TXNRD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TXNRD1
Entrez Gene ID	282388
Full Name	thioredoxin reductase 1
General protein information	Preferred Names thioredoxin reductase 1 Names thioredoxin reductase 1, cytoplasmic TR thioredoxin reductase TR1
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, May 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_174625.3	NP_777050.1	thioredoxin reductase 1, cytoplasmic
NM_174625.4	NP_777050.1	thioredoxin reductase 1, cytoplasmic
NM_174625.5	NP_777050.1	thioredoxin reductase 1, cytoplasmic

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TXNRD1	NP_001116141.1
	TXNRD1	XP_002798903.1
Mus musculus (house mouse)	Txnrd1	NP_001035988.1
Arabidopsis thaliana (thale cress)	GR1	NP_001118688.1
Xenopus tropicalis (tropical clawed frog)	txnrd1	NP_001243400.1
Canis lupus familiaris (dog)	TXNRD1	NP_001116145.1
Bos taurus (cattle)	TXNRD1	NP_777050.1
Homo sapiens (human)	TXNRD1	NP_001087240.1
Rattus norvegicus (Norway rat)	Txnrd1	NP_113802.2
Gallus gallus (chicken)	TXNRD1	NP_001025933.2
Oryza sativa (rice)	Os02g0813500	NP_001048485.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Thioredoxin reductase regulates the induction of haem oxygenase-1 expression in aortic endothelial cells.
Trigona WL, Mullarky IK, Cao Y, Sordillo LM
The Biochemical journal394(Pt 1)207-16(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data provide evidence for the involvement of the Trx/TrxR system, in the regulation of haem oxygenase-1 expression in aortic endothelial cells during pro-oxidant challenge
Title: Thioredoxin reductase regulates the induction of haem oxygenase-1 expression in aortic endothelial cells.

These results suggest that thioredoxin reductase may act as a positive regulator of NF-kappa B and may play an important role in the cellular inflammatory response.
Title: Overexpression of thioredoxin reductase 1 regulates NF-kappa B activation.

The following TXNRD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TXNRD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43264	NM_174625.5 Latest version!	Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OBa43264	NM_174625.3	Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa43264	NM_174625.4	Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TXNRD1 ( NM_174625.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777050.1 Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.

Price & Availability↑

CloneID	OBa43264
Clone ID Related Accession (Same CDS sequence)	NM_174625.3 , NM_174625.4 , NM_174625.5
Accession Version	NM_174625.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-21
Organism	Bos taurus(Cattle)
Product	thioredoxin reductase 1, cytoplasmic
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF053984.1, DT892402.1 and CO896976.1. On Mar 14, 2008 this sequence version replaced NM_174625.2. ##Evidence-Data-START## Transcript exon combination :: AF053984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00120237, SAMN02403640 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGGCT CCAAGGATCT CCCGGAGCCC TATGACTACG ACCTCATCAT CATTGGAGGG 
GGTTCTGGAG GACTGGCAGC CGCCAAGGAG GCAGCCAAAT ATGACAAGAA GGTGATGGTT 
CTGGATTTTG TCACTCCAAC CCCTCTTGGA ACTAGATGGG GTCTTGGAGG AACGTGTGTG 
AATGTGGGTT GCATACCTAA AAAATTGATG CACCAAGCAG CGTTGTTAGG TCAAGCCCTA 
CGAGACTCTC GAAACTATGG GTGGAACGTC GAGGAGACAG TTAAACATGA CTGGGAGCGA 
ATGACAGAGG CGGTCCAGAA CCACATTGGT TCTCTCAACT GGGGCTACCG CGTGGCTCTG 
CGGGAGAAAA AGGTCACCTA TGAGAACGCA TACGGAGAGT TTGTGGGGCC TCATAGGATT 
AAGGCAACAA ATAACAAAGG CAAAGAGAAG ATTTACTCAG CAGAGAGATT TCTCATTGCC 
ACTGGTGAAA GGCCGCGTTA TTTGGGTATC CCCGGTGACA AAGAATACTG TATCAGCAGT 
GATGATCTTT TCTCTCTACC TTATTGCCCG GGTAAGACCC TGGTGGTTGG AGCATCCTAT 
GTTGCTTTGG AATGTGCTGG ATTTCTTGCT GGTATTGGTT TAGACGTCAC TGTTATGGTA 
CGATCCATTC TCCTAAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACATATG 
CAAGAACATG GTATCAAGTT CATAAGACAG TTTGTACCAA TAAAAGTTGA ACAAATTGAA 
GCAGGGACAC CAGGCCGACT GAGAGTGATA GCTAAGTCCA CCGATAGTGA CCAAACCATC 
GAAGGAGAGT ATAATACGGT ATTGCTGGCA ATAGGAAGAG ATGCTTGCAC GAGAAAAATT 
GGCTTAGAAA ACGTGGGGGT AAAGATAAAT GAAAAGACTG GAAAAATACC TGTCACAGAG 
GAAGAGCAAA CCAACGTGCC TTACATCTAT GCCATCGGTG ATATATTGGA AGGGAAGCTG 
GAGCTCACCC CCGTGGCCAT CCAGGCAGGA AGGCTGCTGG CTCAGAGGCT GTATGGTGGC 
TCCACTGTTA AGTGTGACTA TGAAAACGTT CCAACGACTG TTTTCACTCC TTTGGAATAT 
GGGTCTTGTG GTCTTTCTGA GGAGAAAGCT GTGGAGAAAT TTGGGGAAGA AAATGTTGAG 
GTTTACCATA GTTACTTTTG GCCATTGGAA TGGACGATTC CATCAAGGGA TAACAACAAA 
TGTTACGCAA AAGTAGTCTG CAATATCAAA GACAATGAAC GTGTTGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA AGTGACCCAG GGCTTCGCAG CGGCGCTCAA ATGTGGGCTG 
ACCAAAGACC AGCTGGACAG CACCATCGGA ATCCACCCCG TTTGCGCAGA GGTGTTCACG 
ACTCTGTCGG TGACCAAGCG CTCCGGGGGA AACATCCTCC AGACCGGTTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777050.1
CDS	218..1717
Translation	MNGSKDLPEPYDYDLIIIGGGSGGLAAAKEAAKYDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALRDSRNYGWNVEETVKHDWERMTEAVQNHIGSLNWGYRVALREKKVTYENAYGEFVGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMQEHGIKFIRQFVPIKVEQIEAGTPGRLRVIAKSTDSDQTIEGEYNTVLLAIGRDACTRKIGLENVGVKINEKTGKIPVTEEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYENVPTTVFTPLEYGSCGLSEEKAVEKFGEENVEVYHSYFWPLEWTIPSRDNNKCYAKVVCNIKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKDQLDSTIGIHPVCAEVFTTLSVTKRSGGNILQTGC*G

Target ORF information:

RefSeq Version	NM_174625.3
Organism	Bos taurus(Cattle)
Definition	Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174625.3

ORF Insert Sequence:

ATGAATGGCT CCAAGGATCT CCCGGAGCCC TATGACTACG ACCTCATCAT CATTGGAGGG 
GGTTCTGGAG GACTGGCAGC CGCCAAGGAG GCAGCCAAAT ATGACAAGAA GGTGATGGTT 
CTGGATTTTG TCACTCCAAC CCCTCTTGGA ACTAGATGGG GTCTTGGAGG AACGTGTGTG 
AATGTGGGTT GCATACCTAA AAAATTGATG CACCAAGCAG CGTTGTTAGG TCAAGCCCTA 
CGAGACTCTC GAAACTATGG GTGGAACGTC GAGGAGACAG TTAAACATGA CTGGGAGCGA 
ATGACAGAGG CGGTCCAGAA CCACATTGGT TCTCTCAACT GGGGCTACCG CGTGGCTCTG 
CGGGAGAAAA AGGTCACCTA TGAGAACGCA TACGGAGAGT TTGTGGGGCC TCATAGGATT 
AAGGCAACAA ATAACAAAGG CAAAGAGAAG ATTTACTCAG CAGAGAGATT TCTCATTGCC 
ACTGGTGAAA GGCCGCGTTA TTTGGGTATC CCCGGTGACA AAGAATACTG TATCAGCAGT 
GATGATCTTT TCTCTCTACC TTATTGCCCG GGTAAGACCC TGGTGGTTGG AGCATCCTAT 
GTTGCTTTGG AATGTGCTGG ATTTCTTGCT GGTATTGGTT TAGACGTCAC TGTTATGGTA 
CGATCCATTC TCCTAAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACATATG 
CAAGAACATG GTATCAAGTT CATAAGACAG TTTGTACCAA TAAAAGTTGA ACAAATTGAA 
GCAGGGACAC CAGGCCGACT GAGAGTGATA GCTAAGTCCA CCGATAGTGA CCAAACCATC 
GAAGGAGAGT ATAATACGGT ATTGCTGGCA ATAGGAAGAG ATGCTTGCAC GAGAAAAATT 
GGCTTAGAAA ACGTGGGGGT AAAGATAAAT GAAAAGACTG GAAAAATACC TGTCACAGAG 
GAAGAGCAAA CCAACGTGCC TTACATCTAT GCCATCGGTG ATATATTGGA AGGGAAGCTG 
GAGCTCACCC CCGTGGCCAT CCAGGCAGGA AGGCTGCTGG CTCAGAGGCT GTATGGTGGC 
TCCACTGTTA AGTGTGACTA TGAAAACGTT CCAACGACTG TTTTCACTCC TTTGGAATAT 
GGGTCTTGTG GTCTTTCTGA GGAGAAAGCT GTGGAGAAAT TTGGGGAAGA AAATGTTGAG 
GTTTACCATA GTTACTTTTG GCCATTGGAA TGGACGATTC CATCAAGGGA TAACAACAAA 
TGTTACGCAA AAGTAGTCTG CAATATCAAA GACAATGAAC GTGTTGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA AGTGACCCAG GGCTTCGCAG CGGCGCTCAA ATGTGGGCTG 
ACCAAAGACC AGCTGGACAG CACCATCGGA ATCCACCCCG TTTGCGCAGA GGTGTTCACG 
ACTCTGTCGG TGACCAAGCG CTCCGGGGGA AACATCCTCC AGACCGGTTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TXNRD1 ( NM_174625.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777050.1 Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.

Price & Availability↑

CloneID	OBa43264
Clone ID Related Accession (Same CDS sequence)	NM_174625.3 , NM_174625.4 , NM_174625.5
Accession Version	NM_174625.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Bos taurus(cattle)
Product	thioredoxin reductase 1, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF053984.1, DV908554.1, EE892577.1 and CO896976.1. On May 26, 2017 this sequence version replaced NM_174625.3. ##Evidence-Data-START## Transcript exon combination :: AF053984.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGGCT CCAAGGATCT CCCGGAGCCC TATGACTACG ACCTCATCAT CATTGGAGGG 
GGTTCTGGAG GACTGGCAGC CGCCAAGGAG GCAGCCAAAT ATGACAAGAA GGTGATGGTT 
CTGGATTTTG TCACTCCAAC CCCTCTTGGA ACTAGATGGG GTCTTGGAGG AACGTGTGTG 
AATGTGGGTT GCATACCTAA AAAATTGATG CACCAAGCAG CGTTGTTAGG TCAAGCCCTA 
CGAGACTCTC GAAACTATGG GTGGAACGTC GAGGAGACAG TTAAACATGA CTGGGAGCGA 
ATGACAGAGG CGGTCCAGAA CCACATTGGT TCTCTCAACT GGGGCTACCG CGTGGCTCTG 
CGGGAGAAAA AGGTCACCTA TGAGAACGCA TACGGAGAGT TTGTGGGGCC TCATAGGATT 
AAGGCAACAA ATAACAAAGG CAAAGAGAAG ATTTACTCAG CAGAGAGATT TCTCATTGCC 
ACTGGTGAAA GGCCGCGTTA TTTGGGTATC CCCGGTGACA AAGAATACTG TATCAGCAGT 
GATGATCTTT TCTCTCTACC TTATTGCCCG GGTAAGACCC TGGTGGTTGG AGCATCCTAT 
GTTGCTTTGG AATGTGCTGG ATTTCTTGCT GGTATTGGTT TAGACGTCAC TGTTATGGTA 
CGATCCATTC TCCTAAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACATATG 
CAAGAACATG GTATCAAGTT CATAAGACAG TTTGTACCAA TAAAAGTTGA ACAAATTGAA 
GCAGGGACAC CAGGCCGACT GAGAGTGATA GCTAAGTCCA CCGATAGTGA CCAAACCATC 
GAAGGAGAGT ATAATACGGT ATTGCTGGCA ATAGGAAGAG ATGCTTGCAC GAGAAAAATT 
GGCTTAGAAA ACGTGGGGGT AAAGATAAAT GAAAAGACTG GAAAAATACC TGTCACAGAG 
GAAGAGCAAA CCAACGTGCC TTACATCTAT GCCATCGGTG ATATATTGGA AGGGAAGCTG 
GAGCTCACCC CCGTGGCCAT CCAGGCAGGA AGGCTGCTGG CTCAGAGGCT GTATGGTGGC 
TCCACTGTTA AGTGTGACTA TGAAAACGTT CCAACGACTG TTTTCACTCC TTTGGAATAT 
GGGTCTTGTG GTCTTTCTGA GGAGAAAGCT GTGGAGAAAT TTGGGGAAGA AAATGTTGAG 
GTTTACCATA GTTACTTTTG GCCATTGGAA TGGACGATTC CATCAAGGGA TAACAACAAA 
TGTTACGCAA AAGTAGTCTG CAATATCAAA GACAATGAAC GTGTTGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA AGTGACCCAG GGCTTCGCAG CGGCGCTCAA ATGTGGGCTG 
ACCAAAGACC AGCTGGACAG CACCATCGGA ATCCACCCCG TTTGCGCAGA GGTGTTCACG 
ACTCTGTCGG TGACCAAGCG CTCCGGGGGA AACATCCTCC AGACCGGTTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777050.1
CDS	218..1717
Translation	MNGSKDLPEPYDYDLIIIGGGSGGLAAAKEAAKYDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALRDSRNYGWNVEETVKHDWERMTEAVQNHIGSLNWGYRVALREKKVTYENAYGEFVGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMQEHGIKFIRQFVPIKVEQIEAGTPGRLRVIAKSTDSDQTIEGEYNTVLLAIGRDACTRKIGLENVGVKINEKTGKIPVTEEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYENVPTTVFTPLEYGSCGLSEEKAVEKFGEENVEVYHSYFWPLEWTIPSRDNNKCYAKVVCNIKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKDQLDSTIGIHPVCAEVFTTLSVTKRSGGNILQTGC*G

Target ORF information:

RefSeq Version	NM_174625.4
Organism	Bos taurus(cattle)
Definition	Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174625.4

ORF Insert Sequence:

ATGAATGGCT CCAAGGATCT CCCGGAGCCC TATGACTACG ACCTCATCAT CATTGGAGGG 
GGTTCTGGAG GACTGGCAGC CGCCAAGGAG GCAGCCAAAT ATGACAAGAA GGTGATGGTT 
CTGGATTTTG TCACTCCAAC CCCTCTTGGA ACTAGATGGG GTCTTGGAGG AACGTGTGTG 
AATGTGGGTT GCATACCTAA AAAATTGATG CACCAAGCAG CGTTGTTAGG TCAAGCCCTA 
CGAGACTCTC GAAACTATGG GTGGAACGTC GAGGAGACAG TTAAACATGA CTGGGAGCGA 
ATGACAGAGG CGGTCCAGAA CCACATTGGT TCTCTCAACT GGGGCTACCG CGTGGCTCTG 
CGGGAGAAAA AGGTCACCTA TGAGAACGCA TACGGAGAGT TTGTGGGGCC TCATAGGATT 
AAGGCAACAA ATAACAAAGG CAAAGAGAAG ATTTACTCAG CAGAGAGATT TCTCATTGCC 
ACTGGTGAAA GGCCGCGTTA TTTGGGTATC CCCGGTGACA AAGAATACTG TATCAGCAGT 
GATGATCTTT TCTCTCTACC TTATTGCCCG GGTAAGACCC TGGTGGTTGG AGCATCCTAT 
GTTGCTTTGG AATGTGCTGG ATTTCTTGCT GGTATTGGTT TAGACGTCAC TGTTATGGTA 
CGATCCATTC TCCTAAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACATATG 
CAAGAACATG GTATCAAGTT CATAAGACAG TTTGTACCAA TAAAAGTTGA ACAAATTGAA 
GCAGGGACAC CAGGCCGACT GAGAGTGATA GCTAAGTCCA CCGATAGTGA CCAAACCATC 
GAAGGAGAGT ATAATACGGT ATTGCTGGCA ATAGGAAGAG ATGCTTGCAC GAGAAAAATT 
GGCTTAGAAA ACGTGGGGGT AAAGATAAAT GAAAAGACTG GAAAAATACC TGTCACAGAG 
GAAGAGCAAA CCAACGTGCC TTACATCTAT GCCATCGGTG ATATATTGGA AGGGAAGCTG 
GAGCTCACCC CCGTGGCCAT CCAGGCAGGA AGGCTGCTGG CTCAGAGGCT GTATGGTGGC 
TCCACTGTTA AGTGTGACTA TGAAAACGTT CCAACGACTG TTTTCACTCC TTTGGAATAT 
GGGTCTTGTG GTCTTTCTGA GGAGAAAGCT GTGGAGAAAT TTGGGGAAGA AAATGTTGAG 
GTTTACCATA GTTACTTTTG GCCATTGGAA TGGACGATTC CATCAAGGGA TAACAACAAA 
TGTTACGCAA AAGTAGTCTG CAATATCAAA GACAATGAAC GTGTTGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA AGTGACCCAG GGCTTCGCAG CGGCGCTCAA ATGTGGGCTG 
ACCAAAGACC AGCTGGACAG CACCATCGGA ATCCACCCCG TTTGCGCAGA GGTGTTCACG 
ACTCTGTCGG TGACCAAGCG CTCCGGGGGA AACATCCTCC AGACCGGTTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TXNRD1 ( NM_174625.5 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777050.1 Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.

Price & Availability↑

CloneID	OBa43264
Clone ID Related Accession (Same CDS sequence)	NM_174625.3 , NM_174625.4 , NM_174625.5
Accession Version	NM_174625.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Bos taurus(cattle)
Product	thioredoxin reductase 1, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF053984.1 and CO896976.1. On Jun 19, 2018 this sequence version replaced NM_174625.4. ##Evidence-Data-START## Transcript exon combination :: AF053984.1, SRR5571263.921.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGGCT CCAAGGATCT CCCGGAGCCC TATGACTACG ACCTCATCAT CATTGGAGGG 
GGTTCTGGAG GACTGGCAGC CGCCAAGGAG GCAGCCAAAT ATGACAAGAA GGTGATGGTT 
CTGGATTTTG TCACTCCAAC CCCTCTTGGA ACTAGATGGG GTCTTGGAGG AACGTGTGTG 
AATGTGGGTT GCATACCTAA AAAATTGATG CACCAAGCAG CGTTGTTAGG TCAAGCCCTA 
CGAGACTCTC GAAACTATGG GTGGAACGTC GAGGAGACAG TTAAACATGA CTGGGAGCGA 
ATGACAGAGG CGGTCCAGAA CCACATTGGT TCTCTCAACT GGGGCTACCG CGTGGCTCTG 
CGGGAGAAAA AGGTCACCTA TGAGAACGCA TACGGAGAGT TTGTGGGGCC TCATAGGATT 
AAGGCAACAA ATAACAAAGG CAAAGAGAAG ATTTACTCAG CAGAGAGATT TCTCATTGCC 
ACTGGTGAAA GGCCGCGTTA TTTGGGTATC CCCGGTGACA AAGAATACTG TATCAGCAGT 
GATGATCTTT TCTCTCTACC TTATTGCCCG GGTAAGACCC TGGTGGTTGG AGCATCCTAT 
GTTGCTTTGG AATGTGCTGG ATTTCTTGCT GGTATTGGTT TAGACGTCAC TGTTATGGTA 
CGATCCATTC TCCTAAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACATATG 
CAAGAACATG GTATCAAGTT CATAAGACAG TTTGTACCAA TAAAAGTTGA ACAAATTGAA 
GCAGGGACAC CAGGCCGACT GAGAGTGATA GCTAAGTCCA CCGATAGTGA CCAAACCATC 
GAAGGAGAGT ATAATACGGT ATTGCTGGCA ATAGGAAGAG ATGCTTGCAC GAGAAAAATT 
GGCTTAGAAA ACGTGGGGGT AAAGATAAAT GAAAAGACTG GAAAAATACC TGTCACAGAG 
GAAGAGCAAA CCAACGTGCC TTACATCTAT GCCATCGGTG ATATATTGGA AGGGAAGCTG 
GAGCTCACCC CCGTGGCCAT CCAGGCAGGA AGGCTGCTGG CTCAGAGGCT GTATGGTGGC 
TCCACTGTTA AGTGTGACTA TGAAAACGTT CCAACGACTG TTTTCACTCC TTTGGAATAT 
GGGTCTTGTG GTCTTTCTGA GGAGAAAGCT GTGGAGAAAT TTGGGGAAGA AAATGTTGAG 
GTTTACCATA GTTACTTTTG GCCATTGGAA TGGACGATTC CATCAAGGGA TAACAACAAA 
TGTTACGCAA AAGTAGTCTG CAATATCAAA GACAATGAAC GTGTTGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA AGTGACCCAG GGCTTCGCAG CGGCGCTCAA ATGTGGGCTG 
ACCAAAGACC AGCTGGACAG CACCATCGGA ATCCACCCCG TTTGCGCAGA GGTGTTCACG 
ACTCTGTCGG TGACCAAGCG CTCCGGGGGA AACATCCTCC AGACCGGTTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777050.1
CDS	218..1717
Translation	MNGSKDLPEPYDYDLIIIGGGSGGLAAAKEAAKYDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALRDSRNYGWNVEETVKHDWERMTEAVQNHIGSLNWGYRVALREKKVTYENAYGEFVGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMQEHGIKFIRQFVPIKVEQIEAGTPGRLRVIAKSTDSDQTIEGEYNTVLLAIGRDACTRKIGLENVGVKINEKTGKIPVTEEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYENVPTTVFTPLEYGSCGLSEEKAVEKFGEENVEVYHSYFWPLEWTIPSRDNNKCYAKVVCNIKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKDQLDSTIGIHPVCAEVFTTLSVTKRSGGNILQTGC*G

Target ORF information:

RefSeq Version	NM_174625.5
Organism	Bos taurus(cattle)
Definition	Bos taurus thioredoxin reductase 1 (TXNRD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174625.5

ORF Insert Sequence:

ATGAATGGCT CCAAGGATCT CCCGGAGCCC TATGACTACG ACCTCATCAT CATTGGAGGG 
GGTTCTGGAG GACTGGCAGC CGCCAAGGAG GCAGCCAAAT ATGACAAGAA GGTGATGGTT 
CTGGATTTTG TCACTCCAAC CCCTCTTGGA ACTAGATGGG GTCTTGGAGG AACGTGTGTG 
AATGTGGGTT GCATACCTAA AAAATTGATG CACCAAGCAG CGTTGTTAGG TCAAGCCCTA 
CGAGACTCTC GAAACTATGG GTGGAACGTC GAGGAGACAG TTAAACATGA CTGGGAGCGA 
ATGACAGAGG CGGTCCAGAA CCACATTGGT TCTCTCAACT GGGGCTACCG CGTGGCTCTG 
CGGGAGAAAA AGGTCACCTA TGAGAACGCA TACGGAGAGT TTGTGGGGCC TCATAGGATT 
AAGGCAACAA ATAACAAAGG CAAAGAGAAG ATTTACTCAG CAGAGAGATT TCTCATTGCC 
ACTGGTGAAA GGCCGCGTTA TTTGGGTATC CCCGGTGACA AAGAATACTG TATCAGCAGT 
GATGATCTTT TCTCTCTACC TTATTGCCCG GGTAAGACCC TGGTGGTTGG AGCATCCTAT 
GTTGCTTTGG AATGTGCTGG ATTTCTTGCT GGTATTGGTT TAGACGTCAC TGTTATGGTA 
CGATCCATTC TCCTAAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACATATG 
CAAGAACATG GTATCAAGTT CATAAGACAG TTTGTACCAA TAAAAGTTGA ACAAATTGAA 
GCAGGGACAC CAGGCCGACT GAGAGTGATA GCTAAGTCCA CCGATAGTGA CCAAACCATC 
GAAGGAGAGT ATAATACGGT ATTGCTGGCA ATAGGAAGAG ATGCTTGCAC GAGAAAAATT 
GGCTTAGAAA ACGTGGGGGT AAAGATAAAT GAAAAGACTG GAAAAATACC TGTCACAGAG 
GAAGAGCAAA CCAACGTGCC TTACATCTAT GCCATCGGTG ATATATTGGA AGGGAAGCTG 
GAGCTCACCC CCGTGGCCAT CCAGGCAGGA AGGCTGCTGG CTCAGAGGCT GTATGGTGGC 
TCCACTGTTA AGTGTGACTA TGAAAACGTT CCAACGACTG TTTTCACTCC TTTGGAATAT 
GGGTCTTGTG GTCTTTCTGA GGAGAAAGCT GTGGAGAAAT TTGGGGAAGA AAATGTTGAG 
GTTTACCATA GTTACTTTTG GCCATTGGAA TGGACGATTC CATCAAGGGA TAACAACAAA 
TGTTACGCAA AAGTAGTCTG CAATATCAAA GACAATGAAC GTGTTGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA AGTGACCCAG GGCTTCGCAG CGGCGCTCAA ATGTGGGCTG 
ACCAAAGACC AGCTGGACAG CACCATCGGA ATCCACCCCG TTTGCGCAGA GGTGTTCACG 
ACTCTGTCGG TGACCAAGCG CTCCGGGGGA AACATCCTCC AGACCGGTTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Thioredoxin reductase regulates the induction of haem oxygenase-1 expression in aortic endothelial cells.
The Biochemical journal394(Pt 1)207-16(2006 Feb)
Trigona WL,Mullarky IK,Cao Y,Sordillo LM

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TXNRD1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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