ACSM1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACSM1
Entrez Gene ID	282576
Full Name	acyl-CoA synthetase medium chain family member 1
Synonyms	BUCS1
General protein information	Preferred Names acyl-CoA synthetase medium chain family member 1 Names acyl-coenzyme A synthetase ACSM1, mitochondrial XL-III XM-ligase 3 butyrate--CoA ligase 1 butyryl Coenzyme A synthetase 1 butyryl-coenzyme A synthetase 1 lipoate-activating enzyme middle-chain acyl-CoA synthetase 1 xenobiotic/medium-chain fatty acid:CoA ligase XL-3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005224691.3	XP_005224748.1	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
XM_005224692.3	XP_005224749.1	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
XM_005224692.4	XP_005224749.1	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
XM_015460313.2	XP_015315799.1	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
XM_015460313.1	XP_015315799.1	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
XM_005224691.4	XP_005224748.1	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
NM_174682.2	NP_777107.1	acyl-coenzyme A synthetase ACSM1, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACSM1	NP_443188.2
Rattus norvegicus (Norway rat)	Acsm1	NP_001101972.2
Mus musculus (house mouse)	Acsm1	NP_473435.1
Pan troglodytes (chimpanzee)	ACSM1	XP_003954530.1
	ACSM1	XP_001089283.1
Bos taurus (cattle)	ACSM1	NP_777107.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Purification, characterization, and cDNA cloning of lipoate-activating enzyme from bovine liver.
Fujiwara K, Takeuchi S, Okamura-Ikeda K, Motokawa Y
The Journal of biological chemistry276(31)28819-23(2001 Aug)

Isolation and sequencing of cDNAs for the XL-I and XL-III forms of bovine liver xenobiotic-metabolizing medium-chain fatty acid:CoA ligase.
Vessey DA, Lau E, Kelley M
Journal of biochemical and molecular toxicology14(1)11-9(2000)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACSM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41479	NM_174682.2 Latest version!	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32943	XM_015460313.2 Latest version!	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32943	XM_005224691.4 Latest version!	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32943	XM_005224692.4 Latest version!	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa32943	XM_005224692.3	Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32943	XM_015460313.1	Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32943	XM_005224691.3	Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACSM1 ( NM_174682.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777107.1 Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), mRNA.

Price & Availability↑

CloneID	OBa41479
Clone ID Related Accession (Same CDS sequence)	NM_174682.2
Accession Version	NM_174682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB048289.1. On Jun 3, 2003 this sequence version replaced NM_174682.1. ##Evidence-Data-START## Transcript exon combination :: AB048289.1, BC109602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTG CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777107.1
CDS	131..1864
Translation	MQRLMKFRVLWGIHMSCPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	NM_174682.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174682.2

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTG CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSM1 ( XM_005224691.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224748.1 Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32943
Clone ID Related Accession (Same CDS sequence)	XM_005224691.3 , XM_005224692.3 , XM_005224692.4 , XM_015460313.2 , XM_015460313.1 , XM_005224691.4
Accession Version	XM_005224691.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224748.1
CDS	202..1935
Translation	MQRLMKFRVLWGIHMSFPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	XM_005224691.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224691.3

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSM1 ( XM_005224692.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224749.1 Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32943
Clone ID Related Accession (Same CDS sequence)	XM_005224691.3 , XM_005224692.3 , XM_005224692.4 , XM_015460313.2 , XM_015460313.1 , XM_005224691.4
Accession Version	XM_005224692.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224749.1
CDS	241..1974
Translation	MQRLMKFRVLWGIHMSFPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	XM_005224692.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224692.3

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSM1 ( XM_005224692.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224749.1 Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32943
Clone ID Related Accession (Same CDS sequence)	XM_005224691.3 , XM_005224692.3 , XM_005224692.4 , XM_015460313.2 , XM_015460313.1 , XM_005224691.4
Accession Version	XM_005224692.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224692.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224749.1
CDS	191..1924
Translation	MQRLMKFRVLWGIHMSFPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	XM_005224692.4
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224692.4

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSM1 ( XM_015460313.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315799.1 Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa32943
Clone ID Related Accession (Same CDS sequence)	XM_005224691.3 , XM_005224692.3 , XM_005224692.4 , XM_015460313.2 , XM_015460313.1 , XM_005224691.4
Accession Version	XM_015460313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315799.1
CDS	213..1946
Translation	MQRLMKFRVLWGIHMSFPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	XM_015460313.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460313.2

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSM1 ( XM_015460313.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315799.1 Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa32943
Clone ID Related Accession (Same CDS sequence)	XM_005224691.3 , XM_005224692.3 , XM_005224692.4 , XM_015460313.2 , XM_015460313.1 , XM_005224691.4
Accession Version	XM_015460313.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315799.1
CDS	263..1996
Translation	MQRLMKFRVLWGIHMSFPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	XM_015460313.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium-chain family member 1 (ACSM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460313.1

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSM1 ( XM_005224691.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224748.1 Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X1, mRNA.

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CloneID	OBa32943
Clone ID Related Accession (Same CDS sequence)	XM_005224691.3 , XM_005224692.3 , XM_005224692.4 , XM_015460313.2 , XM_015460313.1 , XM_005224691.4
Accession Version	XM_005224691.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acyl-coenzyme A synthetase ACSM1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224691.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224748.1
CDS	338..2071
Translation	MQRLMKFRVLWGIHMSFPGFHHAPQHLRCRSLSGAGTLRWNDYDRPEEFNFASDVLDHWTQMEKEGKRSPNPALWWVNDQGDEVKWSFREMTDLTCRTANVLTQTCGLQTGDRLALILPRVPEWWLVCVGCIRTGIIFMPGTTQMKAKDILYRLQVSGAKAIVTTDTLAPEVESVAPECPSLKTKLLVSDHSREGWLDFRSLVKSASPDHICIKSKTLDPMAIFFTSGTTGFPKMAKHSHGFALRSYFPACRKLLQLKMSDVFWCLSDTGWILAALGSLLEPWTAGSTVFAHHLPQFDPKVIIETFFKYPITQCLAAPSVYRMILQQNYTSLRFPTLEHCCTGGEALLPEEQEQWKRQTGVLLYQAYGQSEAGISCGTLRGMKIKPGSMGKAIPPFDIQIIDDKGNIQPPNTEGNIGIRIKPTRPIGLFMYYENNPEKTAEVECGDFYNTGDRATIDEEGYFWFLGRSDDVINASGYRVGPAEVENALAEHPAVAESAVVSSPDPVRGEVVKAFIVLNPEFSSRDPGELTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIKRSELRKKEFGQK

Target ORF information:

RefSeq Version	XM_005224691.4
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224691.4

ORF Insert Sequence:

ATGCAAAGGC TGATGAAGTT TCGGGTCCTC TGGGGCATCC ACATGTCCTT CCCTGGCTTC 
CACCATGCCC CTCAGCACCT GCGCTGCCGA TCTTTATCAG GAGCTGGAAC CCTAAGATGG 
AATGACTATG ACAGACCTGA GGAATTTAAC TTTGCAAGTG ATGTACTGGA CCACTGGACT 
CAAATGGAGA AGGAGGGCAA GAGAAGTCCA AACCCAGCCC TGTGGTGGGT GAACGATCAA 
GGGGATGAAG TGAAGTGGAG CTTCAGAGAG ATGACAGACT TAACCTGTCG CACAGCCAAC 
GTCCTGACAC AGACCTGCGG CCTTCAGACG GGAGACCGTC TAGCGTTGAT TTTGCCTCGA 
GTGCCTGAGT GGTGGCTTGT GTGTGTGGGC TGCATCCGAA CAGGGATCAT CTTCATGCCA 
GGGACCACCC AGATGAAGGC CAAGGACATT CTCTACCGAC TACAAGTGTC TGGAGCCAAA 
GCCATCGTGA CCACAGATAC CCTTGCCCCA GAGGTGGAGT CTGTGGCACC TGAGTGTCCT 
TCTCTGAAAA CCAAGCTCCT GGTGTCTGAC CACAGCCGTG AAGGGTGGCT GGACTTCCGA 
TCACTGGTTA AATCAGCCTC CCCAGATCAC ATCTGTATTA AGTCAAAGAC CCTGGACCCA 
ATGGCCATCT TCTTCACCAG TGGGACCACG GGCTTCCCCA AGATGGCAAA GCACAGCCAC 
GGATTCGCTT TACGATCCTA TTTCCCAGCA TGCAGGAAAT TGTTGCAGCT GAAGATGTCT 
GATGTCTTCT GGTGCCTGTC AGACACAGGC TGGATTCTGG CTGCCTTGGG GTCCCTGCTT 
GAACCATGGA CAGCGGGGTC TACAGTCTTT GCTCATCATC TGCCTCAGTT TGACCCTAAG 
GTCATTATAG AGACATTTTT CAAATATCCA ATTACCCAAT GCCTTGCTGC ACCTTCTGTA 
TATCGAATGA TTTTGCAGCA GAATTACACC AGTCTTAGGT TCCCCACTCT GGAACACTGC 
TGTACTGGTG GGGAAGCCCT GCTGCCCGAG GAGCAAGAAC AGTGGAAAAG ACAGACAGGT 
GTTCTGCTCT ACCAGGCCTA TGGACAGTCA GAAGCGGGAA TAAGCTGTGG CACTCTCCGG 
GGGATGAAGA TCAAGCCAGG TTCCATGGGG AAGGCCATCC CACCCTTTGA CATTCAGATC 
ATTGATGACA AGGGCAATAT CCAGCCTCCC AACACGGAAG GAAACATTGG CATCAGGATC 
AAGCCCACCA GGCCCATAGG CCTCTTCATG TACTATGAGA ATAACCCAGA GAAAACAGCT 
GAAGTGGAAT GTGGGGACTT TTATAACACT GGGGACAGAG CAACTATTGA CGAAGAGGGC 
TACTTCTGGT TCCTTGGGAG GAGTGACGAT GTCATCAATG CATCCGGATA CCGCGTTGGG 
CCAGCAGAAG TAGAGAATGC CCTGGCAGAG CACCCAGCGG TGGCTGAGTC TGCTGTGGTG 
AGCAGCCCGG ACCCGGTTCG AGGCGAGGTA GTGAAGGCAT TTATTGTCCT GAACCCGGAA 
TTCTCGAGCC GTGACCCAGG CGAGCTCACC AAGGAGCTGC AGCAGCATGT CAAGTCAGTG 
ACAGCCCCAT ACAAGTACCC GAGGAAGGTG GAGTTTGTCT CAGAGCTGCC CAAAACCATC 
ACTGGCAAGA TAAAGAGAAG TGAGCTTCGG AAGAAGGAGT TTGGTCAGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Purification, characterization, and cDNA cloning of lipoate-activating enzyme from bovine liver.
The Journal of biological chemistry276(31)28819-23(2001 Aug)
Fujiwara K,Takeuchi S,Okamura-Ikeda K,Motokawa Y

Isolation and sequencing of cDNAs for the XL-I and XL-III forms of bovine liver xenobiotic-metabolizing medium-chain fatty acid:CoA ligase.
Journal of biochemical and molecular toxicology14(1)11-9(2000)
Vessey DA,Lau E,Kelley M

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ACSM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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