ATP2B1 cDNA ORF clone, Bos taurus(cattle)

The following ATP2B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa19045 XM_024991382.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa19045 XM_024991381.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa234188 XM_024991379.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa234189 XM_024991380.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa90695 XM_015470887.1
Latest version!
Bos taurus ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa40522 NM_174696.2
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa19044 XM_005206044.3
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa19044 XM_024991378.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa19044 XM_010804765.3
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa19044 XM_010804765.2 Bos taurus ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa19044 XM_005206044.2 Bos taurus ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa19045
    Clone ID Related Accession (Same CDS sequence) XM_024991381.1 , XM_024991382.1
    Accession Version XM_024991381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCT
    TCTACTGCTT CTACTACTGT GGGGTATTCG AGTGGTGAAT GCATTTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847149.1
    CDS621..4151
    Translation

    Target ORF information:

    RefSeq Version XM_024991381.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCT
    TCTACTGCTT CTACTACTGT GGGGTATTCG AGTGGTGAAT GCATTTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19045
    Clone ID Related Accession (Same CDS sequence) XM_024991381.1 , XM_024991382.1
    Accession Version XM_024991382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCT
    TCTACTGCTT CTACTACTGT GGGGTATTCG AGTGGTGAAT GCATTTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847150.1
    CDS645..4175
    Translation

    Target ORF information:

    RefSeq Version XM_024991382.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCT
    TCTACTGCTT CTACTACTGT GGGGTATTCG AGTGGTGAAT GCATTTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234188
    Clone ID Related Accession (Same CDS sequence) XM_024991379.1
    Accession Version XM_024991379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATTCGAGTG
    GTGAATGCAT TTCGTAGTTC TTTATATGAA GGGTTAGAAA AACCGGAATC AAGAAGTTCA
    ATTCACAACT TTATGACACA TCCTGAGTTT AGGATAGAAG ATTCAGAGCC TCATATTCCC
    CTTATTGATG ACACTGATGC TGAAGATGAT GCTCCTACAA AACGTAACTC CAGTCCTCCA
    CCCTCTCCCA ACAAAAATAA CAATGCTGTT GACAGCGGGA TTTACCTTAC AATAGAAATG
    AACAAGTCTG CTACCTCTTC ATCCCCAGGA AGCCCACTAC ATAGTTTGGA AACATCACTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847147.1
    CDS645..4307
    Translation

    Target ORF information:

    RefSeq Version XM_024991379.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATTCGAGTG
    GTGAATGCAT TTCGTAGTTC TTTATATGAA GGGTTAGAAA AACCGGAATC AAGAAGTTCA
    ATTCACAACT TTATGACACA TCCTGAGTTT AGGATAGAAG ATTCAGAGCC TCATATTCCC
    CTTATTGATG ACACTGATGC TGAAGATGAT GCTCCTACAA AACGTAACTC CAGTCCTCCA
    CCCTCTCCCA ACAAAAATAA CAATGCTGTT GACAGCGGGA TTTACCTTAC AATAGAAATG
    AACAAGTCTG CTACCTCTTC ATCCCCAGGA AGCCCACTAC ATAGTTTGGA AACATCACTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234189
    Clone ID Related Accession (Same CDS sequence) XM_024991380.1
    Accession Version XM_024991380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    CTTATTTCAA CAATTCCAAC TAGCCGTTTA AAATTCCTCA AAGAAGCTGG TCATGGCACA
    CAGAAGGAGG AAATACCCGA GGAGGAGTTA GCGGAGGATG TGGAAGAGAT TGATCACGCT
    GAGCGGGAGT TGCGGCGTGG CCAGATCTTG TGGTTTAGAG GTCTGAACAG AATCCAGACA
    CAGATGGATG TAGTGAATGC TTTCCAGAGT GGAAGTTCCA TTCAGGGGGC ACTAAGGCGG
    CAACCCTCCA TCGCCAGCCA GCATCATGAT ATTCGAGTGG TGAATGCATT TCGTAGTTCT
    TTATATGAAG GGTTAGAAAA ACCGGAATCA AGAAGTTCAA TTCACAACTT TATGACACAT
    CCTGAGTTTA GGATAGAAGA TTCAGAGCCT CATATTCCCC TTATTGATGA CACTGATGCT
    GAAGATGATG CTCCTACAAA ACGTAACTCC AGTCCTCCAC CCTCTCCCAA CAAAAATAAC
    AATGCTGTTG ACAGCGGGAT TTACCTTACA ATAGAAATGA ACAAGTCTGC TACCTCTTCA
    TCCCCAGGAA GCCCACTACA TAGTTTGGAA ACATCACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847148.1
    CDS621..4262
    Translation

    Target ORF information:

    RefSeq Version XM_024991380.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    CTTATTTCAA CAATTCCAAC TAGCCGTTTA AAATTCCTCA AAGAAGCTGG TCATGGCACA
    CAGAAGGAGG AAATACCCGA GGAGGAGTTA GCGGAGGATG TGGAAGAGAT TGATCACGCT
    GAGCGGGAGT TGCGGCGTGG CCAGATCTTG TGGTTTAGAG GTCTGAACAG AATCCAGACA
    CAGATGGATG TAGTGAATGC TTTCCAGAGT GGAAGTTCCA TTCAGGGGGC ACTAAGGCGG
    CAACCCTCCA TCGCCAGCCA GCATCATGAT ATTCGAGTGG TGAATGCATT TCGTAGTTCT
    TTATATGAAG GGTTAGAAAA ACCGGAATCA AGAAGTTCAA TTCACAACTT TATGACACAT
    CCTGAGTTTA GGATAGAAGA TTCAGAGCCT CATATTCCCC TTATTGATGA CACTGATGCT
    GAAGATGATG CTCCTACAAA ACGTAACTCC AGTCCTCCAC CCTCTCCCAA CAAAAATAAC
    AATGCTGTTG ACAGCGGGAT TTACCTTACA ATAGAAATGA ACAAGTCTGC TACCTCTTCA
    TCCCCAGGAA GCCCACTACA TAGTTTGGAA ACATCACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90695
    Clone ID Related Accession (Same CDS sequence) XM_015470887.1
    Accession Version XM_015470887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103916.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCT
    TCTACTGCTT CTACTACTGT GGGATTCGAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326373.1
    CDS478..3993
    Translation

    Target ORF information:

    RefSeq Version XM_015470887.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATGT AACAAATATT TCTACCCCTA CACATGTAGT GTTTTCCTCT
    TCTACTGCTT CTACTACTGT GGGATTCGAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40522
    Clone ID Related Accession (Same CDS sequence) NM_174696.2
    Accession Version NM_174696.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF332982.1. On or before Mar 29, 2005 this sequence version replaced XM_592068.1, NM_174696.1. ##Evidence-Data-START## Transcript exon combination :: AF332982.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGGAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CGGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCCG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGCTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATCCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATTCGAGTG
    GTGAATGCAT TTCGTAGTTC TTTATATGAA GGGTTAGAAA AACCGGAATC AAGAAGTTCA
    ATTCACAACT TTATGACACA TCCTGAGTTT AGGATAGAAG ATTCAGAGCC TCATATTCCC
    CTTATTGATG ACACTGATGC TGAAGATGAT GCTCCTACAA AACGTAACTC CAGTCCTCCA
    CCCTCTCCCA ACAAAAATAA CAATGCTGTT GACAGCGGGA TTTACCTTAC AATAGAAATG
    AACAAGTCTG CTACCTCTTC ATCCCCAGGA AGCCCACTAC ATAGTTTGGA AACATCACTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_777121.1
    CDS462..4124
    Translation

    Target ORF information:

    RefSeq Version NM_174696.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174696.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGGAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CGGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCCG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGCTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATCCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATTCGAGTG
    GTGAATGCAT TTCGTAGTTC TTTATATGAA GGGTTAGAAA AACCGGAATC AAGAAGTTCA
    ATTCACAACT TTATGACACA TCCTGAGTTT AGGATAGAAG ATTCAGAGCC TCATATTCCC
    CTTATTGATG ACACTGATGC TGAAGATGAT GCTCCTACAA AACGTAACTC CAGTCCTCCA
    CCCTCTCCCA ACAAAAATAA CAATGCTGTT GACAGCGGGA TTTACCTTAC AATAGAAATG
    AACAAGTCTG CTACCTCTTC ATCCCCAGGA AGCCCACTAC ATAGTTTGGA AACATCACTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19044
    Clone ID Related Accession (Same CDS sequence) XM_010804765.2 , XM_005206044.2 , XM_010804765.3 , XM_005206044.3 , XM_024991378.1
    Accession Version XM_010804765.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103916.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803067.1
    CDS608..4357
    Translation

    Target ORF information:

    RefSeq Version XM_010804765.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804765.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19044
    Clone ID Related Accession (Same CDS sequence) XM_010804765.2 , XM_005206044.2 , XM_010804765.3 , XM_005206044.3 , XM_024991378.1
    Accession Version XM_005206044.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103916.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005206044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206101.1
    CDS478..4227
    Translation

    Target ORF information:

    RefSeq Version XM_005206044.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19044
    Clone ID Related Accession (Same CDS sequence) XM_010804765.2 , XM_005206044.2 , XM_010804765.3 , XM_005206044.3 , XM_024991378.1
    Accession Version XM_010804765.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803067.1
    CDS645..4394
    Translation

    Target ORF information:

    RefSeq Version XM_010804765.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804765.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19044
    Clone ID Related Accession (Same CDS sequence) XM_010804765.2 , XM_005206044.2 , XM_010804765.3 , XM_005206044.3 , XM_024991378.1
    Accession Version XM_005206044.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206101.1
    CDS621..4370
    Translation

    Target ORF information:

    RefSeq Version XM_005206044.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206044.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19044
    Clone ID Related Accession (Same CDS sequence) XM_010804765.2 , XM_005206044.2 , XM_010804765.3 , XM_005206044.3 , XM_024991378.1
    Accession Version XM_024991378.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847146.1
    CDS268..4017
    Translation

    Target ORF information:

    RefSeq Version XM_024991378.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 1 (ATP2B1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGACA TGGCAAACAA CTCAGTTGCA TATGGTGGAG TAAAAAACTC TTTGAAGGAA 
    GCTAATCACG ATGGAGACTT TGGAATTACA CTCGCAGAGC TGCGGGCCCT CATGGAGCTC
    AGGTCCACAG ACGCATTACG GAAAATACAG GAAAGCTATG GAGACGTCTA TGGAATCTGC
    ACCAGGTTAA AAACATCTCC CAATGAAGGT TTAAGTGGAA ACCCTGTAGA TATAGAAAGA
    AGAGAAGCAG TATTTGGAAA GAATTTTATA CCTCCTAAAA AGCCAAAAAC CTTTCTTCAG
    CTAGTATGGG AAGCATTACA AGATGTCACT TTAATTATAT TAGAAATTGC AGCCATAGTA
    TCATTGGGCC TTTCCTTTTA TCAACCTCCA GAAGGAGATA ATGCACTTTG TGGAGACGTT
    TCTGTTGGAG AGGAAGAAGG TGAAGGTGAA ACTGGTTGGA TTGAAGGAGC GGCAATCCTC
    TTGTCAGTGG TGTGTGTGGT GTTAGTAACA GCATTTAATG ACTGGAGTAA GGAAAAGCAG
    TTCAGAGGTC TGCAGAGTCG AATTGAACAA GAACAGAAGT TCACAGTCAT CAGGGGCGGT
    CAGGTCATTC AGATACCCGT AGCTGACATT ACTGTTGGAG ATATTGCTCA AGTGAAATAT
    GGTGATCTTC TTCCCGCTGA TGGCATACTT ATTCAAGGCA ATGATCTTAA GATTGATGAA
    AGCTCATTGA CTGGTGAATC TGATCATGTT AAGAAGTCAT TAGATAAGGA TCCCTTACTT
    TTATCAGGTA CTCATGTAAT GGAAGGCTCT GGAAGAATGG TAGTTACTGC TGTAGGTATA
    AATTCTCAAA CTGGAATTAT CTTTACCTTA CTTGGAGCTG GAGGTGAAGA GGAAGAGAAG
    AAAGATGAGA AGAAAAAGGA AAAGAAAAAT AAGAAACAAG ATGGAGCTAT TGAGAATCGC
    AACAAAGCAA AAGCCCAGGA TGGTGCAGCC ATGGAAATGC AGCCTCTGAA GAGTGAAGAA
    GGTGGAGATG GTGATGAGAA AGACAAAAAG AAAGCAAATT TGCCAAAAAA GGAAAAATCT
    GTTTTACAGG GGAAACTGAC GAAACTAGCT GTTCAGATTG GCAAAGCAGG TTTGTTGATG
    TCTGCCATCA CAGTTATCAT TCTTGTCTTA TATTTTGTAA TTGATACCTT CTGGGTTCAG
    AAGAGACCAT GGCTTGCTGA GTGCACACCG ATTTACATAC AGTATTTTGT GAAGTTCTTC
    ATTATTGGAG TTACGGTTTT AGTGGTGGCA GTACCAGAAG GTCTGCCCCT TGCCGTCACT
    ATCTCACTAG CTTACTCAGT CAAGAAAATG ATGAAAGATA ATAATTTAGT AAGGCATCTG
    GATGCTTGTG AAACCATGGG AAATGCTACA GCTATTTGTT CAGATAAGAC AGGAACATTG
    ACAATGAACC GAATGACAGT TGTTCAAGCT TACATAAATG AAAAACATTA TAAAAAGATT
    CCTGACCCAG AAGCTATTCC ACCAAATATT TTGTCCTATC TTGTAACAGG AATTTCTGTG
    AATTGTGCTT ATACATCAAA AATATTGCCA CCAGAGAAAG AGGGTGGATT ACCTCGTCAT
    GTTGGTAATA AAACTGAATG TGCCTTGCTG GGATTTCTTT TGGATTTAAA GCGGGATTAT
    CAGGATGTTA GAAATGAAAT ACCGGAAGAA GCACTGTACA AAGTCTACAC CTTCAATTCT
    GTCAGAAAGT CCATGAGCAC TGTCCTGAAA AATTCAGATG GAAGTTACCG AATATTCAGC
    AAGGGTGCAT CTGAGATAAT TCTGAAAAAG TGTTTCAAAA TCTTGAGTGC TAATGGTGAG
    GCAAAAGTAT TCAGACCAAG GGACCGTGAT GATATTGTAA AAACTGTGAT TGAACCGATG
    GCATCAGAAG GCTTGAGAAC CATATGTCTT GCATTCAGAG ATTTCCCAGC AGGAGAACCA
    GAACCAGAGT GGGATAATGA AAATGATATT GTCACCGGCC TTACATGCAT TGCTGTTGTG
    GGGATTGAAG ATCCTGTGAG ACCTGAGGTG CCAGATGCAA TAAAGAAATG TCAGAGGGCT
    GGAATTACTG TGCGGATGGT CACTGGTGAT AATATTAATA CTGCTCGGGC TATTGCCACC
    AAATGTGGTA TTTTACATCC TGGGGAAGAT TTTCTTTGCC TAGAAGGTAA AGATTTCAAC
    AGGAGGATAC GAAATGAAAA AGGAGAGATT GAGCAAGAGC GGATAGACAA GATTTGGCCA
    AAGCTTCGAG TGCTTGCAAG ATCATCTCCT ACTGACAAAC ATACACTGGT TAAAGGTATA
    ATTGACAGCA CTGTTTCCGA ACAACGCCAG GTGGTAGCTG TCACTGGTGA TGGTACAAAT
    GATGGCCCGG CATTAAAGAA AGCTGATGTT GGATTTGCAA TGGGTATCGC TGGAACTGAT
    GTAGCTAAAG AAGCATCTGA TATTATTCTC ACGGATGACA ATTTTACAAG CATTGTTAAA
    GCAGTTATGT GGGGACGAAA TGTCTATGAC AGCATCTCAA AATTCCTTCA GTTCCAGCTT
    ACCGTTAATG TAGTAGCTGT GATTGTTGCT TTCACGGGTG CCTGTATTAC TCAGGACTCG
    CCACTTAAGG CTGTGCAGAT GCTATGGGTA AATCTCATCA TGGACACACT TGCTTCCCTG
    GCTCTGGCGA CCGAACCCCC CACTGAATCT CTCTTGCTTC GGAAACCGTA TGGTAGAAAT
    AAGCCTCTCA TCTCACGCAC AATGATGAAG AATATTTTGG GTCATGCATT CTATCAGCTT
    GTCGTAGTCT TCACACTCTT GTTTGCCGGA GAAAAGTTTT TTGATATTGA TAGCGGAAGA
    AACGCTCCTT TGCATGCTCC TCCATCGGAA CATTATACTA TTGTTTTTAA TACCTTTGTA
    CTGATGCAAC TTTTCAACGA AATAAATGCA CGGAAAATTC ACGGTGAAAG AAATGTGTTT
    GAAGGAATCT TTAATAATGC CATCTTCTGC ACAATTGTTT TAGGCACTTT TGTGGTACAG
    ATAATAATTG TGCAGTTTGG TGGAAAACCT TTCAGTTGTT CAGAACTTTC AATAGAACAG
    TGGCTATGGT CAATATTCCT AGGAATGGGA ACGTTACTCT GGGGCCAGCT TATTTCAACA
    ATTCCAACTA GCCGTTTAAA ATTCCTCAAA GAAGCTGGTC ATGGCACACA GAAGGAGGAA
    ATACCCGAGG AGGAGTTAGC GGAGGATGTG GAAGAGATTG ATCACGCTGA GCGGGAGTTG
    CGGCGTGGCC AGATCTTGTG GTTTAGAGGT CTGAACAGAA TCCAGACACA GATGGATGTA
    GTGAATGCTT TCCAGAGTGG AAGTTCCATT CAGGGGGCAC TAAGGCGGCA ACCCTCCATC
    GCCAGCCAGC ATCATGATAT TCGAGTGGTG AATGCATTTC GTAGTTCTTT ATATGAAGGG
    TTAGAAAAAC CGGAATCAAG AAGTTCAATT CACAACTTTA TGACACATCC TGAGTTTAGG
    ATAGAAGATT CAGAGCCTCA TATTCCCCTT ATTGATGACA CTGATGCTGA AGATGATGCT
    CCTACAAAAC GTAACTCCAG TCCTCCACCC TCTCCCAACA AAAATAACAA TGCTGTTGAC
    AGCGGGATTT ACCTTACAAT AGAAATGAAC AAGTCTGCTA CCTCTTCATC CCCAGGAAGC
    CCACTACATA GTTTGGAAAC ATCACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.