PAFAH1B1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAFAH1B1
Entrez Gene ID	282513
Full Name	platelet activating factor acetylhydrolase 1b regulatory subunit 1
Synonyms	LIS-1,LIS1,MDCR
General protein information	Preferred Names platelet activating factor acetylhydrolase 1b regulatory subunit 1 Names platelet-activating factor acetylhydrolase IB subunit alpha Miller-Dieker syndrome chromosome region PAF acetylhydrolase 45 kDa subunit PAF-AH 45 kDa subunit PAF-AH alpha PAFAH alpha lissencephaly-1 protein platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD) platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979637.1	XP_024835405.1	platelet-activating factor acetylhydrolase IB subunit alpha isoform X1
XM_024979636.1	XP_024835404.1	platelet-activating factor acetylhydrolase IB subunit alpha isoform X1
NM_174663.2	NP_777088.1	platelet-activating factor acetylhydrolase IB subunit alpha

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PAFAH1B1	NP_989655.1
Pan troglodytes (chimpanzee)	PAFAH1B1	NP_001029263.1
Mus musculus (house mouse)	Pafah1b1	NP_038653.1
Danio rerio (zebrafish)	pafah1b1a	NP_958502.1
Caenorhabditis elegans (roundworm)	lis-1	NP_499755.1
Xenopus tropicalis (tropical clawed frog)	pafah1b1	NP_991399.1
Bos taurus (cattle)	PAFAH1B1	NP_777088.1
	PAFAH1B1	XP_001087675.1
Rattus norvegicus (Norway rat)	Pafah1b1	NP_113951.1
Homo sapiens (human)	PAFAH1B1	NP_000421.1
Canis lupus familiaris (dog)	PAFAH1B1	XP_854482.1
Magnaporthe oryzae (rice blast fungus)	MGG_09369	XP_003720251.1
Neurospora crassa	NCU04534	XP_956945.2

Gene Ontology
Bibliography

Related articles in PubMed

PAFAH Ib phospholipase A2 subunits have distinct roles in maintaining Golgi structure and function.
Bechler ME, Brown WJ
Biochimica et biophysica acta1831(3)595-601(2013 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

LIS1 association with dynactin is required for nuclear motility and genomic union in the fertilized mammalian oocyte.
Payne C, St John JC, Ramalho-Santos J, Schatten G
Cell motility and the cytoskeleton56(4)245-51(2003 Dec)

A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function.
Faulkner NE, Dujardin DL, Tai CY, Vaughan KT, O'Connell CB, Wang Y, Vallee RB
Nature cell biology2(11)784-91(2000 Nov)

Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE.
Kitagawa M, Umezu M, Aoki J, Koizumi H, Arai H, Inoue K
FEBS letters479(1-2)57-62(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PAFAH Ib phospholipase A2 subunits have distinct roles in maintaining Golgi structure and function.
Title: PAFAH Ib phospholipase A2 subunits have distinct roles in maintaining Golgi structure and function.

Results suggest that LIS1 is essential to mediate genomic union in a process that involves the dynein-dynactin complex.
Title: LIS1 association with dynactin is required for nuclear motility and genomic union in the fertilized mammalian oocyte.

The following PAFAH1B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAFAH1B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41706	NM_174663.2 Latest version!	Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa226005	XM_024979637.1 Latest version!	Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa226005	XM_024979636.1 Latest version!	Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAFAH1B1 ( NM_174663.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777088.1 Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), mRNA.

Price & Availability↑

CloneID	OBa41706
Clone ID Related Accession (Same CDS sequence)	NM_174663.2
Accession Version	NM_174663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	platelet-activating factor acetylhydrolase IB subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D30615.1. On Jun 3, 2003 this sequence version replaced NM_174663.1. ##Evidence-Data-START## Transcript exon combination :: D30615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCTGT CCCAGAGACA ACGAGATGAA CTAAATCGAG CTATAGCAGA TTATCTTCGT 
TCAAATGGCT ACGAAGCCGC ATATTCAGTT TTTAAAAAGG AAGCTGAATT AGATATGAAT 
GAAGAATTAG ATAAGAAATA TGCTGGTCTT TTGGAAAAAA AATGGACATC TGTTATTAGA 
TTACAAAAGA AGGTTATGGA ATTAGAATCA AAGTTAAATG AAGCAAAAGA AGAATTTACG 
TCGGGTGGAC CTCTTGGTCA GAAAAGAGAC CCAAAAGAAT GGATTCCCCG TCCACCAGAA 
AAATATGCAT TGAGTGGTCA TAGGAGTCCA GTCACTCGAG TCATTTTCCA TCCTGTGTTC 
AGTGTTATGG TCTCTGCTTC AGAGGATGCT ACAATTAAGG TGTGGGATTA TGAGACTGGA 
GATTTTGAAC GAACTCTTAA GGGGCATACA GACTCTGTAG AGGATATTTC ATTCGACCAC 
AGTGGCAAGC TTCTGGCTTC ATGTTCTGCA GATATGACCA TTAAGCTATG GGATTTTCAG 
GGCTTTGAAT GCATCAGAAC CATGCATGGC CATGACCACA ATGTTTCTTC AGTAGCCATC 
ATGCCCAATG GAGATCATAT AGTGTCTGCC TCAAGGGATA AAACTATAAA AATGTGGGAA 
GTGCAAACTG GCTACTGTGT GAAGACATTC ACAGGACACA GAGAATGGGT ACGTATGGTG 
CGGCCAAATC AAGACGGCAC TCTGATAGCC AGCTGTTCCA ATGACCAGAC TGTGCGTGTA 
TGGGTCGTAG CAACAAAGGA ATGCAAGGCT GAGCTTCGAG AACATGAGCA TGTGGTAGAA 
TGCATTTCCT GGGCTCCTGA AAGCTCATAT TCTTCCATCT CTGAAGCAAC AGGATCTGAG 
ACTAAAAAAA GTGGCAAACC TGGGCCATTC TTACTGTCCG GATCCAGGGA CAAGACTATC 
AAGATGTGGG ATGTCAGTAC TGGCATGTGC CTTATGACCC TGGTGGGTCA TGATAACTGG 
GTACGTGGAG TTCTGTTCCA TTCTGGGGGG AAGTTTATTT TGAGTTGCGC TGATGACAAG 
ACCCTGCGCG TGTGGGATTA CAAGAACAAG CGATGCATGA AGACCCTCAA TGCGCATGAA 
CACTTTGTTA CCTCCTTGGA TTTCCATAAG ACGGCCCCAT ATGTGGTTAC TGGCAGTGTA 
GATCAAACAG TAAAGGTGTG GGAGTGTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777088.1
CDS	844..2076
Translation	MVLSQRQRDELNRAIADYLRSNGYEAAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVEDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR

Target ORF information:

RefSeq Version	NM_174663.2
Organism	Bos taurus(cattle)
Definition	Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174663.2

ORF Insert Sequence:

ATGGTGCTGT CCCAGAGACA ACGAGATGAA CTAAATCGAG CTATAGCAGA TTATCTTCGT 
TCAAATGGCT ACGAAGCCGC ATATTCAGTT TTTAAAAAGG AAGCTGAATT AGATATGAAT 
GAAGAATTAG ATAAGAAATA TGCTGGTCTT TTGGAAAAAA AATGGACATC TGTTATTAGA 
TTACAAAAGA AGGTTATGGA ATTAGAATCA AAGTTAAATG AAGCAAAAGA AGAATTTACG 
TCGGGTGGAC CTCTTGGTCA GAAAAGAGAC CCAAAAGAAT GGATTCCCCG TCCACCAGAA 
AAATATGCAT TGAGTGGTCA TAGGAGTCCA GTCACTCGAG TCATTTTCCA TCCTGTGTTC 
AGTGTTATGG TCTCTGCTTC AGAGGATGCT ACAATTAAGG TGTGGGATTA TGAGACTGGA 
GATTTTGAAC GAACTCTTAA GGGGCATACA GACTCTGTAG AGGATATTTC ATTCGACCAC 
AGTGGCAAGC TTCTGGCTTC ATGTTCTGCA GATATGACCA TTAAGCTATG GGATTTTCAG 
GGCTTTGAAT GCATCAGAAC CATGCATGGC CATGACCACA ATGTTTCTTC AGTAGCCATC 
ATGCCCAATG GAGATCATAT AGTGTCTGCC TCAAGGGATA AAACTATAAA AATGTGGGAA 
GTGCAAACTG GCTACTGTGT GAAGACATTC ACAGGACACA GAGAATGGGT ACGTATGGTG 
CGGCCAAATC AAGACGGCAC TCTGATAGCC AGCTGTTCCA ATGACCAGAC TGTGCGTGTA 
TGGGTCGTAG CAACAAAGGA ATGCAAGGCT GAGCTTCGAG AACATGAGCA TGTGGTAGAA 
TGCATTTCCT GGGCTCCTGA AAGCTCATAT TCTTCCATCT CTGAAGCAAC AGGATCTGAG 
ACTAAAAAAA GTGGCAAACC TGGGCCATTC TTACTGTCCG GATCCAGGGA CAAGACTATC 
AAGATGTGGG ATGTCAGTAC TGGCATGTGC CTTATGACCC TGGTGGGTCA TGATAACTGG 
GTACGTGGAG TTCTGTTCCA TTCTGGGGGG AAGTTTATTT TGAGTTGCGC TGATGACAAG 
ACCCTGCGCG TGTGGGATTA CAAGAACAAG CGATGCATGA AGACCCTCAA TGCGCATGAA 
CACTTTGTTA CCTCCTTGGA TTTCCATAAG ACGGCCCCAT ATGTGGTTAC TGGCAGTGTA 
GATCAAACAG TAAAGGTGTG GGAGTGTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAFAH1B1 ( XM_024979637.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835405.1 Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226005
Clone ID Related Accession (Same CDS sequence)	XM_024979637.1 , XM_024979636.1
Accession Version	XM_024979637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	platelet-activating factor acetylhydrolase IB subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGT CCCAGAGACA ACGAGATGAA CTAAATCGAG CTATAGCAGA TTATCTTCGT 
TCAAATGGCT ACGAAGAAGC ATATTCAGTT TTTAAAAAGG AAGCTGAATT AGATATGAAT 
GAAGAATTAG ATAAGAAATA TGCTGGTCTT TTGGAAAAAA AATGGACATC TGTTATTAGA 
TTACAAAAGA AGGTTATGGA ATTAGAATCA AAGTTAAATG AAGCAAAAGA AGAATTTACG 
TCGGGTGGAC CTCTTGGTCA GAAAAGAGAC CCAAAAGAAT GGATTCCCCG TCCACCAGAA 
AAATATGCAT TGAGTGGTCA TAGGAGTCCA GTCACTCGAG TCATTTTCCA TCCTGTGTTC 
AGTGTTATGG TCTCTGCTTC AGAGGATGCT ACAATTAAGG TGTGGGATTA TGAGACTGGA 
GATTTTGAAC GAACTCTTAA GGGGCATACA GACTCTGTAC AGGATATTTC ATTCGACCAC 
AGTGGCAAGC TTCTGGCTTC ATGTTCTGCA GATATGACCA TTAAGCTATG GGATTTTCAG 
GGCTTTGAAT GCATCAGAAC CATGCATGGC CATGACCACA ATGTTTCTTC AGTAGCCATC 
ATGCCCAATG GAGATCATAT AGTGTCTGCC TCAAGGGATA AAACTATAAA AATGTGGGAA 
GTGCAAACTG GCTACTGTGT GAAGACATTC ACAGGACACA GAGAATGGGT ACGTATGGTG 
CGGCCAAATC AAGACGGCAC TCTGATAGCC AGCTGTTCCA ATGACCAGAC TGTGCGTGTA 
TGGGTCGTAG CAACAAAGGA ATGCAAGGCT GAGCTTCGAG AACATGAGCA TGTGGTAGAA 
TGCATTTCCT GGGCTCCTGA AAGCTCATAT TCTTCCATCT CTGAAGCAAC AGGATCTGAG 
ACTAAAAAAA GTGGCAAACC TGGGCCATTC TTACTGTCCG GATCCAGGGA CAAGACTATC 
AAGATGTGGG ATGTCAGTAC TGGCATGTGC CTTATGACCC TGGTGGGTCA TGATAACTGG 
GTACGTGGAG TTCTGTTCCA TTCTGGGGGG AAGTTTATTT TGAGTTGCGC TGATGACAAG 
ACCCTGCGCG TGTGGGATTA CAAGAACAAG CGATGCATGA AGACCCTCAA TGCGCATGAA 
CACTTTGTTA CCTCCTTGGA TTTCCATAAG ACGGCCCCAT ATGTGGTTAC TGGCAGTGTA 
GATCAAACAG TAAAGGTGTG GGAGTGTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835405.1
CDS	847..2079
Translation	MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR

Target ORF information:

RefSeq Version	XM_024979637.1
Organism	Bos taurus(cattle)
Definition	Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979637.1

ORF Insert Sequence:

ATGGTGCTGT CCCAGAGACA ACGAGATGAA CTAAATCGAG CTATAGCAGA TTATCTTCGT 
TCAAATGGCT ACGAAGAAGC ATATTCAGTT TTTAAAAAGG AAGCTGAATT AGATATGAAT 
GAAGAATTAG ATAAGAAATA TGCTGGTCTT TTGGAAAAAA AATGGACATC TGTTATTAGA 
TTACAAAAGA AGGTTATGGA ATTAGAATCA AAGTTAAATG AAGCAAAAGA AGAATTTACG 
TCGGGTGGAC CTCTTGGTCA GAAAAGAGAC CCAAAAGAAT GGATTCCCCG TCCACCAGAA 
AAATATGCAT TGAGTGGTCA TAGGAGTCCA GTCACTCGAG TCATTTTCCA TCCTGTGTTC 
AGTGTTATGG TCTCTGCTTC AGAGGATGCT ACAATTAAGG TGTGGGATTA TGAGACTGGA 
GATTTTGAAC GAACTCTTAA GGGGCATACA GACTCTGTAC AGGATATTTC ATTCGACCAC 
AGTGGCAAGC TTCTGGCTTC ATGTTCTGCA GATATGACCA TTAAGCTATG GGATTTTCAG 
GGCTTTGAAT GCATCAGAAC CATGCATGGC CATGACCACA ATGTTTCTTC AGTAGCCATC 
ATGCCCAATG GAGATCATAT AGTGTCTGCC TCAAGGGATA AAACTATAAA AATGTGGGAA 
GTGCAAACTG GCTACTGTGT GAAGACATTC ACAGGACACA GAGAATGGGT ACGTATGGTG 
CGGCCAAATC AAGACGGCAC TCTGATAGCC AGCTGTTCCA ATGACCAGAC TGTGCGTGTA 
TGGGTCGTAG CAACAAAGGA ATGCAAGGCT GAGCTTCGAG AACATGAGCA TGTGGTAGAA 
TGCATTTCCT GGGCTCCTGA AAGCTCATAT TCTTCCATCT CTGAAGCAAC AGGATCTGAG 
ACTAAAAAAA GTGGCAAACC TGGGCCATTC TTACTGTCCG GATCCAGGGA CAAGACTATC 
AAGATGTGGG ATGTCAGTAC TGGCATGTGC CTTATGACCC TGGTGGGTCA TGATAACTGG 
GTACGTGGAG TTCTGTTCCA TTCTGGGGGG AAGTTTATTT TGAGTTGCGC TGATGACAAG 
ACCCTGCGCG TGTGGGATTA CAAGAACAAG CGATGCATGA AGACCCTCAA TGCGCATGAA 
CACTTTGTTA CCTCCTTGGA TTTCCATAAG ACGGCCCCAT ATGTGGTTAC TGGCAGTGTA 
GATCAAACAG TAAAGGTGTG GGAGTGTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAFAH1B1 ( XM_024979636.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835404.1 Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226005
Clone ID Related Accession (Same CDS sequence)	XM_024979637.1 , XM_024979636.1
Accession Version	XM_024979636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	platelet-activating factor acetylhydrolase IB subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGT CCCAGAGACA ACGAGATGAA CTAAATCGAG CTATAGCAGA TTATCTTCGT 
TCAAATGGCT ACGAAGAAGC ATATTCAGTT TTTAAAAAGG AAGCTGAATT AGATATGAAT 
GAAGAATTAG ATAAGAAATA TGCTGGTCTT TTGGAAAAAA AATGGACATC TGTTATTAGA 
TTACAAAAGA AGGTTATGGA ATTAGAATCA AAGTTAAATG AAGCAAAAGA AGAATTTACG 
TCGGGTGGAC CTCTTGGTCA GAAAAGAGAC CCAAAAGAAT GGATTCCCCG TCCACCAGAA 
AAATATGCAT TGAGTGGTCA TAGGAGTCCA GTCACTCGAG TCATTTTCCA TCCTGTGTTC 
AGTGTTATGG TCTCTGCTTC AGAGGATGCT ACAATTAAGG TGTGGGATTA TGAGACTGGA 
GATTTTGAAC GAACTCTTAA GGGGCATACA GACTCTGTAC AGGATATTTC ATTCGACCAC 
AGTGGCAAGC TTCTGGCTTC ATGTTCTGCA GATATGACCA TTAAGCTATG GGATTTTCAG 
GGCTTTGAAT GCATCAGAAC CATGCATGGC CATGACCACA ATGTTTCTTC AGTAGCCATC 
ATGCCCAATG GAGATCATAT AGTGTCTGCC TCAAGGGATA AAACTATAAA AATGTGGGAA 
GTGCAAACTG GCTACTGTGT GAAGACATTC ACAGGACACA GAGAATGGGT ACGTATGGTG 
CGGCCAAATC AAGACGGCAC TCTGATAGCC AGCTGTTCCA ATGACCAGAC TGTGCGTGTA 
TGGGTCGTAG CAACAAAGGA ATGCAAGGCT GAGCTTCGAG AACATGAGCA TGTGGTAGAA 
TGCATTTCCT GGGCTCCTGA AAGCTCATAT TCTTCCATCT CTGAAGCAAC AGGATCTGAG 
ACTAAAAAAA GTGGCAAACC TGGGCCATTC TTACTGTCCG GATCCAGGGA CAAGACTATC 
AAGATGTGGG ATGTCAGTAC TGGCATGTGC CTTATGACCC TGGTGGGTCA TGATAACTGG 
GTACGTGGAG TTCTGTTCCA TTCTGGGGGG AAGTTTATTT TGAGTTGCGC TGATGACAAG 
ACCCTGCGCG TGTGGGATTA CAAGAACAAG CGATGCATGA AGACCCTCAA TGCGCATGAA 
CACTTTGTTA CCTCCTTGGA TTTCCATAAG ACGGCCCCAT ATGTGGTTAC TGGCAGTGTA 
GATCAAACAG TAAAGGTGTG GGAGTGTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835404.1
CDS	586..1818
Translation	MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR

Target ORF information:

RefSeq Version	XM_024979636.1
Organism	Bos taurus(cattle)
Definition	Bos taurus platelet activating factor acetylhydrolase 1b regulatory subunit 1 (PAFAH1B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979636.1

ORF Insert Sequence:

ATGGTGCTGT CCCAGAGACA ACGAGATGAA CTAAATCGAG CTATAGCAGA TTATCTTCGT 
TCAAATGGCT ACGAAGAAGC ATATTCAGTT TTTAAAAAGG AAGCTGAATT AGATATGAAT 
GAAGAATTAG ATAAGAAATA TGCTGGTCTT TTGGAAAAAA AATGGACATC TGTTATTAGA 
TTACAAAAGA AGGTTATGGA ATTAGAATCA AAGTTAAATG AAGCAAAAGA AGAATTTACG 
TCGGGTGGAC CTCTTGGTCA GAAAAGAGAC CCAAAAGAAT GGATTCCCCG TCCACCAGAA 
AAATATGCAT TGAGTGGTCA TAGGAGTCCA GTCACTCGAG TCATTTTCCA TCCTGTGTTC 
AGTGTTATGG TCTCTGCTTC AGAGGATGCT ACAATTAAGG TGTGGGATTA TGAGACTGGA 
GATTTTGAAC GAACTCTTAA GGGGCATACA GACTCTGTAC AGGATATTTC ATTCGACCAC 
AGTGGCAAGC TTCTGGCTTC ATGTTCTGCA GATATGACCA TTAAGCTATG GGATTTTCAG 
GGCTTTGAAT GCATCAGAAC CATGCATGGC CATGACCACA ATGTTTCTTC AGTAGCCATC 
ATGCCCAATG GAGATCATAT AGTGTCTGCC TCAAGGGATA AAACTATAAA AATGTGGGAA 
GTGCAAACTG GCTACTGTGT GAAGACATTC ACAGGACACA GAGAATGGGT ACGTATGGTG 
CGGCCAAATC AAGACGGCAC TCTGATAGCC AGCTGTTCCA ATGACCAGAC TGTGCGTGTA 
TGGGTCGTAG CAACAAAGGA ATGCAAGGCT GAGCTTCGAG AACATGAGCA TGTGGTAGAA 
TGCATTTCCT GGGCTCCTGA AAGCTCATAT TCTTCCATCT CTGAAGCAAC AGGATCTGAG 
ACTAAAAAAA GTGGCAAACC TGGGCCATTC TTACTGTCCG GATCCAGGGA CAAGACTATC 
AAGATGTGGG ATGTCAGTAC TGGCATGTGC CTTATGACCC TGGTGGGTCA TGATAACTGG 
GTACGTGGAG TTCTGTTCCA TTCTGGGGGG AAGTTTATTT TGAGTTGCGC TGATGACAAG 
ACCCTGCGCG TGTGGGATTA CAAGAACAAG CGATGCATGA AGACCCTCAA TGCGCATGAA 
CACTTTGTTA CCTCCTTGGA TTTCCATAAG ACGGCCCCAT ATGTGGTTAC TGGCAGTGTA 
GATCAAACAG TAAAGGTGTG GGAGTGTCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAFAH Ib phospholipase A2 subunits have distinct roles in maintaining Golgi structure and function.
Biochimica et biophysica acta1831(3)595-601(2013 Mar)
Bechler ME,Brown WJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

LIS1 association with dynactin is required for nuclear motility and genomic union in the fertilized mammalian oocyte.
Cell motility and the cytoskeleton56(4)245-51(2003 Dec)
Payne C,St John JC,Ramalho-Santos J,Schatten G

A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function.
Nature cell biology2(11)784-91(2000 Nov)
Faulkner NE,Dujardin DL,Tai CY,Vaughan KT,O'Connell CB,Wang Y,Vallee RB

Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE.
FEBS letters479(1-2)57-62(2000 Aug)
Kitagawa M,Umezu M,Aoki J,Koizumi H,Arai H,Inoue K