CACNA1G cDNA ORF clone, Bos taurus(cattle)

The following CACNA1G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa45434 XM_010816118.2
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22-24 $1371.30
$1959.00
OBa93904 XM_015458772.1
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa216272 NM_001193140.2
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa238994 NM_001193140.3
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225991 XM_005220487.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225992 XM_010816118.3
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225993 XM_015458770.2
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225994 XM_005220488.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225995 XM_015458771.2
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa225996 XM_024979634.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa225997 XM_005220494.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa225998 XM_005220497.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa225999 XM_005220499.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa226000 XM_005220501.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa226001 XM_005220502.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa226002 XM_005220505.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa226003 XM_024979635.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa226004 XM_005220507.4
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa93902 XM_015458770.1
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa93903 XM_015458771.1
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa40889 NM_001193140.1
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa29847 XM_005220487.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa29848 XM_005220488.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa29854 XM_005220494.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa29857 XM_005220497.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa29859 XM_005220499.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa29861 XM_005220501.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa29862 XM_005220502.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa29865 XM_005220505.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa29867 XM_005220507.3
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa45434
    Clone ID Related Accession (Same CDS sequence) XM_010816118.2
    Accession Version XM_010816118.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG
    GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC
    TCTGCCTTCC CTCCTGCCTG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG
    ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG
    ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT
    ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC
    AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG
    AGCACTGCCG AGGGGTCCCT AGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC
    TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA
    GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG
    CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG
    ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT
    GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT
    CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC
    CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG
    GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC
    CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG
    CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT
    GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA
    GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG
    AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT
    CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG
    CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG
    GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814420.1
    CDS731..7819
    Translation

    Target ORF information:

    RefSeq Version XM_010816118.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816118.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG
    GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC
    TCTGCCTTCC CTCCTGCCTG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG
    ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG
    ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT
    ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC
    AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG
    AGCACTGCCG AGGGGTCCCT AGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC
    TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA
    GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG
    CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG
    ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT
    GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT
    CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC
    CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG
    GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC
    CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG
    CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT
    GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA
    GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG
    AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT
    CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG
    CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG
    GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93904
    Clone ID Related Accession (Same CDS sequence) XM_015458772.1
    Accession Version XM_015458772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG
    ACCCTCCTGC CTCGATGTGG AGAGGTAAGC AGGCCCTTGG CTGTCCCCAG AGATGGCTGC
    CCACACTTGC AGCGTGGGGC CACCCCAGCA AGCCACTGGC ACACTTGCTG GCTCATCTGT
    CCCGGTGCCT CCAGCTTGGC TGGGACTTCC CTTCCTGGCT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314258.1
    CDS734..6718
    Translation

    Target ORF information:

    RefSeq Version XM_015458772.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG
    ACCCTCCTGC CTCGATGTGG AGAGGTAAGC AGGCCCTTGG CTGTCCCCAG AGATGGCTGC
    CCACACTTGC AGCGTGGGGC CACCCCAGCA AGCCACTGGC ACACTTGCTG GCTCATCTGT
    CCCGGTGCCT CCAGCTTGGC TGGGACTTCC CTTCCTGGCT TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216272
    Clone ID Related Accession (Same CDS sequence) NM_001193140.2
    Accession Version NM_001193140.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02049035.1, DAAA02049034.1 and DAAA02049033.1. On Jun 30, 2017 this sequence version replaced NM_001193140.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC
    CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC
    TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG
    GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT
    GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG
    TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT
    GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT
    GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC
    TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG
    CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA
    CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT
    TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC
    TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG
    CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC
    CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG
    GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT
    GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA
    TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC
    CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG
    AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG
    AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC
    AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC
    CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180069.1
    CDS1..7143
    Translation

    Target ORF information:

    RefSeq Version NM_001193140.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193140.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC
    CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC
    TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG
    GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT
    GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG
    TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT
    GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT
    GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC
    TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG
    CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA
    CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT
    TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC
    TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG
    CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC
    CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG
    GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT
    GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA
    TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC
    CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG
    AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG
    AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC
    AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC
    CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238994
    Clone ID Related Accession (Same CDS sequence) NM_001193140.3
    Accession Version NM_001193140.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On May 29, 2018 this sequence version replaced NM_001193140.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC
    CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC
    TTCCCTCCTG CCCGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG
    GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT
    GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG
    TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT
    GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT
    GCCGAGGGGT CCCTGGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC
    TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG
    CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA
    CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT
    TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC
    TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG
    CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC
    CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG
    GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT
    GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA
    TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC
    CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG
    AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG
    AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC
    AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC
    CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180069.2
    CDS1..7143
    Translation

    Target ORF information:

    RefSeq Version NM_001193140.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193140.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC
    CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC
    TTCCCTCCTG CCCGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG
    GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT
    GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG
    TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT
    GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT
    GCCGAGGGGT CCCTGGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC
    TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG
    CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA
    CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT
    TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC
    TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG
    CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC
    CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG
    GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT
    GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA
    TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC
    CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG
    AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG
    AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC
    AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC
    CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225991
    Clone ID Related Accession (Same CDS sequence) XM_005220487.4
    Accession Version XM_005220487.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220487.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC
    ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG
    GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC
    CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220544.2
    CDS1115..8224
    Translation

    Target ORF information:

    RefSeq Version XM_005220487.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220487.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC
    ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG
    GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC
    CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225992
    Clone ID Related Accession (Same CDS sequence) XM_010816118.3
    Accession Version XM_010816118.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG
    GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC
    TCTGCCTTCC CTCCTGCCCG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG
    ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG
    ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT
    ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC
    AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG
    AGCACTGCCG AGGGGTCCCT GGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC
    TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA
    GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG
    CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG
    ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT
    GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT
    CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC
    CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG
    GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC
    CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG
    CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT
    GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA
    GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG
    AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT
    CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG
    CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG
    GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814420.2
    CDS1115..8203
    Translation

    Target ORF information:

    RefSeq Version XM_010816118.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816118.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG
    GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC
    TCTGCCTTCC CTCCTGCCCG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG
    ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG
    ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT
    ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC
    AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG
    AGCACTGCCG AGGGGTCCCT GGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC
    TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA
    GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG
    CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG
    ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT
    GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT
    CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC
    CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG
    GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC
    CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG
    CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT
    GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA
    GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG
    AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT
    CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG
    CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG
    GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225993
    Clone ID Related Accession (Same CDS sequence) XM_015458770.2
    Accession Version XM_015458770.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG
    GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT
    GCCCGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT
    TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG
    CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT
    GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC
    CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG
    TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC
    CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG
    CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC
    TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC
    ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG
    CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC
    AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC
    AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC
    TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC
    CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG
    GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC
    CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC
    AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC
    CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC
    AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA
    GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314256.2
    CDS1115..8188
    Translation

    Target ORF information:

    RefSeq Version XM_015458770.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458770.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG
    GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT
    GCCCGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT
    TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG
    CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT
    GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC
    CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG
    TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC
    CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG
    CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC
    TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC
    ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG
    CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC
    AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC
    AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC
    TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC
    CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG
    GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC
    CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC
    AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC
    CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC
    AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA
    GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225994
    Clone ID Related Accession (Same CDS sequence) XM_005220488.4
    Accession Version XM_005220488.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220488.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC
    CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA
    GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC CGCAGCCCAG GCGGCTCCGA CCCACAGATC
    CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC
    TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC
    CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220545.2
    CDS772..7812
    Translation

    Target ORF information:

    RefSeq Version XM_005220488.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220488.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC
    CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA
    GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC CGCAGCCCAG GCGGCTCCGA CCCACAGATC
    CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC
    TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC
    CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225995
    Clone ID Related Accession (Same CDS sequence) XM_015458771.2
    Accession Version XM_015458771.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT
    GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC
    ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC
    ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG
    CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT GGGACACAGG
    GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA
    GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT
    GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG
    AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC
    AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC
    TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC
    TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC
    CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC
    CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC
    AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG
    CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC
    TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC
    CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC
    AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC
    TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC
    TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314257.1
    CDS772..7770
    Translation

    Target ORF information:

    RefSeq Version XM_015458771.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458771.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT
    GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC
    ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC
    ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG
    CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT GGGACACAGG
    GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA
    GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT
    GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG
    AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC
    AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC
    TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC
    TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC
    CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC
    CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC
    AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG
    CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC
    TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC
    CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC
    AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC
    TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC
    TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225996
    Clone ID Related Accession (Same CDS sequence) XM_024979634.1
    Accession Version XM_024979634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG
    ACCCTCCTGC CTCGATGTGG AGAGATGGAG TCCCACCCTG AGGAGGTGCC CGTCATGCTA
    GGACCAGATC TGCTGACTGT GCGCAAGTCT GGGGTCAGCC GAACGCACTC TCTGCCCAAT
    GACAGCTACA TGTGCCGGGA TGGGAGCACT GCCGAGGGGT CCCTGGGACA CAGGGGCTGG
    GGGCTCCCCA GAGCTCAGTC AGGCTCCATC TTGTCCGTCC ACTCCCAGCC AGCAGACACC
    AGCTACATCC CGCAGCTTCC CAAAGATGCG CCCCATCTGC TCCAGCCCCA TGGTGCTCCC
    ACCTGGGGCA CCATCCCCAA ATTGCCCCCA CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA
    CTCAGGCGCC AGGCAGCAAT ACGGACGGAT TCCCTGGACA TTCAGGGCCT GGGCAGCAGG
    GAAGACCTGC TGTCAGAGGT GAGTGGGCCC TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT
    TGGGGCCACT CCAGCACCCA GGCTCAGCAG CATTCCCGCA GCCAGAGCAA GATCTTGAAG
    CACATGCCCC CGTCAGCCCC CTGCCCAGGC CCAGAACTCA AGTGGGCCAA GGGCCCACCA
    GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG
    CCCACGGACA GTCAGGAGGA AGCCCCGTCT GCACGGGACC TGAAGAAGTG CTACAGCGTG
    GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA TCCTGGCTGG ATGAACAGAG GAGGCACTCC
    ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC CAACCCGCCC TGGGCCCTGA TCCCTCGAGC
    CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG AGCCGGCCCA AGAAGAAACT CAGCCCACCC
    AGCATCTCCA TAGACCCCCC AGAGAGCCAG AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT
    GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC AGCGACTCCA AGGATCCCTT GGCCTCTGGC
    CCTCCTGACA GCATGGCTGC CTCGCCCTCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT
    TTATCCTCTG ACCCAGCAGA CCTGGACCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835402.1
    CDS771..7703
    Translation

    Target ORF information:

    RefSeq Version XM_024979634.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG
    ACCCTCCTGC CTCGATGTGG AGAGATGGAG TCCCACCCTG AGGAGGTGCC CGTCATGCTA
    GGACCAGATC TGCTGACTGT GCGCAAGTCT GGGGTCAGCC GAACGCACTC TCTGCCCAAT
    GACAGCTACA TGTGCCGGGA TGGGAGCACT GCCGAGGGGT CCCTGGGACA CAGGGGCTGG
    GGGCTCCCCA GAGCTCAGTC AGGCTCCATC TTGTCCGTCC ACTCCCAGCC AGCAGACACC
    AGCTACATCC CGCAGCTTCC CAAAGATGCG CCCCATCTGC TCCAGCCCCA TGGTGCTCCC
    ACCTGGGGCA CCATCCCCAA ATTGCCCCCA CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA
    CTCAGGCGCC AGGCAGCAAT ACGGACGGAT TCCCTGGACA TTCAGGGCCT GGGCAGCAGG
    GAAGACCTGC TGTCAGAGGT GAGTGGGCCC TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT
    TGGGGCCACT CCAGCACCCA GGCTCAGCAG CATTCCCGCA GCCAGAGCAA GATCTTGAAG
    CACATGCCCC CGTCAGCCCC CTGCCCAGGC CCAGAACTCA AGTGGGCCAA GGGCCCACCA
    GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG
    CCCACGGACA GTCAGGAGGA AGCCCCGTCT GCACGGGACC TGAAGAAGTG CTACAGCGTG
    GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA TCCTGGCTGG ATGAACAGAG GAGGCACTCC
    ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC CAACCCGCCC TGGGCCCTGA TCCCTCGAGC
    CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG AGCCGGCCCA AGAAGAAACT CAGCCCACCC
    AGCATCTCCA TAGACCCCCC AGAGAGCCAG AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT
    GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC AGCGACTCCA AGGATCCCTT GGCCTCTGGC
    CCTCCTGACA GCATGGCTGC CTCGCCCTCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT
    TTATCCTCTG ACCCAGCAGA CCTGGACCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225997
    Clone ID Related Accession (Same CDS sequence) XM_005220494.4
    Accession Version XM_005220494.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220551.1
    CDS771..7700
    Translation

    Target ORF information:

    RefSeq Version XM_005220494.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220494.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225998
    Clone ID Related Accession (Same CDS sequence) XM_005220497.4
    Accession Version XM_005220497.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT
    CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC
    ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC
    AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC
    CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC
    ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC
    CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG
    CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC
    TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC
    CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA
    AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC
    AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG
    AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC
    AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT
    CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC
    ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC
    CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC
    AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT
    GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220554.1
    CDS769..7632
    Translation

    Target ORF information:

    RefSeq Version XM_005220497.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220497.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT
    CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC
    ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC
    AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC
    CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC
    ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC
    CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG
    CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC
    TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC
    CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA
    AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC
    AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG
    AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC
    AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT
    CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC
    ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC
    CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC
    AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT
    GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225999
    Clone ID Related Accession (Same CDS sequence) XM_005220499.4
    Accession Version XM_005220499.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG
    GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA
    ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC
    CTGGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC
    TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC
    CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC
    CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT
    CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG
    GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC
    CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG
    TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG
    ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG
    AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT
    GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG
    GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG
    AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT
    CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG
    GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC
    GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220556.1
    CDS768..7598
    Translation

    Target ORF information:

    RefSeq Version XM_005220499.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220499.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG
    GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA
    ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC
    CTGGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC
    TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC
    CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC
    CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT
    CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG
    GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC
    CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG
    TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG
    ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG
    AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT
    GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG
    GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG
    AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT
    CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG
    GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC
    GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226000
    Clone ID Related Accession (Same CDS sequence) XM_005220501.4
    Accession Version XM_005220501.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220501.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220558.1
    CDS768..7577
    Translation

    Target ORF information:

    RefSeq Version XM_005220501.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220501.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226001
    Clone ID Related Accession (Same CDS sequence) XM_005220502.4
    Accession Version XM_005220502.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220502.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT
    GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG
    GATGGGAGCA CTGCCGAGGG GTCCCTGGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG
    TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT
    CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC
    AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA
    ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG
    GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC
    CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC
    CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA
    GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG
    GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG
    CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG
    GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA
    GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC
    CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG
    AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT
    GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA
    GACCTGGACC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220559.1
    CDS768..7562
    Translation

    Target ORF information:

    RefSeq Version XM_005220502.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220502.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT
    GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG
    GATGGGAGCA CTGCCGAGGG GTCCCTGGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG
    TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT
    CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC
    AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA
    ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG
    GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC
    CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC
    CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA
    GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG
    GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG
    CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG
    GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA
    GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC
    CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG
    AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT
    GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA
    GACCTGGACC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226002
    Clone ID Related Accession (Same CDS sequence) XM_005220505.4
    Accession Version XM_005220505.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC
    GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT
    CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTGGGACAC
    AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA
    GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT
    GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT
    CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG
    GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC
    TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG
    ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG
    GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA
    GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC
    TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG
    AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT
    CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC
    AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA
    CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG
    GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT
    CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220562.1
    CDS768..7529
    Translation

    Target ORF information:

    RefSeq Version XM_005220505.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220505.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC
    GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT
    CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTGGGACAC
    AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA
    GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT
    GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT
    CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG
    GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC
    TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG
    ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG
    GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA
    GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC
    TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG
    AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT
    CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC
    AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA
    CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG
    GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT
    CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226003
    Clone ID Related Accession (Same CDS sequence) XM_024979635.1
    Accession Version XM_024979635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGTGGTGA AGATGGTGGC CTTGGGCATC TTTGGAAAAA AATGTTACCT GGGAGACACT 
    TGGAACCGGC TTGACTTTTT CATCGTCATT GCAGGGATGC TGGAGTATTC GCTGGACCTG
    CAGAATGTCA GCTTCTCAGC TGTCAGGACA GTCCGTGTGC TTCGACCCCT CAGGGCCATT
    AACCGGGTGC CCAGCATGCG CATCCTCGTC ACGCTGCTGC TGGACACGCT GCCCATGCTG
    GGCAACGTCC TGCTGCTCTG CTTCTTCGTC TTCTTCATCT TCGGCATCGT TGGTGTCCAG
    CTGTGGGCAG GACTGCTTCG TAACCGATGC TTCCTGCCTG AGAATTTCAG CCTCCCCCTG
    AGCGTGGACT TGGAGCGCTA CTACCAGACT GAGAACGAGG ATGAGAACCC ATTCATCTGC
    TCCCAGCCTC GTGAAAACGG CATGCGCTCC TGCAGGAGTG TGCCCACGCT GCGCGGGGAC
    GGGGGTGGCG GCCCACCCTG CGGCCTGGAC TACGAGGCCT ACAACAGCTC CAGCAACACC
    ACCTGTGTGA ACTGGAACCA GTACTACACC AACTGCTCCG CGGGGGAGCA CAACCCCTTC
    AAGGGTGCCA TCAACTTCGA CAACATTGGC TATGCCTGGA TCGCCATCTT CCAGGTCATC
    ACGCTGGAGG GCTGGGTCGA CATCATGTAC TTTGTGATGG ATGCTCACTC CTTCTACAAC
    TTCATCTACT TCATCCTTCT CATCATTGTG GGCTCTTTCT TTATGATCAA CCTGTGCCTG
    GTGGTGATTG CCACGCAGTT CTCAGAGACC AAGCAGCGGG AGAGCCAGCT GATGCGGGAG
    CAGCGCGTGA GATTCCTTTC CAACGCCAGC ACCCTGGCCA GCTTCTCCGA GCCCGGCAGC
    TGCTATGAGG AGCTGCTCAA GTACCTCGTG TATATCCTCC GGAAAGCAGC CCGAAGGCTG
    GCCCAGGTCT CCCGGGCGGC GGGCGTGCGG GTTGGCCTGC TGAGCAGCCC AGCGCCCCGT
    GGGGGCCAGG AGCCCCAGCC CAGTGGCGGC TGCCCTCGCC CCCACCGCCG TCCATCCGTC
    CACCATCTGG TGCACCACCA CCACCACCAC CACTACCACT TGGGCAATGG GACTCTGCGG
    GCCCCTCGGC CCAGCCCGCA GATCCAGGAC AGAGATGCCA GTGGGTCCCG CCGGCTCGTG
    CTGCCACCAC CCTCAACTCC TGCCCTTTCC AGGGGCCCTC CAGGGGGTGC AGAGGCTGTG
    CACAGCTTCT ACCATGCTGA TTGCCACTTG GAGCCAGTCC GCTGCCAGGC ACCCCCTCCC
    AGGTCGCCAT CGGAGGCATC GGGCAGGACC GTGGACAGTG GGAAGGTGTA CCCCACCGTG
    CACACCAGCC CTCCACCTGA GATGCTCAAA GAGAAGGCAC TAATGGAGGT GGCGCCCGCC
    TCTGGACCCA CCACCCTCAC CAGCCTCAAC ATCCCGCCCA GGCCCTACAG CTCTATGCAC
    AAGCTGCTGG AGACCCAGAG CACAGGCGCC TGCCAAAGCT CTTGCAAGAT CTCCAGCCCT
    TGCTTGAGGG CAGCCAGCGG AGCCTGTGGC CCAGACAGCT GTCCCTACTG TGCCCGGACC
    GGGGCAGGGG AGGTGGGGCT CACTGGCCAC GAGATGCCTG ACTCAGACAG TGAGGCGGTT
    TATGAGTTCA CACAAGATGC TCAGCATGGC GACCTCCGGG ATCCCCACAG TCGGCGTCGA
    CGGAGCCTGG GGCTGGATGT GGAGCCCAAC TCTGTGCTGG CCTTCTGGAG GCTGATCTGT
    GACACCTTCC GGAAGATTGT GGACAGCAAG TACTTTGGTC GGGGAATCAT GATTGCTATT
    CTGGTCAACA CGCTCAGCAT GGGCATCGAA TACCACGAGC AGCCTGAGGA GCTCACCAAT
    GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTTG CCCTGGAGAT GCTGCTGAAG
    CTGCTAGTAT ATGGTCCCTT TGGCTACATT AAGAACCCCT ACAACATCTT TGATGGCGTC
    ATTGTTGTCA TCAGCGTGTG GGAGATCGTG GGCCAGCAGG GGGGCGGCCT GTCGGTGCTG
    CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTGGTGCGCT TCTTGCCGGC GCTGCAGCGG
    CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG
    CTCTTCATCT TCATCTTCAG CATCCTGGGT ATGCATCTCT TCGGCTGCAA ATTTGCATCT
    GAGCGGGATG GGGACACGTT GCCGGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATC
    GTCACTGTCT TTCAGATCCT GACTCAGGAG GACTGGAACA AGGTGCTCTA CAACGGAATG
    GCCTCCACGT CATCCTGGGC TGCCCTTTAC TTCATCGCCC TCATGACCTT CGGCAACTAT
    GTGCTCTTCA ACCTGCTCGT TGCCATTCTG GTGGAGGGCT TCCAAACGGA GGAAATCAGC
    AAACGGGAAG ATGCGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCGA CTCCCAGGGG
    GGAGATGCCA CCAAGTCAGA GTCGGAACCT GATTTCTTCT CGCCCAGCCT GGATGGTGAT
    GGGGACAGGA AGAAGCGCTT GGCCTTGGTG TCTCTGGGAG AGCACCCGGA GCTGCGGAGG
    AGCTTGTTAC CACCGCTCAT CATCCACACG GCTGCCACTC CCATGTCATT GCCCAAAAGC
    TCCAGCAGCG GCCTGGCCGA GGTTCTGGGC CCCCCCTCCC GCCGCACCAG CAGCAGTGGG
    TCGGCTGAGC CAGGAGCACC TCACGAGATG AAGTCACCGC CAAGTGTCCG CAGTTCCCCC
    CACAGCCCCT GGAGTGCAGC AAGCAGCTGG ACGAGCAGGC GCTCCAGCAG GAACAGCCTG
    GGCCGTGCCC CCAGCCTGAA GCGAAGGAGC CCAAGTGGGG AGCGGAGGTC CTTGTTGTCG
    GGTGAGGGCC GGGAGAGCCA GGATGAGGAG GAGAGCTCGG AAGAGGAGCG CACTAGCCCA
    GCGGGCAGTG ACCGCCACCA CAGGGGCTCC CTGGAGAGGG AAGCTAAGAG CTCCTTTGAC
    CTACCGGACA CACTGCAGGT TCCCGGGCTG TACCGCACAG CCAGCGGCCG CAGCTCAGCC
    TCTGAGCACC AGGACTGCAA TGGCAATTCG GCCGTGGGAC GCCTGAGTCA GGCTGTGCGG
    CCCGATGACC CCCCGTTGGA TGGAGACGAT GGTGATGACG AGGGCAACTT GAGCAAAGCA
    GAACGGCTGA GGGCGTGGAT CCGAACCCGG CTCCCTGTCT GCTGCCTGGA GCGAGACTCT
    TGGTCTGCCT ACATCTTCCC TCCTCAGTCA AGGTTCCGCC TCCTGTGTCA CCGAATCATC
    ACCCACAAGA TGTTTGACCA CGTGGTCCTC GTCATCATCT TCCTGAACTG TATCACCATC
    GCCATGGAGC GCCCCAAGAT CGACCCCCAC AGCGCTGAAC GAATTTTCCT GACCCTCTCC
    AATTACATCT TCACCGCAGT CTTTCTGGCT GAGATGACAG TGAAGGTGGT GGCGCTGGGC
    TGGTGTTTTG GGGAGCAGGC CTACCTGCGT AGCAGCTGGA ACGTGCTGGA CGGGCTGCTG
    GTGCTCATCT CCATCATCGA CATCCTGGTG TCCATGGTCT CCGACAGCGG CACCAAGATC
    CTGGGCATGC TGAGGGTACT GCGGCTGCTG CGGACCCTGC GCCCGCTCAG GGTAATCAGC
    CGGGCACAGG GGCTGAAGCT GGTGGTGGAA ACGCTGATGT CCTCGCTGAA GCCCATTGGC
    AACATTGTGG TCATCTGTTG TGCCTTCTTC ATCATATTTG GCATCCTAGG GGTGCAGCTC
    TTCAAAGGGA AGTTCTTTGT GTGCCAGGGC GAGGACACCA GGAACATCAC GAACAAGTCT
    GACTGTGCTG AGGCCAGTTA CCGGTGGGTC CGGCACAAAT ACAACTTTGA CAACCTCGGC
    CAGGCCCTAA TGTCCCTGTT CGTGCTGGCC TCTAAGGATG GCTGGGTGGA TATCATGTAT
    GATGGGCTGG ATGCTGTCGG CGTGGACCAG CAGCCCATCA TGAACCACAA CCCCTGGATG
    CTGCTGTACT TCATCTCGTT CCTGCTCATC GTGGCCTTCT TTGTCCTGAA CATGTTCGTT
    GGTGTGGTGG TGGAGAACTT CCACAAGTGT CGGCAGCACC AGGAGGAAGA GGAGGCCCGG
    CGGCGGGAGG AGAAGCGTCT ACGGAGACTG GAGAAAAAGA GAAGGAATCT AATGCTGGAC
    GATGTAATTG CTTCCGGCAG CTCAGCCAGC GCGGCGCCAG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC
    ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG
    GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC
    CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835403.1
    CDS152..6901
    Translation

    Target ORF information:

    RefSeq Version XM_024979635.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGTGGTGA AGATGGTGGC CTTGGGCATC TTTGGAAAAA AATGTTACCT GGGAGACACT 
    TGGAACCGGC TTGACTTTTT CATCGTCATT GCAGGGATGC TGGAGTATTC GCTGGACCTG
    CAGAATGTCA GCTTCTCAGC TGTCAGGACA GTCCGTGTGC TTCGACCCCT CAGGGCCATT
    AACCGGGTGC CCAGCATGCG CATCCTCGTC ACGCTGCTGC TGGACACGCT GCCCATGCTG
    GGCAACGTCC TGCTGCTCTG CTTCTTCGTC TTCTTCATCT TCGGCATCGT TGGTGTCCAG
    CTGTGGGCAG GACTGCTTCG TAACCGATGC TTCCTGCCTG AGAATTTCAG CCTCCCCCTG
    AGCGTGGACT TGGAGCGCTA CTACCAGACT GAGAACGAGG ATGAGAACCC ATTCATCTGC
    TCCCAGCCTC GTGAAAACGG CATGCGCTCC TGCAGGAGTG TGCCCACGCT GCGCGGGGAC
    GGGGGTGGCG GCCCACCCTG CGGCCTGGAC TACGAGGCCT ACAACAGCTC CAGCAACACC
    ACCTGTGTGA ACTGGAACCA GTACTACACC AACTGCTCCG CGGGGGAGCA CAACCCCTTC
    AAGGGTGCCA TCAACTTCGA CAACATTGGC TATGCCTGGA TCGCCATCTT CCAGGTCATC
    ACGCTGGAGG GCTGGGTCGA CATCATGTAC TTTGTGATGG ATGCTCACTC CTTCTACAAC
    TTCATCTACT TCATCCTTCT CATCATTGTG GGCTCTTTCT TTATGATCAA CCTGTGCCTG
    GTGGTGATTG CCACGCAGTT CTCAGAGACC AAGCAGCGGG AGAGCCAGCT GATGCGGGAG
    CAGCGCGTGA GATTCCTTTC CAACGCCAGC ACCCTGGCCA GCTTCTCCGA GCCCGGCAGC
    TGCTATGAGG AGCTGCTCAA GTACCTCGTG TATATCCTCC GGAAAGCAGC CCGAAGGCTG
    GCCCAGGTCT CCCGGGCGGC GGGCGTGCGG GTTGGCCTGC TGAGCAGCCC AGCGCCCCGT
    GGGGGCCAGG AGCCCCAGCC CAGTGGCGGC TGCCCTCGCC CCCACCGCCG TCCATCCGTC
    CACCATCTGG TGCACCACCA CCACCACCAC CACTACCACT TGGGCAATGG GACTCTGCGG
    GCCCCTCGGC CCAGCCCGCA GATCCAGGAC AGAGATGCCA GTGGGTCCCG CCGGCTCGTG
    CTGCCACCAC CCTCAACTCC TGCCCTTTCC AGGGGCCCTC CAGGGGGTGC AGAGGCTGTG
    CACAGCTTCT ACCATGCTGA TTGCCACTTG GAGCCAGTCC GCTGCCAGGC ACCCCCTCCC
    AGGTCGCCAT CGGAGGCATC GGGCAGGACC GTGGACAGTG GGAAGGTGTA CCCCACCGTG
    CACACCAGCC CTCCACCTGA GATGCTCAAA GAGAAGGCAC TAATGGAGGT GGCGCCCGCC
    TCTGGACCCA CCACCCTCAC CAGCCTCAAC ATCCCGCCCA GGCCCTACAG CTCTATGCAC
    AAGCTGCTGG AGACCCAGAG CACAGGCGCC TGCCAAAGCT CTTGCAAGAT CTCCAGCCCT
    TGCTTGAGGG CAGCCAGCGG AGCCTGTGGC CCAGACAGCT GTCCCTACTG TGCCCGGACC
    GGGGCAGGGG AGGTGGGGCT CACTGGCCAC GAGATGCCTG ACTCAGACAG TGAGGCGGTT
    TATGAGTTCA CACAAGATGC TCAGCATGGC GACCTCCGGG ATCCCCACAG TCGGCGTCGA
    CGGAGCCTGG GGCTGGATGT GGAGCCCAAC TCTGTGCTGG CCTTCTGGAG GCTGATCTGT
    GACACCTTCC GGAAGATTGT GGACAGCAAG TACTTTGGTC GGGGAATCAT GATTGCTATT
    CTGGTCAACA CGCTCAGCAT GGGCATCGAA TACCACGAGC AGCCTGAGGA GCTCACCAAT
    GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTTG CCCTGGAGAT GCTGCTGAAG
    CTGCTAGTAT ATGGTCCCTT TGGCTACATT AAGAACCCCT ACAACATCTT TGATGGCGTC
    ATTGTTGTCA TCAGCGTGTG GGAGATCGTG GGCCAGCAGG GGGGCGGCCT GTCGGTGCTG
    CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTGGTGCGCT TCTTGCCGGC GCTGCAGCGG
    CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG
    CTCTTCATCT TCATCTTCAG CATCCTGGGT ATGCATCTCT TCGGCTGCAA ATTTGCATCT
    GAGCGGGATG GGGACACGTT GCCGGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATC
    GTCACTGTCT TTCAGATCCT GACTCAGGAG GACTGGAACA AGGTGCTCTA CAACGGAATG
    GCCTCCACGT CATCCTGGGC TGCCCTTTAC TTCATCGCCC TCATGACCTT CGGCAACTAT
    GTGCTCTTCA ACCTGCTCGT TGCCATTCTG GTGGAGGGCT TCCAAACGGA GGAAATCAGC
    AAACGGGAAG ATGCGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCGA CTCCCAGGGG
    GGAGATGCCA CCAAGTCAGA GTCGGAACCT GATTTCTTCT CGCCCAGCCT GGATGGTGAT
    GGGGACAGGA AGAAGCGCTT GGCCTTGGTG TCTCTGGGAG AGCACCCGGA GCTGCGGAGG
    AGCTTGTTAC CACCGCTCAT CATCCACACG GCTGCCACTC CCATGTCATT GCCCAAAAGC
    TCCAGCAGCG GCCTGGCCGA GGTTCTGGGC CCCCCCTCCC GCCGCACCAG CAGCAGTGGG
    TCGGCTGAGC CAGGAGCACC TCACGAGATG AAGTCACCGC CAAGTGTCCG CAGTTCCCCC
    CACAGCCCCT GGAGTGCAGC AAGCAGCTGG ACGAGCAGGC GCTCCAGCAG GAACAGCCTG
    GGCCGTGCCC CCAGCCTGAA GCGAAGGAGC CCAAGTGGGG AGCGGAGGTC CTTGTTGTCG
    GGTGAGGGCC GGGAGAGCCA GGATGAGGAG GAGAGCTCGG AAGAGGAGCG CACTAGCCCA
    GCGGGCAGTG ACCGCCACCA CAGGGGCTCC CTGGAGAGGG AAGCTAAGAG CTCCTTTGAC
    CTACCGGACA CACTGCAGGT TCCCGGGCTG TACCGCACAG CCAGCGGCCG CAGCTCAGCC
    TCTGAGCACC AGGACTGCAA TGGCAATTCG GCCGTGGGAC GCCTGAGTCA GGCTGTGCGG
    CCCGATGACC CCCCGTTGGA TGGAGACGAT GGTGATGACG AGGGCAACTT GAGCAAAGCA
    GAACGGCTGA GGGCGTGGAT CCGAACCCGG CTCCCTGTCT GCTGCCTGGA GCGAGACTCT
    TGGTCTGCCT ACATCTTCCC TCCTCAGTCA AGGTTCCGCC TCCTGTGTCA CCGAATCATC
    ACCCACAAGA TGTTTGACCA CGTGGTCCTC GTCATCATCT TCCTGAACTG TATCACCATC
    GCCATGGAGC GCCCCAAGAT CGACCCCCAC AGCGCTGAAC GAATTTTCCT GACCCTCTCC
    AATTACATCT TCACCGCAGT CTTTCTGGCT GAGATGACAG TGAAGGTGGT GGCGCTGGGC
    TGGTGTTTTG GGGAGCAGGC CTACCTGCGT AGCAGCTGGA ACGTGCTGGA CGGGCTGCTG
    GTGCTCATCT CCATCATCGA CATCCTGGTG TCCATGGTCT CCGACAGCGG CACCAAGATC
    CTGGGCATGC TGAGGGTACT GCGGCTGCTG CGGACCCTGC GCCCGCTCAG GGTAATCAGC
    CGGGCACAGG GGCTGAAGCT GGTGGTGGAA ACGCTGATGT CCTCGCTGAA GCCCATTGGC
    AACATTGTGG TCATCTGTTG TGCCTTCTTC ATCATATTTG GCATCCTAGG GGTGCAGCTC
    TTCAAAGGGA AGTTCTTTGT GTGCCAGGGC GAGGACACCA GGAACATCAC GAACAAGTCT
    GACTGTGCTG AGGCCAGTTA CCGGTGGGTC CGGCACAAAT ACAACTTTGA CAACCTCGGC
    CAGGCCCTAA TGTCCCTGTT CGTGCTGGCC TCTAAGGATG GCTGGGTGGA TATCATGTAT
    GATGGGCTGG ATGCTGTCGG CGTGGACCAG CAGCCCATCA TGAACCACAA CCCCTGGATG
    CTGCTGTACT TCATCTCGTT CCTGCTCATC GTGGCCTTCT TTGTCCTGAA CATGTTCGTT
    GGTGTGGTGG TGGAGAACTT CCACAAGTGT CGGCAGCACC AGGAGGAAGA GGAGGCCCGG
    CGGCGGGAGG AGAAGCGTCT ACGGAGACTG GAGAAAAAGA GAAGGAATCT AATGCTGGAC
    GATGTAATTG CTTCCGGCAG CTCAGCCAGC GCGGCGCCAG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC
    ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG
    GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC
    CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226004
    Clone ID Related Accession (Same CDS sequence) XM_005220507.4
    Accession Version XM_005220507.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC
    GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG
    AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG
    AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG
    GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG
    CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC
    AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG
    AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG
    GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG
    CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC
    ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC
    TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC
    ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC
    AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG
    GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC
    TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC
    CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG
    GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220564.1
    CDS768..7508
    Translation

    Target ORF information:

    RefSeq Version XM_005220507.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220507.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC
    GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG
    AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG
    AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG
    GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG
    CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC
    AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG
    AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG
    GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG
    CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC
    ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC
    TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC
    ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC
    AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG
    GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC
    TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC
    CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG
    GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93902
    Clone ID Related Accession (Same CDS sequence) XM_015458770.1
    Accession Version XM_015458770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG
    GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT
    GCCTGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT
    TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG
    CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT
    GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC
    CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG
    TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC
    CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG
    CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC
    TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC
    ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG
    CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC
    AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC
    AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC
    TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC
    CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG
    GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC
    CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC
    AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC
    CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC
    AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA
    GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314256.1
    CDS731..7804
    Translation

    Target ORF information:

    RefSeq Version XM_015458770.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG
    GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT
    GCCTGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT
    TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG
    CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT
    GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC
    CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG
    TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC
    CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG
    CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC
    TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC
    ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG
    CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC
    AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC
    AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC
    TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC
    CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG
    GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC
    CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC
    AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC
    CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC
    AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA
    GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93903
    Clone ID Related Accession (Same CDS sequence) XM_015458771.1
    Accession Version XM_015458771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT
    GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC
    ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC
    ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG
    CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT AGGACACAGG
    GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA
    GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT
    GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG
    AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC
    AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC
    TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC
    TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC
    CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC
    CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC
    AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG
    CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC
    TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC
    CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC
    AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC
    TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC
    TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314257.1
    CDS731..7729
    Translation

    Target ORF information:

    RefSeq Version XM_015458771.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT
    GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC
    ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC
    ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG
    CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT AGGACACAGG
    GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA
    GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT
    GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG
    AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC
    AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC
    TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC
    TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC
    CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC
    CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC
    AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG
    CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC
    TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC
    CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC
    AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC
    TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC
    TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40889
    Clone ID Related Accession (Same CDS sequence) NM_001193140.1
    Accession Version NM_001193140.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03062363.1, AAFC03121466.1 and AAFC03062365.1. On Jul 17, 2010 this sequence version replaced XM_869265.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC
    CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC
    TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG
    GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT
    GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG
    TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT
    GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT
    GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC
    TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG
    CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA
    CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT
    TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC
    TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG
    CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC
    CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG
    GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT
    GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA
    TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC
    CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG
    AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG
    AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC
    AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC
    CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180069.1
    CDS1..7143
    Translation

    Target ORF information:

    RefSeq Version NM_001193140.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193140.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC
    CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC
    TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG
    GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT
    GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG
    TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT
    GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT
    GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC
    TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG
    CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA
    CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT
    TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC
    TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG
    CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC
    CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG
    GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT
    GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA
    TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC
    CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG
    AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG
    AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC
    AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC
    CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29847
    Clone ID Related Accession (Same CDS sequence) XM_005220487.3
    Accession Version XM_005220487.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC
    ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG
    GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCT GCAGCCCAGG CGGCTCCGAC
    CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220544.1
    CDS731..7840
    Translation

    Target ORF information:

    RefSeq Version XM_005220487.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220487.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC
    ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG
    GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCT GCAGCCCAGG CGGCTCCGAC
    CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29848
    Clone ID Related Accession (Same CDS sequence) XM_005220488.3
    Accession Version XM_005220488.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC
    CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA
    GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC TGCAGCCCAG GCGGCTCCGA CCCACAGATC
    CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC
    TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC
    CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220545.1
    CDS731..7771
    Translation

    Target ORF information:

    RefSeq Version XM_005220488.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220488.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC
    CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA
    GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC TGCAGCCCAG GCGGCTCCGA CCCACAGATC
    CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC
    TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC
    CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29854
    Clone ID Related Accession (Same CDS sequence) XM_005220494.3
    Accession Version XM_005220494.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220551.1
    CDS731..7660
    Translation

    Target ORF information:

    RefSeq Version XM_005220494.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220494.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA
    CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA
    CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29857
    Clone ID Related Accession (Same CDS sequence) XM_005220497.3
    Accession Version XM_005220497.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT
    CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC
    ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC
    AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC
    CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC
    ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC
    CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG
    CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC
    TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC
    CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA
    AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC
    AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG
    AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC
    AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT
    CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC
    ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC
    CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC
    AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT
    GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220554.1
    CDS730..7593
    Translation

    Target ORF information:

    RefSeq Version XM_005220497.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC
    AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC
    CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG
    CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT
    CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG
    GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT
    GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC
    ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA
    CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG
    GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG
    GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT
    GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC
    AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC
    AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG
    GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG
    GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC
    CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC
    AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC
    CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT
    CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC
    ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC
    AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC
    CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC
    ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC
    CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG
    CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC
    TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC
    CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA
    AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC
    AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG
    AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC
    AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT
    CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC
    ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC
    CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC
    AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT
    GACCCAGCAG ACCTGGACCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29859
    Clone ID Related Accession (Same CDS sequence) XM_005220499.3
    Accession Version XM_005220499.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG
    GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA
    ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC
    CTAGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC
    TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC
    CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC
    CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT
    CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG
    GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC
    CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG
    TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG
    ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG
    AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT
    GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG
    GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG
    AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT
    CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG
    GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC
    GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220556.1
    CDS730..7560
    Translation

    Target ORF information:

    RefSeq Version XM_005220499.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220499.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC
    TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG
    GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC
    CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC
    TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG
    GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG
    CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG
    GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG
    TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG
    CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG
    ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG
    GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC
    TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT
    CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA
    GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT
    GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC
    CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG
    GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA
    ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC
    CTAGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC
    TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC
    CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC
    CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT
    CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG
    GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC
    CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG
    TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG
    ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG
    AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT
    GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG
    GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG
    AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT
    CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG
    GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC
    GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29861
    Clone ID Related Accession (Same CDS sequence) XM_005220501.3
    Accession Version XM_005220501.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220558.1
    CDS730..7539
    Translation

    Target ORF information:

    RefSeq Version XM_005220501.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220501.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA
    AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA
    CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG
    GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC
    ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG
    GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG
    ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC
    TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG
    AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG
    GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC
    TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG
    CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT
    TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC
    GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC
    CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG
    TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC
    CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC
    CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG
    GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG
    CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG
    GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG
    CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG
    GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC
    TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG
    GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG
    GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG
    GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC
    CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC
    ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG
    TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA
    CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC
    CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC
    ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT
    GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC
    AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG
    GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG
    CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA
    AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG
    CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG
    GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC
    CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA
    GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC
    TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT
    GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG
    GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA
    CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC
    CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC
    TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA
    CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC
    AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG
    CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC
    TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC
    TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC
    AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA
    GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG
    GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC
    ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG
    ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC
    ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG
    GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC
    GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT
    GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC
    ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT
    GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT
    CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA
    TCCTCTGACC CAGCAGACCT GGACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29862
    Clone ID Related Accession (Same CDS sequence) XM_005220502.3
    Accession Version XM_005220502.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT
    GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG
    GATGGGAGCA CTGCCGAGGG GTCCCTAGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG
    TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT
    CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC
    AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA
    ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG
    GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC
    CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC
    CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA
    GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG
    GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG
    CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG
    GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA
    GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC
    CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG
    AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT
    GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA
    GACCTGGACC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220559.1
    CDS730..7524
    Translation

    Target ORF information:

    RefSeq Version XM_005220502.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220502.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG
    CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC
    CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG
    GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC
    TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT
    GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG
    TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC
    ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG
    GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC
    TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT
    GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT
    CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT
    GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC
    ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG
    GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC
    GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA
    CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA
    CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC
    CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT
    GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG
    GATGGGAGCA CTGCCGAGGG GTCCCTAGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG
    TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT
    CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC
    AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA
    ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG
    GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC
    CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC
    CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA
    GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG
    GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG
    CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG
    GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA
    GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC
    CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG
    AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT
    GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA
    GACCTGGACC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29865
    Clone ID Related Accession (Same CDS sequence) XM_005220505.3
    Accession Version XM_005220505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC
    GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT
    CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTAGGACAC
    AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA
    GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT
    GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT
    CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG
    GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC
    TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG
    ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG
    GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA
    GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC
    TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG
    AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT
    CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC
    AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA
    CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG
    GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT
    CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220562.1
    CDS730..7491
    Translation

    Target ORF information:

    RefSeq Version XM_005220505.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC
    TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC
    ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC
    CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG
    GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC
    CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG
    GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT
    GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG
    ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC
    TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC
    GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC
    CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC
    CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG
    ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG
    AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG
    ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG
    GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA
    GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC
    GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT
    CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTAGGACAC
    AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA
    GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT
    GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT
    CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG
    GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC
    TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG
    ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG
    GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA
    GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC
    TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG
    AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT
    CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC
    AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA
    CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG
    GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT
    CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29867
    Clone ID Related Accession (Same CDS sequence) XM_005220507.3
    Accession Version XM_005220507.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC
    GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG
    AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG
    AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG
    GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG
    CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC
    AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG
    AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG
    GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG
    CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC
    ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC
    TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC
    ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC
    AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG
    GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC
    TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC
    CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG
    GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220564.1
    CDS730..7470
    Translation

    Target ORF information:

    RefSeq Version XM_005220507.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220507.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
    CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC
    CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT
    TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC
    CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG
    TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT
    GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC
    ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC
    ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG
    ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC
    GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC
    GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA
    TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG
    ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC
    TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG
    GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC
    ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT
    GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG
    TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT
    GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG
    ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC
    AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC
    GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG
    CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC
    GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC
    CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG
    GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT
    TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC
    TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG
    ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC
    AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC
    AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC
    GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT
    GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC
    CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT
    GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC
    AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC
    AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC
    GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC
    ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC
    ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC
    GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG
    AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG
    GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG
    GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC
    CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG
    GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT
    TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT
    CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC
    TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG
    GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC
    ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC
    TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA
    CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC
    AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT
    GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC
    TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG
    AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC
    ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG
    GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT
    GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT
    GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC
    CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC
    ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT
    GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG
    ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC
    TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG
    GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC
    CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG
    ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA
    TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC
    ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC
    AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG
    GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC
    ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC
    TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG
    CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA
    AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC
    GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG
    AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG
    AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG
    GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG
    CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC
    AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG
    AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG
    GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG
    CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC
    ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC
    TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC
    ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC
    AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG
    GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC
    TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC
    CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG
    GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG
    CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG
    TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA
    GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG
    CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC
    ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG
    GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG
    TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC
    AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG
    TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC
    AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT
    CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC
    TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC
    TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG
    CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA
    GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC
    CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC
    ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC
    CCAGCAGACC TGGACCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.