CACNA1A cDNA ORF clone, Bos taurus(cattle)

The following CACNA1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235770 XM_024993848.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235771 XM_024993849.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235772 XM_024993850.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235773 XM_024993851.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235774 XM_024993852.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235775 XM_024993853.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235776 XM_024993854.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235777 XM_024993855.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235778 XM_024993856.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235779 XM_024993858.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa235780 XM_024993859.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa01216 NM_001075129.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235770
    Clone ID Related Accession (Same CDS sequence) XM_024993848.1
    Accession Version XM_024993848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA
    TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC
    CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG
    CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC
    ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA
    AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC
    CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC
    GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC
    ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT
    ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG
    CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG
    CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC
    GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG
    CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT
    CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC
    CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC
    GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG
    CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC
    AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC
    GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849616.1
    CDS230..7735
    Translation

    Target ORF information:

    RefSeq Version XM_024993848.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA
    TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC
    CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG
    CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC
    ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA
    AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC
    CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC
    GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC
    ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT
    ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG
    CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG
    CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC
    GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG
    CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT
    CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC
    CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC
    GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG
    CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC
    AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC
    GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235771
    Clone ID Related Accession (Same CDS sequence) XM_024993849.1
    Accession Version XM_024993849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC
    CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG
    CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC
    ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA
    AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC
    CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC
    GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC
    ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT
    ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG
    CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG
    CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC
    GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG
    CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT
    CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC
    CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC
    GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG
    CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC
    AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC
    GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849617.1
    CDS230..7735
    Translation

    Target ORF information:

    RefSeq Version XM_024993849.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC
    CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG
    CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC
    ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA
    AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC
    CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC
    GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC
    ATGGCCCACC GTCAGGGCAG TAGTTCCGTA AGTGGAAGCC CAGCCCCCTC AACATCTGGT
    ACCAGCACTC CGCGGCGGGG ACGCCGCCAG CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG
    CACGTGTCCT ATTCCCCCGT GATCCGTAAG GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG
    CAGCCCGCGG CGCGGCCGGG CCGAACGGCC CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC
    GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT CCCACGGGAG GCCACGGAAG CAGCCGCTCG
    CCCGCGATGG AGCGGCGGAT CCCGGGCCCG GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT
    CGCCACGGCG GGGCGCGATG GCCGGCGTCC GGCGCGCACG CGGCCGAGGG CCCCCCGGGC
    CCCCGGCACC ACCACGGTTA CTACCGGGGC TCCGACTACG ACGAGCCAGA CGGCCCGGGC
    GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG GCCGCCGCCT ACGACGCGCC GCCCCCCGCG
    CGCCACGGGT GCTCGCCCAG GACTCCCCGG GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC
    AGGCACGGCC GGCGACTCCC GAACGGCTAC TACCCGGCCC ACGGACTGGC CAGGCCCCGC
    GGGCCGGGCT CCCGGAAAGG CCTGCACGAA GCCTACAGCG AGACCGACGA CGATGACTGG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235772
    Clone ID Related Accession (Same CDS sequence) XM_024993850.1
    Accession Version XM_024993850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG
    CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT
    CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG
    CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG
    AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG
    AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT
    TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG
    GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG
    AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG
    GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG
    AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG
    AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG
    ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC
    GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC
    CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG
    AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG
    GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC
    GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG
    CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC
    GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG
    GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG
    TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC
    CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC
    AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC
    AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC
    GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC
    CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT
    GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC
    CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA
    GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC
    TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC
    CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG
    CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACGAGCAGTA CACGTGGCAA TAGCAAAGGA
    AAGGACATCA ACACGATTAA ATCCCTCCGA GTCCTCCGGG TGCTACGACC TCTTAAAACC
    ATCAAGCGGT TGCCAAAGCT CAAGGCCGTG TTTGACTGCG TGGTGAACTC TCTCAAGAAT
    GTCTTCAACA TCCTCATCGT CTACATGCTG TTCATGTTCA TCTTCGCCGT GGTGGCCGTG
    CAGCTCTTCA AGGGCAAGTT TTTCCACTGC ACAGATGAGT CCAAGGAGTT TGAGAAAGAT
    TGCCGCGGCA AGTACCTCCT CTATGAGAAG AATGAGGTAA AGGCGAGGGA TCGAGAGTGG
    AAGAAGTATG AGTTCCATTA TGACAATGTG CTGTGGGCGC TGCTGACCCT CTTCACCGTG
    TCCACGGGAG AAGGCTGGCC ACAGGTCCTC AAGCACTCCG TGGACGCCAC CTTCGAGAAC
    CAGGGCCCCA GCCCCGGGTA CCGCATGGAG ATGTCCATCT TCTACGTCGT CTACTTTGTG
    GTGTTCCCCT TCTTCTTTGT CAACATCTTT GTGGCCTTGA TCATCATCAC CTTCCAGGAG
    CAGGGGGACA AGATGATGGA GGAGTACAGC CTGGAGAAAA ATGAGAGGGC GTGCATCGAC
    TTCGCCATCA GCGCCAAACC CCTGACCCGG CACATGCCGC AGAACAAGCA GAGTTTCCAG
    TACCGCATGT GGCAGTTCGT GGTGTCCCCG CCTTTCGAGT ACACCATCAT GGCCATGATC
    GCCCTCAACA CCATCGTGCT CATGATGAAG TTCTACGGGG CCTCTGTTGC TTATGAGAAT
    GCTCTGAGAG TATTCAACAT CGTCTTCACC TCCCTCTTCT CACTGGAATG TGTGCTGAAA
    GTCATGGCTT TTGGGATTCT GAATTATTTT CGCGATGCCT GGAACATCTT CGACTTTGTG
    ACTGTTCTGG GCAGCATCAC CGACATCCTC GTGACCGAGT TTGGGAATAA CTTCATCAAC
    TTGAGCTTCC TCCGCCTCTT CCGAGCTGCC CGGCTCATCA AACTCCTCCG TCAGGGTTAC
    ACCATCCGAA TTCTTCTCTG GACCTTTGTG CAGTCCTTCA AGGCCCTGCC CTATGTCTGT
    CTACTGATTG CCATGCTTTT TTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGCAAC
    ATTGGCATCG ATGTGGAGGA CGAGGACAGT GATGAAGATG AATTCCAAAT CACTGAGCAC
    AATAACTTCC GCACCTTCTT CCAGGCCCTC ATGCTTCTCT TCCGGAGTGC CACTGGGGAG
    GCTTGGCACA ACATCATGCT TTCCTGCCTC AGCGGGAAAC CCTGTGATAA GAACTCTGGC
    ATCCTGACTC ACGAATGCGG CAATGAATTT GCTTATTTTT ACTTCGTTTC CTTCATCTTC
    CTCTGCTCGT TTCTGATGCT GAATCTTTTT GTTGCCGTCA TCATGGACAA CTTTGAGTAC
    CTCACCCGAG ATTCCTCCAT CCTGGGTCCC CACCACCTGG ATGAGTACGT GCGTGTCTGG
    GCCGAGTATG ACCCTGCAGC GTGCGGTCGG ATTCATTATA AGGATATGTA CAGTTTATTG
    CGCGTAATAT CTCCCCCTCT CGGCTTAGGC AAGAAATGTC CTCATAGGGT TGCTTGCAAG
    AGGCTCCTGC GGATGGATCT GCCCGTCGCT GATGACAACA CCGTCCACTT CAACTCTACC
    CTCATGGCTC TGATCCGCAC GGCCCTGGAC ATCAAAATTG CCAAAGGTGG AGCTGACAAA
    CAACAAATGG ATGCTGAGTT GCGGAAGGAG ATGATGGCCA TCTGGCCCAA TCTGTCCCAG
    AAGACGCTGG ACCTGCTGGT CACCCCCCAC AAGTCCACGG ACCTGACAGT GGGGAAGATC
    TATGCGGCCA TGATGATCAT GGAGTATTAC CGGCAGAGCA AGGCCAAGAA GCTTCAGGCC
    ATGCGCGAGG AACAGAACCG GACACCTCTC ATGTTCCAGC GCATGGAGCC CCCATCGCCA
    ACACAGGAGG GGGGGCCTGG CCAGAACGCC CTCCCCTCCA CCCAGCTGGA CCCAGGAGGA
    GGCTTGATGG CTCACGAAAG TGGCATGAAA GAGAGCCCAT CCTGGGTGAC CCAGCGGGCC
    CAGGAGATGT TCCAGAAGAC GGGCACATGG AGCCCAGAGC GGGGCCCCCC CACTGATATG
    CCTAATAGCC AACCCAACTC TCAGTCTGTG GAGATGCGAG AGATGGGCAG AGACGGCTAC
    TCTGACAGTG AGCACTACTT CCCCGTTGAA GGCCAGGCCC GGGCCGCCTC CATGCCCCGC
    CTGCCTGCCG AGAACCAGAG GAGAAGGGGC CGGCCACGTG GGAATAACCT CAGTACCATC
    TCAGACACCA GCCCCATGAA GCGCTCGGCC TCCGTGCTGG GCCCCAAGGC CCGGCGCCTG
    GACGACTACT CCCTGGAGAG GGTGCCGCCC GAGGAGAACC AGCGGCACCA CCAGAGGCGC
    AGAGACCGTG GCCACCGCAC CTCCGAGCGC TCCTTGGGCC GCTACACGGA CGTGGACACA
    GGCTTGGGGA CAGACTTGAG CATGACCACC CAGTCTGGGG ACCTGCCATC AAAGGAAAGA
    GACCAGGAGC GGGGCCGGCC CAAGGACCGG AAGCATCGGC AACACCACCA CCACCACCAC
    CACCACCATC CCCCGCCCCC TGACAAGGAG CGCTACACCC AGGAGCGGCC TGAGCACGGC
    CGGCCCCGGG CCCGTGACCA GCGCTGGTCC CGCTCACCCA GTGAGGGCCG GGAGCACATG
    GCCCACCGTC AGGGCAGTAG TTCCGTAAGT GGAAGCCCAG CCCCCTCAAC ATCTGGTACC
    AGCACTCCGC GGCGGGGACG CCGCCAGCTC CCCCAGACCC CCTCCACTCC CCGGCCGCAC
    GTGTCCTATT CCCCCGTGAT CCGTAAGGCC GGCGGCTCGG GTCCCCAGCA GCAGCAGCAG
    CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG
    GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC
    GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC
    CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC
    CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGCCAGACGG CCCGGGCGGC
    GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC
    CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG
    CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG
    CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA CCGACGACGA TGACTGGTGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849618.1
    CDS230..7732
    Translation

    Target ORF information:

    RefSeq Version XM_024993850.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG
    CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT
    CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG
    CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG
    AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG
    AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT
    TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG
    GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG
    AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG
    GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG
    AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG
    AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG
    ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC
    GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC
    CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG
    AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG
    GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC
    GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG
    CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC
    GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG
    GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG
    TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC
    CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC
    AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC
    AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC
    GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC
    CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT
    GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC
    CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA
    GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC
    TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC
    CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG
    CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACGAGCAGTA CACGTGGCAA TAGCAAAGGA
    AAGGACATCA ACACGATTAA ATCCCTCCGA GTCCTCCGGG TGCTACGACC TCTTAAAACC
    ATCAAGCGGT TGCCAAAGCT CAAGGCCGTG TTTGACTGCG TGGTGAACTC TCTCAAGAAT
    GTCTTCAACA TCCTCATCGT CTACATGCTG TTCATGTTCA TCTTCGCCGT GGTGGCCGTG
    CAGCTCTTCA AGGGCAAGTT TTTCCACTGC ACAGATGAGT CCAAGGAGTT TGAGAAAGAT
    TGCCGCGGCA AGTACCTCCT CTATGAGAAG AATGAGGTAA AGGCGAGGGA TCGAGAGTGG
    AAGAAGTATG AGTTCCATTA TGACAATGTG CTGTGGGCGC TGCTGACCCT CTTCACCGTG
    TCCACGGGAG AAGGCTGGCC ACAGGTCCTC AAGCACTCCG TGGACGCCAC CTTCGAGAAC
    CAGGGCCCCA GCCCCGGGTA CCGCATGGAG ATGTCCATCT TCTACGTCGT CTACTTTGTG
    GTGTTCCCCT TCTTCTTTGT CAACATCTTT GTGGCCTTGA TCATCATCAC CTTCCAGGAG
    CAGGGGGACA AGATGATGGA GGAGTACAGC CTGGAGAAAA ATGAGAGGGC GTGCATCGAC
    TTCGCCATCA GCGCCAAACC CCTGACCCGG CACATGCCGC AGAACAAGCA GAGTTTCCAG
    TACCGCATGT GGCAGTTCGT GGTGTCCCCG CCTTTCGAGT ACACCATCAT GGCCATGATC
    GCCCTCAACA CCATCGTGCT CATGATGAAG TTCTACGGGG CCTCTGTTGC TTATGAGAAT
    GCTCTGAGAG TATTCAACAT CGTCTTCACC TCCCTCTTCT CACTGGAATG TGTGCTGAAA
    GTCATGGCTT TTGGGATTCT GAATTATTTT CGCGATGCCT GGAACATCTT CGACTTTGTG
    ACTGTTCTGG GCAGCATCAC CGACATCCTC GTGACCGAGT TTGGGAATAA CTTCATCAAC
    TTGAGCTTCC TCCGCCTCTT CCGAGCTGCC CGGCTCATCA AACTCCTCCG TCAGGGTTAC
    ACCATCCGAA TTCTTCTCTG GACCTTTGTG CAGTCCTTCA AGGCCCTGCC CTATGTCTGT
    CTACTGATTG CCATGCTTTT TTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGCAAC
    ATTGGCATCG ATGTGGAGGA CGAGGACAGT GATGAAGATG AATTCCAAAT CACTGAGCAC
    AATAACTTCC GCACCTTCTT CCAGGCCCTC ATGCTTCTCT TCCGGAGTGC CACTGGGGAG
    GCTTGGCACA ACATCATGCT TTCCTGCCTC AGCGGGAAAC CCTGTGATAA GAACTCTGGC
    ATCCTGACTC ACGAATGCGG CAATGAATTT GCTTATTTTT ACTTCGTTTC CTTCATCTTC
    CTCTGCTCGT TTCTGATGCT GAATCTTTTT GTTGCCGTCA TCATGGACAA CTTTGAGTAC
    CTCACCCGAG ATTCCTCCAT CCTGGGTCCC CACCACCTGG ATGAGTACGT GCGTGTCTGG
    GCCGAGTATG ACCCTGCAGC GTGCGGTCGG ATTCATTATA AGGATATGTA CAGTTTATTG
    CGCGTAATAT CTCCCCCTCT CGGCTTAGGC AAGAAATGTC CTCATAGGGT TGCTTGCAAG
    AGGCTCCTGC GGATGGATCT GCCCGTCGCT GATGACAACA CCGTCCACTT CAACTCTACC
    CTCATGGCTC TGATCCGCAC GGCCCTGGAC ATCAAAATTG CCAAAGGTGG AGCTGACAAA
    CAACAAATGG ATGCTGAGTT GCGGAAGGAG ATGATGGCCA TCTGGCCCAA TCTGTCCCAG
    AAGACGCTGG ACCTGCTGGT CACCCCCCAC AAGTCCACGG ACCTGACAGT GGGGAAGATC
    TATGCGGCCA TGATGATCAT GGAGTATTAC CGGCAGAGCA AGGCCAAGAA GCTTCAGGCC
    ATGCGCGAGG AACAGAACCG GACACCTCTC ATGTTCCAGC GCATGGAGCC CCCATCGCCA
    ACACAGGAGG GGGGGCCTGG CCAGAACGCC CTCCCCTCCA CCCAGCTGGA CCCAGGAGGA
    GGCTTGATGG CTCACGAAAG TGGCATGAAA GAGAGCCCAT CCTGGGTGAC CCAGCGGGCC
    CAGGAGATGT TCCAGAAGAC GGGCACATGG AGCCCAGAGC GGGGCCCCCC CACTGATATG
    CCTAATAGCC AACCCAACTC TCAGTCTGTG GAGATGCGAG AGATGGGCAG AGACGGCTAC
    TCTGACAGTG AGCACTACTT CCCCGTTGAA GGCCAGGCCC GGGCCGCCTC CATGCCCCGC
    CTGCCTGCCG AGAACCAGAG GAGAAGGGGC CGGCCACGTG GGAATAACCT CAGTACCATC
    TCAGACACCA GCCCCATGAA GCGCTCGGCC TCCGTGCTGG GCCCCAAGGC CCGGCGCCTG
    GACGACTACT CCCTGGAGAG GGTGCCGCCC GAGGAGAACC AGCGGCACCA CCAGAGGCGC
    AGAGACCGTG GCCACCGCAC CTCCGAGCGC TCCTTGGGCC GCTACACGGA CGTGGACACA
    GGCTTGGGGA CAGACTTGAG CATGACCACC CAGTCTGGGG ACCTGCCATC AAAGGAAAGA
    GACCAGGAGC GGGGCCGGCC CAAGGACCGG AAGCATCGGC AACACCACCA CCACCACCAC
    CACCACCATC CCCCGCCCCC TGACAAGGAG CGCTACACCC AGGAGCGGCC TGAGCACGGC
    CGGCCCCGGG CCCGTGACCA GCGCTGGTCC CGCTCACCCA GTGAGGGCCG GGAGCACATG
    GCCCACCGTC AGGGCAGTAG TTCCGTAAGT GGAAGCCCAG CCCCCTCAAC ATCTGGTACC
    AGCACTCCGC GGCGGGGACG CCGCCAGCTC CCCCAGACCC CCTCCACTCC CCGGCCGCAC
    GTGTCCTATT CCCCCGTGAT CCGTAAGGCC GGCGGCTCGG GTCCCCAGCA GCAGCAGCAG
    CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG
    GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC
    GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC
    CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC
    CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGCCAGACGG CCCGGGCGGC
    GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC
    CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG
    CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG
    CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA CCGACGACGA TGACTGGTGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235773
    Clone ID Related Accession (Same CDS sequence) XM_024993851.1
    Accession Version XM_024993851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC
    AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG
    TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC
    ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC
    AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC
    AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT
    GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA
    GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC
    AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC
    TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC
    AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC
    AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG
    TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC
    ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA
    GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT
    TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG
    GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC
    CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA
    ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT
    GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC
    GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC
    CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC
    AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT
    CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG
    TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA
    GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT
    GACCCTGCAG CGTGCGGTCG GATTCATTAT AAGGATATGT ACAGTTTATT GCGCGTAATA
    TCTCCCCCTC TCGGCTTAGG CAAGAAATGT CCTCATAGGG TTGCTTGCAA GAGGCTCCTG
    CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT
    CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG
    GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG
    GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC
    ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG
    GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG
    GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG
    GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG
    TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC
    CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT
    GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC
    GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC
    AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC
    TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT
    GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG
    ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG
    CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT
    CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG
    GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT
    CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG
    CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT
    TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG
    CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC
    CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG
    CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG
    GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC
    CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG
    GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC
    TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG
    CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC
    CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849619.1
    CDS230..7723
    Translation

    Target ORF information:

    RefSeq Version XM_024993851.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC
    AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG
    TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC
    ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC
    AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC
    AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT
    GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA
    GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC
    AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC
    TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC
    AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC
    AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG
    TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC
    ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA
    GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT
    TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG
    GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC
    CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA
    ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT
    GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC
    GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC
    CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC
    AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT
    CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG
    TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA
    GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT
    GACCCTGCAG CGTGCGGTCG GATTCATTAT AAGGATATGT ACAGTTTATT GCGCGTAATA
    TCTCCCCCTC TCGGCTTAGG CAAGAAATGT CCTCATAGGG TTGCTTGCAA GAGGCTCCTG
    CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT
    CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG
    GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG
    GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC
    ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG
    GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG
    GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG
    GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG
    TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC
    CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT
    GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC
    GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC
    AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC
    TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT
    GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG
    ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG
    CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT
    CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG
    GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT
    CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG
    CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT
    TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG
    CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC
    CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG
    CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG
    GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC
    CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG
    GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC
    TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG
    CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC
    CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235774
    Clone ID Related Accession (Same CDS sequence) XM_024993852.1
    Accession Version XM_024993852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC
    AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG
    TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC
    ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC
    AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC
    AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT
    GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA
    GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC
    AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC
    TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC
    AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC
    AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG
    TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC
    ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA
    GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT
    TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG
    GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC
    CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA
    ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT
    GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC
    GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC
    CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC
    AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT
    CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG
    TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA
    GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT
    GACCCTGCAG CGTGGGGCCG CATGCCTTAC CCGGACATGT ATCAGATGCT GAGACACATG
    TCTCCGCCCC TGGGTCTGGG GAAGAAGTGT CCGGCCAGAG TGGCTTACAA GAGGCTCCTG
    CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT
    CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG
    GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG
    GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC
    ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG
    GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG
    GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG
    GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG
    TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC
    CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT
    GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC
    GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC
    AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC
    TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT
    GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG
    ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG
    CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT
    CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG
    GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT
    CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG
    CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT
    TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG
    CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC
    CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG
    CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG
    GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC
    CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG
    GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC
    TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG
    CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC
    CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849620.1
    CDS230..7723
    Translation

    Target ORF information:

    RefSeq Version XM_024993852.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACTGGCA ATAGCAAAGG AAAGGACATC
    AACACGATTA AATCCCTCCG AGTCCTCCGG GTGCTACGAC CTCTTAAAAC CATCAAGCGG
    TTGCCAAAGC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTCAAGAA TGTCTTCAAC
    ATCCTCATCG TCTACATGCT GTTCATGTTC ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC
    AAGGGCAAGT TTTTCCACTG CACAGATGAG TCCAAGGAGT TTGAGAAAGA TTGCCGCGGC
    AAGTACCTCC TCTATGAGAA GAATGAGGTA AAGGCGAGGG ATCGAGAGTG GAAGAAGTAT
    GAGTTCCATT ATGACAATGT GCTGTGGGCG CTGCTGACCC TCTTCACCGT GTCCACGGGA
    GAAGGCTGGC CACAGGTCCT CAAGCACTCC GTGGACGCCA CCTTCGAGAA CCAGGGCCCC
    AGCCCCGGGT ACCGCATGGA GATGTCCATC TTCTACGTCG TCTACTTTGT GGTGTTCCCC
    TTCTTCTTTG TCAACATCTT TGTGGCCTTG ATCATCATCA CCTTCCAGGA GCAGGGGGAC
    AAGATGATGG AGGAGTACAG CCTGGAGAAA AATGAGAGGG CGTGCATCGA CTTCGCCATC
    AGCGCCAAAC CCCTGACCCG GCACATGCCG CAGAACAAGC AGAGTTTCCA GTACCGCATG
    TGGCAGTTCG TGGTGTCCCC GCCTTTCGAG TACACCATCA TGGCCATGAT CGCCCTCAAC
    ACCATCGTGC TCATGATGAA GTTCTACGGG GCCTCTGTTG CTTATGAGAA TGCTCTGAGA
    GTATTCAACA TCGTCTTCAC CTCCCTCTTC TCACTGGAAT GTGTGCTGAA AGTCATGGCT
    TTTGGGATTC TGAATTATTT TCGCGATGCC TGGAACATCT TCGACTTTGT GACTGTTCTG
    GGCAGCATCA CCGACATCCT CGTGACCGAG TTTGGGAATA ACTTCATCAA CTTGAGCTTC
    CTCCGCCTCT TCCGAGCTGC CCGGCTCATC AAACTCCTCC GTCAGGGTTA CACCATCCGA
    ATTCTTCTCT GGACCTTTGT GCAGTCCTTC AAGGCCCTGC CCTATGTCTG TCTACTGATT
    GCCATGCTTT TTTTCATCTA CGCCATCATT GGGATGCAGG TGTTTGGCAA CATTGGCATC
    GATGTGGAGG ACGAGGACAG TGATGAAGAT GAATTCCAAA TCACTGAGCA CAATAACTTC
    CGCACCTTCT TCCAGGCCCT CATGCTTCTC TTCCGGAGTG CCACTGGGGA GGCTTGGCAC
    AACATCATGC TTTCCTGCCT CAGCGGGAAA CCCTGTGATA AGAACTCTGG CATCCTGACT
    CACGAATGCG GCAATGAATT TGCTTATTTT TACTTCGTTT CCTTCATCTT CCTCTGCTCG
    TTTCTGATGC TGAATCTTTT TGTTGCCGTC ATCATGGACA ACTTTGAGTA CCTCACCCGA
    GATTCCTCCA TCCTGGGTCC CCACCACCTG GATGAGTACG TGCGTGTCTG GGCCGAGTAT
    GACCCTGCAG CGTGGGGCCG CATGCCTTAC CCGGACATGT ATCAGATGCT GAGACACATG
    TCTCCGCCCC TGGGTCTGGG GAAGAAGTGT CCGGCCAGAG TGGCTTACAA GAGGCTCCTG
    CGGATGGATC TGCCCGTCGC TGATGACAAC ACCGTCCACT TCAACTCTAC CCTCATGGCT
    CTGATCCGCA CGGCCCTGGA CATCAAAATT GCCAAAGGTG GAGCTGACAA ACAACAAATG
    GATGCTGAGT TGCGGAAGGA GATGATGGCC ATCTGGCCCA ATCTGTCCCA GAAGACGCTG
    GACCTGCTGG TCACCCCCCA CAAGTCCACG GACCTGACAG TGGGGAAGAT CTATGCGGCC
    ATGATGATCA TGGAGTATTA CCGGCAGAGC AAGGCCAAGA AGCTTCAGGC CATGCGCGAG
    GAACAGAACC GGACACCTCT CATGTTCCAG CGCATGGAGC CCCCATCGCC AACACAGGAG
    GGGGGGCCTG GCCAGAACGC CCTCCCCTCC ACCCAGCTGG ACCCAGGAGG AGGCTTGATG
    GCTCACGAAA GTGGCATGAA AGAGAGCCCA TCCTGGGTGA CCCAGCGGGC CCAGGAGATG
    TTCCAGAAGA CGGGCACATG GAGCCCAGAG CGGGGCCCCC CCACTGATAT GCCTAATAGC
    CAACCCAACT CTCAGTCTGT GGAGATGCGA GAGATGGGCA GAGACGGCTA CTCTGACAGT
    GAGCACTACT TCCCCGTTGA AGGCCAGGCC CGGGCCGCCT CCATGCCCCG CCTGCCTGCC
    GAGAACCAGA GGAGAAGGGG CCGGCCACGT GGGAATAACC TCAGTACCAT CTCAGACACC
    AGCCCCATGA AGCGCTCGGC CTCCGTGCTG GGCCCCAAGG CCCGGCGCCT GGACGACTAC
    TCCCTGGAGA GGGTGCCGCC CGAGGAGAAC CAGCGGCACC ACCAGAGGCG CAGAGACCGT
    GGCCACCGCA CCTCCGAGCG CTCCTTGGGC CGCTACACGG ACGTGGACAC AGGCTTGGGG
    ACAGACTTGA GCATGACCAC CCAGTCTGGG GACCTGCCAT CAAAGGAAAG AGACCAGGAG
    CGGGGCCGGC CCAAGGACCG GAAGCATCGG CAACACCACC ACCACCACCA CCACCACCAT
    CCCCCGCCCC CTGACAAGGA GCGCTACACC CAGGAGCGGC CTGAGCACGG CCGGCCCCGG
    GCCCGTGACC AGCGCTGGTC CCGCTCACCC AGTGAGGGCC GGGAGCACAT GGCCCACCGT
    CAGGGCAGTA GTTCCGTAAG TGGAAGCCCA GCCCCCTCAA CATCTGGTAC CAGCACTCCG
    CGGCGGGGAC GCCGCCAGCT CCCCCAGACC CCCTCCACTC CCCGGCCGCA CGTGTCCTAT
    TCCCCCGTGA TCCGTAAGGC CGGCGGCTCG GGTCCCCAGC AGCAGCAGCA GCCCGCGGCG
    CGGCCGGGCC GAACGGCCCC CAGCGGCCCG CGGAGGTACC CGGGCCCCGT GGCCGAGCCC
    CTGGCCGGGG AGCGGCCTCC CACGGGAGGC CACGGAAGCA GCCGCTCGCC CGCGATGGAG
    CGGCGGATCC CGGGCCCGGC CCGGAGCGAG TCCCCCAGGG CGCCGTGTCG CCACGGCGGG
    GCGCGATGGC CGGCGTCCGG CGCGCACGCG GCCGAGGGCC CCCCGGGCCC CCGGCACCAC
    CACGGTTACT ACCGGGGCTC CGACTACGAC GAGCCAGACG GCCCGGGCGG CGGCGGCGGG
    GAGGAGGAGG AGCCCCGGGC CGCCGCCTAC GACGCGCCGC CCCCCGCGCG CCACGGGTGC
    TCGCCCAGGA CTCCCCGGGC CGCGGGCCCG GCCTGCGCCT CGCCTTCCAG GCACGGCCGG
    CGACTCCCGA ACGGCTACTA CCCGGCCCAC GGACTGGCCA GGCCCCGCGG GCCGGGCTCC
    CGGAAAGGCC TGCACGAAGC CTACAGCGAG ACCGACGACG ATGACTGGTG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235775
    Clone ID Related Accession (Same CDS sequence) XM_024993853.1
    Accession Version XM_024993853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG
    CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT
    CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG
    CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG
    AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG
    AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT
    TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG
    GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG
    AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG
    GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG
    AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG
    AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG
    ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC
    GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC
    CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG
    AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG
    GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC
    GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG
    CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC
    GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG
    GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG
    TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC
    CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC
    AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC
    AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC
    GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC
    CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT
    GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC
    CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA
    GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC
    TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC
    CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG
    CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA GGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGTTG
    CCAAAGCTCA AGGCCGTGTT TGACTGCGTG GTGAACTCTC TCAAGAATGT CTTCAACATC
    CTCATCGTCT ACATGCTGTT CATGTTCATC TTCGCCGTGG TGGCCGTGCA GCTCTTCAAG
    GGCAAGTTTT TCCACTGCAC AGATGAGTCC AAGGAGTTTG AGAAAGATTG CCGCGGCAAG
    TACCTCCTCT ATGAGAAGAA TGAGGTAAAG GCGAGGGATC GAGAGTGGAA GAAGTATGAG
    TTCCATTATG ACAATGTGCT GTGGGCGCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCACTCCGTG GACGCCACCT TCGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATCTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA GGGGGACAAG
    ATGATGGAGG AGTACAGCCT GGAGAAAAAT GAGAGGGCGT GCATCGACTT CGCCATCAGC
    GCCAAACCCC TGACCCGGCA CATGCCGCAG AACAAGCAGA GTTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCCCCGCC TTTCGAGTAC ACCATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTCA TGATGAAGTT CTACGGGGCC TCTGTTGCTT ATGAGAATGC TCTGAGAGTA
    TTCAACATCG TCTTCACCTC CCTCTTCTCA CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTTCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ACATCCTCGT GACCGAGTTT GGGAATAACT TCATCAACTT GAGCTTCCTC
    CGCCTCTTCC GAGCTGCCCG GCTCATCAAA CTCCTCCGTC AGGGTTACAC CATCCGAATT
    CTTCTCTGGA CCTTTGTGCA GTCCTTCAAG GCCCTGCCCT ATGTCTGTCT ACTGATTGCC
    ATGCTTTTTT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGCAACAT TGGCATCGAT
    GTGGAGGACG AGGACAGTGA TGAAGATGAA TTCCAAATCA CTGAGCACAA TAACTTCCGC
    ACCTTCTTCC AGGCCCTCAT GCTTCTCTTC CGGAGTGCCA CTGGGGAGGC TTGGCACAAC
    ATCATGCTTT CCTGCCTCAG CGGGAAACCC TGTGATAAGA ACTCTGGCAT CCTGACTCAC
    GAATGCGGCA ATGAATTTGC TTATTTTTAC TTCGTTTCCT TCATCTTCCT CTGCTCGTTT
    CTGATGCTGA ATCTTTTTGT TGCCGTCATC ATGGACAACT TTGAGTACCT CACCCGAGAT
    TCCTCCATCC TGGGTCCCCA CCACCTGGAT GAGTACGTGC GTGTCTGGGC CGAGTATGAC
    CCTGCAGCGT GGGGCCGCAT GCCTTACCCG GACATGTATC AGATGCTGAG ACACATGTCT
    CCGCCCCTGG GTCTGGGGAA GAAGTGTCCG GCCAGAGTGG CTTACAAGAG GCTCCTGCGG
    ATGGATCTGC CCGTCGCTGA TGACAACACC GTCCACTTCA ACTCTACCCT CATGGCTCTG
    ATCCGCACGG CCCTGGACAT CAAAATTGCC AAAGGTGGAG CTGACAAACA ACAAATGGAT
    GCTGAGTTGC GGAAGGAGAT GATGGCCATC TGGCCCAATC TGTCCCAGAA GACGCTGGAC
    CTGCTGGTCA CCCCCCACAA GTCCACGGAC CTGACAGTGG GGAAGATCTA TGCGGCCATG
    ATGATCATGG AGTATTACCG GCAGAGCAAG GCCAAGAAGC TTCAGGCCAT GCGCGAGGAA
    CAGAACCGGA CACCTCTCAT GTTCCAGCGC ATGGAGCCCC CATCGCCAAC ACAGGAGGGG
    GGGCCTGGCC AGAACGCCCT CCCCTCCACC CAGCTGGACC CAGGAGGAGG CTTGATGGCT
    CACGAAAGTG GCATGAAAGA GAGCCCATCC TGGGTGACCC AGCGGGCCCA GGAGATGTTC
    CAGAAGACGG GCACATGGAG CCCAGAGCGG GGCCCCCCCA CTGATATGCC TAATAGCCAA
    CCCAACTCTC AGTCTGTGGA GATGCGAGAG ATGGGCAGAG ACGGCTACTC TGACAGTGAG
    CACTACTTCC CCGTTGAAGG CCAGGCCCGG GCCGCCTCCA TGCCCCGCCT GCCTGCCGAG
    AACCAGAGGA GAAGGGGCCG GCCACGTGGG AATAACCTCA GTACCATCTC AGACACCAGC
    CCCATGAAGC GCTCGGCCTC CGTGCTGGGC CCCAAGGCCC GGCGCCTGGA CGACTACTCC
    CTGGAGAGGG TGCCGCCCGA GGAGAACCAG CGGCACCACC AGAGGCGCAG AGACCGTGGC
    CACCGCACCT CCGAGCGCTC CTTGGGCCGC TACACGGACG TGGACACAGG CTTGGGGACA
    GACTTGAGCA TGACCACCCA GTCTGGGGAC CTGCCATCAA AGGAAAGAGA CCAGGAGCGG
    GGCCGGCCCA AGGACCGGAA GCATCGGCAA CACCACCACC ACCACCACCA CCACCATCCC
    CCGCCCCCTG ACAAGGAGCG CTACACCCAG GAGCGGCCTG AGCACGGCCG GCCCCGGGCC
    CGTGACCAGC GCTGGTCCCG CTCACCCAGT GAGGGCCGGG AGCACATGGC CCACCGTCAG
    GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG
    CGGGGACGCC GCCAGCTCCC CCAGACCCCC TCCACTCCCC GGCCGCACGT GTCCTATTCC
    CCCGTGATCC GTAAGGCCGG CGGCTCGGGT CCCCAGCAGC AGCAGCAGCC CGCGGCGCGG
    CCGGGCCGAA CGGCCCCCAG CGGCCCGCGG AGGTACCCGG GCCCCGTGGC CGAGCCCCTG
    GCCGGGGAGC GGCCTCCCAC GGGAGGCCAC GGAAGCAGCC GCTCGCCCGC GATGGAGCGG
    CGGATCCCGG GCCCGGCCCG GAGCGAGTCC CCCAGGGCGC CGTGTCGCCA CGGCGGGGCG
    CGATGGCCGG CGTCCGGCGC GCACGCGGCC GAGGGCCCCC CGGGCCCCCG GCACCACCAC
    GGTTACTACC GGGGCTCCGA CTACGACGAG CCAGACGGCC CGGGCGGCGG CGGCGGGGAG
    GAGGAGGAGC CCCGGGCCGC CGCCTACGAC GCGCCGCCCC CCGCGCGCCA CGGGTGCTCG
    CCCAGGACTC CCCGGGCCGC GGGCCCGGCC TGCGCCTCGC CTTCCAGGCA CGGCCGGCGA
    CTCCCGAACG GCTACTACCC GGCCCACGGA CTGGCCAGGC CCCGCGGGCC GGGCTCCCGG
    AAAGGCCTGC ACGAAGCCTA CAGCGAGACC GACGACGATG ACTGGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849621.1
    CDS230..7720
    Translation

    Target ORF information:

    RefSeq Version XM_024993853.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGCTCTG
    CGGAGAGCCA CTATAAAGAA GAGCAAGACA GACCTGCTCA ACCCCGAGGA GGCTGAGGAT
    CAGCTGGCCG ACATCGCCTC TGTGGGGTCT CCCTTCGCCC GAGCCAGCAT TAAAAGTGCC
    AAGCTGGAGA ACTCAACTTT TTTTCACAAA AAGGAGAGGA GGATGCGTTT CTACATCCGC
    CGCATGGTCA AAACTCAGGC CTTCTACTGG ACTGTGCTCA GTCTGGTAGC CCTCAACACG
    CTGTGTGTCG CTATCGTTCA CTACAACCAG CCTGAGTGGC TCTCCGACTT CCTCTACTAT
    GCAGAATTCA TTTTCTTAGG ACTCTTTATG TCCGAAATGT TTATAAAAAT GTACGGGCTT
    GGGACGCGGC CTTACTTCCA CTCTTCCTTC AACTGTTTTG ATTGTGGGGT CATCATCGGG
    AGTATCTTTG AGGTCATCTG GGCTGTCATA AAACCCGGCA CATCCTTTGG AATCAGTGTG
    TTACGAGCCC TCAGGTTATT GCGTATTTTC AAAGTCACAA AGTACTGGGC ATCTCTCAGG
    AACCTGGTCG TCTCTCTTCT CAACTCCATG AAATCCATCA TCAGCCTGTT GTTCCTCCTT
    TTCCTATTCA TCGTCGTCTT TGCCCTTCTG GGAATGCAAC TCTTTGGCGG CCAGTTTAAT
    TTCGATGAAG GGACTCCTCC TACCAACTTC GACACTTTTC CAGCAGCAAT AATGACAGTT
    TTTCAGATCC TGACGGGCGA AGACTGGAAC GAGGTCATGT ATGACGGCAT CAAGTCTCAG
    GGGGGCGTGC AAGGCGGCAT GGTGTTCTCC ATCTACTTCA TCGTGCTGAC GCTCTTCGGG
    AACTACACCC TCCTGAACGT GTTCTTGGCC ATCGCGGTGG ATAATCTGGC TAACGCCCAG
    GAGCTCACCA AGGATGAACA AGAAGAAGAA GAGGCTGCCA ACCAGAAACT CGCCCTCCAG
    AAAGCCAAAG AGGTGGCAGA AGTGAGTCCT CTGTCTGCAG CCAACATGTC CATAGCTGTG
    AAGGAGCAAC AGAAGAACCA GAAGCCCGCC AAGTCGGTGT GGGAGCAGCG GACAAGCGAG
    ATGAGGAAGC AGAATCTTCT GGCCAGTCGG GAGGCACTCT ACAACGAGAT GGACCCAGAC
    GAGCGCTGGA AGGCCTCCTA CGCTCGCCAC CTGCGGCCCG ACATGAAGAC CCACCTGGAC
    CGGCCGCTGG TGGTCGACCC CCAGGAGAAC CGTAACAACA ACACCAACAA GAGCCGGGCG
    GCCGAGCCCA CGGTGGACCA GCGCCTCGGG CAGCAGCGAG CCGAGGACTT CCTCAGGAAA
    CAGGCCCGCT ACCACGACCG GGCCCGGGAC CCCAGCAGCT CCGTGGGCCT GGACACCCGG
    AGGCCCTGGG CGGGCAGCCA GGAAGCCGAG CTGAGCCGGG AGGGGCCTTA CGGTCGCGAG
    TCGGACCACC ACGCCCGGGA GGGCGGCCTG GAGCAGCCGG GGTTCTGGGA GGGCGAGGCG
    GAGCGGGGCA AGGCGGGCGA CCCGCACCGG AGGCACGCGC ACCGGCAGGC GGGCAGCCGC
    GAGAGCCGCA GCGGGTCCCC GCGCACCGGG GCCGACGGAG AGCCCCGCCG GCACCGGGCG
    CACCGCAGGC CGGGCGACGA GGCCCCGGAC GACAAGGCCG ATCGGAGGTC CCGGCACCGC
    GAGGGCAGCC GGCCGGCCCG GGGCGGCGAG GGCGAGAGCG AGGGCCCAGA CGGCGGCGGC
    GAGCGGAGGC GGCGGCACCG GCATGGCCCT CCCCCAGCGT ACGACGCGGA CATGCGCAGG
    GAGGACAAGG AGCGCAGGCA CCGCAGGAGG AAAGAGAACC AGGGCTCCGG GGTCCCCGTG
    TCAGGCCCCA ACCTGTCGAC CACACGGCCA ATCCAGCAGG ACCTGGGCCG CCAAGACCCC
    CCGCTGGCCG AGGATATCGA CAACATGAAG AACAACAAAC TGGCCACTGC GGAGTCAGCC
    AGTCCCCACG ACAGCCTCAG CCACGCCGGC CTGCCCCAGA GCCCAGCCAA GATGGGGAAC
    AGCATGGACC CTGGCCCCAC ACCGGCCCCC ACCACCACGG CCACCAACCC TCAGAATGCC
    GTCAGTCGCC GGACAGCCAA CAACCCGGGG AACCCGTCCA ACCCCGGCCC CCCCAAGACC
    CCTGAGAACA GCCTTATCGT CACCAACCCC AGCAGCACCC AGGCCAACTC AGCTAAGACT
    GCCAGGAAAC CTGACCACAC CACGGTGGAC ATCCCCCCAG CCTGCCCACC CCCACTCAAC
    CACACCATCA TCCAAGTGAA CAAAAACGCC AACCCAGACC CACTGCCAAA AAAGGAGGAA
    GAGAAGAAAG AGGAGGAGGA AGACGACCCC GGGGAAGACG GCCCCAAGCC CATGCCCCCC
    TACAGCTCCA TGTTTATCCT CTCTACGACC AACCCCCTTC GCCGCCTGTG CCATTACATC
    CTGAACCTGC GCTACTTTGA GATGTGCATT CTCATGGTCA TCGCCATGAG CAGCATCGCC
    CTGGCGGCCG AGGATCCTGT TCAGCCCAAC GCACCACGAA ACAATGTGCT GCGATATTTT
    GACTACGTTT TTACAGGCGT CTTTACCTTT GAGATGGTGA TCAAGATGAT CGACCTGGGG
    CTCGTCCTGC ACCAGGGTGC CTACTTCCGT GACCTCTGGA ACATTCTCGA CTTCATAGTG
    GTCAGTGGGG CCCTGGTGGC CTTTGCCTTC ACTGGCAATA GCAAAGGAAA GGACATCAAC
    ACGATTAAAT CCCTCCGAGT CCTCCGGGTG CTACGACCTC TTAAAACCAT CAAGCGGTTG
    CCAAAGCTCA AGGCCGTGTT TGACTGCGTG GTGAACTCTC TCAAGAATGT CTTCAACATC
    CTCATCGTCT ACATGCTGTT CATGTTCATC TTCGCCGTGG TGGCCGTGCA GCTCTTCAAG
    GGCAAGTTTT TCCACTGCAC AGATGAGTCC AAGGAGTTTG AGAAAGATTG CCGCGGCAAG
    TACCTCCTCT ATGAGAAGAA TGAGGTAAAG GCGAGGGATC GAGAGTGGAA GAAGTATGAG
    TTCCATTATG ACAATGTGCT GTGGGCGCTG CTGACCCTCT TCACCGTGTC CACGGGAGAA
    GGCTGGCCAC AGGTCCTCAA GCACTCCGTG GACGCCACCT TCGAGAACCA GGGCCCCAGC
    CCCGGGTACC GCATGGAGAT GTCCATCTTC TACGTCGTCT ACTTTGTGGT GTTCCCCTTC
    TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATCATCACCT TCCAGGAGCA GGGGGACAAG
    ATGATGGAGG AGTACAGCCT GGAGAAAAAT GAGAGGGCGT GCATCGACTT CGCCATCAGC
    GCCAAACCCC TGACCCGGCA CATGCCGCAG AACAAGCAGA GTTTCCAGTA CCGCATGTGG
    CAGTTCGTGG TGTCCCCGCC TTTCGAGTAC ACCATCATGG CCATGATCGC CCTCAACACC
    ATCGTGCTCA TGATGAAGTT CTACGGGGCC TCTGTTGCTT ATGAGAATGC TCTGAGAGTA
    TTCAACATCG TCTTCACCTC CCTCTTCTCA CTGGAATGTG TGCTGAAAGT CATGGCTTTT
    GGGATTCTGA ATTATTTTCG CGATGCCTGG AACATCTTCG ACTTTGTGAC TGTTCTGGGC
    AGCATCACCG ACATCCTCGT GACCGAGTTT GGGAATAACT TCATCAACTT GAGCTTCCTC
    CGCCTCTTCC GAGCTGCCCG GCTCATCAAA CTCCTCCGTC AGGGTTACAC CATCCGAATT
    CTTCTCTGGA CCTTTGTGCA GTCCTTCAAG GCCCTGCCCT ATGTCTGTCT ACTGATTGCC
    ATGCTTTTTT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGCAACAT TGGCATCGAT
    GTGGAGGACG AGGACAGTGA TGAAGATGAA TTCCAAATCA CTGAGCACAA TAACTTCCGC
    ACCTTCTTCC AGGCCCTCAT GCTTCTCTTC CGGAGTGCCA CTGGGGAGGC TTGGCACAAC
    ATCATGCTTT CCTGCCTCAG CGGGAAACCC TGTGATAAGA ACTCTGGCAT CCTGACTCAC
    GAATGCGGCA ATGAATTTGC TTATTTTTAC TTCGTTTCCT TCATCTTCCT CTGCTCGTTT
    CTGATGCTGA ATCTTTTTGT TGCCGTCATC ATGGACAACT TTGAGTACCT CACCCGAGAT
    TCCTCCATCC TGGGTCCCCA CCACCTGGAT GAGTACGTGC GTGTCTGGGC CGAGTATGAC
    CCTGCAGCGT GGGGCCGCAT GCCTTACCCG GACATGTATC AGATGCTGAG ACACATGTCT
    CCGCCCCTGG GTCTGGGGAA GAAGTGTCCG GCCAGAGTGG CTTACAAGAG GCTCCTGCGG
    ATGGATCTGC CCGTCGCTGA TGACAACACC GTCCACTTCA ACTCTACCCT CATGGCTCTG
    ATCCGCACGG CCCTGGACAT CAAAATTGCC AAAGGTGGAG CTGACAAACA ACAAATGGAT
    GCTGAGTTGC GGAAGGAGAT GATGGCCATC TGGCCCAATC TGTCCCAGAA GACGCTGGAC
    CTGCTGGTCA CCCCCCACAA GTCCACGGAC CTGACAGTGG GGAAGATCTA TGCGGCCATG
    ATGATCATGG AGTATTACCG GCAGAGCAAG GCCAAGAAGC TTCAGGCCAT GCGCGAGGAA
    CAGAACCGGA CACCTCTCAT GTTCCAGCGC ATGGAGCCCC CATCGCCAAC ACAGGAGGGG
    GGGCCTGGCC AGAACGCCCT CCCCTCCACC CAGCTGGACC CAGGAGGAGG CTTGATGGCT
    CACGAAAGTG GCATGAAAGA GAGCCCATCC TGGGTGACCC AGCGGGCCCA GGAGATGTTC
    CAGAAGACGG GCACATGGAG CCCAGAGCGG GGCCCCCCCA CTGATATGCC TAATAGCCAA
    CCCAACTCTC AGTCTGTGGA GATGCGAGAG ATGGGCAGAG ACGGCTACTC TGACAGTGAG
    CACTACTTCC CCGTTGAAGG CCAGGCCCGG GCCGCCTCCA TGCCCCGCCT GCCTGCCGAG
    AACCAGAGGA GAAGGGGCCG GCCACGTGGG AATAACCTCA GTACCATCTC AGACACCAGC
    CCCATGAAGC GCTCGGCCTC CGTGCTGGGC CCCAAGGCCC GGCGCCTGGA CGACTACTCC
    CTGGAGAGGG TGCCGCCCGA GGAGAACCAG CGGCACCACC AGAGGCGCAG AGACCGTGGC
    CACCGCACCT CCGAGCGCTC CTTGGGCCGC TACACGGACG TGGACACAGG CTTGGGGACA
    GACTTGAGCA TGACCACCCA GTCTGGGGAC CTGCCATCAA AGGAAAGAGA CCAGGAGCGG
    GGCCGGCCCA AGGACCGGAA GCATCGGCAA CACCACCACC ACCACCACCA CCACCATCCC
    CCGCCCCCTG ACAAGGAGCG CTACACCCAG GAGCGGCCTG AGCACGGCCG GCCCCGGGCC
    CGTGACCAGC GCTGGTCCCG CTCACCCAGT GAGGGCCGGG AGCACATGGC CCACCGTCAG
    GGCAGTAGTT CCGTAAGTGG AAGCCCAGCC CCCTCAACAT CTGGTACCAG CACTCCGCGG
    CGGGGACGCC GCCAGCTCCC CCAGACCCCC TCCACTCCCC GGCCGCACGT GTCCTATTCC
    CCCGTGATCC GTAAGGCCGG CGGCTCGGGT CCCCAGCAGC AGCAGCAGCC CGCGGCGCGG
    CCGGGCCGAA CGGCCCCCAG CGGCCCGCGG AGGTACCCGG GCCCCGTGGC CGAGCCCCTG
    GCCGGGGAGC GGCCTCCCAC GGGAGGCCAC GGAAGCAGCC GCTCGCCCGC GATGGAGCGG
    CGGATCCCGG GCCCGGCCCG GAGCGAGTCC CCCAGGGCGC CGTGTCGCCA CGGCGGGGCG
    CGATGGCCGG CGTCCGGCGC GCACGCGGCC GAGGGCCCCC CGGGCCCCCG GCACCACCAC
    GGTTACTACC GGGGCTCCGA CTACGACGAG CCAGACGGCC CGGGCGGCGG CGGCGGGGAG
    GAGGAGGAGC CCCGGGCCGC CGCCTACGAC GCGCCGCCCC CCGCGCGCCA CGGGTGCTCG
    CCCAGGACTC CCCGGGCCGC GGGCCCGGCC TGCGCCTCGC CTTCCAGGCA CGGCCGGCGA
    CTCCCGAACG GCTACTACCC GGCCCACGGA CTGGCCAGGC CCCGCGGGCC GGGCTCCCGG
    AAAGGCCTGC ACGAAGCCTA CAGCGAGACC GACGACGATG ACTGGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235776
    Clone ID Related Accession (Same CDS sequence) XM_024993854.1
    Accession Version XM_024993854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA
    TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC
    GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG
    GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC
    TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG
    TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG
    CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC
    TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC
    TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA
    AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC
    CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC
    GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC
    GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG
    GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG
    AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG
    CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC
    TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC
    GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG
    GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG
    GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC
    AGCGAGACCG ACGACGATGA CTGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849622.1
    CDS230..7699
    Translation

    Target ORF information:

    RefSeq Version XM_024993854.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA
    TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC
    GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG
    GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC
    TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG
    TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG
    CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC
    TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC
    TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA
    AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC
    CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC
    GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC
    GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG
    GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG
    AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG
    CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC
    TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC
    GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG
    GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG
    GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC
    AGCGAGACCG ACGACGATGA CTGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235777
    Clone ID Related Accession (Same CDS sequence) XM_024993855.1
    Accession Version XM_024993855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC
    GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG
    GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC
    TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG
    TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG
    CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC
    TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC
    TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA
    AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC
    CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC
    GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC
    GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG
    GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG
    AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG
    CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC
    TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC
    GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG
    GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG
    GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC
    AGCGAGACCG ACGACGATGA CTGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849623.1
    CDS230..7699
    Translation

    Target ORF information:

    RefSeq Version XM_024993855.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGGGGC CGCATGCCTT ACCCGGACAT GTATCAGATG
    CTGAGACACA TGTCTCCGCC CCTGGGTCTG GGGAAGAAGT GTCCGGCCAG AGTGGCTTAC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GACCATCTCA GACACCAGCC CCATGAAGCG CTCGGCCTCC
    GTGCTGGGCC CCAAGGCCCG GCGCCTGGAC GACTACTCCC TGGAGAGGGT GCCGCCCGAG
    GAGAACCAGC GGCACCACCA GAGGCGCAGA GACCGTGGCC ACCGCACCTC CGAGCGCTCC
    TTGGGCCGCT ACACGGACGT GGACACAGGC TTGGGGACAG ACTTGAGCAT GACCACCCAG
    TCTGGGGACC TGCCATCAAA GGAAAGAGAC CAGGAGCGGG GCCGGCCCAA GGACCGGAAG
    CATCGGCAAC ACCACCACCA CCACCACCAC CACCATCCCC CGCCCCCTGA CAAGGAGCGC
    TACACCCAGG AGCGGCCTGA GCACGGCCGG CCCCGGGCCC GTGACCAGCG CTGGTCCCGC
    TCACCCAGTG AGGGCCGGGA GCACATGGCC CACCGTCAGG GCAGTAGTTC CGTAAGTGGA
    AGCCCAGCCC CCTCAACATC TGGTACCAGC ACTCCGCGGC GGGGACGCCG CCAGCTCCCC
    CAGACCCCCT CCACTCCCCG GCCGCACGTG TCCTATTCCC CCGTGATCCG TAAGGCCGGC
    GGCTCGGGTC CCCAGCAGCA GCAGCAGCCC GCGGCGCGGC CGGGCCGAAC GGCCCCCAGC
    GGCCCGCGGA GGTACCCGGG CCCCGTGGCC GAGCCCCTGG CCGGGGAGCG GCCTCCCACG
    GGAGGCCACG GAAGCAGCCG CTCGCCCGCG ATGGAGCGGC GGATCCCGGG CCCGGCCCGG
    AGCGAGTCCC CCAGGGCGCC GTGTCGCCAC GGCGGGGCGC GATGGCCGGC GTCCGGCGCG
    CACGCGGCCG AGGGCCCCCC GGGCCCCCGG CACCACCACG GTTACTACCG GGGCTCCGAC
    TACGACGAGC CAGACGGCCC GGGCGGCGGC GGCGGGGAGG AGGAGGAGCC CCGGGCCGCC
    GCCTACGACG CGCCGCCCCC CGCGCGCCAC GGGTGCTCGC CCAGGACTCC CCGGGCCGCG
    GGCCCGGCCT GCGCCTCGCC TTCCAGGCAC GGCCGGCGAC TCCCGAACGG CTACTACCCG
    GCCCACGGAC TGGCCAGGCC CCGCGGGCCG GGCTCCCGGA AAGGCCTGCA CGAAGCCTAC
    AGCGAGACCG ACGACGATGA CTGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235778
    Clone ID Related Accession (Same CDS sequence) XM_024993856.1
    Accession Version XM_024993856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATAAC TTCATCAACT TGAGCTTCCT CCGCCTCTTC
    CGAGCTGCCC GGCTCATCAA ACTCCTCCGT CAGGGTTACA CCATCCGAAT TCTTCTCTGG
    ACCTTTGTGC AGTCCTTCAA GGCCCTGCCC TATGTCTGTC TACTGATTGC CATGCTTTTT
    TTCATCTACG CCATCATTGG GATGCAGGTG TTTGGCAACA TTGGCATCGA TGTGGAGGAC
    GAGGACAGTG ATGAAGATGA ATTCCAAATC ACTGAGCACA ATAACTTCCG CACCTTCTTC
    CAGGCCCTCA TGCTTCTCTT CCGGAGTGCC ACTGGGGAGG CTTGGCACAA CATCATGCTT
    TCCTGCCTCA GCGGGAAACC CTGTGATAAG AACTCTGGCA TCCTGACTCA CGAATGCGGC
    AATGAATTTG CTTATTTTTA CTTCGTTTCC TTCATCTTCC TCTGCTCGTT TCTGATGCTG
    AATCTTTTTG TTGCCGTCAT CATGGACAAC TTTGAGTACC TCACCCGAGA TTCCTCCATC
    CTGGGTCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CCGAGTATGA CCCTGCAGCG
    TGCGGTCGGA TTCATTATAA GGATATGTAC AGTTTATTGC GCGTAATATC TCCCCCTCTC
    GGCTTAGGCA AGAAATGTCC TCATAGGGTT GCTTGCAAGA GGCTCCTGCG GATGGATCTG
    CCCGTCGCTG ATGACAACAC CGTCCACTTC AACTCTACCC TCATGGCTCT GATCCGCACG
    GCCCTGGACA TCAAAATTGC CAAAGGTGGA GCTGACAAAC AACAAATGGA TGCTGAGTTG
    CGGAAGGAGA TGATGGCCAT CTGGCCCAAT CTGTCCCAGA AGACGCTGGA CCTGCTGGTC
    ACCCCCCACA AGTCCACGGA CCTGACAGTG GGGAAGATCT ATGCGGCCAT GATGATCATG
    GAGTATTACC GGCAGAGCAA GGCCAAGAAG CTTCAGGCCA TGCGCGAGGA ACAGAACCGG
    ACACCTCTCA TGTTCCAGCG CATGGAGCCC CCATCGCCAA CACAGGAGGG GGGGCCTGGC
    CAGAACGCCC TCCCCTCCAC CCAGCTGGAC CCAGGAGGAG GCTTGATGGC TCACGAAAGT
    GGCATGAAAG AGAGCCCATC CTGGGTGACC CAGCGGGCCC AGGAGATGTT CCAGAAGACG
    GGCACATGGA GCCCAGAGCG GGGCCCCCCC ACTGATATGC CTAATAGCCA ACCCAACTCT
    CAGTCTGTGG AGATGCGAGA GATGGGCAGA GACGGCTACT CTGACAGTGA GCACTACTTC
    CCCGTTGAAG GCCAGGCCCG GGCCGCCTCC ATGCCCCGCC TGCCTGCCGA GAACCAGAGG
    AGAAGGGGCC GGCCACGTGG GAATAACCTC AGTACCATCT CAGACACCAG CCCCATGAAG
    CGCTCGGCCT CCGTGCTGGG CCCCAAGGCC CGGCGCCTGG ACGACTACTC CCTGGAGAGG
    GTGCCGCCCG AGGAGAACCA GCGGCACCAC CAGAGGCGCA GAGACCGTGG CCACCGCACC
    TCCGAGCGCT CCTTGGGCCG CTACACGGAC GTGGACACAG GCTTGGGGAC AGACTTGAGC
    ATGACCACCC AGTCTGGGGA CCTGCCATCA AAGGAAAGAG ACCAGGAGCG GGGCCGGCCC
    AAGGACCGGA AGCATCGGCA ACACCACCAC CACCACCACC ACCACCATCC CCCGCCCCCT
    GACAAGGAGC GCTACACCCA GGAGCGGCCT GAGCACGGCC GGCCCCGGGC CCGTGACCAG
    CGCTGGTCCC GCTCACCCAG TGAGGGCCGG GAGCACATGG CCCACCGTCA GGGCAGTAGT
    TCCGTAAGTG GAAGCCCAGC CCCCTCAACA TCTGGTACCA GCACTCCGCG GCGGGGACGC
    CGCCAGCTCC CCCAGACCCC CTCCACTCCC CGGCCGCACG TGTCCTATTC CCCCGTGATC
    CGTAAGGCCG GCGGCTCGGG TCCCCAGCAG CAGCAGCAGC CCGCGGCGCG GCCGGGCCGA
    ACGGCCCCCA GCGGCCCGCG GAGGTACCCG GGCCCCGTGG CCGAGCCCCT GGCCGGGGAG
    CGGCCTCCCA CGGGAGGCCA CGGAAGCAGC CGCTCGCCCG CGATGGAGCG GCGGATCCCG
    GGCCCGGCCC GGAGCGAGTC CCCCAGGGCG CCGTGTCGCC ACGGCGGGGC GCGATGGCCG
    GCGTCCGGCG CGCACGCGGC CGAGGGCCCC CCGGGCCCCC GGCACCACCA CGGTTACTAC
    CGGGGCTCCG ACTACGACGA GCCAGACGGC CCGGGCGGCG GCGGCGGGGA GGAGGAGGAG
    CCCCGGGCCG CCGCCTACGA CGCGCCGCCC CCCGCGCGCC ACGGGTGCTC GCCCAGGACT
    CCCCGGGCCG CGGGCCCGGC CTGCGCCTCG CCTTCCAGGC ACGGCCGGCG ACTCCCGAAC
    GGCTACTACC CGGCCCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCCG GAAAGGCCTG
    CACGAAGCCT ACAGCGAGAC CGACGACGAT GACTGGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849624.1
    CDS231..7652
    Translation

    Target ORF information:

    RefSeq Version XM_024993856.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATAAC TTCATCAACT TGAGCTTCCT CCGCCTCTTC
    CGAGCTGCCC GGCTCATCAA ACTCCTCCGT CAGGGTTACA CCATCCGAAT TCTTCTCTGG
    ACCTTTGTGC AGTCCTTCAA GGCCCTGCCC TATGTCTGTC TACTGATTGC CATGCTTTTT
    TTCATCTACG CCATCATTGG GATGCAGGTG TTTGGCAACA TTGGCATCGA TGTGGAGGAC
    GAGGACAGTG ATGAAGATGA ATTCCAAATC ACTGAGCACA ATAACTTCCG CACCTTCTTC
    CAGGCCCTCA TGCTTCTCTT CCGGAGTGCC ACTGGGGAGG CTTGGCACAA CATCATGCTT
    TCCTGCCTCA GCGGGAAACC CTGTGATAAG AACTCTGGCA TCCTGACTCA CGAATGCGGC
    AATGAATTTG CTTATTTTTA CTTCGTTTCC TTCATCTTCC TCTGCTCGTT TCTGATGCTG
    AATCTTTTTG TTGCCGTCAT CATGGACAAC TTTGAGTACC TCACCCGAGA TTCCTCCATC
    CTGGGTCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CCGAGTATGA CCCTGCAGCG
    TGCGGTCGGA TTCATTATAA GGATATGTAC AGTTTATTGC GCGTAATATC TCCCCCTCTC
    GGCTTAGGCA AGAAATGTCC TCATAGGGTT GCTTGCAAGA GGCTCCTGCG GATGGATCTG
    CCCGTCGCTG ATGACAACAC CGTCCACTTC AACTCTACCC TCATGGCTCT GATCCGCACG
    GCCCTGGACA TCAAAATTGC CAAAGGTGGA GCTGACAAAC AACAAATGGA TGCTGAGTTG
    CGGAAGGAGA TGATGGCCAT CTGGCCCAAT CTGTCCCAGA AGACGCTGGA CCTGCTGGTC
    ACCCCCCACA AGTCCACGGA CCTGACAGTG GGGAAGATCT ATGCGGCCAT GATGATCATG
    GAGTATTACC GGCAGAGCAA GGCCAAGAAG CTTCAGGCCA TGCGCGAGGA ACAGAACCGG
    ACACCTCTCA TGTTCCAGCG CATGGAGCCC CCATCGCCAA CACAGGAGGG GGGGCCTGGC
    CAGAACGCCC TCCCCTCCAC CCAGCTGGAC CCAGGAGGAG GCTTGATGGC TCACGAAAGT
    GGCATGAAAG AGAGCCCATC CTGGGTGACC CAGCGGGCCC AGGAGATGTT CCAGAAGACG
    GGCACATGGA GCCCAGAGCG GGGCCCCCCC ACTGATATGC CTAATAGCCA ACCCAACTCT
    CAGTCTGTGG AGATGCGAGA GATGGGCAGA GACGGCTACT CTGACAGTGA GCACTACTTC
    CCCGTTGAAG GCCAGGCCCG GGCCGCCTCC ATGCCCCGCC TGCCTGCCGA GAACCAGAGG
    AGAAGGGGCC GGCCACGTGG GAATAACCTC AGTACCATCT CAGACACCAG CCCCATGAAG
    CGCTCGGCCT CCGTGCTGGG CCCCAAGGCC CGGCGCCTGG ACGACTACTC CCTGGAGAGG
    GTGCCGCCCG AGGAGAACCA GCGGCACCAC CAGAGGCGCA GAGACCGTGG CCACCGCACC
    TCCGAGCGCT CCTTGGGCCG CTACACGGAC GTGGACACAG GCTTGGGGAC AGACTTGAGC
    ATGACCACCC AGTCTGGGGA CCTGCCATCA AAGGAAAGAG ACCAGGAGCG GGGCCGGCCC
    AAGGACCGGA AGCATCGGCA ACACCACCAC CACCACCACC ACCACCATCC CCCGCCCCCT
    GACAAGGAGC GCTACACCCA GGAGCGGCCT GAGCACGGCC GGCCCCGGGC CCGTGACCAG
    CGCTGGTCCC GCTCACCCAG TGAGGGCCGG GAGCACATGG CCCACCGTCA GGGCAGTAGT
    TCCGTAAGTG GAAGCCCAGC CCCCTCAACA TCTGGTACCA GCACTCCGCG GCGGGGACGC
    CGCCAGCTCC CCCAGACCCC CTCCACTCCC CGGCCGCACG TGTCCTATTC CCCCGTGATC
    CGTAAGGCCG GCGGCTCGGG TCCCCAGCAG CAGCAGCAGC CCGCGGCGCG GCCGGGCCGA
    ACGGCCCCCA GCGGCCCGCG GAGGTACCCG GGCCCCGTGG CCGAGCCCCT GGCCGGGGAG
    CGGCCTCCCA CGGGAGGCCA CGGAAGCAGC CGCTCGCCCG CGATGGAGCG GCGGATCCCG
    GGCCCGGCCC GGAGCGAGTC CCCCAGGGCG CCGTGTCGCC ACGGCGGGGC GCGATGGCCG
    GCGTCCGGCG CGCACGCGGC CGAGGGCCCC CCGGGCCCCC GGCACCACCA CGGTTACTAC
    CGGGGCTCCG ACTACGACGA GCCAGACGGC CCGGGCGGCG GCGGCGGGGA GGAGGAGGAG
    CCCCGGGCCG CCGCCTACGA CGCGCCGCCC CCCGCGCGCC ACGGGTGCTC GCCCAGGACT
    CCCCGGGCCG CGGGCCCGGC CTGCGCCTCG CCTTCCAGGC ACGGCCGGCG ACTCCCGAAC
    GGCTACTACC CGGCCCACGG ACTGGCCAGG CCCCGCGGGC CGGGCTCCCG GAAAGGCCTG
    CACGAAGCCT ACAGCGAGAC CGACGACGAT GACTGGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235779
    Clone ID Related Accession (Same CDS sequence) XM_024993858.1
    Accession Version XM_024993858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGAC TATGCAGAAT TCATTTTCTT AGGACTCTTT
    ATGTCCGAAA TGTTTATAAA AATGTACGGG CTTGGGACGC GGCCTTACTT CCACTCTTCC
    TTCAACTGTT TTGATTGTGG GGTCATCATC GGGAGTATCT TTGAGGTCAT CTGGGCTGTC
    ATAAAACCCG GCACATCCTT TGGAATCAGT GTGTTACGAG CCCTCAGGTT ATTGCGTATT
    TTCAAAGTCA CAAAGTACTG GGCATCTCTC AGGAACCTGG TCGTCTCTCT TCTCAACTCC
    ATGAAATCCA TCATCAGCCT GTTGTTCCTC CTTTTCCTAT TCATCGTCGT CTTTGCCCTT
    CTGGGAATGC AACTCTTTGG CGGCCAGTTT AATTTCGATG AAGGGACTCC TCCTACCAAC
    TTCGACACTT TTCCAGCAGC AATAATGACA GTTTTTCAGA TCCTGACGGG CGAAGACTGG
    AACGAGGTCA TGTATGACGG CATCAAGTCT CAGGGGGGCG TGCAAGGCGG CATGGTGTTC
    TCCATCTACT TCATCGTGCT GACGCTCTTC GGGAACTACA CCCTCCTGAA CGTGTTCTTG
    GCCATCGCGG TGGATAATCT GGCTAACGCC CAGGAGCTCA CCAAGGATGA ACAAGAAGAA
    GAAGAGGCTG CCAACCAGAA ACTCGCCCTC CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT
    CCTCTGTCTG CAGCCAACAT GTCCATAGCT GTGAAGGAGC AACAGAAGAA CCAGAAGCCC
    GCCAAGTCGG TGTGGGAGCA GCGGACAAGC GAGATGAGGA AGCAGAATCT TCTGGCCAGT
    CGGGAGGCAC TCTACAACGA GATGGACCCA GACGAGCGCT GGAAGGCCTC CTACGCTCGC
    CACCTGCGGC CCGACATGAA GACCCACCTG GACCGGCCGC TGGTGGTCGA CCCCCAGGAG
    AACCGTAACA ACAACACCAA CAAGAGCCGG GCGGCCGAGC CCACGGTGGA CCAGCGCCTC
    GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG AAACAGGCCC GCTACCACGA CCGGGCCCGG
    GACCCCAGCA GCTCCGTGGG CCTGGACACC CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC
    GAGCTGAGCC GGGAGGGGCC TTACGGTCGC GAGTCGGACC ACCACGCCCG GGAGGGCGGC
    CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG GCGGAGCGGG GCAAGGCGGG CGACCCGCAC
    CGGAGGCACG CGCACCGGCA GGCGGGCAGC CGCGAGAGCC GCAGCGGGTC CCCGCGCACC
    GGGGCCGACG GAGAGCCCCG CCGGCACCGG GCGCACCGCA GGCCGGGCGA CGAGGCCCCG
    GACGACAAGG CCGATCGGAG GTCCCGGCAC CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC
    GAGGGCGAGA GCGAGGGCCC AGACGGCGGC GGCGAGCGGA GGCGGCGGCA CCGGCATGGC
    CCTCCCCCAG CGTACGACGC GGACATGCGC AGGGAGGACA AGGAGCGCAG GCACCGCAGG
    AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC GTGTCAGGCC CCAACCTGTC GACCACACGG
    CCAATCCAGC AGGACCTGGG CCGCCAAGAC CCCCCGCTGG CCGAGGATAT CGACAACATG
    AAGAACAACA AACTGGCCAC TGCGGAGTCA GCCAGTCCCC ACGACAGCCT CAGCCACGCC
    GGCCTGCCCC AGAGCCCAGC CAAGATGGGG AACAGCATGG ACCCTGGCCC CACACCGGCC
    CCCACCACCA CGGCCACCAA CCCTCAGAAT GCCGTCAGTC GCCGGACAGC CAACAACCCG
    GGGAACCCGT CCAACCCCGG CCCCCCCAAG ACCCCTGAGA ACAGCCTTAT CGTCACCAAC
    CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG ACTGCCAGGA AACCTGACCA CACCACGGTG
    GACATCCCCC CAGCCTGCCC ACCCCCACTC AACCACACCA TCATCCAAGT GAACAAAAAC
    GCCAACCCAG ACCCACTGCC AAAAAAGGAG GAAGAGAAGA AAGAGGAGGA GGAAGACGAC
    CCCGGGGAAG ACGGCCCCAA GCCCATGCCC CCCTACAGCT CCATGTTTAT CCTCTCTACG
    ACCAACCCCC TTCGCCGCCT GTGCCATTAC ATCCTGAACC TGCGCTACTT TGAGATGTGC
    ATTCTCATGG TCATCGCCAT GAGCAGCATC GCCCTGGCGG CCGAGGATCC TGTTCAGCCC
    AACGCACCAC GAAACAATGT GCTGCGATAT TTTGACTACG TTTTTACAGG CGTCTTTACC
    TTTGAGATGG TGATCAAGAT GATCGACCTG GGGCTCGTCC TGCACCAGGG TGCCTACTTC
    CGTGACCTCT GGAACATTCT CGACTTCATA GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC
    TTCACGAGCA GTACACGTGG CAATAGCAAA GGAAAGGACA TCAACACGAT TAAATCCCTC
    CGAGTCCTCC GGGTGCTACG ACCTCTTAAA ACCATCAAGC GGTTGCCAAA GCTCAAGGCC
    GTGTTTGACT GCGTGGTGAA CTCTCTCAAG AATGTCTTCA ACATCCTCAT CGTCTACATG
    CTGTTCATGT TCATCTTCGC CGTGGTGGCC GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC
    TGCACAGATG AGTCCAAGGA GTTTGAGAAA GATTGCCGCG GCAAGTACCT CCTCTATGAG
    AAGAATGAGG TAAAGGCGAG GGATCGAGAG TGGAAGAAGT ATGAGTTCCA TTATGACAAT
    GTGCTGTGGG CGCTGCTGAC CCTCTTCACC GTGTCCACGG GAGAAGGCTG GCCACAGGTC
    CTCAAGCACT CCGTGGACGC CACCTTCGAG AACCAGGGCC CCAGCCCCGG GTACCGCATG
    GAGATGTCCA TCTTCTACGT CGTCTACTTT GTGGTGTTCC CCTTCTTCTT TGTCAACATC
    TTTGTGGCCT TGATCATCAT CACCTTCCAG GAGCAGGGGG ACAAGATGAT GGAGGAGTAC
    AGCCTGGAGA AAAATGAGAG GGCGTGCATC GACTTCGCCA TCAGCGCCAA ACCCCTGACC
    CGGCACATGC CGCAGAACAA GCAGAGTTTC CAGTACCGCA TGTGGCAGTT CGTGGTGTCC
    CCGCCTTTCG AGTACACCAT CATGGCCATG ATCGCCCTCA ACACCATCGT GCTCATGATG
    AAGTTCTACG GGGCCTCTGT TGCTTATGAG AATGCTCTGA GAGTATTCAA CATCGTCTTC
    ACCTCCCTCT TCTCACTGGA ATGTGTGCTG AAAGTCATGG CTTTTGGGAT TCTGAATTAT
    TTTCGCGATG CCTGGAACAT CTTCGACTTT GTGACTGTTC TGGGCAGCAT CACCGACATC
    CTCGTGACCG AGTTTGGGAA TAACTTCATC AACTTGAGCT TCCTCCGCCT CTTCCGAGCT
    GCCCGGCTCA TCAAACTCCT CCGTCAGGGT TACACCATCC GAATTCTTCT CTGGACCTTT
    GTGCAGTCCT TCAAGGCCCT GCCCTATGTC TGTCTACTGA TTGCCATGCT TTTTTTCATC
    TACGCCATCA TTGGGATGCA GGTGTTTGGC AACATTGGCA TCGATGTGGA GGACGAGGAC
    AGTGATGAAG ATGAATTCCA AATCACTGAG CACAATAACT TCCGCACCTT CTTCCAGGCC
    CTCATGCTTC TCTTCCGGAG TGCCACTGGG GAGGCTTGGC ACAACATCAT GCTTTCCTGC
    CTCAGCGGGA AACCCTGTGA TAAGAACTCT GGCATCCTGA CTCACGAATG CGGCAATGAA
    TTTGCTTATT TTTACTTCGT TTCCTTCATC TTCCTCTGCT CGTTTCTGAT GCTGAATCTT
    TTTGTTGCCG TCATCATGGA CAACTTTGAG TACCTCACCC GAGATTCCTC CATCCTGGGT
    CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCCGAGT ATGACCCTGC AGCGTGCGGT
    CGGATTCATT ATAAGGATAT GTACAGTTTA TTGCGCGTAA TATCTCCCCC TCTCGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCC TGCGGATGGA TCTGCCCGTC
    GCTGATGACA ACACCGTCCA CTTCAACTCT ACCCTCATGG CTCTGATCCG CACGGCCCTG
    GACATCAAAA TTGCCAAAGG TGGAGCTGAC AAACAACAAA TGGATGCTGA GTTGCGGAAG
    GAGATGATGG CCATCTGGCC CAATCTGTCC CAGAAGACGC TGGACCTGCT GGTCACCCCC
    CACAAGTCCA CGGACCTGAC AGTGGGGAAG ATCTATGCGG CCATGATGAT CATGGAGTAT
    TACCGGCAGA GCAAGGCCAA GAAGCTTCAG GCCATGCGCG AGGAACAGAA CCGGACACCT
    CTCATGTTCC AGCGCATGGA GCCCCCATCG CCAACACAGG AGGGGGGGCC TGGCCAGAAC
    GCCCTCCCCT CCACCCAGCT GGACCCAGGA GGAGGCTTGA TGGCTCACGA AAGTGGCATG
    AAAGAGAGCC CATCCTGGGT GACCCAGCGG GCCCAGGAGA TGTTCCAGAA GACGGGCACA
    TGGAGCCCAG AGCGGGGCCC CCCCACTGAT ATGCCTAATA GCCAACCCAA CTCTCAGTCT
    GTGGAGATGC GAGAGATGGG CAGAGACGGC TACTCTGACA GTGAGCACTA CTTCCCCGTT
    GAAGGCCAGG CCCGGGCCGC CTCCATGCCC CGCCTGCCTG CCGAGAACCA GAGGAGAAGG
    GGCCGGCCAC GTGGGAATAA CCTCAGTACC ATCTCAGACA CCAGCCCCAT GAAGCGCTCG
    GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC CTGGACGACT ACTCCCTGGA GAGGGTGCCG
    CCCGAGGAGA ACCAGCGGCA CCACCAGAGG CGCAGAGACC GTGGCCACCG CACCTCCGAG
    CGCTCCTTGG GCCGCTACAC GGACGTGGAC ACAGGCTTGG GGACAGACTT GAGCATGACC
    ACCCAGTCTG GGGACCTGCC ATCAAAGGAA AGAGACCAGG AGCGGGGCCG GCCCAAGGAC
    CGGAAGCATC GGCAACACCA CCACCACCAC CACCACCACC ATCCCCCGCC CCCTGACAAG
    GAGCGCTACA CCCAGGAGCG GCCTGAGCAC GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG
    TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC ATGGCCCACC GTCAGGGCAG TAGTTCCGTA
    AGTGGAAGCC CAGCCCCCTC AACATCTGGT ACCAGCACTC CGCGGCGGGG ACGCCGCCAG
    CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG CACGTGTCCT ATTCCCCCGT GATCCGTAAG
    GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG CAGCCCGCGG CGCGGCCGGG CCGAACGGCC
    CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT
    CCCACGGGAG GCCACGGAAG CAGCCGCTCG CCCGCGATGG AGCGGCGGAT CCCGGGCCCG
    GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT CGCCACGGCG GGGCGCGATG GCCGGCGTCC
    GGCGCGCACG CGGCCGAGGG CCCCCCGGGC CCCCGGCACC ACCACGGTTA CTACCGGGGC
    TCCGACTACG ACGAGCCAGA CGGCCCGGGC GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG
    GCCGCCGCCT ACGACGCGCC GCCCCCCGCG CGCCACGGGT GCTCGCCCAG GACTCCCCGG
    GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC AGGCACGGCC GGCGACTCCC GAACGGCTAC
    TACCCGGCCC ACGGACTGGC CAGGCCCCGC GGGCCGGGCT CCCGGAAAGG CCTGCACGAA
    GCCTACAGCG AGACCGACGA CGATGACTGG TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849626.1
    CDS230..7525
    Translation

    Target ORF information:

    RefSeq Version XM_024993858.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGAC TATGCAGAAT TCATTTTCTT AGGACTCTTT
    ATGTCCGAAA TGTTTATAAA AATGTACGGG CTTGGGACGC GGCCTTACTT CCACTCTTCC
    TTCAACTGTT TTGATTGTGG GGTCATCATC GGGAGTATCT TTGAGGTCAT CTGGGCTGTC
    ATAAAACCCG GCACATCCTT TGGAATCAGT GTGTTACGAG CCCTCAGGTT ATTGCGTATT
    TTCAAAGTCA CAAAGTACTG GGCATCTCTC AGGAACCTGG TCGTCTCTCT TCTCAACTCC
    ATGAAATCCA TCATCAGCCT GTTGTTCCTC CTTTTCCTAT TCATCGTCGT CTTTGCCCTT
    CTGGGAATGC AACTCTTTGG CGGCCAGTTT AATTTCGATG AAGGGACTCC TCCTACCAAC
    TTCGACACTT TTCCAGCAGC AATAATGACA GTTTTTCAGA TCCTGACGGG CGAAGACTGG
    AACGAGGTCA TGTATGACGG CATCAAGTCT CAGGGGGGCG TGCAAGGCGG CATGGTGTTC
    TCCATCTACT TCATCGTGCT GACGCTCTTC GGGAACTACA CCCTCCTGAA CGTGTTCTTG
    GCCATCGCGG TGGATAATCT GGCTAACGCC CAGGAGCTCA CCAAGGATGA ACAAGAAGAA
    GAAGAGGCTG CCAACCAGAA ACTCGCCCTC CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT
    CCTCTGTCTG CAGCCAACAT GTCCATAGCT GTGAAGGAGC AACAGAAGAA CCAGAAGCCC
    GCCAAGTCGG TGTGGGAGCA GCGGACAAGC GAGATGAGGA AGCAGAATCT TCTGGCCAGT
    CGGGAGGCAC TCTACAACGA GATGGACCCA GACGAGCGCT GGAAGGCCTC CTACGCTCGC
    CACCTGCGGC CCGACATGAA GACCCACCTG GACCGGCCGC TGGTGGTCGA CCCCCAGGAG
    AACCGTAACA ACAACACCAA CAAGAGCCGG GCGGCCGAGC CCACGGTGGA CCAGCGCCTC
    GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG AAACAGGCCC GCTACCACGA CCGGGCCCGG
    GACCCCAGCA GCTCCGTGGG CCTGGACACC CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC
    GAGCTGAGCC GGGAGGGGCC TTACGGTCGC GAGTCGGACC ACCACGCCCG GGAGGGCGGC
    CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG GCGGAGCGGG GCAAGGCGGG CGACCCGCAC
    CGGAGGCACG CGCACCGGCA GGCGGGCAGC CGCGAGAGCC GCAGCGGGTC CCCGCGCACC
    GGGGCCGACG GAGAGCCCCG CCGGCACCGG GCGCACCGCA GGCCGGGCGA CGAGGCCCCG
    GACGACAAGG CCGATCGGAG GTCCCGGCAC CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC
    GAGGGCGAGA GCGAGGGCCC AGACGGCGGC GGCGAGCGGA GGCGGCGGCA CCGGCATGGC
    CCTCCCCCAG CGTACGACGC GGACATGCGC AGGGAGGACA AGGAGCGCAG GCACCGCAGG
    AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC GTGTCAGGCC CCAACCTGTC GACCACACGG
    CCAATCCAGC AGGACCTGGG CCGCCAAGAC CCCCCGCTGG CCGAGGATAT CGACAACATG
    AAGAACAACA AACTGGCCAC TGCGGAGTCA GCCAGTCCCC ACGACAGCCT CAGCCACGCC
    GGCCTGCCCC AGAGCCCAGC CAAGATGGGG AACAGCATGG ACCCTGGCCC CACACCGGCC
    CCCACCACCA CGGCCACCAA CCCTCAGAAT GCCGTCAGTC GCCGGACAGC CAACAACCCG
    GGGAACCCGT CCAACCCCGG CCCCCCCAAG ACCCCTGAGA ACAGCCTTAT CGTCACCAAC
    CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG ACTGCCAGGA AACCTGACCA CACCACGGTG
    GACATCCCCC CAGCCTGCCC ACCCCCACTC AACCACACCA TCATCCAAGT GAACAAAAAC
    GCCAACCCAG ACCCACTGCC AAAAAAGGAG GAAGAGAAGA AAGAGGAGGA GGAAGACGAC
    CCCGGGGAAG ACGGCCCCAA GCCCATGCCC CCCTACAGCT CCATGTTTAT CCTCTCTACG
    ACCAACCCCC TTCGCCGCCT GTGCCATTAC ATCCTGAACC TGCGCTACTT TGAGATGTGC
    ATTCTCATGG TCATCGCCAT GAGCAGCATC GCCCTGGCGG CCGAGGATCC TGTTCAGCCC
    AACGCACCAC GAAACAATGT GCTGCGATAT TTTGACTACG TTTTTACAGG CGTCTTTACC
    TTTGAGATGG TGATCAAGAT GATCGACCTG GGGCTCGTCC TGCACCAGGG TGCCTACTTC
    CGTGACCTCT GGAACATTCT CGACTTCATA GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC
    TTCACGAGCA GTACACGTGG CAATAGCAAA GGAAAGGACA TCAACACGAT TAAATCCCTC
    CGAGTCCTCC GGGTGCTACG ACCTCTTAAA ACCATCAAGC GGTTGCCAAA GCTCAAGGCC
    GTGTTTGACT GCGTGGTGAA CTCTCTCAAG AATGTCTTCA ACATCCTCAT CGTCTACATG
    CTGTTCATGT TCATCTTCGC CGTGGTGGCC GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC
    TGCACAGATG AGTCCAAGGA GTTTGAGAAA GATTGCCGCG GCAAGTACCT CCTCTATGAG
    AAGAATGAGG TAAAGGCGAG GGATCGAGAG TGGAAGAAGT ATGAGTTCCA TTATGACAAT
    GTGCTGTGGG CGCTGCTGAC CCTCTTCACC GTGTCCACGG GAGAAGGCTG GCCACAGGTC
    CTCAAGCACT CCGTGGACGC CACCTTCGAG AACCAGGGCC CCAGCCCCGG GTACCGCATG
    GAGATGTCCA TCTTCTACGT CGTCTACTTT GTGGTGTTCC CCTTCTTCTT TGTCAACATC
    TTTGTGGCCT TGATCATCAT CACCTTCCAG GAGCAGGGGG ACAAGATGAT GGAGGAGTAC
    AGCCTGGAGA AAAATGAGAG GGCGTGCATC GACTTCGCCA TCAGCGCCAA ACCCCTGACC
    CGGCACATGC CGCAGAACAA GCAGAGTTTC CAGTACCGCA TGTGGCAGTT CGTGGTGTCC
    CCGCCTTTCG AGTACACCAT CATGGCCATG ATCGCCCTCA ACACCATCGT GCTCATGATG
    AAGTTCTACG GGGCCTCTGT TGCTTATGAG AATGCTCTGA GAGTATTCAA CATCGTCTTC
    ACCTCCCTCT TCTCACTGGA ATGTGTGCTG AAAGTCATGG CTTTTGGGAT TCTGAATTAT
    TTTCGCGATG CCTGGAACAT CTTCGACTTT GTGACTGTTC TGGGCAGCAT CACCGACATC
    CTCGTGACCG AGTTTGGGAA TAACTTCATC AACTTGAGCT TCCTCCGCCT CTTCCGAGCT
    GCCCGGCTCA TCAAACTCCT CCGTCAGGGT TACACCATCC GAATTCTTCT CTGGACCTTT
    GTGCAGTCCT TCAAGGCCCT GCCCTATGTC TGTCTACTGA TTGCCATGCT TTTTTTCATC
    TACGCCATCA TTGGGATGCA GGTGTTTGGC AACATTGGCA TCGATGTGGA GGACGAGGAC
    AGTGATGAAG ATGAATTCCA AATCACTGAG CACAATAACT TCCGCACCTT CTTCCAGGCC
    CTCATGCTTC TCTTCCGGAG TGCCACTGGG GAGGCTTGGC ACAACATCAT GCTTTCCTGC
    CTCAGCGGGA AACCCTGTGA TAAGAACTCT GGCATCCTGA CTCACGAATG CGGCAATGAA
    TTTGCTTATT TTTACTTCGT TTCCTTCATC TTCCTCTGCT CGTTTCTGAT GCTGAATCTT
    TTTGTTGCCG TCATCATGGA CAACTTTGAG TACCTCACCC GAGATTCCTC CATCCTGGGT
    CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCCGAGT ATGACCCTGC AGCGTGCGGT
    CGGATTCATT ATAAGGATAT GTACAGTTTA TTGCGCGTAA TATCTCCCCC TCTCGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCC TGCGGATGGA TCTGCCCGTC
    GCTGATGACA ACACCGTCCA CTTCAACTCT ACCCTCATGG CTCTGATCCG CACGGCCCTG
    GACATCAAAA TTGCCAAAGG TGGAGCTGAC AAACAACAAA TGGATGCTGA GTTGCGGAAG
    GAGATGATGG CCATCTGGCC CAATCTGTCC CAGAAGACGC TGGACCTGCT GGTCACCCCC
    CACAAGTCCA CGGACCTGAC AGTGGGGAAG ATCTATGCGG CCATGATGAT CATGGAGTAT
    TACCGGCAGA GCAAGGCCAA GAAGCTTCAG GCCATGCGCG AGGAACAGAA CCGGACACCT
    CTCATGTTCC AGCGCATGGA GCCCCCATCG CCAACACAGG AGGGGGGGCC TGGCCAGAAC
    GCCCTCCCCT CCACCCAGCT GGACCCAGGA GGAGGCTTGA TGGCTCACGA AAGTGGCATG
    AAAGAGAGCC CATCCTGGGT GACCCAGCGG GCCCAGGAGA TGTTCCAGAA GACGGGCACA
    TGGAGCCCAG AGCGGGGCCC CCCCACTGAT ATGCCTAATA GCCAACCCAA CTCTCAGTCT
    GTGGAGATGC GAGAGATGGG CAGAGACGGC TACTCTGACA GTGAGCACTA CTTCCCCGTT
    GAAGGCCAGG CCCGGGCCGC CTCCATGCCC CGCCTGCCTG CCGAGAACCA GAGGAGAAGG
    GGCCGGCCAC GTGGGAATAA CCTCAGTACC ATCTCAGACA CCAGCCCCAT GAAGCGCTCG
    GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC CTGGACGACT ACTCCCTGGA GAGGGTGCCG
    CCCGAGGAGA ACCAGCGGCA CCACCAGAGG CGCAGAGACC GTGGCCACCG CACCTCCGAG
    CGCTCCTTGG GCCGCTACAC GGACGTGGAC ACAGGCTTGG GGACAGACTT GAGCATGACC
    ACCCAGTCTG GGGACCTGCC ATCAAAGGAA AGAGACCAGG AGCGGGGCCG GCCCAAGGAC
    CGGAAGCATC GGCAACACCA CCACCACCAC CACCACCACC ATCCCCCGCC CCCTGACAAG
    GAGCGCTACA CCCAGGAGCG GCCTGAGCAC GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG
    TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC ATGGCCCACC GTCAGGGCAG TAGTTCCGTA
    AGTGGAAGCC CAGCCCCCTC AACATCTGGT ACCAGCACTC CGCGGCGGGG ACGCCGCCAG
    CTCCCCCAGA CCCCCTCCAC TCCCCGGCCG CACGTGTCCT ATTCCCCCGT GATCCGTAAG
    GCCGGCGGCT CGGGTCCCCA GCAGCAGCAG CAGCCCGCGG CGCGGCCGGG CCGAACGGCC
    CCCAGCGGCC CGCGGAGGTA CCCGGGCCCC GTGGCCGAGC CCCTGGCCGG GGAGCGGCCT
    CCCACGGGAG GCCACGGAAG CAGCCGCTCG CCCGCGATGG AGCGGCGGAT CCCGGGCCCG
    GCCCGGAGCG AGTCCCCCAG GGCGCCGTGT CGCCACGGCG GGGCGCGATG GCCGGCGTCC
    GGCGCGCACG CGGCCGAGGG CCCCCCGGGC CCCCGGCACC ACCACGGTTA CTACCGGGGC
    TCCGACTACG ACGAGCCAGA CGGCCCGGGC GGCGGCGGCG GGGAGGAGGA GGAGCCCCGG
    GCCGCCGCCT ACGACGCGCC GCCCCCCGCG CGCCACGGGT GCTCGCCCAG GACTCCCCGG
    GCCGCGGGCC CGGCCTGCGC CTCGCCTTCC AGGCACGGCC GGCGACTCCC GAACGGCTAC
    TACCCGGCCC ACGGACTGGC CAGGCCCCGC GGGCCGGGCT CCCGGAAAGG CCTGCACGAA
    GCCTACAGCG AGACCGACGA CGATGACTGG TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235780
    Clone ID Related Accession (Same CDS sequence) XM_024993859.1
    Accession Version XM_024993859.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA
    TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC
    CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG
    CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC
    ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA
    AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC
    CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC
    GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC
    ATGGCCCACC GTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849627.1
    CDS230..7027
    Translation

    Target ORF information:

    RefSeq Version XM_024993859.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGGAGCT
    CTGCGGAGAG CCACTATAAA GAAGAGCAAG ACAGACCTGC TCAACCCCGA GGAGGCTGAG
    GATCAGCTGG CCGACATCGC CTCTGTGGGG TCTCCCTTCG CCCGAGCCAG CATTAAAAGT
    GCCAAGCTGG AGAACTCAAC TTTTTTTCAC AAAAAGGAGA GGAGGATGCG TTTCTACATC
    CGCCGCATGG TCAAAACTCA GGCCTTCTAC TGGACTGTGC TCAGTCTGGT AGCCCTCAAC
    ACGCTGTGTG TCGCTATCGT TCACTACAAC CAGCCTGAGT GGCTCTCCGA CTTCCTCTAC
    TATGCAGAAT TCATTTTCTT AGGACTCTTT ATGTCCGAAA TGTTTATAAA AATGTACGGG
    CTTGGGACGC GGCCTTACTT CCACTCTTCC TTCAACTGTT TTGATTGTGG GGTCATCATC
    GGGAGTATCT TTGAGGTCAT CTGGGCTGTC ATAAAACCCG GCACATCCTT TGGAATCAGT
    GTGTTACGAG CCCTCAGGTT ATTGCGTATT TTCAAAGTCA CAAAGTACTG GGCATCTCTC
    AGGAACCTGG TCGTCTCTCT TCTCAACTCC ATGAAATCCA TCATCAGCCT GTTGTTCCTC
    CTTTTCCTAT TCATCGTCGT CTTTGCCCTT CTGGGAATGC AACTCTTTGG CGGCCAGTTT
    AATTTCGATG AAGGGACTCC TCCTACCAAC TTCGACACTT TTCCAGCAGC AATAATGACA
    GTTTTTCAGA TCCTGACGGG CGAAGACTGG AACGAGGTCA TGTATGACGG CATCAAGTCT
    CAGGGGGGCG TGCAAGGCGG CATGGTGTTC TCCATCTACT TCATCGTGCT GACGCTCTTC
    GGGAACTACA CCCTCCTGAA CGTGTTCTTG GCCATCGCGG TGGATAATCT GGCTAACGCC
    CAGGAGCTCA CCAAGGATGA ACAAGAAGAA GAAGAGGCTG CCAACCAGAA ACTCGCCCTC
    CAGAAAGCCA AAGAGGTGGC AGAAGTGAGT CCTCTGTCTG CAGCCAACAT GTCCATAGCT
    GTGAAGGAGC AACAGAAGAA CCAGAAGCCC GCCAAGTCGG TGTGGGAGCA GCGGACAAGC
    GAGATGAGGA AGCAGAATCT TCTGGCCAGT CGGGAGGCAC TCTACAACGA GATGGACCCA
    GACGAGCGCT GGAAGGCCTC CTACGCTCGC CACCTGCGGC CCGACATGAA GACCCACCTG
    GACCGGCCGC TGGTGGTCGA CCCCCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGG
    GCGGCCGAGC CCACGGTGGA CCAGCGCCTC GGGCAGCAGC GAGCCGAGGA CTTCCTCAGG
    AAACAGGCCC GCTACCACGA CCGGGCCCGG GACCCCAGCA GCTCCGTGGG CCTGGACACC
    CGGAGGCCCT GGGCGGGCAG CCAGGAAGCC GAGCTGAGCC GGGAGGGGCC TTACGGTCGC
    GAGTCGGACC ACCACGCCCG GGAGGGCGGC CTGGAGCAGC CGGGGTTCTG GGAGGGCGAG
    GCGGAGCGGG GCAAGGCGGG CGACCCGCAC CGGAGGCACG CGCACCGGCA GGCGGGCAGC
    CGCGAGAGCC GCAGCGGGTC CCCGCGCACC GGGGCCGACG GAGAGCCCCG CCGGCACCGG
    GCGCACCGCA GGCCGGGCGA CGAGGCCCCG GACGACAAGG CCGATCGGAG GTCCCGGCAC
    CGCGAGGGCA GCCGGCCGGC CCGGGGCGGC GAGGGCGAGA GCGAGGGCCC AGACGGCGGC
    GGCGAGCGGA GGCGGCGGCA CCGGCATGGC CCTCCCCCAG CGTACGACGC GGACATGCGC
    AGGGAGGACA AGGAGCGCAG GCACCGCAGG AGGAAAGAGA ACCAGGGCTC CGGGGTCCCC
    GTGTCAGGCC CCAACCTGTC GACCACACGG CCAATCCAGC AGGACCTGGG CCGCCAAGAC
    CCCCCGCTGG CCGAGGATAT CGACAACATG AAGAACAACA AACTGGCCAC TGCGGAGTCA
    GCCAGTCCCC ACGACAGCCT CAGCCACGCC GGCCTGCCCC AGAGCCCAGC CAAGATGGGG
    AACAGCATGG ACCCTGGCCC CACACCGGCC CCCACCACCA CGGCCACCAA CCCTCAGAAT
    GCCGTCAGTC GCCGGACAGC CAACAACCCG GGGAACCCGT CCAACCCCGG CCCCCCCAAG
    ACCCCTGAGA ACAGCCTTAT CGTCACCAAC CCCAGCAGCA CCCAGGCCAA CTCAGCTAAG
    ACTGCCAGGA AACCTGACCA CACCACGGTG GACATCCCCC CAGCCTGCCC ACCCCCACTC
    AACCACACCA TCATCCAAGT GAACAAAAAC GCCAACCCAG ACCCACTGCC AAAAAAGGAG
    GAAGAGAAGA AAGAGGAGGA GGAAGACGAC CCCGGGGAAG ACGGCCCCAA GCCCATGCCC
    CCCTACAGCT CCATGTTTAT CCTCTCTACG ACCAACCCCC TTCGCCGCCT GTGCCATTAC
    ATCCTGAACC TGCGCTACTT TGAGATGTGC ATTCTCATGG TCATCGCCAT GAGCAGCATC
    GCCCTGGCGG CCGAGGATCC TGTTCAGCCC AACGCACCAC GAAACAATGT GCTGCGATAT
    TTTGACTACG TTTTTACAGG CGTCTTTACC TTTGAGATGG TGATCAAGAT GATCGACCTG
    GGGCTCGTCC TGCACCAGGG TGCCTACTTC CGTGACCTCT GGAACATTCT CGACTTCATA
    GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC TTCACGAGCA GTACACGTGG CAATAGCAAA
    GGAAAGGACA TCAACACGAT TAAATCCCTC CGAGTCCTCC GGGTGCTACG ACCTCTTAAA
    ACCATCAAGC GGTTGCCAAA GCTCAAGGCC GTGTTTGACT GCGTGGTGAA CTCTCTCAAG
    AATGTCTTCA ACATCCTCAT CGTCTACATG CTGTTCATGT TCATCTTCGC CGTGGTGGCC
    GTGCAGCTCT TCAAGGGCAA GTTTTTCCAC TGCACAGATG AGTCCAAGGA GTTTGAGAAA
    GATTGCCGCG GCAAGTACCT CCTCTATGAG AAGAATGAGG TAAAGGCGAG GGATCGAGAG
    TGGAAGAAGT ATGAGTTCCA TTATGACAAT GTGCTGTGGG CGCTGCTGAC CCTCTTCACC
    GTGTCCACGG GAGAAGGCTG GCCACAGGTC CTCAAGCACT CCGTGGACGC CACCTTCGAG
    AACCAGGGCC CCAGCCCCGG GTACCGCATG GAGATGTCCA TCTTCTACGT CGTCTACTTT
    GTGGTGTTCC CCTTCTTCTT TGTCAACATC TTTGTGGCCT TGATCATCAT CACCTTCCAG
    GAGCAGGGGG ACAAGATGAT GGAGGAGTAC AGCCTGGAGA AAAATGAGAG GGCGTGCATC
    GACTTCGCCA TCAGCGCCAA ACCCCTGACC CGGCACATGC CGCAGAACAA GCAGAGTTTC
    CAGTACCGCA TGTGGCAGTT CGTGGTGTCC CCGCCTTTCG AGTACACCAT CATGGCCATG
    ATCGCCCTCA ACACCATCGT GCTCATGATG AAGTTCTACG GGGCCTCTGT TGCTTATGAG
    AATGCTCTGA GAGTATTCAA CATCGTCTTC ACCTCCCTCT TCTCACTGGA ATGTGTGCTG
    AAAGTCATGG CTTTTGGGAT TCTGAATTAT TTTCGCGATG CCTGGAACAT CTTCGACTTT
    GTGACTGTTC TGGGCAGCAT CACCGACATC CTCGTGACCG AGTTTGGGAA TAACTTCATC
    AACTTGAGCT TCCTCCGCCT CTTCCGAGCT GCCCGGCTCA TCAAACTCCT CCGTCAGGGT
    TACACCATCC GAATTCTTCT CTGGACCTTT GTGCAGTCCT TCAAGGCCCT GCCCTATGTC
    TGTCTACTGA TTGCCATGCT TTTTTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGC
    AACATTGGCA TCGATGTGGA GGACGAGGAC AGTGATGAAG ATGAATTCCA AATCACTGAG
    CACAATAACT TCCGCACCTT CTTCCAGGCC CTCATGCTTC TCTTCCGGAG TGCCACTGGG
    GAGGCTTGGC ACAACATCAT GCTTTCCTGC CTCAGCGGGA AACCCTGTGA TAAGAACTCT
    GGCATCCTGA CTCACGAATG CGGCAATGAA TTTGCTTATT TTTACTTCGT TTCCTTCATC
    TTCCTCTGCT CGTTTCTGAT GCTGAATCTT TTTGTTGCCG TCATCATGGA CAACTTTGAG
    TACCTCACCC GAGATTCCTC CATCCTGGGT CCCCACCACC TGGATGAGTA CGTGCGTGTC
    TGGGCCGAGT ATGACCCTGC AGCGTGCGGT CGGATTCATT ATAAGGATAT GTACAGTTTA
    TTGCGCGTAA TATCTCCCCC TCTCGGCTTA GGCAAGAAAT GTCCTCATAG GGTTGCTTGC
    AAGAGGCTCC TGCGGATGGA TCTGCCCGTC GCTGATGACA ACACCGTCCA CTTCAACTCT
    ACCCTCATGG CTCTGATCCG CACGGCCCTG GACATCAAAA TTGCCAAAGG TGGAGCTGAC
    AAACAACAAA TGGATGCTGA GTTGCGGAAG GAGATGATGG CCATCTGGCC CAATCTGTCC
    CAGAAGACGC TGGACCTGCT GGTCACCCCC CACAAGTCCA CGGACCTGAC AGTGGGGAAG
    ATCTATGCGG CCATGATGAT CATGGAGTAT TACCGGCAGA GCAAGGCCAA GAAGCTTCAG
    GCCATGCGCG AGGAACAGAA CCGGACACCT CTCATGTTCC AGCGCATGGA GCCCCCATCG
    CCAACACAGG AGGGGGGGCC TGGCCAGAAC GCCCTCCCCT CCACCCAGCT GGACCCAGGA
    GGAGGCTTGA TGGCTCACGA AAGTGGCATG AAAGAGAGCC CATCCTGGGT GACCCAGCGG
    GCCCAGGAGA TGTTCCAGAA GACGGGCACA TGGAGCCCAG AGCGGGGCCC CCCCACTGAT
    ATGCCTAATA GCCAACCCAA CTCTCAGTCT GTGGAGATGC GAGAGATGGG CAGAGACGGC
    TACTCTGACA GTGAGCACTA CTTCCCCGTT GAAGGCCAGG CCCGGGCCGC CTCCATGCCC
    CGCCTGCCTG CCGAGAACCA GAGGAGAAGG GGCCGGCCAC GTGGGAATAA CCTCAGTACC
    ATCTCAGACA CCAGCCCCAT GAAGCGCTCG GCCTCCGTGC TGGGCCCCAA GGCCCGGCGC
    CTGGACGACT ACTCCCTGGA GAGGGTGCCG CCCGAGGAGA ACCAGCGGCA CCACCAGAGG
    CGCAGAGACC GTGGCCACCG CACCTCCGAG CGCTCCTTGG GCCGCTACAC GGACGTGGAC
    ACAGGCTTGG GGACAGACTT GAGCATGACC ACCCAGTCTG GGGACCTGCC ATCAAAGGAA
    AGAGACCAGG AGCGGGGCCG GCCCAAGGAC CGGAAGCATC GGCAACACCA CCACCACCAC
    CACCACCACC ATCCCCCGCC CCCTGACAAG GAGCGCTACA CCCAGGAGCG GCCTGAGCAC
    GGCCGGCCCC GGGCCCGTGA CCAGCGCTGG TCCCGCTCAC CCAGTGAGGG CCGGGAGCAC
    ATGGCCCACC GTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01216
    Clone ID Related Accession (Same CDS sequence) NM_001075129.1
    Accession Version NM_001075129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ207750.1. On Oct 28, 2006 this sequence version replaced XM_613176.2. ##Evidence-Data-START## Transcript exon combination :: DQ207750.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145431, SAMN03145437 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGTTGGA
    GCTCTGCGGA GAGCCACTAT AAAGAAGAGC AAGACAGACC TGCTCAACCC CGAGGAGGCT
    GAGGATCAGC TGGCCGACAT CGCCTCTGTG GGGTCTCCCT TCGCCCGAGC CAGCATTAAA
    AGTGCCAAGC TGGAGAACTC AACTTTTTTT CACAAAAAGG AGAGGAGGAT GCGTTTCTAC
    ATCCGCCGCA TGGTCAAAAC TCAGGCCTTC TACTGGACTG TGCTCAGTCT GGTAGCCCTC
    AACACGCTGT GTGTCGCTAT CGTTCACTAC AACCAGCCTG AGTGGCTCTC CGACTTCCTC
    TACTATGCAG AATTCATTTT CTTAGGACTC TTTATGTCCG AAATGTTTAT AAAAATGTAC
    GGGCTTGGGA CGCGGCCTTA CTTCCACTCT TCCTTCAACT GTTTTGATTG TGGGGTCATC
    ATCGGGAGTA TCTTTGAGGT CATCTGGGCT GTCATAAAAC CCGGCACATC CTTTGGAATC
    AGTGTGTTAC GAGCCCTCAG GTTATTGCGT ATTTTCAAAG TCACAAAGTA CTGGGCATCT
    CTCAGGAACC TGGTCGTCTC TCTTCTCAAC TCCATGAAAT CCATCATCAG CCTGTTGTTC
    CTCCTTTTCC TATTCATCGT CGTCTTTGCC CTTCTGGGAA TGCAACTCTT TGGCGGCCAG
    TTTAATTTCG ATGAAGGGAC TCCTCCTACC AACTTCGACA CTTTTCCAGC AGCAATAATG
    ACAGTTTTTC AGATCCTGAC GGGCGAAGAC TGGAACGAGG TCATGTATGA CGGCATCAAG
    TCTCAGGGGG GCGTGCAAGG CGGCATGGTG TTCTCCATCT ACTTCATCGT GCTGACGCTC
    TTCGGGAACT ACACCCTCCT GAACGTGTTC TTGGCCATCG CGGTGGATAA TCTGGCTAAC
    GCCCAGGAGC TCACCAAGGA TGAACAAGAA GAAGAAGAGG CAGCCAACCA GAAACTTGCC
    CTACAGAAAG CCAAGGAGGT GGCAGAAGTG AGTCCTCTGT CTGCAGCCAA CATGTCCATA
    GCTGTGAAGG AGCAACAGAA GAACCAGAAG CCCGCCAAGT CGGTGTGGGA GCAGCGGACA
    AGCGAGATGA GGAAGCAGAA TCTTCTGGCC AGTCGGGAGG CACTCTACAA CGAGATGGAC
    CCAGACGAGC GCTGGAAGGC CTCCTACGCT CGCCACCTGC GGCCCGACAT GAAGACCCAC
    CTGGACCGGC CGCTGGTGGT CGACCCCCAG GAGAACCGTA ACAACAACAC CAACAAGAGC
    CGGGCGGCCG AGCCCACGGT GGACCAGCGC CTCGGGCAGC AGCGAGCCGA GGACTTCCTC
    AGGAAACAGG CCCGCTACCA CGACCGGGCC CGGGACCCCA GCAGCTCCGT GGGCCTGGAC
    ACCCGGAGGC CCTGGGCGGG CAGCCAGGAA GCCGAGCTGA GCCGGGAGGG GCCTTACGGT
    CGCGAGTCGG ACCACCACGC CCGGGAGGGC GGCCTGGAGC AGCCGGGGTT CTGGGAGGGC
    GAGGCGGAGC GGGGCAAGGC GGGCGACCCG CACCGGAGGC ACGCGCACCG GCAGGCGGGC
    AGCCGCGAGA GCCGCAGCGG GTCCCCGCGC ACCGGGGCCG ACGGAGAGCC CCGCCGGCAC
    CGGGCGCACC GCAGGCCGGG CGACGAGGCC CCGGACGACA AGGCCGATCG GAGGTCCCGG
    CACCGCGAGG GCAGCCGGCC GGCCCGGGGC GGCGAGGGCG AGAGCGAGGG CCCAGACGGC
    GGCGGCGAGC GGAGGCGGCG GCACCGGCAT GGCCCTCCCC CAGCGTACGA CGCGGACATG
    CGCAGGGAGG ACAAGGAGCG CAGGCACCGC AGGAGGAAAG AGAACCAGGG CTCCGGGGTC
    CCCGTGTCAG GCCCCAACCT GTCGACCACA CGGCCAATCC AGCAGGACCT GGGCCGCCAA
    GACCCCCCGC TGGCCGAGGA TATCGACAAC ATGAAGAACA ACAAACTGGC CACTGCGGAG
    TCAGCCAGTC CCCACGACAG CCTCAGCCAC GCCGGCCTGC CCCAGAGCCC AGCCAAGATG
    GGGAACAGCA TGGACCCTGG CCCCACACCG GCCCCCACCA CCACGGCCAC CAACCCTCAG
    AATGCCGTCA GTCGCCGGAC AGCCAACAAC CCGGGGAACC CGTCCAACCC CGGCCCCCCC
    AAGACCCCTG AGAACAGCCT TATCGTCACC AACCCCAGCA GCACCCAGGC CAACTCAGCT
    AAGACTGCCA GGAAACCTGA CCACACCACG GTGGACATCC CCCCAGCCTG CCCCCCCCCA
    CTCAACCACA CCATCATCCA AGTGAACAAA AACGCCAACC CAGACCCACT GCCAAAAAAG
    GAGGAAGAGA AGAAAGAGGA GGAGGAAGAC GACCCCGGGG AAGACGGCCC CAAGCCCATG
    CCCCCCTACA GCTCCATGTT TATCCTCTCT ACGACCAACC CCCTTCGCCG CCTGTGCCAT
    TACATCCTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCA TGGTCATCGC CATGAGCAGC
    ATCGCCCTGG CGGCCGAGGA TCCTGTTCAG CCCAACGCAC CACGAAACAA TGTGCTGCGA
    TATTTTGACT ACGTTTTTAC AGGCGTCTTT ACCTTTGAGA TGGTGATCAA GATGATCGAC
    CTGGGGCTCG TCCTGCACCA GGGTGCCTAC TTCCGTGACC TCTGGAACAT TCTCGACTTC
    ATAGTGGTCA GTGGGGCCCT GGTGGCCTTT GCCTTCACTG GCAATAGCAA AGGAAAGGAC
    ATCAACACGA TTAAATCCCT CCGAGTCCTC CGGGTGCTAC GACCTCTTAA AACCATCAAG
    CGGTTGCCAA AGCTCAAGGC CGTGTTTGAC TGCGTGGTGA ACTCGCTCAA GAACGTCTTC
    AACATCCTCA TCGTCTACAT GCTGTTCATG TTCATCTTCG CCGTGGTGGC CGTGCAGCTC
    TTCAAGGGCA AGTTTTTCCA CTGCACGGAT GAGTCCAAGG AGTTTGAGAA AGATTGTCGC
    GGCAAGTACC TCCTCTATGA GAAGAATGAG GTAAAGGCGA GGGATCGAGA GTGGAAGAAG
    TATGAGTTCC ATTATGACAA TGTGCTGTGG GCGCTGCTGA CCCTCTTCAC CGTGTCCACG
    GGAGAAGGCT GGCCACAGGT CCTCAAGCAC TCGGTGGACG CCACCTTTGA GAACCAGGGC
    CCCAGCCCCG GGTACCGCAT GGAGATGTCC ATCTTCTACG TCGTCTACTT TGTGGTGTTC
    CCCTTCTTCT TTGTCAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG
    GACAAGATGA TGGAGGAGTA CAGCCTGGAG AAAAATGAGA GGGCGTGCAT CGACTTCGCC
    ATCAGCGCCA AACCCCTGAC CCGGCACATG CCGCAGAACA AGCAGAGTTT CCAGTACCGC
    ATGTGGCAGT TCGTGGTGTC CCCGCCTTTC GAGTACACCA TCATGGCCAT GATCGCCCTC
    AACACCATCG TGCTCATGAT GAAGTTCTAC GGGGCCTCTG TTGCTTATGA GAATGCTCTG
    AGAGTATTCA ACATCGTCTT CACCTCCCTC TTCTCACTGG AATGTGTGCT GAAAGTCATG
    GCTTTTGGGA TTCTGAATTA TTTTCGCGAT GCCTGGAACA TCTTCGACTT TGTGACTGTT
    CTGGGCAGCA TCACCGACAT CCTCGTGACC GAGTTTGGGA ATAACTTCAT CAACTTGAGC
    TTCCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTCC TCCGTCAGGG TTACACCATC
    CGAATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCCTATGT CTGTCTACTG
    ATTGCCATGC TTTTTTTCAT CTACGCCATC ATTGGGATGC AGGTGTTTGG CAACATTGGC
    ATCGATGTGG AGGACGAGGA CAGTGATGAA GATGAATTCC AAATCACTGA GCACAATAAC
    TTCCGCACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACTGG GGAGGCTTGG
    CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCCTGTG ATAAGAACTC TGGCATCCTG
    ACTCACGAAT GCGGCAATGA ATTTGCTTAT TTTTACTTCG TTTCCTTCAT CTTCCTCTGC
    TCGTTTCTGA TGCTGAATCT TTTTGTTGCC GTCATCATGG ACAACTTTGA GTACCTCACC
    CGAGATTCCT CCATCCTGGG TCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG
    TATGACCCTG CAGCGTGCGG TCGGATTCAT TATAAGGATA TGTACAGTTT ATTGCGTGTA
    ATATCTCCCC CTCTCGGCTT AGGCAAGAAA TGTCCTCATA GGGTTGCTTG CAAGAGGCTC
    CTGCGGATGG ATCTGCCCGT CGCTGATGAC AACACCGTCC ACTTCAACTC TACCCTCATG
    GCTCTGATCC GCACGGCCCT GGACATCAAA ATTGCCAAAG GTGGAGCTGA CAAACAACAA
    ATGGATGCTG AGTTGCGGAA GGAGATGATG GCCATCTGGC CCAATCTGTC CCAGAAGACG
    CTGGACCTGC TGGTCACCCC CCACAAGTCC ACGGACCTGA CAGTGGGGAA GATCTATGCG
    GCCATGATGA TCATGGAGTA TTACCGGCAG AGCAAGGCCA AGAAGCTTCA GGCCATGCGC
    GAGGAACAGA ACCGGACACC TCTCATGTTC CAGCGCATGG AGCCCCCATC GCCAACACAG
    GAGGGGGGGC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGGCTTG
    ATGGCTCACG AAAGTGGCAT GAAAGAGAGC CCATCCTGGG TGACCCAGCG GGCCCAGGAG
    ATGTTCCAGA AGACGGGCAC ATGGAGCCCA GAGCGGGGCC CCCCCACTGA TATGCCTAAT
    AGCCAACCCA ACTCTCAGTC TGTGGAGATG CGAGAGATGG GCAGAGACGG CTACTCTGAC
    AGTGAGCACT ACTTCCCCGT TGAAGGCCAG GCCCGGGCCG CCTCCATGCC CCGCCTGCCT
    GCCGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC
    ACCAGCCCCA TGAAGCGCTC GGCCTCCGTG CTGGGCCCCA AGGCCCGGCG CCTGGACGAC
    TACTCCCTGG AGAGGGTGCC GCCCGAGGAG AACCAGCGGC ACCACCAGAG GCGCAGAGAC
    CGTGGCCACC GCACCTCCGA GCGCTCCTTG GGCCGCTACA CGGACGTGGA CACAGGCTTG
    GGGACAGACC TGAGCATGAC CACCCAATCT GGGGACCTGC CATCAAAGGA AAGAGACCAG
    GAGCGGGGCC GGCCCAAGGA CCGGAAGCAT CGGCAACACC ACCACCACCA CCACCACCAC
    CACCATCCCC CGCCCCCTGA CAAGGAGCGC TACACCCAGG AGCGGCCTGA GCACGGCCGG
    CCCCGGGCCC GTGACCAGCG CTGGTCCCGC TCACCCAGTG AGGGCCGGGA GCACATGGCC
    CACCGTCAGG GCAGTAGTTC CGTAAGTGGA AGCCCAGCCC CCTCAACATC TGGTACCAGC
    ACTCCGCGGC GGGGACGCCG CCAGCTCCCC CAGACCCCCT CCACTCCCCG GCCGCACGTG
    TCCTATTCCC CCGTGATCCG TAAGGCCGGC GGCTCGGGTC CCCAGCAGCA GCAGCAGCAG
    CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG
    GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC
    GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC
    CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC
    CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGGCCGATGG CCCGGGCGGC
    GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC
    CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG
    CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG
    CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA GCGACGACGA TTGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001068597.1
    CDS32..7531
    Translation

    Target ORF information:

    RefSeq Version NM_001075129.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 A (CACNA1A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001075129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCCCGCT TCGGAGACGA GATGCCGGCC CGCTACGGGG GAGGAGGCTC CGGGGCAGCC 
    GCCGGGGTGG TCGTGGGCGC CGGAGGCGGG CGAGGAGCCG GGGGCAGCCG GCAGGGCGGG
    CAGCCCGGGG CGCAAAGGAT GTACAAGCAG TCAATGGCGC AGAGAGCGCG GACCATGGCC
    CTCTACAACC CCATCCCCGT CCGACAGAAC TGCCTCACGG TCAACCGGTC TCTATTCCTC
    TTCAGCGAGG ACAACGTGGT GAGAAAATAC GCCAAAAAGA TCACCGAATG GCCTCCCTTT
    GAATATATGA TTTTAGCCAC CATCATAGCA AACTGCATCG TCCTCGCCCT GGAGCAACAC
    CTACCTGATG ATGACAAGAC TCCGATGTCT GAACGACTGG ATGACACAGA ACCATACTTC
    ATTGGAATTT TTTGTTTCGA GGCTGGAATT AAAATCATCG CCCTTGGATT TGCCTTCCAT
    AAAGGCTCTT ACTTGAGGAA CGGCTGGAAT GTGATGGACT TTGTGGTGGT GCTAACAGGC
    ATCTTGGCAA CAGTTGGGAC GGAGTTTGAC CTAAGGACGC TGAGGGCAGT TCGAGTGCTG
    CGGCCGCTCA AGCTGGTGTC TGGAATCCCA AGTTTACAAG TCGTCCTGAA GTCGATCATG
    AAGGCAATGA TCCCCCTGCT GCAGATCGGC CTCCTCCTAT TTTTTGCAAT CCTTATTTTT
    GCAATCATAG GGTTAGAATT TTATATGGGA AAATTTCATA CCACCTGCTT TGAAGAGGGC
    ACAGATGACA TTCAGGGTGA GTCTCCAGCA CCGTGTGGGA CAGAGGAACC TGCCCGCACC
    TGCCCCAATG GGACCAAATG TCAGCCCTAC TGGGAAGGAC CCAACAATGG GATCACTCAG
    TTCGACAACA TCCTGTTTGC AGTGCTGACT GTTTTCCAGT GCATCACCAT GGAAGGGTGG
    ACTGACCTCC TCTACAACAG CAACGATGCC TCAGGGAACA CTTGGAACTG GTTGTACTTC
    ATCCCCCTCA TCATCATCGG CTCCTTTTTT ATGCTGAACC TCGTGCTGGG TGTGCTTTCT
    GGGGAGTTTG CCAAAGAAAG GGAGCGGGTG GAGAACCGGC GGGCATTTCT GAAGCTGAGG
    CGGCAGCAGC AGATTGAACG AGAGCTCAAT GGGTACATGG AGTGGATCTC AAAAGCAGAA
    GAGGTGATCC TCGCCGAAGA TGAAACTGAT GGGGAGCAGA GACATCCCTT TGATGTTGGA
    GCTCTGCGGA GAGCCACTAT AAAGAAGAGC AAGACAGACC TGCTCAACCC CGAGGAGGCT
    GAGGATCAGC TGGCCGACAT CGCCTCTGTG GGGTCTCCCT TCGCCCGAGC CAGCATTAAA
    AGTGCCAAGC TGGAGAACTC AACTTTTTTT CACAAAAAGG AGAGGAGGAT GCGTTTCTAC
    ATCCGCCGCA TGGTCAAAAC TCAGGCCTTC TACTGGACTG TGCTCAGTCT GGTAGCCCTC
    AACACGCTGT GTGTCGCTAT CGTTCACTAC AACCAGCCTG AGTGGCTCTC CGACTTCCTC
    TACTATGCAG AATTCATTTT CTTAGGACTC TTTATGTCCG AAATGTTTAT AAAAATGTAC
    GGGCTTGGGA CGCGGCCTTA CTTCCACTCT TCCTTCAACT GTTTTGATTG TGGGGTCATC
    ATCGGGAGTA TCTTTGAGGT CATCTGGGCT GTCATAAAAC CCGGCACATC CTTTGGAATC
    AGTGTGTTAC GAGCCCTCAG GTTATTGCGT ATTTTCAAAG TCACAAAGTA CTGGGCATCT
    CTCAGGAACC TGGTCGTCTC TCTTCTCAAC TCCATGAAAT CCATCATCAG CCTGTTGTTC
    CTCCTTTTCC TATTCATCGT CGTCTTTGCC CTTCTGGGAA TGCAACTCTT TGGCGGCCAG
    TTTAATTTCG ATGAAGGGAC TCCTCCTACC AACTTCGACA CTTTTCCAGC AGCAATAATG
    ACAGTTTTTC AGATCCTGAC GGGCGAAGAC TGGAACGAGG TCATGTATGA CGGCATCAAG
    TCTCAGGGGG GCGTGCAAGG CGGCATGGTG TTCTCCATCT ACTTCATCGT GCTGACGCTC
    TTCGGGAACT ACACCCTCCT GAACGTGTTC TTGGCCATCG CGGTGGATAA TCTGGCTAAC
    GCCCAGGAGC TCACCAAGGA TGAACAAGAA GAAGAAGAGG CAGCCAACCA GAAACTTGCC
    CTACAGAAAG CCAAGGAGGT GGCAGAAGTG AGTCCTCTGT CTGCAGCCAA CATGTCCATA
    GCTGTGAAGG AGCAACAGAA GAACCAGAAG CCCGCCAAGT CGGTGTGGGA GCAGCGGACA
    AGCGAGATGA GGAAGCAGAA TCTTCTGGCC AGTCGGGAGG CACTCTACAA CGAGATGGAC
    CCAGACGAGC GCTGGAAGGC CTCCTACGCT CGCCACCTGC GGCCCGACAT GAAGACCCAC
    CTGGACCGGC CGCTGGTGGT CGACCCCCAG GAGAACCGTA ACAACAACAC CAACAAGAGC
    CGGGCGGCCG AGCCCACGGT GGACCAGCGC CTCGGGCAGC AGCGAGCCGA GGACTTCCTC
    AGGAAACAGG CCCGCTACCA CGACCGGGCC CGGGACCCCA GCAGCTCCGT GGGCCTGGAC
    ACCCGGAGGC CCTGGGCGGG CAGCCAGGAA GCCGAGCTGA GCCGGGAGGG GCCTTACGGT
    CGCGAGTCGG ACCACCACGC CCGGGAGGGC GGCCTGGAGC AGCCGGGGTT CTGGGAGGGC
    GAGGCGGAGC GGGGCAAGGC GGGCGACCCG CACCGGAGGC ACGCGCACCG GCAGGCGGGC
    AGCCGCGAGA GCCGCAGCGG GTCCCCGCGC ACCGGGGCCG ACGGAGAGCC CCGCCGGCAC
    CGGGCGCACC GCAGGCCGGG CGACGAGGCC CCGGACGACA AGGCCGATCG GAGGTCCCGG
    CACCGCGAGG GCAGCCGGCC GGCCCGGGGC GGCGAGGGCG AGAGCGAGGG CCCAGACGGC
    GGCGGCGAGC GGAGGCGGCG GCACCGGCAT GGCCCTCCCC CAGCGTACGA CGCGGACATG
    CGCAGGGAGG ACAAGGAGCG CAGGCACCGC AGGAGGAAAG AGAACCAGGG CTCCGGGGTC
    CCCGTGTCAG GCCCCAACCT GTCGACCACA CGGCCAATCC AGCAGGACCT GGGCCGCCAA
    GACCCCCCGC TGGCCGAGGA TATCGACAAC ATGAAGAACA ACAAACTGGC CACTGCGGAG
    TCAGCCAGTC CCCACGACAG CCTCAGCCAC GCCGGCCTGC CCCAGAGCCC AGCCAAGATG
    GGGAACAGCA TGGACCCTGG CCCCACACCG GCCCCCACCA CCACGGCCAC CAACCCTCAG
    AATGCCGTCA GTCGCCGGAC AGCCAACAAC CCGGGGAACC CGTCCAACCC CGGCCCCCCC
    AAGACCCCTG AGAACAGCCT TATCGTCACC AACCCCAGCA GCACCCAGGC CAACTCAGCT
    AAGACTGCCA GGAAACCTGA CCACACCACG GTGGACATCC CCCCAGCCTG CCCCCCCCCA
    CTCAACCACA CCATCATCCA AGTGAACAAA AACGCCAACC CAGACCCACT GCCAAAAAAG
    GAGGAAGAGA AGAAAGAGGA GGAGGAAGAC GACCCCGGGG AAGACGGCCC CAAGCCCATG
    CCCCCCTACA GCTCCATGTT TATCCTCTCT ACGACCAACC CCCTTCGCCG CCTGTGCCAT
    TACATCCTGA ACCTGCGCTA CTTTGAGATG TGCATTCTCA TGGTCATCGC CATGAGCAGC
    ATCGCCCTGG CGGCCGAGGA TCCTGTTCAG CCCAACGCAC CACGAAACAA TGTGCTGCGA
    TATTTTGACT ACGTTTTTAC AGGCGTCTTT ACCTTTGAGA TGGTGATCAA GATGATCGAC
    CTGGGGCTCG TCCTGCACCA GGGTGCCTAC TTCCGTGACC TCTGGAACAT TCTCGACTTC
    ATAGTGGTCA GTGGGGCCCT GGTGGCCTTT GCCTTCACTG GCAATAGCAA AGGAAAGGAC
    ATCAACACGA TTAAATCCCT CCGAGTCCTC CGGGTGCTAC GACCTCTTAA AACCATCAAG
    CGGTTGCCAA AGCTCAAGGC CGTGTTTGAC TGCGTGGTGA ACTCGCTCAA GAACGTCTTC
    AACATCCTCA TCGTCTACAT GCTGTTCATG TTCATCTTCG CCGTGGTGGC CGTGCAGCTC
    TTCAAGGGCA AGTTTTTCCA CTGCACGGAT GAGTCCAAGG AGTTTGAGAA AGATTGTCGC
    GGCAAGTACC TCCTCTATGA GAAGAATGAG GTAAAGGCGA GGGATCGAGA GTGGAAGAAG
    TATGAGTTCC ATTATGACAA TGTGCTGTGG GCGCTGCTGA CCCTCTTCAC CGTGTCCACG
    GGAGAAGGCT GGCCACAGGT CCTCAAGCAC TCGGTGGACG CCACCTTTGA GAACCAGGGC
    CCCAGCCCCG GGTACCGCAT GGAGATGTCC ATCTTCTACG TCGTCTACTT TGTGGTGTTC
    CCCTTCTTCT TTGTCAACAT CTTTGTGGCC TTGATCATCA TCACCTTCCA GGAGCAGGGG
    GACAAGATGA TGGAGGAGTA CAGCCTGGAG AAAAATGAGA GGGCGTGCAT CGACTTCGCC
    ATCAGCGCCA AACCCCTGAC CCGGCACATG CCGCAGAACA AGCAGAGTTT CCAGTACCGC
    ATGTGGCAGT TCGTGGTGTC CCCGCCTTTC GAGTACACCA TCATGGCCAT GATCGCCCTC
    AACACCATCG TGCTCATGAT GAAGTTCTAC GGGGCCTCTG TTGCTTATGA GAATGCTCTG
    AGAGTATTCA ACATCGTCTT CACCTCCCTC TTCTCACTGG AATGTGTGCT GAAAGTCATG
    GCTTTTGGGA TTCTGAATTA TTTTCGCGAT GCCTGGAACA TCTTCGACTT TGTGACTGTT
    CTGGGCAGCA TCACCGACAT CCTCGTGACC GAGTTTGGGA ATAACTTCAT CAACTTGAGC
    TTCCTCCGCC TCTTCCGAGC TGCCCGGCTC ATCAAACTCC TCCGTCAGGG TTACACCATC
    CGAATTCTTC TCTGGACCTT TGTGCAGTCC TTCAAGGCCC TGCCCTATGT CTGTCTACTG
    ATTGCCATGC TTTTTTTCAT CTACGCCATC ATTGGGATGC AGGTGTTTGG CAACATTGGC
    ATCGATGTGG AGGACGAGGA CAGTGATGAA GATGAATTCC AAATCACTGA GCACAATAAC
    TTCCGCACCT TCTTCCAGGC CCTCATGCTT CTCTTCCGGA GTGCCACTGG GGAGGCTTGG
    CACAACATCA TGCTTTCCTG CCTCAGCGGG AAACCCTGTG ATAAGAACTC TGGCATCCTG
    ACTCACGAAT GCGGCAATGA ATTTGCTTAT TTTTACTTCG TTTCCTTCAT CTTCCTCTGC
    TCGTTTCTGA TGCTGAATCT TTTTGTTGCC GTCATCATGG ACAACTTTGA GTACCTCACC
    CGAGATTCCT CCATCCTGGG TCCCCACCAC CTGGATGAGT ACGTGCGTGT CTGGGCCGAG
    TATGACCCTG CAGCGTGCGG TCGGATTCAT TATAAGGATA TGTACAGTTT ATTGCGTGTA
    ATATCTCCCC CTCTCGGCTT AGGCAAGAAA TGTCCTCATA GGGTTGCTTG CAAGAGGCTC
    CTGCGGATGG ATCTGCCCGT CGCTGATGAC AACACCGTCC ACTTCAACTC TACCCTCATG
    GCTCTGATCC GCACGGCCCT GGACATCAAA ATTGCCAAAG GTGGAGCTGA CAAACAACAA
    ATGGATGCTG AGTTGCGGAA GGAGATGATG GCCATCTGGC CCAATCTGTC CCAGAAGACG
    CTGGACCTGC TGGTCACCCC CCACAAGTCC ACGGACCTGA CAGTGGGGAA GATCTATGCG
    GCCATGATGA TCATGGAGTA TTACCGGCAG AGCAAGGCCA AGAAGCTTCA GGCCATGCGC
    GAGGAACAGA ACCGGACACC TCTCATGTTC CAGCGCATGG AGCCCCCATC GCCAACACAG
    GAGGGGGGGC CTGGCCAGAA CGCCCTCCCC TCCACCCAGC TGGACCCAGG AGGAGGCTTG
    ATGGCTCACG AAAGTGGCAT GAAAGAGAGC CCATCCTGGG TGACCCAGCG GGCCCAGGAG
    ATGTTCCAGA AGACGGGCAC ATGGAGCCCA GAGCGGGGCC CCCCCACTGA TATGCCTAAT
    AGCCAACCCA ACTCTCAGTC TGTGGAGATG CGAGAGATGG GCAGAGACGG CTACTCTGAC
    AGTGAGCACT ACTTCCCCGT TGAAGGCCAG GCCCGGGCCG CCTCCATGCC CCGCCTGCCT
    GCCGAGAACC AGAGGAGAAG GGGCCGGCCA CGTGGGAATA ACCTCAGTAC CATCTCAGAC
    ACCAGCCCCA TGAAGCGCTC GGCCTCCGTG CTGGGCCCCA AGGCCCGGCG CCTGGACGAC
    TACTCCCTGG AGAGGGTGCC GCCCGAGGAG AACCAGCGGC ACCACCAGAG GCGCAGAGAC
    CGTGGCCACC GCACCTCCGA GCGCTCCTTG GGCCGCTACA CGGACGTGGA CACAGGCTTG
    GGGACAGACC TGAGCATGAC CACCCAATCT GGGGACCTGC CATCAAAGGA AAGAGACCAG
    GAGCGGGGCC GGCCCAAGGA CCGGAAGCAT CGGCAACACC ACCACCACCA CCACCACCAC
    CACCATCCCC CGCCCCCTGA CAAGGAGCGC TACACCCAGG AGCGGCCTGA GCACGGCCGG
    CCCCGGGCCC GTGACCAGCG CTGGTCCCGC TCACCCAGTG AGGGCCGGGA GCACATGGCC
    CACCGTCAGG GCAGTAGTTC CGTAAGTGGA AGCCCAGCCC CCTCAACATC TGGTACCAGC
    ACTCCGCGGC GGGGACGCCG CCAGCTCCCC CAGACCCCCT CCACTCCCCG GCCGCACGTG
    TCCTATTCCC CCGTGATCCG TAAGGCCGGC GGCTCGGGTC CCCAGCAGCA GCAGCAGCAG
    CCCGCGGCGC GGCCGGGCCG AACGGCCCCC AGCGGCCCGC GGAGGTACCC GGGCCCCGTG
    GCCGAGCCCC TGGCCGGGGA GCGGCCTCCC ACGGGAGGCC ACGGAAGCAG CCGCTCGCCC
    GCGATGGAGC GGCGGATCCC GGGCCCGGCC CGGAGCGAGT CCCCCAGGGC GCCGTGTCGC
    CACGGCGGGG CGCGATGGCC GGCGTCCGGC GCGCACGCGG CCGAGGGCCC CCCGGGCCCC
    CGGCACCACC ACGGTTACTA CCGGGGCTCC GACTACGACG AGGCCGATGG CCCGGGCGGC
    GGCGGCGGGG AGGAGGAGGA GCCCCGGGCC GCCGCCTACG ACGCGCCGCC CCCCGCGCGC
    CACGGGTGCT CGCCCAGGAC TCCCCGGGCC GCGGGCCCGG CCTGCGCCTC GCCTTCCAGG
    CACGGCCGGC GACTCCCGAA CGGCTACTAC CCGGCCCACG GACTGGCCAG GCCCCGCGGG
    CCGGGCTCCC GGAAAGGCCT GCACGAAGCC TACAGCGAGA GCGACGACGA TTGGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.