KCNMA1 cDNA ORF clone, Bos taurus(cattle)

The following KCNMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231561 XM_024986562.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OBa231556 XM_024986557.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231557 XM_024986558.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231558 XM_024986559.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231559 XM_024986560.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa231560 XM_024986561.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa231562 XM_024986563.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231563 XM_024986564.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231564 XM_024986566.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231565 XM_024986567.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231566 XM_024986568.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231567 XM_024986569.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231568 XM_024986570.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231569 XM_024986571.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231570 XM_024986572.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa231571 XM_024986573.1
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa43733 NM_174680.2
Latest version!
Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231561
    Clone ID Related Accession (Same CDS sequence) XM_024986562.1
    Accession Version XM_024986562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACTCT TCCTGGCCAA GAGAGAAAAG AGCTTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842330.1
    CDS179..778
    Translation

    Target ORF information:

    RefSeq Version XM_024986562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACTCT TCCTGGCCAA GAGAGAAAAG AGCTTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231556
    Clone ID Related Accession (Same CDS sequence) XM_024986557.1
    Accession Version XM_024986557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTCAA GGTTGGAGCT AGATCACGCT ATCCCAAAGA CCCATTTGAG
    TTCAAGAACA CAGCTCACAA TTCTCAGCTT GTGACCGAGC CAGTTGAAGA TGAACAGCCG
    TCGACACTGT CACCCAAAAA AAAGCAGCGC AACGGAGGCA TGCGGAACTC ACCCAGCTCG
    TCGCCCAAGC TGATGAGGCA TGACCCCTTG TTAATTCCTG GCAATGATCA GATTGACAAC
    ATGGACTCCA ATGTGAAGAA ATATGACTCT ACTGGGATGT TTCACTGGTG TGCGCCCAAG
    GAGATAGAGA AAGTCATCCT GACCCGAAGT GAAGCTGCCA TGACCGTCCT GAGCGGCCAC
    GTGGTGGTCT GCATCTTTGG CGACGTCAGC TCTGCCCTGA TTGGCCTTCG GAACCTGGTG
    ATGCCGCTCC GTGCCAGCAA CTTTCACTAC CACGAGCTCA AGCACATCGT GTTTGTGGGC
    TCCATCGAGT ACCTCAAGCG GGAATGGGAG ACACTGCATA ACTTCCCCAA GGTTTCCATC
    TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCCG TCAACATCAA CCTCTGTGAC
    ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG ATACTTCGCT GCAGGACAAG
    GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT TTGATGACAG CATCGGGGTC
    TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG ACCGATCCTC ACCAGATAAC
    AGCCCAGTAC ATGGGATGTT ACGCCAGCCC TCCATCACGA CTGGGGTCAA CATCCCCATC
    ATCACTGAAC TAGTGAACGA TACTAATGTT CAGTTTTTGG ACCAAGACGA TGACGATGAC
    CCCGACACAG AACTGTACCT CACGCAGCCG TTTGCATGTG GGACAGCGTT CGCCGTCAGT
    GTCCTGGATT CACTCATGAG CGCAACATAC TTCAATGACA ACATCCTTAC CCTGATACGG
    ACCCTGGTGA CTGGAGGAGC CACACCAGAG CTGGAGGCTT TGATCGCTGA GGAGAATGCA
    CTCCGCGGGG GCTACAGCAC CCCCCAGACG CTGGCCAACA GGGACCGCTG CCGTGTGGCG
    CAGTTAGCGC TGCTGGACGG GCCCTTTGCG GACCTGGGGG ATGGTGGTTG TTATGGTGAT
    CTGTTCTGCA AAGCTCTGAA AACATACAAT ATGCTTTGTT TTGGAATTTA TCGGCTGAGA
    GATGCTCACC TCAGCACCCC CAGCCAGTGC ACGAAGAGGT ATGTCATCAC CAACCCCCCG
    TACGAGTTTG AGCTCGTGCC GACGGACCTG ATCTTCTGCT TAATGCAGTT TGACCACAAC
    GCCGGCCAGT CCCGGGCCAG CCTCTCCCAT TCCTCCCACT CGTCCCAGTC CTCTAGCAAG
    AAGAGCTCCT CCGTCCACTC CATCCCATCC ACGGCAAACC GACAGAACCG GCCCAAGTCC
    CGGGAGTCCC GAGACAAACA GAACAGAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842325.1
    CDS1..3588
    Translation

    Target ORF information:

    RefSeq Version XM_024986557.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTCAA GGTTGGAGCT AGATCACGCT ATCCCAAAGA CCCATTTGAG
    TTCAAGAACA CAGCTCACAA TTCTCAGCTT GTGACCGAGC CAGTTGAAGA TGAACAGCCG
    TCGACACTGT CACCCAAAAA AAAGCAGCGC AACGGAGGCA TGCGGAACTC ACCCAGCTCG
    TCGCCCAAGC TGATGAGGCA TGACCCCTTG TTAATTCCTG GCAATGATCA GATTGACAAC
    ATGGACTCCA ATGTGAAGAA ATATGACTCT ACTGGGATGT TTCACTGGTG TGCGCCCAAG
    GAGATAGAGA AAGTCATCCT GACCCGAAGT GAAGCTGCCA TGACCGTCCT GAGCGGCCAC
    GTGGTGGTCT GCATCTTTGG CGACGTCAGC TCTGCCCTGA TTGGCCTTCG GAACCTGGTG
    ATGCCGCTCC GTGCCAGCAA CTTTCACTAC CACGAGCTCA AGCACATCGT GTTTGTGGGC
    TCCATCGAGT ACCTCAAGCG GGAATGGGAG ACACTGCATA ACTTCCCCAA GGTTTCCATC
    TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCCG TCAACATCAA CCTCTGTGAC
    ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG ATACTTCGCT GCAGGACAAG
    GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT TTGATGACAG CATCGGGGTC
    TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG ACCGATCCTC ACCAGATAAC
    AGCCCAGTAC ATGGGATGTT ACGCCAGCCC TCCATCACGA CTGGGGTCAA CATCCCCATC
    ATCACTGAAC TAGTGAACGA TACTAATGTT CAGTTTTTGG ACCAAGACGA TGACGATGAC
    CCCGACACAG AACTGTACCT CACGCAGCCG TTTGCATGTG GGACAGCGTT CGCCGTCAGT
    GTCCTGGATT CACTCATGAG CGCAACATAC TTCAATGACA ACATCCTTAC CCTGATACGG
    ACCCTGGTGA CTGGAGGAGC CACACCAGAG CTGGAGGCTT TGATCGCTGA GGAGAATGCA
    CTCCGCGGGG GCTACAGCAC CCCCCAGACG CTGGCCAACA GGGACCGCTG CCGTGTGGCG
    CAGTTAGCGC TGCTGGACGG GCCCTTTGCG GACCTGGGGG ATGGTGGTTG TTATGGTGAT
    CTGTTCTGCA AAGCTCTGAA AACATACAAT ATGCTTTGTT TTGGAATTTA TCGGCTGAGA
    GATGCTCACC TCAGCACCCC CAGCCAGTGC ACGAAGAGGT ATGTCATCAC CAACCCCCCG
    TACGAGTTTG AGCTCGTGCC GACGGACCTG ATCTTCTGCT TAATGCAGTT TGACCACAAC
    GCCGGCCAGT CCCGGGCCAG CCTCTCCCAT TCCTCCCACT CGTCCCAGTC CTCTAGCAAG
    AAGAGCTCCT CCGTCCACTC CATCCCATCC ACGGCAAACC GACAGAACCG GCCCAAGTCC
    CGGGAGTCCC GAGACAAACA GAACAGAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231557
    Clone ID Related Accession (Same CDS sequence) XM_024986558.1
    Accession Version XM_024986558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTCAA GGTTGGAGCT AGATCACGCT ATCCCAAAGA CCCATTTGAG
    TTCAAGAACA CAGCTCACAA TTCTCAGCTT GTGACCGAGC CAGTTGAAGA TGAACAGCCG
    TCGACACTGT CACCCAAAAA AAAGCAGCGC AACGGAGGCA TGCGGAACTC ACCCAGCTCG
    TCGCCCAAGC TGATGAGGCA TGACCCCTTG TTAATTCCTG GCAATGATCA GATTGACAAC
    ATGGACTCCA ATGTGAAGAA ATATGACTCT ACTGGGATGT TTCACTGGTG TGCGCCCAAG
    GAGATAGAGA AAGTCATCCT GACCCGAAGT GAAGCTGCCA TGACCGTCCT GAGCGGCCAC
    GTGGTGGTCT GCATCTTTGG CGACGTCAGC TCTGCCCTGA TTGGCCTTCG GAACCTGGTG
    ATGCCGCTCC GTGCCAGCAA CTTTCACTAC CACGAGCTCA AGCACATCGT GTTTGTGGGC
    TCCATCGAGT ACCTCAAGCG GGAATGGGAG ACACTGCATA ACTTCCCCAA GGTTTCCATC
    TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCCG TCAACATCAA CCTCTGTGAC
    ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG ATACTTCGCT GCAGGACAAG
    GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT TTGATGACAG CATCGGGGTC
    TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG ACCGATCCTC ACCAGATAAC
    AGCCCAGTAC ATGGGATGTT ACGCCAGCCC TCCATCACGA CTGGGGTCAA CATCCCCATC
    ATCACTGAAC TAGTGAACGA TACTAATGTT CAGTTTTTGG ACCAAGACGA TGACGATGAC
    CCCGACACAG AACTGTACCT CACGCAGCCG TTTGCATGTG GGACAGCGTT CGCCGTCAGT
    GTCCTGGATT CACTCATGAG CGCAACATAC TTCAATGACA ACATCCTTAC CCTGATACGG
    ACCCTGGTGA CTGGAGGAGC CACACCAGAG CTGGAGGCTT TGATCGCTGA GGAGAATGCA
    CTCCGCGGGG GCTACAGCAC CCCCCAGACG CTGGCCAACA GGGACCGCTG CCGTGTGGCG
    CAGTTAGCGC TGCTGGACGG GCCCTTTGCG GACCTGGGGG ATGGTGGTTG TTATGGTGAT
    CTGTTCTGCA AAGCTCTGAA AACATACAAT ATGCTTTGTT TTGGAATTTA TCGGCTGAGA
    GATGCTCACC TCAGCACCCC CAGCCAGTGC ACGAAGAGGT ATGTCATCAC CAACCCCCCG
    TACGAGTTTG AGCTCGTGCC GACGGACCTG ATCTTCTGCT TAATGCAGTT TGACCACAAC
    GCCGGCCAGT CCCGGGCCAG CCTCTCCCAT TCCTCCCACT CGTCCCAGTC CTCTAGCAAG
    AAGAGCTCCT CCGTCCACTC CATCCCATCC ACGGCAAACC GACAGAACCG GCCCAAGTCC
    CGGGAGTCCC GAGACAAACA GAAAAAAGAA ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842326.1
    CDS1..3585
    Translation

    Target ORF information:

    RefSeq Version XM_024986558.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTCAA GGTTGGAGCT AGATCACGCT ATCCCAAAGA CCCATTTGAG
    TTCAAGAACA CAGCTCACAA TTCTCAGCTT GTGACCGAGC CAGTTGAAGA TGAACAGCCG
    TCGACACTGT CACCCAAAAA AAAGCAGCGC AACGGAGGCA TGCGGAACTC ACCCAGCTCG
    TCGCCCAAGC TGATGAGGCA TGACCCCTTG TTAATTCCTG GCAATGATCA GATTGACAAC
    ATGGACTCCA ATGTGAAGAA ATATGACTCT ACTGGGATGT TTCACTGGTG TGCGCCCAAG
    GAGATAGAGA AAGTCATCCT GACCCGAAGT GAAGCTGCCA TGACCGTCCT GAGCGGCCAC
    GTGGTGGTCT GCATCTTTGG CGACGTCAGC TCTGCCCTGA TTGGCCTTCG GAACCTGGTG
    ATGCCGCTCC GTGCCAGCAA CTTTCACTAC CACGAGCTCA AGCACATCGT GTTTGTGGGC
    TCCATCGAGT ACCTCAAGCG GGAATGGGAG ACACTGCATA ACTTCCCCAA GGTTTCCATC
    TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCCG TCAACATCAA CCTCTGTGAC
    ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG ATACTTCGCT GCAGGACAAG
    GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT TTGATGACAG CATCGGGGTC
    TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG ACCGATCCTC ACCAGATAAC
    AGCCCAGTAC ATGGGATGTT ACGCCAGCCC TCCATCACGA CTGGGGTCAA CATCCCCATC
    ATCACTGAAC TAGTGAACGA TACTAATGTT CAGTTTTTGG ACCAAGACGA TGACGATGAC
    CCCGACACAG AACTGTACCT CACGCAGCCG TTTGCATGTG GGACAGCGTT CGCCGTCAGT
    GTCCTGGATT CACTCATGAG CGCAACATAC TTCAATGACA ACATCCTTAC CCTGATACGG
    ACCCTGGTGA CTGGAGGAGC CACACCAGAG CTGGAGGCTT TGATCGCTGA GGAGAATGCA
    CTCCGCGGGG GCTACAGCAC CCCCCAGACG CTGGCCAACA GGGACCGCTG CCGTGTGGCG
    CAGTTAGCGC TGCTGGACGG GCCCTTTGCG GACCTGGGGG ATGGTGGTTG TTATGGTGAT
    CTGTTCTGCA AAGCTCTGAA AACATACAAT ATGCTTTGTT TTGGAATTTA TCGGCTGAGA
    GATGCTCACC TCAGCACCCC CAGCCAGTGC ACGAAGAGGT ATGTCATCAC CAACCCCCCG
    TACGAGTTTG AGCTCGTGCC GACGGACCTG ATCTTCTGCT TAATGCAGTT TGACCACAAC
    GCCGGCCAGT CCCGGGCCAG CCTCTCCCAT TCCTCCCACT CGTCCCAGTC CTCTAGCAAG
    AAGAGCTCCT CCGTCCACTC CATCCCATCC ACGGCAAACC GACAGAACCG GCCCAAGTCC
    CGGGAGTCCC GAGACAAACA GAAAAAAGAA ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231558
    Clone ID Related Accession (Same CDS sequence) XM_024986559.1
    Accession Version XM_024986559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAACAGA
    AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842327.1
    CDS1..3501
    Translation

    Target ORF information:

    RefSeq Version XM_024986559.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAACAGA
    AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231559
    Clone ID Related Accession (Same CDS sequence) XM_024986560.1
    Accession Version XM_024986560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAAAAAA
    GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842328.1
    CDS366..3863
    Translation

    Target ORF information:

    RefSeq Version XM_024986560.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAAAAAA
    GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231560
    Clone ID Related Accession (Same CDS sequence) XM_024986561.1
    Accession Version XM_024986561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTCAA GGTTGGAGCT AGATCACGCT ATCCCAAAGA CCCATTTGAG
    TTCAAGAACA CAGCTCACAA TTCTCAGCTT GTGACCGAGC CAGTTGAAGA TGAACAGCCG
    TCGACACTGT CACCCAAAAA AAAGCAGCGC AACGGAGGCA TGCGGAACTC ACCCAGCTCG
    TCGCCCAAGC TGATGAGGCA TGACCCCTTG TTAATTCCTG GCAATGATCA GATTGACAAC
    ATGGACTCCA ATGTGAAGAA ATATGACTCT ACTGGGATGT TTCACTGGTG TGCGCCCAAG
    GAGATAGAGA AAGTCATCCT GACCCGAAGT GAAGCTGCCA TGACCGTCCT GAGCGGCCAC
    GTGGTGGTCT GCATCTTTGG CGACGTCAGC TCTGCCCTGA TTGGCCTTCG GAACCTGGTG
    ATGCCGCTCC GTGCCAGCAA CTTTCACTAC CACGAGCTCA AGCACATCGT GTTTGTGGGC
    TCCATCGAGT ACCTCAAGCG GGAATGGGAG ACACTGCATA ACTTCCCCAA GGTTTCCATC
    TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCCG TCAACATCAA CCTCTGTGAC
    ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG ATACTTCGCT GCAGGACAAG
    GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT TTGATGACAG CATCGGGGTC
    TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG ACCGATCCTC ACCAGATAAC
    AGCCCAGTAC ATGGGATGTT ACGCCAGCCC TCCATCACGA CTGGGGTCAA CATCCCCATC
    ATCACTGAAC TAGCTAAACC TGGCAAGTTG CCTTTGGTAT CAGTCAATCA GGAAAAAAAC
    AGTGGGACGC ACATTCTAAT GATAACCGAA CTGGGTAAGA AGCACTGTTT TCTTTCACAT
    CAGTATCTCC TTTCGTTTGT GGTTGGTGTT TCCGACTATC ACCAGCTTGT TTTTTATTTC
    ACCGGCTGTA GCAGAACGGC TACCCCATGG CACTGCTTTA AATGTGTTTT ATTTGTGGCT
    CTGGTTGGAA TCGACTGTCT TGTTCTGGCC TTTCTTAATG ATTTTTTCCC CACACAGCAT
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842329.1
    CDS1..3186
    Translation

    Target ORF information:

    RefSeq Version XM_024986561.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTCAA GGTTGGAGCT AGATCACGCT ATCCCAAAGA CCCATTTGAG
    TTCAAGAACA CAGCTCACAA TTCTCAGCTT GTGACCGAGC CAGTTGAAGA TGAACAGCCG
    TCGACACTGT CACCCAAAAA AAAGCAGCGC AACGGAGGCA TGCGGAACTC ACCCAGCTCG
    TCGCCCAAGC TGATGAGGCA TGACCCCTTG TTAATTCCTG GCAATGATCA GATTGACAAC
    ATGGACTCCA ATGTGAAGAA ATATGACTCT ACTGGGATGT TTCACTGGTG TGCGCCCAAG
    GAGATAGAGA AAGTCATCCT GACCCGAAGT GAAGCTGCCA TGACCGTCCT GAGCGGCCAC
    GTGGTGGTCT GCATCTTTGG CGACGTCAGC TCTGCCCTGA TTGGCCTTCG GAACCTGGTG
    ATGCCGCTCC GTGCCAGCAA CTTTCACTAC CACGAGCTCA AGCACATCGT GTTTGTGGGC
    TCCATCGAGT ACCTCAAGCG GGAATGGGAG ACACTGCATA ACTTCCCCAA GGTTTCCATC
    TTGCCTGGTA CGCCATTAAG TCGGGCTGAT TTAAGGGCCG TCAACATCAA CCTCTGTGAC
    ATGTGCGTTA TCCTGTCAGC CAATCAGAAT AATATTGATG ATACTTCGCT GCAGGACAAG
    GAATGCATCT TGGCGTCACT CAACATCAAA TCTATGCAGT TTGATGACAG CATCGGGGTC
    TTGCAGGCTA ATTCCCAAGG GTTCACACCT CCAGGAATGG ACCGATCCTC ACCAGATAAC
    AGCCCAGTAC ATGGGATGTT ACGCCAGCCC TCCATCACGA CTGGGGTCAA CATCCCCATC
    ATCACTGAAC TAGCTAAACC TGGCAAGTTG CCTTTGGTAT CAGTCAATCA GGAAAAAAAC
    AGTGGGACGC ACATTCTAAT GATAACCGAA CTGGGTAAGA AGCACTGTTT TCTTTCACAT
    CAGTATCTCC TTTCGTTTGT GGTTGGTGTT TCCGACTATC ACCAGCTTGT TTTTTATTTC
    ACCGGCTGTA GCAGAACGGC TACCCCATGG CACTGCTTTA AATGTGTTTT ATTTGTGGCT
    CTGGTTGGAA TCGACTGTCT TGTTCTGGCC TTTCTTAATG ATTTTTTCCC CACACAGCAT
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231562
    Clone ID Related Accession (Same CDS sequence) XM_024986563.1
    Accession Version XM_024986563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCCCAA GATGTCCATC TACAAGAGAA TGAGACGGGC ATGTTGTTTT
    GATTGCGGAC GTTCTGAGCG TGACTGCTCG TGCATGTCAG GCCGTGTGCG TGGTAACGTG
    GACACCCTTG AGAGAGCCTT CCCACTTTCT TCTGTCTCTG TTAGTGATTG CTCCACCACT
    TTCCGTGCCT TTGAAGATGA ACAGCCGTCG ACACTGTCAC CCAAAAAAAA GCAGCGCAAC
    GGAGGCATGC GGAACTCACC CAGCTCGTCG CCCAAGCTGA TGAGGCATGA CCCCTTGTTA
    ATTCCTGGCA ATGATCAGAT TGACAACATG GACTCCAATG TGAAGAAATA TGACTCTACT
    GGGATGTTTC ACTGGTGTGC GCCCAAGGAG ATAGAGAAAG TCATCCTGAC CCGAAGTGAA
    GCTGCCATGA CCGTCCTGAG CGGCCACGTG GTGGTCTGCA TCTTTGGCGA CGTCAGCTCT
    GCCCTGATTG GCCTTCGGAA CCTGGTGATG CCGCTCCGTG CCAGCAACTT TCACTACCAC
    GAGCTCAAGC ACATCGTGTT TGTGGGCTCC ATCGAGTACC TCAAGCGGGA ATGGGAGACA
    CTGCATAACT TCCCCAAGGT TTCCATCTTG CCTGGTACGC CATTAAGTCG GGCTGATTTA
    AGGGCCGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT
    ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT
    ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGGTT CACACCTCCA
    GGAATGGACC GATCCTCACC AGATAACAGC CCAGTACATG GGATGTTACG CCAGCCCTCC
    ATCACGACTG GGGTCAACAT CCCCATCATC ACTGAACTAG TGAACGATAC TAATGTTCAG
    TTTTTGGACC AAGACGATGA CGATGACCCC GACACAGAAC TGTACCTCAC GCAGCCGTTT
    GCATGTGGGA CAGCGTTCGC CGTCAGTGTC CTGGATTCAC TCATGAGCGC AACATACTTC
    AATGACAACA TCCTTACCCT GATACGGACC CTGGTGACTG GAGGAGCCAC ACCAGAGCTG
    GAGGCTTTGA TCGCTGAGGA GAATGCACTC CGCGGGGGCT ACAGCACCCC CCAGACGCTG
    GCCAACAGGG ACCGCTGCCG TGTGGCGCAG TTAGCGCTGC TGGACGGGCC CTTTGCGGAC
    CTGGGGGATG GTGGTTGTTA TGGTGATCTG TTCTGCAAAG CTCTGAAAAC ATACAATATG
    CTTTGTTTTG GAATTTATCG GCTGAGAGAT GCTCACCTCA GCACCCCCAG CCAGTGCACG
    AAGAGGTATG TCATCACCAA CCCCCCGTAC GAGTTTGAGC TCGTGCCGAC GGACCTGATC
    TTCTGCTTAA TGCAGTTTGA CCACAACGCC GGCCAGTCCC GGGCCAGCCT CTCCCATTCC
    TCCCACTCGT CCCAGTCCTC TAGCAAGAAG AGCTCCTCCG TCCACTCCAT CCCATCCACG
    GCAAACCGAC AGAACCGGCC CAAGTCCCGG GAGTCCCGAG ACAAACAGAA CAGAAAAGAA
    ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842331.1
    CDS169..3843
    Translation

    Target ORF information:

    RefSeq Version XM_024986563.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCCCAA GATGTCCATC TACAAGAGAA TGAGACGGGC ATGTTGTTTT
    GATTGCGGAC GTTCTGAGCG TGACTGCTCG TGCATGTCAG GCCGTGTGCG TGGTAACGTG
    GACACCCTTG AGAGAGCCTT CCCACTTTCT TCTGTCTCTG TTAGTGATTG CTCCACCACT
    TTCCGTGCCT TTGAAGATGA ACAGCCGTCG ACACTGTCAC CCAAAAAAAA GCAGCGCAAC
    GGAGGCATGC GGAACTCACC CAGCTCGTCG CCCAAGCTGA TGAGGCATGA CCCCTTGTTA
    ATTCCTGGCA ATGATCAGAT TGACAACATG GACTCCAATG TGAAGAAATA TGACTCTACT
    GGGATGTTTC ACTGGTGTGC GCCCAAGGAG ATAGAGAAAG TCATCCTGAC CCGAAGTGAA
    GCTGCCATGA CCGTCCTGAG CGGCCACGTG GTGGTCTGCA TCTTTGGCGA CGTCAGCTCT
    GCCCTGATTG GCCTTCGGAA CCTGGTGATG CCGCTCCGTG CCAGCAACTT TCACTACCAC
    GAGCTCAAGC ACATCGTGTT TGTGGGCTCC ATCGAGTACC TCAAGCGGGA ATGGGAGACA
    CTGCATAACT TCCCCAAGGT TTCCATCTTG CCTGGTACGC CATTAAGTCG GGCTGATTTA
    AGGGCCGTCA ACATCAACCT CTGTGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT
    ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT
    ATGCAGTTTG ATGACAGCAT CGGGGTCTTG CAGGCTAATT CCCAAGGGTT CACACCTCCA
    GGAATGGACC GATCCTCACC AGATAACAGC CCAGTACATG GGATGTTACG CCAGCCCTCC
    ATCACGACTG GGGTCAACAT CCCCATCATC ACTGAACTAG TGAACGATAC TAATGTTCAG
    TTTTTGGACC AAGACGATGA CGATGACCCC GACACAGAAC TGTACCTCAC GCAGCCGTTT
    GCATGTGGGA CAGCGTTCGC CGTCAGTGTC CTGGATTCAC TCATGAGCGC AACATACTTC
    AATGACAACA TCCTTACCCT GATACGGACC CTGGTGACTG GAGGAGCCAC ACCAGAGCTG
    GAGGCTTTGA TCGCTGAGGA GAATGCACTC CGCGGGGGCT ACAGCACCCC CCAGACGCTG
    GCCAACAGGG ACCGCTGCCG TGTGGCGCAG TTAGCGCTGC TGGACGGGCC CTTTGCGGAC
    CTGGGGGATG GTGGTTGTTA TGGTGATCTG TTCTGCAAAG CTCTGAAAAC ATACAATATG
    CTTTGTTTTG GAATTTATCG GCTGAGAGAT GCTCACCTCA GCACCCCCAG CCAGTGCACG
    AAGAGGTATG TCATCACCAA CCCCCCGTAC GAGTTTGAGC TCGTGCCGAC GGACCTGATC
    TTCTGCTTAA TGCAGTTTGA CCACAACGCC GGCCAGTCCC GGGCCAGCCT CTCCCATTCC
    TCCCACTCGT CCCAGTCCTC TAGCAAGAAG AGCTCCTCCG TCCACTCCAT CCCATCCACG
    GCAAACCGAC AGAACCGGCC CAAGTCCCGG GAGTCCCGAG ACAAACAGAA CAGAAAAGAA
    ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231563
    Clone ID Related Accession (Same CDS sequence) XM_024986564.1
    Accession Version XM_024986564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGCTAA ACCTGGCAAG
    TTGCCTTTGG TATCAGTCAA TCAGGAAAAA AACAGTGGGA CGCACATTCT AATGATAACC
    GAACTGGTGA ACGATACTAA TGTTCAGTTT TTGGACCAAG ACGATGACGA TGACCCCGAC
    ACAGAACTGT ACCTCACGCA GCCGTTTGCA TGTGGGACAG CGTTCGCCGT CAGTGTCCTG
    GATTCACTCA TGAGCGCAAC ATACTTCAAT GACAACATCC TTACCCTGAT ACGGACCCTG
    GTGACTGGAG GAGCCACACC AGAGCTGGAG GCTTTGATCG CTGAGGAGAA TGCACTCCGC
    GGGGGCTACA GCACCCCCCA GACGCTGGCC AACAGGGACC GCTGCCGTGT GGCGCAGTTA
    GCGCTGCTGG ACGGGCCCTT TGCGGACCTG GGGGATGGTG GTTGTTATGG TGATCTGTTC
    TGCAAAGCTC TGAAAACATA CAATATGCTT TGTTTTGGAA TTTATCGGCT GAGAGATGCT
    CACCTCAGCA CCCCCAGCCA GTGCACGAAG AGGTATGTCA TCACCAACCC CCCGTACGAG
    TTTGAGCTCG TGCCGACGGA CCTGATCTTC TGCTTAATGC AGTTTGACCA CAACGCCGGC
    CAGTCCCGGG CCAGCCTCTC CCATTCCTCC CACTCGTCCC AGTCCTCTAG CAAGAAGAGC
    TCCTCCGTCC ACTCCATCCC ATCCACGGCA AACCGACAGA ACCGGCCCAA GTCCCGGGAG
    TCCCGAGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGAAAA GAAATGGTTT
    ACAGATGAAC CGGATAATGC CTATCCCAGA AACATTCAAA TCAAGCCCAT GAGTACCCAC
    ATGGCTAACC AGATCAACCA ATATAAATCC ACAAGCAGCT TGATTCCACC AATCAGAGAA
    GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842332.1
    CDS170..3907
    Translation

    Target ORF information:

    RefSeq Version XM_024986564.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGCTAA ACCTGGCAAG
    TTGCCTTTGG TATCAGTCAA TCAGGAAAAA AACAGTGGGA CGCACATTCT AATGATAACC
    GAACTGGTGA ACGATACTAA TGTTCAGTTT TTGGACCAAG ACGATGACGA TGACCCCGAC
    ACAGAACTGT ACCTCACGCA GCCGTTTGCA TGTGGGACAG CGTTCGCCGT CAGTGTCCTG
    GATTCACTCA TGAGCGCAAC ATACTTCAAT GACAACATCC TTACCCTGAT ACGGACCCTG
    GTGACTGGAG GAGCCACACC AGAGCTGGAG GCTTTGATCG CTGAGGAGAA TGCACTCCGC
    GGGGGCTACA GCACCCCCCA GACGCTGGCC AACAGGGACC GCTGCCGTGT GGCGCAGTTA
    GCGCTGCTGG ACGGGCCCTT TGCGGACCTG GGGGATGGTG GTTGTTATGG TGATCTGTTC
    TGCAAAGCTC TGAAAACATA CAATATGCTT TGTTTTGGAA TTTATCGGCT GAGAGATGCT
    CACCTCAGCA CCCCCAGCCA GTGCACGAAG AGGTATGTCA TCACCAACCC CCCGTACGAG
    TTTGAGCTCG TGCCGACGGA CCTGATCTTC TGCTTAATGC AGTTTGACCA CAACGCCGGC
    CAGTCCCGGG CCAGCCTCTC CCATTCCTCC CACTCGTCCC AGTCCTCTAG CAAGAAGAGC
    TCCTCCGTCC ACTCCATCCC ATCCACGGCA AACCGACAGA ACCGGCCCAA GTCCCGGGAG
    TCCCGAGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGAAAA GAAATGGTTT
    ACAGATGAAC CGGATAATGC CTATCCCAGA AACATTCAAA TCAAGCCCAT GAGTACCCAC
    ATGGCTAACC AGATCAACCA ATATAAATCC ACAAGCAGCT TGATTCCACC AATCAGAGAA
    GTTGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231564
    Clone ID Related Accession (Same CDS sequence) XM_024986566.1
    Accession Version XM_024986566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTG ATATATTCCA AGATGTCCAT CTACAAGAGA
    ATGAGACGGG CATGTTGTTT TGATTGCGGA CGTTCTGAGC GTGACTGCTC GTGCATGTCA
    GGCCGTGTGC GTGGTAACGT GGACACCCTT GAGAGAGCCT TCCCACTTTC TTCTGTCTCT
    GTTAGTGATT GCTCCACCAC TTTCCGTGCC TTTGAAGATG AACAGCCGTC GACACTGTCA
    CCCAAAAAAA AGCAGCGCAA CGGAGGCATG CGGAACTCAC CCAGCTCGTC GCCCAAGCTG
    ATGAGGCATG ACCCCTTGTT AATTCCTGGC AATGATCAGA TTGACAACAT GGACTCCAAT
    GTGAAGAAAT ATGACTCTAC TGGGATGTTT CACTGGTGTG CGCCCAAGGA GATAGAGAAA
    GTCATCCTGA CCCGAAGTGA AGCTGCCATG ACCGTCCTGA GCGGCCACGT GGTGGTCTGC
    ATCTTTGGCG ACGTCAGCTC TGCCCTGATT GGCCTTCGGA ACCTGGTGAT GCCGCTCCGT
    GCCAGCAACT TTCACTACCA CGAGCTCAAG CACATCGTGT TTGTGGGCTC CATCGAGTAC
    CTCAAGCGGG AATGGGAGAC ACTGCATAAC TTCCCCAAGG TTTCCATCTT GCCTGGTACG
    CCATTAAGTC GGGCTGATTT AAGGGCCGTC AACATCAACC TCTGTGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACTTCGCTGC AGGACAAGGA ATGCATCTTG
    GCGTCACTCA ACATCAAATC TATGCAGTTT GATGACAGCA TCGGGGTCTT GCAGGCTAAT
    TCCCAAGGGT TCACACCTCC AGGAATGGAC CGATCCTCAC CAGATAACAG CCCAGTACAT
    GGGATGTTAC GCCAGCCCTC CATCACGACT GGGGTCAACA TCCCCATCAT CACTGAACTA
    GTGAACGATA CTAATGTTCA GTTTTTGGAC CAAGACGATG ACGATGACCC CGACACAGAA
    CTGTACCTCA CGCAGCCGTT TGCATGTGGG ACAGCGTTCG CCGTCAGTGT CCTGGATTCA
    CTCATGAGCG CAACATACTT CAATGACAAC ATCCTTACCC TGATACGGAC CCTGGTGACT
    GGAGGAGCCA CACCAGAGCT GGAGGCTTTG ATCGCTGAGG AGAATGCACT CCGCGGGGGC
    TACAGCACCC CCCAGACGCT GGCCAACAGG GACCGCTGCC GTGTGGCGCA GTTAGCGCTG
    CTGGACGGGC CCTTTGCGGA CCTGGGGGAT GGTGGTTGTT ATGGTGATCT GTTCTGCAAA
    GCTCTGAAAA CATACAATAT GCTTTGTTTT GGAATTTATC GGCTGAGAGA TGCTCACCTC
    AGCACCCCCA GCCAGTGCAC GAAGAGGTAT GTCATCACCA ACCCCCCGTA CGAGTTTGAG
    CTCGTGCCGA CGGACCTGAT CTTCTGCTTA ATGCAGTTTG ACCACAACGC CGGCCAGTCC
    CGGGCCAGCC TCTCCCATTC CTCCCACTCG TCCCAGTCCT CTAGCAAGAA GAGCTCCTCC
    GTCCACTCCA TCCCATCCAC GGCAAACCGA CAGAACCGGC CCAAGTCCCG GGAGTCCCGA
    GACAAACAGA AAAAAGAAAT GGTTTACAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842334.1
    CDS169..3861
    Translation

    Target ORF information:

    RefSeq Version XM_024986566.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTG ATATATTCCA AGATGTCCAT CTACAAGAGA
    ATGAGACGGG CATGTTGTTT TGATTGCGGA CGTTCTGAGC GTGACTGCTC GTGCATGTCA
    GGCCGTGTGC GTGGTAACGT GGACACCCTT GAGAGAGCCT TCCCACTTTC TTCTGTCTCT
    GTTAGTGATT GCTCCACCAC TTTCCGTGCC TTTGAAGATG AACAGCCGTC GACACTGTCA
    CCCAAAAAAA AGCAGCGCAA CGGAGGCATG CGGAACTCAC CCAGCTCGTC GCCCAAGCTG
    ATGAGGCATG ACCCCTTGTT AATTCCTGGC AATGATCAGA TTGACAACAT GGACTCCAAT
    GTGAAGAAAT ATGACTCTAC TGGGATGTTT CACTGGTGTG CGCCCAAGGA GATAGAGAAA
    GTCATCCTGA CCCGAAGTGA AGCTGCCATG ACCGTCCTGA GCGGCCACGT GGTGGTCTGC
    ATCTTTGGCG ACGTCAGCTC TGCCCTGATT GGCCTTCGGA ACCTGGTGAT GCCGCTCCGT
    GCCAGCAACT TTCACTACCA CGAGCTCAAG CACATCGTGT TTGTGGGCTC CATCGAGTAC
    CTCAAGCGGG AATGGGAGAC ACTGCATAAC TTCCCCAAGG TTTCCATCTT GCCTGGTACG
    CCATTAAGTC GGGCTGATTT AAGGGCCGTC AACATCAACC TCTGTGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACTTCGCTGC AGGACAAGGA ATGCATCTTG
    GCGTCACTCA ACATCAAATC TATGCAGTTT GATGACAGCA TCGGGGTCTT GCAGGCTAAT
    TCCCAAGGGT TCACACCTCC AGGAATGGAC CGATCCTCAC CAGATAACAG CCCAGTACAT
    GGGATGTTAC GCCAGCCCTC CATCACGACT GGGGTCAACA TCCCCATCAT CACTGAACTA
    GTGAACGATA CTAATGTTCA GTTTTTGGAC CAAGACGATG ACGATGACCC CGACACAGAA
    CTGTACCTCA CGCAGCCGTT TGCATGTGGG ACAGCGTTCG CCGTCAGTGT CCTGGATTCA
    CTCATGAGCG CAACATACTT CAATGACAAC ATCCTTACCC TGATACGGAC CCTGGTGACT
    GGAGGAGCCA CACCAGAGCT GGAGGCTTTG ATCGCTGAGG AGAATGCACT CCGCGGGGGC
    TACAGCACCC CCCAGACGCT GGCCAACAGG GACCGCTGCC GTGTGGCGCA GTTAGCGCTG
    CTGGACGGGC CCTTTGCGGA CCTGGGGGAT GGTGGTTGTT ATGGTGATCT GTTCTGCAAA
    GCTCTGAAAA CATACAATAT GCTTTGTTTT GGAATTTATC GGCTGAGAGA TGCTCACCTC
    AGCACCCCCA GCCAGTGCAC GAAGAGGTAT GTCATCACCA ACCCCCCGTA CGAGTTTGAG
    CTCGTGCCGA CGGACCTGAT CTTCTGCTTA ATGCAGTTTG ACCACAACGC CGGCCAGTCC
    CGGGCCAGCC TCTCCCATTC CTCCCACTCG TCCCAGTCCT CTAGCAAGAA GAGCTCCTCC
    GTCCACTCCA TCCCATCCAC GGCAAACCGA CAGAACCGGC CCAAGTCCCG GGAGTCCCGA
    GACAAACAGA AAAAAGAAAT GGTTTACAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231565
    Clone ID Related Accession (Same CDS sequence) XM_024986567.1
    Accession Version XM_024986567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAATGCA
    ACAAGGATGA ATAGAATGGG CCAAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGTACCCACA TGGCTAACCA GATCAACCAA
    TATAAATCCA CAAGCAGCTT GATTCCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842335.1
    CDS169..3825
    Translation

    Target ORF information:

    RefSeq Version XM_024986567.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAATGCA
    ACAAGGATGA ATAGAATGGG CCAAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGTACCCACA TGGCTAACCA GATCAACCAA
    TATAAATCCA CAAGCAGCTT GATTCCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231566
    Clone ID Related Accession (Same CDS sequence) XM_024986568.1
    Accession Version XM_024986568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTG ATATATTCCA AGATGTCCAT CTACAAGAGA
    ATGAGACGGG CATGTTGTTT TGATTGCGGA CGTTCTGAGC GTGACTGCTC GTGCATGTCA
    GGCCGTGTGC GTGGTAACGT GGACACCCTT GAGAGAGCCT TCCCACTTTC TTCTGTCTCT
    GTTAGTGATT GCTCCACCAC TTTCCGTGCC TTTGAAGATG AACAGCCGTC GACACTGTCA
    CCCAAAAAAA AGCAGCGCAA CGGAGGCATG CGGAACTCAC CCAGCTCGTC GCCCAAGCTG
    ATGAGGCATG ACCCCTTGTT AATTCCTGGC AATGATCAGA TTGACAACAT GGACTCCAAT
    GTGAAGAAAT ATGACTCTAC TGGGATGTTT CACTGGTGTG CGCCCAAGGA GATAGAGAAA
    GTCATCCTGA CCCGAAGTGA AGCTGCCATG ACCGTCCTGA GCGGCCACGT GGTGGTCTGC
    ATCTTTGGCG ACGTCAGCTC TGCCCTGATT GGCCTTCGGA ACCTGGTGAT GCCGCTCCGT
    GCCAGCAACT TTCACTACCA CGAGCTCAAG CACATCGTGT TTGTGGGCTC CATCGAGTAC
    CTCAAGCGGG AATGGGAGAC ACTGCATAAC TTCCCCAAGG TTTCCATCTT GCCTGGTACG
    CCATTAAGTC GGGCTGATTT AAGGGCCGTC AACATCAACC TCTGTGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACTTCGCTGC AGGACAAGGA ATGCATCTTG
    GCGTCACTCA ACATCAAATC TATGCAGTTT GATGACAGCA TCGGGGTCTT GCAGGCTAAT
    TCCCAAGGGT TCACACCTCC AGGAATGGAC CGATCCTCAC CAGATAACAG CCCAGTACAT
    GGGATGTTAC GCCAGCCCTC CATCACGACT GGGGTCAACA TCCCCATCAT CACTGAACTA
    GTGAACGATA CTAATGTTCA GTTTTTGGAC CAAGACGATG ACGATGACCC CGACACAGAA
    CTGTACCTCA CGCAGCCGTT TGCATGTGGG ACAGCGTTCG CCGTCAGTGT CCTGGATTCA
    CTCATGAGCG CAACATACTT CAATGACAAC ATCCTTACCC TGATACGGAC CCTGGTGACT
    GGAGGAGCCA CACCAGAGCT GGAGGCTTTG ATCGCTGAGG AGAATGCACT CCGCGGGGGC
    TACAGCACCC CCCAGACGCT GGCCAACAGG GACCGCTGCC GTGTGGCGCA GTTAGCGCTG
    CTGGACGGGC CCTTTGCGGA CCTGGGGGAT GGTGGTTGTT ATGGTGATCT GTTCTGCAAA
    GCTCTGAAAA CATACAATAT GCTTTGTTTT GGAATTTATC GGCTGAGAGA TGCTCACCTC
    AGCACCCCCA GCCAGTGCAC GAAGAGGTAT GTCATCACCA ACCCCCCGTA CGAGTTTGAG
    CTCGTGCCGA CGGACCTGAT CTTCTGCTTA ATGCAGTTTG ACCACAACGC CGGCCAGTCC
    CGGGCCAGCC TCTCCCATTC CTCCCACTCG TCCCAGTCCT CTAGCAAGAA GAGCTCCTCC
    GTCCACTCCA TCCCATCCAC GGCAAACCGA CAGAACCGGC CCAAGTCCCG GGAGTCCCGA
    GACAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCAAG AAAAGAAATG GTTTACAGAT
    GAACCGGATA ATGCCTATCC CAGAAACATT CAAATCAAGC CCATGAGTAC CCACATGGCT
    AACCAGATCA ACCAATATAA ATCCACAAGC AGCTTGATTC CACCAATCAG AGAAGTTGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842336.1
    CDS170..4021
    Translation

    Target ORF information:

    RefSeq Version XM_024986568.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTG ATATATTCCA AGATGTCCAT CTACAAGAGA
    ATGAGACGGG CATGTTGTTT TGATTGCGGA CGTTCTGAGC GTGACTGCTC GTGCATGTCA
    GGCCGTGTGC GTGGTAACGT GGACACCCTT GAGAGAGCCT TCCCACTTTC TTCTGTCTCT
    GTTAGTGATT GCTCCACCAC TTTCCGTGCC TTTGAAGATG AACAGCCGTC GACACTGTCA
    CCCAAAAAAA AGCAGCGCAA CGGAGGCATG CGGAACTCAC CCAGCTCGTC GCCCAAGCTG
    ATGAGGCATG ACCCCTTGTT AATTCCTGGC AATGATCAGA TTGACAACAT GGACTCCAAT
    GTGAAGAAAT ATGACTCTAC TGGGATGTTT CACTGGTGTG CGCCCAAGGA GATAGAGAAA
    GTCATCCTGA CCCGAAGTGA AGCTGCCATG ACCGTCCTGA GCGGCCACGT GGTGGTCTGC
    ATCTTTGGCG ACGTCAGCTC TGCCCTGATT GGCCTTCGGA ACCTGGTGAT GCCGCTCCGT
    GCCAGCAACT TTCACTACCA CGAGCTCAAG CACATCGTGT TTGTGGGCTC CATCGAGTAC
    CTCAAGCGGG AATGGGAGAC ACTGCATAAC TTCCCCAAGG TTTCCATCTT GCCTGGTACG
    CCATTAAGTC GGGCTGATTT AAGGGCCGTC AACATCAACC TCTGTGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACTTCGCTGC AGGACAAGGA ATGCATCTTG
    GCGTCACTCA ACATCAAATC TATGCAGTTT GATGACAGCA TCGGGGTCTT GCAGGCTAAT
    TCCCAAGGGT TCACACCTCC AGGAATGGAC CGATCCTCAC CAGATAACAG CCCAGTACAT
    GGGATGTTAC GCCAGCCCTC CATCACGACT GGGGTCAACA TCCCCATCAT CACTGAACTA
    GTGAACGATA CTAATGTTCA GTTTTTGGAC CAAGACGATG ACGATGACCC CGACACAGAA
    CTGTACCTCA CGCAGCCGTT TGCATGTGGG ACAGCGTTCG CCGTCAGTGT CCTGGATTCA
    CTCATGAGCG CAACATACTT CAATGACAAC ATCCTTACCC TGATACGGAC CCTGGTGACT
    GGAGGAGCCA CACCAGAGCT GGAGGCTTTG ATCGCTGAGG AGAATGCACT CCGCGGGGGC
    TACAGCACCC CCCAGACGCT GGCCAACAGG GACCGCTGCC GTGTGGCGCA GTTAGCGCTG
    CTGGACGGGC CCTTTGCGGA CCTGGGGGAT GGTGGTTGTT ATGGTGATCT GTTCTGCAAA
    GCTCTGAAAA CATACAATAT GCTTTGTTTT GGAATTTATC GGCTGAGAGA TGCTCACCTC
    AGCACCCCCA GCCAGTGCAC GAAGAGGTAT GTCATCACCA ACCCCCCGTA CGAGTTTGAG
    CTCGTGCCGA CGGACCTGAT CTTCTGCTTA ATGCAGTTTG ACCACAACGC CGGCCAGTCC
    CGGGCCAGCC TCTCCCATTC CTCCCACTCG TCCCAGTCCT CTAGCAAGAA GAGCTCCTCC
    GTCCACTCCA TCCCATCCAC GGCAAACCGA CAGAACCGGC CCAAGTCCCG GGAGTCCCGA
    GACAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCAAG AAAAGAAATG GTTTACAGAT
    GAACCGGATA ATGCCTATCC CAGAAACATT CAAATCAAGC CCATGAGTAC CCACATGGCT
    AACCAGATCA ACCAATATAA ATCCACAAGC AGCTTGATTC CACCAATCAG AGAAGTTGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231567
    Clone ID Related Accession (Same CDS sequence) XM_024986569.1
    Accession Version XM_024986569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTGAT ATATTTTGAA GATGAACAGC CGTCGACACT GTCACCCAAA
    AAAAAGCAGC GCAACGGAGG CATGCGGAAC TCACCCAGCT CGTCGCCCAA GCTGATGAGG
    CATGACCCCT TGTTAATTCC TGGCAATGAT CAGATTGACA ACATGGACTC CAATGTGAAG
    AAATATGACT CTACTGGGAT GTTTCACTGG TGTGCGCCCA AGGAGATAGA GAAAGTCATC
    CTGACCCGAA GTGAAGCTGC CATGACCGTC CTGAGCGGCC ACGTGGTGGT CTGCATCTTT
    GGCGACGTCA GCTCTGCCCT GATTGGCCTT CGGAACCTGG TGATGCCGCT CCGTGCCAGC
    AACTTTCACT ACCACGAGCT CAAGCACATC GTGTTTGTGG GCTCCATCGA GTACCTCAAG
    CGGGAATGGG AGACACTGCA TAACTTCCCC AAGGTTTCCA TCTTGCCTGG TACGCCATTA
    AGTCGGGCTG ATTTAAGGGC CGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA
    CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA
    GGGTTCACAC CTCCAGGAAT GGACCGATCC TCACCAGATA ACAGCCCAGT ACATGGGATG
    TTACGCCAGC CCTCCATCAC GACTGGGGTC AACATCCCCA TCATCACTGA ACTAGCTAAA
    CCTGGCAAGT TGCCTTTGGT ATCAGTCAAT CAGGAAAAAA ACAGTGGGAC GCACATTCTA
    ATGATAACCG AACTGGTGAA CGATACTAAT GTTCAGTTTT TGGACCAAGA CGATGACGAT
    GACCCCGACA CAGAACTGTA CCTCACGCAG CCGTTTGCAT GTGGGACAGC GTTCGCCGTC
    AGTGTCCTGG ATTCACTCAT GAGCGCAACA TACTTCAATG ACAACATCCT TACCCTGATA
    CGGACCCTGG TGACTGGAGG AGCCACACCA GAGCTGGAGG CTTTGATCGC TGAGGAGAAT
    GCACTCCGCG GGGGCTACAG CACCCCCCAG ACGCTGGCCA ACAGGGACCG CTGCCGTGTG
    GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT GCGGACCTGG GGGATGGTGG TTGTTATGGT
    GATCTGTTCT GCAAAGCTCT GAAAACATAC AATATGCTTT GTTTTGGAAT TTATCGGCTG
    AGAGATGCTC ACCTCAGCAC CCCCAGCCAG TGCACGAAGA GGTATGTCAT CACCAACCCC
    CCGTACGAGT TTGAGCTCGT GCCGACGGAC CTGATCTTCT GCTTAATGCA GTTTGACCAC
    AACGCCGGCC AGTCCCGGGC CAGCCTCTCC CATTCCTCCC ACTCGTCCCA GTCCTCTAGC
    AAGAAGAGCT CCTCCGTCCA CTCCATCCCA TCCACGGCAA ACCGACAGAA CCGGCCCAAG
    TCCCGGGAGT CCCGAGACAA ACAGAAGTAC GTGCAGGAAG AGCGGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842337.1
    CDS169..3759
    Translation

    Target ORF information:

    RefSeq Version XM_024986569.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTGAT ATATTTTGAA GATGAACAGC CGTCGACACT GTCACCCAAA
    AAAAAGCAGC GCAACGGAGG CATGCGGAAC TCACCCAGCT CGTCGCCCAA GCTGATGAGG
    CATGACCCCT TGTTAATTCC TGGCAATGAT CAGATTGACA ACATGGACTC CAATGTGAAG
    AAATATGACT CTACTGGGAT GTTTCACTGG TGTGCGCCCA AGGAGATAGA GAAAGTCATC
    CTGACCCGAA GTGAAGCTGC CATGACCGTC CTGAGCGGCC ACGTGGTGGT CTGCATCTTT
    GGCGACGTCA GCTCTGCCCT GATTGGCCTT CGGAACCTGG TGATGCCGCT CCGTGCCAGC
    AACTTTCACT ACCACGAGCT CAAGCACATC GTGTTTGTGG GCTCCATCGA GTACCTCAAG
    CGGGAATGGG AGACACTGCA TAACTTCCCC AAGGTTTCCA TCTTGCCTGG TACGCCATTA
    AGTCGGGCTG ATTTAAGGGC CGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA
    CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA
    GGGTTCACAC CTCCAGGAAT GGACCGATCC TCACCAGATA ACAGCCCAGT ACATGGGATG
    TTACGCCAGC CCTCCATCAC GACTGGGGTC AACATCCCCA TCATCACTGA ACTAGCTAAA
    CCTGGCAAGT TGCCTTTGGT ATCAGTCAAT CAGGAAAAAA ACAGTGGGAC GCACATTCTA
    ATGATAACCG AACTGGTGAA CGATACTAAT GTTCAGTTTT TGGACCAAGA CGATGACGAT
    GACCCCGACA CAGAACTGTA CCTCACGCAG CCGTTTGCAT GTGGGACAGC GTTCGCCGTC
    AGTGTCCTGG ATTCACTCAT GAGCGCAACA TACTTCAATG ACAACATCCT TACCCTGATA
    CGGACCCTGG TGACTGGAGG AGCCACACCA GAGCTGGAGG CTTTGATCGC TGAGGAGAAT
    GCACTCCGCG GGGGCTACAG CACCCCCCAG ACGCTGGCCA ACAGGGACCG CTGCCGTGTG
    GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT GCGGACCTGG GGGATGGTGG TTGTTATGGT
    GATCTGTTCT GCAAAGCTCT GAAAACATAC AATATGCTTT GTTTTGGAAT TTATCGGCTG
    AGAGATGCTC ACCTCAGCAC CCCCAGCCAG TGCACGAAGA GGTATGTCAT CACCAACCCC
    CCGTACGAGT TTGAGCTCGT GCCGACGGAC CTGATCTTCT GCTTAATGCA GTTTGACCAC
    AACGCCGGCC AGTCCCGGGC CAGCCTCTCC CATTCCTCCC ACTCGTCCCA GTCCTCTAGC
    AAGAAGAGCT CCTCCGTCCA CTCCATCCCA TCCACGGCAA ACCGACAGAA CCGGCCCAAG
    TCCCGGGAGT CCCGAGACAA ACAGAAGTAC GTGCAGGAAG AGCGGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231568
    Clone ID Related Accession (Same CDS sequence) XM_024986570.1
    Accession Version XM_024986570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTT GAAGATGAAC AGCCGTCGAC ACTGTCACCC
    AAAAAAAAGC AGCGCAACGG AGGCATGCGG AACTCACCCA GCTCGTCGCC CAAGCTGATG
    AGGCATGACC CCTTGTTAAT TCCTGGCAAT GATCAGATTG ACAACATGGA CTCCAATGTG
    AAGAAATATG ACTCTACTGG GATGTTTCAC TGGTGTGCGC CCAAGGAGAT AGAGAAAGTC
    ATCCTGACCC GAAGTGAAGC TGCCATGACC GTCCTGAGCG GCCACGTGGT GGTCTGCATC
    TTTGGCGACG TCAGCTCTGC CCTGATTGGC CTTCGGAACC TGGTGATGCC GCTCCGTGCC
    AGCAACTTTC ACTACCACGA GCTCAAGCAC ATCGTGTTTG TGGGCTCCAT CGAGTACCTC
    AAGCGGGAAT GGGAGACACT GCATAACTTC CCCAAGGTTT CCATCTTGCC TGGTACGCCA
    TTAAGTCGGG CTGATTTAAG GGCCGTCAAC ATCAACCTCT GTGACATGTG CGTTATCCTG
    TCAGCCAATC AGAATAATAT TGATGATACT TCGCTGCAGG ACAAGGAATG CATCTTGGCG
    TCACTCAACA TCAAATCTAT GCAGTTTGAT GACAGCATCG GGGTCTTGCA GGCTAATTCC
    CAAGGGTTCA CACCTCCAGG AATGGACCGA TCCTCACCAG ATAACAGCCC AGTACATGGG
    ATGTTACGCC AGCCCTCCAT CACGACTGGG GTCAACATCC CCATCATCAC TGAACTAGCT
    AAACCTGGCA AGTTGCCTTT GGTATCAGTC AATCAGGAAA AAAACAGTGG GACGCACATT
    CTAATGATAA CCGAACTGGT GAACGATACT AATGTTCAGT TTTTGGACCA AGACGATGAC
    GATGACCCCG ACACAGAACT GTACCTCACG CAGCCGTTTG CATGTGGGAC AGCGTTCGCC
    GTCAGTGTCC TGGATTCACT CATGAGCGCA ACATACTTCA ATGACAACAT CCTTACCCTG
    ATACGGACCC TGGTGACTGG AGGAGCCACA CCAGAGCTGG AGGCTTTGAT CGCTGAGGAG
    AATGCACTCC GCGGGGGCTA CAGCACCCCC CAGACGCTGG CCAACAGGGA CCGCTGCCGT
    GTGGCGCAGT TAGCGCTGCT GGACGGGCCC TTTGCGGACC TGGGGGATGG TGGTTGTTAT
    GGTGATCTGT TCTGCAAAGC TCTGAAAACA TACAATATGC TTTGTTTTGG AATTTATCGG
    CTGAGAGATG CTCACCTCAG CACCCCCAGC CAGTGCACGA AGAGGTATGT CATCACCAAC
    CCCCCGTACG AGTTTGAGCT CGTGCCGACG GACCTGATCT TCTGCTTAAT GCAGTTTGAC
    CACAACGCCG GCCAGTCCCG GGCCAGCCTC TCCCATTCCT CCCACTCGTC CCAGTCCTCT
    AGCAAGAAGA GCTCCTCCGT CCACTCCATC CCATCCACGG CAAACCGACA GAACCGGCCC
    AAGTCCCGGG AGTCCCGAGA CAAACAGAAA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842338.1
    CDS169..3759
    Translation

    Target ORF information:

    RefSeq Version XM_024986570.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTT GAAGATGAAC AGCCGTCGAC ACTGTCACCC
    AAAAAAAAGC AGCGCAACGG AGGCATGCGG AACTCACCCA GCTCGTCGCC CAAGCTGATG
    AGGCATGACC CCTTGTTAAT TCCTGGCAAT GATCAGATTG ACAACATGGA CTCCAATGTG
    AAGAAATATG ACTCTACTGG GATGTTTCAC TGGTGTGCGC CCAAGGAGAT AGAGAAAGTC
    ATCCTGACCC GAAGTGAAGC TGCCATGACC GTCCTGAGCG GCCACGTGGT GGTCTGCATC
    TTTGGCGACG TCAGCTCTGC CCTGATTGGC CTTCGGAACC TGGTGATGCC GCTCCGTGCC
    AGCAACTTTC ACTACCACGA GCTCAAGCAC ATCGTGTTTG TGGGCTCCAT CGAGTACCTC
    AAGCGGGAAT GGGAGACACT GCATAACTTC CCCAAGGTTT CCATCTTGCC TGGTACGCCA
    TTAAGTCGGG CTGATTTAAG GGCCGTCAAC ATCAACCTCT GTGACATGTG CGTTATCCTG
    TCAGCCAATC AGAATAATAT TGATGATACT TCGCTGCAGG ACAAGGAATG CATCTTGGCG
    TCACTCAACA TCAAATCTAT GCAGTTTGAT GACAGCATCG GGGTCTTGCA GGCTAATTCC
    CAAGGGTTCA CACCTCCAGG AATGGACCGA TCCTCACCAG ATAACAGCCC AGTACATGGG
    ATGTTACGCC AGCCCTCCAT CACGACTGGG GTCAACATCC CCATCATCAC TGAACTAGCT
    AAACCTGGCA AGTTGCCTTT GGTATCAGTC AATCAGGAAA AAAACAGTGG GACGCACATT
    CTAATGATAA CCGAACTGGT GAACGATACT AATGTTCAGT TTTTGGACCA AGACGATGAC
    GATGACCCCG ACACAGAACT GTACCTCACG CAGCCGTTTG CATGTGGGAC AGCGTTCGCC
    GTCAGTGTCC TGGATTCACT CATGAGCGCA ACATACTTCA ATGACAACAT CCTTACCCTG
    ATACGGACCC TGGTGACTGG AGGAGCCACA CCAGAGCTGG AGGCTTTGAT CGCTGAGGAG
    AATGCACTCC GCGGGGGCTA CAGCACCCCC CAGACGCTGG CCAACAGGGA CCGCTGCCGT
    GTGGCGCAGT TAGCGCTGCT GGACGGGCCC TTTGCGGACC TGGGGGATGG TGGTTGTTAT
    GGTGATCTGT TCTGCAAAGC TCTGAAAACA TACAATATGC TTTGTTTTGG AATTTATCGG
    CTGAGAGATG CTCACCTCAG CACCCCCAGC CAGTGCACGA AGAGGTATGT CATCACCAAC
    CCCCCGTACG AGTTTGAGCT CGTGCCGACG GACCTGATCT TCTGCTTAAT GCAGTTTGAC
    CACAACGCCG GCCAGTCCCG GGCCAGCCTC TCCCATTCCT CCCACTCGTC CCAGTCCTCT
    AGCAAGAAGA GCTCCTCCGT CCACTCCATC CCATCCACGG CAAACCGACA GAACCGGCCC
    AAGTCCCGGG AGTCCCGAGA CAAACAGAAA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231569
    Clone ID Related Accession (Same CDS sequence) XM_024986571.1
    Accession Version XM_024986571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTT GAAGATGAAC AGCCGTCGAC ACTGTCACCC
    AAAAAAAAGC AGCGCAACGG AGGCATGCGG AACTCACCCA GCTCGTCGCC CAAGCTGATG
    AGGCATGACC CCTTGTTAAT TCCTGGCAAT GATCAGATTG ACAACATGGA CTCCAATGTG
    AAGAAATATG ACTCTACTGG GATGTTTCAC TGGTGTGCGC CCAAGGAGAT AGAGAAAGTC
    ATCCTGACCC GAAGTGAAGC TGCCATGACC GTCCTGAGCG GCCACGTGGT GGTCTGCATC
    TTTGGCGACG TCAGCTCTGC CCTGATTGGC CTTCGGAACC TGGTGATGCC GCTCCGTGCC
    AGCAACTTTC ACTACCACGA GCTCAAGCAC ATCGTGTTTG TGGGCTCCAT CGAGTACCTC
    AAGCGGGAAT GGGAGACACT GCATAACTTC CCCAAGGTTT CCATCTTGCC TGGTACGCCA
    TTAAGTCGGG CTGATTTAAG GGCCGTCAAC ATCAACCTCT GTGACATGTG CGTTATCCTG
    TCAGCCAATC AGAATAATAT TGATGATACT TCGCTGCAGG ACAAGGAATG CATCTTGGCG
    TCACTCAACA TCAAATCTAT GCAGTTTGAT GACAGCATCG GGGTCTTGCA GGCTAATTCC
    CAAGGGTTCA CACCTCCAGG AATGGACCGA TCCTCACCAG ATAACAGCCC AGTACATGGG
    ATGTTACGCC AGCCCTCCAT CACGACTGGG GTCAACATCC CCATCATCAC TGAACTAGCT
    AAACCTGGCA AGTTGCCTTT GGTATCAGTC AATCAGGAAA AAAACAGTGG GACGCACATT
    CTAATGATAA CCGAACTGGT GAACGATACT AATGTTCAGT TTTTGGACCA AGACGATGAC
    GATGACCCCG ACACAGAACT GTACCTCACG CAGCCGTTTG CATGTGGGAC AGCGTTCGCC
    GTCAGTGTCC TGGATTCACT CATGAGCGCA ACATACTTCA ATGACAACAT CCTTACCCTG
    ATACGGACCC TGGTGACTGG AGGAGCCACA CCAGAGCTGG AGGCTTTGAT CGCTGAGGAG
    AATGCACTCC GCGGGGGCTA CAGCACCCCC CAGACGCTGG CCAACAGGGA CCGCTGCCGT
    GTGGCGCAGT TAGCGCTGCT GGACGGGCCC TTTGCGGACC TGGGGGATGG TGGTTGTTAT
    GGTGATCTGT TCTGCAAAGC TCTGAAAACA TACAATATGC TTTGTTTTGG AATTTATCGG
    CTGAGAGATG CTCACCTCAG CACCCCCAGC CAGTGCACGA AGAGGTATGT CATCACCAAC
    CCCCCGTACG AGTTTGAGCT CGTGCCGACG GACCTGATCT TCTGCTTAAT GCAGTTTGAC
    CACAACGCCG GCCAGTCCCG GGCCAGCCTC TCCCATTCCT CCCACTCGTC CCAGTCCTCT
    AGCAAGAAGA GCTCCTCCGT CCACTCCATC CCATCCACGG CAAACCGACA GAACCGGCCC
    AAGTCCCGGG AGTCCCGAGA CAAACAGAAC AGAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842339.1
    CDS169..3762
    Translation

    Target ORF information:

    RefSeq Version XM_024986571.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGAAGCCGAA AGCGTATATT AATTAATCCT
    GGAAACCATC TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA
    GAAGTTAAAA GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA
    AGGATAAAAA AATGCGGCTG CAAACGGCTT GAAGATGAAC AGCCGTCGAC ACTGTCACCC
    AAAAAAAAGC AGCGCAACGG AGGCATGCGG AACTCACCCA GCTCGTCGCC CAAGCTGATG
    AGGCATGACC CCTTGTTAAT TCCTGGCAAT GATCAGATTG ACAACATGGA CTCCAATGTG
    AAGAAATATG ACTCTACTGG GATGTTTCAC TGGTGTGCGC CCAAGGAGAT AGAGAAAGTC
    ATCCTGACCC GAAGTGAAGC TGCCATGACC GTCCTGAGCG GCCACGTGGT GGTCTGCATC
    TTTGGCGACG TCAGCTCTGC CCTGATTGGC CTTCGGAACC TGGTGATGCC GCTCCGTGCC
    AGCAACTTTC ACTACCACGA GCTCAAGCAC ATCGTGTTTG TGGGCTCCAT CGAGTACCTC
    AAGCGGGAAT GGGAGACACT GCATAACTTC CCCAAGGTTT CCATCTTGCC TGGTACGCCA
    TTAAGTCGGG CTGATTTAAG GGCCGTCAAC ATCAACCTCT GTGACATGTG CGTTATCCTG
    TCAGCCAATC AGAATAATAT TGATGATACT TCGCTGCAGG ACAAGGAATG CATCTTGGCG
    TCACTCAACA TCAAATCTAT GCAGTTTGAT GACAGCATCG GGGTCTTGCA GGCTAATTCC
    CAAGGGTTCA CACCTCCAGG AATGGACCGA TCCTCACCAG ATAACAGCCC AGTACATGGG
    ATGTTACGCC AGCCCTCCAT CACGACTGGG GTCAACATCC CCATCATCAC TGAACTAGCT
    AAACCTGGCA AGTTGCCTTT GGTATCAGTC AATCAGGAAA AAAACAGTGG GACGCACATT
    CTAATGATAA CCGAACTGGT GAACGATACT AATGTTCAGT TTTTGGACCA AGACGATGAC
    GATGACCCCG ACACAGAACT GTACCTCACG CAGCCGTTTG CATGTGGGAC AGCGTTCGCC
    GTCAGTGTCC TGGATTCACT CATGAGCGCA ACATACTTCA ATGACAACAT CCTTACCCTG
    ATACGGACCC TGGTGACTGG AGGAGCCACA CCAGAGCTGG AGGCTTTGAT CGCTGAGGAG
    AATGCACTCC GCGGGGGCTA CAGCACCCCC CAGACGCTGG CCAACAGGGA CCGCTGCCGT
    GTGGCGCAGT TAGCGCTGCT GGACGGGCCC TTTGCGGACC TGGGGGATGG TGGTTGTTAT
    GGTGATCTGT TCTGCAAAGC TCTGAAAACA TACAATATGC TTTGTTTTGG AATTTATCGG
    CTGAGAGATG CTCACCTCAG CACCCCCAGC CAGTGCACGA AGAGGTATGT CATCACCAAC
    CCCCCGTACG AGTTTGAGCT CGTGCCGACG GACCTGATCT TCTGCTTAAT GCAGTTTGAC
    CACAACGCCG GCCAGTCCCG GGCCAGCCTC TCCCATTCCT CCCACTCGTC CCAGTCCTCT
    AGCAAGAAGA GCTCCTCCGT CCACTCCATC CCATCCACGG CAAACCGACA GAACCGGCCC
    AAGTCCCGGG AGTCCCGAGA CAAACAGAAC AGAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231570
    Clone ID Related Accession (Same CDS sequence) XM_024986572.1
    Accession Version XM_024986572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTGAT ATATTTTGAA GATGAACAGC CGTCGACACT GTCACCCAAA
    AAAAAGCAGC GCAACGGAGG CATGCGGAAC TCACCCAGCT CGTCGCCCAA GCTGATGAGG
    CATGACCCCT TGTTAATTCC TGGCAATGAT CAGATTGACA ACATGGACTC CAATGTGAAG
    AAATATGACT CTACTGGGAT GTTTCACTGG TGTGCGCCCA AGGAGATAGA GAAAGTCATC
    CTGACCCGAA GTGAAGCTGC CATGACCGTC CTGAGCGGCC ACGTGGTGGT CTGCATCTTT
    GGCGACGTCA GCTCTGCCCT GATTGGCCTT CGGAACCTGG TGATGCCGCT CCGTGCCAGC
    AACTTTCACT ACCACGAGCT CAAGCACATC GTGTTTGTGG GCTCCATCGA GTACCTCAAG
    CGGGAATGGG AGACACTGCA TAACTTCCCC AAGGTTTCCA TCTTGCCTGG TACGCCATTA
    AGTCGGGCTG ATTTAAGGGC CGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA
    CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA
    GGGTTCACAC CTCCAGGAAT GGACCGATCC TCACCAGATA ACAGCCCAGT ACATGGGATG
    TTACGCCAGC CCTCCATCAC GACTGGGGTC AACATCCCCA TCATCACTGA ACTAGCTAAA
    CCTGGCAAGT TGCCTTTGGT ATCAGTCAAT CAGGAAAAAA ACAGTGGGAC GCACATTCTA
    ATGATAACCG AACTGGTGAA CGATACTAAT GTTCAGTTTT TGGACCAAGA CGATGACGAT
    GACCCCGACA CAGAACTGTA CCTCACGCAG CCGTTTGCAT GTGGGACAGC GTTCGCCGTC
    AGTGTCCTGG ATTCACTCAT GAGCGCAACA TACTTCAATG ACAACATCCT TACCCTGATA
    CGGACCCTGG TGACTGGAGG AGCCACACCA GAGCTGGAGG CTTTGATCGC TGAGGAGAAT
    GCACTCCGCG GGGGCTACAG CACCCCCCAG ACGCTGGCCA ACAGGGACCG CTGCCGTGTG
    GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT GCGGACCTGG GGGATGGTGG TTGTTATGGT
    GATCTGTTCT GCAAAGCTCT GAAAACATAC AATATGCTTT GTTTTGGAAT TTATCGGCTG
    AGAGATGCTC ACCTCAGCAC CCCCAGCCAG TGCACGAAGA GGTATGTCAT CACCAACCCC
    CCGTACGAGT TTGAGCTCGT GCCGACGGAC CTGATCTTCT GCTTAATGCA GTTTGACCAC
    AACGCCGGCC AGTCCCGGGC CAGCCTCTCC CATTCCTCCC ACTCGTCCCA GTCCTCTAGC
    AAGAAGAGCT CCTCCGTCCA CTCCATCCCA TCCACGGCAA ACCGACAGAA CCGGCCCAAG
    TCCCGGGAGT CCCGAGACAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842340.1
    CDS169..3756
    Translation

    Target ORF information:

    RefSeq Version XM_024986572.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTGAT ATATTTTGAA GATGAACAGC CGTCGACACT GTCACCCAAA
    AAAAAGCAGC GCAACGGAGG CATGCGGAAC TCACCCAGCT CGTCGCCCAA GCTGATGAGG
    CATGACCCCT TGTTAATTCC TGGCAATGAT CAGATTGACA ACATGGACTC CAATGTGAAG
    AAATATGACT CTACTGGGAT GTTTCACTGG TGTGCGCCCA AGGAGATAGA GAAAGTCATC
    CTGACCCGAA GTGAAGCTGC CATGACCGTC CTGAGCGGCC ACGTGGTGGT CTGCATCTTT
    GGCGACGTCA GCTCTGCCCT GATTGGCCTT CGGAACCTGG TGATGCCGCT CCGTGCCAGC
    AACTTTCACT ACCACGAGCT CAAGCACATC GTGTTTGTGG GCTCCATCGA GTACCTCAAG
    CGGGAATGGG AGACACTGCA TAACTTCCCC AAGGTTTCCA TCTTGCCTGG TACGCCATTA
    AGTCGGGCTG ATTTAAGGGC CGTCAACATC AACCTCTGTG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ATAATATTGA TGATACTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA
    CTCAACATCA AATCTATGCA GTTTGATGAC AGCATCGGGG TCTTGCAGGC TAATTCCCAA
    GGGTTCACAC CTCCAGGAAT GGACCGATCC TCACCAGATA ACAGCCCAGT ACATGGGATG
    TTACGCCAGC CCTCCATCAC GACTGGGGTC AACATCCCCA TCATCACTGA ACTAGCTAAA
    CCTGGCAAGT TGCCTTTGGT ATCAGTCAAT CAGGAAAAAA ACAGTGGGAC GCACATTCTA
    ATGATAACCG AACTGGTGAA CGATACTAAT GTTCAGTTTT TGGACCAAGA CGATGACGAT
    GACCCCGACA CAGAACTGTA CCTCACGCAG CCGTTTGCAT GTGGGACAGC GTTCGCCGTC
    AGTGTCCTGG ATTCACTCAT GAGCGCAACA TACTTCAATG ACAACATCCT TACCCTGATA
    CGGACCCTGG TGACTGGAGG AGCCACACCA GAGCTGGAGG CTTTGATCGC TGAGGAGAAT
    GCACTCCGCG GGGGCTACAG CACCCCCCAG ACGCTGGCCA ACAGGGACCG CTGCCGTGTG
    GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT GCGGACCTGG GGGATGGTGG TTGTTATGGT
    GATCTGTTCT GCAAAGCTCT GAAAACATAC AATATGCTTT GTTTTGGAAT TTATCGGCTG
    AGAGATGCTC ACCTCAGCAC CCCCAGCCAG TGCACGAAGA GGTATGTCAT CACCAACCCC
    CCGTACGAGT TTGAGCTCGT GCCGACGGAC CTGATCTTCT GCTTAATGCA GTTTGACCAC
    AACGCCGGCC AGTCCCGGGC CAGCCTCTCC CATTCCTCCC ACTCGTCCCA GTCCTCTAGC
    AAGAAGAGCT CCTCCGTCCA CTCCATCCCA TCCACGGCAA ACCGACAGAA CCGGCCCAAG
    TCCCGGGAGT CCCGAGACAA ACAGAAAAAA GAAATGGTTT ACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231571
    Clone ID Related Accession (Same CDS sequence) XM_024986573.1
    Accession Version XM_024986573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCAAT GTTTGCCCGC TACGTGCCCG AAATTGCTGC TCTCATCCTG
    AATCGGAATA AATTCGGCGG GACATTTAAC AAACATGGAG GCAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAAGTAC
    GTGCAGGAAG AGCGGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842341.1
    CDS169..3669
    Translation

    Target ORF information:

    RefSeq Version XM_024986573.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGCA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCTTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCAAT GTTTGCCCGC TACGTGCCCG AAATTGCTGC TCTCATCCTG
    AATCGGAATA AATTCGGCGG GACATTTAAC AAACATGGAG GCAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCTCTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTCAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CTACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCAG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CGACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCGCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAAGTAC
    GTGCAGGAAG AGCGGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43733
    Clone ID Related Accession (Same CDS sequence) NM_174680.2
    Accession Version NM_174680.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product calcium-activated potassium channel subunit alpha-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY033472.1. On or before Mar 29, 2005 this sequence version replaced XM_611874.1, NM_174680.1. ##Evidence-Data-START## Transcript exon combination :: AY033472.1, U60105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGGA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCGTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCCTTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTGAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CGACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCGG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CTACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCCCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAAGTAC
    GTGCAGGAAG AGCGGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_777105.1
    CDS275..3775
    Translation

    Target ORF information:

    RefSeq Version NM_174680.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174680.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAATG GTGGCGGCGG CGGCGGCGGC GGCGGCGGAG GCAGCAGTCT TAGAATGAGC 
    AGCAATATCC ACGCGAACCA TCTCAGCCTA GACGCGTCCT CCTCCTCCTC TTCTTCCTCC
    TCCTCCTCTT CCTCGTCGTC CTCGGTCCAC GAGCCCAAGA TGGATGCGCT CATCATCCCG
    GTGACCATGG AGGTGCCGTG CGACAGCCGG GGCCAACGGA TGTGGTGGGC TTTCCTGGCC
    TCCTCCATGG TGACTTTCTT CGGCGGCCTC TTCATCATCT TGCTCTGGAG GACGCTCAAG
    TACCTGTGGA CCGTTTGCTG CCACTGCGGG GGCAAAACGA AGGAGGCCCA GAAGATTAAC
    AATGGCTCAA GCCAGGCAGA TGGCACTCTC AAGCCAGTGG ATGAAAAAGA GGAGACGGTG
    GCAGCCGAGG TCGGCTGGAT GACCTCCGTG AAAGACTGGG CGGGGGTGAT GATATCTGCC
    CAGACGCTGA CTGGCAGAGT CCTGGTTGTC TTAGTCTTTG CTCTCAGCAT TGGTGCACTT
    GTAATATACT TCATAGATTC GTCAAACCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT
    TTCACATTAC AGATCGACAT GGCGTTCAAC GTATTCTTCC TTCTCTACTT TGGCTTGCGG
    TTTATTGCAG CCAACGATAA GCTATGGTTC TGGCTTGAAG TGAACTCTGT GGTAGATTTC
    TTCACGGTCC CCCCTGTGTT TGTGTCCGTG TACTTAAACA GAAGTTGGCT TGGTTTGAGA
    TTTTTAAGAG CTCTCAGACT GATCCAGTTT TCAGAAATTT TACAGTTTCT GAATATCCTG
    AAAACAAGTA ATTCCATCAA GCTGGTGAAT CTGCTCTCCA TTTTTATCAG CACGTGGCTC
    ACAGCAGCCG GGTTCATCCA TTTGGTGGAG AATTCAGGGG ACCCATGGGA AAATTTCCAA
    AACAACCAGG CTCTTACCTA CTGGGAATGT GTCTATCTGC TCATGGTGAC CATGTCCACT
    GTTGGTTACG GGGATGTTTA TGCAAAAACC ACGCTCGGGC GTCTCTTCAT GGTCTTCTTC
    ATCCTCGGGG GACTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA
    AACCGCAAGA AATACGGGGG CTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTGGTC
    TGTGGACACA TCACACTGGA GAGCGTTTCC AACTTCCTGA AGGACTTTCT GCACAAGGAC
    CGGGACGATG TCAATGTGGA GATCGTCTTT CTTCACAACA TCTCCCCTAA CCTGGAGCTG
    GAAGCCTTGT TCAAACGACA TTTTACTCAG GTGGAGTTTT ATCAGGGTTC AGTCCTCAAT
    CCACATGATC TTGCAAGAGT CAAGATAGAG TCAGCAGATG CGTGCCTGAT CCTTGCCAAT
    AAGTACTGCG CAGACCCCGA TGCTGAAGAT GCCTCCAACA TCATGAGAGT CATCTCCATA
    AAGAACTACC ACCCGAAGAT AAGAATCATC ACTCAGATGC TGCAGTATCA CAATAAGGCC
    CATCTGCTAA ACATCCCGAG CTGGAATTGG AAAGAGGGGG ATGATGCAAT CTGCCTCGCA
    GAACTGAAGC TGGGGTTCAT CGCCCAGAGC TGCCTGGCTC AAGGGCTCTC CACCATGCTC
    GCCAACCTCT TCTCCATGAG GTCATTCATA AAGATTGAGG AAGACACGTG GCAGAAATAC
    TACTTGGAAG GAGTCTCAAA TGAAATGTAC ACAGAATATC TCTCCAGTGC CTTCGTGGGT
    CTGTCCTTCC CGACTGTTTG TGAGCTGTGT TTTGTGAAGC TCAAGCTTCT CATGATAGCC
    ATTGAGTACA AGTCCGCCAA TCGAGAGAGC CGTATATTAA TTAATCCTGG AAACCATCTT
    AAGATCCAAG AAGGTACTTT AGGATTTTTC ATCGCAAGTG ATGCCAAAGA AGTTAAAAGG
    GCATTTTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA
    TGCGGCTGCA AACGGCTTGA AGATGAACAG CCGTCGACAC TGTCACCCAA AAAAAAGCAG
    CGCAACGGAG GCATGCGGAA CTCACCCAGC TCGTCGCCCA AGCTGATGAG GCATGACCCC
    TTGTTAATTC CTGGCAATGA TCAGATTGAC AACATGGACT CCAATGTGAA GAAATATGAC
    TCTACTGGGA TGTTTCACTG GTGTGCGCCC AAGGAGATAG AGAAAGTCAT CCTGACCCGA
    AGTGAAGCTG CCATGACCGT CCTGAGCGGC CACGTGGTGG TCTGCATCTT TGGCGACGTC
    AGCTCTGCCC TGATTGGCCT TCGGAACCTG GTGATGCCGC TCCGTGCCAG CAACTTTCAC
    TACCACGAGC TCAAGCACAT CGTGTTTGTG GGCTCCATCG AGTACCTCAA GCGGGAATGG
    GAGACACTGC ATAACTTCCC CAAGGTTTCC ATCTTGCCTG GTACGCCATT AAGTCGGGCT
    GATTTAAGGG CCGTCAACAT CAACCTCTGT GACATGTGCG TTATCCTGTC AGCCAATCAG
    AATAATATTG ATGATACTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC
    AAATCTATGC AGTTTGATGA CAGCATCGGG GTCTTGCAGG CTAATTCCCA AGGGTTCACA
    CCTCCAGGAA TGGACCGATC CTCACCAGAT AACAGCCCGG TACATGGGAT GTTACGCCAG
    CCCTCCATCA CTACTGGGGT CAACATCCCC ATCATCACTG AACTAGTGAA CGATACTAAT
    GTTCAGTTTT TGGACCAAGA CGATGACGAT GACCCCGACA CAGAACTGTA CCTCACGCAG
    CCGTTTGCAT GTGGGACAGC GTTCGCCGTC AGTGTCCTGG ATTCACTCAT GAGCGCAACA
    TACTTCAATG ACAACATCCT TACCCTGATA CGGACCCTGG TGACTGGAGG AGCCACACCA
    GAGCTGGAGG CTTTGATCGC TGAGGAGAAT GCACTCCGCG GGGGCTACAG CACCCCCCAG
    ACGCTGGCCA ACAGGGACCG CTGCCGTGTG GCCCAGTTAG CGCTGCTGGA CGGGCCCTTT
    GCGGACCTGG GGGATGGTGG TTGTTATGGT GATCTGTTCT GCAAAGCTCT GAAAACATAC
    AATATGCTTT GTTTTGGAAT TTATCGGCTG AGAGATGCTC ACCTCAGCAC CCCCAGCCAG
    TGCACGAAGA GGTATGTCAT CACCAACCCC CCGTACGAGT TTGAGCTCGT GCCGACGGAC
    CTGATCTTCT GCTTAATGCA GTTTGACCAC AACGCCGGCC AGTCCCGGGC CAGCCTCTCC
    CATTCCTCCC ACTCGTCCCA GTCCTCTAGC AAGAAGAGCT CCTCCGTCCA CTCCATCCCA
    TCCACGGCAA ACCGACAGAA CCGGCCCAAG TCCCGGGAGT CCCGAGACAA ACAGAAGTAC
    GTGCAGGAAG AGCGGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.