CACNA1G gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005220488.3	XP_005220545.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_005220499.3	XP_005220556.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_005220501.3	XP_005220558.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_015458772.1	XP_015314258.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
NM_001193140.1	NP_001180069.1	voltage-dependent T-type calcium channel subunit alpha-1G
XM_015458770.2	XP_015314256.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_005220505.4	XP_005220562.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
XM_015458770.1	XP_015314256.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_005220494.3	XP_005220551.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
XM_005220502.3	XP_005220559.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
NM_001193140.3	NP_001180069.2	voltage-dependent T-type calcium channel subunit alpha-1G
XM_005220497.4	XP_005220554.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_005220502.4	XP_005220559.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
XM_024979635.1	XP_024835403.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
XM_010816118.2	XP_010814420.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_005220487.3	XP_005220544.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_005220505.3	XP_005220562.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
XM_005220507.3	XP_005220564.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
NM_001193140.2	NP_001180069.1	voltage-dependent T-type calcium channel subunit alpha-1G
XM_005220499.4	XP_005220556.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_005220507.4	XP_005220564.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
XM_015458771.1	XP_015314257.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_005220497.3	XP_005220554.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_024979634.1	XP_024835402.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
XM_005220494.4	XP_005220551.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_005220487.4	XP_005220544.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_015458771.2	XP_015314257.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_010816118.3	XP_010814420.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_005220488.4	XP_005220545.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_005220501.4	XP_005220558.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa45434	XM_010816118.2 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1371.30 $1959.00
OBa93904	XM_015458772.1 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa216272	NM_001193140.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa238994	NM_001193140.3 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa225991	XM_005220487.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa225992	XM_010816118.3 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa225993	XM_015458770.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa225994	XM_005220488.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa225995	XM_015458771.2 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa225996	XM_024979634.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa225997	XM_005220494.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa225998	XM_005220497.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa225999	XM_005220499.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa226000	XM_005220501.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa226001	XM_005220502.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa226002	XM_005220505.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa226003	XM_024979635.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa226004	XM_005220507.4 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa93902	XM_015458770.1 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa93903	XM_015458771.1 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa40889	NM_001193140.1 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa29847	XM_005220487.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa29848	XM_005220488.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa29854	XM_005220494.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa29857	XM_005220497.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa29859	XM_005220499.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa29861	XM_005220501.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa29862	XM_005220502.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa29865	XM_005220505.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa29867	XM_005220507.3 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

CACNA1G ( XM_010816118.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814420.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45434
Clone ID Related Accession (Same CDS sequence)	XM_010816118.2
Accession Version	XM_010816118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCTGCCTG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG 
ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT 
ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC 
AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG 
AGCACTGCCG AGGGGTCCCT AGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC 
TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA 
GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG 
CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG 
ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT 
GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT 
CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC 
CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC 
CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG 
CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT 
GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA 
GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG 
AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT 
CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG 
CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814420.1
CDS	731..7819
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPACSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_010816118.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816118.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCTGCCTG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG 
ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT 
ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC 
AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG 
AGCACTGCCG AGGGGTCCCT AGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC 
TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA 
GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG 
CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG 
ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT 
GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT 
CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC 
CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC 
CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG 
CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT 
GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA 
GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG 
AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT 
CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG 
CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_015458772.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314258.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.

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CloneID	OBa93904
Clone ID Related Accession (Same CDS sequence)	XM_015458772.1
Accession Version	XM_015458772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG 
ACCCTCCTGC CTCGATGTGG AGAGGTAAGC AGGCCCTTGG CTGTCCCCAG AGATGGCTGC 
CCACACTTGC AGCGTGGGGC CACCCCAGCA AGCCACTGGC ACACTTGCTG GCTCATCTGT 
CCCGGTGCCT CCAGCTTGGC TGGGACTTCC CTTCCTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314258.1
CDS	734..6718
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMGAGSKQTLGLLLNHLTLLPRCGEVSRPLAVPRDGCPHLQRGATPASHWHTCWLICPGASSLAGTSLPGF

Target ORF information:

RefSeq Version	XM_015458772.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458772.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG 
ACCCTCCTGC CTCGATGTGG AGAGGTAAGC AGGCCCTTGG CTGTCCCCAG AGATGGCTGC 
CCACACTTGC AGCGTGGGGC CACCCCAGCA AGCCACTGGC ACACTTGCTG GCTCATCTGT 
CCCGGTGCCT CCAGCTTGGC TGGGACTTCC CTTCCTGGCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( NM_001193140.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180069.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.

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CloneID	OBa216272
Clone ID Related Accession (Same CDS sequence)	NM_001193140.2
Accession Version	NM_001193140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02049035.1, DAAA02049034.1 and DAAA02049033.1. On Jun 30, 2017 this sequence version replaced NM_001193140.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC 
CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC 
TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG 
GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT 
GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG 
TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT 
GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT 
GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC 
TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG 
CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA 
CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT 
TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC 
TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG 
CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC 
CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT 
GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA 
TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC 
CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG 
AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG 
AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC 
AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180069.1
CDS	1..7143
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPACSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	NM_001193140.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193140.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC 
CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC 
TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG 
GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT 
GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG 
TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT 
GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT 
GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC 
TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG 
CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA 
CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT 
TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC 
TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG 
CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC 
CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT 
GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA 
TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC 
CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG 
AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG 
AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC 
AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( NM_001193140.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180069.2 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.

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CloneID	OBa238994
Clone ID Related Accession (Same CDS sequence)	NM_001193140.3
Accession Version	NM_001193140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On May 29, 2018 this sequence version replaced NM_001193140.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC 
CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC 
TTCCCTCCTG CCCGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG 
GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT 
GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG 
TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT 
GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT 
GCCGAGGGGT CCCTGGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC 
TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG 
CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA 
CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT 
TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC 
TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG 
CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC 
CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT 
GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA 
TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC 
CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG 
AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG 
AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC 
AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180069.2
CDS	1..7143
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPARSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	NM_001193140.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193140.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC 
CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC 
TTCCCTCCTG CCCGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG 
GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT 
GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG 
TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT 
GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT 
GCCGAGGGGT CCCTGGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC 
TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG 
CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA 
CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT 
TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC 
TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG 
CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC 
CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT 
GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA 
TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC 
CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG 
AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG 
AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC 
AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220487.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220544.2 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.

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CloneID	OBa225991
Clone ID Related Accession (Same CDS sequence)	XM_005220487.4
Accession Version	XM_005220487.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220487.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220544.2
CDS	1115..8224
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPARSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220487.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220487.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_010816118.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814420.2 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.

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CloneID	OBa225992
Clone ID Related Accession (Same CDS sequence)	XM_010816118.3
Accession Version	XM_010816118.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCTGCCCG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG 
ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT 
ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC 
AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG 
AGCACTGCCG AGGGGTCCCT GGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC 
TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA 
GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG 
CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG 
ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT 
GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT 
CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC 
CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC 
CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG 
CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT 
GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA 
GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG 
AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT 
CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG 
CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814420.2
CDS	1115..8203
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPARSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_010816118.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816118.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GGACAGACAG CTGTTTGACA CCATATCCCT GCTGATCCAG 
GGCTCCCTGG ATGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTCCTGCCCG CAGCCCAGGC GGCTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTTTGAC GTCAGAGATT GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG 
ACGGATGACT GTCTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGCGAGCAAT 
ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC ATGCTAGGAC CAGATCTGCT GACTGTGCGC 
AAGTCTGGGG TCAGCCGAAC GCACTCTCTG CCCAATGACA GCTACATGTG CCGGGATGGG 
AGCACTGCCG AGGGGTCCCT GGGACACAGG GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC 
TCCATCTTGT CCGTCCACTC CCAGCCAGCA GACACCAGCT ACATCCCGCA GCTTCCCAAA 
GATGCGCCCC ATCTGCTCCA GCCCCATGGT GCTCCCACCT GGGGCACCAT CCCCAAATTG 
CCCCCACCAG GCCGCTCCCC TTTGGCTCAG AGGCCACTCA GGCGCCAGGC AGCAATACGG 
ACGGATTCCC TGGACATTCA GGGCCTGGGC AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT 
GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC TCCTTTTGGG GCCACTCCAG CACCCAGGCT 
CAGCAGCATT CCCGCAGCCA GAGCAAGATC TTGAAGCACA TGCCCCCGTC AGCCCCCTGC 
CCAGGCCCAG AACTCAAGTG GGCCAAGGGC CCACCAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA CGGACAGTCA GGAGGAAGCC 
CCGTCTGCAC GGGACCTGAA GAAGTGCTAC AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG 
CCGGCATCCT GGCTGGATGA ACAGAGGAGG CACTCCATCG CGGTCAGCTG CCTGGACAGT 
GGCTCCCAAC CCGCCCTGGG CCCTGATCCC TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA 
GCTGGGAGCC GGCCCAAGAA GAAACTCAGC CCACCCAGCA TCTCCATAGA CCCCCCAGAG 
AGCCAGAGCC AGGGCCCTCG GCCCCCACCC AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT 
CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC TCTGGCCCTC CTGACAGCAT GGCTGCCTCG 
CCCTCCCCAA AGAAAGACGT GCTGAGTCTC TCTGGTTTAT CCTCTGACCC AGCAGACCTG 
GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_015458770.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314256.2 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.

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CloneID	OBa225993
Clone ID Related Accession (Same CDS sequence)	XM_015458770.2
Accession Version	XM_015458770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT 
GCCCGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT 
GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC 
CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG 
TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC 
CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG 
CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC 
TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC 
ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG 
CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC 
AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC 
AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC 
CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG 
GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC 
CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC 
AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC 
CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC 
AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314256.2
CDS	1115..8188
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPARSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_015458770.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458770.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT 
GCCCGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT 
GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC 
CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG 
TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC 
CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG 
CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC 
TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC 
ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG 
CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC 
AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC 
AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC 
CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG 
GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC 
CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC 
AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC 
CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC 
AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220488.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220545.2 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.

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CloneID	OBa225994
Clone ID Related Accession (Same CDS sequence)	XM_005220488.4
Accession Version	XM_005220488.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220488.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC 
CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC CGCAGCCCAG GCGGCTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC 
TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220545.2
CDS	772..7812
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPARSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220488.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220488.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC 
CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC CGCAGCCCAG GCGGCTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC 
TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_015458771.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314257.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.

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CloneID	OBa225995
Clone ID Related Accession (Same CDS sequence)	XM_015458771.2
Accession Version	XM_015458771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT 
GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC 
ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG 
CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT GGGACACAGG 
GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA 
GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT 
GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC 
AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC 
TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC 
TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC 
CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC 
AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG 
CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC 
TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC 
CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC 
AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC 
TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC 
TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314257.1
CDS	772..7770
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_015458771.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458771.2

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT 
GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC 
ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG 
CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT GGGACACAGG 
GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA 
GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT 
GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC 
AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC 
TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC 
TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC 
CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC 
AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG 
CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC 
TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC 
CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC 
AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC 
TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC 
TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_024979634.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835402.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.

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CloneID	OBa225996
Clone ID Related Accession (Same CDS sequence)	XM_024979634.1
Accession Version	XM_024979634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG 
ACCCTCCTGC CTCGATGTGG AGAGATGGAG TCCCACCCTG AGGAGGTGCC CGTCATGCTA 
GGACCAGATC TGCTGACTGT GCGCAAGTCT GGGGTCAGCC GAACGCACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGGGA TGGGAGCACT GCCGAGGGGT CCCTGGGACA CAGGGGCTGG 
GGGCTCCCCA GAGCTCAGTC AGGCTCCATC TTGTCCGTCC ACTCCCAGCC AGCAGACACC 
AGCTACATCC CGCAGCTTCC CAAAGATGCG CCCCATCTGC TCCAGCCCCA TGGTGCTCCC 
ACCTGGGGCA CCATCCCCAA ATTGCCCCCA CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT ACGGACGGAT TCCCTGGACA TTCAGGGCCT GGGCAGCAGG 
GAAGACCTGC TGTCAGAGGT GAGTGGGCCC TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT 
TGGGGCCACT CCAGCACCCA GGCTCAGCAG CATTCCCGCA GCCAGAGCAA GATCTTGAAG 
CACATGCCCC CGTCAGCCCC CTGCCCAGGC CCAGAACTCA AGTGGGCCAA GGGCCCACCA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCACGGACA GTCAGGAGGA AGCCCCGTCT GCACGGGACC TGAAGAAGTG CTACAGCGTG 
GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA TCCTGGCTGG ATGAACAGAG GAGGCACTCC 
ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC CAACCCGCCC TGGGCCCTGA TCCCTCGAGC 
CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG AGCCGGCCCA AGAAGAAACT CAGCCCACCC 
AGCATCTCCA TAGACCCCCC AGAGAGCCAG AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT 
GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC AGCGACTCCA AGGATCCCTT GGCCTCTGGC 
CCTCCTGACA GCATGGCTGC CTCGCCCTCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTATCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835402.1
CDS	771..7703
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMGAGSKQTLGLLLNHLTLLPRCGEMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_024979634.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979634.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGGAGC AGGCAGCAAG CAGACCCTAG GGCTCCTCCT GAATCACCTG 
ACCCTCCTGC CTCGATGTGG AGAGATGGAG TCCCACCCTG AGGAGGTGCC CGTCATGCTA 
GGACCAGATC TGCTGACTGT GCGCAAGTCT GGGGTCAGCC GAACGCACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGGGA TGGGAGCACT GCCGAGGGGT CCCTGGGACA CAGGGGCTGG 
GGGCTCCCCA GAGCTCAGTC AGGCTCCATC TTGTCCGTCC ACTCCCAGCC AGCAGACACC 
AGCTACATCC CGCAGCTTCC CAAAGATGCG CCCCATCTGC TCCAGCCCCA TGGTGCTCCC 
ACCTGGGGCA CCATCCCCAA ATTGCCCCCA CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA 
CTCAGGCGCC AGGCAGCAAT ACGGACGGAT TCCCTGGACA TTCAGGGCCT GGGCAGCAGG 
GAAGACCTGC TGTCAGAGGT GAGTGGGCCC TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT 
TGGGGCCACT CCAGCACCCA GGCTCAGCAG CATTCCCGCA GCCAGAGCAA GATCTTGAAG 
CACATGCCCC CGTCAGCCCC CTGCCCAGGC CCAGAACTCA AGTGGGCCAA GGGCCCACCA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCACGGACA GTCAGGAGGA AGCCCCGTCT GCACGGGACC TGAAGAAGTG CTACAGCGTG 
GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA TCCTGGCTGG ATGAACAGAG GAGGCACTCC 
ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC CAACCCGCCC TGGGCCCTGA TCCCTCGAGC 
CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG AGCCGGCCCA AGAAGAAACT CAGCCCACCC 
AGCATCTCCA TAGACCCCCC AGAGAGCCAG AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT 
GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC AGCGACTCCA AGGATCCCTT GGCCTCTGGC 
CCTCCTGACA GCATGGCTGC CTCGCCCTCC CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT 
TTATCCTCTG ACCCAGCAGA CCTGGACCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220494.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220551.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.

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CloneID	OBa225997
Clone ID Related Accession (Same CDS sequence)	XM_005220494.4
Accession Version	XM_005220494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220551.1
CDS	771..7700
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220494.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220494.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220497.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220554.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.

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CloneID	OBa225998
Clone ID Related Accession (Same CDS sequence)	XM_005220497.4
Accession Version	XM_005220497.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT 
CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC 
AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC 
CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC 
ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG 
CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC 
TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC 
CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC 
AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG 
AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC 
AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT 
CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC 
ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC 
CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC 
AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220554.1
CDS	769..7632
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220497.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220497.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT 
CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTGGGAC ACAGGGGCTG GGGGCTCCCC 
AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC 
CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC 
ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG 
CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC 
TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC 
CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC 
AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG 
AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC 
AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT 
CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC 
ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC 
CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC 
AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220499.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220556.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.

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CloneID	OBa225999
Clone ID Related Accession (Same CDS sequence)	XM_005220499.4
Accession Version	XM_005220499.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG 
GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA 
ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC 
CTGGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC 
TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC 
CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC 
CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT 
CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG 
GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC 
CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG 
TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG 
AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT 
GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG 
GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG 
AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT 
CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG 
GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220556.1
CDS	768..7598
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220499.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220499.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG 
GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA 
ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC 
CTGGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC 
TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC 
CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC 
CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT 
CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG 
GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC 
CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG 
TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG 
AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT 
GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG 
GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG 
AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT 
CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG 
GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220501.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220558.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.

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CloneID	OBa226000
Clone ID Related Accession (Same CDS sequence)	XM_005220501.4
Accession Version	XM_005220501.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220501.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220558.1
CDS	768..7577
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220501.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220501.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220502.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220559.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.

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CloneID	OBa226001
Clone ID Related Accession (Same CDS sequence)	XM_005220502.4
Accession Version	XM_005220502.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220502.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT 
GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG 
GATGGGAGCA CTGCCGAGGG GTCCCTGGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG 
TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT 
CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC 
AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG 
GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC 
CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC 
CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG 
GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG 
CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG 
GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA 
GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC 
CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG 
AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT 
GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220559.1
CDS	768..7562
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220502.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220502.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT 
GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG 
GATGGGAGCA CTGCCGAGGG GTCCCTGGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG 
TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT 
CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC 
AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG 
GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC 
CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC 
CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG 
GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG 
CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG 
GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA 
GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC 
CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG 
AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT 
GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220505.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220562.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.

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CloneID	OBa226002
Clone ID Related Accession (Same CDS sequence)	XM_005220505.4
Accession Version	XM_005220505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC 
GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTGGGACAC 
AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA 
GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT 
GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG 
GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC 
TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG 
ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG 
GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC 
TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG 
AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT 
CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC 
AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA 
CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG 
GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220562.1
CDS	768..7529
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220505.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220505.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC 
GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTGGGACAC 
AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA 
GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT 
GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG 
GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC 
TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG 
ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG 
GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC 
TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG 
AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT 
CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC 
AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA 
CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG 
GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_024979635.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835403.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.

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CloneID	OBa226003
Clone ID Related Accession (Same CDS sequence)	XM_024979635.1
Accession Version	XM_024979635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGA AGATGGTGGC CTTGGGCATC TTTGGAAAAA AATGTTACCT GGGAGACACT 
TGGAACCGGC TTGACTTTTT CATCGTCATT GCAGGGATGC TGGAGTATTC GCTGGACCTG 
CAGAATGTCA GCTTCTCAGC TGTCAGGACA GTCCGTGTGC TTCGACCCCT CAGGGCCATT 
AACCGGGTGC CCAGCATGCG CATCCTCGTC ACGCTGCTGC TGGACACGCT GCCCATGCTG 
GGCAACGTCC TGCTGCTCTG CTTCTTCGTC TTCTTCATCT TCGGCATCGT TGGTGTCCAG 
CTGTGGGCAG GACTGCTTCG TAACCGATGC TTCCTGCCTG AGAATTTCAG CCTCCCCCTG 
AGCGTGGACT TGGAGCGCTA CTACCAGACT GAGAACGAGG ATGAGAACCC ATTCATCTGC 
TCCCAGCCTC GTGAAAACGG CATGCGCTCC TGCAGGAGTG TGCCCACGCT GCGCGGGGAC 
GGGGGTGGCG GCCCACCCTG CGGCCTGGAC TACGAGGCCT ACAACAGCTC CAGCAACACC 
ACCTGTGTGA ACTGGAACCA GTACTACACC AACTGCTCCG CGGGGGAGCA CAACCCCTTC 
AAGGGTGCCA TCAACTTCGA CAACATTGGC TATGCCTGGA TCGCCATCTT CCAGGTCATC 
ACGCTGGAGG GCTGGGTCGA CATCATGTAC TTTGTGATGG ATGCTCACTC CTTCTACAAC 
TTCATCTACT TCATCCTTCT CATCATTGTG GGCTCTTTCT TTATGATCAA CCTGTGCCTG 
GTGGTGATTG CCACGCAGTT CTCAGAGACC AAGCAGCGGG AGAGCCAGCT GATGCGGGAG 
CAGCGCGTGA GATTCCTTTC CAACGCCAGC ACCCTGGCCA GCTTCTCCGA GCCCGGCAGC 
TGCTATGAGG AGCTGCTCAA GTACCTCGTG TATATCCTCC GGAAAGCAGC CCGAAGGCTG 
GCCCAGGTCT CCCGGGCGGC GGGCGTGCGG GTTGGCCTGC TGAGCAGCCC AGCGCCCCGT 
GGGGGCCAGG AGCCCCAGCC CAGTGGCGGC TGCCCTCGCC CCCACCGCCG TCCATCCGTC 
CACCATCTGG TGCACCACCA CCACCACCAC CACTACCACT TGGGCAATGG GACTCTGCGG 
GCCCCTCGGC CCAGCCCGCA GATCCAGGAC AGAGATGCCA GTGGGTCCCG CCGGCTCGTG 
CTGCCACCAC CCTCAACTCC TGCCCTTTCC AGGGGCCCTC CAGGGGGTGC AGAGGCTGTG 
CACAGCTTCT ACCATGCTGA TTGCCACTTG GAGCCAGTCC GCTGCCAGGC ACCCCCTCCC 
AGGTCGCCAT CGGAGGCATC GGGCAGGACC GTGGACAGTG GGAAGGTGTA CCCCACCGTG 
CACACCAGCC CTCCACCTGA GATGCTCAAA GAGAAGGCAC TAATGGAGGT GGCGCCCGCC 
TCTGGACCCA CCACCCTCAC CAGCCTCAAC ATCCCGCCCA GGCCCTACAG CTCTATGCAC 
AAGCTGCTGG AGACCCAGAG CACAGGCGCC TGCCAAAGCT CTTGCAAGAT CTCCAGCCCT 
TGCTTGAGGG CAGCCAGCGG AGCCTGTGGC CCAGACAGCT GTCCCTACTG TGCCCGGACC 
GGGGCAGGGG AGGTGGGGCT CACTGGCCAC GAGATGCCTG ACTCAGACAG TGAGGCGGTT 
TATGAGTTCA CACAAGATGC TCAGCATGGC GACCTCCGGG ATCCCCACAG TCGGCGTCGA 
CGGAGCCTGG GGCTGGATGT GGAGCCCAAC TCTGTGCTGG CCTTCTGGAG GCTGATCTGT 
GACACCTTCC GGAAGATTGT GGACAGCAAG TACTTTGGTC GGGGAATCAT GATTGCTATT 
CTGGTCAACA CGCTCAGCAT GGGCATCGAA TACCACGAGC AGCCTGAGGA GCTCACCAAT 
GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTTG CCCTGGAGAT GCTGCTGAAG 
CTGCTAGTAT ATGGTCCCTT TGGCTACATT AAGAACCCCT ACAACATCTT TGATGGCGTC 
ATTGTTGTCA TCAGCGTGTG GGAGATCGTG GGCCAGCAGG GGGGCGGCCT GTCGGTGCTG 
CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTGGTGCGCT TCTTGCCGGC GCTGCAGCGG 
CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGT ATGCATCTCT TCGGCTGCAA ATTTGCATCT 
GAGCGGGATG GGGACACGTT GCCGGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATC 
GTCACTGTCT TTCAGATCCT GACTCAGGAG GACTGGAACA AGGTGCTCTA CAACGGAATG 
GCCTCCACGT CATCCTGGGC TGCCCTTTAC TTCATCGCCC TCATGACCTT CGGCAACTAT 
GTGCTCTTCA ACCTGCTCGT TGCCATTCTG GTGGAGGGCT TCCAAACGGA GGAAATCAGC 
AAACGGGAAG ATGCGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCGA CTCCCAGGGG 
GGAGATGCCA CCAAGTCAGA GTCGGAACCT GATTTCTTCT CGCCCAGCCT GGATGGTGAT 
GGGGACAGGA AGAAGCGCTT GGCCTTGGTG TCTCTGGGAG AGCACCCGGA GCTGCGGAGG 
AGCTTGTTAC CACCGCTCAT CATCCACACG GCTGCCACTC CCATGTCATT GCCCAAAAGC 
TCCAGCAGCG GCCTGGCCGA GGTTCTGGGC CCCCCCTCCC GCCGCACCAG CAGCAGTGGG 
TCGGCTGAGC CAGGAGCACC TCACGAGATG AAGTCACCGC CAAGTGTCCG CAGTTCCCCC 
CACAGCCCCT GGAGTGCAGC AAGCAGCTGG ACGAGCAGGC GCTCCAGCAG GAACAGCCTG 
GGCCGTGCCC CCAGCCTGAA GCGAAGGAGC CCAAGTGGGG AGCGGAGGTC CTTGTTGTCG 
GGTGAGGGCC GGGAGAGCCA GGATGAGGAG GAGAGCTCGG AAGAGGAGCG CACTAGCCCA 
GCGGGCAGTG ACCGCCACCA CAGGGGCTCC CTGGAGAGGG AAGCTAAGAG CTCCTTTGAC 
CTACCGGACA CACTGCAGGT TCCCGGGCTG TACCGCACAG CCAGCGGCCG CAGCTCAGCC 
TCTGAGCACC AGGACTGCAA TGGCAATTCG GCCGTGGGAC GCCTGAGTCA GGCTGTGCGG 
CCCGATGACC CCCCGTTGGA TGGAGACGAT GGTGATGACG AGGGCAACTT GAGCAAAGCA 
GAACGGCTGA GGGCGTGGAT CCGAACCCGG CTCCCTGTCT GCTGCCTGGA GCGAGACTCT 
TGGTCTGCCT ACATCTTCCC TCCTCAGTCA AGGTTCCGCC TCCTGTGTCA CCGAATCATC 
ACCCACAAGA TGTTTGACCA CGTGGTCCTC GTCATCATCT TCCTGAACTG TATCACCATC 
GCCATGGAGC GCCCCAAGAT CGACCCCCAC AGCGCTGAAC GAATTTTCCT GACCCTCTCC 
AATTACATCT TCACCGCAGT CTTTCTGGCT GAGATGACAG TGAAGGTGGT GGCGCTGGGC 
TGGTGTTTTG GGGAGCAGGC CTACCTGCGT AGCAGCTGGA ACGTGCTGGA CGGGCTGCTG 
GTGCTCATCT CCATCATCGA CATCCTGGTG TCCATGGTCT CCGACAGCGG CACCAAGATC 
CTGGGCATGC TGAGGGTACT GCGGCTGCTG CGGACCCTGC GCCCGCTCAG GGTAATCAGC 
CGGGCACAGG GGCTGAAGCT GGTGGTGGAA ACGCTGATGT CCTCGCTGAA GCCCATTGGC 
AACATTGTGG TCATCTGTTG TGCCTTCTTC ATCATATTTG GCATCCTAGG GGTGCAGCTC 
TTCAAAGGGA AGTTCTTTGT GTGCCAGGGC GAGGACACCA GGAACATCAC GAACAAGTCT 
GACTGTGCTG AGGCCAGTTA CCGGTGGGTC CGGCACAAAT ACAACTTTGA CAACCTCGGC 
CAGGCCCTAA TGTCCCTGTT CGTGCTGGCC TCTAAGGATG GCTGGGTGGA TATCATGTAT 
GATGGGCTGG ATGCTGTCGG CGTGGACCAG CAGCCCATCA TGAACCACAA CCCCTGGATG 
CTGCTGTACT TCATCTCGTT CCTGCTCATC GTGGCCTTCT TTGTCCTGAA CATGTTCGTT 
GGTGTGGTGG TGGAGAACTT CCACAAGTGT CGGCAGCACC AGGAGGAAGA GGAGGCCCGG 
CGGCGGGAGG AGAAGCGTCT ACGGAGACTG GAGAAAAAGA GAAGGAATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCAGCCAGC GCGGCGCCAG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835403.1
CDS	152..6901
Translation	MVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPARSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_024979635.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979635.1

ORF Insert Sequence:

ATGGTGGTGA AGATGGTGGC CTTGGGCATC TTTGGAAAAA AATGTTACCT GGGAGACACT 
TGGAACCGGC TTGACTTTTT CATCGTCATT GCAGGGATGC TGGAGTATTC GCTGGACCTG 
CAGAATGTCA GCTTCTCAGC TGTCAGGACA GTCCGTGTGC TTCGACCCCT CAGGGCCATT 
AACCGGGTGC CCAGCATGCG CATCCTCGTC ACGCTGCTGC TGGACACGCT GCCCATGCTG 
GGCAACGTCC TGCTGCTCTG CTTCTTCGTC TTCTTCATCT TCGGCATCGT TGGTGTCCAG 
CTGTGGGCAG GACTGCTTCG TAACCGATGC TTCCTGCCTG AGAATTTCAG CCTCCCCCTG 
AGCGTGGACT TGGAGCGCTA CTACCAGACT GAGAACGAGG ATGAGAACCC ATTCATCTGC 
TCCCAGCCTC GTGAAAACGG CATGCGCTCC TGCAGGAGTG TGCCCACGCT GCGCGGGGAC 
GGGGGTGGCG GCCCACCCTG CGGCCTGGAC TACGAGGCCT ACAACAGCTC CAGCAACACC 
ACCTGTGTGA ACTGGAACCA GTACTACACC AACTGCTCCG CGGGGGAGCA CAACCCCTTC 
AAGGGTGCCA TCAACTTCGA CAACATTGGC TATGCCTGGA TCGCCATCTT CCAGGTCATC 
ACGCTGGAGG GCTGGGTCGA CATCATGTAC TTTGTGATGG ATGCTCACTC CTTCTACAAC 
TTCATCTACT TCATCCTTCT CATCATTGTG GGCTCTTTCT TTATGATCAA CCTGTGCCTG 
GTGGTGATTG CCACGCAGTT CTCAGAGACC AAGCAGCGGG AGAGCCAGCT GATGCGGGAG 
CAGCGCGTGA GATTCCTTTC CAACGCCAGC ACCCTGGCCA GCTTCTCCGA GCCCGGCAGC 
TGCTATGAGG AGCTGCTCAA GTACCTCGTG TATATCCTCC GGAAAGCAGC CCGAAGGCTG 
GCCCAGGTCT CCCGGGCGGC GGGCGTGCGG GTTGGCCTGC TGAGCAGCCC AGCGCCCCGT 
GGGGGCCAGG AGCCCCAGCC CAGTGGCGGC TGCCCTCGCC CCCACCGCCG TCCATCCGTC 
CACCATCTGG TGCACCACCA CCACCACCAC CACTACCACT TGGGCAATGG GACTCTGCGG 
GCCCCTCGGC CCAGCCCGCA GATCCAGGAC AGAGATGCCA GTGGGTCCCG CCGGCTCGTG 
CTGCCACCAC CCTCAACTCC TGCCCTTTCC AGGGGCCCTC CAGGGGGTGC AGAGGCTGTG 
CACAGCTTCT ACCATGCTGA TTGCCACTTG GAGCCAGTCC GCTGCCAGGC ACCCCCTCCC 
AGGTCGCCAT CGGAGGCATC GGGCAGGACC GTGGACAGTG GGAAGGTGTA CCCCACCGTG 
CACACCAGCC CTCCACCTGA GATGCTCAAA GAGAAGGCAC TAATGGAGGT GGCGCCCGCC 
TCTGGACCCA CCACCCTCAC CAGCCTCAAC ATCCCGCCCA GGCCCTACAG CTCTATGCAC 
AAGCTGCTGG AGACCCAGAG CACAGGCGCC TGCCAAAGCT CTTGCAAGAT CTCCAGCCCT 
TGCTTGAGGG CAGCCAGCGG AGCCTGTGGC CCAGACAGCT GTCCCTACTG TGCCCGGACC 
GGGGCAGGGG AGGTGGGGCT CACTGGCCAC GAGATGCCTG ACTCAGACAG TGAGGCGGTT 
TATGAGTTCA CACAAGATGC TCAGCATGGC GACCTCCGGG ATCCCCACAG TCGGCGTCGA 
CGGAGCCTGG GGCTGGATGT GGAGCCCAAC TCTGTGCTGG CCTTCTGGAG GCTGATCTGT 
GACACCTTCC GGAAGATTGT GGACAGCAAG TACTTTGGTC GGGGAATCAT GATTGCTATT 
CTGGTCAACA CGCTCAGCAT GGGCATCGAA TACCACGAGC AGCCTGAGGA GCTCACCAAT 
GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTTG CCCTGGAGAT GCTGCTGAAG 
CTGCTAGTAT ATGGTCCCTT TGGCTACATT AAGAACCCCT ACAACATCTT TGATGGCGTC 
ATTGTTGTCA TCAGCGTGTG GGAGATCGTG GGCCAGCAGG GGGGCGGCCT GTCGGTGCTG 
CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTGGTGCGCT TCTTGCCGGC GCTGCAGCGG 
CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGT ATGCATCTCT TCGGCTGCAA ATTTGCATCT 
GAGCGGGATG GGGACACGTT GCCGGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATC 
GTCACTGTCT TTCAGATCCT GACTCAGGAG GACTGGAACA AGGTGCTCTA CAACGGAATG 
GCCTCCACGT CATCCTGGGC TGCCCTTTAC TTCATCGCCC TCATGACCTT CGGCAACTAT 
GTGCTCTTCA ACCTGCTCGT TGCCATTCTG GTGGAGGGCT TCCAAACGGA GGAAATCAGC 
AAACGGGAAG ATGCGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCGA CTCCCAGGGG 
GGAGATGCCA CCAAGTCAGA GTCGGAACCT GATTTCTTCT CGCCCAGCCT GGATGGTGAT 
GGGGACAGGA AGAAGCGCTT GGCCTTGGTG TCTCTGGGAG AGCACCCGGA GCTGCGGAGG 
AGCTTGTTAC CACCGCTCAT CATCCACACG GCTGCCACTC CCATGTCATT GCCCAAAAGC 
TCCAGCAGCG GCCTGGCCGA GGTTCTGGGC CCCCCCTCCC GCCGCACCAG CAGCAGTGGG 
TCGGCTGAGC CAGGAGCACC TCACGAGATG AAGTCACCGC CAAGTGTCCG CAGTTCCCCC 
CACAGCCCCT GGAGTGCAGC AAGCAGCTGG ACGAGCAGGC GCTCCAGCAG GAACAGCCTG 
GGCCGTGCCC CCAGCCTGAA GCGAAGGAGC CCAAGTGGGG AGCGGAGGTC CTTGTTGTCG 
GGTGAGGGCC GGGAGAGCCA GGATGAGGAG GAGAGCTCGG AAGAGGAGCG CACTAGCCCA 
GCGGGCAGTG ACCGCCACCA CAGGGGCTCC CTGGAGAGGG AAGCTAAGAG CTCCTTTGAC 
CTACCGGACA CACTGCAGGT TCCCGGGCTG TACCGCACAG CCAGCGGCCG CAGCTCAGCC 
TCTGAGCACC AGGACTGCAA TGGCAATTCG GCCGTGGGAC GCCTGAGTCA GGCTGTGCGG 
CCCGATGACC CCCCGTTGGA TGGAGACGAT GGTGATGACG AGGGCAACTT GAGCAAAGCA 
GAACGGCTGA GGGCGTGGAT CCGAACCCGG CTCCCTGTCT GCTGCCTGGA GCGAGACTCT 
TGGTCTGCCT ACATCTTCCC TCCTCAGTCA AGGTTCCGCC TCCTGTGTCA CCGAATCATC 
ACCCACAAGA TGTTTGACCA CGTGGTCCTC GTCATCATCT TCCTGAACTG TATCACCATC 
GCCATGGAGC GCCCCAAGAT CGACCCCCAC AGCGCTGAAC GAATTTTCCT GACCCTCTCC 
AATTACATCT TCACCGCAGT CTTTCTGGCT GAGATGACAG TGAAGGTGGT GGCGCTGGGC 
TGGTGTTTTG GGGAGCAGGC CTACCTGCGT AGCAGCTGGA ACGTGCTGGA CGGGCTGCTG 
GTGCTCATCT CCATCATCGA CATCCTGGTG TCCATGGTCT CCGACAGCGG CACCAAGATC 
CTGGGCATGC TGAGGGTACT GCGGCTGCTG CGGACCCTGC GCCCGCTCAG GGTAATCAGC 
CGGGCACAGG GGCTGAAGCT GGTGGTGGAA ACGCTGATGT CCTCGCTGAA GCCCATTGGC 
AACATTGTGG TCATCTGTTG TGCCTTCTTC ATCATATTTG GCATCCTAGG GGTGCAGCTC 
TTCAAAGGGA AGTTCTTTGT GTGCCAGGGC GAGGACACCA GGAACATCAC GAACAAGTCT 
GACTGTGCTG AGGCCAGTTA CCGGTGGGTC CGGCACAAAT ACAACTTTGA CAACCTCGGC 
CAGGCCCTAA TGTCCCTGTT CGTGCTGGCC TCTAAGGATG GCTGGGTGGA TATCATGTAT 
GATGGGCTGG ATGCTGTCGG CGTGGACCAG CAGCCCATCA TGAACCACAA CCCCTGGATG 
CTGCTGTACT TCATCTCGTT CCTGCTCATC GTGGCCTTCT TTGTCCTGAA CATGTTCGTT 
GGTGTGGTGG TGGAGAACTT CCACAAGTGT CGGCAGCACC AGGAGGAAGA GGAGGCCCGG 
CGGCGGGAGG AGAAGCGTCT ACGGAGACTG GAGAAAAAGA GAAGGAATCT AATGCTGGAC 
GATGTAATTG CTTCCGGCAG CTCAGCCAGC GCGGCGCCAG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCC GCAGCCCAGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TGGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220507.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220564.1 Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.

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CloneID	OBa226004
Clone ID Related Accession (Same CDS sequence)	XM_005220507.4
Accession Version	XM_005220507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC 
GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG 
AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG 
AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG 
GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG 
CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG 
AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG 
GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG 
CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC 
TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC 
ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC 
AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG 
GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC 
TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC 
CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG 
GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220564.1
CDS	768..7508
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220507.4
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 G (CACNA1G), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220507.4

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCGC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAATGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC 
GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG 
AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG 
AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG 
GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG 
CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG 
AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG 
GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG 
CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC 
TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC 
ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC 
AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG 
GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC 
TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC 
CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG 
GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTGGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_015458770.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314256.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.

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CloneID	OBa93902
Clone ID Related Accession (Same CDS sequence)	XM_015458770.1
Accession Version	XM_015458770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT 
GCCTGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT 
GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC 
CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC 
CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG 
CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC 
TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC 
ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG 
CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC 
AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC 
AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC 
CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG 
GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC 
CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC 
AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC 
CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC 
AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314256.1
CDS	731..7804
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPACSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_015458770.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458770.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGGACA GACAGCTGTT TGACACCATA TCCCTGCTGA TCCAGGGCTC CCTGGATGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTCCT 
GCCTGCAGCC CAGGCGGCTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
TTGACGTCAG AGATTGTGTC TGAACTGTCC TGCTCTCTAG CTCTGACGGA TGACTGTCTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGCGA GCAATATGGA GTCCCACCCT 
GAGGAGGTGC CCGTCATGCT AGGACCAGAT CTGCTGACTG TGCGCAAGTC TGGGGTCAGC 
CGAACGCACT CTCTGCCCAA TGACAGCTAC ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC 
CACTCCCAGC CAGCAGACAC CAGCTACATC CCGCAGCTTC CCAAAGATGC GCCCCATCTG 
CTCCAGCCCC ATGGTGCTCC CACCTGGGGC ACCATCCCCA AATTGCCCCC ACCAGGCCGC 
TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC CAGGCAGCAA TACGGACGGA TTCCCTGGAC 
ATTCAGGGCC TGGGCAGCAG GGAAGACCTG CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG 
CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC TCCAGCACCC AGGCTCAGCA GCATTCCCGC 
AGCCAGAGCA AGATCTTGAA GCACATGCCC CCGTCAGCCC CCTGCCCAGG CCCAGAACTC 
AAGTGGGCCA AGGGCCCACC AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCACGGAC AGTCAGGAGG AAGCCCCGTC TGCACGGGAC 
CTGAAGAAGT GCTACAGCGT GGAGGCGCAG AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG 
GATGAACAGA GGAGGCACTC CATCGCGGTC AGCTGCCTGG ACAGTGGCTC CCAACCCGCC 
CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC 
AAGAAGAAAC TCAGCCCACC CAGCATCTCC ATAGACCCCC CAGAGAGCCA GAGCCAGGGC 
CCTCGGCCCC CACCCAGTCC TGGTGTCTGC CTCCGCCGGA GGGCTCCGTC CAGCGACTCC 
AAGGATCCCT TGGCCTCTGG CCCTCCTGAC AGCATGGCTG CCTCGCCCTC CCCAAAGAAA 
GACGTGCTGA GTCTCTCTGG TTTATCCTCT GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_015458771.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314257.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.

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CloneID	OBa93903
Clone ID Related Accession (Same CDS sequence)	XM_015458771.1
Accession Version	XM_015458771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT 
GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC 
ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG 
CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA 
GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT 
GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC 
AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC 
TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC 
TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC 
CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC 
AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG 
CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC 
TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC 
CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC 
AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC 
TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC 
TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314257.1
CDS	731..7729
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_015458771.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458771.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTTTGAC GTCAGAGATT 
GTGTCTGAAC TGTCCTGCTC TCTAGCTCTG ACGGATGACT GTCTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGCGAGCAAT ATGGAGTCCC ACCCTGAGGA GGTGCCCGTC 
ATGCTAGGAC CAGATCTGCT GACTGTGCGC AAGTCTGGGG TCAGCCGAAC GCACTCTCTG 
CCCAATGACA GCTACATGTG CCGGGATGGG AGCACTGCCG AGGGGTCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAGAGC TCAGTCAGGC TCCATCTTGT CCGTCCACTC CCAGCCAGCA 
GACACCAGCT ACATCCCGCA GCTTCCCAAA GATGCGCCCC ATCTGCTCCA GCCCCATGGT 
GCTCCCACCT GGGGCACCAT CCCCAAATTG CCCCCACCAG GCCGCTCCCC TTTGGCTCAG 
AGGCCACTCA GGCGCCAGGC AGCAATACGG ACGGATTCCC TGGACATTCA GGGCCTGGGC 
AGCAGGGAAG ACCTGCTGTC AGAGGTGAGT GGGCCCTCCC CGCCGCTGGC CCGGGCCTCC 
TCCTTTTGGG GCCACTCCAG CACCCAGGCT CAGCAGCATT CCCGCAGCCA GAGCAAGATC 
TTGAAGCACA TGCCCCCGTC AGCCCCCTGC CCAGGCCCAG AACTCAAGTG GGCCAAGGGC 
CCACCAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA CGGACAGTCA GGAGGAAGCC CCGTCTGCAC GGGACCTGAA GAAGTGCTAC 
AGCGTGGAGG CGCAGAGCTG CCCGCGCCGG CCGGCATCCT GGCTGGATGA ACAGAGGAGG 
CACTCCATCG CGGTCAGCTG CCTGGACAGT GGCTCCCAAC CCGCCCTGGG CCCTGATCCC 
TCGAGCCTTG GAGGTCAGCC TCTAGGGGGA GCTGGGAGCC GGCCCAAGAA GAAACTCAGC 
CCACCCAGCA TCTCCATAGA CCCCCCAGAG AGCCAGAGCC AGGGCCCTCG GCCCCCACCC 
AGTCCTGGTG TCTGCCTCCG CCGGAGGGCT CCGTCCAGCG ACTCCAAGGA TCCCTTGGCC 
TCTGGCCCTC CTGACAGCAT GGCTGCCTCG CCCTCCCCAA AGAAAGACGT GCTGAGTCTC 
TCTGGTTTAT CCTCTGACCC AGCAGACCTG GACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( NM_001193140.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180069.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), mRNA.

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CloneID	OBa40889
Clone ID Related Accession (Same CDS sequence)	NM_001193140.1
Accession Version	NM_001193140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03062363.1, AAFC03121466.1 and AAFC03062365.1. On Jul 17, 2010 this sequence version replaced XM_869265.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC 
CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC 
TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG 
GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT 
GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG 
TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT 
GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT 
GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC 
TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG 
CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA 
CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT 
TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC 
TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG 
CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC 
CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT 
GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA 
TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC 
CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG 
AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG 
AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC 
AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180069.1
CDS	1..7143
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPACSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	NM_001193140.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193140.1

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACCGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAATGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGGACAG ACAGCTGTTT GACACCATAT CCCTGCTGAT CCAGGGCTCC 
CTGGATGGGG AGCTGAAGCT GATGGACGAG CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC 
TTCCCTCCTG CCTGCAGCCC AGGCGGCTCC GACCCACAGA TCCCTCTAGC TGAGATGGAG 
GCTCTGTCTT TGACGTCAGA GATTGTGTCT GAACTGTCCT GCTCTCTAGC TCTGACGGAT 
GACTGTCTGC CTGATGACAC TCACACACTC TTACTTAGTG CCCTGGCGAG CAATATGGAG 
TCCCACCCTG AGGAGGTGCC CGTCATGCTA GGACCAGATC TGCTGACTGT GCGCAAGTCT 
GGGGTCAGCC GAACGCACTC TCTGCCCAAT GACAGCTACA TGTGCCGGGA TGGGAGCACT 
GCCGAGGGGT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA GAGCTCAGTC AGGCTCCATC 
TTGTCCGTCC ACTCCCAGCC AGCAGACACC AGCTACATCC CGCAGCTTCC CAAAGATGCG 
CCCCATCTGC TCCAGCCCCA TGGTGCTCCC ACCTGGGGCA CCATCCCCAA ATTGCCCCCA 
CCAGGCCGCT CCCCTTTGGC TCAGAGGCCA CTCAGGCGCC AGGCAGCAAT ACGGACGGAT 
TCCCTGGACA TTCAGGGCCT GGGCAGCAGG GAAGACCTGC TGTCAGAGGT GAGTGGGCCC 
TCCCCGCCGC TGGCCCGGGC CTCCTCCTTT TGGGGCCACT CCAGCACCCA GGCTCAGCAG 
CATTCCCGCA GCCAGAGCAA GATCTTGAAG CACATGCCCC CGTCAGCCCC CTGCCCAGGC 
CCAGAACTCA AGTGGGCCAA GGGCCCACCA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCACGGACA GTCAGGAGGA AGCCCCGTCT 
GCACGGGACC TGAAGAAGTG CTACAGCGTG GAGGCGCAGA GCTGCCCGCG CCGGCCGGCA 
TCCTGGCTGG ATGAACAGAG GAGGCACTCC ATCGCGGTCA GCTGCCTGGA CAGTGGCTCC 
CAACCCGCCC TGGGCCCTGA TCCCTCGAGC CTTGGAGGTC AGCCTCTAGG GGGAGCTGGG 
AGCCGGCCCA AGAAGAAACT CAGCCCACCC AGCATCTCCA TAGACCCCCC AGAGAGCCAG 
AGCCAGGGCC CTCGGCCCCC ACCCAGTCCT GGTGTCTGCC TCCGCCGGAG GGCTCCGTCC 
AGCGACTCCA AGGATCCCTT GGCCTCTGGC CCTCCTGACA GCATGGCTGC CTCGCCCTCC 
CCAAAGAAAG ACGTGCTGAG TCTCTCTGGT TTATCCTCTG ACCCAGCAGA CCTGGACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220487.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220544.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.

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CloneID	OBa29847
Clone ID Related Accession (Same CDS sequence)	XM_005220487.3
Accession Version	XM_005220487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCT GCAGCCCAGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220544.1
CDS	731..7840
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPACSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220487.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220487.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGGACAGACA GCTGTTTGAC 
ACCATATCCC TGCTGATCCA GGGCTCCCTG GATGGGGAGC TGAAGCTGAT GGACGAGCTG 
GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC CCTCCTGCCT GCAGCCCAGG CGGCTCCGAC 
CCACAGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220488.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220545.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.

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CloneID	OBa29848
Clone ID Related Accession (Same CDS sequence)	XM_005220488.3
Accession Version	XM_005220488.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC 
CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC TGCAGCCCAG GCGGCTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC 
TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220545.1
CDS	731..7771
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMDRQLFDTISLLIQGSLDGELKLMDELAGPGGQPSAFPPACSPGGSDPQIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220488.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220488.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGGACAGAC AGCTGTTTGA CACCATATCC 
CTGCTGATCC AGGGCTCCCT GGATGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTCCTGCC TGCAGCCCAG GCGGCTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTTTG ACGTCAGAGA TTGTGTCTGA ACTGTCCTGC 
TCTCTAGCTC TGACGGATGA CTGTCTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGCGAGCA ATATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220494.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220551.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.

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CloneID	OBa29854
Clone ID Related Accession (Same CDS sequence)	XM_005220494.3
Accession Version	XM_005220494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220551.1
CDS	731..7660
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMIPLAEMEALSLTSEIVSELSCSLALTDDCLPDDTHTLLLSALASNMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220494.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220494.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATCC CTCTAGCTGA GATGGAGGCT CTGTCTTTGA CGTCAGAGAT TGTGTCTGAA 
CTGTCCTGCT CTCTAGCTCT GACGGATGAC TGTCTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGCGAGCAA TATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220497.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220554.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.

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CloneID	OBa29857
Clone ID Related Accession (Same CDS sequence)	XM_005220497.3
Accession Version	XM_005220497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT 
CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC 
AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC 
CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC 
ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG 
CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC 
TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC 
CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC 
AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG 
AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC 
AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT 
CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC 
ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC 
CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC 
AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220554.1
CDS	730..7593
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220497.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220497.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA TCTAATGCTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCGGCGC CAGAAGCCCA GTGCAAACCC TACTACTCAG ACTACTCCCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGATCTCT TCATCACGGG TGTCATTGGG 
CTGAACGTGG TCACTATGGC CATGGAGCAC TACCAGCAGC CCCAGATCCT GGATGAGGCT 
CTGAAGATCT GCAACTATAT CTTCACTGTC ATCTTTGTCT TGGAGTCAGT TTTCAAACTG 
GTGGCCTTTG GCTTTCGACG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCCATT 
GTGCTGCTGT CCATCATGGG CATCACACTG GAGGAGATTG AGGTCAATGC CTCGCTGCCC 
ATCAACCCCA CCATCATCCG CATCATGAGG GTGCTGCGTA TTGCCCGAGT GCTAAAGCTA 
CTGAAGATGG CTGTGGGCAT GCGGGCACTC CTGGACACAG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACT TGGGACTTCT CTTCATGTTG TTGTTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGATCTGGA GTGTGATGAG ACGCACCCCT GCGAGGGCCT GGGCCGGCAT 
GCCACCTTTC GGAACTTTGG CATGGCCTTC TTGACCCTCT TCCGAGTCTC CACAGGTGAC 
AACTGGAATG GCATCATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACGGTCA TCTCCCCCAT CTACTTCGTT TCCTTCGTGC TGACAGCCCA GTTTGTGCTG 
GTCAACGTGG TCATTGCCGT GCTGATGAAG CATCTGGAGG AGAGCAACAA GGAGGCCAAG 
GAGGAGGCCG AGCTGGAGGC TGAACTGGAG CTAGAGATGA AGACGCTCAG CCCACAAGCC 
CCCTCACCAC TGGGCAGCCC CTTCCTCTGG CCTGGGGTCG AGGGCCCCGA CAGCCCTGGC 
AGCCCCAAAC CTGGGGCCCC ACATGCCACA CCCCACACTG GAGCAGCCTC CCGCTTCTCC 
CTGGAGCACC CCATGATGGA GTCCCACCCT GAGGAGGTGC CCGTCATGCT AGGACCAGAT 
CTGCTGACTG TGCGCAAGTC TGGGGTCAGC CGAACGCACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGGG ATGGGAGCAC TGCCGAGGGG TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC 
AGAGCTCAGT CAGGCTCCAT CTTGTCCGTC CACTCCCAGC CAGCAGACAC CAGCTACATC 
CCGCAGCTTC CCAAAGATGC GCCCCATCTG CTCCAGCCCC ATGGTGCTCC CACCTGGGGC 
ACCATCCCCA AATTGCCCCC ACCAGGCCGC TCCCCTTTGG CTCAGAGGCC ACTCAGGCGC 
CAGGCAGCAA TACGGACGGA TTCCCTGGAC ATTCAGGGCC TGGGCAGCAG GGAAGACCTG 
CTGTCAGAGG TGAGTGGGCC CTCCCCGCCG CTGGCCCGGG CCTCCTCCTT TTGGGGCCAC 
TCCAGCACCC AGGCTCAGCA GCATTCCCGC AGCCAGAGCA AGATCTTGAA GCACATGCCC 
CCGTCAGCCC CCTGCCCAGG CCCAGAACTC AAGTGGGCCA AGGGCCCACC AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCACGGAC 
AGTCAGGAGG AAGCCCCGTC TGCACGGGAC CTGAAGAAGT GCTACAGCGT GGAGGCGCAG 
AGCTGCCCGC GCCGGCCGGC ATCCTGGCTG GATGAACAGA GGAGGCACTC CATCGCGGTC 
AGCTGCCTGG ACAGTGGCTC CCAACCCGCC CTGGGCCCTG ATCCCTCGAG CCTTGGAGGT 
CAGCCTCTAG GGGGAGCTGG GAGCCGGCCC AAGAAGAAAC TCAGCCCACC CAGCATCTCC 
ATAGACCCCC CAGAGAGCCA GAGCCAGGGC CCTCGGCCCC CACCCAGTCC TGGTGTCTGC 
CTCCGCCGGA GGGCTCCGTC CAGCGACTCC AAGGATCCCT TGGCCTCTGG CCCTCCTGAC 
AGCATGGCTG CCTCGCCCTC CCCAAAGAAA GACGTGCTGA GTCTCTCTGG TTTATCCTCT 
GACCCAGCAG ACCTGGACCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220499.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220556.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.

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CloneID	OBa29859
Clone ID Related Accession (Same CDS sequence)	XM_005220499.3
Accession Version	XM_005220499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG 
GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA 
ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC 
CTAGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC 
TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC 
CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC 
CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT 
CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG 
GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC 
CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG 
TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG 
AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT 
GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG 
GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG 
AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT 
CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG 
GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220556.1
CDS	730..7560
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220499.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220499.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCTG AAGCCCAGTG CAAACCCTAC 
TACTCAGACT ACTCCCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GATCTCTTCA TCACGGGTGT CATTGGGCTG AACGTGGTCA CTATGGCCAT GGAGCACTAC 
CAGCAGCCCC AGATCCTGGA TGAGGCTCTG AAGATCTGCA ACTATATCTT CACTGTCATC 
TTTGTCTTGG AGTCAGTTTT CAAACTGGTG GCCTTTGGCT TTCGACGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCCATTGTG CTGCTGTCCA TCATGGGCAT CACACTGGAG 
GAGATTGAGG TCAATGCCTC GCTGCCCATC AACCCCACCA TCATCCGCAT CATGAGGGTG 
CTGCGTATTG CCCGAGTGCT AAAGCTACTG AAGATGGCTG TGGGCATGCG GGCACTCCTG 
GACACAGTGA TGCAGGCCCT GCCCCAGGTG GGGAACTTGG GACTTCTCTT CATGTTGTTG 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ATCTGGAGTG TGATGAGACG 
CACCCCTGCG AGGGCCTGGG CCGGCATGCC ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG 
ACCCTCTTCC GAGTCTCCAC AGGTGACAAC TGGAATGGCA TCATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACGGTCATCT CCCCCATCTA CTTCGTTTCC 
TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC AACGTGGTCA TTGCCGTGCT GATGAAGCAT 
CTGGAGGAGA GCAACAAGGA GGCCAAGGAG GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA 
GAGATGAAGA CGCTCAGCCC ACAAGCCCCC TCACCACTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTCGAGG GCCCCGACAG CCCTGGCAGC CCCAAACCTG GGGCCCCACA TGCCACACCC 
CACACTGGAG CAGCCTCCCG CTTCTCCCTG GAGCACCCCA TGATGGAGTC CCACCCTGAG 
GAGGTGCCCG TCATGCTAGG ACCAGATCTG CTGACTGTGC GCAAGTCTGG GGTCAGCCGA 
ACGCACTCTC TGCCCAATGA CAGCTACATG TGCCGGGATG GGAGCACTGC CGAGGGGTCC 
CTAGGACACA GGGGCTGGGG GCTCCCCAGA GCTCAGTCAG GCTCCATCTT GTCCGTCCAC 
TCCCAGCCAG CAGACACCAG CTACATCCCG CAGCTTCCCA AAGATGCGCC CCATCTGCTC 
CAGCCCCATG GTGCTCCCAC CTGGGGCACC ATCCCCAAAT TGCCCCCACC AGGCCGCTCC 
CCTTTGGCTC AGAGGCCACT CAGGCGCCAG GCAGCAATAC GGACGGATTC CCTGGACATT 
CAGGGCCTGG GCAGCAGGGA AGACCTGCTG TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG 
GCCCGGGCCT CCTCCTTTTG GGGCCACTCC AGCACCCAGG CTCAGCAGCA TTCCCGCAGC 
CAGAGCAAGA TCTTGAAGCA CATGCCCCCG TCAGCCCCCT GCCCAGGCCC AGAACTCAAG 
TGGGCCAAGG GCCCACCAGA GACCAGAAGC AGCTTAGAGC TGGACACGGA GCTGAGCTGG 
ATTTCAGGAG ACCTCCTGCC CACGGACAGT CAGGAGGAAG CCCCGTCTGC ACGGGACCTG 
AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC TGCCCGCGCC GGCCGGCATC CTGGCTGGAT 
GAACAGAGGA GGCACTCCAT CGCGGTCAGC TGCCTGGACA GTGGCTCCCA ACCCGCCCTG 
GGCCCTGATC CCTCGAGCCT TGGAGGTCAG CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG 
AAGAAACTCA GCCCACCCAG CATCTCCATA GACCCCCCAG AGAGCCAGAG CCAGGGCCCT 
CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC CGCCGGAGGG CTCCGTCCAG CGACTCCAAG 
GATCCCTTGG CCTCTGGCCC TCCTGACAGC ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC 
GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220501.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220558.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.

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CloneID	OBa29861
Clone ID Related Accession (Same CDS sequence)	XM_005220501.3
Accession Version	XM_005220501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220558.1
CDS	730..7539
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEISKREDASGQLSCIQLPVDSQGGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220501.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220501.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGAAATC AGCAAACGGG AAGATGCGAG TGGACAGTTA 
AGCTGTATTC AGCTGCCTGT CGACTCCCAG GGGGGAGATG CCACCAAGTC AGAGTCGGAA 
CCTGATTTCT TCTCGCCCAG CCTGGATGGT GATGGGGACA GGAAGAAGCG CTTGGCCTTG 
GTGTCTCTGG GAGAGCACCC GGAGCTGCGG AGGAGCTTGT TACCACCGCT CATCATCCAC 
ACGGCTGCCA CTCCCATGTC ATTGCCCAAA AGCTCCAGCA GCGGCCTGGC CGAGGTTCTG 
GGCCCCCCCT CCCGCCGCAC CAGCAGCAGT GGGTCGGCTG AGCCAGGAGC ACCTCACGAG 
ATGAAGTCAC CGCCAAGTGT CCGCAGTTCC CCCCACAGCC CCTGGAGTGC AGCAAGCAGC 
TGGACGAGCA GGCGCTCCAG CAGGAACAGC CTGGGCCGTG CCCCCAGCCT GAAGCGAAGG 
AGCCCAAGTG GGGAGCGGAG GTCCTTGTTG TCGGGTGAGG GCCGGGAGAG CCAGGATGAG 
GAGGAGAGCT CGGAAGAGGA GCGCACTAGC CCAGCGGGCA GTGACCGCCA CCACAGGGGC 
TCCCTGGAGA GGGAAGCTAA GAGCTCCTTT GACCTACCGG ACACACTGCA GGTTCCCGGG 
CTGTACCGCA CAGCCAGCGG CCGCAGCTCA GCCTCTGAGC ACCAGGACTG CAACGGCAAT 
TCGGCCGTGG GACGCCTGAG TCAGGCTGTG CGGCCCGATG ACCCCCCGTT GGATGGAGAC 
GATGGTGATG ACGAGGGCAA CTTGAGCAAA GCAGAACGGC TGAGGGCGTG GATCCGAACC 
CGGCTCCCTG TCTGCTGCCT GGAGCGAGAC TCTTGGTCTG CCTACATCTT CCCTCCTCAG 
TCAAGGTTCC GCCTCCTGTG TCACCGAATC ATCACCCACA AGATGTTTGA CCACGTGGTC 
CTCGTCATCA TCTTCCTGAA CTGTATCACC ATCGCCATGG AGCGCCCCAA GATCGACCCC 
CACAGCGCTG AACGAATTTT CCTGACCCTC TCCAATTACA TCTTCACCGC AGTCTTTCTG 
GCTGAGATGA CAGTGAAGGT GGTGGCGCTG GGCTGGTGTT TTGGGGAGCA GGCCTACCTG 
CGTAGCAGCT GGAACGTGCT GGACGGGCTG CTGGTGCTCA TCTCCATCAT CGACATCCTG 
GTGTCCATGG TCTCCGACAG CGGCACCAAG ATCCTGGGCA TGCTGAGGGT ACTGCGGCTG 
CTGCGGACCC TGCGCCCGCT CAGGGTAATC AGCCGGGCAC AGGGGCTGAA GCTGGTGGTG 
GAAACGCTGA TGTCCTCGCT GAAGCCCATT GGCAACATTG TGGTCATCTG TTGTGCCTTC 
TTCATCATAT TTGGCATCCT AGGGGTGCAG CTCTTCAAAG GGAAGTTCTT TGTGTGCCAG 
GGCGAGGACA CCAGGAACAT CACGAACAAG TCTGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AATACAACTT TGACAACCTC GGCCAGGCCC TAATGTCCCT GTTCGTGCTG 
GCCTCTAAGG ATGGCTGGGT GGATATCATG TATGATGGGC TGGATGCTGT CGGCGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCCTGG ATGCTGCTGT ACTTCATCTC GTTCCTGCTC 
ATCGTGGCCT TCTTTGTCCT GAACATGTTC GTTGGTGTGG TGGTGGAGAA CTTCCACAAG 
TGTCGGCAGC ACCAGGAGGA AGAGGAGGCC CGGCGGCGGG AGGAGAAGCG TCTACGGAGA 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCAGACTA CTCCCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ATCTCTTCAT CACGGGTGTC 
ATTGGGCTGA ACGTGGTCAC TATGGCCATG GAGCACTACC AGCAGCCCCA GATCCTGGAT 
GAGGCTCTGA AGATCTGCAA CTATATCTTC ACTGTCATCT TTGTCTTGGA GTCAGTTTTC 
AAACTGGTGG CCTTTGGCTT TCGACGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCCATTGTGC TGCTGTCCAT CATGGGCATC ACACTGGAGG AGATTGAGGT CAATGCCTCG 
CTGCCCATCA ACCCCACCAT CATCCGCATC ATGAGGGTGC TGCGTATTGC CCGAGTGCTA 
AAGCTACTGA AGATGGCTGT GGGCATGCGG GCACTCCTGG ACACAGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACTTGGG ACTTCTCTTC ATGTTGTTGT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA TCTGGAGTGT GATGAGACGC ACCCCTGCGA GGGCCTGGGC 
CGGCATGCCA CCTTTCGGAA CTTTGGCATG GCCTTCTTGA CCCTCTTCCG AGTCTCCACA 
GGTGACAACT GGAATGGCAT CATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CGGTCATCTC CCCCATCTAC TTCGTTTCCT TCGTGCTGAC AGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT TGCCGTGCTG ATGAAGCATC TGGAGGAGAG CAACAAGGAG 
GCCAAGGAGG AGGCCGAGCT GGAGGCTGAA CTGGAGCTAG AGATGAAGAC GCTCAGCCCA 
CAAGCCCCCT CACCACTGGG CAGCCCCTTC CTCTGGCCTG GGGTCGAGGG CCCCGACAGC 
CCTGGCAGCC CCAAACCTGG GGCCCCACAT GCCACACCCC ACACTGGAGC AGCCTCCCGC 
TTCTCCCTGG AGCACCCCAT GATGGAGTCC CACCCTGAGG AGGTGCCCGT CATGCTAGGA 
CCAGATCTGC TGACTGTGCG CAAGTCTGGG GTCAGCCGAA CGCACTCTCT GCCCAATGAC 
AGCTACATGT GCCGGGATGG GAGCACTGCC GAGGGGTCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAGAG CTCAGTCAGG CTCCATCTTG TCCGTCCACT CCCAGCCAGC AGACACCAGC 
TACATCCCGC AGCTTCCCAA AGATGCGCCC CATCTGCTCC AGCCCCATGG TGCTCCCACC 
TGGGGCACCA TCCCCAAATT GCCCCCACCA GGCCGCTCCC CTTTGGCTCA GAGGCCACTC 
AGGCGCCAGG CAGCAATACG GACGGATTCC CTGGACATTC AGGGCCTGGG CAGCAGGGAA 
GACCTGCTGT CAGAGGTGAG TGGGCCCTCC CCGCCGCTGG CCCGGGCCTC CTCCTTTTGG 
GGCCACTCCA GCACCCAGGC TCAGCAGCAT TCCCGCAGCC AGAGCAAGAT CTTGAAGCAC 
ATGCCCCCGT CAGCCCCCTG CCCAGGCCCA GAACTCAAGT GGGCCAAGGG CCCACCAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
ACGGACAGTC AGGAGGAAGC CCCGTCTGCA CGGGACCTGA AGAAGTGCTA CAGCGTGGAG 
GCGCAGAGCT GCCCGCGCCG GCCGGCATCC TGGCTGGATG AACAGAGGAG GCACTCCATC 
GCGGTCAGCT GCCTGGACAG TGGCTCCCAA CCCGCCCTGG GCCCTGATCC CTCGAGCCTT 
GGAGGTCAGC CTCTAGGGGG AGCTGGGAGC CGGCCCAAGA AGAAACTCAG CCCACCCAGC 
ATCTCCATAG ACCCCCCAGA GAGCCAGAGC CAGGGCCCTC GGCCCCCACC CAGTCCTGGT 
GTCTGCCTCC GCCGGAGGGC TCCGTCCAGC GACTCCAAGG ATCCCTTGGC CTCTGGCCCT 
CCTGACAGCA TGGCTGCCTC GCCCTCCCCA AAGAAAGACG TGCTGAGTCT CTCTGGTTTA 
TCCTCTGACC CAGCAGACCT GGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220502.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220559.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.

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CloneID	OBa29862
Clone ID Related Accession (Same CDS sequence)	XM_005220502.3
Accession Version	XM_005220502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT 
GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG 
GATGGGAGCA CTGCCGAGGG GTCCCTAGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG 
TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT 
CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC 
AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG 
GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC 
CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC 
CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG 
GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG 
CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG 
GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA 
GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC 
CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG 
AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT 
GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220559.1
CDS	730..7524
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAAPEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220502.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220502.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA ATCTAATGCT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCGGCG 
CCAGAAGCCC AGTGCAAACC CTACTACTCA GACTACTCCC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGATCTC TTCATCACGG GTGTCATTGG GCTGAACGTG 
GTCACTATGG CCATGGAGCA CTACCAGCAG CCCCAGATCC TGGATGAGGC TCTGAAGATC 
TGCAACTATA TCTTCACTGT CATCTTTGTC TTGGAGTCAG TTTTCAAACT GGTGGCCTTT 
GGCTTTCGAC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCCAT TGTGCTGCTG 
TCCATCATGG GCATCACACT GGAGGAGATT GAGGTCAATG CCTCGCTGCC CATCAACCCC 
ACCATCATCC GCATCATGAG GGTGCTGCGT ATTGCCCGAG TGCTAAAGCT ACTGAAGATG 
GCTGTGGGCA TGCGGGCACT CCTGGACACA GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
TTGGGACTTC TCTTCATGTT GTTGTTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGATCTGG AGTGTGATGA GACGCACCCC TGCGAGGGCC TGGGCCGGCA TGCCACCTTT 
CGGAACTTTG GCATGGCCTT CTTGACCCTC TTCCGAGTCT CCACAGGTGA CAACTGGAAT 
GGCATCATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACGGTC 
ATCTCCCCCA TCTACTTCGT TTCCTTCGTG CTGACAGCCC AGTTTGTGCT GGTCAACGTG 
GTCATTGCCG TGCTGATGAA GCATCTGGAG GAGAGCAACA AGGAGGCCAA GGAGGAGGCC 
GAGCTGGAGG CTGAACTGGA GCTAGAGATG AAGACGCTCA GCCCACAAGC CCCCTCACCA 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTC GAGGGCCCCG ACAGCCCTGG CAGCCCCAAA 
CCTGGGGCCC CACATGCCAC ACCCCACACT GGAGCAGCCT CCCGCTTCTC CCTGGAGCAC 
CCCATGATGG AGTCCCACCC TGAGGAGGTG CCCGTCATGC TAGGACCAGA TCTGCTGACT 
GTGCGCAAGT CTGGGGTCAG CCGAACGCAC TCTCTGCCCA ATGACAGCTA CATGTGCCGG 
GATGGGAGCA CTGCCGAGGG GTCCCTAGGA CACAGGGGCT GGGGGCTCCC CAGAGCTCAG 
TCAGGCTCCA TCTTGTCCGT CCACTCCCAG CCAGCAGACA CCAGCTACAT CCCGCAGCTT 
CCCAAAGATG CGCCCCATCT GCTCCAGCCC CATGGTGCTC CCACCTGGGG CACCATCCCC 
AAATTGCCCC CACCAGGCCG CTCCCCTTTG GCTCAGAGGC CACTCAGGCG CCAGGCAGCA 
ATACGGACGG ATTCCCTGGA CATTCAGGGC CTGGGCAGCA GGGAAGACCT GCTGTCAGAG 
GTGAGTGGGC CCTCCCCGCC GCTGGCCCGG GCCTCCTCCT TTTGGGGCCA CTCCAGCACC 
CAGGCTCAGC AGCATTCCCG CAGCCAGAGC AAGATCTTGA AGCACATGCC CCCGTCAGCC 
CCCTGCCCAG GCCCAGAACT CAAGTGGGCC AAGGGCCCAC CAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCACGGA CAGTCAGGAG 
GAAGCCCCGT CTGCACGGGA CCTGAAGAAG TGCTACAGCG TGGAGGCGCA GAGCTGCCCG 
CGCCGGCCGG CATCCTGGCT GGATGAACAG AGGAGGCACT CCATCGCGGT CAGCTGCCTG 
GACAGTGGCT CCCAACCCGC CCTGGGCCCT GATCCCTCGA GCCTTGGAGG TCAGCCTCTA 
GGGGGAGCTG GGAGCCGGCC CAAGAAGAAA CTCAGCCCAC CCAGCATCTC CATAGACCCC 
CCAGAGAGCC AGAGCCAGGG CCCTCGGCCC CCACCCAGTC CTGGTGTCTG CCTCCGCCGG 
AGGGCTCCGT CCAGCGACTC CAAGGATCCC TTGGCCTCTG GCCCTCCTGA CAGCATGGCT 
GCCTCGCCCT CCCCAAAGAA AGACGTGCTG AGTCTCTCTG GTTTATCCTC TGACCCAGCA 
GACCTGGACC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220505.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220562.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.

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CloneID	OBa29865
Clone ID Related Accession (Same CDS sequence)	XM_005220505.3
Accession Version	XM_005220505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC 
GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA 
GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT 
GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG 
GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC 
TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG 
ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG 
GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC 
TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG 
AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT 
CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC 
AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA 
CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG 
GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220562.1
CDS	730..7491
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220505.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220505.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA GTAAGGAGAA GCAGATGGCT GAAGCCCAGT GCAAACCCTA CTACTCAGAC 
TACTCCCGCT TCCGGCTCCT CGTCCACCAC CTGTGTACCA GCCACTACCT GGATCTCTTC 
ATCACGGGTG TCATTGGGCT GAACGTGGTC ACTATGGCCA TGGAGCACTA CCAGCAGCCC 
CAGATCCTGG ATGAGGCTCT GAAGATCTGC AACTATATCT TCACTGTCAT CTTTGTCTTG 
GAGTCAGTTT TCAAACTGGT GGCCTTTGGC TTTCGACGGT TCTTCCAGGA CAGGTGGAAC 
CAGCTGGACC TGGCCATTGT GCTGCTGTCC ATCATGGGCA TCACACTGGA GGAGATTGAG 
GTCAATGCCT CGCTGCCCAT CAACCCCACC ATCATCCGCA TCATGAGGGT GCTGCGTATT 
GCCCGAGTGC TAAAGCTACT GAAGATGGCT GTGGGCATGC GGGCACTCCT GGACACAGTG 
ATGCAGGCCC TGCCCCAGGT GGGGAACTTG GGACTTCTCT TCATGTTGTT GTTTTTCATC 
TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA GATCTGGAGT GTGATGAGAC GCACCCCTGC 
GAGGGCCTGG GCCGGCATGC CACCTTTCGG AACTTTGGCA TGGCCTTCTT GACCCTCTTC 
CGAGTCTCCA CAGGTGACAA CTGGAATGGC ATCATGAAGG ACACCCTCCG GGACTGTGAC 
CAGGAGTCCA CCTGCTACAA CACGGTCATC TCCCCCATCT ACTTCGTTTC CTTCGTGCTG 
ACAGCCCAGT TTGTGCTGGT CAACGTGGTC ATTGCCGTGC TGATGAAGCA TCTGGAGGAG 
AGCAACAAGG AGGCCAAGGA GGAGGCCGAG CTGGAGGCTG AACTGGAGCT AGAGATGAAG 
ACGCTCAGCC CACAAGCCCC CTCACCACTG GGCAGCCCCT TCCTCTGGCC TGGGGTCGAG 
GGCCCCGACA GCCCTGGCAG CCCCAAACCT GGGGCCCCAC ATGCCACACC CCACACTGGA 
GCAGCCTCCC GCTTCTCCCT GGAGCACCCC ATGATGGAGT CCCACCCTGA GGAGGTGCCC 
GTCATGCTAG GACCAGATCT GCTGACTGTG CGCAAGTCTG GGGTCAGCCG AACGCACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGGGAT GGGAGCACTG CCGAGGGGTC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAG AGCTCAGTCA GGCTCCATCT TGTCCGTCCA CTCCCAGCCA 
GCAGACACCA GCTACATCCC GCAGCTTCCC AAAGATGCGC CCCATCTGCT CCAGCCCCAT 
GGTGCTCCCA CCTGGGGCAC CATCCCCAAA TTGCCCCCAC CAGGCCGCTC CCCTTTGGCT 
CAGAGGCCAC TCAGGCGCCA GGCAGCAATA CGGACGGATT CCCTGGACAT TCAGGGCCTG 
GGCAGCAGGG AAGACCTGCT GTCAGAGGTG AGTGGGCCCT CCCCGCCGCT GGCCCGGGCC 
TCCTCCTTTT GGGGCCACTC CAGCACCCAG GCTCAGCAGC ATTCCCGCAG CCAGAGCAAG 
ATCTTGAAGC ACATGCCCCC GTCAGCCCCC TGCCCAGGCC CAGAACTCAA GTGGGCCAAG 
GGCCCACCAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCACGGACAG TCAGGAGGAA GCCCCGTCTG CACGGGACCT GAAGAAGTGC 
TACAGCGTGG AGGCGCAGAG CTGCCCGCGC CGGCCGGCAT CCTGGCTGGA TGAACAGAGG 
AGGCACTCCA TCGCGGTCAG CTGCCTGGAC AGTGGCTCCC AACCCGCCCT GGGCCCTGAT 
CCCTCGAGCC TTGGAGGTCA GCCTCTAGGG GGAGCTGGGA GCCGGCCCAA GAAGAAACTC 
AGCCCACCCA GCATCTCCAT AGACCCCCCA GAGAGCCAGA GCCAGGGCCC TCGGCCCCCA 
CCCAGTCCTG GTGTCTGCCT CCGCCGGAGG GCTCCGTCCA GCGACTCCAA GGATCCCTTG 
GCCTCTGGCC CTCCTGACAG CATGGCTGCC TCGCCCTCCC CAAAGAAAGA CGTGCTGAGT 
CTCTCTGGTT TATCCTCTGA CCCAGCAGAC CTGGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1G ( XM_005220507.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220564.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.

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CloneID	OBa29867
Clone ID Related Accession (Same CDS sequence)	XM_005220507.3
Accession Version	XM_005220507.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC 
GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG 
AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG 
AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG 
GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG 
CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG 
AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG 
GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG 
CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC 
TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC 
ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC 
AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG 
GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC 
TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC 
CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG 
GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220564.1
CDS	730..7470
Translation	MDEEEDGAGAEESGQPRSFMRLNDLSGARDRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDENPFICSQPRENGMRSCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPRGGQEPQPSGGCPRPHRRPSVHHLVHHHHHHHYHLGNGTLRAPRPSPQIQDRDASGSRRLVLPPPSTPALSRGPPGGAEAVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVDSGKVYPTVHTSPPPEMLKEKALMEVAPASGPTTLTSLNIPPRPYSSMHKLLETQSTGACQSSCKISSPCLRAASGACGPDSCPYCARTGAGEVGLTGHEMPDSDSEAVYEFTQDAQHGDLRDPHSRRRRSLGLDVEPNSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDATKSESEPDFFSPSLDGDGDRKKRLALVSLGEHPELRRSLLPPLIIHTAATPMSLPKSSSSGLAEVLGPPSRRTSSSGSAEPGAPHEMKSPPSVRSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGRESQDEEESSEEERTSPAGSDRHHRGSLEREAKSSFDLPDTLQVPGLYRTASGRSSASEHQDCNGNSAVGRLSQAVRPDDPPLDGDDGDDEGNLSKAERLRAWIRTRLPVCCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISIIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQAPSPLGSPFLWPGVEGPDSPGSPKPGAPHATPHTGAASRFSLEHPMMESHPEEVPVMLGPDLLTVRKSGVSRTHSLPNDSYMCRDGSTAEGSLGHRGWGLPRAQSGSILSVHSQPADTSYIPQLPKDAPHLLQPHGAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDIQGLGSREDLLSEVSGPSPPLARASSFWGHSSTQAQQHSRSQSKILKHMPPSAPCPGPELKWAKGPPETRSSLELDTELSWISGDLLPTDSQEEAPSARDLKKCYSVEAQSCPRRPASWLDEQRRHSIAVSCLDSGSQPALGPDPSSLGGQPLGGAGSRPKKKLSPPSISIDPPESQSQGPRPPPSPGVCLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP

Target ORF information:

RefSeq Version	XM_005220507.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220507.3

ORF Insert Sequence:

ATGGACGAGG AGGAGGATGG AGCGGGCGCC GAGGAGTCAG GACAGCCCCG GAGCTTCATG 
CGGCTCAACG ACCTGTCGGG GGCCAGGGAC CGGCCGGGGC CGGGGTCGGC GGAAAAGGAC 
CCCGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCATACC CTGCACTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCCTGGTTTG AGCGCATCAG TATGTTGGTC ATCCTTCTCA ACTGTGTGAC CCTGGGCATG 
TTCCGGCCAT GTGAAGACAT CGCCTGTGAC TCCCAGCGCT GCCGGATTCT GCAGGCCTTT 
GATGACTTCA TCTTTGCCTT CTTCGCTGTG GAGATGGTGG TGAAGATGGT GGCCTTGGGC 
ATCTTTGGAA AAAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTCATCGTC 
ATTGCAGGGA TGCTGGAGTA TTCGCTGGAC CTGCAGAATG TCAGCTTCTC AGCTGTCAGG 
ACAGTCCGTG TGCTTCGACC CCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATCCTC 
GTCACGCTGC TGCTGGACAC GCTGCCCATG CTGGGCAACG TCCTGCTGCT CTGCTTCTTC 
GTCTTCTTCA TCTTCGGCAT CGTTGGTGTC CAGCTGTGGG CAGGACTGCT TCGTAACCGA 
TGCTTCCTGC CTGAGAATTT CAGCCTCCCC CTGAGCGTGG ACTTGGAGCG CTACTACCAG 
ACTGAGAACG AGGATGAGAA CCCATTCATC TGCTCCCAGC CTCGTGAAAA CGGCATGCGC 
TCCTGCAGGA GTGTGCCCAC GCTGCGCGGG GACGGGGGTG GCGGCCCACC CTGCGGCCTG 
GACTACGAGG CCTACAACAG CTCCAGCAAC ACCACCTGTG TGAACTGGAA CCAGTACTAC 
ACCAACTGCT CCGCGGGGGA GCACAACCCC TTCAAGGGTG CCATCAACTT CGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACGCTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA CTCCTTCTAC AACTTCATCT ACTTCATCCT TCTCATCATT 
GTGGGCTCTT TCTTTATGAT CAACCTGTGC CTGGTGGTGA TTGCCACGCA GTTCTCAGAG 
ACCAAGCAGC GGGAGAGCCA GCTGATGCGG GAGCAGCGCG TGAGATTCCT TTCCAACGCC 
AGCACCCTGG CCAGCTTCTC CGAGCCCGGC AGCTGCTATG AGGAGCTGCT CAAGTACCTC 
GTGTATATCC TCCGGAAAGC AGCCCGAAGG CTGGCCCAGG TCTCCCGGGC GGCGGGCGTG 
CGGGTTGGCC TGCTGAGCAG CCCAGCGCCC CGTGGGGGCC AGGAGCCCCA GCCCAGTGGC 
GGCTGCCCTC GCCCCCACCG CCGTCCATCC GTCCACCATC TGGTGCACCA CCACCACCAC 
CACCACTACC ACTTGGGCAA TGGGACTCTG CGGGCCCCTC GGCCCAGCCC GCAGATCCAG 
GACAGAGATG CCAGTGGGTC CCGCCGGCTC GTGCTGCCAC CACCCTCAAC TCCTGCCCTT 
TCCAGGGGCC CTCCAGGGGG TGCAGAGGCT GTGCACAGCT TCTACCATGC TGATTGCCAC 
TTGGAGCCAG TCCGCTGCCA GGCACCCCCT CCCAGGTCGC CATCGGAGGC ATCGGGCAGG 
ACCGTGGACA GTGGGAAGGT GTACCCCACC GTGCACACCA GCCCTCCACC TGAGATGCTC 
AAAGAGAAGG CACTAATGGA GGTGGCGCCC GCCTCTGGAC CCACCACCCT CACCAGCCTC 
AACATCCCAC CCAGGCCCTA CAGCTCTATG CACAAGCTGC TGGAGACCCA GAGCACAGGC 
GCCTGCCAAA GCTCTTGCAA GATCTCCAGC CCTTGCTTGA GGGCAGCCAG CGGAGCCTGT 
GGCCCAGACA GCTGTCCCTA CTGTGCCCGG ACTGGGGCAG GGGAGGTGGG GCTCACTGGC 
CACGAGATGC CTGACTCAGA CAGTGAGGCG GTTTATGAGT TCACACAAGA TGCTCAGCAT 
GGCGACCTCC GGGATCCCCA CAGTCGGCGT CGACGGAGCC TGGGGCTGGA TGTGGAGCCC 
AACTCTGTGC TGGCCTTCTG GAGGCTGATC TGTGACACCT TCCGGAAGAT TGTGGACAGC 
AAGTACTTTG GTCGGGGAAT CATGATTGCT ATTCTGGTCA ACACGCTCAG CATGGGCATC 
GAATACCACG AGCAGCCTGA GGAGCTCACC AATGCCCTGG AAATCAGCAA CATCGTCTTC 
ACCAGCCTCT TTGCCCTGGA GATGCTGCTG AAGCTGCTAG TATATGGTCC CTTTGGCTAC 
ATTAAGAACC CCTACAACAT CTTTGATGGC GTCATTGTTG TCATCAGCGT GTGGGAGATC 
GTGGGCCAGC AGGGGGGCGG CCTGTCGGTG CTGCGGACCT TCCGCCTGAT GCGGGTGCTG 
AAGCTGGTGC GCTTCTTGCC GGCGCTGCAG CGGCAGCTCG TGGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGTATGCATC TCTTCGGCTG CAAATTTGCA TCTGAGCGGG ATGGGGACAC GTTGCCGGAC 
CGGAAGAATT TTGACTCCCT GCTCTGGGCC ATCGTCACTG TCTTTCAGAT CCTGACTCAG 
GAGGACTGGA ACAAGGTGCT CTACAACGGA ATGGCCTCCA CGTCATCCTG GGCTGCCCTT 
TACTTCATCG CCCTCATGAC CTTCGGCAAC TATGTGCTCT TCAACCTGCT CGTTGCCATT 
CTGGTGGAGG GCTTCCAAAC GGAGGGAGAT GCCACCAAGT CAGAGTCGGA ACCTGATTTC 
TTCTCGCCCA GCCTGGATGG TGATGGGGAC AGGAAGAAGC GCTTGGCCTT GGTGTCTCTG 
GGAGAGCACC CGGAGCTGCG GAGGAGCTTG TTACCACCGC TCATCATCCA CACGGCTGCC 
ACTCCCATGT CATTGCCCAA AAGCTCCAGC AGCGGCCTGG CCGAGGTTCT GGGCCCCCCC 
TCCCGCCGCA CCAGCAGCAG TGGGTCGGCT GAGCCAGGAG CACCTCACGA GATGAAGTCA 
CCGCCAAGTG TCCGCAGTTC CCCCCACAGC CCCTGGAGTG CAGCAAGCAG CTGGACGAGC 
AGGCGCTCCA GCAGGAACAG CCTGGGCCGT GCCCCCAGCC TGAAGCGAAG GAGCCCAAGT 
GGGGAGCGGA GGTCCTTGTT GTCGGGTGAG GGCCGGGAGA GCCAGGATGA GGAGGAGAGC 
TCGGAAGAGG AGCGCACTAG CCCAGCGGGC AGTGACCGCC ACCACAGGGG CTCCCTGGAG 
AGGGAAGCTA AGAGCTCCTT TGACCTACCG GACACACTGC AGGTTCCCGG GCTGTACCGC 
ACAGCCAGCG GCCGCAGCTC AGCCTCTGAG CACCAGGACT GCAACGGCAA TTCGGCCGTG 
GGACGCCTGA GTCAGGCTGT GCGGCCCGAT GACCCCCCGT TGGATGGAGA CGATGGTGAT 
GACGAGGGCA ACTTGAGCAA AGCAGAACGG CTGAGGGCGT GGATCCGAAC CCGGCTCCCT 
GTCTGCTGCC TGGAGCGAGA CTCTTGGTCT GCCTACATCT TCCCTCCTCA GTCAAGGTTC 
CGCCTCCTGT GTCACCGAAT CATCACCCAC AAGATGTTTG ACCACGTGGT CCTCGTCATC 
ATCTTCCTGA ACTGTATCAC CATCGCCATG GAGCGCCCCA AGATCGACCC CCACAGCGCT 
GAACGAATTT TCCTGACCCT CTCCAATTAC ATCTTCACCG CAGTCTTTCT GGCTGAGATG 
ACAGTGAAGG TGGTGGCGCT GGGCTGGTGT TTTGGGGAGC AGGCCTACCT GCGTAGCAGC 
TGGAACGTGC TGGACGGGCT GCTGGTGCTC ATCTCCATCA TCGACATCCT GGTGTCCATG 
GTCTCCGACA GCGGCACCAA GATCCTGGGC ATGCTGAGGG TACTGCGGCT GCTGCGGACC 
CTGCGCCCGC TCAGGGTAAT CAGCCGGGCA CAGGGGCTGA AGCTGGTGGT GGAAACGCTG 
ATGTCCTCGC TGAAGCCCAT TGGCAACATT GTGGTCATCT GTTGTGCCTT CTTCATCATA 
TTTGGCATCC TAGGGGTGCA GCTCTTCAAA GGGAAGTTCT TTGTGTGCCA GGGCGAGGAC 
ACCAGGAACA TCACGAACAA GTCTGACTGT GCTGAGGCCA GTTACCGGTG GGTCCGGCAC 
AAATACAACT TTGACAACCT CGGCCAGGCC CTAATGTCCC TGTTCGTGCT GGCCTCTAAG 
GATGGCTGGG TGGATATCAT GTATGATGGG CTGGATGCTG TCGGCGTGGA CCAGCAGCCC 
ATCATGAACC ACAACCCCTG GATGCTGCTG TACTTCATCT CGTTCCTGCT CATCGTGGCC 
TTCTTTGTCC TGAACATGTT CGTTGGTGTG GTGGTGGAGA ACTTCCACAA GTGTCGGCAG 
CACCAGGAGG AAGAGGAGGC CCGGCGGCGG GAGGAGAAGC GTCTACGGAG ACTGGAGAAA 
AAGAGAAGGA AAGCCCAGTG CAAACCCTAC TACTCAGACT ACTCCCGCTT CCGGCTCCTC 
GTCCACCACC TGTGTACCAG CCACTACCTG GATCTCTTCA TCACGGGTGT CATTGGGCTG 
AACGTGGTCA CTATGGCCAT GGAGCACTAC CAGCAGCCCC AGATCCTGGA TGAGGCTCTG 
AAGATCTGCA ACTATATCTT CACTGTCATC TTTGTCTTGG AGTCAGTTTT CAAACTGGTG 
GCCTTTGGCT TTCGACGGTT CTTCCAGGAC AGGTGGAACC AGCTGGACCT GGCCATTGTG 
CTGCTGTCCA TCATGGGCAT CACACTGGAG GAGATTGAGG TCAATGCCTC GCTGCCCATC 
AACCCCACCA TCATCCGCAT CATGAGGGTG CTGCGTATTG CCCGAGTGCT AAAGCTACTG 
AAGATGGCTG TGGGCATGCG GGCACTCCTG GACACAGTGA TGCAGGCCCT GCCCCAGGTG 
GGGAACTTGG GACTTCTCTT CATGTTGTTG TTTTTCATCT TTGCAGCTCT GGGCGTGGAG 
CTCTTTGGAG ATCTGGAGTG TGATGAGACG CACCCCTGCG AGGGCCTGGG CCGGCATGCC 
ACCTTTCGGA ACTTTGGCAT GGCCTTCTTG ACCCTCTTCC GAGTCTCCAC AGGTGACAAC 
TGGAATGGCA TCATGAAGGA CACCCTCCGG GACTGTGACC AGGAGTCCAC CTGCTACAAC 
ACGGTCATCT CCCCCATCTA CTTCGTTTCC TTCGTGCTGA CAGCCCAGTT TGTGCTGGTC 
AACGTGGTCA TTGCCGTGCT GATGAAGCAT CTGGAGGAGA GCAACAAGGA GGCCAAGGAG 
GAGGCCGAGC TGGAGGCTGA ACTGGAGCTA GAGATGAAGA CGCTCAGCCC ACAAGCCCCC 
TCACCACTGG GCAGCCCCTT CCTCTGGCCT GGGGTCGAGG GCCCCGACAG CCCTGGCAGC 
CCCAAACCTG GGGCCCCACA TGCCACACCC CACACTGGAG CAGCCTCCCG CTTCTCCCTG 
GAGCACCCCA TGATGGAGTC CCACCCTGAG GAGGTGCCCG TCATGCTAGG ACCAGATCTG 
CTGACTGTGC GCAAGTCTGG GGTCAGCCGA ACGCACTCTC TGCCCAATGA CAGCTACATG 
TGCCGGGATG GGAGCACTGC CGAGGGGTCC CTAGGACACA GGGGCTGGGG GCTCCCCAGA 
GCTCAGTCAG GCTCCATCTT GTCCGTCCAC TCCCAGCCAG CAGACACCAG CTACATCCCG 
CAGCTTCCCA AAGATGCGCC CCATCTGCTC CAGCCCCATG GTGCTCCCAC CTGGGGCACC 
ATCCCCAAAT TGCCCCCACC AGGCCGCTCC CCTTTGGCTC AGAGGCCACT CAGGCGCCAG 
GCAGCAATAC GGACGGATTC CCTGGACATT CAGGGCCTGG GCAGCAGGGA AGACCTGCTG 
TCAGAGGTGA GTGGGCCCTC CCCGCCGCTG GCCCGGGCCT CCTCCTTTTG GGGCCACTCC 
AGCACCCAGG CTCAGCAGCA TTCCCGCAGC CAGAGCAAGA TCTTGAAGCA CATGCCCCCG 
TCAGCCCCCT GCCCAGGCCC AGAACTCAAG TGGGCCAAGG GCCCACCAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CACGGACAGT 
CAGGAGGAAG CCCCGTCTGC ACGGGACCTG AAGAAGTGCT ACAGCGTGGA GGCGCAGAGC 
TGCCCGCGCC GGCCGGCATC CTGGCTGGAT GAACAGAGGA GGCACTCCAT CGCGGTCAGC 
TGCCTGGACA GTGGCTCCCA ACCCGCCCTG GGCCCTGATC CCTCGAGCCT TGGAGGTCAG 
CCTCTAGGGG GAGCTGGGAG CCGGCCCAAG AAGAAACTCA GCCCACCCAG CATCTCCATA 
GACCCCCCAG AGAGCCAGAG CCAGGGCCCT CGGCCCCCAC CCAGTCCTGG TGTCTGCCTC 
CGCCGGAGGG CTCCGTCCAG CGACTCCAAG GATCCCTTGG CCTCTGGCCC TCCTGACAGC 
ATGGCTGCCT CGCCCTCCCC AAAGAAAGAC GTGCTGAGTC TCTCTGGTTT ATCCTCTGAC 
CCAGCAGACC TGGACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1G
Entrez Gene ID	282411
Full Name	calcium voltage-gated channel subunit alpha1 G
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 G Names voltage-dependent T-type calcium channel subunit alpha-1G calcium channel, voltage-dependent, alpha 1G subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	CACNA1G	NP_938191.2
Danio rerio (zebrafish)	cacna1g	XP_005156372.1
Bos taurus (cattle)	CACNA1G	NP_001180069.1
Xenopus tropicalis (tropical clawed frog)	cacna1g	XP_004918544.1
Mus musculus (house mouse)	Cacna1g	NP_001106284.1
Rattus norvegicus (Norway rat)	Cacna1g	NP_113789.2
Pan troglodytes (chimpanzee)	CACNA1G	XP_511884.2
Gallus gallus (chicken)	CACNA1G	XP_004946372.1
Canis lupus familiaris (dog)	CACNA1G	XP_005624708.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1G cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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