVVTLTPTTGSKVTFSPWPKQKYETEGTSPRGFVSPFSIGVTQLIEETTTEKREKTSLDYIDLGSGLFEKPKATELPEFSTVKATVPSDTAAFSSADRLHTPSASTEKPPLIDREPDEETTSDMVIIGESTSRVPPTTLEDIVAKETETDIDREYFTTSSTSTTQPTRPPTVEGKEAFGPQAFSTPEPPAGTKFHPDINVYIIEVRENKTGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	XM_005209720.4
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209720.4

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACCTAAA CAGAATATAT CAGAGGCTAC AACCATCGAA 
CTGAATATGC TTGCAGAAAC TGTCTCACCC ACTTTATTGG AAGAACTACA AATGGGACTG 
GACAGAATGA CACCAATCGT CCCTTTAATC ACTGAATTAC CTGTCATTAC AACAAAGGTC 
CCTCCTATAG GAAATATTGT CAATTTTGAA CAGAAATCCA CAGTTCAGCC TCTAACTAGT 
ACCCACAGAT CAGCCACAGA ATCACTGCCA CCTGATGGAA GTATGAAGAA GCCTTGGGAT 
ATGGACTATT ACTCACCTTC CGCTTCAGGA CCTCTTGGAG AGCCAGACGT ATCTGAAATT 
AAGGAAGAAG TGCCCCAAAG TACAACTGTC GTTTCCCATC ATGCTCCTGA TTCATGGGAT 
GGTGTGAAGG AAGATCTGCA AATAAAAGAT TCAGTTACAC AAATTGAACA AATAGAAGTG 
GGTCCCTTGG TAACATCTAT GGAAATCTCA AAGCACATTC CTTCTAAGGA ATTCACTGTA 
ACTGTGACAC CATTTGTATC TACAACGATG ACCCTGGAAT CAAAAACTGA AAAGAAAGCA 
ATAAGCACAG TCTCTGAATC AGTGACCACA AGCCACTATG GATTCACCTT GAGAGAAGGT 
GATGGTGAAG ATAGAATATC TACAGTTAGA TCGGGTCAGA GTACCTCGAT CTTTAGCCAA 
ATACCTGAGG TCATTACAGT GTCAAAGACT TCAGAAGACA CCACCCGCGG TCAACTAGAG 
GATGTGGAGT CAGTCTCCGC ATCAACGATT GTCTCACCTG ACAGTGATGG GTCGCCCATG 
GACCACAGGC AAGAGAAACA GACTCATGGC AGGATAACTG AAGGTTTTCT TGGCCAGTAT 
GTGTCCACCA CACCTTTCCC ATCACAGCAT CATACAGAAG TAGAATTGTT TCCTTATTCT 
GGTGATAAAA GATTAGTCGA GGGGACATCC ACAGTTATTT CTCCCACTCC ACGAACAGGA 
AGAGAAAGAA CAGAAACTCT AAGACCAGCG ATGAGAACAG TTACTTACAC CAATGATGAA 
ATACAGGAAA AGATCACCAA AGATTCATCT ATAGAAAAAA TCGAGGAAGA AGGCTTCTCT 
GGAATGAAGT TCCCTACAGC TTCCCCAGAG CAAATTCATC ATACCAAGTA TTCAGTGGGA 
ATGACCAAAT CTTTTGAATC CCCAGCACTG ATGACAACGA CAAAGCCAGG CGTGACACCA 
ACAGAGGCAA CAGATGTGGA GGAAGACTTT ACAACTCCAA GTGGATTAGA AACAGATGGC 
TATCAAGATA CCACAGAGTA TGAGGAGGGT ATTACAACCG TGCATTTGAT CCAGAGCACT 
TTAAATGTGG AGGTGGTTAC TGTATCAAAA TGGTCATTGG ATGAAGATAA TACAACATCA 
AAACCTTTAG GGTCCACAGA ACATGTAGGC TCTCCAAAAT TGCCCCCTGC CTTAATCACC 
ACCACGGGAG TGAGTGGTAA GGATAAAGAA ATGCCAAGTC TTACTGAAGA TGGAAGAGAT 
GAATTCACTC GTATCCCAGG TAGTACTCAA AGGCCACTAG AGGAGTTTAC TGAGGAAGAC 
ACAACAGACC ATGAAAAATT CACAGTTAGA TTTCAGCCAA CTACATCAAT TGCTACCACA 
GAAAAGTCTA CTTTGAGAGA TTCTATAACT GAAGAAAGAG TTCCACCTTT TACAAGCACA 
GAAGTCCGAG TTACTCATGC AACCATAGAG GGAAGTGCTT TGGATGAAGG AGAAGATGTG 
GATGTCTCTA AGCCTTTGTC TACTGTCCCT CAATTTGCAC ACCCTTCAGA TGTGGAAGGG 
TCAACCTTTG TTAATTATAG TAGCACCCAG GAGCCTACTA CTTATGTAGA CACTTCCCAT 
ACCATTCCTC TTCCTGTAAT CCCCAAGACA GAATGGGGAG TGTTGGTACC TTCCATTCCA 
TCAGAAGGTG AAGTTCTAGG CGAACCTTCT CAAGACATAC GTGTCATTAA TCAGACTCAC 
TTTGAAGCTT CTATGTATCC TGAGACTGTA AGAACAACAA CAGAAATTAC ACAAGAAGCA 
ACGCGAGAAG ATTTTCTTTG GAAAGAACAG ACTCCAGAGA AACCAGTTTC TCCTCCCAGT 
TCTACAACTG ACACAGCCAA GGAGACAACA CCACCTTTGG ATGAACAAGA GAGCGATGGG 
TCAGCATATA CTGTCTTTGA AGACAGATCA GTGATGGGTT CTGACAGAGT CTCAGTTTTA 
GTAACAACTC CAATTGGAAA ATTTGAGCAG CATACATCTT TTCCACCTGG TGCTGTCACC 
AAAGCAAAAA CAGATGAAGT GGTAACGCTA ACACCAACCA CAGGGTCAAA GGTAACTTTC 
AGTCCATGGC CTAAACAAAA ATATGAAACA GAAGGTACCA GTCCAAGGGG ATTTGTATCT 
CCTTTTAGCA TTGGTGTTAC CCAGCTTATA GAAGAGACCA CGACTGAGAA AAGAGAGAAA 
ACATCCCTGG ATTATATTGA TTTAGGCTCA GGATTATTCG AAAAGCCCAA GGCCACAGAA 
CTCCCAGAAT TTTCAACAGT TAAAGCCACA GTTCCAAGTG ACACTGCTGC CTTCAGTTCA 
GCAGATAGAC TTCACACACC TTCAGCCTCC ACTGAGAAGC CACCTCTCAT TGACAGAGAA 
CCTGATGAAG AAACAACCAG TGACATGGTA ATCATTGGAG AATCAACATC TCGTGTTCCT 
CCCACTACCC TTGAGGATAT TGTAGCCAAG GAAACAGAAA CAGATATTGA TAGAGAATAT 
TTCACAACTT CAAGTACTTC TACTACACAG CCAACGAGAC CTCCCACTGT GGAAGGCAAA 
GAAGCCTTCG GACCACAGGC ATTTTCTACT CCAGAACCCC CAGCAGGGAC AAAATTCCAC 
CCTGACATAA ATGTTTATAT TATTGAGGTC AGAGAAAACA AGACAGGACC TGATCGTTGC 
AAAATGAACC CGTGCCTCAA TGGGGGCACC TGTTATCCTA CTGAAACTTC CTACGTGTGC 
ACCTGCGTGC CAGGATACAG TGGTGATCGG TGTGAACTTG ATTTTGATGA ATGTCATTCT 
AATCCTTGTC GCAATGGAGC CACATGTATT GACGGTTTTA ATACCTTCAG GTGTCTCTGC 
CTCCCGAGCT ACGTTGGTGC GCTTTGTGAG CAAGACACGG AGACATGTGA CTATGGCTGG 
CACAAATTTC AAGGGCAGTG CTACAAGTAC TTTGCCCATC GACGGACGTG GGATGCAGCT 
GAACGGGAAT GCCGTCTGCA GGGCGCCCAT CTCACCAGTA TCCTGTCTCA CGAGGAACAA 
ATGTTTGTGA ATCGTGTGGG CCATGATTAT CAGTGGATAG GTCTCAATGA CAAGATGTTT 
GAGCATGACT TCCGTTGGAC TGATGGCAGC ACACTGCAAT ATGAGAACTG GAGGCCGAAC 
CAACCAGACA GCTTCTTTTC TACTGGAGAA GATTGTGTTG TGATTATATG GCATGAGAAT 
GGCCAGTGGA ATGACGTTCC CTGCAATTAC CATCTCACCT ACACCTGCAA GAAAGGAACA 
GTTGCTTGCG GCCAGCCCCC TGTTGTAGAA AATGCCAAGA CCTTTGGAAA GATGAAACCT 
CGTTATGAAA TCAACTCCTT GATTAGATAT CACTGCAAAG ATGGTTTCAT TCAACGCCAC 
CTTCCAACTA TCCGTTGCCT AGGAAATGGA AGATGGGCTA TGCCTAAAAT TACCTGCCTG 
AACCCATCTG CATACCAAAG GACTTATTCT AAGAAATATT TTAAAAATTC CTCATCAGCA 
AAGGACAATT CAATAAATAC ATCAAAACAT GATCACCGTT GGAGCCGAAG GTGGCAAGAA 
TCAAGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( XM_005209720.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209777.1 Bos taurus versican (VCAN), transcript variant X2, mRNA.

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CloneID	OBa05606
Clone ID Related Accession (Same CDS sequence)	XM_005209720.3
Accession Version	XM_005209720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	versican core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104055.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTCC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACCTAAA CAGAATATAT CAGAGGCTAC AACCATCGAA 
CTGAATATGC TTGCAGAAAC TGTCTCACCC ACTTTATTGG AAGAACTACA AATGGGACTG 
GACAGAATGA CACCAATCGT CCCTTTAATC ACTGAATTAC CTGTCATTAC AACAAAGGTC 
CCTCCTATAG GAAATATTGT CAATTTTGAA CAGAAATCCA CAGTTCAGCC TCTAACTAGT 
ACCCACAGAT CAGCCACAGA ATCACTGCCA CCTGATGGAA GTATGAAGAA GCCTTGGGAT 
ATGGACTATT ACTCACCTTC CGCTTCAGGA CCTCTTGGAG AGCCAGACGT ATCTGAAATT 
AAGGAAGAAG TGCCCCAAAG TACAACTGTC GTTTCCCATC ATGCTCCTGA TTCATGGGAT 
GGTGTGAAGG AAGATCTGCA AATAAAAGAT TCAGTTACAC AAATTGAACA AATAGAAGTG 
GGTCCCTTGG TAACATCTAT GGAAATCTCA AAGCACATTC CTTCTAAGGA ATTCACTGTA 
ACTGTGACAC CATTTGTATC TACAACGATG ACCCTGGAAT CAAAAACTGA AAAGAAAGCA 
ATAAGCACAG TCTCTGAATC AGTGACCACA AGCCACTATG GATTCACCTT GAGAGAAGGT 
GATGGTGAAG ATAGAATATC TACAGTTAGA TCGGGTCAGA GTACCTCGAT CTTTAGCCAA 
ATACCTGAGG TCATTACAGT GTCAAAGACT TCAGAAGACA CCACCCGCGG TCAACTAGAG 
GATGTGGAGT CAGTCTCCGC ATCAACGATT GTCTCACCTG ACAGTGATGG GTCGCCCATG 
GACCACAGGC AAGAGAAACA GACTCATGGC AGGATAACTG AAGGTTTTCT TGGCCAGTAT 
GTGTCCACCA CACCTTTCCC ATCACAGCAT CATACAGAAG TAGAATTGTT TCCTTATTCT 
GGTGATAAAA GATTAGTCGA GGGGACATCC ACAGTTATTT CTCCCACTCC ACGAACAGGA 
AGAGAAAGAA CAGAAACTCT AAGACCAGCG ATGAGAACAG TTACTTACAC CAATGATGAA 
ATACAGGAAA AGATCACCAA AGATTCATCT ATAGAAAAAA TCGAGGAAGA AGGCTTCTCT 
GGAATGAAGT TCCCTACAGC TTCCCCAGAG CAAATTCATC ATACCAAGTA TTCAGTGGGA 
ATGACCAAAT CTTTTGAATC CCCAGCACTG ATGACAACGA CAAAGCCAGG CGTGACACCA 
ACAGAGGCAA CAGATGTGGA GGAAGACTTT ACAACTCCAA GTGGATTAGA AACAGATGGC 
TATCAAGATA CCACAGAGTA TGAGGAGGGT ATTACAACCG TGCATTTGAT CCAGAGCACT 
TTAAATGTGG AGGTGGTTAC TGTATCAAAA TGGTCATTGG ATGAAGATAA TACAACATCA 
AAACCTTTAG GGTCCACAGA ACATGTAGGC TCTCCAAAAT TGCCCCCTGC CTTAATCACC 
ACCACGGGAG TGAGTGGTAA GGATAAAGAA ATGCCAAGTC TTACTGAAGA TGGAAGAGAT 
GAATTCACTC GTATCCCAGG TAGTACTCAA AGGCCACTAG AGGAGTTTAC TGAGGAAGAC 
ACAACAGACC ATGAAAAATT CACAGTTAGA TTTCAGCCAA CTACATCAAT TGCTACCACA 
GAAAAGTCTA CTTTGAGAGA TTCTATAACT GAAGAAAGAG TTCCACCTTT TACAAGCACA 
GAAGTCCGAG TTACTCATGC AACCATAGAG GGAAGTGCTT TGGATGAAGG AGAAGATGTG 
GATGTCTCTA AGCCTTTGTC TACTGTCCCT CAATTTGCAC ACCCTTCAGA TGTGGAAGGG 
TCAACCTTTG TTAATTATAG TAGCACCCAG GAGCCTACTA CTTATGTAGA CACTTCCCAT 
ACCATTCCTC TTCCTGTAAT CCCCAAGACA GAATGGGGAG TGTTGGTACC TTCCATTCCA 
TCAGAAGGTG AAGTTCTAGG CGAACCTTCT CAAGACATAC GTGTCATTAA TCAGACTCAC 
TTTGAAGCTT CTATGTATCC TGAGACTGTA AGAACAACAA CAGAAATTAC ACAAGAAGCA 
ACGCGAGAAG ATTTTCTTTG GAAAGAACAG ACTCCAGAGA AACCAGTTTC TCCTCCCAGT 
TCTACAACTG ACACAGCCAA GGAGACAACA CCACCTTTGG ATGAACAAGA GAGCGATGGG 
TCAGCATATA CTGTCTTTGA AGACAGATCA GTGATGGGTT CTGACAGAGT CTCAGTTTTA 
GTAACAACTC CAATTGGAAA ATTTGAGCAG CATACATCTT TTCCACCTGG TGCTGTCACC 
AAAGCAAAAA CAGATGAAGT GGTAACGCTA ACACCAACCA CAGGGTCAAA GGTAACTTTC 
AGTCCATGGC CTAAACAAAA ATATGAAACA GAAGGTACCA GTCCAAGGGG ATTTGTATCT 
CCTTTTAGCA TTGGTGTTAC CCAGCTTATA GAAGAGACCA CGACTGAGAA AAGAGAGAAA 
ACATCCCTGG ATTATATTGA TTTAGGCTCA GGATTATTCG AAAAGCCCAA GGCCACAGAA 
CTCCCAGAAT TTTCAACAGT TAAAGCCACA GTTCCAAGTG ACACTGCTGC CTTCAGTTCA 
GCAGATAGAC TTCACACACC TTCAGCCTCC ACTGAGAAGC CACCTCTCAT TGACAGAGAA 
CCTGATGAAG AAACAACCAG TGACATGGTA ATCATTGGAG AATCAACATC TCGTGTTCCT 
CCCACTACCC TTGAGGATAT TGTAGCCAAG GAAACAGAAA CAGATATTGA TAGAGAATAT 
TTCACAACTT CAAGTACTTC TACTACACAG CCAACGAGAC CTCCCACTGT GGAAGGCAAA 
GAAGCCTTCG GACCACAGGC ATTTTCTACT CCAGAACCCC CAGCAGGGAC AAAATTCCAC 
CCTGACATAA ATGTTTATAT TATTGAGGTC AGAGAAAACA AGACAGGACC TGATCGTTGC 
AAAATGAACC CGTGCCTCAA TGGGGGCACC TGTTATCCTA CTGAAACTTC CTACGTGTGC 
ACCTGCGTGC CAGGATACAG TGGTGATCGG TGTGAACTTG ATTTTGATGA ATGTCATTCT 
AATCCTTGTC GCAATGGAGC CACATGTATT GACGGTTTTA ATACCTTCAG GTGTCTCTGC 
CTCCCGAGCT ACGTTGGTGC GCTTTGTGAG CAAGACACGG AGACATGTGA CTATGGCTGG 
CACAAATTTC AAGGGCAGTG CTACAAGTAC TTTGCCCATC GACGGACGTG GGATGCAGCT 
GAACGGGAAT GCCGTCTGCA GGGCGCCCAT CTCACCAGTA TCCTGTCTCA CGAGGAACAA 
ATGTTTGTGA ATCGTGTGGG CCATGATTAT CAGTGGATAG GTCTCAATGA CAAGATGTTT 
GAGCATGACT TCCGTTGGAC TGATGGCAGC ACACTGCAAT ATGAGAACTG GAGGCCGAAC 
CAACCAGACA GCTTCTTTTC TACTGGAGAA GATTGTGTTG TGATTATATG GCATGAGAAT 
GGCCAGTGGA ATGACGTTCC CTGCAATTAC CATCTCACCT ACACCTGCAA GAAAGGAACA 
GTTGCTTGCG GCCAGCCCCC TGTTGTAGAA AATGCCAAGA CCTTTGGAAA GATGAAACCT 
CGTTATGAAA TCAACTCCTT GATTAGATAT CACTGCAAAG ATGGTTTCAT TCAACGCCAC 
CTTCCAACTA TCCGTTGCCT AGGAAATGGA AGATGGGCTA TGCCTAAAAT TACCTGCCTG 
AACCCATCTG CATACCAAAG GACTTATTCT AAGAAATATT TTAAAAATTC CTCATCAGCA 
AAGGACAATT CAATAAATAC ATCAAAACAT GATCACCGTT GGAGCCGAAG GTGGCAAGAA 
TCAAGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209777.1
CDS	376..5307
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKQNISEATTIELNMLAETVSPTLLEELQMGLDRMTPIVPLITELPVITTKVPPIGNIVNFEQKSTVQPLTSTHRSATESLPPDGSMKKPWDMDYYSPSASGPLGEPDVSEIKEEVPQSTTVVSHHAPDSWDGVKEDLQIKDSVTQIEQIEVGPLVTSMEISKHIPSKEFTVTVTPFVSTTMTLESKTEKKAISTVSESVTTSHYGFTLREGDGEDRISTVRSGQSTSIFSQIPEVITVSKTSEDTTRGQLEDVESVSASTIVSPDSDGSPMDHRQEKQTHGRITEGFLGQYVSTTPFPSQHHTEVELFPYSGDKRLVEGTSTVISPTPRTGRERTETLRPAMRTVTYTNDEIQEKITKDSSIEKIEEEGFSGMKFPTASPEQIHHTKYSVGMTKSFESPALMTTTKPGVTPTEATDVEEDFTTPSGLETDGYQDTTEYEEGITTVHLIQSTLNVEVVTVSKWSLDEDNTTSKPLGSTEHVGSPKLPPALITTTGVSGKDKEMPSLTEDGRDEFTRIPGSTQRPLEEFTEEDTTDHEKFTVRFQPTTSIATTEKSTLRDSITEERVPPFTSTEVRVTHATIEGSALDEGEDVDVSKPLSTVPQFAHPSDVEGSTFVNYSSTQEPTTYVDTSHTIPLPVIPKTEWGVLVPSIPSEGEVLGEPSQDIRVINQTHFEASMYPETVRTTTEITQEATREDFLWKEQTPEKPVSPPSSTTDTAKETTPPLDEQESDGSAYTVFEDRSVMGSDRVSVLVTTPIGKFEQHTSFPPGAVTKAKTDEVVTLTPTTGSKVTFSPWPKQKYETEGTSPRGFVSPFSIGVTQLIEETTTEKREKTSLDYIDLGSGLFEKPKATELPEFSTVKATVPSDTAAFSSADRLHTPSASTEKPPLIDREPDEETTSDMVIIGESTSRVPPTTLEDIVAKETETDIDREYFTTSSTSTTQPTRPPTVEGKEAFGPQAFSTPEPPAGTKFHPDINVYIIEVRENKTGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	XM_005209720.3
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209720.3

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTCC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACCTAAA CAGAATATAT CAGAGGCTAC AACCATCGAA 
CTGAATATGC TTGCAGAAAC TGTCTCACCC ACTTTATTGG AAGAACTACA AATGGGACTG 
GACAGAATGA CACCAATCGT CCCTTTAATC ACTGAATTAC CTGTCATTAC AACAAAGGTC 
CCTCCTATAG GAAATATTGT CAATTTTGAA CAGAAATCCA CAGTTCAGCC TCTAACTAGT 
ACCCACAGAT CAGCCACAGA ATCACTGCCA CCTGATGGAA GTATGAAGAA GCCTTGGGAT 
ATGGACTATT ACTCACCTTC CGCTTCAGGA CCTCTTGGAG AGCCAGACGT ATCTGAAATT 
AAGGAAGAAG TGCCCCAAAG TACAACTGTC GTTTCCCATC ATGCTCCTGA TTCATGGGAT 
GGTGTGAAGG AAGATCTGCA AATAAAAGAT TCAGTTACAC AAATTGAACA AATAGAAGTG 
GGTCCCTTGG TAACATCTAT GGAAATCTCA AAGCACATTC CTTCTAAGGA ATTCACTGTA 
ACTGTGACAC CATTTGTATC TACAACGATG ACCCTGGAAT CAAAAACTGA AAAGAAAGCA 
ATAAGCACAG TCTCTGAATC AGTGACCACA AGCCACTATG GATTCACCTT GAGAGAAGGT 
GATGGTGAAG ATAGAATATC TACAGTTAGA TCGGGTCAGA GTACCTCGAT CTTTAGCCAA 
ATACCTGAGG TCATTACAGT GTCAAAGACT TCAGAAGACA CCACCCGCGG TCAACTAGAG 
GATGTGGAGT CAGTCTCCGC ATCAACGATT GTCTCACCTG ACAGTGATGG GTCGCCCATG 
GACCACAGGC AAGAGAAACA GACTCATGGC AGGATAACTG AAGGTTTTCT TGGCCAGTAT 
GTGTCCACCA CACCTTTCCC ATCACAGCAT CATACAGAAG TAGAATTGTT TCCTTATTCT 
GGTGATAAAA GATTAGTCGA GGGGACATCC ACAGTTATTT CTCCCACTCC ACGAACAGGA 
AGAGAAAGAA CAGAAACTCT AAGACCAGCG ATGAGAACAG TTACTTACAC CAATGATGAA 
ATACAGGAAA AGATCACCAA AGATTCATCT ATAGAAAAAA TCGAGGAAGA AGGCTTCTCT 
GGAATGAAGT TCCCTACAGC TTCCCCAGAG CAAATTCATC ATACCAAGTA TTCAGTGGGA 
ATGACCAAAT CTTTTGAATC CCCAGCACTG ATGACAACGA CAAAGCCAGG CGTGACACCA 
ACAGAGGCAA CAGATGTGGA GGAAGACTTT ACAACTCCAA GTGGATTAGA AACAGATGGC 
TATCAAGATA CCACAGAGTA TGAGGAGGGT ATTACAACCG TGCATTTGAT CCAGAGCACT 
TTAAATGTGG AGGTGGTTAC TGTATCAAAA TGGTCATTGG ATGAAGATAA TACAACATCA 
AAACCTTTAG GGTCCACAGA ACATGTAGGC TCTCCAAAAT TGCCCCCTGC CTTAATCACC 
ACCACGGGAG TGAGTGGTAA GGATAAAGAA ATGCCAAGTC TTACTGAAGA TGGAAGAGAT 
GAATTCACTC GTATCCCAGG TAGTACTCAA AGGCCACTAG AGGAGTTTAC TGAGGAAGAC 
ACAACAGACC ATGAAAAATT CACAGTTAGA TTTCAGCCAA CTACATCAAT TGCTACCACA 
GAAAAGTCTA CTTTGAGAGA TTCTATAACT GAAGAAAGAG TTCCACCTTT TACAAGCACA 
GAAGTCCGAG TTACTCATGC AACCATAGAG GGAAGTGCTT TGGATGAAGG AGAAGATGTG 
GATGTCTCTA AGCCTTTGTC TACTGTCCCT CAATTTGCAC ACCCTTCAGA TGTGGAAGGG 
TCAACCTTTG TTAATTATAG TAGCACCCAG GAGCCTACTA CTTATGTAGA CACTTCCCAT 
ACCATTCCTC TTCCTGTAAT CCCCAAGACA GAATGGGGAG TGTTGGTACC TTCCATTCCA 
TCAGAAGGTG AAGTTCTAGG CGAACCTTCT CAAGACATAC GTGTCATTAA TCAGACTCAC 
TTTGAAGCTT CTATGTATCC TGAGACTGTA AGAACAACAA CAGAAATTAC ACAAGAAGCA 
ACGCGAGAAG ATTTTCTTTG GAAAGAACAG ACTCCAGAGA AACCAGTTTC TCCTCCCAGT 
TCTACAACTG ACACAGCCAA GGAGACAACA CCACCTTTGG ATGAACAAGA GAGCGATGGG 
TCAGCATATA CTGTCTTTGA AGACAGATCA GTGATGGGTT CTGACAGAGT CTCAGTTTTA 
GTAACAACTC CAATTGGAAA ATTTGAGCAG CATACATCTT TTCCACCTGG TGCTGTCACC 
AAAGCAAAAA CAGATGAAGT GGTAACGCTA ACACCAACCA CAGGGTCAAA GGTAACTTTC 
AGTCCATGGC CTAAACAAAA ATATGAAACA GAAGGTACCA GTCCAAGGGG ATTTGTATCT 
CCTTTTAGCA TTGGTGTTAC CCAGCTTATA GAAGAGACCA CGACTGAGAA AAGAGAGAAA 
ACATCCCTGG ATTATATTGA TTTAGGCTCA GGATTATTCG AAAAGCCCAA GGCCACAGAA 
CTCCCAGAAT TTTCAACAGT TAAAGCCACA GTTCCAAGTG ACACTGCTGC CTTCAGTTCA 
GCAGATAGAC TTCACACACC TTCAGCCTCC ACTGAGAAGC CACCTCTCAT TGACAGAGAA 
CCTGATGAAG AAACAACCAG TGACATGGTA ATCATTGGAG AATCAACATC TCGTGTTCCT 
CCCACTACCC TTGAGGATAT TGTAGCCAAG GAAACAGAAA CAGATATTGA TAGAGAATAT 
TTCACAACTT CAAGTACTTC TACTACACAG CCAACGAGAC CTCCCACTGT GGAAGGCAAA 
GAAGCCTTCG GACCACAGGC ATTTTCTACT CCAGAACCCC CAGCAGGGAC AAAATTCCAC 
CCTGACATAA ATGTTTATAT TATTGAGGTC AGAGAAAACA AGACAGGACC TGATCGTTGC 
AAAATGAACC CGTGCCTCAA TGGGGGCACC TGTTATCCTA CTGAAACTTC CTACGTGTGC 
ACCTGCGTGC CAGGATACAG TGGTGATCGG TGTGAACTTG ATTTTGATGA ATGTCATTCT 
AATCCTTGTC GCAATGGAGC CACATGTATT GACGGTTTTA ATACCTTCAG GTGTCTCTGC 
CTCCCGAGCT ACGTTGGTGC GCTTTGTGAG CAAGACACGG AGACATGTGA CTATGGCTGG 
CACAAATTTC AAGGGCAGTG CTACAAGTAC TTTGCCCATC GACGGACGTG GGATGCAGCT 
GAACGGGAAT GCCGTCTGCA GGGCGCCCAT CTCACCAGTA TCCTGTCTCA CGAGGAACAA 
ATGTTTGTGA ATCGTGTGGG CCATGATTAT CAGTGGATAG GTCTCAATGA CAAGATGTTT 
GAGCATGACT TCCGTTGGAC TGATGGCAGC ACACTGCAAT ATGAGAACTG GAGGCCGAAC 
CAACCAGACA GCTTCTTTTC TACTGGAGAA GATTGTGTTG TGATTATATG GCATGAGAAT 
GGCCAGTGGA ATGACGTTCC CTGCAATTAC CATCTCACCT ACACCTGCAA GAAAGGAACA 
GTTGCTTGCG GCCAGCCCCC TGTTGTAGAA AATGCCAAGA CCTTTGGAAA GATGAAACCT 
CGTTATGAAA TCAACTCCTT GATTAGATAT CACTGCAAAG ATGGTTTCAT TCAACGCCAC 
CTTCCAACTA TCCGTTGCCT AGGAAATGGA AGATGGGCTA TGCCTAAAAT TACCTGCCTG 
AACCCATCTG CATACCAAAG GACTTATTCT AAGAAATATT TTAAAAATTC CTCATCAGCA 
AAGGACAATT CAATAAATAC ATCAAAACAT GATCACCGTT GGAGCCGAAG GTGGCAAGAA 
TCAAGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( XM_005209719.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209776.2 Bos taurus versican (VCAN), transcript variant X1, mRNA.

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CloneID	OBa235781
Clone ID Related Accession (Same CDS sequence)	XM_005209719.4
Accession Version	XM_005209719.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	versican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209719.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGTCGA ATGAGTGATT TCAGTGTAAG TGGTCATCCA 
ATAGATTCAG AATCTAAAGA AGATGAGCCT TGTAGTGAAG AAACAGATCC AGAGCACGAT 
CTAATTGCTG AAATTTTACC TGAGTTACTT GGTATGTTGC ACAGTGAAGA GGATGAAGAA 
GATGAGGAGT GTGCCAATGC CACTGATGTC ACGACCACCC CATCCGTGCA GTACATAAAT 
GGGAAGCATG TAGTTACCAC TGTTCCCAAG GACCCAGAAG CAGCAGAAGC TAGGCGTGGC 
CAGTTCGAAA GTGTTGCACC TTCTCAAAAT TTTTCAGACA GCAGTGAAAG TGATAGCCAT 
CAGTTTATAA TCACCCATGC TGGACTGCCC ACTGCCATGC AACCTAATGA ATCTAAAGAA 
ACAACGGAGT CCCTTGAAAT TACGTGGAGA CCCGAGATTT ACCCTGAAAC AGCAGAGCCT 
TTCTCAAGTG GCGAGCCTGA TATTTTCCCC ACAGCCTCAA TCCATGAGGG AGAAGCCACT 
GAGGGGCCAG ACTCAATCAC AGAGAAAAAT CCTGAACTTG ATCATCTGGT GCATGAACAT 
GCTGAATCGG TACCTCTGTT TCCTGAAGAG TCTTCAGGAG ATGCTGCCAT TGACCAGGAA 
TCTCAAAAAA TCATCTTTTC AGGGGCTACC GAAGGAACGT TTGGTGAAGA GGCAGAAAAA 
AGTTCTACCA CACATACTCC AAGTATGGTT GCAAGTTCTG TATCAGCACC TGTCTCAGAG 
GATGCATCAT TTATCTTAAC AGGCACTCCA CAGTCTGATG AGCCGTTGTC CACAGTAGAA 
AGCTGGGTGG AAATAACCCC TAGACACACT GTCGAGTTCT CGGGAAGTCC TTCAATTCCA 
ATTCCGGAAG GCTCTGGGGA AGCAGAAGAA GACAAAGATA AAATATTTGC CATGATAACT 
GATTTATCAC AGAGAAATAC TACAGACCCC CGCGTTACTT CAGACACCAG CAAGATCATG 
ATCACGGAGA GCCTTGTGGA TGTTCCTACA ACCACTGTAT ATTCAATTTC TGAACAGGTT 
TCTGCAGTAG TGCCTACCAA ATTTGTAAGG GAGACAGACA CTTATGAGTG GGTTTTCAGT 
CCACCTCTGG AGGAAACAAC AAGGAAAGAG GAGGAGAAGG GAACTACAGG TACAGCTTCT 
ACAGTTGAGG TACATTCCCC TACACAGCGA TTAGATCAGT TTGTTTCACC CTCTGAGTTA 
GAAAGCTCAA GTGAAACTCC ACCTGATGAT TCAGCAGCTG CTACTAGGAA GAGTTTCACG 
TCCCAGATGA CACCCACACA GTCTGAAAGG GAAACGACAA GTTCTACTGT TGTCTTTAAA 
GAAACAGAAG TTTTAGACAA TCTGGCAGCA CAGACCACAG ATCCCAGCCT TAGCAGTCAA 
CCTGGGGTTC TGGAAGTGTC ACCTACTGTC CCAGGGAGCC CCGTCTCTCT CTTTATGGAG 
CAAGGCTCCG GAGAAGCTGC TGTTGACCCA GAAACCACCA CTGTTTCTTC ACTTTCATTA 
AATATAGAGC CTGAAATTCT AGCTGAAGAG GAGGCAGCTG GCACTTGGTC TCCAAATGTG 
GAAACTGTAT TCCCTTTTGA GCCAACCGAA CAAGTCTTAA GTACTGCAGT GGACAGGGAG 
GTTGCTGAAA CTATAAGCCA AACATCTAAG GAAAACTTGG TTTCAGAGAT CTCAGGAGAG 
CCAACTCACA GGGCAGAAAT AAAGGGCTTT TCTACAGATT TTCCTTTGGA GGAAGATTTC 
AGTGGTGACT TCAGAGAATA CTCGACAGTA TCTTATCCCA TAACAAAAGA AGAAATAGTG 
ATGATGGAAG GCTCAGGGGA TGCAGCATTT AAGGATACCC AGATGTTACC ATCTGTAACA 
CCTACCTCGG ACCTCAGTAA CCACACTGCT GACTCGGAAG AACCTGGCAG CACCTTGGTC 
AGCACCTCAG CCTTCCCTTG GGAAGAATTC ACAGCCTCTG CTGAGGGCTC AGGTGAGCCC 
TTGCTCTCAG TAAGCAGCTC TGTTGACCAA GTGTTTCCCA GTGCCGCGGG AAAAGCCTCT 
GGAACAGATT CCCCATTTAT TGACCAACGA TTGGGAGAAG AAGGTGCTAT CAATGAAACT 
GATCAAAGAT CCACCATTTT GCCAACAGCA GAAGCTGAGA GTACTAAAGC TTCAACCGAA 
GAGGGGGAGG TGAAGGAGAA TCACACAGTT TCAATGGACT TTCCCCCAAC TGTGGAGCCA 
GACGAATTAT GGCCTAGACA AGAAGTCAAC CCTGTAAGGC AAGGAAATGG AAGTGAAATA 
GTATCAGAGG AAAAGACTCA AGAGCAAGAA TCTTTTGAAC CCCTTCAAAG CTCTGTTGCA 
CCAGAACAAA CAACTTTTGA TTCACAGACA TTTCCTGAAC CTGGACTCCA AACCACAGGT 
TATTTCACAC TAACGACAAA GAAAACTTAC AGTACTGATG AGAGAATGGA GGAGGAAGTC 
ATTTCCTTAG CTGACGTGTC CACTCCAACT CTAGATTCAA AGGGCTTGGT ATTGTACACT 
ACTCTCCCTG AAGTTACTGA AAAATCCCAT TTTTTCTTAG CTACTGCCTC AGTGACTGAA 
TCAGTACCAG CTGAAAGTGT AATTGCAGGT TCAACCATCA AAGAGGAAGA GAGTATAAAA 
CCCTTTCCCA AAGTTACGAG CCCGATAATT AAAGAGTCAG ATACAGATCT TATATTCTCT 
GGACTGGGAT CTGGGGAAGA AGTTTTGCCT ACTCTAGGAT CAGTGAATTT TACTGAAATA 
GAACAAGTCC TTAGCACATT ATATCCCCTG ACTTCTCAAG TACAAAGTTT AGAAGCCAGC 
ATCTTAAATG ATACAAGTGG AGACTATGAG GGAATGGAAA ATGTAGCAAA TGAAATGAGA 
CCACTCATTT CCAAAACAGA CAGCATTTTT GAAGATGGTG AGACAGCATC CAGCACAACA 
CTACCAGAAA TTTTAAGTGA CGCCAGAACA GAAGGGCCCT TCACGGCACC TCTCACTTTT 
TCCACAGGCC CCGGACAGCC TCAAAATCAG ACGCACCGTA GGGCAGAAGA AATCCAGACC 
AGTAGACCAC AGCCTCTGAC TGATCAAGTC TCTAGCGAGA ATTCTGTGAC AGCAGAAACT 
AAAGAAACAG CAACCCCTGC CACTGATTTT CTGGCTAGAA CATATGATCT TGAAATGGCC 
AAAGGATTTG TTACATCAAC ACCGAAACCA TCTGACCTGT TTTATGAACA TTCTGGAGAA 
GGTTCTGGGG AATTGGATGC CGTTGGTGCA GAAGTCCACG CTTCTGGAAT GACTCAGGCA 
ACTAGGCAAG GAAGCACCAC ATTTGTTTCT GATAGATCCC TGGAAAAACA TCCCAAGGTT 
CCAAGTGTCG AAGCTGTTAC TGTTAATGGA TTCCCGACAG TTTCAATGGT GCTGCCGCTT 
CATCCTGAGC AGAGAGAAGG CTCTCCTGAA GCAACCGGCA CACCGGCAAG CACAGCATCA 
TATGAGAAGG CCACAGAAGG TGCGGCAGAT AGTTTCCAAG ACCATTTCTG GGGATTCAAG 
GATTCCACGC TGAAACCCGA CAAAAGAAAA GCCACTGAAA GTATTATTAT AGATCTGGAC 
AAAGAGGACA AGGATTTAAT ATTGACAATG ACGGAGAGTA CCATCCTTGA AATTATACCT 
GAGCTCACAT CAGATAAAAA TACTATCATA GATATTGATC ACACTAAACC AATATATGAA 
GACATCCTTG GAATGCAGAC GGACTTGGAT CCAGAAGTAC CCTCAGGACC GCCTGACAGT 
AGTGAAGAGA GCACTCAAGT TCAAGAGAAG TATGAGGCAG CTGTTAACCT GTCTTCAACT 
GAGGAAAACT TTGAGGCTTC TGGTGATATT CTGCTGGCTA ATTACACTCA GGCAACACCT 
GAATCGAAGG CTCCTGAAGA CAGAAATCCA TTAGATCACA CAGACTTTAT CTTCACGACT 
GGGATCCCCA TTCTTAGCTC AGAGACAGAA TTAGACGTTT TGCTTCCCAC AGCCACATCT 
CTGCCCATTC CTAGTAAGTC TGCCACAGTT AATCCAGAGT CAAAAACTGA AGCCAAAACC 
CTGGAGGACA TCTTTGAATC AAGCACTTTG TCTGATGGCC AAGCTATTGC AGACCAAAGT 
GAAGTCATAT CAACGTTGGG CTATTTGGAA AGGACACAGA ATGAGGATGA AGCAAAGAAA 
TATGTAAGTC CCTCTTTCCA ACCAGAGTTC TCTTCTGGGG CTGAGGAGGC ACTGACAGAT 
CCTACCCCCT ACGTAAGTAT TGGTACTACC TACCTTACGG CTCAGAGTTT AACAGAGGCA 
CCTGATGTGA TGGAAGGAGC CCGTCTGCCG GATTCCATCG ACACATCCAC AGTTTCAGCT 
TTTTCAGAGC TGCTCTCTCA GACACCATCA TTTCCTCCAC TCAGCATCCA CTTAGGCAGT 
GGAGACTCTG AGCACTCAGA GGACTTGCAG CCAAGTGCTC TGCCAAGTAC AGATGCCAGC 
ACACCCCCAG TATCCTCAGG AGAACTTGCA AACATTGAAG CGACTTTCAA ACCCTCAAGT 
GAAGAAGACT TTTACATAAC TGAGCCTCCA TCCTTACCTC CTGACACAGA GCCTTCAGAA 
GATGAAAGTA AGCCTAAACT ATTAGAACCA ACAGAGGCTT CTGCCACAGA ACTAATTGCT 
CAAGAAGAAA TTGAGATTTT CCAAAATTCT GACAACACAA CCAGTGTCCA GGTTTCTGGG 
GAAGCAGTCA AGGTGTTTCC CAGTATTGAA ACCCCTGAGG CTGAGGCTAT TGTCACAGCT 
GCAAGTGAAA CGAAGTTAGA AGGTGCTACA CTGCGGCCAC ATTCTACTTC TGCTTCTGTG 
ATTCACGGGG TCGAGGCAGG TGTGGTGCCT CAGCCCAGCC CACAGACTTC TGAGCGGCCC 
ACCATCCTTT CTCCTCTGGA AATAAGCCCT GAAACACAGG CAGCTTTGAT CAGAGGGGAG 
GATTCCACAG TAGCGGCCCC CAAACAGCAA GTACCGACGA GGATGCTTGA TTCTAATAAG 
CAGGCAACAC TAAGCACCAC AGAGTTAAAT ACTGAGCTTG CAACACCATC ATTTCCCCTT 
CTGGAAACTT CTAATGAAAC CAGTTTCCTG ATTGGCATTA ATGAAGAGTC AGTGGAAGGC 
ACAGCAGTCT ATTTACCAGG ACCTGATCGT TGCAAAATGA ACCCGTGCCT CAATGGGGGC 
ACCTGTTATC CTACTGAAAC TTCCTACGTG TGCACCTGCG TGCCAGGATA CAGTGGTGAT 
CGGTGTGAAC TTGATTTTGA TGAATGTCAT TCTAATCCTT GTCGCAATGG AGCCACATGT 
ATTGACGGTT TTAATACCTT CAGGTGTCTC TGCCTCCCGA GCTACGTTGG TGCGCTTTGT 
GAGCAAGACA CGGAGACATG TGACTATGGC TGGCACAAAT TTCAAGGGCA GTGCTACAAG 
TACTTTGCCC ATCGACGGAC GTGGGATGCA GCTGAACGGG AATGCCGTCT GCAGGGCGCC 
CATCTCACCA GTATCCTGTC TCACGAGGAA CAAATGTTTG TGAATCGTGT GGGCCATGAT 
TATCAGTGGA TAGGTCTCAA TGACAAGATG TTTGAGCATG ACTTCCGTTG GACTGATGGC 
AGCACACTGC AATATGAGAA CTGGAGGCCG AACCAACCAG ACAGCTTCTT TTCTACTGGA 
GAAGATTGTG TTGTGATTAT ATGGCATGAG AATGGCCAGT GGAATGACGT TCCCTGCAAT 
TACCATCTCA CCTACACCTG CAAGAAAGGA ACAGTTGCTT GCGGCCAGCC CCCTGTTGTA 
GAAAATGCCA AGACCTTTGG AAAGATGAAA CCTCGTTATG AAATCAACTC CTTGATTAGA 
TATCACTGCA AAGATGGTTT CATTCAACGC CACCTTCCAA CTATCCGTTG CCTAGGAAAT 
GGAAGATGGG CTATGCCTAA AATTACCTGC CTGAACCCAT CTGCATACCA AAGGACTTAT 
TCTAAGAAAT ATTTTAAAAA TTCCTCATCA GCAAAGGACA ATTCAATAAA TACATCAAAA 
CATGATCACC GTTGGAGCCG AAGGTGGCAA GAATCAAGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209776.2
CDS	296..7480
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPTPDSRFDAYCFKRRMSDFSVSGHPIDSESKEDEPCSEETDPEHDLIAEILPELLGMLHSEEDEEDEECANATDVTTTPSVQYINGKHVVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDSHQFIITHAGLPTAMQPNESKETTESLEITWRPEIYPETAEPFSSGEPDIFPTASIHEGEATEGPDSITEKNPELDHLVHEHAESVPLFPEESSGDAAIDQESQKIIFSGATEGTFGEEAEKSSTTHTPSMVASSVSAPVSEDASFILTGTPQSDEPLSTVESWVEITPRHTVEFSGSPSIPIPEGSGEAEEDKDKIFAMITDLSQRNTTDPRVTSDTSKIMITESLVDVPTTTVYSISEQVSAVVPTKFVRETDTYEWVFSPPLEETTRKEEEKGTTGTASTVEVHSPTQRLDQFVSPSELESSSETPPDDSAAATRKSFTSQMTPTQSERETTSSTVVFKETEVLDNLAAQTTDPSLSSQPGVLEVSPTVPGSPVSLFMEQGSGEAAVDPETTTVSSLSLNIEPEILAEEEAAGTWSPNVETVFPFEPTEQVLSTAVDREVAETISQTSKENLVSEISGEPTHRAEIKGFSTDFPLEEDFSGDFREYSTVSYPITKEEIVMMEGSGDAAFKDTQMLPSVTPTSDLSNHTADSEEPGSTLVSTSAFPWEEFTASAEGSGEPLLSVSSSVDQVFPSAAGKASGTDSPFIDQRLGEEGAINETDQRSTILPTAEAESTKASTEEGEVKENHTVSMDFPPTVEPDELWPRQEVNPVRQGNGSEIVSEEKTQEQESFEPLQSSVAPEQTTFDSQTFPEPGLQTTGYFTLTTKKTYSTDERMEEEVISLADVSTPTLDSKGLVLYTTLPEVTEKSHFFLATASVTESVPAESVIAGSTIKEEESIKPFPKVTSPIIKESDTDLIFSGLGSGEEVLPTLGSVNFTEIEQVLSTLYPLTSQVQSLEASILNDTSGDYEGMENVANEMRPLISKTDSIFEDGETASSTTLPEILSDARTEGPFTAPLTFSTGPGQPQNQTHRRAEEIQTSRPQPLTDQVSSENSVTAETKETATPATDFLARTYDLEMAKGFVTSTPKPSDLFYEHSGEGSGELDAVGAEVHASGMTQATRQGSTTFVSDRSLEKHPKVPSVEAVTVNGFPTVSMVLPLHPEQREGSPEATGTPASTASYEKATEGAADSFQDHFWGFKDSTLKPDKRKATESIIIDLDKEDKDLILTMTESTILEIIPELTSDKNTIIDIDHTKPIYEDILGMQTDLDPEVPSGPPDSSEESTQVQEKYEAAVNLSSTEENFEASGDILLANYTQATPESKAPEDRNPLDHTDFIFTTGIPILSSETELDVLLPTATSLPIPSKSATVNPESKTEAKTLEDIFESSTLSDGQAIADQSEVISTLGYLERTQNEDEAKKYVSPSFQPEFSSGAEEALTDPTPYVSIGTTYLTAQSLTEAPDVMEGARLPDSIDTSTVSAFSELLSQTPSFPPLSIHLGSGDSEHSEDLQPSALPSTDASTPPVSSGELANIEATFKPSSEEDFYITEPPSLPPDTEPSEDESKPKLLEPTEASATELIAQEEIEIFQNSDNTTSVQVSGEAVKVFPSIETPEAEAIVTAASETKLEGATLRPHSTSASVIHGVEAGVVPQPSPQTSERPTILSPLEISPETQAALIRGEDSTVAAPKQQVPTRMLDSNKQATLSTTELNTELATPSFPLLETSNETSFLIGINEESVEGTAVYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	XM_005209719.4
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209719.4

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGTCGA ATGAGTGATT TCAGTGTAAG TGGTCATCCA 
ATAGATTCAG AATCTAAAGA AGATGAGCCT TGTAGTGAAG AAACAGATCC AGAGCACGAT 
CTAATTGCTG AAATTTTACC TGAGTTACTT GGTATGTTGC ACAGTGAAGA GGATGAAGAA 
GATGAGGAGT GTGCCAATGC CACTGATGTC ACGACCACCC CATCCGTGCA GTACATAAAT 
GGGAAGCATG TAGTTACCAC TGTTCCCAAG GACCCAGAAG CAGCAGAAGC TAGGCGTGGC 
CAGTTCGAAA GTGTTGCACC TTCTCAAAAT TTTTCAGACA GCAGTGAAAG TGATAGCCAT 
CAGTTTATAA TCACCCATGC TGGACTGCCC ACTGCCATGC AACCTAATGA ATCTAAAGAA 
ACAACGGAGT CCCTTGAAAT TACGTGGAGA CCCGAGATTT ACCCTGAAAC AGCAGAGCCT 
TTCTCAAGTG GCGAGCCTGA TATTTTCCCC ACAGCCTCAA TCCATGAGGG AGAAGCCACT 
GAGGGGCCAG ACTCAATCAC AGAGAAAAAT CCTGAACTTG ATCATCTGGT GCATGAACAT 
GCTGAATCGG TACCTCTGTT TCCTGAAGAG TCTTCAGGAG ATGCTGCCAT TGACCAGGAA 
TCTCAAAAAA TCATCTTTTC AGGGGCTACC GAAGGAACGT TTGGTGAAGA GGCAGAAAAA 
AGTTCTACCA CACATACTCC AAGTATGGTT GCAAGTTCTG TATCAGCACC TGTCTCAGAG 
GATGCATCAT TTATCTTAAC AGGCACTCCA CAGTCTGATG AGCCGTTGTC CACAGTAGAA 
AGCTGGGTGG AAATAACCCC TAGACACACT GTCGAGTTCT CGGGAAGTCC TTCAATTCCA 
ATTCCGGAAG GCTCTGGGGA AGCAGAAGAA GACAAAGATA AAATATTTGC CATGATAACT 
GATTTATCAC AGAGAAATAC TACAGACCCC CGCGTTACTT CAGACACCAG CAAGATCATG 
ATCACGGAGA GCCTTGTGGA TGTTCCTACA ACCACTGTAT ATTCAATTTC TGAACAGGTT 
TCTGCAGTAG TGCCTACCAA ATTTGTAAGG GAGACAGACA CTTATGAGTG GGTTTTCAGT 
CCACCTCTGG AGGAAACAAC AAGGAAAGAG GAGGAGAAGG GAACTACAGG TACAGCTTCT 
ACAGTTGAGG TACATTCCCC TACACAGCGA TTAGATCAGT TTGTTTCACC CTCTGAGTTA 
GAAAGCTCAA GTGAAACTCC ACCTGATGAT TCAGCAGCTG CTACTAGGAA GAGTTTCACG 
TCCCAGATGA CACCCACACA GTCTGAAAGG GAAACGACAA GTTCTACTGT TGTCTTTAAA 
GAAACAGAAG TTTTAGACAA TCTGGCAGCA CAGACCACAG ATCCCAGCCT TAGCAGTCAA 
CCTGGGGTTC TGGAAGTGTC ACCTACTGTC CCAGGGAGCC CCGTCTCTCT CTTTATGGAG 
CAAGGCTCCG GAGAAGCTGC TGTTGACCCA GAAACCACCA CTGTTTCTTC ACTTTCATTA 
AATATAGAGC CTGAAATTCT AGCTGAAGAG GAGGCAGCTG GCACTTGGTC TCCAAATGTG 
GAAACTGTAT TCCCTTTTGA GCCAACCGAA CAAGTCTTAA GTACTGCAGT GGACAGGGAG 
GTTGCTGAAA CTATAAGCCA AACATCTAAG GAAAACTTGG TTTCAGAGAT CTCAGGAGAG 
CCAACTCACA GGGCAGAAAT AAAGGGCTTT TCTACAGATT TTCCTTTGGA GGAAGATTTC 
AGTGGTGACT TCAGAGAATA CTCGACAGTA TCTTATCCCA TAACAAAAGA AGAAATAGTG 
ATGATGGAAG GCTCAGGGGA TGCAGCATTT AAGGATACCC AGATGTTACC ATCTGTAACA 
CCTACCTCGG ACCTCAGTAA CCACACTGCT GACTCGGAAG AACCTGGCAG CACCTTGGTC 
AGCACCTCAG CCTTCCCTTG GGAAGAATTC ACAGCCTCTG CTGAGGGCTC AGGTGAGCCC 
TTGCTCTCAG TAAGCAGCTC TGTTGACCAA GTGTTTCCCA GTGCCGCGGG AAAAGCCTCT 
GGAACAGATT CCCCATTTAT TGACCAACGA TTGGGAGAAG AAGGTGCTAT CAATGAAACT 
GATCAAAGAT CCACCATTTT GCCAACAGCA GAAGCTGAGA GTACTAAAGC TTCAACCGAA 
GAGGGGGAGG TGAAGGAGAA TCACACAGTT TCAATGGACT TTCCCCCAAC TGTGGAGCCA 
GACGAATTAT GGCCTAGACA AGAAGTCAAC CCTGTAAGGC AAGGAAATGG AAGTGAAATA 
GTATCAGAGG AAAAGACTCA AGAGCAAGAA TCTTTTGAAC CCCTTCAAAG CTCTGTTGCA 
CCAGAACAAA CAACTTTTGA TTCACAGACA TTTCCTGAAC CTGGACTCCA AACCACAGGT 
TATTTCACAC TAACGACAAA GAAAACTTAC AGTACTGATG AGAGAATGGA GGAGGAAGTC 
ATTTCCTTAG CTGACGTGTC CACTCCAACT CTAGATTCAA AGGGCTTGGT ATTGTACACT 
ACTCTCCCTG AAGTTACTGA AAAATCCCAT TTTTTCTTAG CTACTGCCTC AGTGACTGAA 
TCAGTACCAG CTGAAAGTGT AATTGCAGGT TCAACCATCA AAGAGGAAGA GAGTATAAAA 
CCCTTTCCCA AAGTTACGAG CCCGATAATT AAAGAGTCAG ATACAGATCT TATATTCTCT 
GGACTGGGAT CTGGGGAAGA AGTTTTGCCT ACTCTAGGAT CAGTGAATTT TACTGAAATA 
GAACAAGTCC TTAGCACATT ATATCCCCTG ACTTCTCAAG TACAAAGTTT AGAAGCCAGC 
ATCTTAAATG ATACAAGTGG AGACTATGAG GGAATGGAAA ATGTAGCAAA TGAAATGAGA 
CCACTCATTT CCAAAACAGA CAGCATTTTT GAAGATGGTG AGACAGCATC CAGCACAACA 
CTACCAGAAA TTTTAAGTGA CGCCAGAACA GAAGGGCCCT TCACGGCACC TCTCACTTTT 
TCCACAGGCC CCGGACAGCC TCAAAATCAG ACGCACCGTA GGGCAGAAGA AATCCAGACC 
AGTAGACCAC AGCCTCTGAC TGATCAAGTC TCTAGCGAGA ATTCTGTGAC AGCAGAAACT 
AAAGAAACAG CAACCCCTGC CACTGATTTT CTGGCTAGAA CATATGATCT TGAAATGGCC 
AAAGGATTTG TTACATCAAC ACCGAAACCA TCTGACCTGT TTTATGAACA TTCTGGAGAA 
GGTTCTGGGG AATTGGATGC CGTTGGTGCA GAAGTCCACG CTTCTGGAAT GACTCAGGCA 
ACTAGGCAAG GAAGCACCAC ATTTGTTTCT GATAGATCCC TGGAAAAACA TCCCAAGGTT 
CCAAGTGTCG AAGCTGTTAC TGTTAATGGA TTCCCGACAG TTTCAATGGT GCTGCCGCTT 
CATCCTGAGC AGAGAGAAGG CTCTCCTGAA GCAACCGGCA CACCGGCAAG CACAGCATCA 
TATGAGAAGG CCACAGAAGG TGCGGCAGAT AGTTTCCAAG ACCATTTCTG GGGATTCAAG 
GATTCCACGC TGAAACCCGA CAAAAGAAAA GCCACTGAAA GTATTATTAT AGATCTGGAC 
AAAGAGGACA AGGATTTAAT ATTGACAATG ACGGAGAGTA CCATCCTTGA AATTATACCT 
GAGCTCACAT CAGATAAAAA TACTATCATA GATATTGATC ACACTAAACC AATATATGAA 
GACATCCTTG GAATGCAGAC GGACTTGGAT CCAGAAGTAC CCTCAGGACC GCCTGACAGT 
AGTGAAGAGA GCACTCAAGT TCAAGAGAAG TATGAGGCAG CTGTTAACCT GTCTTCAACT 
GAGGAAAACT TTGAGGCTTC TGGTGATATT CTGCTGGCTA ATTACACTCA GGCAACACCT 
GAATCGAAGG CTCCTGAAGA CAGAAATCCA TTAGATCACA CAGACTTTAT CTTCACGACT 
GGGATCCCCA TTCTTAGCTC AGAGACAGAA TTAGACGTTT TGCTTCCCAC AGCCACATCT 
CTGCCCATTC CTAGTAAGTC TGCCACAGTT AATCCAGAGT CAAAAACTGA AGCCAAAACC 
CTGGAGGACA TCTTTGAATC AAGCACTTTG TCTGATGGCC AAGCTATTGC AGACCAAAGT 
GAAGTCATAT CAACGTTGGG CTATTTGGAA AGGACACAGA ATGAGGATGA AGCAAAGAAA 
TATGTAAGTC CCTCTTTCCA ACCAGAGTTC TCTTCTGGGG CTGAGGAGGC ACTGACAGAT 
CCTACCCCCT ACGTAAGTAT TGGTACTACC TACCTTACGG CTCAGAGTTT AACAGAGGCA 
CCTGATGTGA TGGAAGGAGC CCGTCTGCCG GATTCCATCG ACACATCCAC AGTTTCAGCT 
TTTTCAGAGC TGCTCTCTCA GACACCATCA TTTCCTCCAC TCAGCATCCA CTTAGGCAGT 
GGAGACTCTG AGCACTCAGA GGACTTGCAG CCAAGTGCTC TGCCAAGTAC AGATGCCAGC 
ACACCCCCAG TATCCTCAGG AGAACTTGCA AACATTGAAG CGACTTTCAA ACCCTCAAGT 
GAAGAAGACT TTTACATAAC TGAGCCTCCA TCCTTACCTC CTGACACAGA GCCTTCAGAA 
GATGAAAGTA AGCCTAAACT ATTAGAACCA ACAGAGGCTT CTGCCACAGA ACTAATTGCT 
CAAGAAGAAA TTGAGATTTT CCAAAATTCT GACAACACAA CCAGTGTCCA GGTTTCTGGG 
GAAGCAGTCA AGGTGTTTCC CAGTATTGAA ACCCCTGAGG CTGAGGCTAT TGTCACAGCT 
GCAAGTGAAA CGAAGTTAGA AGGTGCTACA CTGCGGCCAC ATTCTACTTC TGCTTCTGTG 
ATTCACGGGG TCGAGGCAGG TGTGGTGCCT CAGCCCAGCC CACAGACTTC TGAGCGGCCC 
ACCATCCTTT CTCCTCTGGA AATAAGCCCT GAAACACAGG CAGCTTTGAT CAGAGGGGAG 
GATTCCACAG TAGCGGCCCC CAAACAGCAA GTACCGACGA GGATGCTTGA TTCTAATAAG 
CAGGCAACAC TAAGCACCAC AGAGTTAAAT ACTGAGCTTG CAACACCATC ATTTCCCCTT 
CTGGAAACTT CTAATGAAAC CAGTTTCCTG ATTGGCATTA ATGAAGAGTC AGTGGAAGGC 
ACAGCAGTCT ATTTACCAGG ACCTGATCGT TGCAAAATGA ACCCGTGCCT CAATGGGGGC 
ACCTGTTATC CTACTGAAAC TTCCTACGTG TGCACCTGCG TGCCAGGATA CAGTGGTGAT 
CGGTGTGAAC TTGATTTTGA TGAATGTCAT TCTAATCCTT GTCGCAATGG AGCCACATGT 
ATTGACGGTT TTAATACCTT CAGGTGTCTC TGCCTCCCGA GCTACGTTGG TGCGCTTTGT 
GAGCAAGACA CGGAGACATG TGACTATGGC TGGCACAAAT TTCAAGGGCA GTGCTACAAG 
TACTTTGCCC ATCGACGGAC GTGGGATGCA GCTGAACGGG AATGCCGTCT GCAGGGCGCC 
CATCTCACCA GTATCCTGTC TCACGAGGAA CAAATGTTTG TGAATCGTGT GGGCCATGAT 
TATCAGTGGA TAGGTCTCAA TGACAAGATG TTTGAGCATG ACTTCCGTTG GACTGATGGC 
AGCACACTGC AATATGAGAA CTGGAGGCCG AACCAACCAG ACAGCTTCTT TTCTACTGGA 
GAAGATTGTG TTGTGATTAT ATGGCATGAG AATGGCCAGT GGAATGACGT TCCCTGCAAT 
TACCATCTCA CCTACACCTG CAAGAAAGGA ACAGTTGCTT GCGGCCAGCC CCCTGTTGTA 
GAAAATGCCA AGACCTTTGG AAAGATGAAA CCTCGTTATG AAATCAACTC CTTGATTAGA 
TATCACTGCA AAGATGGTTT CATTCAACGC CACCTTCCAA CTATCCGTTG CCTAGGAAAT 
GGAAGATGGG CTATGCCTAA AATTACCTGC CTGAACCCAT CTGCATACCA AAGGACTTAT 
TCTAAGAAAT ATTTTAAAAA TTCCTCATCA GCAAAGGACA ATTCAATAAA TACATCAAAA 
CATGATCACC GTTGGAGCCG AAGGTGGCAA GAATCAAGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( XM_005209719.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209776.1 Bos taurus versican (VCAN), transcript variant X1, mRNA.

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CloneID	OBa05605
Clone ID Related Accession (Same CDS sequence)	XM_005209719.3
Accession Version	XM_005209719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	versican core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104055.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209719.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTCC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGTCGA ATGAGTGATT TCAGTGTAAG TGGTCATCCA 
ATAGATTCAG AATCTAAAGA AGATGAGCCT TGTAGTGAAG AAACAGATCC AGAGCACGAT 
CTAATTGCTG AAATTTTACC TGAGTTACTT GGTATGTTGC ACAGTGAAGA GGATGAAGAA 
GATGAGGAGT GTGCCAATGC CACTGATGTC ACGACCACCC CATCCGTGCA GTACATAAAT 
GGGAAGCATG TAGTTACCAC TGTTCCCAAG GACCCAGAAG CAGCAGAAGC TAGGCGTGGC 
CAGTTCGAAA GTGTTGCACC TTCTCAAAAT TTTTCAGACA GCAGTGAAAG TGATAGCCAT 
CAGTTTATAA TCACCCATGC TGGACTGCCC ACTGCCATGC AACCTAATGA ATCTAAAGAA 
ACAACGGAGT CCCTTGAAAT TACGTGGAGA CCCGAGATTT ACCCTGAAAC AGCAGAGCCT 
TTCTCAAGTG GCGAGCCTGA TATTTTCCCC ACAGCCTCAA TCCATGAGGG AGAAGCCACT 
GAGGGGCCAG ACTCAATCAC AGAGAAAAAT CCTGAACTTG ATCATCTGGT GCATGAACAT 
GCTGAATCGG TACCTCTGTT TCCTGAAGAG TCTTCAGGAG ATGCTGCCAT TGACCAGGAA 
TCTCAAAAAA TCATCTTTTC AGGGGCTACC GAAGGAACGT TTGGTGAAGA GGCAGAAAAA 
AGTTCTACCA CACATACTCC AAGTATGGTT GCAAGTTCTG TATCAGCACC TGTCTCAGAG 
GATGCATCAT TTATCTTAAC AGGCACTCCA CAGTCTGATG AGCCGTTGTC CACAGTAGAA 
AGCTGGGTGG AAATAACCCC TAGACACACT GTCGAGTTCT CGGGAAGTCC TTCAATTCCA 
ATTCCGGAAG GCTCTGGGGA AGCAGAAGAA GACAAAGATA AAATATTTGC CATGATAACT 
GATTTATCAC AGAGAAATAC TACAGACCCC CGCGTTACTT CAGACACCAG CAAGATCATG 
ATCACGGAGA GCCTTGTGGA TGTTCCTACA ACCACTGTAT ATTCAATTTC TGAACAGGTT 
TCTGCAGTAG TGCCTACCAA ATTTGTAAGG GAGACAGACA CTTATGAGTG GGTTTTCAGT 
CCACCTCTGG AGGAAACAAC AAGGAAAGAG GAGGAGAAGG GAACTACAGG TACAGCTTCT 
ACAGTTGAGG TACATTCCCC TACACAGCGA TTAGATCAGT TTGTTTCACC CTCTGAGTTA 
GAAAGCTCAA GTGAAACTCC ACCTGATGAT TCAGCAGCTG CTACTAGGAA GAGTTTCACG 
TCCCAGATGA CACCCACACA GTCTGAAAGG GAAACGACAA GTTCTACTGT TGTCTTTAAA 
GAAACAGAAG TTTTAGACAA TCTGGCAGCA CAGACCACAG ATCCCAGCCT TAGCAGTCAA 
CCTGGGGTTC TGGAAGTGTC ACCTACTGTC CCAGGGAGCC CCGTCTCTCT CTTTATGGAG 
CAAGGCTCCG GAGAAGCTGC TGTTGACCCA GAAACCACCA CTGTTTCTTC ACTTTCATTA 
AATATAGAGC CTGAAATTCT AGCTGAAGAG GAGGCAGCTG GCACTTGGTC TCCAAATGTG 
GAAACTGTAT TCCCTTTTGA GCCAACCGAA CAAGTCTTAA GTACTGCAGT GGACAGGGAG 
GTTGCTGAAA CTATAAGCCA AACATCTAAG GAAAACTTGG TTTCAGAGAT CTCAGGAGAG 
CCAACTCACA GGGCAGAAAT AAAGGGCTTT TCTACAGATT TTCCTTTGGA GGAAGATTTC 
AGTGGTGACT TCAGAGAATA CTCGACAGTA TCTTATCCCA TAACAAAAGA AGAAATAGTG 
ATGATGGAAG GCTCAGGGGA TGCAGCATTT AAGGATACCC AGATGTTACC ATCTGTAACA 
CCTACCTCGG ACCTCAGTAA CCACACTGCT GACTCGGAAG AACCTGGCAG CACCTTGGTC 
AGCACCTCAG CCTTCCCTTG GGAAGAATTC ACAGCCTCTG CTGAGGGCTC AGGTGAGCCC 
TTGCTCTCAG TAAGCAGCTC TGTTGACCAA GTGTTTCCCA GTGCCGCGGG AAAAGCCTCT 
GGAACAGATT CCCCATTTAT TGACCAACGA TTGGGAGAAG AAGGTGCTAT CAATGAAACT 
GATCAAAGAT CCACCATTTT GCCAACAGCA GAAGCTGAGA GTACTAAAGC TTCAACCGAA 
GAGGGGGAGG TGAAGGAGAA TCACACAGTT TCAATGGACT TTCCCCCAAC TGTGGAGCCA 
GACGAATTAT GGCCTAGACA AGAAGTCAAC CCTGTAAGGC AAGGAAATGG AAGTGAAATA 
GTATCAGAGG AAAAGACTCA AGAGCAAGAA TCTTTTGAAC CCCTTCAAAG CTCTGTTGCA 
CCAGAACAAA CAACTTTTGA TTCACAGACA TTTCCTGAAC CTGGACTCCA AACCACAGGT 
TATTTCACAC TAACGACAAA GAAAACTTAC AGTACTGATG AGAGAATGGA GGAGGAAGTC 
ATTTCCTTAG CTGACGTGTC CACTCCAACT CTAGATTCAA AGGGCTTGGT ATTGTACACT 
ACTCTCCCTG AAGTTACTGA AAAATCCCAT TTTTTCTTAG CTACTGCCTC AGTGACTGAA 
TCAGTACCAG CTGAAAGTGT AATTGCAGGT TCAACCATCA AAGAGGAAGA GAGTATAAAA 
CCCTTTCCCA AAGTTACGAG CCCGATAATT AAAGAGTCAG ATACAGATCT TATATTCTCT 
GGACTGGGAT CTGGGGAAGA AGTTTTGCCT ACTCTAGGAT CAGTGAATTT TACTGAAATA 
GAACAAGTCC TTAGCACATT ATATCCCCTG ACTTCTCAAG TACAAAGTTT AGAAGCCAGC 
ATCTTAAATG ATACAAGTGG AGACTATGAG GGAATGGAAA ATGTAGCAAA TGAAATGAGA 
CCACTCATTT CCAAAACAGA CAGCATTTTT GAAGATGGTG AGACAGCATC CAGCACAACA 
CTACCAGAAA TTTTAAGTGA CGCCAGAACA GAAGGGCCCT TCACGGCACC TCTCACTTTT 
TCCACAGGCC CCGGACAGCC TCAAAATCAG ACGCACCGTA GGGCAGAAGA AATCCAGACC 
AGTAGACCAC AGCCTCTGAC TGATCAAGTC TCTAGCGAGA ATTCTGTGAC AGCAGAAACT 
AAAGAAACAG CAACCCCTGC CACTGATTTT CTGGCTAGAA CATATGATCT TGAAATGGCC 
AAAGGATTTG TTACATCAAC ACCGAAACCA TCTGACCTGT TTTATGAACA TTCTGGAGAA 
GGTTCTGGGG AATTGGATGC CGTTGGTGCA GAAGTCCACG CTTCTGGAAT GACTCAGGCA 
ACTAGGCAAG GAAGCACCAC ATTTGTTTCT GATAGATCCC TGGAAAAACA TCCCAAGGTT 
CCAAGTGTCG AAGCTGTTAC TGTTAATGGA TTCCCGACAG TTTCAATGGT GCTGCCGCTT 
CATCCTGAGC AGAGAGAAGG CTCTCCTGAA GCAACCGGCA CACCGGCAAG CACAGCATCA 
TATGAGAAGG CCACAGAAGG TGCGGCAGAT AGTTTCCAAG ACCATTTCTG GGGATTCAAG 
GATTCCACGC TGAAACCCGA CAAAAGAAAA GCCACTGAAA GTATTATTAT AGATCTGGAC 
AAAGAGGACA AGGATTTAAT ATTGACAATG ACGGAGAGTA CCATCCTTGA AATTATACCT 
GAGCTCACAT CAGATAAAAA TACTATCATA GATATTGATC ACACTAAACC AATATATGAA 
GACATCCTTG GAATGCAGAC GGACTTGGAT CCAGAAGTAC CCTCAGGACC GCCTGACAGT 
AGTGAAGAGA GCACTCAAGT TCAAGAGAAG TATGAGGCAG CTGTTAACCT GTCTTCAACT 
GAGGAAAACT TTGAGGCTTC TGGTGATATT CTGCTGGCTA ATTACACTCA GGCAACACCT 
GAATCGAAGG CTCCTGAAGA CAGAAATCCA TTAGATCACA CAGACTTTAT CTTCACGACT 
GGGATCCCCA TTCTTAGCTC AGAGACAGAA TTAGACGTTT TGCTTCCCAC AGCCACATCT 
CTGCCCATTC CTAGTAAGTC TGCCACAGTT AATCCAGAGT CAAAAACTGA AGCCAAAACC 
CTGGAGGACA TCTTTGAATC AAGCACTTTG TCTGATGGCC AAGCTATTGC AGACCAAAGT 
GAAGTCATAT CAACGTTGGG CTATTTGGAA AGGACACAGA ATGAGGATGA AGCAAAGAAA 
TATGTAAGTC CCTCTTTCCA ACCAGAGTTC TCTTCTGGGG CTGAGGAGGC ACTGACAGAT 
CCTACCCCCT ACGTAAGTAT TGGTACTACC TACCTTACGG CTCAGAGTTT AACAGAGGCA 
CCTGATGTGA TGGAAGGAGC CCGTCTGCCG GATTCCATCG ACACATCCAC AGTTTCAGCT 
TTTTCAGAGC TGCTCTCTCA GACACCATCA TTTCCTCCAC TCAGCATCCA CTTAGGCAGT 
GGAGACTCTG AGCACTCAGA GGACTTGCAG CCAAGTGCTC TGCCAAGTAC AGATGCCAGC 
ACACCCCCAG TATCCTCAGG AGAACTTGCA AACATTGAAG CGACTTTCAA ACCCTCAAGT 
GAAGAAGACT TTTACATAAC TGAGCCTCCA TCCTTACCTC CTGACACAGA GCCTTCAGAA 
GATGAAAGTA AGCCTAAACT ATTAGAACCA ACAGAGGCTT CTGCCACAGA ACTAATTGCT 
CAAGAAGAAA TTGAGATTTT CCAAAATTCT GACAACACAA CCAGTGTCCA GGTTTCTGGG 
GAAGCAGTCA AGGTGTTTCC CAGTATTGAA ACCCCTGAGG CTGAGGCTAT TGTCACAGCT 
GCAAGTGAAA CGAAGTTAGA AGGTGCTACA CTGCGGCCAC ATTCTACTTC TGCTTCTGTG 
ATTCACGGGG TCGAGGCAGG TGTGGTGCCT CAGCCCAGCC CACAGACTTC TGAGCGGCCC 
ACCATCCTTT CTCCTCTGGA AATAAGCCCT GAAACACAGG CAGCTTTGAT CAGAGGGGAG 
GATTCCACAG TAGCGGCCCC CAAACAGCAA GTACCGACGA GGATGCTTGA TTCTAATAAG 
CAGGCAACAC TAAGCACCAC AGAGTTAAAT ACTGAGCTTG CAACACCATC ATTTCCCCTT 
CTGGAAACTT CTAATGAAAC CAGTTTCCTG ATTGGCATTA ATGAAGAGTC AGTGGAAGGC 
ACAGCAGTCT ATTTACCAGG ACCTGATCGT TGCAAAATGA ACCCGTGCCT CAATGGGGGC 
ACCTGTTATC CTACTGAAAC TTCCTACGTG TGCACCTGCG TGCCAGGATA CAGTGGTGAT 
CGGTGTGAAC TTGATTTTGA TGAATGTCAT TCTAATCCTT GTCGCAATGG AGCCACATGT 
ATTGACGGTT TTAATACCTT CAGGTGTCTC TGCCTCCCGA GCTACGTTGG TGCGCTTTGT 
GAGCAAGACA CGGAGACATG TGACTATGGC TGGCACAAAT TTCAAGGGCA GTGCTACAAG 
TACTTTGCCC ATCGACGGAC GTGGGATGCA GCTGAACGGG AATGCCGTCT GCAGGGCGCC 
CATCTCACCA GTATCCTGTC TCACGAGGAA CAAATGTTTG TGAATCGTGT GGGCCATGAT 
TATCAGTGGA TAGGTCTCAA TGACAAGATG TTTGAGCATG ACTTCCGTTG GACTGATGGC 
AGCACACTGC AATATGAGAA CTGGAGGCCG AACCAACCAG ACAGCTTCTT TTCTACTGGA 
GAAGATTGTG TTGTGATTAT ATGGCATGAG AATGGCCAGT GGAATGACGT TCCCTGCAAT 
TACCATCTCA CCTACACCTG CAAGAAAGGA ACAGTTGCTT GCGGCCAGCC CCCTGTTGTA 
GAAAATGCCA AGACCTTTGG AAAGATGAAA CCTCGTTATG AAATCAACTC CTTGATTAGA 
TATCACTGCA AAGATGGTTT CATTCAACGC CACCTTCCAA CTATCCGTTG CCTAGGAAAT 
GGAAGATGGG CTATGCCTAA AATTACCTGC CTGAACCCAT CTGCATACCA AAGGACTTAT 
TCTAAGAAAT ATTTTAAAAA TTCCTCATCA GCAAAGGACA ATTCAATAAA TACATCAAAA 
CATGATCACC GTTGGAGCCG AAGGTGGCAA GAATCAAGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209776.1
CDS	273..7457
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKRRMSDFSVSGHPIDSESKEDEPCSEETDPEHDLIAEILPELLGMLHSEEDEEDEECANATDVTTTPSVQYINGKHVVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDSHQFIITHAGLPTAMQPNESKETTESLEITWRPEIYPETAEPFSSGEPDIFPTASIHEGEATEGPDSITEKNPELDHLVHEHAESVPLFPEESSGDAAIDQESQKIIFSGATEGTFGEEAEKSSTTHTPSMVASSVSAPVSEDASFILTGTPQSDEPLSTVESWVEITPRHTVEFSGSPSIPIPEGSGEAEEDKDKIFAMITDLSQRNTTDPRVTSDTSKIMITESLVDVPTTTVYSISEQVSAVVPTKFVRETDTYEWVFSPPLEETTRKEEEKGTTGTASTVEVHSPTQRLDQFVSPSELESSSETPPDDSAAATRKSFTSQMTPTQSERETTSSTVVFKETEVLDNLAAQTTDPSLSSQPGVLEVSPTVPGSPVSLFMEQGSGEAAVDPETTTVSSLSLNIEPEILAEEEAAGTWSPNVETVFPFEPTEQVLSTAVDREVAETISQTSKENLVSEISGEPTHRAEIKGFSTDFPLEEDFSGDFREYSTVSYPITKEEIVMMEGSGDAAFKDTQMLPSVTPTSDLSNHTADSEEPGSTLVSTSAFPWEEFTASAEGSGEPLLSVSSSVDQVFPSAAGKASGTDSPFIDQRLGEEGAINETDQRSTILPTAEAESTKASTEEGEVKENHTVSMDFPPTVEPDELWPRQEVNPVRQGNGSEIVSEEKTQEQESFEPLQSSVAPEQTTFDSQTFPEPGLQTTGYFTLTTKKTYSTDERMEEEVISLADVSTPTLDSKGLVLYTTLPEVTEKSHFFLATASVTESVPAESVIAGSTIKEEESIKPFPKVTSPIIKESDTDLIFSGLGSGEEVLPTLGSVNFTEIEQVLSTLYPLTSQVQSLEASILNDTSGDYEGMENVANEMRPLISKTDSIFEDGETASSTTLPEILSDARTEGPFTAPLTFSTGPGQPQNQTHRRAEEIQTSRPQPLTDQVSSENSVTAETKETATPATDFLARTYDLEMAKGFVTSTPKPSDLFYEHSGEGSGELDAVGAEVHASGMTQATRQGSTTFVSDRSLEKHPKVPSVEAVTVNGFPTVSMVLPLHPEQREGSPEATGTPASTASYEKATEGAADSFQDHFWGFKDSTLKPDKRKATESIIIDLDKEDKDLILTMTESTILEIIPELTSDKNTIIDIDHTKPIYEDILGMQTDLDPEVPSGPPDSSEESTQVQEKYEAAVNLSSTEENFEASGDILLANYTQATPESKAPEDRNPLDHTDFIFTTGIPILSSETELDVLLPTATSLPIPSKSATVNPESKTEAKTLEDIFESSTLSDGQAIADQSEVISTLGYLERTQNEDEAKKYVSPSFQPEFSSGAEEALTDPTPYVSIGTTYLTAQSLTEAPDVMEGARLPDSIDTSTVSAFSELLSQTPSFPPLSIHLGSGDSEHSEDLQPSALPSTDASTPPVSSGELANIEATFKPSSEEDFYITEPPSLPPDTEPSEDESKPKLLEPTEASATELIAQEEIEIFQNSDNTTSVQVSGEAVKVFPSIETPEAEAIVTAASETKLEGATLRPHSTSASVIHGVEAGVVPQPSPQTSERPTILSPLEISPETQAALIRGEDSTVAAPKQQVPTRMLDSNKQATLSTTELNTELATPSFPLLETSNETSFLIGINEESVEGTAVYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	XM_005209719.3
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209719.3

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGC 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTCC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACGTCGA ATGAGTGATT TCAGTGTAAG TGGTCATCCA 
ATAGATTCAG AATCTAAAGA AGATGAGCCT TGTAGTGAAG AAACAGATCC AGAGCACGAT 
CTAATTGCTG AAATTTTACC TGAGTTACTT GGTATGTTGC ACAGTGAAGA GGATGAAGAA 
GATGAGGAGT GTGCCAATGC CACTGATGTC ACGACCACCC CATCCGTGCA GTACATAAAT 
GGGAAGCATG TAGTTACCAC TGTTCCCAAG GACCCAGAAG CAGCAGAAGC TAGGCGTGGC 
CAGTTCGAAA GTGTTGCACC TTCTCAAAAT TTTTCAGACA GCAGTGAAAG TGATAGCCAT 
CAGTTTATAA TCACCCATGC TGGACTGCCC ACTGCCATGC AACCTAATGA ATCTAAAGAA 
ACAACGGAGT CCCTTGAAAT TACGTGGAGA CCCGAGATTT ACCCTGAAAC AGCAGAGCCT 
TTCTCAAGTG GCGAGCCTGA TATTTTCCCC ACAGCCTCAA TCCATGAGGG AGAAGCCACT 
GAGGGGCCAG ACTCAATCAC AGAGAAAAAT CCTGAACTTG ATCATCTGGT GCATGAACAT 
GCTGAATCGG TACCTCTGTT TCCTGAAGAG TCTTCAGGAG ATGCTGCCAT TGACCAGGAA 
TCTCAAAAAA TCATCTTTTC AGGGGCTACC GAAGGAACGT TTGGTGAAGA GGCAGAAAAA 
AGTTCTACCA CACATACTCC AAGTATGGTT GCAAGTTCTG TATCAGCACC TGTCTCAGAG 
GATGCATCAT TTATCTTAAC AGGCACTCCA CAGTCTGATG AGCCGTTGTC CACAGTAGAA 
AGCTGGGTGG AAATAACCCC TAGACACACT GTCGAGTTCT CGGGAAGTCC TTCAATTCCA 
ATTCCGGAAG GCTCTGGGGA AGCAGAAGAA GACAAAGATA AAATATTTGC CATGATAACT 
GATTTATCAC AGAGAAATAC TACAGACCCC CGCGTTACTT CAGACACCAG CAAGATCATG 
ATCACGGAGA GCCTTGTGGA TGTTCCTACA ACCACTGTAT ATTCAATTTC TGAACAGGTT 
TCTGCAGTAG TGCCTACCAA ATTTGTAAGG GAGACAGACA CTTATGAGTG GGTTTTCAGT 
CCACCTCTGG AGGAAACAAC AAGGAAAGAG GAGGAGAAGG GAACTACAGG TACAGCTTCT 
ACAGTTGAGG TACATTCCCC TACACAGCGA TTAGATCAGT TTGTTTCACC CTCTGAGTTA 
GAAAGCTCAA GTGAAACTCC ACCTGATGAT TCAGCAGCTG CTACTAGGAA GAGTTTCACG 
TCCCAGATGA CACCCACACA GTCTGAAAGG GAAACGACAA GTTCTACTGT TGTCTTTAAA 
GAAACAGAAG TTTTAGACAA TCTGGCAGCA CAGACCACAG ATCCCAGCCT TAGCAGTCAA 
CCTGGGGTTC TGGAAGTGTC ACCTACTGTC CCAGGGAGCC CCGTCTCTCT CTTTATGGAG 
CAAGGCTCCG GAGAAGCTGC TGTTGACCCA GAAACCACCA CTGTTTCTTC ACTTTCATTA 
AATATAGAGC CTGAAATTCT AGCTGAAGAG GAGGCAGCTG GCACTTGGTC TCCAAATGTG 
GAAACTGTAT TCCCTTTTGA GCCAACCGAA CAAGTCTTAA GTACTGCAGT GGACAGGGAG 
GTTGCTGAAA CTATAAGCCA AACATCTAAG GAAAACTTGG TTTCAGAGAT CTCAGGAGAG 
CCAACTCACA GGGCAGAAAT AAAGGGCTTT TCTACAGATT TTCCTTTGGA GGAAGATTTC 
AGTGGTGACT TCAGAGAATA CTCGACAGTA TCTTATCCCA TAACAAAAGA AGAAATAGTG 
ATGATGGAAG GCTCAGGGGA TGCAGCATTT AAGGATACCC AGATGTTACC ATCTGTAACA 
CCTACCTCGG ACCTCAGTAA CCACACTGCT GACTCGGAAG AACCTGGCAG CACCTTGGTC 
AGCACCTCAG CCTTCCCTTG GGAAGAATTC ACAGCCTCTG CTGAGGGCTC AGGTGAGCCC 
TTGCTCTCAG TAAGCAGCTC TGTTGACCAA GTGTTTCCCA GTGCCGCGGG AAAAGCCTCT 
GGAACAGATT CCCCATTTAT TGACCAACGA TTGGGAGAAG AAGGTGCTAT CAATGAAACT 
GATCAAAGAT CCACCATTTT GCCAACAGCA GAAGCTGAGA GTACTAAAGC TTCAACCGAA 
GAGGGGGAGG TGAAGGAGAA TCACACAGTT TCAATGGACT TTCCCCCAAC TGTGGAGCCA 
GACGAATTAT GGCCTAGACA AGAAGTCAAC CCTGTAAGGC AAGGAAATGG AAGTGAAATA 
GTATCAGAGG AAAAGACTCA AGAGCAAGAA TCTTTTGAAC CCCTTCAAAG CTCTGTTGCA 
CCAGAACAAA CAACTTTTGA TTCACAGACA TTTCCTGAAC CTGGACTCCA AACCACAGGT 
TATTTCACAC TAACGACAAA GAAAACTTAC AGTACTGATG AGAGAATGGA GGAGGAAGTC 
ATTTCCTTAG CTGACGTGTC CACTCCAACT CTAGATTCAA AGGGCTTGGT ATTGTACACT 
ACTCTCCCTG AAGTTACTGA AAAATCCCAT TTTTTCTTAG CTACTGCCTC AGTGACTGAA 
TCAGTACCAG CTGAAAGTGT AATTGCAGGT TCAACCATCA AAGAGGAAGA GAGTATAAAA 
CCCTTTCCCA AAGTTACGAG CCCGATAATT AAAGAGTCAG ATACAGATCT TATATTCTCT 
GGACTGGGAT CTGGGGAAGA AGTTTTGCCT ACTCTAGGAT CAGTGAATTT TACTGAAATA 
GAACAAGTCC TTAGCACATT ATATCCCCTG ACTTCTCAAG TACAAAGTTT AGAAGCCAGC 
ATCTTAAATG ATACAAGTGG AGACTATGAG GGAATGGAAA ATGTAGCAAA TGAAATGAGA 
CCACTCATTT CCAAAACAGA CAGCATTTTT GAAGATGGTG AGACAGCATC CAGCACAACA 
CTACCAGAAA TTTTAAGTGA CGCCAGAACA GAAGGGCCCT TCACGGCACC TCTCACTTTT 
TCCACAGGCC CCGGACAGCC TCAAAATCAG ACGCACCGTA GGGCAGAAGA AATCCAGACC 
AGTAGACCAC AGCCTCTGAC TGATCAAGTC TCTAGCGAGA ATTCTGTGAC AGCAGAAACT 
AAAGAAACAG CAACCCCTGC CACTGATTTT CTGGCTAGAA CATATGATCT TGAAATGGCC 
AAAGGATTTG TTACATCAAC ACCGAAACCA TCTGACCTGT TTTATGAACA TTCTGGAGAA 
GGTTCTGGGG AATTGGATGC CGTTGGTGCA GAAGTCCACG CTTCTGGAAT GACTCAGGCA 
ACTAGGCAAG GAAGCACCAC ATTTGTTTCT GATAGATCCC TGGAAAAACA TCCCAAGGTT 
CCAAGTGTCG AAGCTGTTAC TGTTAATGGA TTCCCGACAG TTTCAATGGT GCTGCCGCTT 
CATCCTGAGC AGAGAGAAGG CTCTCCTGAA GCAACCGGCA CACCGGCAAG CACAGCATCA 
TATGAGAAGG CCACAGAAGG TGCGGCAGAT AGTTTCCAAG ACCATTTCTG GGGATTCAAG 
GATTCCACGC TGAAACCCGA CAAAAGAAAA GCCACTGAAA GTATTATTAT AGATCTGGAC 
AAAGAGGACA AGGATTTAAT ATTGACAATG ACGGAGAGTA CCATCCTTGA AATTATACCT 
GAGCTCACAT CAGATAAAAA TACTATCATA GATATTGATC ACACTAAACC AATATATGAA 
GACATCCTTG GAATGCAGAC GGACTTGGAT CCAGAAGTAC CCTCAGGACC GCCTGACAGT 
AGTGAAGAGA GCACTCAAGT TCAAGAGAAG TATGAGGCAG CTGTTAACCT GTCTTCAACT 
GAGGAAAACT TTGAGGCTTC TGGTGATATT CTGCTGGCTA ATTACACTCA GGCAACACCT 
GAATCGAAGG CTCCTGAAGA CAGAAATCCA TTAGATCACA CAGACTTTAT CTTCACGACT 
GGGATCCCCA TTCTTAGCTC AGAGACAGAA TTAGACGTTT TGCTTCCCAC AGCCACATCT 
CTGCCCATTC CTAGTAAGTC TGCCACAGTT AATCCAGAGT CAAAAACTGA AGCCAAAACC 
CTGGAGGACA TCTTTGAATC AAGCACTTTG TCTGATGGCC AAGCTATTGC AGACCAAAGT 
GAAGTCATAT CAACGTTGGG CTATTTGGAA AGGACACAGA ATGAGGATGA AGCAAAGAAA 
TATGTAAGTC CCTCTTTCCA ACCAGAGTTC TCTTCTGGGG CTGAGGAGGC ACTGACAGAT 
CCTACCCCCT ACGTAAGTAT TGGTACTACC TACCTTACGG CTCAGAGTTT AACAGAGGCA 
CCTGATGTGA TGGAAGGAGC CCGTCTGCCG GATTCCATCG ACACATCCAC AGTTTCAGCT 
TTTTCAGAGC TGCTCTCTCA GACACCATCA TTTCCTCCAC TCAGCATCCA CTTAGGCAGT 
GGAGACTCTG AGCACTCAGA GGACTTGCAG CCAAGTGCTC TGCCAAGTAC AGATGCCAGC 
ACACCCCCAG TATCCTCAGG AGAACTTGCA AACATTGAAG CGACTTTCAA ACCCTCAAGT 
GAAGAAGACT TTTACATAAC TGAGCCTCCA TCCTTACCTC CTGACACAGA GCCTTCAGAA 
GATGAAAGTA AGCCTAAACT ATTAGAACCA ACAGAGGCTT CTGCCACAGA ACTAATTGCT 
CAAGAAGAAA TTGAGATTTT CCAAAATTCT GACAACACAA CCAGTGTCCA GGTTTCTGGG 
GAAGCAGTCA AGGTGTTTCC CAGTATTGAA ACCCCTGAGG CTGAGGCTAT TGTCACAGCT 
GCAAGTGAAA CGAAGTTAGA AGGTGCTACA CTGCGGCCAC ATTCTACTTC TGCTTCTGTG 
ATTCACGGGG TCGAGGCAGG TGTGGTGCCT CAGCCCAGCC CACAGACTTC TGAGCGGCCC 
ACCATCCTTT CTCCTCTGGA AATAAGCCCT GAAACACAGG CAGCTTTGAT CAGAGGGGAG 
GATTCCACAG TAGCGGCCCC CAAACAGCAA GTACCGACGA GGATGCTTGA TTCTAATAAG 
CAGGCAACAC TAAGCACCAC AGAGTTAAAT ACTGAGCTTG CAACACCATC ATTTCCCCTT 
CTGGAAACTT CTAATGAAAC CAGTTTCCTG ATTGGCATTA ATGAAGAGTC AGTGGAAGGC 
ACAGCAGTCT ATTTACCAGG ACCTGATCGT TGCAAAATGA ACCCGTGCCT CAATGGGGGC 
ACCTGTTATC CTACTGAAAC TTCCTACGTG TGCACCTGCG TGCCAGGATA CAGTGGTGAT 
CGGTGTGAAC TTGATTTTGA TGAATGTCAT TCTAATCCTT GTCGCAATGG AGCCACATGT 
ATTGACGGTT TTAATACCTT CAGGTGTCTC TGCCTCCCGA GCTACGTTGG TGCGCTTTGT 
GAGCAAGACA CGGAGACATG TGACTATGGC TGGCACAAAT TTCAAGGGCA GTGCTACAAG 
TACTTTGCCC ATCGACGGAC GTGGGATGCA GCTGAACGGG AATGCCGTCT GCAGGGCGCC 
CATCTCACCA GTATCCTGTC TCACGAGGAA CAAATGTTTG TGAATCGTGT GGGCCATGAT 
TATCAGTGGA TAGGTCTCAA TGACAAGATG TTTGAGCATG ACTTCCGTTG GACTGATGGC 
AGCACACTGC AATATGAGAA CTGGAGGCCG AACCAACCAG ACAGCTTCTT TTCTACTGGA 
GAAGATTGTG TTGTGATTAT ATGGCATGAG AATGGCCAGT GGAATGACGT TCCCTGCAAT 
TACCATCTCA CCTACACCTG CAAGAAAGGA ACAGTTGCTT GCGGCCAGCC CCCTGTTGTA 
GAAAATGCCA AGACCTTTGG AAAGATGAAA CCTCGTTATG AAATCAACTC CTTGATTAGA 
TATCACTGCA AAGATGGTTT CATTCAACGC CACCTTCCAA CTATCCGTTG CCTAGGAAAT 
GGAAGATGGG CTATGCCTAA AATTACCTGC CTGAACCCAT CTGCATACCA AAGGACTTAT 
TCTAAGAAAT ATTTTAAAAA TTCCTCATCA GCAAAGGACA ATTCAATAAA TACATCAAAA 
CATGATCACC GTTGGAGCCG AAGGTGGCAA GAATCAAGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( NM_181035.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_851378.1 Bos taurus versican (VCAN), mRNA.

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CloneID	OBa43820
Clone ID Related Accession (Same CDS sequence)	NM_181035.2
Accession Version	NM_181035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	versican core protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF060456.1. On Jun 3, 2003 this sequence version replaced NM_181035.1. ##Evidence-Data-START## Transcript exon combination :: AF060456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGA 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACCTAAA CAGAATATAT CAGAGGCTAC AACCATCGAA 
CTGAATATGC TTGCAGAAAC TGTCTCACCC ACTTTATTGG AAGAACTACA AGTGGGACTG 
GACAGAATGA CACCAATCGT CCCTTTAATC ACTGAATTAC CTGTCATTAC AACAAAGGTC 
CCTCCTATAG GAAATATTGT CAATTTTGAA CAGAAATCCA CAGTTCAGCC TCTAACTAGT 
ACCCACAGAT CAGCCACAGA ATCACTGCCA CCTGATGGAA GTATGAAGAA GCCTTGGGAT 
ATGGACTATT ACTCACCTTC CGCTTCAGGA CCTCTTGGAG AGCCAGACGT ATCTGAAATT 
AAGGAAGAAG TGCCCCAAAG TACAACTGTC GTTTCCCATC ATGCTCCTGA TTCATGGGAT 
GGTGTGAAGG AAGATCTGCA AATAAAAGAT TCAGTTACAC AAATTGAACA AATAGAAGTG 
GGTCCCTTGG TAACATCTAT GGAAATCTCA AAGCACATTC CTTCTAAGGA ATTCACTGTA 
ACTGTGACAC CATTTGTATC TACAACGATG ACCCTGGAAT CAAAAACTGA AAAGAAAGCA 
ATAAGCACAG TCTCTGAATC AGTGACCACA AGCCACTATG GATTCACCTT GAGAGAAGGT 
GATGGTGAAG ATAGAATATC TACAGTTAGA TCGGGTCAGA GTACCTCGAT CTTTAGCCAA 
ATACCTGAGG TCATTACAGT GTCAAAGACT TCAGAAGACA CCACCCGCGG TCAACTAGAG 
GATGTGGAGT CAGTCTCCGC ATCAACGATT GTCTCACCTG ACAGTGATGG GTCGCCCATG 
GACCACAGGC AAGAGAAACA GACTCATGGC AGGATAACTG AAGGTTTTCT TGGCCAGTAT 
GTGTCCACCA CACCTTTCCC ATCACAGCAT CATACAGAAG TAGAATTGTT TCCTTATTCT 
GGTGATAAAA GATTAGTCGA GGGGACATCC ACAGTTATTT CTCCCACTCC ACGAACAGGA 
AGAGAAAGAA CAGAAACTCT AAGACCAGCG ATGAGAACAG TTACTTACAC CAATGATGAA 
ATACAGGAAA AGATCACCAA AGATTCATCT ATAGAAAAAA TCGAGGAAGA AGGCTTCTCT 
GGAATGAAGT TCCCTACAGC TTCCCCAGAG CAAATTCATC ATACCAAGTA TTCAGTGGGA 
ATGACCAAAT CTTTTGAATC CCCAGCACTG ATGACAACGA CAAAGCCAGG CGTGACACCA 
ACAGAGGCAA CAGATGTGGA GGAAGACTTT ACAACTCCAA GTGGATTAGA AACAGATGGC 
TATCAAGATA CCACAGAGTA TGAGGAGGGT ATTACAACCG TGCATTTGAT CCAGAGCACT 
TTAAATGTGG AGGTGGTTAC TGTATCAAAA TGGTCATTGG ATGAAGATAA TACAACATCA 
AAACCTTTAG GGTCCACAGA ACATGTAGGC TCTCCAAAAT TGCCCCCTGC CTTAATCACC 
ACCACGGGAG TGAGTGGTAA GGATAAAGAA ATGCCAAGTC TTACTGAAGA TGGAAGAGAT 
GAATTCACTC GTATCCCAGG TAGTACTCAA AGGCCACTAG AGGAGTTTAC TGAGGAAGAC 
ACAACAGACC ATGAAAAATT CACAGTTAGA TTTCAGCCAA CTACATCAAT TGCTACCACA 
GAAAAGTCTA CTTTGAGAGA TTCTATAACT GAAGAAAGAG TTCCACCTTT TACAAGCACA 
GAAGTCCGAG TTACTCATGC AACCATAGAG GGAAGTGCTT TGGATGAAGG AGAAGATGTG 
GATGTCTCTA AGCCTTTGTC TACTGTCCCT CAATTTGCAC ACCCTTCAGA TGTGGAAGGG 
TCAACCTTTG TTAATTATAG TAGCACCCAG GAGCCTACTA CTTATGTAGA CACTTCCCAT 
ACCATTCCTC TTCCTGTAAT CCCCAAGACA GAATGGGGAG TGTTGGTACC TTCCATTCCA 
TCAGAAGGTG AAGTTCTAGG CGAACCTTCT CAAGACATAC GTGTCATTAA TCAGACTCAC 
TTTGAAGCTT CTATGTATCC TGAGACTGTA AGAACAACAA CAGAAATTAC ACAAGAAGCA 
ACGCGAGAAG ATTTTCTTTG GAAAGAACAG ACTCCAGAGA AACCAGTTTC TCCTCCCAGT 
TCTACAACTG ACACAGCCAA GGAGACAACA CCACCTTTGG ATGAACAAGA GAGCGATGGG 
TCAGCATATA CTGTCTTTGA AGACAGATCA GTGATGGGTT CTGACAGAGT CTCAGTTTTA 
GTAACAACTC CAATTGGAAA ATTTGAGCAG CATACATCTT TTCCACCTGG TGCTGTCACC 
AAAGCAAAAA CAGATGAAGT GGTAACGCTA ACACCAACCA CAGGGTCAAA GGTAACTTTC 
AGTCCATGGC CTAAACAAAA ATATGAAACA GAAGGTACCA GTCCAAGGGG ATTTGTATCT 
CCTTTTAGCA TTGGTGTTAC CCAGCTTATA GAAGAGACCA CGACTGAGAA AAGAGAGAAA 
ACATCCCTGG ATTATATTGA TTTAGGCTCA GGATTATTCG AAAAGCCCAA GGCCACAGAA 
CTCCCAGAAT TTTCAACAGT TAAAGCCACA GTTCCAAGTG ACACTGCTGC CTTCAGTTCA 
GCAGATAGAC TTCACACACC TTCAGCCTCC ACTGAGAAGC CACCTCTCAT TGACAGAGAA 
CCTGATGAAG AAACAACCAG TGACATGGTA ATCATTGGAG AATCAACATC TCGTGTTCCT 
CCCACTACCC TTGAGGATAT TGTAGCCAAG AAAACAGAAA CAGATATTGA TAGAGAATAT 
TTCACAACTT CAAGTACTTC TACTACACAG CCAACGAGAC CTCCCACTGT GGAAGGCAAA 
GAAGCCTTCG GACCACAGGC ATTTTCTACT CCAGAACCCC CAGCAGGGAC AAAATTCCAC 
CCTGACATAA ATGTTTATAT TATTGAGGTC AGAGAAAACA AGACAGGTCG AATGAGTGAT 
TTCAGTGTAA GTGGTCATCC AATAGATTCA GAATCTAAAG AAGATGAGCC TTGTAGTGAA 
GAAACAGATC CAGAGCACGA TCTAATTGCT GAAATTTTAC CTGAGTTACT TGGTATGTTG 
CACAGTGAAG AGGATGAAGA AGATGAGGAG TGTGCCAATG CCACTGATGT CACGACCACC 
CCATCCGTGC AGTACATAAA TGGGAAGCAT GTAGTTACCA CTGTTCCCAA GGACCCAGAA 
GCAGCAGAAG CTAGGCGTGG CCAGTTCGAA AGTGTTGCAC CTTCTCAAAA TTTTTCAGAC 
AGCAGTGAAA GTGATAGCCA TCAGTTTATA ATCACCCATG CTGGACTGCC CACTGCCATG 
CAACCTAATG AATCTAAAGA AACAACGGAG TCCCTTGAAA TTACGTGGAG ACCCGAGATT 
TACCCTGAAA CAGCAGAGCC TTTCTCAAGT GGCGAGCCTG ATATTTTCCC CACAGCCTCA 
ATCCATGAGG GAGAAGCCAC TGAGGGGCCA GACTCAATCA CAGAGAAAAA TCCTGAACTT 
GATCATCTGG TGCATGAACA TGCTGAATCG GTACCTCTGT TTCCTGAAGA GTCTTCAGGA 
GATGCTGCCA TTGACCAGGA ATCTCAAAAA ATCATCTTTT CAGGGGCTAC CGAAGGAACG 
TTTGGTGAAG AGGCAGAAAA AAGTTCTACC ACACATACTC CAAGTATGGT TGCAAGTTCT 
GTATCAGCAC CTGTCTCAGA GGATGCATCA TTTATCTTAA CAGGCACTCC ACAGTCTGAT 
GAGCCGTTGT CCACAGTAGA AAGCTGGGTG GAAATAACCC CTAGACACAC TGTCGAGTTC 
TCGGGAAGTC CTTCAATTCC AATTCCGGAA GGCTCTGGGG AAGCAGAAGA AGACAAAGAT 
AAAATATTTG CCATGATAAC TGATTTATCA CAGAGAAATA CTACAGACCC CCGCGTTACT 
TCAGACACCA GCAAGATCAT GATCACGGAG AGCCTTGTGG ATGTTCCTAC AACCACTATA 
TATTCAATTT CTGAACAGGT TTCTGCAGTA GTGCCTACCA AATTTGTAAG GGAGACAGAC 
ACTTATGAGT GGGTTTTCAG TCCACCTCTG GAGGAAACAA CAAGGAAAGA GGAGGAGAAG 
GGAACTACAG GTACAGCTTC TACAGTTGAG GTACATTCCC CTACACAGCG ATTAGATCAG 
TTTGTTTCAC CCTCTGAGTT AGAAAGCTCA AGTGAAACTC CACCTGATGA TTCAGCAGCT 
GCTACTAGGA AGAGTTTCAC GTCCCAGATG ACACCCACAC AGTCTGAAAG GGAAACGACA 
AGTTCTACTG TTGTCTTTAA AGAAACAGAA GTTTTAGACA ATCTGGCAGC ACAGACCACA 
GATCCCAGCC TTAGCAGTCA ACCTGGGGTT CTGGAAGTGT CACCTACTGT CCCAGGGAGC 
CCCGTCTCTC TCTTTATGGA GCAAGGCTCC GGAGAAGCTG CTGTTGACCC AGAAACCACC 
ACTGTTTCTT CACTTTCATT AAATATAGAG CCTGAAATTC TAGCTAAAGA GGAGGCAGCT 
GGCGCTTGGT CTCCAAATGT GGAAACTGTA TTCCCTTTTG AGCCAACCGA ACAAGTCTTA 
AGTACTGCAG TGGACAGGGA GGTTGCTGAA ACTATAAGCC AAACATCTAA GGAAAACTTG 
GTTTCAGAGA TCTCAGGAGA GCCAACTCAC AGGGCAGAAA TAAAGGGCTT TTCTACAGAT 
TTTCCTTTGG AGGAAGATTT CAGTGGTGAC TTCAGAGAAT ACTCGACAGT ATCTTATCCC 
ATAACAAAAG AAGAAATAGT GATGATGGAA GGCTCAGGGG ATGCAGCATT TAAGGATACC 
CAGATGTTAC CATCTGTAAC ACCTACCTCG GACCTCAGTA ACCACACTGC TGACTCGGAA 
GAACCTGGCA GCACCTTGGT CAGCACCTCA GCCTTCCCTT GGGAAGAATT CACAGCCTCT 
GCTGAGGGCT CAGGTGAGCC CTTGCTCTCA GTAAGCAGCT CTGTTGACCA AGTGTTTCCC 
AGTGCCGCGG GAAAAGCCTC TGGAACAGAT TCCCCATTTA TTGACCAACG ATTGGGAGAA 
GAAGGTGCTA TCAATGAAAC TGATCAGAGA TCCACCATTT TGCCAACAGC AGAAGCTGAG 
AGTACTAAAG CTTCAACTGA AGAGGGGGAG GTGAAGGAGA ATCACACAGT TTCAATGGAC 
TTTCCCCCAA CTGTGGAGCC AGACGAATTA TGGCCTAGAC AAGAAGTCAA CCCTGTAAGG 
CAAGGAAATG GAAGTGAAAT AGTATCAGAG GAAAAGACTC AAGAGCAAGA ATCTTTTGAA 
CCCCTTCAAA GCTCTGTTGC ACCAGAACAA ACAACTTTTG ATTCACAGAC ATTTCCTGAA 
CCTGGACTCC AAACCACAGG TTATTTCACA CTAACGACAA AGAAAACTTA CAGTACTGAT 
GAGAGAATGG AGGAGGAAGT CATTTCCTTA GCTGACGTGT CCACTCCAAC TCTAGATTCA 
AAGGGCTTGG TATTGTACAC TACTCTCCCT GAAGTTACTG AAAAATCCCA TTTTTTCTTA 
GCTACTGCCT CAGTGACTGA ATCAGTACCA GCTGAAAGTG TAATTGCAGG TTCAACCATC 
AAAGAGGAAG AGAGTATAAA ACCCTTTCCC AAAGTTACGA GCCCGATAAT TAAAGAGTCA 
GATACAGATC TTATATTCTC TGGACTGGGA TCTGGGGAAG AAGTTTTGCC TACTCTAGGA 
TCAGTGAATT TTACTGAAAT AGAACAAGTC CTTAGCACAT TATATCCCCT GACTTCTCAA 
GTACAAAGTT TAGAAGCCAG CATCTTAAAT GATACAAGTG GAGACTATGA GGGAATGGAA 
AATGTAGCAA ATGAAATGAG ACCACTCATT TCCAAAACAG ACAGCATTTT TGAAGATGGT 
GAGACAGCAT CCAGCACAAC ACTACCAGAA ATTTTAAGTG ACGCCAGAAC AGAAGGGCCC 
TTCACGGCAC CTCTCACTTT TTCCACAGGC CCCGGACAGC CTCAAAATCA GACGCACCGT 
AGGGCAGAAG AAATCCAGAC CAGTAGACCA CAGCCTCTGA CTGATCAAGT CTCTAGCGAG 
AATTCTGTGA CAGCAGAAAC TAAAGAAACA GCAACCCCTG CCACTGATTT TCTGGCTAGA 
ACATATGATC TTGAAATGGC CAAAGGATTT GTTACACCAA CACCGAAACC ATCTGACCTG 
TTTTATGAAC ATTCTGGAGA GGGTTCTGGG GAATTGGATG CCGTTGGTGC AGAAGTCCAC 
GCTTCTGGAA TGACTCAGGC AACTAGGCAA GGAAGCACCA CATTTGTTTC TGATAGATCC 
CTGGAAAAAC ATCCCAAGGT TCCAAGTGTC GAAGCTGTTA CTGTTAATGG ATTCCCGACA 
GTTTCAATGG TGCTGCCGCT TCATCCTGAG CAGAGAGAAG GCTCTCCTGA AGCAACCGGC 
ACACCGGCAA GCACAGCATC ATATGAGAAG GCCACAGAAG GTGCGGCAGA TAGTTTCCAA 
GACCATTTCT GGGGATTCAA GGATTCCACG CTGAAACCCG ACAAAAGAAA AGCCACTGAA 
AGTATTATTA TAGATCTGGA CAAAGAGGAC AAGGATTTAA TATTGACAAT GACGGAGAGT 
ACCATCCTTG AAATTATACC TGAGCTCACA TCAGATAAAA ATACTGTCAT AGATATTGAT 
CACACTAAAC CAATATATGA AGACATCCTT GGAATGCAGA CGGACTTGGA TCCAGAAGTA 
CCCTCAGGAC CGCCTGACAG TAGTGAAGAG AGCACTCAAG TTCAAGAGAA GTATGAGGCA 
GCTGTTAACC TGTCTTCAAC TGAGGAAAAC TTTGAGGCTT CTGGTGATAT TCTGCTGGCT 
AATTACACTC AGGCAACACC TGAATCGAAG GCTCCTGAAG ACAGAAATCC ATTAGATCAC 
ACAGACTTTA TCTTCACGAC TGGGATCCCC ATTCTTAGCT CAGAGACAGA ATTAGACGTT 
TTGCTTCCCA CAGCCACATC TCTGCCCATT CCTAGTAAGT CTGCCACAGT TAATCCAGAG 
TCAAAAACTG AAGCCAAAAC CCTGGAGGAC ATCTTTGAAT CAAGCACTTT GTCTGATGGC 
CAAGCTATTG CAGACCAAAG TGAAGTCATA TCAACGTTGG GCTATTTGGA AAGGACACAG 
AATGAGGATG AAGCAAAGAA ATATGTAAGT CCCTCTTTCC AACCAGAGTT CTCTTCTGGG 
GCTGAGGAGG CACTGACAGA TCCTACCCCC TACGTAAGTA TTGGTACTAC CTACCTTACG 
GCTCAGAGTT TAACAGAGGC ACCTGATGTG ATGGAAGGAG CCCGTCTGCC GGATTCCATC 
GACACATCCA CAGTTTCAGC TTTTTCAGAG CTGCTCTCTC AGACACCATC ATTTCCTCCA 
CTCAGCATCC ACTTAGGCAG TGGAGACTCT GAGCACTCAG AGGACTTGCA GCCAAGTGCT 
CTGCCAAGTA CAGATGCCAG CACACCCCCA GTATCCTCAG GAGAACTTGC AAACATTGAA 
GCGACTTTCA AACCCTCAAG TGAAGAAGAC TTTTACATAA CTGAGCCTCC ATCCTTACCT 
CCTGACACAG AGCCTTCAGA AGATGAAAGT AAGCCTAAAC TATTAGAACC AACAGAGGCT 
TCTGCCACAG AACTAATTGC TCAAGAAGAA ATTGAGATTT TCCAAAATTC TGACAACACA 
ACCAGTGTCC AGGTTTCTGG GGAAGCAGTC AAGGTGTTTC CCAGTATTGA AACCCCTGAG 
GCTGAGGCTA TTGTCACAGC TGCAAGTGAA ACGAAGTTAG AAGGTGCTAC ACTGCGGCCA 
CATTCTACTT CTGCTTCTGT GATTCACGGG GTCGAGGCAG GTGTGGTGCC TCAGCCCAGC 
CCACAGACTT CTGAGCGGCC CACCATCCTT TCTCCTCTGG AAATAAGCCC TGAAACACAG 
GCAGCTTTGA TCAGAGGGGA GGATTCCACA GTAGCGGCCC CCAAACAGCA AGTACCGACG 
AGGATGCTTG ATTCTAATAA GCAGGCAACA CTAAGCACCA CAGAGTTAAA TACTGAGCTT 
GCAACACCAT CATTTCCCCT TCTGGAAACT TCTAATGAAA CCAGTTTCCT GATTGGCATT 
AATGAAGAGT CAGTGGAAGG CACAGCAGTC TATTTACCAG GACCTGATCG TTGCAAAATG 
AACCCGTGCC TCAATGGGGG CACCTGTTAT CCTACTGAAA CTTCCTACGT GTGCACCTGC 
GTGCCAGGAT ACAGTGGTGA TCGGTGTGAA CTTGATTTTG ATGAATGTCA TTCTAATCCT 
TGTCGCAATG GAGCCACATG TATTGACGGT TTTAATACCT TCAGGTGTCT CTGCCTCCCG 
AGCTACGTTG GTGCGCTTTG TGAGCAAGAC ACGGAGACAT GTGACTATGG CTGGCACAAA 
TTTCAAGGGC AGTGCTACAA GTACTTTGCC CATCGACGGA CGTGGGATGC AGCTGAACGG 
GAATGCCGTC TGCAGGGCGC CCATCTCACC AGTATCCTGT CTCACGAGGA ACAAATGTTT 
GTGAATCGTG TGGGCCATGA TTATCAGTGG ATAGGTCTCA ATGACAAGAT GTTTGAGCAT 
GACTTCCGTT GGACTGATGG CAGCACACTG CAATATGAGA ACTGGAGGCC GAACCAACCA 
GACAGCTTCT TTTCTACTGG AGAAGATTGT GTTGTGATTA TATGGCATGA GAATGGCCAG 
TGGAATGACG TTCCCTGCAA TTACCATCTC ACCTACACCT GCAAGAAAGG AACAGTTGCT 
TGCGGCCAGC CCCCTGTTGT AGAAAATGCC AAGACCTTTG GAAAGATGAA ACCTCGTTAT 
GAAATCAACT CCTTGATTAG ATATCACTGC AAAGATGGTT TCATTCAACG CCACCTTCCA 
ACTATCCGTT GCCTAGGAAA TGGAAGATGG GCTATGCCTA AAATTACCTG CCTGAACCCA 
TCTGCATACC AAAGGACTTA TTCTAAGAAA TATTTTAAAA ATTCCTCATC AGCAAAGGAC 
AATTCAATAA ATACATCAAA ACATGATCAC CGTTGGAGCC GAAGGTGGCA AGAATCAAGG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851378.1
CDS	88..10233
Translation	MLINIKSILWMCSTLIAAHALQKVNMEKSPPVKGSLSGKVNLPCHFSTMPTLPPSYNTTSEFLRIKWSKIELDKTGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEDVGDASLTMVKLLASDAGRYRCDVMYGIEDTQDTVSLTVEGVVFHYRAATSRYTLNFEMAQKACVDIGAVIATPEQLHAAYEDGFEQCDAGWLSDQTVRYPIRVPREGCYGDMMGKEGVRTYGFRAPHETYDVYCYVDHLDGDVFHITAPNKFTFEEAGEECKTQDARLATVGELQAAWRNGFDRCDYGWLLDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPTPDSRFDAYCFKPKQNISEATTIELNMLAETVSPTLLEELQVGLDRMTPIVPLITELPVITTKVPPIGNIVNFEQKSTVQPLTSTHRSATESLPPDGSMKKPWDMDYYSPSASGPLGEPDVSEIKEEVPQSTTVVSHHAPDSWDGVKEDLQIKDSVTQIEQIEVGPLVTSMEISKHIPSKEFTVTVTPFVSTTMTLESKTEKKAISTVSESVTTSHYGFTLREGDGEDRISTVRSGQSTSIFSQIPEVITVSKTSEDTTRGQLEDVESVSASTIVSPDSDGSPMDHRQEKQTHGRITEGFLGQYVSTTPFPSQHHTEVELFPYSGDKRLVEGTSTVISPTPRTGRERTETLRPAMRTVTYTNDEIQEKITKDSSIEKIEEEGFSGMKFPTASPEQIHHTKYSVGMTKSFESPALMTTTKPGVTPTEATDVEEDFTTPSGLETDGYQDTTEYEEGITTVHLIQSTLNVEVVTVSKWSLDEDNTTSKPLGSTEHVGSPKLPPALITTTGVSGKDKEMPSLTEDGRDEFTRIPGSTQRPLEEFTEEDTTDHEKFTVRFQPTTSIATTEKSTLRDSITEERVPPFTSTEVRVTHATIEGSALDEGEDVDVSKPLSTVPQFAHPSDVEGSTFVNYSSTQEPTTYVDTSHTIPLPVIPKTEWGVLVPSIPSEGEVLGEPSQDIRVINQTHFEASMYPETVRTTTEITQEATREDFLWKEQTPEKPVSPPSSTTDTAKETTPPLDEQESDGSAYTVFEDRSVMGSDRVSVLVTTPIGKFEQHTSFPPGAVTKAKTDEVVTLTPTTGSKVTFSPWPKQKYETEGTSPRGFVSPFSIGVTQLIEETTTEKREKTSLDYIDLGSGLFEKPKATELPEFSTVKATVPSDTAAFSSADRLHTPSASTEKPPLIDREPDEETTSDMVIIGESTSRVPPTTLEDIVAKKTETDIDREYFTTSSTSTTQPTRPPTVEGKEAFGPQAFSTPEPPAGTKFHPDINVYIIEVRENKTGRMSDFSVSGHPIDSESKEDEPCSEETDPEHDLIAEILPELLGMLHSEEDEEDEECANATDVTTTPSVQYINGKHVVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDSHQFIITHAGLPTAMQPNESKETTESLEITWRPEIYPETAEPFSSGEPDIFPTASIHEGEATEGPDSITEKNPELDHLVHEHAESVPLFPEESSGDAAIDQESQKIIFSGATEGTFGEEAEKSSTTHTPSMVASSVSAPVSEDASFILTGTPQSDEPLSTVESWVEITPRHTVEFSGSPSIPIPEGSGEAEEDKDKIFAMITDLSQRNTTDPRVTSDTSKIMITESLVDVPTTTIYSISEQVSAVVPTKFVRETDTYEWVFSPPLEETTRKEEEKGTTGTASTVEVHSPTQRLDQFVSPSELESSSETPPDDSAAATRKSFTSQMTPTQSERETTSSTVVFKETEVLDNLAAQTTDPSLSSQPGVLEVSPTVPGSPVSLFMEQGSGEAAVDPETTTVSSLSLNIEPEILAKEEAAGAWSPNVETVFPFEPTEQVLSTAVDREVAETISQTSKENLVSEISGEPTHRAEIKGFSTDFPLEEDFSGDFREYSTVSYPITKEEIVMMEGSGDAAFKDTQMLPSVTPTSDLSNHTADSEEPGSTLVSTSAFPWEEFTASAEGSGEPLLSVSSSVDQVFPSAAGKASGTDSPFIDQRLGEEGAINETDQRSTILPTAEAESTKASTEEGEVKENHTVSMDFPPTVEPDELWPRQEVNPVRQGNGSEIVSEEKTQEQESFEPLQSSVAPEQTTFDSQTFPEPGLQTTGYFTLTTKKTYSTDERMEEEVISLADVSTPTLDSKGLVLYTTLPEVTEKSHFFLATASVTESVPAESVIAGSTIKEEESIKPFPKVTSPIIKESDTDLIFSGLGSGEEVLPTLGSVNFTEIEQVLSTLYPLTSQVQSLEASILNDTSGDYEGMENVANEMRPLISKTDSIFEDGETASSTTLPEILSDARTEGPFTAPLTFSTGPGQPQNQTHRRAEEIQTSRPQPLTDQVSSENSVTAETKETATPATDFLARTYDLEMAKGFVTPTPKPSDLFYEHSGEGSGELDAVGAEVHASGMTQATRQGSTTFVSDRSLEKHPKVPSVEAVTVNGFPTVSMVLPLHPEQREGSPEATGTPASTASYEKATEGAADSFQDHFWGFKDSTLKPDKRKATESIIIDLDKEDKDLILTMTESTILEIIPELTSDKNTVIDIDHTKPIYEDILGMQTDLDPEVPSGPPDSSEESTQVQEKYEAAVNLSSTEENFEASGDILLANYTQATPESKAPEDRNPLDHTDFIFTTGIPILSSETELDVLLPTATSLPIPSKSATVNPESKTEAKTLEDIFESSTLSDGQAIADQSEVISTLGYLERTQNEDEAKKYVSPSFQPEFSSGAEEALTDPTPYVSIGTTYLTAQSLTEAPDVMEGARLPDSIDTSTVSAFSELLSQTPSFPPLSIHLGSGDSEHSEDLQPSALPSTDASTPPVSSGELANIEATFKPSSEEDFYITEPPSLPPDTEPSEDESKPKLLEPTEASATELIAQEEIEIFQNSDNTTSVQVSGEAVKVFPSIETPEAEAIVTAASETKLEGATLRPHSTSASVIHGVEAGVVPQPSPQTSERPTILSPLEISPETQAALIRGEDSTVAAPKQQVPTRMLDSNKQATLSTTELNTELATPSFPLLETSNETSFLIGINEESVEGTAVYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDRCELDFDECHSNPCRNGATCIDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSTGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCLNPSAYQRTYSKKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_181035.2
Organism	Bos taurus(cattle)
Definition	Bos taurus versican (VCAN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181035.2

ORF Insert Sequence:

ATGCTGATAA ATATAAAGAG CATCTTATGG ATGTGCTCCA CCTTAATAGC AGCCCATGCA 
CTACAGAAAG TCAACATGGA AAAAAGCCCA CCTGTTAAGG GCTCCCTCTC TGGAAAAGTC 
AACCTACCTT GTCATTTCTC AACCATGCCT ACCTTACCAC CCAGTTATAA CACCACCAGT 
GAATTTCTGC GAATCAAATG GTCTAAGATT GAATTGGACA AGACGGGAAA AGATTTAAAG 
GAGACTACTG TTCTCGTGGC CCAAAATGGG AATATCAAGA TCGGTCAAGA CTACAAAGGG 
AGAGTGTCGG TGCCTACACA TCCCGAGGAT GTGGGCGATG CCTCACTCAC CATGGTCAAA 
CTGCTGGCCA GTGATGCAGG CCGCTACCGC TGTGACGTCA TGTACGGGAT TGAAGACACA 
CAGGACACGG TGTCACTGAC CGTGGAGGGG GTTGTGTTTC ACTACAGGGC GGCAACCAGC 
AGGTACACCC TGAACTTTGA GATGGCACAG AAGGCTTGTG TGGACATCGG GGCCGTCATA 
GCCACCCCAG AGCAGCTGCA TGCCGCCTAT GAAGATGGTT TTGAGCAGTG TGATGCAGGA 
TGGCTGTCAG ATCAGACTGT TAGGTATCCC ATCCGGGTTC CCCGAGAAGG CTGCTATGGA 
GACATGATGG GGAAGGAAGG AGTCCGGACC TACGGATTCC GTGCTCCTCA TGAAACTTAC 
GATGTGTATT GTTACGTGGA CCATCTGGAT GGTGATGTGT TCCACATCAC TGCTCCCAAC 
AAGTTCACCT TTGAGGAAGC TGGAGAAGAG TGTAAAACCC AGGACGCCCG CCTGGCGACC 
GTGGGGGAGC TCCAAGCAGC GTGGAGGAAC GGCTTCGACC GGTGTGATTA CGGGTGGCTG 
TTGGACGCCA GCGTTCGCCA CCCTGTGACT GTGGCCAGGG CCCAGTGTGG AGGTGGTTTA 
CTTGGGGTGA GAACCCTGTA TCGTTTTGAG AACCAGACAG GCTTCCCTAC CCCTGATAGC 
AGATTTGATG CCTACTGCTT TAAACCTAAA CAGAATATAT CAGAGGCTAC AACCATCGAA 
CTGAATATGC TTGCAGAAAC TGTCTCACCC ACTTTATTGG AAGAACTACA AGTGGGACTG 
GACAGAATGA CACCAATCGT CCCTTTAATC ACTGAATTAC CTGTCATTAC AACAAAGGTC 
CCTCCTATAG GAAATATTGT CAATTTTGAA CAGAAATCCA CAGTTCAGCC TCTAACTAGT 
ACCCACAGAT CAGCCACAGA ATCACTGCCA CCTGATGGAA GTATGAAGAA GCCTTGGGAT 
ATGGACTATT ACTCACCTTC CGCTTCAGGA CCTCTTGGAG AGCCAGACGT ATCTGAAATT 
AAGGAAGAAG TGCCCCAAAG TACAACTGTC GTTTCCCATC ATGCTCCTGA TTCATGGGAT 
GGTGTGAAGG AAGATCTGCA AATAAAAGAT TCAGTTACAC AAATTGAACA AATAGAAGTG 
GGTCCCTTGG TAACATCTAT GGAAATCTCA AAGCACATTC CTTCTAAGGA ATTCACTGTA 
ACTGTGACAC CATTTGTATC TACAACGATG ACCCTGGAAT CAAAAACTGA AAAGAAAGCA 
ATAAGCACAG TCTCTGAATC AGTGACCACA AGCCACTATG GATTCACCTT GAGAGAAGGT 
GATGGTGAAG ATAGAATATC TACAGTTAGA TCGGGTCAGA GTACCTCGAT CTTTAGCCAA 
ATACCTGAGG TCATTACAGT GTCAAAGACT TCAGAAGACA CCACCCGCGG TCAACTAGAG 
GATGTGGAGT CAGTCTCCGC ATCAACGATT GTCTCACCTG ACAGTGATGG GTCGCCCATG 
GACCACAGGC AAGAGAAACA GACTCATGGC AGGATAACTG AAGGTTTTCT TGGCCAGTAT 
GTGTCCACCA CACCTTTCCC ATCACAGCAT CATACAGAAG TAGAATTGTT TCCTTATTCT 
GGTGATAAAA GATTAGTCGA GGGGACATCC ACAGTTATTT CTCCCACTCC ACGAACAGGA 
AGAGAAAGAA CAGAAACTCT AAGACCAGCG ATGAGAACAG TTACTTACAC CAATGATGAA 
ATACAGGAAA AGATCACCAA AGATTCATCT ATAGAAAAAA TCGAGGAAGA AGGCTTCTCT 
GGAATGAAGT TCCCTACAGC TTCCCCAGAG CAAATTCATC ATACCAAGTA TTCAGTGGGA 
ATGACCAAAT CTTTTGAATC CCCAGCACTG ATGACAACGA CAAAGCCAGG CGTGACACCA 
ACAGAGGCAA CAGATGTGGA GGAAGACTTT ACAACTCCAA GTGGATTAGA AACAGATGGC 
TATCAAGATA CCACAGAGTA TGAGGAGGGT ATTACAACCG TGCATTTGAT CCAGAGCACT 
TTAAATGTGG AGGTGGTTAC TGTATCAAAA TGGTCATTGG ATGAAGATAA TACAACATCA 
AAACCTTTAG GGTCCACAGA ACATGTAGGC TCTCCAAAAT TGCCCCCTGC CTTAATCACC 
ACCACGGGAG TGAGTGGTAA GGATAAAGAA ATGCCAAGTC TTACTGAAGA TGGAAGAGAT 
GAATTCACTC GTATCCCAGG TAGTACTCAA AGGCCACTAG AGGAGTTTAC TGAGGAAGAC 
ACAACAGACC ATGAAAAATT CACAGTTAGA TTTCAGCCAA CTACATCAAT TGCTACCACA 
GAAAAGTCTA CTTTGAGAGA TTCTATAACT GAAGAAAGAG TTCCACCTTT TACAAGCACA 
GAAGTCCGAG TTACTCATGC AACCATAGAG GGAAGTGCTT TGGATGAAGG AGAAGATGTG 
GATGTCTCTA AGCCTTTGTC TACTGTCCCT CAATTTGCAC ACCCTTCAGA TGTGGAAGGG 
TCAACCTTTG TTAATTATAG TAGCACCCAG GAGCCTACTA CTTATGTAGA CACTTCCCAT 
ACCATTCCTC TTCCTGTAAT CCCCAAGACA GAATGGGGAG TGTTGGTACC TTCCATTCCA 
TCAGAAGGTG AAGTTCTAGG CGAACCTTCT CAAGACATAC GTGTCATTAA TCAGACTCAC 
TTTGAAGCTT CTATGTATCC TGAGACTGTA AGAACAACAA CAGAAATTAC ACAAGAAGCA 
ACGCGAGAAG ATTTTCTTTG GAAAGAACAG ACTCCAGAGA AACCAGTTTC TCCTCCCAGT 
TCTACAACTG ACACAGCCAA GGAGACAACA CCACCTTTGG ATGAACAAGA GAGCGATGGG 
TCAGCATATA CTGTCTTTGA AGACAGATCA GTGATGGGTT CTGACAGAGT CTCAGTTTTA 
GTAACAACTC CAATTGGAAA ATTTGAGCAG CATACATCTT TTCCACCTGG TGCTGTCACC 
AAAGCAAAAA CAGATGAAGT GGTAACGCTA ACACCAACCA CAGGGTCAAA GGTAACTTTC 
AGTCCATGGC CTAAACAAAA ATATGAAACA GAAGGTACCA GTCCAAGGGG ATTTGTATCT 
CCTTTTAGCA TTGGTGTTAC CCAGCTTATA GAAGAGACCA CGACTGAGAA AAGAGAGAAA 
ACATCCCTGG ATTATATTGA TTTAGGCTCA GGATTATTCG AAAAGCCCAA GGCCACAGAA 
CTCCCAGAAT TTTCAACAGT TAAAGCCACA GTTCCAAGTG ACACTGCTGC CTTCAGTTCA 
GCAGATAGAC TTCACACACC TTCAGCCTCC ACTGAGAAGC CACCTCTCAT TGACAGAGAA 
CCTGATGAAG AAACAACCAG TGACATGGTA ATCATTGGAG AATCAACATC TCGTGTTCCT 
CCCACTACCC TTGAGGATAT TGTAGCCAAG AAAACAGAAA CAGATATTGA TAGAGAATAT 
TTCACAACTT CAAGTACTTC TACTACACAG CCAACGAGAC CTCCCACTGT GGAAGGCAAA 
GAAGCCTTCG GACCACAGGC ATTTTCTACT CCAGAACCCC CAGCAGGGAC AAAATTCCAC 
CCTGACATAA ATGTTTATAT TATTGAGGTC AGAGAAAACA AGACAGGTCG AATGAGTGAT 
TTCAGTGTAA GTGGTCATCC AATAGATTCA GAATCTAAAG AAGATGAGCC TTGTAGTGAA 
GAAACAGATC CAGAGCACGA TCTAATTGCT GAAATTTTAC CTGAGTTACT TGGTATGTTG 
CACAGTGAAG AGGATGAAGA AGATGAGGAG TGTGCCAATG CCACTGATGT CACGACCACC 
CCATCCGTGC AGTACATAAA TGGGAAGCAT GTAGTTACCA CTGTTCCCAA GGACCCAGAA 
GCAGCAGAAG CTAGGCGTGG CCAGTTCGAA AGTGTTGCAC CTTCTCAAAA TTTTTCAGAC 
AGCAGTGAAA GTGATAGCCA TCAGTTTATA ATCACCCATG CTGGACTGCC CACTGCCATG 
CAACCTAATG AATCTAAAGA AACAACGGAG TCCCTTGAAA TTACGTGGAG ACCCGAGATT 
TACCCTGAAA CAGCAGAGCC TTTCTCAAGT GGCGAGCCTG ATATTTTCCC CACAGCCTCA 
ATCCATGAGG GAGAAGCCAC TGAGGGGCCA GACTCAATCA CAGAGAAAAA TCCTGAACTT 
GATCATCTGG TGCATGAACA TGCTGAATCG GTACCTCTGT TTCCTGAAGA GTCTTCAGGA 
GATGCTGCCA TTGACCAGGA ATCTCAAAAA ATCATCTTTT CAGGGGCTAC CGAAGGAACG 
TTTGGTGAAG AGGCAGAAAA AAGTTCTACC ACACATACTC CAAGTATGGT TGCAAGTTCT 
GTATCAGCAC CTGTCTCAGA GGATGCATCA TTTATCTTAA CAGGCACTCC ACAGTCTGAT 
GAGCCGTTGT CCACAGTAGA AAGCTGGGTG GAAATAACCC CTAGACACAC TGTCGAGTTC 
TCGGGAAGTC CTTCAATTCC AATTCCGGAA GGCTCTGGGG AAGCAGAAGA AGACAAAGAT 
AAAATATTTG CCATGATAAC TGATTTATCA CAGAGAAATA CTACAGACCC CCGCGTTACT 
TCAGACACCA GCAAGATCAT GATCACGGAG AGCCTTGTGG ATGTTCCTAC AACCACTATA 
TATTCAATTT CTGAACAGGT TTCTGCAGTA GTGCCTACCA AATTTGTAAG GGAGACAGAC 
ACTTATGAGT GGGTTTTCAG TCCACCTCTG GAGGAAACAA CAAGGAAAGA GGAGGAGAAG 
GGAACTACAG GTACAGCTTC TACAGTTGAG GTACATTCCC CTACACAGCG ATTAGATCAG 
TTTGTTTCAC CCTCTGAGTT AGAAAGCTCA AGTGAAACTC CACCTGATGA TTCAGCAGCT 
GCTACTAGGA AGAGTTTCAC GTCCCAGATG ACACCCACAC AGTCTGAAAG GGAAACGACA 
AGTTCTACTG TTGTCTTTAA AGAAACAGAA GTTTTAGACA ATCTGGCAGC ACAGACCACA 
GATCCCAGCC TTAGCAGTCA ACCTGGGGTT CTGGAAGTGT CACCTACTGT CCCAGGGAGC 
CCCGTCTCTC TCTTTATGGA GCAAGGCTCC GGAGAAGCTG CTGTTGACCC AGAAACCACC 
ACTGTTTCTT CACTTTCATT AAATATAGAG CCTGAAATTC TAGCTAAAGA GGAGGCAGCT 
GGCGCTTGGT CTCCAAATGT GGAAACTGTA TTCCCTTTTG AGCCAACCGA ACAAGTCTTA 
AGTACTGCAG TGGACAGGGA GGTTGCTGAA ACTATAAGCC AAACATCTAA GGAAAACTTG 
GTTTCAGAGA TCTCAGGAGA GCCAACTCAC AGGGCAGAAA TAAAGGGCTT TTCTACAGAT 
TTTCCTTTGG AGGAAGATTT CAGTGGTGAC TTCAGAGAAT ACTCGACAGT ATCTTATCCC 
ATAACAAAAG AAGAAATAGT GATGATGGAA GGCTCAGGGG ATGCAGCATT TAAGGATACC 
CAGATGTTAC CATCTGTAAC ACCTACCTCG GACCTCAGTA ACCACACTGC TGACTCGGAA 
GAACCTGGCA GCACCTTGGT CAGCACCTCA GCCTTCCCTT GGGAAGAATT CACAGCCTCT 
GCTGAGGGCT CAGGTGAGCC CTTGCTCTCA GTAAGCAGCT CTGTTGACCA AGTGTTTCCC 
AGTGCCGCGG GAAAAGCCTC TGGAACAGAT TCCCCATTTA TTGACCAACG ATTGGGAGAA 
GAAGGTGCTA TCAATGAAAC TGATCAGAGA TCCACCATTT TGCCAACAGC AGAAGCTGAG 
AGTACTAAAG CTTCAACTGA AGAGGGGGAG GTGAAGGAGA ATCACACAGT TTCAATGGAC 
TTTCCCCCAA CTGTGGAGCC AGACGAATTA TGGCCTAGAC AAGAAGTCAA CCCTGTAAGG 
CAAGGAAATG GAAGTGAAAT AGTATCAGAG GAAAAGACTC AAGAGCAAGA ATCTTTTGAA 
CCCCTTCAAA GCTCTGTTGC ACCAGAACAA ACAACTTTTG ATTCACAGAC ATTTCCTGAA 
CCTGGACTCC AAACCACAGG TTATTTCACA CTAACGACAA AGAAAACTTA CAGTACTGAT 
GAGAGAATGG AGGAGGAAGT CATTTCCTTA GCTGACGTGT CCACTCCAAC TCTAGATTCA 
AAGGGCTTGG TATTGTACAC TACTCTCCCT GAAGTTACTG AAAAATCCCA TTTTTTCTTA 
GCTACTGCCT CAGTGACTGA ATCAGTACCA GCTGAAAGTG TAATTGCAGG TTCAACCATC 
AAAGAGGAAG AGAGTATAAA ACCCTTTCCC AAAGTTACGA GCCCGATAAT TAAAGAGTCA 
GATACAGATC TTATATTCTC TGGACTGGGA TCTGGGGAAG AAGTTTTGCC TACTCTAGGA 
TCAGTGAATT TTACTGAAAT AGAACAAGTC CTTAGCACAT TATATCCCCT GACTTCTCAA 
GTACAAAGTT TAGAAGCCAG CATCTTAAAT GATACAAGTG GAGACTATGA GGGAATGGAA 
AATGTAGCAA ATGAAATGAG ACCACTCATT TCCAAAACAG ACAGCATTTT TGAAGATGGT 
GAGACAGCAT CCAGCACAAC ACTACCAGAA ATTTTAAGTG ACGCCAGAAC AGAAGGGCCC 
TTCACGGCAC CTCTCACTTT TTCCACAGGC CCCGGACAGC CTCAAAATCA GACGCACCGT 
AGGGCAGAAG AAATCCAGAC CAGTAGACCA CAGCCTCTGA CTGATCAAGT CTCTAGCGAG 
AATTCTGTGA CAGCAGAAAC TAAAGAAACA GCAACCCCTG CCACTGATTT TCTGGCTAGA 
ACATATGATC TTGAAATGGC CAAAGGATTT GTTACACCAA CACCGAAACC ATCTGACCTG 
TTTTATGAAC ATTCTGGAGA GGGTTCTGGG GAATTGGATG CCGTTGGTGC AGAAGTCCAC 
GCTTCTGGAA TGACTCAGGC AACTAGGCAA GGAAGCACCA CATTTGTTTC TGATAGATCC 
CTGGAAAAAC ATCCCAAGGT TCCAAGTGTC GAAGCTGTTA CTGTTAATGG ATTCCCGACA 
GTTTCAATGG TGCTGCCGCT TCATCCTGAG CAGAGAGAAG GCTCTCCTGA AGCAACCGGC 
ACACCGGCAA GCACAGCATC ATATGAGAAG GCCACAGAAG GTGCGGCAGA TAGTTTCCAA 
GACCATTTCT GGGGATTCAA GGATTCCACG CTGAAACCCG ACAAAAGAAA AGCCACTGAA 
AGTATTATTA TAGATCTGGA CAAAGAGGAC AAGGATTTAA TATTGACAAT GACGGAGAGT 
ACCATCCTTG AAATTATACC TGAGCTCACA TCAGATAAAA ATACTGTCAT AGATATTGAT 
CACACTAAAC CAATATATGA AGACATCCTT GGAATGCAGA CGGACTTGGA TCCAGAAGTA 
CCCTCAGGAC CGCCTGACAG TAGTGAAGAG AGCACTCAAG TTCAAGAGAA GTATGAGGCA 
GCTGTTAACC TGTCTTCAAC TGAGGAAAAC TTTGAGGCTT CTGGTGATAT TCTGCTGGCT 
AATTACACTC AGGCAACACC TGAATCGAAG GCTCCTGAAG ACAGAAATCC ATTAGATCAC 
ACAGACTTTA TCTTCACGAC TGGGATCCCC ATTCTTAGCT CAGAGACAGA ATTAGACGTT 
TTGCTTCCCA CAGCCACATC TCTGCCCATT CCTAGTAAGT CTGCCACAGT TAATCCAGAG 
TCAAAAACTG AAGCCAAAAC CCTGGAGGAC ATCTTTGAAT CAAGCACTTT GTCTGATGGC 
CAAGCTATTG CAGACCAAAG TGAAGTCATA TCAACGTTGG GCTATTTGGA AAGGACACAG 
AATGAGGATG AAGCAAAGAA ATATGTAAGT CCCTCTTTCC AACCAGAGTT CTCTTCTGGG 
GCTGAGGAGG CACTGACAGA TCCTACCCCC TACGTAAGTA TTGGTACTAC CTACCTTACG 
GCTCAGAGTT TAACAGAGGC ACCTGATGTG ATGGAAGGAG CCCGTCTGCC GGATTCCATC 
GACACATCCA CAGTTTCAGC TTTTTCAGAG CTGCTCTCTC AGACACCATC ATTTCCTCCA 
CTCAGCATCC ACTTAGGCAG TGGAGACTCT GAGCACTCAG AGGACTTGCA GCCAAGTGCT 
CTGCCAAGTA CAGATGCCAG CACACCCCCA GTATCCTCAG GAGAACTTGC AAACATTGAA 
GCGACTTTCA AACCCTCAAG TGAAGAAGAC TTTTACATAA CTGAGCCTCC ATCCTTACCT 
CCTGACACAG AGCCTTCAGA AGATGAAAGT AAGCCTAAAC TATTAGAACC AACAGAGGCT 
TCTGCCACAG AACTAATTGC TCAAGAAGAA ATTGAGATTT TCCAAAATTC TGACAACACA 
ACCAGTGTCC AGGTTTCTGG GGAAGCAGTC AAGGTGTTTC CCAGTATTGA AACCCCTGAG 
GCTGAGGCTA TTGTCACAGC TGCAAGTGAA ACGAAGTTAG AAGGTGCTAC ACTGCGGCCA 
CATTCTACTT CTGCTTCTGT GATTCACGGG GTCGAGGCAG GTGTGGTGCC TCAGCCCAGC 
CCACAGACTT CTGAGCGGCC CACCATCCTT TCTCCTCTGG AAATAAGCCC TGAAACACAG 
GCAGCTTTGA TCAGAGGGGA GGATTCCACA GTAGCGGCCC CCAAACAGCA AGTACCGACG 
AGGATGCTTG ATTCTAATAA GCAGGCAACA CTAAGCACCA CAGAGTTAAA TACTGAGCTT 
GCAACACCAT CATTTCCCCT TCTGGAAACT TCTAATGAAA CCAGTTTCCT GATTGGCATT 
AATGAAGAGT CAGTGGAAGG CACAGCAGTC TATTTACCAG GACCTGATCG TTGCAAAATG 
AACCCGTGCC TCAATGGGGG CACCTGTTAT CCTACTGAAA CTTCCTACGT GTGCACCTGC 
GTGCCAGGAT ACAGTGGTGA TCGGTGTGAA CTTGATTTTG ATGAATGTCA TTCTAATCCT 
TGTCGCAATG GAGCCACATG TATTGACGGT TTTAATACCT TCAGGTGTCT CTGCCTCCCG 
AGCTACGTTG GTGCGCTTTG TGAGCAAGAC ACGGAGACAT GTGACTATGG CTGGCACAAA 
TTTCAAGGGC AGTGCTACAA GTACTTTGCC CATCGACGGA CGTGGGATGC AGCTGAACGG 
GAATGCCGTC TGCAGGGCGC CCATCTCACC AGTATCCTGT CTCACGAGGA ACAAATGTTT 
GTGAATCGTG TGGGCCATGA TTATCAGTGG ATAGGTCTCA ATGACAAGAT GTTTGAGCAT 
GACTTCCGTT GGACTGATGG CAGCACACTG CAATATGAGA ACTGGAGGCC GAACCAACCA 
GACAGCTTCT TTTCTACTGG AGAAGATTGT GTTGTGATTA TATGGCATGA GAATGGCCAG 
TGGAATGACG TTCCCTGCAA TTACCATCTC ACCTACACCT GCAAGAAAGG AACAGTTGCT 
TGCGGCCAGC CCCCTGTTGT AGAAAATGCC AAGACCTTTG GAAAGATGAA ACCTCGTTAT 
GAAATCAACT CCTTGATTAG ATATCACTGC AAAGATGGTT TCATTCAACG CCACCTTCCA 
ACTATCCGTT GCCTAGGAAA TGGAAGATGG GCTATGCCTA AAATTACCTG CCTGAACCCA 
TCTGCATACC AAAGGACTTA TTCTAAGAAA TATTTTAAAA ATTCCTCATC AGCAAAGGAC 
AATTCAATAA ATACATCAAA ACATGATCAC CGTTGGAGCC GAAGGTGGCA AGAATCAAGG 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment.
Spine36(14)E921-8(2011 Jun)
Desmoulin GT,Hewitt CR,Hunter CJ

Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi.
Spine35(15)1437-44(2010 Jul)
Desmoulin GT,Reno CR,Hunter CJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Versican V2 is a major extracellular matrix component of the mature bovine brain.
The Journal of biological chemistry273(25)15758-64(1998 Jun)
Schmalfeldt M,Dours-Zimmermann MT,Winterhalter KH,Zimmermann DR

Interaction of a brain extracellular matrix protein with hyaluronic acid.
Biochimica et biophysica acta1075(3)248-58(1991 Oct)
Perides G,Biviano F,Bignami A

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VCAN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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